Rapid spectrally encoded fluorescence imaging using a wavelength-swept source.
We present rapid imaging of fluorescent samples using spectral encoding (SE). A near-IR wavelength-swept source in used to preserve the SE of the position, despite Stokes shifts. To validate this approach, we imaged fluorescent PbS quantum dot solutions at concentrations down to 0.5+/-0.1micromol/L. This simple configuration allowed acquisition rates of up to 9920 lines of 1024 pixels per second to create high-resolution images. This spectrally encoded setup could be easily miniaturized for endoscopy, thus combining high-resolution fluorescence with confocal reflectance imaging at unmatched acquisition speed.
Engineering Physics Department, Ecole Polytechnique Montréal,2900, Boulevard Edouard-Montpetit, Montreal, Quebec, H3C3A7, Canada.
Mathias
M
Strupler
Etienne De
ED
Montigny
Dominic
D
Morneau
Caroline
C
Boudoux
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D016247:Information Storage and Retrieval / Q000379:methods*; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity