A strategy for the determination of the elemental composition by fourier transform ion cyclotron resonance mass spectrometry based on isotopic peak ratios.
We propose a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by high-magnetic field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Using 3'-phosphoadenosine 5'-phosphosulfate and CTU guanamine as standard organic compounds, isotopic peaks derived from (15)N-, (34)S-, and (18)O-substituted forms were separated from (13)C-substituted species. Furthermore, these isotopic peaks were quantitatively detected and closely matched the natural abundance of each element. These data successfully led us to determine the one elemental composition in a standard independent manner. The approach should be particularly amenable to the metabolomics research field.
Miura
Daisuke
D
Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 12-8582, Japan.
Tsuji
Yukiko
Y
Takahashi
Katsutoshi
K
Wariishi
Hiroyuki
H
Saito
Kazunori
K
D002247:Carbon Isotopes / Q000737:chemistry; D005583:Fourier Analysis; D007477:Ions / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods; D009587:Nitrogen Isotopes / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry*; D010103:Oxygen Isotopes / Q000737:chemistry; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry; D013460:Sulfur Isotopes / Q000737:chemistry
D002247:Carbon Isotopes; D007477:Ions; D009587:Nitrogen Isotopes; D009930:Organic Chemicals; D010103:Oxygen Isotopes; D013460:Sulfur Isotopes; D010724:Phosphoadenosine Phosphosulfate