Determining the source of equine bloodstains by dinucleotide repeats.
A novel multiplex of independent dinucleotide tandem repeat (DTR) loci was previously described that is capable of not only discriminating human and equine DNA, but of identifying a single equine source. We report a case in which a bloodstained syringe and two needles were found during inspection of a barn by inspectors of the Pennsylvania Racing Commissions. Using the multiplex and single-locus detection, all 21 equine DTR markers were detected in a suspect horse and two evidence samples, indicating the evidence samples came from the suspect animal. Only six markers were detected in the third evidence sample because the volume of blood was limited. Following whole-genome amplification and single-locus PCR, the third evidence sample detected a total of 17 markers and the likelihood of identity (probability from suspect horse/probability from a random pacer) was 7.0 × 10⁶. The DTR multiplex has some technical limitations, but it is already practical for casework.
University of Pennsylvania School of Veterinary Medicine, New Bolton Center Campus, 382 West Street Road, Kennett Square, PA 19348, USA.
Jin-Wen
JW
Chen
Cornelius E
CE
Uboh
Lawrence R
LR
Soma
Xiaoqing
X
Li
Fuyu
F
Guan
Youwen
Y
You
Ying
Y
Liu
D000827:Animal Welfare; D000818:Animals; D001801:Blood Stains; D004247:DNA / Q000302:isolation & purification; D018900:Dinucleotide Repeats; D019075:Electrophoresis, Capillary; D005787:Gene Frequency; D016678:Genome; D005838:Genotype; D006736:Horses / Q000235:genetics*; D006801:Humans; D016013:Likelihood Functions; D009339:Needles; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004247:DNA