Carbon nanotube composites for glucose biosensor incorporated with reverse iontophoresis function for noninvasive glucose monitoring.
This study aims to develop an amperometric glucose biosensor, based on carbon nanotubes material for reverse iontophoresis, fabricated by immobilizing a mixture of glucose oxidase (GOD) and multiwalled carbon nanotubes (MWCNT) epoxy-composite, on a planar screen-printed carbon electrode. MWCNT was employed to ensure proper incorporation into the epoxy mixture and faster electron transfer between the GOD and the transducer. Results showed this biosensor possesses a low detection potential (+500 mV), good sensitivity (4 microA/mM) and an excellent linear response range (r(2) = 0.999; 0-4 mM) of glucose detection at +500 mV (versus Ag/AgCl). The response time of the biosensor was about 25 s. In addition, the biosensor could be used in conjunction with reverse iontophoresis technique. In an actual evaluation model, an excellent linear relationship (r(2) = 0.986) was found between the glucose concentration of the actual model and the biosensor's current response. Thus, a glucose biosensor based on carbon nanotube composites and incorporated with reverse iontophoresis function was developed.
Department of Electrical Engineering, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou,Taiwan, ROC.
Tai-Ping
TP
Sun
Hsiu-Li
HL
Shieh
Congo Tak-Shing
CT
Ching
Yan-Dong
YD
Yao
Su-Hua
SH
Huang
Chia-Ming
CM
Liu
Wei-Hao
WH
Liu
Chung-Yuan
CY
Chen
D015374:Biosensing Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D007478:Iontophoresis / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D037742:Nanotubes, Carbon / Q000737:chemistry*
D037742:Nanotubes, Carbon; D005947:Glucose