Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis.
A novel on-plate digestion method combined with MALDI-MS analysis is reported, using trypsin-linked dendrimer-modified carbon nanotubes (dCNTs) as the enzyme immobilization probe. Excellent digestion performance was achieved in a short time without any complicated reduction and alkylation procedures.
Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai, P. R. China.
Ying
Y
Zhang
Weiman
W
Cao
Minbo
M
Liu
Shiping
S
Yang
Huixia
H
Wu
Haojie
H
Lu
Pengyuan
P
Yang
D020650:Combinatorial Chemistry Techniques / Q000295:instrumentation / Q000379:methods; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D006868:Hydrolysis; D008954:Models, Biological; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism / Q000493:pharmacokinetics*; D014867:Water / Q000378:metabolism*
D050091:Dendrimers; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D014867:Water; D014357:Trypsin