Tobacco biomass hydrolysate enhances coenzyme Q10 production using photosynthetic Rhodospirillum rubrum.
Coenzyme Q10 (CoQ10), a potent antioxidative dietary supplement, was produced using a photosynthetic bacteria Rhodospirillum rubrum ATCC 25852 by submerged fermentation supplemented with tobacco biomass hydrolysate (TBH) in comparison with media supplemented with hydrolysates prepared with alfalfa (ABH) or spinach (SBH). Growth medium supplemented with 20% (v/v) TBH was found favorable with regard to cell density and CoQ10 concentration. The stimulation effects on cell growth (shortened lag phase, accelerated exponential growth, and elevated final cell concentration) and CoQ10 production (enhanced specific CoQ10 content per unit cell weight) could be attributed to the presence of solanesol, the precursor of CoQ10, in the tobacco biomass. The final yield of CoQ10 reached 20.16 mg/l in the fermentation medium supplemented with 20% TBH.
Tian
Yuting
Y
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, PR China.
Yue
Tianli
T
Yuan
Yahong
Y
Soma
Pavan K
PK
Williams
Patrick D
PD
Machado
Peter A
PA
Fu
Hong
H
Kratochvil
Robert J
RJ
Wei
Cheng-I
CI
Lo
Y Martin
YM
D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D010788:Photosynthesis; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D014451:Ubiquinone; C024989:coenzyme Q10