Synthesis and cytotoxic activity of G3 PAMAM-NH(2) dendrimer-modified digoxin and proscillaridin A conjugates in breast cancer cells.
The objective of this study was to determine the cytotoxicity, antiproliferative activity, and apoptosis induction activity of two modified glycosides - digoxin and proscillaridin A - conjugated to a generation 3 polyamidoamine dendrimer (G3 PAMAM-NH(2)) in human breast cancer cells. The results suggest that conjugation with the G3 PAMAM-NH(2) dendrimer enhances the cytotoxicity of modified digoxin and proscillaridin Aboth in MCF-7 and in MDA-MB-231 breast cancer cells. Additionally, the conjugate-induced apoptosis was significantly greater than apoptosis evoked by free modified digoxin and proscillaridin A.
Department of Pharmaceutical Technology, Medical University of Białystok, Kilińskiego 1, PL 15-089 Białystok, Poland. kwin@umwb.edu.pl
Katarzyna
K
Winnicka
Krzysztof
K
Bielawski
Anna
A
Bielawska
D000970:Antineoplastic Agents / Q000138:chemical synthesis*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers / Q000138:chemical synthesis* / Q000494:pharmacology; D004077:Digoxin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D006801:Humans; D011442:Proscillaridin / Q000031:analogs & derivatives* / Q000494:pharmacology
D000970:Antineoplastic Agents; D050091:Dendrimers; C104700:PAMAM Starburst; D004077:Digoxin; D011442:Proscillaridin