title: Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay.
abstract: The detection of vancomycin-resistant enterococci using a novel commercial multiplex real-time polymerase chain reaction assay (Xpert vanA/vanB, Cepheid, Sunnyvale, CA) was evaluated on 804 rectal swab specimens. Compared to enriched culture, sensitivity and negative predictive value of this method were 100%. Many false-positive results were recovered (sensitivity, 85.4%; positive predictive value, 8.7%), especially for the vanB gene.
authors.affiliation: Centre National de la Résistance aux Antibiotiques, Laboratoire associé aux Entérocoques, Service de Microbiologie, C.H.U. Côte de Nacre, Caen, France.
authors.forename: Nancy
authors.initials: N
authors.lastname: Bourdon
authors.forename: Raphaël
authors.initials: R
authors.lastname: Bérenger
authors.forename: Romain
authors.initials: R
authors.lastname: Lepoultier
authors.forename: Audrey
authors.initials: A
authors.lastname: Mouet
authors.forename: Claire
authors.initials: C
authors.lastname: Lesteven
authors.forename: France
authors.initials: F
authors.lastname: Borgey
authors.forename: Marguerite
authors.initials: M
authors.lastname: Fines-Guyon
authors.forename: Roland
authors.initials: R
authors.lastname: Leclercq
authors.forename: Vincent
authors.initials: V
authors.lastname: Cattoir
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D005189:False Positive Reactions; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D020713:Vancomycin Resistance
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases