Development of MHC class I and II B primers in common carp and its molecular characterization.
The major histocompatibility complex (MHC) has an important role in immune response and is known as the most polymorphic locus in vertebrates. We developed three pairs of polymerase chain reaction primers of the alpha-2 domain (exon 3) of MHC class I and the beta-2 (exon 3) and beta-3 domains (exon 4) of MHC class II B gene in the German mirror common carp (Cyprinus carpio L.). We analyzed the three loci in a population of 65 individuals that had suffered the serious disease of gill rot. Five to six variable nucleotide sites and two to six variable amino acid sites (71.43%) were detected in the exon sequence of the sampled populations, indicating that many of them corresponded to amino acids involved in antigen recognition. Deviation from Hardy-Weinberg equilibrium and linkage disequilibrium were differentially found in some loci, which will be important for further study of disease resistance/susceptibility and population evolution.
Jia
Zhiying
Z
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China.
Chi
Xifeng
X
Li
Chitao
C
Shi
Lianyu
L
D000818:Animals; D002347:Carps / Q000235:genetics* / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D005858:Germany; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II