Physicochemical and biological properties of nano-hydroxyapatite-reinforced aliphatic polyurethanes membranes.
Polymer nano-composite membranes, based on aliphatic biodegradable polyurethane (PU) elastomers and nano-hydroxyapatite (n-HA), were prepared by solvent casting and freeze-drying. The PU matrix was synthesized from 4,4'-dicyclohexylmethane diisocyanate (H(12) MDI), poly(ethylene glycol) (PEG), castor oil (CO) and 1,4-butandiol (BDO). The n-HA/PU membranes were characterized by SEM, XRD, IR, TG, mechanical test and in vitro biocompatibility. The results revealed that incorporation of 30 wt% n-HA into the PU matrix increased the tensile strength nearly by 186% and the elongation-at-break by 107% compared to pure PU. The addition of n-HA had the slight positive effect on the thermal stability of PU. Cell culture and MTT assays showed that the incorporation of n-HA into the PU matrix provided a favourable environment for initial cell adhesion, maintained cell viability and cell proliferation. These results suggested that the n-HA/PU composite membrane might be a prospective biodegradable guided bone regeneration (GBR) membrane for future applications.
The Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, P. R. China.
Haohuai
H
Liu
Li
L
Zhang
Jidong
J
Li
Qin
Q
Zou
Yi
Y
Zuo
Weidong
W
Tian
Yubao
Y
Li
D001861:Bone Regeneration; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
D008567:Membranes, Artificial; D011140:Polyurethanes; D017886:Durapatite