Functional analysis of human organic cation transporter OCT3 (SLC22A3) polymorphisms.
We analyzed the functional properties of five single nucleotide polymorphisms (SNPs) in organic cation transporter OCT3 gene (SLC22A3) resulting in the amino acid changes with a transient expression system. Three SNPs (A116S, T400I, and A439V) exhibited reduced uptake of both [(3)H]histamine and [(3)H]MPP(+), although their protein expressions were detected in the plasma membrane of transfected cells. This study suggests that the OCT3 variants will contribute to inter-individual variations leading to the differences in cationic drug disposition as well as certain disease processes such as hypertension, allergic diseases, and neuropsychiatric diseases by the clearance of endogenous organic cations such as biogenic amines.
Sakata
Takeshi
T
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Anzai
Naohiko
N
Kimura
Toru
T
Miura
Daisaku
D
Fukutomi
Toshiyuki
T
Takeda
Michio
M
Sakurai
Hiroyuki
H
Endou
Hitoshi
H
D015655:1-Methyl-4-phenylpyridinium / Q000378:metabolism; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics; D019556:COS Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D030541:Databases, Genetic; D005787:Gene Frequency; D056726:Genetic Association Studies; D006632:Histamine / Q000493:pharmacokinetics; D006801:Humans; D009498:Neurotoxins / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D014162:Transfection; D000099254:Organic Cation Transporter 3
D015655:1-Methyl-4-phenylpyridinium; D015306:Biogenic Monoamines; D006632:Histamine; D009498:Neurotoxins; D027701:Organic Cation Transport Proteins; D000099254:Organic Cation Transporter 3