Bioluminescence imaging of dual gene expression at the single-cell level.
Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.
Kwon
Hyuckjoon
H
Regenerative Medicine/Tissue Engineering Division, Research Center for Cooperative Projects, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Enomoto
Toshiteru
T
Shimogawara
Masahiro
M
Yasuda
Kazunori
K
Nakajima
Yoshihiro
Y
Ohmiya
Yoshihiro
Y
D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics; D014162:Transfection
D011993:Recombinant Fusion Proteins; D008156:Luciferases