title: Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death.
abstract: Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.
authors.affiliation: University Women's Hospital, Department of Biomedicine, Basel University Hospital, Basel, Switzerland.
authors.forename: Anurag Kumar
authors.initials: AK
authors.lastname: Gupta
authors.forename: Manjunath B
authors.initials: MB
authors.lastname: Joshi
authors.forename: Maria
authors.initials: M
authors.lastname: Philippova
authors.forename: Paul
authors.initials: P
authors.lastname: Erne
authors.forename: Paul
authors.initials: P
authors.lastname: Hasler
authors.forename: Sinuhe
authors.initials: S
authors.lastname: Hahn
authors.forename: Therese J
authors.initials: TJ
authors.lastname: Resink
mesh_terms: D016923:Cell Death / Q000187:drug effects / Q000276:immunology; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology; D004727:Endothelium / Q000187:drug effects / Q000276:immunology; D005110:Extracellular Space / Q000187:drug effects / Q000276:immunology*; D005260:Female; D056660:Hereditary Autoinflammatory Diseases; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000502:physiology; D011225:Pre-Eclampsia / Q000276:immunology; D011247:Pregnancy
chemical_list: D016209:Interleukin-8