title: Bacillus subtilis SSE4 produces subtulene A, a new lipopeptide antibiotic possessing an unusual C15 unsaturated beta-amino acid.
abstract: Subtulene A, a new cyclic lipopeptide, was isolated from the culture broth of Bacillus subtilis SSE4. This antibiotic compound contained the seven common alpha-amino acids, L-Asn-1, D-Tyr-2, D-Asn-3, L-Gln-4, L-Pro-5, D-Asn-6, L-Ser-7 and the unique beta-amino acid-8 present in the iturin family. 1D and 2D NMR, as well as MS analyses, identified the beta-amino acid as 3-amino-13-methyltetradec-8-enoic acid, an Iso C15 long chain beta-amino acid. B. subtilis SSE4 was also found to produce iturin A. B. subtilis SSE4 culture filtrate exhibited both antifungal and antibacterial activities.
authors.affiliation: Chemical Biology Program, Chulabhorn Graduate Institute, Bangkok, Thailand.
authors.forename: Nopporn
authors.initials: N
authors.lastname: Thasana
authors.forename: Benjaphorn
authors.initials: B
authors.lastname: Prapagdee
authors.forename: Nuchanart
authors.initials: N
authors.lastname: Rangkadilok
authors.forename: Ratiboot
authors.initials: R
authors.lastname: Sallabhan
authors.forename: Seaim Lwin
authors.initials: SL
authors.lastname: Aye
authors.forename: Somsak
authors.initials: S
authors.lastname: Ruchirawat
authors.forename: Suvit
authors.initials: S
authors.lastname: Loprasert
mesh_terms: D000272:Adipates; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects; D008055:Lipids; D055666:Lipopeptides; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic; D012694:Serine / Q000031:analogs & derivatives; D014443:Tyrosine / Q000378:metabolism
chemical_list: D000272:Adipates; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D008055:Lipids; D055666:Lipopeptides; D010456:Peptides, Cyclic; C069989:serine containing aminolipid; D014443:Tyrosine; D012694:Serine; C013579:iturin A; D001216:Asparagine; C029900:adipic acid