Quantitation of cholesterol incorporation into extruded lipid bilayers.
Cholesterol incorporation into lipid bilayers, in the form of multilamellar vesicles or extruded large unilamellar vesicles, has been quantitated. To this aim, the cholesterol contents of bilayers prepared from phospholipid:cholesterol mixtures 33-75 mol% cholesterol have been measured and compared with the original mixture before lipid hydration. There is a great diversity of cases, but under most conditions the actual cholesterol proportion present in the extruded bilayers is much lower than predicted. A quantitative analysis of the vesicles is thus required before any experimental study is undertaken.
Unidad de Biofísica (Centro Mixto CSIS-UPV/EHU), and Departamento de Bioquímica, Universidad del País Vasco, Apto. 644, 48080 Bilbao, Spain.
Maitane
M
Ibarguren
Alicia
A
Alonso
Boris G
BG
Tenchov
Felix M
FM
Goñi
D002784:Cholesterol / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D012995:Solubility; D013109:Sphingomyelins / Q000737:chemistry
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; C483858:phosphatidylethanolamine; D002784:Cholesterol