[The significance of hematopoietic cell genetic instability in aplastic anemia.].
OBJECTIVE
To evaluate bone marrow hematopoietic cells genetic instability (BMHCGI) in patients with aplastic anemia (AA) and to explore its influence on immunosupressive therapy for AA and significance on late clonal hematologic disorders.


METHODS
Genetic instability of bone marrow mononuclear cells (BMMNC) was measured by Comet assay. The relationship between bone marrow failure parameters and genetic instability results was evaluated. The reciprocity of genetic instability and treatment responses to immunosuppressive therapy (IST) was investigated.


RESULTS
Comet assay parameters \[tail moment (TM), olive TM (OTM), comet %\] of AA patients were significantly higher than that of control group (P < 0.05). There was no statistic correlation of comet parameters of severe AA (SAA) BM hematopoietic cells with age, gender and peripheral blood cell count (P > 0.05). For the treatment response rate at six months after IST there was no statistical difference between comet cells of < 21.64% and of >/= 21.64%, and so did between OTM < 1.58 and >/= 1.58 in SAA patients. IST had no effect on SAA BMHCGI, whereas, the Comet%, TM and OTM in SAA PR patients and Comet% in CR patients were significantly decreased than those before treatment. Comet parameters of two SAA patients were significantly increased before the development of clonal cytogenetic abnormalities.


CONCLUSIONS
Increased BMHCGI may be one of the elements in the pathogenetic mechanisms in AA. The genetic instability is irrelevant to the SAA patients overall response rate of IST at six months, but IST can alleviate the genetic instabilities in responded SAA patients.
Zhang
Li-Hong
LH
Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.
Wang
Hui-Jun
HJ
Zhang
Li
L
Zhou
Kang
K
Yang
Dong-Lin
DL
Yan
Zhang-Song
ZS
Li
Hong-Qiang
HQ
Liu
Qing-Guo
QG
Qi
Jun-Yuan
JY
Liu
Qiang
Q
Chu
Yu-Lin
YL
Zhang
Feng-Kui
FK
D000741:Anemia, Aplastic / Q000628:therapy; D001772:Blood Cell Count; D001854:Bone Marrow Cells; D006801:Humans; D007165:Immunosuppression Therapy; D010198:Pancytopenia