Estimating prion concentration in fluids and tissues by quantitative PMCA.
Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.
Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School, Houston, Texas, USA.
Baian
B
Chen
Rodrigo
R
Morales
Marcelo A
MA
Barria
Claudio
C
Soto
D000818:Animals; D006224:Cricetinae; D008647:Mesocricetus; D018620:PrPSc Proteins / Q000097:blood* / Q000652:urine*; D017510:Protein Folding; D012608:Scrapie / Q000097:blood* / Q000652:urine*
D018620:PrPSc Proteins