Muscle-derived Gr1(dim)CD11b(+) cells enhance neovascularization in an ischemic hind limb mouse model.
Gr1(+)CD11b(+) cells are characterized as myeloid-derived suppressor cells potentially involved in angiogenesis. We demonstrate that Gr1(+)CD11b(+) cells isolated from ischemic muscle in a hind-limb ischemic C57BL/6 mouse model play a role in vessel formation after ischemic injury. Gr1(dim)CD11b(+) cells, a subpopulation of Gr1(+)CD11b(+) cells, within skeletal muscle were increased in context of ischemia. Strikingly, astrocyte-plexus formed from muscle-derived Gr1(dim)CD11b(+) cells in Matrigel culture, followed by formation of isolectin and von Willebrand Factor-expressing cells, similar to that reported for angiogenesis in retina. When isolated muscle-derived Gr1(dim)CD11b(+) cells were injected into ischemic muscles, recovery of blood flow was significantly enhanced and these cells were incorporated into vessel walls. This suggests that Gr1(dim)CD11b(+) cells are recruited into ischemic regions after ischemia and may be involved in angiogenesis by their capacity to generate vascular cells.
Kim
Jeong A
JA
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, 46202, USA.
March
Keith
K
Chae
Hee-Don
HD
Johnstone
Brian
B
Park
Su-Jung
SJ
Cook
Todd
T
Merfeld-Clauss
Stephanie
S
Broxmeyer
Hal E
HE
D000818:Animals; D015153:Blotting, Western; D039481:CD11b Antigen / Q000378:metabolism*; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006614:Hindlimb / Q000098:blood supply* / Q000503:physiopathology; D007511:Ischemia / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D012333:RNA, Messenger / Q000235:genetics; D019707:Receptors, Chemokine / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D012333:RNA, Messenger; D019707:Receptors, Chemokine