title: Genotype identification of human cystic echinococcosis in Isfahan, central Iran.
abstract: Echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of Iran with a major economic and public health importance. In the current study, Echinococcus granulosus isolates were collected from hospitalized patients in Isfahan, central Iran. The genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal DNA, followed by restriction fragment length polymorphism (RFLP) with two restriction enzymes namely AluI and MspI. As expected, each isolate yielded an approximately 1-kbp DNA fragment on the electrophoresis gel. According to RFLP results for both enzymes, all isolates had an equal pattern indicating the G1 genotype. Our findings confirmed that G1 is the dominant genotype of cystic echinococcosis in human in central Iran, with predilection to different organs including liver, lung, and brain, and warrants the importance of sheep dog cycle in public health.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Eshrat Bigom
authors.initials: EB
authors.lastname: Kia
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Rahimi
authors.forename: Mitra
authors.initials: M
authors.lastname: Sharbatkhori
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.forename: Majid
authors.initials: M
authors.lastname: Fasihi Harandi
authors.forename: Hossein
authors.initials: H
authors.lastname: Mirhendi
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D019003:Deoxyribonuclease HpaII / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004443:Echinococcosis / Q000453:epidemiology* / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D019003:Deoxyribonuclease HpaII; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific