In vitro self-assembly of gold nanoparticle-coated poly(3-hydroxybutyrate) granules exhibiting plasmon-induced thermo-optical enhancements.
Polyhydroxyalkanoate (PHA) synthase attached to gold nanoparticles (AuNP) produce poly(3-hydroxybutyrate) (PHB) upon the addition of 3-hydroxybutyrate-CoA, and then coalesce to form micrometer-sized AuNP-coated PHB granules. These AuNP-coated PHB granules are potential theranostic agents that have enhanced imaging capabilities and are capable of heating upon near-infrared laser irradiation. The AuNP-coated PHB exhibited 11-fold enhancement in surface-enhanced Raman scattering over particles prior polymerization. Stained AuNP-coated PHB exhibited a 6-fold enhancement in fluorescence intensity as well as a 1.3-fold decrease in photobleaching rate compared to PHB granules alone. The granules were also shown to emit heat when illuminated at 808 nm with a 3.9-fold increase in heating rate compared to particles alone.
Rey
Diego A
DA
Strickland
Aaron D
AD
Kirui
Dickson
D
Niamsiri
Nuttawee
N
Batt
Carl A
CA
D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006358:Hot Temperature; D006885:Hydroxybutyrates / Q000737:chemistry*; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry*; D055095:Optics and Photonics / Q000379:methods; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D000094122:Polyhydroxybutyrates
D006046:Gold; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates