Rhizobium kunmingense sp. nov., isolated from rhizosphere soil of Camptotheca acuminata Decne.
Strain LXD30(T) was isolated from rhizosphere soil of a plant of the species Camptotheca acuminata Decne which is native to warm, humid stream banks in southern China. Analysis of the 16S rRNA gene sequence revealed that the Gram-negative, rod-shaped bacterium fell within the realm of the genus Rhizobium and was most closely related to Rhizobium huautlense SO2(T) (96.4% sequence similarity) and Rhizobium cellulosilyticum LMG 23642(T) (96.4%). The isolate grew optimally at pH7.0 and 25-28 degrees C in the presence of 0-1% (w/v) NaCl. Major fatty acids were C16:0 (17.5%) and summed feature 7 (C18:1omega7c/omega9t/omega12t, 58.3%). Unequivocally low 16S rRNA (<97%), recA (<92%) and atpD (<90%) gene sequence similarities to all existing species of the genus and phenotypic characteristics all suggested that strain LXD30(T) (=KCTC 22609(T)=CGMCC 1.8903(T)) represents a novel Rhizobium species, for which the name Rhizobium kunmingense sp. nov. is proposed.
Shen
Lei
L
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China.
Zheng
Long-Peng
LP
Liu
Huan
H
Liu
Rui
R
Zhang
Ke-Yun
KY
Lai
Ren
R
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D029769:Camptotheca / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S