Micromonospora humi sp. nov., isolated from peat swamp forest soil.
A novel actinomycete, strain P0402(T), was isolated from peat swamp forest soil collected in Thailand. Its taxonomic position was determined by using a polyphasic taxonomic approach. The chemotaxonomic characteristics of this strain matched those of the genus Micromonospora, i.e. the presence of meso-diaminopimelic acid and N-glycolyl muramic acid in the peptidoglycan, whole-cell sugar pattern D, phospholipid type II, and cellular fatty acid type 3b. Phylogenetic analysis based on 16S rRNA gene sequences revealed a close relationship between strain P0402(T) and Micromonospora coxensis JCM 13248(T) (99.0 % similarity), Micromonospora eburnea JCM 12345(T) (99.0 %), Micromonospora marina JCM 12870(T) (98.9 %), Micromonospora halophytica JCM 3125(T) (98.7 %), Micromonospora chalcea JCM 3031(T) (98.7 %), Micromonospora purpureochromogenes JCM 3156(T) (98.6 %) and Micromonospora aurantiaca JCM 10878(T) (98.5 %). It could be clearly distinguished from these type strains based on low levels of DNA-DNA relatedness and phenotypic differences. On the basis of the data presented, strain P0402(T) is suggested to represent a novel species of the genus Micromonospora, for which the name Micromonospora humi sp. nov. is proposed. The type strain is P0402(T) ( = JCM 15292(T)  = PCU 315(T)  = TISTR 1883(T)).
Songsumanus
Apakorn
A
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Tanasupawat
Somboon
S
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Thawai
Chitti
C
Department of Biology and Microbiol Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology, Ladkrabang, Bangkok 10520, Thailand.
Suwanborirux
Khanit
K
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Kudo
Takuji
T
Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
D004269:DNA, Bacterial / Q000235:genetics; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013785:Thailand; D014197:Trees / Q000382:microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S