A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells.
MicroRNAs are approximately 22nt non-coding RNAs that are present in a broad range of multicellular organisms. MicroRNAs play important roles in many biological or pathological processes by regulating the expression of their target genes. The fast and accurate identification of miRNA targets is a bottleneck in the clarification of the function of miRNAs. Here, we established a rapid and accurate strategy to identify miRNA functional target genes by combination of bioinformatic prediction with Cytoplasmic/Nuclear (C/N) ratios of mRNAs. The strategy comprises three steps: bioinformatic prediction, determination of mRNA C/N ratios, and confirmation by Western blotting, and might be suitable to most miRNAs. Our method will make a significant contribution to the study of the biological functions of miRNAs.
Li
Jie
J
Beijing Institute of Basic Medical Sciences, Beijing, China.
Xia
Wei
W
Huang
Baochun
B
Chen
Liucun
L
Su
Xueting
X
Li
Shaohua
S
Wang
Fang
F
Ding
Hongmei
H
Shao
Ningsheng
N
D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002477:Cells / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
D035683:MicroRNAs; D012333:RNA, Messenger