Isolation of Brazilian marine fungi capable of growing on DDD pesticide.
The fungi Aspergillus sydowii Ce15, Aspergillus sydowii Ce19, Aspergillus sydowii Gc12, Bionectria sp. Ce5, Penicillium miczynskii Gc5, Penicillium raistrickii Ce16 and Trichoderma sp. Gc1, isolated from marine sponges Geodia corticostylifera and Chelonaplysylla erecta, were evaluated for their ability to grow in the presence of DDD pesticide. Increasing concentrations of DDD pesticide, i.e., 5.0 mg (1.56 × 10⁻¹² mmol), 10.0 mg (3.12 × 10⁻²) mmol) and 15.0 mg (4.68 × 10⁻² mmol) in solid and liquid culture media were tested. The fungi Trichoderma sp. Gc1 and Penicillium miczynskii Gc5 were able to grow in the presence of up to 15.0 mg of DDD, suggesting their potential for biodegradation. A 100% degradation of DDD was attained in liquid culture medium when Trichoderma sp. Gc1 was previously cultivated for 5 days and supplemented with 5.0 mg of DDD in the presence of hydrogen peroxide. However, the quantitative analysis showed that DDD was accumulated on mycelium and biodegradation level reached a maximum value of 58% after 14 days.
Instituto de Química de São Carlos, Universidade de São Paulo, SP, Brazil.
Scarlet Nere
SN
Ortega
Marcia
M
Nitschke
Ana Maria
AM
Mouad
Maria Diva
MD
Landgraf
Maria Olímpia Oliveira
MO
Rezende
Mirna Helena Regali
MH
Seleghim
Lara Durães
LD
Sette
André Luiz Meleiro
AL
Porto
D000818:Animals; D001673:Biodegradation, Environmental; D001938:Brazil; D003470:Culture Media / Q000378:metabolism; D003632:Dichlorodiphenyldichloroethane / Q000378:metabolism*; D005658:Fungi / Q000302:isolation & purification* / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D011161:Porifera / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*
D003470:Culture Media; D010575:Pesticides; D003632:Dichlorodiphenyldichloroethane