title: [Abnormal expression of programmed cell death 5 gene in multiple myeloma patients].
abstract: The aim of this study was to investigate the gene expression of programmed cell death 5 (pdcd5) in plasma and bone marrow cells from patients with multiple myeloma (MM). Enzyme liked immunosorbent assay (ELISA) and real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) were used to examine pdcd5 gene expression in plasma and marrow cells in 45 MM patients and 20 normal controls. The results showed that serum levels of PDCD5 protein in 45 MM patients were lower significantly compared with the normal controls and 20 responsive patients after chemotherapy, their plasma levels were (16.91 +/- 0.28) ng/ml, (19.11 +/- 0.29) ng/ml and (17.94 +/- 0.154) ng/ml respectively (p < 0.05). The pdcd5 gene expression levels detected by RQ-RT-PCR in 45 MM patients were lower significantly compared with the normal controls, their pdcd5 gene expression levels were 0.64 +/- 0.47 and 1.28 +/- 1.21 respectively (p < 0.05). It is concluded that the PDCD5 protein expression levels are low in patients with MM. These findings suggest that abnormal expression of pdcd5 may be involved in the pathogenesis of MM.
authors.affiliation: Peking University People Hospital, Peking University Institute of Hematology, Beijing 100044, China.
authors.forename: Li
authors.initials: L
authors.lastname: Bao
authors.forename: Guo-Rui
authors.initials: GR
authors.lastname: Ruan
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: Lu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Lu
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Niu
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Xie
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Qin
authors.forename: Ling-Di
authors.initials: LD
authors.lastname: Li
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Li
authors.forename: Yan-Rong
authors.initials: YR
authors.lastname: Liu
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Chen
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001854:Bone Marrow Cells / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology*; D009363:Neoplasm Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human