[Identification of the active material of anti-hepatic fibrosis from Amydae Carapax].
OBJECTIVE
To identify the active material of anti-hepatic fibrosis from Amydae Carapax.


METHODS
Membrane separation technology was adopted to screen active fraction in Amydae Carapax, and the active components were isolated from the active fraction using gel chromatography and high performance liquid chromatography. The purified active components in Amydae Carapax were further analyzed using 4700 series time-of-flight mass spectrometer.


RESULTS
Proteins and peptides of Amydae Carapax with molecular weight less than 6000 were proved to have biological activity. 8 components (Bj1-Bj8) were isolated from the active fraction. Bj4, Bj6 and Bj7 were screened as active components. Bj7 was further purified, resulting in 7 components (Bj701-Bj707). Bj704 and Bj707 showed significant biological activity. Mass spectrometry showed three molecular ion peaks with highest abundance, i.e. m/e 526, 542 and 572, i.e. m/e 526, 542 and 572, in Bj707 -A The amino acid sequences of above three peptide compounds were NDDY (Asn-Asp-Asp-Tyr), NPNPT (Asn-Pro-Asn-Pro-Thr), and HGRFG (His-Gly-Arg-Phe-Gly), respectively. And M572 was the most abandunt components.


CONCLUSIONS
Three active peptide compounds of anti-hepatic fibrosis of Amydae Carapax were identified.
Department of Infectious Diseases, Zhejiang Quhua Hospital ,Quzhou Zhejiang Province 324004, China. gjr.gao@gmail.com
Jian-rong
JR
Gao
Yan-wen
YW
Liu
Chang-yu
CY
Li
Hang-ping
HP
Yao
Chi-zhi
CZ
Zhang
Jin-wen
JW
Chen
Zhi-hua
ZH
Shao
Jian-wen
JW
Liu
Wei-min
WM
Cai
Yong-xiang
YX
Ding
You-fa
YF
Zhu
Yin-ping
YP
Tang
Chun-ling
CL
Hu
Jing-ni
JN
Shi
Zu-liang
ZL
Hu
Hong-qiu
HQ
Zhang
Ling
L
Yang
D000818:Animals; D002460:Cell Line; D006801:Humans; D008103:Liver Cirrhosis; D008516:Medicine, Chinese Traditional; D014020:Tissue Extracts / Q000302:isolation & purification* / Q000494:pharmacology*
D014020:Tissue Extracts