Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.
DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.
Igarashi
Ryuji
R
Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Sakai
Tomomi
T
Hara
Hideyuki
H
Tenno
Takeshi
T
Tanaka
Toshiaki
T
Tochio
Hidehito
H
Shirakawa
Masahiro
M
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D008958:Models, Molecular; D009865:Oocytes; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels; D025801:Ubiquitin / Q000737:chemistry; D014982:Xenopus laevis
D011506:Proteins; D013113:Spin Labels; D025801:Ubiquitin