Porous poly(vinyl alcohol)-alginate gel hybrid construct for neocartilage formation using human nasoseptal cells.
BACKGROUND
Limited options exist for the restoration of craniofacial cartilage. Autologous tissue or porous polyethylene is currently used for nasal and auricular reconstruction. Both options are associated with drawbacks, including donor site morbidity and implant extrusion. Poly(vinyl alcohol) (PVA) is a non-degradable flexible biocompatible polymer than can be engineered to mimic the properties of cartilage. The goal of this study was to engineer a biosynthetic hybrid construct using a combination of PVA-alginate hydrogels and human nasal septum chondrocytes.


METHODS
Chondrocytes isolated from human nasal septum cartilage were expanded and mixed with 2% sodium alginate hydrogel. The chondrocyte-alginate mix was injected into a non-degradable porous PVA hydrogel, creating biosynthetic constructs. A group of these constructs were implanted into the subcutaneous environment of nude mice, while the other group was cultured in a spinner flask bioreactor system for 10 d and then implanted. After 6 wk in vivo, the histologic, biochemical, and biomechanical properties were examined.


RESULTS
Histological analysis demonstrated sulfated glycosaminoglycans and deposition of collagen type II in constructs from both groups. Constructs cultured in the bioreactor system prior in vivo implantation demonstrated higher levels of DNA, glycosaminoglycans, and hydroxyproline. An increase of 22% in the compressive strength of the engineered constructs exposed to the bioreactor was also observed.


CONCLUSIONS
A novel porous PVA-alginate gel hybrid was used to successfully engineer human cartilage in vivo. A 10-d period of bioreactor culturing increased levels of DNA, glycosaminoglycans, hydroxyproline, and the compressive modulus of the constructs.
Plastic Surgery Research Laboratory, Division of Plastic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
David A
DA
Bichara
Xing
X
Zhao
Nathaniel S
NS
Hwang
Hatice
H
Bodugoz-Senturk
Michael J
MJ
Yaremchuk
Mark A
MA
Randolph
Orhun K
OK
Muratoglu
D000328:Adult; D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D019149:Bioreactors; D002356:Cartilage / Q000166:cytology*; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006801:Humans; D051379:Mice; D009300:Nasal Septum / Q000166:cytology*; D011142:Polyvinyl Alcohol / Q000008:administration & dosage*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; C062364:polyvinyl alcohol hydrogel; D011142:Polyvinyl Alcohol