An approach towards peptide-based antibody detection for diagnosis of Chikungunya infection.
Chikungunya infection, although rarely fatal, is associated with significant morbidity which necessitates its diagnosis in the initial stages. Currently for diagnosis, together with clinical symptoms, immunological methods such as IgG/IgM detection, molecular methods such as real-time reverse transcriptase-polymerase chain reaction and viral isolation methods are available but they are either not very specific or they require high-level sophisticated infrastructures. In the present study, an enzyme-linked immunosorbent assay to evaluate antibody responses to peptides designed from the CHIKV E2 envelope glycoprotein was performed. Synthesized peptides were evaluated with confirmed Chikungunya and non-Chikungunya serum samples for antibody detection. The results demonstrate that the synthetic peptide-based diagnosis of Chikungunya can be an efficient and a more accessible approach in immunodiagnostics.
Morey
Shweta H
SH
Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Bajaj Nagar, Nagpur, India.
Kashyap
Rajpal S
RS
Purohit
Hemant J
HJ
Taori
Girdhar M
GM
Daginawala
Hatim F
HF
D018354:Alphavirus Infections / Q000175:diagnosis* / Q000276:immunology*; D000595:Amino Acid Sequence; D000906:Antibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002646:Chikungunya virus; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007159:Immunologic Tests; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology* / Q000302:isolation & purification; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000032:analysis*
D000906:Antibodies; D015415:Biomarkers; D010455:Peptides; D014764:Viral Proteins