Acinetobacter baylyi starvation-induced genes identified through incubation in long-term stationary phase.
Acinetobacter species encounter cycles of feast and famine in nature. We show that populations of Acinetobacter baylyi strain ADP1 remain dynamic for 6 weeks in batch culture. We created a library of lacZ reporters inserted into SalI sites in the genome and then isolated 30 genes with lacZ insertions whose expression was induced by starvation during long-term stationary phase compared with their expression during exponential growth. The genes encode metabolic, gene expression, DNA maintenance, envelope, and conserved hypothetical proteins.
Department of Biology, Colorado College, Colorado Springs, Colorado 80903, USA.
C Phoebe
CP
Lostroh
Bruce A
BA
Voyles
D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D019677:Artificial Gene Fusion; D020869:Gene Expression Profiling / Q000379:methods; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D007763:Lac Operon; D013312:Stress, Physiological; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001616:beta-Galactosidase