Rationally improving LOV domain-based photoswitches.
Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
Strickland
Devin
D
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
Yao
Xiaolan
X
Gawlak
Grzegorz
G
Rosen
Michael K
MK
Gardner
Kevin H
KH
Sosnick
Tobin R
TR
D018554:Avena; D008027:Light; D010777:Photochemistry / Q000379:methods*; D056949:Phototropins / Q000737:chemistry* / Q000528:radiation effects; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000528:radiation effects
D056949:Phototropins; D011506:Proteins