[Experimental foundation of using intraoperative endopelvic cisplatin with hyperthermia for rectal cancer].
Previously, significant increase in survival in locally-advanced rectal cancer as a result of heated intraoperative intraperitoneal chemotherapy was reported. Our study used cisplatin 0.5 mg/ml (0.05 per cent solution) in the culture of pharyngeal epidermoid carcinoma (PEC) cells (HEP-2) and A-549 culture of lung carcinoma cells. The number of viable cells was estimated colorimetrically after 24 and 48 hr incubation. 50%-rise in inhibition of culture growth was assumed to be biologically significant. Forty-eight hours after inoculation, single dose of cisplatin 8 mg/kg was injected in mice bearing transplanted lung carcinoma of Lewis (LLC). That was followed by death of tumor cells. Preheating (45 deg. C, 1 hr) did not influence either the cytostatic or therapeutic effect of cisplatin in vivo.That procedure inhibited tumor growth by 7-8% and the effect did not wear off until day 11 or longer. Survival in LLC-bearing mice rose by 26% which pointed to the advantages offered by heated cytostatic chemotherapy.
Shelygin
Iu A
IuA
Alekseev
M V
MV
Rybakov
E G
EG
Pankratov
A A
AA
Pliushinskaia
A D
AD
D000230:Adenocarcinoma / Q000628:therapy; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000628:therapy; D002294:Carcinoma, Squamous Cell / Q000628:therapy; D045744:Cell Line, Tumor; D017024:Chemotherapy, Adjuvant; D002945:Cisplatin / Q000008:administration & dosage / Q000627:therapeutic use*; D003124:Colorimetry; D004334:Drug Administration Schedule; D006801:Humans; D006979:Hyperthermia, Induced; D007432:Intraoperative Period; D008175:Lung Neoplasms / Q000628:therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010610:Pharyngeal Neoplasms / Q000628:therapy; D012004:Rectal Neoplasms / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D013997:Time Factors
D000970:Antineoplastic Agents; D002945:Cisplatin