SCORE imaging: specimen in a corrected optical rotational enclosure.
Visual data collection is paramount for the majority of scientific research. The added transparency of the zebrafish (Danio rerio) allows for a greater detail of complex biological research that accompanies seemingly simple observational tools. We developed a visual data analysis and collection approach that takes advantage of the cylindrical nature of the zebrafish allowing for an efficient and effective method for image capture that we call Specimen in a Corrected Optical Rotational Enclosure imaging. To achieve a nondistorted image, zebrafish were placed in a fluorinated ethylene propylene tube with a surrounding optically corrected imaging solution (water). By similarly matching the refractive index of the housing (fluorinated ethylene propylene tubing) to that of the inner liquid and outer liquid (water), distortion was markedly reduced, producing a crisp imagable specimen that is able to be fully rotated 360 degrees. A similar procedure was established for fixed zebrafish embryos using convenient, readily available borosilicate capillaries surrounded by 75% glycerol. The method described here could be applied to chemical genetic screening and other related high-throughput methods within the fish community and among other scientific fields.
Comparative and Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA.
Andrew M
AM
Petzold
Victoria M
VM
Bedell
Nicole J
NJ
Boczek
Jeffrey J
JJ
Essner
Darius
D
Balciunas
Karl J
KJ
Clark
Stephen C
SC
Ekker
D000715:Anatomy / Q000379:methods*; D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D005990:Glycerol; D011138:Polytetrafluoroethylene / Q000031:analogs & derivatives; D013048:Specimen Handling / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology*; D005465:Fluorocarbon Polymers
D005990:Glycerol; D011138:Polytetrafluoroethylene; C096305:fluorinated ethylene propylene; D005465:Fluorocarbon Polymers