Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions.
OBJECTIVE
The aim of the present study was to investigate the effects of MeOH extract of PL (PLME) and its fractions on angiogenesis.


METHODS
PLME and its subsequent fractions (methylene chloride, ethyl acetate, n-butanol and aqueous fractions) were evaluated in vitro. Specifically, the anti-angiogenic activities of PLME and its fractions were investigated by measuring their effects on the proliferation, migration, tube formation and phosphorylation of vascular endothelial growth factor receptor (VEGFR)-2 in human umbilical vein endothelial cells (HUVECs). In addition, the in vivo Matrigel plug model was applied to evaluate new vessel formation.


RESULTS
The results revealed that PLME and its subsequent fractions, except for the aqueous fraction, led to significant inhibition of the proliferation, migration, tube formation and VEGFR-2 phosphorylation of HUVECs as well as in vivo angiogenesis.


CONCLUSIONS
These findings indicate the potential for the use of PLME in pathological situations involving stimulated angiogenesis, such as inflammation and tumor development.
Lee
Yeon Sil
YS
Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 39 Hallym daehakgil, Chuncheon 200-702, Republic of Korea.
Kim
Yoon Hee
YH
Shin
Eun Kyung
EK
Kim
Dae Hwan
DH
Lim
Soon Sung
SS
Lee
Jae-Yong
JY
Kim
Jin-Kyung
JK
D000363:Agaricales; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D000432:Methanol / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D000084682:Phellinus; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000037:antagonists & inhibitors / Q000378:metabolism
D020533:Angiogenesis Inhibitors; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D040301:Vascular Endothelial Growth Factor Receptor-2; D000432:Methanol