The 4-acetylantroquinonol B isolated from mycelium of Antrodia cinnamomea inhibits proliferation of hepatoma cells.
BACKGROUND
Antrodia cinnamomea is known for its antihepatoma activity, yet the identity of its active compound was unclear. In this study, a 5-ton fermenter was used to prepare sufficient mycelium of A. cinnamomea for active compound isolation and identification.


RESULTS
Using antiproliferative activity toward HepG2 cells as guidance in the isolation process, 4-acetylantroquinonol B was purified and identified to be the major bioactive compound of A. cinnamomea cultivated by submerged fermentation. The median effective doses (EC(50)) of 4-acetylantroquinonol B for HepG2 cells were 0.10 +/- 0.00 and 0.08 +/- 0.00 microg mL(-1) for 72 and 96 h treatments, respectively. The selective indices of 4-acetylantroquinonol B were 100 and 125 for 72 and 96 h treatments, respectively, indicating that this compound had high selective activity for hepatoma cells.


CONCLUSIONS
4-Acetylantroquinonol B is the major antihepatoma constituent of Antrodia cinnamomea mycelium produced by submerged fermentation.
Lin
Yu-Wei
YW
Institute of Food Science and Technology, National Taiwan University, No. 1 Sec. 4 Roosevelt Road, Taipei, Taiwan, ROC.
Pan
Jih-Hung
JH
Liu
Rui Hai
RH
Kuo
Yueh-Hsiung
YH
Sheen
Lee-Yen
LY
Chiang
Been-Huang
BH
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D055491:Antrodia / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D049109:Cell Proliferation / Q000187:drug effects*; D003512:Cyclohexanones / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005285:Fermentation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D025282:Mycelium
D000970:Antineoplastic Agents; D001688:Biological Products; D003512:Cyclohexanones; C555021:4-acetylantroquinonol B; D015107:4-Butyrolactone