Halostagnicola alkaliphila sp. nov., an alkaliphilic haloarchaeon from commercial rock salt.
A Gram-negative, pleomorphic, aerobic, haloalkaliphilic archaeon, strain 167-74(T), was isolated from commercial rock salt imported into Japan from China. Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 167-74(T) is closely related to Halostagnicola larsenii XH-48(T) (98.3 %) and Halostagnicola kamekurae 194-10(T) (97.2 %). The major polar lipids of the isolate were C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. A glycolipid was not detected, in contrast to the two existing, neutrophilic species of the genus Halostagnicola. The DNA G+C content of strain 167-74(T) was 60.7 mol%. and it gave DNA-DNA reassociation values of 19.5 and 18.8 %, respectively, with Hst. larsenii JCM 13463(T) and Hst. kamekurae 194-10(T). Therefore, strain 167-74(T) represents a novel species, for which the name Halostagnicola alkaliphila sp. nov. is proposed, with the type strain 167-74(T) ( = JCM 16592(T)  = CECT 7631(T)).
Nagaoka
Shuhei
S
Department of Biological Applied Chemistry, Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Minegishi
Hiroaki
H
Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Echigo
Akinobu
A
Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Shimane
Yasuhiro
Y
Graduate School of Interdisciplinary New Science, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Kamekura
Masahiro
M
Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba 278-0043, Japan.
Usami
Ron
R
Graduate School of Interdisciplinary New Science, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.|Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
D000468:Alkalies / Q000378:metabolism*; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*
D000468:Alkalies; D019641:DNA, Archaeal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride