Molecular diagnostics for personal medicine using a nanopore.
Semiconductor nanotechnology has created the ultimate analytical tool: a nanopore with single molecule sensitivity. This tool offers the intriguing possibility of high-throughput, low cost sequencing of DNA with the absolute minimum of material and preprocessing. The exquisite single molecule sensitivity obviates the need for costly and error-prone procedures like polymerase chain reaction amplification. Instead, nanopore sequencing relies on the electric signal that develops when a DNA molecule translocates through a pore in a membrane. If each base pair has a characteristic electrical signature, then ostensibly a pore could be used to analyze the sequence by reporting all of the signatures in a single read without resorting to multiple DNA copies. The potential for a long read length combined with high translocation velocity should make resequencing inexpensive and allow for haplotyping and methylation profiling in a chromosome.
Mirsaidov
Utkur M
UM
Stinson-Remick Hall, University of Notre Dame, Notre Dame, IN 46556, USA.
Wang
Deqiang
D
Timp
Winston
W
Timp
Gregory
G
D015894:Genome, Human; D006801:Humans; D057089:Pathology, Molecular / Q000379:methods*; D057285:Precision Medicine / Q000379:methods*; D045663:Quantum Dots; D017422:Sequence Analysis, DNA / Q000379:methods*