title: Identification of immunologically relevant proteins of Chlamydophila abortus using sera from experimentally infected pregnant ewes.
abstract: Chlamydophila abortus is an intracellular pathogen and the etiological agent of enzootic abortion of ewes (EAE). C. abortus has a biphasic development cycle; extracellular infectious elementary bodies (EB) attach and penetrate host cells, where they give rise to intracellular, metabolically active reticulate bodies (RB). RB divide by binary fission and subsequently mature to EB, which, on rupture of infected cells, are released to infect new host cells. Pregnant ewes were challenged with 2 x 10(6) inclusion forming units (IFU) of C. abortus cultured in yolk sac (comprising both EB and RB). Serum samples were collected at 0, 7, 14, 21, 27, 30, 35, 40, and 43 days postinfection (dpi) and used to identify antigens of C. abortus expressed during disease. Additionally, sera from fetal lambs were collected at 30, 35, 40, and 43 dpi. All serum samples collected from experimentally infected pregnant ewes reacted specifically with several antigens of EB as determined by one-dimensional (1-D) and 2-D gel electrophoresis; reactive antigens identified by mass spectrometry included the major outer membrane protein (MOMP), polymorphic outer membrane protein (POMP), and macrophage infectivity potentiator (MIP) lipoprotein.
authors.affiliation: UCD School of Agriculture Food Science & Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: P X
authors.initials: PX
authors.lastname: Marques
authors.forename: Puneet
authors.initials: P
authors.lastname: Souda
authors.forename: J
authors.initials: J
authors.lastname: O'Donovan
authors.forename: J
authors.initials: J
authors.lastname: Gutierrez
authors.forename: E J
authors.initials: EJ
authors.lastname: Gutierrez
authors.forename: S
authors.initials: S
authors.lastname: Worrall
authors.forename: M
authors.initials: M
authors.lastname: McElroy
authors.forename: A
authors.initials: A
authors.lastname: Proctor
authors.forename: C
authors.initials: C
authors.lastname: Brady
authors.forename: D
authors.initials: D
authors.lastname: Sammin
authors.forename: H F
authors.initials: HF
authors.lastname: Basset
authors.forename: Julian P
authors.initials: JP
authors.lastname: Whitelegge
authors.forename: B E
authors.initials: BE
authors.lastname: Markey
authors.forename: J E
authors.initials: JE
authors.lastname: Nally
mesh_terms: D037881:Aborted Fetus / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western; D023501:Chlamydophila / Q000276:immunology*; D023521:Chlamydophila Infections / Q000276:immunology / Q000662:veterinary; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D013058:Mass Spectrometry; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins