Functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis.
Phosphatidylcholine-specific phospholipase C (PC-PLC) is the major enzyme in the Phosphatidylcholine (PC) cycle and is involved in many long-term cellular responses such as activation, proliferation, and differentiation events. Cell division cycle 20 homolog (Cdc20) is an essential cell-cycle regulator required for the completion of mitosis. Our previous studies identified the interaction between PC-PLC and Cdc20. Through the interaction, Cdc20 could mediate the degradation of PC-PLC by Cdc20-mediated ubiquitin proteasome pathway (UPP). In this study, we found that PC-PLC might not be involved in cancer metastasis. Inhibition of PC-PLC by D609 could cause cell proliferation inhibition and apoptosis inhibition in CBRH-7919 cells. Inhibition of PC-PLC could also influence the cell cycle by arresting the cells in G1 phase, and Cdc20 might be involved in these processes. Taken together, in this report, we provided new evidence for the functional roles of PC-PLC and Cdc20 in the cell cycle, proliferation, and apoptosis in CBRH-7919 cells.
Shanghai Lung Tumor Clinical Medical Center, Shanghai Chest Hospital, Shanghai Jiaotong University, PR China.
Zhiwei
Z
Chen
Yongfeng
Y
Yu
Da
D
Fu
Ziming
Z
Li
Xiaoming
X
Niu
Meilin
M
Liao
Shun
S
Lu
D000818:Animals; D017209:Apoptosis; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000494:pharmacology; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D016193:G1 Phase; D006801:Humans; D007976:Leupeptins / Q000737:chemistry / Q000494:pharmacology; D009636:Norbornanes; D051381:Rats; D013859:Thiocarbamates; D013871:Thiones / Q000737:chemistry / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism / Q000502:physiology*
D001952:Bridged-Ring Compounds; D064199:Cdc20 Proteins; C577494:Cdc20 protein, rat; D018797:Cell Cycle Proteins; D007976:Leupeptins; D009636:Norbornanes; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde