Lack of association between the GRP78 polymorphisms in the promoter and 3' UTR and susceptibility to chronic HBV infection in a Chinese Han population.
BACKGROUND
Hepatitis B virus (HBV) infection causes large amount of unfolding or false-folding protein accumulation in the endoplasmic reticulum (ER), which in turn induces the expression of glucose-regulated protein 78 (GRP78). The aim in the present study was to analyse the potential association between GRP78 single-nucleotide polymorphisms (SNPs) and the risk of HBV infection.


METHODS
The associations between seven common GRP78 polymorphisms in the promoter (rs391957, rs17840762, rs17840761, rs11355458) and in the 3' untranslated region (UTR) (rs16927997, rs1140763, rs12009) and possible risk of chronic HBV infection were assessed in a case-control study. 496 cases and 539 individually matched healthy controls were genotyped.


RESULTS
Overall, no associations were observed in genotypic analyses. In addition, haplotypes and diplotypes combining those SNPs in the promoter or in the 3' UTR in high linkage disequilibrium (LD) were also not associated with HBV risk.


CONCLUSIONS
These observations do not support a role for GRP78 polymorphisms in HBV infection in a predominantly Chinese Han population.
Zhu
Xiao
X
Institute of Oncology, Affiliated Tumor Hospital, Guangzhou Medical College, Guangzhou, PR China. bioxzhu@yahoo.com
Li
Dong-Pei
DP
Fan
Wen-Guo
WG
Lin
Marie C M
MC
Wang
Jin-Long
JL
Lin
Sheng-Qu
SQ
Huang
Jian-Qing
JQ
Kung
Hsiang-Fu
HF
D020413:3' Untranslated Regions; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D004198:Disease Susceptibility; D000091342:Endoplasmic Reticulum Chaperone BiP; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins; D006239:Haplotypes; D006509:Hepatitis B / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000235:genetics*; D006801:Humans; D007239:Infections / Q000235:genetics; D015810:Linkage Disequilibrium; D008565:Membrane Proteins; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D044382:Population Groups / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014780:Viruses / Q000235:genetics
D020413:3' Untranslated Regions; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; C000717247:HSPA5 protein, human; D008565:Membrane Proteins; C018551:glucose-regulated proteins