Inhibitory effects of glutathione on dengue virus production.
Reduced glutathione (GSH) is the most powerful intracellular antioxidant and also involved in viral infections. The pathogenesis of dengue virus (DV) infection has not been completely clarified. This study investigated the relationship between DV serotype 2 (DV2) infections and host intracellular GSH content. Results showed infection with DV2 resulted in a decrease in intracellular GSH, which caused NF-kappaB activation and increased DV2 production. Supplemental GSH significantly inhibited activation of NF-kappaB, resulting in a decreased production of DV2 in HepG2 cells. Furthermore, high activity of NF-kappaB and increased production of DV2 was observed in HepG2 cells treated with buthionine sulfoximine (BSO), an inhibitor of GSH synthesis. In conclusion, DV2 infection could reduce host intracellular GSH concentration and benefited from this process. Supplemental GSH could inhibit viral production, indicating GSH might be valuable in the prevention and treatment of DV2 infection.
Department of Histology and Embryology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, PR China.
Yanping
Y
Tian
Wen
W
Jiang
Na
N
Gao
Junlei
J
Zhang
Wei
W
Chen
Dongying
D
Fan
Deshan
D
Zhou
Jing
J
An
D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002460:Cell Line; D003715:Dengue / Q000378:metabolism / Q000517:prevention & control / Q000821:virology*; D003716:Dengue Virus / Q000187:drug effects* / Q000502:physiology; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology*; D054884:Host-Pathogen Interactions; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D016328:NF-kappa B; D019328:Buthionine Sulfoximine; D005978:Glutathione