[Immunohistochemical expression of P53 and Ki-67 in diethylnitrosamine-induced carcinomas esophageal: experimental model].
OBJECTIVE
To evaluate the expression of P53 and Ki-67 during esophageal diethylnitrosamine (DEN)-induced carcinogenesis in 100 mice by immunohistochemistry.


METHODS
The animals were assigned to 4 groups, receiving water and food ad libitum. Control groups I and II received weekly esophageal gavage with cold (room temperature) or hot (60-70 degrees C) water, respectively. Experimental groups III and IV were treated with DEN for 3 consecutive days during the week, and one weekly gavage as above. The mice were sacrificed in different periods from day 30 to day 150 after the beginning of the experiment, for collection of esophageal samples which were then submitted to microscopic and immunohistochemical analyses. The temperature of the water administered by gavage was not related to the frequency of esophageal tumors.


RESULTS
The expression of Ki-67 was significantly higher in high-grade intraepithelial lesion (I.L.), and the expression of P53 was also higher in low-grade I.L.


CONCLUSIONS
The results emphasize the direct relationship of the carcinogenic process with early cell alterations detected by immunohistochemistry.
de Castro Junior
Miguel Angelo Martins
MA
FURG, BR.
Kruel
Cleber Dario Pinto
CD
Meurer
Luise
L
de Castro
Angela Potter
AP
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000276:immunology*; D004052:Diethylnitrosamine / Q000008:administration & dosage; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000276:immunology*; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D051379:Mice; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D004052:Diethylnitrosamine