Hairpin DNA coated gold nanoparticles as intracellular mRNA probes for the detection of tyrosinase gene expression in melanoma cells.
A new intracellular mRNA imaging probe has been developed that incorporates thiol-terminated hairpin oligonucleotides covalently bound to the surface of citrate-capped gold nanoparticles. The hairpin DNA-coated gold nanoparticles (hAuNPs) positively identifies tyrosinase mRNA in cultured melanoma cells.
Department of Chemistry, Vanderbilt University, Station B351822, Nashville, TN 37235, USA.
S Reese
SR
Harry
Donna J
DJ
Hicks
Katayoun I
KI
Amiri
David W
DW
Wright
D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006046:Gold / Q000737:chemistry; D006801:Humans; D055029:Inverted Repeat Sequences; D008545:Melanoma / Q000175:diagnosis* / Q000235:genetics; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D014442:Monophenol Monooxygenase / Q000235:genetics*; D053758:Nanoparticles / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D013438:Sulfhydryl Compounds / Q000737:chemistry
D009841:Oligonucleotides; D012333:RNA, Messenger; D013438:Sulfhydryl Compounds; D006046:Gold; D014442:Monophenol Monooxygenase