Multiple nucleotide substitutions in the Neisseria meningitidis serogroup C ctrA gene cause false-negative detection by real-time PCR.
Two strains of Neisseria meningitidis serogroup C with disparate sequences of ctrA were isolated. The nucleotide substitutions did not alter the corresponding protein sequences, but they impeded the detection of these meningococcal isolates by real-time PCR.
Department of Haematology and Oncology, Unit of Clinical Microbiology, S. Orsola-Malpighi Hospital, University of Bologna, Italy.
Francesca
F
Cavrini
Giovanna
G
Liguori
Arianna
A
Andreoli
Vittorio
V
Sambri
D000328:Adult; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008589:Meningococcal Infections / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D038542:Neisseria meningitidis, Serogroup C / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D017931:DNA Primers