Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains.
BACKGROUND
The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes.


METHODS
Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed. Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study.


RESULTS
The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 microg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 microg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 microg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed.


CONCLUSIONS
The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents.
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. asad.k@rediffmail.com
Rosina
R
Khan
Mohammad
M
Zakir
Sadul H
SH
Afaq
Abdul
A
Latif
Asad U
AU
Khan
D019661:Apiaceae; D001419:Bacteria / Q000187:drug effects*; D024901:Drug Resistance, Multiple, Bacterial; D026141:Drug Resistance, Multiple, Fungal; D005658:Fungi / Q000187:drug effects*; D020939:Zingiber officinale; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D029862:Prosopis
D010936:Plant Extracts