Lutein exposure, in ovo or in the diet, reduces parameters of inflammation in the liver and spleen laying-type chicks (Gallus gallus domesticus).
These trials examined whether the demonstrated effects of embryonic and dietary carotenoid exposure on the inflammatory immune response in fast growing chickens also occur in slow growing chickens. The systemic and local inflammatory responses of chicks were examined in two experiments with two in ovo lutein levels (C+, carotenoid replete; or C-, carotenoid-deplete), two dietary lutein levels (0 or 40 mg lutein/kg diet), and two inflammatory challenges [no exposure or lipopolysaccharide (LPS)-vaccinated]. At 24 h after LPS vaccination, spleen weight was not affected by diet or in ovo lutein, but liver weight increased from C+ eggs (p < 0.01), and in LPS-vaccinated chicks fed 0 mg lutein (p < 0.05), but not in chicks fed 40 mg lutein. Plasma carotenoids and liver carotenoids were reduced post-LPS (p < 0.05). Splenic IL-6 mRNA abundance was the greatest post-LPS in C- chicks fed 40 mg lutein vs. C+ chicks fed 40 mg lutein (p < 0.05). Hepatic IL-6, iNOS and TGFβ and splenic iNOS and TGFβ were not affected by in ovo or dietary lutein. The systemic and local inflammatory results are similar to those observed in fast growing chickens, and support that lutein-depleted birds have greater inflammatory responses.
Meriwether
L S
LS
Animal Science Department, California Polytechnic State University, San Luis Obispo, CA, USA.
Humphrey
B D
BD
Peterson
D G
DG
Klasing
K C
KC
Koutsos
E A
EA
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D004032:Diet / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007249:Inflammation / Q000517:prevention & control / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000187:drug effects; D014975:Lutein / Q000008:administration & dosage / Q000494:pharmacology*; D010063:Ovum; D013154:Spleen / Q000187:drug effects; D013158:Splenic Diseases / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*
D008070:Lipopolysaccharides; D014975:Lutein