The effect of cell density on the maturation and contractile ability of muscle derived cells in a 3D tissue-engineered skeletal muscle model and determination of the cellular and mechanical stimuli required for the synthesis of a postural phenotype.
The successful engineering of a truly biomimetic model of skeletal muscle could have a significant impact on a number of biomedical disciplines. Although a variety of techniques are currently being developed, there is, as of yet, no widely available and easily reproducible culture system for the synthesis of 3D artificial muscle tissues. In attempting to generate such a model it is essential to optimise any protocol in order to generate a tissue that best represents the in vivo environment. Since the maturation of muscle derived cells in culture is critically dependent on density, a major factor to be addressed in the development of these models is the ideal concentration at which to seed cells in order to generate an optimal response. In studying the effect of cell density on the performance of cells in an established 3D collagen based model of skeletal muscle, we demonstrate that an optimum density does exist in terms of peak force generation and myogenic gene expression data. Greater densities however, lead to the formation of a more physiologically relevant tissue with a phenotype characteristic of slow, postural muscle.
UCL Institute of Orthopaedics and Musculoskeletal Science, London, UK.
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Mudera
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Smith
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Brady
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Lewis
D000328:Adult; D032701:Biomimetics; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D003094:Collagen / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D040542:Mechanotransduction, Cellular; D032342:Muscle Cells / Q000502:physiology*; D024510:Muscle Development / Q000235:genetics; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D055815:Young Adult
D012333:RNA, Messenger; D003094:Collagen