Increased mRNA expression of interferon-induced Mx1 and immunomodulation following oral administration of IFN-alpha2b-transformed B. longum to mice.
We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene transcription in mice. We observed enhanced serum and fecal IFN-alpha2b concentrations in mice orally administered recombinant B. longum, suggesting a possible Th1 pattern of induction in the spleen and Peyer's patches. Transcription of the typically IFN-induced antiviral Mx1 gene in the hepatic and intestinal tissues of these mice was also markedly enhanced. In conclusion, oral administration of the recombinant B. longum expressing IFN-alpha2b might play its roles in the immunomodulation of the mice, and the potential clinical value of this bacterium in the treatment of viral infections needs to be further studied.
Yu
Zhijian
Z
Department of Infectious Diseases, The Affiliated Shenzhen Nanshan Hospital of Guangdong Medical College, No 89 Taoyuan Road, Nanshan District, Shenzhen 518052, China.
Zeng
Zhongming
Z
Huang
Zhen
Z
Lian
Jie
J
Yang
Jin
J
Deng
Qiwen
Q
Zeng
Weiseng
W
D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium; D016207:Cytokines / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D056747:Immunomodulation; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage* / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D064547:Myxovirus Resistance Proteins; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism; D019936:Probiotics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic
D016207:Cytokines; D000077190:Interferon alpha-2; D016898:Interferon-alpha; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins