Nano-coating of beta-galactosidase onto the surface of lactose by using an ultrasound-assisted technique.
We nano-coated powdered lactose particles with the enzyme beta-galactosidase using an ultrasound-assisted technique. Atomization of the enzyme solution did not change its activity. The amount of surface-attached beta-galactosidase was measured through its enzymatic reaction product D-galactose using a standardized method. A near-linear increase was obtained in the thickness of the enzyme coat as the treatment proceeded. Interestingly, lactose, which is a substrate for beta-galactosidase, did not undergo enzymatic degradation during processing and remained unchanged for at least 1 month. Stability of protein-coated lactose was due to the absence of water within the powder, as it was dry after the treatment procedure. In conclusion, we were able to attach the polypeptide to the core particles and determine precisely the coating efficiency of the surface-treated powder using a simple approach.
Genina
Natalja
N
Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, P.O. Box 56, 00014, Finland. natalja.genina@helsinki.fi
Räikkönen
Heikki
H
Heinämäki
Jyrki
J
Veski
Peep
P
Yliruusi
Jouko
J
D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000528:radiation effects; D004339:Drug Compounding / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000528:radiation effects; D007785:Lactose / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D013010:Sonication; D013499:Surface Properties / Q000528:radiation effects*; D001616:beta-Galactosidase / Q000737:chemistry* / Q000528:radiation effects
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D001616:beta-Galactosidase; D007785:Lactose