Comparison of serum versus plasma collection in gas chromatography--mass spectrometry-based metabolomics.
Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco ChromaTOF software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA- versus EDTA-ASA-plasma, EDTA-plasma versus serum and EDTA-ASA-plasma versus serum, respectively. To confirm that the observed signal intensities in the GC-TOF-MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC-MS using stable-isotope-labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA-plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.
Dettmer
Katja
K
Institute of Functional Genomics, University of Regensburg, Regensburg, Germany.
Almstetter
Martin F
MF
Appel
Inka J
IJ
Nürnberger
Nadine
N
Schlamberger
Gregor
G
Gronwald
Wolfram
W
Meyer
Heinrich H D
HH
Oefner
Peter J
PJ
D000818:Animals; D002417:Cattle; D004492:Edetic Acid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010949:Plasma / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*
D004492:Edetic Acid