Complex refractive index measurement of biological tissues by attenuated total reflection ellipsometry.
To apply reflection ellipsometry to determine the real and imaginary parts of the refractive index of biological tissues simultaneously, we combine reflection ellipsometry with total internal reflection to warrant minimal influences by the strong scattering and absorption of biological tissues. A K9 glass prism with refractive index 1.51468 at wavelength 632.8 nm and a Glan prism polarizer with an angular sampling interval of 0.1 degrees were used in our experimental setup. Using the setup, the complex refractive indices of some typical mammalian tissues were measured under the wavelength of 632.8 nm. The results show that the indices of porcine muscle, liver, pancreas, and dermis tissues were 1.3713+0.062i, 1.3791+0.0087i, 1.3517+0.0113i, and 1.3818+0.0049i, respectively.
School of Science, Nanjing University of Science & Technology, Nanjing 210094, China. laijiancheng@mail.njust.edu.com
Jian-Cheng
JC
Lai
Ying-Ying
YY
Zhang
Zhen-Hua
ZH
Li
Hai-Jiao
HJ
Jiang
An-Zhi
AZ
He
D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D010783:Photometry / Q000379:methods*; D012031:Refractometry / Q000379:methods*