title: Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes.
abstract: Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to 2-deoxyribose 5-phosphate with a molar yield of 55%.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. honda@bio.eng.osaka-u.ac.jp
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Honda
authors.forename: Shohei
authors.initials: S
authors.lastname: Maya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Omasa
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Hirota
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
mesh_terms: D032701:Biomimetics / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D005240:Feasibility Studies; D005632:Fructose / Q000737:chemistry*; D005818:Genetic Engineering / Q000379:methods*; D008190:Lyases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012267:Ribosemonophosphates / Q000138:chemical synthesis*; D015398:Signal Transduction / Q000502:physiology; D016963:Thermus thermophilus / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012267:Ribosemonophosphates; D005632:Fructose; C058245:2-deoxyribose 5-phosphate; D008190:Lyases