Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein.
Elements that contribute to the high, stable yield of soybean peroxidase (SBP) in soybean seed coats can be exploited in the development of this tissue as a protein production platform. SBP contains an N-terminal and a C-terminal propeptide that are predicted to direct vacuolar targeting; this may be one factor that contributes to its high yield and stability. We characterized the function of the SBP propeptides and investigated their ability to increase the yield of a foreign protein in a heterologous plant system. SBP propeptides are functional signal peptides capable of directing vacuolar transport in Arabidopsis. The use of these propeptides as well as an endoplasmic reticulum (ER)-retention signal to direct a foreign protein to the apoplast, ER, or vacuole can significantly increase yield and will therefore be useful for the development of the seed coat as a protein production platform. We also demonstrate that growth conditions may have a significant impact on the yield of a foreign protein and that this may be subcellular compartment-specific.
Schnell
Jaimie A
JA
Ottawa-Carleton Institute of Biology, University of Ottawa, PO Box 450, Station A, Ottawa, ON, K1N 6N5, Canada.
Han
Shuyou
S
Miki
Brian L
BL
Johnson
Douglas A
DA
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals; D013025:Glycine max / Q000201:enzymology*; D014617:Vacuoles / Q000378:metabolism
D011498:Protein Precursors; D021382:Protein Sorting Signals; D010544:Peroxidases