Electrospun alginate nanofibers with controlled cell adhesion for tissue engineering.
Alginate, a natural polysaccharide that has shown great potential as a cell scaffold for the regeneration of many tissues, has only been nominally explored as an electrospun biomaterial due to cytotoxic chemicals that have typically been used during nanofiber formation and crosslinking. Alginate cannot be electrospun by itself and is often co-spun with poly(ethylene oxide) (PEO). In this work, a cell adhesive peptide (GRGDSP) modified alginate (RA) and unmodified alginate (UA) were blended with PEO at different concentrations and blending ratios, and then electrospun to prepare uniform nanofibers. The ability of electrospun RA scaffolds to support human dermal fibroblast cell attachment, spreading, and subsequent proliferation was greatly enhanced on the adhesion ligand-modified nanofibers, demonstrating the promise of this electrospun polysaccharide material with defined nanoscale architecture and cell adhesive properties for tissue regeneration applications.
Department of Biomedical Engineering, Case Western Reserve University, Wickenden Building, Room 204, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
Sung In
SI
Jeong
Melissa D
MD
Krebs
Christopher A
CA
Bonino
Saad A
SA
Khan
Eben
E
Alsberg
D000464:Alginates; D002448:Cell Adhesion; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering
D000464:Alginates