Association of Asn221Ser mutation in tissue factor pathway inhibitor-beta with plasma total tissue factor pathway inhibitor level.
Tissue factor pathway inhibitor (TFPI) is an anticoagulant protease inhibitor that inhibits the tissue factor-initiated blood coagulation cascade reactions. Based on these anticoagulant functions of TFPI, we hypothesized that genetic variations in TFPI may alter the TFPI expression or impair the anticoagulant function and could predispose persons to deep vein thrombosis (DVT). This study was undertaken to examine whether the genetic polymorphisms in TFPI are associated with the plasma TFPI levels and risk for DVT. We sequenced the entire coding regions of TFPI in 175 Japanese DVT patients and identified 12 genetic variants, including one missense mutation, Asn221Ser. The missense mutation occurred at the site presumably attached to the glycosylphosphatidylinositol anchor in the TFPI-beta form. The allele frequency of the mutant Ser-coding allele of the Asn221Ser mutation was 8% in the Japanese general population consisting of 1684 individuals. The Asn221Ser mutation was significantly associated with the total TFPI levels (Asn/Asn, n = 108, total TFPI = 56.57 +/- 0.88 ng/ml (mean +/- SD) vs. Asn/Ser + Ser/Ser, n = 16, total TFPI = 63.44 +/- 2.28 ng/ml, P = 0.0058). The genotype was not associated with the free TFPI level. This Asn221Ser mutation was not associated with DVT. Thus, the Asn221Ser mutation occurring in the TFPI-beta form was associated with the total TFPI level, but not a risk for DVT. The absence of the putative glycosylphosphatidylinositol anchor in TFPI-beta under pathological conditions remains to be studied.
National Cardiovascular Center Research Institute, Osaka, Japan.
Junko
J
Ishikawa
Hiromi
H
Okada
Hisao
H
Kato
Satoshi
S
Takeshita
Shigenori
S
Honda
Tomio
T
Kawasaki
Etsuji
E
Suehisa
Hajime
H
Tsuji
Seiji
S
Madoiwa
Yoichi
Y
Sakata
Tetsuhito
T
Kojima
Mitsuru
M
Murata
Yasuo
Y
Ikeda
Yoshihiro
Y
Kokubo
Tomonori
T
Okamura
Hitonobu
H
Tomoike
Toshiyuki
T
Miyata
D000483:Alleles; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007564:Japan; D008074:Lipoproteins / Q000097:blood* / Q000235:genetics*; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D020246:Venous Thrombosis / Q000097:blood / Q000235:genetics
D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor