Antioxidant and amine oxidase inhibitory activities of hydroxyurea.
Hydroxyurea (HU, NH(2)CONHOH), or hydroxycarbamide, is a hydroxamic acid derivative used as a drug for anti-neoplasm and sickle-cell disease. In this study, HU was found to have antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals and dose-dependent inhibitory activities against monoamine oxidase (MAO)-A, MAO-B, and semicarbazide-sensitive amine oxidase (SSAO) as compared to controls of clorgyline, deprenyl, and semicarbazide respectively. HU showed mixed-type, competitive-type, and competitive-type inhibition, respectively, with respect to substrates of MAO-A, MAO-B, and SSAO with apparent inhibition constants (Ki) of 19.46, 5.38, and 1.84 microM.
Division of Gastroenterology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
Yuh-Hwa
YH
Liu
Wen-Chun
WC
Wu
Yeh-Lin
YL
Lu
Ying-Jang
YJ
Lai
Wen-Chi
WC
Hou
D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry; D006918:Hydroxyurea / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D050891:Oxidoreductases Acting on CH-NH2 Group Donors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010851:Picrates / Q000737:chemistry
D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D010851:Picrates; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D050891:Oxidoreductases Acting on CH-NH2 Group Donors; D006918:Hydroxyurea