title: Protective effect of rebamipide against Helicobacter pylori-CagA-induced effects on gastric epithelial cells.
abstract: BACKGROUND
  Helicobacter pylori CagA dysregulates cell signaling pathways and leads to targeted transcriptional up-regulation of genes implicated in gastric cell injury. The aim of this study was to determine the effects of rebamipide on CagA-induced effects on gastric epithelial cells. We investigated the effects of rebamipide treatment (pre- or post-treatment before or after CagA transfection) on CagA-induced gastric cell injury.
  
  
  METHODS
  We evaluated the morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization by confocal microscopy, IL-8 production by ELISA, and NF-κB activation by luciferase assay in AGS gastric epithelial cells and MDCK cells.
  
  
  RESULTS
  Transfection of CagA into gastric epithelial cells induced morphologic changes (hummingbird phenotype), ZO-1 mislocalization, and IL-8 production in gastric epithelial cells. Pre-treatment with rebamipide inhibits CagA-induced effects on gastric epithelial cells, including morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization, IL-8 production, and NF-κB activity.
  
  
  CONCLUSIONS
  These results suggest that rebamipide might have a potential role in the protection of H. pylori CagA-induced effects on gastric epithelial cells.
authors.affiliation: Department of Pathology, Pusan National University School of Medicine and Pusan National University Hospital, Pusan National University, Seo-Gu, Busan, Republic of Korea.
authors.forename: Kyung Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Jee-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Woo-Kyung
authors.initials: WK
authors.lastname: Kim
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Shin
authors.forename: Kyung-Un
authors.initials: KU
authors.lastname: Choi
authors.forename: Dae Whan
authors.initials: DW
authors.lastname: Kim
authors.forename: Woo-Jeong
authors.initials: WJ
authors.lastname: Lee
authors.forename: Jin-Hwa
authors.initials: JH
authors.lastname: Choi
authors.forename: Soo Han
authors.initials: SH
authors.lastname: Lee
authors.forename: Gwang Ha
authors.initials: GH
authors.lastname: Kim
authors.forename: Geun Am
authors.initials: GA
authors.lastname: Song
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Jeon
authors.forename: Chi Dae
authors.initials: CD
authors.lastname: Kim
authors.forename: Ki Whan
authors.initials: KW
authors.lastname: Hong
authors.forename: Do Youn
authors.initials: DY
authors.lastname: Park
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000633:toxicity*; D002460:Cell Line; D004285:Dogs; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D015363:Quinolones / Q000494:pharmacology*; D013270:Stomach / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D062826:Zonula Occludens-1 Protein
chemical_list: D000897:Anti-Ulcer Agents; D000942:Antigens, Bacterial; D000975:Antioxidants; D001426:Bacterial Proteins; D016209:Interleukin-8; D008565:Membrane Proteins; D016328:NF-kappa B; D010750:Phosphoproteins; D015363:Quinolones; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C080866:cagA protein, Helicobacter pylori; C052785:rebamipide; D000409:Alanine