title: Delta1 family members are involved in filopodial actin formation and neuronal cell migration independent of Notch signaling.
abstract: Delta family proteins are transmembrane molecules that bind Notch receptors and activate downstream signaling events in neighboring cells. In addition to serving as Notch ligands, Notch-independent roles for Delta have been suggested but are not fully understood. Here, we demonstrate a previously unrecognized role for Delta in filopodial actin formation. Delta1 and Delta4, but not Delta3, exhibit filopodial protrusive activity, and this activity is independent of Notch signaling. The filopodial activity of Delta1 does not depend on the PDZ-binding domain at the C-terminus; however, the intracellular membrane-proximal region that is anchored to the plasma membrane plays an important role in filopodial activity. We further identified a Notch-independent role of DeltaD in neuronal cell migration in zebrafish. These findings suggest a possible functional link between Notch-independent filopodial activity of Delta and the control of cell motility.
authors.affiliation: Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Nishide
authors.forename: Hiromi
authors.initials: H
authors.lastname: Matsuo
authors.forename: Sayumi
authors.initials: S
authors.lastname: Okigawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Okano
authors.forename: Tohru
authors.initials: T
authors.lastname: Ishitani
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002465:Cell Movement; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D011554:Pseudopodia / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D015027:Zebrafish / Q000254:growth & development
chemical_list: D000199:Actins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D051880:Receptors, Notch; C064045:delta protein