[The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content].
The rhizobacteria Azospirillum brasilense Sp245 produce antigenically different lipopolysaccharides LPSI and LPSII, both containing identical pentasaccharides built from D-rhamnose residues as the repeated chains of O-specific oligosaccharides (OPS). In this study, we report the structure of the OPS from A. brasilense LPSI(-)LPSII(-)-mutant Sp245.5, which spontaneously lost the p85 and p120 plasmids upon the formation of a new 300-MDa megaplasmid after the long-term storage of the bacteria in a rich medium. The repeating unit of the A. brasilense mutant Sp245.5 appeared to be a disaccharide consisting of residues of N-acetyl-D-galactosamine and N-acetyl-D-mannosaminuronic acid: [Formula: see text].
Institute of Biochemistry and Physiology of Plants, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049 Russia. fedonenko@ibppm.sgu.ru
Iu P
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Fedonenko
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EI
Katsy
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LP
Petrova
A S
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Boĭko
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Zdorovenko
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Kachala
A S
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Shashkov
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Knirel'
D015806:Azospirillum brasilense / Q000737:chemistry / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D019081:O Antigens / Q000737:chemistry*; D010957:Plasmids
D019081:O Antigens