Fucoxanthin supplementation improves plasma and hepatic lipid metabolism and blood glucose concentration in high-fat fed C57BL/6N mice.
This study investigated the effects of fucoxanthin isolated from marine plant extracts on lipid metabolism and blood glucose concentration in high-fat diet fed C57BL/6N mice. The mice were divided into high-fat control (HFC; 20% fat, w/w), low-fucoxanthin (low-Fxn; HFC+0.05% Fxn, w/w) and high-fucoxanthin (high-Fxn; HFC+0.2% Fxn, w/w) groups. Fxn supplementation significantly lowered the concentration of plasma triglyceride with a concomitant increase of fecal lipids in comparison to the HFC group. Also, the hepatic lipid contents were significantly lowered in the Fxn supplemented groups which seemed to be due to the reduced activity of the hepatic lipogenic enzymes, glucose-6-phosphate dehydrogenase, malic enzyme, fatty acid synthase and phosphatidate phosphohydrolase and the enhanced activity of beta-oxidation. Plasma high-density lipoprotein cholesterol concentrations and its percentage were markedly elevated by Fxn supplementation. Activities of two key cholesterol regulating enzymes: 3-hydroxy-3-methylglutaryl coenzyme A reductase and acyl coenzyme A: cholesterol acyltransferase, were significantly suppressed by Fxn regardless of the dosage. Relative mRNA expressions of acyl-coA oxidase 1, palmitoyl (ACOX1) and peroxisome proliferators activated receptor alpha (PPARalpha) and gamma (PPARgamma) were significantly altered by Fxn supplementation in the liver. Fxn also lowered blood glucose and HbA(1c) levels along with plasma resistin and insulin concentrations. These results suggest that Fxn supplementation plays a beneficial role in not only regulating the plasma and hepatic lipids metabolism but also for blood glucose-lowering action in high-fat fed mice.
Department of Food Science and Nutrition, Center for Food & Nutritional Genomics, Kyungpook National University, Daegu 702-701, Republic of Korea.
Myoung-Nam
MN
Woo
Seon-Min
SM
Jeon
Hye-Jin
HJ
Kim
Mi-Kyung
MK
Lee
Su-Kyung
SK
Shin
Young Chul
YC
Shin
Yong-Bok
YB
Park
Myung-Sook
MS
Choi
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004041:Dietary Fats; D019587:Dietary Supplements; D004435:Eating; D005934:Glucagon / Q000097:blood; D006441:Hemoglobin A / Q000378:metabolism; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000139:chemically induced / Q000178:diet therapy*; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D015430:Weight Gain; D024341:Xanthophylls / Q000302:isolation & purification / Q000627:therapeutic use*
D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D010936:Plant Extracts; D012333:RNA, Messenger; D024341:Xanthophylls; C025164:fucoxanthin; D005934:Glucagon; D006441:Hemoglobin A