title: Preparation of selenium/zinc-enriched probiotics and their effect on blood selenium and zinc concentrations, antioxidant capacities, and intestinal microflora in canine.
abstract: The aim of this study was to prepare Se/Zn-enriched probiotics and investigate their effect on blood Se and Zn concentrations, blood antioxidant capacities, and intestinal microflora in canine. The preparation was performed in a single-factor experiment. The optimal culture conditions were as follows: the initial concentrations of Se(4+) and Zn(2+) were 5 and 150 µg/ml, respectively; the inoculation volume was 5%; and the liquid volume of the medium was 50 ml in a 250-ml flask. The medium was then cultured at 32°C for 36 h. The biomass of the final product was 26.83 g/l, organic Se concentration was 173.35 µg/g, organic Zn concentration was 4.38 mg/g, Candida utilis biomass was 8.69 lg colony-forming units (CFU)/ml, and Lactobacillus biomass was 9.61 lg CFU/ml. In vivo, 20 indigenous dogs were randomly assigned to two dietary treatment groups for a 35-day study and fed a basal diet or a diet supplemented with 2.0 g of Se/Zn-enriched probiotics. Blood and fecal samples were collected on days 0, 15, and 30 of the experiment. Compared with the control group, the blood Se concentration; the blood Zn concentration; the activities of glutathione peroxidase, superoxide dismutase, and total antioxidant capacity in the blood; and the amount of Lactobacillus and Bifidobacterium in the feces increased in the supplemented group during the period of supplementation (P < 0.01), while malondialdehyde level in the blood and the amounts of Escherichia coli, Staphylococcus, and Enterococcus in the feces decreased in the supplemented group (P < 0.01).
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yangguang
authors.initials: Y
authors.lastname: Wang
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004285:Dogs / Q000097:blood*; D004334:Drug Administration Schedule; D005260:Female; D008297:Male; D019936:Probiotics / Q000737:chemistry / Q000494:pharmacology*; D012643:Selenium / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D012643:Selenium; D015032:Zinc