[Evidence of intraepithelial lymphocytes through immunocytochemistry stains in children with celiac disease].
The purpose of of this study is to identify IELs through immunocytochemistry stains for counting infiltration duodenal mucosa with T lymphocytes in children with celiac disease.


METHODS
It was selected up simptomatology (abdominal pain, anorexia, failure thrive, chronic diarheea), features immunology(positifs tissue transglutaminase antibodies) and considering the histopathologic results (IELs > 30/100 enterocytes, crypt hyperplasia and villous atrophy) we made two groups: first group (13 children with celiac disease of 53 children with celiac disease) and second group (13 children with chronic duodenitis nonspecific). For evidence of intraepithelial lymphocytes T using immunocytochemistry tehnicque (immunohistochemical staining) Envision with antibody anti CD 3 from Dako firme. Corresponding Marsh clasification considering patological IELs > 30/100 enterocytes. Statitics method was used The Student' t test.


RESULTS
Analyse IELs in two groups our show difference statistics to demonstrate with the Student' t test (t = -12.237; p = 0.003).


CONCLUSIONS
An increase IELs is helpful in recognising early and bordeline, but the finding is not specific. The histopathologic exam remains the gold standard for celiac disease.
Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi Facultatea de Medicină, Clinica a III(-a) Pediatrie.
Silvia
S
Ghimpu
Laura
L
Bozomitu
E
E
Cîrdei
Doina
D
Mihlăilă
D
D
Boldureanu
D
D
Moraru
D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology*; D007958:Leukocyte Count; D013601:T-Lymphocytes / Q000276:immunology*
D015415:Biomarkers; D004396:Coloring Agents