Overexpression of IGF-IR in malignant clonal cells in bone marrow of myelodysplastic syndromes.
OBJECTIVE
To explore the expression level of insulin-like growth factor-1 receptor (IGF-IR) in malignant clone cells of myelodysplastic syndromes (MDS).


METHODS
Fluorescence in situ hybridization (FISH) and immunochemistry (alkaline phosphatase antialkaline phosphatase) were used together to detect the expression of IGF-IR in the bone marrow cells of 26 MDS patients with known abnormal karyotypes.


RESULTS
The average IGF-IR expression level on the surface of clone cells from the 26 MDS cases was markedly elevated compared with the corresponding level in normal cells (78.2 ± 13.7% vs. 14.1 ± 14.0%, p < .0001). The percentages of malignant clone cells in all 26 MDS cases were significantly correlated with the respective percentages of IGF-IR-positive nucleated cells (r = 0.909; p < .0001). No significant difference in the IGF-IR expression level on the clone cells were observed either between high- and low-risk MDS patients or among MDS patients with different abnormal karyotypes.


CONCLUSIONS
IGF-IR might be taken as a marker of clone cells in MDS.
He
Qi
Q
Department of Hematology, Shanghai Sixth Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
Li
Xiao
X
Zhang
Zheng
Z
Zhang
Qianqiao
Q
Xu
Feng
F
Yang
Lianping
L
Tao
Ying
Y
Liu
Yizhi
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D002869:Chromosome Aberrations; D002999:Clone Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D055815:Young Adult
D017526:Receptor, IGF Type 1