[Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA].
Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFalpha) are over-expressed in a variety of tumors. Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here, a chimeric protein of EGF and TGFalpha (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA), a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies, which recognize both EGF and TGFalpha. Anti- E5T-mSEA serum at dilution of 1:10 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.
Department of Life Science, Anhui University, Hefei 230039, China.
Qingqing
Q
Yin
Haiwei
H
Jia
Yanhong
Y
Zhang
Chuanxuan
C
Liu
Qingjun
Q
Ma
Buchang
B
Zhang
Hui
H
Zhong
Quanbin
Q
Xu
D000595:Amino Acid Sequence; D000818:Animals; D019496:Cancer Vaccines / Q000096:biosynthesis* / Q000276:immunology; D045744:Cell Line, Tumor; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis* / Q000235:genetics
D019496:Cancer Vaccines; D004768:Enterotoxins; D011993:Recombinant Fusion Proteins; D016211:Transforming Growth Factor alpha; C026066:enterotoxin A, Staphylococcal; D004815:Epidermal Growth Factor; D066246:ErbB Receptors