NMDA receptor in intestinal enteroendocrine cell, STC-1.
We earlier showed that intestinal STC-1 cells can respond to five basic taste stimuli. Here, we investigated which receptors or channels are involved in the sensing of umami in STC-1 cells. T1R family and metabotropic glutamate receptors were not involved. In contrast, we found that N-Methyl-D-aspartate (NMDA) receptors are expressed in STC-1 cells by RT-PCR analysis. Using a calcium-imaging technique, we also observed that a specific agonist NMDA induces the increase in the intracellular Ca concentration in STC-1 cells. We also found that pretreatment with NMDA enhances sweet-induced response in STC-1 cells, suggesting important roles of L-Glu-signaling in the chemosensory system of the gastrointestinal tracts.
Fukunaga
Yoichiro
Y
Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama-shi, Shiga, Japan.
Kimura
Mayuko
M
Saitoh
Osamu
O
D000818:Animals; D002460:Cell Line; D002628:Chemoreceptor Cells / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism*; D051379:Mice; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016194:Receptors, N-Methyl-D-Aspartate