title: UbcH10 expression on thyroid fine-needle aspirates.
abstract: BACKGROUND
  Thyroid fine-needle aspiration (FNA) samples belonging to the follicular neoplasm/suspicious for malignancy classes are controversial. The authors identified UbcH10 as a marker useful in the diagnosis of several neoplasms, including thyroid cancer. Here, analysis of UbcH10 expression by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry was applied to FNAs.
  
  
  METHODS
  A series of 84 follicular neoplasm/suspicious for malignancy FNAs with histological follow-up (30 malignant) was prospectively collected. UbcH10 immunostaining was performed on cell blocks and compared with that of the proliferation marker Ki-67. At the mRNA level, UbcH10 was compared with CCND2 and PCSK2 expression, these latter being the best performing components of the previously reported 3-gene assay; to determine the diagnostic accuracy, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve for each gene individually and in combination was evaluated.
  
  
  RESULTS
  UbcH10 and Ki-67 shared a similar pattern; although UbcH10 expression was higher in malignant than in benign lesions (P < .001), staining was sporadic, and the cutoff value derived by the ROC analysis was too low (1.25%) for routine application. Conversely, UbcH10 expression assessment by quantitative RT-PCR was effective. UbcH10 mRNA levels associated with malignant histology were significantly higher than those associated with benign histology (P = .02). The AUC was 0.74 for UbcH10, 0.81 for CCDN2, 0.62 for PCSK2, and 0.84 for UbcH10 and CCND2 combination.
  
  
  CONCLUSIONS
  UbcH10 quantitative RT-PCR analysis, rather than immunohistochemistry, is useful to increase the detection of malignancy in thyroid FNAs. UbcH10 may be added as a panel component in quantitative RT-PCR-based assays.
authors.affiliation: Department of Biomorphology Sciences, Faculty of Medicine and Surgery, University of Naples Federico II, Naples, Italy.
authors.forename: Eliana
authors.initials: E
authors.lastname: Guerriero
authors.forename: Angelo
authors.initials: A
authors.lastname: Ferraro
authors.forename: Doriana
authors.initials: D
authors.lastname: Desiderio
authors.forename: Pierlorenzo
authors.initials: P
authors.lastname: Pallante
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Berlingieri
authors.forename: Antonino
authors.initials: A
authors.lastname: Iaccarino
authors.forename: Emiliano
authors.initials: E
authors.lastname: Palmieri
authors.forename: Lucio
authors.initials: L
authors.lastname: Palombini
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D044963:Biopsy, Fine-Needle; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000235:genetics; D044763:Ubiquitin-Conjugating Enzymes / Q000032:analysis* / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D012333:RNA, Messenger; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes