[RRLC-UV-MS fingerprint of Radix Astragali].
OBJECTIVE
To establish a method for detecting the fingerprint of Radix Astragali by RRLC-UV-MS.


METHODS
Separation was performed on an Agilent Zorbax Extend C18 column (2.1 mm x 100 mm, 1.8 microm). Gradient elution was performed by the mobile phases consisting of acetonitrile and 0.1% formic acid with the flow rate of 0.3 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was 30 degrees C.


RESULTS
The method of fingerprint analysis on Radix Astragali by RRLC-UV-MS was established. Eleven samples of Radix Astragali were analyzed, the similarities were over 0.92, and seven peaks in the fingerprint were designated.


CONCLUSIONS
The method can be applied to the quality control and studies on chemical constituents of Radix Astragali.
Su
Xian
X
Department of Traditional Chinese Medicine & Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wu
Lijun
L
Tu
Pengfei
P
D027883:Astragalus Plant / Q000737:chemistry*; D001565:Benzoates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D001565:Benzoates; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts