title: Matrix metalloproteinase-1 expression can be upregulated through mitogen-activated protein kinase pathway under the influence of human epidermal growth factor receptor 2 synergized with estrogen receptor.
abstract: In our previous work, Ets-1 upregulates human epidermal growth factor receptor 2 (HER2) induced matrix metalloproteinase 1 (MMP-1) expression. Based on the above knowledge and result, we hypothesized that estrogen receptor (ER) and its signaling pathway may affect MMP-1 expression under the influence of HER2. In addition, we investigated how the HER2 pathway cross-talk with the ER signaling pathway in genomic and nongenomic action of ER using reverse transcription-PCR, Western blot analysis, and ELISA assay. The results showed that ER-alpha expression increased MMP-1 expression under the presence of HER2. These upregulatory effects were mediated mainly by mitogen-activated protein kinase pathway and were reversed by downregulation of HER2 and/or ER. Activator protein DNA binding activity was involved in the MMP-1 expression. In summary, our results showed that ER can upregulate MMP-1 expression under the influence of HER2 in MCF-7 cells. In addition, this upregulatory effect was found to be mediated by mitogen-activated protein kinase pathway. MMP-1 might be an assigned target in interaction between ER and HER2.
authors.affiliation: Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Hae Hyun
authors.initials: HH
authors.lastname: Jung
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Park
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Jun
authors.forename: Jeehyun
authors.initials: J
authors.lastname: Kong
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Jung A
authors.initials: JA
authors.lastname: Kim
authors.forename: Jina
authors.initials: J
authors.lastname: Yun
authors.forename: Jong Mu
authors.initials: JM
authors.lastname: Sun
authors.forename: Young Woong
authors.initials: YW
authors.lastname: Won
authors.forename: Soohyeon
authors.initials: S
authors.lastname: Lee
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Ahn
authors.forename: Young-Hyuck
authors.initials: YH
authors.lastname: Im
mesh_terms: D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D006801:Humans; D020935:MAP Kinase Signaling System; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D011960:Receptors, Estrogen; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases; D020781:Matrix Metalloproteinase 1