Immunohistochemical expression of vascular endothelial growth factor (VEGF) in different types of odontogenic cysts.
The objective of the present study was to evaluate vascular endothelial growth factor (VEGF) expression in different types of odontogenic cysts. A total of 25 parakeratotic odontogenic keratocysts (POKCs), 16 orthokeratotic odontogenic keratocysts (OOKCs), and 28 follicular cysts (FCs) were evaluated semiquantitatively for immunohistochemical analysis of VEGF in epithelial cells, endothelial cells of blood vessels, inflammatory cells and focally stromal cells. A significant different expression of VEGF in all cell components was found in keratocysts compared to FCs. The POKCs (80%) and OOKCs (68%) showed more than 50% VEGF positive epithelial cells, whereas the majority of FCs (71%) were either negative in the epithelium or showed less than 10% positive cells. Similarly, the POKCs (88%) and OOKCs (68%) showed more than 50% positive endothelial cells, whereas the FCs (75%) were either negative or showed less than 10% VEGF positive endothelial cells. The highest percentage of cases with score 2 positivity in the stromal cells was observed in POKCs (68%); OOKCs showed a score 2 positivity in 44%, score 1 in 31% and score 0 in 25%, whereas 68% of FCs showed a score 0, 25% a score 1 and only 7% of cases showed a score 2. No statistically significant differences were observed between POKCs and OOKCs in VEGF expression in the epithelial and endothelial cells, whereas the positivity score in stromal cells was significantly higher in POKCs compared to OOKCs. The present results can support the hypothesis that angiogenesis is an active mechanism in the invasive growth of the OKC.
Rubini
Corrado
C
Department of Neurosciences, Pathologic Anatomy and Histopathology, Polytechnic University of Marche, Ancona, Italy. c.rubini@univpm.it
Artese
Luciano
L
Zizzi
Antonio
A
Fioroni
Massimiliano
M
Ascani
Giuliano
G
Goteri
Gaia
G
Stramazzotti
Daniela
D
Piccirilli
Marcello
M
Iezzi
Giovanna
G
Piattelli
Adriano
A
D000293:Adolescent; D000328:Adult; D001485:Basement Membrane / Q000473:pathology; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005497:Follicular Cyst / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D007571:Jaw Diseases / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology; D009807:Odontogenic Cysts / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D055815:Young Adult
C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A