title: USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories.
abstract: The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.
authors.affiliation: Department of Plant Biology and Biotechnology, and VKR Centre Pro-Active Plants, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Hussam H
authors.initials: HH
authors.lastname: Nour-Eldin
authors.forename: Fernando
authors.initials: F
authors.lastname: Geu-Flores
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Halkier
mesh_terms: D019677:Artificial Gene Fusion / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D007753:Laboratories; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA