An on-chip whole blood/plasma separator with bead-packed microchannel on COC polymer.
A disposable on-chip whole blood/plasma separator, which is able to separate plasma from whole human blood by capillary force through a bead-packed microchannel, has been designed, fabricated and characterized in this work. Various sizes of silica beads were slurry-packed through a microchannel using a bump structure which held beads in a defined region. The bead-packed microchannel induces a capillary force which allows plasma to move forward through the bead-packed column more rapidly than red blood cells (RBCs). The blood/plasma separator with bead-packed microchannel has successfully separated plasma from the whole blood without haemolysis of RBCs. The separation method developed in this work can be applied to various on-chip stationary filtrations of RBC for point-of-care clinical diagnostics.
Shim
Joon S
JS
Microsystems and BioMEMS Laboratory, Department of Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
Browne
Andrew W
AW
Ahn
Chong H
CH
D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods*; D003516:Cycloparaffins / Q000737:chemistry*; D006801:Humans; D046208:Microchip Analytical Procedures / Q000379:methods*; D008863:Microspheres; D009068:Movement; D010316:Particle Size; D010949:Plasma; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
D003516:Cycloparaffins; D011108:Polymers; D012822:Silicon Dioxide