Monoclonal antibodies against human Tudor-SN.
Tudor-SN is a multifunctional regulator of gene expression that has been shown to function as a transcriptional co-activator, regulator of miRNA processing, mRNA splicing, and stability. Tudor-SN has also been identified as a component in RNA-induced silencing complex. Here we have produced and characterized seven monoclonal antibody (MAb) clones against human Tudor-SN. Antibodies were generated against the fourth staphylococcal nuclease-like domain (SN4) and the Tudor domain of human Tudor-SN. The MAbs recognize the Tudor-SN protein in Western blot analysis and immunoprecipitation, and detect the specific antigen in immunohistochemistry assays. One of the antibody clones also recognizes the Drosophila melanogaster and Danio rerio Tudor-SN. Immunocytochemistry of HeLa cells revealed Tudor-SN localization in nucleolus, suggesting a possible new function for the protein in the compartment. An extensive expression analysis in human tissue arrays shows moderate to high expression of Tudor-SN in a wide range of organs and tissues, especially in epithelial cell types.
Saarikettu
Juha
J
Institute of Medical Technology, University of Tampere, Tampere, Finland.
Ovod
Vladimir
V
Vuoksio
Milka
M
Grönholm
Juha
J
Yang
Jie
J
Silvennoinen
Olli
O
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002467:Cell Nucleus / Q000276:immunology*; D004331:Drosophila melanogaster; D004720:Endonucleases; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000276:immunology*; D046888:Tissue Array Analysis; D015027:Zebrafish
D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins; D004720:Endonucleases; C095221:SND1 protein, human