Protective effect of Cyperi rhizoma against 6-hydroxydopamine-induced neuronal damage.
Cyperi rhizoma, the rhizome of Cyperus rotundus L. (Family Cyperaceae), is a well-known functional food and traditional herbal medicine in Korea. It has been reported that Cyperi rhizoma has antioxidant and free radical scavenging activities that play a major role in protection of neurodegenerative disorders, such as Parkinson's disease (PD). In the present study, the neuroprotective effects of a water extract of Cyperi rhizoma (CRE) against 6-hydroxydopamine (6-OHDA)-induced neuronal damage were evaluated in an experimental model of PD. In PC12 cells, CRE showed a significant protective effect on cell viability at 50 and 100 microg/mL. CRE inhibited generation of reactive oxygen species and nitric oxide, reduction of mitochondrial membrane potential, and caspase-3 activity, which were induced by 6-OHDA. CRE also showed a significant protective effect against damage to dopaminergic neurons in primary mesencephalic culture. These results suggest that CRE has neuroprotective effects against 6-OHDA-induced toxicity through antioxidant and anti-apoptotic activities in an in vitro PD model.
Lee
Chang-Hoon
CH
Department of Oriental Gynecology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Hwang
Deok-Sang
DS
Kim
Hyo Geun
HG
Oh
Hyein
H
Park
Hanbyeol
H
Cho
Jung-Hoon
JH
Lee
Jin-Moo
JM
Jang
Jun-Bock
JB
Lee
Kyung-Sub
KS
Oh
Myung Sook
MS
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003408:Cresols / Q000008:administration & dosage / Q000494:pharmacology*; D029786:Cyperus / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016627:Oxidopamine / Q000633:toxicity*; D016716:PC12 Cells; D010300:Parkinson Disease / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D010647:Phenyl Ethers / Q000008:administration & dosage / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry
D003408:Cresols; D018696:Neuroprotective Agents; D010647:Phenyl Ethers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C531339:cyperin; D016627:Oxidopamine