[The effect of short hairpin RNA and DNAzyme gene therapy on latent membrane protein-1 expression in nasopharyngeal carcinoma].
OBJECTIVE
To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines.


METHODS
The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected.


RESULTS
The HNE1 cells expressing green fluorescence in combining group were significantly less than that in RNA interference group (P < 0.01); The inhibition efficiency of green fluorescence in combining group were 86. 31% Wp 88.88% ,which were higher than that in RNA interference group with 75.15%. The detection of LMP1 mRNA and proteins showed that combining group had a higher inhibition ability.


CONCLUSIONS
Combining gene therapy with pshLMP1 and DNAzyme is superior to pshLMP1 alone to inhibit LMP1 gene expression.
Department of Otolaryngology, Beijing Fengtai Hospital, Beijing,100071, China.
Yiqin
Y
Wang
Suling
S
Hong
Yucheng
Y
Yang
Di
D
Qian
Xia
X
Ke
D045744:Cell Line, Tumor; D021881:DNA, Catalytic / Q000627:therapeutic use*; D005091:Exons; D015967:Gene Expression Regulation, Viral; D015316:Genetic Therapy; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics*
D021881:DNA, Catalytic; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins