title: Hybrid PET/CT for noninvasive pharmacokinetic evaluation of dynamic PolyConjugates, a synthetic siRNA delivery system.
abstract: Positron emission tomography/computed tomography (PET/CT) hybrid imaging can be used to gain insights into a synthetic siRNA delivery system targeted to the liver. Either siRNA or the delivery vehicle was labeled with (64)Cu via 1, 4, 7, 10- tetraazacyclododecane- 1, 4, 7, 10- tetraacetic acid (DOTA) chelation. This study confirmed that the siRNA delivery system was successfully targeted to the liver. Incorporation of the siRNA into the delivery system protected the siRNA from renal filtration long enough so that the siRNA could be delivered to the liver. PET/CT imaging was important for confirming biodistribution and for determining differences in the distribution of labeled siRNA, siRNA incorporated into the delivery system, and the delivery system without siRNA.
authors.affiliation: Department of Pharmaceutical Sciences, University of Wisconsin - Madison, Madison, Wisconsin 53705, USA.
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Mudd
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Trubetskoy
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Blokhin
authors.forename: Jamey P
authors.initials: JP
authors.lastname: Weichert
authors.forename: Jon A
authors.initials: JA
authors.lastname: Wolff
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D003287:Contrast Media / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D006573:Heterocyclic Compounds, 1-Ring / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism*; D051379:Mice; D049268:Positron-Emission Tomography / Q000379:methods*; D034741:RNA, Small Interfering / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed
chemical_list: D002614:Chelating Agents; D003287:Contrast Media; D006573:Heterocyclic Compounds, 1-Ring; D034741:RNA, Small Interfering; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid