Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties.
In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immunophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.
Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia. feiling_yap@imu.edu.my
Fei-Ling
FL
Yap
Soon-Keng
SK
Cheong
Radhakrishnan
R
Ammu
Chooi-Fun
CF
Leong
D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D000954:Antigens, Surface; D015415:Biomarkers; D018664:Interleukin-12