title: Rat monoclonal antibody specific for MyoD.
abstract: Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.
authors.affiliation: Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Harada
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: Shinpei
authors.initials: S
authors.lastname: Ao
authors.forename: Jun
authors.initials: J
authors.lastname: Odawara
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Mako
authors.initials: M
authors.lastname: Nakamura
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D022041:Euchromatin / Q000276:immunology; D005260:Female; D006825:Hybridomas; D007082:Ileum / Q000276:immunology; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000254:growth & development / Q000276:immunology*; D017570:MyoD Protein / Q000276:immunology*; D032446:Myoblasts / Q000276:immunology* / Q000648:ultrastructure; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D000911:Antibodies, Monoclonal; D022041:Euchromatin; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein