title: Gqalpha-linked phospholipase Cbeta1 and phospholipase Cgamma are essential components of the pheromone biosynthesis activating neuropeptide (PBAN) signal transduction cascade.
abstract: Sex pheromone production for most moths is regulated by pheromone biosynthesis activating neuropeptide (PBAN). In Bombyx mori, PBAN binding triggers the opening of store-operated Ca(2+) channels, suggesting the involvement of a receptor-activated phospholipase C (PLC). In this study, we found that PLC inhibitors U73122 and compound 48/80 reduced sex pheromone production and that intracellular levels of (3)H-inositol phosphate species increased following PBAN stimulation. In addition, we amplified cDNAs from pheromone glands corresponding to PLCbeta1, PLCbeta4, PLCgamma and two G protein alpha subunits, Go and Gq. In vivo RNA interference-mediated knockdown analyses revealed that BmPLCbeta1, BmGq1, and unexpectedly, BmPLCgamma, are part of the PBAN signal transduction cascade.
authors.affiliation: Molecular Entomology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan. Joe.Hull@ars.usda.gov
authors.forename: J J
authors.initials: JJ
authors.lastname: Hull
authors.forename: J M
authors.initials: JM
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005233:Fatty Alcohols / Q000378:metabolism; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008954:Models, Biological; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D010675:Pheromones / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000378:metabolism; D054799:Phospholipase C beta / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051966:Phospholipase C gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010802:Phylogeny; D021122:Protein Subunits / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D009479:Neuropeptides; D010675:Pheromones; D010716:Phosphatidylinositols; D021122:Protein Subunits; D012330:RNA, Double-Stranded; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; C006787:bombykol; D054799:Phospholipase C beta; D051966:Phospholipase C gamma; D043802:GTP-Binding Protein alpha Subunits, Gq-G11