title: Improved dsrA-based terminal restriction fragment length polymorphism analysis of sulfate-reducing bacteria.
abstract: To better describe the community structure of sulfate-reducing bacteria in environmental systems, we compared several dissimilatory sulfite reductase (dsr) primer sets for terminal restriction fragment length polymorphism application. A new reverse primer that increased allelic diversity estimates up to 5-fold was applied to hydrocarbon seep samples to examine the relationship between guild activity and diversity.
authors.affiliation: Microbial Habitat Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Santillano
authors.forename: Antje
authors.initials: A
authors.lastname: Boetius
authors.forename: Alban
authors.initials: A
authors.lastname: Ramette
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004783:Environmental Microbiology; D010084:Oxidation-Reduction; D012150:Polymorphism, Restriction Fragment Length; D013431:Sulfates / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D013431:Sulfates