A deuterohemin peptide extends lifespan and increases stress resistance in Caenorhabditis elegans.
This group has invented a novel deuterohemin containing peptide deuterohemin-AlaHisThrValGluLys (DhHP-6), which has various biological activities including protection of murine ischemia reperfusion injury, improving cell survival and preventing apoptosis. It was hypothesized that DhHP-6 is beneficial on the lifespan of Caenorhabditis elegans (C. elegans) and increases their resistance to heat and oxidative stress. C. elegans were treated with different concentrations of DhHP-6. Survival time and sensitivity to heat and paraquat were investigated. The data demonstrated that the mean survival time of C. elegans was significantly increased (p < 0.05) in the DhHP-6 treated group compared with the control group. The maximum lifespan was not affected by DhHP-6 treatment. DhHP-6 improved the survival rate of C. elegans in the acute heat stress (35 degrees C) and rescued the C. elegans' sensitivity to paraquat in acute oxidative stress. Superoxide dismutase 3 (SOD-3) protein was up-regulated by DhHP-6 treatment. It was further demonstrated that stress resistance genes such as hsp-16.1, hsp-16.49 and sir-2.1 were regulated by DhHP-6. DAF-16 and SIR-2.1 genes are essential for the beneficial effect of DhHP-6. Therefore, the investigation into the beneficial effect of DhHP-6 on C. elegans' lifespan has the potential to develop novel drugs to prevent ageing.
Jilin University, Changchun, P R China.
Shuwen
S
Guan
Pengfei
P
Li
Jing
J
Luo
Yuanyuan
Y
Li
Lei
L
Huang
Guan
G
Wang
Limin
L
Zhu
Hongkuan
H
Fan
Wei
W
Li
Liping
L
Wang
D000375:Aging / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D045303:Cytochromes b; D004353:Drug Evaluation, Preclinical; D051858:Forkhead Transcription Factors; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D017238:Genes, Helminth; D006427:Hemin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006358:Hot Temperature; D008136:Longevity / Q000187:drug effects / Q000235:genetics; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000633:toxicity; D037761:Sirtuins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological / Q000187:drug effects*; D013385:Succinate Dehydrogenase / Q000172:deficiency / Q000502:physiology; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; C472666:MEV-1 protein, C elegans; D009842:Oligopeptides; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; C552681:deuterohemin-alanyl-histidyl-threonyl-valyl-glutamyl-lysine; D006427:Hemin; D045303:Cytochromes b; D013385:Succinate Dehydrogenase; C507014:SIR-2.1 protein, C elegans; D037761:Sirtuins; D010269:Paraquat