Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay.
The detection of vancomycin-resistant enterococci using a novel commercial multiplex real-time polymerase chain reaction assay (Xpert vanA/vanB, Cepheid, Sunnyvale, CA) was evaluated on 804 rectal swab specimens. Compared to enriched culture, sensitivity and negative predictive value of this method were 100%. Many false-positive results were recovered (sensitivity, 85.4%; positive predictive value, 8.7%), especially for the vanB gene.
Bourdon
Nancy
N
Centre National de la Résistance aux Antibiotiques, Laboratoire associé aux Entérocoques, Service de Microbiologie, C.H.U. Côte de Nacre, Caen, France.
Bérenger
Raphaël
R
Lepoultier
Romain
R
Mouet
Audrey
A
Lesteven
Claire
C
Borgey
France
F
Fines-Guyon
Marguerite
M
Leclercq
Roland
R
Cattoir
Vincent
V
D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D005189:False Positive Reactions; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D020713:Vancomycin Resistance
D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases