Hypoglycaemic effects of fermented mycelium of Paecilomyces farinosus (G30801) on high-fat fed rats with streptozotocin-induced diabetes.
OBJECTIVE
Paceilomyces farinosus is an entomogenous fungus with a powerful insecticidal activity against the larvae of Lipidoptera, Coleoptera and Hymenoptera. However, the hypoglycaemic activity of P. farinosus extract has not been studied. This study was undertaken to investigate the hypoglycaemic and anti-diabetic effects of P. farinosus (G30801) in rats with streptozotocin (STZ)-induced diabetes given a high-fat and compared with normal rats.


METHODS
Rats fed with high fat diet for 2 months and injected with (30 or 50 mg STZ/kg bw) showed raised level of plasma triglyceride (TG), cholesterol, D-glucose concentration and glycosylated haemoglobin (HbA1C) %. The STZ-induced type 1 (T1DM) and type 2 diabetes (T2DM) in rats was further confirmed using glucose tolerance test and insulin-glucose tolerance test. P. farinosus (G30801) was fermented in different media [soybean (S), black bean (B), and rice (R)] and their extracts were tested for hypoglycaemic effect using T1DM and T2DM rats.


RESULTS
STZ (30 and 50 mg/kg bw) could successfully induce T2DM and T1DM in rats, respectively. No change in blood glucose levels were noted in P. farinosus (R medium) treated normal rats (P < 0.05). In addition, STZ-high fat fed diabetic (T1DM and T2DM) rats when treated with P. farinosus (R medium) showed decreased blood glucose level as compared with P. farinosus extracted from B and S medium.


CONCLUSIONS
Our findings showed hypoglycaemic effect of fermented P. farinosus (G30801) in experimental diabetes rat model fed with high fat diet.
Institute of Biotechnology, National Dong-Hwa University, Hualien, Taiwan.
Huai-En
HE
Lu
Chien-Huei
CH
Jian
Shu-Fen
SF
Chen
Tse-Min
TM
Chen
Son-Tay
ST
Lee
Chun-Sheng
CS
Chang
Ching-Feng
CF
Weng
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D004041:Dietary Fats / Q000008:administration & dosage*; D005285:Fermentation; D005951:Glucose Tolerance Test; D010143:Paecilomyces / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
D004041:Dietary Fats; D013311:Streptozocin