The incidence of ANA and ETI-dsDNA detected by enzyme immunoassays and indirect immunofluorescence assay (IFA).
While the SLE (Systemic Lupus Erythematosus) specificity of ANA is low, that of anti-dsDNA autoantibodies is high. The DNA used in the assay must be double stranded: autoantibodies to single-stranded (ss) DNA exist in many diseases and specific to none. The prevalence (70%) of anti-dsDNA autoantibodies is much higherin SLE, giving a higher diagnostic sensitivity than the similarly disease-specific anti-Sm autoantibodies (30%). Anti-dsDNA autoantibodies are usually detected by very analytically sensitive techniques, such as ELISA (Enzyme Linked Immunosorbent Assay). Within SLE, ds-DNA autoantibodies tend to associate with the presence of glomerulonephritis. Their levels are used to monitor disease activity. We suggest the use of ds-DNA to find the difference between SLE patientswith benign variants and classical syndrome of severe skin and renal disease.
Aganovic-Musinovic
Izeta
I
Center for Genetics, Faculty of Medicine, University of Sarajevo, Cekalusa 80, 71000 Sarajevo, Bosnia and Herzegovina.
Prljaca-Zecevic
Lamija
L
Subasic
Djemo
D
D000974:Antibodies, Antinuclear / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D015415:Biomarkers; D004247:DNA