Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy.
For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microfluidics to observe optically trapped living cells in a minimally invasive fashion during laser micromanipulation. The obtained results demonstrate that DHM multi-focus phase contrast provides label-free quantitative monitoring of optical manipulation with a temporal resolution of a few milliseconds.
Center for Biomedical Optics and Photonics, University of Münster, Münster, Germany. bkemper@uni-muenster.de
Björn
B
Kemper
Patrik
P
Langehanenberg
Alexander
A
Höink
Gert
G
von Bally
Falk
F
Wottowah
Stefan
S
Schinkinger
Jochen
J
Guck
Josef
J
Käs
Ilona
I
Bredebusch
Jürgen
J
Schnekenburger
Karin
K
Schütze
D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D053685:Laser Therapy / Q000379:methods; D007834:Lasers; D044085:Microfluidics; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D010789:Phototherapy / Q000379:methods; D013997:Time Factors; D014466:Ultraviolet Rays