Hybrid PET/CT for noninvasive pharmacokinetic evaluation of dynamic PolyConjugates, a synthetic siRNA delivery system.
Positron emission tomography/computed tomography (PET/CT) hybrid imaging can be used to gain insights into a synthetic siRNA delivery system targeted to the liver. Either siRNA or the delivery vehicle was labeled with (64)Cu via 1, 4, 7, 10- tetraazacyclododecane- 1, 4, 7, 10- tetraacetic acid (DOTA) chelation. This study confirmed that the siRNA delivery system was successfully targeted to the liver. Incorporation of the siRNA into the delivery system protected the siRNA from renal filtration long enough so that the siRNA could be delivered to the liver. PET/CT imaging was important for confirming biodistribution and for determining differences in the distribution of labeled siRNA, siRNA incorporated into the delivery system, and the delivery system without siRNA.
Mudd
Sarah R
SR
Department of Pharmaceutical Sciences, University of Wisconsin - Madison, Madison, Wisconsin 53705, USA.
Trubetskoy
Vladimir S
VS
Blokhin
Andrei V
AV
Weichert
Jamey P
JP
Wolff
Jon A
JA
D000818:Animals; D002614:Chelating Agents / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D003287:Contrast Media / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D006573:Heterocyclic Compounds, 1-Ring / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism*; D051379:Mice; D049268:Positron-Emission Tomography / Q000379:methods*; D034741:RNA, Small Interfering / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed
D002614:Chelating Agents; D003287:Contrast Media; D006573:Heterocyclic Compounds, 1-Ring; D034741:RNA, Small Interfering; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid