Hippocampus and dentate gyrus of the Cebus monkey: architectonic and stereological study.
Behavioral, electrophysiological, and anatomical assays of non-human primates have provided substantial evidence that the hippocampus and dentate gyrus are essential for memory consolidation. However, a single anatomical and stereological investigation of these regions has been done in New World primates to complement those assays. The aim of the present study was to describe the cyto-, myelo-, and histochemical architecture of the hippocampus and dentate gyrus, and to use the optical fractionator method to estimate the number of neurons in the hippocampal pyramidal and granular neurons in the dentate gyrus of the Cebus monkey. NeuN immunolabeling, lectin histochemical staining with Wisteria floribunda agglutinin (WFA), enzyme-histochemical detection of NADPH-diaphorase activity and Gallyas silver staining were used to define the layers and limits of the hippocampal fields and dentate gyrus. A comparative analysis of capuchin (Cebus apella) and Rhesus (Macaca mulatta) monkeys revealed similar structural organization of these regions but significant differences in the regional distribution of neurons. C. apella were found to have 1.3 times fewer pyramidal and 3.5 times fewer granular neurons than M. mulatta. Taken together the architectonic and stereological data of the present study suggest that hippocampal and dentate gyrus neural networks in the C. apella and M. mulatta may contribute to hippocampal-dentate gyrus-dependent tasks in different proportions.
Guerreiro-Diniz
Cristovam
C
Universidade Federal do Pará, Instituto de Ciências Biológicas, Lab of Investigations in Neurodegeneration and Infection, Belém, Pará, Brazil.
de Melo Paz
Roberta Bentes
RB
Hamad
Mayra Hermínia Simões
MH
Filho
Carlos Santos
CS
Martins
Adriano Augusto Vilhena
AA
Neves
Heitor Bastos
HB
de Souza Cunha
Elane Domenica
ED
Alves
Gisele Cristina
GC
de Sousa
Lia Amaral
LA
Dias
Ivanira Amaral
IA
Trévia
Nonata
N
de Sousa
Aline Andrade
AA
Passos
Aline
A
Lins
Nara
N
Torres Neto
João Bento
JB
da Costa Vasconcelos
Pedro Fernando
PF
Picanço-Diniz
Cristovam Wanderley
CW
D000818:Animals; D002428:Cebus / Q000033:anatomy & histology*; D006624:Hippocampus / Q000033:anatomy & histology*; D008253:Macaca mulatta / Q000033:anatomy & histology; D009474:Neurons / Q000166:cytology*; D013194:Staining and Labeling