Expression of CYP19 and CYP17 is associated with leg length, weight, and BMI.
This study investigates associations between gene expressions of aromatase (CYP19), 17α hydroxylase (CYP17), and estrogen receptors α and β and anthropometric measurements in offspring of the Michigan fish eater cohort. Leg and trunk length, height, weight, and BMI and gene expression in peripheral blood cells were measured in offspring of the Michigan fish eater cohort. The parental generation was followed between 1973 and 1991, and maternal age, height, and weight data were collected. Female offspring were contacted in 2001/2002 and followed up in 2006/2007; offspring information included age, education, reproductive history, smoking, and exercise. Gene expression was standardized against 18S ribosomal ribonucleic acid (18SrRNA) and RNA polymerase II (RNA PolII) expressions. Mixed models assessed the statistical effect of gene expression on anthropometric outcomes, accounting for multiple offspring from one mother. Anthropometric measurements and gene expression were measured in 139 female offspring. The two length and the height measurements were correlated, as were BMI and weight. CYP19 expression was correlated with the other gene expressions and both estrogen receptor expressions were associated. For every 1 unit of ΔC(t) (18SrRNA - CYP19) or ΔC(t) (RNA PolII - CYP19), BMI was increased by 0.9 (P = 0.03) and 0.87 kg/m(2) (P = 0.04), respectively, and weight by 2.35 kg (P = 0.03) and 2.1 kg (P = 0.03), respectively. For every 1 unit of ΔC(t) (18SrRNA - CYP17), leg length was increased by 0.84 cm (P = 0.04). The results suggest that CYP17 gene expression may influence growth during childhood and adolescence while CYP19 may be associated with the concurrent measures of weight and BMI.
Department of Epidemiology and Biostatistics, University of South Carolina, Arnold School of Public Health, Columbia, South Carolina, USA.
Mitra
M
Yousefi
Wilfried
W
Karmaus
Lanay M
LM
Mudd
Jeffrey R
JR
Landgraf
Dorota
D
Mikucki
Pamela S
PS
Haan
Jessica
J
Zhang
Janet R
JR
Osuch
D000328:Adult; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D001823:Body Composition / Q000235:genetics; D015992:Body Mass Index; D001835:Body Weight / Q000502:physiology*; D015331:Cohort Studies; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D007866:Leg / Q000033:anatomy & histology / Q000502:physiology; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
D011960:Receptors, Estrogen; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase