Metabolomic analysis of thermally injured and/or septic rats.
BACKGROUND
Early diagnosis and treatment for thermal injury with septic complications continue to be a serious clinical problem. In this study, plasma biomarkers of rats in the burn and/or septic models were investigated with a metabolomic method.


METHODS
Rat plasma samples were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Multivariate analysis, the principal components analysis (PCA), was used to validate metabolic changes. In addition, another multivariate method, the orthogonal partial least-squares analysis (OPLS), was used to profile potential biomarkers in models.


RESULTS
Nine characteristic metabolites, including hypoxanthine, indoxyl sufate, glucuronic acid, gluconic acid, proline, uracil, nitrotyrosine, uric acid, and trihydroxy cholanoic acid were identified in models of thermal injury and/or sepsis.


CONCLUSIONS
These biomarkers were mainly involved in oxidative stress and tissue damage, and might supply evidence for distinguishing burned septic patients from non-septic ones.
Department of Medicinal Chemistry of Nature Product, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Xin-Ru
XR
Liu
Xing-Feng
XF
Zheng
Shi-Zhao
SZ
Ji
Yong-Hai
YH
Lv
De-Yi
DY
Zheng
Zhao-Fan
ZF
Xia
Wei-Dong
WD
Zhang
D000596:Amino Acids / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002056:Burns / Q000097:blood*; D002264:Carboxylic Acids / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004195:Disease Models, Animal; D006571:Heterocyclic Compounds / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis
D000596:Amino Acids; D015415:Biomarkers; D002264:Carboxylic Acids; D006571:Heterocyclic Compounds