Protective effect of mycophenolate mofetil on mercuric chloride-induced nephrotoxicity in rats.
In the present study the effect of mycophenolate mofetil (MMF), an immunosuppressant, on mercuric chloride (HgCl(2))-induced nephrotoxicity in male Wistar rats was investigated. Animals (200-250 g) were divided into five groups and were subjected to a 6-day treatment schedule. The first (control) group received only vehicle without any active drug. The second to fifth groups were administered HgCl(2) challenge (single dose of 5 mg/kg, s.c.) on the fourth day. Additionally, the second group received distilled water (DW) on all 6 days and the third group was administered DW the initial 3 days and MMF (10 mg/kg b.i.d. by oral gavage) on days 4-6. The fourth group was given DW the initial 2 days and MMF on days 3-6 and the fifth group received MMF all 6 days. All animals were euthanized on the sixth day. It was found that HgCl(2) administration caused significant nephrotoxicity, as indicated by a rise in serum creatinine, blood urea and soluble intercellular adhesion molecule 1 (sICAM-1) concentrations, histopathological injury and increased oxidative stress (altered malondialdehyde and glutathione levels) as compared to the control group. Administration of MMF significantly ameliorated HgCl(2)-induced nephrotoxicity. The results suggest the potential of MMF in preventing the acute nephrotoxicity of HgCl(2).
Sarangi
S C
SC
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India.
Reeta
K H
KH
Dinda
A K
AK
Gupta
Y K
YK
D000818:Animals; D003404:Creatinine / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000517:prevention & control; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008627:Mercuric Chloride / Q000506:poisoning*; D009173:Mycophenolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000097:blood
D007166:Immunosuppressive Agents; D018799:Intercellular Adhesion Molecule-1; D008315:Malondialdehyde; D008627:Mercuric Chloride; D014508:Urea; D003404:Creatinine; D005978:Glutathione; D009173:Mycophenolic Acid