Neuronal growth-inhibitory factor (metallothionein-3): structure-function relationships.
Neuronal growth-inhibitory factor (GIF), also named metallothionein-3, inhibits the outgrowth of neuronal cells. Recent studies on the structure of human GIF, carried out using NMR and molecular dynamics simulation techniques, have been summarized. By studying a series of protein-engineered mutants of GIF, we showed that the bioactivity of GIF is modulated by multiple factors, including the unique TCPCP motif-induced characteristic conformation, the solvent accessibility and dynamics of the metal-thiolate cluster, and the domain-domain interactions.
Ding
Zhi-Chun
ZC
Chemical Biology Laboratory, Department of Chemistry, Fudan University, Shanghai, China.
Ni
Feng-Yun
FY
Huang
Zhong-Xian
ZX
D000818:Animals; D006801:Humans; D000090023:Metallothionein 3; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D015202:Protein Engineering; D013329:Structure-Activity Relationship
D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins