title: TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa.
abstract: In adaptive immunity, Th17 lymphocytes produce the IL-17 and IL-22 cytokines that stimulate mucosal antimicrobial defenses and tissue repair. In this study, we observed that the TLR5 agonist flagellin induced swift and transient transcription of genes encoding IL-17 and IL-22 in lymphoid, gut, and lung tissues. This innate response also temporarily enhanced the expression of genes associated with the antimicrobial Th17 signature. The source of the Th17-related cytokines was identified as novel populations of CD3(neg)CD127(+) immune cells among which CD4-expressing cells resembling lymphoid tissue inducer cells. We also demonstrated that dendritic cells are essential for expression of Th17-related cytokines and so for stimulation of innate cells. These data define that TLR-induced activation of CD3(neg)CD127(+) cells and production of Th17-related cytokines may be crucial for the early defenses against pathogen invasion of host tissues.
authors.affiliation: Institut Pasteur de Lille, Centre d'Infection et d'Immunité de Lille, France.
authors.forename: Laurye
authors.initials: L
authors.lastname: Van Maele
authors.forename: Christophe
authors.initials: C
authors.lastname: Carnoy
authors.forename: Delphine
authors.initials: D
authors.lastname: Cayet
authors.forename: Pascal
authors.initials: P
authors.lastname: Songhet
authors.forename: Laure
authors.initials: L
authors.lastname: Dumoutier
authors.forename: Isabel
authors.initials: I
authors.lastname: Ferrero
authors.forename: Laure
authors.initials: L
authors.lastname: Janot
authors.forename: François
authors.initials: F
authors.lastname: Erard
authors.forename: Julie
authors.initials: J
authors.lastname: Bertout
authors.forename: Hélène
authors.initials: H
authors.lastname: Leger
authors.forename: Florent
authors.initials: F
authors.lastname: Sebbane
authors.forename: Arndt
authors.initials: A
authors.lastname: Benecke
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Renauld
authors.forename: Wolf-Dietrich
authors.initials: WD
authors.lastname: Hardt
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ryffel
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Sirard
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005260:Female; D005408:Flagellin / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D007082:Ileum / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D017252:CD3 Complex; D005408:Flagellin; D020381:Interleukin-17; D053699:Interleukin-7 Receptor alpha Subunit; D007378:Interleukins; D051198:Toll-Like Receptor 5; D000097043:Interleukin-22