Light induced degradation of testosterone in waters.
The degradation of testosterone under simulated irradiations was studied in phosphate buffers and in natural waters at various excitation wavelengths. The quantum yield of photolysis was significantly lower at 313 nm (2.4 x 10(-3)) than at 254 nm (0.225). The formation of several photoproducts was observed, some of them being rapidly transformed in turn while others show higher stability towards subsequent irradiations. The nature of the main products was tentatively identified, both deduced from their spectral and spectrometric data and by comparison with synthesised standard compounds. Among the obtained photoproducts, the main one is possibly a spiro-compound, hydroxylated derivative of testosterone originating from the photohydratation of the enone group. The photodegradation pathway includes also photorearrangements. One of them leads to (1,5,10)-cyclopropyl-17beta-hydroxyandrostane-2-one. The pH of the water does not seem to affect the rate of phototransformation and the nature of the by-products.
Vulliet
Emmanuelle
E
Service Central d'Analyse du CNRS-USR59, Chemin du Canal, F-69360 Solaize, France. e.vulliet@sca.cnrs.fr
Falletta
Marine
M
Marote
Pedro
P
Lomberget
Thierry
T
Païssé
Jean-Olivier
JO
Grenier-Loustalot
Marie-Florence
MF
D052918:Environmental Restoration and Remediation; D010782:Photolysis; D013739:Testosterone / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects
D014874:Water Pollutants, Chemical; D014867:Water; D013739:Testosterone