The expression of estrogen receptors in rat genioglossus muscle-derived satellite cells and its relationship to intracellular Ca(2+) mobilization.
Genioglossus (GG) is the most important pharyngeal dilator muscle in maintaining upper airway (UA) patency in human; therefore, its dysfunction plays an important role in pathogenesis of sleep-related breathing disorder. Recently, the expression of estrogen receptors (ERs) on mRNA and protein level has been evidenced in GG muscle; however, the cellular localization of two subtypes of ER in GG myoblasts remains unclear. The present study was designed to clarify the expression and cellular distribution of ERs in rat GG muscle-derived satellite cells (MDSCs) and further probe the effect of ERs expression on regulation of intracellular Ca(2+). The immunocytochemistry revealed positive staining for both ERalpha and ERbeta in nuclei and cytoplasm of GG MDSCs. Noticeably, positive signals for ERalpha and ERbeta were comparable in cytoplasm, whereas the positive staining of ERalpha in nuclear was obviously strong than that of ERbeta. More intriguingly, by using Fluo 4-AM as a fluorescent Ca(2+) indicator and 17beta-estradiol (E2) as a stimulant, we observed that the level of intracellular Ca(2+) was not affected by E2 application, which implied that Ca(2+) signaling may not be involved in ER-mediated estrogenic effects on GG MDSCs. Taken together, the present study clearly indicates the differential cellular localization of ERs in rat GG MDSCs; moreover, ER-mediated estrogenic effect in rat GG MDSCs bears no relationship to intracellular Ca(2+) mobilization. In addition, the GG MDSCs express both ERalpha and ERbeta and therefore, provide a suitable and convenient in vitro cell model for investigating the molecular mechanisms of estrogenic effects on rat GG muscle.
Guo
Tao
T
Department of Orthodontics, Fourth Military Medical University, Xi'an, Shaanxi, PR China.
Yu
Xiao-Ling
XL
Ding
Yin
Y
D000814:Aniline Compounds; D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000648:ultrastructure*; D047629:Estrogen Receptor beta / Q000648:ultrastructure*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D023421:Models, Animal; D010609:Pharyngeal Muscles / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology*; D014966:Xanthenes
D000814:Aniline Compounds; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D004958:Estradiol