[Serum markers of oxidative stress and assisted reproduction procedures results in infertile patients with polycystic ovary syndrome and controls].
OBJECTIVE
To compare the serum levels of five markers of oxidative stress and assisted reproduction (AR) outcomes among infertile patients, with tubal and/or male factor and with polycystic ovary syndrome (PCOS).


METHODS
70 patients were included, 58 with tubal and/or male factor infertility and 12 with PCOS, who underwent controlled ovarian stimulation to perform intracytoplasmic sperm injection (ICSI). A blood sample was collected between the third and fifth day of the menstrual cycle in the month prior to ovarian stimulation. We analyzed the levels of malondialdehyde, hydroperoxides, protein oxidation products, glutathione and vitamin E, by reading the absorbance with a spectrophotometer and by high performance liquid chromatography (HPLC). Data were analyzed statistically by the Student's t-test and Fisher's exact test.


RESULTS
Significant increases in the body mass index, ovarian volume and number of antral follicles were observed in PCOS patients, as well as the use of a lower total dose of follicle stimulating hormone for these patients. There were no differences in the response to ovarian stimulation, in the results of AR or serum levels of malondialdehyde, hydroperoxides, advanced oxidation protein products, glutathione and vitamin E between groups.


CONCLUSIONS
The present data did not demonstrate a difference in the levels of serum markers of oxidative stress or in AR results when comparing non-obese infertile patients with PCOS and controls. These data suggest that the results of AR may not be compromised in this specific subgroup of patients with PCOS. However, interpretations of the action of oxidative stress on the results of AR are still not clear and the reproductive implications of oxidative stress need to be better evaluated.
Area de Concentração Biologia da Reprodução pelo Departamento de Ginecologia e Obstetrícia da Faculdade de Medicina de Ribeirão Preto - USP - Ribeirão Preto, SP, Brasil.
Jhenifer Kliemchen
JK
Rodrigues
Luciana Azôr
LA
Dib
Rui Alberto
RA
Ferriani
Alceu Afonso
AA
Jordão Junior
Paula Andrea de Albuquerque Sales
PA
Navarro
D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood* / Q000378:metabolism; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000378:metabolism; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted
D015415:Biomarkers