Newcastle disease virus in penguins from King George Island on the Antarctic region.
Here we report the isolation of Newcastle disease virus (NDV) from cloacal swabs obtained from penguins in the South Atlantic Antarctic region (62°08S, 58°25W). Samples of 100 penguins from King George Island were tested by real-time PCR, of which 2 (2%) were positive for NDV. The positive samples were isolated in embryonated chicken eggs and their matrix and fusion proteins genes were partially sequenced. This was complemented by the serological study performed on the blood of the same specimens, which resulted in a 33.3% rate of positivity.
Thomazelli
Luciano M
LM
Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Araujo
Jansen
J
Oliveira
Danielle B
DB
Sanfilippo
Luiz
L
Ferreira
Carolina S
CS
Brentano
Liana
L
Pelizari
Vivian H
VH
Nakayama
Cristiane
C
Duarte
Rubens
R
Hurtado
Renata
R
Branco
Joaquim O
JO
Walker
David
D
Durigon
Edison L
EL
D000818:Animals; D000835:Animals, Wild / Q000821:virology; D000864:Antarctic Regions / Q000453:epidemiology; D001483:Base Sequence; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000453:epidemiology* / Q000821:virology; D009522:Newcastle disease virus / Q000302:isolation & purification*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D046429:Spheniscidae / Q000821:virology*