Use of the HCP5 single nucleotide polymorphism to predict hypersensitivity reactions to abacavir: correlation with HLA-B*5701.
OBJECTIVE
To study the correlation between the HLA-B*5701 allele and the single nucleotide polymorphism in HCP5 (rs2395029).


METHODS
All HIV patients naive for abacavir seen at our institution between September 2007 and December 2008 were prospectively screened for HLA-B*5701. HCP5 rs2395029 genotyping was carried out by allelic discrimination using the TaqMan 5'-nuclease assay. High-resolution HLA class I typing was undertaken using sequence-specific primers.


RESULTS
A total of 245 HIV patients were included in the study. A good correlation between HLA-B*5701 and HCP5 was observed (negative and positive predictive values of 100% and 93%, respectively).


CONCLUSIONS
The use of HCP5 rs2395029 testing could be as useful as HLA-B*5701 typing to prevent the abacavir hypersensitivity reaction. Given that HCP5 testing is cheaper, less time-consuming and easier to perform than HLA typing, it may confidently replace the latter in clinical settings.
Pharmacology and Pharmacogenetic Unit, Hospital Carlos III, Madrid, Spain. sonia_r_novoa@hotmail.com
Sonia
S
Rodríguez-Nóvoa
Lorena
L
Cuenca
Judit
J
Morello
Mateo
M
Córdoba
Francisco
F
Blanco
Inmaculada
I
Jiménez-Nácher
Vincent
V
Soriano
D000328:Adult; D019380:Anti-HIV Agents / Q000009:adverse effects*; D015224:Dideoxynucleosides / Q000009:adverse effects*; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D011446:Prospective Studies; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D000099227:Abacavir
D019380:Anti-HIV Agents; D015224:Dideoxynucleosides; D015235:HLA-B Antigens; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D000099227:Abacavir; C080521:HCP5 long noncoding RNA, human; C484830:HLA-B*57:01 antigen