Global fitness profiling of fission yeast deletion strains by barcode sequencing.
A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.
Han
Tian Xu
TX
National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, PR China.
Xu
Xing-Ya
XY
Zhang
Mei-Jun
MJ
Peng
Xu
X
Du
Li-Lin
LL
D001483:Base Sequence; D002166:Camptothecin / Q000494:pharmacology; D016000:Cluster Analysis; D003470:Culture Media / Q000494:pharmacology; D001330:Electronic Data Processing; D017353:Gene Deletion; D020868:Gene Silencing / Q000187:drug effects; D056084:Genetic Fitness / Q000187:drug effects; D006238:Haploidy; D006918:Hydroxyurea / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology; D015203:Reproducibility of Results; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D017422:Sequence Analysis, DNA / Q000379:methods*; D013827:Thiabendazole / Q000494:pharmacology
D003470:Culture Media; D009153:Mutagens; D013827:Thiabendazole; D006918:Hydroxyurea; D002166:Camptothecin