[Study on optimizing prescription of baicalein long circulating liposomes].
OBJECTIVE
To optimize the prescription of baicalein long circulating liposomes (Baicalein LCLs).


METHODS
The liposomes were prepared by the reverse evaporating method. The entrapment efficiency and the carrying amount were determined by HPLC-Sephadex method. The main influencing factors were identified by single factor analysis, then the techniques of the prescription and preparation of liposomes were optimized by orthogonal experiment.


RESULTS
The best prescription was as follows: The proportionality of cholesterin to lecithin was 1 : 2; The dosage of PEG 4 000 was 2 mg; The proportionality of water phase to oil phase was 1 : 2; The dosage of baicalein was 30 mg.


CONCLUSIONS
After optimized, the average entrapment efficiency of liposomes is 81.42% and the average carrying amount is 32.25%.
Yan
Ren-Liang
RL
Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
Liu
Zhi-Gang
ZG
D019540:Area Under Curve; D002784:Cholesterol / Q000008:administration & dosage; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D044950:Flavanones / Q000008:administration & dosage* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D054709:Lecithins / Q000008:administration & dosage; D008081:Liposomes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000008:administration & dosage; D031364:Scutellaria baicalensis / Q000737:chemistry*
D004337:Drug Carriers; D044950:Flavanones; D054709:Lecithins; D008081:Liposomes; D011092:Polyethylene Glycols; C006680:baicalein; D002784:Cholesterol