title: Dextran gadolinium complexes as contrast agents for magnetic resonance imaging to sentinel lymph nodes.
abstract: OBJECTIVE
  The aim was to investigate three dextran gadolinium complexes Dextran-DTPA-Gd as the potential MRI contrast agents in lymphatic system.
  
  
  METHODS
  Three dextran gadolinium complexes Dextran-DTPA-Gd containing differing amounts of Gd-DTPA were synthesized by the incorporation of Gd-DTPA to the hydroxyl groups of dextran. These dextran ligands and gadolinium complexes were characterized, and their properties in vitro and in vivo were also evaluated.
  
  
  RESULTS
  Dextran-DTPA-Gd demonstrated obviously higher relaxation effectiveness than that of Gd-DTPA. The result of in vitro cytotoxicity assay showed that these macromolecular ligands and their corresponding gadolinium complexes had low cytotoxicity to HeLa cells. Dextran-DTPA-Gd greatly enhanced the contrast of MR images of normal politeal lymph nodes and reactive hyperplasia of politeal lymph nodes in rabbits and provided prolonged duration in lymphatic system with lower injection doses than that of Gd-DTPA. However, Dextran-DTPA-Gd displayed low signal enhancements in MR images of politeal lymph nodes with VX2 carcinoma in rabbits during the detection time.
  
  
  CONCLUSIONS
  These dextran gadolinium complexes Dextran-DTPA-Gd can be taken up selectively by lymphatic system and showed the potential as MRI contrast agents in lymphatic system.
authors.affiliation: School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan 430074, China. guopyan2006@163.com
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Lian
authors.initials: L
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.forename: Qing-Zhong
authors.initials: QZ
authors.lastname: Guo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D019786:Gadolinium DTPA / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D009374:Neoplasms, Experimental / Q000473:pathology; D010316:Particle Size; D011817:Rabbits
chemical_list: D003287:Contrast Media; D003911:Dextrans; C075783:dextran-(gadolinium-DTPA)15; D019786:Gadolinium DTPA