A highly effective gene delivery vector--hyperbranched poly(2-(dimethylamino)ethyl methacrylate) from in situ deactivation enhanced ATRP.
A hyperbranched 2-(dimethylamino)ethyl methacrylate (DMAEMA) based polymer has been synthesised by a one-pot in situ deactivation enhanced atom transfer radical polymerisation (DE-ATRP); it exhibits much higher transfection ability than linear poly(DMAEMA) and is comparable to the well known branched poly(ethylene imine) (PEI) and the SuperFect dendrimer but with lower cytotoxicity.
Newland
Ben
B
Network of Excellence for Functional Biomaterials, National University of Ireland Galway, Galway, Ireland.
Tai
Hongyun
H
Zheng
Yu
Y
Velasco
Diego
D
Di Luca
Andrea
A
Howdle
Steven M
SM
Alexander
Cameron
C
Wang
Wenxin
W
Pandit
Abhay
A
D016475:3T3 Cells; D000818:Animals; D050091:Dendrimers / Q000737:chemistry; D007097:Imines / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D009757:Nylons / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011095:Polyethylenes / Q000737:chemistry; D014162:Transfection
D050091:Dendrimers; D007097:Imines; D008689:Methacrylates; D009757:Nylons; D011095:Polyethylenes; C407037:poly(2-(dimethylamino)ethyl methacrylate); C505405:poly(ethylene imine)