A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers.
The short arm of rye chromosome 1R (1RS) is introduced into many common wheat cultivars because of its agronomic importance. The gametocidal system has been used to produce dissection lines carrying segments of rye chromosome 1R. We focused on establishing more dissection lines for 1RS and on obtaining PCR-based markers specific to 1RS. We established 66 1RS dissection lines carrying 1RS segments of chromosome 1R derived from a common wheat cultivar 'Burgas 2' and obtained 27 markers. We conducted a PCR analysis using the dissection lines and markers, and divided 1RS into 17 regions separated by the breakpoints. Comparison of the 'Burgas 2' 1RS map with another map of 1RS derived from 'Imperial' rye implied a restructuring between the 2 1RS chromosomes.
Gyawali
Y P
YP
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Nasuda
S
S
Endo
T R
TR
D001483:Base Sequence; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D012434:Secale / Q000235:genetics*; D013045:Species Specificity; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers