An in vitro investigation of the mechanical-chemical and biological properties of calcium phosphate/calcium silicate/bismutite cement for dental pulp capping.
The properties of new calcium phosphate/calcium silicate/bismutite (CPCSBi) cement were compared with those of calcium hydroxide (CH) and Dycal cements in dental pulp-capping applications. CPCSBi is composed of hydroxyapatite, tetracalcium phosphate, bismutite, and calcium silicate, which was analyzed by SEM, FTIR, and XRD. The results of ion release from CPCSBi showed that the concentrations of Bi(3+), Ca(2+), PO4(2-), and Si(4+) increased with time in deionized water solutions. The setting time of CPCSBi and Dycal was 13 min 50 s and 2 min 25 s, respectively. There were no statistical differences in compressive strength and solubility between CPCSBi and Dycal (p > 0.05). The pH of CPCSBi (10.9) was lower than that of CH (11.6) and Dycal (12.5) after immersion for 24 h. Only slight cytotoxicity appeared for CPCSBi, whereas both CH and Dycal produced moderate discoloration and lysis. In antimicrobial tests against Sm, Av, La, and Sa, the antimicrobial potency of the CPCSBi was approximately 5-10 times greater than that of Dycal and CH groups. The dissoluble dentin matrix components (DDMCs) extracted from CPCSBi exposed to dentin powder demonstrated increased expression of dentin sialophosphoprotein (DSPP) and soteocalcin (OCN) dramatically in human pulp cells by RT-PCR. These results suggest that CPCSBi will be a good candidate for use as a dental pulp-capping agent in future.
Shanghai Biomaterials Research & Testing Center, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
Qingyi
Q
Shen
Jiao
J
Sun
Jie
J
Wu
Changsheng
C
Liu
Fangping
F
Chen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001729:Bismuth / Q000737:chemistry* / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology; D002478:Cells, Cultured; D019245:Compressive Strength; D003738:Dental Cements / Q000737:chemistry* / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000502:physiology; D003785:Dental Pulp Capping / Q000295:instrumentation / Q000379:methods; D003804:Dentin / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008903:Minerals / Q000737:chemistry / Q000494:pharmacology; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D003738:Dental Cements; D016326:Extracellular Matrix Proteins; D008903:Minerals; D017640:Silicates; C025702:Dycal; D002126:Calcium Hydroxide; C031293:calcium silicate; D001729:Bismuth