Inhibitory effect of glycoprotein isolated from Opuntia ficus-indica var. saboten MAKINO on activities of allergy-mediators in compound 48/80-stimulated mast cells.
The present study was performed to investigate the anti-allergy potentials of glycoprotein (90kDa) isolated from Opuntia ficus-indica var. saboten MAKINO (OFI glycoprotein) in vivo (ICR mice) and in vitro (RBL-2H3 cells). At first, to know whether the OFI glycoprotein has an inhibitory ability for allergy in vivo, we evaluated the activities of allergy-related factors such as histamine and beta-hexosaminidase release, lactate dehydrogenase (LDH), and interleukin 4 (IL-4) in compound 48/80 (8 ml/kg BW)-treated ICR mice. After that, we studied to found the effect for anti-allergy in vitro such as nuclear factor kappa B (NF-kappaB) and inducible nitric oxide synthase (iNOS), extracellular signal-regulated kinase (ERK) 1/2, arachidonic acid, and cyclooxygenase-2 (COX-2) in compound 48/80 (5 microg/ml)-treated RBL-2H3 cells. Our results showed that the OFI glycoprotein (5 mg/kg) inhibited histamine and beta-hexosaminidase release, lactate dehydrogenase (LDH), and interleukin 4 (IL-4) in mice serum. Also OFI glycoprotein (25 microg/ml) has suppressive effects on the expression of MAPK (ERK1/2), and on protein expression of anti-allergic proteins (iNOS and COX-2). Thus, we speculate that the OFI glycoprotein is an example of natural compound that blocks anti-allergic signal transduction pathways.
Lim
Kye-Taek
KT
Molecular Biochemistry Laboratory, Biotechnology Research Institute, Chonnam National University, Gwang-ju 300, Yongbong-Dong 500-757, Republic of Korea. ktlim@chonnam.ac.kr
D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006636:Histamine Release / Q000187:drug effects; D015847:Interleukin-4 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D029747:Opuntia / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000378:metabolism
D018926:Anti-Allergic Agents; D010940:Plant Proteins; D015847:Interleukin-4; D003189:p-Methoxy-N-methylphenethylamine; D007770:L-Lactate Dehydrogenase; D001619:beta-N-Acetylhexosaminidases