title: Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells.
abstract: Previous experimental studies to assess the contribution of blood-borne circulating (BBC) cells to cutaneous wound healing have relied on discontinuous pulsing of labeled BBC elements or bone marrow transplant protocols. Such approaches do not allow the examination of stable BBC cells that have matured in a physiologically normal host. We have used a parabiotic murine model for cutaneous wound healing to evaluate the relative contribution of stable populations of peripheral blood cells expressing the green fluorescent protein (GFP) transgene in otherwise normal animals. Circulating cells (mature and immature) expressing the GFP transgene were easily detected and quantified in wounds of GFP- parabiotic twins during all evaluated stages of the healing response. Using multiple antibody probes, the relative contribution of various subsets of BBC cells could be comparatively assessed. In early wounds, some cells expressing mesenchymal epitopes were documented to be of hematopoietic origin, indicating the utility of this model in assessing cell plasticity in the context of tissue regeneration and repair. Application of this approach enables further investigation into the contribution of peripheral blood in normal and abnormal healing responses.
authors.affiliation: Division of Plastic Surgery, Brigham & Women's Hospital, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Guodong
authors.initials: G
authors.lastname: Song
authors.forename: Dinh T
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pietramaggiori
authors.forename: Saja
authors.initials: S
authors.lastname: Scherer
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhan
authors.forename: Rei
authors.initials: R
authors.lastname: Ogawa
authors.forename: I V
authors.initials: IV
authors.lastname: Yannas
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Wagers
authors.forename: Dennis P
authors.initials: DP
authors.lastname: Orgill
authors.forename: George F
authors.initials: GF
authors.lastname: Murphy
mesh_terms: D000199:Actins / Q000502:physiology; D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D054338:Cell Transdifferentiation / Q000502:physiology; D024042:Collagen Type I / Q000502:physiology; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010227:Parabiosis / Q000379:methods; D019076:Transgenes / Q000502:physiology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000199:Actins; D015703:Antigens, CD; D024042:Collagen Type I; C544013:alpha-smooth muscle actin, mouse; D049452:Green Fluorescent Proteins