title: Design, synthesis, and biological evaluation of resveratrol analogues as aromatase and quinone reductase 2 inhibitors for chemoprevention of cancer.
abstract: A series of new resveratrol analogues were designed and synthesized and their inhibitory activities against aromatase were evaluated. The crystal structure of human aromatase (PDB 3eqm) was used to rationalize the mechanism of action of the aromatase inhibitor 32 (IC50 0.59 microM) through docking, molecular mechanics energy minimization, and computer graphics molecular modeling, and the information was utilized to design several very potent inhibitors, including compounds 82 (IC50 70 nM) and 84 (IC50 36 nM). The aromatase inhibitory activities of these compounds are much more potent than that for the lead compound resveratrol, which has an IC50 of 80 microM. In addition to aromatase inhibitory activity, compounds 32 and 44 also displayed potent QR2 inhibitory activity (IC50 1.7 microM and 0.27 microM, respectively) and the high-resolution X-ray structures of QR2 in complex with these two compounds provide insight into their mechanism of QR2 inhibition. The aromatase and quinone reductase inhibitors resulting from these studies have potential value in the treatment and prevention of cancer.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmaceutical Sciences, and The Purdue Center for Cancer Research, Purdue University, West Lafayette, IN 47907, United States.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Juma
authors.initials: J
authors.lastname: Hoshino
authors.forename: Katie
authors.initials: K
authors.lastname: Jermihov
authors.forename: Laura
authors.initials: L
authors.lastname: Marler
authors.forename: John M
authors.initials: JM
authors.lastname: Pezzuto
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Mesecar
authors.forename: Mark
authors.initials: M
authors.lastname: Cushman
mesh_terms: D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D001141:Aromatase / Q000737:chemistry / Q000378:metabolism*; D047072:Aromatase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D018890:Chemoprevention; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D009369:Neoplasms / Q000517:prevention & control*; D011808:Quinone Reductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D014703:Veratrum / Q000737:chemistry
chemical_list: D016588:Anticarcinogenic Agents; D047072:Aromatase Inhibitors; D013267:Stilbenes; D001141:Aromatase; C419642:NRH - quinone oxidoreductase2; D011808:Quinone Reductases; D000077185:Resveratrol