title: KIOM-79 prevents xylose-induced lens opacity and inhibits TGF-beta2 in human lens epithelial cells cultured under high glucose.
abstract: OBJECTIVE
  To investigate the effects of KIOM-79 in preventing the development of diabetic complications, such as cataracts.
  
  
  METHODS
  The inhibitory effects of KIOM-79 were assessed in a model of xylose-induced lens opacity and on changes mediated by high levels of glucose in human lens epithelial (HLE-B3) cells.
  
  
  RESULTS
  In lenses treated with KIOM-79, opacity was significantly improved and glutathione (GSH) was increased compared to controls. In HLE-B3 cells treated with KIOM-79, high glucose-mediated increases in TGF-beta2, alphaB-crystallin, and fibronectin were significantly inhibited in a dose-dependent manner. KIOM-79 decreased the phosphorylation of p-Smad2/3, pp38MAPK, pp44/42, and NF-kappaB signaling in cells grown under high glucose conditions.
  
  
  CONCLUSIONS
  KIOM-79 is protective against lens opacity and protects HLE-B3 cells from the toxic effects of high glucose. Therefore, KIOM-79 may provide a potential therapeutic approach for preventing diabetic complications, such as cataracts.
authors.affiliation: Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Nan Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Jung
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D002460:Cell Line; D003318:Corneal Opacity / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D053781:Transforming Growth Factor beta2 / Q000037:antagonists & inhibitors*; D014994:Xylose / Q000633:toxicity
chemical_list: C517978:KIOM 79; D010936:Plant Extracts; D020011:Protective Agents; D053781:Transforming Growth Factor beta2; D014994:Xylose; D005978:Glutathione; D005947:Glucose