Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation.
Heterochromatin protein 1 (HP1) has an essential role in heterochromatin formation and mitotic progression through its interaction with various proteins. We have identified a unique HP1alpha-binding protein, POGZ (pogo transposable element-derived protein with zinc finger domain), using an advanced proteomics approach. Proteins generally interact with HP1 through a PxVxL (where x is any amino-acid residue) motif; however, POGZ was found to bind to HP1alpha through a zinc-finger-like motif. Binding by POGZ, mediated through its zinc-finger-like motif, competed with PxVxL proteins and destabilized the HP1alpha-chromatin interaction. Depletion experiments confirmed that the POGZ HP1-binding domain is essential for normal mitotic progression and dissociation of HP1alpha from mitotic chromosome arms. Furthermore, POGZ is required for the correct activation and dissociation of Aurora B kinase from chromosome arms during M phase. These results reveal POGZ as an essential protein that links HP1alpha dissociation with Aurora B kinase activation during mitosis.
Nozawa
Ryu-Suke
RS
Graduate School of Life Science, Hokkaido University, Sapporo 001-0021, Japan.
Nagao
Koji
K
Masuda
Hiro-Taka
HT
Iwasaki
Osamu
O
Hirota
Toru
T
Nozaki
Naohito
N
Kimura
Hiroshi
H
Obuse
Chikashi
C
D064107:Aurora Kinase B; D064127:Aurora Kinases; D015153:Blotting, Western; D002460:Cell Line; D002842:Chromatids / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D018386:Kinetochores / Q000378:metabolism; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D034622:RNA Interference; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D004268:DNA-Binding Proteins; D018797:Cell Cycle Proteins
D064107:Aurora Kinase B; D064127:Aurora Kinases; D002843:Chromatin; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone; D017346:Protein Serine-Threonine Kinases; D019895:Transposases; C000715594:CBX5 protein, human; C541037:POGZ protein, human; D004268:DNA-Binding Proteins; D018797:Cell Cycle Proteins; C576921:AURKB protein, human