title: Newcastle disease virus in penguins from King George Island on the Antarctic region.
abstract: Here we report the isolation of Newcastle disease virus (NDV) from cloacal swabs obtained from penguins in the South Atlantic Antarctic region (62°08S, 58°25W). Samples of 100 penguins from King George Island were tested by real-time PCR, of which 2 (2%) were positive for NDV. The positive samples were isolated in embryonated chicken eggs and their matrix and fusion proteins genes were partially sequenced. This was complemented by the serological study performed on the blood of the same specimens, which resulted in a 33.3% rate of positivity.
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Luciano M
authors.initials: LM
authors.lastname: Thomazelli
authors.forename: Jansen
authors.initials: J
authors.lastname: Araujo
authors.forename: Danielle B
authors.initials: DB
authors.lastname: Oliveira
authors.forename: Luiz
authors.initials: L
authors.lastname: Sanfilippo
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Ferreira
authors.forename: Liana
authors.initials: L
authors.lastname: Brentano
authors.forename: Vivian H
authors.initials: VH
authors.lastname: Pelizari
authors.forename: Cristiane
authors.initials: C
authors.lastname: Nakayama
authors.forename: Rubens
authors.initials: R
authors.lastname: Duarte
authors.forename: Renata
authors.initials: R
authors.lastname: Hurtado
authors.forename: Joaquim O
authors.initials: JO
authors.lastname: Branco
authors.forename: David
authors.initials: D
authors.lastname: Walker
authors.forename: Edison L
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology; D000864:Antarctic Regions / Q000453:epidemiology; D001483:Base Sequence; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000302:isolation & purification*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D046429:Spheniscidae / Q000821:virology*