A point mutation in a glutamate-gated chloride channel confers abamectin resistance in the two-spotted spider mite, Tetranychus urticae Koch.
The molecular mechanisms and genetics of abamectin resistance mediated by target site insensitivity in the two-spotted spider mite, Tetranychus urticae, were investigated by comparing two isogenic abamectin-susceptible (AbaS) and abamectin-resistant (AbaR) strains. Cloning and sequencing of full-length cDNA fragments of gamma-amino butyric acid (GABA)-gated chloride channel genes revealed no polymorphisms between the two strains. However, sequence comparison of the full-length cDNA fragment of a T. urticae glutamate-gated chloride channel gene (TuGluCl) identified a G323D point mutation as being tentatively related with abamectin resistance. In individual F(2) progenies obtained by backcrossing, the G323D genotype was confirmed to correlate with abamectin resistance. Bioassays using progeny from reciprocal crossings revealed that the abamectin resistance trait resulting from TuGluCl insensitivity is incompletely recessive.
Kwon
D H
DH
Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
Yoon
K S
KS
Clark
J M
JM
Lee
S H
SH
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D018118:Chloride Channels / Q000737:chemistry / Q000235:genetics*; D003433:Crosses, Genetic; D004950:Esterases / Q000378:metabolism; D005260:Female; D005838:Genotype; D040582:Inheritance Patterns / Q000187:drug effects / Q000235:genetics; D007305:Insecticide Resistance / Q000187:drug effects* / Q000235:genetics*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D017354:Point Mutation / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D040102:Tetranychidae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D018118:Chloride Channels; C109777:glutamate-gated chloride channels; C048324:abamectin; D007559:Ivermectin; D006899:Mixed Function Oxygenases; D004950:Esterases