title: Fresh-frozen bone allografts in maxillary ridge augmentation: histologic analysis.
abstract: Bone allograft has become an alternative to autogenous bone due to its decreased operative trauma and the almost unlimited supply of reconstructive material. The aim of the present study was to histologically evaluate the suitability of fresh-frozen bone graft (test group) used in maxillary ridge augmentation, comparing it to autogenous bone (native maxilla: control group). During the re-entry procedures, 9 months after the fresh-frozen allogeneic bone blocks were placed in the atrophic maxillary ridges, bone cores were removed with a trephine bur from test and control treatments in the same patient. Routine histologic processing using hematoxylin and eosin and Picrosirius staining was performed. Mature and immature collagen area and density analysis were carried out for both groups under polarization. The results of Student's t test for paired samples (P > .05) showed no statistically significant difference in mature and immature collagen area or density percentage between test and control groups. Histologically similar bone formation patterns were observed in both groups. We concluded that fresh-frozen bone allograft is a biologically acceptable alternative for augmentation of the deficient alveolar ridge, showing a similar collagen pattern to that of autogenous bone.
authors.affiliation: School of Dentistry, Universidade Tuiuti do Paraná, Brazil. cintiacontar@hotmail.com
authors.forename: Cintia Mussi Milani
authors.initials: CM
authors.lastname: Contar
authors.forename: João Rodrigo
authors.initials: JR
authors.lastname: Sarot
authors.forename: Maite Barroso
authors.initials: MB
authors.lastname: da Costa
authors.forename: Jayme
authors.initials: J
authors.lastname: Bordini
authors.forename: Antonio Adilson Soares
authors.initials: AA
authors.lastname: de Lima
authors.forename: Luciana Reis Azevedo
authors.initials: LR
authors.lastname: Alanis
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: Trevilatto
authors.forename: Maria Ângela Naval
authors.initials: MÂ
authors.lastname: Machado
mesh_terms: D000328:Adult; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D015519:Bone Density; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen / Q000737:chemistry; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D008437:Maxilla / Q000601:surgery*; D008875:Middle Aged; D014183:Transplantation, Heterologous
chemical_list: D003094:Collagen