title: Antioxidative activities of histidine containing caffeic acid-dipeptides.
abstract: Antioxidants have been utilized in both the food and cosmetics industries to neutralize the activities of reactive oxygen species (ROS) and free radicals. Histidine-containing peptides are powerful antioxidants that exist in nature. Additionally, hydroxycinnamic acid (HCA)-peptide conjugates exhibit a synergistically enhanced antioxidative activity. Thus, caffeic acid (CA), a natural antioxidant, was conjugated to histidine-containing dipeptides (His dipeptides) in order to develop better antioxidants. The antioxidative activities were measured using 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test and lipid peroxidation test with ferric thiocyanate method. Some of the CA-His dipeptides exhibited better radical scavenging activities than CA, and all of the CA-His dipeptides showed enhanced lipid peroxidation inhibitory activities. His dipeptide enhanced the antioxidative activity of CA, and the position of histidine also affected the antioxidative activity of the compounds. CA-proline-histidine amide (CA-Pro-His-NH(2)) exhibited the highest activity in both the free radical scavenging test and the lipid peroxidation inhibition test.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Hyo-Suk
authors.initials: HS
authors.lastname: Seo
authors.forename: Seon-Yeong
authors.initials: SY
authors.lastname: Kwak
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002109:Caffeic Acids / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry / Q000494:pharmacology*; D006639:Histidine / Q000737:chemistry*; D015227:Lipid Peroxidation; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D004151:Dipeptides; D017382:Reactive Oxygen Species; D006639:Histidine; C040048:caffeic acid