β-ODAP accumulation could be related to low levels of superoxide anion and hydrogen peroxide in Lathyrus sativus L.
Level of the neuroexcitatory β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP) in grass pea (Lathyrus sativus L.) varies with development and environmental stress. Reactive oxygen species (ROS) (mainly O(2)ⁱ- and H(2)O(2)) are frequently reported to play important roles in plant development and in response to various stresses. To investigate the possible inter-relationship between contents of β-ODAP and ROS, grass pea leaves have been analyzed for contents of β-ODAP, O(2)ⁱ- and H(2)O(2). The results showed that leaves containing high levels of β-ODAP, exhibited low levels of O(2)ⁱ- and H(2)O(2), while leaves with high contents of O(2)ⁱ- and H(2)O(2) accumulated little β-ODAP. The application of pyridine or ABA which inhibit the production of O(2)ⁱ- or H(2)O(2) led to an increase in β-ODAP contents in intact or detached young leaves, whereas inhibition of catalase activity using AT (3-amino-1,2,4-triazole), leading to an increase in H(2)O(2) content, result in significant decrease in β-ODAP levels of detached young leaves. In addition, inoculation of Rhizobium to young seedlings enhanced O(2)ⁱ- and H(2)O(2) levels, but reduced β-ODAP contents in shoots. These results suggest that β-ODAP accumulation could be related to low levels of superoxide anion and hydrogen peroxide in grass pea tissues.
Jiao
C-J
CJ
Key Laboratory of Arid Agroecology under the Ministry of Education/Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China.
Jiang
J-L
JL
Li
C
C
Ke
L-M
LM
Cheng
W
W
Li
F-M
FM
Li
Z-X
ZX
Wang
C-Y
CY
D000599:Amino Acids, Diamino / Q000032:analysis* / Q000737:chemistry*; D000640:Amitrole / Q000378:metabolism; D002374:Catalase / Q000037:antagonists & inhibitors; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000032:analysis*; D027807:Lathyrus / Q000737:chemistry*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000737:chemistry; D012231:Rhizobium / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis*
D000599:Amino Acids, Diamino; D013481:Superoxides; C100126:oxalyldiaminopropionic acid; D006861:Hydrogen Peroxide; D002374:Catalase; D000640:Amitrole