Normal levels of p27 are necessary for somite segmentation and determining pronephric organ size.
The Xenopus laevis cyclin dependent kinase inhibitor p27(Xic1) has been shown to be involved in exit from the cell cycle and differentiation of cells into a quiescent state in the nervous system, muscle tissue, heart and retina. We show that p27(Xic1) is expressed in the developing kidney in the nephrostomal regions. Using overexpression and morpholino oligonucleotide (MO) knock-down approaches we show normal levels of p27(Xic1) regulate pronephros organ size by regulating cell cycle exit. Knock-down of p27(Xic1) expression using a MO prevented myogenesis, as previously reported; an effect that subsequently inhibits pronephrogenesis. Furthermore, we show that normal levels of p27(Xic1) are required for somite segmentation also through its cell cycle control function. Finally, we provide evidence to suggest correct paraxial mesoderm segmentation is not necessary for pronephric induction in the intermediate mesoderm. These results indicate novel developmental roles for p27(Xic1), and reveal its differentiation function is not universally utilised in all developing tissues.
Department of Biological Sciences; and Warwick University; Coventry, UK.
Richard W
RW
Naylor
Robert J
RJ
Collins
Anna
A
Philpott
Elizabeth A
EA
Jones