A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing.
A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.
Umeda
Kaoru
K
Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka, Japan.
Seto
Yoshiyuki
Y
Kohda
Tomoko
T
Mukamoto
Masafumi
M
Kozaki
Shunji
S
D015373:Bacterial Typing Techniques / Q000379:methods; D019274:Botulinum Toxins, Type A / Q000235:genetics*; D001906:Botulism / Q000382:microbiology*; D047013:Clostridium botulinum type A / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods
D004269:DNA, Bacterial; D019274:Botulinum Toxins, Type A