Observations of the mechanisms of electromediated DNA uptake--from vesicles to tissues.
We discuss experimental observations of DNA uptake induced by electropermeabilisation. First we describe how experiments on giant unilamelar vesicles can be used to understand the effect of electric fields on lipid membranes and the associated transfer of DNA across the plasma membrane. We then discuss how DNA interacts with electropermeabilised cells in culture and how gene expression is correlated to observations of interactions between DNA and the cell membrane. Finally we discuss how DNA uptake occurs and can be optimized for the most medically relevant case of tissues.
Golzio
Muriel
M
CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077 Toulouse, France.
Escoffre
Jean-Michel
JM
Portet
Thomas
T
Mauroy
Chloé
C
Teissié
Justin
J
Dean
David S
DS
Rols
Marie-Pierre
MP
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D002463:Cell Membrane Permeability; D004247:DNA / Q000378:metabolism*; D004574:Electromagnetic Fields; D018274:Electroporation; D015870:Gene Expression / Q000502:physiology; D018014:Gene Transfer Techniques; D051379:Mice; D053835:Unilamellar Liposomes / Q000378:metabolism
D053835:Unilamellar Liposomes; D004247:DNA