title: Lipoglycans of Mycobacterium tuberculosis: isolation, purification, and characterization.
abstract: In this chapter, we describe in detail the steps involved in isolation and characterization of lipoglycans from Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition, procedures involved in structural analysis such as immunoblotting with mAb CS-35 or CS-40, gas chromatography, gas chromatography/mass spectrometry, nuclear magnetic resonance spectroscopy, and endoarabinanase digestion followed by high-pH anion exchange chromatography and two-dimensional gel electrophoresis are presented.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: Libin
authors.initials: L
authors.lastname: Shi
authors.forename: Jordi B
authors.initials: JB
authors.lastname: Torrelles
authors.forename: Delphi
authors.initials: D
authors.lastname: Chatterjee
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008967:Molecular Biology / Q000379:methods*; D020102:Mycobacterium smegmatis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*
chemical_list: D008070:Lipopolysaccharides