Ultra-performance LC MS/MS method for quantification of clopidogrel active metabolite.
A sensible ultra-performance LC-MS method was developed and validated for the quantification of clopidogrel active metabolite in human plasma, with clopidogrel D4 as internal standard. Plasma pretreatment involved a one-step protein precipitation with acetonitrile. The separation was performed by reverse-phase chromatography on a C8 column. The method was linear over the concentration range of 1-150 ng/mL. The intra- and inter-day precision values were below 17% and accuracy was from 1.7 to 7.5% at all quality control levels. The lower LOQ was 0.8 ng/mL. Sample analysis time was reduced to 5 min including sample preparation (limited to protein precipitation). The present method was successfully applied to a clopidogrel active metabolite pharmacokinetic study following oral administration to healthy volunteers.
Laboratory of Pharmacology and Toxicology, University Hospital, Saint-Etienne, France. xavier.delavenne@chu-st-etienne.fr
Xavier
X
Delavenne
Thierry
T
Basset
Paul
P
Zufferey
Nora
N
Malouk
Silvy
S
Laporte
Patrick
P
Mismetti
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077144:Clopidogrel; D006801:Humans; D015394:Molecular Structure; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics
D000077144:Clopidogrel; D013988:Ticlopidine