The binding of corticosterone to the class-theta  glutathione S-transferase from the eyes of the  shrimp Penaeus japonicus (Crustacea: Decapoda).
The anionic homodimeric theta glutathione S-transferase (EC 2.5.1.18), with specific activities of 4.6 units/mg with 1-chloro-2,4-dinitrobenzene as substrate, from the eyes of the shrimp Penaeus japonicus, binds to corticosterone, which was confirmed by the analysis of the conjugate by SDS-PAGE and fluorography of proteins with bound (3)H-corticosterone.The reaction of the theta glutathione S-transferase with corticosterone was prevented by the presence of glutathione at 10 mM.
Division of Biochemistry and Molecular Science, Institute of Zoology, Academia Sinica,Nankang, Taipei, Taiwan, Republic of China.
T
T
Shu-Fen
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Chuang
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003345:Corticosterone / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005123:Eye / Q000201:enzymology; D005978:Glutathione / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism*; D033561:Penaeidae / Q000201:enzymology*; D013997:Time Factors
D004791:Enzyme Inhibitors; D005982:Glutathione Transferase; D005978:Glutathione; D003345:Corticosterone