Up-regulation of Idh3alpha causes reduction of neuronal differentiation in PC12 cells.
The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate Idh3alpha expression by the time course. To identify the function of Idh3alpha in neuronal differentiation stage, the transfection of Idh3alpha to PC12 cells was performed. As a result, we proved that up-regulation of Idh3alpha causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that Idh3alpha plays a role to the neuronal differentiation.
Cho
Sun A
SA
Department of Biological Science, Sookmyung Womenos University, Seoul, Korea.
Seo
Min-Ji
MJ
Ko
Je Yeong
JY
Shim
Jung-Hee
JH
Yoo
Jin
J
Kim
Jung-Hee
JH
Kim
Se Yoon
SY
Ryu
Na Kyung
NK
Park
Eun Young
EY
Lee
Han-Woong
HW
Lee
Yeon-Su
YS
Bahk
Young Yil
YY
Park
Jong-Hoon
JH
D000818:Animals; D002454:Cell Differentiation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D016716:PC12 Cells / Q000502:physiology*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
D007527:Isoenzymes; D020543:Proteome; D007521:Isocitrate Dehydrogenase; D048049:Extracellular Signal-Regulated MAP Kinases