Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritina.
Non-model organisms represent the majority of life forms in our planet. However, the lack of genetic information hinders us to understand the unique biological phenomena in non-model organisms at the molecular level. In this study, we applied a tandem transcriptome and proteome profiling on a non-model marine fouling organism, Bugula neritina. Using a 454 pyrosequencing platform with the updated titanium reagents, we generated a total of 48M bp transcriptome data consisting of 131 450 high-quality reads. Of these, 122 650 reads (93%) were assembled to produce 6392 contigs with an average length of 538 bases and the remaining 8800 reads were singletons. Of the total 15 192 unigenes, 13 863 ORFs were predicated, of which 6917 were functionally annotated based on gene ontology and eukaryotic orthologous groups. Subsequent proteome analysis identified and quantified 882 proteins from B. neritina. These results would provide fundamental and important information for the subsequent studies of molecular mechanism in larval biology, development, antifouling research. Furthermore, we demonstrated, for the first time, the combined use of two high-throughput technologies as a powerful approach for accelerating the studies of non-model but otherwise important species.
Wang
Hao
H
Department of Biology, Hong Kong University of Science and Technology, Hong Kong SAR, P R China.
Zhang
Huoming
H
Wong
Yue Him
YH
Voolstra
Christian
C
Ravasi
Timothy
T
B Bajic
Vladimir
V
Qian
Pei-Yuan
PY
D000818:Animals; D002013:Bryozoa; D002852:Chromatography, Ion Exchange; D020869:Gene Expression Profiling / Q000379:methods*; D007814:Larva / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods; D015398:Signal Transduction
D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger