Production of xylooligosaccharides from the steam explosion liquor of corncobs coupled with enzymatic hydrolysis using a thermostable xylanase.
The production of xylooligosaccharides (XOs) from corncobs was studied using a two-stage process based on a steam explosion pretreatment followed by enzymatic hydrolysis. Corncobs with different chip sizes were subjected to steam explosion under different experimental conditions of temperature and time, namely 188-204 degrees C for 2.5-7.5 min. The results indicate that corncobs were optimally steam exploded at 196 degrees C for 5 min, resulting in hemicellulose recovery of 22.8%. Especially, corncobs with large chip sizes (approximately 100 mm) during steam explosion pretreatment were suitable to produce XOs. Furthermore, a thermostable xylanase from Paecilomyces themophila J18 was used to hydrolyze steam explosion liquor of corncobs (SELC) for the production of XOs. A maximum XOs yield of 28.6 g XOs/100 g xylan in corncobs was achieved and XOs syrup contained more than 90% of xylobiose and xylotriose when the hydrolysis was carried out under the optimized conditions (pH 7.0, 70 degrees C, 7.5 U mL(-1) and 2.5 h). These results suggest that the process might be effective in production of XOs for industrial applications.
Teng
Chao
C
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Yan
Qiaojuan
Q
Jiang
Zhengqiang
Z
Fan
Guangsen
G
Shi
Bo
B
D002855:Chromatography, Thin Layer; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004795:Enzyme Stability; D006868:Hydrolysis; D009844:Oligosaccharides / Q000096:biosynthesis*; D013227:Steam; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
D009844:Oligosaccharides; D013227:Steam; D043364:Endo-1,4-beta Xylanases