Rheinheimera nanhaiensis sp. nov., isolated from marine sediments, and emended description of the genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007.
A Gram-negative, facultatively aerobic, oxidase- and catalase-positive, rod-shaped bacterium, designated strain E407-8(T), was isolated from a sediment sample from the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain E407-8(T) was affiliated with the genus Rheinheimera, sharing the highest sequence similarity with Rheinheimera pacifica KMM 1406(T) (97.5 %) and Rheinheimera aquimaris SW-353(T) (97.4 %) and showing less than 97 % sequence similarity to the type strains of other recognized Rheinheimera species. Levels of DNA-DNA relatedness of strain E407-8(T) to R. pacifica DSM 17616(T) and R. aquimaris JCM 14331(T) were 25.2 % (25.3 % in the duplicate measurement) and 9.4 % (6.5 %), respectively. The bacterium could grow at 10-48 °C (optimum 37 °C) and in the presence of 0-8 % (w/v) NaCl (optimum 0.5-2.5 %). The major cellular fatty acids of strain E407-8(T) were summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), C(17 : 1)ω8c, C(16 : 0) and C(18 : 1)ω7c. The predominant respiratory quinone was ubiquinone Q-8. The DNA G+C content was 51.0 mol%. Based on the results of our polyphasic taxonomic study, strain E407-8(T) represents a novel species in the genus Rheinheimera, for which the name Rheinheimera nanhaiensis sp. nov. is proposed. The type strain is E407-8(T) ( = CCTCC AB 209089(T)  = KACC 14030(T)). An emended description of the genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007 is also proposed.
College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.|The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Hui-Juan
HJ
Li
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Xi-Ying
XY
Zhang
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Yan-Jiao
YJ
Zhang
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Ming-Yang
MY
Zhou
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Zhao-Ming
ZM
Gao
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Xiu-Lan
XL
Chen
Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, PR China.
Hong-Yue
HY
Dang
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Yu-Zhong
YZ
Zhang
D002841:Chromatiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S