Direct detection of Mycoplasma bovis in milk and tissue samples by real-time PCR.
Bovine mastitis caused by Mycoplasma bovis is of great economic importance to the beef and dairy industry. Here we describe a new specific real-time PCR assay targeting the uvrC gene that was developed to directly detect M. bovis from milk and tissue samples without laborious DNA purification.
Rossetti
Bigna C
BC
Institute for Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Länggassstrasse 122, 3012 Bern, Switzerland. bigna.rossetti@vbi.unibe.ch
Frey
Joachim
J
Pilo
Paola
P
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000235:genetics; D005260:Female; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology* / Q000662:veterinary; D045682:Mycoplasma bovis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D017931:DNA Primers; D004706:Endodeoxyribonucleases