Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway.
Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10l1 mutant testes causes loss of DNA methylation and subsequent derepression of retrotransposons in germ cells. The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins.
Zheng
Ke
K
Center for Animal Transgenesis and Germ Cell Research and Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA.
Xiol
Jordi
J
Reuter
Michael
M
Eckardt
Sigrid
S
Leu
N Adrian
NA
McLaughlin
K John
KJ
Stark
Alexander
A
Sachidanandam
Ravi
R
Pillai
Ramesh S
RS
Wang
Peijing Jeremy
PJ
D000818:Animals; D060565:Argonaute Proteins; D001483:Base Sequence; D018797:Cell Cycle Proteins; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D005298:Fertility; D008297:Male; D008540:Meiosis; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D011506:Proteins / Q000378:metabolism; D020365:RNA Helicases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D018626:Retroelements / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism
D060565:Argonaute Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; C448522:Piwil1 protein, mouse; C448521:Piwil2 protein, mouse; D011506:Proteins; D034741:RNA, Small Interfering; D018626:Retroelements; D017411:Ribonucleoproteins, Small Nuclear; C488681:Tdrd1 protein, mouse; C477006:Mov10l1 protein, mouse; D020365:RNA Helicases