Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells.
The green alga Chlamydomonas reinhardtii is a model organism to study H(2) metabolism in photosynthetic eukaryotes. To understand the molecular mechanism of H(2) metabolism, we used 2-DE coupled with MALDI-TOF and MALDI-TOF/TOF-MS to investigate proteomic changes of Chlamydomonas cells that undergo sulfur-depleted H(2) photoproduction process. In this report, we obtained 2-D PAGE soluble protein profiles of Chlamydomonas at three time points representing different phases leading to H(2) production. We found over 105 Coomassie-stained protein spots, corresponding to 82 unique gene products, changed in abundance throughout the process. Major changes included photosynthetic machinery, protein biosynthetic apparatus, molecular chaperones, and 20S proteasomal components. A number of proteins related to sulfate, nitrogen and acetate assimilation, and antioxidative reactions were also changed significantly. Other proteins showing alteration during the sulfur-depleted H(2) photoproduction process were proteins involved in cell wall and flagella metabolisms. In addition, among these differentially expressed proteins, 11 were found to be predicted proteins without functional annotation in the Chlamydomonas genome database. The results of this proteomic analysis provide new insight into molecular basis of H(2) photoproduction in Chlamydomonas under sulfur depletion.
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Mei
M
Chen
Le
L
Zhao
Yong-Le
YL
Sun
Su-Xia
SX
Cui
Li-Fang
LF
Zhang
Bin
B
Yang
Jie
J
Wang
Ting-Yun
TY
Kuang
Fang
F
Huang
D016825:Chlamydomonas reinhardtii / Q000378:metabolism / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006859:Hydrogen / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology*; D010940:Plant Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012394:Rosaniline Dyes; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013455:Sulfur / Q000172:deficiency*
D010940:Plant Proteins; D012394:Rosaniline Dyes; D013455:Sulfur; C048139:Coomassie blue; D006859:Hydrogen