title: Protective efficacy of a Classical swine fever virus C-strain deletion mutant and ability to differentiate infected from vaccinated animals.
abstract: Classical swine fever (CSF) continues to be the most economically damaging pig disease in the world. The disease can be effectively controlled by vaccination with the live C-strain vaccine. This vaccine, however, does not enable the serological differentiation between infected and vaccinated animals (DIVA) and its use can therefore impose severe trade restrictions. CSF-specific diagnostic ELISAs detect antibodies directed against the conserved and immunodominant A domain of the E2 structural glycoprotein. We previously reported the production of a C-strain virus in which the immunodominant TAVSPTTLR epitope of the A domain is stably mutated with the aim to render the virus suitable as a DIVA vaccine. We here report that a single vaccination with this vaccine virus protected pigs from a lethal challenge dose of the highly virulent Brescia strain. Analysis of the sera, however, demonstrated that a commercially available E2 ELISA was unsuitable as an accompanying DIVA test.
authors.affiliation: Virology Division, Central Veterinary Institute of Wageningen University Research Center, P.O. Box 65, 8200 AB Lelystad, The Netherlands. jeroen.kortekaas@wur.nl
authors.forename: J
authors.initials: J
authors.lastname: Kortekaas
authors.forename: J
authors.initials: J
authors.lastname: Ketelaar
authors.forename: R P M
authors.initials: RP
authors.lastname: Vloet
authors.forename: W L
authors.initials: WL
authors.lastname: Loeffen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000276:immunology* / Q000503:physiopathology / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D017384:Sequence Deletion; D013552:Swine; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus