[Role of arginine in development of biopharmaceuticals].
Production, formulation and characterization of proteins play a major role in biotechnology industries. We have shown that aqueous arginine solution increases the solubility of proteins in formulation and purification, suppresses aggregation during refolding and storage and improves performance of column chromatography. Acidic arginine solution now finds application for virus inactivation. I will summarize these various effects of arginine on proteins and viruses and describe the current knowledge of the mechanism of the effects of arginine.
Arakawa
Tsutomu
T
Alliance Protein Laboratories, Thousand Oaks, CA 91360, USA. tarakawa2@aol.com
D000143:Acids; D000371:Agglutination; D001120:Arginine; D001702:Biopharmaceutics; D001709:Biotechnology; D002626:Chemistry, Pharmaceutical; D002845:Chromatography; D015195:Drug Design; D017510:Protein Folding; D011506:Proteins; D012996:Solutions; D038301:Virus Inactivation; D014867:Water
D000143:Acids; D011506:Proteins; D012996:Solutions; D014867:Water; D001120:Arginine