Gold nanoparticles attenuate LPS-induced NO production through the inhibition of NF-kappaB and IFN-beta/STAT1 pathways in RAW264.7 cells.
Macrophage-derived nitric oxide (NO) plays an important role in protection against microbial infection in immune responses. Overproduction of NO by inducible nitric synthase (iNOS) is known to be closely correlated with the pathology of a variety of diseases and inflammations. In this study, we investigated the inhibitory effect of polyethylene glycol coated gold nanoparticles (GNP) on NO production and its molecular mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. It was found that GNP inhibited LPS-induced NO production and iNOS expression in RAW264.7 cells. Furthermore, GNP suppressed LPS-induced activation of NF-kappaB through the inhibition of Akt activity. GNP also inhibited LPS-induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) via down-regulation of interferon-beta (IFN-beta) expression. Our results suggest that GNP inhibits NO production and iNOS expression through blocking the activation of NF-kappaB and STAT1 in LPS-stimulated RAW264.7 cells.
Ma
Ji Su
JS
Bio-Food and Drug Research Center and Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju 380-701, South Korea.
Kim
Wan Jae
WJ
Kim
Jae Jin
JJ
Kim
Tack Joong
TJ
Ye
Sang Kyu
SK
Song
Min Dong
MD
Kang
Hyun
H
Kim
Dong Woo
DW
Moon
Won Kook
WK
Lee
Kwang Ho
KH
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D016899:Interferon-beta / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050794:STAT1 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism
D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; D009569:Nitric Oxide; D011092:Polyethylene Glycols; D006046:Gold; D016899:Interferon-beta; D052247:Nitric Oxide Synthase Type II