title: Inhibition of Salmonella host cell invasion by dimethyl sulfide.
abstract: We show that dimethyl sulfoxide (DMSO) inhibits Salmonella hilA expression and that this inhibition is stronger under anaerobiosis. Because DMSO can be reduced to dimethyl sulfide (DMS) during anaerobic growth, we hypothesized that DMS was responsible for hilA inhibition. Indeed, DMS strongly inhibited the expression of hilA and multiple Salmonella pathogenicity island 1 (SPI-1)-associated genes as well as the invasion of cultured epithelial cells. Because DMSO and DMS are widespread in nature, we hypothesize that this phenomenon may contribute to environmental sensing by Salmonella.
authors.affiliation: Michael Smith Laboratories, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
authors.forename: L Caetano M
authors.initials: LC
authors.lastname: Antunes
authors.forename: Michelle M C
authors.initials: MM
authors.lastname: Buckner
authors.forename: Sigrid D
authors.initials: SD
authors.lastname: Auweter
authors.forename: Rosana B R
authors.initials: RB
authors.lastname: Ferreira
authors.forename: Petra
authors.initials: P
authors.lastname: Lolić
authors.forename: B Brett
authors.initials: BB
authors.lastname: Finlay
mesh_terms: D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D004847:Epithelial Cells / Q000382:microbiology*; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D012475:Salmonella / Q000187:drug effects* / Q000502:physiology; D013440:Sulfides / Q000494:pharmacology*; D015534:Trans-Activators / Q000037:antagonists & inhibitors*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; C419942:Spi1 protein, Salmonella; D013440:Sulfides; D015534:Trans-Activators; C004784:dimethyl sulfide