title: [Study on rodent model to detect food allergenicity--C3H/HeJ mice model].
abstract: OBJECTIVE
  To study the feasibility of C3H/HeJ mice as the food allergenicity rodent model and explore the appropriate period of two different routes of exposure and the change of helper T lymphocyte cell.
  
  
  METHODS
  Allergenic protein ovalbumin (OVA) was administered intraperitoneally or orally to female C3H/HeJ mice for 35 days. Blood samples were taken on the 14th, 28th and 35th days from the orbital plexus and sera were obtained to determine OVA specific IgE (OVA sIgE). Spleen samples were obtained on the 35th day to detect the mRNA expression of Th2-type cytokines IL-4 and Th1-type cytokines IFN-gamma by real-time RT-PCR.
  
  
  RESULTS
  Compared with the control group, the concentration of OVA sIgE on 28th and 35th days were significantly increased in OVA group by gavage (P < 0.05). The concentration of OVA sIgE on 14th, 28th and 35th days were significantly higher than the control group when OVA was given intraperitoneally (P < 0.05). Compared with the control group, the mRNA expression of IL-4 of OVA group and OVA + Al(OH)3 group were significantly increased. The mRNA expression of IFN-gamma of OVA group and OVA + Al(OH) group were significantly lower than the control group.
  
  
  CONCLUSIONS
  C3H/HeJ mice might be used as a rodent model for the assessment of allergenicity of food. The appropriate period is the 14th day for the route of intraperitoneal injection and the 28th day for oral gavage, respectively.
authors.affiliation: Institute for Nutrition and Food Safety, China CDC, Beijing 100050, China. bananasunstar@163.com
authors.forename: Nana
authors.initials: N
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenming
authors.initials: W
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Zhang
authors.forename: Yufa
authors.initials: Y
authors.lastname: Peng
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xudong
authors.initials: X
authors.lastname: Jia
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D010047:Ovalbumin / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin