Immune responses elicited in mice with recombinant Lactococcus lactis expressing F4 fimbrial adhesin FaeG by oral immunization.
Enterotoxigenic Escherichia coli (ETEC) is a major pathogenic agent causing piglet diarrhea. The major subunit and adhesin FaeG of F4(+) ETEC is an important virulence factor with strong immunogenicity. To determine whether Lactococcus lactis (L. lactis) could effectively deliver FaeG to the mucosal immune system, recombinant L. lactis expressing FaeG was constructed, and immune responses in mice following oral route delivery of recombinant L. lactis were explored. The production of FaeG expressed in L. lactis was up to approximately 10% of soluble whole-cell proteins, and recombinant FaeG (rFaeG) possessed good immunoreactivity by Western blot analysis. Oral immunization with recombinant L. lactis expressing FaeG induced F4-specific mucosal and systemic immune responses in the mice. In addition, high dose recombinant L. lactis or co-administration of high dose recombinant L. lactis with CTB enhanced the immune responses. These results suggested that L. lactis expressing FaeG was a promising candidate vaccine against ETEC.
State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Shujie
S
Liu
Yongming
Y
Li
Ziwei
Z
Xu
Yicheng
Y
Wang
D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000284:Administration, Oral; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005260:Female; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013047:Specific Pathogen-Free Organisms
D018830:Adhesins, Escherichia coli; D001428:Bacterial Vaccines; C494388:FaeG protein, E coli; D007070:Immunoglobulin A; D007074:Immunoglobulin G