OIP5 is a highly expressed potential therapeutic target for colorectal and gastric cancers.
Previously, we reported that overexpression of Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) caused multi-septa formation and growth defects, both of which are considered cancer-related phenotypes. To evaluate OIP5 as a possible cancer therapeutic target, we examined its expression level in 66 colorectal cancer patients. OIP5 was upregulated about 3.7-fold in tumors and over 2-fold in 58 out of 66 colorectal cancer patients. Knockdown of OIP5 expression by small interfering RNA specific to OIP5 (siOIP5) resulted in growth inhibition of colorectal and gastric cancer cell lines. Growth inhibition of SNU638 by siOIP5 caused an increase in sub-G1 DNA content, as measured by flow cytometry, as well as an apoptotic gene expression profile. These results indicate that knockdown of OIP5 may induce apoptosis in cancer cells. Therefore, we suggest that OIP5 might be a potential cancer therapeutic target, although the mechanisms of OIP5-induced carcinogenesis should be elucidated.
Chun
Ho-Kyung
HK
Medical Genomics Research Center, KRIBB, Daejeon, Korea.
Chung
Kyung-Sook
KS
Kim
Hee Cheol
HC
Kang
Jung-Eun
JE
Kang
Min Ah
MA
Kim
Jong-Tae
JT
Choi
Eun Hwa
EH
Jung
Kyeong-Eun
KE
Kim
Moon-Hee
MH
Song
Eun Young
EY
Kim
Seon-Young
SY
Won
Misun
M
Lee
Hee Gu
HG
D000818:Animals; D017209:Apoptosis; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D020869:Gene Expression Profiling; D006801:Humans; D046228:Microarray Analysis; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D015854:Up-Regulation
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C517146:OIP5 protein, human; D034741:RNA, Small Interfering