title: Porcine circovirus type 2 (PCV2) Cap and Rep proteins are involved in the development of cell-mediated immunity upon PCV2 infection.
abstract: The aim of the present study was to investigate the role of the capside (Cap) and replicase (Rep) proteins of Porcine circovirus type 2 (PCV2) as well as the whole PCV2 (both PCV2a and PCV2b genotypes) in the induction of cell-mediated immunity upon infection. At 6 weeks of age, six pigs were intranasally inoculated with the Stoon 1010 (Stoon) isolate (PCV2a) and seven with the Sp-7-10-54-13 (Sp) isolate (PCV2b). None of the pigs developed clinical disease but the Sp group had significantly higher proportion of pigs with PCV2-associated lesions and PCV2 load in tissues compared to the Stoon group. In both groups, development of IFN-gamma secreting cells (SC) in response to the whole PCV2 and Cap protein was detected by means of an ELISPOT from day 7 post-inoculation (PI) to the end of the study (21 days PI). Significant responses against Rep protein were only detected in Sp-inoculated pigs. No differences in ELISPOT results were seen when either PCV2a or PCV2b was used in vitro to recall peripheral blood mononuclear cells (PBMC) in any group. Stimulation of PBMC with the whole virus but not with Cap or Rep protein induced IL-10-SC in all pigs regardless of their PCV2 infection status, indicating an innate origin of this response. The results from this study demonstrate that PCV2-infected pigs developed cell-mediated immunity to Cap and Rep proteins and that, in the course of a sub-clinical infection, development and strength of such responses are possibly related to the levels of PCV2 replication.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Fort
authors.forename: Marina
authors.initials: M
authors.lastname: Sibila
authors.forename: Miquel
authors.initials: M
authors.lastname: Nofrarías
authors.forename: Eva
authors.initials: E
authors.lastname: Pérez-Martín
authors.forename: Alex
authors.initials: A
authors.lastname: Olvera
authors.forename: Enric
authors.initials: E
authors.lastname: Mateu
authors.forename: Joaquim
authors.initials: J
authors.lastname: Segalés
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000276:immunology*; D018173:Circoviridae Infections / Q000473:pathology / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics; D058501:Enzyme-Linked Immunospot Assay; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D012324:RNA-Dependent RNA Polymerase / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000473:pathology; D014766:Viremia / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D016753:Interleukin-10; D007371:Interferon-gamma; D012324:RNA-Dependent RNA Polymerase