Identification of a major yolk protein as an allergen in sea urchin roe.
Anaphylaxis after eating sea urchin roe has been reported. However, its major allergens have not yet been identified. The aim of this study was to identify the major allergens of sea urchin roe. Proteins of sea urchin roe were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis (2-DE). An immunoglobulin (Ig)E-binding protein was detected by immunoblotting using the patient's serum. An allergen isolated from 2DE-gel was identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Immunoblot analysis of sea urchin extracts showed that a 160-kDa protein at pI 6-7 was recognized by the patient's IgE. Peptide mass fingerprint analysis revealed that the protein was the major yolk protein (152 kDa, pI 6.9) of sea urchins. The results show that a major allergen of sea urchin roe is the major yolk protein.
Yamasaki
Ayako
A
Division of Dermatology, Department of Medicine of Sensory and Motor Organs, Faculty of Medicine, Tottori University, Yonago, Japan. ayama813@grape.med.tottori-u.ac.jp
Higaki
Hiromi
H
Nakashima
Keiko
K
Yamamoto
Osamu
O
Hein
Kyaw Zaw
KZ
Takahashi
Hitoshi
H
Chinuki
Yuko
Y
Morita
Eishin
E
D000328:Adult; D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use; D000906:Antibodies / Q000097:blood; D004527:Egg Proteins / Q000009:adverse effects* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005512:Food Hypersensitivity / Q000188:drug therapy / Q000209:etiology* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007428:Intradermal Tests; D008970:Molecular Weight; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods; D012617:Sea Urchins / Q000276:immunology; D017747:Seafood / Q000009:adverse effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016896:Treatment Outcome
D018926:Anti-Allergic Agents; D000906:Antibodies; D004527:Egg Proteins