A practical strategy for the characterization of coumarins in Radix Glehniae by liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry.
The aim of the present study was to develop a practical method for the characterization of coumarins in Radix Glehniae by liquid chromatography-mass spectrometry (LC-MS). First, 10 coumarin standards (including two pairs of isomers) were studied, and mass spectrometry fragmentation patterns and elution time rules for the coumarins were found. Then, an extract of Radix Glehniae was analyzed by the combination of two scan modes, i.e., multiple ion monitoring-information-dependent acquisition-enhanced product ion mode (MIM-IDA-EPI) and precursor scan information-dependent acquisition-enhanced product ion mode (PREC-IDA-EPI) on a hybrid triple quadrupole-linear ion trap mass spectrometer. A total of 41 coumarins were identified on the basis of their mass spectrometry fragmentation patterns. This is the first time that these two scan modes have been combined to characterize chemical constituents in traditional Chinese medicine. This new method allowed the identification of coumarins in Radix Glehniae in trace amounts. The methodology proposed in this study could be valuable for the structural characterization of coumarins from complex natural and synthetic sources.
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang 050017, China.
Wei
W
Yang
Min
M
Ye
Man
M
Liu
Dezhi
D
Kong
Rui
R
Shi
Xiaowei
X
Shi
Kerong
K
Zhang
Qiao
Q
Wang
Zhang
Z
Lantong
D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003374:Coumarins / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry
D003374:Coumarins; D004365:Drugs, Chinese Herbal