Efficiency of acid phosphatases secreted from the ectomycorrhizal fungus Hebeloma cylindrosporum to hydrolyse organic phosphorus in podzols.
Ectomycorrhizal fungi may improve the phosphate nutrition of their host plants by secreting, into the soil solution, acid phosphatases (AcPases) able to release orthophosphate (Pi) from soil organic phosphorus (Po). Using cation-exchange chromatography, we separated four fractions with AcPase activity secreted by the ectomycorrhizal fungus Hebeloma cylindrosporum grown in a pure culture under P-starved conditions. Each AcPase active fraction displayed strong ability in vitro to hydrolyse a wide range of phosphate monoesters, but none of them efficiently hydrolysed phytate. Their efficiency to release Pi from soil NaHCO(3)-extractable Po was studied in a sandy podzol used intact or autoclaved. Soils were collected in a 15-year-old Pinus pinaster stand, receiving regular fertilization or not. Autoclaving increased the NaHCO(3)-extractable Po concentrations by 55% in unfertilized and by 32-43% in fertilized soils. The efficiency of each AcPase fraction was affected significantly by the soil fertilization regime and the soil treatment (intact vs. autoclaved). The proportion of labile Po enzyme ranged from 0% to 11% and 14% to 48% after 1 h of incubation in bicarbonate solutions extracted from intact and autoclaved soils, respectively. This work suggests that AcPases secreted from H. cylindrosporum could be efficient in recycling Po pools from soil microorganisms that may be delivered by soil autoclaving.
UMR 1222 Ecologie Fonctionnelle et Biogéochimie des Sols (INRA-IRD-SupAgro, UMR 210), Montpellier, France.
Julien
J
Louche
Muhammad Arif
MA
Ali
Benoît
B
Cloutier-Hurteau
François-Xavier
FX
Sauvage
Hervé
H
Quiquampoix
Claude
C
Plassard
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D001639:Bicarbonates / Q000737:chemistry; D055364:Hebeloma / Q000201:enzymology*; D006868:Hydrolysis; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D028223:Pinus / Q000382:microbiology; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013379:Substrate Specificity
D001639:Bicarbonates; D012987:Soil; D010758:Phosphorus; D000135:Acid Phosphatase