Reduced mitochondrial properties in putative progenitor/stem cells of human keratinocytes.
BACKGROUND
The characterization of progenitor/keratinocyte stem cells (KSC) remains an unachieved goal. A previous study showed that rapid adhering cells to collagen IV had the characteristics of putative progenitor/KSCs.


OBJECTIVE
The purpose of this study was to investigate the genetic expression of rapid adhering cells compared to non adhering cells to determine the characteristic of KSCs.


METHODS
We isolated rapid adhering cells representative of KSCs from non adhering cells representative of transient amplifying cells. In addition, we differentiated cells from human tonsilar keratinocytes utilizing the adhering capability of the KSCs to collagen IV. Annealing control primer based differentially displayed polymerase chain reaction (PCR) was performed as well as Western blot analysis.


RESULTS
The levels of mitochondria-related gene expression were low in the rapid adhering cells compared to the non adhering cells. Mitochondrial complex I, COX IV, peroxiredoxins (I, II and IV) and mitochondrial membrane potential were all low in the rapid adhering cells compared to the non adhering cells.


CONCLUSIONS
Using an adhesion method on human collagen IV-coated plates, our results suggest that reduced mitochondrial function may be an important characteristic of KSCs.
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Sung-Eun
SE
Chang
Youngmi
Y
Kim Pak
Hae-Woong
HW
Lee
Jee-Ho
JH
Choi
Eun-Jeong
EJ
Jeong
Seung-Ho
SH
Choi
Hyo Won
HW
Chang
Yoo-Sam
YS
Chung
Sang Yoon
SY
Kim