Immunogenicity of multiple-epitope antigen gene of HCV carried by novel biodegradable polymers.
In order to develop a promising vaccine candidate utilizing a combined approach to induce both antibody production and T-cell activity, the DNA fragment containing MA of HCV with five conserved epitopes was synthesized. Two types of HCV vaccine candidates (the DNA type and DNA/polymers) were constructed using MA. PLA-PEG-PLA and PLGA-PEG-PLGA were synthesized and used as micelles with encapsulated plasmid pcDNA3.1(+)-MA. The preparation of copolymers, the cloning and analysis of recombinant plasmid DNA, in vitro expression, and immunogenicity in transgenic mice were evaluated in detail. The results indicated that even single immunization and oral immunization with DNA/polymers achieved satisfying immune responses in vivo tests. As biodegradable and nontoxic triblock copolymers, the novel copolymers demonstrated a great advantage, as they made long-term and single-immunizing vaccines possible; in addition, the copolymers showed a better adjuvant effect and scarcely any side effects.
Yang
Yuan
Y
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
Kuang
Yu
Y
Liu
Yu
Y
Li
Wanyi
W
Jiang
Zhonghua
Z
Xiao
Liying
L
Li
Mingyuan
M
D000818:Animals; D000939:Epitopes / Q000235:genetics*; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000517:prevention & control; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D011092:Polyethylene Glycols / Q000378:metabolism*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology*
D000939:Epitopes; D019444:Vaccines, DNA; C542623:monomethoxypolyethyleneglycol-polylactide block copolymer; D011092:Polyethylene Glycols