Strong purifying selection at genes escaping X chromosome inactivation.
To achieve dosage balance of X-linked genes between mammalian males and females, one female X chromosome becomes inactivated. However, approximately 15% of genes on this inactivated chromosome escape X chromosome inactivation (XCI). Here, using a chromosome-wide analysis of primate X-linked orthologs, we test a hypothesis that such genes evolve under a unique selective pressure. We find that escape genes are subject to stronger purifying selection than inactivated genes and that positive selection does not significantly affect the evolution of these genes. The strength of selection does not differ between escape genes with similar versus different expression levels in males versus females. Intriguingly, escape genes possessing Y homologs evolve under the strongest purifying selection. We also found evidence of stronger conservation in gene expression levels in escape than inactivated genes. We hypothesize that divergence in function and expression between X and Y gametologs is driving such strong purifying selection for escape genes.
Center for Medical Genomics, Pennsylvania State University, University Park, USA.
Chungoo
C
Park
Laura
L
Carrel
Kateryna D
KD
Makova
D000818:Animals; D002460:Cell Line; D041321:Chromosomes, Human, X / Q000235:genetics*; D050172:Genes, X-Linked; D006801:Humans; D008251:Macaca / Q000235:genetics; D002679:Pan troglodytes / Q000235:genetics; D012044:Regression Analysis; D012641:Selection, Genetic; D012689:Sequence Homology, Nucleic Acid; D049951:X Chromosome Inactivation / Q000235:genetics*