The effects of 17beta-estradiol and vitamin E treatments on oxidative stress and antioxidant levels in brain cortex of diabetic ovariectomized rats.
We investigated the effects of 17beta-estradiol (E 2 ) and vitamin E on lipid peroxidation and antioxidant levels in brain cortex of diabetic ovariectomized (OVX) rats. Forty-two rats were equally divided into seven groups: Control; OVX; OVX+E 2 ; OVX+E 2 +vitamin E; OVX+diabetic; OVX+diabetic+E 2 and OVX+diabetic+E 2 +vitamin E. Then, E 2 (40 microg kg -1 /day) and vitamin E (100 mg kg -1 /day) were given. Glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), vitamin A and beta-carotene in brain were decreased (p<0.05) when compared with control in OVX but MDA in brain and glucose in plasma were elevated (p<0.05). The antioxidant levels in brain (p<0.05) increased although MDA (p<0.05) in brain and glucose (p<0.05, p<0.01) in plasma decreased in OVX after E 2 and E 2 +vitamin E supplementation. The activities of GSH-Px (p<0.001, p<0.01), CAT (p<005, p<0.001) and SOD (p<0.001) and the levels of GSH (p<0.001), vitamin A (p<0.05) and beta-carotene (p<0.001, p<0.05) were lower in the brain of OVX diabetic rats, while MDA in the brain and glucose in the plasma were higher (p<0.001). The antioxidant enzymes (p<0.05, p<0.01), GSH, vitamin A and beta-carotene (p<0.01, p<0.001) in brain increased while MDA in brain and glucose in plasma decreased (p<0.01, p<0.001) in diabetic OVX after treatments. Finally, the E 2 and E 2 +vitamin E supplementation to diabetic OVX and OVX rats may strengthen the antioxidant system by reducing lipid peroxidation.
Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey. ulas.mustafa@yahoo.com
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D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage*; D019207:beta Carotene / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D004958:Estradiol; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione