Preparation of DNA and RNA fragments containing guanine N(2)-thioalkyl tethers.
This unit describes procedures for preparation of deoxyguanosine and guanosine derivatives in which the guanine N(2) contains a thiopropyl tether, protected as a tert-butyl disulfide. After incorporation into a DNA or RNA fragment, this tether allows site-specific cross-linking to a thiol of a protein or another nucleic acid.
Rutgers, The State University of New Jersey, Piscataway, New Jersey, USA.
Xiaorong
X
Hou
Gang
G
Wang
Barbara L
BL
Gaffney
Roger A
RA
Jones
D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D003998:Dicarboxylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D003998:Dicarboxylic Acids; D004220:Disulfides; D006834:Hydrazines; D009943:Organophosphorus Compounds; D013438:Sulfhydryl Compounds; C434331:phosphoramidite; C029424:hydrazine; D006147:Guanine; D012313:RNA; D004247:DNA