Antiinflammatory and antinociceptive activities of Zingiber zerumbet methanol extract in experimental model systems.
OBJECTIVE
The present study was carried out to determine the antiinflammatory and antinociceptive activities of a methanol extract of Zingiber zerumbet rhizomes (MEZZ) using various experimental model systems.


METHODS
The MEZZ was prepared by macerating oven-dried (50 degrees C) powdered rhizomes (1.2 kg) of Z. zerumbet in 80% methanol in a ratio of 1:20 (w/v) for 48 h. The supernatant was collected, filtered and evaporated to dryness under reduced pressure (50 degrees C) yielding approximately 21.0 g of the crude dried extract. The crude dried extract was stored at -20 degrees C prior to use and was dissolved in normal saline (0.9% NaCl) immediately before administration at concentrations required to produce doses of 25, 50 and 100 mg/kg.


RESULTS
All dosages of MEZZ showed significant (p < 0.05) antiedema activity when assessed using the carrageenan-induced paw edema test and the cotton-pellet-induced granuloma test. The MEZZ exhibited significant (p < 0.05) antinociceptive activity when assessed by the writhing, hot plate and formalin tests. Pretreatment with naloxone (5 mg/kg) significantly decreased the latency of discomfort produced by the 100 mg/kg dose of MEZZ in the hot plate test.


CONCLUSIONS
MEZZ produced antiinflammatory and antinociceptive activities which may involve the inhibition of bradykinin-, prostaglandin-, histamine- and opioid-mediated processes.
Zakaria
Z A
ZA
Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Mohamad
A S
AS
Chear
C T
CT
Wong
Y Y
YY
Israf
D A
DA
Sulaiman
M R
MR
D000700:Analgesics / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001920:Bradykinin; D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D020939:Zingiber officinale / Q000737:chemistry*; D006632:Histamine; D008297:Male; D000432:Methanol / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011453:Prostaglandins; D051381:Rats; D017207:Rats, Sprague-Dawley
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D011453:Prostaglandins; D009270:Naloxone; D006632:Histamine; D002351:Carrageenan; D001920:Bradykinin; D000432:Methanol