title: Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effects of MeOH extract of PL (PLME) and its fractions on angiogenesis.
  
  
  METHODS
  PLME and its subsequent fractions (methylene chloride, ethyl acetate, n-butanol and aqueous fractions) were evaluated in vitro. Specifically, the anti-angiogenic activities of PLME and its fractions were investigated by measuring their effects on the proliferation, migration, tube formation and phosphorylation of vascular endothelial growth factor receptor (VEGFR)-2 in human umbilical vein endothelial cells (HUVECs). In addition, the in vivo Matrigel plug model was applied to evaluate new vessel formation.
  
  
  RESULTS
  The results revealed that PLME and its subsequent fractions, except for the aqueous fraction, led to significant inhibition of the proliferation, migration, tube formation and VEGFR-2 phosphorylation of HUVECs as well as in vivo angiogenesis.
  
  
  CONCLUSIONS
  These findings indicate the potential for the use of PLME in pathological situations involving stimulated angiogenesis, such as inflammation and tumor development.
authors.affiliation: Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 39 Hallym daehakgil, Chuncheon 200-702, Republic of Korea.
authors.forename: Yeon Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Shin
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000363:Agaricales; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D000432:Methanol / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D000084682:Phellinus; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D040301:Vascular Endothelial Growth Factor Receptor-2; D000432:Methanol