Mapping of the 5' terminal nucleotides of Grapevine leafroll-associated virus 3 sgRNAs.
Grapevine leafroll-associated virus 3 (GLRaV-3) is a positive sense, single stranded RNA virus that has a detrimental effect on wine and table grapes worldwide. Previous studies have shown that GLRaV-3, like other closteroviruses produces subgenomic (sg) RNAs during replication and that these sgRNAs are deployed for the expression of the ORFs on the 3' half of the genome. In this study a dsRNA blot confirmed the presence of three, 3' co-terminal sgRNAs species [sgRNA(ORF3/4), sgRNA(ORF5) and sgRNA(ORF6)] in GLRaV-3-infected plant material when using a probe directed at the coat protein. The specific 5' terminal nucleotides for these sgRNAs as well as four additional sgRNAs [sgRNA(ORF7), sgRNA(ORF8), sgRNA(ORF9) and sgRNA(ORF10-12)] were determined by RLM-RACE for GLRaV-3 isolate GP18.
Maree
Hans J
HJ
Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Gardner
Helena F J
HF
Freeborough
Michael-John
MJ
Burger
Johan T
JT
D029381:Closteroviridae / Q000235:genetics* / Q000254:growth & development*; D015967:Gene Expression Regulation, Viral; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins