title: Altered manganese homeostasis and manganese toxicity in a Huntington's disease striatal cell model are not explained by defects in the iron transport system.
abstract: Expansion of a polyglutamine tract in Huntingtin (Htt) leads to the degeneration of medium spiny neurons in Huntington's disease (HD). Furthermore, the HTT gene has been functionally linked to iron (Fe) metabolism, and HD patients show alterations in brain and peripheral Fe homeostasis. Recently, we discovered that expression of mutant HTT is associated with impaired manganese (Mn) uptake following overexposure in a striatal neuronal cell line and mouse model of HD. Here we test the hypothesis that the transferrin receptor (TfR)-mediated Fe uptake pathway is responsible for the HD-associated defects in Mn uptake. Western blot analysis showed that TfR levels are reduced in the mutant STHdh(Q111/Q111) striatal cell line, whereas levels of the Fe and Mn transporter, divalent metal transporter 1 (DMT1), are unchanged. To stress the Fe transport system, we exposed mutant and wild-type cells to elevated Fe(III), which revealed a subtle impairment in net Fe uptake only at the highest Fe exposures. In contrast, the HD mutant line exhibited substantial deficits in net Mn uptake, even under basal conditions. Finally, to functionally evaluate a role for Fe transporters in the Mn uptake deficit, we examined Mn toxicity in the presence of saturating Fe(III) levels. Although Fe(III) exposure decreased Mn neurotoxicity, it did so equally for wild-type and mutant cells. Therefore, although Fe transporters contribute to Mn uptake and toxicity in the striatal cell lines, functional alterations in this pathway are insufficient to explain the strong Mn resistance phenotype of this HD cell model.
authors.affiliation: Neuroscience Graduate Program, Vanderbilt University Medical Center, Nashville, Tennessee 37232- 8552, USA.
authors.forename: B Blairanne
authors.initials: BB
authors.lastname: Williams
authors.forename: Gunnar F
authors.initials: GF
authors.lastname: Kwakye
authors.forename: Michal
authors.initials: M
authors.lastname: Wegrzynowicz
authors.forename: Daphne
authors.initials: D
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Aschner
authors.forename: Keith M
authors.initials: KM
authors.lastname: Erikson
authors.forename: Aaron B
authors.initials: AB
authors.lastname: Bowman
mesh_terms: D000818:Animals; D001692:Biological Transport; D027682:Cation Transport Proteins / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D006706:Homeostasis; D006801:Humans; D000071058:Huntingtin Protein; D006816:Huntington Disease / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism* / Q000633:toxicity*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D000071058:Huntingtin Protein; D007501:Iron; D008345:Manganese; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D000099236:Solute Carrier Family 11, Member 2; C506812:Htt protein, mouse