Lymphocyte Responses to Chymotrypsin- or Trypsin V-Digested beta-Lactoglobulin in Patients with Cow's Milk Allergy.
: Chymotrypsin- or trypsin V- (a mixture of trypsin and chymotrypsin) digested beta-lactoglobulin (BLG) peptides were prepared and were confirmed to have much less immunoglobulin (lg)G and lgE reactivity compared with intact BLG by IgG inhibition enzymelinked immunosorbent assay and IgE dot blotting. The lymphocyte responses to intact BLG and these peptides were examined using peripheral blood mononuclear cells (PBMCs) from 10 patients with cow's milk allergy. The PBMCs from most patients had lower lymphocyte responses to chymotrypsin- and trypsin V-digested BLG peptides than those to intact BLG. However, PBMCs from one and two patients retained significant proliferative responses to both peptides and to only the former peptide, respectively. Interferon-c production stimulated by chymotrypsin-digested peptides was still detectable in all five patients tested. Chymotrypsindigested BLG reduced lgE reactivity but still induced some lymphocyte responses.
Kondo
Masashi
M
Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan; T, Nakano--Research and Development Department, Bean Stalk Snow Co, Ltd, Kawagoe, Japan. g2104012@guedu.cc.gifu-u.ac.jp.
Fukao
Toshiyuki
T
Shinoda
Shinji
S
Kawamoto
Norio
N
Kaneko
Hideo
H
Kato
Zenichiro
Z
Matsui
Eiko
E
Teramoto
Takahide
T
Nakano
Taku
T
Kondo
Naomi
N