A fish-oil-rich diet reduces vascular oxidative stress in apoE(-/-) mice.
Oxidative stress contributes to lipid peroxidation and decreases nitric oxide (NO) bioavailability in atherosclerosis. While long-chain (n-3) polyunsaturated fatty acids (PUFA) are easily oxidized in vitro, they improve endothelial function. Hence, this study postulates that long-chain (n-3) PUFA decrease atherogenic oxidative stress in vivo. To test this, apoE(-/-) mice were fed a corn oil- or a fish oil (FO)-rich diet for 8, 14 or 20 weeks and parameters related to NO and superoxide (O(2)(.-)) plus markers of lipid peroxidation and protein oxidative damage in the aortic root were evaluated. The FO-rich diet increased NO production and endothelial NO synthase (NOS) expression and lowered inducible NOS, p22(phox) expression and O(2)(.-)production after 14 and 20 weeks of diet. Protein lipoxidative damage (including 4-hydroxynonenal) was decreased after a long-term FO-diet. This supports the hypothesis that a FO-rich diet could counteract atherogenic oxidative stress, showing beneficial effects of long-chain (n-3) PUFA.
Department of Physiology, INSA, RETICS, University of Barcelona, Spain.
Kelly
K
Casós
María C
MC
Zaragozá
Neven
N
Zarkovic
Kamelija
K
Zarkovic
Luka
L
Andrisic
Manuel
M
Portero-Otín
Daniel
D
Cacabelos
María T
MT
Mitjavila
D000447:Aldehydes / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000517:prevention & control; D003314:Corn Oil / Q000008:administration & dosage / Q000494:pharmacology; D003573:Cytochrome b Group / Q000096:biosynthesis / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects*; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D006938:Hyperlipoproteinemia Type II / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D019255:NADPH Oxidases / Q000096:biosynthesis / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects*
D000447:Aldehydes; D001057:Apolipoproteins E; D003573:Cytochrome b Group; D005395:Fish Oils; C033266:Menhaden oil; D009569:Nitric Oxide; D003314:Corn Oil; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496317:Nos2 protein, mouse; C496315:Nos3 protein, mouse; D019255:NADPH Oxidases; C515614:Cyba protein, mouse; C027576:4-hydroxy-2-nonenal