Stereotyped patterns of B-cell receptor in splenic marginal zone lymphoma.
Antigen stimulation may be important for splenic marginal zone lymphoma pathogenesis. To address this hypothesis, the occurrence of stereotyped B-cell receptors was investigated in 133 SMZL (26 HCV+) compared with 4,414 HCDR3 sequences from public databases. Sixteen SMZL (12%) showed stereotyped BCR; 7 of 86 (8%) SMZL sequences retrieved from public databases also belonged to stereotyped HCDR3 subsets. Three categories of subsets were identified: i) "SMZL-specific subsets" (n=5), composed only of 12 SMZL (9 HCV-from our series); ii) "Non-Hodgkin's lymphoma-like subsets" (n=5), comprising 5 SMZL (4 from our series) clustering with other indolent lymphomas; iii) "CLL-like subsets" (n=6), comprising 6 SMZL (3 from our series) that belonged to known CLL subsets (n=4) or clustered with public CLL sequences. Immunoglobulin 3D modeling of 3 subsets revealed similarities in antigen binding regions not limited to HCDR3. Overall, data suggest that the pathogenesis of splenic marginal zone lymphoma may involve also HCV-unrelated epitopes or an antigenic trigger common to other indolent lymphomas.
Division of Hematology, Department of Oncohematology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Viale Golgi 19, Pavia, Italy.
Silvia
S
Zibellini
Daniela
D
Capello
Francesco
F
Forconi
Paolo
P
Marcatili
Davide
D
Rossi
Sara
S
Rattotti
Silvia
S
Franceschetti
Elisa
E
Sozzi
Emanuele
E
Cencini
Roberto
R
Marasca
Luca
L
Baldini
Alessandra
A
Tucci
Francesco
F
Bertoni
Francesco
F
Passamonti
Ester
E
Orlandi
Marzia
M
Varettoni
Michele
M
Merli
Silvia
S
Rizzi
Valter
V
Gattei
Anna
A
Tramontano
Marco
M
Paulli
Gianluca
G
Gaidano
Luca
L
Arcaini
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens; D000939:Epitopes; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D018442:Lymphoma, B-Cell, Marginal Zone / Q000209:etiology* / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013160:Splenic Neoplasms / Q000209:etiology* / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D011947:Receptors, Antigen, B-Cell