Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering.
Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production.
Marui
Junichiro
J
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Higashi 1-1-1, AIST Tsukuba Central 6, Tsukuba 305-8566, Ibaraki, Japan.
Ohashi-Kunihiro
Sumiko
S
Ando
Tomohiro
T
Nishimura
Marie
M
Koike
Hideaki
H
Machida
Masayuki
M
D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D005818:Genetic Engineering; D010406:Penicillins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics
D010406:Penicillins; D011994:Recombinant Proteins