Pomegranate (Punica granatum L.) juice supplementation attenuates isoproterenol-induced cardiac necrosis in rats.
The aim of the present study was to evaluate the efficacy of pre-supplementation with pomegranate (Punica granatum L.) juice (PJ) on heart weight, infarct size, plasma marker enzymes of cardiac damage, lipid peroxidation, endogenous enzymatic and non-enzymatic antioxidants, cardiac ATPases and histopathology of isoproterenol (IP)-induced cardiac necrosis (CN) in rats. Rats treated with IP (85 mg/kg, s.c.) for 2 days at an interval of 24 h caused significant (P < 0.05) infarct in myocardium and increase in heart weight, lipid peroxidation (LPO), activity levels of Ca(+2) ATPase and plasma marker enzymes, while there was significant (P < 0.05) decrease in endogenous enzymatic and non-enzymatic antioxidants and Na(+)-K(+) and Mg(+2)ATPases. Pre-supplementation with PJ for 30 consecutive days and treated with IP on days 29th and 30th showed significantly (P < 0.05) lesser increase in heart weight, infarct size, plasma marker enzymes, lipid peroxidation, Ca(+2) ATPase and a significant protective effect in endogenous enzymatic and non-enzymatic antioxidants, Na(+)-K(+) and Mg(+2) ATPases compared to IP alone treated group. Present study provides first scientific report on protective effect of supplementation of Pomegranate juice against IP-induced CN in rats.
Jadeja
Ravirajsinh N
RN
Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M S University of Baroda, Vadodara, 390002, GJ, India.
Thounaojam
Menaka C
MC
Patel
Dipak K
DK
Devkar
Ranjitsinh V
RV
Ramachandran
A V
AV
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001628:Beverages; D003402:Creatine Kinase / Q000378:metabolism; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D006331:Heart Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D007545:Isoproterenol / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D009336:Necrosis; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar
D000318:Adrenergic beta-Agonists; D000975:Antioxidants; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D000251:Adenosine Triphosphatases; D007545:Isoproterenol