Foot-and-mouth disease virus 2C is a hexameric AAA+ protein with a coordinated ATP hydrolysis mechanism.
Foot-and-mouth disease virus (FMDV), a positive sense, single-stranded RNA virus, causes a highly contagious disease in cloven-hoofed livestock. Like other picornaviruses, FMDV has a conserved 2C protein assigned to the superfamily 3 helicases a group of AAA+ ATPases that has a predicted N-terminal membrane-binding amphipathic helix attached to the main ATPase domain. In infected cells, 2C is involved in the formation of membrane vesicles, where it co-localizes with viral RNA replication complexes, but its precise role in virus replication has not been elucidated. We show here that deletion of the predicted N-terminal amphipathic helix enables overexpression in Escherichia coli of a highly soluble truncated protein, 2C(34-318), that has ATPase and RNA binding activity. ATPase activity was abrogated by point mutations in the Walker A (K116A) and B (D160A) motifs and Motif C (N207A) in the active site. Unliganded 2C(34-318) exhibits concentration-dependent self-association to yield oligomeric forms, the largest of which is tetrameric. Strikingly, in the presence of ATP and RNA, FMDV 2C(34-318) containing the N207A mutation, which binds but does not hydrolyze ATP, was found to oligomerize specifically into hexamers. Visualization of FMDV 2C-ATP-RNA complexes by negative stain electron microscopy revealed hexameric ring structures with 6-fold symmetry that are characteristic of AAA+ ATPases. ATPase assays performed by mixing purified active and inactive 2C(34-318) subunits revealed a coordinated mechanism of ATP hydrolysis. Our results provide new insights into the structure and mechanism of picornavirus 2C proteins that will facilitate new investigations of their roles in infection.
Sweeney
Trevor R
TR
Biophysics Section, Blackett Laboratory, Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom.
Cisnetto
Valentina
V
Bose
Daniel
D
Bailey
Matthew
M
Wilson
Jon R
JR
Zhang
Xiaodong
X
Belsham
Graham J
GJ
Curry
Stephen
S
D000255:Adenosine Triphosphate / Q000737:chemistry*; D020816:Amino Acid Motifs; D002352:Carrier Proteins / Q000737:chemistry* / Q000502:physiology*; D020134:Catalytic Domain; D004926:Escherichia coli / Q000378:metabolism; D029544:Foot-and-Mouth Disease Virus / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D009154:Mutation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry; D012367:RNA, Viral / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000502:physiology*; D014764:Viral Proteins / Q000737:chemistry
D002352:Carrier Proteins; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D012313:RNA; D000255:Adenosine Triphosphate; C076023:2C protein, viral