Preparation and application of a molecularly imprinted polymer for the determination of trace metolcarb in food matrices by high performance liquid chromatography.
In this article, for the first time, a molecularly imprinted polymer (MIP) for the metolcarb was prepared by bulk polymerization using metolcarb as the template, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The prepared polymer was characterized by FT-IR, static and kinetic adsorption experiments, and the results showed that it has been successfully synthesized and had good selective ability for metolcarb. The MIP was applied as a sorbent in molecularly imprinted SPE coupled with HPLC-UV for separation and determination of trace metolcarb in three kinds of food matrices at three concentration levels. Under the optimal conditions, the LODs (S/N=3) of cabbage, cucumber and pear were 7.622, 6.455 and 13.52 microg/kg, respectively, and recoveries were in the range of 68.80-101.31% with RSD (n=3) below 3.78% in all cases. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available C(18) SPE was performed. The results indicated that molecularly imprinted SPE showed better chromatography, better selectivity and higher recoveries for metolcarb than commercially available C(18) SPE.
Qian
Kun
K
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, Tianjin, P. R. China.
Fang
Guozhen
G
He
Jinxing
J
Pan
Mingfei
M
Wang
Shuo
S
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D048448:Phenylcarbamates / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D048448:Phenylcarbamates; D011108:Polymers; C532346:metolcarb