title: [Immunohistochemical expression of P53 and Ki-67 in diethylnitrosamine-induced carcinomas esophageal: experimental model].
abstract: OBJECTIVE
  To evaluate the expression of P53 and Ki-67 during esophageal diethylnitrosamine (DEN)-induced carcinogenesis in 100 mice by immunohistochemistry.
  
  
  METHODS
  The animals were assigned to 4 groups, receiving water and food ad libitum. Control groups I and II received weekly esophageal gavage with cold (room temperature) or hot (60-70 degrees C) water, respectively. Experimental groups III and IV were treated with DEN for 3 consecutive days during the week, and one weekly gavage as above. The mice were sacrificed in different periods from day 30 to day 150 after the beginning of the experiment, for collection of esophageal samples which were then submitted to microscopic and immunohistochemical analyses. The temperature of the water administered by gavage was not related to the frequency of esophageal tumors.
  
  
  RESULTS
  The expression of Ki-67 was significantly higher in high-grade intraepithelial lesion (I.L.), and the expression of P53 was also higher in low-grade I.L.
  
  
  CONCLUSIONS
  The results emphasize the direct relationship of the carcinogenic process with early cell alterations detected by immunohistochemistry.
authors.affiliation: FURG, BR.
authors.forename: Miguel Angelo Martins
authors.initials: MA
authors.lastname: de Castro Junior
authors.forename: Cleber Dario Pinto
authors.initials: CD
authors.lastname: Kruel
authors.forename: Luise
authors.initials: L
authors.lastname: Meurer
authors.forename: Angela Potter
authors.initials: AP
authors.lastname: de Castro
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000276:immunology*; D004052:Diethylnitrosamine / Q000008:administration & dosage; D004938:Esophageal Neoplasms / Q000139:chemically induced / Q000276:immunology*; D005260:Female; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D051379:Mice; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D004052:Diethylnitrosamine