[Molecular and histological detection of Helicobacter pylori and genotyping based on babA2 and iceA in patients with benign gastric pathologies].
OBJECTIVE
To investigate the presence of Helicobacter pylori in a Colombian population.


METHODS
Gastric biopsies from 60 patients with benign gastric pathologies were submitted to histopathological examination and genotyped by PCR through amplification of babA2 and iceA genes.


RESULTS
The presence of H. pylori was demonstrated in 78.3%, 63.3% and 66.7% of the samples after Giemsa staining, andH. pylori 16S DNAr and iceA gene amplification, respectively. In addition, H. pylori babA2 positive was found infecting 7 patients. Among the iceA positive samples, 35% were identified as iceAl, 47.5% as iceA2 and 17.5% contained iceAl and iceA2 or different iceA2 types. This work allowed detection and genotyping of H. pylori, demonstrating a low proportion of patients carrying strains babA2 positive and an iceA genotype distribution according to previous reports. No association was found between the presence of babA2 and iceA genes and ulcer disease.
Montealegre O
M Camila
MC
Universidad de los Andes, Bogotá, Colombia.
Jaramillo H
Carlos Alberto
CA
Montealegre L
Gustavo
G
Parra G
Giovanna
G
Echeverry de P
M Magdalena
MM
Delgado P
M del Pilar
Mdel P
D018829:Adhesins, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001706:Biopsy; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005773:Gastroscopy; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013276:Stomach Ulcer / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors; C063113:ice nucleation protein