Gal d 6 is the second allergen characterized from egg yolk.
Only one allergen from the egg yolk, alpha-livetin (Gal d 5) has been described thus far. A new egg yolk allergen was detected studying 27 egg allergic patients. The study was performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting and IgE-immunoblotting-inhibition assays. An egg yolk extract was fractioned by reverse-phase high-performance liquid chromatography (RP-HPLC), and the new allergen detected was characterized by N-terminal amino acid analysis. A total of 5 of the 27 patients (18%) detected a yolk allergen of an apparent molecular weight of 35 kDa by SDS-PAGE. Heating and reduction treatments did not affect its allergenicity, although digestion with simulated gastric fluid diminished the IgE-binding capacity of the allergen. The N-terminal amino acid sequence corresponded with the YGP42 protein, a fragment of the vitellogenin-1 precursor. Thus, a second egg yolk allergen has been described and designated Gal d 6 by the World Health Organization (WHO)/International Union of Immunological Societies (IUIS) Allergen Nomenclature Subcommittee.
Department of Allergology, Complejo Asistencial, Avda. del Cid, Burgos, Spain.
Alvaro
A
Amo
Rosa
R
Rodríguez-Pérez
Juan
J
Blanco
Julian
J
Villota
Sonsoles
S
Juste
Ignacio
I
Moneo
María Luisa
ML
Caballero
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014819:Vitellogenins / Q000737:chemistry* / Q000276:immunology*; D055815:Young Adult
D000485:Allergens; D014819:Vitellogenins; D007073:Immunoglobulin E