[Using HPLC-MS/MS method to analyze monohydroxy metabolites of PAHs in urine].
OBJECTIVE
To establish a HPLC-MS/MS method for the simultaneous determination of 4 monohydroxy metabolites of polycyclic aromatic hydrocarbons (PAHs) in urine, including 1-naphthol, 2-naphthol, 3-phenanthrol and 1-pyrenol).


METHODS
After being treated by enzymatic hydrolysis, urine samples were purified by liquid-liquid extraction, evaporated under nitrogen to constant volume and examined by HPLC-MS/MS. Target compounds were quantified by internal standard calibration curve.


RESULTS
The reproducibility and precision were improved by internal standard calibration. The recovery of four target compounds were in the range of 89.7%-103.0%. The precision of intra- and inter-batch was 1.0%-4.2% and 1.8%-5.8% respectively. The limits of quantification was 0.2 microg/L for 1-naphthol, 2-naphthol and 1-pyrenol and the limits of quantification was 0.1 microg/L for 3-phenanthrol.


CONCLUSIONS
The sensitivity, reproducibility and recovery of HPLC-MS/MS method were good for the analysis of 1-naphthol, 2-naphthol, 3-phenanthrol and 1-pyrenol in the urine of people on occupational and nonoccupational exposure to PAHs.
Huang
Chuanfeng
C
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. chuanfengh@yahoo.com.cn
Yan
Huifang
H
Pan
Zufei
Z
Dai
Yufei
Y
Niu
Yong
Y
Wang
Yujiang
Y
Zheng
Yuxin
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004781:Environmental Exposure / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D009284:Naphthols / Q000652:urine*; D011721:Pyrenes / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
D009284:Naphthols; D011721:Pyrenes; C029350:1-naphthol; C033146:1-hydroxypyrene; C028405:2-naphthol