Influence of intense pulsed light on the secretion of TGF-beta1 in cultured human fibroblasts and intervention of JNK inhibitor.
OBJECTIVE
To determine the influence of intense pulsed light (IPL) on the secretion of TGF-beta1 in cultured human fibroblasts and the intervention of JNK inhibitor.


METHODS
The callan foreskin fibroblasts were cultured and divided into 2 groups. In the IPL treatment group, cells were irradiated with IPL with fluences of 0 (negative control), 10, 18, 27, 36, and 36 J/cm2*2 (irradiated with IPL with fluences of 36 J/cm2 twice). In the IPL+inhibitor group, cells were irradiated with IPL with fluences of 36 J/cm2 after incubation with the inhibitor SP600125 for 2 h. TGF-beta1 in the culture supernatant was evaluated 48 h after the irradiation using enzyme-linked immunosorbent assay.


RESULTS
Compared with the negative control, TGF-beta1 in the culture supernatant decreased at the IPL irradiation of 10, 18, 27, and 36 J/cm2, whereas TGF-beta1 increased at the IPL irradiation of 36 J/cm2*2. In the IPL+inhibitor group, the concentration of TGF-beta1 in the culture supernatant decreased compared with the controls (P<0.05).


CONCLUSIONS
IPL can suppress the secretion of TGF-beta1 at the lower fluence and promote the secretion at a higher fluence. JNK inhibitor may play an inhibitive role when IPL regulates the TGF-beta1 secretion in cultured human fibroblasts. IPL may stimulate TGF-beta1 secretion of the fibroblast cells in human skin via JNK signal pathway.
Lu
Jianyun
J
Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, China. kateljy@yahoo.com.cn
Yu
Xiaofeng
X
Kang
Jian
J
Ouyang
Zehui
Z
Geng
Xuerui
X
Xiang
Yaping
Y
Huang
Jinhua
J
D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D008027:Light; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors*; D008297:Male; D012867:Skin / Q000166:cytology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D055815:Young Adult
D053773:Transforming Growth Factor beta1; D048670:MAP Kinase Kinase 4