Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division.
The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) regulates cellular motility and the synthesis of organelles and molecules that promote adhesion to a variety of biological and nonbiological surfaces. These properties likely require tight spatial and temporal regulation of c-di-GMP concentration. We have developed genetically encoded fluorescence resonance energy transfer (FRET)-based biosensors to monitor c-di-GMP concentrations within single bacterial cells by microscopy. Fluctuations of c-di-GMP were visualized in diverse Gram-negative bacterial species and observed to be cell cycle dependent. Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa. Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function.
Christen
Matthias
M
Department of Immunology, University of Washington, Seattle, WA 98195, USA.
Kulasekara
Hemantha D
HD
Christen
Beat
B
Kulasekara
Bridget R
BR
Hoffman
Lucas R
LR
Miller
Samuel I
SI
D015374:Biosensing Techniques; D016935:Caulobacter crescentus / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D029968:Escherichia coli Proteins; D031541:Fluorescence Resonance Energy Transfer; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000378:metabolism; D008853:Microscopy; D009068:Movement; D009154:Mutation; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000166:cytology / Q000378:metabolism; D015290:Second Messenger Systems
D029968:Escherichia coli Proteins; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP