Characterization of the adhesive properties of the type IIb subfamily receptor protein tyrosine phosphatases.
Receptor protein tyrosine phosphatases (RPTPs) have cell adhesion molecule-like extracellular domains coupled to cytoplasmic tyrosine phosphatase domains. PTPmu is the prototypical member of the type IIb subfamily of RPTPs, which includes PTPrho, PTPkappa, and PCP-2. The authors performed the first comprehensive analysis of the subfamily in one system, examining adhesion and antibody recognition. The authors evaluated if antibodies that they developed to detect PTPmu also recognized other subfamily members. Notably, each antibody recognizes distinct subsets of type IIb RPTPs. PTPmu, PTPrho, and PTPkappa have all been shown to mediate cell-cell aggregation, and prior work with PCP-2 indicated that it can mediate bead aggregation in vitro. This study reveals that PCP-2 is unique among the type IIb RPTPs in that it does not mediate cell-cell aggregation via homophilic binding. The authors conclude from these experiments that PCP-2 is likely to have a distinct biological function other than cell-cell aggregation.
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, USA.
Scott
S
Becka
Peng
P
Zhang
Sonya E L
SE
Craig
David T
DT
Lodowski
Zhenghe
Z
Wang
Susann M
SM
Brady-Kalnay
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D002448:Cell Adhesion; D002460:Cell Line; D003429:Cross Reactions; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D018411:Spodoptera / Q000166:cytology; D055672:Static Electricity
D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2