[Effect of bushen antai recipe and its various compositions on endometrial sexual hormones and receptors in mice with blastocyst implantation dysfunction].
OBJECTIVE
To compare the effects and mechanisms of Bushen Antai Recipe (BAR) and its different compositions, Bushen Recipe (BSR, the Shen-nourishing part) and Huoxue Recipe (HXR, the blood-activating part) on the endometrial receptivity in mice with blastocyst implantation dysfunction (BID).


METHODS
Model mice of BID induced by indomethacin were treated respectively with BAR, BSR and HXR from the first day of pregnancy, and killed on day 5 or 6. Samples of their serum and uterine were collected for detecting serum estradiol (E2), progesterone (P) contents using radioimmunoassay; and endometrial expression levels of their receptors, ER and PR, using immunohistochemistry.


RESULTS
Serum levels of E2 and P, and endometrial expression of ER and PR increased significantly on day 6 in mice after treated by BAR, but in those treated by BSR and HXR, the improvements were significantly lesser.


CONCLUSIONS
BAR, by combined application of both Shen-nourishing and blood-activating methods, could impact E2, P, ER and PR to improve the endometrial receptivity to a higher extent in BID mice so as to promote embryo implantation.
Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030. mmzhang@tjh.tj-mu.edu.cn
Ming-min
MM
Zhang
Xin-fang
XF
He
Jin-jin
JJ
Zhang
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000097:blood; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C534019:bushen antai; D011374:Progesterone; D004958:Estradiol