title: KRAS, BRAF, EGFR and HER2 gene status in a Spanish population of colorectal cancer.
abstract: To evaluate the KRAS, BRAF, EGFR, and HER2 gene status in colorectal cancer by novel techniques and evaluate whether anti-HER2 therapies could be offered in the treatment of these patients. There are conflicting data on the prevalence of BRAF mutations and EGFR and HER2 gene amplification in colorectal KRAS wild type patients. In our study we tried to evaluate these expressions and their relationship to future treatment assays. Clinical-pathological data and paraffin-embedded specimens were collected from 186 patients who underwent colorectal resections at General Yagüe Hospital in Burgos, Spain. KRAS and BRAF status was analyzed by real-time PCR in all patients. EGFR and HER2/NEU gene amplification was detected using fluorescent in situ hybridisation technique (FISH) in 38 KRAS and BRAF wild type patients. KRAS mutations were present in 48% of the colorectal cancer patients. BRAF mutations were present in 6.25% of the KRAS wild type patients. EGFR and HER2 gene amplification was observed in 5.3% and 26.3%, respectively, of KRAS and BRAF wild type colorectal cancer patients. HER2, but not EGFR gene amplification, was frequently observed in KRAS and BRAF wild type colorectal cancer patients. These data indicate that HER2 amplification could be one of the genes to be considered in the therapeutic management of colorectal cancer.
authors.affiliation: Unidad de Investigación, Hospital General Yagüe, Avenida del Cid 96, 09005, Burgos, Spain. mhv@hgy.es
authors.forename: Marta
authors.initials: M
authors.lastname: Herreros-Villanueva
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Rodrigo
authors.forename: Manuel
authors.initials: M
authors.lastname: Claver
authors.forename: Pilar
authors.initials: P
authors.lastname: Muñiz
authors.forename: Enrique
authors.initials: E
authors.lastname: Lastra
authors.forename: Carlos
authors.initials: C
authors.lastname: García-Girón
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Coma del Corral
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000208:ethnology* / Q000235:genetics*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000096:biosynthesis*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D012189:Retrospective Studies; D013030:Spain
chemical_list: D066246:ErbB Receptors; D048493:Proto-Oncogene Proteins B-raf; D016283:Proto-Oncogene Proteins p21(ras); D018719:Erb-b2 Receptor Tyrosine Kinases; C482119:BRAF protein, human