Cryopreservation of Trypanosoma evansi after DEAE-cellulose purification: Evaluation of infective parameters.
Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4 months has been described. In this study, we examined the effect of purifying trypomastigote through DEAE-cellulose chromatography before and after cryopreservation, by analyzing the pre-patent period, longevity, parasitemia, and count of viable parasites. Our results showed a three-times increase in the concentration of viable trypomastigote in DEAE-purified cryopreserved parasites as compared to non-DEAE-purified cryopreserved parasites. This indicates that DEAE-cellulose chromatography followed by cryopreservation is an effective method for the storage and preservation of T. evansi, with the advantage that the stocked parasites will be ready to use in molecular biology procedures.
Tavares
Kaio César Simano
KC
Universidade do Estado de Santa Catarina, Laboratório de Bioquímica de Hemoparasitas e Vetores - LABHEV, Avenida Luiz de Camões, n° 2090, Bairro Conta Dinheiro Lages 88520-000, SC, Brazil.
Da Silva
Aleksandro Schafer
AS
Wolkmer
Patricia
P
Monteiro
Silvia Gonzalez
SG
Miletti
Luiz Claudio
LC
D000818:Animals; D000837:Anion Exchange Resins; D002845:Chromatography / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003636:DEAE-Cellulose / Q000737:chemistry*; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D014345:Trypanosoma / Q000145:classification* / Q000502:physiology*; D014352:Trypanosomiasis / Q000469:parasitology
D000837:Anion Exchange Resins; D003636:DEAE-Cellulose