A simple and efficient method for isolation of a single Eimeria oocyst from poultry litter using a micromanipulator.
The chicken, which is the host for seven species of Eimeria, typically is infected simultaneously by multiple Eimeria species and the oocysts of coccidia are excreted in the feces. A prerequisite for investigation of individual Eimeria species is to isolate a single oocyst from fecal samples. A novel method for isolating a single Eimeria oocyst from poultry litter using a micromanipulator was developed. This simple method is fast and reliable, and provides direct isolation of a single sporulated oocyst from fecal samples harboring multiple Eimeria species or samples contaminated by other species of parasite.
Yim
Dongjean
D
College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-701, South Korea.
Kang
Sang S
SS
Lillehoj
Hyun S
HS
Min
Wongi
W
D000818:Animals; D002645:Chickens; D004539:Eimeria / Q000166:cytology / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D005430:Floors and Floorcoverings; D006799:Housing, Animal; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D033661:Oocysts / Q000166:cytology*