Comparative analysis of fat and muscle proteins in fenofibrate-fed type II diabetic OLETF rats: the fenofibrate-dependent expression of PEBP or C11orf59 protein.
Fenofibrate, an agonist of PPARalpha, plays an important role in activating many proteins catalyzing lipid metabolism, and it also has a considerable effect on improvement of insulin sensitivity in the diabetic condition. To investigate fenofibrate-dependent expression of peripheral tissue proteins in diabetes, we analyzed whole muscle or fat proteins of fenofibrate-fed OLETF rats, an animal model of type II diabetes, using 2-dimensional gel electrophoresis. We found that many proteins were specifically expressed in a fenofibrate-dependent manner in these diabetic rats. In particular, a functionally unknown C11orf59 protein was differentially expressed in the muscle tissues (about 5-fold increase) in fenofibrate-fed OLETF rats as compared to control rats. Additionally, the signal proteins phosphatidylethanolamine binding protein and IkB interacting protein were differentially regulated in the fenofibrate-treated adipose tissues. We suggest here that these proteins might be involved in controlling lipid or carbohydrate metabolism in diabetes via PPARalpha activation.
Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
Jong Ryeal
JR
Hahm
Jin Sook
JS
Ahn
Hae Sook
HS
Noh
Seon Mi
SM
Baek
Ji Hye
JH
Ha
Tae Sik
TS
Jung
Yong Jun
YJ
An
Duk Kyu
DK
Kim
Deok Ryong
DR
Kim
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000273:Adipose Tissue / Q000737:chemistry* / Q000187:drug effects; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004032:Diet; D015180:Electrophoresis, Gel, Two-Dimensional; D011345:Fenofibrate / Q000008:administration & dosage*; D000960:Hypolipidemic Agents / Q000008:administration & dosage*; D009124:Muscle Proteins / Q000032:analysis* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000187:drug effects; D047493:PPAR alpha / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D020334:Rats, Inbred OLETF; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000960:Hypolipidemic Agents; C539362:Lamtor1 protein, rat; D009124:Muscle Proteins; C550693:PEBP1 protein, rat; D047493:PPAR alpha; D049488:Phosphatidylethanolamine Binding Protein; D020543:Proteome; D011993:Recombinant Fusion Proteins; D011345:Fenofibrate