Development of magnetic octadecylsilane particles as solid-phase extraction adsorbent for the determination of fat-soluble vitamins in fruit juice-milk beverage by capillary liquid chromatography.
A sensitive, fast and simple method based on magnetic octadecylsilane particles was developed for the extraction of three fat-soluble vitamins followed by capillary LC (CLC) analysis with UV detection. Magnetic octadecylsilane particles were prepared based on three-step reactions including co-precipitation, sol-gel polymerization and alkylation. The characterization of the prepared product was studied by scanning electron microscope and Fourier-transform infrared spectrometry. The particles were used as magnetic SPE adsorbent for the extraction of fat-soluble vitamins in fruit juice-milk beverage. The extraction condition and efficiency of the particles for fat-soluble vitamins were investigated. By coupling magnetic SPE with capillary LC with UV detection, low concentrations of fat-soluble vitamins in fruit juice-milk beverage can be detected without the interference from other substances in the sample matrix.
Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P. R. China.
Chaofan
C
Hu
Li
L
Jia
Qingqing
Q
Liu
Sheng
S
Zhang
D000327:Adsorption; D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D005223:Fats / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D008892:Milk / Q000737:chemistry*; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D014815:Vitamins / Q000032:analysis*
D005223:Fats; D005290:Ferric Compounds; D012821:Silanes; D014815:Vitamins; C024779:octadecylsilane; C000499:ferric oxide