Epstein-Barr virus lytic cycle activation alters proteasome subunit expression in Burkitt's lymphoma cells.
We have shown that Epstein-Barr virus (EBV) lytic cycle activation in Burkitt's lymphoma (BL) cells down-regulates chymotrypsin- and caspase-like activities of the proteasome. The aim of the present study was to evaluate whether EBV activation might also affect proteasome subunit composition. Our results indicate that, independently of the latency program established in the host cells, induction of the EBV lytic cycle reduces the expression of the proteasomal components β5, β1 and β2i, whereas it increases that of β2, β1i, PA28α and PA28β. The modulation of the composition and enzymatic activities of the proteolytic complex are indicative of a less efficient generation of viral immunoepitopes.
De Leo
Alessandra
A
Department of Public Health Sciences, University &#x2018;La Sapienza&#x2019;, P. le A. Moro 5, I-00185 Rome, Italy.
Matusali
Giulia
G
Arena
Giuseppe
G
Di Renzo
Livia
L
Mattia
Elena
E
D002051:Burkitt Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014775:Virus Activation / Q000502:physiology*
D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex