Single layer centrifugation of stallion spermatozoa through Androcoll™-E does not adversely affect their capacitation-like status, as measured by CTC staining.
This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation-like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6°C. Sperm capacitation-like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation-like status between colloid-selected and non-selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome-reacted spermatozoa. In conclusion, SLC through Androcoll™-E does not adversely affect the capacitation-like status of stallion spermatozoa, although it did increase with time during cold storage.
Bergqvist
A-S
AS
Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Johannisson
A
A
Bäckgren
L
L
Dalin
A-M
AM
Rodriguez-Martinez
H
H
Morrell
J M
JM
D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002469:Cell Separation / Q000379:methods / Q000662:veterinary; D002498:Centrifugation / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D002751:Chlortetracycline; D003080:Cold Temperature; D003102:Colloids; D005456:Fluorescent Dyes; D006736:Horses; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013075:Sperm Capacitation / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
D003102:Colloids; D005456:Fluorescent Dyes; D002751:Chlortetracycline