Spectroscopic studies of carotenoid-to-bacteriochlorophyll energy transfer in LHRC photosynthetic complex from Roseiflexus castenholzii.
Carotenoids present in the photosynthetic light-harvesting reaction center (LHRC) complex from chlorosome lacking filamentous anoxygenic phototroph, Roseiflexus castenholzii were purified and characterized for their photochemical properties. The LHRC from anaerobically grown cells contains five different carotenoids, methoxy-keto-myxocoxanthin, gamma-carotene, and its three derivatives, whereas the LHRC from aerobically grown cells contains only three carotenoid pigments with methoxy-keto-myxocoxanthin being the dominant one. The spectroscopic properties and dynamics of excited singlet states of the carotenoids were studied by steady-state absorption, fluorescence and ultrafast time-resolved optical spectroscopy in organic solvent and in the intact LHRC complex. Time-resolved transient absorption spectroscopy performed in the near-infrared (NIR) on purified carotenoids combined with steady-state absorption spectroscopy led to the precise determination of values of the energies of the S(1)(2(1)A(g)(-)) excited state. Global and single wavelength fitting of the ultrafast spectral and temporal data sets of the carotenoids in solvents and in the LHRC revealed the pathways of de-excitation of the carotenoid excited states.
Niedzwiedzki
Dariusz M
DM
Department of Biology, Washington University in St Louis, Missouri 63130, USA.
Collins
Aaron M
AM
LaFountain
Amy M
AM
Enriquez
Miriam M
MM
Frank
Harry A
HA
Blankenship
Robert E
RE
D000042:Absorption; D000332:Aerobiosis; D000693:Anaerobiosis; D001429:Bacteriochlorophylls / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D049109:Cell Proliferation; D041862:Chloroflexi / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D004735:Energy Transfer; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D013057:Spectrum Analysis
D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D002338:Carotenoids