[Effect of Liuwei Dihuang pill on expression of NF-kappaB in kidney lesion induced by gentamicin in rats].
OBJECTIVE
To study the effect of Liuwei Dihuang Pill on kidney lesion in rats induced by gentamicin and its mechanism.


METHODS
Rats were divided into three groups: control group, gentamicin group, Liuwei Dihuang Pill group randomly. Parameters of kidney function and oxidative production in three groups were tested by biochemical analysis. Nuclear Factor-kappaB (NF-kappaB) protein were determined by western blotting.


RESULTS
Parameters of weight, kidney indexes, oxidative production MDA and enzyme activity of NOS in kidney cortex of gentamicin group significantly increased (P < 0.05), and enzyme activity of SOD significantly decreased (P < 0. 05). The protein expression of NF-kappaB in gentamicin group was significantly up-regulated. After treatment with Liuwei Dihuang Pill, the above-mentioned up-regulated parameters were all significantly inhibited.


CONCLUSIONS
Liuwei Dihuang Pill can inhibit the expression of NF-kappaB protein, so as to alleviate the renal oxidative lesion, and it may be one of the important mechanisms for treating gentamicin induced kidney lesion.
Shanghai University of Traditional Chinese Medicine, College of nursing, Shanghai 201203, China.
Yan-Peng
YP
Hao
Yue
Y
Zhang
Yu-Min
YM
Liu
Min
M
Lu
Fei
F
Wang
Hai-Ying
HY
Lu
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005839:Gentamicins / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007677:Kidney Function Tests; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005839:Gentamicins; D016328:NF-kappa B; C476907:liu-wei-di-huang wan; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D048051:p38 Mitogen-Activated Protein Kinases