Synthetic polymer coatings for long-term growth of human embryonic stem cells.
We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA.
Luis G
LG
Villa-Diaz
Himabindu
H
Nandivada
Jun
J
Ding
Naiara C
NC
Nogueira-de-Souza
Paul H
PH
Krebsbach
K Sue
KS
O'Shea
Joerg
J
Lahann
Gary D
GD
Smith
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D011108:Polymers / Q000494:pharmacology*; D013997:Time Factors
D020099:Coated Materials, Biocompatible; D003470:Culture Media; D011108:Polymers