Crystallization and preliminary crystallographic studies of CorC, a magnesium-ion transporter.
CorC is a magnesium transporter that is involved in the Mg(2+)-efflux function of the CorA transporter system, an Mg(2+) channel, from Shigella flexneri. Native CorC was purified and crystallized in the native form and in a ligand-free form and diffraction data sets were collected to 2.9 and 3.4 A resolution, respectively. The native CorC crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.31, b = 74.44, c = 132.78 A. The ligand-free CorC crystals belonged to space group P3(1)21/P3(2)21, with unit-cell parameters a = b = 71.89, c = 125.96 A. The CorC-ATP complex has also been crystallized and the crystals belonged to space group P2 or P2(1).
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
Ning
N
Zhang
Xiaoming
X
Ren
Deyu
D
Zhu
Defeng
D
Li
Dacheng
D
Wang
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D027682:Cation Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D011485:Protein Binding
D027682:Cation Transport Proteins; D000255:Adenosine Triphosphate