Treatment of surfaces with poly-L-lysine for Drosophila cell cultivation.
Most commonly used Drosophila cell lines adhere only loosely to the substrate. For some purposes (e.g., time-lapse photography and cytological examination of living cells), it is desirable to fix the growing cells more firmly to the substrate. As described in this protocol, this can be accomplished by treating the substrate with poly-L-lysine, with no obvious detrimental effect on the growth of the cells.
Lucy
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Cherbas
Peter
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Cherbas
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000166:cytology*; D011107:Polylysine / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects
D011107:Polylysine