Maternal supplementation with a complex milk lipid mixture during pregnancy and lactation alters neonatal brain lipid composition but lacks effect on cognitive function in rats.
Complex milk lipids (CMLs) provide a critical nutritional source for generating both energy and essential nutrients for the growth of the newborn. The present study investigated nutritional supplementation with a CML containing gangliosides and phospholipids in pregnant and lactating rats on learning behavior and postnatal growth in male offspring. Wistar female rats were supplemented during pregnancy and lactation with either control or CML to provide gangliosides at a dose of 0.01% (low) and 0.05% (high) based on total food intake. The CML-supplemented dams showed no differences in comparison to controls regarding growth, food intake, and litter characteristics. There were significant differences in brain composition in male offspring at postnatal day 2 (P2) with higher concentrations of gangliosides (high dose, P < .05) and lower concentrations of phospholipids (low and high dose, P < .05) in the CML-supplemented groups. The distribution of individual ganglioside species was not significantly different between treatment groups. Brain weight at P2 was also significantly higher in the CML groups. Differences in the brain composition and weight were not significant by weaning (P21). As adults (P80), adiposity was reduced in the low CML-supplemented group compared to controls. No significant differences were detected between any of the treatment groups in any of the behavioral tasks (water maze, object recognition, and operant learning). These data suggest that maternal supplementation with a CML during pregnancy and lactation is safe and has a significant early impact on brain weight and ganglioside and phospholipid content in offspring but did not alter long-term behavioral function using standard behavioral techniques.
Gustavsson
Malin
M
Liggins Institute and The National Research Centre for Growth and Development, University of Auckland, Auckland, New Zealand.
Hodgkinson
Steve C
SC
Fong
Bertram
B
Norris
Carmen
C
Guan
Jian
J
Krageloh
Christian U
CU
Breier
Bernhard H
BH
Davison
Michael
M
McJarrow
Paul
P
Vickers
Mark H
MH
D050154:Adiposity; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D019587:Dietary Supplements; D005260:Female; D005732:Gangliosides / Q000378:metabolism / Q000494:pharmacology*; D007774:Lactation; D007858:Learning / Q000187:drug effects; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena; D008892:Milk; D009765:Obesity / Q000517:prevention & control*; D009929:Organ Size; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D005732:Gangliosides; D010743:Phospholipids