title: Lethal ccdB gene-based zero-background vector for construction of shotgun libraries.
abstract: A lethal ccdB gene-based vector was developed for the construction of shotgun libraries in Escherichia coli. The mutated ccdB gene carries multiple cloning sites and the amber stop codon, which simultaneously allows for maintenance of the intact vector in supE(-) strains and removal of uncut and self-ligated plasmids in supE(+) strains.
authors.affiliation: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. miyazaki-kentaro@aist.go.jp
authors.forename: Kentaro
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D004926:Escherichia coli / Q000502:physiology*; D015723:Gene Library; D024861:Genetic Enhancement / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068356:CcdB protein, Plasmid F; D011994:Recombinant Proteins