14-3-3 mediates histone cross-talk during transcription elongation in Drosophila.
Post-translational modifications of histone proteins modulate the binding of transcription regulators to chromatin. Studies in Drosophila have shown that the phosphorylation of histone H3 at Ser10 (H3S10ph) by JIL-1 is required specifically during early transcription elongation. 14-3-3 proteins bind H3 only when phosphorylated, providing mechanistic insights into the role of H3S10ph in transcription. Findings presented here show that 14-3-3 functions downstream of H3S10ph during transcription elongation. 14-3-3 proteins localize to active genes in a JIL-1-dependent manner. In the absence of 14-3-3, levels of actively elongating RNA polymerase II are severely diminished. 14-3-3 proteins interact with Elongator protein 3 (Elp3), an acetyltransferase that functions during transcription elongation. JIL-1 and 14-3-3 are required for Elp3 binding to chromatin, and in the absence of either protein, levels of H3K9 acetylation are significantly reduced. These results suggest that 14-3-3 proteins mediate cross-talk between histone phosphorylation and acetylation at a critical step in transcription elongation.
Department of Biology, Emory University, Atlanta, Georgia, United States of America.
Caline S
CS
Karam
Wendy A
WA
Kellner
Naomi
N
Takenaka
Alexa W
AW
Clemmons
Victor G
VG
Corces
D048948:14-3-3 Proteins / Q000378:metabolism*; D000107:Acetylation; D000818:Animals; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D051548:Histone Acetyltransferases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014158:Transcription, Genetic
D048948:14-3-3 Proteins; D029721:Drosophila Proteins; D006657:Histones; D009419:Nerve Tissue Proteins; C538730:ELP3 protein, Drosophila; D051548:Histone Acetyltransferases; C507306:JIL-1 protein, Drosophila; D017346:Protein Serine-Threonine Kinases