Cloning of a novel dehalogenase from environmental DNA.
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.
Kimoto
Hisashi
H
Department of Bioscience, Fukui Prefectural University, Fukui, Japan. kimoto@fpu.ac.jp
Suye
Shin-ichiro
S
Makishima
Hirokazu
H
Arai
Jun-ichirou
J
Yamaguchi
Sachiko
S
Fujii
Yutaka
Y
Yoshioka
Toshihito
T
Taketo
Akira
A
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004777:Environment; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004247:DNA; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase