A survey of yeast genomic assays for drug and target discovery.
Over the past decade, the development and application of chemical genomic assays using the model organism Saccharomyces cerevisiae has provided powerful methods to identify the mechanism of action of known drugs and novel small molecules in vivo. These assays identify drug target candidates, genes involved in buffering drug target pathways and also help to define the general cellular response to small molecules. In this review, we examine current yeast chemical genomic assays and summarize the potential applications of each approach.
Smith
Andrew M
AM
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada.
Ammar
Ron
R
Nislow
Corey
C
Giaever
Guri
G
D000935:Antifungal Agents / Q000494:pharmacology; D016503:Drug Delivery Systems; D015195:Drug Design; D020869:Gene Expression Profiling / Q000379:methods; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods*; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics
D000935:Antifungal Agents