Two-dimensional liquid chromatography of diblock copolymers: simulation at various adsorption interaction conditions.
Diblock copolymers, which are heterogeneous in both molar mass and composition, can be fully characterized by using two-dimensional chromatography. Since the size-exclusion, the adsorption, and the critical interaction based modes of chromatography are possible for each of the polymers A and B, this leads to a variety of options for 2D-chromatography of copolymers AB. Using the theory of chromatography of block copolymers, 2D-chromatograms are simulated that correspond to the most interesting of these options. Orthogonal 2D-chromatograms are expected, if in the 1st dimension the critical condition is created for A, while in the 2nd dimension--for B. The situations, where A and B are both adsorbable, as well as those where the conditions of adsorption for A and SEC for B are created, are also considered. In particular, it is shown that the 2nd dimension combination of the critical condition for A and SEC--for B is preferable than that with SEC condition for both A and B. The simulated 2D-chromatograms of low- and high molar mass diblock copolymers, as well as of copolymers with one short block are compared with the reported real ones; it is concluded that the corresponding virtual and real 2D-chromatograms are qualitatively very similar.
Gorbunov
Alexei A
AA
Institute for Highly Pure Biopreparations, Lab. of Protein Biochemistry, 7 Pudozhskaya, 197110 St. Petersburg, Russia. alexeigorbunov@hotmail.com
Vakhrushev
Andrey V
AV
D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification*
D011108:Polymers