Accumulation of neurons differentiated from mouse embryonic stem cells in particular areas of culture plate surface.
Nanoscale magnetic beads coated with nerve growth factor (NGF) allow us to accumulate neurons differentiated from mouse ES cells in a selected area of the culture plate surface using a magnet. Neurons with neurite outgrowths within a particular area expressed TrkA and incorporated beads in the soma.
Kitazawa
Ayako
A
Bio-Nano Electronics Research Center, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan.
Naka
Yukie
Y
Yamaguchi
Hiroko
H
Shimizu
Norio
N
D000818:Animals; D019149:Bioreactors; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation*