Leaf extract of Wasabia japonica relieved oxidative stress induced by Helicobacter pylori infection and stress loading in Mongolian gerbils.
Infection with Helicobacter pylori (H. pylori) can induce gastric disorders, and though its presence cannot explain disease pathogenesis and does not have associations with other factors, it is well known that H. pylori infection causes stomach inflammation following oxidative stress. We examined the suppressive effects of a leaf extract of Wasabia japonica on H. pylori infection and on stress loading in Mongolian gerbils. Following oral administration of wasabi extract of 50 and 200 mg/kg B.W./d for 10 d, the animals were exposed to restraint stress for 90 and 270 min. As for the results, the level of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in the stomach and oxidative DNA damage in peripheral erythrocytes at 270 min significantly increased. That elevation was significantly suppressed by the addition of the leaf extract. We concluded that the simultaneous loading of H. pylori infection and physical stress loading might induce oxidative DNA damage additively, while a leaf extract attenuated this DNA damage in the stomach as well as the peripheral erythrocytes.
Sekiguchi
Hirotaka
H
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka, Shizuoka, Japan.
Takabayashi
Fumiyo
F
Deguchi
Yuya
Y
Masuda
Hideki
H
Toyoizumi
Tomoyasu
T
Masuda
Shuichi
S
Kinae
Naohide
N
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016480:Helicobacter pylori / Q000502:physiology*; D008297:Male; D008986:Mongolia; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D012149:Restraint, Physical / Q000009:adverse effects; D013270:Stomach / Q000187:drug effects / Q000382:microbiology; D013315:Stress, Psychological / Q000188:drug therapy / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D031228:Wasabia / Q000737:chemistry*
D010936:Plant Extracts; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine