Inhibition of twisting of a green fluorescent protein-like chromophore by metal complexation.
Substitution of a pyridyl for the hydroxyphenyl moiety in the Green Fluorescent Protein analog p-hydroxybenzylidene-dimethylimidiazolinone produces a chromophore which "turns on" fluorescence in the presence of Zn(2+) or Cd(2+) ions. Such a phenomenon provides "proof of principle" for using GFP chromophores in a variety of sensing applications.
Baldridge
Anthony
A
School of Chemistry and Biochemistry, 901 Atlantic Dr, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Solntsev
Kyril M
KM
Song
Charles
C
Tanioka
Tatsuro
T
Kowalik
Janusz
J
Hardcastle
Kenneth
K
Tolbert
Laren M
LM
D001593:Benzyl Compounds / Q000737:chemistry*; D002104:Cadmium / Q000737:chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D048288:Imidazolines / Q000737:chemistry*; D008024:Ligands; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry
D001593:Benzyl Compounds; D056831:Coordination Complexes; D048288:Imidazolines; D008024:Ligands; C533767:p-hydroxybenzylidenedimethylimidazolinone; D002104:Cadmium; D049452:Green Fluorescent Proteins; D015032:Zinc