Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
OBJECTIVE
There is a compelling clinical imperative to identify discerning molecular biomarkers of hepatic disease in order to inform the diagnosis, prognosis and treatment.


METHODS
We have investigated the proteome of urinary vesicles present in urine samples obtained from experimental models for the study of liver injury, as an approach for identifying potential biomarkers for hepatic disease.


RESULTS
The biochemical and proteomic characterization of highly purified exosome-like urinary vesicles has identified 28 proteins previously unreported in these vesicles, and many that have been previously associated with diseases, such as the prion-related protein. Furthermore, in urine samples from D-galactosamine-treated rats, a well-characterized experimental model for acute liver injury, we have detected a severe reduction in some proteins that normally are clearly detected in urinary vesicles. Finally, differential protein content on urinary vesicles from a mouse model for chronic liver injury has been also identified.


CONCLUSIONS
Our results argue positively that urinary vesicles could be a source for identifying non-invasive biomarkers of liver injury. We proposed some proteins such as Cd26, Cd81, Slc3A1 and Cd10 that have been found to be differentially expressed in urinary vesicles from some of the analyzed models as potential biomarkers for liver injury.
Metabolomics Unit, CICbioGUNE, CIBERehd, Bizkaia Technology Park, Derio, Bizkaia, Spain.
Javier
J
Conde-Vancells
Eva
E
Rodriguez-Suarez
Esperanza
E
Gonzalez
Agustin
A
Berisa
David
D
Gil
Nieves
N
Embade
Mikel
M
Valle
Zigmund
Z
Luka
Felix
F
Elortza
Conrad
C
Wagner
Shelly C
SC
Lu
Jose M
JM
Mato
M
M
Falcon-Perez
D000818:Animals; D015415:Biomarkers / Q000652:urine*; D015153:Blotting, Western; D004195:Disease Models, Animal; D055354:Exosomes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000293:injuries / Q000473:pathology; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers