title: Blocking effect of a monoclonal antibody against recombinant Pvs25 on sporozoite development in Anopheles sinensis.
abstract: To develop a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface protein Pvs25 was cloned from a Korean malaria patient. The Pvs25 gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquito Anopheles sinensis, which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.
authors.affiliation: Department of Pathology, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610, USA.
authors.forename: Sung-Ung
authors.initials: SU
authors.lastname: Moon
authors.forename: Hyung-Hwan
authors.initials: HH
authors.lastname: Kim
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Choi
authors.forename: Chang-Mi
authors.initials: CM
authors.lastname: Oh
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Seo-Kyoung
authors.initials: SK
authors.lastname: Hwang
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Sohn
authors.forename: E-Hyun
authors.initials: EH
authors.lastname: Shin
authors.forename: Hyuck
authors.initials: H
authors.lastname: Kim
authors.forename: Hyeong-Woo
authors.initials: HW
authors.lastname: Lee
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004199:Disease Vectors; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017780:Malaria Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016780:Malaria, Vivax / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D034101:Sporozoites / Q000254:growth & development / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017780:Malaria Vaccines; C121473:Pvs25 protein, P vivax; D011994:Recombinant Proteins