S. pombe genome deletion project: an update.
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.
Spirek
Mario
M
Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Benko
Zsigmond
Z
Carnecka
Martina
M
Rumpf
Cornelia
C
Cipak
Lubos
L
Batova
Monika
M
Marova
Ivana
I
Nam
Miyoung
M
Kim
Dong-Uk
DU
Park
Han-Oh
HO
Hayles
Jacqueline
J
Hoe
Kwang-Lae
KL
Nurse
Paul
P
Gregan
Juraj
J
D004262:DNA Restriction Enzymes; D017353:Gene Deletion; D055786:Gene Knockout Techniques / Q000379:methods*; D005822:Genetic Vectors; D016681:Genome, Fungal; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics*
D004262:DNA Restriction Enzymes