title: Novel florfenicol and chloramphenicol resistance gene discovered in Alaskan soil by using functional metagenomics.
abstract: Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
authors.forename: Kevin S
authors.initials: KS
authors.lastname: Lang
authors.forename: Janet M
authors.initials: JM
authors.lastname: Anderson
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.forename: Lynn
authors.initials: L
authors.lastname: Williamson
authors.forename: Jo
authors.initials: J
authors.lastname: Handelsman
authors.forename: Randall S
authors.initials: RS
authors.lastname: Singer
mesh_terms: D000413:Alaska; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002702:Chloramphenicol Resistance; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D056186:Metagenomics / Q000379:methods*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C035534:florfenicol; D013839:Thiamphenicol