title: [Cartilage tissue reconstruction by the polymer biomaterials--early macroscopic and histological results].
abstract: BACKGROUND
  The surgical treatment of large cartilage defects in the region of head and neck is often impossible because of the atrophy of surrounding tissues and lack of suitable material for reconstruction. In the surgical treatment many of methods and reconstructive materials have been used. For many years the suitable synthetic material for the cartilage defects reconstruction has been searched for.
  
  
  OBJECTIVE
  Was to evaluate two different biomaterials with proper mechanical and biological features for the cartilage replacement.
  
  
  METHODS
  Two type of biomaterials in this study were used: resorbable polymer - poly(L-lactide-co-glycolide) (PLG) acting as a supportive matrix. A thin layer of sodium hyaluronate (Hyal) was also deposited on the surface as well in the pore walls of PLG scaffolds in order to provide biologically active molecules promoting differentiation and regeneration of the tissue. The studies were performed on the 50 animals--rabbits divided into 2 groups. The animals were operated in the general anaesthesia. The incision was done along the edge of the rabbit's auricle. Perichondrium and cartilage of the auricle on the surface 4 x 3 cm were prepared. Subperichondrically 1 x 1 cm fragment of the cartilage was removed by the scissors. This fragment was then replaced by the biomaterials: PLG in first group of 25 rabbits and PLG-Hyal in second group 25 rabbits. The tissues were sutured with polyglycolide Safil 3-0. The animals obtained Enrofloxacin for three days after the operation. Then 1, 4 and 12 weeks after the surgery the animals were painlessly euthanized by an overdose of Morbital. Implants and surrounding tissues were excised and observed macroscopically and using an optical microscope.
  
  
  RESULTS
  In all the observation periods we observed proper macroscopic healing process of biomaterials. We didn't stated strong inflammatory process and necrosis around the implanted biomaterials.
  
  
  CONCLUSIONS
  The histological and macroscopic examinations indicated that both materials developed in this study have properties similar to cartilaginous tissue and seem to be good for her restoration. Although the quickest tissue regeneration was found after implantation of PLG-Hyal.
authors.affiliation: Katedra i Oddział Kliniczny Laryngologii w Zabrzu Slaskiego Uniwersytetu Medycznego w Katowicach. wojscier@mp.pl
authors.forename: Wojciech
authors.initials: W
authors.lastname: Scierski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Polok
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Namysłowski
authors.forename: Jerzy
authors.initials: J
authors.lastname: Nozyński
authors.forename: Lucyna
authors.initials: L
authors.lastname: Turecka
authors.forename: Natalia
authors.initials: N
authors.lastname: Urbaniec
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pamuła
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D002356:Cartilage / Q000637:transplantation*; D002454:Cell Differentiation / Q000187:drug effects; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D011107:Polylysine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D011138:Polytetrafluoroethylene / Q000627:therapeutic use*; D011817:Rabbits; D019651:Plastic Surgery Procedures / Q000295:instrumentation* / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D011107:Polylysine; D011099:Polyglutamic Acid; C030391:poly(glutamic acid-lysine); D011138:Polytetrafluoroethylene; D006820:Hyaluronic Acid