Selective down-regulation of Th2 cytokines by C-terminal binding protein 2 in human T cells.
BACKGROUND
Among several C-terminal binding proteins (CtBPs), friend of GATA (FOG) has been implicated in the down-regulation of GATA-3-mediated Th2 cell differentiation. Here we investigated the role of CtBP2 in Th1 and Th2 cytokine expression in human T cells.


METHODS
CtBP2 was introduced into human peripheral CD4+ T cells by a lentiviral transduction system. Subsequently, the expression of Th1 and Th2 cytokine mRNA was determined by quantitative real-time RT-PCR.


RESULTS
CtBP2 significantly suppressed stimulation-induced expression of IL-4, IL-5 and IL-13 in human T cells. However, IFN-gamma expression was not affected by the introduction of CtBP2.


CONCLUSIONS
CtBP2 selectively down-regulates Th2 cytokines, therefore it is a potential target for the treatment of allergic diseases.
Department of Allergy and Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
Fujiko
F
Kitamura
Noriko
N
Kitamura
Akio
A
Mori
Hideki
H
Tatsumi
Soichi
S
Nemoto
Hiroyuki
H
Miyoshi
Shoichiro
S
Miyatake
Takachika
T
Hiroi
Osamu
O
Kaminuma
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D056970:Co-Repressor Proteins; D016207:Cytokines / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D018793:Interleukin-13 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D015848:Interleukin-5 / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D016086:Lentivirus / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018417:Th1 Cells / Q000187:drug effects / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000378:metabolism*; D014161:Transduction, Genetic
D056970:Co-Repressor Proteins; D016207:Cytokines; C508598:IL4 protein, human; C508599:IL5 protein, human; D018793:Interleukin-13; D015848:Interleukin-5; D009419:Nerve Tissue Proteins; D015847:Interleukin-4; D015759:Ionomycin; D007371:Interferon-gamma; D000429:Alcohol Oxidoreductases; C421248:CTBP2 protein, human; D013755:Tetradecanoylphorbol Acetate