title: A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells.
abstract: MicroRNAs are approximately 22nt non-coding RNAs that are present in a broad range of multicellular organisms. MicroRNAs play important roles in many biological or pathological processes by regulating the expression of their target genes. The fast and accurate identification of miRNA targets is a bottleneck in the clarification of the function of miRNAs. Here, we established a rapid and accurate strategy to identify miRNA functional target genes by combination of bioinformatic prediction with Cytoplasmic/Nuclear (C/N) ratios of mRNAs. The strategy comprises three steps: bioinformatic prediction, determination of mRNA C/N ratios, and confirmation by Western blotting, and might be suitable to most miRNAs. Our method will make a significant contribution to the study of the biological functions of miRNAs.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Baochun
authors.initials: B
authors.lastname: Huang
authors.forename: Liucun
authors.initials: L
authors.lastname: Chen
authors.forename: Xueting
authors.initials: X
authors.lastname: Su
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002477:Cells / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger