title: Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway.
abstract: Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control.
authors.affiliation: Division of Applied Life Sciences (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
authors.forename: Sang-Hwal
authors.initials: SH
authors.lastname: Yoon
authors.forename: Asad Ali
authors.initials: AA
authors.lastname: Shah
authors.forename: Young-Ryun
authors.initials: YR
authors.lastname: Chung
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
authors.forename: Eui-Sung
authors.initials: ES
authors.lastname: Choi
authors.forename: Jay D
authors.initials: JD
authors.lastname: Keasling
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005204:Farnesol / Q000378:metabolism*; D018628:Gene Dosage; D015870:Gene Expression; D051228:Geranyltranstransferase / Q000235:genetics; D045782:Hemiterpenes / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008798:Mevalonic Acid / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010957:Plasmids; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism
chemical_list: D003470:Culture Media; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D005204:Farnesol; C004808:farnesyl pyrophosphate; D051228:Geranyltranstransferase; D008798:Mevalonic Acid