The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats.
The aims of this study were to clarify the effects of high dietary supplementation with boric acid and borax, called boron (B) compounds, on lipid peroxidation (LPO), antioxidant activity, some vitamin levels, and DNA damage in rats. Thirty Sprague Dawley male rats were divided into three equal groups: the animals in the first group (control) were fed with a standard rodent diet containing 6.4 mg B/kg, and the animals in the experimental group were fed with a standard rodent diet added with a supra-nutritional amount of boric acid and borax (100 mg B/kg) throughout the experimental period of 28 days. The B compounds decreased malondialdehyde (MDA), DNA damage, the protein carbonyl content (PCO) level in blood, and glutathione (GSH) concentration in the liver, Cu-Zn superoxide dismutase (SOD), and catalase (CAT) activity in the kidney. The B compounds increased GSH concentration in blood and the vitamin C level in plasma. Consequently, our results demonstrate that B supplementation (100 mg/kg) in diet decreases LPO, and enhances the antioxidant defense mechanism and vitamin status. There are no differences in oxidant/antioxidant balance and biochemical parameters except for serum vitamin A and liver GSH concentration, between the boron compounds used in this study.
Ince
Sinan
S
Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyonkarahisar, Turkey. since@aku.edu.tr
Kucukkurt
Ismail
I
Cigerci
Ibrahim Hakki
IH
Fatih Fidan
A
A
Eryavuz
Abdullah
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D001881:Borates / Q000494:pharmacology*; D001888:Boric Acids / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D004249:DNA Damage; D019587:Dietary Supplements; D005978:Glutathione / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood; D050050:Protein Carbonylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D014801:Vitamin A / Q000097:blood; D019207:beta Carotene / Q000097:blood
D000975:Antioxidants; D001881:Borates; D001888:Boric Acids; D019207:beta Carotene; D014801:Vitamin A; C018851:borax; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid; C032688:boric acid