[Expression of IL-8, SP-A and TGF-beta1 in bronchoalveolar lavage fluid of neonates with bronchopulmonary dysplasia].
OBJECTIVE
To investigate the expression of interleukin 8 (IL-8), surfactant protein-A (SP-A) and transforming growth factor beta1 (TGF-beta1) in bronchoalveolar lavage fluid (BALF) of neonates with bronchopulmonary dysplasia (BPD).


METHODS
Thirty neonates with BPD and 30 gestational age-, gender-, and birth weight-matched neonates without BPD (control group) were enrolled from December 2007 to October 2009. Non-brochoscopic bronchoalveolar lavage was performed. The levels of IL-8, SP-A and TGF-beta1 in BALF were measured using ELISA.


RESULTS
The levels of TGF beta1 (47+/-15 microg/mL vs 34+/-13 microg/mL) and IL-8 (54+/-16 microg/mL vs 28+/-13 microg/mL) in the BPD group were significantly higher than those in the control group (P<0.01). In contrast, the contents of SP-A in the BPD group were significantly lower than those in the control group (35+/-16 microg/mL vs 42+/-14 microg/mL;P<0.05).


CONCLUSIONS
The increased expression of TGF-beta1 and IL-8 in BALF may be involved in abnormal lung development and maturation in neonates with BPD. The low expression of SP-A in the BPD group suggests that the exogenous SP-A administration may be an option for the treatment of BPD.
Liu
Dong-Yun
DY
Bayi Children's Hospital Affiliated to Beijing Military Region General Hospital, Beijing 100700, China.
Wu
Jing
J
Zhang
Xiao-Ying
XY
Feng
Zhi-Chun
ZC
D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001997:Bronchopulmonary Dysplasia / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D016209:Interleukin-8 / Q000032:analysis* / Q000502:physiology; D008297:Male; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis* / Q000502:physiology; D053773:Transforming Growth Factor beta1 / Q000032:analysis* / Q000502:physiology
D016209:Interleukin-8; D037662:Pulmonary Surfactant-Associated Protein A; D053773:Transforming Growth Factor beta1