Proteomics in detection and monitoring of asthma and smoking-related lung diseases.
Asthma, chronic obstructive pulmonary disease (COPD) and lung cancer cause extensive mortality and morbidity worldwide. However, the current state-of-the-art diagnosis and management schemes of these diseases are suboptimal as the incidence of asthma has risen by 250% over the last two decades and the 5-year mortality rate of lung cancer remains at 88%. Proteomic analysis is at the frontier of medical research and demonstrates tremendous potential in the early detection, diagnosis and staging, as well as providing novel therapeutic targets for improved management of smoking-related lung diseases. Advances in analytical tools, such as 2D gel electrophoresis, mass spectrometry, protein arrays and improved bioinformatics, allow sensitive and specific biomarker/protein profile discoveries and the infusion of new knowledge towards the molecular basis of lung diseases and their progression. Significant hurdles still stand between these laboratory findings and their applications in clinical practice. One of the challenges is the difficulty in the selection of samples that provide scope into the specific disease entity. Induced sputum, bronchoalveolar lavage, exhaled breath and exhaled breath condensate are methods of sampling airway and lung fluids that can serve as a window to assess the microenvironment of the lungs. With better study design standardization and the implementation of novel technologies to reach the optimal research standard, there is enough reason be optimistic about the future of proteomic research and its clinical implications.
Lin
Jiun-Lih
JL
Faculty of Medicine, University of New South Wales, Sydney, Australia.
Bonnichsen
Mark H
MH
Nogeh
Emily U
EU
Raftery
Mark J
MJ
Thomas
Paul S
PS
D001249:Asthma / Q000175:diagnosis* / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism; D006801:Humans; D010818:Practice Patterns, Physicians'; D040901:Proteomics / Q000379:methods*; D012907:Smoking / Q000009:adverse effects*; D013048:Specimen Handling