Capsaicin, a component of red peppers, stimulates protein kinase CKII activity.
Protein kinase CKII (CKII), a heterotetramer composed of two catalytic (alpha or alpha') subunits and two regulatory (beta) subunits, plays a critical role in cell proliferation and anti-apoptosis. Recently, capsaicin was shown to trigger apoptosis. Therefore, we examined the effect of capsaicin on CKII activity. Although capsaicin induced apoptotic death in HeLa cells, CKII activity was increased in the cytosolic fraction of HeLa cells after treatment. Capsaicin did not change the expression of the CKIIalpha and CKIIbeta proteins. Capsaicin stimulated the catalytic activity of recombinant CKII tetramer, but not the CKIIalpha subunit. Moreover, capsaicin enhanced the autophosphorylation of CKIIalpha and CKIIbeta. Taken together, our data suggest that capsaicin stimulates the phosphotransferase activity of CKII holoenzyme by interacting with the CKIIbeta subunit.
Kyungpook National University, Daegu, Korea.
Yun-Wha
YW
Rho
Young-Seuk
YS
Bae
D002211:Capsaicin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002212:Capsicum / Q000737:chemistry*; D047390:Casein Kinase II / Q000378:metabolism*; D004789:Enzyme Activation; D006367:HeLa Cells / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D015394:Molecular Structure; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*
D021122:Protein Subunits; D011065:Poly(ADP-ribose) Polymerases; D047390:Casein Kinase II; D002211:Capsaicin