Application of DNA barcoding technique in avian influenza virus surveillance of wild bird habitats in Korea and Mongolia.
In a previous study, we optimized DNA barcoding techniques for avian influenza virus (AIV) isolation and host identification, using fecal samples from wild birds, for high-throughput surveillance of migratory waterfowls. In the present study, we surveyed AIV in Mongolia during the breeding season and, subsequently, in Korea in winter, to compare prevalent AIV subtypes and hosts using DNA barcoding. In Korea, H4 and H5 subtypes were the most abundantly detected HA subtypes, and most AIVs were isolated from the major population (mallards, Anas platyrhynchos) of wild bird habitats. On the other hand, in Mongolia, H3 and H4 subtypes were the most abundantly detected HA subtypes, and most AIVs were isolated from a small population of wild bird habitats that were not visible at the sampling site. In conclusion, AIV isolation using fecal samples, accompanied with DNA barcoding techniques as a host bird species identification tool, could be useful for monitoring major and minor populations of wild bird habitats. Further, continuous, and large-scale surveillance could be helpful for understanding the AIV epidemiology, evolution, and ecology in wild waterfowl.
Lee
Dong-Hun
DH
Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 143-701 Seoul, Korea.
Lee
Hyun-Jeong
HJ
Lee
Yu-Na
YN
Lee
Youn-Jeong
YJ
Jeong
Ok-Mi
OM
Kang
Hyun-Mi
HM
Kim
Min-Chul
MC
Kwon
Ji-Sun
JS
Kwon
Jun-Hun
JH
Lee
Joong-Bok
JB
Park
Seung-Yong
SY
Choi
In-Soo
IS
Song
Chang-Seon
CS
D000818:Animals; D000835:Animals, Wild; D001717:Birds; D004279:DNA, Viral / Q000032:analysis*; D017753:Ecosystem; D009980:Influenza A virus / Q000235:genetics; D005585:Influenza in Birds / Q000453:epidemiology* / Q000821:virology; D007723:Korea / Q000453:epidemiology; D008986:Mongolia / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000379:methods*
D004279:DNA, Viral