title: Gene expression changes associated with myocarditis and fibrosis in hearts of mice with chronic chagasic cardiomyopathy.
abstract: Chronic chagasic cardiomyopathy is a leading cause of heart failure in Latin American countries. About 30% of Trypanosoma cruzi-infected individuals develop this severe symptomatic form of the disease, characterized by intense inflammatory response accompanied by fibrosis in the heart. We performed an extensive microarray analysis of hearts from a mouse model of this disease and identified significant alterations in expression of approximately 12% of the sampled genes. Extensive up-regulations were associated with immune-inflammatory responses (chemokines, adhesion molecules, cathepsins, and major histocompatibility complex molecules) and fibrosis (extracellular matrix components, lysyl oxidase, and tissue inhibitor of metalloproteinase 1). Our results indicate potentially relevant factors involved in the pathogenesis of the disease that may provide new therapeutic targets in chronic Chagas disease.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil. milena@bahia.fiocruz.br
authors.forename: Milena Botelho Pereira
authors.initials: MB
authors.lastname: Soares
authors.forename: Ricardo Santana
authors.initials: RS
authors.lastname: de Lima
authors.forename: Leonardo Lima
authors.initials: LL
authors.lastname: Rocha
authors.forename: Juliana Fraga
authors.initials: JF
authors.lastname: Vasconcelos
authors.forename: Silvia Regina
authors.initials: SR
authors.lastname: Rogatto
authors.forename: Ricardo Ribeiro
authors.initials: RR
authors.lastname: dos Santos
authors.forename: Sanda
authors.initials: S
authors.lastname: Iacobas
authors.forename: Regina Coeli
authors.initials: RC
authors.lastname: Goldenberg
authors.forename: Dumitru Andrei
authors.initials: DA
authors.lastname: Iacobas
authors.forename: Herbert Bernard
authors.initials: HB
authors.lastname: Tanowitz
authors.forename: Antonio Carlos Campos
authors.initials: AC
authors.lastname: de Carvalho
authors.forename: David Conover
authors.initials: DC
authors.lastname: Spray
mesh_terms: D000818:Animals; D002598:Chagas Cardiomyopathy / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005355:Fibrosis / Q000276:immunology* / Q000473:pathology; D020869:Gene Expression Profiling; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009205:Myocarditis / Q000276:immunology* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000472:pathogenicity*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators