Strategies for quantitation of phosphoproteomic data.
Recent developments in phosphoproteomic sample-preparation techniques and sensitive mass spectrometry instrumentation have led to large-scale identifications of phosphoproteins and phosphorylation sites from highly complex samples. This has facilitated the implementation of different quantitation strategies in order to study the biological role of protein phosphorylation during disease progression, differentiation or during external stimulation of a cellular system. In this article, a brief summary of the most popular strategies for phosphoproteomic studies is given; however, the main focus will be on different quantitation strategies. Methods for metabolic labeling, chemical modification and label-free quantitation and their applicability or inapplicability in phosphoproteomic studies are discussed.
Palmisano
Giuseppe
G
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Thingholm
Tine E
TE
D000595:Amino Acid Sequence; D006801:Humans; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D010766:Phosphorylation; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software
D010750:Phosphoproteins