[Preparation of a novel targeted MR contrast agent Gd-DTPA-Granzyme B monoclonal antibody].
To prepare a novel MRI targeted contrast agent Gd-DTPA-Granzyme B monoclonal antibody (mAb) and to test its reaction conditions.



The Granzyme B mAb was coupled with DTPA,and then conjugated with Gd. The Gd-DTPA antibody was characterized using MALDI-TOF-MS. Cytotoxicity test was performed with MTT assay, and immune activation was examined with immunohistochemistry.



MALDI-TOF-MS demonstrated that the molecular weight shifted from granzyme B mAb (133986) to Gd-DTPA-GB mAb (139736), which indicated the conjugation of the antibody with Gd-DTPA. The molar ratio of Gd per IgG molecule was about 20. MTT assay showed that Gd, DTPA, Gd-DTPA and Gd-DTPA-GB mAb groups did not make an impact on cell viability, and there were no significant differences among 4 groups (P>0.05). Immunohistochemistry results showed that compared with the positive control group the targeted contrast agent had a high immune activity.



The novel contrast agent Gd-DTPA-Granzyme B mAb prepared in this study keeps a good immune activity and has no significant cytotoxicity.
Xie
Yi
Y
Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Liu
Guang-yi
GY
Han
Fei
F
Jiang
Hong
H
Mao
You-ying
YY
Wang
Hui-ping
HP
Zhao
Jie
J
Jin
Juan
J
Chen
Jiang-hua
JH
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry* / Q000633:toxicity; D019786:Gadolinium DTPA / Q000737:chemistry*; D053804:Granzymes / Q000276:immunology*; D008279:Magnetic Resonance Imaging; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D053804:Granzymes; D019786:Gadolinium DTPA