[Study on fingerprints spectrum of Lophatherum gracile by HPLC].
OBJECTIVE
To establish the fingerprint spectrum of Lophatherum gracile by HPLC.


METHODS
The samples were separated on Agilent Eclipse XDB- C18 column (150 mm x 4.6 mm, 5 microm) with methanol (A) -3% acetic acid (B) as the mobile phase at a flow rate of 1.0 mL/min at 30 centigrades, adopting the gradient elution process with switching the Wavelength.


RESULTS
15 samples of different origin Lophatherum gracile Brongn were detected and 16 peaks in the chromatogram were common, there was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.


CONCLUSIONS
The method is accurate, reliable and the fingerprint spectrum provides more information for controlling the quality of Lophatherum gracile.
Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. 15840951126@139.com
Yan
Y
Guo
Yan-Hua
YH
Guo
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D006109:Poaceae / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents
D004365:Drugs, Chinese Herbal; D012997:Solvents