Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli.
Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme beta-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-beta-D-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.
Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA.
Agnese
A
Jurkevica
H Brian
HB
Halsall
Carl J
CJ
Seliskar
William R
WR
Heineman
D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development*; D007118:Immunoassay / Q000379:methods*; D050296:Microbial Viability