Osteoblast biocompatibility on poly(octanediol citrate)/sebacate elastomers with controlled wettability.
This work examines the biocompatibility of poly(octanediol citrate)/sebacate (p(OCS)) biodegradable polyester elastomers. The growth of human MG63 osteoblast-like cells was studied on p(OCS) films. Three types of p(OCS) films were synthesised simply by varying the concentrations of 1,8-octanediol (OD), citric acid (CA), and sebacic acid (SA) monomers at initial molar ratios of 1:1:0, 1:0.75:0.25 and 1:0.5:0.5. At these ratios, the p(OCS) films exhibit decreasing hydrophilicity as shown by the measured water contact angle values of 31, 41 and 64 degrees , respectively. For all the samples, no difference in cell growth was detected after 1 day of cell culture. However, after 4 days, the highest number of viable cells was detected on the p(OCS) film synthesised with the intermediate CA molar ratio of 0.75. This sample also contains the median concentration of surface carboxylic acid groups and hydrophilicity. Following long-term cell culture (18 days), a statistically significant higher density of viable cells had grown on the p(OCS) films with SA molar ratios of 0.25 (P < 0.0001) and 0.5 (P = 0.002) in comparison to the material containing 100% CA and no SA. The work demonstrated that the performance of possible p(OCS) bone tissue engineering scaffolds could be improved by simply adjusting the molar ratios of CA and SA in the pre-polymer without any requirements for post-synthesis modification.
Djordjevic
Ivan
I
Ian Wark Research Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, Adelaide, SA 5095, Australia.
Szili
Endre J
EJ
Choudhury
Namita Roy
NR
Dutta
Naba
N
Steele
David A
DA
Kumar
Sunil
S
D002264:Carboxylic Acids / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002951:Citrates / Q000737:chemistry* / Q000494:pharmacology*; D020724:Elastomers / Q000737:chemistry* / Q000494:pharmacology*; D005240:Feasibility Studies; D006801:Humans; D008422:Materials Testing; D019637:Orthopedic Procedures; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D017155:Wettability
D002264:Carboxylic Acids; D002951:Citrates; D020724:Elastomers; D011108:Polymers; C513817:poly(1,8-octanediol citrate)