Stable expression of rice dwarf virus Pns10 suppresses the post-transcriptional gene silencing in transgenic Nicotiana benthamiana plants.
RNA silencing is a conserved mechanism that defends against viral infection and retrotransposon activity for protection of the genome. Segment 10 (S10) of Rice dwarf virus (RDV) encodes Pns10 protein, a viral suppressor of RNAi that suppresses the host RNA silencing machinery. In this study, we obtained stable transgenic RDV-S10 Nicotiana benthamiana plants expressing Pns10. Suppression of post-transcriptional gene silencing (PTGS) by Pns10 supported the conclusion that this protein exhibited the RNA silencing suppressor activity. In particular, the transgenic plants stably expressing a viral suppressor of RNAi (VSR) provide a model system for investigating the mechanism of RNA silencing.<br />


BACKGROUND
RNA silencing; VSR; Rice dwarf virus; Pns10; transgenic plant.
Zhou
P
P
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, The National Plant Gene Research Center, Peking University, Beijing 100871, China.
Ren
B
B
Zhang
X M
XM
Wang
Y
Y
Wei
C H
CH
Li
Y
Y
D015870:Gene Expression; D005814:Genes, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics; D012275:Oryza / Q000821:virology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D011994:Recombinant Proteins / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins