A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes.
OBJECTIVE
The aim of the present study was to investigate the effect of mouse oocyte volume on the efficiency of chromosomal analysis in livestock spermatozoa.


METHODS
Oocytes were injected with bull, ram, boar and dog sperm heads, and then fused with enucleated mouse oocytes.


RESULTS
The increment of oocyte volume increased the rates of morphologically normal oocytes after sperm injection, which induced much higher rates of overall chromosome detection in bull, ram and dog spermatozoa. The recipient oocyte volume did not affect the chromosomal integrity. Furthermore, in bull, the chromosomal integrity detected by fused mouse oocytes was similar to that derived from a homologous system. On the other hand, chromosomal plates of boar spermatozoa could not be detected despite the use of fused oocytes.


CONCLUSIONS
These data indicate that fused mouse oocytes improved the efficiency of chromosome detection in bull, ram and dog spermatozoa.
Watanabe
Hiroyuki
H
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Japan. h-wata@asahikawa-med.ac.jp.
Suzuki
Hiroshi
H
Tateno
Hiroyuki
H
Fukui
Yutaka
Y
D000818:Animals; D002417:Cattle; D002869:Chromosome Aberrations; D015925:Cryopreservation; D004285:Dogs; D005260:Female; D016015:Logistic Models; D008297:Male; D051379:Mice; D008845:Microinjections; D009865:Oocytes; D012756:Sheep; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000502:physiology*