Antimycobacterial activity in vitro of pigments isolated from Antarctic bacteria.
In this study, we describe the antimycobacterial activity of two pigments, violacein, a purple violet pigment from Janthinobacterium sp. Ant5-2 (J-PVP), and flexirubin, a yellow-orange pigment from Flavobacterium sp. Ant342 (F-YOP). These pigments were isolated from bacterial strains found in the land-locked freshwater lakes of Schirmacher Oasis, East Antarctica. The minimum inhibitory concentrations (MICs) of these pigments for avirulent and virulent mycobacteria were determined by the microplate Alamar Blue Assay (MABA) and Nitrate Reductase Assay (NRA). Results indicated that the MICs of J-PVP and F-YOP were 8.6 and 3.6 μg/ml for avirulent Mycobacterium smegmatis mc²155; 5 and 2.6 μg/ml for avirulent Mycobacterium tuberculosis mc²6230; and 34.4 and 10.8 μg/ml for virulent M. tuberculosis H₃₇Rv, respectively. J-PVP exhibited a ~15 times lower MIC for Mycobacterium sp. than previously reported for violacein pigment from Chromobacterium violaceum, while the antimycobacterial effect of F-YOP remains undocumented. Our results indicate these pigments isolated from Antarctic bacteria might be valuable lead compounds for new antimycobacterial drugs used for chemotherapy of tuberculosis.
Mojib
Nazia
N
Department of Biology, University of Alabama at Birmingham, 35294-1170, USA.
Philpott
Rachel
R
Huang
Jonathan P
JP
Niederweis
Michael
M
Bej
Asim K
AK
D000864:Antarctic Regions; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology; D020562:Betaproteobacteria / Q000737:chemistry*; D055808:Drug Discovery; D024881:Drug Resistance, Bacterial; D005417:Flavobacterium / Q000737:chemistry*; D007211:Indoles / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*; D020102:Mycobacterium smegmatis / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010860:Pigments, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D011090:Polyenes / Q000302:isolation & purification / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D007211:Indoles; D010860:Pigments, Biological; D011090:Polyenes; C017313:flexirubins; C063155:violacein