Molecular characterization and epidemiology of rotavirus isolates obtained from children with diarrhoea in Malaysia.
This retrospective study examined the G/P type of rotavirus in RNA samples that have previously been e-typed by RNA-PAGE in 1996. The results were then compared to 2007 samples to ascertain the extent of changes that may have occurred in this 11-years time interval. The G and P genotypes were determined by hemi-nested PCR and further analysed by phylogenetic study. In 1996, the G/P combination G1P[8], G(UT)P[8] and G1P(UT) prevalence rate were 81%, 9% and 7%, respectively. As expected, the G9 genotype which has already emerged worldwide was identified in 42% of the 2007 samples with the remaining 33% G1P[8] and 25% G1P(UT) Analysis of the RNA pattern showed that majority of the isolates were long e-type in both series, nevertheless minor differences within electropherotypes were observed. Genetic diversity in some strains of the human group A rotaviruses was analysed by phylogenetic methods. These findings will help in the decision to introduce rotavirus vaccines within the next decade.
Faculty of Health Sciences, Universiti Teknologi MARA, Jalan Othman, 46000 Petaling Jaya, Selangor, Malaysia. drzuridah@salam.uitm.edu.my
H
H
Zuridah
C D
CD
Kirkwood
R F
RF
Bishop
N
N
Bogdanovic-Sakran
K L
KL
Yap
D002648:Child; D003967:Diarrhea / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology*; D013997:Time Factors
D012367:RNA, Viral