[Isolation, identification and insecticidal activity of endophyte from Achnatherum inebrians].
OBJECTIVE
To study endophyte species of Achnatherum inebrians and to screen strains with insecticidal activity against cotton insect.


METHODS
We isolated endophytic from roots,stems,leaves and seeds of health A. inebrians by grinding separation method and identified by a dual approach of morphological and physiological observation and 16S rDNA gene (for bacteria) and ITS sequence (for fungi) based molecular identification. Then,those endophytes were inoculated into liquid media for fermentation and the crude extracts were used to test insecticidal activities by slide disc immersion and nebulization methods.


RESULTS
We isolated bacteria species classified into 8 genera of Bacillus, Streptomyces, Corynebacterium, Phyllobacterium, sphingomonnas, Paenibacillus, Pseudomonas, Acinetobacter and 2 fungi of Claviceps purpure and Claviceps Chaetomium. Of them, the strain Streptomyces rochei (GA) and Claviceps purpurea (PF-2) had more than 85% of mortality to cotton aphis.


CONCLUSIONS
Two strains of PF-2 and GA associated within the A. inebrians had significant insecticidal activity to cotton aphis (Aphis gossypii), which may provide a new biological resource to explore new microbial insecticide.
Zhang
Xuebing
X
College of Food Science of Xinjiang Agricultural University, Urumqi 830052, China. zhangxuebing1983.student@sina.com.cn
Shi
Yingwu
Y
Wang
Xiaoxia
X
Zhang
Wei
W
Lou
Kai
K
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification / Q000472:pathogenicity; D002967:Claviceps / Q000302:isolation & purification* / Q000472:pathogenicity; D004271:DNA, Fungal / Q000302:isolation & purification*; D005658:Fungi / Q000302:isolation & purification / Q000472:pathogenicity; D007313:Insecta / Q000187:drug effects / Q000382:microbiology; D007306:Insecticides; D056507:Paenibacillus / Q000302:isolation & purification* / Q000472:pathogenicity; D018515:Plant Leaves / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology*
D004271:DNA, Fungal; D007306:Insecticides