Comparison of methods for the extraction of DNA from stream epilithic biofilms.
This study compares how different DNA extraction methods influence the quantity and quality of DNA yields from stream epilithic biofilms. Interpretations of bacterial community structure, using ARISA, revealed increased variability among samples processed using commercially-available kits, which also yielded lower DNA concentrations than other methods tested.
Lear
Gavin
G
Department of Soil and Physical Sciences, Lincoln University, Christchurch, New Zealand. gavin.lear@lincoln.ac.nz
Dong
Yimin
Y
Lewis
Gillian
G
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018441:Biofilms; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D017753:Ecosystem; D005618:Fresh Water / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000032:analysis; D045483:Rivers / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D012335:RNA, Ribosomal