Quantitative assessment of somatosensory-evoked potentials after cardiac arrest in rats: prognostication of functional outcomes.
OBJECTIVE
High incidence of poor neurologic sequelae after resuscitation from cardiac arrest underscores the need for objective electrophysiological markers for assessment and prognosis. This study aims to develop a novel marker based on somatosensory evoked potentials (SSEPs). Normal SSEPs involve thalamocortical circuits suggested to play a role in arousal. Due to the vulnerability of these circuits to hypoxic-ischemic insults, we hypothesize that quantitative SSEP markers may indicate future neurologic status.


METHODS
Laboratory investigation.


METHODS
University Medical School and Animal Research Facility.


METHODS
: Sixteen adult male Wistar rats.


METHODS
None.


RESULTS
SSEPs were recorded during baseline, during the first 4 hrs, and at 24, 48, and 72 hrs postasphyxia from animals subjected to asphyxia-induced cardiac arrest for 7 or 9 mins (n = 8/group). Functional evaluation was performed using the Neurologic Deficit Score (NDS). For quantitative analysis, the phase space representation of the SSEPs-a plot of the signal vs. its slope-was used to compute the phase space area bounded by the waveforms recorded after injury and recovery. Phase space areas during the first 85-190 mins postasphyxia were significantly different between rats with good (72 hr NDS >or=50) and poor (72 hr NDS <50) outcomes (p = .02). Phase space area not only had a high outcome prediction accuracy (80-93%, p < .05) during 85-190 mins postasphyxia but also offered 78% sensitivity to good outcomes without compromising specificity (83-100%). A very early peak of SSEPs that precedes the primary somatosensory response was found to have a modest correlation with the 72 hr NDS subscores for thalamic and brainstem function (p = .066) and not with sensory-motor function (p = .30).


CONCLUSIONS
Phase space area, a quantitative measure of the entire SSEP morphology, was shown to robustly track neurologic recovery after cardiac arrest. SSEPs are among the most reliable predictors of poor outcome after cardiac arrest; however, phase space area values early after resuscitation can enhance the ability to prognosticate not only poor but also good long-term neurologic outcomes.
Department of Biomedical Engineering, John Hopkins University School of Medicine, Baltimore, MD, USA.
Jai
J
Madhok
Anil
A
Maybhate
Wei
W
Xiong
Matthew A
MA
Koenig
Romergryko G
RG
Geocadin
Xiaofeng
X
Jia
Nitish V
NV
Thakor
D000704:Analysis of Variance; D000818:Animals; D001237:Asphyxia / Q000150:complications; D001784:Blood Gas Analysis; D004195:Disease Models, Animal; D005069:Evaluation Studies as Topic; D005073:Evoked Potentials, Somatosensory; D006323:Heart Arrest / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D009460:Neurologic Examination; D011379:Prognosis; D012372:ROC Curve; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018570:Risk Assessment; D018709:Statistics, Nonparametric; D015996:Survival Rate