Characterization of the antiviral activity for influenza viruses M1 zinc finger peptides.
We sought to investigate the cellular uptake and antiviral activity for the M1 zinc finger peptides derived from influenza A and influenza B viruses in vitro. No cellular uptake was detected by fluorescent microscopy for the synthetic zinc finger peptides. When flanked to a cell permeable peptide Tp10, the zinc finger recombinant proteins were efficiently internalized by MDCK cells. However, no antiviral activity was detected against homologous or heterologous virus infections for the synthetic peptides or the Tp10-flanked recombinant proteins, regardless treated with or without Zn(2+). Nevertheless, MDCK cell constitutively expressing the M1 zinc finger peptides in cell nuclei potently inhibited replication of homologous, but not heterologous influenza viruses. Adenoviral vector delivered M1 zinc finger peptides also exhibited potent antiviral activity against homologous viruses challenge. Transduction at 100 PFU dose of recombinant adenovirus efficiently protected 99% of the cells from 100 TCID(50) of different virus infections for both peptides. These results brought new insight to the antiviral researches against influenza virus infections.
Laboratory of Wildlife Epidemic Diseases, East China Normal University, Shanghai, 200062, China. yjwang@ecnu.edu.cn
Yongjin
Y
Wang
Huihui
H
Xiao
Nannan
N
Wu
Huiling
H
Shi
Hongwei
H
Xu
Lichen
L
Zhou
Xu-Guang
XG
Xi
Tianhou
T
Wang
Xiaoming
X
Wang
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D005822:Genetic Vectors; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000254:growth & development*; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects; D016335:Zinc Fingers
D000998:Antiviral Agents; C545845:M1 matrix protein, influenza B virus; C488936:M1 protein, Influenza A virus; D010455:Peptides; D014763:Viral Matrix Proteins