Acetaldehyde plasma polymer-coated PET fibers for endothelial cell patterning: Chemical, topographical, and biological analysis.
The objective of this study was to produce fibrous biomaterials with cell adhesive and cell repulsive capabilities for biomedical applications. To this aim, the surface of 100-mum diameter polyethylene terephthalate fibers were functionalized with acetaldehyde plasma polymer deposition followed by carboxymethyl dextran grafting onto the aldehyde-coated surfaces via a polyethyleneimine interlayer. The performance of the surface modification steps were confirmed by surface chemical composition analysis using X-ray photoelectron spectroscopy, surface topography analysis by atomic force microscopy, and scanning electron microscopy. The acetaldehyde plasma polymer-coated and polyethyleneimine-grafted substrates promoted human umbilical vein endothelial cells attachment, spreading and actin filaments/focal adhesions formation. In contrast, carboxymethyl dextran-grafted substrates resisted cell adhesion. These observations demonstrate that the current surface-modified polymer fibers can be used in tissue engineering applications, such as cell patterning substrates or vascular prosthesis development.
Hadjizadeh
Afra
A
Department of Chemical Engineering and Biotechnology, University of Sherbrooke, 2500 University Boulevard, Sherbrooke, Québec, J1K 2R1, Canada. afra.hajizadeh@gmail.com
D000079:Acetaldehyde / Q000737:chemistry*; D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D022001:Focal Adhesions / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D056951:Photoelectron Spectroscopy; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D014471:Umbilical Veins / Q000166:cytology
D000199:Actins; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D011108:Polymers; D011094:Polyethyleneimine; D000079:Acetaldehyde