ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes.
The Ada-Two-A-containing (ATAC) histone acetyltransferase and Mediator coactivator complexes regulate independent and distinct steps during transcription initiation and elongation. Here, we report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. Moreover, we identify leucine zipper motif-containing protein 1 as a subunit of this meta-coactivator complex (MECO). Finally, we demonstrate that the MECO regulates a subset of RNA polymerase II-transcribed non-coding RNA genes. Our findings establish that transcription coactivator complexes can form stable subcomplexes to facilitate their combined actions on specific target genes.
Department of Functional Genomics, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Parc d'Innovation, 1 rue Laurent Fries, CNRS UMR 7104, INSERM U 964, Université de Strasbourg, BP 10142, Illkirch Cedex 67404, France.
Arnaud R
AR
Krebs
Jeroen
J
Demmers
Krishanpal
K
Karmodiya
Nan-Chi
NC
Chang
Alice Chien
AC
Chang
László
L
Tora
D000818:Animals; D002478:Cells, Cultured; D019744:Chemokines, C / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005786:Gene Expression Regulation; D016350:Leucine Zippers; D013058:Mass Spectrometry; D056892:Mediator Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D046912:Multiprotein Complexes / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics*
D019744:Chemokines, C; D004268:DNA-Binding Proteins; C103593:Luzp1 protein, mouse; D056892:Mediator Complex; D046912:Multiprotein Complexes; D010455:Peptides; D021122:Protein Subunits; D022661:RNA, Untranslated; C486249:Xcl1 protein, mouse