Development of molecular docking-based binding energy to predict the joint effect of BPA and its analogs.
A general proposal for predicting the joint effect of endocrine disrupting chemicals by examining binding energy models was developed in this study. 2,2-bis(4-hydroxyphenyl)propane (BPA) and 11 of its analogs were chosen, and the estrogenic activity of each compound was measured by determining its EC50 value using a recombinant gene yeast assay. Binding energies (BEs) were calculated using Surflex-Docking software. The analysis of the relationship between EC50 values and BEs showed that there is a linear correlation between the BEs and EC50 values. Furthermore, the analysis of the given binary and quaternary mixtures of BPA and three of its analogs showed that the joint effects of the mixtures were affected by the proportions of the chemicals in each mixture and their relative binding energy. The correlation between the joint effects of mixtures and the binding energy of the individual compounds has been described using one formula, which can be used to predict the joint effects of other mixtures.
Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, China.
Hong-Chang
HC
Zhang
Xia-Lin
XL
Hu
Da-Qiang
DQ
Yin
Zhi-Fen
ZF
Lin
D001559:Benzhydryl Compounds; D004338:Drug Combinations; D004968:Estrogens, Non-Steroidal / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D010636:Phenols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011237:Predictive Value of Tests; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000187:drug effects; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D015003:Yeasts / Q000187:drug effects / Q000235:genetics; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004338:Drug Combinations; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D011960:Receptors, Estrogen; C006780:bisphenol A; D000098826:Bisphenol A Compounds