Expression profile of PIN, AUX/LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress.
Auxin is transported by the influx carriers auxin resistant 1/like aux1 (AUX/LAX), and the efflux carriers pin-formed (PIN) and P-glycoprotein (PGP), which play a major role in polar auxin transport. Several auxin transporter genes have been characterized in dicotyledonous Arabidopsis, but most are unknown in monocotyledons, especially in sorghum. Here, we analyze the chromosome distribution, gene duplication and intron/exon of SbPIN, SbLAX and SbPGP gene families, and examine their phylogenic relationships in Arabidopsis, rice and sorghum. Real-time PCR analysis demonstrated that most of these genes were differently expressed in the organs of sorghum. SbPIN3 and SbPIN9 were highly expressed in flowers, SbLAX2 and SbPGP17 were mainly expressed in stems, and SbPGP7 was strongly expressed in roots. This suggests that individual genes might participate in specific organ development. The expression profiles of these gene families were analyzed after treatment with: (a) the phytohormones indole-3-acetic acid and brassinosteroid; (b) the polar auxin transport inhibitors 1-naphthoxyacetic acids, 1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid; and (c) abscissic acid and the abiotic stresses of high salinity and drought. Most of the auxin transporter genes were strongly induced by indole-3-acetic acid and brassinosteroid, providing new evidence for the synergism of these phytohormones. Interestingly, most genes showed similar trends in expression under polar auxin transport inhibitors and each also responded to abscissic acid, salt and drought. This study provides new insights into the auxin transporters of sorghum.
State Key Laboratory of Plant Physiology and Biochemistry, Zhejiang University, Hangzhou, China.
ChenJia
C
Shen
YouHuang
Y
Bai
SuiKang
S
Wang
SaiNa
S
Zhang
YunRong
Y
Wu
Ming
M
Chen
DeAn
D
Jiang
YanHua
Y
Qi
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000737:chemistry / Q000235:genetics*; D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics; D002874:Chromosome Mapping; D019295:Computational Biology; D003681:Dehydration / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D005091:Exons / Q000235:genetics; D020440:Gene Duplication; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D006016:Glycolates / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007438:Introns / Q000235:genetics; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D012275:Oryza / Q000235:genetics; D010797:Phthalimides / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics; D018514:Plant Structures / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D054712:Salinity; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology; D045868:Sorghum / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D014283:Triiodobenzoic Acids / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics
D018435:ATP Binding Cassette Transporter, Subfamily B; D006016:Glycolates; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010797:Phthalimides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014283:Triiodobenzoic Acids; C002687:alpha-naphthylphthalamic acid; D012965:Sodium Chloride; C030737:indoleacetic acid; D000040:Abscisic Acid; C018610:1-naphthoxyacetic acid; C052968:2,3,5-triiodobenzoic acid