Adaptation of an enzymatic polyfructosan assay to clinical practice.
Inulin or polyfructosan clearance is regarded as the most accurate method of assessing the glomerular filtration rate. We propose an enzymatic method of polyfructosan determination based on the hydrolysis of polyfructosan into fructose by inulinase and the elimination of the interfering quantity of glucose by glucose oxidase. This spectrophotometric microplate formatted assay, which demonstrated very good specificity and reproducibility (within-run precision <1% and between-run precision <3.5%), is cheap and simple to perform and can be used by all analytical laboratories and in all clinical conditions.
Dubourg
Laurence
L
Service d'Exploration Fonctionnelle Rénale et Métabolique, Hospices Civils de Lyon, Hôpital Edouard Herriot, F-69437 Lyon cedex 03, France. dubourg@sante.univ-lyon1.fr
Hadj-Aïssa
Aoumeur
A
Ferrier
Bernard
B
D004796:Clinical Enzyme Tests / Q000379:methods*; D005630:Fructans / Q000032:analysis* / Q000097:blood / Q000652:urine; D005632:Fructose / Q000378:metabolism; D005919:Glomerular Filtration Rate; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007444:Inulin / Q000378:metabolism; D015203:Reproducibility of Results
D005630:Fructans; D005632:Fructose; C002938:polyfructosan; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase