title: Inhibitory effects of fatty acids on the activity of mushroom tyrosinase.
abstract: The effects of fatty acids, octanoic acid, (2E, 4E)-hexa-2,4-dienoic acid, hexanoic acid, (2E)-but-2-enoic acid, and butyric acid on the activities of mushroom tyrosinase have been investigated. The results showed that the fatty acids can potently inhibit both monophenolase activity and diphenolase activity of tyrosinase, and that the unsaturated fatty acids exhibited stronger inhibitory effect against tyrosinase than the corresponding saturated fatty acids, and the inhibitory effects were enhanced with the extendability of the fatty acid chain. For the monophenolase activity, the fatty acids could not only lengthen the lag period, but also decrease the steady-state activities. For the diphenolase activity, fatty acids displayed reversible inhibition. Kinetic analyses showed that octanoic acid and hexanoic acid were mixed-type inhibitors and (2E,4E)-hexa-2,4-dienoic acid and (2E)-but-2-enoic acid were noncompetitive inhibitors. The inhibition constants have been determined and compared.
authors.affiliation: Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Yun-Ji
authors.initials: YJ
authors.lastname: Guo
authors.forename: Zhi-Zhen
authors.initials: ZZ
authors.lastname: Pan
authors.forename: Chao-Qi
authors.initials: CQ
authors.lastname: Chen
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Hu
authors.forename: Feng-Jiao
authors.initials: FJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Jiang-Hua
authors.initials: JH
authors.lastname: Yan
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D007700:Kinetics; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D005227:Fatty Acids; D005656:Fungal Proteins; D014442:Monophenol Monooxygenase