The mycobacteriophage Ms6 encodes a chaperone-like protein involved in the endolysin delivery to the peptidoglycan.
Like most double-stranded (ds) DNA phages, mycobacteriophage Ms6 uses the holin-endolysin system to achieve lysis of its host. In addition to endolysin (lysA) and holin (hol) genes, Ms6 encodes three accessory lysis proteins. In this study we investigated the lysis function of Gp1, which is encoded by the gp1 gene that lies immediately upstream of lysA. Escherichia coli lysis was observed after coexpression of LysA and Gp1 in the absence of Ms6 holin. Gp1 does not belong to the holin class of proteins, and we provide evidence that it shares several characteristics with molecular chaperones. We show that Gp1 interacts with LysA, and that this interaction is necessary for LysA delivery to its target. In addition, PhoA fusions showed that, in Mycobacterium smegmatis, LysA is exported to the extracytoplasmic environment in the presence of Gp1. We also show that Gp1 is necessary for efficient M. smegmatis lysis, as Ms6 gp1 deletion results in host lysis defects. We propose that delivery of Ms6 endolysin to the murein layer is assisted by Gp1, a chaperone-like protein, in a holin-independent manner.
Catalão
Maria João
MJ
Centro de Patogénese Molecular, Unidade dos Retrovirus e Infecções Associadas, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal.
Gil
Filipa
F
Moniz-Pereira
José
J
Pimentel
Madalena
M
D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism / Q000821:virology; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009160:Mycobacteriophages / Q000235:genetics / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000378:metabolism / Q000821:virology; D010457:Peptidoglycan / Q000378:metabolism*; D021381:Protein Transport; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D018832:Molecular Chaperones; D010457:Peptidoglycan; D014764:Viral Proteins; D010450:Endopeptidases; C001143:endolysin