Measurements of movement and diffusion coefficients of single cells on polymeric surface from image analysis.
Time-lapse digital images were taken every 30 s of PC12 cells cultured on polystyrene dishes, collagen-coated dishes and poly(L-lysine)-coated dishes in high-serum medium, low-serum medium and neurite outgrowth factor (NGF)-containing medium to investigate their diffusion coefficients (i.e., self-diffusion coefficients), D and specific movement (i.e., specific frequent movement) using image analysis and Fast Fourier Transform (FFT) analysis. D for these cells was found to fluctuate as a function of time, D varying between 0 and 0.08 microm(2)/s. The trend observed upon examination of average D values was: D in high-serum medium > or = D in low-serum medium > or = D in NGF-containing medium. Image analysis and FFT analysis of single cells cultured on polymeric dishes in these three media did not have any specific frequency of cell movement between 0 and 0.0167 Hz. The high diffusion coefficient and high amplitude of power spectra of PC12 cells in high-serum medium might be attributed to the high energy necessary for their continual suppression of the mitogen-activated protein kinase (MAPK) cascade and for them to maintain their undifferentiated state.
Department of Chemical and Materials Engineering, National Central University, 300 Jhongda Road, Jhongli, Taoyuan 32001, Taiwan. higuchi@ncu.edu.tw
Akon
A
Higuchi
Ho
H
Ling-Yi
Li Ya
LY
Huang
Hui
H
Chen
Yi-Jen
YJ
Chen
Qing-Dong
QD
Ling
D000818:Animals; D002465:Cell Movement; D003094:Collagen; D004058:Diffusion; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008027:Light; D008853:Microscopy; D057054:Molecular Imaging; D016716:PC12 Cells; D011107:Polylysine; D011108:Polymers; D011137:Polystyrenes; D051381:Rats; D013057:Spectrum Analysis; D013499:Surface Properties; D013997:Time Factors
D011108:Polymers; D011137:Polystyrenes; D011107:Polylysine; D003094:Collagen