T(2) determination of the J-coupled methyl protons of lipids: In vivo ilustration with tibial bone marrow at 3 T.
OBJECTIVE
To demonstrate how J-coupling modulations of the CH(3) lipid resonance can be minimized enabling a representative T(2) to be measured.


METHODS
Experiments were conducted on canola oil and in vivo on tibial bone marrow of four volunteers at 3 T. The T(2) of the CH(2) protons was measured with a standard point resolved spectroscopy (PRESS) sequence, whereas the T(2) of the CH(3) protons was determined with a PRESS sequence composed of narrow bandwidth refocusing pulses designed to exploit the chemical shift displacement effect and rewind the J-coupling evolution of the CH(3) protons in the desired voxel. Spectra were acquired at five echo times.


RESULTS
The narrow bandwidth PRESS sequence rewound the J-evolution of the CH(3) protons resulting in a T(2) curve that was well described by a monoexponential function. The mean T(2) of the bone marrow CH(3) protons was calculated to be 132.6 msec. The mean T(2) of the bone marrow CH(2) protons was estimated with a regular PRESS sequence to be 88.0 msec. The mean CH(2):CH(3) tibial bone marrow composition was estimated to be 12.0:1.


CONCLUSIONS
The presented technique permits the T(2) of the methyl protons of lipids to be determined with more accuracy by minimizing contributions from J-coupling.
Department of Medical Physics, Cross Cancer Institute, Edmonton, Alberta, Canada T6G 1Z2. ayahya@ualberta.ca
Atiyah
A
Yahya
B Gino
BG
Fallone
D001853:Bone Marrow / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019047:Phantoms, Imaging; D011522:Protons; D013977:Tibia / Q000473:pathology*
D008055:Lipids; D011522:Protons