Effect of NGF on the motility and acrosome reaction of golden hamster spermatozoa in vitro.
Motility and fertilizing ability are known to be two important physiological attributes of a mature sperm, yet the mechanism by which spermatozoa mature and become motile remains largely unknown. It has been shown that nerve growth factor (NGF) is a protein essential for the development, maintenance and survival of the peripheral and central nervous systems. However, the presence of high levels of NGF protein and mRNA do not correlate with the innervations by NGF sensitive fibers in tissues such as the testis, prostate and seminal vesicles. These observations have shifted the attention of research to the role of NGF outside of the nervous system. Here, we demonstrate that NGF and its receptors TrkA and p75 are widely expressed in the testis, accessory reproductive organ, and the epididymal sperms. We also show that NGF stimulates two important aspects of sperm functions, motility and the acrosome reaction, in a time- and dose-dependent manner. NGF activated the sperm cell acrosome reaction, while addition of inhibitors specific for MAPK kinase significantly blocked the sperm acrosome reaction. Taken together, our findings suggest that NGF plays an integral role in sperm motility and the acrosome reaction through, at least in part, the MAPK signalling pathway.
Jin
WanZhu
W
Laboratory of Veterinary Physiology, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Tanaka
Akane
A
Watanabe
Gen
G
Matsuda
Hiroshi
H
Taya
Kazuyoshi
K
D020101:Acrosome Reaction / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D008297:Male; D008647:Mesocricetus; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism / Q000502:physiology; D020917:Receptor, trkA / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014018:Tissue Distribution
D020800:Receptor, Nerve Growth Factor; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020929:Mitogen-Activated Protein Kinase Kinases