Long-term effect of carbodiimide on dentin matrix and resin-dentin bonds.
OBJECTIVE
To characterize the interaction of 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on the resin-dentin bond.


METHODS
Changes to the stiffness of demineralized dentin fragments treated with EDC/N-hydroxysuccinimide (NHS) in different solutions were evaluated at different time points. The resistance against enzymatic degradation was indirectly evaluated by ultimate tensile strength (UTS) test of demineralized dentin treated or not with EDC/NHS and subjected to collagenase digestion. Short- and long-term evaluations of the strength of resin-dentin interfaces treated with EDC/NHS for 1 h were performed using microtensile bond strength (microTBS) test. All data (MPa) were individually analyzed using ANOVA and Tukey HSD tests (alpha = 0.05).


RESULTS
The different exposure times significantly increased the stiffness of dentin (p < 0.0001, control-5.15 and EDC/NHS-29.50), while no differences were observed among the different solutions of EDC/NHS (p = 0.063). Collagenase challenge did not affect the UTS values of EDC/NHS group (6.08) (p > 0.05), while complete degradation was observed for the control group (p = 0.0008, control-20.84 and EDC/NHS-43.15). EDC/NHS treatment did not significantly increase resin-dentin muTBS, but the values remained stable after 12 months water storage (p < 0.05).


CONCLUSIONS
Biomimetic use of EDC/NHS to induce exogenous collagen cross-links resulted in increased mechanical properties and stability of dentin matrix and dentin-resin interfaces.
Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois 60612, USA. bedran@uic.edu
Ana Karina B
AK
Bedran-Russo
Cristina M P
CM
Vidal
Paulo H
PH
Dos Santos
Carina S
CS
Castellan
D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D001840:Dental Bonding; D003804:Dentin / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004548:Elasticity; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D008963:Molar / Q000033:anatomy & histology / Q000737:chemistry; D015394:Molecular Structure; D019279:Resin Cements / Q000737:chemistry* / Q000378:metabolism; D013314:Stress, Mechanical; D013388:Succinimides / Q000737:chemistry; D013718:Tensile Strength
D001672:Biocompatible Materials; D019279:Resin Cements; D013388:Succinimides; C032620:succinimide; D003094:Collagen; D005022:Ethyldimethylaminopropyl Carbodiimide