Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.
Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.
Chigu
Nomathemba Loice
NL
Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Hirosue
Sinji
S
Nakamura
Chie
C
Teramoto
Hiroshi
H
Ichinose
Hirofumi
H
Wariishi
Hiroyuki
H
D000873:Anthracenes / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D020869:Gene Expression Profiling; D053858:Metabolic Networks and Pathways; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D020075:Phanerochaete / Q000201:enzymology* / Q000378:metabolism*; D000095322:Anthrones
D000873:Anthracenes; D000880:Anthraquinones; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C034020:anthracene; C004522:anthrone; D000095322:Anthrones