An improved method for cell-to-cell transmission of infectious prion.
Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP(Sc)), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP(Sc) transmission from ScN2a cell, which persistently retains PrP(Sc), to naïve N2a cell. In this cell-to-cell transmission system, PrP(Sc) transmitted to recipient N2a cell was able to be detected within 5-7days. Further characterization showed that higher cell density greatly facilitated the transmission of PrP(Sc). This improved in vitro transmission method may become a useful tool for unveiling the molecular mechanism of PrP(Sc) transmission.
Tanaka
Masahiko
M
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Hara
Hideyuki
H
Nishina
Hiroshi
H
Hanada
Kentaro
K
Hagiwara
Ken'ichi
K
Maehama
Tomohiko
T
D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D051379:Mice; D008954:Models, Biological; D018620:PrPSc Proteins / Q000378:metabolism*; D017096:Prion Diseases / Q000378:metabolism / Q000635:transmission*; D021381:Protein Transport
D018620:PrPSc Proteins