Chimeric antibodies against tick-borne encephalitis virus.
Two chimeric antibodies (ch) 13D6 and 10C2 against the glycoprotein E of tick-borne encephalitis virus (TBEV) were constructed by fusing variable regions of murine monoclonal antibodies (Mabs) 13D6 and 10C2 to human constant regions. Monovalent analogues of these antibodies in format of single-chain antibodies (scFv or sc) were developed, as well. The ch13D6, ch10C2, sc13D6 and sc10C2 exhibited binding characteristics similar to parental Mabs. Only the ch13D6 and sc13D6 were able to neutralize TBEV infectivity in vitro. The in vitro neutralization provided by ch13D6 suggests that this antibody can be further developed into a potent prophylaxis and therapy for tick-borne encephalitis (TBE) infection.
Federal State Research Institution State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region 630559, Russia. levanov.lev@gmail.com
Lev N
LN
Levanov
Leonid E
LE
Matveev
Elena P
EP
Goncharova
Leonid R
LR
Lebedev
Alexander B
AB
Ryzhikov
Tatyana E
TE
Yun
Tatyana A
TA
Batanova
Alexander N
AN
Shvalov
Ivan K
IK
Baykov
Lyudmila N
LN
Shingarova
Michael P
MP
Kirpichnikov
Nina V
NV
Tikunova
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus