Metabolomic analysis of thermally injured and/or septic rats.
BACKGROUND
Early diagnosis and treatment for thermal injury with septic complications continue to be a serious clinical problem. In this study, plasma biomarkers of rats in the burn and/or septic models were investigated with a metabolomic method.


METHODS
Rat plasma samples were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Multivariate analysis, the principal components analysis (PCA), was used to validate metabolic changes. In addition, another multivariate method, the orthogonal partial least-squares analysis (OPLS), was used to profile potential biomarkers in models.


RESULTS
Nine characteristic metabolites, including hypoxanthine, indoxyl sufate, glucuronic acid, gluconic acid, proline, uracil, nitrotyrosine, uric acid, and trihydroxy cholanoic acid were identified in models of thermal injury and/or sepsis.


CONCLUSIONS
These biomarkers were mainly involved in oxidative stress and tissue damage, and might supply evidence for distinguishing burned septic patients from non-septic ones.
Liu
Xin-Ru
XR
Department of Medicinal Chemistry of Nature Product, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Zheng
Xing-Feng
XF
Ji
Shi-Zhao
SZ
Lv
Yong-Hai
YH
Zheng
De-Yi
DY
Xia
Zhao-Fan
ZF
Zhang
Wei-Dong
WD
D000596:Amino Acids / Q000097:blood; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002056:Burns / Q000097:blood*; D002264:Carboxylic Acids / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004195:Disease Models, Animal; D006571:Heterocyclic Compounds / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis
D000596:Amino Acids; D015415:Biomarkers; D002264:Carboxylic Acids; D006571:Heterocyclic Compounds