Enhancement in the efficiency of polymerase chain reaction by TiO2 nanoparticles: crucial role of enhanced thermal conductivity.
Improvement of the specificity and efficiency of the polymerase chain reaction (PCR) by nanoparticles is an emerging area of research. We observed that TiO(2) nanoparticles of approximately 25 nm diameter caused significant enhancement of PCR efficiency for various types of templates (namely plasmid DNA, genomic DNA and complementary DNA). By a series of experiments, the optimal TiO(2) concentration was determined to be 0.4 nM, which resulted in up to a seven-fold increase in the amount of PCR product. As much as 50% reduction in overall reaction time (by reduction of the number of cycles and the time periods of cycles) was also achieved by utilizing TiO(2) nanoparticles without compromising the PCR yield. Investigations of the mechanism of such PCR enhancement by simulations using the 'Fluent K epsilon turbulent model' provided evidence of faster heat transfer in the presence of TiO(2) nanoparticles. Consistent with these findings, TiO(2) nanoparticles were observed to augment the denaturation of genomic DNA, indicating more efficient thermal conductivity through the reaction buffer. TiO(2) nanoparticle-assisted PCR may be useful for profound reduction of the overall PCR reaction period and for enhanced amplification of DNA amplicons from a variety of samples, including GC-rich templates that are often observed to yield unsatisfactory results.
Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India.
R
R
Abdul Khaliq
Parshuram J
PJ
Sonawane
Binu K
BK
Sasi
Bhavani S
BS
Sahu
T
T
Pradeep
Sarit K
SK
Das
Nitish R
NR
Mahapatra
D000818:Animals; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D004247:DNA; D014025:Titanium