Fragmentation studies and electrospray ionization mass spectrometry of lapachol: protonated, deprotonated and cationized species.
Electrospray ionization mass spectrometric analysis of lapachol (2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone) was accomplished in order to elucidate the gas-phase dissociation reactions of this important biologically active natural product. The occurrence of protonated and cationized species in the positive mode and of deprotonated species in the negative mode was explored by means of collision-induced dissociation (CID) experiments. For the protonated molecule, the H(2)O and C(4)H(8) losses occur by two competitive channels. For the deprotonated molecule, the even-electron rule is not conserved, and the radicalar species are eliminated by formation of distonic anions. The fragmentation mechanism for each ion was suggested on the basis of computational thermochemistry. Atomic charges, relative energies, and frontier orbitals were employed aiming at a better understanding of the gas-phase reactivity of lapachol. Potential energy surfaces for fragmentation reactions were obtained by the B3LYP/6-31+G(d,p) model.
Vessecchi
Ricardo
R
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brasil.
Emery
Flavio S
FS
Galembeck
Sérgio E
SE
Lopes
Norberto P
NP
D008956:Models, Chemical; D009285:Naphthoquinones / Q000737:chemistry*; D044367:Phase Transition; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D009285:Naphthoquinones; C008252:lapachol