Prevention of UV-induced skin damages by 11,14,17-eicosatrienoic acid in hairless mice in vivo.
Polyunsaturated fatty acids (PUFAs) are known to play important roles in various physiological and pathological processes. Recent studies have shown that some omega-3 (omega-3) PUFAs, such as eicosapentaenoic acid (EPA) and dodecahexaenoic acid (DHA), have protective effects on acute and chronic UV-induced changes. However, the effects of other omega-3 PUFAs including 11,14,17-eicosatrienoic acid (20:3) (ETA) on UV-induced skin damages are poorly understood. In this study, we investigated the cutaneous photoprotective effects of ETA in hairless mice in vivo. Female HR-1 hairless mice were topically treated with vehicle (ethanol:polyethylene glycol=30:70) only, 0.1% ETA, or 1% ETA once a day for 3 successive days after one time UV irradiation (200 mJ/cm(2)) on dorsal skins. Skin biopsy was carried out on the fourth day (72 hr after UV irradiation). We found that topical treatment with ETA attenuated UV-induced epidermal and dermal thickness and infiltration of inflammatory cells, and impairment of skin barrier function. In addition, ETA suppressed the expression of IL-1beta, COX-2, and MMP-13 induced by UV irradiation. Our results show that the topical application of ETA protects against UV-induced skin damage in hairless mice and suggest that ETA can be a potential agent for preventing and/or treating UV-induced inflammation and photoaging.
Department of Dermatology, Seoul Nationa University College of Medicine, Seoul, Korea.
Xing-Ji
XJ
Jin
Eun Ju
EJ
Kim
In Kyung
IK
Oh
Yeon Kyung
YK
Kim
Chi-Hyun
CH
Park
Jin Ho
JH
Chung
D015126:8,11,14-Eicosatrienoic Acid / Q000008:administration & dosage*; D000287:Administration, Topical; D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D012871:Skin Diseases / Q000473:pathology / Q000517:prevention & control*; D014466:Ultraviolet Rays
D053583:Interleukin-1beta; D011837:Radiation-Protective Agents; C034152:11,14,17-eicosatrienoic acid; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; D015126:8,11,14-Eicosatrienoic Acid