Antioxidant and radiation antagonistic effect of Saraca indica.
The present study was undertaken to investigate the radiation mitigating effect of hydro-alcoholic extract of Saraca indica (SIE) against mice exposed to whole body gamma radiation. Free radical scavenging ability by SIE was demonstrated using hydroxyl, superoxide anion, ABTS*+ and DPPH radicals generated in vitro. A significant increase in the animal survival (dose reduction factor = 1.39), inhibition in the decline of hematological parameters as well as increased number of spleen colony-forming units was observed when SIE was administered prior to radiation. SIE pretreatment increased the levels of glutathione, glutathione S- trans-ferase, catalase and lowered lipid peroxidation. Our findings for the first time demonstrate the protective potential of SIE against radiation induced syndromes with an optimal dose of 400 mg/kg b.wt. The radiation mitigating effect may be attributed to the mechanisms such as free radical scavenging, elevation in antioxidant status, and reduction in lipid peroxidation.
Rao
Bola Sadashiva Satish
BS
Department of Radiobiology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India. satishraomlsc@gmail.com
Rao
Nageshwar
N
Archana
P R
PR
Gayathri
P
P
Shetty
Prerana
P
D000818:Animals; D001773:Blood Cells / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D029911:Caesalpinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D004353:Drug Evaluation, Preclinical; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008136:Longevity / Q000187:drug effects; D008297:Male; D051379:Mice; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects; D023382:Toxicity Tests, Acute; D014916:Whole-Body Irradiation / Q000009:adverse effects
D016166:Free Radical Scavengers; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D010088:Oxidoreductases; D005978:Glutathione