Synthesis and characterization of dexamethasone-conjugated linear polyethylenimine as a gene carrier.
Linear polyethylenimine (25 kDa, LPEI25k) has been shown to be an effective non-viral gene carrier with higher transfection and lower toxicity than branched polyethylenimine (BPEI) of comparable molecular weight. In this study, dexamethasone was conjugated to LPEI25k to improve the efficiency of gene delivery. Dexamethasone is a synthetic glucocorticoid receptor ligand. Dexamethasone-conjugated LPEI25k (LPEI-Dexa) was evaluated as a gene carrier in various cells. Gel retardation assays showed that LPEI-Dexa completely retarded plasmid DNA (pDNA) at a 0.75:1 weight ratio (LPEI/pDNA). LPEI-Dexa had the highest transfection efficiency at a 2:1 weight ratio (LPEI-Dexa/DNA). At this ratio, the size of the LPEI-Dexa/pDNA complex was approximately 125 nm and the zeta potential was 35 mV. LPEI-Dexa had higher transfection efficiency than LPEI and Lipofectamine 2000. In addition, the cytotoxicity of LPEI-Dexa was much lower than that of BPEI (25 kDa, BPEI25k). In conclusion, LPEI-Dexa has a high transfection efficiency and low toxicity and can therefore be used for non-viral gene delivery.
Kim
Hyunjung
H
Department of Bioengineering, College of Engineering, Hanyang University, Seoul 133-791, Korea.
Bae
Yun Mi
YM
Kim
Hyun Ah
HA
Hyun
Hyesun
H
Yu
Gwang Sig
GS
Choi
Joon Sig
JS
Lee
Minhyung
M
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D003907:Dexamethasone / Q000138:chemical synthesis*; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005938:Glucocorticoids / Q000138:chemical synthesis*; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D011094:Polyethyleneimine / Q000138:chemical synthesis*; D051381:Rats; D014162:Transfection
D005938:Glucocorticoids; D003907:Dexamethasone; D011094:Polyethyleneimine