Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa KüHN.
Dwarf bunt of wheat, caused by Tilletia controversa KüHN: , is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 microg of teliospores in a 25-microL PCR reaction mixture.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing, 100193, P.R. China.
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L
Gao
W Q
WQ
Chen
T G
TG
Liu
D001483:Base Sequence; D001487:Basidiomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D014908:Triticum / Q000382:microbiology*
D017931:DNA Primers; D004271:DNA, Fungal