Genistein inhibits Vibrio vulnificus adhesion and cytotoxicity to HeLa cells.
Vibrio vulnificus (V. vulnificus) is a useful model for bacterial septicemia as the bacterial infection generates a wide pathogenic spectrum in addition to a high mortality rate. This study was aimed at investigating the effects of genistein on the growth, cytotoxicity, adhesion, and the mouse mortality caused by V. vulnificus. The results of our study indicated that genistein (50 or 100 mg/L) effectively minimized the morphologic damages and inhibited acute death of HeLa cells by V. vulnificus. Strikingly, genistein significantly inhibited the adhesion of V. vulnificus to HeLa cells. This report confirmed that genistein showed bacteriostatic activity against V. vulnificus, but it did not exhibit any bactericidal activity. Nevertheless, genistein (20 mg/kg) effectively decreased CD-1 mice mortality caused by V. vulnificus infection.
Department of Oriental Medicine Materials, Dongshin University, Naju, Korea.
Dool-Ri
DR
Oh
Jong Ro
JR
Kim
Young Ran
YR
Kim
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001422:Bacterial Adhesion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008826:Microbial Sensitivity Tests / Q000379:methods; D014735:Vibrio Infections / Q000188:drug therapy* / Q000401:mortality; D041261:Vibrio vulnificus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity
D000900:Anti-Bacterial Agents; D019833:Genistein