RBC membrane damage and decreased band 3 phospho-tyrosine phosphatase activity are markers of COPD progression.
Injury to red blood cell (RBC) membrane by oxidative stress is of clinical importance in chronic obstructive pulmonary disease (COPD) which leads to oxidative stress (OE) during disease progression. Here, we studied the impact of this stress on injury to RBC membrane. Blood samples from both healthy volunteers (HV, n = 11) and controlled COPD patients (n=43) were divided according to their GOLD disease stage (I=7, II=21, III=10, IV=5). Plasma levels of paraoxonase (PON) activity, protein carbonyls (PC), conjugate dienes, lipohydroperoxides (LPH) and malondialdehyde (MDA) were determined and the PTPase, and the oxidative parameters were measured in RBC ghosts. Plasma from patients with COPD showed an increased oxidation of lipids and proteins, that correlated with the disease progression. PON activity decreased from GOLD stages II to IV and correlated with an increase in LPH (p less than 0.0001, r = -0.8115). There was evidence of an increase in the oxidative biomarkers in RBCs, while the PTPase activity was diminished in stage III and IV of COPD. In conclusion, OE-induced injury associated with COPD is associated with an oxidative damage to the RBC membrane, with a concomitant decrease in the PTPase activity and altered function of anionic exchanger (AE1).
Torres-Ramos
Yessica Dorin
YD
Departamento de Bioquimica y Biologia Molecular del Instituto Nacional de Perinatologia, Isidro Espinosa de los Reyes, Mexico.
Guzman-Grenfell
Alberto Martin
AM
Montoya-Estrada
Araceli
A
Ramirez-Venegas
Alejandra
A
Martinez
Raul Sansores
RS
Flores-Trujillo
Fernando
F
Ochoa-Cautino
Leticia
L
Hicks
Juan Jose
JJ
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood*; D018450:Disease Progression; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000473:pathology
D001457:Anion Exchange Protein 1, Erythrocyte; D015415:Biomarkers; D017027:Protein Tyrosine Phosphatases