Expression changes and roles of matrix metalloproteinases in a rat model of traumatic deep vein thrombosis.
OBJECTIVE
To study the expression changes of matrix metalloproteinases (MMPs) in traumatic deep vein thrombosis (TDVT) in a rat model with the aid of gene chip technology and to explore the roles of MMPs in TDVT.


METHODS
Totally 150 Sprague Dawley rats were randomly divided into control group (n equal to 10) and model group (n equal to 140). Rat models of TDVT were established by clamping the femoral vein and fixing the bilateral hind limbs. Then fixation of the hip spica with plaster bandage was conducted. According to the observation phases and/or biological situations of the femoral vein thrombosis, the model rats were further divided into 7 groups. Vascular tissues were obtained from each group through noninvasive incision into the femoral vein at corresponding time points. We adopted the Trizol one-step method for total RNA extraction, Affymetrix RAT 230 2.0 array for detection of RNA expressions and fold change (FC) analysis for changes of differential expressions of MMPs in each group. The main outcome parameters measured included expressions of MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-16, MMP-23 and MMP-24. Gene array data of these MMPs were analyzed by the Affymetrix Microarray Analysis software (Version 5.0).


RESULTS
FC analysis showed differential expressions of MMPs in each group during the course of TDVT. At the initial period of thrombosis, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, and MMP-24 had significantly high expression, while MMP-12, MMP-13, MMP-14, MMP-16 and MMP-23 had relatively low expression. MMPs were all highly expressed at the peak time of thrombosis. In the process of thrombus resolution, MMP-2, MMP-10, MMP-16 and MMP-24 have relatively low expression, while MMP-12, MMP-13, MMP-14, MMP-16 and MMP-23 have significantly high expression.


CONCLUSIONS
MMPs may affect the process of TDVT through transcription regulation of the fibrinolysis-anti-fibrinolytic system during the course of thrombosis and thrombus resolution.
Zhang
Yu-bing
YB
Department of Orthopaedics, First Affiliated Hospital of Kunming Medical College, China.
Li
Wen
W
Yao
Li-qing
LQ
Zhao
Xue-ling
XL
Wang
Bing
B
Li
Hong-kun
HK
Ning
Ya
Y
Song
En
E
Zhang
Xin-xin
XX
D000818:Animals; D004195:Disease Models, Animal; D020782:Matrix Metalloproteinases / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020246:Venous Thrombosis / Q000201:enzymology / Q000209:etiology*; D014947:Wounds and Injuries / Q000150:complications*
D020782:Matrix Metalloproteinases