Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis.
The acoABCL and acuABC operons of Bacillus licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the acoABCL operon is controlled by a sigmaL-dependent promoter and the acuABC operon by a sigmaA-dependent promoter. Transcription at the acoA promoter is repressed by glucose but induced by acetoin as soon as the preferred carbon source glucose is exhausted. The acuA promoter shows a similar induction pattern, but its activity is independent from the presence of acetoin. It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis.
Institut für Pharmazie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Trung Nguyen
TN
Thanh
Britta
B
Jürgen
Melanie
M
Bauch
Manuel
M
Liebeke
Michael
M
Lalk
Armin
A
Ehrenreich
Stefan
S
Evers
Karl-Heinz
KH
Maurer
Haike
H
Antelmann
Florian
F
Ernst
Georg
G
Homuth
Michael
M
Hecker
Thomas
T
Schweder
D000093:Acetoin / Q000378:metabolism*; D001407:Bacillus / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002072:Butylene Glycols / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic
D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000093:Acetoin