Nucleotide sequences of four segments of chrysovirus in Korean Cryphonectria nitschkei BS122 strain.
The near full-length genome consisting of four segments of dsRNA from a chrysovirus infecting Korean Cryphonectria nitschkei BS122 strain (CnV1-BS122) was sequenced. The open reading frames of segments 1, 2, 3, and 4 were 2,889, 2,721, 2,475, and 2,232 nucleotides (nt) in length, respectively. Sequence analysis and homology searches of the amino acid sequences deduced from the ORFs of each segment revealed that segments 1, 2, 3, and 4 encoded RNA-dependent RNA polymerase, capsid protein, a putative cysteine protease, and replication-associated protein, respectively. The entire 5' ends of segments 1, 2, and 4 were 82, 242, and 698 nt in length, respectively; the sequence of the 5' end of segment 3 was not determined because of difficulty in amplification. The entire 3' end of segment 3 was 77 nt in length. Partial amplification of the 3' ends of segments 1, 2, and 4 yielded amplimers of 7, 17, and 30 nt, respectively.
Kim
Jung-Mi
JM
Division of Biological Sciences, Institute of Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Chonbuk, Korea.
Park
Jin-Ah
JA
Park
Seung-Moon
SM
Cha
Byeong-Jin
BJ
Yang
Moon-Sik
MS
Kim
Dae-Hyuk
DH
D001203:Ascomycota / Q000821:virology*; D001483:Base Sequence; D016679:Genome, Viral; D007723:Korea; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins