[The immunogenicity of mouse neural stem cells].
OBJECTIVE
To explore the immunogenicity of mouse neural stem cells (NSCs), thus help to solve the problem of immunologic rejection in NSCs transplantation.


METHODS
(1) Thirty C57BL/6 mice were assigned randomly into two groups, then the two groups were immuned by intraperitoneal injection respectively with BALB/c mice's NSCs or hepatic cells (as control). Afterwards one-way mixed lymphocyte culture was performed with BALB/c mice's NSCs or hepatic cells and immuned C57BL/6 mice's T lymphocytes. Immune response of T lymphocyte was detected by liquid scintillation counter. (2) The MHC-I and MHC-II molecules of BALB/c mice NSCs were stained with FITC- and PE-labeled antibody respectively. Then the expression of MHC-I and MHC-II were detected by flow cytometry.


RESULTS
As shown by liquid scintillation counter, the cpm value of NSCs was 16592.8 +/- 2865.3, and that of the hepatic cells was 27815.0 +/- 2416.3 (P<0.01). About (87.55 +/- 3.65)% NSCs expressed neither MHC-I nor MHC-II, but there were only about (27.45 +/- 1.86)% hepatocytes showed both MHC-I and MYC-II negative (P<0.01).


CONCLUSIONS
The mouse NSCs show weaker immunogenicity and their immunogenicity is significantly weaker than their hepatocytes.
Gao
Feng
F
Department of neurosurgery, Second Affiliated Hospital, Zhejiang University, Hangzhou, China. samgf@163.com
Yang
Xiao-feng
XF
Zheng
Xue-sheng
XS
Shen
Gang
G
Liu
Wei-guo
WG
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology; D006084:Graft Rejection; D006649:Histocompatibility Antigens / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000276:immunology; D051379:Mice; D009420:Nervous System / Q000166:cytology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
D006649:Histocompatibility Antigens