Splice-variant 1 of the ancient domain protein 2 (ACDP2) complements the magnesium-deficient growth phenotype of Salmonella enterica sv. typhimurium strain MM281.
Evidence arguing for the existence of genes encoding for proteins directly involved in the transport of Mg2+ through the cytoplasmic membrane have accumulated over the last few years. Gene ACDP2 (ancient conserved domain protein 2; old name CNNM2, cyclin M2) is one such gene. ACDP2 is a distant homologue of the bacterial gene corC, which is known to be involved in cobalt resistance. We have previously demonstrated that the over-expression of the human Mg2+ carrier SLC41A1 partly complements the Mg2+-dependent growth deficiency of Salmonella strain MM281 (triple disruptant in genes: mgtA, mgtB and corA) cultivated in media containing growth non-permissive [Mg2+]e. We have used the same approach to examine whether over-expressed human ACDP2 has a similar efficacy to complement growth deficiency of the MM281 strain in media containing growth non-permissive [Mg2+]e. Two splicing variants of the ACDP2 gene have been tested. Here, we show that over-expressed isomorph 1 is efficient in restoring growth of the MM281 strain in media containing growth non-permissive [Mg2+]e, whereas isomorph 2 is not. Therefore, we conclude that ACDP2sp.v.1 is a functional Mg2+-transporting entity per se. Our conclusion is supported by the measurable Mg2+ influx seen in MM281 bacteria over-expressing ACDP2sp.v.1 but not in MM281 bacteria over-expressing ACDP2sp.v.2 or in cells transformed with the empty vector.
Max F. Perutz Laboratories, Department of Microbiology, Immunobiology and Genetics, Campus Vienna Biocenter, University of Vienna, Wien, Austria.
Gerhard
G
Sponder
Sona
S
Svidova
Monika
M
Schweigel
Jürgen
J
Vormann
Martin
M
Kolisek
D017398:Alternative Splicing / Q000187:drug effects / Q000235:genetics*; D001692:Biological Transport / Q000187:drug effects; D027682:Cation Transport Proteins; D003470:Culture Media / Q000494:pharmacology; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism*; D016257:Fura-2 / Q000378:metabolism; D005816:Genetic Complementation Test; D006801:Humans; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology; D010641:Phenotype; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000254:growth & development*
C558175:CNNM2 protein, human; D027682:Cation Transport Proteins; D003470:Culture Media; D016213:Cyclins; D008274:Magnesium; D016257:Fura-2