Resveratrol treatment induces redox stress in red blood cells: a possible role of caspase 3 in metabolism and anion transport.
Resveratrol, an important phytoalexine found in many plants, has been shown to be significantly effective in the treatment of several pathological conditions such as cancer, coronary heart disease and osteoarthritis. This study focuses on the effects of this drug on human red blood cells. In particular, we have examined the influence of resveratrol on Band 3, the anion exchanger protein, and hemoglobin as a function of the oxygenation-deoxygenation cycle. Moreover, special attention has been given to the metabolic changes imposed by caspase 3 activation. Resveratrol has proved to lower superoxide production, thereby decreasing heme-iron oxidation and saving the reducing power required for met-hemoglobin reduction. Oxygen binding experiments showed that resveratrol interacts with hemoglobin, shifting the T→R conformational transition towards the higher-affinity R state. This might contribute to altering the metabolic balance of the cell through an intensification of the pentose phosphate pathway. Moreover, at high oxygenation levels of the erythrocytic hemoglobin, resveratrol induces a significant activation of caspase 3, the action of which on Band 3 has a strong impact on cellular metabolism and anion transport.
Galtieri
Antonio
A
Department of Organic and Biological Chemistry, University of Messina, Salita Sperone 31, I-98166 Messina, Italy. agaltieri@unime.it
Tellone
Ester
E
Ficarra
Silvana
S
Russo
Annamaria
A
Bellocco
Ersilia
E
Barreca
Davide
D
Scatena
Roberto
R
Laganà
Giuseppina
G
Leuzzi
Ugo
U
Giardina
Bruno
B
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D001457:Anion Exchange Protein 1, Erythrocyte; D013267:Stilbenes; D053148:Caspase 3; D000077185:Resveratrol