title: Accurate non-invasive image-based cytotoxicity assays for cultured cells.
abstract: BACKGROUND
  The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.
  
  
  RESULTS
  Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.
  
  
  CONCLUSIONS
  This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
authors.forename: Patricia
authors.initials: P
authors.lastname: Marqués-Gallego
authors.forename: Hans
authors.initials: H
authors.lastname: den Dulk
authors.forename: Claude
authors.initials: C
authors.lastname: Backendorf
authors.forename: Jaap
authors.initials: J
authors.lastname: Brouwer
authors.forename: Jan
authors.initials: J
authors.lastname: Reedijk
authors.forename: Julian F
authors.initials: JF
authors.lastname: Burke
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002945:Cisplatin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D051379:Mice; D008853:Microscopy; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D002945:Cisplatin