Molecular characterization of Mycobacterium intracellulare-related strains based on the sequence analysis of hsp65, internal transcribed spacer and 16S rRNA genes.
We investigated the molecular epidemiological features of 94 Mycobacterium intracellulare-related strains, isolated from Korean patients, using sequence analysis targeting 3 independent chronometer molecules, hsp65, the internal transcribed spacer 1 region and the 16S rRNA gene. By collective consideration of these three gene-based approaches, the 94 strains were divided into 5 groups (INT1, INT2, INT3, INT4 and INT5). The frequencies of genotype INT1, 2, 3, 4 and 5 in the 94 isolates were 57.4 % (54), 27.7 % (26), 6.4 % (6), 5.3 % (5) and 3.2 % (3), respectively. When correlations between genotypes and clinical parameters (age, sex, radiological type and the presence of a cavity) were analysed in 78 patients with non-tuberculous mycobacteria pulmonary diseases, no relationships were observed with respect to age, sex and radiological type, but genotype and the presence of a cavity tended to be related (P=0.051).
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Joo-Hee
JH
Park
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
Tae-Sun
TS
Shim
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Seung-Ae
SA
Lee
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Hyungki
H
Lee
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
In-Kyung
IK
Lee
Department of Microbiology, School of Medicine, Chung-Ang University, Seoul, Republic of Korea.
Kijeong
K
Kim
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Yoon-Hoh
YH
Kook
Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.|Department of Microbiology and Immunology, Cancer Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Bum-Joon
BJ
Kim
D000368:Aged; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005260:Female; D005838:Genotype; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics*; D015270:Mycobacterium avium-intracellulare Infection / Q000453:epidemiology / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D021903:DNA, Ribosomal Spacer; D006360:Heat-Shock Proteins; D012336:RNA, Ribosomal, 16S