title: Complete nucleotide sequence and genome organization of Olive latent virus 3, a new putative member of the family Tymoviridae.
abstract: The complete nucleotide sequence and the genome organization were determined of a putative new member of the family Tymoviridae, tentatively named Olive latent virus 3 (OLV-3), recovered in southern Italy from a symptomless olive tree. The sequenced ssRNA genome comprises 7148 nucleotides excluding the poly(A) tail and contains four open reading frames (ORFs). ORF1 encodes a polyprotein of 221.6kDa in size, containing the conserved signatures of the methyltransferase (MTR), papain-like protease (PRO), helicase (HEL) and RNA-dependent RNA polymerase (RdRp) domains of the replication-associated proteins of positive-strand RNA viruses. ORF2 overlaps completely ORF1 and encodes a putative protein of 43.33kDa showing limited sequence similarity with the putative movement protein of Maize rayado fino virus (MRFV). ORF3 codes for a protein with predicted molecular mass of 28.46kDa, identified as the coat protein (CP), whereas ORF4 overlaps ORF3 and encodes a putative protein of 16kDa with sequence similarity to the p16 and p31 proteins of Citrus sudden death-associated virus (CSDaV) and Grapevine fleck virus (GFkV), respectively. Within the family Tymoviridae, OLV-3 genome has the closest identity level (49-52%) with members of the genus Marafivirus, from which, however, it differs because of the diverse genome organization and the presence of a single type of CP subunits.
authors.affiliation: Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, Italy.
authors.forename: Abdulkader
authors.initials: A
authors.lastname: Alabdullah
authors.forename: Angelantonio
authors.initials: A
authors.lastname: Minafra
authors.forename: Toufic
authors.initials: T
authors.lastname: Elbeaino
authors.forename: Maria
authors.initials: M
authors.lastname: Saponari
authors.forename: Vito
authors.initials: V
authors.lastname: Savino
authors.forename: Giovanni P
authors.initials: GP
authors.lastname: Martelli
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D031658:Olea / Q000821:virology; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D046830:Tymoviridae / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins