Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles.
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Jiraphan
J
Junjhon
Thomas J
TJ
Edwards
Utaiwan
U
Utaipat
Valorie D
VD
Bowman
Heather A
HA
Holdaway
Wei
W
Zhang
Poonsook
P
Keelapang
Chunya
C
Puttikhunt
Rushika
R
Perera
Paul R
PR
Chipman
Watchara
W
Kasinrerk
Prida
P
Malasit
Richard J
RJ
Kuhn
Nopporn
N
Sittisombut
D020285:Cryoelectron Microscopy; D003716:Dengue Virus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D047468:Immunoprecipitation; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure*; D019065:Virus Assembly
D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus