The electrophoretic elution of proteins from polyacrylamide gels.
The analytical power of acrylamide gel electrophoresis is one of the keys of modern protein chemistry. It is not surprising, therefore, that many methods have been described for converting that analytical power into a preparative tool. None of the available methods is entirely satisfactory for general use since loss of resolution or low recovery is often involved. The method described here has given both high resolution and good recovery but suffers from the disadvantage of being relatively laborious (1,2). In addition, although the recovered proteins are good for peptide analysis or amino acid composition determination, we have found very low yields on Edman degradation of proteins eluted from gels (3).
Department of Biological Chemistry, University of California School of Medicine, Davis, California.
J H
JH
Waterborg
H R
HR
Matthews