Biochemical imaging below the diffraction limit--probing cellular membrane related structures by tip-enhanced Raman spectroscopy (TERS).
A first vibrational mapping on the nanometer scale was performed on a protein (streptavidin) labelled supported phospholipid film by means of tip-enhanced Raman spectroscopy (TERS). For this purpose a TERS spectral map was measured on the biomembrane model, using a step size far below the diffraction limit. Considering the model composition, spectra were classified as either typical for lipids, proteins or both simultaneously, in a qualitative manner. Subsequently, the spectroscopic information was assigned with respect to the topographic features. Since a spatial differentiation between different compositional domains is difficult to achieve by topographic features only, the combination of morphology and spectral data enables a much more detailed characterization of biomembranes.
Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Jena, Germany.
René
R
Böhme
Dana
D
Cialla
Marc
M
Richter
Petra
P
Rösch
Jürgen
J
Popp
Volker
V
Deckert
D040761:Biomimetic Materials / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D008567:Membranes, Artificial; D008954:Models, Biological; D013059:Spectrum Analysis, Raman / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry
D008563:Membrane Lipids; D008565:Membrane Proteins; D008567:Membranes, Artificial; D053835:Unilamellar Liposomes; D019809:Streptavidin