Immunohistochemical mapping of histamine, dopamine, and serotonin in the central nervous system of the copepod Calanus finmarchicus (Crustacea; Maxillopoda; Copepoda).
Calanoid copepods constitute an important group of marine planktonic crustaceans that often dominate the metazoan biomass of the world's oceans. In proportion to their ecological importance, little is known about their nervous systems. We have used immunohistochemical techniques in a common North Atlantic calanoid to localize re-identifiable neurons that putatively contain the biogenic amines histamine, dopamine, and serotonin. We have found low numbers of such cells and cell groups (approximately 37 histamine pairs, 22 dopamine pairs, and 12 serotonin pairs) compared with those in previously described crustaceans. These cells are concentrated in the anterior part of the central nervous system, the majority for each amine being located in the three neuromeres that constitute the brain (protocerebrum, deutocerebrum, and tritocerebrum). Extensive histamine labeling occurs in several small compact protocerebral neuropils, three pairs of larger, more posterior, paired, dense neuropils, and one paired diffuse tritocerebral neuropil. The most concentrated neuropil showing dopamine labeling lies in the putative deutocerebrum, associated with heavily labeled commissural connections between the two sides of the brain. The most prominent serotonin neuropil is present in the anterior medial part of the brain. Tracts of immunoreactive fibers of all three amines are prominent in the cephalic region of the nervous system, but some projections into the most posterior thoracic regions have also been noted.
Hartline
Daniel K
DK
Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center (PBRC), University of Hawaii at Manoa, Honolulu, HI 96822, USA. danh@pbrc.hawaii.edu
Christie
Andrew E
AE
D000818:Animals; D001369:Axons / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D003445:Crustacea / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D007150:Immunohistochemistry; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D006632:Histamine; D004298:Dopamine