title: Multiple Rad5 activities mediate sister chromatid recombination to bypass DNA damage at stalled replication forks.
abstract: DNA damage that blocks replication is bypassed in order to complete chromosome duplication and preserve cell viability and genome stability. Rad5, a PCNA polyubiquitin ligase and DNA-dependent ATPase in yeast, is orthologous to putative tumor suppressors and controls error-free damage bypass by an unknown mechanism. To identify the mechanism in vivo, we investigated the roles of Rad5 and analyzed the DNA structures that form during damage bypass at site-specific stalled forks present at replication origins. Rad5 mediated the formation of recombination-dependent, X-shaped DNA structures containing Holliday junctions between sister chromatids. Mutants lacking these damage-induced chromatid junctions were defective in resolving stalled forks, restarting replication, and completing chromosome duplication. Rad5 polyubiquitin ligase and ATPase domains both contributed to replication fork recombination. Our results indicate that multiple activities of Rad5 function coordinately with homologous recombination factors to enable replication template switch events that join sister chromatids at stalled forks and bypass DNA damage.
authors.affiliation: Department of Cancer Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Eugen C
authors.initials: EC
authors.lastname: Minca
authors.forename: David
authors.initials: D
authors.lastname: Kowalski
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D001572:Benzofurans; D002842:Chromatids / Q000235:genetics / Q000378:metabolism; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology; D053138:Cyclohexenes / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D045566:DNA, Cruciform / Q000235:genetics / Q000378:metabolism; D000080890:Duocarmycins; D007211:Indoles / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D001572:Benzofurans; D003509:Cyclohexanecarboxylic Acids; D053138:Cyclohexenes; D045566:DNA, Cruciform; D000080890:Duocarmycins; D007211:Indoles; D029701:Saccharomyces cerevisiae Proteins; C060090:adozelesin; D004247:DNA; C076225:RAD5 protein, S cerevisiae; D004265:DNA Helicases