Inhibitory effect of L-type voltage-dependent calcium channel blocker on response of urinary bladder with acute ethanol intoxication.
OBJECTIVE
This study was performed to assess the effect of L-type voltage-dependent calcium channel (VDCC) blocker (dilitiazem) on the response of the urinary bladder with ethanol intoxication in in vivo and in vitro studies.


METHODS
Sprague-Dawley rats were used for in vivo and in vitro studies. The strips were divided into 5 groups according to pretreatment. Group I-A was treated with ethanol (0.1%), group I-B with ethanol (0.5%), group II with diltiazem treatment (10(-6) M), group III-A with pretreatment of diltiazem (10(-6) M) with ethanol intoxication (0.1%) and group III-B with pretreatment of diltiazem with ethanol intoxication (0.5%). The carbachol-induced tension was compared before and after each pretreatment. In separate in vivo experiments, the changes of maximal vesical pressure and intercontraction interval after intra-arterial administration of each agent (identical grouping with in vitro study) were monitored.


RESULTS
The carbachol-induced contractions in group I-A, group I-B, group II, group III-A and group III-B were significantly decreased after each pretreatment (95 ± 2.73%, 92.6 ± 2.5%, 65.4 ± 2.0%, 52.61 ± 5.16%, 14.9 ± 1.4% of the control). The degree of increment of intercontraction interval and decrement of maximal vesical pressure showed a significant difference in the presence of diltiazem and ethanol intoxication (0.5%) compared with the diltiazem-treated and ethanol-intoxicated groups (0.5%).


CONCLUSIONS
There is a possibility that ethanol and L-type VDCC blockers have synergistic depressive effect on bladder contractility and that ethanol and L-type VDCC blockers act through a common ionic pathway.
Oh
Mi Mi
MM
Department of Urology, Korea University Hospital, Seoul, Korea.
Bae
Jae Hyun
JH
Kim
Je Jong
JJ
Kim
Hyung Jee
HJ
Moon
Du Geon
du G
Lee
Jeong Gu
JG
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020746:Calcium Channels, L-Type / Q000737:chemistry; D002217:Carbachol / Q000494:pharmacology; D004110:Diltiazem / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000187:drug effects* / Q000503:physiopathology
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D000431:Ethanol; D002217:Carbachol; D004110:Diltiazem