Serotonin receptor 3A expression in normal and neoplastic B cells.
Chromosome 11q21-qter is a very gene-rich region and is often targeted by genomic aberrations in a variety of human cancers, including B cell lymphoid neoplasms. In order to identify genes which could be deregulated in diffuse large B cell lymphomas (DLBCL), we analyzed the expression levels of genes mapped on chromosome 11q21-qter across a series of publicly available DLBCL expression data sets. We identified HTR3A, the gene coding for 5-hydroxytryptamine receptor 3A, as the most frequently differentially expressed. Since HTR3A expression had not been previously characterized in normal and neoplastic B cells, we evaluated its expression by real-time PCR and by immunohistochemistry. The gene is expressed by B cells and it seems to be regulated during the mature B cell differentiation, being overexpressed during the germinal center phase. Lymphomas derived from germinal center B cells maintain the expression, with the level of expression varying among different patients.
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland.
Andrea
A
Rinaldi
Anna Maria
AM
Chiaravalli
Michael
M
Mian
Emanuele
E
Zucca
Maria Grazia
MG
Tibiletti
Carlo
C
Capella
Francesco
F
Bertoni
D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D001706:Biopsy; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002880:Chromosomes, Human, Pair 11; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008224:Lymphoma, Follicular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C509409:HTR3A protein, human; D012333:RNA, Messenger; D044406:Receptors, Serotonin, 5-HT3