Rhizobium kunmingense sp. nov., isolated from rhizosphere soil of Camptotheca acuminata Decne.
Strain LXD30(T) was isolated from rhizosphere soil of a plant of the species Camptotheca acuminata Decne which is native to warm, humid stream banks in southern China. Analysis of the 16S rRNA gene sequence revealed that the Gram-negative, rod-shaped bacterium fell within the realm of the genus Rhizobium and was most closely related to Rhizobium huautlense SO2(T) (96.4% sequence similarity) and Rhizobium cellulosilyticum LMG 23642(T) (96.4%). The isolate grew optimally at pH7.0 and 25-28 degrees C in the presence of 0-1% (w/v) NaCl. Major fatty acids were C16:0 (17.5%) and summed feature 7 (C18:1omega7c/omega9t/omega12t, 58.3%). Unequivocally low 16S rRNA (<97%), recA (<92%) and atpD (<90%) gene sequence similarities to all existing species of the genus and phenotypic characteristics all suggested that strain LXD30(T) (=KCTC 22609(T)=CGMCC 1.8903(T)) represents a novel Rhizobium species, for which the name Rhizobium kunmingense sp. nov. is proposed.
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
Lei
L
Shen
Long-Peng
LP
Zheng
Huan
H
Liu
Rui
R
Liu
Ke-Yun
KY
Zhang
Ren
R
Lai
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D029769:Camptotheca / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S