Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes.
The diploid species Brassica rapa (genome AA) and B. oleracea (genome CC) were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China.
Fang
F
Kong
Cailin
C
Ge
Xiaoping
X
Fang
Rod J
RJ
Snowdon
Youping
Y
Wang
D001937:Brassica / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005819:Genetic Markers; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D036226:Seedlings / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity
D005819:Genetic Markers; D010940:Plant Proteins; D020543:Proteome