A simple fluorescent strategy for in situ evaluation of cell surface carbohydrate with a quantum dot-lectin nanoprobe.
A simple and rapid fluorescent method was developed for the in situ evaluation of cell surface carbohydrate by homogeneous specific recognition of a quantum dot-lectin nanoprobe to mannosyl groups on the cell surface. The strategy was further used for dynamically monitoring the alteration of cell surface carbohydrate expression in response to drugs.
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
Xu
X
Xu
Lin
L
Ding
Yadong
Y
Xue
Huangxian
H
Ju
D019187:Cadmium Compounds / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D053758:Nanoparticles / Q000737:chemistry*; D011422:Propionates / Q000737:chemistry; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D002241:Carbohydrates; D005456:Fluorescent Dyes; D011422:Propionates; D003208:Concanavalin A; D013691:Tellurium; C028337:cadmium telluride