title: Extracorporeal support: improves donor renal graft function after cardiac death.
abstract: Donors after cardiac death (DCD) could increase the organ pool. Data supports good long-term renal graft survival. However, DCDs are <10% of deceased donors in the United States, due to delayed graft function, and primary nonfunction. These complications are minimized by extracorporeal support after cardiac death (ECS-DCD). This study assesses immediate and acute renal function from different donor types. DCDs kidneys were recovered by conventional rapid recovery or by ECS, and transplanted into nephrectomized healthy swine. Warm ischemia of 10 and 30 min were evaluated. Swine living donors were controls (LVD). ECS-DCDs were treated with 90 min of perfusion until organ recovery. After procurement, kidneys were cold storage 4-6 h. Renal vascular resistance (RVR), urine output (UO), urine protein concentration (UrPr) and creatinine clearance (CrCl), were collected during 4 h posttransplantation. All grafts functioned with adequate renal blood flow for 4 h. RVR at 4 h posttransplant returned to baseline only in the LVD group (0.36 mmHg/mL/min +/- 0.03). RVR was higher in all DCDs (0.66 mmHg/mL/min +/- 0.13), without differences between them. UO was >50 mL/h in all DCDs, except in DCD-30 (6.8 mL/h +/- 1.7). DCD-30 had lower CrCl (0.9 mL/min +/- 0.2) and higher UrPr >200 mg/dL, compared to other DCDs >10 mL/min and <160 mg/dL, respectively. Normothermic ECS can resuscitate kidneys to transplantable status after 30 min of cardiac arrest/WI.
authors.affiliation: General Surgery Department, Division of Transplantation, University of Michigan Health System, Ann Arbor, MI, USA. alvaror@umich.edu
authors.forename: A
authors.initials: A
authors.lastname: Rojas-Pena
authors.forename: J L
authors.initials: JL
authors.lastname: Reoma
authors.forename: E
authors.initials: E
authors.lastname: Krause
authors.forename: E L
authors.initials: EL
authors.lastname: Boothman
authors.forename: N P
authors.initials: NP
authors.lastname: Padiyar
authors.forename: K E
authors.initials: KE
authors.lastname: Cook
authors.forename: R H
authors.initials: RH
authors.lastname: Bartlett
authors.forename: J D
authors.initials: JD
authors.lastname: Punch
mesh_terms: D000818:Animals; D003404:Creatinine; D003643:Death; D051799:Delayed Graft Function / Q000503:physiopathology; D005260:Female; D006085:Graft Survival; D006323:Heart Arrest / Q000503:physiopathology; D007668:Kidney / Q000502:physiology / Q000503:physiopathology; D007677:Kidney Function Tests; D016030:Kidney Transplantation / Q000502:physiology*; D010477:Perfusion; D013552:Swine; D014019:Tissue Donors; D052096:Warm Ischemia
chemical_list: D003404:Creatinine