Covering all bases in HIV research: unveiling a hidden world of viral evolution.
The advent of next-generation sequencing technologies has greatly impacted genomic research by providing a means for increasing the amount of sequence information in a cost effective fashion. Among the major next-generation sequencing technologies, the Roche 454 platform has been widely adopted in HIV research. We discuss a broad range of applications of the 454 pyrosequencing platform in the HIV field, with a particular emphasis on antiretroviral therapy and virus-host interaction-related research. We also highlight some of the bioinformatics challenges, as well as advantages and potential limitations of this "deep" sequencing tool, and hint at future research applications.
Vrancken
Bram
B
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium. bram.vrancken@rega.kuleuven.be
Lequime
Sébastian
S
Theys
Kristof
K
Lemey
Philippe
P
D019380:Anti-HIV Agents / Q000627:therapeutic use; D019143:Evolution, Molecular; D006678:HIV / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
D019380:Anti-HIV Agents; D012367:RNA, Viral