Lack of association between transient receptor potential cation channel 6 polymorphisms and primary membranous glomerulonephritis.
Membranous glomerulonephritis (MGN) is one common cause of idiopathic nephrotic syndrome. Transient receptor potential cation channel 6 (TRPC6) has been identified as causing a familial form of progressive focal and segmental glomerulosclerosis. The objective was to clarify the relationship between TRPC6 polymorphisms and MGN. We recruited a cohort of 134 biopsy-diagnosed MGN patients and 265 healthy subjects. Genotyping of TRPC6 polymorphisms was performed using allele-specific polymerase chain reaction methods. We then analyzed associations between TRPC6 gene polymorphisms and clinical manifestations and pathogenesis of MGN. There was no statistically significant difference of TRPC6 gene rs3824935 C/T, rs17096918 C/T, and rs4326755 A/G polymorphisms between controls and patients with MGN. There was no statistical significance of allele frequencies in these two groups. The characteristics of clinical parameters in TRPC6 gene (rs3284935) C/T polymorphism revealed no difference except proteinuria (p < 0.0005) between CC and non-CC genotype in MGN patients. Besides, no apparent statistically significant differences of rs17096918 C/T (TT and non-TT) and rs4326755 A/G (AA and non-AA) polymorphisms between genotypes were found in the clinical parameters. There is no different genotype distribution between normal controls and patients with MGN of TRPC6 gene. The data also show that TRPC6 gene may not be associated with disease clinical course of MGN.
Chen
Wen-Chi
WC
Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan.
Chen
Shih-Yin
SY
Chen
Cheng-Hsu
CH
Chen
Huey-Yi
HY
Lin
Yi-Wen
YW
Ho
Tsung-Jung
TJ
Huang
Yu-Chuen
YC
Shen
Jui-Lung
JL
Tsai
Fuu-Jen
FJ
Chen
Yung-Hsiang
YH
D005260:Female; D015433:Glomerulonephritis, Membranous / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D050052:TRPC Cation Channels / Q000235:genetics*; D000074287:TRPC6 Cation Channel
D050052:TRPC Cation Channels; D000074287:TRPC6 Cation Channel; C117032:TRPC6 protein, human