Improvement of polyunsaturated fatty acids synthesis by the coexpression of CYB5 with desaturase genes in Saccharomyces cerevisiae.
Since Saccharomyces cerevisiae contains Delta9 fatty acid desaturase (OLE1) as a sole fatty acid desaturase, it produces saturated and monounsaturated fatty acids of 16- and 18-carbon compounds. We showed earlier that Kluyveromyces lactis Delta12 (KlFAD2) and omega3 (KlFAD3) fatty acid desaturase genes enabled S. cerevisiae to make also polyunsaturated fatty acids (PUFAs), linoleic (18:2n-6), and alpha-linolenic (18:3n-3) acids. Unlike Delta9 fatty acid desaturase Ole1p, the two added fatty acid desaturases (KlFAD2and KlFAD3) do not contain a cytochrome b5 domain, and we now report on effects of the overexpression of K. lactis and S. cerevisiae cytochrome b5 (CYB5) genes as well as temperature effects on PUFA synthesis. Without extra cytochrome b5, while PUFA synthesis is significant at low temperature (20 degrees C), it was marginal at 30 degrees C. Overexpression of cytochrome b5 at 20 degrees C did not affect the fatty acid synthesis so much, but it significantly enhanced the synthesis of PUFA at 30 degrees C.
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central, Ibaraki, Japan.
Hisashi
H
Yazawa
Hitoshi
H
Iwahashi
Yasushi
Y
Kamisaka
Kazuyoshi
K
Kimura
Hiroshi
H
Uemura
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015786:Cytochromes b5 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D015870:Gene Expression; D007716:Kluyveromyces / Q000737:chemistry / Q000201:enzymology*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D029701:Saccharomyces cerevisiae Proteins; D015786:Cytochromes b5; D044943:Fatty Acid Desaturases