Cutaneous challenge with chemical warfare agents in the SKH-1 hairless mouse (II): effects of some currently used skin decontaminants (RSDL and Fuller's earth) against liquid sulphur mustard and VX exposure.
Using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: Fuller's earth (FE) and Reactive Skin Decontamination Lotion (RSDL) against two extremely toxic chemical warfare agents that represent a special percutaneous hazard, sulphur mustard (SM) and O-ethyl-S-(2[di-isopropylamino]ethyl)methyl-phosphonothioate (VX). Five minutes after being exposed on the back to either 2 µL of neat sulphur mustard or 50 µg.kg(-1) of diluted VX, mice were decontaminated. Both systems were able to reduce blisters 3 days after SM exposure. However, RSDL was found to be more efficient than FE in reducing the necrosis of the epidermis and erosion. In the case of VX exposure, RSDL, whatever the ratio of decontaminant to toxicant used (RSDL 10, 20, 50), was not able to sufficiently prevent the inhibition of plasma cholinesterases taken as a surrogate marker of exposure and toxicity. Only FE reduced significantly the ChE inhibition. Some of these observations are different from our previous results obtained in domestic swine and these changes are thus discussed in the perspective of using SKH-1 hairless mice for the initial in vivo screening of decontaminants.
Taysse
L
L
Centre d'études du Bouchet (Defense research center) Vert-Le-Petit, France. laurent.taysse@dga.defense.gouv.fr
Dorandeu
F
F
Daulon
S
S
Foquin
A
A
Perrier
N
N
Lallement
G
G
Breton
P
P
D017607:Aluminum Compounds / Q000008:administration & dosage*; D000818:Animals; D002619:Chemical Warfare Agents / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage; D003666:Decontamination; D003931:Diacetyl / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004195:Disease Models, Animal; D017616:Magnesium Compounds / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D009151:Mustard Gas / Q000633:toxicity*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D017640:Silicates / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects / Q000473:pathology
D017607:Aluminum Compounds; D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D003931:Diacetyl; D017616:Magnesium Compounds; D009151:Mustard Gas; D009946:Organothiophosphorus Compounds; D011092:Polyethylene Glycols; D017640:Silicates; C004717:diacetylmonoxime; C001262:Fuller's Earth; C028210:monomethoxypolyethylene glycol; C009680:VX-agent