Comparative study of the effects of low-intensity pulsed ultrasound and low-level laser therapy on bone defects in tibias of rats.
The aim of this study was to investigate and to compare the effects of low intensity ultra-sound (LIPUS) and low-level laser therapy (LLLT) during the process of bone healing by means of histopathological and morphometric analysis. The animals were randomly distributed into three groups of 30 animals each: the control group (bone defect without treatment); the laser-treated group: (bone defect treated with laser), and the LIPUS-treated (bone defect treated with ultrasound). Each group was further divided into three different subgroups (n = 10) and on days 7, 13, and 25 post-injury, rats were killed with an intra-peritoneal injection of general anesthetic. The rats were treated with a 30-mW/cm(2) low-intensity pulsed ultrasound and a 830-nm laser at 50 J/cm(2). The results showed intense new bone formation surrounded by highly vascularized connective tissue presenting a slight osteogenic activity, with primary bone deposition being observed in the group exposed to laser in the intermediary (13 days) and late stages of repair (25 days). This was confirmed by morphometric analysis in which significant statistical differences (p < 0.05) were noticed when compared to the control. No remarkable differences were noticed in the specimens treated with ultrasound with regard to the amount of newly formed bone in comparison to the control group. Taken together, our results indicate that laser therapy improves bone repair in rats as depicted by histopathological and morphometric analysis, mainly at the late stages of recovery. Moreover, it seems that this therapy was more effective than US to accelerate bone healing.
Department of Physiotherapy, Federal University of São Carlos, Rodovia Washington Luís (SP-310), Km 235, São Carlos, SP, Brazil.
Elaine
E
Fávaro-Pípi
Suellen Maurin
SM
Feitosa
Daniel Araki
DA
Ribeiro
Paulo
P
Bossini
Poliani
P
Oliveira
Nivaldo A
NA
Parizotto
Ana Claudia Muniz
AC
Renno
D000818:Animals; D001861:Bone Regeneration / Q000528:radiation effects; D017102:Fracture Healing / Q000528:radiation effects*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013978:Tibial Fractures / Q000473:pathology / Q000532:radiotherapy* / Q000628:therapy*; D013997:Time Factors; D014464:Ultrasonic Therapy