Estimating prion concentration in fluids and tissues by quantitative PMCA.
Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.
Chen
Baian
B
Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School, Houston, Texas, USA.
Morales
Rodrigo
R
Barria
Marcelo A
MA
Soto
Claudio
C
D000818:Animals; D006224:Cricetinae; D008647:Mesocricetus; D018620:PrPSc Proteins / Q000097:blood* / Q000652:urine*; D017510:Protein Folding; D012608:Scrapie / Q000097:blood* / Q000652:urine*
D018620:PrPSc Proteins