Characterization of murine non-adherent bone marrow cells leading to recovery of endogenous hematopoiesis.
Non-adherent bone marrow-derived cells (NA-BMCs) are a mixed cell population that can give rise to multiple mesenchymal phenotypes and that facilitates hematopoietic recovery. We characterized NA-BMCs by flow cytometry, fibroblast colony-forming units (CFU-f), real-time PCR, and in in vivo experiments. In comparison to adherent cells, NA-BMCs expressed high levels of CD11b(+) and CD90(+) within the CD45(+) cell fraction. CFU-f were significantly declining over the cultivation period, but NA-BMCs were still able to form CFU-f after 5 days. Gene expression analysis of allogeneic NA-BMCs compared to bone marrow (BM) indicates that NA-BMCs contain stromal, mesenchymal, endothelial cells and monocytes, but less osteoid, lymphoid, and erythroid cells, and hematopoietic stem cells. Histopathological data and analysis of weight showed an excellent recovery and organ repair of lethally irradiated mice after NA-BMC transplantation with a normal composition of the BM.
Fricke
Stephan
S
Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstraße 01, 04103 Leipzig, Germany. stephan.fricke@izi.fraunhofer.de
Fricke
Christian
C
Oelkrug
Christopher
C
Hilger
Nadja
N
Schönfelder
Uta
U
Kamprad
Manja
M
Lehmann
Jörg
J
Boltze
Johannes
J
Emmrich
Frank
F
Sack
Ulrich
U
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D015704:CD4 Antigens / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
D015415:Biomarkers; D015704:CD4 Antigens