Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy.
For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microfluidics to observe optically trapped living cells in a minimally invasive fashion during laser micromanipulation. The obtained results demonstrate that DHM multi-focus phase contrast provides label-free quantitative monitoring of optical manipulation with a temporal resolution of a few milliseconds.
Kemper
Björn
B
Center for Biomedical Optics and Photonics, University of Münster, Münster, Germany. bkemper@uni-muenster.de
Langehanenberg
Patrik
P
Höink
Alexander
A
von Bally
Gert
G
Wottowah
Falk
F
Schinkinger
Stefan
S
Guck
Jochen
J
Käs
Josef
J
Bredebusch
Ilona
I
Schnekenburger
Jürgen
J
Schütze
Karin
K
D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D053685:Laser Therapy / Q000379:methods; D007834:Lasers; D044085:Microfluidics; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D010789:Phototherapy / Q000379:methods; D013997:Time Factors; D014466:Ultraviolet Rays