[Change of hydrogen sulfide content in serum of rats after cardiopulmonary resuscitation].
OBJECTIVE
To investigate variation of hydrogen sulfide (H2S) in serum of rats after cardiopulmonary resuscitation (CPR) and explore its pathophysiological role in CPR.


METHODS
The 30 male SD rats were randomly divided into control group (n=6) and experimental group (n=24), then models of cardiac arrest (CA) were established in rats of experimental group by transcutaneous electrical epicardium stimulation. Blood samples were collected before CA, at 2, 4, 6, 8, 10 and 12 h after restoration of spontaneous circulation (ROSC) for testing the serum levels of H2S, at the same time rectal temperature (Tr), mean arterial pressure (MAP), heart rate (HR) and respiration rate (R) were recorded. Next to analyze the dynamic variation of H2S content in serum of the rats after CPR and its correlation of the above-mentioned signs of life.


RESULTS
(1) In experimental group, all of the 24 rats were successfully induced CA and resuscitation; at end points (12 h after ROSC) there were 14 rats alive and their vital signs were stable. There was no death in the control group. (2) The variation trend of H2S content in serum was different between experimental group and the control (F=12.226, P=0.003). In experimental group, H2S content in serum increased dramatically after CPR and reached peak at 6 h after ROSC, then return to the similar level of the control group. (3) H2S content in serum was negatively correlated with Tr (partial r=-0.556, P=0.000) and MAP (partial r=-0.240, P=0.002). But it wasn't correlated with HR and R (P>0.05).


CONCLUSIONS
Change of H2S content in serum of rats after CPR may be a manifestation of compensatory response after ROSC and participate in physiological processes such as body temperature and blood pressure regulation. But as for its precisely mechanisms, it still needs to be studied further.
Lin
Ji-yan
JY
Department of Emergency, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. doctorlinjiyan@yahoo.com
Wei
Hong-yan
HY
Li
Hui
H
Li
Xin
X
Liu
Rong
R
Hu
Chun-lin
CL
Huang
Guo-qing
GQ
Dai
Gang
G
Liao
Xiao-xing
XX
D000818:Animals; D001794:Blood Pressure; D001831:Body Temperature; D016887:Cardiopulmonary Resuscitation; D006339:Heart Rate; D006862:Hydrogen Sulfide / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D056152:Respiratory Rate; D013997:Time Factors
D006862:Hydrogen Sulfide