Effect of oxidized fibrinogen on hemostatic system: in vitro study.
Standard coagulation assays were performed with control and oxidized fibrinogen (Fg), using prothrombin time (PT; 12.5 ± 0.4 vs 25 ± 0.8 seconds, P < .001) and activated partial thromboplastin time (aPTT; 33 ± 2.5 vs 63 ± 4.7 seconds, P < .001). Fibrin clot (MA), clot formation initiation (r), and rate of clot lysis (LY30) were measured, a reflection exposure of Fg to Fe(3+)/ ascorbate oxidative system by thrombelastograph (TEG) analysis (0, 6, 12, 24, and 48 hours, 6.2 ± 1.3 vs 5.5 ± 1.2, 4.3 ± 1.0 [P < .01], 3.9 ± 1.6, 3.2 ± 0.8, [P < .001]). Maximum amplitude level was found to be lower than control (69.1 ± 7.2 vs 67.9 ± 12.4, 64.0 ± 11.4, 60.2 ± 21.2, 42.2 ± 15.2, P < .001). The lysis rate was changed according to oxidation time between Fg exposed to Fe(3+)/ascorbate and control exposed to Fe( 3+)/ascorbate for the same treatment time (1.9 ± 0.71 vs 7 ± 0.5, 1.6 ± 0.1, 1.2 ± 0.5, 0.9 ± 1.3, P < .001). We revealed dysregulation of hemostatic system with contribution of oxidized Fg, which was in direct proportion to the intensity of Fg oxidation.
Department of Biochemistry, Faculty of Pharmacy, Marmara University, Istanbul, Turkey. stetik@marmara.edu.tr
Sermin
S
Tetik
Kurtulus
K
Kaya
Turay
T
Yardimci
D000328:Adult; D001780:Blood Coagulation Tests / Q000379:methods; D005260:Female; D005340:Fibrinogen; D006487:Hemostasis; D006489:Hemostatic Techniques / Q000295:instrumentation*; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D013997:Time Factors
D005340:Fibrinogen