Efficient cell-seeding into scaffolds improves bone formation.
Bone marrow stromal cells (BMSCs)/beta-tricalcium phosphate (beta-TCP) composites have attracted a great deal of attention in bone tissue engineering. If more effective bone regeneration is to be achieved, efficient cell-seeding systems need to be clarified. In this study, we investigated the number of cells contained in composites, and the in vitro/vivo osteogenic differentiation capacity of composites using 4 conventional systems of seeding rat BMSCs into beta-TCP: soak, low-pressure, pipette, and syringe systems. The highest number of cells was contained in the composites from the syringe group. Moreover, after two-week osteogenic induction in vitro, the composites in the syringe group exhibited the highest osteogenic potential, which continued at 8 weeks after subcutaneous implantation in vivo. Our results indicated that efficient and appropriate cell-seeding could improve in vitro/vivo bone formation in composites and thus make a potential clinical contribution to successful bone tissue engineering.
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
T
T
Hasegawa
M
M
Miwa
Y
Y
Sakai
T
T
Niikura
S Y
SY
Lee
K
K
Oe
T
T
Iwakura
M
M
Kurosaka
T
T
Komori
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002130:Calcium Phosphates; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D007101:Immersion; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D015675:Osteocalcin / Q000096:biosynthesis; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000637:transplantation; D040521:Subcutaneous Tissue / Q000601:surgery; D013594:Syringes; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds; D014618:Vacuum
D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D000469:Alkaline Phosphatase