title: Hippocampus and dentate gyrus of the Cebus monkey: architectonic and stereological study.
abstract: Behavioral, electrophysiological, and anatomical assays of non-human primates have provided substantial evidence that the hippocampus and dentate gyrus are essential for memory consolidation. However, a single anatomical and stereological investigation of these regions has been done in New World primates to complement those assays. The aim of the present study was to describe the cyto-, myelo-, and histochemical architecture of the hippocampus and dentate gyrus, and to use the optical fractionator method to estimate the number of neurons in the hippocampal pyramidal and granular neurons in the dentate gyrus of the Cebus monkey. NeuN immunolabeling, lectin histochemical staining with Wisteria floribunda agglutinin (WFA), enzyme-histochemical detection of NADPH-diaphorase activity and Gallyas silver staining were used to define the layers and limits of the hippocampal fields and dentate gyrus. A comparative analysis of capuchin (Cebus apella) and Rhesus (Macaca mulatta) monkeys revealed similar structural organization of these regions but significant differences in the regional distribution of neurons. C. apella were found to have 1.3 times fewer pyramidal and 3.5 times fewer granular neurons than M. mulatta. Taken together the architectonic and stereological data of the present study suggest that hippocampal and dentate gyrus neural networks in the C. apella and M. mulatta may contribute to hippocampal-dentate gyrus-dependent tasks in different proportions.
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Lab of Investigations in Neurodegeneration and Infection, Belém, Pará, Brazil.
authors.forename: Cristovam
authors.initials: C
authors.lastname: Guerreiro-Diniz
authors.forename: Roberta Bentes
authors.initials: RB
authors.lastname: de Melo Paz
authors.forename: Mayra Hermínia Simões
authors.initials: MH
authors.lastname: Hamad
authors.forename: Carlos Santos
authors.initials: CS
authors.lastname: Filho
authors.forename: Adriano Augusto Vilhena
authors.initials: AA
authors.lastname: Martins
authors.forename: Heitor Bastos
authors.initials: HB
authors.lastname: Neves
authors.forename: Elane Domenica
authors.initials: ED
authors.lastname: de Souza Cunha
authors.forename: Gisele Cristina
authors.initials: GC
authors.lastname: Alves
authors.forename: Lia Amaral
authors.initials: LA
authors.lastname: de Sousa
authors.forename: Ivanira Amaral
authors.initials: IA
authors.lastname: Dias
authors.forename: Nonata
authors.initials: N
authors.lastname: Trévia
authors.forename: Aline Andrade
authors.initials: AA
authors.lastname: de Sousa
authors.forename: Aline
authors.initials: A
authors.lastname: Passos
authors.forename: Nara
authors.initials: N
authors.lastname: Lins
authors.forename: João Bento
authors.initials: JB
authors.lastname: Torres Neto
authors.forename: Pedro Fernando
authors.initials: PF
authors.lastname: da Costa Vasconcelos
authors.forename: Cristovam Wanderley
authors.initials: CW
authors.lastname: Picanço-Diniz
mesh_terms: D000818:Animals; D002428:Cebus / Q000033:anatomy & histology*; D006624:Hippocampus / Q000033:anatomy & histology*; D008253:Macaca mulatta / Q000033:anatomy & histology; D009474:Neurons / Q000166:cytology*; D013194:Staining and Labeling