title: Detection of allogeneic blood group A and B enzyme activities in patients with ABO incompatible kidney transplantation.
abstract: The phenomenon of accommodation in recipients of blood group ABO incompatible kidney transplantation (iKTx), in which grafts survive despite the presence of blood group A or B antigen in the graft and the presence of corresponding antibodies in the recipient's blood, is not uncommon. alpha1,3-N-Acetylgalactosaminyltransferase and alpha1,3galactosyltransferase associated with the synthesis of blood group A and B antigen (A and B enzymes), respectively, were measured by a highly specific enzyme-linked immunosorbent assay in the sera and transplanted tissues of patients who underwent an ABO iKTx. Allogeneic A and B enzymes were present in the sera and tissues as well as A and B antigens in the tissues for a long period, which hitherto have never been seen in recipients prior to an iKTx. However, activities of these enzymes in the sera after an iKTx decreased in patients who experienced a serious acute antibody-mediated rejection and disappeared in patients who had an unrepairable rejection, leading to graft loss without establishment of accommodation. Our observations on the presence of allogeneic A and B enzymes in the recipients' sera should have implications in decision making for a successful iKTx.
authors.affiliation: Division of Urology, Department of Regenerative and Transplant Medicine, Niigata Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tasaki
authors.forename: Tamiko
authors.initials: T
authors.lastname: Nakajima
authors.forename: Naofumi
authors.initials: N
authors.lastname: Imai
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Saito
authors.forename: Kota
authors.initials: K
authors.lastname: Takahashi
authors.forename: Shin
authors.initials: S
authors.lastname: Yazawa
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology* / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000937:Antigen-Antibody Reactions; D001787:Blood Group Incompatibility / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005700:Galactosyltransferases / Q000378:metabolism; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D007124:Immunoenzyme Techniques; D007676:Kidney Failure, Chronic / Q000628:therapy; D016030:Kidney Transplantation / Q000276:immunology*; D008875:Middle Aged; D017350:N-Acetylgalactosaminyltransferases / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D000017:ABO Blood-Group System; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases