title: First outbreak of PVL-positive nonmultiresistant MRSA in a neonatal ICU in Australia: comparison of MALDI-TOF and SNP-plus-binary gene typing.
abstract: The purpose of this brief report is to describe the first outbreak of a community-associated nonmultiresistant and PVL-positive MRSA strain (CC30) in a neonatal intensive care unit in Australia. The utility of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) for microbial typing is compared with single nucleotide polymorphism (SNP) plus binary gene analysis. The composite correlation index analysis of the MALDI-TOF-MS data demonstrated the similar inter-strain relatedness found with the SNP-plus-binary gene typing used to confirm the outbreak. The evolving spread of MRSA emphasizes the importance of surveillance, infection control vigilance and the ongoing investigation of rapid typing methods for MRSA.
authors.affiliation: Microbiology Department (Level 5), Pathology Queensland Central Laboratory (Block 7), Herston Hospitals Campus, Herston, Brisbane 4029, Queensland, Australia. sanmarie_schlebusch@health.qld.gov.au
authors.forename: S
authors.initials: S
authors.lastname: Schlebusch
authors.forename: G R
authors.initials: GR
authors.lastname: Price
authors.forename: S
authors.initials: S
authors.lastname: Hinds
authors.forename: C
authors.initials: C
authors.lastname: Nourse
authors.forename: J M
authors.initials: JM
authors.lastname: Schooneveldt
authors.forename: M H
authors.initials: MH
authors.lastname: Tilse
authors.forename: H G
authors.initials: HG
authors.lastname: Liley
authors.forename: T
authors.initials: T
authors.lastname: Wallis
authors.forename: F
authors.initials: F
authors.lastname: Bowling
authors.forename: D
authors.initials: D
authors.lastname: Venter
authors.forename: G R
authors.initials: GR
authors.lastname: Nimmo
mesh_terms: D001315:Australia / Q000453:epidemiology; D001427:Bacterial Toxins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques / Q000379:methods*; D017714:Community-Acquired Infections / Q000453:epidemiology* / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005098:Exotoxins / Q000096:biosynthesis*; D005838:Genotype; D006801:Humans; D007223:Infant; D007363:Intensive Care Units, Neonatal; D007956:Leukocidins / Q000096:biosynthesis*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D017720:Molecular Epidemiology / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin