Impact of polymorphism of the regulatory subunit of the μ-calpain (CAPN1S) on the proteolysis process and meat tenderness of young cattle.
The objective of this study was to estimate the impact of the polymorphism of μ-calpain (CAPN1S) gene on protein changes of the cattle muscle tissue and its tenderness during 10-day cold storage. The analysis was performed on the longest dorsal and lumbar muscles collected from 76 bulls 6 to 12 months of age. Polymorphism identification of the above-mentioned gene was conducted using the PCR-RFLP technique. Its effect on the course of the proteolysis process was assessed by monitoring changes in proportions of tissue proteins during 10-day process of meat ageing. Special attention was focused on changes in native titin (T1) share and products of its degradation (proteins of molecular weight (m.w.) of 2400 and 200 kDa), α-actinin and protein of 37 kDa as well as myosin heavy chains (MHC). In the case of the last proteins, their polymorphism was evaluated as well. Meat tenderness was estimated measuring the value of shear force and sensorially. The highest tenderness was ascertained for the heterozygote. Its improvement was associated with a significant decrease in proportions of proteins of molecular weight of approximately 37 kDa accompanied by an increase of those with 200 kDa molecular weight. Muscles derived from cattle of CT genotype were characterised by the highest proportions of type 2a MHC isoform. Value differences between proportions determined for the heterozygote and CC and TT homozygotes of the CAPN1S gene were statistically significant. Therefore, it can be presumed that the process of meat tenderisation was especially connected with MHC polymorphism.
Iwanowska
Agnieszka
A
Institute of Meat Technology, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624, Poznan, Poland.
Grześ
Bożena
B
Mikołajczak
Beata
B
Iwańska
Ewa
E
Juszczuk-Kubiak
Edyta
E
Rosochacki
Stanisław J
SJ
Pospiech
Edward
E
D000818:Animals; D002154:Calpain / Q000235:genetics*; D002417:Cattle; D005838:Genotype; D006579:Heterozygote; D008460:Meat; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D011110:Polymorphism, Genetic; D020033:Protein Isoforms; D013997:Time Factors
D020033:Protein Isoforms; D002154:Calpain; C449002:mu-calpain; D018995:Myosin Heavy Chains