title: Viable fertile mice generated from fully pluripotent iPS cells derived from adult somatic cells.
abstract: Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic origin. Achieving fully pluripotent iPS cells from multiple cell types, especially easily accessible adult tissues, will lead to a much greater clinical impact. We successfully generated high-pluripotency iPS cells from adult tail tip fibroblasts (TTF) that resulted in viable, full-term, fertile TTF-iPS animals with no obvious teratoma formation or other developmental abnormalities. Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies. This work provides the means for studying the mechanisms and regulation of direct reprogramming, and has encouraging implications for future clinical applications and therapeutic interventions.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiao-yang
authors.initials: XY
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lv
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Man
authors.initials: M
authors.lastname: Tong
authors.forename: Tang
authors.initials: T
authors.lastname: Hai
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Fanyi
authors.initials: F
authors.lastname: Zeng
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D019976:Cloning, Organism / Q000379:methods*; D005298:Fertility / Q000502:physiology; D005328:Fetal Viability / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic / Q000502:physiology; D008954:Models, Biological