Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution.
We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).
Tang
Weng Lin
WL
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Li
Zhi
Z
Zhao
Huimin
H
D001419:Bacteria / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D019020:Directed Molecular Evolution; D006900:Hydroxylation; D009154:Mutation; D020578:Sphingomonas / Q000201:enzymology; D013237:Stereoisomerism; D013379:Substrate Specificity
D003577:Cytochrome P-450 Enzyme System