In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold.
In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, P. R. China. imwangyj@scut.edu.cn
Yingjun
Y
Wang
Xuetao
X
Shi
Li
L
Ren
Yongchang
Y
Yao
Dong-An
DA
Wang
D019386:Alendronate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003692:Delayed-Action Preparations; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D006016:Glycolates / Q000737:chemistry; D019344:Lactic Acid; D055595:Mechanical Phenomena; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013583:Synovial Membrane / Q000166:cytology*; D054457:Tissue Scaffolds
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006016:Glycolates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003907:Dexamethasone; D017886:Durapatite; D019386:Alendronate