Generation of mice with a conditional null allele of the Jagged2 gene.
The Notch signaling pathway is an evolutionarily-conserved intercellular signaling mechanism, and mutations in its components disrupt embryonic development in many organisms and cause inherited diseases in humans. The Jagged2 (Jag2) gene, which encodes a ligand for Notch pathway receptors, is required for craniofacial, limb, and T cell development. Mice homozygous for a Jag2 null allele die at birth from cleft palate, precluding study of Jag2 function in postnatal and adult mice. We have generated a Jag2 conditional null allele by flanking the first two exons of the Jag2 gene with loxP sites. Cre-mediated deletion of the Jag2(flox) allele generates the Jag2(del2) allele, which behaves genetically as a Jag2 null allele. This Jag2 conditional null allele will enable investigation of Jag2 function in a variety of tissue-specific contexts.
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Jingxia
J
Xu
Luke T
LT
Krebs
Thomas
T
Gridley
D000818:Animals; D015139:Blotting, Southern; D002972:Cleft Palate / Q000235:genetics*; D019465:Craniofacial Abnormalities / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005804:Genes, Lethal; D006720:Homozygote; D017403:In Situ Hybridization; D019426:Integrases / Q000378:metabolism; D000072101:Jagged-2 Protein; D017880:Limb Deformities, Congenital / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype
C490503:Jag2 protein, mouse; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; C045073:Cre recombinase; D019426:Integrases