title: [Influence of cross-protection on the survival of Lactobacillus casei ATCC 393].
abstract: OBJECTIVE
  In this study, we investigated the cross-protection of Lactobacillus casei ATCC 393 under multi-stress conditions.
  
  
  METHODS
  Cells pre-adapted to mild conditions (heat, H2O2, acid or bile salts) were then treated at lethal temperature (> 60 degrees C) or hydrogen peroxide stress (> 5 mmol/L). Furthermore, the changes of survival rate intracellular pH and membrane fatty acid under lethal conditions with or without acid adaption were compared.
  
  
  RESULTS
  The cross-protection in Lactobacillus casei ATCC 393 were affected by different stress conditions. Acid pre-adaption, especially hydrochloride treatment, would increase the resistance of cells to lethal heat and peroxide stresses significantly, with the survival rate of 305-fold and 173-fold, respectively. Further study suggested that the effect of acid pre-adaption might be related to the regulation on intracellular pH and the saturation of cell membrane.
  
  
  CONCLUSIONS
  Hydrochloride adaption was the best inducer for the cross-protection of Lactobacillus casei ATCC 393 to maintain relatively stable physiological status of cells. The results supplied a novel way to investigate the relationship between different protective mechanisms in L. casei under different kinds of stresses.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. tcxuefeng@126.com
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology; D056738:Cross Protection / Q000187:drug effects* / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D013696:Temperature
chemical_list: D001647:Bile Acids and Salts; D006861:Hydrogen Peroxide