title: A draft physical map of a D-genome cotton species (Gossypium raimondii).
abstract: BACKGROUND
  Genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. Cultivated tetraploid cottons, Gossypium hirsutum and G. barbadense, share a common ancestor formed by a merger of the A and D genomes about 1-2 million years ago. Toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the D genome progenitor Gossypium raimondii for complete sequencing.
  
  
  RESULTS
  A whole genome physical map of G. raimondii, the putative D genome ancestral species of tetraploid cottons was assembled, integrating genetically-anchored overgo hybridization probes, agarose based fingerprints and 'high information content fingerprinting' (HICF). A total of 13,662 BAC-end sequences and 2,828 DNA probes were used in genetically anchoring 1585 contigs to a cotton consensus genetic map, and 370 and 438 contigs, respectively to Arabidopsis thaliana (AT) and Vitis vinifera (VV) whole genome sequences.
  
  
  CONCLUSIONS
  Several lines of evidence suggest that the G. raimondii genome is comprised of two qualitatively different components. Much of the gene rich component is aligned to the Arabidopsis and Vitis vinifera genomes and shows promise for utilizing translational genomic approaches in understanding this important genome and its resident genes. The integrated genetic-physical map is of value both in assembling and validating a planned reference sequence.
authors.affiliation: Plant Genome Mapping Laboratory, University of Georgia, Athens, GA 30605, USA.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Lin
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Pierce
authors.forename: John E
authors.initials: JE
authors.lastname: Bowers
authors.forename: James C
authors.initials: JC
authors.lastname: Estill
authors.forename: Rosana O
authors.initials: RO
authors.lastname: Compton
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Rainville
authors.forename: Changsoo
authors.initials: C
authors.lastname: Kim
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lemke
authors.forename: Junkang
authors.initials: J
authors.lastname: Rong
authors.forename: Haibao
authors.initials: H
authors.lastname: Tang
authors.forename: Xiyin
authors.initials: X
authors.lastname: Wang
authors.forename: Michele
authors.initials: M
authors.lastname: Braidotti
authors.forename: Amy H
authors.initials: AH
authors.lastname: Chen
authors.forename: Kristen
authors.initials: K
authors.lastname: Chicola
authors.forename: Kristi
authors.initials: K
authors.lastname: Collura
authors.forename: Ethan
authors.initials: E
authors.lastname: Epps
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Golser
authors.forename: Corrinne
authors.initials: C
authors.lastname: Grover
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ingles
authors.forename: Santhosh
authors.initials: S
authors.lastname: Karunakaran
authors.forename: Dave
authors.initials: D
authors.lastname: Kudrna
authors.forename: Jaime
authors.initials: J
authors.lastname: Olive
authors.forename: Nabila
authors.initials: N
authors.lastname: Tabassum
authors.forename: Eareana
authors.initials: E
authors.lastname: Um
authors.forename: Marina
authors.initials: M
authors.lastname: Wissotski
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Andrea
authors.initials: A
authors.lastname: Zuccolo
authors.forename: Mehboob
authors.initials: M
authors.lastname: ur Rahman
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Peterson
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Wendel
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Paterson
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D016384:Consensus Sequence; D020451:Contig Mapping; D016172:DNA Fingerprinting; D019143:Evolution, Molecular; D020440:Gene Duplication; D017343:Genes, Plant / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D003368:Gossypium / Q000166:cytology / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D020161:Physical Chromosome Mapping / Q000379:methods*; D014176:Protein Biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D027843:Vitis / Q000235:genetics
chemical_list: D005819:Genetic Markers