Lymphoproliferative response to herpes simplex virus type 1, cytomegalovirus, Epstein-Barr virus, varicella zoster virus, human herpes virus 6, 7, and 8 antigen stimulation in pediatric allogeneic stem cell transplant recipients.
We evaluate the recovery of CMI to various herpes viruses by measuring in vitro LPR to specific recall antigens. CMI was evaluated by the in vitro LPR of PBMC to specific purified HSV-1, VZV, CMV, EBV, HHV-6, -7, -8, antigens. Results were expressed as SI. SI > or = 3 was regarded as positive LPR. Serial measurements were taken prospectively from pretransplant till 12-month post-transplant. Thirty-six patients (M = 19; F = 17) with median age 10.5 yr old were recruited. Most transplants were from MSD with PBSC as the stem cell source. Altogether 50% of subjects started to show positive LPR to HSV-1, CMV, and VZV antigens at two-month post-transplant, major upsurges were noted until 6-month post-transplant. Subjects showed positive LPR to EBV, HHV-6, HHV-7, and HHV-8 antigens were all along <50% throughout the study period. The antibody status of donor and recipient for HSV-1, CMV, and VZV were associated with the timing of recovery of CMI. Choice of donor and stem cell source were important determinants of eventual LPR to various herpes viruses at 3-month post-transplant. At 12-month post-transplant, there was no statistical difference in any parameters in affecting LPR to different herpes viruses.
Department of Pediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China. frankiecheng@cuhk.edu.hk
Frankie Wai Tsoi
FW
Cheng
Paul Kay Sheung
PK
Chan
Vincent
V
Lee
Wing Kwan
WK
Leung
Ming Kong
MK
Shing
Chi Kong
CK
Li
Ting Fan
TF
Leung
D000293:Adolescent; D000942:Antigens, Bacterial / Q000276:immunology*; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D003586:Cytomegalovirus Infections / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000276:immunology; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control; D019337:Hematologic Neoplasms / Q000601:surgery; D018380:Hematopoietic Stem Cell Transplantation; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology; D015654:Herpesvirus 6, Human / Q000276:immunology; D016199:Herpesvirus 7, Human / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007223:Infant; D008208:Lymphatic System / Q000276:immunology*; D008297:Male; D019172:Transplantation Conditioning
D000942:Antigens, Bacterial