Xanthan production on polyurethane foam and its enhancement by air pressure pulsation.
In this study, we evaluated the feasibility of solid-state fermentation (SSF) on polyurethane foam (PUF) for xanthan production. The effects of air pressure pulsation (APP) on biomass accumulation and final xanthan concentration were also studied. Under suitable conditions (15% inoculum, 0.5-cm (side length) PUF cubes, 15 mL medium per gram cubes and 4.5 cm bed depth), the broth was dispersed on the PUF as a film. When the initial glucose concentration in the media was low (20 and 40 g L⁻¹), there was no significant difference between the final xanthan concentration in static SSF and submerged fermentation (SMF). When high initial glucose concentrations (60 and 80 g L⁻¹) were used, the final gum concentrations in SSF were much higher than those in SMF. When the APP technique was applied in xanthan production with a medium containing a high glucose concentration (80 g L⁻¹), the oxygen consumption rate of Xanthomonas campestris was significantly enhanced at the later stages of fermentation, and both the biomass and xanthan concentration were improved. The results indicated that SSF on PUF is suitable for xanthan preparation, especially when the initial glucose concentration ranged from 60 to 80 g L⁻¹. Those results also demonstrated that APP technology can be used to enhance xanthan yields.
Zhang
Zhi-guo
ZG
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, People's Republic China.
Chen
Hong-zhang
HZ
D000388:Air; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D005240:Feasibility Studies; D005285:Fermentation; D007101:Immersion; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011140:Polyurethanes; D011312:Pressure; D016959:Xanthomonas campestris / Q000378:metabolism
D003470:Culture Media; D011135:Polysaccharides, Bacterial; D011140:Polyurethanes; C028279:polyurethane foam; C002563:xanthan gum