title: Preferential localization of IgG memory B cells adjacent to contracted germinal centers.
abstract: It has long been presumed that after leaving the germinal centers (GCs), memory B cells colonize the marginal zone or join the recirculating pool. Here we demonstrate the preferential localization of nitrophenol-chicken gamma-globulin-induced CD38(+)IgG1(+) memory B cells adjacent to contracted GCs in the spleen. The memory B cells in this region proliferated after secondary immunization, a response that was abolished by depletion of CD4(+) T cells. We also found that these IgG1(+) memory B cells could present antigen on their surface, and that this activity was required for their activation. These results implicate this peri-GC region as an important site for survival and reactivation of memory B cells.
authors.affiliation: Laboratory for Lymphocyte Differentiation, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Aiba
authors.forename: Kohei
authors.initials: K
authors.lastname: Kometani
authors.forename: Megumi
authors.initials: M
authors.lastname: Hamadate
authors.forename: Saya
authors.initials: S
authors.lastname: Moriyama
authors.forename: Asako
authors.initials: A
authors.lastname: Sakaue-Sawano
authors.forename: Michio
authors.initials: M
authors.lastname: Tomura
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Hans Jörg
authors.initials: HJ
authors.lastname: Fehling
authors.forename: Rafael
authors.initials: R
authors.lastname: Casellas
authors.forename: Osami
authors.initials: O
authors.lastname: Kanagawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kurosaki
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000276:immunology / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002645:Chickens; D005434:Flow Cytometry; D018858:Germinal Center / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007150:Immunohistochemistry; D007156:Immunologic Memory / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009596:Nitrophenols / Q000737:chemistry; D010648:Phenylacetates / Q000737:chemistry; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005719:gamma-Globulins / Q000737:chemistry / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D009596:Nitrophenols; D010648:Phenylacetates; D005719:gamma-Globulins; C026242:4-hydroxy-5-nitrophenyl acetic acid; D051997:ADP-ribosyl Cyclase 1