title: High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro.
abstract: OBJECTIVE
  To study the effect of FSH on the aneuploidy risk of human oocytes matured in vitro.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Hospital-based IVF center.
  
  
  METHODS
  Patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles.
  
  
  METHODS
  Immature oocytes were put into five groups according to the FSH concentration (0, 5.5, 22, 100, and 2,000 ng/mL) in in vitro maturation (IVM) medium. Spindles were observed under a polarized microscope before polar body biopsy. Fixed polar bodies and corresponding oocytes were examined on chromosomes 13, 16, 18, 21, and 22 by fluorescence in situ hybridization. Oocytes matured in 5.5 and 2,000 ng/mL FSH were immunostained for tubulin and chromatin.
  
  
  METHODS
  Aneuploidy rate, spindle visualization rate, and spindle morphology.
  
  
  RESULTS
  The frequency rates of aneuploidy were 26.7%, 23.3%, 36.75%, 46.67%, and 63.3% in the five FSH groups, respectively. There was a significantly higher aneuploidy rate in oocytes matured in the 2,000 ng/mL FSH group. The spindle visualization rates assessed under PolScope were not significantly different between aneuploid and normal oocytes. There was no difference in spindle morphology between the 2,000 and 5.5 ng/mL FSH groups.
  
  
  CONCLUSIONS
  High-concentration FSH in IVM medium significantly increased the first meiotic division error, resulting in more aneuploid oocytes during IVM.
authors.affiliation: Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Xu
authors.forename: Yue-Ting
authors.initials: YT
authors.lastname: Peng
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zeng
authors.forename: Guang-Lun
authors.initials: GL
authors.lastname: Zhuang
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Zhou
mesh_terms: D000782:Aneuploidy; D018929:Cell Culture Techniques; D020090:Chromosome Segregation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000009:adverse effects*; D006801:Humans; D008859:Microscopy, Polarization; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D012307:Risk Factors; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000187:drug effects
chemical_list: D005640:Follicle Stimulating Hormone