Population data and sequence analysis of a 'new' microsatellite locus HumHUU (D16S3433).
This study reports sequence characteristics and population genetic data on a 'new' STR locus HumHUU (D16S3433) located in the non-coding region of chromosome 16q. Based on a population sample of 306 non-related Polish individuals 205 genotypes and 15 alleles with length range of 157-211bp were distinguished. No deviation from HWE was observed. The sequence analysis of each D16S3433 allele revealed a tetranucleotide repeat motif with a basic sequence structure (AAAA)(0-1)(AAAG)(11-22)(AAAAG)(AAAA)(AG)(AAAAAAG). The power of discrimination is 0.9538, showing a high degree of polymorphism. The presented results demonstrate that the D16S3433 is a useful genetic marker for forensic purposes and paternity testing.
Konarzewska
Magdalena
M
Department of Medical Genetics, Warsaw Medical University, Warsaw, Poland.
Pepinski
Witold
W
Jagiello
A
A
Spolnicka
M
M
Soltyszewski
I
I
Plucienniczak
A
A
Berent
J
J
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011786:Quality Control
D017931:DNA Primers