The ethylene signaling pathway has a negative impact on sucrose-induced anthocyanin accumulation in Arabidopsis.
In an attempt to understand the complex regulatory mechanisms underlying sucrose-induced flavonoid biosynthesis, we examined several Arabidopsis mutants with altered anthocyanin accumulation. We determined that disruption of ethylene signaling results in a dramatic increase in sucrose-induced anthocyanin accumulation. Furthermore, we investigated why the ein2-1 (ethylene insensitive) Arabidopsis mutant accumulates higher levels of anthocyanin in response to sucrose than wild-type Arabidopsis. An increased level of PAP1 transcript in the ein2-1 mutant appears to be the main factor responsible for the increased accumulation of anthocyanin in response to sucrose. Therefore, our results indicate that the ethylene signaling pathway plays a negative role in sucrose-induced anthocyanin accumulation. We believe that the explanation for this observation may be related to the initiation of the senescence program in plants.
College of Life Sciences and Biotechnology, Korea University, 1, 5-ka Anam-dong, Sungbuk-ku, Seoul 136-713, Republic of Korea.
Yerim
Y
Kwon
Jee Eun
JE
Oh
Hana
H
Noh
Suk-Whan
SW
Hong
Seong Hee
SH
Bhoo
Hojoung
H
Lee
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000872:Anthocyanins / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D002734:Chlorophyll / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D012835:Silver Nitrate / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000217:Acyltransferases; D000872:Anthocyanins; D029681:Arabidopsis Proteins; D002734:Chlorophyll; D005030:Ethylenes; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger; D012835:Silver Nitrate; D013395:Sucrose; D014157:Transcription Factors; C502612:AT1G56650 protein, Arabidopsis; C000612141:REG3A protein, human; C036216:ethylene; C491135:AT5G13930 protein, Arabidopsis