Anti-CCP: challenges in quantifying auto-antibodies and creating international reference materials.
Anti-CCP assays (antibody to cyclic citrullinated peptides) possess a high specificity for RA (rheumatoid arthritis). In lack of an international reference material the absolute values among different assays strongly diversify. The cut-off showed a variance from 5 U/mL Euroimmun anti-CCP to 25 U/mL Euro-Diagnostica Immunosan RA. The Autoantibody Standardization Committee is currently trying to establish a reference specimen of human polyclonal anti-CCP.
Rottmann
Michael
M
Roche Diagnostics, Penzberg, Germany. michael.rottmann@roche.com
D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards*; D007391:International Cooperation; D010456:Peptides, Cyclic / Q000276:immunology*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards
D001323:Autoantibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide