Acetyl-L-carnitine protects yeast cells from apoptosis and aging and inhibits mitochondrial fission.
In this work we report that carnitines, in particular acetyl-l-carnitine (ALC), are able to prolong the chronological aging of yeast cells during the stationary phase. Lifespan extension is significantly reduced in yca1 mutants as well in rho(0) strains, suggesting that the protective effects pass through the Yca1 caspase and mitochondrial functions. ALC can also prevent apoptosis in pro-apoptotic mutants, pointing to the importance of mitochondrial functions in regulating yeast apoptosis and aging. We also demonstrate that ALC attenuates mitochondrial fission in aged yeast cells, indicating a correlation between its protective effect and this process. Our findings suggest that ALC, used as therapeutic for stroke, myocardial infarction and neurodegenerative diseases, besides the well-known anti-oxidant effects, might exert protective effects also acting on mitochondrial morphology.
Palermo
Vanessa
V
Department of Cell and Developmental Biology, University of Rome, Rome, Italy.
Falcone
Claudio
C
Calvani
Menotti
M
Mazzoni
Cristina
C
D019342:Acetic Acid / Q000494:pharmacology; D000108:Acetylcarnitine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002110:Caffeine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D010641:Phenotype; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013997:Time Factors
C093543:LSM4 protein, S cerevisiae; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D002110:Caffeine; D000108:Acetylcarnitine; D006861:Hydrogen Peroxide; D019342:Acetic Acid