Recombinant N-terminal part of bovine herpesvirus 1 ICP27 protein: its preparation, purification, and use for raising specific antiserum.
Recombinant N-terminal part of bovine herpesvirus-1 (BoHV-1) ICP27 protein fused with thioredoxin and His-tag ("the recombinant protein") expressed in Escherichia coli was purified by the Ni2+-NTA affinity chromatography and used for the preparation of antiserum by immunization of rabbits. The antiserum recognized the recombinant protein in Western blot analysis and was able to detect BoHV-1 ICP27 in the nucleoli of BoHV-1-infected MDBK cells. These results showed that such an antiserum could serve as a valuable tool in further studies of the functions of BoHV-1 ICP27.


BACKGROUND
Bovine herpesvirus 1, ICP27, recombinant protein, antiserum, immunofluorescence assay.
Zhao
L
L
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences Wuhan 430071, PR, China.
Ren
X
X
Guo
H
H
Ding
Q
Q
Zheng
A C
AC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004285:Dogs; D007242:Herpesvirus 1, Bovine / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins