Electrospinning of matrigel to deposit a basal lamina-like nanofiber surface.
Schwann cell basal lamina is a nanometer-thin extracellular matrix layer that separates the axon-bound Schwann cells from the endoneurium of the peripheral nerve. It is implicated in the promotion of nerve regeneration after transection injury by allowing Schwann cell colonization and axonal guidance. Hence, it is desired to mimic the native basal lamina for neural tissue engineering applications. In this study, basal lamina proteins from BD Matrigel (growth factor-reduced) were extracted and electrospun to deposit nonwoven nanofiber mats. Adjustment of solute protein concentration, potential difference, air gap distance and flow rate produced a basal lamina-like construct with an average surface roughness of 23 nm and composed of 100-nm-thick irregular and relatively discontinuous fibers. Culture of embryonic chick dorsal root ganglion explants demonstrated that the fabricated nanofiber layer supported explant attachment, elongation of neurites, and migration of satellite Schwann cells in a similar fashion compared to electrospun collagen type-I fibers. Furthermore, the presence of nanorough surface features significantly increased the neurite spreading and Schwann cell growth. Sciatic nerve segment incubation also showed that the construct is promigratory to nerve Schwann cells. Results, therefore, suggest that the synthetic basal lamina fibers can be utilized as a biomaterial for induction of peripheral nerve repair.
Department of Biomedical Engineering, Wayne State University, Detroit, MI 48202, USA.
Roche C
RC
de Guzman
Jeffrey A
JA
Loeb
Pamela J
PJ
VandeVord
D000818:Animals; D001485:Basement Membrane / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D049108:Cell Enlargement / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D024042:Collagen Type I / Q000138:chemical synthesis / Q000737:chemistry; D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D004560:Electricity; D004625:Embryo, Nonmammalian; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007797:Laminin / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D011509:Proteoglycans / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012584:Sciatic Nerve / Q000166:cytology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014238:Trichloroacetic Acid / Q000138:chemical synthesis / Q000737:chemistry
D024042:Collagen Type I; D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D007797:Laminin; D011091:Polyesters; D011509:Proteoglycans; C059485:matrigel; C016240:polycaprolactone; D014238:Trichloroacetic Acid; D003094:Collagen