Distributions of PCNA and Cas-3 in rat uterus during early pregnancy.
The aim of present study was to determine the distributions of proliferating cell nuclear agent (PCNA) and Cas-pase-3 (Cas-3) and their possible roles in implantation and decidualization during early pregnancy at immunohistochemical level. The tissue samples from pregnant animals between gestational days 1-5 were incubated by PCNA and Cas-3 antibodies and the obtained results were evaluated quantitatively. It was observed that PCNA immunoreactivity in uterine luminal epithelium and glandular epithelium reduced as from day 2 of gestation and disappeared as from day 4 of gestation. PCNA staining intensity in stromal cells and myometrium increased gradually with progressing gestation. While Cas-3 immunoreactivity was strongly detected in luminal and glandular epithelium throughout the whole gestational period, its reactivity markedly increased as from day 3 of gestation. In conclusion, it may suggest that the blastocyst implantation induces the uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus.
Oner
Hakan
H
Department of Histology and Embryology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey. hakanoner@mehmetakif.edu.tr
Oner
Jale
J
Demir
Ramazan
R
D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology; D005260:Female; D009215:Myometrium / Q000166:cytology / Q000201:enzymology; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000166:cytology / Q000201:enzymology*
D018809:Proliferating Cell Nuclear Antigen; D053148:Caspase 3