Drawing the line between commensal and pathogenic Gardnerella vaginalis through genome analysis and virulence studies.
BACKGROUND
Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder. It is associated with risk for preterm birth and HIV infection. The etiology of the condition has been debated for nearly half a century and the lack of knowledge about its cause and progression has stymied efforts to improve therapy and prevention. Gardnerella vaginalis was originally identified as the causative agent, but subsequent findings that it is commonly isolated from seemingly healthy women cast doubt on this claim. Recent studies shedding light on the virulence properties of G. vaginalis, however, have drawn the species back into the spotlight.


RESULTS
In this study, we sequenced the genomes of a strain of G. vaginalis from a healthy woman, and one from a woman with bacterial vaginosis. Comparative analysis of the genomes revealed significant divergence and in vitro studies indicated disparities in the virulence potential of the two strains. The commensal isolate exhibited reduced cytotoxicity and yet the cytolysin proteins encoded by the two strains were nearly identical, differing at a single amino acid, and were transcribed at similar levels. The BV-associated strain encoded a different variant of a biofilm associated protein gene and demonstrated greater adherence, aggregation, and biofilm formation. Using filters with different pore sizes, we found that direct contact between the bacteria and epithelial cells is required for cytotoxicity.


CONCLUSIONS
The results indicated that contact is required for cytotoxicity and suggested that reduced cytotoxicity in the commensal isolate could be due to impaired adherence. This study outlines two distinct genotypic variants of G. vaginalis, one apparently commensal and one pathogenic, and presents evidence for disparate virulence potentials.
Harwich
Michael D
MD
Department of Microbiology and Immunology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, VA 23298, USA.
Alves
Joao M
JM
Buck
Gregory A
GA
Strauss
Jerome F
JF
Patterson
Jennifer L
JL
Oki
Aminat T
AT
Girerd
Philippe H
PH
Jefferson
Kimberly K
KK
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D002584:Cervix Uteri / Q000166:cytology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology*; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D037521:Virulence Factors / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D037521:Virulence Factors