Bacterial growth monitoring in a microfluidic device by confocal reflection microscopy.
The feasibility of a method to nondestructively measure planktonic bacterial growth in a microfluidic device was addressed. Here, we report that the growth of Pseudomonas aeruginosa in a microfluidic device could be measured by a three-dimensional image analysis technique based on confocal reflection microscopy in a time-course.
Yawata
Yutaka
Y
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennnoudai1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
Toda
Kensuke
K
Setoyama
Erika
E
Fukuda
Junji
J
Suzuki
Hiroaki
H
Uchiyama
Hiroo
H
Nomura
Nobuhiko
N
D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA; D021621:Imaging, Three-Dimensional / Q000379:methods; D046210:Microfluidic Analytical Techniques; D018613:Microscopy, Confocal; D011550:Pseudomonas aeruginosa / Q000254:growth & development*
D004247:DNA