Neospora caninum: in vitro culture of tachyzoites in MCF-7 human breast carcinoma cells.
The development of Neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (MCF-7) as the host cell line. The extracellular NC-1 tachyzoites in MCF-7 cells were observed and counted daily for 6 consecutive days post-infection to establish the growth curve. The intracellular parasites were observed by acridine orange staining using Laser scanning confocal microscope. The results indicated that NC-1 tachyzoites invaded MCF-7 cells and multiplied intracellularly. The number of extracellular NC-1 tachyzoites started to increase rapidly around day 3 and reached the maximum number around day 4. Results from the present study suggested that MCF-7 cells were susceptible to NC-1 tachyzoites and could be used as an alternative cell line for in vitro studies.
Lv
Qiang
Q
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
Li
Jianhua
J
Gong
Pengtao
P
Xing
Shenyang
S
Zhang
Xichen
X
D000165:Acridine Orange; D000818:Animals; D001943:Breast Neoplasms; D018270:Carcinoma, Ductal, Breast; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal; D018688:Neospora / Q000254:growth & development* / Q000302:isolation & purification; D014709:Vero Cells
D005456:Fluorescent Dyes; D000165:Acridine Orange