[Evidence of intraepithelial lymphocytes through immunocytochemistry stains in children with celiac disease].
The purpose of of this study is to identify IELs through immunocytochemistry stains for counting infiltration duodenal mucosa with T lymphocytes in children with celiac disease.


METHODS
It was selected up simptomatology (abdominal pain, anorexia, failure thrive, chronic diarheea), features immunology(positifs tissue transglutaminase antibodies) and considering the histopathologic results (IELs > 30/100 enterocytes, crypt hyperplasia and villous atrophy) we made two groups: first group (13 children with celiac disease of 53 children with celiac disease) and second group (13 children with chronic duodenitis nonspecific). For evidence of intraepithelial lymphocytes T using immunocytochemistry tehnicque (immunohistochemical staining) Envision with antibody anti CD 3 from Dako firme. Corresponding Marsh clasification considering patological IELs > 30/100 enterocytes. Statitics method was used The Student' t test.


RESULTS
Analyse IELs in two groups our show difference statistics to demonstrate with the Student' t test (t = -12.237; p = 0.003).


CONCLUSIONS
An increase IELs is helpful in recognising early and bordeline, but the finding is not specific. The histopathologic exam remains the gold standard for celiac disease.
Ghimpu
Silvia
S
Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi Facultatea de Medicină, Clinica a III(-a) Pediatrie.
Bozomitu
Laura
L
Cîrdei
E
E
Mihlăilă
Doina
D
Boldureanu
D
D
Moraru
D
D
D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology*; D007958:Leukocyte Count; D013601:T-Lymphocytes / Q000276:immunology*
D015415:Biomarkers; D004396:Coloring Agents