Effects of genetic captive-breeding protocols on sperm quality and fertility in the white-footed mouse.
Mice (Peromyscus leucopus noveboracensis) from a captive-breeding program were used to test the effects of three genetic breeding protocols (minimizing mean kinship [MK], random breeding, and selection for docility [DOC]) and inbreeding levels on sperm traits and fertility. Earlier, in generation 8, one DOC replicate went extinct because of poor reproductive success. By generation 10, spermatozoa from DOC mice had more acrosome and midpiece abnormalities, which were shown to be strong determinants of fertility, as well as lower sperm production and resistance to osmotic stress. In addition, determinants of fertility, including male and female components, were assessed in a comprehensive manner. Results showed that the probability (P) of siring litters is determined by sperm number, sperm viability, and midpiece and acrosome abnormalities; that the P of siring one versus two litters is determined by tail abnormalities; and that the total number of offspring is influenced by female size and proportion of normal sperm, showing the relative importance of different sperm traits on fertility. On average, males with 20% normal sperm sired one pup per litter, and males with 70% normal sperm sired eight pups per litter. Interestingly, the proportion of normal sperm was affected by docility but not by relatively low inbreeding. However, inbreeding depression in sperm motility was detected. In the MK group, inbreeding depression not only affected sperm motility but also fertility: An increase in the coefficient of inbreeding (f) of 0.03 reduced sperm motility by 30% and translated into an offspring reduction of three pups in second litters. A genetic load of 48 fecundity equivalents was calculated.
Center for Conservation and Evolutionary Genetics, National Zoological Park, Smithsonian Institution, Washington, DC, USA a.malo@imperial.ac.uk
Aurelio F
AF
Malo
Felipe
F
Martinez-Pastor
Glen
G
Alaks
Jean
J
Dubach
Robert C
RC
Lacy
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000502:physiology; D005260:Female; D005298:Fertility / Q000502:physiology*; D007178:Inbreeding / Q000379:methods*; D008098:Litter Size; D016015:Logistic Models; D008297:Male; D051379:Mice; D009929:Organ Size / Q000502:physiology; D010542:Peromyscus; D011247:Pregnancy; D011897:Random Allocation; D012641:Selection, Genetic / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology*; D013737:Testis / Q000502:physiology*