The transcriptional repression of platelet-derived growth factor receptor-beta by the zinc finger transcription factor ZNF24.
The zinc finger transcription factor ZNF24 is a relatively uncharacterized Krüppel-like transcription factor, displaying a suppressive effect on the transcription. ZNF24 is involved in negative regulation of vascular endothelial growth factor (VEGF) and may represent a novel repressor of VEGF transcription. In a previous study, our analysis reveals that ZNF24 is a pleiotropic factor that has a role in hematopoiesis, brain development and cancers. Activation of PDGFR-beta is critically involved into various cell functions including recruitment of stromal cells and VEGF induction in tumor and perivascular cells. Here, overexpression of ZNF24 resulted in a significant down-regulation of PDGFR-beta, whereas silencing of ZNF24 with small interfering RNA led to increased PDGFR-beta expression. Overexpression of ZNF24 resulted in a significant decrease in PDGFR-beta promoter activity. These data suggest that ZNF24 is involved in negative regulation of PDGFR-beta and may represent a novel repressor of PDGFR-beta transcription.
Department of Biochemical Pharmacy, Second Military Medical University, Shanghai, China. lijianzhong1234@yahoo.com
Jianzhong
J
Li
Xia
X
Chen
Ying
Y
Liu
Li
L
Ding
Lei
L
Qiu
Zhenlin
Z
Hu
Junping
J
Zhang
D002460:Cell Line; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation; D016335:Zinc Fingers
D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C457300:ZNF24 protein, human; D020797:Receptor, Platelet-Derived Growth Factor beta