title: Structural identification of recombinant human CD83 mutant variant as a potent therapeutic protein.
abstract: The formation of aberrant disulfide bonds is a structural consideration for the manufacturing of the extracellular domain of human CD83 (hCD83ext), a potential therapeutic protein. In certain instances, hCD83ext protein products, even when stored frozen, tended to dimerize or even multimerize through the formation of aberrant intermolecular disulfide bonds. Herein, we discovered an analytical inconsistency and applied a modified sample preparation protocol for proper structural analysis of hCD83ext products which are heterologously expressed in Escherichia coli and subsequently purified. In addition, a mutant derivative with the Cys100Ser mutation was identified as an improved version which did not form dimers or multimers. The identification of this mutant variant as a more potent therapeutic protein than other hCD83ext species demonstrated that the structural variation associated with disulfide bond formation can be a critical issue for rigorous control of the quality and bioactivity of therapeutic proteins. The application of this mutant variant for protein therapeutics is currently under exploration.
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Niju
authors.initials: N
authors.lastname: Narayanan
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Brand
authors.forename: Charles A
authors.initials: CA
authors.lastname: Nicolette
authors.forename: Miren
authors.initials: M
authors.lastname: Baroja
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Arp
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Murray
authors.initials: M
authors.lastname: Moo-Young
authors.forename: C Perry
authors.initials: CP
authors.lastname: Chou
mesh_terms: D019943:Amino Acid Substitution; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics* / Q000627:therapeutic use; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D014644:Genetic Variation; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics* / Q000627:therapeutic use; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000627:therapeutic use; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000627:therapeutic use; D012694:Serine / Q000378:metabolism; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D004220:Disulfides; D005982:Glutathione Transferase; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D012694:Serine; D014841:von Willebrand Factor; D000097802:CD83 Antigen