[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification (LAMP)].
OBJECTIVE
To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).


METHODS
Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.


RESULTS
The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.


CONCLUSIONS
RT-LAMP is more simple and convenient than RT-PCR.
Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, Jilin, China.
Shuang
S
Wang
Jian-hui
JH
Zhou
Xiang
X
Hou
D016679:Genome, Viral / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012412:Rubella virus / Q000235:genetics* / Q000302:isolation & purification*