title: [Preparation of a novel targeted MR contrast agent Gd-DTPA-Granzyme B monoclonal antibody].
abstract: To prepare a novel MRI targeted contrast agent Gd-DTPA-Granzyme B monoclonal antibody (mAb) and to test its reaction conditions.
  
  
  
  The Granzyme B mAb was coupled with DTPA,and then conjugated with Gd. The Gd-DTPA antibody was characterized using MALDI-TOF-MS. Cytotoxicity test was performed with MTT assay, and immune activation was examined with immunohistochemistry.
  
  
  
  MALDI-TOF-MS demonstrated that the molecular weight shifted from granzyme B mAb (133986) to Gd-DTPA-GB mAb (139736), which indicated the conjugation of the antibody with Gd-DTPA. The molar ratio of Gd per IgG molecule was about 20. MTT assay showed that Gd, DTPA, Gd-DTPA and Gd-DTPA-GB mAb groups did not make an impact on cell viability, and there were no significant differences among 4 groups (P>0.05). Immunohistochemistry results showed that compared with the positive control group the targeted contrast agent had a high immune activity.
  
  
  
  The novel contrast agent Gd-DTPA-Granzyme B mAb prepared in this study keeps a good immune activity and has no significant cytotoxicity.
authors.affiliation: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Guang-yi
authors.initials: GY
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: You-ying
authors.initials: YY
authors.lastname: Mao
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Jin
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry* / Q000633:toxicity; D019786:Gadolinium DTPA / Q000737:chemistry*; D053804:Granzymes / Q000276:immunology*; D008279:Magnetic Resonance Imaging; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D003287:Contrast Media; D053804:Granzymes; D019786:Gadolinium DTPA