A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection.
We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
Zhou
Yuntao
Y
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Huang
Qing
Q
Gao
Jimin
J
Lu
Jianxin
J
Shen
Xizhong
X
Fan
Chunhai
C
D052160:Benzothiazoles; D014408:Biomarkers, Tumor / Q000032:analysis; D015342:DNA Probes / Q000737:chemistry*; D003959:Diamines; D005456:Fluorescent Dyes; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009930:Organic Chemicals; D011804:Quinolines; D013050:Spectrometry, Fluorescence
D052160:Benzothiazoles; D014408:Biomarkers, Tumor; D015342:DNA Probes; D003959:Diamines; D005456:Fluorescent Dyes; D035683:MicroRNAs; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I