Association of TLR7 copy number variation with susceptibility to childhood-onset systemic lupus erythematosus in Mexican population.
OBJECTIVE
Variations in gene copy number (CNV) have been recognised as a hereditable source of susceptibility in human complex diseases. Recent studies have shown that Tlr7 gene dosage has a significant contribution in the autoimmune-enhancing effect in mouse models of systemic lupus erythematosus (SLE). A study was therefore performed to investigate whether CNVs in TLR7 contribute to the genetic component of childhood-onset SLE.


METHODS
A case-control association study was performed in 328 Mexican children with SLE and 403 healthy controls. Determination of CNVs of TLR7 was achieved by real-time PCR using the ΔΔCt method. Expression levels of TLR7 and interferon α (IFNα) were determined in 23 patients. In addition, a stratification analysis was performed to investigate the association of TLR7 gene copy number (CN) with lupus nephritis.


RESULTS
A significant increase was found in the relative TLR7 gene CN in females patients with SLE compared with female controls (p<0.0001). However, logistic regression analysis by gender showed a higher OR (OR 6.61, p=0.005) in male patients with >1 copy of TLR7 than in female patients with >2 copies (OR 3.07, p<0.0001). This association was not observed with lupus nephritis. TLR7 mRNA levels correlated significantly with TLR7 CN and with IFNα mRNA levels.


CONCLUSIONS
These results show that an increase in TLR7 CN is a risk factor for childhood-onset SLE and provide new evidence for a role for X-linked gene dosage in SLE susceptibility. There is also evidence to suggest that TLR7 may be involved in the pathogenesis of SLE through the induction of IFNα.
Genomic of Complex Diseases Laboratory, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
Humberto
H
García-Ortiz
Rafael
R
Velázquez-Cruz
Francisco
F
Espinosa-Rosales
Silvia
S
Jiménez-Morales
Vicente
V
Baca
Lorena
L
Orozco
D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D005260:Female; D018628:Gene Dosage / Q000235:genetics*; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D012737:Sex Factors; D051199:Toll-Like Receptor 7 / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; C495348:TLR7 protein, human; D051199:Toll-Like Receptor 7