title: Polydispersity as a parameter for indicating the thermal stability of proteins by dynamic light scattering.
abstract: A physical parameter for predicting the thermal stability of proteins was provided by a new approach using dynamic light scattering (DLS). The relationship between the melting point measured by differential scanning calorimetry (DSC) and the polydispersity of the hydrodynamic diameter determined by DLS analysis was examined. Calmodulin (CaM) and concanavalin A (ConA) were used as model proteins. The melting point measured by DSC, an indicator for thermal stability, increased and the polydispersity decreased on binding of the proteins to specific ligands, suggesting that the polydispersity could be used an indicator to predict thermal stability. In addition, the increase of thermal stability that resulted from forming a complex could be quantified by polydispersity analysis even when the melting point changed only slightly.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka 819-0395, Japan. shiba.kouhei@sysmex.co.jp
authors.forename: Kohei
authors.initials: K
authors.lastname: Shiba
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
authors.forename: Etsuko
authors.initials: E
authors.lastname: Katoh
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xiang
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D003208:Concanavalin A / Q000737:chemistry; D008027:Light; D008297:Male; D008958:Models, Molecular; D011487:Protein Conformation; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D013696:Temperature; D044366:Transition Temperature
chemical_list: D002147:Calmodulin; D011506:Proteins; D003208:Concanavalin A; D002118:Calcium