title: Potent delivery of functional proteins into Mammalian cells in vitro and in vivo using a supercharged protein.
abstract: The inability of proteins to potently penetrate mammalian cells limits their usefulness as tools and therapeutics. When fused to superpositively charged GFP, proteins rapidly (within minutes) entered five different types of mammalian cells with potency up to approximately 100-fold greater than that of corresponding fusions with known protein transduction domains (PTDs) including Tat, oligoarginine, and penetratin. Ubiquitin-fused supercharged GFP when incubated with human cells was partially deubiquitinated, suggesting that proteins delivered with supercharged GFP can access the cytosol. Likewise, supercharged GFP delivered functional, nonendosomal recombinase enzyme with greater efficiencies than PTDs in vitro and also delivered functional recombinase enzyme to the retinae of mice when injected in vivo.
authors.forename: James J
authors.initials: JJ
authors.lastname: Cronican
authors.forename: David B
authors.initials: DB
authors.lastname: Thompson
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Beier
authors.forename: Brian R
authors.initials: BR
authors.lastname: McNaughton
authors.forename: Constance L
authors.initials: CL
authors.lastname: Cepko
authors.forename: David R
authors.initials: DR
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D016503:Drug Delivery Systems; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012160:Retina / Q000378:metabolism
chemical_list: D011506:Proteins; D049452:Green Fluorescent Proteins