An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.
Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.
Liu
Yunbao
Y
Bioactive Natural Products and Phytoceuticals, Department of Horticulture and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.
Nair
Muraleedharan G
MG
D000975:Antioxidants / Q000191:economics / Q000378:metabolism / Q000494:pharmacology*; D004396:Coloring Agents / Q000191:economics / Q000494:pharmacology; D005562:Formazans; D016166:Free Radical Scavengers / Q000191:economics / Q000378:metabolism / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D015394:Molecular Structure; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000191:economics / Q000378:metabolism / Q000494:pharmacology*; D026082:Singlet Oxygen / Q000737:chemistry; D013778:Tetrazolium Salts / Q000191:economics; D013844:Thiazoles / Q000191:economics
D000975:Antioxidants; D004396:Coloring Agents; D005562:Formazans; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; D026082:Singlet Oxygen; D009249:NADP; C022616:thiazolyl blue