Impact of developmental lead exposure on splenic factors.
Lead (Pb) is known to alter the functions of numerous organ systems, including the hematopoietic and immune systems. Pb can induce anemia and can lower host resistance to bacterial and viral infections. The anemia is due to Pb's inhibition of hemoglobin synthesis and Pb's induction of membrane changes, leading to early erythrocyte senescence. Pb also increases B-cell activation/proliferation and skews T-cell help (Th) toward Th2 subset generation. The specific mechanisms for many of the Pb effects are, as yet, not completely understood. Therefore, we performed gene expression analysis, via microarray, on RNA from the spleens of developmentally Pb-exposed mice, in order to gain further insight into these Pb effects. Splenic RNA microarray analysis indicated strong up-regulation of genes coding for proteolytic enzymes, lipases, amylase, and RNaseA. The data also showed that Pb affected the expression of many genes associated with innate immunity. Analysis of the microarray results via GeneSifter software indicated that Pb increased apoptosis, B-cell differentiation, and Th2 development. Direct up-regulation by Pb of expression of the gene encoding the heme-regulated inhibitor (HRI) suggested that Pb can decrease erythropoiesis by blocking globin mRNA translation. Pb's high elevation of digestive/catabolizing enzymes could generate immunogenic self peptides. With Pb's potential to induce new self-peptides and to enhance the expression of caspases, cytokines, and other immunomodulators, further evaluation of Pb's involvement in autoimmune phenomena, especially Th2-mediated autoantibody production, and alteration of organ system activities is warranted.
Kasten-Jolly
Jane
J
Laboratory of Clinical and Experimental Endocrinology and Immunology, Wadsworth Center, Albany, NY 12201-0509, USA.
Heo
Yong
Y
Lawrence
David A
DA
D000681:Amylases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D002918:Chymotrypsin / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D048788:Growth and Development / Q000187:drug effects; D006413:Hematopoietic System / Q000187:drug effects; D007113:Immunity, Innate; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D050797:STAT4 Transcription Factor / Q000378:metabolism; D050800:STAT6 Transcription Factor / Q000378:metabolism; D013154:Spleen / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects; D014357:Trypsin / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D050797:STAT4 Transcription Factor; D050800:STAT6 Transcription Factor; D007854:Lead; D012260:Ribonucleases; D000681:Amylases; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin