Immunological one-step determination of the central nervous system indicator proteins, neuron-specific enolase and glial fibrillary acidic protein, in meat products.
The determination of specific marker proteins is important in the prevention of infections and transmission of disease. Several diagnostic assays have been developed but these are mostly restricted to the detection of single antigens. Thus there is a need for multiplex detection assays for the simultaneous analysis of several disease indicators. Consumer protection against the transmission of prion diseases is ensured by the removal of specified risk material from cattle meat during slaughtering and this is regulated by law. The investigation reported here describes a one-step determination on immunoblots of the simultaneous detection of two indicator proteins, neuron-specific enolase and glial fibrillary acidic protein, in tissues of the central nervous system. Although the two proteins run in polyacrylamide gels with similar molecular masses the indicators are differentiated by immunological reactions followed by visualizing the different coloured specific protein bands which develop. The enolase exhibits a brown band, whereas the glial fibrillary acidic protein is red. Immunoblotting has proved to be a suitable assay for multiplex analysis of marker proteins possessing similar molecular weights and is therefore a suitable tool for application in food, veterinary and medical facilities.
Institute of Hygiene, Westfälische Wilhelms-University and University Hospital Münster, Robert Koch Strasse, Münster, Germany. tkuczius@uni-muenster.de
Thorsten
T
Kuczius
Olga
O
Böhler
Helge
H
Karch
D000818:Animals; D002417:Cattle; D002490:Central Nervous System / Q000276:immunology / Q000378:metabolism*; D003257:Consumer Product Safety; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents / Q000032:analysis* / Q000378:metabolism; D008461:Meat Products / Q000032:analysis*; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000276:immunology
D005904:Glial Fibrillary Acidic Protein; D007202:Indicators and Reagents; D010751:Phosphopyruvate Hydratase