Disruption of two defensive signaling pathways by a viral RNA silencing suppressor.
The Cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). We used microarrays to investigate defensive gene expression in 2b-transgenic Arabidopsis thaliana plants. Surprisingly, 2b inhibited expression of few SA-regulated genes and, in some instances, enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (JA). Consistent with this, infection of plants with CMV, but not the 2b gene-deletion mutant CMVDelta2b, strongly inhibited JA-inducible gene expression. JA levels were unaffected by infection with either CMV or CMVDelta2b. Although the CMV-Arabidopsis interaction is a compatible one, SA accumulation, usually considered to be an indicator of plant resistance, was increased in CMV-infected plants but not in CMVDelta2b-infected plants. Thus, the 2b protein inhibits JA signaling at a step downstream of JA biosynthesis but it primes induction of SA biosynthesis by another CMV gene product or by the process of infection itself. Like many plant viruses, CMV is aphid transmitted. JA is important in plant defense against insects. This raises the possibility that disruption of JA-mediated gene expression by the 2b protein may influence CMV transmission by aphids.
Lewsey
Mathew G
MG
Department of Plant Sciences, University of Cambridge, UK.
Murphy
Alex M
AM
Maclean
Daniel
D
Dalchau
Neil
N
Westwood
Jack H
JH
Macaulay
Keith
K
Bennett
Mark H
MH
Moulin
Michael
M
Hanke
David E
DE
Powell
Glen
G
Smith
Alison G
AG
Carr
John P
JP
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D017799:Cucumovirus / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
C404227:2b protein, cucumber mosaic virus; D003517:Cyclopentanes; D054883:Oxylipins; D012367:RNA, Viral; D014764:Viral Proteins; C011006:jasmonic acid; D020156:Salicylic Acid