A rapid method with ultra-high-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines.
A speedy and selective ultra-HPLC-MS/MS method for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one-step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile-phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 microm) were employed to obtain the best resolution of the target analytes. [(13)C(15)]-DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity (R(2) > or = 0.9990), sensitivity (LOQ, 0.29-0.99 microg/kg), recovery (88.5-119.5%) and precision (RSD < or = 15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3-ADON, 15-ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.
Han
Zheng
Z
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
Liu
Xuesong
X
Ren
Yiping
Y
Luan
Lianjun
L
Wu
Yongjiang
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D008968:Molecular Conformation; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014255:Trichothecenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D014255:Trichothecenes; C002469:fusarenon-X; C043247:3-acetyldeoxynivalenol; C038405:nivalenol; C046760:15-acetyldeoxynivalenol; C007262:deoxynivalenol