Single-molecule, real-time measurement of enzyme kinetics by alternating-laser excitation fluorescence resonance energy transfer.
Using a single-molecule fluorescence method uniquely suitable for binding assay, alternating-laser excitation fluorescence resonance energy transfer (ALEX-FRET), we accurately measured the cleavage rate of 8-17 deoxyribozyme, an RNA-cleaving enzyme, at the single-molecule level in real time with a minimum consumption of samples, i.e., at least three orders of magnitude smaller than used in the conventional ensemble FRET method.
Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Korea.
Nam Ki
NK
Lee
Hye Ran
HR
Koh
Kyu Young
KY
Han
Jihyun
J
Lee
Seong Keun
SK
Kim
D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D007834:Lasers; D012313:RNA / Q000737:chemistry / Q000378:metabolism
D021881:DNA, Catalytic; D012313:RNA