Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates.
The complete nucleotide sequences of five isolates of poinsettia mosaic virus (PnMV) from Japan (JN, JO1, JO2, JO4, and JO5) were determined. These isolates contained a single large open reading frame in their genomes and shared 96.6-97.8% identity at the nucleotide level and 91.3-98.1% identity at the amino acid level with two previously reported European isolates. Interestingly, the JO isolates were found to possess eight common translational frameshift sites in the interdomain region between the methyltransferase and protease domains, resulting in considerable variation in the interdomain region compared to the other isolates. This suggests that PnMV might have evolved by creating variations in its genome by such translational frameshifts.
Okano
Yukari
Y
Laboratory of Clinical Plant Science, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Maejima
Kensaku
K
Shiraishi
Takuya
T
Hashimoto
Masayoshi
M
Senshu
Hiroko
H
Ozeki
Johji
J
Takahashi
Shuichiro
S
Komatsu
Ken
K
Yamaji
Yasuyuki
Y
Namba
Shigetou
S
D000595:Amino Acid Sequence; D028481:Euphorbia / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D046830:Tymoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D012324:RNA-Dependent RNA Polymerase