title: T-vector and in vivo recombination as tools to construct a large antibody library of breast cancer.
abstract: The emergence of phage antibody libraries is an important advance in the field of antibody engineering. It provides a useful methodology to produce human antibodies and has the potential to replace traditional hybridoma technology. In our research, we used T-vector and in vivo recombination to construct a large antibody library from breast cancer patients. The use of T-vector considerably increased the cloning efficiency, and the diversity of the library could be increased easily using in vivo recombination. Taken together, a combination of these two techniques might be valuable in constructing a large antibody library.
authors.affiliation: Department of Vascular and Endocrine Surgery, First Affiliated Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Lv
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Shi-Fang
authors.initials: SF
authors.lastname: Yuan
authors.forename: Nan-Lin
authors.initials: NL
authors.lastname: Li
authors.forename: Jiang-Hao
authors.initials: JH
authors.lastname: Chen
authors.forename: Ai-Zhi
authors.initials: AZ
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Ling
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D019151:Peptide Library; D011995:Recombination, Genetic
chemical_list: D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D019151:Peptide Library