Expression of a disintegrin and metalloproteinase 33 protein in nasal polyposis: an immunohistochemical study.
BACKGROUND
A disintegrin and metalloproteinase (ADAM)-33 is a member of matrix metalloproteinases. This protein takes a role in angiogenesis and airway remodeling in asthma. Because histopathological findings of airway remodeling in asthma and nasal polyposis (NP) are similar, the aim of this study was to evaluate the ADAM-33 expression in NP.


METHODS
Immunohistochemical staining of specimens of 47 patients with NP and 8 patients with concha bullosa was performed to detect the expression of ADAM-33. Paraffin blocks were used to identify the expression of ADAM-33 polyclonal antibodies. Immunostaining of epithelial cells, stroma, mesenchymal cells of vessels, and inflammatory cells were analyzed by using light microscopy.


RESULTS
Immunopositivity scores in epithelial cells in NP (median, 2; range, 1-3) were significantly higher than those of controls (median, 1.5; range, 1-2; p < 0.001). ADAM-33 staining was increased in the mesenchymal cells of vessels of nasal polyps (median, 2; range, 1-3) compared with control tissues (median, 1.5; range, 1-2; p = 0.006). Although the staining scores of fibroblasts in nasal polyp specimens were also high (median, 3; range 1-3), there was no statistical significance when compared with controls (median 2; range, 1-3; p = 0.228). ADAM-33 immunostaining was not related with the presence of allergies, asthma, and aspirin intolerance (p > 0.05). Moreover, no relationship was found between increased expression of ADAM-33 and the stages of polyp or computerized tomography scores (p > 0.05).


CONCLUSIONS
This study suggests that the increased expression of ADAM-33 protein may have a role in the pathogenesis of NP.
Department of Otolaryngology, Baskent University Faculty of Medicine, Ankara, Turkey. selimerbek@gmail.com
Selim S
SS
Erbek
Hilal
H
Erinanc
Seyra
S
Erbek
Ozgul
O
Topal
Halil
H
Kiyici
D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000328:Adult; D000368:Aged; D001808:Blood Vessels / Q000473:pathology; D018450:Disease Progression; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D007249:Inflammation; D008297:Male; D008648:Mesoderm / Q000378:metabolism* / Q000473:pathology; D046228:Microarray Analysis; D008875:Middle Aged; D009298:Nasal Polyps; D009666:Nose / Q000098:blood supply
D051722:ADAM Proteins; C450441:ADAM33 protein, human