title: Dried fruit extract from Xylopia aethiopica (Annonaceae) protects Wistar albino rats from adverse effects of whole body radiation.
abstract: The effect of dried fruit extract from Xylopia aethiopica (Annonaceae) (XA) and vitamin C (VC) against γ-radiation-induced liver and kidney damage was studied in male Wistar rats. XA and VC were given orally at a dose of 250 mg/kg, orally for 6 weeks prior to and 8 weeks after radiation (5 Gy). The rats were sacrificed after 1 and 8 weeks of single exposure to radiation. Results showed that all animals in un-irradiated group survived (100%), while 83.3% and 66.7% survived in XA- and VC-treated groups, respectively, and 50% survived in irradiated group. The levels of serum, liver and kidney lipid peroxidation (LPO) were elevated by 88%, 102% and 73% after 1 week of exposure, and by 152%, 221% and 178%, after 8 weeks of exposure, respectively. Treatment with XA and VC significantly (p<0.05) decreased the levels of LPO in the irradiated animals. Also, γ-radiation caused significant decreases (p<0.05) in the levels of liver glutathione (GSH), glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), kidney GSH and SOD by 41%, 60%, 81%, 79%, 72% and 58% after 1 week of exposure. Similarly, γ-radiation caused significant increases (p<0.05) in the levels of serum alanine (ALT) and aspartate aminotransferases (AST) after 8 weeks of exposure. Precisely, ALT and AST levels were increased by 69% and 82%, respectively. These changes were significantly (p<0.05) attenuated in irradiated animals treated with XA and VC. These results suggest that XA and VC could increase the antioxidant defence systems in the liver and kidney of irradiated animals, and may protect from adverse effects of whole body radiation.
authors.affiliation: Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria. aoadaramoye@yahoo.com
authors.forename: Oluwatosin A
authors.initials: OA
authors.lastname: Adaramoye
authors.forename: Osume O
authors.initials: OO
authors.lastname: Okiti
authors.forename: E Olatunde
authors.initials: EO
authors.lastname: Farombi
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage; D001205:Ascorbic Acid / Q000008:administration & dosage; D001219:Aspartate Aminotransferases / Q000097:blood; D004359:Drug Therapy, Combination; D005638:Fruit / Q000737:chemistry; D005720:Gamma Rays / Q000009:adverse effects*; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D010088:Oxidoreductases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001205:Ascorbic Acid