Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.).
Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.
Pirseyedi
Seyed Mostafa
SM
Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran; E-Mails: m_pirseyedi@abrii.ac.ir (S.M.P.); s.valizadegan@gmail.com (S.V.); ghaffary@abrii.ac.ir (M.R.G.); mparvaneh59@yahoo.com (P.M.); mzeinolabedini@abrii.ac.ir (M..Z.); khayam@abrii.ac.ir (S.M.K.N.).
Valizadehghan
Sahar
S
Mardi
Mohsen
M
Ghaffari
Mohammad Reza
MR
Mahmoodi
Parvaneh
P
Zahravi
Mehdi
M
Zeinalabedini
Mehrshad
M
Nekoui
Seyed Mojtaba Khayam
SM
D000483:Alleles; D056426:Genetic Loci; D015698:Genomic Library; D029561:Lythraceae / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic