Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.
Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis.
Cascioferro
Alessandro
A
Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35121 Padova, Italy.
Boldrin
Francesca
F
Serafini
Agnese
A
Provvedi
Roberta
R
Palù
Giorgio
G
Manganelli
Riccardo
R
D005827:Genetics, Microbial / Q000379:methods*; D016254:Mutagenesis, Insertional / Q000379:methods*; D020102:Mycobacterium smegmatis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011995:Recombination, Genetic; D017384:Sequence Deletion