Development of an improved protocol for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula.
Enterobacter sakazakii causes severe maladies and, in some cases, is fatal among infants. Powdered infant formula (PIF) contaminated with E. sakazakii has been documented as a potential cause of several outbreaks involving infants. This study describes the development of a method for the isolation and detection of E. sakazakii from PIF. It combines Taqman real-time PCR, Brilliance E. sakazakii and R&F chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 1 to 2 days depending on the amount and stress status of E. sakazakii organisms and competing microorganisms in PIF. The real-time PCR has bifunctional applications, including both screening and culture confirmation of E. sakazakii.
U.S. Food and Drug Administration, 5100 Paint Branch Parkway HFS-711, College Park, Maryland 20740, USA.
Yi
Y
Chen
Kwang-Yong
KY
Song
Eric W
EW
Brown
Keith A
KA
Lampel
D003257:Consumer Product Safety; D044083:Cronobacter sakazakii / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000382:microbiology*; D041943:Infant Formula; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial