[Quantification of GRAF gene expression in patients with acute myeloid leukemia using EvaGreen real time quantitative PCR].
OBJECTIVE
To quantify the expression level of GRAF gene in acute myeloid leukemia (AML) and analyze its clinical significance.


METHODS
The EvaGreen real-time quantitative polymerase chain reaction (RQ-PCR) assay was established and performed to measure the GRAF gene transcripts in 71 cases with AML and 21 with nonmalignant hematological diseases. The clinical correlation of GRAF expression was analyzed.


RESULTS
The established EvaGreen RQ-PCR assay had good specificity, reproducibility and sensitivity. The GRAF expression level was significantly lower in AML (0.01%-169.75%, median 3.82%) than that in controls (14.49%-126.85%, median 56.04%) (P<0.05). There was no correlation between the level of GRAF transcript and the sex, age, hematologic parameters, FAB subtypes and karyotypic groups (P>0.05).


CONCLUSIONS
The GRAF gene was down-regulated in AML, which might play a role in the leukemogenesis.
Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002 PR China.
Zhen
Z
Qian
Jiang
J
Lin
Jun
J
Qian
Dong-ming
DM
Yao
Ya-li
YL
Wang
Lan-xiu
LX
Han
Zhao-hui
ZH
Zhu
Gao-fei
GF
Xiao
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D020690:GTPase-Activating Proteins / Q000235:genetics*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D055815:Young Adult
C115971:ARHGAP26 protein, human; D020690:GTPase-Activating Proteins