[To identify a novel HLA-DRB1 allele in Chinese by sequencing].
OBJECTIVE
To identify a novel HLA-DRB1 allele in Chinese.


METHODS
A novel HLA-DR allele was detected by PCR-SSP and SBT in a patient with leukemia.


RESULTS
The sequence of the novel allele was different from all other known alleles. The novel allele differed from the closet matching allele HLA-DRB1*1404 by one nucleotide substitution in exon 2, at position 33 T>C, this resulted in an amino acid change from Tyr to His at codon 17.


CONCLUSIONS
The novel allele is confirmed as a new HLA allele and it was officially named HLA-DRB1*1461 by WHO Nomenclature Committee in May, 2006.
HLA Laboratory, Beijing Red Cross Blood Center, Beijing, 100088 PR China.
Wei
W
Li
Xiao-yan
XY
Shan
Na
N
Liu
Lei
L
Ni
Li-jun
LJ
Wang
Lin
L
Wang
Shuang
S
Cui
Xiao-mei
XM
He
Zhi-yin
ZY
Gong
Bo-tao
BT
Zhao
Zhi-xin
ZX
Zhang
D000483:Alleles; D044466:Asian People / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains