Monitoring biofilm development in a microfluidic device using modified confocal reflection microscopy.
The feasibility of a method to monitor biofilm development non-destructively in a microfluidic device was addressed. Here, we report that biofilm growth could be non-destructively monitored by an image analysis technique based on modification of confocal reflection microscopy.
Yawata
Yutaka
Y
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennnoudai1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
Toda
Kensuke
K
Setoyama
Erika
E
Fukuda
Junji
J
Suzuki
Hiroaki
H
Uchiyama
Hiroo
H
Nomura
Nobuhiko
N
D058491:Bacterial Load / Q000295:instrumentation / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D018929:Cell Culture Techniques; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D013295:Streptococcus mutans / Q000166:cytology* / Q000254:growth & development*