The hepatic genes for immunoproteasome are upregulated by refeeding after fasting in the rat.
Considering that animals maintain energy homeostasis in response to nutrient levels, experiments were done to elucidate the temporal effects of refeeding after fasting on gene expression profiles in the rat liver. Using DNA microarray technology, we first compared gene expression profiles in the livers of rats allowed to feed for 6 h after fasting for 18 h and those in 24-h fasting rats, and found that the expression levels of energy metabolism-related genes in the two groups were different. In addition, refeeding induced upregulation of the genes encoding immunoproteasome components. Finally, immunoblot analysis confirmed changes in protein levels, suggesting that refeeding after fasting enhanced immune function.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Shota
S
Ushiama
Toshiaki
T
Nakamura
Tomoko
T
Ishijima
Takumi
T
Misaka
Keiko
K
Abe
Yuji
Y
Nakai
D000821:Animal Feed; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D005215:Fasting; D020869:Gene Expression Profiling; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D008297:Male; D046988:Proteasome Endopeptidase Complex / Q000235:genetics* / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000276:immunology*
D046988:Proteasome Endopeptidase Complex