Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis.
The acoABCL and acuABC operons of Bacillus licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the acoABCL operon is controlled by a sigmaL-dependent promoter and the acuABC operon by a sigmaA-dependent promoter. Transcription at the acoA promoter is repressed by glucose but induced by acetoin as soon as the preferred carbon source glucose is exhausted. The acuA promoter shows a similar induction pattern, but its activity is independent from the presence of acetoin. It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis.
Thanh
Trung Nguyen
TN
Institut für Pharmazie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Jürgen
Britta
B
Bauch
Melanie
M
Liebeke
Manuel
M
Lalk
Michael
M
Ehrenreich
Armin
A
Evers
Stefan
S
Maurer
Karl-Heinz
KH
Antelmann
Haike
H
Ernst
Florian
F
Homuth
Georg
G
Hecker
Michael
M
Schweder
Thomas
T
D000093:Acetoin / Q000378:metabolism*; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002072:Butylene Glycols / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic
D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000093:Acetoin