[Evaluation of biofilm formation by Staphylococcus aureus isolated from sputum of cystic fibrosis patients].
The purpose of this study was to evaluate two screening methods for detection of biofilm formation by eighty clinical Staphylococcus aureus isolates from patients with cystic fibrosis, and evaluation of biofilm production on the polystyrene 96-well tissue culture plates, depending on media applied. All clinical strains were incubated in three different media: Luria-Bertani broth (LB), tryptic soy broth supplemented with 2% glucose (TSBglu) and brain heart infusion (BHI). Biofilm production was screened by staining with crystal violet (CV) or with 2,3,5-triphenyltetrazolium chloride (TTC). Both CV and TTC assays showed, that all analyzed isolates created biofilm, in all tested media, however with different intensity. In conclusion, the CV method was found to be more sensitive than the TTC method, when we need information about whole mass of biofilm. The most optimal medium for the biofilm culture was LB medium.
Pietruczuk-Padzik
Anna
A
Zakład Mikrobiologii Farmaceutycznej, Warszawski Uniwersytet Medyczny. anna.pietruczuk@gmail.com
Stefańska
Joanna
J
Semczuk
Katarzyna
K
Dzierzanowska
Danuta
D
Tyski
Stefan
S
D001431:Bacteriological Techniques / Q000379:methods; D018441:Biofilms; D003470:Culture Media / Q000145:classification*; D003550:Cystic Fibrosis / Q000150:complications*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000502:physiology*
D003470:Culture Media