Pre-treatment with capsaicin in a rat osteoarthritis model reduces the symptoms of pain and bone damage induced by monosodium iodoacetate.
A rat model of osteoarthritis was used to investigate the effect of pre-treatment with capsaicin on the symptoms of osteoarthritis induced by the injection of monosodium iodoacetate. This model mimics both histopathology and symptoms associated of human osteoarthritis. Injection of monosodium iodoacetate, an inhibitor of glycolysis, into the femorotibial joints of rodents promotes loss of articular trabecular bone and invokes pain symptoms similar to those noted in human osteoarthritis. Twenty rats were divided in two groups either receiving placebo or monosodium iodoacetate. Each group was subdivided in two groups either receiving pre-treatment with capsaicin two weeks before monosodium iodoacetate injection or not, resulting in four groups of five rats each. The impact of a single intra-articular administration of capsaicin (0.5%) on the generation of evoked mechanical pain (hind limb weight bearing, automated von Frey monofilament and RotaRod tests) and bone lesions (micro-CT scan radiographic analyses of bone structure) following monosodium iodoacetate-induced osteoarthritis in rats was determined. Evoked mechanical pain as monitored over a period of 4 weeks after monosodium iodoacetate injection was abolished in capsaicin pre-treated animals and pain values are comparable to those of capsaicin controls. Chronic joint pathological changes such as bone erosion and trabecular damage were significantly reduced by pre-treatment with a single administration of capsaicin. Decrease of bone volume was considerably ameliorated and trabecular connectivity was substantially better in capsaicin pre-treated animals. Capsaicin, an agonist activator of the vanilloid nociceptors (TRPV1), appears to be effective in protecting bone from arthritic damage. The present results support the hypothesis that capsaicin-sensitive sensory neurons contribute to bone lesions in the monosodium iodoacetate-induced osteoarthritis rat model.
Department of Anaesthesiology, Pain and Palliative Medicine, Radboud University Nijmegen Medical Centre, Geert Grootplein zuid 10, Nijmegen, The Netherlands.
Karel-Martijn
KM
Kalff
Mohammed
M
El Mouedden
Jan
J
van Egmond
Jan
J
Veening
Leo
L
Joosten
Gert Jan
GJ
Scheffer
Theo
T
Meert
Kris
K
Vissers
D000818:Animals; D001842:Bone and Bones / Q000473:pathology*; D002211:Capsaicin / Q000009:adverse effects / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D006614:Hindlimb / Q000473:pathology; D006801:Humans; D007270:Injections, Intra-Articular; D007461:Iodoacetates; D009619:Nociceptors / Q000473:pathology; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D010146:Pain / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D010329:Patella / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000502:physiology; D016474:Weight-Bearing / Q000502:physiology
D007461:Iodoacetates; D002211:Capsaicin