title: Fixation methods for electron microscopy of human and other liver.
abstract: For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
authors.affiliation: Electron Microscope Unit, University of Maastricht, 6200 MD Maastricht, The Netherlands. eddie@wisse.be
authors.forename: Eddie
authors.initials: E
authors.lastname: Wisse
authors.forename: Filip
authors.initials: F
authors.lastname: Braet
authors.forename: Hans
authors.initials: H
authors.lastname: Duimel
authors.forename: Celien
authors.initials: C
authors.lastname: Vreuls
authors.forename: Ger
authors.initials: G
authors.lastname: Koek
authors.forename: Steven W M
authors.initials: SW
authors.lastname: Olde Damink
authors.forename: Maartje A J
authors.initials: MA
authors.lastname: van den Broek
authors.forename: Bart
authors.initials: B
authors.lastname: De Geest
authors.forename: Cees H C
authors.initials: CH
authors.lastname: Dejong
authors.forename: Chise
authors.initials: C
authors.lastname: Tateno
authors.forename: Peter
authors.initials: P
authors.lastname: Frederik
mesh_terms: D000818:Animals; D001706:Biopsy; D002478:Cells, Cultured; D016591:Histocytological Preparation Techniques / Q000379:methods; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010477:Perfusion; D016707:Tissue Fixation / Q000379:methods*