Monoolein cubic phase containing acidic proteinoid: pH-dependent release.
OBJECTIVE
Monoolein (MO) cubic phase containing acidic proteinoid was prepared for a pH-dependent release.


METHODS
The acidic proteinoid was prepared by a thermal-condensation reaction of Asp and Leu (9.85/0.15 in molar ratio). To prepare MO cubic phase, molten MO was hydrated with the proteinoid solution in distilled water. For pH-dependent release experiment, amaranth was included as an anionic dye, and either auramine O or methylene blue was contained as a cationic dye.


RESULTS
The release of amaranth from the cubic phase was promoted under neutral and alkali conditions, possibly because of electrostatic repulsions between the anionic dye and the ionized carboxylic group of the acidic proteinoid. On the contrary, the releases of auramine O and methylene blue were suppressed under neutral and alkali conditions, probably because of electrostatic interactions between the cationic dyes and the ionized carboxylic group.


CONCLUSIONS
The acidic proteinoid is believed to control the releases in response to change in pH.
Kwon
Taek Kwan
TK
Division of Biotechnology & Bioengineering, Institute of Bioscience and Biotechnology, Kangwon National University, Chunchon, Korea.
Kim
Jin-Chul
JC
D000143:Acids / Q000737:chemistry; D000548:Amaranth Dye / Q000737:chemistry; D020346:Aquaporins / Q000737:chemistry; D001224:Aspartic Acid / Q000737:chemistry; D001576:Benzophenoneidum / Q000737:chemistry; D001682:Biological Availability; D004337:Drug Carriers / Q000138:chemical synthesis; D005989:Glycerides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D044367:Phase Transition; D011506:Proteins / Q000737:chemistry*
D000143:Acids; D020346:Aquaporins; D004337:Drug Carriers; D005989:Glycerides; D011506:Proteins; D001576:Benzophenoneidum; D001224:Aspartic Acid; D000548:Amaranth Dye; C005953:monoolein; D007930:Leucine; D008751:Methylene Blue