Naturally occurring mutations in the canine CFTR gene.
Naturally occurring cystic fibrosis (CF)-causing mutations in the CFTR gene have not been identified in any nonhuman animal species. Since domestic dogs are known to develop medical conditions associated with atypical CF in humans (e.g., bronchiectasis and pancreatitis), we hypothesized that dogs with these disorders likely have a higher expression rate of CFTR mutations than the at-large population. Temporal temperature-gradient gel electrophoresis (TTGE) was used to screen canine CFTR in 400 animals: 203 dogs diagnosed with pancreatitis, 23 dogs diagnosed with bronchiectasis, and 174 dogs admitted to clinics for any illness (at-large dogs). Twenty-eight dogs were identified with one of four CFTR missense mutations. P1281T and P1464H mutations occur in relatively unconserved residues. R1456W is analogous to the human R1453W mutation, which has approximately 20% of normal CFTR function and is associated with pancreatitis and panbronchiolitis. R812W disrupts a highly conserved protein kinase A recognition site within the regulatory domain. We conclude that naturally occurring CFTR mutations are relatively common in domestic dogs and can be detected with TTGE. No substantive differences in mutation frequency were observed between the at-large, pancreatitis, and bronchiectasis dogs.
Spadafora
Domenico
D
Department of Physiology, College of Medicine, University of South Alabama, Mobile, Alabama 36688, USA.
Hawkins
Eleanor C
EC
Murphy
Keith E
KE
Clark
Leigh Anne
LA
Ballard
Stephen T
ST
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001987:Bronchiectasis / Q000235:genetics / Q000662:veterinary; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D004252:DNA Mutational Analysis; D004283:Dog Diseases / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D005787:Gene Frequency; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D019005:Cystic Fibrosis Transmembrane Conductance Regulator