Higher toxicity of dibutyltin and poly-L-lactide with a large amount of tin but lower toxicity of poly-L-lactide of synthetic artificial dura mater exhibited on murine astrocyte cell line.
Neurotoxicities of dibutyltin (DBT), tin(II) octylate (OT), poly-L-lactides (PLLA, molecular weight [MW]=5000, PLLA 5000), PLLA without tin (MW=3000, PLLA 3000), PLLA with a large amount (590 ppm) of tin (S3), poly(glycolic acid-co-epsilon-caprolactone) oligomer (MW=6200, PGC oligomer), and poly(L-lactic acid-co-glycolic acid-co-epsilon-caprolactone) oligomer (MW=6400, PLGC oligomer) related to artificial dura mater were examined using the murine astrocyte cell line, CRL-2534. The indices were cell viability, glutamate concentration in the cell supernatant, and cell proliferation. Lower cell viability was observed among cells exposed to 0.5 microM DBT or 10 microg/ml of S3. There were no differences in cell viability of astrocytes exposed to OT, PLLA 5000, PLLA 3000, PGC oligomer, or PLGC oligomer. Mean glutamate concentration in the supernatant of cells exposed to 0.25 muM DBT was higher than that of the control after 2 h incubation. Lower mean concentration of glutamate in the supernatant of cells exposed to 5 microg/ml of S3 was observed after 2 h incubation. Cells exposed to 50 microg/ml of PGC oligomer had a higher mean concentration of glutamate in the supernatant. OT only inhibited cell proliferation at 100 microM. Proliferation of cells exposed to 0.25 microM or 0.5 microM DBT was inhibited, as was that of cells exposed to 100 microM OT, 50 microg/ml PLLA 5000, 50 microg/ml PLLA 3000, and 5 microg/ml S3, 5 d and 7 d after exposure. Although DBT does not reach levels that induced neurotoxicity in artificial dura mater, these results suggest that DBT is neurotoxic and PLLA toxicity increases with the increase in tin concentration.
Department of Hygiene and Preventive Medicine, Fukushima Medical University.
Masayoshi
M
Tsuji
Yoko
Y
Inoue
Chiemi
C
Sugaya
Masashi
M
Tsunoda
Tsukiko
T
Sugaya
Masami
M
Takahashi
Toshiyasu
T
Yuba
Toshie
T
Tsuchiya
Yoshiharu
Y
Aizawa
D020341:Absorbable Implants; D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D001705:Bioprosthesis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004388:Dura Mater; D005971:Glutamates / Q000494:pharmacology; D051379:Mice; D009947:Organotin Compounds / Q000737:chemistry / Q000633:toxicity*; D011091:Polyesters / Q000737:chemistry / Q000633:toxicity*; D017971:Tin Compounds / Q000633:toxicity*
D005971:Glutamates; D009947:Organotin Compounds; D011091:Polyesters; D017971:Tin Compounds; C056189:di-n-butyltin; C033616:poly(lactide)