title: Mammalian cell transfection: the present and the future.
abstract: Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
authors.affiliation: Department of Pharmacology, University of Pennsylvania Medical School-Pharmacology, 36th and Hamilton Walk, Philadelphia, PA 19104, USA.
authors.forename: Tae Kyung
authors.initials: TK
authors.lastname: Kim
authors.forename: James H
authors.initials: JH
authors.lastname: Eberwine
mesh_terms: D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008322:Mammals / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods* / Q000639:trends*