title: Molecular diversity of arbuscular mycorrhizal fungi in relation to soil chemical properties and heavy metal contamination.
abstract: Abundance and diversity of arbuscular mycorrhizal fungi (AMF) associated with dominant plant species were studied along a transect from highly lead (Pb) and zinc (Zn) polluted to non-polluted soil at the Anguran open pit mine in Iran. Using an established primer set for AMF in the internal transcribed spacer (ITS) region of rDNA, nine different AMF sequence types were distinguished after phylogenetic analyses, showing remarkable differences in their distribution patterns along the transect. With decreasing Pb and Zn concentration, the number of AMF sequence types increased, however one sequence type was only found in the highly contaminated area. Multivariate statistical analysis revealed that further factors than HM soil concentration affect the AMF community at contaminated sites. Specifically, the soils' calcium carbonate equivalent and available P proved to be of importance, which illustrates that field studies on AMF distribution should also consider important environmental factors and their possible interactions.
authors.affiliation: Department of Soil Science, College of Agriculture, University of Shiraz, Shiraz, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Zarei
authors.forename: Stefan
authors.initials: S
authors.lastname: Hempel
authors.forename: Tesfaye
authors.initials: T
authors.lastname: Wubet
authors.forename: Tina
authors.initials: T
authors.lastname: Schäfer
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Savaghebi
authors.forename: Gholamreza Salehi
authors.initials: GS
authors.lastname: Jouzani
authors.forename: Mojtaba Khayam
authors.initials: MK
authors.lastname: Nekouei
authors.forename: François
authors.initials: F
authors.lastname: Buscot
mesh_terms: D044822:Biodiversity; D007854:Lead / Q000032:analysis* / Q000633:toxicity; D038821:Mycorrhizae / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D012987:Soil / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis* / Q000633:toxicity; D015032:Zinc / Q000032:analysis* / Q000633:toxicity
chemical_list: D012987:Soil; D012989:Soil Pollutants; D007854:Lead; D015032:Zinc