Characterization of poly(epsilon-caprolactone)/polyfumarate blends as scaffolds for bone tissue engineering.
There is considerable interest in the design of polymeric biomaterials that can be used for the repair of bone defects. In this study, we used ultrasound to prepare a compatibilized blend of poly(epsilon-caprolactone) (PCL) and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a 50% decrease in ultimate tensile stress (P < 0.05) and an 84% decrease in elongation-at-break (P < 0.05). However, the mechanical properties of this blend were comparable to those of trabecular bone. We next evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for comparison, with UMR106 and MC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be useful in future applications for bone regeneration.
Instituto de Investigaciones Fisicoquímica Teóricas y Aplicadas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CC 16 Suc. 4, 1900 La Plata, Argentina.
Juan Manuel
JM
Fernandez
M Silvina
MS
Molinuevo
Ana M
AM
Cortizo
Antonio D
AD
McCarthy
M Susana
MS
Cortizo
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D001842:Bone and Bones; D002448:Cell Adhesion; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005650:Fumarates / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry*; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
D001672:Biocompatible Materials; D005650:Fumarates; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone