title: Molecular differentiation and phylogenetic relationships of three Angiostrongylus species and Angiostrongylus cantonensis geographical isolates based on a 66-kDa protein gene of A. cantonensis (Nematoda: Angiostrongylidae).
abstract: The phylogenetic relationships and molecular differentiation of three species of angiostrongylid nematodes (Angiostrongylus cantonensis, Angiostrongylus costaricensis and Angiostrongylus malaysiensis) were studied using the AC primers for a 66-kDa protein gene of A. cantonensis. The AC primers successfully amplified the genomic DNA of these angiostrongylid nematodes. No amplification was detected for the DNA of Ascaris lumbricoides, Ascaris suum, Anisakis simplex, Gnathostoma spinigerum, Toxocara canis, and Trichinella spiralis. The maximum-parsimony (MP) consensus tree and the maximum-likelihood (ML) tree both showed that the Angiostrongylus taxa could be divided into two major clades - Clade 1 (A. costaricensis) and Clade 2 (A. cantonensis and A. malaysiensis) with a full support bootstrap value. A. costaricensis is the most distant taxon. A. cantonensis is a sister group to A. malaysiensis; these two taxa (species) are clearly separated. There is no clear distinction between the A. cantonensis samples from four different geographical localities (Thailand, China, Japan and Hawaii); only some of the samples are grouped ranging from no support or low support to moderate support of bootstrap values. The published nucleotide sequences of A. cantonensis adult-specific native 66kDa protein mRNA, clone L5-400 from Taiwan (U17585) appear to be very distant from the A. cantonensis samples from Thailand, China, Japan and Hawaii, with the uncorrected p-distance values ranging from 26.87% to 29.92%.
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. sipes@mahidol.ac.th
authors.forename: Praphathip
authors.initials: P
authors.lastname: Eamsobhana
authors.forename: Phaik Eem
authors.initials: PE
authors.lastname: Lim
authors.forename: Hongman
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Gan
authors.forename: Hoi Sen
authors.initials: HS
authors.lastname: Yong
mesh_terms: D000801:Angiostrongylus / Q000145:classification* / Q000235:genetics; D017185:Angiostrongylus cantonensis / Q000145:classification / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001700:Biomphalaria; D002681:China; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006254:Hawaii; D015801:Helminth Proteins / Q000235:genetics*; D007564:Japan; D016013:Likelihood Functions; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D051381:Rats; D016415:Sequence Alignment; D013785:Thailand
chemical_list: D018509:DNA, Helminth; D015801:Helminth Proteins