Thymidine kinase 1 deficient cells show increased survival rate after UV-induced DNA damage.
Balanced deoxynucleotide pools are known to be important for correct DNA repair, and deficiency for some of the central enzymes in deoxynucleotide metabolism can cause imbalanced pools, which in turn can lead to mutagenesis and cell death. Here we show that cells deficient for the thymidine salvage enzyme thymidine kinase 1 (TK1) are more resistant to UV-induced DNA damage than TK1 positive cells although they have thymidine triphosphate (dTTP) levels of only half the size of control cells. Our results suggest that higher thymidine levels in the TK- cells caused by defect thymidine salvage to dTTP protects against UV irradiation.
Skovgaard
T
T
Department of Science Systems and Models, Roskilde University, Roskilde, Denmark.
Rasmussen
L J
LJ
Munch-Petersen
B
B
D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects*; D006801:Humans; D013937:Thymidine Kinase / Q000172:deficiency* / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects*
D013937:Thymidine Kinase; C092717:thymidine kinase 1