Deregulation of miR-92a expression is implicated in hepatocellular carcinoma development.
MicroRNAs (miRNAs) belong to a class of the endogenously expressed non-coding small RNAs which primarily function as gene regulators. Growing evidence suggests that miRNAs have a significant role in tumor development and may constitute robust biomarkers for cancer diagnosis and prognosis. The miR-17-92 cluster especially is markedly overexpressed in several cancers, and is associated with the cancer development and progression. In this study, we have demonstrated that miR-92a is highly expressed in hepatocellular carcinoma (HCC). In addition, the proliferation of HCC-derived cell lines was enhanced by miR-92a and inhibited by the anti-miR-92a antagomir. On the other hand, we have found that the relative amount of miR-92a in the plasmas from HCC patients is decreased compared with that from the healthy donors. Interestingly, the amount of miR-92a was elevated after surgical treatment. Thus, although the physiological significance of the decrease of miR-92a in plasma is still unknown, deregulation of miR-92 expression in cells and plasma should be implicated in the development of HCC.
Third Department of Surgery, Tokyo Medical University, Tokyo, Japan.
Masatoshi
M
Shigoka
Akihiko
A
Tsuchida
Takaaki
T
Matsudo
Yuichi
Y
Nagakawa
Hitoshi
H
Saito
Yoshiaki
Y
Suzuki
Tatsuya
T
Aoki
Yoshiki
Y
Murakami
Hidenori
H
Toyoda
Takashi
T
Kumada
Ralf
R
Bartenschlager
Nobuyuki
N
Kato
Masanori
M
Ikeda
Tomoki
T
Takashina
Masami
M
Tanaka
Rieko
R
Suzuki
Kosuke
K
Oikawa
Masakatsu
M
Takanashi
Masahiko
M
Kuroda
D000328:Adult; D000368:Aged; D000070416:Antagomirs; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D017403:In Situ Hybridization; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009843:Oligoribonucleotides / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D000070416:Antagomirs; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D009843:Oligoribonucleotides; C541876:antagomir-92a