title: A theoretical study to establish the relationship between the three-dimensional structure of triose-phosphate isomerase of Giardia duodenalis and point mutations in the respective gene.
abstract: Predicting how point mutations in genes alter the tertiary and quarternary structure of proteins is central to a number of areas of molecular biology and has implications in relation to the function and evolution of molecules. In the present study, we theoretically assessed the effects of 20 point mutations detected previously in a region of the triose-phosphate isomerase gene (tpi) of the protozoan Giardia duodenalis on the three-dimensional structure of the 'wild-type' protein (TPI). Amino acid substitutions arising from codon variations were mainly located at surface-accessible sites or in hydrophobic pockets of TPI. None of the substitutions was predicted to exert a significant change to the fold or functionality of the enzyme, with the exception of one alteration (Arg100). Almost all substitutions were either conservative or semi-conservative, and retained or even improved the expected stability of the fold. Overall, the findings provide support for the "neutral theory", which contends that evolution at the molecular level is not solely shaped by "Darwinian selection but also by random drift of selectively neutral or nearly neutral mutants".
authors.affiliation: Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Nolan
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D019143:Evolution, Molecular; D016829:Giardia lamblia / Q000201:enzymology / Q000235:genetics*; D008958:Models, Molecular; D017354:Point Mutation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics*; D014305:Triose-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins; D014305:Triose-Phosphate Isomerase