[Study on HPLC-FPS of raw and processed fructus polygoni orientalis].
OBJECTIVE
To establish the HPLC fingerprint method of Fructus Polygoni Orientalis before and after processed by choosing taxifolin as reference to compare the changes of chemical composition.


METHODS
HPLC method was emplyed with a gradient elution phase in a flow rate of 1 mL x min(-1) and the detection wavelength of 270 nm.


RESULTS
Twenty marker peaks were marked out in the raw samples and 33 marker peaks in the processed product. Methodology met was consistent with the requirement, similarity was exceeded 0.9.


CONCLUSIONS
This method is stationary, precise and feasible, which provide references of quality control for Fructus Polygoni Orientalis.
Zhai
Yanjun
Y
Liaoning University of Traditional Chinese Medicine College of Pharmacy, Dalian 116600, China. lnzyzyj@sohu.com
Zhao
Min
M
Zhang
Hui
H
Chu
Zhengyun
Z
Kang
Tingguo
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011794:Quercetin; C003377:taxifolin