Identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs.
Bacterial agents are considered important pathogens causing external otitis in dogs. It is essential to carry out bacterial culture and antimicrobial susceptibility test in the case of otitis externa, particularly for chronic or recurring cases. Sterile swab samples were obtained from terminal part of vertical ear canals of 74 dogs with otitis externa for cytology, bacterial culture and antimicrobial susceptibility test. Cytologic smears were stained using Gram and Giemsa staining methods. Aerobic bacterial culture performed on blood agar and MacConkey agar. Among total number of 92 isolated bacteria, 68 were Staphylococcus intermedius. Other isolated bacteria included: Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Pasteurella canis, and six other species of coagulase-negative Staphylococcus. Antimicrobial susceptibility test were performed for all isolated bacteria using 14 antibiotics. Based on the results of this study, all isolated Staphylococcus spp. were sensitive to amikacin, enrofloxacin, and rifampin, and had low resistance to gentamicin, cephalothin and ceftriaxone. More than half of gram-positive isolates were resistant to penicillin and ampicillin. Generally, all isolated gram-negative bacteria, were sensitive to amikacin and enrofloxacin, and had low resistance to ceftriaxone and gentamicin. They were highly resistant to penicillin, eythromycin, and cephalothin. Regarding the results of this study, in cases of uncomplicated otitis externa, it is possible to select antimicrobial drugs merely based on cytology, but it is recommended to perform bacterial culture and antimicrobial susceptibility test. However, in complicated or refractory cases, antimicrobials should be selected based on bacterial culture and antimicrobial susceptibility test.
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Gharib St, Azadi St, P.O. Box 14155-6453, Tehran, Iran.
Hamed
H
Zamankhan Malayeri
Shahram
S
Jamshidi
Taghi
T
Zahraei Salehi
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D010032:Otitis Externa / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D010325:Pasteurella / Q000187:drug effects; D010326:Pasteurella Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D011512:Proteus Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D011513:Proteus mirabilis / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000187:drug effects
D000890:Anti-Infective Agents