[The establishment of a rapid fluorescent focus inhibition test for testing rabies virus neutralizing antibody].
OBJECTIVE
To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody. CVS-11 was used as the standard challenge virus, and three generations prepared for the establishment of the virus library.


METHODS
International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated.


RESULTS
We established the RFFIT which showed both good specificity (100%) and reproducibility (P > 0.5).


CONCLUSIONS
The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.
Yu
Peng-cheng
PC
Animal Science and Technology College, Qingdao Agriculture University, Qingdao 266109, China.
Lv
Xin-jun
XJ
Shen
Xin-xin
XX
Cao
Lei
L
Zheng
Xin-xiong
XX
Shan
Hu
H
Tang
Qing
Q
D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D009500:Neutralization Tests / Q000379:methods; D011820:Rabies virus / Q000276:immunology / Q000302:isolation & purification*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral