Dynamics of memory and naïve CD8+ T lymphocytes in humanized NOD/SCID/IL-2Rgammanull mice infected with CCR5-tropic HIV-1.
Creating a novel small animal model of HIV-1 infection that can support long-term systemic HIV-1 infection and produce HIV-1-specific immune response has a great benefit for studying HIV-1 pathogenesis in vivo. In the present study, we have generated a humanized mouse, NOG-hCD34 mouse, by transplanting newborn NOD/SCID/IL-2Rgamma(null) mice with human hematopoietic stem cells through hepatic injection. These mice were infected with a CCR5-tropic HIV-1 and were analyzed for plasma viral load, changes in peripheral blood T lymphocytes, and HIV-1-specific antibody production. High level of viral replication, increase in effector/memory CD8(+) T lymphocytes, class-switching to IgG, and production of HIV-1-specific IgGs were observed. Our findings suggest that NOG-hCD34 mice may have a wide variety of application in HIV-1 research.
Sato
Kei
K
Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
Nie
Chuanyi
C
Misawa
Naoko
N
Tanaka
Yuetsu
Y
Ito
Mamoru
M
Koyanagi
Yoshio
Y
D000818:Animals; D000917:Antibody Formation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D036101:Cord Blood Stem Cell Transplantation; D004195:Disease Models, Animal; D015483:HIV Antibodies / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D017578:Immunoglobulin Class Switching; D007074:Immunoglobulin G / Q000276:immunology; D007156:Immunologic Memory; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D012367:RNA, Viral / Q000097:blood; D019713:Receptors, CCR5 / Q000276:immunology; D019562:Viral Load; D014766:Viremia; D014779:Virus Replication
D015483:HIV Antibodies; D007074:Immunoglobulin G; D053631:Interleukin Receptor Common gamma Subunit; D012367:RNA, Viral; D019713:Receptors, CCR5