[Research of cerebral mitochondrial dysfunction in septic rats].
OBJECTIVE
To study the pathogenesis of cerebral mitochondrial dysfunction in septi rats.


METHODS
Forty clean level SD male rats were randomly divided into 4 groups: control group, 3-sepsis group, 6 h sepsis group and 24 h sepsis group. The sepsis model was established by intraperitoneal injection of lipopolysaccharide (LPS). The membrane potential of cerebral mitochondrion was detected by flow cytometry. Mitochondrion of four kinds of nerve cells were observed by electronic microscopy and the degree of injury was analyzed by Flameng semiquantitative evaluation of ultrastructure.


RESULTS
Cerebral mitochondrial membrane potential in 3-sepsis group decreased significantly compared with that in control group [(0.05 +/- 0.05) vs (1.20 +/- 0.6)], but the brain mitochondrial membrane potential in 6-hour and 24 h LPS group were not significant difference as compared with control group [(1.4 +/- 0.7) vs (1.2 +/- 0.6), (1.6 +/- 0.7) vs (1.2 +/- 0.6)]. Mitochondrial ultrastructure injury evaluation score of neuronal and astroglial in 6 h LPS group rats were increased significantly as compared with control group, but the score of microglial and oligodendroglial in all the 4 groups didn not have significant difference.


CONCLUSIONS
The injury of rats brain mitochondria was reversible in early sepsis and mitochondrial dysfunction occurred more earlier than the ultrastructure change. Compared with neuronal and astroglial, mitochondrial of microglial and oligodendroglial were more sensitive to septic injury.
Li
Ning
N
Department of Neonate, Nanfang Hospital, Southern Medical University, Guangzhou, China. ningli@fimmu.com
Wang
Yang
Y
Zen
Qi-yi
QY
D000818:Animals; D054022:Cerebrum / Q000473:pathology*; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D009457:Neuroglia / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000473:pathology*