title: Modifications of band 3 and oxidation level of membrane proteins in senescent erythrocytes.
abstract: OBJECTIVE
  To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN ANDMETHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups.
  
  
  RESULTS
  Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC.
  
  
  CONCLUSIONS
  These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.
authors.affiliation: Facultad de Ciencias Bioquímicas y Farmacéuticas. Universidad Nacional de Rosario, Argentina.
authors.forename: Angel
authors.initials: A
authors.lastname: Rucci
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Ensinck
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mufarrege
authors.forename: Carlos
authors.initials: C
authors.lastname: Cotorruelo
authors.forename: Silvia García
authors.initials: SG
authors.lastname: Borrás
authors.forename: Liliana
authors.initials: L
authors.lastname: Racca
authors.forename: Claudia
authors.initials: C
authors.lastname: Biondi
authors.forename: Amelia
authors.initials: A
authors.lastname: Racca
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins