title: Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
abstract: L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
authors.affiliation: Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lee
authors.forename: Aine
authors.initials: A
authors.lastname: Fox
authors.forename: Janice
authors.initials: J
authors.lastname: Sufrin
authors.forename: Hugues
authors.initials: H
authors.lastname: Henry
authors.forename: Paul
authors.initials: P
authors.lastname: Majcherczyk
authors.forename: Dieter
authors.initials: D
authors.lastname: Haas
authors.forename: Cornelia
authors.initials: C
authors.lastname: Reimmann
mesh_terms: D000613:Aminobutyrates / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000963:Antimetabolites / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D023061:Gene Order; D005798:Genes, Bacterial; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000613:Aminobutyrates; D000900:Anti-Bacterial Agents; D000963:Antimetabolites; C000264:2-amino-4-methoxy-3-butenoic acid