Whole exome capture in solution with 3 Gbp of data.
We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.
Bainbridge
Matthew N
MN
Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA. bainbrid@bcm.edu
Wang
Min
M
Burgess
Daniel L
DL
Kovar
Christie
C
Rodesch
Matthew J
MJ
D'Ascenzo
Mark
M
Kitzman
Jacob
J
Wu
Yuan-Qing
YQ
Newsham
Irene
I
Richmond
Todd A
TA
Jeddeloh
Jeffrey A
JA
Muzny
Donna
D
Albert
Thomas J
TJ
Gibbs
Richard A
RA
D020029:Base Pairing / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005091:Exons / Q000235:genetics*; D015723:Gene Library; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012996:Solutions
D012996:Solutions