Determination of the intrinsic redox potentials of FeS centers of respiratory complex I from experimental titration curves.
Recently, Euro et al. [Biochem. 47, 3185 (2008) ] have reported titration data for seven of nine FeS redox centers of complex I from Escherichiacoli. There is a significant uncertainty in the assignment of the titration data. Four of the titration curves were assigned to N1a, N1b, N6b, and N2 centers; one curve either to N3 or N7; one more either to N4 or N5; and the last one denoted Nx could not be assigned at all. In addition, the assignment of the titration data to the N6b/N6a pair is also uncertain. In this paper, using our calculated interaction energies [Couch et al. BBA 1787, 1266 (2009)], we perform statistical analysis of these data, considering a variety of possible assignments, find the best fit, and determine the intrinsic redox potentials of the centers. The intrinsic potentials could be determined with an uncertainty of less than +/-10 mV at a 95% confidence level for best fit assignments. We also find that the best agreement between theoretical and experimental titration curves is obtained with the N6b-N2 interaction equal to 71+/-14 or 96+/-26 mV depending on the N6b/N6a titration data assignment, which is stronger than was expected and may indicate a close distance of the N2 center to the membrane surface.
Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Russia.
Emile S
ES
Medvedev
Vernon A
VA
Couch
Alexei A
AA
Stuchebrukhov
D003627:Data Interpretation, Statistical; D042967:Electron Transport Complex I / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D010084:Oxidation-Reduction
D007506:Iron-Sulfur Proteins; D042967:Electron Transport Complex I