[Determination of six kinds of estrogens in cosmetics by liquid chromatograph-tandem mass spectrometry].
OBJECTIVE
To develop methods for the determination of six kinds of estrogens in cosmetics by Liquid Chromatograph-Tandem Mass Spectrometry (LC-MS/MS).


METHODS
Through choosing and optimizing experimental conditions, Methanol was chosen as the extracted solvent, super sonic extraction, then centrifuging, filtrating, and the filtrate was analysed by LC-MS/MS.


RESULTS
Using this method, the LC-MS/MS response to these six estrogens was linear in the range 0-1000 ng/ml. Level limit of detection of six estrogens were 2.4, 0.2, 0.7, 12, 6 and 5 ng/g, respectively, and level limit of quantification of six estrogens were 8, 0.7, 2.4, 40, 20 and 17 ng/g, respectively, the average spiking recovery rate was 85.2%-102.8%, and relative standard deviation (RSD) was within 2.76%-8.69%. 28 cosmetics were sampled and analyzed and no estrogens were measured.


CONCLUSIONS
This method possesses fairly high sensitivity and good specificity.
Wang
Jun
J
Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Zhou
Zhirong
Z
Lin
Shaobin
S
D002853:Chromatography, Liquid / Q000379:methods; D003358:Cosmetics / Q000737:chemistry*; D004958:Estradiol / Q000032:analysis; D004967:Estrogens / Q000032:analysis*; D004970:Estrone / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods
D003358:Cosmetics; D004967:Estrogens; D004970:Estrone; D004958:Estradiol