title: Hydrothermal synthesis and characterization of hydroxyapatite and fluorhydroxyapatite nano-size powders.
abstract: Pure hydroxyapatite (HAp) and fluoride-containing apatite powders (FHAp) were synthesized using a hydrothermal method. The powders were assessed by x-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscope (SEM) and F-selective electrode. X-ray diffraction results revealed the formation of single phase apatite structure for all the compositions synthesized in this work. However, the addition of a fluoride ion led to a systematic shift in the (3 0 0) peak of the XRD pattern as well as modifications in the FTIR spectra. It was found that the efficiency of fluoride ion incorporation decreased with the increase in the fluoride ion content. Fluorine incorporation efficiency was around 60% for most of the FHAp samples prepared in the current study. Smaller and less agglomerated particles were obtained by fluorine substitution. The bioactivity of the powder samples with different fluoride contents was compared by performing cell proliferation, alkaline phosphatase (ALP) and Alizarin red staining assays. Human osteoblast cells were used to assess the cellular responses to the powder samples in this study. Results demonstrated a strong dependence of different cell activities on the level of fluoridation.
authors.affiliation: School of Metallurgy and Materials Engineering, Iran University of Science and Technology, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Montazeri
authors.forename: Jafar
authors.initials: J
authors.lastname: Javadpour
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
authors.forename: Sayfoddin
authors.initials: S
authors.lastname: Javadian
mesh_terms: D002478:Cells, Cultured; D003460:Crystallization / Q000379:methods; D017886:Durapatite / Q000138:chemical synthesis* / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D006882:Hydroxyapatites / Q000138:chemical synthesis* / Q000494:pharmacology; D008422:Materials Testing; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010316:Particle Size; D011208:Powders; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D006882:Hydroxyapatites; D011208:Powders; D014867:Water; C028869:fluor-hydroxylapatite; D017886:Durapatite