title: Microfluidic dissociation and clearance of Alzheimer's beta-amyloid aggregates.
abstract: The abnormal aggregation of beta-amyloid peptide (Abeta) in the brain is a major histopathological feature of Alzheimer's disease. Herein, we first report on microfluidic dissociation and clearance analysis of pre-formed Abeta aggregates for parallel screening of aggregate destabilizers. As a proof of the concept for the microfluidic platform, we investigated (1) microfluidics-based clearance of metal ion-induced Abeta aggregates using different types of metal chelators, (2) the clearance effect of deferoxamine on Abeta aggregates within microchannels, (3) comparison between destabilized Abeta dissociated from pre-formed Abeta aggregates and remaining deposits within the microchannels both before and after the clearance, and (4) secondary structure change in Abeta deposits by the clearance treatment. The microfluidics-based clearance system should be suitable for efficient screening of chemical candidates to enhance the clearance of Abeta deposits prior to their in vivo evaluation.
authors.affiliation: Institute for the BioCentury and Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 335 Science Road, Daejeon 305 701, Republic of Korea.
authors.forename: Joon Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Chan Beum
authors.initials: CB
authors.lastname: Park
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D003676:Deferoxamine / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D044085:Microfluidics / Q000379:methods*; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D016229:Amyloid beta-Peptides; D007502:Iron Chelating Agents; D012996:Solutions; D007501:Iron; D003676:Deferoxamine