Immunofluorescence and immunoperoxidase screening of hybridomas.
As mentioned in the previous chapter, the provision of a rapid, simple assay for screening monoclonal antibodies is essential for their production. Although solid-phase binding assays are the method of choice ( Chapter 30 ) a number of applications require visualization of antibody binding to cultured cells or tissue sections.
Wood
J N
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Department of Experimental lmmunoblology, Wellcome Research Laboratories, Beckenham, Kent.