Protective effects of human placenta extract on cartilage degradation in experimental osteoarthritis.
This study investigated the effect of human placenta extract (HPE) on cartilage degradation in vitro MG-63 cells, articular cartilage explants, and in vivo monoiodoacetate (MIA)-induced osteoarthritis (OA). Matrix metalloproteinase (MMP)-2 activity was measured in HPE-treated osteoblastic MG-63 cells. Articular cartilage explants in rabbit were cultured, and the degree of proteoglycan (PG) degradation was assessed by measuring the amount of glycosaminoglycan (GAG) released into the culture medium. Experimental osteoarthritis was induced by intra-articular injection of 3 mg MIA in rats. Beginning 14 d post-MIA injection, HPE was administered intra-articularly once a day for 14 d. The knee joints were assessed by roentgenography, histology, and gelatinase activity. HPE inhibited PG degradation in articular cartilage explants. HPE significantly reduced deformity of knee joints and suppressed the histological change in MIA-induced OA. HPE inhibited MMP-2 activity in MG-63 cells. MMP-2 and -9 activities were also reduced in the cartilages of HPE-treated knee joints. Our results indicate that HPE has therapeutic effects on OA by protecting cartilage.
Kim
Joon-ki
JK
School of Pharmacy, Sungkyunkwan University, Gyeonggi-do 440-746, Korea.
Kim
Tae-hoon
TH
Park
Sang-won
SW
Kim
Hyo-yeon
HY
Kim
Sang hoon
Sh
Lee
Sung youl
Sy
Lee
Sun-mee
SM
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D017364:Collagenases / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007461:Iodoacetates; D007719:Knee Joint / Q000187:drug effects* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010924:Placental Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011247:Pregnancy / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D007461:Iodoacetates; D010924:Placental Extracts; D011509:Proteoglycans; D017364:Collagenases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9