title: Comprehensive maternal serum proteomic profiles of preclinical and clinical preeclampsia.
abstract: We systematically characterized maternal serum proteome in women with clinical preeclampsia (PE) and asymptomatic women in early pregnancy that subsequently developed PE. Clinical PE cohort comprised 30 patients with mild PE, 30 with severe PE, and 58 normotensive women. Preclinical PE cohort included 149 women whose serum samples were collected at 8-14 gestational weeks and in whom 30 women later developed mild and 40 severe PE. Serum proteome was analyzed and enzyme-linked immunosorbent assays were used for protein quantification. In Clinical PE, fibronectin, pappalysin-2, choriogonadotropin-beta, apolipoprotein C-III, cystatin-C, vascular endothelial growth factor receptor-1, and endoglin were more abundant compared to normotensive women. In preclinical PE, differently expressed proteins included placental, vascular, transport, matrix, and acute phase proteins. Angiogenic and antiangiogenic proteins were not significant. We conclude that placental and antiangiogenic proteins are abundant in clinical PE. In preclinical PE, proteomic profile is distinct and different from that in clinical PE.
authors.affiliation: Departments of Obstetrics and Gynecology, Pediatrics, Public Health and Preventive Medicine, Oregon Health & Science University, Portland, Oregon 97239, USA. juha.rasanen@oulu.fi
authors.forename: Juha
authors.initials: J
authors.lastname: Rasanen
authors.forename: Anna
authors.initials: A
authors.lastname: Girsen
authors.forename: Xinfang
authors.initials: X
authors.lastname: Lu
authors.forename: Jodi A
authors.initials: JA
authors.lastname: Lapidus
authors.forename: Melissa
authors.initials: M
authors.lastname: Standley
authors.forename: Ashok
authors.initials: A
authors.lastname: Reddy
authors.forename: Surendra
authors.initials: S
authors.lastname: Dasari
authors.forename: Archana
authors.initials: A
authors.lastname: Thomas
authors.forename: Thomas
authors.initials: T
authors.lastname: Jacob
authors.forename: Anneli
authors.initials: A
authors.lastname: Pouta
authors.forename: Helja-Marja
authors.initials: HM
authors.lastname: Surcel
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Tolosa
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gravett
authors.forename: Srinivasa R
authors.initials: SR
authors.lastname: Nagalla
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016015:Logistic Models; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins