Ferrous chelator 2,2'-dipyridyl attenuates cerebral vasospasm after experimental subarachnoid haemorrhage in rabbits.
The effect of 2,2'-dipyridyl (DP) on cerebral vasospasm was investigated in a double-injection rabbit model of subarachnoid haemorrhage (SAH). Thirty-six animals were divided between four groups: control (sham-operated), SAH (model alone), SAH + DP (the SAH model in which DP dissolved in dimethyl sulphoxide [DMSO] was injected once daily for 5 days into the cisterna magna), and SAH + DMSO (the SAH model in which DMSO [vehicle] was injected daily for 5 days). There were significant differences in the basilar artery luminal area, wall thickness, neurological deficit score and vasospasm index between the SAH + DP and SAH groups. There was a significant negative correlation between arterial luminal area and arterial wall thickness, and also between the neurological deficit score and vasospasm index. Cells that were positive for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labelling (TUNEL) and p53 expression were significantly increased in the SAH + DMSO and SAH groups, but not in the SAH + DP group, versus controls. Thus, DP may attenuate cerebral vasospasm after SAH by suppressing p53-induced apoptosis in the cerebral vessels.
Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Y Y
YY
Yu
L
L
Niu
L
L
Gao
G L
GL
Zhang
J
J
Li
J P
JP
Deng
Y Z
YZ
Qu
Z W
ZW
Zhao
G D
GD
Gao
D015082:2,2'-Dipyridyl / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002614:Chelating Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D013345:Subarachnoid Hemorrhage / Q000188:drug therapy*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D020301:Vasospasm, Intracranial / Q000517:prevention & control*
D002614:Chelating Agents; D016159:Tumor Suppressor Protein p53; D015082:2,2'-Dipyridyl