Functional replacement of yeast flavocytochrome b(2) with bacterial L-lactate dehydrogenase.
Using yeast genetic complementation, we show here that Rhodobacter sphaeroidesl-lactate dehydrogenase can functionally replace flavocytochrome b(2) in Saccharomyces cerevisiae, only when a matrix-targeting signal of flavocytochrome b(2) is fused with the bacterial enzyme. The recombinant l-lactate dehydrogenase may add alternative route of mitochondrial electron transport other than flavocytochrome b(2).
Sakai
Hiroaki
H
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Nagahama
Kazuhiro
K
Taguchi
Hisataka
H
Akamatsu
Takashi
T
Morimura
Shigeru
S
Kida
Kenji
K
Matsuoka
Masayoshi
M
D004926:Escherichia coli / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D042844:L-Lactate Dehydrogenase (Cytochrome) / Q000502:physiology*; D019344:Lactic Acid / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology
D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D042844:L-Lactate Dehydrogenase (Cytochrome)