title: An approach towards peptide-based antibody detection for diagnosis of Chikungunya infection.
abstract: Chikungunya infection, although rarely fatal, is associated with significant morbidity which necessitates its diagnosis in the initial stages. Currently for diagnosis, together with clinical symptoms, immunological methods such as IgG/IgM detection, molecular methods such as real-time reverse transcriptase-polymerase chain reaction and viral isolation methods are available but they are either not very specific or they require high-level sophisticated infrastructures. In the present study, an enzyme-linked immunosorbent assay to evaluate antibody responses to peptides designed from the CHIKV E2 envelope glycoprotein was performed. Synthesized peptides were evaluated with confirmed Chikungunya and non-Chikungunya serum samples for antibody detection. The results demonstrate that the synthetic peptide-based diagnosis of Chikungunya can be an efficient and a more accessible approach in immunodiagnostics.
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Bajaj Nagar, Nagpur, India.
authors.forename: Shweta H
authors.initials: SH
authors.lastname: Morey
authors.forename: Rajpal S
authors.initials: RS
authors.lastname: Kashyap
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
authors.forename: Girdhar M
authors.initials: GM
authors.lastname: Taori
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis* / Q000276:immunology*; D000595:Amino Acid Sequence; D000906:Antibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002646:Chikungunya virus; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007159:Immunologic Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology* / Q000302:isolation & purification; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D010455:Peptides; D014764:Viral Proteins