Three-dimensional scaffolds as a model system for neural and endothelial 'in vitro' culture.
Biomaterials based on the hydrophobic homopolymer poly(ethyl acrylate), PEA, and its copolymers with hydroxyethyl acrylate, p(EA-co-HEA) and methacrylic acid, p(EA-co-MAAc) were prepared as polymeric scaffolds with interconnected pores of 90 microns and tested in vitro as culture substrates and compared for their impact on the differentiation of neural stem cells (NSC) obtained from the subventricular zone (SVZ) of postnatal rats and human endothelial cells (HUVEC). Immunocytochemical staining assay for specific markers show that p(EA-co-MAAc) scaffolds were suitable substrates to promote cell attachment and differentiation of adult NSC and HUVEC cells.
Veiga
D Dias
DD
Center for Biomaterials and Tissue Engineering, Universidad Politécnica de Valencia, Camino de Vera, Spain.
Antunes
Joana Costa
JC
Gómez
Roberto García
RG
Mano
João F
JF
Ribelles
Jose Luis Gómez
JL
Soria
Jose Miguel
JM
D000179:Acrylates; D000180:Acrylic Resins; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D058953:Neural Stem Cells / Q000166:cytology*; D016062:Porosity; D051381:Rats; D046509:Tissue Culture Techniques / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D000179:Acrylates; D000180:Acrylic Resins; D001672:Biocompatible Materials; D008689:Methacrylates; C523542:poly(ethyl acrylate-co-hydroxyethyl acrylate); C523543:poly(ethyl acrylate-co-methacrylate); C478961:poly(ethylacrylate)