The envelope glycoprotein domain III of Dengue virus type 2 induced the expression of anticoagulant molecules in endothelial cells.
Dengue virus (DV) causes a non-specific febrile illness known as Dengue fever (DF), and a severe life-threatening illness, Dengue hemorrhagic fever/Dengue shock syndrome (DHF/DSS). Hemostatic changes induced by this virus involve three main factors: thrombocytopenia, endothelial cell damage, and significant abnormalities of the coagulation and fibrinolysis systems. The pathogenesis of bleeding in DV infections remains unknown. In this article, we focused on the DV activating endothelial cells and altering the parameters of hemostasis system. The expression of hemostasis-related factors, Thrombomodulin, TF, TFPI, t-PA, and PAI-1, in DV-infected cells were determined by RT-PCR. Flow cytometry analysis and immunofluorescence staining confirmed that the expression levels of TM in the DV-infected HMEC-1 and THP-1 cells were increased. In addition, the purified recombinant domain III of the envelope glycoprotein of DV (EIII) could induce the expression of TM in the HMEC-1 cells and THP-1 cells. The TM expression induced by DV or EIII in the endothelial cells and monocytic cells suggests that the EIII of DV plays an important role in the pathogenesis of DHF/DSS.
Department of Health, Bureau of Foods and Drug Analysis, Tainan, Taiwan, ROC.
Lien-Cheng
LC
Chen
Trai-Ming
TM
Yeh
Yi-Ying
YY
Lin
Yi-Fen
YF
Wang
Shu-Jem
SJ
Su
Chang-Yu
CY
Chen
Kuan-Hua
KH
Lin
Miao-Chen
MC
Chou
Huey-Wen
HW
Shyu
D000925:Anticoagulants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D003715:Dengue / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006487:Hemostasis; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
D000925:Anticoagulants; D015415:Biomarkers; C051821:E-glycoprotein, Dengue virus type 2; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins