An easier method to identify the individual genomic composition of allopolyploid complexes.
A new method for the fast identification of the genomic composition of the cyprinid Squalius alburnoides is presented. The method is based on a length polymorphism detected in the beta-actin gene, which serves as the basis for the development of a semi-quantitative PCR.
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Universidade de Lisboa, Faculdade de Ciências de Lisboa, Centro de Biologia Ambiental-Departamento de Biologia Animal, Bloco C2-3 degrees Piso, 1749-16, Campo Grande Lisboa, Portugal. mainacio@fc.ul.pt
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D000199:Actins / Q000235:genetics; D000818:Animals; D003530:Cyprinidae / Q000235:genetics*; D016678:Genome; D023281:Genomics / Q000379:methods; D011003:Ploidies; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*
D000199:Actins