Modulation of miR-122 on persistently Borna disease virus infected human oligodendroglial cells.
Using RNAhybrid software we found the predicted binding of complementary sequences between miR-122 and viral mRNAs, may be important for the antiviral effect of miR-122 on Borna disease virus (BDV). A moderate expression of miR-122 was identified in human oligodendroglial cells (OL), but with a much lower level of miR-122 in BDV persistent infection (OL/BDV) and cells transfected with BDV gene expression vectors. Over-expression of miR-122 and specific blocking experiments demonstrated that miR-122 was able to specifically inhibit BDV protein synthesis, viral gene replication and transcription, and induce the secretion/synthesis of interferon (IFN) in OL and OL/BDV cells. The abolishment of miR-122 by AMO-122 inhibited endogenous IFN induction by IFN-beta. These results indicate that miR-122 can exert direct antiviral function by inhibiting BDV translation and replication on one hand, while acting indirectly through IFN to increase the host innate immunity to modulate the virus-host interactions on the other hand.
Qian
Jun
J
Department of Microbiology, Harbin Medical University; Immunity and Infection, Pathogenic Biology Key Laboratory, Heilongjiang Province, Harbin 150086, China.
Zhai
Aixia
A
Kao
Wenping
W
Li
Yujun
Y
Song
Wuqi
W
Fu
Yingmei
Y
Chen
Xiaobei
X
Zhang
Qingmeng
Q
Wu
Jing
J
Li
Hui
H
Zhong
Zhaohua
Z
Ling
Hong
H
Zhang
Fengmin
F
D001891:Borna disease virus / Q000276:immunology* / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D006801:Humans; D007372:Interferons / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D009836:Oligodendroglia / Q000276:immunology* / Q000821:virology*; D014764:Viral Proteins / Q000096:biosynthesis
C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D014764:Viral Proteins; D007372:Interferons