title: [Development and evaluation of whole-genome DNA microarray of Vibrio parahaemolyticus].
abstract: OBJECTIVE
  We developed and evaluated a whole-genome DNA microarray of Vibrio parahaemolyticus.
  
  
  METHODS
  Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results.
  
  
  RESULTS
  Microarray hybridization results were completely consistent with theory expectations and PCR verification results.
  
  
  CONCLUSIONS
  We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.
authors.affiliation: Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. shanlushanlu@163.com
authors.forename: Haihong
authors.initials: H
authors.lastname: Han
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Zeng
authors.forename: Shengjun
authors.initials: S
authors.lastname: Yin
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial