An assessment of chromosomal rearrangements in neopolyploids of Lilium hybrids.
Two types of newly induced polyploids (neopolyploids) of Lilium hybrids were monitored for the occurrence of chromosomal rearrangements through genomic in situ hybridization (GISH) technique. One of the populations was obtained through crossing an allotriploid Longiflorum x Oriental hybrid (LLO) with an allotetraploid Longiflorum x Trumpet hybrid (LLTT), both of which were derived from somatic chromosome doubling. The other type of allopolyploid population was derived from meiotic chromosome doubling in which numerically unreduced (2n) gametes from two different interspecific hybrids, namely, Longiflorum x Asiatic (LA) and Oriental x Asiatic (OA), were used to get backcross progeny with the Asiatic parents. GISH clearly discriminated the three constituent genomes (L, T, and O) in the complements of the progeny obtained from mitotic chromosome doubling. A total of 26 individuals were analyzed from this population and there was no evidence of chromosomal rearrangements. However, in the case of meiotically doubled allopolyploid progeny, considerable frequencies of chromosomal rearrangements were observed through GISH. The so-called chromosomal rearrangements in meiotic polyploids are the result of homoeologous recombination rather than translocations. Furthermore, evidence for the occurrence of meiotic recombination in the LA hybrids has been confirmed with GISH on meiotic chromosomes. Thus, there was evidence that neopolyploids of Lilium hybrids did not possess any noticeable chromosome rearrangements.
Xie
Songlin
S
College of Horticulture, Northwest A&F University, Yangling Shaanxi, People's Republic of China.
Khan
Nadeem
N
Ramanna
M S
MS
Niu
Lixin
L
Marasek-Ciolakowska
Agnieszka
A
Arens
Paul
P
van Tuyl
Jaap M
JM
D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D018745:Genome, Plant / Q000235:genetics; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D027762:Lilium / Q000235:genetics*; D011123:Polyploidy; D014178:Translocation, Genetic