title: Non-replicating adenovirus vectors expressing avian influenza virus hemagglutinin and nucleocapsid proteins induce chicken specific effector, memory and effector memory CD8(+) T lymphocytes.
abstract: Avian influenza virus (AIV) specific CD8(+) T lymphocyte responses stimulated by intramuscular administration of an adenovirus (Ad) vector expressing either HA or NP were evaluated in chickens following ex vivo stimulation by non-professional antigen presenting cells. The CD8(+) T lymphocyte responses were AIV specific, MHC-I restricted, and cross-reacted with heterologous H7N2 AIV strain. Specific effector responses, at 10 days post-inoculation (p.i.), were undetectable at 2 weeks p.i., and memory responses were detected from 3 to 8 weeks p.i. Effector memory responses, detected 1 week following a booster inoculation, were significantly greater than the primary responses and, within 7 days, declined to undetectable levels. Inoculation of an Ad-vector expressing human NP resulted in significantly greater MHC restricted, activation of CD8(+) T cell responses specific for AIV. Decreases in all responses with time were most dramatic with maximum activation of T cells as observed following effector and effector memory responses.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Shailbala
authors.initials: S
authors.lastname: Singh
authors.forename: Haroldo
authors.initials: H
authors.lastname: Toro
authors.forename: De-Chu
authors.initials: DC
authors.lastname: Tang
authors.forename: Worthie E
authors.initials: WE
authors.lastname: Briles
authors.forename: Linda M
authors.initials: LM
authors.lastname: Yates
authors.forename: Renee T
authors.initials: RT
authors.lastname: Kopulos
authors.forename: Ellen W
authors.initials: EW
authors.lastname: Collisson
mesh_terms: D000256:Adenoviridae; D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006388:Hemagglutinins / Q000378:metabolism*; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D007371:Interferon-gamma / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
chemical_list: D006388:Hemagglutinins; D019590:Nucleocapsid Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma