[PI3-kinase mediates thrombin-induced platelet aggregation through mDia1 pathway.].
OBJECTIVE
To investigate the expression of mDia1 (mammalian diaphanous 1)in platelet and the role of mDia1 or phosphatidylinositol 3-kinase (PI3K) in the process of thrombin-induced platelet aggregation.


METHODS
The extent of platelet aggregation was measured by a platelet aggregation system and the expression of mDia1 and its relation with F-actin in quiescent, spreading or aggregated platelets by Western blot.


RESULTS
There was no significant difference in mDia1 expression level between quiescent and activated platelets. mDia1 moved from a Triton-X100-soluble cytosolic fraction to insoluble cytoskeleton fraction after thrombin induced platelets aggregation. Anti-mDia1 antibody could inhibit this aggregation. PI3K inhibitor Wortmannin or Ly294002 inhibited the thrombin induced platelet aggregation and the above mentioned mDia1 translocation.


CONCLUSIONS
PI3-kinase mediates the thrombin-induced platelet aggregation through mDia1 pathway.
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Guang-Xun
GX
Gao
Hong-Juan
HJ
Dong
Hong-Tao
HT
Gu
Ying
Y
Gao
Yao-Zhu
YZ
Pan
Yi-Wei
YW
Wang
Yang
Y
Yang
Xie-Qun
XQ
Chen
D000199:Actins; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D000199:Actins; D010975:Platelet Aggregation Inhibitors; D019869:Phosphatidylinositol 3-Kinases; D013917:Thrombin