Alterations in the developing testis transcriptome following embryonic vinclozolin exposure.
The current study investigates the direct effects of in utero vinclozolin exposure on the developing F1 generation rat testis transcriptome. Previous studies have demonstrated that exposure to vinclozolin during embryonic gonadal sex determination induces epigenetic modifications of the germ line and transgenerational adult onset disease states. Microarray analyses were performed to compare control and vinclozolin treated testis transcriptomes at embryonic days 13, 14 and 16. A total of 576 differentially expressed genes were identified and the major cellular functions and pathways associated with these altered transcripts were examined. The sets of regulated genes at the different development periods were found to be transiently altered and distinct. Categorization by major known functions of altered genes was performed. Specific cellular process and pathway analyses suggest the involvement of Wnt and calcium signaling, vascular development and epigenetic mechanisms as potential mediators of the direct F1 generation actions of vinclozolin.
Clement
Tracy M
TM
Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, USA.
Savenkova
Marina I
MI
Settles
Matthew
M
Anway
Matthew D
MD
Skinner
Michael K
MK
D000818:Animals; D047108:Embryonic Development / Q000187:drug effects* / Q000235:genetics; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D005865:Gestational Age; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010080:Oxazoles / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism / Q000473:pathology
D052244:Endocrine Disruptors; D010080:Oxazoles; C025643:vinclozolin