Biofilm formation and genotyping of Candida haemulonii, Candida pseudohaemulonii, and a proposed new species (Candida auris) isolates from Korea.
Emergence of Candida haemulonii and closely related species at five Korean hospitals has been recently described. We examined biofilm formation by these isolates and assessed their genotypic relatedness by pulsed-field gel electrophoresis (PFGE). This study is the first to show that all bloodstream isolates of Candida pseudohaemulonii can form significant biofilms in glucose-containing medium. PFGE of NotI-digested genomic DNA revealed that C. pseudohaemulonii isolates recovered from seven patients in two hospitals shared five patterns, and that 15 isolates of a proposed new species (Candida auris) obtained from patients at three hospitals shared seven patterns, suggesting that some of these isolates may be related to clonal transmission.
Oh
Bong Joon
BJ
Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
Shin
Jong Hee
JH
Kim
Mi-Na
MN
Sung
Heungsup
H
Lee
Kyungwon
K
Joo
Min Young
MY
Shin
Myung Geun
MG
Suh
Soon Pal
SP
Ryang
Dong Wook
DW
D018441:Biofilms / Q000254:growth & development*; D001769:Blood / Q000382:microbiology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D056910:Republic of Korea
D003470:Culture Media