Osteogenic protein-1 associated with mesenchymal stem cells promote bone allograft integration.
Limited incorporation and modest bone remodeling can cause allograft failure. We investigated whether mesenchymal stem cells (MSCs) and osteogenic protein-1 (OP-1) can improve allograft integration. A 3-cm full-size intercalary bone defect was created in the mid-diaphysis of the metatarsal bone of the sheep and it was replaced with an allograft alone (control group), or with MSCs (MSC group), OP-1 (OP-1 group), or MSCs and OP-1 (MSC + OP-1 group). Radiographic results showed a faster and complete integration of the allograft in the MSC + OP-1 group. Histology demonstrated that the amount of new bone was significantly greater inside the graft and a longer vessel penetration in the MSC + OP-1 group than in others. Mechanical strength of the allograft was not compromised by the high rate of bone remodeling. These results demonstrated that the association of MSCs and OP-1 improve bone allograft integration promoting an almost complete bone restoring.
Bone Regeneration Laboratory, Department of Muskulo-Skeletal Oncology, Research Institute Codivilla-Putti, Rizzoli Orthopaedic Institute, Bologna, Italy. claudia.dibella@ior.it
Claudia
C
Di Bella
Nicolò Nicoli
NN
Aldini
Enrico
E
Lucarelli
Barbara
B
Dozza
Tommaso
T
Frisoni
Lucia
L
Martini
Milena
M
Fini
Davide
D
Donati
D000818:Animals; D001696:Biomechanical Phenomena; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D016025:Bone Transplantation / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008682:Metatarsal Bones / Q000378:metabolism / Q000601:surgery; D010012:Osteogenesis / Q000502:physiology*; D012756:Sheep; D014184:Transplantation, Homologous
D055419:Bone Morphogenetic Protein 7