title: Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma.
abstract: BACKGROUND
  Gliomas constitute the vast majority of primary central nervous system tumors in adults. Glioblastoma multiforme (GBM) is the most aggressive form of these primary brain tumors. There is a need to define diagnostic and prognostic markers that may help to distinguish GBM from non-GBM tumors. The Krüppel-like factor 6 (KLF6) gene has recently emerged as a promising candidate. The goal of our study was to determine if there is a link between KLF6 splice variants expression and different grades of gliomas.
  
  
  METHODS
  Fifty-three primary gliomas tumor samples were analyzed using quantitative real-time PCR for the total KLF6, wild-type and alternatively spliced (SV1) KLF6 mRNA.
  
  
  RESULTS
  Compared to the non-GBM group, the GBM group had a 2.2-fold increase in the mean level of total KLF6 mRNA expression. GBM showed a 2.1-fold increase in the KLF6 splicing ratio. In addition, KLF6-SV1 mRNA expression levels were also 2.2-fold higher in the GBM group, suggesting that the increase in the KLF6 splicing ratio was due to increased expression of the KLF6-SV1 oncogenic splice variant.
  
  
  CONCLUSIONS
  Our study demonstrates that quantification of total and spliced forms of KLF6 may provide a new and useful supplementary molecular tool for grading glioma.
authors.affiliation: University Clermont 1, EA 3846, School of Medicine, Clermont-Ferrand, France.
authors.forename: Andrei
authors.initials: A
authors.lastname: Tchirkov
authors.forename: Vincent
authors.initials: V
authors.lastname: Sapin
authors.forename: Geoffroy
authors.initials: G
authors.lastname: Marceau
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Chautard
authors.forename: Goutham
authors.initials: G
authors.lastname: Narla
authors.forename: Lauren
authors.initials: L
authors.lastname: Veronese
authors.forename: Scott
authors.initials: S
authors.lastname: Friedman
authors.forename: Toufic
authors.initials: T
authors.lastname: Khalil
authors.forename: Philippe
authors.initials: P
authors.lastname: Vago
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Kemeny
authors.forename: Pierre
authors.initials: P
authors.lastname: Verrelle
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics; D002273:Carcinogens; D005260:Female; D005786:Gene Expression Regulation; D005909:Glioblastoma / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002273:Carcinogens; C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger