Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS.
An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2-12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.
Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China.
Honggang
H
Nie
Ranran
R
Liu
Youyou
Y
Yang
Yu
Y
Bai
Yafeng
Y
Guan
Daqing
D
Qian
Tao
T
Wang
Huwei
H
Liu
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D057230:Limit of Detection; D008055:Lipids / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010537:Peritoneum / Q000033:anatomy & histology / Q000737:chemistry; D025341:Principal Component Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers; D008055:Lipids