Intestinal ferroportin expression in pediatric Crohn's disease.
BACKGROUND
Anemia is a frequent complication of Crohn's disease (CD). The intestinal iron exporter ferroportin (FPN) is involved in both iron deficiency anemia and the anemia of chronic disease. To examine its role in CD, intestinal FPN expression was studied in subjects with and without CD.


METHODS
Duodenal mucosal biopsies from 29 pediatric subjects with CD (n=19) and without CD (n=10) were obtained. FPN protein was measured using Western blot analysis and mRNA was assessed using quantitative real-time polymerase chain reaction (PCR).


RESULTS
Intestinal FPN protein was higher in anemic CD subjects than in nonanemic CD subjects (P=0.01), while FPN mRNA levels were not different (P=0.66). In nonanemic CD subjects, erythrocyte sedimentation rate (ESR) (P=0.04), C-reactive protein (CRP) (P=0.03), and interleukin-6 (IL-6) (P=0.01) levels were elevated compared to controls. Nonanemic CD subjects had a lower median FPN protein than nonanemic controls, although it did not reach statistical significance (P=0.07). Median FPN mRNA was similar between groups (P=0.71). Although no correlation between FPN protein and IL-6 was noted, there was a strong negative correlation between serum iron and IL-6, both in subjects with CD (r=-0.88, P<0.0001) and those without anemia (r=-0.58, P=0.02).


CONCLUSIONS
Intestinal FPN protein is upregulated in anemic CD subjects, suggesting that iron deficiency or anemia is the driving force regulating FPN levels. A transporter distinct from FPN appears to be involved in the hypoferremia associated with the inflammatory process of CD.
Burpee
Tyler
T
Center for Inflammatory Bowel Disease and Division of Gastroenterology and Nutrition, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA. tyler.burpee@seattlechildrens.org
Mitchell
Paul
P
Fishman
Douglas
D
Islam
Shabana
S
Nemeth
Elizabeta
E
Westerman
Mark
M
Wessling-Resnick
Marianne
M
Grand
Richard J
RJ
D000293:Adolescent; D000328:Adult; D018798:Anemia, Iron-Deficiency / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D003424:Crohn Disease / Q000150:complications / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D000098742:Ferroportin
D027682:Cation Transport Proteins; D012333:RNA, Messenger; D000098742:Ferroportin; D002097:C-Reactive Protein