title: Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
abstract: Poly-gamma-glutamate (gamma-PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the gamma-PGA synthesis ywsC-ywtAB genes in its chromosome, cannot produce gamma-PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in gamma-PGA-producing B. subtilis mutant strains. Mutant B. subtilis PGA6-2 stably produces high levels of gamma-PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6-2 is not only a gamma-PGA producer, but it is also a candidate for the genetic and metabolic engineering of gamma-PGA production.
authors.affiliation: Dept. of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, R.O. China. cmyeh@nchu.edu.tw
authors.forename: Chuan-Mei
authors.initials: CM
authors.lastname: Yeh
authors.forename: Jyh-Perng
authors.initials: JP
authors.lastname: Wang
authors.forename: Shih-Ching
authors.initials: SC
authors.lastname: Lo
authors.forename: Wen-Chia
authors.initials: WC
authors.lastname: Chan
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Lin
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D018698:Glutamic Acid / Q000494:pharmacology; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: C511775:poly(gamma-glutamic acid); D000643:Ammonium Chloride; D011099:Polyglutamic Acid; D018698:Glutamic Acid