title: Detection of adulteration of anti-hypertension dietary supplements and traditional Chinese medicines with synthetic drugs using LC/MS.
abstract: A sensitive and specific liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) method was developed for the analysis of 18 drugs used for the treatment of anti-hypertension, including diuretics, calcium antagonists, and angiogenesis-converting enzyme inhibitors (ACEI) as adulterants in dietary supplements and traditional Chinese medicines. Separation was accomplished on a Xtimate C18 reversed-phase column using a mixture of methanol, acetonitrile and 20 mM ammonium formate buffer (pH 3.2) as mobile phase. The method demonstrated linearity from 0.03 to 21.52 mg kg(-1). Limits of detection ranged from 6.5 to 86.0 microg kg(-1). The recoveries of spiked samples ranged from 71% to 109%. The procedure was successfully applied in routine inspection analysis.
authors.affiliation: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Lu
authors.forename: N L
authors.initials: NL
authors.lastname: Zhou
authors.forename: S Y
authors.initials: SY
authors.lastname: Liao
authors.forename: N
authors.initials: N
authors.lastname: Su
authors.forename: D X
authors.initials: DX
authors.lastname: He
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Tian
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: S Z
authors.initials: SZ
authors.lastname: Yao
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry; D000959:Antihypertensive Agents / Q000032:analysis* / Q000737:chemistry*; D002121:Calcium Channel Blockers / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase; D019587:Dietary Supplements / Q000032:analysis*; D004232:Diuretics / Q000032:analysis / Q000737:chemistry; D004340:Drug Contamination; D004335:Drug and Narcotic Control / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005506:Food Contamination; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D004232:Diuretics; D004365:Drugs, Chinese Herbal