title: Comparative analysis of rabies virus reverse transcription-PCR and virus isolation using samples from a patient infected with rabies virus.
abstract: Definite and rapid diagnosis of rabies is required for individual case management as well as for public health. For the first time, a direct comparison of virus isolation with quantitative real-time reverse transcription (RT)-PCR on human rabies samples was conducted. RT-PCR was found to be more sensitive than virus isolation.
authors.affiliation: Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany. marcus.panning@uniklinik-freiburg.de
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Sigrid
authors.initials: S
authors.lastname: Baumgarte
authors.forename: Susanne
authors.initials: S
authors.lastname: Pfefferle
authors.forename: Tanja
authors.initials: T
authors.lastname: Maier
authors.forename: Andreas
authors.initials: A
authors.lastname: Martens
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
mesh_terms: D006801:Humans; D011818:Rabies / Q000175:diagnosis* / Q000821:virology; D011820:Rabies virus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods