[The estimation of the number of platelets by different methods].
The commonly available and accurate platelet counts play an important role in the evaluation of haemorrhagic status and in assessing the need for platelet transfusions. We evaluated platelet counting performance by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometry using optical, impedance and immunological methods in trombocytopenic patients. Hematology analysers display the high precision and the accuracy of the measurement of the number of platelets in whole blood. However, the measurement of number of platelets can be disturbed by the presence of interference factors such as: microcytic erytrocytes, schizocytes, cryoglobulins, microplatelets and immunological clumps.


OBJECTIVE
The comparison of impedance, optical and immunological enumeration methods of platelets by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometer.


RESULTS
The platelet count of all considered methods showed good correlation with the cytometric method.


CONCLUSIONS
Only cytometric method with using anti-CD41 monoclonal antibody allowed to verify the results of enumeration methods of platelets.
Ratomski
Karol
K
Uniwersytet Medyczny w Bialymstoku, Zakład Laboratoryjnej Diagnostyki Pediatrycznej. karol.cam@interia.pl
Zak
Janusz
J
Kasprzycka
Edwina
E
Hryniewicz
Katarzyna
K
Wysocka
Jolanta
J
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006405:Hematology / Q000295:instrumentation / Q000379:methods; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010976:Platelet Count / Q000379:methods*; D011237:Predictive Value of Tests; D012016:Reference Values