Serial oxygen equilibrium and kinetic measurements during RBC storage.
OBJECTIVE
To contribute to the understanding of the biochemical changes associated with the RBC storage lesion.


OBJECTIVE
To investigate changes in O(2) equilibrium and on/off kinetic rates during routine cold storage.


BACKGROUND
As RBCs are stored between 1 and 6°C numerous biochemical changes occur within the RBCs, including changes in the properties of the haemoglobin itself. This study serially analysed for the first time the O(2) equilibrium and on/off kinetic rates across the RBC membrane during routine storage.


METHODS
The oxygen binding (k(on) ) and offloading (k(off) ) constants were measured in fresh RBCs and then in AS-5-preserved RBCs at weekly intervals, along with oxygen equilibrium curves (OECs), 2,3-Diphosphoglycerate (2,3-DPG), p50 and the Hill number (n).


RESULTS
The k(on) increased slightly as the 2,3-DPG and p50 decreased during storage, whereas the k(off) remained largely unchanged. The OECs demonstrated the expected increase in O(2) affinity, whereas the Hill number was unchanged during storage.


CONCLUSIONS
In spite of the biochemical, structural and functional changes associated with the storage of RBCs, their in vitro interactions with oxygen were largely preserved through 42 days of storage.
Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland, USA.
M P
MP
Gelderman
M H
MH
Yazer
Y
Y
Jia
F
F
Wood
A I
AI
Alayash
J G
JG
Vostal
D019794:2,3-Diphosphoglycerate / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods / Q000592:standards*; D017707:Erythrocyte Transfusion / Q000592:standards; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate; D010100:Oxygen