Gastroenteric virus detection in fecal samples from women in Goiânia, State of Goiás, Brazil.
BACKGROUND
This was a prospective study that included women seen in the obstetrics and gynecology sector of Hospital das Clínicas, Federal University of Goiás, in Goiânia, State of Goiás, with the aim of detecting rotaviruses, adenoviruses, caliciviruses and astroviruses. Eighty-four women participated in the study and from these, 314 fecal samples were collected. Out of all of the women, 29 were seropositive for HIV and 55 were seronegative, and 45 and 39 were pregnant and non-pregnant, respectively.


METHODS
Fecal samples were collected from each woman once every two months over the period from July 2006 to June 2007, and they were screened for rotaviruses by means of polyacrylamide gel electrophoresis and immunoenzymatic assays, for caliciviruses and astroviruses by means of RT-PCR and for adenovirus by means of immunoenzymatic assays. The astroviruses were genotyped using nested PCR.


RESULTS
Among the 84 patients, 19 (22.6%) were positive for either calicivirus (14/19) or astrovirus (6/19), while one women was positive for both viruses in fecal samples collected on different occasions. Most of the positive samples were collected during the months of July and August (astrovirus) and September and October (calicivirus). None of the samples analyzed was positive for rotavirus or adenovirus. Gastroenteric viruses were detected in 13/19 (68.4%) of the pregnant women, whether HIV-seropositive or not.


CONCLUSIONS
The results from the present study showed that neither pregnancy nor HIV-seropositive status among the women increased the risk of infection by any of the gastroenteric viruses studied. This study presents data on gastroenteric virus detection among pregnant and/or HIV-positive women.
Ferreira
Rui Gilberto
RG
Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, GO, Brazil. ruigilberto@fertile.com.br
Borges
Ana Maria Tavares
AM
Fiaccadori
Fabiola Souza
FS
Souza
Menira Borges de Lima Dias E
MB
Santos
Rodrigo Alessandro Togo
RA
Cardoso
Divina das Dôres de Paula
Dd
D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000256:Adenoviridae / Q000302:isolation & purification*; D000328:Adult; D001938:Brazil; D002139:Caliciviridae / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D001257:Mamastrovirus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D015995:Prevalence; D011446:Prospective Studies; D012328:RNA Viruses / Q000145:classification / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification; D055815:Young Adult