Allred scoring for ER reporting and it's impact in clearly distinguishing ER negative from ER positive breast cancers.
OBJECTIVE
To determine the scoring of Estrogen Receptor (ER) status in carcinoma breast by Allred method that is essentially bimodal and to compare the results with a conventional scoring system.


METHODS
A retrospective, comparative study carried out at Aga Khan University Hospital Section of Histopathology over a period of 18 months, i.e., Jan 2005 to June 2006. Anti ER antibody (clone D07) was used for all IHC stains using envision detection system. ER stains of 860 consecutive breast cancer cases were reviewed and rescored by both conventional and Allred method of ER scoring.


RESULTS
Comparison of results showed that there was a substantial decrease in weak positive cases from 18% to 5% by rescoring using Allred scoring system compared to conventional scoring. The data was analyzed using chi square test.


CONCLUSIONS
The sensitivity and specificity of Allred method were calculated; Sensitivity of Allred method was 99.4% & Specificity of Allred method was 99.5% whereas sensitivity and specificity of conventional method was 88.0% and 84% respectively
Department of Pathology, Shaukat Khanum Cancer Hospital, Lahore.
Asim
A
Qureshi
Shahid
S
Pervez
D000911:Antibodies, Monoclonal / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D011960:Receptors, Estrogen / Q000032:analysis* / Q000145:classification / Q000276:immunology; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen