Effects of montmorillonite on alleviating dietary Cd-induced oxidative damage in carp (Carassius auratus).
The present study was designed to investigate the effects of montmorillonite (MMT) on dietary Cd-induced oxidative damage in liver and kidney of carp (Carassius auratus). One hundred eighty carp were randomly divided into four groups and fed with a basal diet, a basal diet supplemented with 0.5% MMT, Cd-comtaminated basal diet (120 mg Cd/kg dry weight) and Cd-contaminated basal diet supplemented with 0.5% MMT, respectively. After 60 days, fish were sacrificed to measure malondialdehyde (MDA) content and antioxidative indices in liver and kidney. The results showed that the exposure of carp to dietary Cd caused decreases in glutathione peroxidase activity, catalase activity, superoxide dismutase activity, glutathione content and total antioxidant capacity level, while MMT supplemented in diet compensated Cd-induced decreases in above antioxidant indices to some extent in liver and kidney. As compared with the control group, increases in MDA content were observed in both measured tissues of carp exposed to dietary Cd, while MDA content decreased in carp exposed to Cd-contaminated basal diet supplemented with MMT in comparison with the Cd-contaminated group. It was suggested that MMT, when co-administered with Cd in diet, could alleviate dietary Cd-induced oxidative damage in liver and kidney of carp.
Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou, 310029, People's Republic of China.
Song Gwan
SG
Kim
Wei
W
Dai
Zirong
Z
Xu
Guanghuan
G
Li
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001546:Bentonite / Q000627:therapeutic use*; D002104:Cadmium / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000662:veterinary*; D004032:Diet / Q000662:veterinary; D005393:Fish Diseases / Q000139:chemically induced* / Q000188:drug therapy; D006054:Goldfish; D007674:Kidney Diseases / Q000188:drug therapy / Q000662:veterinary*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity
D000975:Antioxidants; D014874:Water Pollutants, Chemical; D002104:Cadmium; D001546:Bentonite; D008315:Malondialdehyde