title: Improving computational protein design by using structure-derived sequence profile.
abstract: Designing a protein sequence that will fold into a predefined structure is of both practical and fundamental interest. Many successful, computational designs in the last decade resulted from improved understanding of hydrophobic and polar interactions between side chains of amino acid residues in stabilizing protein tertiary structures. However, the coupling between main-chain backbone structure and local sequence has yet to be fully addressed. Here, we attempt to account for such coupling by using a sequence profile derived from the sequences of five residue fragments in a fragment library that are structurally matched to the five-residue segments contained in a target structure. We further introduced a term to reduce low complexity regions of designed sequences. These two terms together with optimized reference states for amino-acid residues were implemented in the RosettaDesign program. The new method, called RosettaDesign-SR, makes a 12% increase (from 34 to 46%) in fraction of proteins whose designed sequences are more than 35% identical to wild-type sequences. Meanwhile, it reduces 8% (from 22% to 14%) to the number of designed sequences that are not homologous to any known protein sequences according to psi-blast. More importantly, the sequences designed by RosettaDesign-SR have 2-3% more polar residues at the surface and core regions of proteins and these surface and core polar residues have about 4% higher sequence identity to wild-type sequences than by RosettaDesign. Thus, the proteins designed by RosettaDesign-SR should be less likely to aggregate and more likely to have unique structures due to more specific polar interactions.
authors.affiliation: School of Informatics, Indiana University Purdue University, Indianapolis, Indiana 46202, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Dai
authors.forename: Yuedong
authors.initials: Y
authors.lastname: Yang
authors.forename: Hyung Rae
authors.initials: HR
authors.lastname: Kim
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008970:Molecular Weight; D010446:Peptide Fragments; D011487:Protein Conformation; D017510:Protein Folding; D055550:Protein Stability; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D012984:Software; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D011506:Proteins