Vectors and parameters that enhance the efficacy of RNAi-mediated gene disruption in transgenic Drosophila.
Whole-genome transgenic RNAi libraries permit systematic genetic screens in individual tissues of Drosophila. However, there is a high incidence of nonspecific phenotypes because of off-target effects. To minimize such effects, it is essential to obtain a deeper understanding of the specificity of action of RNAi. Here, in vivo assays are used to determine the minimum, contiguous nucleotide pairing required between an siRNA and a target mRNA to generate a phenotype. We observe that as few as 16 nucleotides of contiguous homology are sufficient to attenuate gene activity. This finding provides an explanation for the high incidence of off-target effects observed in RNAi-based genetic screens. Toward improving the efficacy of RNAi-induced phenotypes in vivo, we describe siRNA expression vectors that allow coexpression of one or more siRNAs with a fluorescent reporter gene in cultured cells or transgenic flies. This expression system makes use of the small intron from the ftz segmentation gene to provide efficient processing of synthetic siRNAs from a reporter transcript. These studies provide a foundation for the specific and effective use of gene silencing in transgenic Drosophila.
Haley
Benjamin
B
Center for Integrative Genomics, Division of Genetics, Genomics, and Development, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Foys
Bryon
B
Levine
Michael
M
D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques; D004330:Drosophila / Q000235:genetics*; D005456:Fluorescent Dyes / Q000494:pharmacology; D020868:Gene Silencing; D017930:Genes, Reporter; D005821:Genetic Techniques; D005822:Genetic Vectors; D007438:Introns; D008957:Models, Genetic; D010641:Phenotype; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
D005456:Fluorescent Dyes; D012333:RNA, Messenger; D034741:RNA, Small Interfering