[Effect of Liuwei Dihuang pill on expression of NF-kappaB in kidney lesion induced by gentamicin in rats].
OBJECTIVE
To study the effect of Liuwei Dihuang Pill on kidney lesion in rats induced by gentamicin and its mechanism.


METHODS
Rats were divided into three groups: control group, gentamicin group, Liuwei Dihuang Pill group randomly. Parameters of kidney function and oxidative production in three groups were tested by biochemical analysis. Nuclear Factor-kappaB (NF-kappaB) protein were determined by western blotting.


RESULTS
Parameters of weight, kidney indexes, oxidative production MDA and enzyme activity of NOS in kidney cortex of gentamicin group significantly increased (P < 0.05), and enzyme activity of SOD significantly decreased (P < 0. 05). The protein expression of NF-kappaB in gentamicin group was significantly up-regulated. After treatment with Liuwei Dihuang Pill, the above-mentioned up-regulated parameters were all significantly inhibited.


CONCLUSIONS
Liuwei Dihuang Pill can inhibit the expression of NF-kappaB protein, so as to alleviate the renal oxidative lesion, and it may be one of the important mechanisms for treating gentamicin induced kidney lesion.
Hao
Yan-Peng
YP
Shanghai University of Traditional Chinese Medicine, College of nursing, Shanghai 201203, China.
Zhang
Yue
Y
Liu
Yu-Min
YM
Lu
Min
M
Wang
Fei
F
Lu
Hai-Ying
HY
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005839:Gentamicins / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007677:Kidney Function Tests; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005839:Gentamicins; D016328:NF-kappa B; C476907:liu-wei-di-huang wan; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D048051:p38 Mitogen-Activated Protein Kinases