Matrix metalloproteinase-9 and -2 expression in the olfactory bulb following methyl bromide gas exposure.
Matrix metalloproteinase-9 (MMP-9) and MMP-2 are important for recovery following direct traumatic injury within the central nervous system (CNS). However, most CNS injury models include both direct trauma and neuronal deafferentation. This limits the ability to determine if these MMPs are important to one or both components of injury. To establish if MMPs play a role in the deafferentation processes, we investigated MMP-9 and MMP-2 in the olfactory bulb following methyl bromide gas exposure. This injury model lesions neurons within the olfactory epithelium and thereby leads to deafferentation of the bulb without damaging it directly. We measured the response of MMP-9 and MMP-2 in the olfactory bulb from 1 to 60 days during neuronal deafferentation and recovery. MMP-9 increased rapidly on day 5 and remained elevated for 10 days. MMP-2 expression levels were low compared with MMP-9. Immunohistological staining performed on days 1, 5, and 10 revealed MMP-9 was localized to inflammatory cells within the olfactory nerve and glomerular layers. Our results demonstrate MMP-9 is present in inflammatory cells during deafferentation processes in the olfactory bulb. Although MMP-9 is elevated in other CNS injury models, this is the first report to demonstrate an increase in MMP-9 associated with neuronal deafferentation in the absence of direct trauma.
Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street, Richmond, Virginia 23298, USA.
Stephen R
SR
Bakos
James E
JE
Schwob
Richard M
RM
Costanzo
D000818:Animals; D002490:Central Nervous System / Q000293:injuries; D005740:Gases / Q000737:chemistry / Q000633:toxicity*; D006842:Hydrocarbons, Brominated / Q000737:chemistry / Q000633:toxicity*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009830:Olfactory Bulb / Q000201:enzymology* / Q000473:pathology; D013997:Time Factors
D005740:Gases; D006842:Hydrocarbons, Brominated; C005218:methyl bromide; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9