Bentazon triggers the promotion of oxidative damage in the Portuguese ricefield cyanobacterium Anabaena cylindrica: response of the antioxidant system.
Rice fields are frequently exposed to environmental contamination by herbicides and cyanobacteria, as primary producers of these aquatic ecosystems, are adversely affected. Anabaena cylindrica is a cyanobacterium with a significantly widespread occurrence in Portuguese rice fields. This strain was studied throughout 72 h in laboratory conditions for its stress responses to sublethal concentrations (0.75-2 mM) of bentazon, a selective postemergence herbicide recommended for integrated weed management in rice, with special reference to oxidative stress, role of proline and intracellular antioxidant enzymes in herbicide-induced free radicals detoxification. Activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione S-transferase (GST) increased in a time- and herbicide dose-response manner and were higher than those in the control samples after 72 h. A time- and concentration-dependent increase of malondialdehyde (MDA) levels and the enhanced cell membrane leakage following bentazon exposure are indicative of lipid peroxidation, free radicals formation, and oxidative damage, while increased amounts of SOD, CAT, APX, GST, and proline indicated their involvement in free radical scavenging mechanisms. The appreciable decline in the reduced glutathione (GSH) pool after 72 h at higher bentazon concentrations could be explained by the reduction of the NADPH-dependent glutathione reductase (GR) activity. The obtained results suggested that the alterations of antioxidant systems in A. cylindrica might be useful biomarkers of bentazon exposure. As the toxic mechanism of bentazon is a complex phenomenon, this study also adds relevant findings to explain the oxidative stress pathways of bentazon promoting oxidative stress in cyanobacteria.
Galhano
Victor
V
Department of Biology and Environment/Centre for Research and Technology of Agro-Environment and Biological Sciences, University of Trás-os-Montes and Alto Douro, Apartado 1013 - 5001-801 Vila Real, Portugal. vgalhano@utad.pt
Peixoto
Francisco
F
Gomes-Laranjo
José
J
D046868:Anabaena cylindrica / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D001581:Benzothiadiazines / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D012275:Oryza / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011174:Portugal; D011392:Proline / Q000494:pharmacology; D012989:Soil Pollutants / Q000633:toxicity*; D013997:Time Factors
D000975:Antioxidants; D001581:Benzothiadiazines; D015415:Biomarkers; D005609:Free Radicals; D006540:Herbicides; D012989:Soil Pollutants; D011392:Proline; C002003:bentazone