[Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology].
OBJECTIVE
To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.


METHODS
siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.


RESULTS
1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.


CONCLUSIONS
siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.
Jia
Haiying
H
Department of Otolaryngology Head and Neck Surgery, First Affiliated Hopsital, Ji'nan University, Guangzhou, China. jiahaiying79@126.com
Zhang
Tao
T
Tang
Zhi
Z
Wang
Jiqun
J
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D018390:Gene Targeting; D005822:Genetic Vectors; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D019098:Telomerase / Q000235:genetics*; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012313:RNA; C523666:hTERT mp30-38 peptide; D019098:Telomerase