Immunomodulatory effects of IFN-beta and lovastatin on immunophenotype of monocyte-derived dendritic cells in multiple sclerosis.
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system and current MS treatment is only partially effective. Recent data suggest that statins may be potent immunomodulatory agents. In order to evaluate their role in MS, we analyzed the in vitro effects of interferon (IFN)-beta and lovastatin on the differentiation and maturation of monocyte-derived dendritic cells (DCs) of MS patients. Twenty-seven patients with relapsing-remitting MS were recruited for the study. DC differentiation and maturation were evaluated based on surface phenotypic changes and the expressions of CD14, CD83, CD1a, CD80, CD86, CD206, and C209 were analyzed by flow cytometry. The results showed that IFN-beta and lovastatin affect DC phenotype. Both agents decrease the expression of CD1a, which indicates a weakened presentation of glycolipid antigens. IFN-beta causes up-regulated and lovastatin down-regulated expression of CD86, which results in a biased Th-cell responses in MS. Furthermore, high doses of lovastatin cause a decrease in CD209 expression on the surface of DCs and can limit their migration to various tissues. One of the mechanisms of the beneficial action of IFN-beta and statins may be associated with their influence on DCs.
Department of Neurology, Medical University of Lublin, Jaczewskiego 8, 20-090 Lublin, Poland. bartosikpsujek@op.pl
Halina
H
Bartosik-Psujek
Jacek
J
Tabarkiewicz
Krystyna
K
Pocinska
Sebastian
S
Radej
Zbigniew
Z
Stelmasiak
Jacek
J
Rolinski
D000328:Adult; D000924:Anticholesteremic Agents / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D015703:Antigens, CD / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D016130:Immunophenotyping; D016899:Interferon-beta / Q000276:immunology / Q000627:therapeutic use; D008148:Lovastatin / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D055815:Young Adult
D000924:Anticholesteremic Agents; D015703:Antigens, CD; D007155:Immunologic Factors; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta; D008148:Lovastatin