Determination of food grade antioxidants using microemulsion electrokinetic chromatography.
Seven food grade antioxidants were resolved by microemulsion electrokinetic chromatography. The antioxidants included propyl gallate, octyl gallate, dodecyl gallate, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone and alpha-tocopherol. They were resolved in 11 min using a low pH microemulsion containing 20% propan-2-ol. Theoretical plates in the range 39,000-95,000 per length of column (38.5 cm) were recorded for the antioxidants. The LODs were calculated to be in the range 9-44 microg/mL (30 s hydrostatic injection and a 50 microm id capillary column). The method can also be used to simultaneously analyse for the synthetic preservatives sorbic acid, benzoic acid, salicylic acid and the parabens. The method was successfully applied to noodles for the determination of butylated hydroxytoluene and propyl gallate and to pharmaceutical supplement tablets for alpha-tocopherol. The in-capillary concentration of butylated hydroxytoluene by sweeping was also demonstrated.
School of Natural Sciences, Edith Cowan University, WA, Australia.
Vishal
V
Darji
Mary C
MC
Boyce
Ian
I
Bennett
Michael C
MC
Breadmore
Joselito
J
Quirino
D019840:2-Propanol / Q000737:chemistry; D000975:Antioxidants / Q000032:analysis*; D002084:Butylated Hydroxytoluene / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004655:Emulsions; D005504:Food Analysis / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D024502:alpha-Tocopherol / Q000032:analysis
D000975:Antioxidants; D004655:Emulsions; D005520:Food Preservatives; D002084:Butylated Hydroxytoluene; D005707:Gallic Acid; D024502:alpha-Tocopherol; D019840:2-Propanol