Development of succinic acid production from corncob hydrolysate by Actinobacillus succinogenes.
Succinic acid is one of the most important platform chemicals since it has great potential in industrial applications. In this study, corncob hydrolysate was used for succinic acid production. After diluted acid treatment, xylose was released from hemicellulose as the predominant monosaccharide in the hydrolysate, whereas glucose was released very little and most was retained as cellulose in the raw material. Without any detoxification, corncob hydrolysate was used directly as the carbon source in the fermentation. Actinobacillus succinogenes could utilize the sugars in the hydrolysate to produce succinic acid efficiently. Through medium optimization, yeast extract was selected as the nitrogen source and MgCO3 was used to control pH. A total of 23.64 g/l of succinic acid was produced with a yield of 0.58 g/g based on consumed sugar, indicating that the waste corncob residue can be used to produce value-added chemicals practically.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Jie
J
Yu
Zhimin
Z
Li
Qin
Q
Ye
Yong
Y
Yang
Shulin
S
Chen
D000188:Actinobacillus / Q000378:metabolism*; D000383:Agriculture; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007220:Industrial Waste; D010461:Peptones / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
D003470:Culture Media; D007220:Industrial Waste; D010461:Peptones; D011134:Polysaccharides; C007916:hemicellulose; D014994:Xylose; D019802:Succinic Acid; D005947:Glucose