title: Assessing genetic differentiation in geographic populations of Labeo calbasu using allozyme markers.
abstract: The population structure of Labeo calbasu from 11 rivers belonging to the Indus, Ganges, Bhima, Mahanadi, and Godavari basins was investigated using allozyme marker systems. Seven out of 20 allozyme loci (35%) were polymorphic (P < 0.99). Both probability and score tests indicated significant deviation of genotype proportions from Hardy-Weinberg expectations at two loci, XDH* (Mahanadi, Bhima, and Godavari) and G6PDH* (Mahanadi). A pairwise genetic homogeneity test and F (ST) values indicated a low-to-moderate level (0.0515) of genetic structuring in the wild population of L. calbasu. AMOVA analysis also indicated moderate differentiation among the samples from different river basins. Analysis for genetic bottleneck was performed under the infinite allele model. The study revealed nine genetic stocks of L. calbasu from the natural population across Indian rivers. Evidence of genetic bottlenecks in some rivers was also revealed.
authors.affiliation: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, UP, India.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Singh
authors.forename: Kuldeep K
authors.initials: KK
authors.lastname: Lal
authors.forename: Vindhya
authors.initials: V
authors.lastname: Mohindra
authors.forename: Peyush
authors.initials: P
authors.lastname: Punia
authors.forename: Rama S
authors.initials: RS
authors.lastname: Sah
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Mishra
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.forename: B N
authors.initials: BN
authors.lastname: Mishra
authors.forename: W S
authors.initials: WS
authors.lastname: Lakra
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000145:classification / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D058974:Phylogeography; D011110:Polymorphism, Genetic; D011156:Population Density; D045483:Rivers
chemical_list: D005819:Genetic Markers; D007527:Isoenzymes