Fluorescent labeling of (oligo)nucleotides by a new fluoride cleavable linker capable of versatile attachment modes.
The development of a fluoride cleavable linker 1 for reversibly labeling (oligo)nucleotides is described here. The linker allows different ways of chemical attachment of a reporter molecule, for example, click chemistry or amide formation. The versatile attachment modes of labels are demonstrated by derivatizations with pyrene and fluorescein. Besides the synthesis of the new linker, we also show the derivatization of iodobenzene as a model compound and a nucleoside to demonstrate the applicability. Further, cleavability studies in solution and on a solid-supported oligonucleotide are shown. The linker can be applied in the synthesis of reversible terminators, useful for new DNA sequencing technologies like cyclic reversibly terminating (CRT) sequencing.
Institut fur Organische Chemie und Chemische Biologie, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany.
Diana C
DC
Knapp
Jennifer
J
D'Onofrio
Joachim W
JW
Engels
D002851:Chromatography, High Pressure Liquid; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005459:Fluorides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011721:Pyrenes; C030984:pyrene; D005459:Fluorides; D019793:Fluorescein