Growth factors and gene expression of stem cells: bone marrow compared with peripheral blood.
OBJECTIVE
To evaluate and compare the presence of cytokines and growth factors in both bone marrow (BM) and peripheral blood.


METHODS
Samples of autogenous BM aspirate and peripheral blood (PB) from 7 patients ranging in age from 49 to 80 years were analyzed with real-time polymerase chain reaction technology to identify and compare selected gene expression for specific cytokines and growth factors. The genes selected for analysis included those involved in osteogenesis, hematogenesis, angiogenesis, extracellular matrix molecules, and cell-adhesion molecules. A maximum of 4 cc';s BM aspirate was taken from the anterior iliac crest and 0.5 mL of venous blood was drawn from each of 7 patients.


RESULTS
The results of the analysis indicate that both circulating blood and BM aspirate contain large quantities of a host of growth factors and cytokines. More platelet-derived growth factor is expressed in patient blood (PB) than in BM. Vascular endothelial growth factor alpha is expressed slightly greater in BM and vascular endothelial growth factor beta is slightly more prominent in PB. Transforming growth factors (TGFs) TGFA, TGFB1, and TGFB3 were equally expressed in BM and PB, and TGFB2 had a greater expression in PB. Bone morphogenetic proteins (BMPs) 1, 3, 7, 8B, R1A, and PR2 were almost equally expressed in BM and PB. BMPs 4 and 6 were expressed greater in PB. BMP2 was expressed more in BM. Extracellular matrix factors were equally expressed in PB and BM. Mesenchymal stem cell lineage markers varied in PB and BMA, and hematopoietic stem cell lineage markers were expressed more in PB than BM. Gene expression for angiogenic factors were equally expressed in PB and BM.


CONCLUSIONS
In this investigation, specific cytokines and growth factors in BM are compared with those in peripheral blood. Each has similar biologic effects and most expressed equally in BM and PB. However, BMP2 and vascular endothelial growth factor alpha had greater expression in BM.
Smiler
Dennis G
DG
Private practice, Encino, CA, USA. smiler@smiler.net
Soltan
Muna
M
Soltan
Christie
C
Matthews
Connie
C
D053687:Adult Stem Cells / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000235:genetics; D010012:Osteogenesis / Q000235:genetics
D016207:Cytokines; D006133:Growth Substances