Direct and indirect effects of kisspeptin on liver oxidant and antioxidant systems in young male rats.
Kisspeptin is a recently discovered hypothalamic peptide which plays an important role in the central control of reproductive functions. We have investigated direct and indirect effects of kisspeptin on the liver oxidative stress in young male rats. Twenty-four rats were divided into four groups (n = 6/group). First group served as control and received saline. Kisspeptin-10 was administered to the animals in the second group (20 nmol/rat/day), for a period of 7 days. Rats were given only one dose gosereline (0.9 mg/rat), a GnRH agonist in the third group. The last group received kisspeptin-10 with gosereline. The activities of catalase, superoxide dismutase (SOD), xanthine oxidase (XO), adenosine deaminase (AD) and level of malondialdehyde were studied in liver tissue. Serum samples were separated for total antioxidant capacity (TAC), total oxidant status (TOS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, blood urea nitrogen (BUN), colesterol, high-density lipoprotein (HDL) and triglyceride. Kisspeptin increased the activities of SOD and catalase (p < 0.05). When compared to the control group, the levels of malondialdehyde, TOS and AST were lower, but levels of BUN, cholesterole, HDL and AD were higher in the other three groups (p < 0.05). In conclusion, our findings suggest that kisspeptin may have antioxidant and thus protective effects on the liver tissue.
Department of Physiology, Mustafa Kemal University, Hatay, Turkey. dr.maydin@yahoo.com <dr.maydin@yahoo.com>
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M
Aydin
S
S
Oktar
Z
Z
Yonden
O H
OH
Ozturk
B
B
Yilmaz
D000243:Adenosine Deaminase / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D017273:Goserelin / Q000494:pharmacology; D059648:Kisspeptins; D008075:Lipoproteins, HDL / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014969:Xanthine Oxidase / Q000378:metabolism
D018931:Antineoplastic Agents, Hormonal; C106628:KISS1 protein, human; D059648:Kisspeptins; D008075:Lipoproteins, HDL; D009842:Oligopeptides; D017382:Reactive Oxygen Species; D014280:Triglycerides; D017273:Goserelin; D008315:Malondialdehyde; D002784:Cholesterol; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D000243:Adenosine Deaminase