Idesolide, an isolate of Idesia polycarpa, inhibits apoptosis through induction of intracellular heat shock protein 70 in C2C12 muscle cells.
Muscle disorders, such as muscular dystrophy, are associated with an increase in oxidative stress. Proposed treatments for muscular dystrophy, some in clinical trials, include gene therapy and muscle cell transplantation. In this study, we investigated the effects of idesolide, isolated from the fruits of Idesia polycarpa, on changes that occur in muscle disuse atrophy. We noted protective effects on oxidative stress response and HSP70 regulation. Pre-treatment with idesolide for 24 h maintained cell viability and decreased apoptosis in H(2)O(2)-treated C(2)C(12) muscle cells. The idesolide pretreatment also increased intracellular HSP70 protein. Our results suggest that idesolide inhibits cell death through induction of HSP70 in C(2)C(12) muscle cells. This work is the first to report that idesolide can regulate the decrease in HSP70 that occurs during skeletal muscle atrophy.
Division of Biological Science and Technology, College of Science and Technology, Institute of Biomaterials, Yonsei University, Wonju 220-710, Korea.
Min-Ho
MH
Jung
Jung-Min
JM
Yoo
Yeo-Jin
YJ
Kang
Hyoung Woo
HW
Lee
Seung Hyun
SH
Kim
Sang Hyun
SH
Sung
Yong-Jin
YJ
Lee
Inho
I
Choi
Tack-Joong
TJ
Kim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006861:Hydrogen Peroxide; D051379:Mice; D032342:Muscle Cells / Q000187:drug effects*; D009133:Muscular Atrophy / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D031308:Salicaceae / Q000737:chemistry*; D013141:Spiro Compounds / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts; D013141:Spiro Compounds; C510160:idesolide; D006861:Hydrogen Peroxide