title: Laser nephelometry applied in an automated microplate system to study filamentous fungus growth.
abstract: By contrast with photometry (i.e., the measurement of light transmitted through a particle suspension), nephelometry is a direct method of measuring light scattered by particles in suspension. Since the scattered light intensity is directly proportional to the suspended particle concentration, nephelometry is a promising method for recording microbial growth and especially for studying filamentous fungi, which cannot be efficiently investigated through spectrophotometric assays. We describe herein for the first time a filamentous fungi-tailored procedure based on microscale liquid cultivation and automated nephelometric recording of growth, followed by extraction of relevant variables (lag time and growth rate) from the obtained growth curves. This microplate reader technique is applicable for the evaluation of antifungal activity and for large-scale phenotypic profiling.
authors.affiliation: IFR 149 QUASAV, UMR PaVé A77, Angers, France.
authors.forename: Aymeric
authors.initials: A
authors.lastname: Joubert
authors.forename: Benoît
authors.initials: B
authors.lastname: Calmes
authors.forename: Romain
authors.initials: R
authors.lastname: Berruyer
authors.forename: Marc
authors.initials: M
authors.lastname: Pihet
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Bouchara
authors.forename: Philippe
authors.initials: P
authors.lastname: Simoneau
authors.forename: Thomas
authors.initials: T
authors.lastname: Guillemette
mesh_terms: D000528:Alternaria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D000935:Antifungal Agents / Q000494:pharmacology; D001331:Automation / Q000295:instrumentation*; D015169:Colony Count, Microbial; D004149:Dioxoles / Q000494:pharmacology; D005800:Genes, Fungal / Q000235:genetics; D005990:Glycerol / Q000494:pharmacology; D007834:Lasers; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D009994:Osmolar Concentration; D011758:Pyrroles / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D004149:Dioxoles; D011758:Pyrroles; C108339:fludioxonil; D005990:Glycerol