Lifelong physical activity modulation of the skeletal muscle mitochondrial proteome in mice.
The aim of this study was to investigate the influence of lifestyle on the aging alterations in skeletal muscle mitochondrial proteins. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young, Y; old sedentary, S; and old active, A). The S and A mice were individually placed into standard cages and in cages with running wheels for 25 months. Upon killing, mitochondria from the hind limb skeletal muscles were isolated for the evaluation of general proteome alterations, carbonylation, and electron transport chain (ETC) activity. We identified 77 different proteins mostly from the oxidative phosphorylation and mitochondrial metabolism. Sedentary mice presented a significant loss of ETC functionality in opposition to active mice. Although some proteins were found damaged in both A and S mice, damage to ETC proteins was more evident in S. Moreover, it is also possible to conclude that lifestyle is a key modulator for preventing the aging-induced protein expression and functionality in mitochondria.
Departamento de Química, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Renato M P
RM
Alves
Rui
R
Vitorino
Pedro
P
Figueiredo
José Alberto
JA
Duarte
Rita
R
Ferreira
Francisco
F
Amado
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000375:Aging / Q000378:metabolism*; D000818:Animals; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008931:Mitochondria, Muscle / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D050050:Protein Carbonylation; D020543:Proteome
D024101:Mitochondrial Proteins; D020543:Proteome; D000255:Adenosine Triphosphate