Treadmill pre-training suppresses the release of glutamate resulting from cerebral ischemia in rats.
This study was designed to investigate the neuroprotective effect of treadmill pre-training against the over-release of glutamate resulting from cerebral ischemia. Sprague-Dawley rats underwent 2 weeks of treadmill run-training before cerebral ischemia was performed by middle cerebral artery occlusion. The level of glutamate in brain extracellular fluid was detected before, during and after ischemia/reperfusion. The expression of metabotropic glutamate receptor-1 (mGluR1) mRNA in striatum was examined after ischemia for 80 min and reperfusion for 240 min. Neurological defect score and brain infarction volumes were measured. The treadmill pre-training significantly suppressed the release of glutamate, and reduced the expression of mGluR1 mRNA at 59% (P < 0.01) and 62% (P < 0.05), respectively, as compared with the ischemia group. The neurological defect score and infarction volume were significantly improved by 75% (P < 0.01) and 74% (P < 0.01), respectively, in the pre-training group, as compared to the ischemia group. Treadmill pre-training has a significant neuroprotective function against ischemia/reperfusion injury, by suppressing glutamate release resulting from cerebral ischemia, and this effect may be mediated by downregulation of mGluR1.
Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai, China.
Jie
J
Jia
Yong-Shan
YS
Hu
Yi
Y
Wu
Hui-Xian
HX
Yu
Gang
G
Liu
Da-Nian
DN
Zhu
Chun-Mei
CM
Xia
Zhi-Juan
ZJ
Cao
Xi
X
Zhang
Qing-Chuan
QC
Guo
D000818:Animals; D003342:Corpus Striatum / Q000502:physiology / Q000503:physiopathology; D005080:Exercise Test / Q000379:methods; D005110:Extracellular Space / Q000737:chemistry; D018698:Glutamic Acid / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000503:physiopathology*; D008297:Male; D023421:Models, Animal; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics; D015427:Reperfusion Injury / Q000473:pathology
D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D018698:Glutamic Acid