Probing oxidative stress in single erythrocytes with Raman Tweezers.
Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to SS and C-S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.
Zachariah
E
E
Centre for Atomic and Molecular Physics, Manipal University, Manipal 576 104, India.
Bankapur
A
A
Santhosh
C
C
Valiathan
M
M
Mathur
D
D
D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000494:pharmacology; D052898:Optical Tweezers; D018384:Oxidative Stress; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman
D017665:Hydroxyl Radical; D005978:Glutathione