Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus.
After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).
Zhang
Chun
C
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University, Chongqing, China.
Yang
Xiaolan
X
Feng
Juan
J
Yuan
Yonghua
Y
Li
Xiang
X
Bu
Youquan
Y
Xie
Yanling
Y
Yuan
Huidong
H
Liao
Fei
F
D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D004789:Enzyme Activation; D004795:Enzyme Stability; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D008297:Male; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D011994:Recombinant Proteins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013696:Temperature; D014503:Urate Oxidase / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*
D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D014503:Urate Oxidase