ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 as a subunit of this meta-coactivator complex (MECO). Finally, we demonstrate that the MECO regulates a subset of RNA polymerase II-transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable subcomplexes to facilitate their combined actions on specific target genes.
Krebs
Arnaud R
AR
Department of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Parc d'Innovation, 1 rue Laurent Fries, CNRS UMR 7104, INSERM U 964, Université de Strasbourg, BP 10142, Illkirch Cedex 67404, France.
Demmers
Jeroen
J
Karmodiya
Krishanpal
K
Chang
Nan-Chi
NC
Chang
Alice Chien
AC
Tora
László
L
D000818:Animals; D002478:Cells, Cultured; D019744:Chemokines, C / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D016350:Leucine Zippers; D013058:Mass Spectrometry; D056892:Mediator Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D046912:Multiprotein Complexes / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics*
D019744:Chemokines, C; D004268:DNA-Binding Proteins; C103593:Luzp1 protein, mouse; D056892:Mediator Complex; D046912:Multiprotein Complexes; D010455:Peptides; D021122:Protein Subunits; D022661:RNA, Untranslated; C486249:Xcl1 protein, mouse