title: Difference in the level of interferon gamma mRNA transcripts on stimulation of cattle and buffalo mononuclear cells with foot and mouth disease virus-antigen: a possible role of sequence variation in promoter region.
abstract: In an attempt to resolve the claim that buffaloes differ from cattle in disease progression, this study was undertaken to compare the mitogen (conA) or antigen (foot and mouth disease virus) induced expression levels of interferon gamma (IFN-γ mRNA in peripheral blood mononuclear cells (PBMCs) by real-time quantitative PCR. In general, the levels of IFN-γ mRNA were lower in buffaloes than in crossbred cattle. Significantly higher levels of IFN-γ mRNA were also observed in crossbred cattle when induced with FMD virus (1 μg). Analysis of the partial promoter sequences of the IFN-γ gene from the respective species revealed a conserved 4 base (GTCT) deletion in all the buffalo promoter sequences. In-silico analysis indicated the binding of glucocorticoid receptor (GR) and erythroid nuclear factor (NF-E) to this region in cattle. GR has been shown to be a transcription factor by itself and also regulates other major transcription factors like NF-κB and AP-1. The differential expression levels of IFN-γ mRNA between these species could be due to this deletion in the promoter region of buffalo. Further studies involving mobility shift and promoter assays would throw more light on the differential expression levels.
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600007, India.
authors.forename: B
authors.initials: B
authors.lastname: Mohana Subramanian
authors.forename: S
authors.initials: S
authors.lastname: Senthuran
authors.forename: G
authors.initials: G
authors.lastname: Dhinakar Raj
authors.forename: K G
authors.initials: KG
authors.lastname: Tirumurugaan
authors.forename: D
authors.initials: D
authors.lastname: Thiagarajan
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000494:pharmacology*; D002020:Buffaloes / Q000235:genetics / Q000276:immunology*; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000956:Antigens, Viral; D012333:RNA, Messenger; D007371:Interferon-gamma