Plant structure visualization by high-resolution X-ray computed tomography.
New developments in high-resolution X-ray computed tomography (HRXCT) are promising for the broader application of this non-destructive imaging method in plant sciences. Here, we demonstrate how detailed three-dimensional morphological traits can be extracted rapidly from in vivoArabidopsis thaliana seedlings without sample manipulation. Furthermore, ex vivo scanning at sub-micron resolution allows the quantification and visualization of the cellular organization of plant tissue samples, making HRXCT a desired tool in developmental plant biology.
Dhondt
Stijn
S
Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium.
Vanhaeren
Hannes
H
Van Loo
Denis
D
Cnudde
Veerle
V
Inzé
Dirk
D
D017360:Arabidopsis / Q000033:anatomy & histology*; D001901:Botany / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D036226:Seedlings / Q000033:anatomy & histology / Q000254:growth & development; D014057:Tomography, X-Ray Computed