Time-lapse imaging of embryonic neural stem cell division in Drosophila by two-photon microscopy.
This unit describes a protocol for live imaging of Drosophila embryonic neural stem cells using two-photon microscopy. Compared to traditional one-photon confocal imaging, this technique renders higher-resolution optical sections from deeper within the embryo. It is ideally suited to following embryonic neuroblasts located underneath the neuroepithelial cell layer for several rounds of cell division.
Cell Division Group, IRB-Barcelona, Barcelona, Spain.
Elena
E
Rebollo
Cayetano
C
Gonzalez
D000818:Animals; D002455:Cell Division; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008297:Male; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000166:cytology*; D017785:Photons; D013997:Time Factors