title: Expression of a novel tropomyosin isoform in axolotl heart and skeletal muscle.
abstract: TPM1kappa is an alternatively spliced isoform of the TPM1 gene whose specific role in cardiac development and disease is yet to be elucidated. Although mRNA studies have shown TPM1kappa expression in axolotl heart and skeletal muscle, it has not been quantified. Also the presence of TPM1kappa protein in axolotl heart and skeletal muscle has not been demonstrated. In this study, we quantified TPM1kappa mRNA expression in axolotl heart and skeletal muscle. Using a newly developed TPM1kappa specific antibody, we demonstrated the expression and incorporation of TPM1kappa protein in myofibrils of axolotl heart and skeletal muscle. The results support the potential role of TPM1kappa in myofibrillogenesis and sarcomeric function.
authors.affiliation: Department of Medicine, SUNY Upstate Medical University, Syracuse, New York 13210, USA.
authors.forename: Anish
authors.initials: A
authors.lastname: Thomas
authors.forename: Sudarsan
authors.initials: S
authors.lastname: Rajan
authors.forename: Harold L
authors.initials: HL
authors.lastname: Thurston
authors.forename: Sreeharsha N
authors.initials: SN
authors.lastname: Masineni
authors.forename: Preeti
authors.initials: P
authors.lastname: Dube
authors.forename: Abhishek
authors.initials: A
authors.lastname: Bose
authors.forename: Vasundhara
authors.initials: V
authors.lastname: Muthu
authors.forename: Syamalima
authors.initials: S
authors.lastname: Dube
authors.forename: David F
authors.initials: DF
authors.lastname: Wieczorek
authors.forename: Bernard J
authors.initials: BJ
authors.lastname: Poiesz
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Dube
mesh_terms: D017398:Alternative Splicing; D000558:Ambystoma mexicanum; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D016612:Paraffin Embedding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D020033:Protein Isoforms; D012333:RNA, Messenger; D014335:Tropomyosin