Molecular characterization and gene expression of thyrotropin-releasing hormone in Senegalese sole (Solea senegalensis).
We describe the cloning of a cDNA encoding TRH in Solea senegalensis. Phylogenetic analysis clustered it with other fish counterparts. Eight conserved TRH progenitors and highly divergent spacers were identified. Expression profiles of TRH were analyzed in juvenile tissues, during larval development, and in post-larvae grew under four photoperiods (12L:12D, 12D:12L, 24L:0D and 24D:0L) during four weeks using a real-time PCR approach. In juvenile fish, TRH mRNA was expressed ubiquitously although with the highest transcript levels in brain. During larval development, TRH mRNA levels were higher at 2-3days after hatching decreasing progressively until metamorphosis. Hormonal treatments using thiourea and T4 showed no regulation at transcriptional level by thyroid hormones. Moreover, no significant changes in mRNA levels between photoperiods at any time were detected. Overall, these results indicate that TRH do not participate in the regulation of HPT nor adaptation to photoperiod in sole.
Iziga
Roger
R
IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro de pichón s/n, 11500 El Puerto de Santa María, Cádiz Spain.
Ponce
Marian
M
Infante
Carlos
C
Rebordinos
Laureana
L
Cañavate
Jose P
JP
Manchado
Manuel
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005412:Flatfishes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D007814:Larva / Q000378:metabolism; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D017440:Photoperiod; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D013973:Thyrotropin-Releasing Hormone