Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure.
Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
Tanaka
Torahiko
T
Department of Advanced Medical Science, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi, Tokyo 173-8610, Japan. tanakat@med.nihon-u.ac.jp
Hasegawa
Yuichiro
Y
Saito
Makoto
M
Ikeda
Masanori
M
Kato
Nobuyuki
N
D000941:Antigens / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D057127:Single-Chain Antibodies / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D012995:Solubility
D000941:Antigens; D003902:Detergents; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies