Suppression of embryonic lung branching morphogenesis by antisense oligonucleotides against HOM/C homeobox factors.
The role of HOM/C homeobox genes on rat embryonic lung branching morphogenesis was investigated using the lung bud explant culture system in an air/liquid interface. Knock down of homeobox b3 and b4 expression by antisense oligonucleotide treatment repressed airway branch formation, while antisense oligonucleotide against homeobox a3 showed no effect. Addition of antisense Hoxb3 oligonucleotide resulted in upregulation of collagen type III mRNA and fibroblast growth factor 10 mRNA, while that of the T-box regulatory factor-4 was decreased. Consequently, expression of Clara cell-specific secretory protein was decreased. These results suggest a critical role for homeobox b3 and b4 genes in lung airway branching morphogenesis.
Laboratory of Environmental Molecular Physiology, School of Life Sciences, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan. vzz05106@nifty.com
Tatsuya
T
Yoshimi
Fumiko
F
Hashimoto
Shigeru
S
Takahashi
Yuji
Y
Takahashi
D000818:Animals; D024061:Collagen Type III / Q000235:genetics; D051526:Fibroblast Growth Factor 10 / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D009024:Morphogenesis; D016376:Oligonucleotides, Antisense / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D024061:Collagen Type III; C496465:Fgf10 protein, rat; D051526:Fibroblast Growth Factor 10; D018398:Homeodomain Proteins; D016376:Oligonucleotides, Antisense