Musculoskeletal response to whole-body vibration during fracture healing in intact and ovariectomized rats.
This study investigated the effect of vibration on bone healing and muscle in intact and ovariectomized rats. Thirty ovariectomized (at 3 months of age) and 30 intact 5-month old female Sprague-Dawley rats underwent bilateral metaphyseal osteotomy of tibia. Five days later, half of the ovariectomized and of the intact rats were exposed to whole-body vertical vibration (90 Hz, 0.5 mm, 4 x g acceleration) for 15 min twice a day during 30 days. The other animals did not undergo vibration. After decapitation of rats, one tibia was used for computed tomographic, biomechanical, and histological analyses; the other was used for gene expression analyses of alkaline phosphatase (Alp), osteocalcin (Oc), tartrate-resistant acid phosphatase 1, and insulinlike growth factor 1. Serum Alp and Oc were measured. Mitochondrial activity, fiber area and distribution, and capillary densities were analyzed in M. gastrocnemius and M. longissimus. We found that vibration had no effect on body weight and food intake, but it improved cortical and callus densities (97 vs. 99%, 72 vs. 81%), trabecular structure (9 vs. 14 trabecular nodes), blood supply (1.7 vs. 2.1 capillaries/fiber), and oxidative metabolism (17 vs. 23 pmol O(2)/s/mg) in ovariectomized rats. Vibration generally increased muscle fiber size. Tibia biomechanical properties were diminished after vibration. Oc gene expression was higher in vibrated rats. Serum Alp was increased in ovariectomized rats. In ovariectomized rats, vibration resulted in an earlier bridging; in intact rats, callus bridging occurred later after vibration. The chosen vibration regimen (90 Hz, 0.5 mm, 4 x g acceleration, 15 min twice a day) was effective in improving musculoskeletal tissues in ovariectomized rats but was not optimal for fracture healing.
Department of Trauma Surgery and Reconstructive Surgery, University of Goettingen, Robert-Koch St. 40, 37075 Goettingen, Germany.
Ewa K
EK
Stuermer
Marina
M
Komrakova
Carsten
C
Werner
Michael
M
Wicke
Leila
L
Kolios
Stephan
S
Sehmisch
Mohammad
M
Tezval
Clara
C
Utesch
Orzala
O
Mangal
Sebastian
S
Zimmer
Christian
C
Dullin
Klaus M
KM
Stuermer
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001835:Body Weight / Q000502:physiology; D015519:Bone Density / Q000502:physiology; D001842:Bone and Bones / Q000473:pathology; D002146:Bony Callus / Q000502:physiology; D005260:Female; D017102:Fracture Healing / Q000502:physiology*; D007527:Isoenzymes / Q000032:analysis; D018482:Muscle, Skeletal / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000473:pathology; D014732:Vibration / Q000627:therapeutic use*
D007527:Isoenzymes; D015675:Osteocalcin; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase