[Detection of Salmonella in water by using MUCAP test].
OBJECTIVE
To study the sensitivity, specification, detection limit and the practical use of MUCAP method for the detection of Salmonella in water.


METHODS
A comprehensive method of pre-enrichment + selective cultivation + initial screening with MUCAP + oxidase test + serological test was applied to check Salmonella in water samples spiked with bacteria and environmental water samples.


RESULTS
the sensitivity of MUCAP method was 100%, the specification was 80.8%, and the detection limit was 1 cfu/100 ml.


CONCLUSIONS
MUCAP method can effectively identify Salmonella in water and the testing time was shortening from 72h to 48h.
Zhang
Wei
W
Institute for Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Sun
Zongke
Z
Lu
Bo
B
Wang
Youbin
Y
Ding
Pei
P
Zheng
Ping
P
Chen
Xiping
X
D001431:Bacteriological Techniques; D006923:Hymecromone / Q000031:analogs & derivatives*; D007202:Indicators and Reagents; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D007202:Indicators and Reagents; C072615:4-methylumbelliferyl caprylate; D006923:Hymecromone