[An in vivo study of the biologic activity of the recombinant pseudoadenovirus nanostructure containing lactoferrin gene].
The Ad5-Lf recombinant pseudoadenovirus nanostructure (RPAN) based on adenovirus of the 5th serotype and containing lactoferrin (Lf) gene was constructed. The goal of this work was to develop a system for efficient production of human lactoferrin (Lf) in human body. It was shown using the model of cisplatin (DDP)-induced toxicosis that human Ad5-based RPAN with human Lf gene expressing cassette in its genome provides high rate of expression of Lf gene in animal body. In vivo recombinant human Lf demonstrates detoxification effect against acute DDP-induced toxic reactions similar to that of the native Lf. RPAN does not stimulate growth of primary and metastatic nodes of experimental tumors. Moreover, it inhibits the growth of Lewis lung carcinoma (LLC), Ehrlich carcinoma (ELD), and S37 sarcoma in early periods after tumor transplantation. The obtained experimental data are indicative of the good prospects of further biologic and medical study of RPAN and development of RPAN-based genetic engineering medicine of the new generation.
Iakubovskaia
R I
RI
Bezborodova
O A
OA
Nemtsova
E R
ER
Tutykhina
I L
IL
Shmarov
M M
MM
Logunov
D Iu
DIu
Naroditskii
B S
BS
Chissov
V I
VI
Gintsburg
A L
AL
D000256:Adenoviridae; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000633:toxicity; D005260:Female; D005818:Genetic Engineering; D006801:Humans; D008658:Inactivation, Metabolic; D007781:Lactoferrin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D049329:Nanostructures; D014161:Transduction, Genetic / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D007781:Lactoferrin; D002945:Cisplatin