title: Synthesis and in vitro biocompatibility assessment of a poly(amic acid) derived from ethylenediaminetetraacetic dianhydride.
abstract: Poly(amic acid) (PAA) derived from ethylenediaminetetracetic dianhydride shows great potential as a biomaterial suitable for biomedical applications. To evaluate this polymer class further, in vitro cell toxicity (WST-1/ECS, ELISA based) and cell compatibility (cell adhesion and cell proliferation) tests were conducted to establish structure-toxicity relationships. PAAs with a number-average molecular weight ranging between 100 to 200 kg/mol were synthesized at 37°C after 24 h. Porcine radial artery cells (RACs) and descending aorta endothelial cells (ECs) were seeded independently in a 96-well cell culture plate at a cell density of 5000 cells/cm(2) to observe toxic effects. Similarly, RACs and ECs were seeded independently onto PAA coated and uncoated cover slips at a cell density of 7000 cells/cm(2) to observe growth patterns. Our results showed no toxicity after 96 h of incubation and in addition, both RACs and ECs adhered and proliferated on the PAA films, preserving their phenotype during this time. The tested synthetic material seems promising as a future biomaterial and should elicit a desired cellular response upon implantation.
authors.affiliation: Biomedical Engineering Graduate Program, The University of Western Ontario, London, Ontario, Canada N6A 5B9.
authors.forename: Donna T
authors.initials: DT
authors.lastname: Padavan
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Hamilton
authors.forename: Derek R
authors.initials: DR
authors.lastname: Boughner
authors.forename: Wankei
authors.initials: W
authors.lastname: Wan
mesh_terms: D000812:Anhydrides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001555:Benzene Derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D004492:Edetic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning / Q000379:methods; D015394:Molecular Structure; D008970:Molecular Weight; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine
chemical_list: D000812:Anhydrides; D001555:Benzene Derivatives; D001672:Biocompatible Materials; D002614:Chelating Agents; D011108:Polymers; C513053:polyamic acid; D004492:Edetic Acid