Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix.
This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.
Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
Yi
Y
Shao
Luowa
L
Quyang
Yueping
Y
Zhou
Jing
J
Tang
Yuehui
Y
Tan
Qiuping
Q
Liu
Zhirong
Z
Lin
Tingting
T
Yin
Fangfang
F
Qiu
Zuguo
Z
Liu
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology* / Q000378:metabolism*; D003319:Corneal Stroma / Q000166:cytology; D019573:Epithelium, Corneal / Q000166:cytology; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D012844:Sincalide / Q000378:metabolism; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D014867:Water / Q000378:metabolism
D001672:Biocompatible Materials; D014867:Water; D012844:Sincalide