title: Ex vivo cytotoxicity of a new calcium silicate-based canal filling material.
abstract: OBJECTIVE
  To evaluate the biocompatibility of iRoot SP root canal filling material to L929 mouse fibroblasts.
  
  
  METHODS
  Specimens of iRoot SP, AH Plus and ProRoot mineral trioxide aggregate (MTA) were prepared for direct contact using the Millipore filter diffusion test, as well as extracts in MTT assay. Mouse fibroblasts (L929) were used as toxicity targets. Differences in cytotoxicity between fresh and set specimens and between root canal material extracts were determined by t-test. A P-value <0.05 was considered statistically significant.
  
  
  RESULTS
  In the filter diffusion test, iRoot SP was slightly cytotoxic when tested as a fresh mixture and not cytotoxic in a set condition tested after 24 h. AH Plus was rated moderately cytotoxic when tested as a fresh mixture and slightly cytotoxic in a set (24 h) condition. MTA was noncytotoxic in both a fresh or set state. AH Plus was significantly more toxic than iRoot SP and MTA (P < 0.05). In addition, iRoot SP was significantly more toxic than MTA (P < 0.05). In the MTT assay with set specimens, extracts of iRoot SP and MTA were noncytotoxic, whilst extracts of AH Plus were rated as slightly cytotoxic. AH Plus was significantly more toxic than iRoot SP and MTA (P < 0.05).
  
  
  CONCLUSIONS
  AH Plus root canal sealer was significantly more toxic to L-929 cells than MTA and iRoot SP. iRoot SP had intermediate toxicity.
authors.affiliation: Key Lab for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Peng
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D017610:Calcium Compounds / Q000737:chemistry / Q000633:toxicity*; D018929:Cell Culture Techniques; D016923:Cell Death; D002460:Cell Line; D004396:Coloring Agents; D004058:Diffusion; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D008851:Micropore Filters; D010087:Oxides / Q000737:chemistry / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D017640:Silicates / Q000737:chemistry / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; D004853:Epoxy Resins; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C534916:epoxy resin-based root canal sealer; C000621519:iRoot SP; C086631:mineral trioxide aggregate; C022616:thiazolyl blue; C031293:calcium silicate