A new technique for seeding chondrocytes onto solvent-preserved human meniscus using the chemokinetic effect of recombinant human bone morphogenetic protein-2.
Many investigators are currently studying the use of decellularized tissue allografts from human cadavers as scaffolds onto which patients' cells could be seeded, or as carriers for genetically engineered cells to aid cell transplantation. However, it is difficult to seed cells onto very dense regular connective tissue which has few interstitial spaces. Here, we discuss the development of a chemotactic cell seeding technique using solvent-preserved human meniscus. A chemokinetic response to recombinant human bone morphogenetic protein-2 (rhBMP-2) was observed in a monolayer culture of primary chondrocytes derived from femoral epiphyseal cartilage of 2-day-old rats. The rhBMP-2 significantly increased their migration upto 10 ng/ml in a dose-dependent manner. When tested with solvent-preserved human meniscus as a scaffold, which has few interstitial spaces, rhBMP-2 was able to induce chondrocytes to migrate into the meniscus. After a 3-week incubation, newly-formed cartilaginous extracellular matrix was synthesized by migrated chondrocytes throughout the meniscus, down to a depth of 3 mm. These findings demonstrate that rhBMP-2 may be a natural chemokinetic factor in vivo, which induces migration of proliferative chondrocytes into the narrow interfibrous spaces. Our results suggest a potential application of rhBMP-2 for the designed distribution of chondrocytes into a scaffold to be used for tissue engineering.
Minehara
Hiroaki
H
Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Japan.
Urabe
Ken
K
Naruse
Kouji
K
Mehlhorn
Alexander T
AT
Uchida
Kentaroo
K
Südkamp
Norbert P
NP
Itoman
Moritoshi
M
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D005269:Femur / Q000166:cytology; D006132:Growth Plate / Q000166:cytology; D006801:Humans; D008592:Menisci, Tibial / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism; D012997:Solvents; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D012997:Solvents; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2