New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA.
Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p orthologue from the crenarchaeon Sulfolobus acidocaldarius forms 1-methyladenosine at the same position. Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N(1)-atom of either adenosine or guanosine at position 9 in different tRNAs. This is to our knowledge the first example of a tRNA methyltransferase with a broadened nucleoside recognition capability. The evolution of tRNA methyltransferases methylating the N(1) atom of a purine residue is discussed.
Laboratoire de Microbiologie, Université Libre de Bruxelles, Institut de Recherches Microbiologiques Jean-Marie Wiame, Avenue E Gryson 1, B-1070 Bruxelles, Belgium.
Morgane
M
Kempenaers
Martine
M
Roovers
Yamina
Y
Oudjama
Karolina L
KL
Tkaczuk
Janusz M
JM
Bujnicki
Louis
L
Droogmans
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D019843:Archaeal Proteins / Q000145:classification / Q000378:metabolism*; D055162:Biocatalysis; D004926:Escherichia coli / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism; D016945:Sulfolobus acidocaldarius / Q000201:enzymology; D019712:Thermococcus / Q000201:enzymology; D012359:tRNA Methyltransferases / Q000145:classification / Q000378:metabolism*
D019843:Archaeal Proteins; D006151:Guanosine; C002230:1-methyladenosine; C020770:1-methylguanosine; D012343:RNA, Transfer; D012359:tRNA Methyltransferases; C023194:tRNA (adenine(58)-N(1))-methyltransferase; C036950:tRNA (guanine-N1-)-methyltransferase; D000241:Adenosine