title: Preliminary evaluation of a novel strong/osteoinductive calcium phosphate cement.
abstract: We developed a novel calcium phosphate cement (CPC) by combining the silk fibroin and osteogenic supplements (β-glycerophosphate, ascorbic acid, and dexamethasone) with α-tricalcium phosphate cement. Mesenchymal stem cells (MSCs) were cultured on the novel CPC scaffold. Results showed that the novel CPC scaffold was biocompatible and favorable for the adhesion, spreading, and proliferation of MSCs. Osteogenic differentiation of MSCs was confirmed by high osteocalcin content and elevated gene expressions of bone markers, such as alkaline phosphatase, collagen type I, and osteocalcin. Therefore, the novel CPC scaffold may be potentially useful for implant fixation and more rapid new bone formation in moderate load-bearing applications.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University Chengdu, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Qu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Chen
authors.forename: Jidong
authors.initials: J
authors.lastname: Li
authors.forename: Kuangyun
authors.initials: K
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: Man
mesh_terms: D000818:Animals; D001483:Base Sequence; D001696:Biomechanical Phenomena; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers / Q000235:genetics; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D016474:Weight-Bearing
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D017931:DNA Primers; C485828:alpha-tricalcium phosphate; D015675:Osteocalcin; D005349:Fibroins