Autoantibody against survivin in patients with systemic sclerosis.
OBJECTIVE
In patients with systemic sclerosis (SSc), to determine concentrations of antibodies against survivin and their clinical association with SSc, and to evaluate serum survivin concentrations.


METHODS
Anti-survivin antibody was examined by ELISA and immunoblotting using human recombinant survivin. Serum survivin levels were assessed by ELISA.


RESULTS
IgG but not IgM anti-survivin antibody levels in patients with SSc were significantly higher than those in healthy controls and patients with systemic lupus erythematosus (SLE). When cutoff values were set as mean + 2 SD of control, IgG anti-survivin antibodies were positive in 41% (25/61) of patients with SSc, while they were detected in only 1 healthy individual (3%, 1/29) and 1 patient with SLE (5%, 1/20). Regarding the clinical correlation, patients with SSc who were positive for IgG anti-survivin antibody exhibited significantly longer disease duration than those who were negative. Immunoblotting analysis confirmed the presence of anti-survivin antibody in sera from patients with SSc. Serum survivin levels in patients with SSc were also significantly higher than in controls and patients with SLE.


CONCLUSIONS
Our results suggest that autoantibody against survivin is generated in patients with SSc, especially those with long disease duration.
Koike
Yuta
Y
Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Muroi
Eiji
E
Yoshizaki
Ayumi
A
Ogawa
Fumihide
F
Yanaba
Koichi
K
Takenaka
Motoi
M
Shimizu
Kazuhiro
K
Sato
Shinichi
S
D000328:Adult; D044466:Asian People; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015853:Cysteine Proteinase Inhibitors / Q000276:immunology*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000097:blood / Q000276:immunology; D000077022:Survivin
D001323:Autoantibodies; C107489:BIRC5 protein, human; D015853:Cysteine Proteinase Inhibitors; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin