Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis.
Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic T(H)1 cytokines (interleukin-12, interleukin-18, interferon-gamma, tumor necrosis factor-alpha) and regulatory transforming growth factor-beta(1)), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the T(H)1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-beta(1). In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic T(H)1 response.
Cukrowska
B
B
Department of Pathology, The Children's Memorial Health Institute, Warsaw, Poland. b.cukrowska@czd.pl
Rosiak
I
I
Klewicka
E
E
Motyl
I
I
Schwarzer
M
M
Libudzisz
Z
Z
Kozakova
H
H
D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007223:Infant; D007778:Lactobacillus / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D019936:Probiotics / Q000494:pharmacology
D016207:Cytokines; D007155:Immunologic Factors