[Association of MICA gene polymorphism and serum soluble MICA level with colorectal cancer].
OBJECTIVE
To investigate whether the major histocompatibility complex class I chain-related gene A gene (MICA) polymorphism and serum soluble MICA level were associated with the occurrence and development of colorectal cancer.


METHODS
DNA samples from 117 colorectal cancer patients and 113 healthy individuals from Yangzhou in Jiangsu province were genotyped by using the polymerase chain reaction (PCR) and sequence-specific primer (SSP) method and PCR based sequencing. In addition, polymorphism at position 129 was also analyzed by PCR-SSP. Serum levels of soluble MICA were measured by a sandwich ELISA method.


RESULTS
Neither the extracellular nor the transmembrane region polymorphisms of MICA gene were associated with the occurrence and the different stages of colorectal cancer. In contrast, the frequency of the methionine residue at position 129 was significantly decreased in the patient group. Soluble MICA levels in sera were increased in the late stages of colorectal cancer.


CONCLUSIONS
Although there was no genetic susceptibility attributed to MICA gene polymorphism with regard to development of colorectal cancer, serum levels of soluble MICA may be a diagnostic marker of advanced stages.
Department of Immunology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu, 225001 PR China. wjgong@yzu.edu.cn
Wei-juan
WJ
Gong
Wei-ming
WM
Xiao
Chun-xiang
CX
Gong
Fang
F
Tian
Ming-chun
MC
Ji
D015179:Colorectal Neoplasms / Q000097:blood* / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000097:blood* / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A