Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes.
Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to 2-deoxyribose 5-phosphate with a molar yield of 55%.
Honda
Kohsuke
K
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. honda@bio.eng.osaka-u.ac.jp
Maya
Shohei
S
Omasa
Takeshi
T
Hirota
Ryuichi
R
Kuroda
Akio
A
Ohtake
Hisao
H
D032701:Biomimetics / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D005240:Feasibility Studies; D005632:Fructose / Q000737:chemistry*; D005818:Genetic Engineering / Q000379:methods*; D008190:Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012267:Ribosemonophosphates / Q000138:chemical synthesis*; D015398:Signal Transduction / Q000502:physiology; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D012267:Ribosemonophosphates; D005632:Fructose; C058245:2-deoxyribose 5-phosphate; D008190:Lyases