{2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-D-Glucose} suppresses proliferation and induces apoptosis in the human esophageal cancer cell line.
The aim of this study was to investigate the molecular mechanisms of apoptosis induced by {2-[(3-Carboxy-1-oxopropyl) amino]-2-deoxy-D-Glucose} (COPADG) in the esophageal cancer cell line Eca-109 and to establish a relationship between the rate of apoptosis and Fas and Bcl-2 protein expression. Eca-109 cells were cultured under standard condition. Cell growth was measured by MTT assay. Apoptosis and cell proliferation were determined by flow cytometry. Expressions of apoptosis-regulated genes Fas and Bcl-2 were detected by immunohistochemical methods and image analysis. COPADG dose- and time-dependently inhibited the growth of Eca-109 cells in vitro. Incubation of Eca-109 cells with 40 μmol/l of COPADG for 48 h induced significant apoptosis. After drug treatment, Fas protein expression was increased, while Bcl-2 protein expression was decreased. COPADG is able to induce apoptosis in the esophageal cancer cell line Eca-109. The mechanism of apoptosis in these cells may be related to up-regulation of Fas gene expression and the down-regulation of Bcl-2, which may serve as the experimental evidence for development of new drugs for the non-surgical management of human esophageal cancer.
Wu
Jing
J
Department of Gastroenterology and Hepatology, Beijing Shijitan Hospital, Beijing, 100038, China. wu_jing2010@hotmail.com
Lu
Hong
H
Ling
Xiangchun
X
Wang
Canghai
C
Rui
Ji
J
Wang
Aiqin
A
Qiao
Liang
L
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002469:Cell Separation; D003847:Deoxyglucose / Q000494:pharmacology*; D004938:Esophageal Neoplasms / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000096:biosynthesis; D005434:Flow Cytometry; D005944:Glucosamine / Q000031:analogs & derivatives*; D006801:Humans; D007150:Immunohistochemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis
D000970:Antineoplastic Agents; D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D003847:Deoxyglucose; D005944:Glucosamine