Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens.
Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN-gamma) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4(+) T cells and IFN-gamma production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4(+) Tem and long-lived CD4(+) Tcm cells.
CIRAD, Campus International de Baillarguet, Montpellier Cedex 5, France. philippe.totte@cirad.fr
Philippe
P
Totté
Arshad
A
Mather
Lina
L
Reslan
Yvan
Y
Boublik
Mamadou
M
Niang
Dion
D
Du Plessis
Laurence
L
Dedieu
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D049109:Cell Proliferation; D018604:Epitope Mapping; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000378:metabolism; D009176:Mycoplasma mycoides / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000276:immunology* / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007371:Interferon-gamma