Proteomic analysis of ACTN4-interacting proteins reveals it's a putative involvement in mRNA metabolism.
Alpha-actinin 4 (ACTN4) is an actin-binding protein. In the cytoplasm, ACTN4 participates in structural organisation of the cytoskeleton via cross-linking of actin filaments. Nuclear localisation of ACTN4 has also been reported, but no clear role in the nucleus has been established. In this report, we describe the identification of proteins associated with ACTN4 in the nucleus. A combination of two-dimensional gel electrophoresis (2D-GE) and MALDI-TOF mass-spectrometry revealed a large number of ACTN4-bound proteins that are involved in various aspects of mRNA processing and transport. The association of ACTN4 with different ribonucleoproteins suggests that a major function of nuclear ACTN4 may be regulation of mRNA metabolism and signaling.
Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av, 4, 194064 St Petersburg, Russia.
Mikhail
M
Khotin
Lidia
L
Turoverova
Vasilisa
V
Aksenova
Nikolai
N
Barlev
Veronika Viktorija
VV
Borutinskaite
Alexander
A
Vener
Olga
O
Bajenova
Karl-Eric
KE
Magnusson
George P
GP
Pinaev
Dmitri
D
Tentler
D000185:Actinin / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism; D006801:Humans; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C111453:ACTN4 protein, human; D016326:Extracellular Matrix Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D000185:Actinin