Peptide mapping by thin-layer chromatography and high voltage electrophoresis.
Thin-layer chromatography and electrophoresis, either separately or in combination, provide a simple, high resolution technique for producing peptide maps. Recently, thin-layer methods have tended to be increasingly overshadowed by the development of high performance liquid chromatography (HPLC). Although thin-layer methods are generally not as sensitive as HPLC (requiring milli- or microgram amounts of protein instead of the nanogram or less quantities used in HPLC), they are still preferred for many applications. Thin-layer peptide mapping not only has the advantage of being relatively inexpensive, but it is also a very simple technique and it is often possible to analyze a large number of different samples in a single experiment. In addition, a number of different stains can be used for the detection of specific amino acids and these considerably increase the value of this method.
MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Crewe Road, Edinburgh, UK.
K
K
Gooderham