Systematic position of Histomonas meleagridis based on four protein genes.
Phylogenetic trees based on parabasalid sequences of the small subunit rRNA placed Histomonas meleagridis in close proximity to Dientamoeba fragilis, Tritrichomonas foetus, and Monocercomonas sp. In this study, we sequenced partial genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), enolase, and alpha-tubulin from 2 strains of H. meleagridis. We found 5 different GAPDH sequences, 6 different enolase sequences, and 3 alpha-tubulin sequences. Phylogenetic trees based on the obtained sequences showed a close relationship of H. meleagridis with T. foetus and, to some extent, Monocercomonas sp. In conclusion, our findings further corroborate the ssu rRNA-based tree. Consequently, our study also supports the hypothesis that H. meleagridis secondarily lost cytoskeletal structures.
Hauck
Rüdiger
R
Institute of Poultry Diseases, Faculty of Veterinary Medicine, Free University Berlin, Berlin, Germany. hauck.ruediger@vetmed.fu-berlin.de
Hafez
Hafez M
HM
D000595:Amino Acid Sequence; D001483:Base Sequence; D001499:Bayes Theorem; D016384:Consensus Sequence; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000235:genetics*; D016013:Likelihood Functions; D056900:Parabasalidea / Q000737:chemistry / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000737:chemistry / Q000235:genetics*
D015800:Protozoan Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014404:Tubulin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010751:Phosphopyruvate Hydratase