title: Effect of chronic intermittent hypoxia on biological behavior and hypoxia-associated gene expression in lung cancer cells.
abstract: Hypoxia plays an important role in the development of solid tumors and is associated with their therapeutic resistance. There exist three major forms of hypoxia: acute, chronic, and intermittent hypoxia. Previous studies have shown that cancer cells could behave in the form of adaptation to hypoxia in tumor growth, which could result in their biological changes and determine their responses to the therapies. To investigate the tumor cells' adaptation to hypoxia, we recreated two models using two lung cancer cell lines in the presence of intermittent hypoxia, which is characterized by changes in oxygen pressure within the disorganized vascular network. We investigated biological behaviors such as cell cycle, proliferation, radiation sensitivity, apoptosis and migration, hypoxia signal pathway in the lung cancer cells treated with chronic intermittent hypoxia, as well as the role of hypoxia inducible factor 1 there, hypoxia-inducible genes analyzed by real-time RT-PCR chip in H446 cells treated with the model. The results indicated the changes of some hypoxia target gene expressions of those induced by hypoxia, some of which were confirmed by real-time RT-PCR. The cells mediated by irradiation induced resistance to radiation and apoptosis and increased metastasis in lung cancer cells. It was found that such changes were related to hypoxia inducible factor 1, alpha subunit (HIF-1α).
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan, Shandong Province, PR China.
authors.forename: Yonglei
authors.initials: Y
authors.lastname: Liu
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
authors.forename: Xingwu
authors.initials: X
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Xiuju
authors.initials: X
authors.lastname: Liu
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Yuan
authors.forename: Liyan
authors.initials: L
authors.lastname: Lv
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D000860:Hypoxia / Q000235:genetics*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008175:Lung Neoplasms / Q000473:pathology*; D009362:Neoplasm Metastasis
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit