Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.
Establishment of connectivity between peripheral and central organs is essential for sensory processing by dorsal root ganglion (DRG) neurons. Using Ret as a marker for mechanoreceptive DRG neurons, we show that both central and peripheral projections of mechanoreceptive neurons are severely impaired in the absence of Ret. Death of DRG neurons in Ret-deficient mice can be rescued by eliminating Bax, although their projections remain disrupted. Furthermore, ectopic expression of the Ret ligand neurturin, but not Gdnf, in the spinal cord induces aberrant projection of mechanoreceptive afferents. Our results demonstrate that Ret expression in DRG neurons is crucial for the neurturin-mediated formation of precise axonal projections in the central nervous system.
Honma
Yutaka
Y
Ogawa Research Unit, Brain Science Institute, RIKEN, Saitama 351-0198, Japan. yhonma@brain.riken.jp
Kawano
Masako
M
Kohsaka
Shinichi
S
Ogawa
Masaharu
M
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000378:metabolism / Q000502:physiology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D051101:Neurturin / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism
D051100:Glial Cell Line-Derived Neurotrophic Factor; D051101:Neurturin