IEF pattern classification-derived criteria for the identification of epoetin-delta in urine.
Epoetin-delta (Dynepo Shire Pharmaceuticals, Basing stoke, UK) is a synthetic form of erythropoietin (EPO) whose resemblance with endogenous EPO makes it hard to identify using the classical identification criteria. Urine samples collected from six healthy volunteers treated with epoetin-delta injections and from a control population were immuno-purified and analyzed with the usual IEF method. On the basis of the EPO profiles integration, a linear multivariate model was computed for discriminant analysis. For each sample, a pattern classification algorithm returned a bands distribution and intensity score (bands intensity score) saying how representative this sample is of one of the two classes, positive or negative. Effort profiles were also integrated in the model. The method yielded a good sensitivity versus specificity relation and was used to determine the detection window of the molecule following multiple injections. The bands intensity score, which can be generalized to epoetin-alpha and epoetin-beta, is proposed as an alternative criterion and a supplementary evidence for the identification of EPO abuse.
Lamon
Séverine
S
Laboratoire Suisse d'Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Epalinges, Switzerland.
Boccard
Julien
J
Sottas
Pierre-Edouard
PE
Glatz
Nicolas
N
Wuerzner
Grégoire
G
Robinson
Neil
N
Saugy
Martial
M
D000328:Adult; D000465:Algorithms; D003663:Decision Trees; D016002:Discriminant Analysis; D004300:Doping in Sports; D004921:Erythropoietin / Q000652:urine*; D006077:Government Agencies; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D008297:Male; D015999:Multivariate Analysis; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity
D011994:Recombinant Proteins; D004921:Erythropoietin; C516023:epoetin delta