Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes.
In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7x10(7) cells/cm(3), being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture.
Kino-Oka
Masahiro
M
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kagita
Shogo
S
Nadzir
Masrina Mohd
MM
Inoue
Hirofumi
H
Sugawara
Katsura
K
Taya
Masahito
M
D000465:Algorithms; D000818:Animals; D002358:Cartilage, Articular; D002452:Cell Count / Q000379:methods*; D049109:Cell Proliferation; D019902:Chondrocytes / Q000502:physiology*; D003198:Computer Simulation; D008954:Models, Biological; D009924:Organ Culture Techniques; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits
D010100:Oxygen