Survival and function of CD47-deficient thymic grafts in mice.
BACKGROUND
We have previously shown that the interspecies incompatibility of CD47 plays an important role in triggering rejection of xenogeneic hematopoietic cells by macrophages. However, it remains unknown whether CD47 incompatibility also contributes to the rejection of non-hematopoietic xenografts.


OBJECTIVE
Here, we investigated the role of CD47 in preventing macrophage-mediated rejection of thymic epithelial cells in a mouse model of thymic transplantation across the CD47 barrier.


METHODS
Wild-type (WT) and CD47 KO mice were thymectomized and treated with T cell-depleting mAbs, and implanted with fetal thymus from syngeneic WT or CD47 KO donors.


RESULTS
Transplantation of CD47 KO mouse thymus led to T cell recovery in thymectomized, T cell-depleted WT mice. Similar to the control WT mouse thymic grafts, CD47 KO mouse thymic grafts showed a normal distribution of thymocyte subsets, and almost all of the thymocytes were recipient origin. Furthermore, histological analysis confirmed long-term survival of CD47 KO mouse thymic epithelial cells in WT mouse recipients.


CONCLUSIONS
These results demonstrate that, unlike hematopoietic cells, CD47 KO mouse thymus can survive and function in WT mice. Furthermore, our data implicate that the role of CD47 in xenograft rejection may differ for different types of xenografts, and that CD47 incompatibility is unlikely to impede thymic xenotransplantation, a potential approach to inducing xenotolerance, by triggering macrophage-mediated rejection.
Wang
Yuantao
Y
First Hospital of Jilin University, Changchun, Jilin, China.
Wang
Hui
H
Wang
Shumei
S
Fu
Yaowen
Y
Yang
Yong-Guang
YG
D000818:Animals; D051928:CD47 Antigen / Q000276:immunology*; D004847:Epithelial Cells / Q000166:cytology / Q000637:transplantation; D006085:Graft Survival / Q000276:immunology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000276:immunology*
D051928:CD47 Antigen