title: Techniques of dental DNA extraction: Some operative experiences.
abstract: This study presents and discusses the possibility of multiple dental DNA extraction at different times using an endodontic technique. We chose a mixed sample of twenty teeth (decayed, filled, different kinds of teeth, etc.) and performed two different accesses to the pulp cavity to collect samples of dental tissue useful for DNA extraction. The Identifiler(®) and Minifiler(®) kits were used respectively for the first and second genetic analysis. The research's most relevant findings are the possibility of successfully repeating dental DNA extractions, and that a dental element can be considered as a DNA source that can be reused even after prolonged time elapses. The study's results document a simple and efficient methodology for dental identification based on the choice of dental elements, endodontic techniques and the use of the Minifiler(®) kit for genetic analysis.
authors.affiliation: Department of Anatomy, Histology and Legal Medicine, Legal-Medicine Section, University of Florence, Viale Morgagni 85, 50134 Florence, Italy. pinchi@unifi.it
authors.forename: Vilma
authors.initials: V
authors.lastname: Pinchi
authors.forename: Francesca
authors.initials: F
authors.lastname: Torricelli
authors.forename: A Lucia
authors.initials: AL
authors.lastname: Nutini
authors.forename: Marco
authors.initials: M
authors.lastname: Conti
authors.forename: Sara
authors.initials: S
authors.lastname: Iozzi
authors.forename: Gian-Aristide
authors.initials: GA
authors.lastname: Norelli
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003782:Dental Pulp / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005553:Forensic Dentistry / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D014081:Tooth Extraction
chemical_list: D004247:DNA