Noradrenaline-induced spontaneous inhibitory postsynaptic currents in mouse basolateral nucleus of amygdala pyramidal neurons: comparison with dopamine-induced currents.
The basolateral nucleus of the amygdala (BLA) receives both noradrenergic and dopaminergic projections. These projections are thought to be important for modulation of amygdala neural circuits. In BLA pyramidal neurons, noradrenaline (NA) is known to facilitate gamma-aminobutyric acid (GABA)ergic spontaneous inhibitory postsynaptic currents (sIPSCs) through excitation of interneurons. Dopamine (DA) also is known to facilitate GABAergic sIPSCs in pyramidal neurons of the amygdala region including the BLA. It is unclear which neurotransmitter, NA or DA, is predominant in facilitating sIPSC in the BLA. Whether NA and DA facilitate sIPSC in different or the same pyramidal neurons also remains unknown. Herein, we employed the patch clamp recording technique on BLA pyramidal neurons in mouse brain slices, and compared the facilitating actions of NA and DA on sIPSCs. First NA and then DA, or first DA and then NA, were applied to a slice. NA enhanced sIPSC frequency in the majority (80-90%) of pyramidal neurons tested, whereas DA enhanced sIPSC frequency in relatively few neurons (approximately 30%). Neurons responding to NA alone and DA alone accounted, respectively, for 54.3% and 2.9% of the pyramidal neurons tested (11.4% of neurons responded to neither NA nor DA). Pyramidal neurons in which NA and DA both facilitated sIPSCs accounted for 31.4% of neurons tested. These results suggest that NA facilitates GABAergic sIPSCs in a larger proportion of mouse BLA pyramidal neurons than DA.
Miyajima
Moeko
M
Department of Anesthesiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Ozaki
Makoto
M
Wada
Keiji
K
Sekiguchi
Masayuki
M
D000679:Amygdala / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004298:Dopamine / Q000494:pharmacology / Q000502:physiology*; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology / Q000502:physiology*; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D005680:gamma-Aminobutyric Acid; D004298:Dopamine; D009638:Norepinephrine