The analgesic effect and mechanism of the combination of sodium ferulate and oxymatrine.
Sodium ferulate (SF) and Oxymatrine (OMT) were compounds extracted from Chinese herbs, and have been used in clinical treatment of heart and hepatic diseases, respectively, in China for many years. The objective of this study was to examine the analgesic effect and the mechanism of the combined treatment of SF and OMT. Using the animal pain models by applying Acetic Acid Writhing Test and Formalin Test, the combination of SF and OMT showed significant analgesic effect in dose-dependent manner. In vitro, the combined treatment inhibited the increase in intracellular calcium concentration evoked by capsaicin in the dorsal root ganglion neurons. Importantly, a synergistic inhibitory effect of SF and OMT on the capsaicin-induced currents was demonstrated by whole-cell patch-clamp. Our results suggest that SF and OMT cause significant analgesic effect which maybe related to the synergistic inhibition of transient receptor potential vanilloid-1.
School of Pharmacy, Yantai University, Laishan District, Yantai, Shandong, China.
Hanqing
H
Liu
Yan
Y
Sun
Yan
Y
Gao
Fangfang
F
Chen
MingBo
M
Xu
Zhifeng
Z
Liu
D019342:Acetic Acid / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology / Q000627:therapeutic use; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D003373:Coumaric Acids / Q000494:pharmacology / Q000627:therapeutic use; D005557:Formaldehyde / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D010147:Pain Measurement; D018408:Patch-Clamp Techniques; D011807:Quinolizines / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000378:metabolism; D000093842:Matrines
D019342:Acetic Acid; D000470:Alkaloids; D000700:Analgesics; D000889:Anti-Arrhythmia Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003373:Coumaric Acids; D005557:Formaldehyde; D011807:Quinolizines; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C494484:TRPV1 receptor; C493922:Trpv1 protein, rat; C037573:oxymatrine; D000093842:Matrines; C004999:ferulic acid