Gqalpha-linked phospholipase Cbeta1 and phospholipase Cgamma are essential components of the pheromone biosynthesis activating neuropeptide (PBAN) signal transduction cascade.
Sex pheromone production for most moths is regulated by pheromone biosynthesis activating neuropeptide (PBAN). In Bombyx mori, PBAN binding triggers the opening of store-operated Ca(2+) channels, suggesting the involvement of a receptor-activated phospholipase C (PLC). In this study, we found that PLC inhibitors U73122 and compound 48/80 reduced sex pheromone production and that intracellular levels of (3)H-inositol phosphate species increased following PBAN stimulation. In addition, we amplified cDNAs from pheromone glands corresponding to PLCbeta1, PLCbeta4, PLCgamma and two G protein alpha subunits, Go and Gq. In vivo RNA interference-mediated knockdown analyses revealed that BmPLCbeta1, BmGq1, and unexpectedly, BmPLCgamma, are part of the PBAN signal transduction cascade.
Hull
J J
JJ
Molecular Entomology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan. Joe.Hull@ars.usda.gov
Lee
J M
JM
Matsumoto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005233:Fatty Alcohols / Q000378:metabolism; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008954:Models, Biological; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D010675:Pheromones / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000378:metabolism; D054799:Phospholipase C beta / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010802:Phylogeny; D021122:Protein Subunits / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects
D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D009479:Neuropeptides; D010675:Pheromones; D010716:Phosphatidylinositols; D021122:Protein Subunits; D012330:RNA, Double-Stranded; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; C006787:bombykol; D054799:Phospholipase C beta; D051966:Phospholipase C gamma; D043802:GTP-Binding Protein alpha Subunits, Gq-G11