Hydrophobic protein in colorectal cancer in relation to tumor stages and grades.
OBJECTIVE
To identify differentially expressed hydrophobic proteins in colorectal cancer.


METHODS
Eighteen pairs of colorectal cancerous tissues in addition to tissues from normal mucosa were analysed. Hydrophobic proteins were extracted from the tissues, separated using 2-D gel electrophoresis and analysed using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS). Statistical analysis of the proteins was carried out in order to determine the significance of each protein to colorectal cancer (CRC) and also their relation to CRC stages, grades and patients' gender.


RESULTS
Thirteen differentially expressed proteins which were expressed abundantly in either cancerous or normal tissues were identified. A number of these proteins were found to relate strongly with a particular stage or grade of CRC. In addition, the association of these proteins with patient gender also appeared to be significant.


CONCLUSIONS
Stomatin-like protein 2 was found to be a promising biomarker for CRC, especially in female patients. The differentially expressed proteins identified were associated with CRC and may act as drug target candidates.
School of Pharmaceutical Sciences, Universiti Sains Malaysia, USM 11800, Penang, Malaysia.
Lay-Chin
LC
Yeoh
Chee-Keat
CK
Loh
Boon-Hui
BH
Gooi
Manjit
M
Singh
Lay-Harn
LH
Gam
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D001798:Blood Proteins / Q000737:chemistry; D002853:Chromatography, Liquid; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry*; D009367:Neoplasm Staging; D040081:Protein Array Analysis; D053719:Tandem Mass Spectrometry
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C406287:STOML2 protein, human