Isolation of salvianolic acid A, a minor phenolic carboxylic acid of Salvia miltiorrhiza.
Salvianolic acid A (Sal A), a caffeic acid derivative present in Salvia miltiorrhiza (Danshen), has shown significant biological activities, some of which are more potent than those of salvianolic acid (Sal B), the most abundant and bioactive compound in Danshen. However, its low content in the raw material makes it difficult to be used in the clinic. In this study, a novel procedure was developed to isolate Sal A from Salvia miltiorrhiza. Using this procedure, Sal A could be enriched to 1.7% in the raw material by hydrolyzing Sal B. The solution was further purified by macroporous resin and ODS column chromatography. The yield of Sal A was 0.5% with a purity of 98.0%. The procedure is efficient and convenient and is considered suitable for the separation of Sal A from Danshen.
Key Laboratory of Drug Targeting and Delivery System, Department ofPharmacognosy, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, PR China.
Luyang
L
Lu
Hao
H
Zhang
Yu
Y
Qian
Yuan
Y
Yuan
D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002681:China; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006868:Hydrolysis; D007773:Lactates / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry
D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D007773:Lactates; D045788:Sesquiterpenes, Germacrane; C530047:salviadienol B; C028972:dan-shen root extract; C066201:salvianolic acid A