db-Cyclic adenosine monophosphate promotes axon regeneration and motor function recovery in cerebral ischemia-reperfusion rats.
BACKGROUND
There is no effective axon regeneration in adult mammalians.


OBJECTIVE
To investigate the effects of dual-acid cyclic adenosine monophosphate (db-cAMP) on the axon regeneration, motor function recovery and RhoA signal pathway in cerebral ischemia-reperfusion rats, and to explore the possible clinical application and mechanism.


METHODS
Middle cerebral artery ischemia-reperfusion model was established by nylon monofilament occlusion method in 105 Sprague-Dawley (SD) rats. Semi-quantitative Western blot analysis was used to assess protein expression level of growth-associated protein-43 (GAP-43) and RhoA. Montoya staircase test score was used to test the motor function of affected forelimb.


RESULTS
Compared to the ischemia group, the staircase test score in the db-cAMP group was increased significantly at 30-day (P < 0.05), and GAP-43 protein expression in the db-cAMP group was enhanced significantly at 7-day and 14-day (P < 0.05), and RhoA protein expression in the db-cAMP group was decreased significantly between 24 h to 14-day (P < 0.01).


CONCLUSIONS
These results show that db-cAMP can promote axon regeneration and the recovery of motor function by inhibiting RhoA signal pathway.
Niu
Lingchuan
L
Department of Neurology, The Second Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhou
Jia
J
Huang
Ying
Y
Chen
Yan
Y
Li
Changqing
C
D000818:Animals; D001369:Axons / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology* / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005552:Forelimb / Q000503:physiopathology; D019922:GAP-43 Protein / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D011597:Psychomotor Performance / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D015424:Reperfusion; D013997:Time Factors; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D019922:GAP-43 Protein; D000242:Cyclic AMP; D020741:rho GTP-Binding Proteins