Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.
The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.
Gundry
Rebekah L
RL
Department of Medicine, The Johns Hopkins University, Baltimore, MD 21224, USA.
Tchernyshyov
Irina
I
Sheng
Shijun
S
Tarasova
Yelena
Y
Raginski
Kimberly
K
Boheler
Kenneth R
KR
Van Eyk
Jennifer E
JE
D000818:Animals; D002460:Cell Line; D030562:Databases, Protein; D053595:Embryonic Stem Cells / Q000378:metabolism*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D020543:Proteome