Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Thymic stromal lymphopoietins (TSLPs) play critical roles in dendritic cell-mediated immune responses. In this study, we found that human trophoblasts and decidual epithelial cells in maternal-fetal interface of early placentas express TSLP mRNA and protein, but only trophoblast cells secret soluble TSLP. Human decidual CD1c(+) DCs (dDCs) highly express the functional TSLP receptor complex TSLP receptor and interleukin-7 receptor-α. Recombinant human TSLP activates CD1C(+) decidual DCs and peripheral monocyte-derived DCs with increased costimulatory molecules, major histocompatibility complex class II, and OX-40L. Human TSLP or supernatants from human trophoblasts specifically stimulate dDCs to highly produce interleukin-10 and T(H)2-attracting chemokine CCL-17. The TSLP-activated dDCs prime decidual CD4(+) T cells for T(H)2 cell differentiation, involved in maternal-fetal immunotolerance. Interestingly, the protein expression of TSLP in normal pregnancy with significant T(H)2 bias is much higher than that of miscarriage showing T(H)1 bias at the maternal-fetal interface. Therefore, human trophoblasts may contribute to maternal-fetal tolerance by instructing dDCs to induce regulatory T(H)2 bias in human early pregnancy via TSLP.
Guo
Pei-Fen
PF
Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics & Gynecology, Institute of Biomedical Sciences, Fudan University Shanghai Medical College, Shanghai, China.
Du
Mei-Rong
MR
Wu
Hai-Xia
HX
Lin
Yi
Y
Jin
Li-Ping
LP
Li
Da-Jin
DJ
D016207:Cytokines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003656:Decidua / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005333:Fetus / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D011247:Pregnancy / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D012995:Solubility; D018418:Th2 Cells / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology / Q000378:metabolism*; D000094632:Thymic Stromal Lymphopoietin
D016207:Cytokines; D012333:RNA, Messenger; D000094632:Thymic Stromal Lymphopoietin