Interaction of an endosomolytic polyamidoamine ISA23 with vesicles mimicking intracellular membranes: A SANS/EPR study.
The mechanism of ISA23 · HCl interaction with model membrane vesicles (80-100 nm in diameter) was investigated using EPR in conjunction with SANS. For EPR, 16-DSE was dissolved in the vesicle membrane to measure its dynamics and polarity, whereas a spin-labeled (Tempo)-ISA 23 analogue was used to give a measure of the polymer flexibility. When ISA23 was added to the external vesicle surface, no interaction was found. This observation conflicts with the reported ability to lyse RBC, but is in agreement with recent studies that showed no effect on membrane permeability when a PAA was added to an incubation medium containing isolated lysosomal vesicles. The vesicle-mimetic models used here provide a new and useful tool for studying endosomolytic polymer/membrane interactions.
School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff CF10 3AT, UK. griffithsPC@cardiff.ac.uk
Peter C
PC
Griffiths
Renuka
R
Nilmini
Emma
E
Carter
Patrick
P
Dodds
Damien M
DM
Murphy
Zeena
Z
Khayat
Ettore
E
Lattanzio
Paolo
P
Ferruti
Richard K
RK
Heenan
Stephen M
SM
King
Ruth
R
Duncan
D032701:Biomimetics; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D011992:Endosomes / Q000737:chemistry*; D007425:Intracellular Membranes / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
C496056:ISA23 polymer; D010879:Piperazines; D011073:Polyamines