title: Real-time monitoring of copper ions-induced cytotoxicity by EIS cell chips.
abstract: An important goal of biomedical research is the development of tools for high-throughput evaluation of drug effects and cytotoxicity tests. Here we demonstrate EIS cell chips able to monitor cell growth, morphology, adhesion and their changes as a consequence of treatment with drugs or toxic compounds. As a case study, we investigate the uptake of copper ions and its effect on two cell lines: B104 and HeLa cells. For further understanding, we also carried out in parallel with EIS studies, a complete characterization of cell morphology and changes induced by copper ions through complementary methodologies (including state-of-the-art AFM, viability test and Western blot). Our results reveal a strong correlation between EIS data and both MTT test and AFM characterization so our chip can be used as powerful tools in all biology lab in combination with other standard methods giving additional information that can be useful in a complete and deep investigation of a biological process. This chip can be used even alone replacing in vitro drug tests based on conventional biochemical methods, being very cheap and reusable and allowing to perform cytotoxicity tests without using any expensive reagent or equipment.
authors.affiliation: Scuola Superiore ISUFI, University of Salento, CNR-Institute of Nanoscience, Lecce, Italy. elisabetta.primiceri@unisalento.it
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Chiriacò
authors.forename: Eliana
authors.initials: E
authors.lastname: D'Amone
authors.forename: Emanuela
authors.initials: E
authors.lastname: Urso
authors.forename: Rodica Elena
authors.initials: RE
authors.lastname: Ionescu
authors.forename: Antonia
authors.initials: A
authors.lastname: Rizzello
authors.forename: Michele
authors.initials: M
authors.lastname: Maffia
authors.forename: Roberto
authors.initials: R
authors.lastname: Cingolani
authors.forename: Ross
authors.initials: R
authors.lastname: Rinaldi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D017097:Electric Impedance; D006367:HeLa Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D018621:PrPC Proteins / Q000378:metabolism; D051381:Rats; D013057:Spectrum Analysis / Q000295:instrumentation*
chemical_list: D018621:PrPC Proteins; D003300:Copper