A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus.
Eight reference genes of Brassica napus were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) data, focusing on vegetative tissues and developing embryos. Analyses of expression stability indicated that UP1, UBC9, UBC21, and TIP41 were the top four choices as stably expressed reference genes for vegetative tissues, whereas ACT7, UBC21, TIP41, and PP2A were the top four choices for maturing embryos. In addition, radiolabeling of overall messenger RNA (mRNA) of maturing embryos indicated that the expression patterns of the top four ranked reference genes reflected the overall mRNA content changes in maturing embryos.
Agricultural Lipid Biotechnology Program, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6H 2P5.
Xue
X
Chen
Martin
M
Truksa
Saleh
S
Shah
Randall J
RJ
Weselake
D029688:Brassica napus / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics; D020869:Gene Expression Profiling; D010940:Plant Proteins / Q000235:genetics* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012333:RNA, Messenger / Q000378:metabolism; D012015:Reference Standards
D010940:Plant Proteins; D012333:RNA, Messenger