Potential of a metabolic gene (accA3) of M. leprae as a marker for leprosy reactions.
Understanding the mechanism(s) of reactions in leprosy remains a challenging task for both clinicians and basic scientists. While there is some understanding of host processes associated with different type of lepra reactions, there is very little information about bacterial factors triggering these inflammatory processes. This study is continuation of our earlier research programme on leprosy genomics in which significant transcription of 11 genes was observed during active disease and these included accA3 gene. In present study, we have investigated the potential of this gene or its gene product as molecular and or immunological marker for studying the reactions. Using quantitative Real-Time RT-PCR significant higher expression (mean log2 ratio=3.39) of accA3 was observed in specimens from leprosy reaction cases compared with cases without reactions. in silico homology model of this protein was analyzed for hydrophilic and B-cell epitope regions. Peptides with maximum antigenecity were selected, cloned, expressed and used to study sero-reactivity across the disease spectrum by indirect ELISA. While sero-reactivity was observed in leprosy cases the antibody levels did not vary significantly between the patient/s of same clinical type with and without reaction thereby indicating the limitation of this approach for this purpose. Measurement of transcription of this gene has, thus, potential as a molecular marker for monitoring the reactions.
Sharma
R
R
Department of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Dr M Miyazaki Marg, Tajganj, Agra-282 001, India.
Lavania
M
M
Chauhan
D S
DS
Katoch
K
K
Amresh
Pramod
Rakhi
Richa
Katoch
V M
VM
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers; D001706:Biopsy; D016022:Case-Control Studies; D019295:Computational Biology; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D006801:Humans; D007918:Leprosy / Q000276:immunology / Q000382:microbiology / Q000473:pathology*; D009166:Mycobacterium leprae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D001426:Bacterial Proteins; D015415:Biomarkers; D012329:RNA, Bacterial; C550276:accA3 protein, Mycobacterium leprae