Umbilical cord blood CD45(+)CD34(+) cells coexpression in preterm and full-term neonates: a pilot study.
OBJECTIVE
Human umbilical cord blood (hUCB) is rich in stem cells. The CD45(+)/CD34(+) coexpression in hUCB is a marker of hemopoietic progenitor cells. The objective of this study is to compare the coexpression of hUCB CD45(+)/CD34(+) cells in preterm (PT) and full-term (FT) neonates.


METHODS
We studied the coexpression of hUCB CD45(+)/CD34(+) cells in PT and FT neonates. The study included 25 PT (29-36 weeks gestation) and 25 FT (37-41) neonates delivered at Ain Shams University, Maternity Hospital. Absolute mononuclear layer cord blood CD45(+)/CD34(+) cell count were measured by flow cytometry. Morbidity was assessed for 12 of the studied 25 PT infants, using Morbidity Assessment Index for Newborns score.


RESULTS
The absolute CD45(+)/CD34(+) count did not differ between PT and FT infants: Z =  -0.485, p = 0.63. There was no correlation between absolute cell count and GA (r = 0.013, p = 0.9) for all 50 neonates. Mode of delivery did not affect the absolute count in the PT infants: Z  =  -0.6, p  = 0.57. There was no correlation between the degree of morbidity and absolute cell count in PT neonates; r = 0.13, p = 0.69.


CONCLUSIONS
The absolute cell count is not affected by gestational age and did not relate to morbidity scores in the studied PT infants. Further, wide-scale work will be needed to study CD45(+)/CD34(+) count in hUCB in sick PT neonates.
Hassanein
Sahar M A
SM
Department of Pediatrics, Ain Shams University School of Medicine, Cairo, Egypt.
Amer
Hanaa A
HA
Shehab
Abeer A
AA
Hellal
Mahmoud M K H
MM
D018952:Antigens, CD34 / Q000378:metabolism*; D001772:Blood Cell Count; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn / Q000097:blood / Q000378:metabolism; D007234:Infant, Premature / Q000097:blood / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D010865:Pilot Projects; D047928:Premature Birth / Q000097:blood / Q000378:metabolism; D047929:Term Birth / Q000097:blood / Q000378:metabolism
D018952:Antigens, CD34; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human