Neonatal total liquid ventilation: is low-frequency forced oscillation technique suitable for respiratory mechanics assessment?
This study aimed to implement low-frequency forced oscillation technique (LFFOT) in neonatal total liquid ventilation (TLV) and to provide the first insight into respiratory impedance under this new modality of ventilation. Thirteen newborn lambs, weighing 2.5 + or - 0.4 kg (mean + or - SD), were premedicated, intubated, anesthetized, and then placed under TLV using a specially design liquid ventilator and a perfluorocarbon. The respiratory mechanics measurements protocol was started immediately after TLV initiation. Three blocks of measurements were first performed: one during initial respiratory system adaptation to TLV, followed by two other series during steady-state conditions. Lambs were then divided into two groups before undergoing another three blocks of measurements: the first group received a 10-min intravenous infusion of salbutamol (1.5 microg x kg(-1) x min(-1)) after continuous infusion of methacholine (9 microg x kg(-1) x min(-1)), while the second group of lambs was chest strapped. Respiratory impedance was measured using serial single-frequency tests at frequencies ranging between 0.05 and 2 Hz and then fitted with a constant-phase model. Harmonic test signals of 0.2 Hz were also launched every 10 min throughout the measurement protocol. Airway resistance and inertance were starkly increased in TLV compared with gas ventilation, with a resonant frequency < or = 1.2 Hz. Resistance of 0.2 Hz and reactance were sensitive to bronchoconstriction and dilation, as well as during compliance reduction. We report successful implementation of LFFOT to neonatal TLV and present the first insight into respiratory impedance under this new modality of ventilation. We show that LFFOT is an effective tool to track respiratory mechanics under TLV.
Faculté de Médecine et des Sciences de la Santé, Département de Pédiatrie, 3001, 12e Ave. Nord, Sherbrooke, Québec, Canada J1H 5N4.
Dominick
D
Bossé
Alexandre
A
Beaulieu
Olivier
O
Avoine
Philippe
P
Micheau
Jean-Paul
JP
Praud
Hervé
H
Walti
D000403:Airway Resistance; D000420:Albuterol / Q000008:administration & dosage; D000818:Animals; D000831:Animals, Newborn; D016084:Bronchoconstriction; D016085:Bronchoconstrictor Agents / Q000008:administration & dosage; D001993:Bronchodilator Agents / Q000008:administration & dosage; D007262:Infusions, Intravenous; D021061:Liquid Ventilation / Q000379:methods*; D008170:Lung Compliance; D016210:Methacholine Chloride / Q000008:administration & dosage; D009991:Oscillometry; D012129:Respiratory Function Tests / Q000379:methods*; D015656:Respiratory Mechanics / Q000187:drug effects; D012756:Sheep
D016085:Bronchoconstrictor Agents; D001993:Bronchodilator Agents; D016210:Methacholine Chloride; D000420:Albuterol