A new pre-column derivatization method for determination of gabapentin in human serum by HPLC using UV detection.
A high-performance liquid chromatography method using UV detection for determination of gabapentin in human serum has been developed. Serum was deproteinized with acetonitrile, and supernatant was evaporated. Derivatization was performed using 4-nitrophenylisothiocyanate, and separation was achieved on a C(18) column with detection at 335 nm. The method was linear in the 0.12-6.0 microg/mL range. No interference was found from endogenous amino acids. The method is suitable for routine therapeutic drug monitoring and for pharmacokinetic studies.
Amini
Mohsen
M
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences.
Rouini
Mohammad-Reza
MR
Asad-Paskeh
Alireza
A
Shafiee
Abbas
A
D000588:Amines / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003509:Cyclohexanecarboxylic Acids / Q000097:blood* / Q000737:chemistry; D000077206:Gabapentin; D006801:Humans; D044967:Serum / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000097:blood* / Q000737:chemistry
D000588:Amines; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin