title: Comparison of cellular response to anorganic bone matrix/cell binding peptide and allogenic cranial bone after sinus augmentation in rhesus monkeys.
abstract: This study compared cellular responses of maxillary sinuses after augmentation with anorganic bovine-derived hydroxyapatite matrix linked to the cell binding polypeptide P-15 (ABM/P-15) or PepGen P-15 and allogenic freeze-dried cranial bone slabs. Five adult Macaque fascicularis monkeys were used. On one side, the floor of the sinus was augmented with ABM/P-15, while the other side was augmented with 2 cranial bone slabs. Trephine bone biopsies were obtained 6, 12, and 24 weeks postgrafting. Animals were sacrificed 8 months after grafting. Soft X-ray microradiography was used to determine bone density. The volume fraction (Vv) of regenerated bone, the number of mesenchymal cells, and the numbers of proliferating cell nuclear antigen (PCNA)- and alkaline phosphatase-positive cells at different augmentation sites were measured and compared. Basal bone heights were calculated at surgery and compared with total heights of the augmented sinus floors 8 months postgrafting. Bone formation, number of mesenchymal cells, PCNA index, and alkaline phosphatase index were significantly higher for the ABM/P-15 side than for the allogenic bone-augmented side. Both sides ended with a significant increase in bone height. The PCNA index decreased significantly over time (P < .05), while the alkaline phosphatase index increased significantly (P < .05) over time on both sides. Both graft materials have led to significant augmentation of the floor of the maxillary sinus with new bone; however, new bone formation and maturation were faster on the ABM/P-15 sites.
authors.affiliation: Oral & Maxillofacial Surgery Department, Cairo University, Cairo, Egypt.
authors.forename: Ihab
authors.initials: I
authors.lastname: El-Madany
authors.forename: Hany
authors.initials: H
authors.lastname: Emam
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sharawy
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000494:pharmacology; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D017886:Durapatite; D008253:Macaca mulatta; D008443:Maxillary Sinus / Q000601:surgery*; D008648:Mesoderm / Q000166:cytology; D008852:Microradiography; D013516:Oral Surgical Procedures, Preprosthetic / Q000379:methods*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis
chemical_list: D018786:Bone Substitutes; C403137:PepGen P-15; D018809:Proliferating Cell Nuclear Antigen; D017886:Durapatite; D000469:Alkaline Phosphatase