[Higher percentages of T regulatory cells in children at risk for developing type 1 diabetes mellitus].
BACKGROUND
The natural history of type 1 diabetes is concerned with the appearance of autoantibodies against antigens of pancreatic beta cells. The last decade revealed some evidence of the participation of T regulatory lymphocytes - cells which suppress immune response - in the pathogenesis of type 1 diabetes and prediabetes.


OBJECTIVE
was the assessment of T regulatory cells in the blood of children at risk for developing type 1 the diabetes mellitus.


METHODS
85 subjects, siblings of children with type 1 diabetes, were enrolled into the study. The presence of anti-GAD65 antibodies was assessed. With the use of flow cytometry the following cell subpopulations were noted: CD4+, CD4+CD25high and CD4+CD25highCD127low with the coexpression of: CD28, CD45RO, CD54, CD62L and CD134 molecules.


RESULTS
We did not observe any differences in white blood cell count, lymphocyte (including CD4+) count and the percentage between the examined and control groups. We noted higher percentages of T regulatory cells: CD4+CD25high, CD4+CD127low and CD4+CD25highCD127low in children with the presence of anti-GAD65 antibodies as compared to the control children.


CONCLUSIONS
Higher percentages of T regulatory cells in the blood of children with the presence of anti-GAD65 antibodies may suggest an intensive regulatory response present in patients at risk for developing type 1 diabetes.
Luczyński
Włodzimierz
W
II Klinika Chorob Dzieci Uniwersytetu Medycznego w Bialymstoku. w.luczynski@wp.pl
Stasiak-Barmuta
Anna
A
Myśliwiec
Małgorzata
M
Nikołajuk
Agnieszka
A
Brandt
Agnieszka
A
Urban
Remigiusz
R
Kos
Justyna
J
Juchniewicz
Agnieszka
A
Jabłońska
Jolanta
J
Otocka
Agnieszka
A
Głowińska-Olszewska
Barbara
B
Florys
Bozena
B
Urban
Mirosława
M
Górska
Maria
M
Balcerska
Anna
A
D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D015703:Antigens, CD / Q000378:metabolism; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D015703:Antigens, CD; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2