title: Expression of membrane-bound mucins in human nasal mucosa: different patterns for MUC4 and MUC16.
abstract: OBJECTIVE
  To acquire basic information concerning the function of the membrane-bound mucin MUC16 in nasal mucosa compared with the best-characterized membrane-bound mucin, MUC4.
  
  
  METHODS
  In vitro study using semiquantatitive reverse transcription-polymerase chain reaction analysis and immunoassay.
  
  
  METHODS
  Yeungnam University College of Medicine.
  
  
  METHODS
  We examined the nasal polyps obtained during endoscopic sinus surgery in 10 patients, the normal ethmoid sinus mucosa obtained from 10 patients, and human nasal polyp epithelial (HNPE) cells.
  
  
  METHODS
  Gene expression of MUC4 and MUC16 in nasal polyps and normal nasal mucosa. In addition, we evaluated the effect of 4 physiologically relevant agents, including retinoic acid, interleukin 1beta, phorbol 12-myristate 13-acetate (PMA), and dexamethasone, on the expression of MUC4 and MUC16 in HNPE cells at the gene and protein levels.
  
  
  RESULTS
  In nasal polyps, MUC4 was upregulated compared with normal nasal mucosa (P = .009), whereas MUC16 expression did not differ between nasal polyps and normal nasal mucosa. Retinoic acid and interleukin 1beta increased MUC4 expression at the gene and protein level in HNPE cells, whereas MUC16 expression was not affected. Unlike retinoic acid and interleukin 1beta, PMA and dexamethasone increased MUC16 expression, whereas they had no significant effect on MUC4 expression.
  
  
  CONCLUSIONS
  Expression of MUC4 and MUC16 are regulated differently in nasal mucosa. Dexamethasone and PMA are potent mediators for the expression of MUC16 in nasal polyps.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Yeungnam University College of Medicine, 317-1, Daemyung 5-Dong, Nam-Gu, Daegu 705-717, Korea.
authors.forename: Hyun-Jae
authors.initials: HJ
authors.lastname: Woo
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Bae
authors.forename: Si-Youn
authors.initials: SY
authors.lastname: Song
authors.forename: Heung-Man
authors.initials: HM
authors.lastname: Lee
authors.forename: Yong-Dae
authors.initials: YD
authors.lastname: Kim
mesh_terms: D000328:Adult; D018394:CA-125 Antigen / Q000032:analysis*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007118:Immunoassay; D053583:Interleukin-1beta / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D055264:Mucin-4 / Q000032:analysis*; D009297:Nasal Mucosa / Q000737:chemistry*; D009298:Nasal Polyps / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D018394:CA-125 Antigen; D053583:Interleukin-1beta; C451759:MUC16 protein, human; C524658:MUC4 protein, human; D008565:Membrane Proteins; D055264:Mucin-4; D014212:Tretinoin; D003907:Dexamethasone; D013755:Tetradecanoylphorbol Acetate