Excretory/secretory proteome of the adult stage of Echinostoma caproni.
The excretory/secretory proteome of Echinostoma caproni (Trematoda: Echinostomatidae) adults collected from experimentally infected mice was investigated using a proteomic approach. We performed a shot-gun liquid chromatography/tandem mass spectrometry for the separation and identification of tryptic peptides from the excretory/secretory products of E. caproni adult worms. Database search was performed using MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems). A total of 39 parasite proteins were accurately identified. Strikingly, metabolic enzymes, and particularly glycolytic enzymes, constituted the largest protein family in the excretory/secretory proteome of E. caproni adult worms. Moreover, representative proteins involved in parasite structure, response against stress, chaperones, calcium-binding, and signal transduction were also identified. This work extends our knowledge of host-parasite relationships in the E. caproni-rodent model that is extensively used to analyze the factors determining the intestinal helminth rejection. Consequently, information on many proteins may be useful to better understand the molecular basis that determines the survival of this parasite in the definitive host.
Sotillo
Javier
J
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, Burjassot, Valencia, Spain.
Valero
M Luz
ML
Sánchez Del Pino
Manuel M
MM
Fried
Bernard
B
Esteban
J Guillermo
JG
Marcilla
Antonio
A
Toledo
Rafael
R
D000818:Animals; D001700:Biomphalaria / Q000469:parasitology; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D004449:Echinostoma / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D004451:Echinostomiasis / Q000469:parasitology*; D020869:Gene Expression Profiling; D015801:Helminth Proteins / Q000737:chemistry / Q000378:metabolism*; D006790:Host-Parasite Interactions; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D020543:Proteome
D015801:Helminth Proteins; D020543:Proteome