Screening and characterization of an inhibitory chemical specific to Arabidopsis gibberellin 2-oxidases.
The hydroxylation of gibberellin (GA) at the 2-position is known as the major cause of inactivation of GAs, whose reaction is catalyzed by 2-oxoglutarate dependent dioxygenases, also termed GA 2-oxidases (GA2oxs). To block GA catabolism in plants, a few chemicals can be used. To obtain novel inhibitors specific to GA2oxs, we performed in vitro random screenings by using (3)H-16,17-dihydro-GA(4) and recombinant Arabidopsis GA2ox2. As a result, one candidate, methyl 6-chloro-3H-1,2,3-benzodithiazole-4-carboxylate 2-oxide (CBTC), was selected from the screening, and was subjected to in-planta evaluations. CBTC promoted both the germination and elongation of Arabidopsis seedlings. This strongly suggests that CBTC inhibits GA2oxs in Arabidopsis with high specificity.
Otani
Masato
M
Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yoon
Jung-Min
JM
Park
Seung-Hyun
SH
Asami
Tadao
T
Nakajima
Masatoshi
M
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D006899:Mixed Function Oxygenases / Q000378:metabolism*
D006899:Mixed Function Oxygenases; C121463:gibberellin 2-dioxygenase