Induction of Foxp3 expression in T cells by cellulose acetate beads in vitro.
Gene expression of transforming growth factor-beta (TGF-beta) is needed to induce expression of transcription factor forkhead box P3 (Foxp3), which is required for the development and function of regulatory T (Treg) cells. The number of circulating Treg cells and the level of Foxp3 expression increase during granulocyte and monocyte apheresis (GMA), a useful therapy for ulcerative colitis. However, the mechanism underlying GMA-induced Foxp3 expression is unknown. We found that the level of TGF-beta mRNA in peripheral blood mononuclear cells (PBMCs) was augmented just after treatment of peripheral blood with a GMA carrier, cellulose acetate beads, in vitro and that Foxp3 expression in PBMCs increased after culturing these cells for 5 days after the treatment. The augmentation of TGF-beta expression was observed in CD3(-) PBMCs but not in CD3(+) T cells. Furthermore, the increase in Foxp3 expression in T cells depended on co-culture with CD3(-) PBMCs. We conclude that cellulose acetate beads have an ability to induce Foxp3 expression in peripheral blood T cells via augmentation of TGF-beta expression in CD3(-) PBMCs.
Jimma
Fumie
F
JIMRO Co., Ltd., Research Division, 351-1 Nishiyokote-cho, Takasaki, Gunma 370-0021, Japan.
Takeda
Yuji
Y
Kaneda
Kenta
K
Wakabayashi
Ichiro
I
D001781:Blood Component Removal / Q000379:methods; D017252:CD3 Complex; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D018920:Coculture Techniques; D003093:Colitis, Ulcerative / Q000628:therapy; D051858:Forkhead Transcription Factors / Q000235:genetics*; D006098:Granulocytes; D006801:Humans; D016130:Immunophenotyping; D008863:Microspheres; D009000:Monocytes; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000187:drug effects
D017252:CD3 Complex; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D016212:Transforming Growth Factor beta; C005062:acetylcellulose; D002482:Cellulose