title: rQuant.web: a tool for RNA-Seq-based transcript quantitation.
abstract: We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tackle multiple transcripts per gene locus and is therefore particularly well suited to quantify alternative transcripts. rQuant.web is available as a tool in a Galaxy installation at http://galaxy.fml.mpg.de. Using rQuant.web is free of charge, it is open to all users, and there is no login requirement.
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany. regina.bohnert@tuebingen.mpg.de
authors.forename: Regina
authors.initials: R
authors.lastname: Bohnert
authors.forename: Gunnar
authors.initials: G
authors.lastname: Rätsch
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D020407:Internet; D012333:RNA, Messenger / Q000032:analysis; D017423:Sequence Analysis, RNA; D012984:Software
chemical_list: D012333:RNA, Messenger