Multi-channel microfluidic devices combined with electrospray ionization quadrupole time-of-flight mass spectrometry applied to the monitoring of glutamate release from neuronal cells.
This paper describes an integrated system combining microfluidic devices with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) for monitoring cellular chemical release. To demonstrate the feasibility of this new system, the reported carnosine-protection process against Abeta42-induced glutamate released from PC12 cells, was monitored. Poly-L-lysine coated microchannels were used to culture cells. A multi-channel miniature extraction chip (MEC) was integrated into the design to remove salts and protein interference effects. ESI-Q-TOF-MS was employed to realize semi-quantitative and highly sensitive qualitative analysis. The protective effect of carnosine against Abeta42-induced neurotoxicity was evaluated under different conditions in microchannels in parallel. The secretion product analysis, carried out by ESI-Q-TOF-MS, was accomplished in 5 min using only 2.5 microL of solvent. Furthermore, we show that integrated microfluidic devices have significant potential for the analysis of cellular secretions, as well as for medical screening tests and for the diagnosis of specific diseases.
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Huibin
H
Wei
Haifang
H
Li
Dan
D
Gao
Jin-Ming
JM
Lin
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D002336:Carnosine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000032:analysis* / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D018698:Glutamic Acid; D002336:Carnosine