Effects of hesperetin on the production of inflammatory mediators in IL-1beta treated human synovial cells.
This study was conducted to evaluate the efficacy of hesperetin in regulating interleukin-1beta (IL-1beta)-induced production of the matrix metalloproteinase (MMP)-3 and IL-6 in human synovial cell line, SW982. Treatment with hesperetin at 1 or 10 microM significantly (P<0.05) inhibited IL-1beta-induced MMP-3 and IL-6 production when measured by enzyme-linked immunosorbent assay (ELISA). The effects of hesperetin on the activation of mitogen-activated protein kinases (MAPKs) were also examined in SW982 cells by ELISA assay. IL-1beta-induced JNK activation was inhibited by hesperetin. These results suggest that hesperetin reduces the production of MMP and IL-6 in SW982 synovial cells by inhibiting JNK.
Department of Food & Nutrition, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea. cheunmi@hanmail.net
Eun Mi
EM
Choi
Young Soon
YS
Lee
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000178:diet therapy / Q000188:drug therapy* / Q000276:immunology; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005638:Fruit; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013583:Synovial Membrane / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D007375:Interleukin-1; D015850:Interleukin-6; D006569:Hesperidin; D048049:Extracellular Signal-Regulated MAP Kinases; D048670:MAP Kinase Kinase 4; D019278:Matrix Metalloproteinase 3; C013015:hesperetin