A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae.
Plant vacuolar H(+)-ATPase (V-ATPase) plays an important role in response to different adverse environmental conditions. In the present study, we cloned and characterized a V-ATPase c subunit gene (ThVHAc1) from Tamarix hispida. The deduced ThVHAc1 amino acid sequence lacks a signal peptide and ThVHAc1 is a highly hydrophobic protein with four transmembrane regions. A transient expression assay showed that the ThVHAc1-GFP fusion protein is expressed on onion epidermal endomembrane cells. Real-time RT-PCR demonstrated that ThVHAc1 gene expression was induced by NaCl, NaHCO(3), PEG and CdCl(2) stress in T. hispida roots, stems and leaves. Exogenous application of abscisic acid (ABA) also stimulated ThVHAc1 transcript levels in the absence of stress, suggesting that ThVHAc1 is involved in ABA-dependent stress signaling pathway. Furthermore, the transgenic yeast expressing ThVHAc1 increased salt, drought, ultraviolet (UV), oxidative, heavy metal, cold and high temperature tolerance. Our results suggested that the ThVHAc1 gene from T. hispida serves a stress tolerance role in the species.
Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Ministry of Education, 26 Hexing Road, 150040, Harbin, People's Republic of China.
Caiqiu
C
Gao
Yucheng
Y
Wang
Bo
B
Jiang
Guifeng
G
Liu
Lili
L
Yu
Zhigang
Z
Wei
Chuanping
C
Yang
D000040:Abscisic Acid / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019697:Onions / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D032363:Tamaricaceae / Q000378:metabolism*; D013997:Time Factors; D019076:Transgenes; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics*
D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D000040:Abscisic Acid; D025262:Vacuolar Proton-Translocating ATPases