Immunohistochemical analysis of Metadherin in proliferative and cancerous breast tissue.
BACKGROUND
Metadherin (MTDH) has been reported to be associated with cancer progression in various types of human cancers including breast cancer. Whether MTDH contributes to carcinogenesis of breast cancer is still unknown. In the present study, we investigated the expression of MTDH in normal, UDH (usual ductal hyperplasia), ADH (atypical ductal hyperplasia), DCIS (ductal carcinoma in situ) and invasive cancer to explore the possible role of MTDH for breast cancer carcinogenesis.


METHODS
Immunohistochemistry was employed on paraffin sections of surgical removed breast samples.


RESULTS
The immunohistochemical results showed almost no staining in normal tissue, moderate staining in ADH and UDH, intense MTDH stains in DCIS and cancer. Statistical analysis demonstrated significant different MTDH expression between proliferative and cancerous breast lesions (p < 0.001). MTDH was positively correlated with the histological differentiation of DCIS (p = 0.028). In breast cancer, statistical analysis revealed a significant correlation between MTDH expression with patients' age (p = 0.042), ER status (p = 0.018) and p53 status (p = 0.001). We also examined the effect of MTDH on cell proliferation in DCIS and cancer, and we found that MTDH overexpression was significantly correlated with high Ki67 index (p = 0.008 and p = 0.036, respectively).


CONCLUSIONS
MTDH overexpression could be identified in proliferative breast lesions and may contribute to breast cancer progression.
Su
Peng
P
Department of Pathology, Qilu Hospital, Shandong University School of Medicine, Ji'nan, China.
Zhang
Qinghui
Q
Yang
Qifeng
Q
D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000473:pathology; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000032:analysis*; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008565:Membrane Proteins; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016612:Paraffin Embedding; D016601:RNA-Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53; C483039:MTDH protein, human; C495901:TP53 protein, human; C508053:ERBB2 protein, human