Time-lapse imaging of embryonic neural stem cell division in Drosophila by two-photon microscopy.
This unit describes a protocol for live imaging of Drosophila embryonic neural stem cells using two-photon microscopy. Compared to traditional one-photon confocal imaging, this technique renders higher-resolution optical sections from deeper within the embryo. It is ideally suited to following embryonic neuroblasts located underneath the neuroepithelial cell layer for several rounds of cell division.
Rebollo
Elena
E
Cell Division Group, IRB-Barcelona, Barcelona, Spain.
Gonzalez
Cayetano
C
D000818:Animals; D002455:Cell Division; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008297:Male; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000166:cytology*; D017785:Photons; D013997:Time Factors