Identification of the residues in the extracellular domain of thrombopoietin receptor involved in the binding of thrombopoietin and a nuclear distribution protein (human NUDC).
Thrombopoietin (TPO) and its receptor (Mpl) have long been associated with megakaryocyte proliferation, differentiation, and platelet formation. However, studies have also shown that the extracellular domain of Mpl (Mpl-EC) interacts with human (h) NUDC, a protein previously characterized as a human homolog of a fungal nuclear migration protein. This study was undertaken to further delineate the putative binding domain on the Mpl receptor. Using the yeast two-hybrid system assay and co-immunoprecipitation, we identified that within the Mpl-EC domain 1 (Mpl-EC-D1), amino acids 102-251 were strongly involved in ligand binding. We subsequently expressed five subdomains within this region with T7 phage display. Enzyme-linked immunosorbent binding assays identified a short stretch of peptide located between residues 206 and 251 as the minimum binding domain for both TPO and hNUDC. A series of sequential Ala replacement mutations in the region were subsequently used to identify the specific residues most involved in ligand binding. Our results point to two hydrophobic residues, Leu(228) and Leu(230), as having substantial effects on hNUDC binding. For TPO binding, mutations in residues Asp(235) and Leu(239) had the largest effect on binding efficacy. In addition, deletion of the conservative motif WGSWS reduced binding capacity for hNUDC but not for TPO. These separate binding sites on the Mpl receptor for TPO and hNUDC raise interesting implications for the cytokine-receptor interactions.
Chen
Wei-Min
WM
Key Laboratory of Gene Engineering of Education Ministry, Zhongshan University, Guangzhou 510275, China.
Yu
Bo
B
Zhang
Qing
Q
Xu
Peilin
P
D000596:Amino Acids; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism*; D017124:Conserved Sequence; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D053628:Receptors, Thrombopoietin / Q000737:chemistry / Q000378:metabolism*; D013926:Thrombopoietin / Q000737:chemistry / Q000378:metabolism*
D000596:Amino Acids; D018797:Cell Cycle Proteins; C512648:NUDC protein, human; D009687:Nuclear Proteins; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin