Overexpression of SERCA1a in the mdx diaphragm reduces susceptibility to contraction-induced damage.
Although the precise pathophysiological mechanism of muscle damage in dystrophin-deficient muscle remains disputed, calcium appears to be a critical mediator of the dystrophic process. Duchenne muscular dystrophy patients and mouse models of dystrophin deficiency exhibit extensive abnormalities of calcium homeostasis, which we hypothesized would be mitigated by increased expression of the sarcoplasmic reticulum calcium pump. Neonatal adeno-associated virus gene transfer of sarcoplasmic reticulum ATPase 1a to the mdx diaphragm decreased centrally located nuclei and resulted in reduced susceptibility to eccentric contraction-induced damage at 6 months of age. As the diaphragm is the mouse muscle most representative of human disease, these results provide impetus for further investigation of therapeutic strategies aimed at enhanced cytosolic calcium removal.
Morine
Kevin J
KJ
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA. kmorine@gmail.com
Sleeper
Meg M
MM
Barton
Elisabeth R
ER
Sweeney
H Lee
HL
D000818:Animals; D000831:Animals, Newborn; D000229:Dependovirus / Q000235:genetics; D003964:Diaphragm / Q000378:metabolism* / Q000503:physiopathology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009119:Muscle Contraction; D053580:Muscle Strength; D020033:Protein Isoforms / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000096:biosynthesis*; D019076:Transgenes
D020033:Protein Isoforms; D011994:Recombinant Proteins; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases