Comprehensive maternal serum proteomic profiles of preclinical and clinical preeclampsia.
We systematically characterized maternal serum proteome in women with clinical preeclampsia (PE) and asymptomatic women in early pregnancy that subsequently developed PE. Clinical PE cohort comprised 30 patients with mild PE, 30 with severe PE, and 58 normotensive women. Preclinical PE cohort included 149 women whose serum samples were collected at 8-14 gestational weeks and in whom 30 women later developed mild and 40 severe PE. Serum proteome was analyzed and enzyme-linked immunosorbent assays were used for protein quantification. In Clinical PE, fibronectin, pappalysin-2, choriogonadotropin-beta, apolipoprotein C-III, cystatin-C, vascular endothelial growth factor receptor-1, and endoglin were more abundant compared to normotensive women. In preclinical PE, differently expressed proteins included placental, vascular, transport, matrix, and acute phase proteins. Angiogenic and antiangiogenic proteins were not significant. We conclude that placental and antiangiogenic proteins are abundant in clinical PE. In preclinical PE, proteomic profile is distinct and different from that in clinical PE.
Rasanen
Juha
J
Departments of Obstetrics and Gynecology, Pediatrics, Public Health and Preventive Medicine, Oregon Health & Science University, Portland, Oregon 97239, USA. juha.rasanen@oulu.fi
Girsen
Anna
A
Lu
Xinfang
X
Lapidus
Jodi A
JA
Standley
Melissa
M
Reddy
Ashok
A
Dasari
Surendra
S
Thomas
Archana
A
Jacob
Thomas
T
Pouta
Anneli
A
Surcel
Helja-Marja
HM
Tolosa
Jorge E
JE
Gravett
Michael G
MG
Nagalla
Srinivasa R
SR
D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016015:Logistic Models; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D001798:Blood Proteins