pCEC coupling with ESI-MS for the analysis of beta(2)-agonists and narcotics using a poly-(1-hexadecene-co-TMPTMA) monolithic column.
A pressure-assisted CEC with ESI-MS based on poly(1-hexadecene-co-trimethylolpropane trimethacrylate) monolithic column for rapid analysis of two beta(2)-agonists and three narcotics was established in this article. After the organic polymer-based monolithic column was prepared by an in-situ polymerization procedure, a systematic investigation of the pressure-assisted CEC separation and ESI-MS detection parameters was performed. Baseline separation of the studied analytes could be obtained using the solution containing 75% ACN v/v and 20 mmol/L ammonium acetate with pH 8.0 as running buffer, when applying separation voltage of 20 kV and assisted pressure of 5 bar. Under the optimized conditions, two beta(2)-agonists and three narcotics could be completely resolved and accurately determined within 15 min. Finally, the proposed method was successfully used for real urine samples detection.
Cheng
Jintian
J
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, The Sport Science Research Center, Fuzhou University, Fuzhou, P. R. China.
Zhang
Lan
L
Lu
Qiaomei
Q
Lu
Minghua
M
Chen
Zongbao
Z
Chen
Guonan
G
D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D000420:Albuterol / Q000737:chemistry / Q000652:urine; D000475:Alkenes / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008297:Male; D008689:Methacrylates / Q000737:chemistry; D009294:Narcotics / Q000032:analysis* / Q000737:chemistry; D011312:Pressure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000085:Acetates; D000097:Acetonitriles; D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D000475:Alkenes; D008689:Methacrylates; D009294:Narcotics; D000420:Albuterol; C018824:ammonium acetate; C028643:trimethylolpropane trimethacrylate; C032159:acetonitrile