title: Covering all bases in HIV research: unveiling a hidden world of viral evolution.
abstract: The advent of next-generation sequencing technologies has greatly impacted genomic research by providing a means for increasing the amount of sequence information in a cost effective fashion. Among the major next-generation sequencing technologies, the Roche 454 platform has been widely adopted in HIV research. We discuss a broad range of applications of the 454 pyrosequencing platform in the HIV field, with a particular emphasis on antiretroviral therapy and virus-host interaction-related research. We also highlight some of the bioinformatics challenges, as well as advantages and potential limitations of this "deep" sequencing tool, and hint at future research applications.
authors.affiliation: Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium. bram.vrancken@rega.kuleuven.be
authors.forename: Bram
authors.initials: B
authors.lastname: Vrancken
authors.forename: Sébastian
authors.initials: S
authors.lastname: Lequime
authors.forename: Kristof
authors.initials: K
authors.lastname: Theys
authors.forename: Philippe
authors.initials: P
authors.lastname: Lemey
mesh_terms: D019380:Anti-HIV Agents / Q000627:therapeutic use; D019143:Evolution, Molecular; D006678:HIV / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral