Acinetobacter baylyi starvation-induced genes identified through incubation in long-term stationary phase.
Acinetobacter species encounter cycles of feast and famine in nature. We show that populations of Acinetobacter baylyi strain ADP1 remain dynamic for 6 weeks in batch culture. We created a library of lacZ reporters inserted into SalI sites in the genome and then isolated 30 genes with lacZ insertions whose expression was induced by starvation during long-term stationary phase compared with their expression during exponential growth. The genes encode metabolic, gene expression, DNA maintenance, envelope, and conserved hypothetical proteins.
Lostroh
C Phoebe
CP
Department of Biology, Colorado College, Colorado Springs, Colorado 80903, USA.
Voyles
Bruce A
BA
D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D019677:Artificial Gene Fusion; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D007763:Lac Operon; D013312:Stress, Physiological; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001616:beta-Galactosidase