Strategies for kinome profiling in cancer and potential clinical applications: chemical proteomics and array-based methods.
Kinases are key enzymes involved in deregulated signal transduction associated with cancer development and progression. The advent of personalized medicine drives the development of new diagnostic tools for patient stratification and therapy selection Ginsburg and Willard (Transl Res 154:277-287, 2009). Since deregulation of kinase-mediated signal transduction is implied in tumorigenesis, the analysis of all kinases (the kinome) active in a particular tumor may yield tumor-specific information on aberrant cell signalling pathways. Tumor tissue kinase activity profiles may correlate with response to therapy and therefore may be used for future therapy selection. In this Trend paper we describe peptide array and mass spectrometry-based technologies and new developments for kinome profiling, and we present an outlook towards future implementation of therapy selection based on kinome profiling in clinical practice.
Department of Medical Oncology, OncoProteomics Laboratory, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
Sander R
SR
Piersma
Mariette
M
Labots
Henk M W
HM
Verheul
Connie R
CR
Jiménez
D006801:Humans; D013058:Mass Spectrometry; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*
D010770:Phosphotransferases