Clinical significance of human kallikrein7 gene expression in colorectal cancer.
BACKGROUND
The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.


METHODS
KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.


RESULTS
Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.


CONCLUSIONS
We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.
Inoue
Yasuhiro
Y
Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Mie, Japan. yasinoue@clin.medic.mie-u.ac.jp
Yokobori
Takehiko
T
Yokoe
Takeshi
T
Toiyama
Yuji
Y
Miki
Chikao
C
Mimori
Koshi
K
Mori
Masaki
M
Kusunoki
Masato
M
D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D014408:Biomarkers, Tumor; C082472:KLK7 protein, human; D007610:Kallikreins