Optimization of the production of organic solvent-stable protease by Bacillus sphaericus DS11 with response surface methodology.
Response surface methodology (RSM) was employed to enhance the production of organic solvent-stable protease by Bacillus sphaericus DS11. A significant influence of glycerol, MgSO₄·7H₂O, and pH on organic solvent-stable protease production was noted with Plackett-Burman design. Then, a three-level Box-Behnken design was employed to optimize the medium composition and culture conditions for the production of the protease in shake-flask. Using this methodology, the quadratic regression model of producing organic solvent-stable protease was built and the optimal combinations of media constituents and culture conditions for maximum protease production were determined as glycerol 12.47 g/L, MgSO₄·7H₂O 0.73 g/L, and pH 8.25. Protease production obtained experimentally coincident with the predicted value and the model was proven to be adequate. The enhancement of protease from 465.06 U/mL to 1182.68 U/mL was achieved with the optimization procedure.
Liu
Shu
S
School of Marine Science and Technology, Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang , Jiangsu Province 222005, China.
Fang
Yaowei
Y
Lv
Mingsheng
M
Wang
Shujun
S
Chen
Li
L
D001407:Bacillus / Q000201:enzymology*; D002244:Carbon / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D013499:Surface Properties
D002244:Carbon; D010447:Peptide Hydrolases; D009584:Nitrogen