Mesoderm migration in Drosophila is a multi-step process requiring FGF signaling and integrin activity.
Migration is a complex, dynamic process that has largely been studied using qualitative or static approaches. As technology has improved, we can now take quantitative approaches towards understanding cell migration using in vivo imaging and tracking analyses. In this manner, we have established a four-step model of mesoderm migration during Drosophila gastrulation: (I) mesodermal tube formation, (II) collapse of the mesoderm, (III) dorsal migration and spreading and (IV) monolayer formation. Our data provide evidence that these steps are temporally distinct and that each might require different chemical inputs. To support this, we analyzed the role of fibroblast growth factor (FGF) signaling, in particular the function of two Drosophila FGF ligands, Pyramus and Thisbe, during mesoderm migration. We determined that FGF signaling through both ligands controls movements in the radial direction. Thisbe is required for the initial collapse of the mesoderm onto the ectoderm, whereas both Pyramus and Thisbe are required for monolayer formation. In addition, we uncovered that the GTPase Rap1 regulates radial movement of cells and localization of the beta-integrin subunit, Myospheroid, which is also required for monolayer formation. Our analyses suggest that distinct signals influence particular movements, as we found that FGF signaling is involved in controlling collapse and monolayer formation but not dorsal movement, whereas integrins are required to support monolayer formation only and not earlier movements. Our work demonstrates that complex cell migration is not necessarily a fluid process, but suggests instead that different types of movements are directed by distinct inputs in a stepwise manner.
McMahon
Amy
A
California Institute of Technology, Division of Biology MC 114-96, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Reeves
Gregory T
GT
Supatto
Willy
W
Stathopoulos
Angelike
A
D000818:Animals; D002465:Cell Movement; D048430:Cell Shape; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D054262:Gastrulation; D019012:Integrin beta1 / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D015398:Signal Transduction; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism
D029721:Drosophila Proteins; D019012:Integrin beta1; D016023:Integrins; C543524:pyr protein, Drosophila; C543523:ths protein, Drosophila; D005346:Fibroblast Growth Factors; D020827:rap1 GTP-Binding Proteins