Chimeric antibodies against tick-borne encephalitis virus.
Two chimeric antibodies (ch) 13D6 and 10C2 against the glycoprotein E of tick-borne encephalitis virus (TBEV) were constructed by fusing variable regions of murine monoclonal antibodies (Mabs) 13D6 and 10C2 to human constant regions. Monovalent analogues of these antibodies in format of single-chain antibodies (scFv or sc) were developed, as well. The ch13D6, ch10C2, sc13D6 and sc10C2 exhibited binding characteristics similar to parental Mabs. Only the ch13D6 and sc13D6 were able to neutralize TBEV infectivity in vitro. The in vitro neutralization provided by ch13D6 suggests that this antibody can be further developed into a potent prophylaxis and therapy for tick-borne encephalitis (TBE) infection.
Levanov
Lev N
LN
Federal State Research Institution State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region 630559, Russia. levanov.lev@gmail.com
Matveev
Leonid E
LE
Goncharova
Elena P
EP
Lebedev
Leonid R
LR
Ryzhikov
Alexander B
AB
Yun
Tatyana E
TE
Batanova
Tatyana A
TA
Shvalov
Alexander N
AN
Baykov
Ivan K
IK
Shingarova
Lyudmila N
LN
Kirpichnikov
Michael P
MP
Tikunova
Nina V
NV
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus