[Experimental study of immune reactivity in Swiss mice due to topical administration of three textile dyes].
Reactive Red 183, Reactive Red 2 and Reactive Blue 204 (red dye, green dye and blue dye) are three reactive dyes frequently used in textile industry. In some atmospheric conditions ( high temperature, perspiration, pH values, UV/IR radiations), some quantities of these hydrolyzed dyes, could pass from textile clothes directly into the human skin.


METHODS
There were used 4 groups of white Swiss mice (with similar weight and number of both sexes), control group and 3 groups, treated once daily with a retro-auricular application of different reactive dyes. After 14 days of treatment, blood samples were taken from retro-orbitary plexus to assess leukocyte count, phagocytic capacity of peripheral neutrophils, serum opsonic capacity, phagocyte capacity and bactericidal capacity of peritoneal macrophages, splenic T lymphocytes with rossetting capacity and spleen cells forming Jerne plaques. The retro-acuricular and latero-cervical nodes were weighted.


RESULTS
Red dye did not influence the weight of the studied nodes, but determined statistically significant modifications on non-specific immune system parameters. Blue and grena dyes determined modifications of weight especially of retroauricular nodes. Grena dye determined important effects of non-specific immune system parameters (serum opsonic capacity, phagocyte capacity and bactericidal capacity of peritoneal macrophages). The blue dye did not determine a biological response.


CONCLUSIONS
Red and green dye determined important effects on non-specific immune system parameters.
Gavăt
C C
CC
Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi Facultatea de Bioinginerie Medicală, Disciplina de Tehnologie protetică şi Biotehnologii Facultatea de Medicinfă.
Lupuşoru
R V
RV
Ghiciuc
Cristina Mihaela
CM
Lupuşoru
Cătălina Elena
CE
D000279:Administration, Cutaneous; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004396:Coloring Agents / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007155:Immunologic Factors / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology; D012867:Skin / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013783:Textile Industry; D013784:Textiles
D015415:Biomarkers; D004396:Coloring Agents; D007155:Immunologic Factors; D009895:Opsonin Proteins