A new pre-column derivatization method for determination of gabapentin in human serum by HPLC using UV detection.
A high-performance liquid chromatography method using UV detection for determination of gabapentin in human serum has been developed. Serum was deproteinized with acetonitrile, and supernatant was evaporated. Derivatization was performed using 4-nitrophenylisothiocyanate, and separation was achieved on a C(18) column with detection at 335 nm. The method was linear in the 0.12-6.0 microg/mL range. No interference was found from endogenous amino acids. The method is suitable for routine therapeutic drug monitoring and for pharmacokinetic studies.
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences.
Mohsen
M
Amini
Mohammad-Reza
MR
Rouini
Alireza
A
Asad-Paskeh
Abbas
A
Shafiee
D000588:Amines / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000097:blood* / Q000737:chemistry; D000077206:Gabapentin; D006801:Humans; D044967:Serum / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000097:blood* / Q000737:chemistry
D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin