A novel material incorporation technique for medaka (Oryzias latipes) eggs using nanosecond pulsed electric fields.
The effects of nanosecond pulsed electric fields (nsPEFs) on medaka eggs were examined. Although embryogenesis was not affected by nsPEF treatment alone, significant harmful effects were observed when the eggs were treated with nsPEFs in the presence of cycloheximide in the outer solution. Nanosecond PEF treatment affected the permeability of both the egg envelope (chorion) and the cell membrane, which resulted in intracellular incorporation of cycloheximide.
Tominaga
Nobuaki
N
Department of Chemical and Biological Engineering, Ariake National College of Technology, Omuta, Japan. tominaga@ariake-nct.ac.jp
Kono
Susumu
S
Yamaguchi
Akemi
A
Akiyama
Hidenori
H
Arizono
Koji
K
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004560:Electricity; D047108:Embryonic Development / Q000187:drug effects; D005306:Fertilization / Q000187:drug effects; D009990:Oryzias / Q000196:embryology* / Q000502:physiology; D010063:Ovum / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D003513:Cycloheximide