Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation.
We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.
Shinya
S
Nakamura
Shoji
S
Mano
Yuji
Y
Tanaka
Masato
M
Ohnishi
Chihiro
C
Nakamori
Masami
M
Araki
Tomoko
T
Niwa
Mikio
M
Nishimura
Hironori
H
Kaminaka
Tsuyoshi
T
Nakagawa
Yutaka
Y
Sato
Sumie
S
Ishiguro
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004351:Drug Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D014170:Transformation, Genetic
D005819:Genetic Markers; D009943:Organophosphorus Compounds; C003122:bialaphos