A new method for designing degenerate primers and its use in the identification of sequences in Brachiaria showing similarity to apomixis-associated genes.
We developed a technique and a tool for degenerate primer design based on multiple local alignments employing the MEME algorithm supported with electronic PCR. The objective is to find adequate primers starting from sequences with poor global similarity. We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.
Gorrón
Eduardo
E
Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia.
Rodríguez
Fausto
F
Bernal
Diana
D
Rodriguez-Rojas
Luis Miguel
LM
Bernal
Adriana
A
Restrepo
Silvia
S
Tohme
Joe
J
D000465:Algorithms; D001483:Base Sequence; D035201:Brachiaria / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry*; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA
D017931:DNA Primers