Expression of human leucocyte antigen-G primarily targets affected skin of patients with psoriasis.
BACKGROUND
The nonclassical human leucocyte antigen (HLA)-G molecule has been well recognized as a tolerogenic molecule and few studies have evaluated the role of the molecule in inflammatory cutaneous autoimmune diseases.


OBJECTIVE
To evaluate the expression of HLA-G in skin specimens of patients with psoriasis and to analyse its correlation with epidemiological and clinical variables.


METHODS
Thirty untreated patients with psoriasis and 32 healthy individuals were enrolled. Immunohistochemistry was applied to identify HLA-G expression in formalin-fixed paraffin-embedded cutaneous skin biopsies.


RESULTS
Soluble and membrane-bound HLA-G expression was detected in 30 (90%) of the skin specimens from patients presenting clinical and histopathological features of psoriasis. Although infiltrating lymphomononuclear cells of the dermis exhibited HLA-G expression, the epidermis was primarily targeted. HLA-G expression was also observed in 27% (three of 11) of the specimens that exhibited no clinical and histopathological features of psoriasis (nonaffected areas). In contrast, skin specimens obtained from healthy individuals exhibited no HLA-G expression (P < 0·0001). The intensity of HLA-G expression was not associated with type I/II psoriasis, Psoriasis Area and Severity Index score or clinical forms.


CONCLUSIONS
As the HLA-G molecule was consistently expressed in affected and, to a lesser extent, in nonaffected areas of untreated patients with psoriasis, irrespective of the severity of the clinical variants, one may hypothesize that the presence of HLA-G may be responsible, at least in part, for the regulation of autoimmune effector cells.
Cardili
R N
RN
Department of Internal Medicine, Division of Dermatology, School of Medicine of Ribeirão Preto, University of São Paulo, USP, Ribeirão Preto, São Paulo, Brazil.
Alves
T G
TG
Freitas
J C O C
JC
Soares
C P
CP
Mendes-Junior
C T
CT
Soares
E G
EG
Donadi
E A
EA
Silva-Souza
C
C
D000293:Adolescent; D000328:Adult; D000368:Aged; D004817:Epidermis / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000378:metabolism*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011565:Psoriasis / Q000276:immunology*; D012720:Severity of Illness Index; D012867:Skin / Q000276:immunology*; D055815:Young Adult
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I