Determining the yield and quality of purified RNA.
After any RNA purification by any method, it is necessary to ascertain both the amount (yield) and the integrity (quality) of the RNA obtained. This is essential for any subsequent analysis and critical for any comparative analyses. This article discusses several methods for RNA quantitation and provides tips for each.
Rio
Donald C
DC
Ares
Manuel
M
Hannon
Gregory J
GJ
Nilsen
Timothy W
TW
D001671:Biochemistry / Q000379:methods*; D015152:Blotting, Northern; D004587:Electrophoresis, Agar Gel; D005470:Fluorometry; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification* / Q000592:standards*; D012331:RNA, Fungal / Q000302:isolation & purification / Q000592:standards; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013053:Spectrophotometry
D012331:RNA, Fungal; D012313:RNA