Comparative expression analysis of rpf-like genes of Mycobacterium tuberculosis H37Rv under different physiological stress and growth conditions.
Mycobacterium tuberculosis H37Rv possesses five resuscitation-promoting factors, RpfA-E, which are required for the resuscitation of dormancy in mycobacteria induced by prolonged incubation of the culture in stationary phase. This study explores the transcriptional profile of all the rpf-like genes of M. tuberculosis H37Rv in the exponential phase, stationary phase, non-culturable phase and Rpf-mediated resuscitation phase. The relative expression was also monitored under acid stress, nutrient starvation and low-oxygen (hypoxia) conditions by real-time quantitative PCR. We show differential relative expression of the rpf genes during different stages of growth and under stress. During early resuscitation, all rpf genes were expressed with maximal expression ratios for rpfA and rpfD. rpfC was consistently expressed during all stages of growth and nutrient starvation. Acid stress induced higher relative expression of rpfD and rpfE and hypoxia of rpfC and rpfE. These results therefore provide further evidence that the rpf genes perform distinct roles during cell growth and cell survival under different physiological stresses, and are consistent with the rpf-like genes being differentially regulated.
Microbiology Division, Central Drug Research Institute, CSIR, Lucknow 226001, India.
Ravi Kr
RK
Gupta
Microbiology Division, Central Drug Research Institute, CSIR, Lucknow 226001, India.
Brahm S
BS
Srivastava
Microbiology Division, Central Drug Research Institute, CSIR, Lucknow 226001, India.
Ranjana
R
Srivastava
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D013312:Stress, Physiological
D001426:Bacterial Proteins