title: A rapid and most sensitive liquid chromatography/tandem mass spectrometry method for simultaneous determination of alverine and its major metabolite, para hydroxy alverine, in human plasma: application to a pharmacokinetic and bioequivalence study.
abstract: A rapid and highly sensitive method for the determination of alverine (ALV) and its metabolite, para hydroxy alverine (PHA), in human plasma using LC-MS/MS in positive ion electrospray ionization (ESI) in multiple reactions monitoring (MRM) mode was developed and validated. The procedure involves a simple solid phase extraction (SPE). Chromatographic separation was carried out on a Hypersil GOLD C(18) column (50 mm x 4.6 mm, 5 microm) with an isocratic mobile phase and a total run time of 1.5 min. The standard calibration curves showed excellent linearity within the range of 0.060-10.051 ng/mL for ALV and 0.059-10.017 ng/mL for PHA (r > or = 0.990). This method was successfully applied to a pharmacokinetic study after oral administration of alverine citrate 120 mg capsule in Indian healthy male volunteers.
authors.affiliation: Contract Research Organization, Cadila Pharmaceuticals Limited, 1389-Trasad Road, Dholka, Gujarat, India. chinmoy_ghosh@yahoo.com
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Ghosh
authors.forename: Vijay
authors.initials: V
authors.lastname: Jha
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ahir
authors.forename: Sujal
authors.initials: S
authors.lastname: Shah
authors.forename: C P
authors.initials: CP
authors.lastname: Shinde
authors.forename: Bhaswat S
authors.initials: BS
authors.lastname: Chakraborty
mesh_terms: D000284:Administration, Oral; D002214:Capsules; D002853:Chromatography, Liquid / Q000379:methods*; D018592:Cross-Over Studies; D006801:Humans; D008297:Male; D010636:Phenols / Q000097:blood*; D011437:Propylamines / Q000097:blood* / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: C552687:4-hydroxyalverine; D002214:Capsules; D010636:Phenols; D011437:Propylamines; C010846:alverine