Amperometric glucose biosensor with remarkable acid stability based on glucose oxidase entrapped in colloidal gold-modified carbon ionic liquid electrode.
A colloidal gold-modified carbon ionic liquid electrode was constructed by mixing colloidal gold-modified graphite powder with a solid room temperature ionic liquid n-octyl-pyridinium hexafluorophosphate (OPPF(6)). Glucose oxidase (GOD) was entrapped in this composite matrix and maintained its bioactivity well and displayed excellent stability. The effect conditions of pH, applied potential and GOD loading were examined. Especially, the glucose oxidase entrapped in this carbon ionic liquid electrode fully retained its activity upon stressing in strongly acidic conditions (pH 2.0) for over one hour. The proposed biosensor responds to glucose linearly over concentration range of 5.0x10(-6) to 1.2x10(-3) and 2.6x10(-3) to 1.3x10(-2) M, and the detection limit is 3.5x10(-6) M. The response time of the biosensor is fast (within 10s), and the life time is over two months. The effects of electroactive interferents, such as ascorbic acid, uric acid, can be significantly reduced by a Nafion film casting on the surface of resulting biosensor.
Liu
Xiaoying
X
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha, PR China.
Zeng
Xiandong
X
Mai
Nannan
N
Liu
Yong
Y
Kong
Bo
B
Li
Yonghong
Y
Wei
Wanzhi
W
Luo
Shenglian
S
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D017979:Gold Colloid; D006108:Graphite; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids; D057230:Limit of Detection
D004800:Enzymes, Immobilized; D017979:Gold Colloid; D052578:Ionic Liquids; D002244:Carbon; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose