Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery.
We have studied the effect of enhanced oxygen delivery by perfluorocarbons on the differentiation of C2C12 cells. The extent of differentiation was assessed by means of phase contrast/fluorescence microscopy, active tension measurement and the glucose consumption/lactate production rates. We found that enhanced oxygen delivery is suitable for full differentiation of C2C12 cells.
Fujita
Hideaki
H
Toyota Central Research and Development Labs., Inc., 41-1 Yokomichi, Nagakute, Aichi 480-1192, Japan.
Shimizu
Kazunori
K
Morioka
Yuki
Y
Nagamori
Eiji
E
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005466:Fluorocarbons / Q000493:pharmacokinetics*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*
D005466:Fluorocarbons; D010100:Oxygen