Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates.
Supported lipid bilayers (SLBs) mimic biological membranes and are a versatile platform for a wide range of biophysical research fields including lipid-protein interactions, protein-protein interactions and membrane-based biosensors. The quartz crystal microbalance with dissipation monitoring (QCM-D) has had a pivotal role in understanding SLB formation on various substrates. As shown by its real-time kinetic monitoring of SLB formation, QCM-D can probe the dynamics of biomacromolecular interactions. We present a protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and discuss technical issues that need to be considered when working with charged lipid compositions. Furthermore, we explain a recently developed strategy that uses an amphipathic, alpha-helical (AH) peptide to form SLBs on gold and titanium oxide substrates. The protocols can be completed in less than 3 h.
Cho
Nam-Joon
NJ
Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, California, USA. ncho@stanford.edu
Frank
Curtis W
CW
Kasemo
Bengt
B
Höök
Fredrik
F
D055592:Biophysical Phenomena; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold; D008051:Lipid Bilayers / Q000737:chemistry*; D008561:Membrane Fusion; D010087:Oxides; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D011791:Quartz; D017655:Silicon Compounds; D020349:Surface Plasmon Resonance / Q000379:methods; D014025:Titanium
D008051:Lipid Bilayers; D010087:Oxides; D010455:Peptides; D017655:Silicon Compounds; D011791:Quartz; C009495:titanium dioxide; C116473:silicon monoxide; D006046:Gold; D014025:Titanium