Advanced oxidation protein products and total antioxidant activity in colorectal carcinoma.
The present study was designed to assess the levels of advanced oxidation protein products (AOPP) and percent hemolysis (that indirectly indicates the degree of membrane damage secondary to lipid peroxidation) in colorectal carcinoma. Glutathione (GSH), total thiols and albumin were measured to determine the antioxidant status. Considering the dynamic interaction between various antioxidants in the body, we measured the total antioxidant activity (AOA). Globulin was measured to assess the inflammatory response secondary to oxidative stress. Investigations were conducted in 45 cases of recently diagnosed primary colorectal adenocarcinoma. As control, 45 age and sex matched healthy persons were chosen. GSH was estimated in whole blood, percent hemolysis in RBC suspension and other parameters in plasma. We observed a very high significant increase (P<0.001) in AOPP, percent hemolysis and a highly significant increase (P<0.01) in globulin in colorectal carcinoma. We observed a very high significant decrease (P<0.001) in whole blood GSH, total thiols, albumin, AOA and a significant decrease (P<0.05) in plasma GSH in colorectal carcinoma. A very high significant negative correlation between percent hemolysis and AOA and an apparent negative correlation between total thiols and AOPP was seen in colorectal carcinoma. This demonstrated oxidative stress, decreased antioxidant status and secondary inflammatory response in colorectal carcinoma.
Department of Biochemistry, Centre for Basic Sciences, Kasturba Medical College, Bejai, Mangalore - 575 004.
S S
SS
Avinash
M
M
Anitha
Vinodchandran
Gayathri M
GM
Rao
K
K
Sudha
Beena V
BV
Shetty
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000097:blood*; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D005916:Globulins / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D012709:Serum Albumin / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000097:blood
D000975:Antioxidants; D001798:Blood Proteins; D005916:Globulins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D005978:Glutathione