How well quantified is the limit of quantification?
BACKGROUND
Raw data on the relationship between known and measured values of an analyte are collected and analyzed to determine the limit of quantification (LOQ) of an assay. In most LOQ problems, the researcher is given an observed value for the marker of interest if this value is greater than the LOQ, and a missing value (<LOQ) otherwise. From a statistical perspective, the implicit assumption is that there is no measurement error for values greater than the LOQ, and unacceptable measurement error for values less than the LOQ. A more plausible assumption is that there is measurement error throughout the measure's support.


METHODS
We describe a Bayesian measurement error model that yields prediction intervals for the true assay value throughout the range of analyte values, and allows for heteroscedasticity of the measurement errors.


RESULTS
We illustrate our model on calibration data for fat-soluble vitamins, focusing particularly on beta-cryptoxanthin. Prediction intervals for values above the LOQ are wide, and the width increases with the measured value. Prediction intervals below the LOQ provide more information than the statement that the value is less than the LOQ.


CONCLUSIONS
The current approach to transmitting data from calibration assays is flawed, since it provides a distorted picture of the actual measurement error. Implications for subsequent analyses of assay measurements are discussed.
Department of Biostatistics, University of Michigan, Ann Arbor, MI 48109, USA.
Ying
Y
Guo
Ofer
O
Harel
Roderick J
RJ
Little
D016588:Anticarcinogenic Agents / Q000097:blood; D001499:Bayes Theorem; D065366:Cryptoxanthins; D016021:Epidemiologic Studies; D057230:Limit of Detection; D015233:Models, Statistical; D014815:Vitamins / Q000097:blood; D024341:Xanthophylls / Q000097:blood
D016588:Anticarcinogenic Agents; D065366:Cryptoxanthins; D014815:Vitamins; D024341:Xanthophylls