title: Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting.
abstract: We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the electrode surface. The electrical field distribution is calculated in different electrode configurations by numerical simulations. Cell sorting shows high lysis efficiency, 99% of yeast cells sorted after lysis featuring dielectric properties similar to dead cells. A study of the potential device throughput is performed.
authors.affiliation: Laboratoire de Microsystèmes LMIS4, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015, Lausanne, Switzerland. guillaume.mernier@epfl.ch
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mernier
authors.forename: Niccolò
authors.initials: N
authors.lastname: Piacentini
authors.forename: Thomas
authors.initials: T
authors.lastname: Braschler
authors.forename: Nicolas
authors.initials: N
authors.lastname: Demierre
authors.forename: Philippe
authors.initials: P
authors.lastname: Renaud
mesh_terms: D002452:Cell Count; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003198:Computer Simulation; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D056656:Lab-On-A-Chip Devices; D012441:Saccharomyces cerevisiae / Q000166:cytology