Electrooptical monitoring of cell polarizability and cell size in aerobic Escherichia coli batch cultivations.
The time-dependent development of cell polarizability and length in Escherichia coli batch fermentations were observed at-line with electrooptical measurements. While using a measurement system with fully automated sample preparation, the development of these properties can be observed with a comparable high frequency (six measurements per hour). The polarizability as well as the mean cell length both increase soon after inoculation and then decline from the growth phase on until the stationary phase is reached. Based on the dynamic behavior of polarizability, the growth phase can be divided into four distinct stages. Changes in the cultivation temperature or the pre-cultivation conditions lead to alterations in the development of the polarizability and mean cell length. Based on the frequency disperse of polarizability measured at four different frequencies from 210 to 2,100 kHz, a prediction model is established that is based on the relation of the polarizability to the metabolic activity. Applying multi-linear partial least squares methods (N-PLS), the model is able to predict the specific acetate synthesis and uptake with a root mean square error of prediction of 0.19 (6% of the mean). The method represents a tool for characterization of different stages with respect to microbial metabolic activity and the energy balance during batch cultivations.
Laboratory of Bioprocess Technology, Institute of Biotechnology, Technische Universität Berlin, Ackerstrasse 71-76, ACK 24, 13355 Berlin, Germany.
Stefan
S
Junne
M
M
Nicolas Cruz-Bournazou
Alexander
A
Angersbach
Peter
P
Götz
D000332:Aerobiosis; D019149:Bioreactors; D018929:Cell Culture Techniques; D016764:Cell Polarity; D055724:Electrophysiological Phenomena; D048168:Escherichia coli K12 / Q000166:cytology* / Q000254:growth & development*; D007218:Industrial Microbiology / Q000379:methods*; D008853:Microscopy; D013053:Spectrophotometry