LC-MS-MS identification and determination of the flavone-C-glucoside vicenin-2 in rat plasma samples following intraperitoneal administration of Lychnophora extract.
The flavone C-glucoside, vicenin-2, in semi-purified extracts of the leaves of Lychnophora ericoides was quantified in rat plasma samples using a method based on reversed-phase high performance liquid chromatography coupled to tandem mass spectrometry. Vicenin-2 was analyzed on a LiChrospher RP18 column using an isocratic mobile phase consisting of a mixture of methanol: water (30:70, v/v) plus 2.0% glacial acetic acid at a flow rate of 0.8 mL min(-1). Genistein was used as internal standard. The mass spectrometer was operated in positive ionization mode and analytes were quantified by multiple reaction monitoring at m/z 595 >457 for vicenin-2 and m/z 271 >153 for internal standard. Prior to the analysis, each rat plasma sample was acidified with 200 microL of 50 mmol L(-1) acetic acid solution and extracted by solid-phase extraction using a C18 cartridge. The absolute recoveries were reproducible and the coefficients of variation values were lower than 5.2%. The method was linear over the 12.5-1500 ng mL(-1) concentration range and the quantification limit was 12.5 ng mL(-1). Within-day and between-day assay precision and accuracy were studied at three concentration levels (40, 400 and 800 ng mL(-1)) and were lower than 15%. The developed and validated method seems to be suitable for analysis of vicenin-2 in plasma samples obtained from rats that receive a single i.p. dose of 200 mg kg(-1) vicenin-2 extract.
Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto-SP, Brazil, 14040-903.
Valquiria A Polisel
VA
Jabor
Denis Melo
DM
Soares
Andrea
A
Diniz
Glória Emilia Petto
GE
de Souza
Norberto Peporine
NP
Lopes
D000818:Animals; D047310:Apigenin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D007274:Injections, Intraperitoneal; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D053719:Tandem Mass Spectrometry
D005960:Glucosides; D010936:Plant Extracts; C530449:apigenin-6,8-di-C-glycopyranoside; D047310:Apigenin