title: Evaluation of genetic and functional diversity of Stenotrophomonas isolates from diverse effluent treatment plants.
abstract: In this study, the samples were collected from nine ETPs and soil contaminated with petroleum products. The genetic diversity of 30 Stenotrophomonas isolates was demonstrated by phylogenetic analysis of their 16S rRNA gene nucleotide sequences, and randomly amplified polymorphic DNA (RAPD) analysis supplemented with in silico signature and restriction enzyme (REs--AluI, BfaI, DpnII, HaeIII, RsaI and Tru9I) digestion analyses. Genetic diversity based on nucleotide sequence data revealed distinct clusters. Functional diversity was analysed on the basis of the abilities of these isolates to degrade phenol, p-cresol, catechol, 4-methylcatechol and hydroquinone. Based on the environmental, genetic and functional diversities, a consortium of mixed defined microbes has been proposed for bioremediation programs.
authors.affiliation: Environmental Genomics Unit, National Environmental Engineering Research Institute-NEERI, CSIR, Nehru Marg, Nagpur 440020-MH, India.
authors.forename: Vinita
authors.initials: V
authors.lastname: Verma
authors.forename: Sajan C
authors.initials: SC
authors.lastname: Raju
authors.forename: Atya
authors.initials: A
authors.lastname: Kapley
authors.forename: Vipin Chandra
authors.initials: VC
authors.lastname: Kalia
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
mesh_terms: D001673:Biodegradation, Environmental; D005798:Genes, Bacterial; D014644:Genetic Variation; D010802:Phylogeny; D020588:Stenotrophomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D062065:Wastewater
chemical_list: D062065:Wastewater