title: Rab and actomyosin-dependent fission of transport vesicles at the Golgi complex.
abstract: Trafficking between membrane compartments is a characteristic of eukaryotic cells and relies on transport carriers that bud and fission from a donor membrane, before being transported and fusing with the correct acceptor compartment. Rab GTPases ensure specificity and directionality of trafficking steps by regulating the movement of transport carriers along cytoskeletal tracks, and the recruitment of tethering factors required for the docking and fusion processes. Here we show that Rab6, a Golgi-associated Rab, forms a complex with myosin II, contributes to its localization at the Golgi complex and, unexpectedly, controls the fission of Rab6 vesicles. Inhibition of either Rab6 or myosin II function impairs both the fission of Rab6 transport carriers from Golgi membranes and the trafficking of anterograde and retrograde cargo from the Golgi. These effects are consistent with myosin II being an effector of Rab6 in these processes. Our results provide evidence that the actomyosin system is required in vesicle biogenesis at the Golgi, and uncover a function for Rab GTPases in vesicle fission.
authors.affiliation: Institut Curie, CNRS UMR144, Molecular mechanisms of intracellular transport Lab, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: S
authors.initials: S
authors.lastname: Miserey-Lenkei
authors.forename: G
authors.initials: G
authors.lastname: Chalancon
authors.forename: S
authors.initials: S
authors.lastname: Bardin
authors.forename: E
authors.initials: E
authors.lastname: Formstecher
authors.forename: B
authors.initials: B
authors.lastname: Goud
authors.forename: A
authors.initials: A
authors.lastname: Echard
mesh_terms: D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008954:Models, Biological; D024462:Myosin Type II / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D000199:Actins; C094135:Rab6 protein; D000205:Actomyosin; D024462:Myosin Type II; D020691:rab GTP-Binding Proteins