Structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase (isoform A4) from Arabidopsis thaliana in complex with NAD.
The crystal structure of the A(4) isoform of photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Arabidopsis thaliana, expressed in recombinant form and complexed with NAD, is reported. The crystals, which were grown in 2.4 M ammonium sulfate and 0.1 M sodium citrate, belonged to space group I222. The asymmetric unit includes ten subunits, i.e. two independent tetramers plus a dimer that generates a third tetramer by a crystallographic symmetry operation. The crystal structure was solved by molecular replacement and refined to an R factor of 23.7% and an R(free) factor of 28.9% at 2.6 A resolution. In the final model, each subunit binds one NAD(+) molecule and two sulfates, which occupy the P(s) and the P(i) anion-binding sites. Detailed knowledge of this structure is instrumental for structural investigation of supramolecular complexes of A(4)-GAPDH, phosphoribulokinase and CP12, which are involved in the regulation of photosynthesis in the model plant A. thaliana.
Fermani
Simona
S
Department of Chemistry, University of Bologna, Via Selmi 2, 40126 Bologna, Italy.
Sparla
Francesca
F
Marri
Lucia
L
Thumiger
Anton
A
Pupillo
Paolo
P
Falini
Giuseppe
G
Trost
Paolo
P
D017360:Arabidopsis / Q000201:enzymology*; D018360:Crystallography, X-Ray; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry; D008958:Models, Molecular; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry; D018724:Spinacia oleracea / Q000737:chemistry; D040681:Structural Homology, Protein
D007527:Isoenzymes; D021122:Protein Subunits; D009243:NAD; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases