14-3-3 mediates apelin-13-induced enhancement of adhesion of monocytes to human umbilical vein endothelial cells.
To investigate whether apelin-13 induced THP-1 monocytes (MCs) adhesion to ECV304 human umbilical vein endothelial cells (HUVECs) via 14-3-3 signaling transduction pathway and the potential novel physiological function and signaling transduction pathway of apelin-APJ, HUVECs ECV304 were cultured in DMEM and MCs THP-1 were cultured in RPMI 1640 medium. Monocyte adhesion and the expression of vascular cell adhesion molecule-1 (VCAM-1) and 14-3-3 were measured with monocyte adhesion assay and western blot analysis. Data showed that apelin-13 increased adhesion of MCs to HUVECs in a concentration- and time-dependent manner, which reached their peaks at 1 mM and 12 h, respectively. Similarly, apelin-13 induced the expression of HUVECs adhesion molecule, VCAM-1, in a concentration- and time-dependent manner, reached their peaks at 1 microM and 12 h, respectively. Apelin-13 induced the expression of 14-3-3 in a concentration- and timedependent manner, which reached their peaks at 1 mM and 5 min, respectively. Furthermore, the potent 14-3-3 inhibitor difopein significantly reduced the expression of 14-3-3 and VCAM-1 in apelin-13 stimulated HUVECs, and difopein significantly inhibited the effect of apelin-13 on induction of MCs adhesion to HUVECs. These data suggested that 14-3-3 mediated the induction of adhesion of MCs to HUVECs by Apelin-13.
Institute of Pharmacy and Pharmacology, University of South China, Hengyang, China.
Xin
X
Li
Xianhui
X
Zhang
Fang
F
Li
Linxi
L
Chen
Lanfang
L
Li
Xuping
X
Qin
Jing
J
Gao
Tao
T
Su
Yixin
Y
Zeng
Duanfang
D
Liao
D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D009000:Monocytes / Q000502:physiology*; D011247:Pregnancy; D011506:Proteins / Q000494:pharmacology; D015398:Signal Transduction; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000378:metabolism
D048948:14-3-3 Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D019010:Vascular Cell Adhesion Molecule-1; C495199:apelin-13 peptide; C443131:difopein