Identification of gibberellin acid-responsive proteins in rice leaf sheath using proteomics.
The phytohormone gibberellin acid (GA) controls many aspects of plant development. In this study, we identified proteins that are differentially expressed between the rice (Oryza sativa L.) GA-deficient cultivar, Aijiaonante, and its parental line, Nante. Proteins were extracted from rice leaf sheath and examined by 2DGE. Among more than 1200 protein spots reproducibly detected on each gel, 29 were found to be highly up-regulated by GAs in Nante, and 6 were down-regulated by GAs in Aijiaonante. These 35 proteins were identified by MALDI-TOF MS and were classified into three groups based on their putative function in metabolism, stress/defense processes and signal transduction. These data suggest that metabolic pathways are the main target of regulation by GAs during rice development. Our results provide new information about the involvement of GAs in rice development.
Gu
Jia-Yu
JY
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, PR China.
Wang
Ye
Y
Zhang
Xu
X
Zhang
Shi-Hua
SH
Gao
Yin
Y
An
Cheng-Cai
CC
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005875:Gibberellins / Q000378:metabolism / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D020543:Proteome / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018076:DNA, Complementary; D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing)