Myceligenerans halotolerans sp. nov., an actinomycete isolated from a salt lake, and emended description of the genus Myceligenerans.
A halotolerant actinomycete strain, designated XJEEM 11063(T), was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063(T) grew at pH 6.0-8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28-37 °C) and at salinities of 0-10 % (w/v) NaCl (optimal growth at 0-5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). MK-9(H(4)) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063(T) to the genus Myceligenerans. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense XLG9A10.2(T) (98.3 %) and Myceligenerans crystallogenes DSM 17134(T) (97.0 %). However, it had relatively low values for DNA-DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, Myceligenerans halotolerans sp. nov., is proposed. The type strain is XJEEM 11063(T) ( = DSM 21949(T) = CCTCC AA 208063(T)).
Wang
Yun
Y
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Tang
Shu-Kun
SK
The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Li
Zhi
Z
College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830046, PR China.|Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Lou
Kai
K
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
Mao
Pei-Hong
PH
Institute of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830008, PR China.
Jin
Xiang
X
Institute of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830008, PR China.
Klenk
Hans-Peter
HP
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
Zhang
Li-Xin
LX
Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, PR China.
Li
Wen-Jun
WJ
Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, PR China.|The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride