title: [Expression of mast cell tryptase in scar].
abstract: OBJECTIVE
  To investigate the expression and distribution of mast cell tryptase (MCT) in scar, and to discuss the different MCT gene expression in keloid, hypertrophic scar and normal skin.
  
  
  METHODS
  20 samples of keloid, 20 samples of hypertrophic scar and 20 samples of normal skin were collected. The distribution of MCT was investigated by immunofluorescence histochemistry, and the MCT mRNA expression was detected by Relative Quantification real-time fluorescent PCR.
  
  
  RESULTS
  MCT gene was mainly located in the collagen fiber bundles of the scar, especially in the superficial layer of scar. MCT mRNA expression was significantly higher in keloid than that in hypertrophic scar and normal skin (P < 0.01). Averagely, the MCT gene expression in keloid was 2.5 times and 5.4 times of that in hypertrophic scar and normal skin.
  
  
  CONCLUSIONS
  MCT gene may play a role in the pathogenesis of scar.
authors.affiliation: Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Feng
authors.forename: Ran
authors.initials: R
authors.lastname: Huo
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Xue
authors.forename: Fa-gang
authors.initials: FG
authors.lastname: Wang
authors.forename: Ren-rong
authors.initials: RR
authors.lastname: Lv
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000378:metabolism / Q000473:pathology; D053802:Tryptases / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D053802:Tryptases