An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.
BACKGROUND
In metazoans, the hematopoietic system plays a key role both in normal development and in defense of the organism. In Drosophila, the cellular immune response involves three types of blood cells: plasmatocytes, crystal cells and lamellocytes. This last cell type is barely present in healthy larvae, but its production is strongly induced upon wasp parasitization or in mutant contexts affecting larval blood cell homeostasis. Notably, several zygotic mutations leading to melanotic mass (or "tumor") formation in larvae have been associated to the deregulated differentiation of lamellocytes. To gain further insights into the gene regulatory network and the mechanisms controlling larval blood cell homeostasis, we conducted a tissue-specific loss of function screen using hemocyte-specific Gal4 drivers and UAS-dsRNA transgenic lines.


RESULTS
By targeting around 10% of the Drosophila genes, this in vivo RNA interference screen allowed us to recover 59 melanotic tumor suppressor genes. In line with previous studies, we show that melanotic tumor formation is associated with the precocious differentiation of stem-cell like blood progenitors in the larval hematopoietic organ (the lymph gland) and the spurious differentiation of lamellocytes. We also find that melanotic tumor formation can be elicited by defects either in the fat body, the embryo-derived hemocytes or the lymph gland. In addition, we provide a definitive confirmation that lymph gland is not the only source of lamellocytes as embryo-derived plasmatocytes can differentiate into lamellocytes either upon wasp infection or upon loss of function of the Friend of GATA cofactor U-shaped.


CONCLUSIONS
In this study, we identify 55 genes whose function had not been linked to blood cell development or function before in Drosophila. Moreover our analyses reveal an unanticipated plasticity of embryo-derived plasmatocytes, thereby shedding new light on blood cell lineage relationship, and pinpoint the Friend of GATA transcription cofactor U-shaped as a key regulator of the plasmatocyte to lamellocyte transformation.
Avet-Rochex
Amélie
A
Université de Toulouse, UPS, CBD Centre de Biologie du Développement, Bât4R3, 118 route de Narbonne, 31062 Toulouse, France.
Boyer
Karène
K
Polesello
Cédric
C
Gobert
Vanessa
V
Osman
Dani
D
Roch
Fernando
F
Augé
Benoit
B
Zanet
Jennifer
J
Haenlin
Marc
M
Waltzer
Lucas
L
D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D053263:Gene Regulatory Networks; D016147:Genes, Tumor Suppressor; D006410:Hematopoiesis; D006434:Hemocytes / Q000166:cytology / Q000276:immunology; D006706:Homeostasis; D034622:RNA Interference