[Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis].
OBJECTIVE
To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.


METHODS
The content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.


RESULTS
Active ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).


CONCLUSIONS
Active ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.
Chen
Shunqin
S
Wuhan Polytechnic University, Wuhan 430023, China.
Yuan
Yuan
Y
Luo
Yujiang
Y
Huang
Luqi
L
Chen
Ping
P
Li
Xiaoming
X
D000975:Antioxidants / Q000494:pharmacology; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D044950:Flavanones / Q000032:analysis* / Q000235:genetics / Q000528:radiation effects; D005419:Flavonoids / Q000032:analysis / Q000235:genetics / Q000494:pharmacology / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D008027:Light; D008516:Medicine, Chinese Traditional / Q000379:methods; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry / Q000254:growth & development*; D013535:Suspensions / Q000737:chemistry
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D013535:Suspensions; C038044:baicalin; C006680:baicalein