Hesperidin promotes cyclobutane pyrimidine dimer repair in UVB-exposed mice epidermis.
OBJECTIVE
To investigate whether topical application of hesperin affords protection to Balb/C mice epidermis from UVB-induced cyclobutane pyrimidine dimers (CPDs).


METHODS
A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining and southwestern dot blotting were used for CPDs detection; western blotting was used for P53 detection.


RESULTS
Topical application of hesperidin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 24 and 48 h after 180 mJ/cm(2) of UVB irradiation as compared to untreated mice. UVB-induced p53 expression was more pronounced in hesperidin-treated mice epidermis compared to that of untreated mice.


CONCLUSIONS
Taken together, these results suggest that topical hesperidin application promotes DNA photo-damage repair. Hesperidin is therefore a promising protective substance against UVB radiation.
Department of Dermatology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
S
S
Jin
B
B
Zhou
D
D
Luo
D000287:Administration, Topical; D000818:Animals; D026501:Blotting, Southwestern; D015153:Blotting, Western; D004249:DNA Damage / Q000187:drug effects*; D045643:DNA Repair Enzymes / Q000502:physiology; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006569:Hesperidin / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000502:physiology; D011740:Pyrimidine Dimers / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D011740:Pyrimidine Dimers; D016159:Tumor Suppressor Protein p53; D006569:Hesperidin; D045643:DNA Repair Enzymes