Donor strand exchange and conformational changes during E. coli fimbrial formation.
Fimbriae and pili are macromolecular structures on the surface of Gram negative bacteria that are important for cellular adhesion. A 2.7Å resolution crystal structure of a complex of Escherichia coli fimbrial proteins containing FimH, FimG, FimF, and FimC provides the most complete model to date for the arrangement of subunits assembled in the native structure. The first three proteins form the tip of the fimbriae while FimC is the chaperone protein involved in the usher/chaperone assembly process. The subunits interact through donor strand complementation where a β-strand from a subunit completes the β-sandwich structure of the neighboring subunit or domain closer to the tip of the fimbria. The function of FimC is to provide a surrogate donor strand before delivery of each subunit to the FimD usher and the growing fimbria. Comparison of the subunits in this structure and their chaperone-bound complexes show that the two FimH domains change their relative orientation and position in forming the tip structure. Also, the non-chaperone subunits undergo a conformational change in their first β-strand when the chaperone is replaced by the native donor strand. Some residues move as much as 14Å in the process. This structural shift has not been noted in structural studies of other bacterial adhesion sub-structures assembled via donor strand complementation. The domains undergo a significant structural change in the donor strand binding groove during fimbrial assembly, and this likely plays a role in determining the specificity of subunit-subunit interactions among the fimbrial proteins.
Le Trong
Isolde
I
Department of Biological Structure, University of Washington, Box 357420, Seattle, WA 98195-7420, USA.
Aprikian
Pavel
P
Kidd
Brian A
BA
Thomas
Wendy E
WE
Sokurenko
Evgeni V
EV
Stenkamp
Ronald E
RE
D018830:Adhesins, Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary
D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; C521674:FimF protein, E coli; C529458:FimG protein, E coli; C489721:fimC protein, E coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins