Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Poly-gamma-glutamate (gamma-PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the gamma-PGA synthesis ywsC-ywtAB genes in its chromosome, cannot produce gamma-PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in gamma-PGA-producing B. subtilis mutant strains. Mutant B. subtilis PGA6-2 stably produces high levels of gamma-PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6-2 is not only a gamma-PGA producer, but it is also a candidate for the genetic and metabolic engineering of gamma-PGA production.
Yeh
Chuan-Mei
CM
Dept. of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, R.O. China. cmyeh@nchu.edu.tw
Wang
Jyh-Perng
JP
Lo
Shih-Ching
SC
Chan
Wen-Chia
WC
Lin
Ming-Yi
MY
D000643:Ammonium Chloride / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018698:Glutamic Acid / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
C511775:poly(gamma-glutamic acid); D000643:Ammonium Chloride; D011099:Polyglutamic Acid; D018698:Glutamic Acid