title: Taenia saginata: production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens.
abstract: Cysticercosis is a major cause of economic loss in bovine production due to meat condemnation. Chemotherapy is being used in Brazilian cattle and a diagnostic test to improve the treatment program is desired. We produced monoclonal antibodies against crude (TAEB) and cyst fluid (TAEF) Taenia saginata metacestode antigens using immunized BALB/c mice. After cell fusion, 10 TAEB and nine TAEF hybrids were selected and cloned resulting in 18 IgG(1) and 32 IgM TAEB clones, and 9 IgG(1) and 9 IgM TAEF clones. Ascites was produced and Western blot testing was performed resulting in reactivity to protein fractions of low molecular weight (<18kDa), 43, 55, 66 and 100kDa. The indirect immunofluorescence test, with one monoclonal antibody against crude and one against cyst fluid antigens, recognized antigenic fractions of both the scolex and the bladder wall of metacestodes from naturally infected bovine.
authors.affiliation: UNESP, Univ. Estadual Paulista, Campus Botucatu, Graduation Program on Veterinary Medicine, School of Veterinary Medicine and Zootechny, Botucatu, SP, Brazil.
authors.forename: Josy Campanhã
authors.initials: JC
authors.lastname: Vicentini-Oliveira
authors.forename: Marjorie A
authors.initials: MA
authors.lastname: Golim
authors.forename: Silvana
authors.initials: S
authors.lastname: de Cássia Paulan
authors.forename: Germano Francisco
authors.initials: GF
authors.lastname: Biondi
authors.forename: Rosana
authors.initials: R
authors.lastname: Rossi-Ferreira
authors.forename: Elenice
authors.initials: E
authors.lastname: Deffune
authors.forename: Cáris Maroni
authors.initials: CM
authors.lastname: Nunes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D041202:Taenia saginata / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth