title: Technologies for MHC class I immunoproteomics.
abstract: T cell epitopes are peptides, for instance derived from foreign, mutated or overexpressed proteins, that are displayed by MHC molecules on the cell surface and that are recognized by T lymphocytes. Knowledge of the identity of epitopes displayed by MHC molecules is of high value for diagnostic purposes and for the development of prophylactic and therapeutic immunotherapy regimens. Here we review key techniques in MHC class I epitope definition and we discuss recent developments in epitope discovery and their implications. Developments in epitope discovery strategies should ultimately lead to the definition of the MHC-associated peptidome.
authors.affiliation: Division of Cell Biology, Netherlands Cancer Institute and Netherlands Proteomics Centre, The Netherlands.
authors.forename: Rieuwert
authors.initials: R
authors.lastname: Hoppes
authors.forename: Reggy
authors.initials: R
authors.lastname: Ekkebus
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
authors.forename: Huib
authors.initials: H
authors.lastname: Ovaa
mesh_terms: D000465:Algorithms; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005805:Genes, MHC Class I; D006801:Humans; D007167:Immunotherapy; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte