Transcriptome analysis by cDNA-AFLP of Suillus luteus Cd-tolerant and Cd-sensitive isolates.
The ectomycorrhizal basidiomycete Suillus luteus (L.:Fr.), a typical pioneer species which associates with young pine trees colonizing disturbed sites, is a common root symbiont found at heavy metal contaminated sites. Three Cd-sensitive and three Cd-tolerant isolates of S. luteus, isolated respectively from non-polluted and a heavy metal-polluted site in Limburg (Belgium), were used for a transcriptomic analysis. We identified differentially expressed genes by cDNA-AFLP analysis. The possible roles of some of the encoded proteins in heavy metal (Cd) accumulation and tolerance are discussed. Despite the high conservation of coding sequences in S. luteus, a large intraspecific variation in the transcript profiles was observed. This variation was as large in Cd-tolerant as in sensitive isolates and may help this pioneer species to adapt to novel environments.
Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, 3590 Diepenbeek, Belgium.
Joske
J
Ruytinx
Adrian R
AR
Craciun
Karen
K
Verstraelen
Jaco
J
Vangronsveld
Jan V
JV
Colpaert
Nathalie
N
Verbruggen
D054458:Amplified Fragment Length Polymorphism Analysis; D001487:Basidiomycota / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D018076:DNA, Complementary; D005656:Fungal Proteins; D012989:Soil Pollutants; D002104:Cadmium