title: Systems microscopy approaches to understand cancer cell migration and metastasis.
abstract: Cell migration is essential in a number of processes, including wound healing, angiogenesis and cancer metastasis. Especially, invasion of cancer cells in the surrounding tissue is a crucial step that requires increased cell motility. Cell migration is a well-orchestrated process that involves the continuous formation and disassembly of matrix adhesions. Those structural anchor points interact with the extra-cellular matrix and also participate in adhesion-dependent signalling. Although these processes are essential for cancer metastasis, little is known about the molecular mechanisms that regulate adhesion dynamics during tumour cell migration. In this review, we provide an overview of recent advanced imaging strategies together with quantitative image analysis that can be implemented to understand the dynamics of matrix adhesions and its molecular components in relation to tumour cell migration. This dynamic cell imaging together with multiparametric image analysis will help in understanding the molecular mechanisms that define cancer cell migration.
authors.affiliation: Division of Toxicology, Leiden/Amsterdam Center for Drug Research, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.
authors.forename: Sylvia E
authors.initials: SE
authors.lastname: Le Dévédec
authors.forename: Kuan
authors.initials: K
authors.lastname: Yan
authors.forename: Hans
authors.initials: H
authors.lastname: de Bont
authors.forename: Veerander
authors.initials: V
authors.lastname: Ghotra
authors.forename: Hoa
authors.initials: H
authors.lastname: Truong
authors.forename: Erik H
authors.initials: EH
authors.lastname: Danen
authors.forename: Fons
authors.initials: F
authors.lastname: Verbeek
authors.forename: Bob
authors.initials: B
authors.lastname: van de Water
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D022082:Cellular Structures / Q000473:pathology; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008853:Microscopy; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D009423:Nervous System Neoplasms / Q000378:metabolism; D015398:Signal Transduction; D014945:Wound Healing