POT1 deficiency alters telomere length and telomere-associated gene expression in human gastric cancer cells.
Telomeres are the end structures of linear chromosomes in eukaryotic cells. The integrity of a telomere is essential for the overall stability of the chromosome. The human protection of telomeres 1 (hPOT1) protein, a single-stranded telomeric DNA binding protein, plays an important role in telomere protection and telomere length regulation. Here, we show that the loss of hPOT1 by RNA interference in BGC823 (poorly differentiated human gastric adenocarcinoma) cells leads to an increase in multinucleated giant cells, a decrease in cell proliferation and colony formation, induction of senescence and apoptosis, shortened telomere length, upregulation of the TRF1 gene and downregulation of the TRF2, tankyrase1 and hTERT genes. These results suggest that the loss of hPOT1 results in a decrease in the viability of BGC823 cells; hPOT1 regulates telomere length positively and has an influence on the expression of other telomere-associated genes in the cells.
Institute of Gastroenteropathy, Southwest Hospital, Third Military Medical University, Chongqing, China.
Xiaoyan
X
Ning
Shiming
S
Yang
Rongquan
R
Wang
Rugang
R
Zhang
Liping
L
Guo
Jun
J
Tie
Yongbo
Y
Cheng
Guoan
G
Wang
Shunmei
S
Wan
Dianchun
D
Fang
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D002470:Cell Survival; D015870:Gene Expression; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D000089804:Shelterin Complex; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D038501:Tankyrases / Q000235:genetics; D019098:Telomerase / Q000235:genetics; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure*; D034501:Telomere-Binding Proteins / Q000172:deficiency*; D035321:Telomeric Repeat Binding Protein 1 / Q000235:genetics; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics; D014407:Tumor Cells, Cultured
D034741:RNA, Small Interfering; D000089804:Shelterin Complex; D038501:Tankyrases; D019098:Telomerase; D034501:Telomere-Binding Proteins; D035321:Telomeric Repeat Binding Protein 1; D035341:Telomeric Repeat Binding Protein 2; C440849:POT1 protein, human; C418109:TNKS protein, human