Rapid analysis of the main components of the total glycosides of Ranunculus japonicus by UPLC/Q-TOF-MS.
A rapid method for the analysis of the main components of the total glycosides of Ranunculus japonicus (TGOR) was developed using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS). The separation analysis was performed on a Waters Acquity UPLC system and the accurate mass of molecules and their fragment ions were determined by Q-TOF MS. Twenty compounds, including lactone glycosides, flavonoid glycosides and flavonoid aglycones, were identified and tentatively deduced on the basis of their elemental compositions, MS/MS data and relevant literature. The results demonstrated that lactone glycosides and flavonoids were the main constituents of TGOR. Furthermore, an effective and rapid pattern was established allowing for the comprehensive and systematic characterization of the complex samples.
Rui
Wen
W
Central laboratory, GuangDong Pharmaceutical University, Guangzhou, PR China.
Chen
Hongyuan
H
Tan
Yuzhi
Y
Zhong
Yanmei
Y
Feng
Yifan
Y
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D031947:Ranunculus / Q000737:chemistry*
D006027:Glycosides; D010936:Plant Extracts