Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry.
Comprehensive two-dimensional gas chromatography (GCxGC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GCxGC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958T by using parallel flame ionization detector (FID)/mass spectrometry (MS). The results are compared to data obtained using a thermal modulated GCxGC system. The data show that flow modulated GCxGC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria.
Gu
Qun
Q
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023 Dalian, China.
David
Frank
F
Lynen
Frédéric
F
Rumpel
Klaus
K
Xu
Guowang
G
De Vos
Paul
P
Sandra
Pat
P
D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis*; D005410:Flame Ionization; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D020615:Stenotrophomonas maltophilia / Q000737:chemistry*
D005227:Fatty Acids