title: Up-regulated microRNA-146a negatively modulate Helicobacter pylori-induced inflammatory response in human gastric epithelial cells.
abstract: Helicobacter pylori (H. pylori) is a major human pathogenic bacterium in gastric mucosa. However, the regulatory mechanism of H. pylori-induced immune response is not clear. MicroRNAs (miRNAs) have recently emerged as key post-transcriptional regulators of gene expression, and their role in H. pylori infection is just beginning to be explored. Here, we first reported that H. pylori infection up-regulated the expression of miR-146a in gastric epithelial cells as well as in gastric mucosal tissues in NF-κB-dependent manner. In turn, miR-146a may downregulate the expression of target genes, interleukin-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6). Furthermore, miR-146a negatively regulated H. pylori-triggered interleukin (IL)-8, growth-related oncogene (GRO)-α, and macrophage inflammatory protein (MIP) -3α through diminishing NF-κB activity. In conclusion, H. pylori-induced miR-146a plays a potential role in a negative feedback loop to modulate the inflammation by targeting IRAK1 and TRAF6.
authors.affiliation: Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.forename: Bosheng
authors.initials: B
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Endong
authors.initials: E
authors.lastname: Zhu
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liu
authors.forename: Honglei
authors.initials: H
authors.lastname: Ding
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
mesh_terms: D000328:Adult; D000368:Aged; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D005786:Gene Expression Regulation; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007249:Inflammation; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D016328:NF-kappa B / Q000096:biosynthesis; D048029:TNF Receptor-Associated Factor 6 / Q000096:biosynthesis*
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D048029:TNF Receptor-Associated Factor 6; D053592:Interleukin-1 Receptor-Associated Kinases