[Expression of mast cell tryptase in scar].
OBJECTIVE
To investigate the expression and distribution of mast cell tryptase (MCT) in scar, and to discuss the different MCT gene expression in keloid, hypertrophic scar and normal skin.


METHODS
20 samples of keloid, 20 samples of hypertrophic scar and 20 samples of normal skin were collected. The distribution of MCT was investigated by immunofluorescence histochemistry, and the MCT mRNA expression was detected by Relative Quantification real-time fluorescent PCR.


RESULTS
MCT gene was mainly located in the collagen fiber bundles of the scar, especially in the superficial layer of scar. MCT mRNA expression was significantly higher in keloid than that in hypertrophic scar and normal skin (P < 0.01). Averagely, the MCT gene expression in keloid was 2.5 times and 5.4 times of that in hypertrophic scar and normal skin.


CONCLUSIONS
MCT gene may play a role in the pathogenesis of scar.
Gao
Feng
F
Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Zhao
Yang
Y
Feng
Yong-qiang
YQ
Huo
Ran
R
Xue
Wen-jun
WJ
Wang
Fa-gang
FG
Lv
Ren-rong
RR
Xue
Feng
F
Li
Qiang
Q
Zhang
Jian
J
D000293:Adolescent; D000328:Adult; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000378:metabolism / Q000473:pathology; D053802:Tryptases / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D012333:RNA, Messenger; D053802:Tryptases