Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis.
A Gram-negative, aerobic bacterium, designated strain R-40503(T), was isolated from mucus of the reef-builder coral Mussismilia hispida, located in the São Sebastião Channel, São Paulo, Brazil. Phylogenetic analyses revealed that strain R-40503(T) belongs to the genus Marinomonas. The 16S rRNA gene sequence similarity of R-40503(T) was above 97 % with the type strains of Marinomonas vaga, M. basaltis, M. communis and M. pontica, and below 97 % with type strains of the other Marinomonas species. Strain R-40503(T) showed less than 35 % DNA-DNA hybridization (DDH) with the type strains of the phylogenetically closest Marinomonas species, demonstrating that it should be classified into a novel species. Amplified fragment length polymorphism (AFLP), chemotaxonomic and phenotypic analyses provided further evidence for the proposal of a novel species. Concurrently, a close genomic relationship between M. basaltis and M. communis was observed. The type strains of these two species showed 78 % DDH and 63 % AFLP pattern similarity. Their phenotypic features were very similar, and their DNA G+C contents were identical (46.3 mol%). Collectively, these data demonstrate unambiguously that Marinomonas basaltis is a later heterotypic synonym of Marinomonas communis. Several phenotypic features can be used to discriminate between Marinomonas species. The novel strain R-40503(T) is clearly distinguishable from its neighbours. For instance, it shows oxidase and urease activity, utilizes l-asparagine and has the fatty acid C(12 : 1) 3-OH but lacks C(10 : 0) and C(12 : 0). The name Marinomonas brasilensis sp. nov. is proposed, with the type strain R-40503(T) ( = R-278(T)  = LMG 25434(T)  = CAIM 1459(T)). The DNA G+C content of strain R-40503(T) is 46.5 mol%.
Chimetto
Luciane A
LA
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.|Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Brazil.|Departament of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Brazil.
Cleenwerck
Ilse
I
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Brocchi
Marcelo
M
Departament of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas (UNICAMP), Brazil.
Willems
Anne
A
Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
De Vos
Paul
P
Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.|BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Thompson
Fabiano L
FL
Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Brazil.
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001482:Base Composition; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D053544:Marinomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S