db-Cyclic adenosine monophosphate promotes axon regeneration and motor function recovery in cerebral ischemia-reperfusion rats.
BACKGROUND
There is no effective axon regeneration in adult mammalians.


OBJECTIVE
To investigate the effects of dual-acid cyclic adenosine monophosphate (db-cAMP) on the axon regeneration, motor function recovery and RhoA signal pathway in cerebral ischemia-reperfusion rats, and to explore the possible clinical application and mechanism.


METHODS
Middle cerebral artery ischemia-reperfusion model was established by nylon monofilament occlusion method in 105 Sprague-Dawley (SD) rats. Semi-quantitative Western blot analysis was used to assess protein expression level of growth-associated protein-43 (GAP-43) and RhoA. Montoya staircase test score was used to test the motor function of affected forelimb.


RESULTS
Compared to the ischemia group, the staircase test score in the db-cAMP group was increased significantly at 30-day (P < 0.05), and GAP-43 protein expression in the db-cAMP group was enhanced significantly at 7-day and 14-day (P < 0.05), and RhoA protein expression in the db-cAMP group was decreased significantly between 24 h to 14-day (P < 0.01).


CONCLUSIONS
These results show that db-cAMP can promote axon regeneration and the recovery of motor function by inhibiting RhoA signal pathway.
Department of Neurology, The Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China.
Lingchuan
L
Niu
Jia
J
Zhou
Ying
Y
Huang
Yan
Y
Chen
Changqing
C
Li
D000818:Animals; D001369:Axons / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology* / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005552:Forelimb / Q000503:physiopathology; D019922:GAP-43 Protein / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D011597:Psychomotor Performance / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D015424:Reperfusion; D013997:Time Factors; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D019922:GAP-43 Protein; D000242:Cyclic AMP; D020741:rho GTP-Binding Proteins