Non-replicating adenovirus vectors expressing avian influenza virus hemagglutinin and nucleocapsid proteins induce chicken specific effector, memory and effector memory CD8(+) T lymphocytes.
Avian influenza virus (AIV) specific CD8(+) T lymphocyte responses stimulated by intramuscular administration of an adenovirus (Ad) vector expressing either HA or NP were evaluated in chickens following ex vivo stimulation by non-professional antigen presenting cells. The CD8(+) T lymphocyte responses were AIV specific, MHC-I restricted, and cross-reacted with heterologous H7N2 AIV strain. Specific effector responses, at 10 days post-inoculation (p.i.), were undetectable at 2 weeks p.i., and memory responses were detected from 3 to 8 weeks p.i. Effector memory responses, detected 1 week following a booster inoculation, were significantly greater than the primary responses and, within 7 days, declined to undetectable levels. Inoculation of an Ad-vector expressing human NP resulted in significantly greater MHC restricted, activation of CD8(+) T cell responses specific for AIV. Decreases in all responses with time were most dramatic with maximum activation of T cells as observed following effector and effector memory responses.
Singh
Shailbala
S
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Toro
Haroldo
H
Tang
De-Chu
DC
Briles
Worthie E
WE
Yates
Linda M
LM
Kopulos
Renee T
RT
Collisson
Ellen W
EW
D000256:Adenoviridae; D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006388:Hemagglutinins / Q000378:metabolism*; D009980:Influenza A virus / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D007371:Interferon-gamma / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D014765:Viral Vaccines / Q000276:immunology*; D014779:Virus Replication
D006388:Hemagglutinins; D019590:Nucleocapsid Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma