PARP activity and NAD concentration in PMC from patients affected by systemic sclerosis and lupus erythematosus.
The enzyme poly(ADP-ribose) polymerase (PARP-1, EC 2.4.2.30) is activated by DNA strand breaks caused by several agents and utilizes NAD to form polyADPR, bound to acceptor proteins. The involvement of PARP-1 in autoimmune diseases has been suggested: antiPARP autoantibodies are described in systemic lupus erythematosus (SLE), DNA strand breaks have been evidenced in systemic sclerosis (SSc). We tested poly(ADP-ribosyl)ation activity and NAD concentration in PMC from patients affected by SLE or SSc and from controls. Lower PARP-1 activity and higher NAD concentration were observed in pathological conditions than controls, supporting the role of PARP-1 activation in modulating NAD concentration.
Cerboni
B
B
Department of Molecular Biology, Section of Biological Chemistry, University of Siena, Siena, Italy. cerboni@unisi.it
Di Stefano
A
A
Micheli
V
V
Morozzi
G
G
Pompucci
G
G
Sestini
S
S
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology* / Q000378:metabolism; D008875:Middle Aged; D009243:NAD / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000201:enzymology* / Q000378:metabolism; D055815:Young Adult
D009243:NAD; D011065:Poly(ADP-ribose) Polymerases