Switching off FRET by analyte-induced decomposition of squaraine energy acceptor: A concept to transform 'turn off' chemodosimeter into ratiometric sensors.
FRET cassette-type sensors, SN-1 and SN-2, which contain two naphthalimide donors and a squaraine acceptor, have been developed on the concept of switching off FRET through removing the spectral overlap by the analyte-induced decompositions of acceptor chromophore. SN-2 shows sensitive responses to fluoride anion and cyanide through both naked-eye detectable colour changes and ratiometric fluorescence changes. As the first example, SN-2 confirms that transformation of the common 'turn-off' chemodosimeter into ratiometric sensors can be realized conveniently based on the novel 'switching FRET off' approach.
Stake Key Laboratory of Fine Chemicals, Dalian University of Technology, Zhongshan Road 158, Dalian 116012, China.
Haibo
H
Yu
Meiyan
M
Fu
Yi
Y
Xiao
D000838:Anions; D003486:Cyanides / Q000032:analysis / Q000737:chemistry; D003503:Cyclobutanes / Q000737:chemistry*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D005459:Fluorides / Q000032:analysis / Q000737:chemistry; D008956:Models, Chemical; D053644:Naphthalimides / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*
D000838:Anions; D003486:Cyanides; D003503:Cyclobutanes; D005456:Fluorescent Dyes; D053644:Naphthalimides; D010636:Phenols; C480596:squaraine; D005459:Fluorides