Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging.
FTIR microscopy with a focal plane array (FPA) of detectors enables routine chemical imaging on individual cells in only a few minutes. The brilliance of synchrotron radiation (SR) IR sources may enhance the signal obtained from such small biosamples containing small amounts of organic matter. We investigated individual cells obtained from a cell culture specifically developed for transmission FTIR imaging using either a Globar or an SR source coupled to the same instrumentation. SR-IR source focussing was optimized to control the energy distribution on the FPA of detectors. Here we show that accessing the IR absorption distribution from all the organic contents of cells at 1 x 1 microm pixel resolution was possible only with high circulating current (> or = 1.2 A) illuminating a limited number of the FPA's detectors to increase the signal-to-noise ratio of IR images. Finally, a high-current SR ring is mandatory for collecting FTIR images of biosamples with a high contrast in minutes.
Université de Bordeaux 2, CNRS UMR 5084, B8 Avenue des Facultés, 33405 Pessac-Cedex, France. cyril.petibois@u-bordeaux2.fr
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Petibois
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Cestelli-Guidi
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Piccinini
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Moenner
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Marcelli
D002460:Cell Line; D002477:Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D022082:Cellular Structures / Q000737:chemistry / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009930:Organic Chemicals / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods; D017356:Synchrotrons
D009930:Organic Chemicals