title: Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.).
abstract: Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.
authors.affiliation: Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran; E-Mails: m_pirseyedi@abrii.ac.ir (S.M.P.); s.valizadegan@gmail.com (S.V.); ghaffary@abrii.ac.ir (M.R.G.); mparvaneh59@yahoo.com (P.M.); mzeinolabedini@abrii.ac.ir (M..Z.); khayam@abrii.ac.ir (S.M.K.N.).
authors.forename: Seyed Mostafa
authors.initials: SM
authors.lastname: Pirseyedi
authors.forename: Sahar
authors.initials: S
authors.lastname: Valizadehghan
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mardi
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ghaffari
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Mahmoodi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Zahravi
authors.forename: Mehrshad
authors.initials: M
authors.lastname: Zeinalabedini
authors.forename: Seyed Mojtaba Khayam
authors.initials: SM
authors.lastname: Nekoui
mesh_terms: D000483:Alleles; D056426:Genetic Loci; D015698:Genomic Library; D029561:Lythraceae / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic