[The significance of TGF-beta expression in scar in adenosine receptor A(2A) knockout mice].
OBJECTIVE
To discuss the mechanism of scar hypertrophy in adenosine receptor A(2A) (A(2A) R) knockout mice.


METHODS
Animal models of hypertrophic scar were established in 12 A(2A) R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining. The hydroxyproline (HYP) in the scar was measured colorimetrically. The TGF-beta expression was tested by Western blotting method.


RESULTS
The hypertrophic scar in wild-type mice was more severe than that in knockout mice. Compared with self-control, the increase of the thickness and the size of transverse section of hypertrophic scar was markedly higher in wild-type group than in the knockout group (P < 0.01). There was significant difference in HYP content between the two groups (P < 0.01). Compared with self-control, the increase of TGF-beta expression in wild-type group was much more than that in knockout group (P < 0.01).


CONCLUSIONS
The TGF-beta expression decreases in the A(2A) R knockout mice. The scar hypertrophy is also much less in the A(2A) R knockout mice.
Xiao
Hu
H
Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Ran
Li
L
Zhuo
Ying-ying
YY
Wang
De-chang
DC
Huo
Ran
R
Wang
Yi-bing
YB
Feng
Yong-qiang
YQ
Li
Qiang
Q
D000818:Animals; D002921:Cicatrix / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D043705:Receptor, Adenosine A2A / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
D043705:Receptor, Adenosine A2A; D016212:Transforming Growth Factor beta