title: Comparative study of the clastogenicity of functionalized and nonfunctionalized multiwalled carbon nanotubes in bone marrow cells of Swiss-Webster mice.
abstract: The development of nanotechnologies may lead to environmental release of nanomaterials that are potentially harmful to human health. Among the nanomaterials, multiwalled carbon nanotubes (MWCNTs) are already commercialized in various products which can be in direct contact with populations. However, few studies address their potential toxicity. Although a few reports on the cytotoxicity of carbon nanotubes (CNTs) have been published, very little is known about their toxicity or genotoxicity in mammalian cells. We have for the first time compared the clastogenic/genotoxic potential of functionalized and nonfunctionalized MWCNTs in bone marrow cells of Swiss-Webster mice; using mitotic index (MI), chromosome aberrations (CA), micronuclei (MN) formation, and DNA damage in leukocytes as toxicologic endpoints. Six groups of five male mice, each weighing ∼30 ± 2 g, were administered intraperitoneally, once a day for five days with doses of 0.25, 0.5, 0.75, mg/kg body weight (BW) of functionalized and nonfunctionalized MWCNTs. Four vehicle control groups (negative) and a positive control group (carbon black) were also made of 5 mice each. Chromosome and micronuclei from bone marrow cells and comet slides from leukocytes were examined following standard protocols. The results demonstrated that MWCNTs exposure significantly increased (P < 0.05) the number of structural chromosomal aberrations, the frequency of micronucleated cells and the level of DNA damage, and decreased the mitotic index in treated groups compared to control groups. MWCNTs were shown to be toxic at sufficiently high concentrations, however purified functionalized MWCNTs had a higher clastogenic/genotoxic potential compared to nonfunctionalized form of MWCNT. The results of our study suggest that exposure to MWCNT has the potential to cause genetic damage. Hence, careful monitoring should be done with respect to designing/synthesizing biocompatible carbon nanomaterials. Further characterization of their systemic toxicity, genotoxicity and carcinogenicity is also essential.
authors.affiliation: Molecular Toxicology Research Laboratory, NIH-RCMI-Center for Environmental Health, Jackson State University, 1400 Lynch Street, Box 18540, Jackson, Mississippi 39217, USA. anita.k.patlolla@jsums.edu
authors.forename: Anita K
authors.initials: AK
authors.lastname: Patlolla
authors.forename: Saber M
authors.initials: SM
authors.lastname: Hussain
authors.forename: John J
authors.initials: JJ
authors.lastname: Schlager
authors.forename: Srikant
authors.initials: S
authors.lastname: Patlolla
authors.forename: Paul B
authors.initials: PB
authors.lastname: Tchounwou
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000633:toxicity*; D017382:Reactive Oxygen Species
chemical_list: D001672:Biocompatible Materials; D009153:Mutagens; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species