Low-carbohydrate and high-fat diets on the promotion of hepatic steatosis in rats.
OBJECTIVE
The present work looked for to evaluate in rats the impact of different diets (high-lipid and high-lipid+high-protein) on liver, verifying the occurrence of oxidative stress and steatosis.


METHODS
The animals were treated with the respective diets (Group HLS: high-lipid diet with 50% of saturated fat; Group HPLS: high-lipid and high-protein diet with 50% of saturated fat and 40% of protein; Group Control: control diet AIN-93) for 28 days. After this period the animals were sacrificed for hepatic determinations of MDA, reduced GSH, vitamin E, steatosis and glycemia.


RESULTS
The results showed higher glycemia in the group HPLS, high concentration of MDA and GSH in the group Control and decreased hepatic vitamin E concentration in the groups that received the high-lipid diets. The hepatic fat was higher in the groups HPLS and HLS in relation to the Group Control, however HPLS presenting high level of fat concentration, showing similar results as the steatosis.


CONCLUSIONS
the fat increase in the diet promoted increase of the oxidative stress, evidenced by the decrease in the hepatic concentration of vitamin E, showing its antioxidant role against the probable generated free radicals, the ones which possibly exercised a role in the steatosis occurrence.
Leonardi
D S
DS
Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brasil. dsleonardi@gmail.com
Feres
M B de C
MB
Portari
G V
GV
Zanuto
M E
ME
Zucoloto
S
S
Jordão
A A
AA
D000818:Animals; D001835:Body Weight; D002364:Caseins / Q000009:adverse effects; D050528:Diet, Carbohydrate-Restricted / Q000009:adverse effects*; D004040:Dietary Carbohydrates / Q000009:adverse effects; D004041:Dietary Fats / Q000009:adverse effects*; D004044:Dietary Proteins / Q000009:adverse effects*; D005234:Fatty Liver / Q000097:blood / Q000453:epidemiology* / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006943:Hyperglycemia / Q000453:epidemiology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index; D014810:Vitamin E / Q000378:metabolism
D002364:Caseins; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004044:Dietary Proteins; D014810:Vitamin E; D008315:Malondialdehyde; D005978:Glutathione