A simple fluorescent strategy for in situ evaluation of cell surface carbohydrate with a quantum dot-lectin nanoprobe.
A simple and rapid fluorescent method was developed for the in situ evaluation of cell surface carbohydrate by homogeneous specific recognition of a quantum dot-lectin nanoprobe to mannosyl groups on the cell surface. The strategy was further used for dynamically monitoring the alteration of cell surface carbohydrate expression in response to drugs.
Xu
Xu
X
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
Ding
Lin
L
Xue
Yadong
Y
Ju
Huangxian
H
D019187:Cadmium Compounds / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D053758:Nanoparticles / Q000737:chemistry*; D011422:Propionates / Q000737:chemistry; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D002241:Carbohydrates; D005456:Fluorescent Dyes; D011422:Propionates; D003208:Concanavalin A; D013691:Tellurium; C028337:cadmium telluride