Ecological temporal stability of Staphylococcus aureus nasal carriage.
We described the colonization dynamics of Staphylococcus aureus in a group of 266 healthy carriers over a period of approximately 1 year. We used precise genotyping methods, i.e., amplified fragment length polymorphism (AFLP), spa typing, and double-locus sequence typing (DLST), to detect changes in strain identity. Strain change took place rather rarely: out of 89 carriers who had initially been colonized, only 7 acquired a strain different from the original one. Approximately one-third of the carriers eliminated the colonization, and a similar number became newly colonized. Some of these events probably represent detection failure rather than genuine colonization loss or acquisition. Lower bacterial counts were associated with increased probability of eliminating the colonization. We have confirmed a high mutation rate in the spa locus: 6 out of 53 strains underwent mutation in the spa locus. There was no overall change in S. aureus genotype composition.
Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland. olga.sakwinska@unil.ch
Olga
O
Sakwinska
Dominique S
DS
Blanc
Catherine
C
Lazor-Blanchet
Martine
M
Moreillon
Marlyse
M
Giddey
Philippe
P
Moreillon
D000328:Adult; D015373:Bacterial Typing Techniques; D044822:Biodiversity; D002353:Carrier State / Q000382:microbiology*; D015169:Colony Count, Microbial; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D009297:Nasal Mucosa / Q000382:microbiology*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D004269:DNA, Bacterial; D013205:Staphylococcal Protein A