title: Effects of Wnt-10b on proliferation and differentiation of adult murine skin-derived CD34 and CD49f double-positive cells.
abstract: Although mouse Wnt-10b has been shown to play various roles in a wide range of biological actions, the effects on epithelial stem/progenitor cells in the skin have not been reported. In the present study, we investigated the effects of Wnt-10b on proliferation and differentiation of murine skin-derived CD34 and CD49f double-positive (CD34(+)CD49f(+)) cells, a supposed fraction as enriched epithelial stem/progenitor cells. The cells were prepared from dorsal skin samples obtained from young adult mice as alpha6 integrin (CD49f) and CD34 double-positive cells by fluorescent activated cell sorting (FACS), and they were cultured with or without Wnt-10b to investigate its effects on proliferation and differentiation. Involvement of canonical Wnt signaling pathway was confirmed by TOPFLASH assay, and differentiation of the CD34(+)CD49f(+) cells was assessed by RT-PCR analysis and immunocytochemical examinations. The skin-derived CD34(+)CD49f(+) cells were immunopositive for Lhx2 and expressed mRNA of classical markers for bulge stem cells, including Lhx2, keratin15, Sox9, S100a6, and NFATc1. Their proliferation was suppressed by Wnt-10b, and the markers for differentiated epithelial cells became to be expressed in the culture with Wnt-10b. These results suggest that Wnt-10b promotes differentiation of epithelial stem/progenitor cells in the skin.
authors.affiliation: Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. oujix@naramed-u.ac.jp
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Mariko
authors.initials: M
authors.lastname: Nishiofuku
authors.forename: Noriko
authors.initials: N
authors.lastname: Ouji-Sageshima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kubo
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ishizaka
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D049108:Cell Enlargement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D039503:Integrin alpha6 / Q000378:metabolism*; D051379:Mice; D012867:Skin / Q000166:cytology* / Q000187:drug effects; D012879:Skin Physiological Phenomena / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051153:Wnt Proteins / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D039503:Integrin alpha6; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse