Combined use of RNAi strategies with in vitro mouse limb bud culture.
Methods for knocking out genes and generating transgenic animals are available to study gene functions during embryogenesis. Recently, RNA interference (RNAi), a conserved eukaryotic mechanism in which double-stranded RNA induces sequence-specific degradation of homologous mRNAs, has become a powerful tool for investigating gene function in cells, whole animals, and embryos. Additionally, in vitro mouse limb bud culture is available to study limb development. By microinjection, we established a new method combining the use of RNAi strategies with in vitro mouse limb bud culture to investigate the functions of various genes during embryonic limb development. Using this new method, we found that HspB10 may play a role during skeletal development of limbs. It shows that the combined use of RNAi strategies (via microinjection) with in vitro mouse limb bud culture system may become a technique for investigating gene function in limb development.
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Peoples Republic of China.
Yongfei
Y
Zhu
Jiangbo
J
Zhu
Xuying
X
Wan
Yuping
Y
Zhu
Tianbao
T
Zhang
D000818:Animals; D001483:Base Sequence; D066298:In Vitro Techniques; D018878:Limb Buds / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
D035683:MicroRNAs