The incidence of ANA and ETI-dsDNA detected by enzyme immunoassays and indirect immunofluorescence assay (IFA).
While the SLE (Systemic Lupus Erythematosus) specificity of ANA is low, that of anti-dsDNA autoantibodies is high. The DNA used in the assay must be double stranded: autoantibodies to single-stranded (ss) DNA exist in many diseases and specific to none. The prevalence (70%) of anti-dsDNA autoantibodies is much higherin SLE, giving a higher diagnostic sensitivity than the similarly disease-specific anti-Sm autoantibodies (30%). Anti-dsDNA autoantibodies are usually detected by very analytically sensitive techniques, such as ELISA (Enzyme Linked Immunosorbent Assay). Within SLE, ds-DNA autoantibodies tend to associate with the presence of glomerulonephritis. Their levels are used to monitor disease activity. We suggest the use of ds-DNA to find the difference between SLE patientswith benign variants and classical syndrome of severe skin and renal disease.
Center for Genetics, Faculty of Medicine, University of Sarajevo, Cekalusa 80, 71000 Sarajevo, Bosnia and Herzegovina.
Izeta
I
Aganovic-Musinovic
Lamija
L
Prljaca-Zecevic
Djemo
D
Subasic
D000974:Antibodies, Antinuclear / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D015415:Biomarkers; D004247:DNA