Sequencing protocols to genotype mdx, mdx(4cv), and mdx(5cv) mice.
Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives.
Banks
Glen B
GB
Department of Neurology, University of Washington, Mail Stop 357720, 1959 NE Pacific St., Seattle, Washington 98195, USA.
Combs
Ariana C
AC
Chamberlain
Jeffrey S
JS
D000818:Animals; D016189:Dystrophin / Q000235:genetics*; D005189:False Positive Reactions; D005838:Genotype; D051379:Mice; D018101:Mice, Inbred mdx / Q000235:genetics*; D009137:Muscular Dystrophy, Animal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D016189:Dystrophin