T cell-based functional cDNA library screening identified SEC14-like 1a carboxy-terminal domain as a negative regulator of human immunodeficiency virus replication.
Genome-wide screening of host factors that regulate HIV-1 replication has been attempted using numerous experimental approaches. However, there has been limited success using T cell-based cDNA library screening to identify genes that regulate HIV-1 replication. We have established a genetic screening strategy using the human T cell line MT-4 and a replication-competent HIV-1. With this system, we identified the C-terminal domain (CTD) of SEC14-like 1a (SEC14L1a) as a novel inhibitor of HIV-1 replication. Our T cell-based cDNA screening system provides an alternative tool for identifying novel regulators of HIV-1 replication.
National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Emiko
E
Urano
Reiko
R
Ichikawa
Yuko
Y
Morikawa
Takeshi
T
Yoshida
Yoshio
Y
Koyanagi
Jun
J
Komano
D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015723:Gene Library; D005822:Genetic Vectors; D015658:HIV Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D010957:Plasmids; D013601:T-Lymphocytes / Q000276:immunology*; D014779:Virus Replication
D002352:Carrier Proteins; C100642:SEC14L1 protein, human