In vivo antitumor and antimetastatic activities of camptothecin encapsulated with N-trimethyl chitosan in a preclinical mouse model of liver cancer.
Application of camptothecin (CPT) is hampered due to its extreme water insolubility and unpredictable side effects. Therefore, it is essential to establish an efficient and safe protocol for the administration of camptothecin against tumor growth and metastasis. Here, we encapsulated camptothecin with N-trimethyl chitosan (CPT-TMC) and tested it on BALB/c mice subcutaneously injected with murine hepatocellular carcinoma cells at the hindlimb feet pad. CPT-TMC effectively inhibited tumor growth and lymphatic metastasis, prolonged survival time, yet without apparent toxic effects. Thus, CPT-TMC may provide a novel and effective therapeutic strategy against human advanced hepatic cancer without conspicuous systemic toxic effects.
Zhou
Lina
L
Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, People's Republic of China.
Li
Xingyi
X
Chen
Xiancheng
X
Li
Zhiyong
Z
Liu
Xianping
X
Zhou
Shengtao
S
Zhong
Qian
Q
Yi
Tao
T
Wei
Yuquan
Y
Zhao
Xia
X
Qian
Zhiyong
Z
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D002166:Camptothecin / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000188:drug therapy* / Q000378:metabolism / Q000556:secondary; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding; D005260:Female; D008113:Liver Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D042583:Lymphangiogenesis / Q000187:drug effects; D008207:Lymphatic Metastasis; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D047368:Tumor Burden / Q000187:drug effects; D042643:Vascular Endothelial Growth Factor D / Q000235:genetics / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; C118071:N-trimethyl chitosan chloride; D012333:RNA, Messenger; D042643:Vascular Endothelial Growth Factor D; D048271:Chitosan; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; D002166:Camptothecin