Acquisition of a transposon encoding extended-spectrum beta-lactamase SHV-12 by Pseudomonas aeruginosa isolates during the clinical course of a burn patient.
Three of seven clonally related Pseudomonas aeruginosa strains isolated from a burn patient produced the extended-spectrum beta-lactamase (ESBL) SHV-12. Its gene was flanked by two IS26 elements with a large transposon (>24 kb). The transposon also contained at least five IS26 elements and a gene encoding the amikacin resistance determinant aminoglycoside 6'-N-acetyltransferase type Ib [aac(6')-Ib]. It was inserted into the gene PA5317 in the P. aeruginosa chromosome.
Uemura
Shuji
S
Department of Traumatology and Critical Care Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan. uemura@sapmed.ac.jp
Yokota
Shin-ichi
S
Mizuno
Hirotoshi
H
Sakawaki
Eiji
E
Sawamoto
Keigo
K
Maekawa
Kunihiko
K
Tanno
Katsutoshi
K
Mori
Kazuhisa
K
Asai
Yasufumi
Y
Fujii
Nobuhiro
N
D000328:Adult; D002056:Burns / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases