Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium.
We investigated the effects of a calmodulin (CaM) inhibitor, W-7, on the expression of lignin peroxidase (LiP) and manganese peroxidase (MnP) genes in Phanerochaete chrysosporium to consider the role of cam gene, which was upregulated in parallel with the total activities of LiP and MnP in our previous transcriptomic analysis. The addition of 100 μM W-7 to the fungal cultures repressed the total activities of LiP and MnP, whereas the addition of 100 μM W-5, which is a control drug of W-7, retained approximately half of them, indicating that the effect of W-7 was attributable to CaM inhibition. Real-time reverse transcription polymerase chain reaction analysis revealed that most of lip and mnp isozyme genes predicted from whole-genome data were significantly inhibited by W-7 at the transcription level (P ≤ 0.05). These results suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level.
The University of Shiga Prefecture, Hikone, Japan.
Takaiku
T
Sakamoto
Hironori
H
Kitaura
Masahiko
M
Minami
Yoichi
Y
Honda
Takashi
T
Watanabe
Akio
A
Ueda
Kazumi
K
Suzuki
Toshikazu
T
Irie
D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D002147:Calmodulin / Q000378:metabolism*; D017931:DNA Primers; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D016681:Genome, Fungal; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D020075:Phanerochaete / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D017931:DNA Primers; D005656:Fungal Proteins; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D017871:Calcium-Calmodulin-Dependent Protein Kinases