Characterizing proteins of unknown function: orphan cytochrome p450 enzymes as a paradigm.
With the rapid completion of genomic sequences of organisms today, we have far more gene products than functions we can ascribe. A number of experimental strategies have been developed and applied, both in vitro and in vivo, to put functions to these orphan proteins. The "deorphanization" of human and Streptomyces cytochrome P450 enzymes is considered quite important for pharmacology, with ramifications for the use of clinical therapeutics. The myriad of possibilities is too enormous to screen one reaction at a time, thus metabolomic or proteomic screens with complex biological samples are promising current strategies.
Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232-0146, USA. f.guengerich@vanderbilt.edu
F Peter
FP
Guengerich
Zhongmei
Z
Tang
S Giovanna
SG
Salamanca-Pinzón
Qian
Q
Cheng
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D023281:Genomics; D006801:Humans; D055432:Metabolomics; D040901:Proteomics; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System