Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division.
The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) regulates cellular motility and the synthesis of organelles and molecules that promote adhesion to a variety of biological and nonbiological surfaces. These properties likely require tight spatial and temporal regulation of c-di-GMP concentration. We have developed genetically encoded fluorescence resonance energy transfer (FRET)-based biosensors to monitor c-di-GMP concentrations within single bacterial cells by microscopy. Fluctuations of c-di-GMP were visualized in diverse Gram-negative bacterial species and observed to be cell cycle dependent. Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa. Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function.
Department of Immunology, University of Washington, Seattle, WA 98195, USA.
Matthias
M
Christen
Hemantha D
HD
Kulasekara
Beat
B
Christen
Bridget R
BR
Kulasekara
Lucas R
LR
Hoffman
Samuel I
SI
Miller
D015374:Biosensing Techniques; D016935:Caulobacter crescentus / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D029968:Escherichia coli Proteins; D031541:Fluorescence Resonance Energy Transfer; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000378:metabolism; D008853:Microscopy; D009068:Movement; D009154:Mutation; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000166:cytology / Q000378:metabolism; D015290:Second Messenger Systems
D029968:Escherichia coli Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP