title: Identification of novel antioxidative peptides derived from a thermolytic hydrolysate of ovotransferrin by LC-MS/MS.
abstract: Ovotransferrin is a glycoprotein well-known for its iron-binding property. Ovotransferrin was reported to have antioxidative properties, but the presence of antioxidant peptides within the protein has not been reported. The purpose of the study was to characterize the antioxidant peptides within ovotransferrin. Ovotransferrin was sonicated and hydrolyzed by thermolysin, and peptides from the hydrolysate were fractionated by ion-exchange fast protein liquid chromatography and reversed-phase high-performance liquid chromatography. Fourteen peptides derived from ovotransferrin were characterized using LC-MS/MS, and their oxygen radical absorbance capacity (ORAC) values were determined using synthetic peptides. Two tetrapeptides (Trp-Asn-Ile-Pro and Gly-Trp-Asn-Ile) showed the highest antioxidant activity. Interestingly, the addition of amino acid residues to either the N or C terminus of the two peptides decreased the antioxidant activity, suggesting that the motif of Trp-Asn-Ile is responsible for the high antioxidant activity.
authors.affiliation: 4-10 Agriculture Forestry Centre, Department of Agricultural and Food Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Shen
authors.forename: Baljot
authors.initials: B
authors.lastname: Chahal
authors.forename: Kaustav
authors.initials: K
authors.lastname: Majumder
authors.forename: Sun-Jong
authors.initials: SJ
authors.lastname: You
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003207:Conalbumin / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry; D013820:Thermolysin / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010455:Peptides; D003207:Conalbumin; D013820:Thermolysin