title: Immune response of BALB/c mice against an experimental vaccine of Alum precipitated autoclaved Leishmania major (Alum-ALM) mixed with BCG or Mycobacterium vaccae.
abstract: Immune response in BALB/c mice immunized 3 times with different doses (50 μg or 200 μg of protein) of Alum precipitated autoclaved Leishmania major (Alum-ALM) mixed with either BCG (1x10(7); CFU) or different doses of killed Mycobacterium vaccae (1x10(6), 1x10(7)) was assessed. Mice immunized with low dose of Alum-ALM mixed with either BCG or low M. vaccae showed a significantly higher IFN-gamma production and a lower IL-4 level and a significantly lower parasite burden compared to the control PBS injected group. It seems that immunization with a low dose of Alum-ALM mixed with an adjuvant induces a Th1 type of immune response in susceptible BALB/c mice.
authors.affiliation: Medical Parasitology and Mycology Department, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Nateghi Rostami
authors.forename: H
authors.initials: H
authors.lastname: Keshavarz
authors.forename: A
authors.initials: A
authors.lastname: Khamesipour
mesh_terms: D000534:Alum Compounds; D000818:Animals; D000913:Antibodies, Protozoan; D011232:Chemical Precipitation; D006968:Hypersensitivity, Delayed; D054332:Leishmaniasis Vaccines / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009161:Mycobacterium / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D000534:Alum Compounds; D000913:Antibodies, Protozoan; D054332:Leishmaniasis Vaccines; D015164:Vaccines, Inactivated; C416262:autoclaved Leishmania major vaccine; C041524:aluminum sulfate