title: Salivary microbial counts and buffer capacity in children with acute lymphoblastic leukemia.
abstract: OBJECTIVE
  The aim of this study was to evaluate the caries activity in children undergoing maintenance stage chemotherapy courses.
  
  
  METHODS
  Forty-six children with acute lymphoblastic leukemia (ALL) were examined, the age ranged from 3 to 12 years with a mean age of 7(1/2) years. They were under maintenance stage chemotherapy at the Department of Pediatric Hematology of Chang-Gung Memorial Hospital, Taoyuan, Taiwan. A control group of healthy children was recruited by age and sex-matching criteria. The children received only a clinical dental examination without radiographs. Decayed (D), Missing (M), and Filled (F) Tooth surfaces (S) scores were recorded following the WHO criteria. After oral examination, stimulated saliva samples were collected from the subjects to exam the salivary Streptococcus mutans counts, salivary lactobacilli counts and salivary buffer capacity.
  
  
  RESULTS
  The Wilcoxon signed-rank test shows that the salivary Streptococcus mutans counts in ALL children were significantly lower than healthy subjects (P<.001) and lactobacilli counts were similar on both groups (P=.47). However, the ALL group tended to have lower salivary buffer capacity than the control group (P=.002). The mean DEFTS/DMFTS scores of the ALL group were higher than the control group, but the differences did not reach statistical significance.
  
  
  CONCLUSIONS
  Specific oral prevention regimens for ALL children undergoing chemotherapy should be planned for patients with unusually low salivary buffer capacity.
authors.affiliation: Department of Pediatric Dentistry, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan (Republic of China).
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Ou-Yang
authors.forename: Pei-Ching
authors.initials: PC
authors.lastname: Chang
authors.forename: Aileen I
authors.initials: AI
authors.lastname: Tsai
authors.forename: Tang-Her
authors.initials: TH
authors.lastname: Jaing
authors.forename: Shiao-Yu
authors.initials: SY
authors.lastname: Lin
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D004245:DMF Index; D003731:Dental Caries / Q000209:etiology / Q000382:microbiology / Q000517:prevention & control*; D003732:Dental Caries Activity Tests; D064420:Drug-Related Side Effects and Adverse Reactions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000302:isolation & purification; D016555:Matched-Pair Analysis; D009910:Oral Hygiene; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications / Q000188:drug therapy* / Q000382:microbiology / Q000503:physiopathology; D012016:Reference Values; D018570:Risk Assessment; D012463:Saliva / Q000382:microbiology / Q000502:physiology*; D013295:Streptococcus mutans / Q000302:isolation & purification