Preliminary evaluation of a novel strong/osteoinductive calcium phosphate cement.
We developed a novel calcium phosphate cement (CPC) by combining the silk fibroin and osteogenic supplements (β-glycerophosphate, ascorbic acid, and dexamethasone) with α-tricalcium phosphate cement. Mesenchymal stem cells (MSCs) were cultured on the novel CPC scaffold. Results showed that the novel CPC scaffold was biocompatible and favorable for the adhesion, spreading, and proliferation of MSCs. Osteogenic differentiation of MSCs was confirmed by high osteocalcin content and elevated gene expressions of bone markers, such as alkaline phosphatase, collagen type I, and osteocalcin. Therefore, the novel CPC scaffold may be potentially useful for implant fixation and more rapid new bone formation in moderate load-bearing applications.
Qu
Yili
Y
State Key Laboratory of Oral Diseases, Sichuan University Chengdu, China.
Yang
Yang
Y
Li
Juan
J
Chen
Zhiqing
Z
Li
Jidong
J
Tang
Kuangyun
K
Man
Yi
Y
D000818:Animals; D001483:Base Sequence; D001696:Biomechanical Phenomena; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D017931:DNA Primers / Q000235:genetics; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D016474:Weight-Bearing
D001843:Bone Cements; D002130:Calcium Phosphates; D017931:DNA Primers; C485828:alpha-tricalcium phosphate; D015675:Osteocalcin; D005349:Fibroins