title: Rationally improving LOV domain-based photoswitches.
abstract: Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Devin
authors.initials: D
authors.lastname: Strickland
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yao
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Gawlak
authors.forename: Michael K
authors.initials: MK
authors.lastname: Rosen
authors.forename: Kevin H
authors.initials: KH
authors.lastname: Gardner
authors.forename: Tobin R
authors.initials: TR
authors.lastname: Sosnick
mesh_terms: D018554:Avena; D008027:Light; D010777:Photochemistry / Q000379:methods*; D056949:Phototropins / Q000737:chemistry* / Q000528:radiation effects; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000528:radiation effects
chemical_list: D056949:Phototropins; D011506:Proteins