Mycorrhizal symbionts of Pisonia grandis and P. sechellarum in Seychelles: identification of mycorrhizal fungi and description of new Tomentella species.
Nyctaginaceae includes species that are predominantly non-mycorrhizal or form arbuscular or ectomycorrhiza. Root-associated fungi were studied from P. grandis and P. sechellarum roots collected respectively on the islands of Cousin and Silhouette in Seychelles. In addition fungal sporocarps were collected from the sampling area. Fungal symbionts were identified from the roots by anatomotyping and rDNA sequencing; sporocarps collected were examined microscopically and sequenced. Three distantly related ectomycorrhizal fungal species belonging to Thelephoraceae were identified from the roots of P. grandis. Sporocarps also were found for two symbionts and described as new Tomentella species. In addition Tomentella species collected from other Seychelles islands were studied and described as new species if there was no close resemblance to previously established species. P. sechellarum was determined to be an arbuscular mycorrhizal plant; three arbuscular mycorrhizal fungal species were detected from the roots. P. grandis is probably associated only with species of Thelephoraceae throughout its area. Only five Tomentella species are known to form ectomycorrhiza with P. grandis and they never have been found to be associated with another host, suggesting adaptation of these fungi to extreme environmental conditions in host's habitat.
Institute of Ecology and Earth Sciences, University of Tartu, 14A Ravila Street, 50411 Tartu, Estonia. triin.suvi@gmail.com
Triin
T
Suvi
Leho
L
Tedersoo
Kessy
K
Abarenkov
Katy
K
Beaver
Justin
J
Gerlach
Urmas
U
Kõljalg
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D039281:Nyctaginaceae / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012750:Seychelles; D013045:Species Specificity; D013172:Spores, Fungal / Q000254:growth & development; D013559:Symbiosis
D004271:DNA, Fungal