Streptomyces youssoufiensis sp. nov., isolated from a Moroccan phosphate mine.
A novel actinomycete, strain X4(T), was isolated from a phosphate mine in Youssoufia, 100 km north of Marrakesh, Morocco. The taxonomic status of this strain was evaluated by a polyphasic approach. Strain X4(T) had white aerial mycelium with Rectiflexibiles spore chains bearing smooth-surfaced spores and did not produce diffusible pigments. Chemotaxonomic analysis showed that the cell wall of strain X4(T) contained LL-diaminopimelic acid and glycine. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that strain X4(T) belongs to the Group I streptomycetes, branching off next to Streptomyces ramulosus NRRL B-2714(T) and Streptomyces kasugaensis M338-M1(T). DNA-DNA relatedness and phenotypic data enabled strain X4(T) to be distinguished from the phylogenetically most closely related type strains. It is therefore proposed that strain X4(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces youssoufiensis sp. nov. is proposed; the type strain is X4(T) ( = CCMM B709(T)  = DSM 41920(T)).
Hamdali
H
H
Institut de Génétique et Microbiologie, Université Paris XI, 91405 Orsay, France.|Faculté de Sciences Semlalia, Université Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakesh, Morocco.
Virolle
M J
MJ
Institut de Génétique et Microbiologie, Université Paris XI, 91405 Orsay, France.
von Jan
M
M
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Spröer
C
C
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Klenk
H-P
HP
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Ouhdouch
Y
Y
Faculté de Sciences Semlalia, Université Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakesh, Morocco.
D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008906:Mining; D008969:Molecular Sequence Data; D009018:Morocco; D010710:Phosphates / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D010710:Phosphates; D012336:RNA, Ribosomal, 16S