Food processing increases casein resistance to simulated infant digestion.
The objective of this study was to determine whether processing could modify the resistance of casein (CN) to digestion in infants. A range of different dairy matrices was manufactured from raw milk in a pilot plant and subjected to in vitro digestion using an infant gut model. Digestion products were identified using MS and immunochemical techniques. Results obtained showed that CNs were able to resist digestion, particularly κ- and αs(2)-CN. Resistant areas were identified and corresponded to fragments hydrophobic at pH 3.0 (gastric conditions) and/or carrying post-translational modifications (phosphorylation and glycosylation). Milk processing led to differences in peptide patterns and heat treatment of milk tended to increase the number of peptides found in digested samples. This highlights the likely impact of milk processing on the allergenic potential of CNs.
Dupont
Didier
D
INRA, UMR1253 Science et Technologie du Lait et de l'Œuf,Rennes, France. didier.dupont@rennes.inra.fr
Mandalari
Giuseppina
G
Mollé
Daniel
D
Jardin
Julien
J
Rolet-Répécaud
Odile
O
Duboz
Gabriel
G
Léonil
Joëlle
J
Mills
Clare E N
CE
Mackie
Alan R
AR
D000595:Amino Acid Sequence; D002364:Caseins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004063:Digestion; D005511:Food Handling / Q000379:methods*; D005512:Food Hypersensitivity / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007223:Infant; D048668:Lactobacillus delbrueckii / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics; D011499:Protein Processing, Post-Translational; D048249:Streptococcus thermophilus / Q000378:metabolism; D015014:Yogurt / Q000032:analysis
D002364:Caseins; D010455:Peptides