Mutually opposite effects of dopamine.HCl and chlorpromazine. HCl on the thickness of liposomal lipid bilayers.
The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of dopamine.HCl (DA) and chlorpromazin.HCl (CPZ). Radiationless energy transfer from the surface fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid, to the hydrophobic fluorescent probe, bispyrenylpropane (Py-Py), was used to examine the effects of DA and CPZ on the thickness (D) of the liposomal lipid bilayers prepared with total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from neuronal membranes. The thickness (D) of intact SPMVTL and SPMVPL (37 degrees C, pH 7.4) were 0.914 +/- 0.010 and 0.886 +/- 0.009 (arbitrary units, n = 5), respectively. DA decreased the thickness of both SPMVTL and SPMVPL in a dose-dependent manner with a significant decrease in thickness observed even at 40 x 10(-9) M and 40 x 10(-9) M, respectively. On the other hand, CPZ increased the thickness of both SPMVTL and SPMVPL in a dose-dependent manner with a significant increase in thickness observed even at 35 x 10(-5) M and 35 x 10(-5) M, respectively. The sensitivities to the decreasing and increasing effect of the membrane lipid bilayers thickness by DA and CPZ, respectively, differed according to the liposomes in descending order of SPMVPL and SPMVTL. The decreasing and increasing action of DA and CPZ, respectively, on the membrane thickness had many effects that may be responsible for the dopaminergic receptor-DA and -CPZ interaction as well as the protein-lipid interaction.
Jung
Tae-Sang
TS
Department of Dental Pharmacology and Biophysics, Pusan National University, Yangsan, Korea.
Kim
Jeong-Kuk
JK
Nam
Ki Yong
KY
Lee
Kyung-Zoo
KZ
Lee
Sung-Key
SK
Park
Wang Bong
WB
Ko
Myung-Suk
MS
Kim
Won-Il
WI
Kim
Byung-Kug
BK
Kim
Byung Soo
BS
Jeon
Young Chan
YC
Chung
In Kyo
IK
Bae
Moon Kyoung
MK
Jang
Hye-Ock
HO
Yun
Il
I
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D002746:Chlorpromazine / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004735:Energy Transfer / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000138:chemical synthesis / Q000648:ultrastructure*; D010743:Phospholipids / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008055:Lipids; D008081:Liposomes; D010743:Phospholipids; D011721:Pyrenes; C027132:1-anilino-8-naphthalenesulfonate; D002746:Chlorpromazine; D004298:Dopamine