Swine virus-specific cytotoxic cells producing IFNgamma under different conditions of virus antigenic stimulation.
Flow cytometry is a useful tool to determine both the phenotype and function of immune cells during vaccination and viral infection. Since cytokine release is a hallmark of cell activation, detection of intracellular interferon (IFN)gamma in cytotoxic CD8alpha+ cells under physiological conditions and after Aujeszky's Disease Virus (ADV)- or Porcine Circovirus type 2 (PCV2)-swine interaction was carried out. Blood samples were collected from healthy 10-month-old pigs, ADV- or PCV2-vaccinated pigs, and PCV2-infected pigs; the levels of total IFNgamma+ and CD8alpha+IFNgamma+ cells were evaluated after PMA-ionomycin and virus-specific in vitro stimulation. High CD8alpha+IFNgamma+ cell levels were detected in adult pigs, whereas lower virus-specific cell fractions were observed after ADV or PCV2 vaccination as well as after PCV2 natural infection due to restricted activation. Such results support the use of cytokine intracellular staining to monitor virus-specific cell-mediated immunity.
Dipartimento di Salute Animale, Università degli Studi di Parma, Parma, Italy. luca.ferrari@unipr.it
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Ferrari
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De Angelis
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Martelli
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Borghetti
D000818:Animals; D000956:Antigens, Viral / Q000502:physiology*; D002478:Cells, Cultured; D017925:Circovirus / Q000145:classification / Q000502:physiology*; D005434:Flow Cytometry; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D015759:Ionomycin; D007963:Leukocytes, Mononuclear / Q000821:virology*; D011557:Pseudorabies / Q000517:prevention & control; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology
D000956:Antigens, Viral; D014765:Viral Vaccines; D015759:Ionomycin; D007371:Interferon-gamma