Rapid measurement of plasma free fatty acid concentration and isotopic enrichment using LC/MS.
Measurements of plasma free fatty acids (FFA) concentration and isotopic enrichment are commonly used to evaluate FFA metabolism. Until now, gas chromatography-combustion-isotope ratio mass spectrometry (GC/C/IRMS) was the best method to measure isotopic enrichment in the methyl derivatives of (13)C-labeled fatty acids. Although IRMS is excellent for analyzing enrichment, it requires time-consuming derivatization steps and is not optimal for measuring FFA concentrations. We developed a new, rapid, and reliable method for simultaneous quantification of (13)C-labeled fatty acids in plasma using high-performance liquid chromatography-mass spectrometry (HPLC/MS). This method involves a very quick Dole extraction procedure and direct injection of the samples on the HPLC system. After chromatographic separation, the samples are directed to the mass spectrometer for electrospray ionization (ESI) and analysis in the negative mode using single ion monitoring. By employing equipment with two columns connected parallel to a mass spectrometer, we can double the throughput to the mass spectrometer, reducing the analysis time per sample to 5 min. Palmitate flux measured using this approach agreed well with the GC/C/IRMS method. This HPLC/MS method provides accurate and precise measures of FFA concentration and enrichment.
Endocrine Research Unit, Mayo Clinic, Rochester, MN, USA.
Xuan-Mai T
XM
Persson
Agnieszka Urszula
AU
Blachnio-Zabielska
Michael D
MD
Jensen
D002247:Carbon Isotopes / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards
D002247:Carbon Isotopes; D005230:Fatty Acids, Nonesterified