Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli.
Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme beta-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-beta-D-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.
Jurkevica
Agnese
A
Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA.
Halsall
H Brian
HB
Seliskar
Carl J
CJ
Heineman
William R
WR
D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development*; D007118:Immunoassay / Q000379:methods*; D050296:Microbial Viability