Proteomics analysis of MPP+-induced apoptosis in SH-SY5Y cells.
Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP(+)-treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP(+)-induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson's disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.
Xie
Hongrong
H
Department of Neurology, The First Hospital, Jilin University, 71 Xinmin Street, Changchun, 130021, People's Republic of China.
Chang
Ming
M
Hu
Xinyu
X
Wang
Danping
D
Tian
Mingxiu
M
Li
Guoyi
G
Jiang
Huiyi
H
Wang
Ying
Y
Dong
Zhong
Z
Zhang
Yuhua
Y
Hu
Linsen
L
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020267:MPTP Poisoning / Q000378:metabolism* / Q000473:pathology; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009498:Neurotoxins / Q000633:toxicity*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
D009498:Neurotoxins; D015655:1-Methyl-4-phenylpyridinium