[Development and evaluation of whole-genome DNA microarray of Vibrio parahaemolyticus].
OBJECTIVE
We developed and evaluated a whole-genome DNA microarray of Vibrio parahaemolyticus.


METHODS
Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results.


RESULTS
Microarray hybridization results were completely consistent with theory expectations and PCR verification results.


CONCLUSIONS
We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.
Han
Haihong
H
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. shanlushanlu@163.com
Zeng
Xiaotao
X
Yin
Shengjun
S
Yang
Ruifu
R
Liu
Xiumei
X
Zhou
Dongsheng
D
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000502:physiology
D004269:DNA, Bacterial