title: The histological features of anteromedial gonarthrosis--the comparison of two grading systems in a human phenotype of osteoarthritis.
abstract: Anteromedial gonarthrosis (AMG) displays a well recognised pattern of cartilage damage on the medial tibial plateau. Anteriorly there is a full thickness cartilage defect, with transition to a partial thickness defect, becoming full thickness cartilage in the posterior third of the tibial plateau. The retained posterior cartilage is macroscopically normal. This study characterises the histological changes of AMG and examines the usefulness of two histological assessment tools. Sixteen unicompartmental resection specimens of patients with primary AMG were assessed. Samples were stained with Haematoxylin and Eosin and Safranin-O stains and scored using the modified Mankin grade, and the OOCHAS assessment tool. Each specimen was assessed at five regions along the antero-posterior axis starting from the exposed bone to the region of macroscopically normal cartilage. From anterior to posterior the staining showed a consistent increase in structural integrity and cellularity of the cartilage, matched by a qualitative increase in GAG content. Mean modified Mankin and OOCHAS scores showed a progressive decrease in grade (p < 0.001). The OOCHAS grade had a good correlation with the modified Mankin grade (ρ = 0.886) and there was good intra- and inter-observer variability with both assessment tools. We conclude that there is progressive decrease in histological score from anterior to posterior in AMG and that the macroscopically normal cartilage seen posteriorly is histologically normal. Both the modified Mankin and OOOCHAS assessment tools are useful in histological grading but we found the OOCHAS easier and quicker to use. We propose that AMG represents a spatial model of progressive cartilage damage.
authors.affiliation: Biomedical Research Unit, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Nuffield Orthopaedic Centre, Headington, Oxford, OX3 7LD, United Kingdom.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Rout
authors.forename: Stephen
authors.initials: S
authors.lastname: McDonnell
authors.forename: Richard
authors.initials: R
authors.lastname: Benson
authors.forename: Nicholas
authors.initials: N
authors.lastname: Athanasou
authors.forename: Andrew
authors.initials: A
authors.lastname: Carr
authors.forename: Helen
authors.initials: H
authors.lastname: Doll
authors.forename: Harinderjit S
authors.initials: HS
authors.lastname: Gill
authors.forename: David W
authors.initials: DW
authors.lastname: Murray
authors.forename: Philippa A
authors.initials: PA
authors.lastname: Hulley
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Price
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007719:Knee Joint / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D020370:Osteoarthritis, Knee / Q000145:classification* / Q000473:pathology; D010641:Phenotype; D015203:Reproducibility of Results; D012720:Severity of Illness Index