Up-regulated microRNA-146a negatively modulate Helicobacter pylori-induced inflammatory response in human gastric epithelial cells.
Helicobacter pylori (H. pylori) is a major human pathogenic bacterium in gastric mucosa. However, the regulatory mechanism of H. pylori-induced immune response is not clear. MicroRNAs (miRNAs) have recently emerged as key post-transcriptional regulators of gene expression, and their role in H. pylori infection is just beginning to be explored. Here, we first reported that H. pylori infection up-regulated the expression of miR-146a in gastric epithelial cells as well as in gastric mucosal tissues in NF-κB-dependent manner. In turn, miR-146a may downregulate the expression of target genes, interleukin-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6). Furthermore, miR-146a negatively regulated H. pylori-triggered interleukin (IL)-8, growth-related oncogene (GRO)-α, and macrophage inflammatory protein (MIP) -3α through diminishing NF-κB activity. In conclusion, H. pylori-induced miR-146a plays a potential role in a negative feedback loop to modulate the inflammation by targeting IRAK1 and TRAF6.
Liu
Zhen
Z
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China.
Xiao
Bin
B
Tang
Bin
B
Li
Bosheng
B
Li
Na
N
Zhu
Endong
E
Guo
Gang
G
Gu
Jiang
J
Zhuang
Yuan
Y
Liu
Xiaofei
X
Ding
Honglei
H
Zhao
Xiaoyan
X
Guo
Hong
H
Mao
Xuhu
X
Zou
Quanming
Q
D000328:Adult; D000368:Aged; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005786:Gene Expression Regulation; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007249:Inflammation; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis; D048029:TNF Receptor-Associated Factor 6 / Q000096:biosynthesis*
C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D048029:TNF Receptor-Associated Factor 6; D053592:Interleukin-1 Receptor-Associated Kinases