Genetic modification of Teredinibacter turnerae, an endosymbiont with biotechnological potential.
Teredinibacter turnerae belongs to a group of biotechnologically relevant bacteria. Gene transfer into T. turnerae was achieved by using pPROBE'-gfp[ASV] derived plasmids through conjugative mating with Escherichia coli DH5alpha pRK2073. Transferred plasmids were stably maintained and T. turnerae could also act as a donor to transfer these mobilizable plasmids. Constructs for both constitutive and IPTG-inducible gene expression were obtained, representing new tools for gene overexpression in T. turnerae.
Senra
Marcus V X
MV
Laboratório de Genética Molecular de Eucariontes e Simbiontes, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Vizzoni
Vinicius F
VF
Trindade-Silva
Amaro E
AE
Giannini
Ana L M
AL
Soares
Carlos A G
CA
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D049872:Bivalvia / Q000382:microbiology*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D020563:Gammaproteobacteria / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009586:Nitrogen Fixation; D010957:Plasmids; D013559:Symbiosis
D049452:Green Fluorescent Proteins; D007544:Isopropyl Thiogalactoside