[Homology analysis of Vibrio parahaemolyticus isolated by MPN method using pulsed-field gel electrophoresis and automated ribotyping].
OBJECTIVE
To study the molecular typing of 78 strains of Vibrio parahaemolyticus isolated from Guangdong by MPN method with pulsed-field gel electrophoresis (PFGE) and automated ribotyping (RP).


METHODS
DNAs from all strains were digested by EcoR I for ribotyping and digested by Sfi I and Not I for PFGE. The band patterns of PFGE and RP were clustering adn analyzed by BioNumerics software.


RESULTS
There were 64, 68 and 71 kinds of band patterns of each Vibrio parahaemolyticus strain digested by restriction endonuclease EcoR I, Sfi I and Not I respectively. The band patterns of some strains from the same sample are completely identical, but the band patterns of most strains are different and are divided into different groups.


CONCLUSIONS
PFGE is a rapid, robust, and reproducible genotyping technique, more discriminative than automated ribotyping. Choosing more isolates from MPN method for suspected food samples should be considered in tracing the phylogenetic relationship of strains.
Li
Weiwei
W
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. liww_1984@hotmail.com
Wang
Xiaoying
X
Pei
Xiaoyan
X
Zhu
Haiming
H
Guo
Yunchang
Y
D015373:Bacterial Typing Techniques / Q000379:methods*; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005516:Food Microbiology; D021521:Ribotyping / Q000379:methods*; D017385:Sequence Homology; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000302:isolation & purification*
D004262:DNA Restriction Enzymes