title: High-intensity interval training increases SIRT1 activity in human skeletal muscle.
abstract: The effects of training on silent mating-type information regulator 2 homolog 1 (SIRT1) activity and protein in relationship to peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) and mitochondrial content were determined in human skeletal muscle. Six weeks of high-intensity interval training ( approximately 1 h of 10 x 4 min intervals at 90% peak oxygen consumption separated by 2 min rest, 3 days per week) increased maximal activities of mitochondrial enzymes in skeletal muscle by 28% to 36% (citrate synthase, beta-hydroxyacyl-coenzyme A dehydrogenase, and cytochrome c oxidase subunit IV) and PGC-1alpha protein (16%) when measured 4 days after training. Interestingly, total muscle SIRT1 activity (31%) and activity per SIRT1 protein (58%) increased despite decreased SIRT1 protein (20%). The present data demonstrate that exercise-induced mitochondrial biogenesis is accompanied by elevated SIRT1 activity in human skeletal muscle.
authors.affiliation: School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6, Canada.
authors.forename: Brendon J
authors.initials: BJ
authors.lastname: Gurd
authors.forename: Christopher G R
authors.initials: CG
authors.lastname: Perry
authors.forename: George J F
authors.initials: GJ
authors.lastname: Heigenhauser
authors.forename: Lawrence L
authors.initials: LL
authors.lastname: Spriet
authors.forename: Arend
authors.initials: A
authors.lastname: Bonen
mesh_terms: D001642:Bicycling; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000378:metabolism; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000201:enzymology*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D005082:Physical Exertion; D056564:Sirtuin 1 / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D024101:Mitochondrial Proteins; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C499631:TFAM protein, human; D014157:Transcription Factors; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D004746:Enoyl-CoA Hydratase