The role of enoyl-CoA hydratase short chain 1 and peroxiredoxin 3 in PP2-induced apoptosis in human breast cancer MCF-7 cells.
We show that 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) induces apoptosis and down-regulates the expression of enoyl-CoA hydratase short chain 1 (ECHS1) and peroxiredoxin 3 (PRDX3) in human breast cancer MCF-7 cells. The decrease of ECHS1 and PRDX3 was validated by Western blot and quantitative real-time reverse transcription-PCR in MCF-7 and other carcinoma cells. Knockdown and over-expression of ECHS1 and/or PRDX3 further supported the key role of ECHS1 and PRDX3 in regulation of PP2-induced apoptosis. These results suggest a possible apoptotic pathway whereby down-regulation of ECHS1 and PRDX3 potentiates PP2-induced apoptosis in MCF-7 cells.
Liu
Xiang
X
College of Life Sciences, Peking University, Beijing, PR China.
Feng
Renqing
R
Du
Liying
L
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002477:Cells / Q000378:metabolism / Q000473:pathology; D022082:Cellular Structures / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation / Q000187:drug effects; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D054464:Peroxiredoxins
D054464:Peroxiredoxins; D004746:Enoyl-CoA Hydratase