Astaxanthin restores the enzymatic antioxidant profile in salivary gland of alloxan-induced diabetic rats.
OBJECTIVE
To evaluate the effect of astaxanthin on antioxidant parameters of salivary gland from diabetic rats. The hypothesis of the study was whether the supplementation of diabetic rats with astaxanthin might antagonize, or at least prevent, the defect in their antioxidative status.


METHODS
Wistar rats (n=32) were divided in 4 groups: untreated control, treated control, untreated diabetic and treated diabetic rats. Astaxanthin (20mg/kg body weight) was administered daily by gavage for 30 days. On day 23, diabetes was induced by injection of alloxan (60 mg/kg body weight). After 7 days of diabetes induction, the rats were killed and submandibular and parotid removed. Superoxide dismutase (SOD), catalase, glutathione peroxidase and reductase activities and the content of thiol groups were determined. Data were compared by ANOVA and the Tukey test (p<0.05).


RESULTS
Diabetes caused a reduction of SOD, and thiol content and increase of catalase and glutathione peroxidase activities of submandibular gland whilst in the parotid gland diabetes caused an increase of thiol content and no effect in the antioxidant system. The astaxanthin restores the enzymatic activities in the salivary gland, however does not prevent its oxidative damage.


CONCLUSIONS
The submandibular gland presented more susceptibility to oxidative alterations induced by diabetes. Astaxanthin presented a positive effect on the oxidative protection of the salivary gland from diabetic rats.
Universidade Cruzeiro do Sul, CEP, São Paulo, Brazil. mariana.leite@cruzeirodosul.edu.br <mariana.leite@cruzeirodosul.edu.br>
Mariana Ferreira
MF
Leite
Amanda Martins de
AM
Lima
Monica Miyuki
MM
Massuyama
Rosemari
R
Otton
D000496:Alloxan; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000627:therapeutic use*; D002374:Catalase / Q000032:analysis / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000517:prevention & control*; D016166:Free Radical Scavengers / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis / Q000187:drug effects; D005980:Glutathione Reductase / Q000032:analysis / Q000187:drug effects; D008297:Male; D010306:Parotid Gland / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000187:drug effects; D013363:Submandibular Gland / Q000187:drug effects* / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis / Q000187:drug effects; D024341:Xanthophylls / Q000627:therapeutic use
D000975:Antioxidants; D016166:Free Radical Scavengers; D012471:Salivary Proteins and Peptides; D013438:Sulfhydryl Compounds; D024341:Xanthophylls; D000496:Alloxan; C005948:astaxanthine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase