Separation of benzyl derivatives of germanium for HPLC.
The paper presents interactions of mono-, di-, tri-, and tetrabenzyl derivatives of germanium with stationary phase surface and results from these processes of determination. As a final result of the work, optimal conditions of chromatographic separation and determination of these compounds by high-performance liquid chromatography (HPLC) method are proposed. During investigations, different types of stationary phase (including octadecyl, octyl, and chemically bonded aryl phases) and mobile phases are considered. As a result of the investigation, the highest selectivity is showed chemically by bonded aryl stationary phase combined with a mobile phase consisting of acetonitrile (capacity factors of di-, tri-, and tetrabenzyl derivatives of germanium k(1) = 0.86, k(2) = 2.53, k(3) = 6.45). However, octadecyl phase, which is considered as reference phase, exhibits the lowest separation factors of determined benzylgermanium compounds (k(1) = 9.12, k(2) = 37.62, k(3) = 70.43).
Institute of Chemistry, University of Podlasie, Siedlce, Poland. kluskam@ap.siedlce.pl
Mariusz
M
Kluska
Krzysztof
K
Pypowski
Ireneusz
I
Chrzascik
Monika
M
Michel
Nikolai
N
Erchak