[Preparation of monolithic polylaurylmethacrylate column and its application in capillary electrochromatographic separation of myoglobin digests].
The monolithic polylaurylmethacrylate column was prepared in a single step using the monomer solution containing lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA), and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), with the ternary porogenic solvent consisting of 1-propanol, 1,4-butanediol and H2O. The effects of AMPS content and the ratio of monomer solution to porogenic solvent were investigated. The optimal mass ratio of monomer solution to porogenic solvent was 35:65, the monomer solution was composed of 59.5% (w/w) LMA, 40% (w/w) EDMA and 0.5% (w/w) AMPS, and the porogenic solution was composed of 60% (w/w) 1-propanol, 30% (w/w) 1,4-butanediol and 10% (w/w) H2O. The prepared monolithic column was successfully applied in the capillary electrochromatographic (CEC) separation of myoglobin digests under the optimized mobile phase.
Wang
Tingting
T
National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Liang
Zhen
Z
Zhang
Lihua
L
Zhang
Yukui
Y
D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D009211:Myoglobin / Q000302:isolation & purification* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis
D008689:Methacrylates; D009211:Myoglobin; D011108:Polymers; C115846:dodecyl methacrylate