Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy.
We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.
Hashimoto
Hiroshi
H
RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Isobe
Keisuke
K
Suda
Akira
A
Kannari
Fumihiko
F
Kawano
Hiroyuki
H
Mizuno
Hideaki
H
Miyawaki
Atsushi
A
Midorikawa
Katsumi
K
D001426:Bacterial Proteins / Q000737:chemistry; D005583:Fourier Analysis; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007834:Lasers; D008164:Luminescent Proteins / Q000737:chemistry*; D017711:Nonlinear Dynamics; D055620:Optical Phenomena; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data
D001426:Bacterial Proteins; D008164:Luminescent Proteins; C527701:enhanced cyan fluorescent protein; C485184:enhanced green fluorescent protein; C413663:fluorescent protein 583; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins