Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset.
OBJECTIVE
To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach.


METHODS
Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach.


RESULTS
Five hundred fifty-nine lipid features differentiated (q<0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus.


CONCLUSIONS
A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization.
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Christina M
CM
Sorensen
Jie
J
Ding
Qibin
Q
Zhang
Thierry
T
Alquier
Rui
R
Zhao
Patricia W
PW
Mueller
Richard D
RD
Smith
Thomas O
TO
Metz
D000293:Adolescent; D000328:Adult; D002648:Child; D002853:Chromatography, Liquid; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D055815:Young Adult
D008055:Lipids