Production of recombinant human lysozyme in the milk of transgenic pigs.
In the swine industry pathogenic infections have a significant negative impact on neonatal survival. Piglets fed with human lysozyme, a natural antibiotic, might be more resistant to gastrointestinal infections. Here we describe the generation of transgenic swine expressing recombinant human lysozyme by somatic cell nuclear transfer. Three cloned female pigs were born, one of which expressed rhLZ at 0.32 ± 0.01 μg/ml in milk, 50-fold higher than that of the pig native lysozyme. Both the transgenic gilts and their progeny appear healthy. Introducing human lysozyme into pigs' milk has a potential to benefit the piglets by enhancing immune function and defending against pathogenic bacteria, thereby increasing the new born survival rate. This advance could be of great value to commercial swine producers.
Tong
Jia
J
State Key Laboratory for Agrobiotechnology, China Agricultural University, 100193, Beijing, China.
Wei
HengXi
H
Liu
XiaoFang
X
Hu
WenPing
W
Bi
MingJun
M
Wang
YuanYuan
Y
Li
QiuYan
Q
Li
Ning
N
D000822:Animal Husbandry / Q000379:methods; D000818:Animals; D030801:Animals, Genetically Modified; D019976:Cloning, Organism; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism; D008892:Milk / Q000201:enzymology*; D009113:Muramidase / Q000096:biosynthesis* / Q000235:genetics; D053652:Nuclear Transfer Techniques; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019076:Transgenes
D011994:Recombinant Proteins; D009113:Muramidase