The biocompatibility of amino functionalized CdSe/ZnS quantum-dot-Doped SiO2 nanoparticles with primary neural cells and their gene carrying performance.
Nanoparticles have an enormous potential for the development of applications in biomedicine such as gene or drug delivery. We developed and characterized NH(2) functionalized CdSe/ZnS quantum dot (QD)-doped SiO(2) nanoparticles (NPs) with both imaging and gene carrier capabilities. We show that QD-doped SiO(2) NPs are internalized by primary cortical neural cells without inducing cell death in vitro and in vivo. Moreover, the ability to bind, transport and release DNA into the cell allows GFP-plasmid transfection of NIH-3T3 and human neuroblastoma SH-SY5Y cell lines. QD-doped SiO(2) NPs properties make them a valuable tool for future nanomedicine application.
Bardi
Giuseppe
G
CNR Neuroscience Institute, Pisa, Italy. giuseppe.bardi@in.cnr.it
Malvindi
Maria Ada
MA
Gherardini
Lisa
L
Costa
Mario
M
Pompa
Pier Paolo
PP
Cingolani
Roberto
R
Pizzorusso
Tommaso
T
D000818:Animals; D017209:Apoptosis; D019187:Cadmium Compounds / Q000737:chemistry; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000166:cytology*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry; D014162:Transfection; D017967:Zinc Compounds / Q000737:chemistry
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D012822:Silicon Dioxide; D004247:DNA; C058667:cadmium selenide; C031238:zinc sulfide