Proteome-wide analysis of protein carboxy termini: C terminomics.
As proteome-wide C-terminal sequence analysis has been largely intractable, we developed a polymer-based enrichment approach to profile protein C-terminal peptides by mass spectrometry and identified hundreds of C-terminal peptides in the Escherichia coli proteome. We isotopically labeled GluC protease-digested and undigested samples and identified GluC substrates and their cleavage sites by quantification of neo-C-terminal peptides. Our method thus enables global annotation of protein C-terminal posttranslational modifications, including proteolytic truncations.
Department of Biochemistry and Molecular Biology, University of British Columbia, Centre for Blood Research, Vancouver, British Columbia, Canada.
Oliver
O
Schilling
Olivier
O
Barré
Pitter F
PF
Huesgen
Christopher M
CM
Overall
D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D011499:Protein Processing, Post-Translational; D020543:Proteome
D029968:Escherichia coli Proteins; D020543:Proteome