Release of satiety hormones in response to specific dietary proteins is different between human and murine small intestinal mucosa.
OBJECTIVE
High protein diets are the most effective to stimulate cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1) release; however, which proteins are the most potent is not known. Here, the effects of specific dietary proteins on intestinal CCK and GLP-1 release were examined.


METHODS
Duodenal biopsies of 10 healthy male subjects and 10 male rats were taken and placed in an Ussing chamber system. The biopsies were exposed on the luminal side to buffer, egg protein, codfish protein, ovomucoid, pea protein, and wheat protein. After an exposure time of 2 h, samples were taken from the serosal side.


RESULTS
Pea protein and wheat protein increased CCK and GLP-1 release in human duodenal tissue, while codfish protein only increased CCK release. No elevated levels of CCK and GLP-1 were found after exposure of rat tissue to different proteins.


CONCLUSIONS
Pea and wheat protein are the most potent stimulators of CCK and GLP-1 release in human duodenal tissue, and may therefore be good dietary additives in weight management.
Department of Human Biology, Maastricht University Medical Centre, Maastricht, The Netherlands. M.Geraedts @ HB.unimaas.nl
Maartje C P
MC
Geraedts
Freddy J
FJ
Troost
Rik
R
Tinnemans
Johan D
JD
Söderholm
Robert-Jan
RJ
Brummer
Wim H M
WH
Saris
D000328:Adult; D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D004044:Dietary Proteins / Q000494:pharmacology*; D015194:Diffusion Chambers, Culture; D004386:Duodenum / Q000187:drug effects / Q000378:metabolism; D004594:Electrophysiology; D048389:Gadus morhua; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010059:Ovomucin / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D012528:Satiety Response / Q000187:drug effects*
D004044:Dietary Proteins; D010940:Plant Proteins; D010059:Ovomucin; D052216:Glucagon-Like Peptide 1; D002766:Cholecystokinin