[Monitoring of endothelial activation markers during physiological pregnancy].
OBJECTIVE
To establish endothelial activation markers which could uncover endothelial damage during physiological pregnancy.


METHODS
Prospective study.


METHODS
We examined 403 pregnant women with a physiological pregnancy. Venous blood samples were collected from the women at the beginning of the pregnancy, a second sample was collected in the interval 24-28 weeks gestation. Parameters were examined using methods: t-PA--ELISA, PAI-1--ELISA, vWF Ag--EIA ePCR--ELISA, MMP-2,9--ELISA with fluorogenic detection, TIMP-2--ELISA, endothelial microparticles - flow cytometry.


RESULTS
The level of vWF antigen increased during the entire course of pregnancy (in the I. trimester the average level was 152.32%, in the II. and III. trimester 173.34% and 216.20% respectively). At the same time, vWf activity also increased (I. trimester average level 130.20%, II. and III. trimester 150.09% and 181.91% respectively). The level of thrombomodulin significantly increased during pregnancy (I. trimester average level 19.05 ng/ml, II. and III. trimester 28.47 ng/ml and 39.86 ng/ml respectively). The level of soluble form of EPCR increased during pregnancy (I. trimester average level 201.76 ng/ml, II. and III. trimester 274.68 ng/ml and 324.07 ng/ml respectively). The level of PAI-1 increased during the entire course of pregnancy (I. trimester average level 36.14 ng/ml, II. and III. trimester 50.07 ng/ml and 60.12 ng/ml respectively). The level of t- PA did not change significantly during the course of pregnancy (I. trimester average level 2.48 ng/ml, II. and III. trimester 2.97 and 3.34 ng/ml respectively). The levels of MMP-2 (I. trimester average level 9043.76 RFU, II. and III. trimester 9315.38 and 8800.27 RFU respectively), MMP-9 (I. trimester average level 8371.90, II. and III. trimester 8290.81 and 7470.50 respectively), TIMP-2 (I. trimester average level 92.5 ng/ml, II. and III. trimester 98.5 and 96.5 ng/ml respectively) or endothelial microparticles (I. trimester average level 3838.38 particles/microl, II. and III. trimester 3836.59 and 3650.59 particles/microl respectively) did not change significantly throughout the individual trimesters.


CONCLUSIONS
We confirmed the hypothesis regarding the significant influence pregnancy has on changes in levels of these markers.
Procházková
J
J
Hemato-onkologická klinika FN a LF UP, Olomouc. prochazkovajana@hotmail.com
Dhaifallah
I
I
Mechurová
A
A
Pilka
R
R
Simetka
O
O
Slavík
L
L
Ulehlová
J
J
Lubuský
M
M
Procházka
M
M
D015703:Antigens, CD / Q000097:blood; D015415:Biomarkers / Q000097:blood; D000074283:Endothelial Protein C Receptor; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006801:Humans; D020782:Matrix Metalloproteinases / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D011247:Pregnancy / Q000097:blood*; D011264:Pregnancy Trimesters; D011956:Receptors, Cell Surface / Q000097:blood; D018180:Thrombomodulin / Q000097:blood; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000097:blood; D014841:von Willebrand Factor / Q000032:analysis
D015703:Antigens, CD; D015415:Biomarkers; D000074283:Endothelial Protein C Receptor; C478370:PROCR protein, human; D017395:Plasminogen Activator Inhibitor 1; D011956:Receptors, Cell Surface; D018180:Thrombomodulin; D014841:von Willebrand Factor; D019716:Tissue Inhibitor of Metalloproteinase-2; D010959:Tissue Plasminogen Activator; D020782:Matrix Metalloproteinases