Genotype identification of human cystic echinococcosis in Isfahan, central Iran.
Echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of Iran with a major economic and public health importance. In the current study, Echinococcus granulosus isolates were collected from hospitalized patients in Isfahan, central Iran. The genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal DNA, followed by restriction fragment length polymorphism (RFLP) with two restriction enzymes namely AluI and MspI. As expected, each isolate yielded an approximately 1-kbp DNA fragment on the electrophoresis gel. According to RFLP results for both enzymes, all isolates had an equal pattern indicating the G1 genotype. Our findings confirmed that G1 is the dominant genotype of cystic echinococcosis in human in central Iran, with predilection to different organs including liver, lung, and brain, and warrants the importance of sheep dog cycle in public health.
Kia
Eshrat Bigom
EB
Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Rahimi
Hamidreza
H
Sharbatkhori
Mitra
M
Talebi
Ardeshir
A
Fasihi Harandi
Majid
M
Mirhendi
Hossein
H
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D019003:Deoxyribonuclease HpaII / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004443:Echinococcosis / Q000453:epidemiology* / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D019003:Deoxyribonuclease HpaII; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific