Studies on expression of FSH and its anti-apoptotic effects on ischemia injury in rat spinal cord.
Studies indicated that many tissues could express FSH. New functions of FSH have been recognized beyond reproduction regulation. However, no report has been made about the expression and function of FSH in rat spinal cord. Double-labeled immunofluorescence stain and in situ hybridization were used to study the co-localization of FSH with its receptor and co-localization of FSH with GnRH receptor in rat spinal cord. Spinal cord ischemia injury models were built, TUNEL stain and Fas immunostaining were made to observe the anti-apoptotic effects of FSH to neurons induced by spinal cord ischemia injury. The results found that some neurons and glias of rat spinal cord showed both FSH immunoreactivity and FSH mRNA positive signals; not only FSH and its receptor but also FSH and GnRH receptor co-located in cells of both gray matter and white matter; treatment with certain concentration of FSH before ischemia-reperfusion injury, less TUNEL positive cells and Fas positive cells were found in motor neurons of ventral gray matter in FSH experiment group than that in control group. These suggested that rat spinal cord could express FSH, it is also a target organ of FSH; FSH might exert functions through its receptor by paracrine or autocrine effects; GnRH in spinal cord might regulate FSH positive neurons through GnRH receptor; FSH might inhibit ischemia induced neuron apoptosis by down-regulating Fas expression in spinal cord.
Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China. chuchenyu1977@yahoo.com.cn
Chenyu
C
Chu
Bainan
B
Xu
Weiquan
W
Huang
D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis* / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis; D020287:In Situ Nick-End Labeling; D008297:Male; D009046:Motor Neurons / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011962:Receptors, FSH / Q000096:biosynthesis; D011966:Receptors, LHRH / Q000096:biosynthesis; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D013116:Spinal Cord / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D019014:fas Receptor / Q000096:biosynthesis
C491898:Fas protein, rat; D011962:Receptors, FSH; D011966:Receptors, LHRH; D019014:fas Receptor; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone