Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties.
In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immunophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.
Yap
Fei-Ling
FL
Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia. feiling_yap@imu.edu.my
Cheong
Soon-Keng
SK
Ammu
Radhakrishnan
R
Leong
Chooi-Fun
CF
D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D000954:Antigens, Surface; D015415:Biomarkers; D018664:Interleukin-12