Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish.
The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.
Francisco-Simão
M
M
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
Cardona-Costa
J
J
Pérez-Camps
M
M
García-Ximénez
F
F
D000818:Animals; D046528:Chimerism / Q000187:drug effects* / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000528:radiation effects; D009994:Osmolar Concentration; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000196:embryology*