Structure and gene cluster of the O-antigen of Salmonella enterica O60 containing 3-formamido-3,6-dideoxy-D-galactose.
An O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Salmonella enterica O60 strain G1462, and the following unique structure of the O-unit was determined by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy: where Fuc3NFo stands for 3-formamido-3,6-dideoxygalactose. The structure established is in agreement with the O-antigen gene cluster of S. enterica O60, which contains putative genes for the synthesis of GDP-D-Man and dTDP-D-Fuc3NFo, three glycosyltransferase genes, and two O-unit-processing genes (wzx and wzy).
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Andrei V
AV
Perepelov
Bin
B
Liu
Sof'ya N
SN
Senchenkova
Alexander S
AS
Shashkov
Lu
L
Feng
Yuriy A
YA
Knirel
Lei
L
Wang
D002240:Carbohydrate Sequence; D005643:Fucose / Q000031:analogs & derivatives* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D019779:Salmonella enterica / Q000235:genetics* / Q000378:metabolism
D019081:O Antigens; D005643:Fucose