Hydrophobic partitioning approach to efficient protein separation with magnetic nanoparticles.
A novel approach to the hydrophobic partitioning effect on efficient separation of protein such as BSA was demonstrated by the modification of hydrophobic pockets on the surface of silica-coated magnetic nanoparticles with various alkyl groups at various pH levels. The separation efficiency is strongly reflected and can be attained by controlling the size of the hydrophobic pocket and other factors such as the alkyl chain length, the salt concentration, and the pH levels. Furthermore, the adsorption constant (k(ad)) was calculated for the hydrophobic partitioning interaction between BSA and the hydrophobic pocket size at various pH levels.
Korea Institute of Ceramic Engineering and Technology, Seoul 153-801, South Korea. jhchang@kicet.re.kr
Jeong Ho
JH
Chang
Jiho
J
Lee
Yeonhwan
Y
Jeong
Jin
J
Hyung Lee
Ik Jin
IJ
Kim
Sang Eon
SE
Park
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide