Applying dimorphic yeasts as model organisms to study mycelial growth: part 1. Experimental investigation of the spatio-temporal development of filamentous yeast colonies.
Colony development of the dimorphic yeasts Yarrowia lipolytica and Candida boidinii on solid agar substrates under glucose limitation served as a model system for mycelial development of higher filamentous fungi. Strong differences were observed in the behaviour of both yeasts: C. boidinii colonies reached a final colony extension which was small compared to the size of the growth field. They formed cell-density profiles which steeply declined along the colony radius and no biomass decay processes could be detected. The stop of colony extension coincided with the depletion of glucose from the growth substrate. These findings supported the hypothesis that glucose-limited C. boidinii colonies can be regarded as populations of single cells which grow according to a diffusion-limited growth mechanism. Y. lipolytica colonies continued to extend after the depletion of the primary nutrient resource, glucose, until the populations covered the entire growth field which was accomplished by utilization of mycelial biomass.
Walther
Thomas
T
Institute of Food Technology and Bioprocess Engineering, Technical University Dresden, 01062 Dresden, Germany. thomas.walther@insa-toulouse.fr
Reinsch
Holger
H
Weber
Philipp
P
Ostermann
Kai
K
Deutsch
Andreas
A
Bley
Thomas
T
D000362:Agar; D002175:Candida / Q000166:cytology / Q000254:growth & development*; D005947:Glucose / Q000378:metabolism; D025301:Hyphae / Q000166:cytology* / Q000254:growth & development*; D008954:Models, Biological; D025062:Yarrowia / Q000166:cytology / Q000254:growth & development*
D000362:Agar; D005947:Glucose