Purified c-phycoerythrin: safety studies in rats and protective role against permanganate-mediated fibroblast-DNA damage.
We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C-PE) on three human cell lines by cell proliferation and neutral red uptake assays. No toxic effects of C-PE were observed to any of the cell lines tested. The protective role of purified C-PE to potassium permanganate-mediated human fibroblast-DNA damage was assessed by comet assay at 0 (control), 10 and 20 microg C-PE ml(-1) doses in pre-, simultaneous and post-mutagen exposure conditions. Significant DNA damage was detected only in post-mutagen exposure conditions. Our findings confirmed that the C-PE is non-toxic and provides protection against permanganate-mediated DNA damage. The preliminary acute (2000 mg C-PE kg(-1) body weight, b.w.) and 90 day sub-chronic (0, 5, 15 and 25 mg C-PE kg(-1) b.w./day) oral toxicity studies of purified C-PE in male albino rats showed no mortality or treatment-related major clinical signs, and all the doses of C-PE were well tolerated. The no observed adverse effect level and no observed effect level were found to be 15 and 5 mg C-PE kg(-1) b.w./day respectively.
Soni
Badrish
B
Laboratoire de Biologie Intégrative, CEA Saclay, 91191 Gif Sur Yvette, France. sonibadrish@gmail.com
Visavadiya
Nishant P
NP
Dalwadi
Nirav
N
Madamwar
Datta
D
Winder
Chris
C
Khalil
Christian
C
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival; D002478:Cells, Cultured; D020552:Comet Assay; D000458:Cyanobacteria / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D008297:Male; D010799:Phycoerythrin / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity*; D011196:Potassium Permanganate; D051381:Rats; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
D000975:Antioxidants; D011196:Potassium Permanganate; D010799:Phycoerythrin