Label-free imaging of human cells: algorithms for image reconstruction of Raman hyperspectral datasets.
Raman microspectroscopy-based, label-free imaging methods for human cells at sub-micrometre spatial resolution are presented. Since no dyes or labels are used in this imaging modality, the pixel-to-pixel spectral variations are small and multivariate methods of analysis need to be employed to convert the hyperspectral datasets to spectral images. Thus, the main emphasis of this paper is the introduction and comparison of a number of multivariate image reconstruction methods. The resulting Raman spectral imaging methodology directly utilizes the spectral contrast provided by small (bio)chemical compositional changes over the spatial dimension of the sample to construct images that can rival fluorescence images in terms of spatial information, yet without the use of any external dye or label.
Department of Chemistry and Chemical Biology, Northeastern University, 316 Hurtig Hall, Boston, MA 02115, USA. milos.miljkovic@yahoo.com
Milos
M
Miljković
Tatyana
T
Chernenko
Melissa J
MJ
Romeo
Benjamin
B
Bird
Christian
C
Matthäus
Max
M
Diem
D000465:Algorithms; D016208:Databases, Factual; D006801:Humans; D015999:Multivariate Analysis; D013059:Spectrum Analysis, Raman