Enzymes responsible for the conversion of N alpha-[(Benzyloxy)carbonyl]-D-lysine to N alpha-[(Benzyloxy)carbonyl]-D-aminoadipic acid by Rhodococcus sp. AIU Z-35-1.
The enzymes responsible for the conversion of N(alpha)-[(benzyloxy)carbonyl]-D-lysine (N(alpha)-Z-D-lysine) to N(alpha)-Z-D-aminoadipic acid (N(alpha)-Z-D-AAA) by Rhodococcus sp. AIU Z-35-1 were identified. N(alpha)-Z-D-Lysine was first converted to N(alpha)-Z-D-aminoadipic delta-semialdehyde (N(alpha)-Z-D-AASA) by D-specific amino acid deaminase, whereas N(alpha)-Z-L-lysine was converted to N(alpha)-Z-L-AASA by L-specific amino acid oxidase. The resulting N(alpha)-Z-D-AASA was then converted to N(alpha)-Z-D-AAA by the same aldehyde dehydrogenase that is responsible for N(alpha)-Z-L-AASA oxidation. The product amount of the D-specific amino acid deaminase reached the maximum at one day of cultivation in the L-lysine medium. The aldehyde dehydrogenase reached the maximum at three days of cultivation.
Isobe
Kimiyasu
K
Department of Biological Chemistry and Food Science, Iwate University, 18-8 Ueda-3, Morioka 020-8550, Japan. kiso@iwate-u.ac.jp
Fukuda
Nahoko
N
Nagasawa
Shouko
S
Saitou
Kaoru
K
D015074:2-Aminoadipic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D050830:L-Amino Acid Oxidase / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology*; D013997:Time Factors
D001426:Bacterial Proteins; C526190:N(alpha)-benzyloxycarbonyl-aminoadipic acid; D015074:2-Aminoadipic Acid; C037206:N(alpha)-benzyloxycarbonyllysine; D000444:Aldehyde Dehydrogenase; D050830:L-Amino Acid Oxidase; D008239:Lysine