title: Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart.
abstract: A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.
authors.affiliation: Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. oujix@naramed-u.ac.jp
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.forename: Masahide
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Kei
authors.initials: K
authors.lastname: Moriya
authors.forename: Mariko
authors.initials: M
authors.lastname: Nishiofuku
authors.forename: Noriko
authors.initials: N
authors.lastname: Ouji-Sageshima
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Matsuda
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Nishimura
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ishizaka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017364:Collagenases / Q000008:administration & dosage*; D006352:Heart Ventricles / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology*; D051379:Mice
chemical_list: D017364:Collagenases