Suspended nanoparticle crystal (S-NPC): A nanofluidics-based, electrical read-out biosensor.
Here we report an approach utilizing a suspended nanoparticle crystal (S-NPC) as an electrical read-out biosensor based on a nanofluidic electrokinetics principle. As a preliminary demonstration, streptavidin-modified S-NPC with a particle diameter of 520 nm was used to detect biotin in a PBS buffer. The present result indicated that the detection range of biotin by this nanofluidics-based biosensor was about 1 nM-10 muM (in 10(-4)x PBS) with a sensitivity of 160 nS/nM. Being easy to get established, low-cost, and having large electrical read-out signal, the present S-NPC is believed to be a promising biosensing scheme in the micro total analysis system.
Lei
Yinhua
Y
Institute of Microelectronics, Peking University, Beijing, PR China.
Xie
Fei
F
Wang
Wei
W
Wu
Wengang
W
Li
Zhihong
Z
D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D004560:Electricity; D044085:Microfluidics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013535:Suspensions
D013535:Suspensions; D001710:Biotin; D019809:Streptavidin