4,6-dimethoxy-1,3,5-triazine oligoxyloglucans: novel one-step preparable substrates for studying action of endo-beta-1,4-glucanase III from Trichoderma reesei.
Two kinds of 4,6-dimethoxy-1,3,5-triazine (DMT) oligoxyloglucans, DMT-beta-XXXG and DMT-beta-XLLG, have been synthesized via one-step procedure starting from the corresponding unprotected oligoxyloglucans in water. The resulting DMT derivatives were found to be hydrolyzed by endo-beta-1,4-D-glucanase III from Trichoderma reesei (EGIII) and utilized as substrates for determination of the kinetic parameters of EGIII. The present DMT-method would be a convenient analytical tool for studying the action of glycosyl hydrolases due to the extremely simple synthetic process of DMT-glycosides without using protecting groups.
Graduate School of Engineering, Tohoku University, Aoba, Sendai, Japan.
Atsushi
A
Kobayashi
Tomonari
T
Tanaka
Kazuhito
K
Watanabe
Masaki
M
Ishihara
Masato
M
Noguchi
Hirofumi
H
Okada
Yasushi
Y
Morikawa
Shin-ichiro
S
Shoda
D044644:Endo-1,3(4)-beta-Glucanase / Q000378:metabolism*; D005936:Glucans / Q000138:chemical synthesis* / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity; D014227:Triazines / Q000138:chemical synthesis* / Q000494:pharmacology; D014242:Trichoderma / Q000201:enzymology*
D005936:Glucans; D014227:Triazines; D044644:Endo-1,3(4)-beta-Glucanase