Effects of oxygen concentration on the expression of an antioxidant gene in HepG2.
To clarify the effects of O(2) concentration (cO(2)) on antioxidant gene expression in human hepatocytes, mRNA expression of HepG2 cells cultured at 1, 3, and 5% cO(2) and atmospheric gas-phase, was measured. The expression of some genes fluctuated depending on the cO(2) in the incubator. This indicates that cO(2) is a critically important factor in the investigation of human biological mechanisms.
Sone
Yasuko
Y
Institute of Environmental Science for Human Life, Ochanomizu University, Tokyo, Japan.
Kudo
Yasuko
Y
Oba
Rieko
R
Otsuka
Yuzuru
Y
D000975:Antioxidants / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D010100:Oxygen / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; D012333:RNA, Messenger; D010100:Oxygen