Hydroxyurea-induced global transcriptional suppression in mouse ES cells.
Hydroxyurea (HU), as a therapeutic medicine, has been extensively used clinically. To further survey molecular mechanisms of HU treatment, we analyzed global transcriptomic alteration of mouse ES cells in response to the treatment using high-throughput sequencing. We show that the global transcriptional activity is significantly suppressed as cells are exposed to HU treatment and alters multiple key cellular pathways, including cell cycle, apoptosis and DNAs. HU treatment also alters alternative splicing mechanisms and suppresses non-coding RNA expression. Our result provides novel clues for the understanding of how cells respond to HU and further suggests that high-throughput sequencing technology provides a powerful tool to study mechanisms of clinical drugs at the cellular level.
Cui
Peng
P
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang, 100029 Beijing, China.
Lin
Qiang
Q
Xin
Chengqi
C
Han
Lu
L
An
Lili
L
Wang
Yulan
Y
Hu
Zhishang
Z
Ding
Feng
F
Zhang
Lingfang
L
Hu
Songnian
S
Hang
Haiying
H
Yu
Jun
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000187:drug effects*; D020869:Gene Expression Profiling; D006918:Hydroxyurea / Q000494:pharmacology*; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
D014408:Biomarkers, Tumor; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D006918:Hydroxyurea