Genomic identification of a novel mutation in hfq that provides multiple benefits in evolving glucose-limited populations of Escherichia coli.
Beneficial mutations in diversifying glucose-limited Escherichia coli populations are mostly unidentified. The genome of an evolved isolate with multiple differences from that of the ancestor was fully assembled. Remarkably, a single mutation in hfq was responsible for the multiple benefits under glucose limitation through changes in at least five regulation targets.
Maharjan
Ram
R
School of Molecular Bioscience, University of Sydney, New South Wales, Australia.
Zhou
Zhemin
Z
Ren
Yan
Y
Li
Yang
Y
Gaffé
Joël
J
Schneider
Dominique
D
McKenzie
Christopher
C
Reeves
Peter R
PR
Ferenci
Thomas
T
Wang
Lei
L
D000222:Adaptation, Physiological; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology*; D035001:Host Factor 1 Protein / Q000235:genetics* / Q000378:metabolism; D009154:Mutation
D029968:Escherichia coli Proteins; C087373:Hfq protein, E coli; D035001:Host Factor 1 Protein; D005947:Glucose