Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.
The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.
Department of Medicine, The Johns Hopkins University, Baltimore, MD 21224, USA.
Rebekah L
RL
Gundry
Irina
I
Tchernyshyov
Shijun
S
Sheng
Yelena
Y
Tarasova
Kimberly
K
Raginski
Kenneth R
KR
Boheler
Jennifer E
JE
Van Eyk
D000818:Animals; D002460:Cell Line; D030562:Databases, Protein; D053595:Embryonic Stem Cells / Q000378:metabolism*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D020543:Proteome