Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death.
Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.
Gupta
Anurag Kumar
AK
University Women's Hospital, Department of Biomedicine, Basel University Hospital, Basel, Switzerland.
Joshi
Manjunath B
MB
Philippova
Maria
M
Erne
Paul
P
Hasler
Paul
P
Hahn
Sinuhe
S
Resink
Therese J
TJ
D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology; D004727:Endothelium / Q000187:drug effects / Q000276:immunology; D005110:Extracellular Space / Q000187:drug effects / Q000276:immunology*; D005260:Female; D056660:Hereditary Autoinflammatory Diseases; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000502:physiology; D011225:Pre-Eclampsia / Q000276:immunology; D011247:Pregnancy
D016209:Interleukin-8