Stereotyped patterns of B-cell receptor in splenic marginal zone lymphoma.
Antigen stimulation may be important for splenic marginal zone lymphoma pathogenesis. To address this hypothesis, the occurrence of stereotyped B-cell receptors was investigated in 133 SMZL (26 HCV+) compared with 4,414 HCDR3 sequences from public databases. Sixteen SMZL (12%) showed stereotyped BCR; 7 of 86 (8%) SMZL sequences retrieved from public databases also belonged to stereotyped HCDR3 subsets. Three categories of subsets were identified: i) "SMZL-specific subsets" (n=5), composed only of 12 SMZL (9 HCV-from our series); ii) "Non-Hodgkin's lymphoma-like subsets" (n=5), comprising 5 SMZL (4 from our series) clustering with other indolent lymphomas; iii) "CLL-like subsets" (n=6), comprising 6 SMZL (3 from our series) that belonged to known CLL subsets (n=4) or clustered with public CLL sequences. Immunoglobulin 3D modeling of 3 subsets revealed similarities in antigen binding regions not limited to HCDR3. Overall, data suggest that the pathogenesis of splenic marginal zone lymphoma may involve also HCV-unrelated epitopes or an antigenic trigger common to other indolent lymphomas.
Zibellini
Silvia
S
Division of Hematology, Department of Oncohematology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Viale Golgi 19, Pavia, Italy.
Capello
Daniela
D
Forconi
Francesco
F
Marcatili
Paolo
P
Rossi
Davide
D
Rattotti
Sara
S
Franceschetti
Silvia
S
Sozzi
Elisa
E
Cencini
Emanuele
E
Marasca
Roberto
R
Baldini
Luca
L
Tucci
Alessandra
A
Bertoni
Francesco
F
Passamonti
Francesco
F
Orlandi
Ester
E
Varettoni
Marzia
M
Merli
Michele
M
Rizzi
Silvia
S
Gattei
Valter
V
Tramontano
Anna
A
Paulli
Marco
M
Gaidano
Gianluca
G
Arcaini
Luca
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens; D000939:Epitopes; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000209:etiology* / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013160:Splenic Neoplasms / Q000209:etiology* / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D011947:Receptors, Antigen, B-Cell