Evaluation of novel alginate foams as drug delivery systems in antimicrobial photodynamic therapy (aPDT) of infected wounds--an in vitro study: studies on curcumin and curcuminoides XL.
The aim of the present study was to incorporate a model water-insoluble photosensitizer, curcumin, in novel alginate foams, further to evaluate the suitability of the curcumin loaded foams in antimicrobial photodynamic therapy of infected wounds. Six foam formulations were prepared and characterized with respect to physical characteristics, in vitro release and storage- and photo-stability of curcumin. One formulation was sterilized (gamma-sterilization). The foams contained hydroxypropyl-beta-cyclodextrins or hydroxypropyl-gamma-cyclodextrins as solubilizers of curcumin. A reference foam without cyclodextrins was prepared with PEG 400 as the solubilizer. At a curcumin load of 0.153% (w/w), the water insoluble photosensitizer was uniformly distributed in the hydrophilic foams matrix. All foams were easy to handle, flexible and hydrated rapidly in a model physiological fluid. Release of curcumin in its monomeric form was demonstrated in vitro and found to be dependent on the type and amount of cyclodextrins in the formulation. Curcumin was stable during storage, but susceptible to photodegradation in the foams, especially when the formulations contain PEG 400 or hydroxypropyl-gamma-cyclodextrins. Curcumin did not degrade after gamma-sterilization, however a decrease in the in vitro release rate of curcumin and changes in the foams physical characteristics were detected.
Hegge
Anne Bee
AB
School of Pharmacy, University of Oslo, Oslo, Norway. a.b.hegge@farmasi.uio.no
Andersen
T
T
Melvik
J E
JE
Kristensen
S
S
Tønnesen
H H
HH
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000042:Absorption; D000464:Alginates / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D003116:Color; D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry*; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004355:Drug Stability; D005079:Excipients; D007700:Kinetics; D008027:Light; D010778:Photochemotherapy / Q000379:methods*; D013242:Sterilization; D014946:Wound Infection / Q000188:drug therapy*; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D000464:Alginates; D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D005079:Excipients; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C479659:hydroxypropyl-gamma-cyclodextrin; D000073738:2-Hydroxypropyl-beta-cyclodextrin; D003474:Curcumin