Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus.
We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12-48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.
Todoroki
Miwako
M
Department of Mental Health, Institute of Industrial Ecological Sciences, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.
Ueta
Yoichi
Y
Fujihara
Hiroaki
H
Otsubo
Hiroki
H
Shibata
Minori
M
Hashimoto
Hirofumi
H
Kobayashi
Mizuki
M
Sakamoto
Hirotaka
H
Kawata
Mitsuhiro
M
Dayanithi
Govindan
G
Murphy
David
D
Hiro
Hisanori
H
Takahashi
Ken
K
Nagata
Shoji
S
D000818:Animals; D030801:Animals, Genetically Modified; D001127:Arginine Vasopressin / Q000235:genetics*; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics*; D007276:Injections, Intraventricular; D008125:Locus Coeruleus / Q000378:metabolism*; D009994:Osmolar Concentration; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012149:Restraint, Physical; D012892:Sleep Deprivation; D013315:Stress, Psychological; D013495:Supraoptic Nucleus / Q000378:metabolism
D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D001127:Arginine Vasopressin; D049452:Green Fluorescent Proteins; D003078:Colchicine