title: Assessing the effectiveness of intubation as a challenge model in contagious bovine pleuropneumonia vaccine experiments.
abstract: A study was carried out to assess the effectiveness of a bronchoscope in administering a pathogenic field strain of Mycoplasma mycoides subsp. mycoides (MmmSC) in cattle challenge experiments. Out of 16 animals inoculated using the bronchoscope, 10 (62.2%) showed clinical disease as evidenced by fever and 15 (93.8%) displayed typical lesions of CBPP from which MmmSC was isolated. Serum samples collected weekly were tested by Complement Fixation Test (CFT) and competitive enzyme-linked immunosorbent assay (c-ELISA). Antibodies to MmmSC were detected in 10 out of the 16 animals by the CFT and 11 out of the 16 animals by c-ELISA. The onset of clinical disease was as early as 2 days post-inoculation, and most of the animals developed clinical disease 2 to 3 weeks post-infection. These results clearly demonstrate that nasotracheal inoculation of pathogenic strain of MmmSC with the aid of a bronchoscope can lead to early onset of clinical disease; similar to previous studies but with higher numbers of animals showing clinical disease. This is in contrast with previous studies where early clinical disease was observed in as little as 15% of inoculated animals. This nasotracheal inoculation method using a bronchoscope can, therefore, be adopted for use in experimental challenge infections of cattle. This method is found to be a better replacement to the contact transmission method whose drawback includes extra cost of donor animals and unpredictable rate and timing of transmission from intubated to challenge animals.
authors.affiliation: Veterinary Research Centre, Kenya Agricultural Research Institute, P.O. Box 32-00902, Kikuyu, Kenya. docgacherinkando@yahoo.co.uk
authors.forename: Isabel Gacheri
authors.initials: IG
authors.lastname: Nkando
authors.forename: Hezron O
authors.initials: HO
authors.lastname: Wesonga
authors.forename: Joseph K N
authors.initials: JK
authors.lastname: Kuria
authors.forename: Declan
authors.initials: D
authors.lastname: McKeever
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001985:Bronchial Provocation Tests / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D020680:Bronchoscopes / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007442:Intubation, Intratracheal / Q000379:methods / Q000662:veterinary*; D009176:Mycoplasma mycoides / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000382:microbiology*
chemical_list: D000907:Antibodies, Bacterial