title: Screening and characterization of an inhibitory chemical specific to Arabidopsis gibberellin 2-oxidases.
abstract: The hydroxylation of gibberellin (GA) at the 2-position is known as the major cause of inactivation of GAs, whose reaction is catalyzed by 2-oxoglutarate dependent dioxygenases, also termed GA 2-oxidases (GA2oxs). To block GA catabolism in plants, a few chemicals can be used. To obtain novel inhibitors specific to GA2oxs, we performed in vitro random screenings by using (3)H-16,17-dihydro-GA(4) and recombinant Arabidopsis GA2ox2. As a result, one candidate, methyl 6-chloro-3H-1,2,3-benzodithiazole-4-carboxylate 2-oxide (CBTC), was selected from the screening, and was subjected to in-planta evaluations. CBTC promoted both the germination and elongation of Arabidopsis seedlings. This strongly suggests that CBTC inhibits GA2oxs in Arabidopsis with high specificity.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Otani
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Yoon
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakajima
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D006899:Mixed Function Oxygenases / Q000378:metabolism*
chemical_list: D006899:Mixed Function Oxygenases; C121463:gibberellin 2-dioxygenase