title: Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range.
abstract: A rapid positive screening system for identifying recombinant baculovirus with an expanded host range was developed by using an actin promoter-dependent fluorescent protein gene expression cassette. The expression of recombinant protein regulated by the polyhedrin promoter was not affected by the insertion of the cassette in the baculovirus genome.
authors.affiliation: Research Team for Advanced Biologicals, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. satochan@affrc.go.jp
authors.forename: Satoko
authors.initials: S
authors.lastname: Watanabe
authors.forename: Masato
authors.initials: M
authors.lastname: Ohta
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kokuho
authors.forename: Hajime
authors.initials: H
authors.lastname: Mori
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
mesh_terms: D016367:Baculoviridae / Q000235:genetics* / Q000302:isolation & purification*; D015967:Gene Expression Regulation, Viral; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000235:genetics; D014773:Virology / Q000379:methods*
chemical_list: D011994:Recombinant Proteins