title: Analysis of the vp2 gene sequence of a new mutated mink enteritis parvovirus strain in PR China.
abstract: BACKGROUND
  Mink enteritis virus (MEV) causes a highly contagious viral disease of mink with a worldwide distribution. MEV has a linear, single-stranded, negative-sense DNA with a genome length of approximately 5,000 bp. The VP2 protein is the major structural protein of the parvovirus encoded by the vp2 gene. VP2 is highly antigenic and plays important roles in determining viral host ranges and tissue tropisms. This study describes the bionomics and vp2 gene analysis of a mutated strain, MEV-DL, which was isolated recently in China and outlines its homologous relationships with other selected strains registered in Genbank.
  
  
  RESULTS
  The MEV-DL strain can infect F81 cells with cytopathic effects. Pig erythrocytes were agglutinated by the MEV-DL strain. The generation of MEV-DL in F81 cells could infect mink within three months and cause a disease that was similar to that caused by wild-type MEV. A comparative analysis of the vp2 gene nucleotide (nt) sequence of MEV-DL showed that this was more than 99% homologous with other mink enteritis parvoviruses in Genbank. However, the nucleotide residues at positions 1,065 and 1,238 in the MEV-DL strain of the vp2 gene differed from those of all the other MEV strains described previously. It is noteworthy that the mutation at the nucleotide residues position 1,238 led to Asp/Gly replacement. This may lead to structural changes. A phylogenetic tree and sequence distance table were obtained, which showed that the MEV-DL and ZYL-1 strains had the closest inheritance distance.
  
  
  CONCLUSIONS
  A new variation of the vp2 gene exists in the MEV-DL strain, which may lead to structural changes of the VP2 protein. Phylogenetic analysis showed that MEV-DL may originate from the ZYL-1 strain in DaLian.
authors.affiliation: College of Animal Science and Veterinary Medicine, JiLin University, 5333 Xi 'an Road, Changchun 130062, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zuo
authors.forename: Jiahui
authors.initials: J
authors.lastname: Rao
authors.forename: Huihui
authors.initials: H
authors.lastname: Xu
authors.forename: Liming
authors.initials: L
authors.lastname: Ma
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Yuping
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuehui
authors.initials: X
authors.lastname: Cai
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China; D005243:Feces / Q000821:virology; D008907:Mink; D053489:Mink Viral Enteritis / Q000821:virology*; D053490:Mink enteritis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D015678:Viral Structural Proteins