Cistaceae aqueous extracts containing ellagitannins show antioxidant and antimicrobial capacity, and cytotoxic activity against human cancer cells.
Roots and aerial parts of Cistaceae have been used since ancient times in the Mediterranean cultures for its medicinal properties. In this study, phenolic and tannin content of C. ladanifer and C. populifolius leaves aqueous extracts were determined and their antioxidant and antimicrobial activity were fully studied by several in vitro assays. Their major compounds were identified and quantitated by high-performance liquid chromatography with diode array detection coupled to electrospray ion-trap mass spectrometry. Cytotoxicity on a panel of human cancer cells was also determined. C. populifolius extract was stronger antioxidant than C. ladanifer extract in electron transfer reaction based assays but C. ladanifer extract was more effective to inhibit peroxyl radicals. The major compounds in both extracts were ellagitannins, especially punicalagins derivatives, showing C. populifolius a higher content. C. ladanifer showed noteworthy antibacterial activity against Staphylococcus aureus, whereas C. populifolius was effective against Escherichia coli, with MICs values of 154 and 123 microg/mL, respectively. Last, both extracts showed a notorious capacity to inhibit the proliferation of M220 pancreatic cancer cells and MCF7/HER2 and JIMT-1 breast cancer cells. The leaves of these plants suppose a source for water-soluble ellagitannins-enriched polyphenolic extracts with antioxidant and antimicrobial activities. Their cytotoxic activity against several cancer cells may deserve further attention.
Molecular and Cellular Biology Institute, Miguel Hernández University, E-03202 Elche, Alicante, Spain.
Enrique
E
Barrajón-Catalán
Salvador
S
Fernández-Arroyo
Domingo
D
Saura
Emilio
E
Guillén
Alberto
A
Fernández-Gutiérrez
Antonio
A
Segura-Carretero
Vicente
V
Micol
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002839:Chromans / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D030601:Cistaceae / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D047348:Hydrolyzable Tannins / Q000737:chemistry* / Q000494:pharmacology*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D013634:Tannins / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002839:Chromans; D005290:Ferric Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid