Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy.
We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.
RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Hiroshi
H
Hashimoto
Keisuke
K
Isobe
Akira
A
Suda
Fumihiko
F
Kannari
Hiroyuki
H
Kawano
Hideaki
H
Mizuno
Atsushi
A
Miyawaki
Katsumi
K
Midorikawa
D001426:Bacterial Proteins / Q000737:chemistry; D005583:Fourier Analysis; D049452:Green Fluorescent Proteins / Q000737:chemistry; D007834:Lasers; D008164:Luminescent Proteins / Q000737:chemistry*; D017711:Nonlinear Dynamics; D055620:Optical Phenomena; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data
D001426:Bacterial Proteins; D008164:Luminescent Proteins; C527701:enhanced cyan fluorescent protein; C485184:enhanced green fluorescent protein; C413663:fluorescent protein 583; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins