title: In vivo PET imaging and biodistribution of radiolabeled gold nanoshells in rats with tumor xenografts.
abstract: Here we report the radiolabeling of gold nanoshells (NSs) for PET imaging in rat tumor model. A conjugation method was developed to attach NSs with the radionuclide, (64)Cu. The resulting conjugates showed good labeling efficiency and stability in PBS and serum. The pharmacokinetics of (64)Cu-NS and the controls ((64)Cu-DOTA and (64)Cu-DOTA-PEG2K) were determined in nude rats with a head and neck squamous cell carcinoma xenograft by radioactive counting. Using PET/CT imaging, we monitored the in vivo distribution of (64)Cu-NS and the controls in the tumor-bearing rats at various time points after their intravenous injection. PET images of the rats showed accumulation of (64)Cu-NSs in the tumors and other organs with significant difference from the controls. The organ biodistribution of rats at 46h post-injection was analyzed by radioactive counting and compared between the (64)Cu-NS and the controls. Different clearance kinetics was indicated. Neutron activation analysis (NAA) of gold concentration was performed to quantify the amount of NSs in major tissues of the dosed rats and the results showed similar distribution. Overall, PET images with (64)Cu had good resolution and therefore can be further applied to guide photothermal treatment of cancer.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, 3100 Cleburn St, Houston, TX 77004, United States. xieh@tsu.edu
authors.forename: Huan
authors.initials: H
authors.lastname: Xie
authors.forename: Zheng Jim
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Ande
authors.initials: A
authors.lastname: Bao
authors.forename: Beth
authors.initials: B
authors.lastname: Goins
authors.forename: William T
authors.initials: WT
authors.lastname: Phillips
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging* / Q000378:metabolism; D045744:Cell Line, Tumor; D003301:Copper Radioisotopes / Q000008:administration & dosage / Q000493:pharmacokinetics; D005240:Feasibility Studies; D006046:Gold; D006258:Head and Neck Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D006801:Humans; D007275:Injections, Intravenous; D053768:Metal Nanoparticles / Q000008:administration & dosage; D009368:Neoplasm Transplantation; D009501:Neutron Activation Analysis; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000493:pharmacokinetics; D051381:Rats; D011923:Rats, Nude; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: D003301:Copper Radioisotopes; D019275:Radiopharmaceuticals; D006046:Gold