In vitro secondary caries inhibition by adhesive systems in enamel around composite restorations.
This study compared the ability of adhesive systems to inhibit in vitro caries lesions in enamel under high cariogenic challenge. Bovine enamel blocks with cavity preparations were restored with AP-X resin composite (Kuraray Med) using four adhesives systems: Clearfil SE Bond (Kuraray Med); Clearfil Protect Bond (Kuraray Med); One-Up Bond F (Tokuyama) and Single Bond (3M ESPE). The specimens were submitted to an eight-day pH-cycling regimen. After cariogenic challenge, the enamel was evaluated to detect caries lesions using cross-sectional microhardness, polarized light microscopy and scanning electronic microscopy. Data from cross-sectional microhardness and polarized light microscopy evaluations were statistically analyzed by ANOVA and Tukey's test. The mineral % volume showed no statistical difference among adhesives (p > 0.05); however, polarized light microscopy analysis showed lower caries lesions with Clearfil Protect Bond (p < 0.05). The scanning electron microscopy images showed greater caries lesions and demineralization areas close to restorations for Clearfil SE Bond, One-Up Bond F and Single Bond compared to Clearfil Protect Bond. The pH-cycling regimen promoted subsurface enamel demineralization in all specimens treated. The polarized light microscopy and scanning electronic microscopy analyses showed that Clearfil Protect Bond seems to produce lower enamel demineralization around restorations; however, cross-sectional microhardness did not demonstrate differences among the adhesives.
University of Campinas, Department of Restorative Dentistry, Piracicaba, SP, Brazil.
Cristiane Franco
CF
Pinto
Adriana Franco
AF
Paes-Leme
Gláucia Maria Bovi
GM
Ambrosano
Marcelo
M
Giannini
D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002326:Cariogenic Agents / Q000009:adverse effects; D002327:Cariostatic Agents / Q000737:chemistry*; D002417:Cattle; D003188:Composite Resins / Q000737:chemistry*; D003731:Dental Caries / Q000473:pathology / Q000517:prevention & control*; D003737:Dental Cavity Preparation / Q000379:methods; D003738:Dental Cements / Q000737:chemistry*; D003743:Dental Enamel / Q000187:drug effects / Q000648:ultrastructure*; D003793:Dental Restoration, Permanent / Q000379:methods; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008859:Microscopy, Polarization; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry; D013499:Surface Properties; D017001:Tooth Demineralization / Q000473:pathology / Q000517:prevention & control; D014089:Tooth Remineralization
D002326:Cariogenic Agents; D002327:Cariostatic Agents; C503075:Clearfil Protect Bond; C438313:Clearfil SE Bond; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C446260:One-Up-Bond F; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate