title: Direct detection of methicillin-resistant Staphylococcus aureus from blood cultures using an immunochromatographic immunoassay-based MRSA rapid kit for the detection of penicillin-binding protein 2a.
abstract: Using an EZ-Step MRSA rapid kit, a novel screening test for methicillin-resistant Staphylococcus aureus (MRSA) that detects penicillin-binding protein 2a, 34 of 36 MRSA-positive clinical blood culture samples were positive on direct testing (sensitivity, 94.4%), whereas 21 of 21 methicillin-susceptible S. aureus-positive samples were negative (specificity, 100%).
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju 361-711, South Korea. ksshin@chungbuk.ac.kr [corrected]
authors.forename: Kyeong Seob
authors.initials: KS
authors.lastname: Shin
authors.forename: Hyung Geun
authors.initials: HG
authors.lastname: Song
authors.forename: Haejung
authors.initials: H
authors.lastname: Kim
authors.forename: Sangsun
authors.initials: S
authors.lastname: Yoon
authors.forename: Seung Bok
authors.initials: SB
authors.lastname: Hong
authors.forename: Sun Hoe
authors.initials: SH
authors.lastname: Koo
authors.forename: Jimyung
authors.initials: J
authors.lastname: Kim
authors.forename: Jongwan
authors.initials: J
authors.lastname: Kim
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Roh
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001426:Bacterial Proteins / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D046915:Penicillin-Binding Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins