Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.
DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.
Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Ryuji
R
Igarashi
Tomomi
T
Sakai
Hideyuki
H
Hara
Takeshi
T
Tenno
Toshiaki
T
Tanaka
Hidehito
H
Tochio
Masahiro
M
Shirakawa
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D008958:Models, Molecular; D009865:Oocytes; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels; D025801:Ubiquitin / Q000737:chemistry; D014982:Xenopus laevis
D011506:Proteins; D013113:Spin Labels; D025801:Ubiquitin