Tailoring nanocrystalline diamond coated on titanium for osteoblast adhesion.
Diamond coatings with superior chemical stability, antiwear, and cytocompatibility properties have been considered for lengthening the lifetime of metallic orthopedic implants for over a decade. In this study, an attempt to tailor the surface properties of diamond films on titanium to promote osteoblast (bone forming cell) adhesion was reported. The surface properties investigated here included the size of diamond surface features, topography, wettability, and surface chemistry, all of which were controlled during microwave plasma enhanced chemical-vapor-deposition (MPCVD) processes using CH4-Ar-H2 gas mixtures. The hardness and elastic modulus of the diamond films were also determined. H2 concentration in the plasma was altered to control the crystallinity, grain size, and topography of the diamond coatings, and specific plasma gases (O2 and NH3) were introduced to change the surface chemistry of the diamond coatings. To understand the impact of the altered surface properties on osteoblast responses, cell adhesion tests were performed on the various diamond-coated titanium. The results revealed that nanocrystalline diamond (grain sizes <100 nm) coated titanium dramatically increased surface hardness, and the introduction of O2 and NH3 during the MPCVD process promoted osteoblast adhesion on diamond and, thus, should be further studied for improving orthopedic applications.
Division of Engineering, Brown University, Providence, Rhode Island 02912, USA.
Rajesh
R
Pareta
Lei
L
Yang
Abhishek
A
Kothari
Sirivisoot
S
Sirinrath
Xingcheng
X
Xiao
Brian W
BW
Sheldon
Thomas J
TJ
Webster
D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D018130:Diamond / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D005453:Fluorescence; D006244:Hardness / Q000187:drug effects; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D014867:Water; D018130:Diamond; D014025:Titanium