Stromal cell-derived factor-1 promotes migration of cells from the upper rhombic lip in cerebellar development.
During cerebellar development, the chemokine stromal cell-derived factor-1 alpha (SDF-1 alpha) has been shown to play an important role in recruiting cells from the upper rhombic lip (URL) and external granule cell layer (EGL). However, its function in cerebellar development is still poorly understood. Our results have demonstrated that SDF-1 is necessary for EGL development, and URL cells stream to the SDF-1 source in vitro. Results of embryonic URL explant assays and transwell assays indicated that SDF-1 induces neural cell migration from the URL region in chemotactic and chemokinetic responses. The time-lapse results showed that the migration speed of granule cell progenitors out of the URL was accelerated by the addition of recombinant SDF-1 alpha. Collectively, our study shows that SDF-1 increases the motility of URL cells in the absence of a gradient and promotes the migration of granule cell progenitors during cerebellar development.
Yu
Tao
T
School of Life Sciences, Shanghai University, Shanghai, People's Republic of China. emmayu@staff.shu.edu.cn
Huang
Hai
H
Li
Hui-Fang
HF
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D054377:Chemokine CXCL12 / Q000172:deficiency* / Q000494:pharmacology; D018920:Coculture Techniques / Q000379:methods; D004622:Embryo, Mammalian; D005136:Eye Proteins / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism
D054377:Chemokine CXCL12; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins