Enhanced expression of glutathione S-transferase in the hippocampus following acute treatment with trimethyltin in vivo.
We investigated the effects of treatment with trimethyltin (TMT) on the expression of glutathione-related enzymes in mouse hippocampus. TMT promoted the expression of glutathione S-transferase (GST) Ya/Yc mRNA, and GSTA2 protein, but not that of glutamate-cysteine ligase catalytic subunit mRNA, 1 day after injection. TMT produced a slight but significant elevation of GST activity during the period from day 1 to 7 post-treatment. No significant change was seen in the activity of glutathione peroxidase at anytime post-TMT treatment. Our data suggest the prolonged elevation of GST activity in the hippocampus following TMT treatment through enhanced expression of the GST Ya/Yc.
Nagashima
Reiko
R
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.
Sano
Shohei
S
Huong
Nguyen Quynh
NQ
Shiba
Tatsuo
T
Ogita
Kiyokazu
K
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D049669:History, 16th Century; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014298:Trimethyltin Compounds / Q000633:toxicity*
D007527:Isoenzymes; D016877:Oxidants; D012333:RNA, Messenger; D014298:Trimethyltin Compounds; C046488:trimethyltin; D005979:Glutathione Peroxidase; C105859:glutathione S-transferase Yc; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione