Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic.
During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had > or =50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42, Brno, Czech Republic. bartovae@vfu.cz
Eva
E
Bártová
Kamil
K
Sedlák
Michaela
M
Syrová
Ivan
I
Literák
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D003048:Coccidiosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D018153:Czech Republic / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006734:Horse Diseases / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology; D006736:Horses; D007841:Latex Fixation Tests; D018688:Neospora / Q000276:immunology*; D016036:Seroepidemiologic Studies; D044967:Serum / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000276:immunology
D000913:Antibodies, Protozoan