Fura-2FF-based calcium indicator for protein labeling.
We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.
Ruggiu
Agostina A
AA
Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
Bannwarth
Michael
M
Johnsson
Kai
K
D002118:Calcium / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D016257:Fura-2 / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006147:Guanine / Q000737:chemistry; D011506:Proteins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D011506:Proteins; D006147:Guanine; D002118:Calcium; D016257:Fura-2