Antibiotic resistance markers for genetic manipulations of Leptospira spp.
We measured the frequency of appearance of spontaneous mutants resistant to gentamicin, kanamycin, streptomycin, and spectinomycin in saprophytic and pathogenic Leptospira strains. The mutations responsible for the spontaneous resistance to streptomycin and spectinomycin were identified in the rpsL and rrs genes, respectively. We also generated a gentamicin resistance cassette that allows the use of a third selectable marker in leptospires. These results may facilitate further advances in gene transfer systems in Leptospira spp.
Poggi
Dante
D
Unité de Biologie des Spirochètes, Institut Pasteur, 75724 Paris Cedex 15, France.
Oliveira de Giuseppe
Priscila
P
Picardeau
Mathieu
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D007919:Leptospira / Q000187:drug effects* / Q000235:genetics*; D009154:Mutation; D012641:Selection, Genetic
D000900:Anti-Bacterial Agents