Antioxidant activity and hepatoprotective property of leaf extracts of Boerhaavia diffusa Linn against acetaminophen-induced liver damage in rats.
Extracts of Boerhaavia diffusa leaves were evaluated for antioxidant and hepatoprotective properties in the acetaminophen-induced liver damage model. Antioxidative evaluation of ethanolic extract gave total phenolic content, total flavonoid content, vitamin C content and vitamin E content and the levels of selenium and zinc as 6.6+/-0.2mg/g tannic acid equivalent, 0.092+/-0.003 mg/g quercetin equivalent, 0.21+/-0.03 mg/g, 0.054+/-0.002 mg/g, 0.52+/-0.05 ppm and 9.28+/-0.16 ppm, respectively. The DPPH scavenging capacity and the reductive potential were 78.32+/-2.41% and 0.65+/-0.02 mg/g ascorbic acid, respectively. Pretreatment with aqueous and ethanolic extracts decreased the activities of alkaline phosphatase, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, and the level of bilirubin in the serum that were elevated by acetaminophen. The two extracts also ameliorated the elevation in the activities of the enzymes in the liver. Acetaminophen intoxication led to reduction in serum and liver albumin levels which were not significantly increased by pretreatment with the extracts. The extracts also protected against acetaminophen induced lipid peroxidation. These results indicated that leaf extracts from B. diffusa possess hepatoprotective property against acetaminophen-induced liver damage which may be mediated through augmentation of antioxidant defenses.
Olaleye
M Tolulope
MT
Department of Biochemistry, School of Sciences, Federal University of Technology, PMB 704, Akure, Nigeria.
Akinmoladun
Afolabi C
AC
Ogunboye
Adebayo A
AA
Akindahunsi
Afolabi A
AA
D000082:Acetaminophen / Q000037:antagonists & inhibitors / Q000633:toxicity; D018712:Analgesics, Non-Narcotic / Q000037:antagonists & inhibitors / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000032:analysis; D001713:Biphenyl Compounds / Q000737:chemistry; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008111:Liver Function Tests; D000432:Methanol; D039281:Nyctaginaceae / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D012643:Selenium / Q000032:analysis; D012997:Solvents; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000032:analysis; D014867:Water; D015032:Zinc / Q000032:analysis
D018712:Analgesics, Non-Narcotic; D000975:Antioxidants; D001713:Biphenyl Compounds; D016877:Oxidants; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D012997:Solvents; D017392:Thiobarbituric Acid Reactive Substances; D014867:Water; D014810:Vitamin E; D000082:Acetaminophen; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium; D015032:Zinc; D001205:Ascorbic Acid; D000432:Methanol