Apoptosis induced by hematoporphyrin monomethyl ether combined with He-Ne laser irradiation in vitro on canine breast cancer cells.
Hematoporphyrin monomethyl ether (HMME) is a novel and promising porphyrin-related photosensitizer for photodynamic therapy (PDT). The aim of this study was to investigate HMME-induced apoptosis in CHMm cells, a canine breast cancer cell line. CHMm cells were treated with HMME and a He-Ne laser at a wavelength of 632.8 nm. Cell viability was determined using the trypan blue exclusion assay. Apoptosis was analyzed using Hoechst 33258, AO/EB, Annexin V/PI staining and single-cell gel electrophoresis (comet assay). Apoptotic morphology was further confirmed by Giemsa staining and transmission electron microscopy. Rates of apoptosis increased following PDT-HMME treatment in a time-dependent manner. Taken together, these results demonstrated that apoptosis plays a major role in PDT-HMME-induced reduction in the viability of CHMm cells.
Liu
Yun
Y
Department of Veterinary Clinic, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Ma
Xing Q
XQ
Jin
Peng
P
Li
Hua T
HT
Zhang
Rong R
RR
Ren
Xiao L
XL
Wang
Hong B
HB
Tang
Damu
D
Tian
Wen R
WR
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy / Q000379:methods; D004283:Dog Diseases / Q000188:drug therapy* / Q000601:surgery; D004285:Dogs; D005260:Female; D006371:Helium; D006415:Hematoporphyrins / Q000627:therapeutic use*; D054020:Lasers, Gas / Q000627:therapeutic use*; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy* / Q000601:surgery; D009356:Neon; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use*
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C494379:hematoporphyrin monomethyl ether; D006371:Helium; D009356:Neon