A rapid and specific derivatization procedure to identify acyl-glucuronides by mass spectrometry.
A simple procedure is described to identify acyl-glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl-glucuronide from an extract. Glucuronides derived from carbamic acids, and alkyl- and aromatic amines, are inert to the derivatization reaction conditions, making the hydroxamic acid derivative a fingerprint for acyl-glucuronides.
Vaz
Alfin D N
AD
Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer Global Research and Development, Groton, CT 06340, USA. alfin.vaz@pfizer.com
Wang
Wei Wei
WW
Bessire
Andrew J
AJ
Sharma
Raman
R
Hagen
Anne E
AE
D002219:Carbamates / Q000737:chemistry; D020719:Glucuronides / Q000737:chemistry*; D006877:Hydroxamic Acids / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*
D002219:Carbamates; D020719:Glucuronides; D006877:Hydroxamic Acids; D019811:Hydroxylamine; C070766:carbamic acid