The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells.
Nucleoporin 153 (Nup153), a component of the nuclear pore complex (NPC), has been implicated in the interaction of the NPC with the nuclear lamina. Here we show that depletion of Nup153 by RNAi results in alteration of the organization of the nuclear lamina and the nuclear lamin-binding protein Sun1. More striking, Nup153 depletion induces a dramatic cytoskeletal rearrangement that impairs cell migration in human breast carcinoma cells. Our results point to a very prominent role of Nup153 in connection to cell motility that could be exploited in order to develop novel anti-cancer therapy.
Zhou
Lixin
L
Department of Zoology, University of British Columbia, Vancouver, BC, Canada.
Panté
Nelly
N
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D022082:Cellular Structures / Q000378:metabolism; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism*; D034881:Nuclear Lamina / Q000235:genetics / Q000378:metabolism; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D034622:RNA Interference; D014982:Xenopus laevis
D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins