Evolutionary adaptation of recombinant shochu yeast for improved xylose utilization.
We examined the evolutionary adaptation of recombinant shochu yeast by serial anaerobic cultivation in xylose-based minimal medium. Compared with the parental strain, the adapted strain MA-S4-M1 (M1) markedly improved the growth on xylose and the anaerobic xylose consumption rate. M1 gained improved xylose utilization properties by optimizing the metabolic pathway enzymes and enhancing the uptake of xylose.
Matsushika
Akinori
A
Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. a-matsushika@aist.go.jp
Oguri
Emiko
E
Sawayama
Shigeki
S
D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology*; D000693:Anaerobiosis; D005075:Biological Evolution; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D014994:Xylose / Q000378:metabolism*
D014994:Xylose