title: Antimycobacterial, antiprotozoal and cytotoxic potential of twenty-one brown algae (Phaeophyceae) from British and Irish waters.
abstract: In the continuation of our research on seaweeds, crude extracts of 21 brown algae collected from the south coast of England and the west coast of Ireland were screened for in vitro trypanocidal, leishmanicidal and antimycobacterial activities. Mammalian stages of a small set of parasitic protozoa; i.e. Trypanosoma brucei rhodesiense, T. cruzi and Leishmania donovani, and the tubercle bacillus Mycobacterium tuberculosis were used as test organisms. The extracts were also evaluated for selectivity by testing on a mammalian cell line (L6 cells). Only four extracts were moderately active against T. cruzi, whereas all algal extracts showed significant activity against T. brucei rhodesiense, with Halidrys siliquosa and Bifurcaria bifurcata (Sargassaceae) being the most potent (IC50 values 1.2 and 1.9 μg/mL). All algal extracts also displayed leishmanicidal activity, with H. siliquosa and B. bifurcata again being the most active (IC50s 6.4 and 8.6 μg/mL). When tested against M. tuberculosis, only the B. bifurcata extract was found to have some antitubercular potential (MIC value 64.0 μg/mL). Only three seaweed extracts, i.e. H. siliquosa, B. bifurcata and Cystoseira tamariscifolia showed some cytotoxicity. To our knowledge, this is the first study on the antiprotozoal and antimycobacterial activity of brown algae from British and Irish waters.
authors.affiliation: Department of Pharmaceutical and Biological Chemistry, Centre for Pharmacognosy and Phytotherapy, School of Pharmacy, University of London, London WC1N 1AX, UK.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Spavieri
authors.forename: Andrea
authors.initials: A
authors.lastname: Allmendinger
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Rosalyn
authors.initials: R
authors.lastname: Casey
authors.forename: Suzie
authors.initials: S
authors.lastname: Hingley-Wilson
authors.forename: Ajit
authors.initials: A
authors.lastname: Lalvani
authors.forename: Michael D
authors.initials: MD
authors.lastname: Guiry
authors.forename: Gerald
authors.initials: G
authors.lastname: Blunden
authors.forename: Deniz
authors.initials: D
authors.lastname: Tasdemir
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D004739:England; D020128:Inhibitory Concentration 50; D007494:Ireland; D007893:Leishmania donovani / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D014345:Trypanosoma / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D000995:Antitubercular Agents