Embryo-fetal erythroid megaloblasts in the human coelomic cavity.
The coelomic cavity is part of the extraembryonic mesoderm, surrounding amniotic cavity, embryo, and yolk sac in the early gestation. It is now believed to represent an important transfer interface and a reservoir of nutrients for the embryo. Coelocentesis by ultrasound-guided transvaginal puncture offers an easier access to the early human embryo, from 28 days post-fertilization. However, despite some studies about its biochemical composition being reported, our knowledge about the presence of cellular elements and their quality in this compartment are still limited. Here we studied human coelomic fluids sampled from 6.6 (48 days) to 10 weeks of gestation, demonstrating the presence of functional embryonic erythroid precursors, that is, megaloblasts in the coelomic cavity. The ease of access of the coelomic cavity could allow the development of novel strategies for diagnostic or therapeutic purposes by ultrasound imaging and ultrasound-guided puncture.
Renda
Maria Concetta
MC
Hematology II-Thalassemia, Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy.
Giambona
Antonino
A
Fecarotta
Emanuela
E
Leto
Filippo
F
Makrydimas
George
G
Renda
Disma
D
Damiani
Gianfranca
G
Jakil
Maria Cristina
MC
Picciotto
Francesco
F
Piazza
Angela
A
Valtieri
Mauro
M
Maggio
Aurelio
A
D015703:Antigens, CD / Q000378:metabolism; D001826:Body Fluids / Q000166:cytology*; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017493:Leukocyte Common Antigens; D008534:Megaloblasts / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011990:Receptors, Transferrin / Q000378:metabolism; D015017:Yolk Sac / Q000502:physiology; D055547:epsilon-Globins / Q000235:genetics / Q000378:metabolism; D055545:gamma-Globins / Q000235:genetics / Q000378:metabolism
D015703:Antigens, CD; C068126:CD71 antigen; D011990:Receptors, Transferrin; D055547:epsilon-Globins; D055545:gamma-Globins; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human