Expression of divergent LSU rRNA genes in the Vibrio vulnificus CMCP6 genome during both infection and non-pathogenic stages.
The Vibrio vulnificus CMCP6 genome harbors nine copies of divergent large subunit (LSU) rRNA genes that may express and constitute four kinds of LSU rRNA molecules in a single cell. Primer extension analyses showed that these heterogeneous LSU rRNA transcripts are all expressed and assembled into ribosomes during both infection and nonpathogenic stages. Phylogenetic analyses of the internal transcribed spacer between SSU and LSU genes indicated that rRNA operons of V. vulnificus CMCP6 can be clustered into three distinct groups in rRNA genes of closely related Vibrio species. These findings imply that divergent rRNA genes in V. vulnificus CMCP6 resulted from interspecies recombination events in V. species, while the consequences of expression of heterogeneous rRNA molecules are not clear.
Kim
Hyun-Lee
HL
Department of Biomedical Science, College of Life Science, CHA University, Seongnam, 463-836, Republic of Korea.
Ryou
Sang-Mi
SM
Lee
Minho
M
Lee
Jin-Won
JW
Lee
Kangseok
K
Bae
Jeehyeon
J
D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012338:RNA, Ribosomal, 23S / Q000096:biosynthesis* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D041261:Vibrio vulnificus / Q000235:genetics* / Q000254:growth & development*
D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S