Association analysis of retinoic acid receptor beta (RARbeta) gene with high myopia in Chinese subjects.
OBJECTIVE
High myopia or pathological myopia is a common refractive error. Individuals with high myopia are subject to increased risk of serious eye complications. Accumulating evidence has demonstrated the role for heritability in ocular growth and in the development of high myopia. Retinoic acid and retinoic acid receptors play important roles in ocular development and in experimentally induced myopia. The purpose of this study was to determine if high myopia is associated with single nucleotide polymorphism (SNP) variants in the retinoic acid receptor beta (RARbeta) gene in Chinese subjects.


METHODS
DNA samples were purified from venous lymphocytes of 175 unrelated Chinese patients with high myopia (less than -8.00 diopters) and 101 Chinese control subjects without high myopia (+/-1.00 diopters). Direct nucleotide sequence analysis in the RARbeta gene was performed, and the detected variations were further confirmed by reverse sequencing. Allelic frequencies of all detected SNPs were assessed for Hardy-Weinberg equilibrium.


RESULTS
Five variations in RARbeta were detected in Chinese subjects with high myopia, including 32574G>A, 32629G>A, 32645C>T, 32647T>G, and 151973C>T, of which only 32647T>G (NCBI notes as rs58244688 and rs2067964) had already been reported. The majority of SNP genotypes were heterozygous. While 32647T>G, 32629G>A, and 32645C>T were located in introns and 32574G>A and 151973C>T were located in coding regions, none of the SNPs affected the amino acid sequence. In the present study, no evidence of association was found between variations in the nucleotide sequence of RARbeta and high myopia.


CONCLUSIONS
Five SNP variants in RARbeta were detected in Chinese subjects with high myopia, none of them were associated significantly with high myopia. Further studies are needed to identify which genes are responsible for high myopia.
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou, Zhejiang, P. R. China.
Yang
Y
Ding
Xiaoyan
X
Chen
Dongsheng
D
Yan
Anquan
A
Xue
Fan
F
Lu
Jia
J
Qu
Xiangtian
X
Zhou
D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002648:Child; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007438:Introns; D008875:Middle Aged; D009216:Myopia / Q000503:physiopathology*; D016366:Open Reading Frames; D020641:Polymorphism, Single Nucleotide; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D012720:Severity of Illness Index; D055815:Young Adult
D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta