ESR detection of ROS generated by TiO2 coated with fluoridated apatite.
Specific materials used in the manufacture of dentures may enhance the removal of micro-organisms. The ultraviolet A (UVA) irradiation of acrylic resin containing titanium dioxide (TiO(2)) generates reactive oxygen species (ROS) by photocatalysis that shows antibacterial effects. In this study, we tested the hypothesis that TiO(2) coated with fluoridated apatite (FAp-TiO(2)) can generate ROS via photo-catalysis by using electron spin resonance (ESR), and that acrylic resin containing FAp-TiO(2) can show antifungal properties by measuring the viability of Candida albicans. We demonstrated that hydroxyl radicals (HO(*)) were generated through excitation of TiO(2), TiO(2) coated with apatite (HAp-TiO(2)), and FAp-TiO(2). The HO(*) generation through excitation of FAp-TiO(2) was higher than that of TiO(2) and HAp-TiO(2). Regarding antifungal activity, cell viability on acrylic resin containing FAp-TiO(2) was lower than that of TiO(2) and HAp-TiO(2). FAp-TiO(2) showed superior photocatalytic effects, and these characteristics may lead to novel methods for the clinical application of denture-cleaning treatments.
Division of Removal Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College, Yokosuka, Kanagawa, Japan.
Tomofumi
T
Sawada
F
F
Yoshino
K
K
Kimoto
Y
Y
Takahashi
T
T
Shibata
N
N
Hamada
Tomoji
T
Sawada
M
M
Toyoda
M-C
MC
Lee
D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology* / Q000528:radiation effects; D000935:Antifungal Agents / Q000494:pharmacology* / Q000528:radiation effects; D001031:Apatites / Q000494:pharmacology* / Q000528:radiation effects; D002176:Candida albicans / Q000187:drug effects*; D003819:Denture Bases / Q000382:microbiology*; D003820:Denture Cleansers; D004338:Drug Combinations; D004578:Electron Spin Resonance Spectroscopy; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D019097:Spin Trapping / Q000379:methods*; D014025:Titanium / Q000494:pharmacology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000180:Acrylic Resins; D000935:Antifungal Agents; D001031:Apatites; D003820:Denture Cleansers; D004338:Drug Combinations; C009495:titanium dioxide; D017665:Hydroxyl Radical; D014025:Titanium; C025105:fluorapatite