ESI-MS studies of silver ion competitive interaction with cysteine-containing peptides and sulfur-containing amino aсids.
The paper deals with investigation of silver ion interaction with sulfur-bearing amino acids and cysteine-bearing peptides using an electrospray ionisation orthogonal ion introduction time-of-flight (ESI-o-TOF) mass spectrometer. It has been shown that Cys and Hcy demonstrate the largest affinity for silver, both in relation to methionine and cysteine residues in peptides. The studies have for the first time revealed the effect of predominant forming of ions containing two silver atoms per a sulfhydryl group of cysteine-bearing peptides if the nearest microenvironment does not hinder this.
Institute for Analytical Instrumentation, RAS, St. Petersburg, Russia.
A V
AV
Novikov
R A
RA
Bublyaev
N V
NV
Krasnov
Yu P
YP
Kozmin
O A
OA
Mirgorodskaya
D000603:Amino Acids, Sulfur / Q000737:chemistry* / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D000603:Amino Acids, Sulfur; D010455:Peptides; D012834:Silver; D014357:Trypsin; D003545:Cysteine