title: Glutathione-S-transferase in Nereis succinea (Polychaeta) and its induction by xeno-estrogen.
abstract: The need to replace or at least to reduce the use of vertebrates in toxicity tests is a timely major concern in research and industry but to date, efforts made to minimize their use are still far from complete. Increasing demands for toxicity tests put considerable pressures upon the development of future fast and efficient test methods using invertebrates. In fact, to date, few studies provide links between biochemical and cellular effects of xeno-estrogens in aquatic invertebrates. Glutathione-S-transferase (GST) activity, as a biomarker of stress exposure, was measured in the population of clamworms (Nereis succinea) from Cardiff Bay. In addition, we examined the effect of single exposure to nonylphenol (NP) on this enzymatic activity. Field study results showed a relationship between the worm's size, reproductive status, and GST activity from the field population. In addition, we show a significant increase in the GST activity at 100 μg/L NP with sex-specific responses. The xeno-estrogens, which could affect reproduction of nereid by interfering in normal endocrinological pathways, are eliminated through GST by conjugation with glutathione. This work shows for the first time that GST activity depends on sex and stage of the clamworms and also that the xeno-estrogen NP induces its activity. This study supports the use of this species as a bioindicator of aquatic pollution and lays the foundation to causally link toxic exposure with reproductive output.
authors.affiliation: Department of Biological Sciences, University of Hull, Hull HU67RX, UK.
authors.forename: James A O
authors.initials: JA
authors.lastname: Ayoola
authors.forename: Javier
authors.initials: J
authors.lastname: García-Alonso
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Hardege
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D011077:Polychaeta / Q000187:drug effects* / Q000201:enzymology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D004967:Estrogens; D010636:Phenols; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; C025256:nonylphenol; D005982:Glutathione Transferase