Avian leukosis virus p27 inhibits tumor necrosis factor alpha expression in RAW264.7 macrophages after stimulation with lipopolysaccharide.
Avian leukosis is a widely distributed disease caused by Avian leukosis virus (ALV). ALV p27 is a capsid protein and group specific antigen, whose role in host immune response is poorly understood. To explore ALV p27, we developed a RAW264.7 macrophage cell line stably expressing p27-GFP fusion protein. The cells of this line and control cell line expressing GFP protein only were compared in production of tumor necrosis factor alpha (TNF-alpha), interleukins IL-1beta, IL-6, IL-12, and in proliferative activity stimulated by the lipopolysaccharide (LPS). It was found that the ALV p27 expression markedly reduced the production of TNF-alpha, but did not affect the production of IL-1beta, IL-6, and 12, and cell proliferative activity. These results demonstrate that ALV p27 specifically inhibits TNF-alpha expression in the macrophages stimulated by LPS, suggesting a possible contribution of ALV p27 to immunosuppression detected in ALV-infected hosts.


BACKGROUND
Avian leukosis virus; p27; tumor necrosis factor alpha; interleukins; macrophages; lipopolysaccharide.
Pang
P
P
State Key Laboratory of Agrobiotechnology, Beijing 100193, PR, China.
Chen
F Y
FY
Tang
J
J
Ma
L F
LF
Wang
J C
JC
Zheng
S J
SJ
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001354:Avian Leukosis Virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation; D004279:DNA, Viral / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000276:immunology; D007108:Immune Tolerance; D018664:Interleukin-12 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000821:virology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D049452:Green Fluorescent Proteins; D018664:Interleukin-12