Development and evaluation of an open source Delphi-based software for morphometric quantification of liver fibrosis.
BACKGROUND
Computer-based morphometry can minimize subjectivity in the assessment of liver fibrosis. An image processing program was developed with Delphi for the quantification of fibrosis in liver tissue samples stained with Sirius Red. Bile duct ligated and sham operated wild type C57BL/6 mice served as a model of time-dependent induction of liver fibrosis. Formation of fibrosis was determined with the developed software at day 0, 3, 7, 10, 14, 20, 30 and 60. The results were compared to a semi-quantitative scoring system.


RESULTS
Quantitative accumulation of collagen fibres was observed from day 3 to day 14, with a slight further increase thereafter. During ongoing fibrogenesis, there was a significant elevation of alanine aminotransferase (ALT), aspartate transaminase (AST) and bilirubin. The results from our computer-based morphometric analysis were highly correlated with the results that were obtained in a standardized pathology semi-quantitative scoring system (R 2 = 0.89, n = 38).


CONCLUSIONS
Using our Delphi-based image analysing software, the morphometric assessment of fibrosis is as precise as semi-quantitative scoring by an experienced pathologist. This program can be a valuable tool in any kind of experimental or clinical setting for standardized quantitative assessment of fibrosis.
Huss
Sebastian
S
Institute of Pathology, University of Bonn, Bonn, Germany. sebastian.huss@ukb.uni-bonn.de.
Schmitz
Jörg
J
Goltz
Diane
D
Fischer
Hans-Peter
HP
Büttner
Reinhard
R
Weiskirchen
Ralf
R