Application of 2-DE for studying the variation of blood proteome.
2D electrophoresis (2-DE) is still a widely used proteomic method despite the availability of high-throughput approaches for protein identification. However, a literature survey only revealed a relatively small number of 2-DE-based studies of human blood proteome. We critically reviewed comparative 2-DE-based proteomic studies, in which groups of patients under examination involved more than ten individuals. Limitation on the number of samples is explained by the requirement to take into account the individual variation of blood proteome. The scanty amount of statistical data on quantitative variations of normal blood proteome may be one of the reasons for the poor applicability of proteomic biomarkers in clinical diagnostics.
State Scientific Center of the Russian Federation Institute for Biomedical Problems of the Russian Academy of Sciences, Khoroshevskoye shosse 76A, Moscow, Russian Federation. oxana.trifonova@gmail.com
Oxana
O
Trifonova
Irina
I
Larina
Anatoly
A
Grigoriev
Andrey
A
Lisitsa
Sergei
S
Moshkovskii
Alexander
A
Archakov
D001798:Blood Proteins / Q000378:metabolism*; D004194:Disease; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006262:Health; D006801:Humans; D020543:Proteome / Q000378:metabolism*
D001798:Blood Proteins; D020543:Proteome