Entrophospora nevadensis, a new arbuscular mycorrhizal fungus from Sierra Nevada National Park (southeastern Spain).
A new fungal species in the arbuscular mycorrhiza-forming Glomeromycetes, Entrophospora nevadensis, was isolated from soil near the roots of several endemic and endangered plant species (e.g. Plantago nivalis and Alchemilla fontqueri) growing in Sierra Nevada National Park (Granada, Andalucia, Spain). The fungus was propagated in trap cultures on Plantago nivalis and Sorbus hybrida and in pure cultures on Trifolium pratense and Sorghum vulgare. Spores are yellow brown to brown, 90-115 .m diam and form singly in soil, in the neck of adherent sporiferous saccules that form either terminally or intercalary on mycelial hyphae. Spores have two three-layered walls and conspicuous, 6-12 microm long, spiny, thorn-like projections on the outer wall consisting of hyaline to subhyaline, evanescent tips and yellow brown to brown, persistent bases. In aging spores these projections are usually shorter (1-2.8 microm) and dome-shaped or rounded, sometimes with a central pit on top where the evanescent tip has sloughed off. Molecular analysis with partial sequences of the 18S ribosomal gene places the fungus within the Diversisporales. The new fungus was found in soil near plants with different living strategies but growing in high altitude soils with acidic pH, high soil moisture and organic carbon content, and close to streams.
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008 Granada, Spain.
Javier
J
Palenzuela
José Miguel
JM
Barea
Nuria
N
Ferrol
Concepción
C
Azcón-Aguilar
Fritz
F
Oehl
D004271:DNA, Fungal / Q000032:analysis; D056727:Endangered Species; D055137:Glomeromycota / Q000145:classification* / Q000235:genetics / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D016533:Mycological Typing Techniques; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics / Q000502:physiology; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D010943:Plantago / Q000382:microbiology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D027824:Rosaceae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013030:Spain; D013045:Species Specificity; D013172:Spores, Fungal / Q000502:physiology; D013559:Symbiosis
D004271:DNA, Fungal; D012337:RNA, Ribosomal, 18S; D012987:Soil