Eliciting protective immune responses against murine myeloma challenge in lymphopenia mice through adoptive transfer of tumor antigen-specific lymphocytes and immunization of tumor vaccine secreting mIL-21.
Previous studies have indicated that the cytokine interleukin (IL)-21 may induce both innate and adaptive immune responses against tumors. The goal of this study was to evaluate a new adoptive immunotherapy strategy that combined lymphocytes from mice immunized with a murine myeloma vaccine secreting murine IL-21 (mIL-21-Sp2/0) in lymphopenic mice induced by cyclophosphamide. The data indicate that effective antitumor immunity was induced in mice receiving syngeneic murine lymphocytes from the mice immunized with the mIL-21-Sp2/0. More importantly, the efficacy against the Sp2/0 cell challenge was enhanced after the lymphocytes were activated and proliferated ex vivo before administration into the lymphopenic mice. We conclude that the adoptive transfer of tumor antigen-specific lymphocytes into mice immunized with mIL-21-Sp2/0 induced protective immune responses against myeloma challenge.
Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Southeast University, Nanjing, China. njdoujun@yahoo.com.cn
J
J
Dou
Y
Y
Wu
J
J
Wang
F
F
Zhao
L
L
Chu
C
C
Liu
P
P
Wen
W
W
Hu
K
K
Hu
X F
XF
He
N
N
Gu
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003520:Cyclophosphamide; D007113:Immunity, Innate; D016219:Immunotherapy, Adoptive / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology; D007378:Interleukins / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008231:Lymphopenia / Q000276:immunology* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009101:Multiple Myeloma / Q000276:immunology / Q000517:prevention & control*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D000098546:Interleukin-21
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D007378:Interleukins; D007371:Interferon-gamma; D003520:Cyclophosphamide; D000098546:Interleukin-21