title: Recombinant N-terminal part of bovine herpesvirus 1 ICP27 protein: its preparation, purification, and use for raising specific antiserum.
abstract: Recombinant N-terminal part of bovine herpesvirus-1 (BoHV-1) ICP27 protein fused with thioredoxin and His-tag ("the recombinant protein") expressed in Escherichia coli was purified by the Ni2+-NTA affinity chromatography and used for the preparation of antiserum by immunization of rabbits. The antiserum recognized the recombinant protein in Western blot analysis and was able to detect BoHV-1 ICP27 in the nucleoli of BoHV-1-infected MDBK cells. These results showed that such an antiserum could serve as a valuable tool in further studies of the functions of BoHV-1 ICP27.
  
  
  BACKGROUND
  Bovine herpesvirus 1, ICP27, recombinant protein, antiserum, immunofluorescence assay.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences Wuhan 430071, PR, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Ren
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
authors.forename: A C
authors.initials: AC
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004285:Dogs; D007242:Herpesvirus 1, Bovine / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins