Parallel optical monitoring of visual signal propagation from the photoreceptors to the inner retina layers.
Understanding of visual signal processing can benefit from simultaneous measurement of different types of retinal neurons working together. In this Letter, we demonstrate that intrinsic optical signal (IOS) imaging of frog retina slices allows simultaneous observation of stimulus-evoked responses propagating from the photoreceptors to the inner neurons. High-resolution imaging revealed robust IOSs at the photoreceptor, the inner plexiform, and the ganglion cell layers. While IOSs of the photoreceptor layer were mainly confined to the area directly stimulated by the visible light, IOSs of the inner retinal layers spread from the stimulus site into relatively large areas with a characteristic near-to-far time course.
Li
Yi-Chao
YC
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Strang
Christianne
C
Amthor
Franklin R
FR
Liu
Lei
L
Li
Yang-Guo
YG
Zhang
Qiu-Xiang
QX
Keyser
Kent
K
Yao
Xin-Cheng
XC
D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008029:Lighting / Q000295:instrumentation*; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology* / Q000502:physiology*; D011898:Ranidae; D042261:Retinoscopes; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*