[Study on molecular characteristics regarding DNA genotype of Mycobacterium tuberculosis clinical strains in Shandong].
OBJECTIVE
To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong.


METHODS
Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units (MIRU) methods.


RESULTS
12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns, and 481 (86.2%) strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level, comparing to the susceptible strains.


CONCLUSIONS
Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.
Shandong Provincial Chest Hospital, Jinan 250013, China. yfdeng@126.com
Yun-feng
YF
Deng
Yan-an
YA
Zhang
Jian-li
JL
Zheng
Hui
H
Jing
Yan
Y
Wang
Hai-ying
HY
Wang
Xin
X
Ma
Zhi-min
ZM
Liu
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014376:Tuberculosis / Q000382:microbiology* / Q000635:transmission; D055815:Young Adult
D004269:DNA, Bacterial