High-throughput profiling of the humoral immune responses against thirteen human papillomavirus types by proteome microarrays.
We have developed microarrays with all eight proteins encoded by 13 different human papillomavirus types associated with anogenital cancer (HPV-16, -18, -31, -33, -35, -45, and -53), genital warts (HPV-6 and -11), or skin lesions (HPV-1, -2, -4, and -5). We analyzed the seroprevalence of antibodies in 546 patients, which had either cervical carcinomas, or precursor lesions, or which were asymptomatic. All patient groups contained sera ranging from high reactivity against multiple HPV proteins to low or no reactivity. Computational analyses showed the E7 proteins of carcinogenic HPV types as significantly more reactive in cancer patients compared to asymptomatic individuals and discriminating between cancer and HSIL or LSIL patients. Antibodies against E4 and E5 had the highest seroprevalence but did not exhibit differential reactivity relative to pathology. Our study introduces a new approach to future evaluation of the overall antigenicity of HPV proteins and cross-reaction between homologous proteins.
Luevano
Martha
M
Departamento de Bioquimica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.
Bernard
Hans-Ulrich
HU
Barrera-Saldaña
Hugo A
HA
Trevino
Victor
V
Garcia-Carranca
Alejandro
A
Villa
Luisa L
LL
Monk
Bradley J
BJ
Tan
Xiaolin
X
Davies
D Huw
DH
Felgner
Phil L
PL
Kalantari
Mina
M
D000230:Adenocarcinoma / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood*; D002675:Child, Preschool; D005260:Female; D016679:Genome, Viral; D006801:Humans; D018307:Neoplasms, Squamous Cell / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*; D040081:Protein Array Analysis; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000276:immunology / Q000821:virology
D000914:Antibodies, Viral