Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal.
Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).
Hossain
Md Towhid
MT
Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Shibata
Takayuki
T
Kabashima
Tsutomu
T
Kai
Masaaki
M
D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D057166:High-Throughput Screening Assays; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008567:Membranes, Artificial; D051379:Mice; D010658:Phenylglyoxal / Q000378:metabolism*; D011328:Prions / Q000032:analysis* / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D055767:Immobilized Proteins; D008567:Membranes, Artificial; D011328:Prions; D010658:Phenylglyoxal