title: Effect of cytoskeleton inhibitors on conidiogenesis and capsule in the long neck yeast Fellomyces examined by scanning electron microscopy.
abstract: BACKGROUND
  The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans.
  
  
  METHODS
  Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy.
  
  
  RESULTS
  During conidiogenesis, mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared.
  
  
  CONCLUSIONS
  Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, UCB, Brno, Czech Republic. mkopecka@med.muni.cz
authors.forename: Marie
authors.initials: M
authors.lastname: Kopecká
authors.forename: Ladislav
authors.initials: L
authors.lastname: Ilkovics
authors.forename: Vladimíra
authors.initials: V
authors.lastname: Ramíková
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D001487:Basidiomycota / Q000187:drug effects* / Q000648:ultrastructure*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D048430:Cell Shape / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D013172:Spores, Fungal / Q000187:drug effects* / Q000648:ultrastructure*; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D053778:Thiazolidines; C037067:latrunculin A