title: Effects of myeloperoxidase -463 G/A gene polymorphism and plasma levels on coronary artery disease.
abstract: Myeloperoxidase is a lysosomal enzyme of polymorphonuclear leucocytes that contributes to inflammatory responses. In previous studies it was shown that MPO was synthesized in atherosclerotic lesions responsible of lipoprotein oxidations. We aimed to determine the MPO -463 G/A gene polymorphism distribution in Turkish population and evaluate the effects of it on myeloperoxidase levels. There were 100 myocardial infarct patients and 100 healthy control subjects in our study. MPO polymorphism was studied by using PCR-RFLP technique and MPO levels were measured by ELISA. It was shown that MPO levels were increasing in patients after myocardial infarct event but there were no effect of MPO -463 G/A polymorphism on MPO levels. It was also found that serum total cholesterol and LDL-cholesterol levels and smoking was contributing factors in increments of MPO enzymes. We observed that MPO levels were increased in CAD but there were no effect of MPO -463 G/A polymorphism on MPO levels.
authors.affiliation: Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, 34280, Capa Istanbul, Turkey. a_ergen@yahoo.com
authors.forename: Arzu
authors.initials: A
authors.lastname: Ergen
authors.forename: Selim
authors.initials: S
authors.lastname: İsbir
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Timirci
authors.forename: Atike
authors.initials: A
authors.lastname: Tekeli
authors.forename: Turgay
authors.initials: T
authors.lastname: İsbir
mesh_terms: D000368:Aged; D000483:Alleles; D050197:Atherosclerosis / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000235:genetics; D009195:Peroxidase / Q000097:blood* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012306:Risk; D012907:Smoking / Q000009:adverse effects
chemical_list: D009195:Peroxidase