Antibiotic-loaded chitosan film for infection prevention: A preliminary in vitro characterization.
The growing infection rate by methicillin-resistant Staphylococcus aureus, especially with bone fracture fixation implants, is a major concern in extremity musculoskeletal wound treatment. This preliminary investigation evaluates the ability of chitosan film to be loaded with daptomycin and vancomycin in the operating room, in situ loading, and applied to musculoskeletal fixation devices to lessen or prevent infection. Films with 61, 71, and 80% degrees of deacetylation (DDA) made using lactic or acetic acid solvents were analyzed for their antibiotic uptake, elution, and activity along with film swelling ratio, ultimate tensile strength, Young's modulus, adhesive strength, and degradation. Chitosan films after 1 min of rehydration were able in a simulated, clinical setting to maintain mechanical integrity and adhesive strength to be applied to bone fracture fixation devices or implant surfaces. The film percent degradation increased with DDA increasing from 61 to 80%, but film degradation rate decreased in the presence of antibiotics. Eighty percent DDA chitosan films were optimal for absorbing and eluting antibiotics. Antibiotics eluted by the films were active against Staphylococcus aureus. These findings indicate that an 80% DDA chitosan film is potentially advantageous as a clinically adjunctive treatment in musculoskeletal injuries to lessen or prevent infections.
Department of Biomedical Engineering, University of Memphis, Memphis, TN, USA. jksmith@memphis.edu
J Keaton
JK
Smith
Joel D
JD
Bumgardner
Harry S
HS
Courtney
Mark S
MS
Smeltzer
Warren O
WO
Haggard
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D017576:Daptomycin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008422:Materials Testing; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009984:Orthopedic Fixation Devices; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013718:Tensile Strength; D014640:Vancomycin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D014640:Vancomycin; D048271:Chitosan; D017576:Daptomycin