Acetaldehyde plasma polymer-coated PET fibers for endothelial cell patterning: Chemical, topographical, and biological analysis.
The objective of this study was to produce fibrous biomaterials with cell adhesive and cell repulsive capabilities for biomedical applications. To this aim, the surface of 100-mum diameter polyethylene terephthalate fibers were functionalized with acetaldehyde plasma polymer deposition followed by carboxymethyl dextran grafting onto the aldehyde-coated surfaces via a polyethyleneimine interlayer. The performance of the surface modification steps were confirmed by surface chemical composition analysis using X-ray photoelectron spectroscopy, surface topography analysis by atomic force microscopy, and scanning electron microscopy. The acetaldehyde plasma polymer-coated and polyethyleneimine-grafted substrates promoted human umbilical vein endothelial cells attachment, spreading and actin filaments/focal adhesions formation. In contrast, carboxymethyl dextran-grafted substrates resisted cell adhesion. These observations demonstrate that the current surface-modified polymer fibers can be used in tissue engineering applications, such as cell patterning substrates or vascular prosthesis development.
Department of Chemical Engineering and Biotechnology, University of Sherbrooke, 2500 University Boulevard, Sherbrooke, Québec, J1K 2R1, Canada. afra.hajizadeh@gmail.com
Afra
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Hadjizadeh
D000079:Acetaldehyde / Q000737:chemistry*; D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D022001:Focal Adhesions / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D014471:Umbilical Veins / Q000166:cytology
D000199:Actins; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D011108:Polymers; D011094:Polyethyleneimine; D000079:Acetaldehyde