Focal modulation microscopy: a theoretical study.
Focal modulation microscopy is an emerging fluorescence microscopy technique for in vivo imaging of thick biological tissues. Here, we present a theoretical study to assess its performance. The scalar diffraction theory is combined with Monte Carlo simulation to evaluate the signal-to-background ratio at various depths. The performance of confocal microscopy with a similar optical setup is also evaluated for comparison.
Chong
Shau Poh
SP
Division of Bioengineering, National University of Singapore, 9 Engineering Drive 1, #EA-03-12, Singapore 129789.
Wong
Chee Howe
CH
Sheppard
Colin J R
CJ
Chen
Nanguang
N
D000465:Algorithms; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity