Master donor viruses A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 and derived reassortants used in live attenuated influenza vaccine (LAIV) do not display neurovirulent properties in a mouse model.
Demonstration of the absence of neurovirulent properties of reassortant viruses contained in live attenuated influenza vaccine (LAIV) is a regulatory requirement. A mouse model was used to detect neurovirulent properties of the cold-adapted, temperature-sensitive and attenuated influenza master donor viruses (MDVs) A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69 and derived reassortant influenza viruses. A/NWS/33 (H1N1), which is known to be neurovirulent in mice, was used as a positive control. Under conditions where the positive control virus induced symptoms of disease and showed viral replication in the upper respiratory tract as well as in the brain, replication of the influenza master donor viruses and reassortant influenza A and B viruses was limited to the upper respiratory tract where they were administered. None of the mice inoculated with MDVs or reassortant influenza viruses suffered from disease, and no virus or viral replication was observed in the brains of these mice. The results demonstrate the absence of neurovirulent properties of the MDVs and reassortant influenza viruses derived therefrom used in LAIV.
Voeten
J Theo M
JT
Nobilon Schering-Plough, Wim de Körverstraat 35, Boxmeer, The Netherlands. theo.voeten@sp.nobilon.com
Kiseleva
Irina V
IV
Glansbeek
Harrie L
HL
Basten
Stephanie M C
SM
Drieszen-van der Cruijsen
Sandra K M
SK
Rudenko
Larisa G
LG
van den Bosch
Han
H
Heldens
Jacco G M
JG
D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D002642:Chick Embryo; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006801:Humans; D053121:Influenza A Virus, H2N2 Subtype / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D009981:Influenza B virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D007251:Influenza, Human / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D051379:Mice; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014774:Virulence
D007252:Influenza Vaccines; D014613:Vaccines, Attenuated