Analyzing lipid metabolism: activation and beta-oxidation of fatty acids.
There is massive gene replication predicted for the activation of fatty acids and their entry into the beta-oxidation cycle for fatty acid oxidation. These two steps in fatty acid metabolism are catalyzed by FadD and FadE enzymes with 36 genes predicted for each of these respective activities in Mycobacterium tuberculosis. Here we present methods for the cell-free assay of types of enzymes in live bacteria, as well as for fatty acid oxidation overall.
Wheeler
Paul Robert
PR
Veterinary Laboratories Agency Weybridge, Tuberculosis Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK. p.wheeler@vla.defra.gsi.gov.uk
D003066:Coenzyme A Ligases / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D008967:Molecular Biology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000378:metabolism; D010084:Oxidation-Reduction
D005227:Fatty Acids; D003066:Coenzyme A Ligases