[Purification of oleuropein by sephadex LH-20 column].
OBJECTIVE
To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph.


METHODS
This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC.


RESULTS
The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL.


CONCLUSIONS
Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.
Dang
Jian-Zhang
JZ
Applied Biological Department of Shenzhen Polytechnic College, Shenzhen 518055, China. djzmail@sina.com
Nie
Xiao-Zhong
XZ
Liang
Li-Qin
LQ
D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003911:Dextrans; D000431:Ethanol / Q000737:chemistry; D057888:Iridoid Glucosides; D039823:Iridoids; D031658:Olea / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*
D003911:Dextrans; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C002769:oleuropein; D000431:Ethanol; C025614:sephadex