title: Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.
abstract: Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis.
authors.affiliation: Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35121 Padova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Cascioferro
authors.forename: Francesca
authors.initials: F
authors.lastname: Boldrin
authors.forename: Agnese
authors.initials: A
authors.lastname: Serafini
authors.forename: Roberta
authors.initials: R
authors.lastname: Provvedi
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
authors.forename: Riccardo
authors.initials: R
authors.lastname: Manganelli
mesh_terms: D005827:Genetics, Microbial / Q000379:methods*; D016254:Mutagenesis, Insertional / Q000379:methods*; D020102:Mycobacterium smegmatis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011995:Recombination, Genetic; D017384:Sequence Deletion