title: Ribosylurea accumulates in yeast urc4 mutants.
abstract: Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.
authors.affiliation: Department of Cell and Organism Biology, Lund University, Lund, Sweden.
authors.forename: O
authors.initials: O
authors.lastname: Björnberg
authors.forename: M
authors.initials: M
authors.lastname: Vodnala
authors.forename: V
authors.initials: V
authors.lastname: Domkin
authors.forename: A
authors.initials: A
authors.lastname: Hofer
authors.forename: A
authors.initials: A
authors.lastname: Rasmussen
authors.forename: G
authors.initials: G
authors.lastname: Andersen
authors.forename: J
authors.initials: J
authors.lastname: Piskur
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012266:Ribose / Q000737:chemistry / Q000378:metabolism; D012440:Saccharomyces / Q000235:genetics* / Q000378:metabolism*; D014498:Uracil / Q000737:chemistry / Q000378:metabolism*; D014508:Urea / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014498:Uracil; D012266:Ribose; D014508:Urea