title: Enhanced volatile phenols in wine fermented with Saccharomyces cerevisiae and spoiled with Pichia guilliermondii and Dekkera bruxellensis.
abstract: OBJECTIVE
  To investigate whether the presence of Pichia guilliermondii impacts on the production of volatile phenols from mixed wine fermentations with Dekkera bruxellensis and Saccharomyces cerevisiae.
  
  
  RESULTS
  Four inoculation strategies were performed in small-scale fermentations involving P. guilliermondii, D. bruxellensis and S. cerevisiae using Syrah grape juice supplemented with 100 mg l(-1) of p-coumaric acid. High pressure liquid chromatography was used for the quantification or volatile phenols. Significant high levels of 4-ethylphenol and 4-ethylguaicol (720 and 545 microg l(-1), respectively), as well as the highest levels of 4-vinylphenol (>4500 microg l(-1)), were observed when P. guilliermondii species was inoculated from the beginning of the fermentation.
  
  
  CONCLUSIONS
  The metabolic interaction occurring between the high vinylphenol producer species P. guilliermondii and D. bruxellensis exhibiting a high vinylphenol reductase activity resulted in an increased production of volatile phenols in wine.
  
  
  CONCLUSIONS
  Pichia guilliermondii must be considered a very important spoilage yeast in the wine industry capable of producing large amounts of volatile phenols.
authors.affiliation: Laboratorio de Microbiología y Biotecnología, Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte (IDEPA, CONICET-Universidad Nacional del Comahue), Buenos Aires, Neuquén, Argentina.
authors.forename: J S
authors.initials: JS
authors.lastname: Sáez
authors.forename: C A
authors.initials: CA
authors.lastname: Lopes
authors.forename: V C
authors.initials: VC
authors.lastname: Kirs
authors.forename: M P
authors.initials: MP
authors.lastname: Sangorrín
mesh_terms: D002851:Chromatography, High Pressure Liquid; D055167:Dekkera / Q000254:growth & development / Q000378:metabolism*; D010636:Phenols / Q000032:analysis*; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000032:analysis*; D014920:Wine / Q000382:microbiology*
chemical_list: D010636:Phenols; D055549:Volatile Organic Compounds