title: Endogenous stress contributes to cardiac injury after heart transplantation in rats.
abstract: OBJECTIVE
  Stress effect plays an important role in the development of some myocardial diseases. We hypothesized it was important nosogenesis to myocardial damage and cardiac allograft vasculopathy.
  
  
  METHODS
  The transplanted hearts from Lewis to Wister rats served as allografts and from Lewis to Lewis rats as isografts based Ono's model. The differential proteins in the transplanted hearts were separated by comparative proteome, and then identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and searched by Matrix Science software system.
  
  
  RESULTS
  All transplanted hearts were characterized by lumen loss [(total vessel area-luminal area)/total vessel area] in the coronary artery 2 weeks after the operation [(2.07%+/-0.93%) vs. (27.58%+/-11.14%), P<0.01], but more predominant after 8 weeks [(2.34%+/-1.06%) vs. (72.29%+/-20.57%), P<0.01]. All samples of the left ventricle were analyzed by proteomic techniques and 37 distinct proteins involving their respective isoforms and subunits were identified. Nine proteins were correlated to endoplasmic reticulum stress effect and myocardial damage, and 2 proteins were verified by Western blot.
  
  
  CONCLUSIONS
  Stress plays an important role in cardiac allograft damage and the development of rat cardiac allograft vasculopathy.
authors.affiliation: Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China. zhuangweihy@sina.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Zhuang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Haihe
authors.initials: H
authors.lastname: Jiang
authors.forename: Weixing
authors.initials: W
authors.lastname: Zhang
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
mesh_terms: D034841:Abdominal Cavity; D000818:Animals; D003327:Coronary Disease / Q000209:etiology*; D016027:Heart Transplantation / Q000009:adverse effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic
chemical_list: D020543:Proteome