Phospholipase A(2) is required for PIN-FORMED protein trafficking to the plasma membrane in the Arabidopsis root.
Phospholipase A(2) (PLA(2)), which hydrolyzes a fatty acyl chain of membrane phospholipids, has been implicated in several biological processes in plants. However, its role in intracellular trafficking in plants has yet to be studied. Here, using pharmacological and genetic approaches, the root hair bioassay system, and PIN-FORMED (PIN) auxin efflux transporters as molecular markers, we demonstrate that plant PLA(2)s are required for PIN protein trafficking to the plasma membrane (PM) in the Arabidopsis thaliana root. PLA(2)alpha, a PLA(2) isoform, colocalized with the Golgi marker. Impairments of PLA(2) function by PLA(2)alpha mutation, PLA(2)-RNA interference (RNAi), or PLA(2) inhibitor treatments significantly disrupted the PM localization of PINs, causing internal PIN compartments to form. Conversely, supplementation with lysophosphatidylethanolamine (the PLA(2) hydrolytic product) restored the PM localization of PINs in the pla(2)alpha mutant and the ONO-RS-082-treated seedling. Suppression of PLA(2) activity by the inhibitor promoted accumulation of trans-Golgi network vesicles. Root hair-specific PIN overexpression (PINox) lines grew very short root hairs, most likely due to reduced auxin levels in root hair cells, but PLA(2) inhibitor treatments, PLA(2)alpha mutation, or PLA(2)-RNAi restored the root hair growth of PINox lines by disrupting the PM localization of PINs, thus reducing auxin efflux. These results suggest that PLA(2), likely acting in Golgi-related compartments, modulates the trafficking of PIN proteins.
School of Biological Sciences and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-742, Korea.
Ok Ran
OR
Lee
Soo Jin
SJ
Kim
Hae Jin
HJ
Kim
Jeum Kyu
JK
Hong
Stephen Beungtae
SB
Ryu
Sang Ho
SH
Lee
Anindya
A
Ganguly
Hyung-Taeg
HT
Cho
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000378:metabolism; D054467:Phospholipases A2 / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D021381:Protein Transport; D034622:RNA Interference; D021601:trans-Golgi Network / Q000378:metabolism
D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D026901:Membrane Transport Proteins; D054467:Phospholipases A2; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C449300:PIN3 protein, Arabidopsis