title: Proteomic analysis of protein expression affected by peroxiredoxin V knock-down in hypoxic kidney.
abstract: Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and the nucleus. Prdx V has been reported to protect a wide range of cellular environments as an antioxidant enzyme, and its dysfunctions may be implicated in several diseases, such as cancer, inflammation, and neurodegenerative disease. Identification and relative quantification of proteins affected by Prdx V may help identify novel signaling mechanisms that are important for oxidative stress response. However, the role of Prdx V in the modulation of hypoxia-related cellular response is not studied yet. To examine the function of endogenous Prdx V in hypoxic condition in vivo, we generated a transgenic mouse model with Prdx V siRNA expression controlled by U6 promoter. Of many tissues, the knockdown of Prdx V expression was displayed in the kidney, lung, and liver but not the spleen and skin. We conducted on the basis of nano-UPLC-MS(E) proteomic study to identify the Prdx V-affected protein networks in hypoxic kidneys. In this study, we identified protein networks associated with oxidative stress, fatty acid metabolism, and mitochondrial dysfunction. Our results indicated that Prdx V affected to regulation of kidney homeostasis under hypoxia stress.
authors.affiliation: Department of Oral Biochemistry, Dental Science Research Institute, The second Stage of Brain Korea 21 for Dental School, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Yang
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Cho
authors.forename: Hoon-In
authors.initials: HI
authors.lastname: Choi
authors.forename: Chiyoul
authors.initials: C
authors.lastname: Park
authors.forename: Hyang-Rim
authors.initials: HR
authors.lastname: Park
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Chung
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015687:Cell Hypoxia / Q000502:physiology*; D002853:Chromatography, Liquid; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D055785:Gene Knockdown Techniques; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000502:physiology*; D054464:Peroxiredoxins / Q000172:deficiency / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology*; D053719:Tandem Mass Spectrometry
chemical_list: D017931:DNA Primers; D054464:Peroxiredoxins