title: Mechanistic investigations into interactions between IGF-I and IGFBPs and their impact on facilitating cell migration on vitronectin.
abstract: Numerous studies have reported links between insulin-like growth factors (IGFs) and the extra-cellular matrix protein vitronectin (VN). We ourselves have reported that IGF-I binds to VN via IGF-binding proteins (IGFBPs) to stimulate HaCaT and MCF-7 cell migration. Here, we detail the functional evaluation of IGFBP-1, -2, -3, -4 and -6 in the presence and absence of IGF-I and VN. The data presented here, combined with our prior data on IGFBP-5, suggest that IGFBP-3, -4 and -5 are the most effective at stimulating cell migration in combination with IGF-I and VN. In addition, we demonstrate that different regions within IGFBP-3 and -4 are critical for complex formation. Furthermore, we examine whether multi-protein complexes of IGF-I and IGFBPs associated with fibronectin and collagen IV are also able to enhance functional biological responses.
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, 4059, Queensland, Australia.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Kricker
authors.forename: Carolyn E
authors.initials: CE
authors.lastname: Hyde
authors.forename: Derek R
authors.initials: DR
authors.lastname: Van Lonkhuyzen
authors.forename: Brett G
authors.initials: BG
authors.lastname: Hollier
authors.forename: Gary K
authors.initials: GK
authors.lastname: Shooter
authors.forename: David I
authors.initials: DI
authors.lastname: Leavesley
authors.forename: Adrian C
authors.initials: AC
authors.lastname: Herington
authors.forename: Zee
authors.initials: Z
authors.lastname: Upton
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D024141:Collagen Type IV / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000494:pharmacology*; D054730:Protein Interaction Domains and Motifs; D019096:Vitronectin / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; D018969:Insulin-Like Growth Factor Binding Proteins; D019096:Vitronectin; D007334:Insulin-Like Growth Factor I