[Agrobacterium-mediated in Planta transformation of maize germ cells].
Abstract-A transfer DNA (T-DNA) carrying the marker gene nptII was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of Agrobacterium during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds). The example of matroclinal haploids was used to demonstrate that T-DNA may be transported to the egg cell by the growing pollen tube (PT). Twelve out of 16 analyzed haploid plants contained the T-DNA insertion. The possible mechanism of the transfer of the Agrobacterium T-DNA to the maize genome during pollination is discussed.
Mamontova
E M
EM
Velikov
V A
VA
Volokhina
I V
IV
Chumakov
M I
MI
D004269:DNA, Bacterial / Q000235:genetics*; D004171:Diploidy; D035264:Flowers / Q000166:cytology / Q000235:genetics; D006238:Haploidy; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics*; D012231:Rhizobium; D014170:Transformation, Genetic; D003313:Zea mays / Q000166:cytology / Q000235:genetics*
D004269:DNA, Bacterial; C036483:T-DNA