Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated beta-amino acid.
Subtulene A, a new cyclic lipopeptide, was isolated from the culture broth of Bacillus subtilis SSE4. This antibiotic compound contained the seven common alpha-amino acids, L-Asn-1, D-Tyr-2, D-Asn-3, L-Gln-4, L-Pro-5, D-Asn-6, L-Ser-7 and the unique beta-amino acid-8 present in the iturin family. 1D and 2D NMR, as well as MS analyses, identified the beta-amino acid as 3-amino-13-methyltetradec-8-enoic acid, an Iso C15 long chain beta-amino acid. B. subtilis SSE4 was also found to produce iturin A. B. subtilis SSE4 culture filtrate exhibited both antifungal and antibacterial activities.
Thasana
Nopporn
N
Chemical Biology Program, Chulabhorn Graduate Institute, Bangkok, Thailand.
Prapagdee
Benjaphorn
B
Rangkadilok
Nuchanart
N
Sallabhan
Ratiboot
R
Aye
Seaim Lwin
SL
Ruchirawat
Somsak
S
Loprasert
Suvit
S
D000272:Adipates; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects; D008055:Lipids; D055666:Lipopeptides; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic; D012694:Serine / Q000031:analogs & derivatives; D014443:Tyrosine / Q000378:metabolism
D000272:Adipates; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D008055:Lipids; D055666:Lipopeptides; D010456:Peptides, Cyclic; C069989:serine containing aminolipid; D014443:Tyrosine; D012694:Serine; C013579:iturin A; D001216:Asparagine; C029900:adipic acid