title: [The estimation of the number of platelets by different methods].
abstract: The commonly available and accurate platelet counts play an important role in the evaluation of haemorrhagic status and in assessing the need for platelet transfusions. We evaluated platelet counting performance by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometry using optical, impedance and immunological methods in trombocytopenic patients. Hematology analysers display the high precision and the accuracy of the measurement of the number of platelets in whole blood. However, the measurement of number of platelets can be disturbed by the presence of interference factors such as: microcytic erytrocytes, schizocytes, cryoglobulins, microplatelets and immunological clumps.
  
  
  OBJECTIVE
  The comparison of impedance, optical and immunological enumeration methods of platelets by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometer.
  
  
  RESULTS
  The platelet count of all considered methods showed good correlation with the cytometric method.
  
  
  CONCLUSIONS
  Only cytometric method with using anti-CD41 monoclonal antibody allowed to verify the results of enumeration methods of platelets.
authors.affiliation: Uniwersytet Medyczny w Bialymstoku, Zakład Laboratoryjnej Diagnostyki Pediatrycznej. karol.cam@interia.pl
authors.forename: Karol
authors.initials: K
authors.lastname: Ratomski
authors.forename: Janusz
authors.initials: J
authors.lastname: Zak
authors.forename: Edwina
authors.initials: E
authors.lastname: Kasprzycka
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hryniewicz
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wysocka
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006405:Hematology / Q000295:instrumentation / Q000379:methods; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010976:Platelet Count / Q000379:methods*; D011237:Predictive Value of Tests; D012016:Reference Values