Novel angiotensin-converting enzyme (ACE) inhibitory peptides derived from boneless chicken leg meat.
Four peptides that inhibit angiotensin-converting enzyme (ACE) were separated from the hydorlysate of boneless chicken leg meat digested with artificial gastric juice (pepsin). Two peptides were identified as the peptides encrypted in myosin heavy chain. The peptide P1 (MNVKHWPWMK) corresponds to the amino acid sequence from amino acids 825 to 834 of myosin heavy chain, and the peptide P4 (VTVNPYKWLP) corresponds to the amino acid sequence from amino acids 125 to 135 of myosin heavy chain. They are novel ACE inhibitory peptides derived from chicken, and IC(50) values of P1 and P4 were determined as 228 and 5.5 microM, respectively. Although these values were much larger than 0.022 microM for captopril, a typical synthetic ACE inhibitor, they are comparable to IC(50) values reported for various ACE inhibitory peptides derived from foods. Because the peptide P4 has a relatively low IC(50) value, it is a good starting substance for designing food supplements for hypertensive patients.
Terashima
Masaaki
M
Department of Biosphere Sciences, School of Human Sciences, Kobe College 4-1, Nishinomiya City, Hyogo, Japan.
Baba
Takako
T
Ikemoto
Narumi
N
Katayama
Midori
M
Morimoto
Tomoko
T
Matsumura
Saki
S
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D006614:Hindlimb / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000032:analysis
D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D007703:Peptidyl-Dipeptidase A