title: Positive effects of ATP on G-quadruplex-hemin DNAzyme-mediated reactions.
abstract: Some G-quadruplex-hemin complexes can be used as peroxidase-mimicking DNAzymes, catalyzing H(2)O(2)-mediated reactions such as the oxidation of 2,2'-azinobis (3-ethylbenzothiozoline)-6-sulfonic acid (ABTS) by H(2)O(2). However, some challenges, for example, the relatively low catalytic activity and the disproportionation of the reaction product ABTS*(+), may seriously restrict further development and applications of these complexes. Here, we demonstrated the positive effect of adenosine triphosphate (ATP) on G-quadruplex-hemin DNAzyme-mediated catalytic reactions. The presence of ATP not only improved the catalytic activity of G-quadruplex-hemin DNAzymes, but also inhibited the disproportionation of ABTS*(+). These observations may improve the performance of existing G-quadruplex-hemin DNAzyme-based chemical sensors, for example, the Ag(+)-detection method that uses G-quadruplex-hemin DNAzymes, and widen the application range of G-quadruplex-hemin DNAzymes. We also demonstrated that the phosphate groups, nucleobase, and sugar of ATP determine the reaction-promoting ability of ATP. These observations may be helpful in the design of highly efficient enhancers for G-quadruplex-hemin DNAzymes.
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Nankai University, Tianjin 300071, P. R. China. kongdem@nankai.edu.cn
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Kong
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Han-Xi
authors.initials: HX
authors.lastname: Shen
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry*; D002384:Catalysis; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D012834:Silver / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D012834:Silver; D006427:Hemin; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide