title: TIM1 is an endogenous ligand for LMIR5/CD300b: LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury.
abstract: Leukocyte mono-immunoglobulin (Ig)-like receptor 5 (LMIR5)/CD300b is a DAP12-coupled activating receptor predominantly expressed in myeloid cells. The ligands for LMIR have not been reported. We have identified T cell Ig mucin 1 (TIM1) as a possible ligand for LMIR5 by retrovirus-mediated expression cloning. TIM1 interacted only with LMIR5 among the LMIR family, whereas LMIR5 interacted with TIM4 as well as TIM1. The Ig-like domain of LMIR5 bound to TIM1 in the vicinity of the phosphatidylserine (PS)-binding site within the Ig-like domain of TIM1. Unlike its binding to TIM1 or TIM4, LMIR5 failed to bind to PS. LMIR5 binding did not affect TIM1- or TIM4-mediated phagocytosis of apoptotic cells, and stimulation with TIM1 or TIM4 induced LMIR5-mediated activation of mast cells. Notably, LMIR5 deficiency suppressed TIM1-Fc-induced recruitment of neutrophils in the dorsal air pouch, and LMIR5 deficiency attenuated neutrophil accumulation in a model of ischemia/reperfusion injury in the kidneys in which TIM1 expression is up-regulated. In that model, LMIR5 deficiency resulted in ameliorated tubular necrosis and cast formation in the acute phase. Collectively, our results indicate that TIM1 is an endogenous ligand for LMIR5 and that the TIM1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.
authors.affiliation: Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Yamanishi
authors.forename: Jiro
authors.initials: J
authors.lastname: Kitaura
authors.forename: Kumi
authors.initials: K
authors.lastname: Izawa
authors.forename: Ayako
authors.initials: A
authors.lastname: Kaitani
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Komeno
authors.forename: Masaki
authors.initials: M
authors.lastname: Nakamura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Enomoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Oki
authors.forename: Hisaya
authors.initials: H
authors.lastname: Akiba
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.forename: Tadasuke
authors.initials: T
authors.lastname: Komori
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takai
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Toshio
authors.initials: T
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D003001:Cloning, Molecular; D000072596:Hepatitis A Virus Cellular Receptor 1; D007668:Kidney / Q000378:metabolism* / Q000473:pathology*; D007684:Kidney Tubules / Q000473:pathology; D008024:Ligands; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry / Q000172:deficiency* / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: C442240:Havcr1 protein, mouse; D000072596:Hepatitis A Virus Cellular Receptor 1; C527073:LMIR5 protein, mouse; D008024:Ligands; D008565:Membrane Proteins; D010718:Phosphatidylserines; D011971:Receptors, Immunologic