Accurate non-invasive image-based cytotoxicity assays for cultured cells.
BACKGROUND
The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.


RESULTS
Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.


CONCLUSIONS
This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.
Marqués-Gallego
Patricia
P
Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
den Dulk
Hans
H
Backendorf
Claude
C
Brouwer
Jaap
J
Reedijk
Jan
J
Burke
Julian F
JF
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D051379:Mice; D008853:Microscopy; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D002945:Cisplatin