Polyhydroxy steroids and saponins from China Sea starfish Asterina pectinifera and their biological activities.
A new polyhydroxy sterol ester, (25S)-5alpha-cholestane-3beta,6alpha,7alpha,8,15alpha,16beta-hexahydroxyl-26-O-14'Z-eicosenoate (1), together with seven known steroid derivatives (2-8), were isolated from the EtOH extract of the whole body of China Sea starfish Asterina pectinifera. The structure of 1 was determined by using extensive spectra analysis (IR, 1D and 2D NMR, and MS), chemical degradation, and comparison with the known compound (25S)-5alpha-cholestane-3beta,6alpha,7alpha,8,15alpha,16beta,26-heptol (2). All the isolates were evaluated for their antiviral activity against herpes simplex virus type 1 (HSV-1) and their cytotoxicity against human liver carcinoma HepG2 cell line in vitro. Compounds 3-6, and 8 exhibited antiviral activity against HSV-1 virus with the minimal inhibitory concentration (MIC) values of 0.2, 0.05, 0.2, 0.22, and 0.07 microM, respectively. While compounds 4 and 5 exhibited cytotoxicity against HepG2 cells with IC(50) values of 0.2 and 1.6 microM, respectively.
Peng
Yan
Y
College of Light Industry and Food Sciences, South China University of Technology.
Zheng
Jianxian
J
Huang
Riming
R
Wang
Yifei
Y
Xu
Tunhai
T
Zhou
Xuefeng
X
Liu
Qiuying
Q
Zeng
Fanli
F
Ju
Huaiqiang
H
Yang
Xianwen
X
Liu
Yonghong
Y
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D047288:Asterina / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D056945:Hep G2 Cells; D006561:Herpes Simplex / Q000188:drug therapy*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000998:Antiviral Agents; D012503:Saponins; D013256:Steroids