Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences.
The centromere is essential for faithful chromosome segregation by providing the site for kinetochore assembly. Although the role of the centromere is conserved throughout evolution, the DNA sequences associated with centromere regions are highly divergent among species and it remains to be determined how centromere DNA directs kinetochore formation. Despite the active use of chicken DT40 cells in studies of chromosome segregation, the sequence of the chicken centromere was unclear. Here, we performed a comprehensive analysis of chicken centromere DNA which revealed unique features of chicken centromeres compared with previously studied vertebrates. Centromere DNA sequences from the chicken macrochromosomes, with the exception of chromosome 5, contain chromosome-specific homogenous tandem repetitive arrays that span several hundred kilobases. In contrast, the centromeres of chromosomes 5, 27, and Z do not contain tandem repetitive sequences and span non-tandem-repetitive sequences of only approximately 30 kb. To test the function of these centromere sequences, we conditionally removed the centromere from the Z chromosome using genetic engineering and have shown that that the non-tandem-repeat sequence of chromosome Z is a functional centromere.
Shang
Wei-Hao
WH
Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan.
Hori
Tetsuya
T
Toyoda
Atsushi
A
Kato
Jun
J
Popendorf
Kris
K
Sakakibara
Yasubumi
Y
Fujiyama
Asao
A
Fukagawa
Tatsuo
T
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012091:Repetitive Sequences, Nucleic Acid; D020080:Tandem Repeat Sequences
D004247:DNA