The influence of incorporating lipids or liposaccharides on the particle size of peptide therapeutics.
The characterization of peptide-based drugs is essential to obtain information about their potential suitability. In this study, a therapeutic peptide epitope alone or in combination with a lipid or liposaccharide moiety were assessed to determine their particle sizes by diffusion ordered NMR spectroscopy, dynamic light scattering, and mathematical expressions. These methods were compared and their suitability for different types of peptides is discussed herein. When compared with the mathematical expressions, we found that the NMR method resulted in a particle size that was consistent with the radius of the peptide monomer. The dynamic light scattering method showed that when lipids were conjugated to the peptide epitope, the resulting particles had a larger sized distribution compared with the peptide alone. These experiments provided information which can be applied when formulating these peptides as drugs.
Coles
Daniel J
DJ
The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD 4072, Australia.
Simerska
Pavia
P
Fujita
Yoshio
Y
Toth
Istvan
I
D008055:Lipids / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*
D008055:Lipids; D008070:Lipopolysaccharides; D010455:Peptides