The influence of Ceratocystis polonica inoculation and methyl jasmonate application on terpene chemistry of Norway spruce, Picea abies.
Constitutive and inducible terpene production is involved in conifer resistance against bark beetles and their associated fungi. In this study 72 Norway spruce (Picea abies) were randomly assigned to methyl jasmonate (MJ) application, inoculation with the bluestain fungus Ceratocystis polonica, or no-treatment control. We investigated terpene levels in the stem bark of the trees before treatment, 30 days and one year after treatment using GC-MS and two-dimensional GC (2D-GC) with a chiral column, and monitored landing and attack rates of the spruce bark beetle, Ips typographus, on the trees by sticky traps and visual inspection. Thirty days after fungal inoculation the absolute amount and relative proportion of (+)-3-carene, sabinene, and terpinolene increased and (+)-alpha-pinene decreased. Spraying the stems with MJ tended to generally increase the concentration of most major terpenes with minor alteration to their relative proportions, but significant increases were only observed for (-)-beta-pinene and (-)-limonene. Fungal inoculation significantly increased the enantiomeric ratio of (-)-alpha-pinene and (-)-limonene 1 month after treatment, whereas MJ only increased that of (-)-limonene. One year after treatment, both MJ and fungal inoculation increased the concentration of most terpenes relative to undisturbed control trees, with significant changes in (-)-beta-pinene, (-)-beta-phellandrene and some other compounds. Terpene levels did not change in untreated stem sections after treatment, and chemical induction by MJ and C. polonica thus seemed to be restricted to the treated stem section. The enantiomeric ratio of (-)-alpha-pinene was significantly higher and the relative proportions of (-)-limonene were significantly lower in trees that were attractive to bark beetles compared to unattractive trees. One month after fungal inoculation, the total amount of diterpenes was significantly higher in putative resistant trees with shorter lesion lengths than in putative susceptible trees with longer lesions. Thus, terpene composition in the stem bark may be related to resistance of Norway spruce against I. typographus and C. polonica.
Zhao
Tao
T
Ecological Chemistry Group, Department of Chemistry, Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
Krokene
Paal
P
Björklund
Niklas
N
Långström
Bo
B
Solheim
Halvor
H
Christiansen
Erik
E
Borg-Karlson
Anna-Karin
AK
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D001203:Ascomycota / Q000502:physiology*; D000081008:Bicyclic Monoterpenes; D001517:Coleoptera / Q000382:microbiology*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000032:analysis; D003517:Cyclopentanes / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009664:Norway; D054883:Oxylipins / Q000494:pharmacology*; D028222:Picea / Q000382:microbiology*; D024301:Plant Bark / Q000737:chemistry; D010935:Plant Diseases / Q000382:microbiology*; D018547:Plant Stems / Q000737:chemistry; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis / Q000378:metabolism*
D000085:Acetates; D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D003517:Cyclopentanes; D039821:Monoterpenes; D054883:Oxylipins; D013729:Terpenes; C058582:beta-phellandrene; C035127:sabinene; C072239:methyl jasmonate; C030218:3-carene