Modeling the time evolution of the nanoparticle-protein corona in a body fluid.
BACKGROUND
Nanoparticles in contact with biological fluids interact with proteins and other biomolecules, thus forming a dynamic corona whose composition varies over time due to continuous protein association and dissociation events. Eventually equilibrium is reached, at which point the continued exchange will not affect the composition of the corona.


RESULTS
We developed a simple and effective dynamic model of the nanoparticle protein corona in a body fluid, namely human plasma. The model predicts the time evolution and equilibrium composition of the corona based on affinities, stoichiometries and rate constants. An application to the interaction of human serum albumin, high density lipoprotein (HDL) and fibrinogen with 70 nm N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles is presented, including novel experimental data for HDL.


CONCLUSIONS
The simple model presented here can easily be modified to mimic the interaction of the nanoparticle protein corona with a novel biological fluid or compartment once new data will be available, thus opening novel applications in nanotoxicity and nanomedicine.
Institute of Biology and Environmental Sciences, Section of Biochemistry, University of Oldenburg, Oldenburg, Germany. daniele.dellorco@uni-oldenburg.de
Daniele
D
Dell'Orco
Martin
M
Lundqvist
Cecilia
C
Oslakovic
Tommy
T
Cedervall
Sara
S
Linse
D001826:Body Fluids; D006801:Humans; D008962:Models, Theoretical; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry*
D011506:Proteins