A real-time SUMO-binding assay for the analysis of the SUMO-SIM protein interaction network.
SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.
Tanaka
Niina
N
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
Saitoh
Hisato
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011496:Protein Methyltransferases / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins; D025842:SUMO-1 Protein / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
C501309:ATF7IP protein, human; D012097:Repressor Proteins; D025842:SUMO-1 Protein; D014157:Transcription Factors; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C454062:SETDB1 protein, human