title: Hansschlegelia zhihuaiae sp. nov., isolated from a polluted farmland soil.
abstract: A novel Gram-negative, aerobic, coccoid-shaped strain designated S 113(T) was isolated from a polluted-soil sample collected in Jiangsu Province, China. A polyphasic taxonomic study including phylogenetic analysis based on the 16S rRNA gene sequence and determination of phenotypic characteristics was performed on the new isolate. The highest 16S rRNA gene sequence similarity was 96.8 %, with Hansschlegelia plantiphila S(1)(T). The predominant respiratory quinone was ubiquinone 10 (Q-10). The major fatty acids were C(18 : 1)ω7c and C(16 : 0). The G+C content of the DNA was about 65.7 mol%. DNA-DNA hybridization experiments showed 44.9 % relatedness for strain S 113(T) with its closest relative, H. plantiphila NCIMB 14035(T). The dominant phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylcholine. The results of our polyphasic taxonomic analysis indicate that strain S 113(T) represents a novel species within the genus Hansschlegelia, for which the name Hansschlegelia zhihuaiae sp. nov. is proposed. The type strain is S 113(T) ( = DSM 18984(T)  = CCTCC AB 206143(T)  = KCTC 12880(T)).
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences (ZAAS), Hangzhou, Zhejiang 310021, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
mesh_terms: D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D042083:Methylocystaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants