Simultaneous determination of yohimbine, sildenafil, vardenafil and tadalafil in dietary supplements using high-performance liquid chromatography-tandem mass spectrometry.
A simple and sensitive method was developed for determination of illegal adulterants (yohimbine, sildenafil, vardenafil and tadalafil) in dietary supplements by HPLC-MS/MS. The separation was achieved on a C(18) column with the mobile phase consisting of acetonitrile and 0.1% acetic acid aqueous solution with a gradient elution at a flow rate of 0.5 mL/min. The analytes were quantified and identified by two characteristic transitions using the multiple-reaction monitoring mode. The recoveries of the analytes ranged from 77.5 to 109.3% with the RSD less than 8.1% (n=6). The method has been successfully applied to screen illegal adulterations of natural dietary supplements.
Hunan Academy of Inspection & Quarantine, Hunan Key Lab. of Science & Technology for Food Safety, Technogy Center of Hunan Entry-Exit Inspection & Quarantine Bureau of P. R. China, Changsha, P. R. China.
Ying
Y
Zhang
Zhiqiang
Z
Huang
Li
L
Ding
Hongfei
H
Yan
Meiling
M
Wang
Shaohua
S
Zhu
D000317:Adrenergic alpha-Antagonists / Q000032:analysis / Q000627:therapeutic use; D002243:Carbolines / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D019587:Dietary Supplements / Q000032:analysis*; D007172:Erectile Dysfunction / Q000188:drug therapy; D006801:Humans; D007093:Imidazoles / Q000032:analysis* / Q000627:therapeutic use; D008297:Male; D015394:Molecular Structure; D010726:Phosphodiesterase Inhibitors / Q000032:analysis / Q000627:therapeutic use; D010879:Piperazines / Q000032:analysis* / Q000627:therapeutic use; D011687:Purines / Q000032:analysis / Q000627:therapeutic use; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000032:analysis* / Q000627:therapeutic use; D000068581:Tadalafil; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014227:Triazines / Q000032:analysis / Q000627:therapeutic use; D000069058:Vardenafil Dihydrochloride; D015016:Yohimbine / Q000032:analysis* / Q000627:therapeutic use
D000317:Adrenergic alpha-Antagonists; D002243:Carbolines; D007093:Imidazoles; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D014227:Triazines; D015016:Yohimbine; D000069058:Vardenafil Dihydrochloride; D000068581:Tadalafil; D000068677:Sildenafil Citrate