title: Development of D-allose sensor on the basis of three strategic enzyme reactions.
abstract: Rare sugars are defined as monosaccharides and their derivatives that rarely exist in nature, according to the International Society of Rare Sugars. D-Allose (3-epi d-glucose) is one of the rare sugars, for which various physiological activities have recently been found, with increasing attention to its applications to bio-industry. Until now, however, there is no convenient method of measuring these sugars in a specific manner. For detecting D-allose, three consecutive enzyme reactions were devised by fabricating of a reaction batch chamber packed with L-rhamnose isomerase (LRI), D-tagatose 3-epimerase (DTE) and a screen-printed electrode, on which D-fructose dehydrogenase (DFDH) was immobilized. To obtain a substantial sensing system, extensive experimental parameters were optimized. These included the concentration of photo-crosslinkable poly (vinyl alcohol) bearing stilbazolium groups (PVA-SbQ), reaction ratios, and temperature of the batch chamber. By adopting the three consecutive enzyme reactions, an undesirable reverse reaction was minimized. As a result, the developed sensor system exhibited a good linear response on D-allose in the range from 0.1 to 50 mM (r(2)=0.998). The system has an apparent advantage over the previous chromatography approach in terms of simplicity and inexpensiveness.
authors.affiliation: Division of Glyco-Bioindustry, Life Science Research Center, Institute of Research Promotion, Kagawa University, Japan.
authors.forename: Nobumitsu
authors.initials: N
authors.lastname: Miyanishi
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Nakakita
authors.forename: Wataru
authors.initials: W
authors.lastname: Sumiyoshi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Okuma
authors.forename: Ken
authors.initials: K
authors.lastname: Izumori
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D009097:Multienzyme Complexes / Q000737:chemistry*
chemical_list: D009097:Multienzyme Complexes; C002055:allose; D005947:Glucose