Biodegradation of di-n-butyl phthalate in a soil microcosm.
This study investigated the effects of various culture treatments on di-n-butyl phthalate (DBP) degradation and the survival conditions of DBP-degrading bacterial strains in a soil microcosm. In the previous study, a DBP-degrading strain was isolated from activated sludge and identified by 16S rRNA as Deinococcus radiodurans. In this study, we added D. radiodurans into a soil microcosm and analyzed the structure of the whole bacterial community of the soil using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique. Meanwhile, the optimal conditions for DBP degradation were assessed by varying the temperature and initial pH of the culture, and by adding yeast extract and surfactants. The results show that the optimal conditions for DBP degradation in soil are a temperature of 35 degrees C, a pH of 7, and the addition of Triton X-100 and yeast extract. Furthermore, the addition of D. radiodurans can also enhance DBP degradation in soil. The PCR-DGGE analysis showed that D. radiodurans could survive in the soil microcosm through 24 days of incubation. We hope that these findings may provide some useful information about the remediation of DBP in our environment.
Liao
Chien-Sen
CS
Department of Civil and Ecological Engineering, I Shou University, Kaohsiung, Taiwan. csliao@isu.edu.tw
D001673:Biodegradation, Environmental; D034301:Deinococcus / Q000254:growth & development / Q000378:metabolism*; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004784:Environmental Monitoring; D006863:Hydrogen-Ion Concentration; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D015003:Yeasts / Q000502:physiology
D013501:Surface-Active Agents; D003993:Dibutyl Phthalate