Cholesterol orientation and tilt modulus in DMPC bilayers.
We performed molecular dynamics (MD) simulations of hydrated bilayers containing mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol at various ratios, to study the effect of cholesterol concentration on its orientation, and to characterize the link between cholesterol tilt and overall phospholipid membrane organization. The simulations show a substantial probability for cholesterol molecules to transiently orient perpendicular to the bilayer normal, and suggest that cholesterol tilt may be an important factor for inducing membrane ordering. In particular, we find that as cholesterol concentration increases (1-40% cholesterol) the average cholesterol orientation changes in a manner strongly (anti)correlated with the variation in membrane thickness. Furthermore, cholesterol orientation is found to be determined by the aligning force exerted by other cholesterol molecules. To quantify this aligning field, we analyzed cholesterol orientation using, to our knowledge, the first estimates of the cholesterol tilt modulus chi from MD simulations. Our calculations suggest that the aligning field that determines chi is indeed strongly linked to sterol composition. This empirical parameter (chi) should therefore become a useful quantitative measure to describe cholesterol interaction with other lipids in bilayers, particularly in various coarse-grained force fields.
Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York, USA. gek2009@med.cornell.edu
George
G
Khelashvili
Georg
G
Pabst
Daniel
D
Harries
D002784:Cholesterol / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure
D007202:Indicators and Reagents; D008051:Lipid Bilayers; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine