Evidence of a tumour suppressor function for DLEC1 in human breast cancer.
DLEC1 (deleted in lung and oesophageal cancer), located on 3p22.3, is a candidate tumour suppressor gene in lung, esophageal, and renal cancer. The aim of this study was determine whether the mRNA expression levels of DLEC1 were consistent with a tumour suppressive function.


METHODS
A total of 153 samples were analysed. The levels of transcription of DLEC1 were determined using quantitative PCR and normalised against (CK19). Transcript levels within breast cancer specimens were compared to normal background tissues.


RESULTS
Levels of transcription were lower [corrected] in tumour samples compared to adjacent non cancerous tissue (ANCT) samples but this was not statistically significant (median 0.167 vs. 0.03; p=0.138). DLEC1 expression levels were significantly lower in samples from patients who developed metastasis, local recurrence, or died of breast cancer when compared to those who were disease free for >10 years (p=0.041).


CONCLUSIONS
These findings are consistent with a possible tumour suppressor function of DLEC1 in breast cancer.
St. George's University of London, Tooting, London, UK.
W
W
Al Sarakbi
S
S
Reefy
W G
WG
Jiang
T
T
Roberts
R F
RF
Newbold
K
K
Mokbel
D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*
C119064:DLEC1 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins