[Two types of MWNTs with different surface modifications induce differential expression of proteins in RAW264.7 cells].
OBJECTIVE
To compare the different expression of protein in RAW264.7 macrophage cells induced by two types of MWNTs (multi-walled carbon nanotubes) with different surface modifications (acid-treated MWNTs and tau-MWNTs modified by taurine).


METHODS
Treating cells with both types of MWNTs in 20 mg/L and 24 h, with a blank-control group set. Cells are lysed by using urea and by ultrasonicating in ice bath, then total proteins of cells are extracted. Using two-dimensional gel electrophoresis to separate total proteins of cells, searching for the differential expressed protein spots on the images of the gels with silver staining. Identifying the differentially expressed proteins via mass spectrometry, and studying the mechanism of effects on cells imposed by two types of MWNTs at protein level.


RESULTS
There are 13 spots of protein with notably differential expression among three treated groups (including blank controls). Their functions involve apoptosis-related, calcium-binding, cell-cycle related, DNA synthesis, folding of proteins, and energy metabolism, etc. The results are consistent with our previous studies about the cytotoxicity of Both types of MWNTs including induction of apoptosis and mitochodira damage.


CONCLUSIONS
Both two types of MWNTs could induce alteration of protein expression in RAW264.7 cells. With different surface modifications, they imposed different effects. High throughout proteomics could be applied in toxicity assessment and mechanism investigation about carbon nanotubes.
Shen
Zhen-lin
ZL
Department of Occupational and Environmental Health, Peking University School of Public Health, Beijing 100191, China.
Nie
Hai-yu
HY
Wang
Hai-fang
HF
Yang
Bin
B
Zhong
Li-jun
LJ
Zou
Xia-juan
XJ
Lou
Ya-xin
YX
Liu
Dan
D
Guo
Jian
J
Jia
Guang
G
D000143:Acids / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013499:Surface Properties; D013654:Taurine / Q000737:chemistry
D000143:Acids; D037742:Nanotubes, Carbon; D011506:Proteins; D020543:Proteome; D013654:Taurine