Sp1 plays an important role in regulating the transcription of ZNF313.
The ZNF313 gene has the highest transcription level in fertile male testes and may be related to human spermatogenesis. The deletion-mutated plasmids of ZNF313 promoter were constructed and transfected into HEK293 cells. The result showed that the fragment from nt -157 to +8 has a basal transcriptional activity. A functional Sp1 binding site was identified by site-directed mutation test and mithramycin A treatment. A 447-bp based at +233 to -213 exhibits a characteristic CpG island, which overlaps with the promoter region. Our work suggests that ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists.
Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section3#17, Chengdu 610041, Peoples Republic of China.
Xiaolin
X
Liao
Wenqian
W
Deng
Yilu
Y
Lu
Dachang
D
Tao
Yunqiang
Y
Liu
Sizhong
S
Zhang
Yongxin
Y
Ma
D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D018899:CpG Islands / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D016329:Sp1 Transcription Factor; C477146:RNF114 protein, human; D044767:Ubiquitin-Protein Ligases