title: Imaging and cell targeting characteristics of magnetic nanoparticles modified by a functionalizable zwitterionic polymer with adhesive 3,4-dihydroxyphenyl-l-alanine linkages.
abstract: Multifunctional magnetic nanoparticles (MNPs) modified by a zwitterionic polymer (pCBMA-DOPA(2)) containing one poly(carboxybetaine methacrylate) (pCBMA) chain and two 3,4-dihydroxyphenyl-L-alanine (DOPA) residue groups were developed. Results showed that MNPs modified by pCBMA were not only stable in complex media, but also provided abundant functional groups for ligand immobilization. The pCBMA-DOPA(2) MNPs had a hydrodynamic particle size of about 130 nm, a strong saturation magnetization of 110.2 emu/g Fe and a high transverse relaxivity of 428 mM(-1)s(-1). Long-term stability in phosphate-buffered saline (PBS) and 10% NaCl solution was achieved for over six months. Compared to MNPs coated with dextran, pCBMA-DOPA(2) MNPs presented better stability in 100% human blood serum at 37 degrees C. Macrophage cell uptake studies revealed that the uptake ratio of pCBMA-DOPA(2) MNPs was much lower than that of dextran MNPs. Furthermore, quantitative analysis results showed that after pCBMA-DOPA(2) MNPs were conjugated with a targeting RGD peptide, uptake by human umbilical vein endothelial cell (HUVEC) was notably increased, which was further visualized by magnetic resonance imaging (MRI).
authors.affiliation: Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195, United States.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Xue
authors.forename: Changlu
authors.initials: C
authors.lastname: Gao
authors.forename: Louisa
authors.initials: L
authors.lastname: Carr
authors.forename: Jinnan
authors.initials: J
authors.lastname: Wang
authors.forename: Baocheng
authors.initials: B
authors.lastname: Chu
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000269:Adhesives; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival; D004295:Dihydroxyphenylalanine / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D042783:Endothelial Cells / Q000166:cytology; D005290:Ferric Compounds / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D044967:Serum / Q000378:metabolism
chemical_list: D000269:Adhesives; D005290:Ferric Compounds; D011108:Polymers; C000499:ferric oxide; D004295:Dihydroxyphenylalanine