Cloning of a novel dehalogenase from environmental DNA.
Cloning of pceA, the gene of tetrachloroethene (PCE)-reductive dehalogenase, was undertaken from environmental DNA. Two genes were amplified using PCR primer deduced from pceA. Functional expression of these genes was unsuccessful in Escherichia coli, but PceA1 synthesized in vitro was enzymatically active. In recombinant E. coli, PceA1 formed a complex with host DnaK and caused filamentous growth.
Department of Bioscience, Fukui Prefectural University, Fukui, Japan. kimoto@fpu.ac.jp
Hisashi
H
Kimoto
Shin-ichiro
S
Suye
Hirokazu
H
Makishima
Jun-ichirou
J
Arai
Sachiko
S
Yamaguchi
Yutaka
Y
Fujii
Toshihito
T
Yoshioka
Akira
A
Taketo
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004777:Environment; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004247:DNA; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase