Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range.
A rapid positive screening system for identifying recombinant baculovirus with an expanded host range was developed by using an actin promoter-dependent fluorescent protein gene expression cassette. The expression of recombinant protein regulated by the polyhedrin promoter was not affected by the insertion of the cassette in the baculovirus genome.
Watanabe
Satoko
S
Research Team for Advanced Biologicals, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. satochan@affrc.go.jp
Ohta
Masato
M
Kokuho
Takehiro
T
Mori
Hajime
H
Inumaru
Shigeki
S
D016367:Baculoviridae / Q000235:genetics* / Q000302:isolation & purification*; D015967:Gene Expression Regulation, Viral; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000235:genetics; D014773:Virology / Q000379:methods*
D011994:Recombinant Proteins