Isolation and quantitative analysis of peroxynitrite scavengers from Artemisia princeps var. orientalis.
Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO(-))-scavenging effect (IC(50), 0.26 microg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC(50) values of compounds 1 and 2 were 0.18 +/- 0.01 microg/mL and 0.12 +/- 0.00 microg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).
Department of Pharmaceutical Engineering, Sangji University, Wonju, Korea.
Agung
A
Nugroho
Kang Ro
KR
Lee
Md Badrul
MB
Alam
Jae Sue
JS
Choi
Hee-Juhn
HJ
Park
D019306:Artemisia / Q000737:chemistry*; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D010396:Penicillamine / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D026361:Reactive Nitrogen Species / Q000037:antagonists & inhibitors
C552116:3,5-di-O-caffeoyl-muco-quinic acid; D002109:Caffeic Acids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D026361:Reactive Nitrogen Species; C552117:methyl 4,5-di-O-caffeoylquinate; D011801:Quinic Acid; D030421:Peroxynitrous Acid; D010396:Penicillamine