Enhancement of gastric ulcer healing and angiogenesis by cochinchina Momordica seed extract in rats.
Cochinchina momordica seed is the dried ripe seed of Momordica cochinchinensis, a perennial vine. The antiulcer effect of an extract from cochinchina momordica seeds (SK-MS10) was evaluated in a rat model of acetic acid-induced gastric ulcers. Gastric ulcers were produced by subserosal injection of acetic acid. SK-MS10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. The stomach was removed and the ulcer size measured at day 7 and 14 of the treatment. Expression of vascular endothelial growth factor (VEGF) was assessed by real-time RT-PCR and Western blot analysis. In addition, the microvasculature density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry. The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats. The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant. These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.
Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Jung Mook
JM
Kang
Nayoung
N
Kim
Bongcheol
B
Kim
Joo-Hyon
JH
Kim
Bong-Yong
BY
Lee
Ji Hyun
JH
Park
Mi Kyoung
MK
Lee
Hye Seung
HS
Lee
Joo Sung
JS
Kim
Hyun Chae
HC
Jung
In Sung
IS
Song
D019342:Acetic Acid / Q000633:toxicity; D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D005169:Factor VIII / Q000378:metabolism; D008297:Male; D029775:Momordica / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D000897:Anti-Ulcer Agents; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; D005169:Factor VIII; D019342:Acetic Acid