Inactivation of hnRNP K by expanded intronic AUUCU repeat induces apoptosis via translocation of PKCdelta to mitochondria in spinocerebellar ataxia 10.
We have identified a large expansion of an ATTCT repeat within intron 9 of ATXN10 on chromosome 22q13.31 as the genetic mutation of spinocerebellar ataxia type 10 (SCA10). Our subsequent studies indicated that neither a gain nor a loss of function of ataxin 10 is likely the major pathogenic mechanism of SCA10. Here, using SCA10 cells, and transfected cells and transgenic mouse brain expressing expanded intronic AUUCU repeats as disease models, we show evidence for a key pathogenic molecular mechanism of SCA10. First, we studied the fate of the mutant repeat RNA by in situ hybridization. A Cy3-(AGAAU)(10) riboprobe detected expanded AUUCU repeats aggregated in foci in SCA10 cells. Pull-down and co-immunoprecipitation data suggested that expanded AUUCU repeats within the spliced intronic sequence strongly bind to hnRNP K. Co-localization of hnRNP K and the AUUCU repeat aggregates in the transgenic mouse brain and transfected cells confirmed this interaction. To examine the impact of this interaction on hnRNP K function, we performed RT-PCR analysis of a splicing-regulatory target of hnRNP K, and found diminished hnRNP K activity in SCA10 cells. Cells expressing expanded AUUCU repeats underwent apoptosis, which accompanied massive translocation of PKCdelta to mitochondria and activation of caspase 3. Importantly, siRNA-mediated hnRNP K deficiency also caused the same apoptotic event in otherwise normal cells, and over-expression of hnRNP K rescued cells expressing expanded AUUCU repeats from apoptosis, suggesting that the loss of function of hnRNP K plays a key role in cell death of SCA10. These results suggest that the expanded AUUCU-repeat in the intronic RNA undergoes normal transcription and splicing, but causes apoptosis via an activation cascade involving a loss of hnRNP K activities, massive translocation of PKCdelta to mitochondria, and caspase 3 activation.
White
Misti C
MC
Department of Neurology, University of Texas Medical Branch, Galveston, Texas, USA.
Gao
Rui
R
Xu
Weidong
W
Mandal
Santi M
SM
Lim
Jung G
JG
Hazra
Tapas K
TK
Wakamiya
Maki
M
Edwards
Sharon F
SF
Raskin
Salmo
S
Teive
Hélio A G
HA
Zoghbi
Huda Y
HY
Sarkar
Partha S
PS
Ashizawa
Tetsuo
T
D017209:Apoptosis; D000067736:Ataxin-10; D002460:Cell Line; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000378:metabolism*; D006801:Humans; D007438:Introns; D008928:Mitochondria / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051745:Protein Kinase C-delta / Q000378:metabolism*; D021381:Protein Transport; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
C503889:ATXN10 protein, human; D000067736:Ataxin-10; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D009419:Nerve Tissue Proteins; D051745:Protein Kinase C-delta