A basic study on the biological monitoring for vanadium-effects of vanadium on Vero cells and the evaluation of intracellular vanadium contents.
A high concentration of vanadium (V) has toxic effects on human and animals and is one of environmental pollutants. In the present study, we have conducted a fundamental study using cultured Vero cells from monkey kidney for the future environmental monitoring. Orthovanadate (VAN), one of V compounds, of 10(-10) and 10(-8) M did not affect the cell growth although the higher concentration of above 10(-6) M VAN inhibited the cell growth accompanied with the decrease in cell numbers and morphological changes. Given that the washing method with ice-cold Li is also effective for determination of the cellular Na content, we used this method for the determination of the V content of the Vero cells. The V distributions in Vero cell; in the 10(-3) M VAN solution, extracellular and intracellular were obtained as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min after the treatment of VAN. The intracellular V content was 10% of the applied concentration of VAN. Consequently, it was suggested that V concentration of 10(-7) and 10(-6) M in the tissue and environment, respectively, might become the threshold concentration; a criterion of the environmental contamination when we carry out environmental monitoring.
Mochizuki
Mariko
M
Department of Applied Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Kyounan, Musashino, Tokyo, Japan.
Kudo
Eiko
E
Kikuchi
Mitsuho
M
Takano
Takashi
T
Taniuchi
Yojiro
Y
Kitamura
Tomoya
T
Hondo
Ryo
R
Ueda
Fukiko
F
D000818:Animals; D049108:Cell Enlargement; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D008094:Lithium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D018675:Toxicity Tests; D014639:Vanadium / Q000378:metabolism / Q000633:toxicity*; D014709:Vero Cells
D004785:Environmental Pollutants; D014639:Vanadium; D008094:Lithium; D012964:Sodium