Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
Lee
Xiaoyun
X
Département de Microbiologie Fondamentale, Université de Lausanne, Lausanne, Switzerland.
Fox
Aine
A
Sufrin
Janice
J
Henry
Hugues
H
Majcherczyk
Paul
P
Haas
Dieter
D
Reimmann
Cornelia
C
D000613:Aminobutyrates / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000963:Antimetabolites / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics*; D023061:Gene Order; D005798:Genes, Bacterial; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*
D000613:Aminobutyrates; D000900:Anti-Bacterial Agents; D000963:Antimetabolites; C000264:2-amino-4-methoxy-3-butenoic acid