Increased expression of glucose transporter 3 in gerbil brains following magnesium sulfate treatment and focal cerebral ischemic injury.
Glucose is the primary energy substrate for neurons. Glucose transporter 3 (Glut3) localizes at the neuronal cellular membrane, which transports glucose from the extracelluar space into neurons. Ischemia results in an increased energy demand that is associated with profound changes in brain energy metabolism. Magnesium sulfate (MgSO(4)) ameliorates ischemia-induced neuronal death in the rat and gerbil model. We investigated the effects of MgSO(4) administration on the expression of Glut3 in cortex and hippocampus of gerbils during ischemia. The focal cerebral ischemia was produced by unilateral occlusion of the right common carotid artery and right middle cerebral artery. Following ischemia, Glut3 expression increased significantly versus non-ischemic (contra-lateral) cortex and hippocampus. MgSO(4) treatment significantly increased the level of Glut3 expression in the non-ischemic and ischemic cortex and hippocampus. We found that the MgSO(4)-induced increase in Glut3 expression was not reversed by administration of U0126, a MEK kinase inhibitor. These results suggest that other factors may function to modulate the MgSO(4)-induced Glut3 response. In all, our data showed that MgSO(4) increases the expression of Glut3 in the cortex and hippocampus of gerbil brains both in non-ischemia and ischemia status. However, the MEK signaling pathway might not be involved in MgSO(4)-induced Glut3 expression following focal ischemia.
Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan.
Chih-Yang
CY
Huang
Yi-Fan
YF
Liou
Shu-Ying
SY
Chung
Pei-Ying
PY
Pai
Chung-Ben
CB
Kan
Chia-Hua
CH
Kuo
Chang-Hai
CH
Tsai
Fuu-Jen
FJ
Tsai
Jia-Long
JL
Chen
Jing-Ying
JY
Lin
D000818:Animals; D002545:Brain Ischemia / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D002070:Butadienes / Q000627:therapeutic use; D002540:Cerebral Cortex / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D005849:Gerbillinae; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D008278:Magnesium Sulfate / Q000633:toxicity; D008297:Male; D009570:Nitriles / Q000627:therapeutic use; D051381:Rats
D002070:Butadienes; D004791:Enzyme Inhibitors; D051274:Glucose Transporter Type 3; D009570:Nitriles; C113580:U 0126; D008278:Magnesium Sulfate