Molecular cloning, genomic structure, and genetic mapping of two Rdl-orthologous genes of GABA receptors in the diamondback moth, Plutella xylostella.
The Resistance to dieldrin (Rdl) gene encodes a subunit of the insect gamma-aminobutyric acid (GABA) receptor. Cyclodiene resistance in many insects is associated with replacement of a single amino acid (alanine at position 302) with either a serine or a glycine in the Rdl gene. Two Rdl-orthologous genes of GABA receptors (PxGABARalpha1 and PxGABARalpha2) were cloned and sequenced from a susceptible strain (Roth) of Plutella xylostella. PxGABARalpha1 and PxGABARalpha2 showed 84% and 77% identity with the Rdl gene of Drosophila melanogaster at an amino acid level, respectively. The coding regions of PxGABARalpha1 and PxGABARalpha2 both comprise ten exons, with two alternative RNA-splicing forms in exon 3 of both genes. At the orthologous position of alanine-302 in D. melanogaster Rdl, PxGABARalpha1 has a conserved alanine at position 282. PxGABARalpha2 has a serine instead of an alanine at the equivalent position. With two informative DNA markers, both PxGABARalpha1 and PxGABARalpha2 were mapped onto the Z chromosome of P. xylostella.
Yuan
Guorui
G
College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects (Ministry of Agriculture of China), Nanjing, China.
Gao
Weiyue
W
Yang
Yihua
Y
Wu
Yidong
Y
D017398:Alternative Splicing / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005819:Genetic Markers; D023281:Genomics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018079:Receptors, GABA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D005819:Genetic Markers; D007306:Insecticides; D012333:RNA, Messenger; D018079:Receptors, GABA