Tissue expression of interleukin 2 (IL-2) and IL-2 receptor (IL-2R(alpha)/CD25) in non-Hodgkin B-cell lymphomas in children: correlations with clinical data.
The study aimed at analysis of tissue expression of interleukin 2 (IL-2) and subunit alpha of its receptor (IL-22R(alpha)/CD25) in B-cell non-Hodgkin lymphomas (B-cell NHLs) in children as related to selected clinical data. The studies were performed on an archival tissue material originating from children with B-cell NHLs (n=26) using avidin-biotin-peroxidase complex method and in-situ hybridization with biotinylated tyramine amplification signal (ImmunoMax technique). As compared with non-neoplastic lesions, in B-cell NHLs a significantly higher expression was noted of both IL-2 and IL-22R(alpha). In children with B-cell NHLs, frequency of IL-2 detection was 62%, and that of IL-22R(alpha), 46%. Using hybridocytochemistry, presence of mRNA for IL-2 could be detected, but not mRNA for IL-22R(alpha) in children with B-cell NHLs. A significant direct relationship was demonstrated between expressions of IL-2 and IL-22R(alpha) in children with B-cell NHLs. No significant relationships could be detected between expression of IL-2 and/or IL-22R(alpha) on one hand and histopathologic diagnosis, tumor location, age, or sex in B-cell NHLs on the other. The new element in the results involved demonstration of significant differences in shorter survival of children with B-cell NHLs, as related to cellular expression of IL-2 and IL-22R(alpha) (CD25). Comparing the expected percentage of patients at risk, children with CD25-positive tissue expression had a significantly lower chance to survive longer time than children with IL-2-positive tissue expression. The positive relationship between tissue expression of IL-22R(alpha) and the shorter survival of children with B-cell NHLs should prompt further investigations to identify other risk factors in patients with this type of tumors in children.
Kasprzak
Aldona
A
Department of Histology and Embryology, Poznan University of Medical Sciences, Poznań, Poland. akasprza@ump.edu.pl
Spachacz
Rafał
R
Wachowiak
Jacek
J
Stefańska
Katarzyna
K
Kaczmarek
Elzbieta
E
Zabel
Maciej
M
D000293:Adolescent; D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D007376:Interleukin-2 / Q000096:biosynthesis*; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis*; D053208:Kaplan-Meier Estimate; D016393:Lymphoma, B-Cell / Q000378:metabolism* / Q000401:mortality; D008297:Male
D014408:Biomarkers, Tumor; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit