title: Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.
abstract: Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation. In this condition, nattokinase activity was 630 unit/ml at 12 h. In batch culture of B. subtilis in fermentor, the highest nattokinase activity of 3400 unit/ml was obtained at 10h with 50 g/L of peptone supplementation. From the batch kinetics data, it was shown that nattokinase production was growth-associated and culture should be harvested before stationary phase for maximum nattokinase production. In fed-batch culture of B. subtilis using pH-stat feeding strategy, cell growth (optical density monitored at 600 nm) increased to ca. 100 at 22 h, which was 2.5 times higher than that in batch culture. The highest nattokinase activity was 7100 unit/ml at 19 h, which was also 2.1 times higher than that in batch culture.
authors.affiliation: Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Young-Han
authors.initials: YH
authors.lastname: Cho
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Song
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: In Young
authors.initials: IY
authors.lastname: Lee
authors.forename: Sang Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Hyeon Shup
authors.initials: HS
authors.lastname: Kim
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Beom Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D013381:Subtilisins / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D003470:Culture Media; D013381:Subtilisins; C053771:nattokinase