Reduction of miR-21 induces glioma cell apoptosis via activating caspase 9 and 3.
Extensive data indicate that miR-21 plays a critical role in gliomagenesis, however, knowledge is limited on the mechanism of action of miR-21, including cell proliferation, apoptosis, and migration. In this study, we showed that down-regulation of miR-21 expression by antisense oligonucleotides inhibited glioma cell proliferation and induced cell apoptosis. Moreover, reduction of miR-21 activated caspase 9 and 3, which may be mediated by modulating multiple potential target genes, such as TIMP3. Together, these findings indicate that miR-21 plays a key role in regulating cell apoptosis in gliomas and may serve as a target for effective therapies.
Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, PR China.
Xuan
X
Zhou
Junxia
J
Zhang
Qiang
Q
Jia
Yu
Y
Ren
Yingyi
Y
Wang
Lei
L
Shi
Ning
N
Liu
Guangxiu
G
Wang
Peiyu
P
Pu
Yongping
Y
You
Chunsheng
C
Kang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology* / Q000627:therapeutic use; D023041:Xenograft Model Antitumor Assays
C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D020319:Oligodeoxyribonucleotides, Antisense; D053148:Caspase 3; D053453:Caspase 9