Cloning and characterization of bovine interleukin-32 beta isoform.
Interleukin-32 (IL-32) is a new cytokine produced mainly by T-cells, natural killer cells, and epithelial cells after stimulation with IL-2, IL-12 plus IL-18, and IFNgamma, respectively. IL-32 induces various proinflammatory cytokines such as TNFalpha, IL-1beta, IL-6, and IL-8 in monocytes and macrophages. In this study, we cloned bovine IL-32 cDNA from peripheral blood mononuclear cells (PBMC) of a Holstein (Bos Taurus). The bovine IL-32 cDNA has an entire open reading frame, encoding 171 amino acid residues and the deduced amino acid sequence has 27.5% identity with human IL-32 beta isoform. Recombinant bovine IL-32 protein was produced in E. coli and its molecular weight was approximately 26kDa. The biological activity of the bovine IL-32 was examined for cytokine induction using human monocytic THP-1 cells and bovine PBMC. The THP-1 cells responded to stimulation by recombinant bovine IL-32 and produced IL-8. Stimulation by recombinant bovine IL-32 induced mRNA for TNFalpha and IL-6 in bovine PBMC suggesting conservation of the biological activity and function in cattle.
Jaekal
Jun
J
Laboratory of Cytokine Immunology, Medical Immunology Center, Institute of Biomedical Science and Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Jhun
Hyunjhung
H
Hong
Jaewoo
J
Park
Seungyoung
S
Lee
Joongbok
J
Yoon
Doyoung
D
Lee
Siyoung
S
Her
Erk
E
Yang
Young
Y
Rho
Gyujin
G
Kim
Soohyun
S
D000818:Animals; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D020033:Protein Isoforms; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
C496971:IL32 protein, human; D007378:Interleukins; D020033:Protein Isoforms; D011994:Recombinant Proteins