Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival.
Various antioxidant strategies such as supplementation of antioxidants, limiting oxygen concentration with Oxyrase, and reducing reactive oxygen species through mild mitochondrial uncoupling had statistically significant beneficial effects on sperm cryopreservation from rhesus monkeys with low cryoresistant ejaculates. Individuals or species that have higher sensitivity to cryodamage may derive the most benefit from these treatments.
Dong
Qiaoxiang
Q
California National Primate Research Center, University of California, Davis, California 95616, USA.
Tollner
Theodore L
TL
Rodenburg
Sarah E
SE
Hill
Dana L
DL
VandeVoort
Catherine A
CA
D019297:2,4-Dinitrophenol / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods; D004542:Ejaculation / Q000502:physiology; D005615:Freezing; D008253:Macaca mulatta / Q000378:metabolism / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010105:Oxygenases / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D014475:Uncoupling Agents / Q000494:pharmacology
D000975:Antioxidants; D017382:Reactive Oxygen Species; D014475:Uncoupling Agents; D010105:Oxygenases; C072755:Oxyrase; D019297:2,4-Dinitrophenol