Using the angle-dependent resonances of molded plasmonic crystals to improve the sensitivities of biosensors.
This paper describes how angle-dependent resonances from molded plasmonic crystals can be used to improve real-time biosensing. First, an inexpensive and massively parallel approach to create single-use, two-dimensional metal nanopyramidal gratings was developed. Second, although constant in bulk dielectric environments, the sensitivities (resonance wavelength shift and resonance width) of plasmonic crystals to adsorbed molecular layers of varying thickness were found to depend on incident excitation angle. Third, protein binding at dilute concentrations of protein was carried out at an angle that optimized the signal to noise of our plasmonic sensing platform. This angle-dependent sensitivity, which is intrinsic to grating-based sensors, is a critical parameter that can assist in maximizing signal to noise.
Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
Hanwei
H
Gao
Jiun-Chan
JC
Yang
Julia Y
JY
Lin
Andreea D
AD
Stuparu
Min Hyung
MH
Lee
Milan
M
Mrksich
Teri W
TW
Odom
D010367:Arachis / Q000737:chemistry*; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D055616:Microtechnology; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000191:economics / Q000295:instrumentation*
D004187:Disaccharides; D037102:Lectins; D010940:Plant Proteins