Staphylococcal catalase regulates its virulence and induces arthritis in catalase deficient mice.
To figure out whether in vivo expression of Staphylococcal catalase could correlate with the virulence and pathogenicity of the bacteria in the catalase deficient Swiss albino mice. 3 Amino 1, 2, 4 triazole (ATZ) (2 mg/g body wt) treated catalase deficient mice were infected with virulent S. aureus and bacterial burden, antioxidant enzyme levels were estimated after 3, 5 and 10 days of infection. Arthritic scores and levels of serum uric acid in mice were also determined. ATZ treatment was found to have slowed down the clearance of bacteria from blood and their rapid elimination from spleen. Increased tissue catalase activities in the spleen and liver of ATZ pre-treated mice even after 5 days of infection suggested its bacterial origin. It was further verified by zymographic analysis. Increased swelling of joints was observed after 5 days of infection. Uric acid level was found lesser in ATZ treated mice. ATZ treatment slowed the bacterial passage from blood with a lower tissue anti-oxidant enzymes leading to induction of joint inflammation.
Immunology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata - 700 009.
Riti
R
Sen
Debaditya
D
Das
Biswadev
B
Bishayi
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D001170:Arthritis, Infectious / Q000209:etiology* / Q000235:genetics* / Q000473:pathology; D002374:Catalase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology*; D015169:Colony Count, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007202:Indicators and Reagents; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000097:blood / Q000382:microbiology; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000472:pathogenicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014527:Uric Acid / Q000097:blood
D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D014527:Uric Acid; D002374:Catalase; D013482:Superoxide Dismutase; D000640:Amitrole