Smoke exposure as a determinant of autoantibody titre in α₁-antitrypsin deficiency and COPD.
Liberation of elastin peptides from damaged lung may be a mechanism of autoimmune lung disease. Citrullination, and anti-citrullinated protein antibody formation occurs in smokers, but the role of smoking in autoantibody generation relevant to pulmonary disease is unclear. Anti-elastin, anti-cyclic citrullinated peptide (anti-CCP) and anti-mutated citrullinated vimentin (anti-MCV) antibodies were measured in 257 subjects with α₁-antitrypsin deficiency (AATD), 113 subjects with usual chronic obstructive pulmonary disease (COPD) and 22 healthy nonsmokers. Levels were compared between groups, against phenotypic features and against smoke exposure. Anti-elastin antibodies were higher in controls relative to AATD (p = 0.008) and usual COPD (p < 0.00001), and in AATD relative to usual COPD (p < 0.00001). Anti-elastin levels showed a threshold at 10 pack-yrs, being higher in those who had smoked less (p = 0.004). No relationships between antibody levels and clinical phenotype were seen after adjustment for smoke exposure. Anti-CCP antibodies were higher in usual COPD than AATD (p = 0.002) but the relationship to smoke exposure was less clear. Smoke exposure is the main determinant of anti-elastin antibody levels, which fall after 10 pack-yrs. Local antibody complexes may be a better measure of elastin directed autoimmunity than circulating levels.
College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK. a.m.wood@bham.ac.uk
A M
AM
Wood
P
P
de Pablo
C D
CD
Buckley
A
A
Ahmad
R A
RA
Stockley
D000328:Adult; D000368:Aged; D001327:Autoimmune Diseases / Q000276:immunology; D004549:Elastin / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008171:Lung Diseases / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology* / Q000276:immunology*; D012306:Risk; D012907:Smoking / Q000009:adverse effects*; D013997:Time Factors; D019896:alpha 1-Antitrypsin Deficiency / Q000276:immunology*
D004549:Elastin