Aging sensitizes rapidly isolated hippocampal microglia to LPS ex vivo.
The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1β and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.
Frank
Matthew G
MG
Department of Psychology and Neuroscience, University of Colorado, Boulder, CO 80309-0345, USA. matt.frank@colorado.edu
Barrientos
Ruth M
RM
Watkins
Linda R
LR
Maier
Steven F
SF
D000375:Aging / Q000276:immunology*; D000818:Animals; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008840:Microfilament Proteins; D009457:Neuroglia / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344
C100745:Aif1 protein, rat; D039481:CD11b Antigen; D002135:Calcium-Binding Proteins; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D008840:Microfilament Proteins