[Study of introduction apoptosis of hepatocarcinoma cell SMMC-7721 by anthraquinone extracted from rubiqmnone and molecular mechanism].
OBJECTIVE
To research the effect of anthraquinone extracted from rubiqmnone on growth inhibition, introduction apoptosis and expression of relative gene of bcl-2 of hepatocarcinoma cell SMMC 7721, and provide the effective target of gene therapy.


METHODS
The growth inhibition effect was detected by MTF. Morpholgy, DNA agarose gel electrophoresis and flow cytometry were used to observe the cell apoptosis. The effect of anthraquinone on bcl-2mRNA expression was analyzed by RT-PCR.


RESULTS
Anthraquinone could inhibit the growth of hepatocarcinoma cell SMMC 7721. The typical apoptosis cells were found by inverted microscope and common microscope. DNA agarose gel electrophoresis showed a typical apoptosis strip. G1 period of cell cycle had apoptosis peak of abnormal diploid by PI simple stain, and cell cycle stopped at G1 period. The apoptosis fuction of anthraquinone introdution hepatocarcinoma cell was further certified by Annexin V-FITC/PI. Anthraquinone could decrease the expression of bcl-2mRNA by RT-PCR.


CONCLUSIONS
Anthraquinone can significantly inhibit growth of hepatocarcinoma cell and induce apoptosis. The mocular mechanism may be related to down-regulating the expression of bcl-2 gene.
Wang
Yanshuang
Y
Department of Molecular Biology, Medical College of Beihua University, Jilin 132013, China. wangys5521@163.com
Luo
Su
S
D000818:Animals; D000880:Anthraquinones / Q000009:adverse effects / Q000302:isolation & purification / Q000627:therapeutic use*; D000999:Antlers / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000517:prevention & control; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003670:Deer; D015870:Gene Expression / Q000187:drug effects*; D019254:Genes, bcl-2 / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000880:Anthraquinones; C042834:9,10-anthraquinone