Molecular detection of poisonous mushrooms in different matrices.
Amanita phalloides, Lepiota cristata, Lepiota brunneoincarnata and Inocybe asterospora are among the most important species responsible of mushroom poisoning in northern Italy. A real time PCR method for the identification of samples containing DNA from each of these species was developed. To test specificity all protocols were applied on DNA extracted from various mushroom species; sensitivity was assessed performing serial dilutions on all samples; versatility of the protocols was evaluated performing tests on DNA extracted from different matrices. The protocols showed high sensitivity (32 ng dried mushroom), high specificity and sensitive detection of DNA extracted from difficult samples, including pasta with mushroom, cooked mushrooms and gastric aspirates.
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli studi di Milano, Milano, Italy. davide.sassera@unimi.it
Sara
S
Epis
Caterina
C
Matinato
Gabriella
G
Gentili
Fabio
F
Varotto
Claudio
C
Bandi
Davide
D
Sassera
D000363:Agaricales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000545:Amanita / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D007558:Italy; D009145:Mushroom Poisoning / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D017931:DNA Primers; D004271:DNA, Fungal