[Polar steroids from Solaster endeca starfish and the physiological activity of polar steroids from three starfish species].
Four polyhydroxylated steroids, new (20R)-5alpha-cholestan-3beta,6alpha,8,15alpha,24,26-hexaol (I) and known (20R,25S)-5alpha-cholestan-3beta6alpha,8,15beta,16beta,26-hexaol, (20R,25S)-5alpha-cholestan-3beta,6alpha,15beta,16beta,26-pentaol, and marthasterone sulfate were isolated from the Solaster endeca starfish inhabiting the Sea of Okhotsk and characterized. Steroid (I) contains a 24,26-dihydroxylated side chain, which is uncommon for starfish polyols. The isolated steroids and related metabolites from two starfish species of the Evasterias genus (in total, 15 compounds) were weakly cytotoxic in a human HeLa cell culture and some of them were inhibitors of nonspecific esterase from mouse Ehrlich carcinoma. The effects of these compounds on the p53 protein activity were studied in a yeast two-hybrid test system and both inhibitors and stimulators of this activity were found among them.
Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, pr. 100-Letiya Vladivostoka 159, Vladivostok, 690022 Russia. levina@piboc.dvo.ru
E V
EV
Levina
D L
DL
Aminin
S N
SN
Koval'chuk
V B
VB
Kozhemiako
S A
SA
Dyshlovoĭ
A I
AI
Kalinovskiĭ
P S
PS
Dmitrenok
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D004950:Esterases / Q000037:antagonists & inhibitors; D006801:Humans; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D016897:Respiratory Burst; D013215:Starfish / Q000737:chemistry*; D013237:Stereoisomerism; D013256:Steroids / Q000302:isolation & purification* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D000970:Antineoplastic Agents; D013256:Steroids; D016159:Tumor Suppressor Protein p53; D004950:Esterases