Molecular characterization and epidemiology of rotavirus isolates obtained from children with diarrhoea in Malaysia.
This retrospective study examined the G/P type of rotavirus in RNA samples that have previously been e-typed by RNA-PAGE in 1996. The results were then compared to 2007 samples to ascertain the extent of changes that may have occurred in this 11-years time interval. The G and P genotypes were determined by hemi-nested PCR and further analysed by phylogenetic study. In 1996, the G/P combination G1P[8], G(UT)P[8] and G1P(UT) prevalence rate were 81%, 9% and 7%, respectively. As expected, the G9 genotype which has already emerged worldwide was identified in 42% of the 2007 samples with the remaining 33% G1P[8] and 25% G1P(UT) Analysis of the RNA pattern showed that majority of the isolates were long e-type in both series, nevertheless minor differences within electropherotypes were observed. Genetic diversity in some strains of the human group A rotaviruses was analysed by phylogenetic methods. These findings will help in the decision to introduce rotavirus vaccines within the next decade.
Zuridah
H
H
Faculty of Health Sciences, Universiti Teknologi MARA, Jalan Othman, 46000 Petaling Jaya, Selangor, Malaysia. drzuridah@salam.uitm.edu.my
Kirkwood
C D
CD
Bishop
R F
RF
Bogdanovic-Sakran
N
N
Yap
K L
KL
D002648:Child; D003967:Diarrhea / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics* / Q000821:virology*; D013997:Time Factors
D012367:RNA, Viral