Trichloroethene and cis-1,2-dichloroethene concentration-dependent toxicity model simulates anaerobic dechlorination at high concentrations. II: continuous flow and attached growth reactors.
A model that was used to describe toxicity from high concentrations of chlorinated aliphatic hydrocarbons (CAHs) on reductively dechlorinating cultures in batch reactors (Sabalowsky and Semprini (in press)) was extended here to simulate observations in continuous flow suspended and attached growth reactors. The reductively dechlorinating anaerobic Evanite subculture (EV-cDCE) was fed trichloroethene (TCE) and excess electron donor to accumulate cis-1,2-dichloroethene (cDCE) in a continuous flow stirred tank reactor (CFSTR); and an attached growth recirculating packed column (RPC). A concentration-dependent toxicity model used to simulate the results of batch reactors in part I (Sabalowsky and Semprini (in press) Biotechnol Bioeng) also simulated well the observations for the CFSTR and RPC growth modes. The toxicity model incorporates cDCE and TCE toxicity coefficients that directly increase the cell decay coefficient in proportion with cDCE and TCE concentrations. Simulated estimates of the cDCE and TCE toxicity coefficients indicate reductively dechlorinating cells are most sensitive to high concentrations of cDCE and TCE in batch-fed growth, followed by CFSTR, with attached growth being least sensitive. The greater toxicity of TCE than cDCE, and ratio of the modeled toxicity coefficients, agrees with previously proposed models relating toxicity to partitioning in the cell wall (K(M/B)), proportional to octanol-water partitioning (K(OW)) coefficients.
Sabalowsky
Andrew R
AR
Center for Biofilm Engineering, 366 EPS Building, PO Box 173980, Montana State University, Bozeman, Montana 59717-3980, USA. andy.sabalowsky@biofilm.montana.edu
Semprini
Lewis
L
D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D041862:Chloroflexi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D004000:Dichloroethylenes / Q000378:metabolism* / Q000633:toxicity*; D010084:Oxidation-Reduction; D014241:Trichloroethylene / Q000378:metabolism* / Q000633:toxicity*
D004000:Dichloroethylenes; D014241:Trichloroethylene; C035384:1,2-dichloroethylene