Extraction of lignans from flaxseed and evaluation of their biological effects on breast cancer MCF-7 and MDA-MB-231 cell lines.
Over the last decade, there has been an increasing interest in using flaxseed (Linum usitatissimum) in diet in order to improve nutritional and health status. Lignans are major components of flaxseed. Therefore an extraction procedure for lignans from flaxseed has been optimized. The influence of some parameters was investigated: first the preliminary extraction step with alcoholic solvent, and then the solvent polarity and pH of the extract. All these conditions affected the total lignan content, but the most critical variables were preliminary extraction and solvent polarity. The optimized procedure, consisting of a direct hydrolysis in hydrochloric acid (1 M) at 100 degrees C for 1 hour followed by an extraction with a mixture of ethyl acetate/hexane (90:10 vol/vol), was applied to 340 g of defatted flaxseed and resulted in the isolation of secoisolariciresinol and anhydrosecoisolariciresinol with a purity of 97% and 98%, respectively, as determined by high-performance liquid chromatography. The ability of these two compounds and that of secoisolariciresinol diglucoside to modulate the growth of human breast cancer MCF-7 and MDA-MB-231 cell lines was assessed. Our results show that lignans modulate development of breast cancer cells. The most intense effect was observed for anhydrosecoisolariciresinol, which significantly decreased cell growth at 50 and 100 microM.
Lehraiki
Abdelali
A
Biology of Plantes and Insects, EA 3900, Faculty of Pharmacy, University of Picardie Jules Verne, Amiens, France.
Attoumbré
Jacques
J
Bienaimé
Christophe
C
Matifat
Fabrice
F
Bensaddek
Lamine
L
Nava-Saucedo
Edmundo
E
Fliniaux
Marc-André
MA
Ouadid-Ahidouch
Halima
H
Baltora-Rosset
Sylvie
S
D001943:Breast Neoplasms / Q000188:drug therapy* / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005260:Female; D019597:Flax / Q000737:chemistry*; D006801:Humans; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
D017705:Lignans; D010936:Plant Extracts