[Role of the interferon-stimulated response elements I/II in expression regulation of the retinoic acid induced gene G].
OBJECTIVE
To study the regulatory role of interferon-stimulated response elements (ISREs) located on the retinoic acid-induced gene G (RIG-G) promoter in RIG-G expression.


METHODS
By using point mutation technique, the authors constructed the wide type and site mutant reporter gene plasmids according to the ISRE sequence on RIG-G promoter, and detected the functional activities by luciferase reporter assay.


RESULTS
Mutation in ISRE II alone had no obvious effect on the expression of the reporter gene, whereas mutation in ISRE I dramatically inhibited the transactivity of RIG-G promoter. Mutation in both ISRE I and ISRE II resulted in complete loss of its response to the transcription factors for the reporter gene.


CONCLUSIONS
Both ISRE I and ISRE II on the RIG-G promoter are the binding sites for the complex of transcription factors. They are required for RIG-G expression, and ISRE I has a preferential role over ISRE II.
Lou
Ye-jiang
YJ
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025 PR China.
Pan
Xiao-rong
XR
Jia
Pei-min
PM
Zhang
Zhang-lin
ZL
Xu
Gui-ping
GP
Li
Dong
D
Tong
Jian-hua
JH
D045744:Cell Line, Tumor; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000235:genetics / Q000378:metabolism; D007372:Interferons / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D050795:STAT2 Transcription Factor / Q000235:genetics / Q000378:metabolism
C116468:IFIT3 protein, human; D050856:Interferon Regulatory Factor-1; D047908:Intracellular Signaling Peptides and Proteins; D050795:STAT2 Transcription Factor; C494084:STAT2 protein, human; D007372:Interferons