Seventy-two-hour preservation, resuscitation, and transplantation of an isolated rat heart with high partial pressure carbon monoxide gas (PCO = 400 hPa) and high partial pressure carbon dioxide (PCO(2) = 100 hPa).
The cardiac cavity of an isolated rat heart was filled with a Krebs-Henseleit (KH) solution, and the heart was hung in a high-pressure chamber. After the high-pressure chamber had been filled with a mixed gas (PCO = 400 hPa, PCO(2) = 100 hPa, PO(2) = 900 hPa, PHe = 5600 hPa) and preserved for 72 h, we performed a cervical ectopic heart transplantation on a recipient rat and resuscitated the preserved heart. This is the first incidence in the world of a mammalian organ having been successfully preserved and resuscitated after 72 h via a desiccation method.
Hatayama
Naoyuki
N
Resonance Club Co, Ltd, Tokyo, Japan. nao_h416@yahoo.co.jp
Yoshida
Yu
Y
Seki
Kunihiro
K
D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D002248:Carbon Monoxide / Q000494:pharmacology*; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects*; D016027:Heart Transplantation / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D010313:Partial Pressure; D051381:Rats; D012151:Resuscitation / Q000379:methods*; D013997:Time Factors
D002245:Carbon Dioxide; D002248:Carbon Monoxide