The establishment and characterization of adriamycin-resistant cell lines derived from Saos-2.
BACKGROUND
The aim was to establish and characterize adriamycin (ADM)-resistant cell lines.


METHODS
Two cell lines, named Saos-2/ADM1 and Saos-2/ADM4, resistant to ADM, were established after 167 days.


RESULTS
The resistance indices of methotrexate for the Saos-2/ADM1 and Saos-2/ADM4 cell lines to ADM were 49.8 and 74.6 times higher, respectively, than that of Saos-2. The two cells lines had resistance to MTX, IFO, EPI, THP, and PTX, while the cells were found to remain sensitive to cisplatin. Disordered and coenocytic structure were observed via microscopy.


CONCLUSIONS
This is the first report on ADM-resistant cells based on clinical peak doses in serum. MDR1 and MRP genes are involved in the resistance of cell lines to ADM, which are invaluable tools to study the resistance of anticancer drugs and to find the means to revert the resistance.
Niu
Bao-Hua
BH
Department of Pathology and Key Laboratory of Cellular and Molecular Immunology, School of Medicine, Henan University, Kaifeng, China.
Wang
Jian-Jun
JJ
Xi
Yan
Y
Ji
Xin-Ying
XY
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D020128:Inhibitory Concentration 50; D008727:Methotrexate / Q000494:pharmacology; D012516:Osteosarcoma / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D000970:Antineoplastic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D004317:Doxorubicin; C022616:thiazolyl blue; D002945:Cisplatin; D008727:Methotrexate