In-vitro culture with a tilting device in chemically defined media during meiotic maturation and early development improves the quality of blastocysts derived from in-vitro matured and fertilized porcine oocytes.
Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.
KOIKE
Takayuki
T
Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Japan.
MATSUURA
Koji
K
NARUSE
Keiji
K
FUNAHASHI
Hiroaki
H
D000818:Animals; D001757:Blastomeres / Q000166:cytology / Q000502:physiology*; D002970:Cleavage Stage, Ovum / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000295:instrumentation / Q000379:methods / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D008297:Male; D008540:Meiosis / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000166:cytology; D011247:Pregnancy; D013094:Spermatozoa / Q000166:cytology; D013552:Swine / Q000196:embryology*
D003470:Culture Media