[Effect of different storage methods and time on seed germination of Bletilla striata].
OBJECTIVE
Exploring seed storage method to offer a technological support for the seedling development of Bletilla striata.


METHODS
Bletilla striata seeds were stored by means of sand storage, nature storage and low temperature storage for 120, 150 and 180 d respectively. The germination rates were measured with methods of seed germination on the Petri dish and difference of rates was compared by means of the SSR method.


RESULTS
The embryo rate of Bletilla striata seeds was (84.9 +/- 4.7)%; the germination rate of fresh seeds was (92.1 +/- 2.1)%; the germination rates of seeds treated by sand storage, nature storage and low temperature storage for 120, 150 and 180 d were (89.3 +/- 2.7)%, (77.4 +/- 3.3)%, (68.9 +/- 2.7)% respectively and they showed significant difference or extreme significant difference one another. When seeds stored 120, 150 and 180 d, germination rates of seeds treated with three methods were (81.7 +/- 8.0)%, (78.5 +/- 7.9)%, (75.7 +/- 8.5)% respectively, and in these three storage times, germination rates of seeds stored 120 d and 180 d showed significant difference. In addition, there was no significant difference between germination rates of fresh seeds and sand storage seeds stored 120 d.


CONCLUSIONS
The effect of germination of seeds stored with sand was good, and germination rate was high steal after 180 d.
The Research Institute of Medicine Plantation of Chongqing, Chongqing 408435, China. hkz316@tom.com
Kai-Zhi
KZ
Hu
Jie
J
Liu
Bo
B
Xiao
Yan-Qin
YQ
Liu
Jing
J
Qin
Ru-Gang
RG
Han
D000383:Agriculture / Q000379:methods*; D018525:Germination; D029595:Orchidaceae / Q000254:growth & development*; D035281:Plant Tubers / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development; D013696:Temperature; D013997:Time Factors