Characterization of a 6K oligonucleotide turkey skeletal muscle microarray.
Consumer demand for lean, inexpensive meat products has driven the domestic turkey (Meleagris gallopavo) industry to unprecedented production; however, this has coincided with an increase in growth-induced myopathies and meat quality defects. With the aim of developing a new tool for the study of turkey growth and development at the muscle transcriptome level, a 6K oligonucleotide microarray was constructed, the Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray. Skeletal muscle samples were collected at three critical stages in muscle development: 18-day embryo (hyperplasia), 1-day post-hatch (hypertrophy), and 16-week (market age) from two genetic lines of turkeys: RBC2, a line maintained without selection pressure, and F, a line selected from the RBC2 line for increased 16-week body weight. Oligonucleotides were designed from sequences obtained from skeletal muscle cDNA libraries from the three developmental stages. Several unique controls, including mismatch and distance controls and scrambled sequences, were designed for 30 genes. Quality control hybridizations were completed, confirming the validity and repeatability of the array. Control features were evaluated across two larger experiments comparing developmental stage within genetic line or genetic line within each developmental stage, totaling 70 arrays. Mismatch and scrambled sequences appeared to be useful controls of specific hybridization for most genes. In addition, quantitative real-time RT-PCR confirmed microarray results. This creation and assessment of the TSKMLO array provides a valuable community resource for the study of gene expression changes related to turkey muscle growth and development.
Sporer
K R B
KR
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
Chiang
W
W
Tempelman
R J
RJ
Ernst
C W
CW
Reed
K M
KM
Velleman
S G
SG
Strasburg
G M
GM
D000818:Animals; D020869:Gene Expression Profiling / Q000662:veterinary*; D015723:Gene Library; D008460:Meat; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D009135:Muscular Diseases / Q000662:veterinary; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000662:veterinary*; D014422:Turkeys / Q000235:genetics* / Q000254:growth & development*