T-vector and in vivo recombination as tools to construct a large antibody library of breast cancer.
The emergence of phage antibody libraries is an important advance in the field of antibody engineering. It provides a useful methodology to produce human antibodies and has the potential to replace traditional hybridoma technology. In our research, we used T-vector and in vivo recombination to construct a large antibody library from breast cancer patients. The use of T-vector considerably increased the cloning efficiency, and the diversity of the library could be increased easily using in vivo recombination. Taken together, a combination of these two techniques might be valuable in constructing a large antibody library.
Lv
Yong-Gang
YG
Department of Vascular and Endocrine Surgery, First Affiliated Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
Wang
Ting
T
Yuan
Shi-Fang
SF
Li
Nan-Lin
NL
Chen
Jiang-Hao
JH
Zhao
Ai-Zhi
AZ
Ling
Rui
R
Wang
Ling
L
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D019151:Peptide Library; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D019151:Peptide Library