Introduction of new tools for chemical biology research on microbial metabolites.
Recently, high-throughput screening (HTS) has become the mainstream technique for drug discovery. Compounds that are synthesized by combinatorial chemistry might be more suitable than natural products to apply to HTS, because the purification procedure is a drawback of using natural products. Nevertheless, natural products remain an extremely important source of drugs. To overcome the demerits of natural products, we are constructing the RIKEN Natural Products Depository (NPDepo) that is focused primarily on microbial metabolites. In this review, I describe (i) engineering pathways for biosynthetic gene clusters of microbial metabolites, (ii) construction of fraction libraries of microbial metabolites, and (iii) the development of a new screening system using a chemical array and a protein library produced by GLORIA.
Chemical Biology Department, RIKEN Advanced Science Institute, Saitama, Japan. hisyo@riken.jp
Hiroyuki
H
Osada
D000595:Amino Acid Sequence; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D002621:Chemistry; D055808:Drug Discovery / Q000295:instrumentation / Q000379:methods*; D015723:Gene Library; D005818:Genetic Engineering; D006801:Humans; D008829:Microbiology; D008969:Molecular Sequence Data
D001688:Biological Products