title: Clinical significance of human kallikrein7 gene expression in colorectal cancer.
abstract: BACKGROUND
  The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.
  
  
  METHODS
  KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.
  
  
  RESULTS
  Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.
  
  
  CONCLUSIONS
  We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.
authors.affiliation: Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Mie, Japan. yasinoue@clin.medic.mie-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Inoue
authors.forename: Takehiko
authors.initials: T
authors.lastname: Yokobori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yokoe
authors.forename: Yuji
authors.initials: Y
authors.lastname: Toiyama
authors.forename: Chikao
authors.initials: C
authors.lastname: Miki
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Masato
authors.initials: M
authors.lastname: Kusunoki
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; C082472:KLK7 protein, human; D007610:Kallikreins