title: Bioluminescence imaging of dual gene expression at the single-cell level.
abstract: Bioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.
authors.affiliation: Regenerative Medicine/Tissue Engineering Division, Research Center for Cooperative Projects, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Hyuckjoon
authors.initials: H
authors.lastname: Kwon
authors.forename: Toshiteru
authors.initials: T
authors.lastname: Enomoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shimogawara
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yasuda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000235:genetics; D014162:Transfection
chemical_list: D011993:Recombinant Fusion Proteins; D008156:Luciferases