PhiC31 integrase induces efficient site-specific excision in zebrafish.
Site-specific recombinases catalyze recombination between specific targeting sites to delete, insert, invert, or exchange DNA with high fidelity. In addition to the widely used Cre and Flp recombinases, the phiC31 integrase system from Streptomyces phage may also be used for these genetic manipulations in eukaryotic cells. Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish. Here we show that phiC31 integrase efficiently induces site-specific deletion of episomal targets as well as chromosomal targets in zebrafish embryos. Thus, the phiC31 system can be used in zebrafish for genetic manipulations, expanding the repertoire of available tools for genetic manipulation in this vertebrate model.
Lu
Jianjun
J
Vollum Institute, OHSU, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA.
Maddison
Lisette A
LA
Chen
Wenbiao
W
D000818:Animals; D030801:Animals, Genetically Modified; D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000201:enzymology* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D011995:Recombination, Genetic; D013302:Streptomyces / Q000821:virology; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism / Q000821:virology
D019426:Integrases