A new lead chemical for strigolactone biosynthesis inhibitors.
Several triazole-containing chemicals have previously been shown to act as efficient inhibitors of cytochrome P450 monooxygenases. To discover a strigolactone biosynthesis inhibitor, we screened a chemical library of triazole derivatives to find chemicals that induce tiller bud outgrowth of rice seedlings. We discovered a triazole-type chemical, TIS13 [2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol], which induced outgrowth of second tiller buds of wild-type seedlings, as observed for non-treated strigolactone-deficient d10 mutant seedlings. TIS13 treatment reduced strigolactone levels in both roots and root exudates in a concentration-dependent manner. Co-application of GR24, a synthetic strigolactone, with TIS13 canceled the TIS13-induced tiller bud outgrowth. Taken together, these results indicate that TIS13 inhibits strigolactone biosynthesis in rice seedlings. We propose that TIS13 is a new lead compound for the development of specific strigolactone biosynthesis inhibitors.
Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657 Japan.
Shinsaku
S
Ito
Nobutaka
N
Kitahata
Mikihisa
M
Umehara
Atsushi
A
Hanada
Atsutaka
A
Kato
Kotomi
K
Ueno
Kiyoshi
K
Mashiguchi
Junko
J
Kyozuka
Koichi
K
Yoneyama
Shinjiro
S
Yamaguchi
Tadao
T
Asami
D018525:Germination; D007783:Lactones / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012275:Oryza / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D036226:Seedlings / Q000254:growth & development; D014230:Triazoles / Q000737:chemistry*
C552858:2,2-dimethyl-7-phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol; C507382:GR24 compound; D007783:Lactones; D010937:Plant Growth Regulators; D014230:Triazoles