title: Separation of optical isomers in capillary chromatography using a poly(tetrafluoroethylene) capillary tube and an aqueous-organic mixture carrier solution.
abstract: Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture as a carrier solution. The open tubular capillary was 110 cm in length (90 cm effective length) and 100 microm in inner diameter. The carrier solution was prepared with a water-acetonitrile-ethyl acetate mixture (15:3:2 volume ratio) containing 1 mM beta-cyclodextrin. A model analyte solution of dansyl-DL-methionine was injected into the capillary tube by a gravity method. The analyte solution was subsequently delivered through the capillary tube with the carrier solution by a microsyringe pump; the system worked under laminar-flow conditions. The analytes were separated through the capillary tube with on-capillary detection by an absorption detector. D-Isomer and L-isomer were eluted in this order with the water-acetonitrile-ethyl acetate carrier solution including beta-cyclodextrin.
authors.affiliation: Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Ishimoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Jinno
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tsukagoshi