Gold-silver-alloy nanoprobes for one-pot multiplex DNA detection.
A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with Au-nanoprobes for a one-pot dual-colour detection strategy that allowed for the simultaneous differential detection of two distinct target sequences. This system poses an unprecedented opportunity to explore the combined use of metal nanoparticles with different composition towards the development of a multiplex one-pot colorimetric assay for DNA detection.
Centro de Investigação em Genética Molecular Humana (CIGMH), Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
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G
Doria
M
M
Larguinho
J T
JT
Dias
E
E
Pereira
R
R
Franco
P V
PV
Baptista
D003124:Colorimetry / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000032:analysis / Q000378:metabolism; D006047:Gold Alloys / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012834:Silver / Q000737:chemistry*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D015342:DNA Probes; D004277:DNA, Single-Stranded; D006047:Gold Alloys; D016159:Tumor Suppressor Protein p53; D012834:Silver