Cell tracking using nanoparticles.
Tracking cells in regenerative medicine is becoming increasingly important for basic cell therapy science, for cell delivery optimization and for accurate biodistribution studies. This report describes nanoparticles that utilize stable-isotope metal labels for multiple detection technologies in preclinical studies. Cells labeled with nanoparticles can be imaged by electron microscopy, fluorescence, and magnetic resonance. The nanoparticle-labeled cells can be quantified by neutron activation, thereby allowing, with the use of standard curves, the determination of the number of labeled cells in tissue samples from in vivo sources. This report describes the characteristics of these nanoparticles and methods for using these nanoparticles to label and track cells.
Vaccaro
Dennis E
DE
BioPhysics Assay Laboratory, Inc, 80 Webster St, Worcester, MA 01603, USA. dev@biopal.com
Yang
Meiheng
M
Weinberg
James S
JS
Reinhardt
Christopher P
CP
Groman
Ernest V
EV
D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D017690:Cell Transplantation; D005456:Fluorescent Dyes; D008279:Magnetic Resonance Imaging; D051379:Mice; D008854:Microscopy, Electron; D041681:NIH 3T3 Cells; D053758:Nanoparticles; D009501:Neutron Activation Analysis; D044968:Regenerative Medicine / Q000379:methods*; D012235:Rhodamines; D014057:Tomography, X-Ray Computed
D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B