[Association of alpha 2-Heremans-Schmid glycoprotein gene polymorphisms with its serum levels].
OBJECTIVE
To identify the single nucleotide polymorphisms (SNPs) of the alpha2-Heremans-Schmid glycoprotein (AHSG) gene and assess their association with the AHSG serum level.


METHODS
The SNPs of the AHSG gene were identified from 30 unrelated Han individuals from Guangzhou area by resequencing. Linkage disequilibrium(LD) was performed to observe the linkage disequilibrium pattern. Then tagSNPs were genotyped in 192 Han individuals from Beijing and 424 Han individuals from Guangzhou area. Finally, luciferase reporter gene assay was performed to determine whether the SNPs affected the promoter activity. Serum AHSG concentrations were measured in the 192 subjects from Beijing using ELISA.


RESULTS
Eight SNPs were detected in total. The linkage disequilibrium profile in the Guangzhou Han population was different from that in the Beijing Han population. However, the allele and genotype frequencies of tagSNPs between the two Han populations were not significantly different. The reporter gene assay showed that the -799A allele had significantly higher promoter activity than the -799T allele. Multiple regression analysis revealed that only the rs2248690 SNP was an independent contributor to serum AHAG concentration.


CONCLUSIONS
The rs2248690 SNP in the promoter region of the AHSG gene might affect the AHSG gene transcription.
The Center of Clinical Genetics, Affiliated Bayi Children's Hospital, General Hospital of Beijing Command of the People's Liberation Army, Beijing, 100700 PR China.
Yan
Y
Wang
Yan
Y
Hou
Chun-zhi
CZ
Wang
Xiao-hui
XH
Zhu
Li-na
LN
Zhu
Xiao
X
Yang
Cheng
C
Cao
D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044967:Serum / Q000737:chemistry*; D060749:alpha-2-HS-Glycoprotein
D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein