Identification of an L-arabinose reductase gene in Aspergillus niger and its role in L-arabinose catabolism.
The first enzyme in the pathway for l-arabinose catabolism in eukaryotic microorganisms is a reductase, reducing l-arabinose to l-arabitol. The enzymes catalyzing this reduction are in general nonspecific and would also reduce d-xylose to xylitol, the first step in eukaryotic d-xylose catabolism. It is not clear whether microorganisms use different enzymes depending on the carbon source. Here we show that Aspergillus niger makes use of two different enzymes. We identified, cloned, and characterized an l-arabinose reductase, larA, that is different from the d-xylose reductase, xyrA. The larA is up-regulated on l-arabinose, while the xyrA is up-regulated on d-xylose. There is however an initial up-regulation of larA also on d-xylose but that fades away after about 4 h. The deletion of the larA gene in A. niger results in a slow growth phenotype on l-arabinose, whereas the growth on d-xylose is unaffected. The l-arabinose reductase can convert l-arabinose and d-xylose to their corresponding sugar alcohols but has a higher affinity for l-arabinose. The K(m) for l-arabinose is 54 + or - 6 mm and for d-xylose 155 + or - 15 mm.
Mojzita
Dominik
D
VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland.
Penttilä
Merja
M
Richard
Peter
P
D001089:Arabinose / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D007700:Kinetics; D008957:Models, Genetic; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013997:Time Factors; D014158:Transcription, Genetic; D014994:Xylose / Q000737:chemistry
D014994:Xylose; D001089:Arabinose