title: Comparison of serum versus plasma collection in gas chromatography--mass spectrometry-based metabolomics.
abstract: Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco ChromaTOF software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA- versus EDTA-ASA-plasma, EDTA-plasma versus serum and EDTA-ASA-plasma versus serum, respectively. To confirm that the observed signal intensities in the GC-TOF-MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC-MS using stable-isotope-labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA-plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.
authors.affiliation: Institute of Functional Genomics, University of Regensburg, Regensburg, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Dettmer
authors.forename: Martin F
authors.initials: MF
authors.lastname: Almstetter
authors.forename: Inka J
authors.initials: IJ
authors.lastname: Appel
authors.forename: Nadine
authors.initials: N
authors.lastname: Nürnberger
authors.forename: Gregor
authors.initials: G
authors.lastname: Schlamberger
authors.forename: Wolfram
authors.initials: W
authors.lastname: Gronwald
authors.forename: Heinrich H D
authors.initials: HH
authors.lastname: Meyer
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Oefner
mesh_terms: D000818:Animals; D002417:Cattle; D004492:Edetic Acid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010949:Plasma / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*
chemical_list: D004492:Edetic Acid