Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting.
We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the electrode surface. The electrical field distribution is calculated in different electrode configurations by numerical simulations. Cell sorting shows high lysis efficiency, 99% of yeast cells sorted after lysis featuring dielectric properties similar to dead cells. A study of the potential device throughput is performed.
Mernier
Guillaume
G
Laboratoire de Microsystèmes LMIS4, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015, Lausanne, Switzerland. guillaume.mernier@epfl.ch
Piacentini
Niccolò
N
Braschler
Thomas
T
Demierre
Nicolas
N
Renaud
Philippe
P
D002452:Cell Count; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D056656:Lab-On-A-Chip Devices; D012441:Saccharomyces cerevisiae / Q000166:cytology