Isolation of nucleoli.
Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit.
Hacot
Sabine
S
Université de Lyon, Lyon, France.
Coute
Yohann
Y
Belin
Stéphane
S
Albaret
Marie Alexandra
MA
Mertani
Hichem C
HC
Sanchez
Jean-Charles
JC
Rosa-Calatrava
Manuel
M
Diaz
Jean-Jacques
JJ
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002458:Cell Fractionation / Q000379:methods*; D002466:Cell Nucleolus / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans