title: Silk fibroin/sodium carboxymethylcellulose blended films for biotechnological applications.
abstract: The potential of silk protein is increased because of its importance as natural biopolymer for biotechnological and biomedical applications. The main disadvantage of silk fibroin films is their high brittleness. Thus, we studied blends of fibroin with other polymers to improve the film properties. Considering the possible applications of films in biomedical applications, we used a natural and biodegradable polymer as the second component. This study reports the fabrication and characterization of mulberry silk protein fibroin and sodium carboxymethylcellulose (NaCMC) blended films as potential substrates for in vitro cell culture. The blended films are investigated of their chemical interactions, morphologies, thermal, mechanical properties in addition to its swelling properties and biocompatibility. The addition of NaCMC improves the elasticity of fibroin films and its thermal properties. The change of morphology, swelling behavior and increase of surface roughness of the films were also observed in the blended films. The films become insoluble on alcohol treatment and are stable for longer duration in hydrolytic medium. The blended films are cytocompatible and supported adhesion and growth of mouse fibroblast cells. The results suggest that NaCMC blended silk fibroin films are found to be potential substratum for supporting cell adhesion and proliferation.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur-721302, India.
authors.forename: Joydip
authors.initials: J
authors.lastname: Kundu
authors.forename: Riti
authors.initials: R
authors.lastname: Mohapatra
authors.forename: S C
authors.initials: SC
authors.lastname: Kundu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D012831:Bombyx / Q000737:chemistry; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002448:Cell Adhesion; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D004548:Elasticity; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D005349:Fibroins; D002266:Carboxymethylcellulose Sodium