Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde.
3-Hexulose-6-phosphate synthase (HPS) and 6-phosphate-3-hexuloisomerase (PHI) are two key enzymes in the formaldehyde (HCHO) assimilation pathway in methylotrophs. The HPS/PHI fusion protein, encoded by the chimeric gene of hps and phi from Mycobacterium gastri MB19, possesses both HPS and PHI activities in an Escherichia coli transformant. Overexpression of the fusion protein in chloroplasts of geranium (Pelargonium sp. Frensham) created a photosynthetic HCHO assimilation pathway according to (13)C-NMR analysis. The transgenic plants exhibited an enhanced ability in HCHO-uptake and [(14)C]HCHO-assimilation. Moreover, the transgenic plants showed greater HCHO-resistance and stronger capacity in purification of the HCHO-polluted air. Therefore, the use of the single chimeric gene may not only greatly simplify the transformation procedure but also improve the efficiency of phytoremediating HCHO in ornamental plants.
Song
Zhongbang
Z
Biotechnology Research Center, Kunming University of Science and Technology, Bailong Campus, Bailongsi, Bailong Road, Kunming, 650224, China.
Orita
Izumi
I
Yin
Fei
F
Yurimoto
Hiroya
H
Kato
Nobuo
N
Sakai
Yasuyoshi
Y
Izui
Katsura
K
Li
Kunzhi
K
Chen
Limei
L
D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism*; D031315:Geranium / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009161:Mycobacterium / Q000201:enzymology* / Q000235:genetics; D010788:Photosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D011993:Recombinant Fusion Proteins; D005557:Formaldehyde; D000446:Aldehyde-Lyases; C021355:hexose phosphate synthetase; D019747:Aldose-Ketose Isomerases; C023918:phospho-3-hexuloisomerase