Correlation between surface hydrophilicity and osteoblastic differentiation on microgrooved titanium substrata.
Surface microgrooves and acid etching on titanium (Ti) have been proposed to enhance various cell behaviors. In this study, surface hydrophilicity, protein adsorption, and alkaline phosphatase activity of osteoblasts were analyzed and compared between microgrooved Ti, Ti with microgrooves and further acid etching, smooth Ti, and acid-etched smooth Ti. Correlations between the results of each experiment were analyzed using Pearson's correlation analysis, and the influential factor on alkaline phosphatase activity was determined using multiple stepwise regression analysis. Among groups, the Ti substrata with microgrooves and subsequent acid etching showed significantly greater surface hydrophilicity and alkaline phosphatase activity compared with smooth Ti, whereas the Ti substrata with only microgrooves showed the greatest protein adsorption. Multiple stepwise regression analysis determined the surface hydrophilicity of Ti as the influential factor on alkaline phosphatase activity. This study indicates that surface microgrooves and acid etching on Ti substrata enhance surface hydrophilicity, leading to increased alkaline phosphatase activity.
Lee
Suk-Won
SW
Department of Biomaterials & Prosthodontics, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Lee
Myung-Hyun
MH
Oh
Namsik
N
Park
Jung-Ae
JA
Leesungbok
Richard
R
Ahn
Su-Jin
SJ
D000134:Acid Etching, Dental / Q000379:methods; D000327:Adsorption; D000469:Alkaline Phosphatase / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003764:Dental Materials / Q000737:chemistry*; D003775:Dental Polishing / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
D003764:Dental Materials; D012709:Serum Albumin; D014025:Titanium; D000469:Alkaline Phosphatase