Effects of fumonisin B1 on HLA class I antigen presentation and processing pathway in GES-1 cells in vitro.
Fumonisin B1 (FB1) is a food-borne mycotoxin produced by genus Fusarium and was classified as possible carcinogen to humans by the International Agency for Research on Cancer (IARC). Human leukocyte antigen (HLA) class I antigen presentation pathway plays an important role in immunosurveillance. Defects in HLA class I antigen presentation pathway can down-regulate the expression of HLA class I antigen on the surface of nucleated cells that will confer a survival advantage to randomly mutant cells and may lead to malignant transformation. In the present study, we analyzed the effects of FB1 on the expression of HLA class I heavy chain (classical HLA-A, -B and -C genes included), beta2-microglobulin (β2m), LMP2 and TAP1 genes in human gastric epithelial immortalized GES-1 cells in vitro using semi-quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR), Western blot and immunocytochemical methods in dose- and time-effect studies. Our results revealed that FB1 have an effect on HLA class I antigen presentation pathway via the decreased expression of HLA class I heavy chain and/or defects of LMP2 and TAP1 expression. However, the importance of this effect in carcinogenesis needs further investigation.
Laboratory of Pathology, Hebei Medical University, Shijiazhuang, Hebei, China.
Z G
ZG
Yao
X H
XH
Zhang
F
F
Hua
J
J
Wang
X
X
Xing
J L
JL
Wang
X
X
Yan
L X
LX
Xing
D017951:Antigen Presentation / Q000187:drug effects* / Q000235:genetics; D015153:Blotting, Western; D002274:Carcinogens, Environmental / Q000633:toxicity*; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology; D037341:Fumonisins / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D002274:Carcinogens, Environmental; D037341:Fumonisins; D015395:Histocompatibility Antigens Class I; C056933:fumonisin B1