title: Evaluation of a bovine rotavirus VP6 vaccine efficacy in the calf model of infection and disease.
abstract: Group A bovine rotavirus (BRV) is the major cause of acute viral gastroenteritis in neonatal calves worldwide. Due to the early susceptibility to the infection prevention strategies are based on the improvement of passive immunity levels through cow vaccination in the last third of gestation. The major capsid antigen (VP6) of BRV is the most immunogenic viral protein and it is highly conserved among group A BRV. In this work, VP6 protein from BRV C-486 strain (P[1]G6) was expressed in insect cells using the baculovirus expression vector system. Recombinant VP6 was used to immunize cows and vaccine's efficacy was assessed in a colostrum-deprived calf model of BRV infection and disease. Immune colostrum pool was generated using first and second milking of the immunized cows. Calves receiving one dose of immune colostrum within the first 6h of life, or colostrum-deprived calves were orally inoculated with virulent BRV at 2 days of age. The animals were monitored for diarrhea, virus shedding and isotype-specific antibodies responses to BRV in both feces and serum. Calves receiving VP6-immune colostrum showed a reduction of both diarrhea and virus shedding (in terms of viral titer and excretion period) in comparison with the colostrum-deprived calves.
authors.affiliation: Instituto de Virología, CICVyA, INTA, Las Cabañas y de los Reseros s/n, Castelar, 1686 Hurlingham, Buenos Aires, Argentina.
authors.forename: Diego Daniel
authors.initials: DD
authors.lastname: Gonzalez
authors.forename: Marina Valeria
authors.initials: MV
authors.lastname: Mozgovoj
authors.forename: Demian
authors.initials: D
authors.lastname: Bellido
authors.forename: Daniela Vanesa
authors.initials: DV
authors.lastname: Rodriguez
authors.forename: Fernando María
authors.initials: FM
authors.lastname: Fernandez
authors.forename: Andrés
authors.initials: A
authors.lastname: Wigdorovitz
authors.forename: Viviana Gladys
authors.initials: VG
authors.lastname: Parreño
authors.forename: María José
authors.initials: MJ
authors.lastname: Dus Santos
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003126:Colostrum / Q000276:immunology; D003967:Diarrhea / Q000517:prevention & control / Q000662:veterinary; D005243:Feces / Q000821:virology; D005260:Female; D007114:Immunization; D012400:Rotavirus Infections / Q000517:prevention & control / Q000662:veterinary*; D022243:Rotavirus Vaccines / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D022243:Rotavirus Vaccines; C061332:VP6 protein, Rotavirus