Dendritic cells interact with CD4 T cells in intestinal mucosa.
Absence of lymph nodes in nonmammalian species, expression of MHCII by APCs in the periphery, and the recent findings that T cells can change their polarization status after presentation in the lymph nodes imply a role for MHCII-mediated presentation outside the organized lymphoid tissue. This study shows that MHCII(+) ECs and DCs from the intestinal mucosa of the pig can present antigen to T cells in vitro. In vivo, APCs colocalize with T cells in pig and mouse intestinal mucosa. In the pig, endothelium is involved in these interactions in neonates but not in adults, indicating different roles for stromal and professional APCs in the neonate compared with the adult. The ratio of expression of DQ and DR MHCII locus products was lower on ECs than on other mucosal APCs, indicating that the two types of cells present different peptide sets. Adult nonendothelial APCs expressed a higher ratio of DQ/DR than in neonates. These results suggest that mucosal DCs can present antigen locally to primed T cells and that stromal APCs are recruited to these interactions in some cases. This raises the possibility that local presentation may influence T cell responses at the effector stage after initial presentation in the lymph node.
Inman
Charlotte F
CF
Division of Veterinary Pathology, Infection and Immunity, School of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK. c.f.inman@bristol.ac.uk
Singha
Sakon
S
Lewis
Marie
M
Bradley
Ben
B
Stokes
Chris
C
Bailey
Mick
M
D000375:Aging / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D045142:Cross-Priming / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D007583:Jejunum / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D034421:Sus scrofa
D000949:Histocompatibility Antigens Class II; D007519:Isoantigens