APOBEC3G directly binds Hepatitis B virus core protein in cell and cell free systems.
APOBEC3G (A3G) is an intrinsic antiretroviral factor which can inhibit Hepatitis B virus (HBV) replication. This antiviral activity mainly depends on A3G incorporation into viral particles. However, the mechanisms of A3G packaging into HBV particles have not been well characterized. In this paper, we demonstrated that A3G interacted with the HBV core protein (HBc) directly in co-transfected HepG2 cells using the fluorescence resonance energy transfer (FRET) approach. In addition, we further found that this interaction did not require other factors in vitro using surface plasmon resonance (SPR) technology on BIAcore 3000. While cellular RNA or viral RNA was added to A3G protein solution before flow through the BIAcore chip, the interaction was not affected. In conclusion, these results suggest the possibility that A3G is incorporated into HBV viral particles via direct binding with HBc protein.
Zhao
Dongjiu
D
Department of Anatomy, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
Wang
Xianfeng
X
Lou
Guohua
G
Peng
Guoping
G
Li
Jie
J
Zhu
Haihong
H
Chen
Feng
F
Li
Shuping
S
Liu
Dongcheng
D
Chen
Zhi
Z
Yang
Zhenggang
Z
D000071480:APOBEC-3G Deaminase; D002460:Cell Line; D002474:Cell-Free System; D003564:Cytidine Deaminase / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D006512:Hepatitis B Core Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000276:immunology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D014779:Virus Replication
D006512:Hepatitis B Core Antigens; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase