Surfactant protein-C promoter variants associated with neonatal respiratory distress syndrome reduce transcription.
Dominant mutations in coding regions of the surfactant protein-C gene, SFTPC, cause respiratory distress syndrome (RDS) in infants. However, the contribution of variants in noncoding regions of SFTPC to pulmonary phenotypes is unknown. By using a case-control group of infants > or =34 weeks gestation (n = 538), we used complete resequencing of SFTPC and its promoter, genotyping, and logistic regression to identify 80 single nucleotide polymorphisms (SNPs). Three promoter SNPs were statistically associated with neonatal RDS among European descent infants. To assess the transcriptional effects of these three promoter SNPs, we selectively mutated the SFTPC promoter and performed transient transfection using MLE-15 cells and a firefly luciferase reporter vector. Each promoter SNP decreased SFTPC transcription. The combination of two variants in high linkage dysequilibrium also decreased SFTPC transcription. In silico evaluation of transcription factor binding demonstrated that the rare allele at g.-1167 disrupts a SOX (SRY-related high mobility group box) consensus motif and introduces a GATA-1 site, at g.-2385 removes a MZF-1 (myeloid zinc finger) binding site, and at g.-1647 removes a potential methylation site. This combined statistical, in vitro, and in silico approach suggests that reduced SFTPC transcription contributes to the genetic risk for neonatal RDS in developmentally susceptible infants.
Wambach
Jennifer A
JA
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, 660 South Euclid, St. Louis, MO 63110, USA. wambach_j@kids.wustl.edu
Yang
Ping
P
Wegner
Daniel J
DJ
An
Ping
P
Hackett
Brian P
BP
Cole
F S
FS
Hamvas
Aaron
A
D000818:Animals; D001483:Base Sequence; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D015810:Linkage Disequilibrium; D016015:Logistic Models; D008156:Luciferases; D051379:Mice; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D037721:Pulmonary Surfactant-Associated Protein C / Q000235:genetics* / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics*; D020506:Untranslated Regions / Q000235:genetics; D044465:White People / Q000235:genetics
D037721:Pulmonary Surfactant-Associated Protein C; C489679:SFTPC protein, human; D020506:Untranslated Regions; D008156:Luciferases