Modulatory effect of Acetobacter xylinum cellulose on peritoneal macrophages.
BACKGROUND
The present work aimed to assess the effect of bacterial cellulose (BC) from Acetobacter xylinum on some functions of peritoneal macrophages.


METHODS
Mice peritoneal macrophages were lavaged and co-cultured with various concentrations of BC in microtiter plate. Viability of macrophages was determined by MTT assay. Nitric oxide (NO) amount was detected in culture supernatants of macrophages by Griess reagent and cytotoxicity effect of culture supernatants on WEHI-164 cells as tumor necrosis factor α (TNF-α) bioassay were done.


RESULTS
MTT assay for stimulated macrophages was not different (p = 0.486) and amount of NO production in all doses was the same as unstimulated control macrophages (p = 0.057). Supernatant of macrophages stimulated with 0.01 mg/mL of BC had cytotoxic effect on WEHI-164 fibrosarcoma cells (p = 0.040).


CONCLUSIONS
In this study, we showed that BC had no effect on macrophages viability and amount of NO production. BC suppressed the release of TNF-α from macrophages innate immune cells as indication of beneficial effect on wound healing. This data and further studies would imply use of this biomaterial for more applications.
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Saeed
S
Daneshmandi
Monire
M
Hajimoradi
Neda
N
Soleimani
Morteza
M
Sattari
D000818:Animals; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002482:Cellulose / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D005260:Female; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D008262:Macrophage Activation / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D017077:Culture Media, Conditioned; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D002482:Cellulose