Direct detection of methicillin-resistant Staphylococcus aureus from blood cultures using an immunochromatographic immunoassay-based MRSA rapid kit for the detection of penicillin-binding protein 2a.
Using an EZ-Step MRSA rapid kit, a novel screening test for methicillin-resistant Staphylococcus aureus (MRSA) that detects penicillin-binding protein 2a, 34 of 36 MRSA-positive clinical blood culture samples were positive on direct testing (sensitivity, 94.4%), whereas 21 of 21 methicillin-susceptible S. aureus-positive samples were negative (specificity, 100%).
Shin
Kyeong Seob
KS
Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju 361-711, South Korea. ksshin@chungbuk.ac.kr [corrected]
Song
Hyung Geun
HG
Kim
Haejung
H
Yoon
Sangsun
S
Hong
Seung Bok
SB
Koo
Sun Hoe
SH
Kim
Jimyung
J
Kim
Jongwan
J
Roh
Kyoung Ho
KH
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001426:Bacterial Proteins / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D046915:Penicillin-Binding Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins