The T1405N carbamoyl phosphate synthetase polymorphism does not affect plasma arginine concentrations in preterm infants.
BACKGROUND
A C-to-A nucleotide transversion (T1405N) in the gene that encodes carbamoyl-phosphate synthetase 1 (CPS1) has been associated with changes in plasma concentrations of L-arginine in term and near term infants but not in adults. In preterm infants homozygosity for the CPS1 Thr1405 variant (CC genotype) was associated with an increased risk of having necrotizing enterocolitis (NEC). Plasma L-arginine concentrations are decreased in preterm infants with NEC.


OBJECTIVE
To examine the putative association between the CPS1 T1405N polymorphism and plasma arginine concentrations in preterm infants.


METHODS
Prospective multicenter cohort study. Plasma and DNA samples were collected from 128 preterm infants (<30 weeks) between 6 and 12 hours after birth. Plasma amino acid and CPS1 T1405N polymorphism analysis were performed.


RESULTS
Distribution of genotypes did not differ between the preterm (CC:CA:AA = 55.5%:33.6%:10.9%, n = 128) and term infants (CC:CA:AA = 54.2%:35.4%:10.4%, n = 96). There was no association between the CPS1 genotype and plasma L-arginine or L-citrulline concentration, or the ornithine to citrulline ratio, which varies inversely with CPS1 activity. Also the levels of asymmetric dimethylarginine, and symmetric dimethylarginine were not significantly different among the three genotypes.


CONCLUSIONS
The present study in preterm infants did not confirm the earlier reported association between CPS1 genotype and L-arginine levels in term infants.
Moonen
Rob M J
RM
Department of Pediatrics, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.
Reyes
Iballa
I
Cavallaro
Giacomo
G
González-Luis
Gema
G
Bakker
Jaap A
JA
Villamor
Eduardo
E
D019943:Amino Acid Substitution / Q000235:genetics*; D001120:Arginine / Q000097:blood*; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006262:Health; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D047928:Premature Birth / Q000097:blood* / Q000235:genetics*; D014508:Urea / Q000378:metabolism
D014508:Urea; D001120:Arginine; D002222:Carbamoyl-Phosphate Synthase (Ammonia)