Protease-activated receptor-2 (PAR(2)) in human periodontitis.
No evidence for the role of protease-activated receptor-2 (PAR(2)) in human periodontal disease has been demonstrated so far. Thus, we sought to investigate the expression of PAR(2) mRNA in chronic periodontitis, and to examine whether its expression is related to the presence of PAR(2) potential activators. Microbiological and gingival crevicular fluid samples were collected from individuals with chronic periodontitis and control individuals, and the presence of neutrophil serine proteinase 3 (P3) and Porphyromonas gingivalis was evaluated. PAR(2) mRNA expression was higher (p < 0.001) in those with chronic periodontitis compared with control individuals, and it was statistically decreased (p = 0.0006) after periodontal treatment. Furthermore, those with chronic periodontitis presented higher (p < 0.05) levels of IL-1alpha, IL-6, IL-8, and TNF-alpha, total proteolytic activity, P. gingivalis prevalence, and P3mRNA expression compared with control individuals. We conclude that PAR(2) mRNA expression and its potential activators are elevated in human chronic periodontitis, therefore suggesting that PAR(2) may play a role in periodontal inflammation.
Division of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, SP, Avenida Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo-SP, CEP: 05508-000, Brazil. marinella@usp.br
M
M
Holzhausen
J R
JR
Cortelli
V Araújo
VA
da Silva
G C Nobre
GC
Franco
S Cavalca
SC
Cortelli
N
N
Vergnolle
D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D055113:Chronic Periodontitis / Q000201:enzymology* / Q000382:microbiology* / Q000473:pathology / Q000628:therapy; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D053146:Myeloblastin / Q000032:analysis / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D044464:Receptor, PAR-2 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation; D055815:Young Adult
D007378:Interleukins; D012333:RNA, Messenger; D044464:Receptor, PAR-2; D014409:Tumor Necrosis Factor-alpha; D053146:Myeloblastin