Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway.
Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control.
Wang
Chonglong
C
Division of Applied Life Sciences (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea.
Yoon
Sang-Hwal
SH
Shah
Asad Ali
AA
Chung
Young-Ryun
YR
Kim
Jae-Yean
JY
Choi
Eui-Sung
ES
Keasling
Jay D
JD
Kim
Seon-Won
SW
D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005204:Farnesol / Q000378:metabolism*; D018628:Gene Dosage; D015870:Gene Expression; D051228:Geranyltranstransferase / Q000235:genetics; D045782:Hemiterpenes / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008798:Mevalonic Acid / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010957:Plasmids; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism
D003470:Culture Media; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D005204:Farnesol; C004808:farnesyl pyrophosphate; D051228:Geranyltranstransferase; D008798:Mevalonic Acid