Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells.
Human embryonic stem cells (hESCs) have two properties of interest for the development of cell therapies: self-renewal and the potential to differentiate into all major lineages of somatic cells in the human body. Widespread clinical application of hESC-derived cells will require culture methods that are low-cost, robust, scalable and use chemically defined raw materials. Here we describe synthetic peptide-acrylate surfaces (PAS) that support self-renewal of hESCs in chemically defined, xeno-free medium. H1 and H7 hESCs were successfully maintained on PAS for over ten passages. Cell morphology and phenotypic marker expression were similar for cells cultured on PAS or Matrigel. Cells on PAS retained normal karyotype and pluripotency and were able to differentiate to functional cardiomyocytes on PAS. Finally, PAS were scaled up to large culture-vessel formats. Synthetic, xeno-free, scalable surfaces that support the self-renewal and differentiation of hESCs will be useful for both research purposes and development of cell therapies.
Melkoumian
Zara
Z
Corning Life Sciences, Corning Inc., Corning, New York, USA.
Weber
Jennifer L
JL
Weber
David M
DM
Fadeev
Andrei G
AG
Zhou
Yue
Y
Dolley-Sonneville
Paula
P
Yang
Jiwei
J
Qiu
Liqun
L
Priest
Catherine A
CA
Shogbon
Christopher
C
Martin
Arthur W
AW
Nelson
Jodelle
J
West
Peter
P
Beltzer
James P
JP
Pal
Santona
S
Brandenberger
Ralph
R
D000179:Acrylates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
D000179:Acrylates; D010455:Peptides; C036658:acrylic acid