Molecular epidemiology of porcine epidemic diarrhea virus in China.
Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field strains (excluding CH/GSJIII/07) contain a single 672- or 675-nucleotide (nt) ORF, which encodes a 223- or 224-aa-long peptide. However, the CV777 vaccine strain and CH/GSJIII/07 contain a 276-nt ORF because of a 49-nt deletion at nt 245-293. The Chinese PEDV field strains and PEDV reference strains are divided into three groups based on the phylogenetic relationship of their ORF3 genes. Chinese PEDV field strains (excluding CH/GSJIII/07) have a close phylogenetic relationship to Korean strains and are genetically different from the PEDV vaccine strains. However, CH/GSJIII/07 has a close phylogenetic relationship to two vaccine strains, suggesting that it might have evolved from a live vaccine strain. Chinese PEDV field strains (excluding CH/GSJIII/07) can be differentiated from PEDV vaccine strains by a nested RT-PCR method.
Chen
Jianfei
J
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Wang
Chengbao
C
Shi
Hongyan
H
Qiu
Huaji
H
Liu
Shengwang
S
Chen
Xiaojin
X
Zhang
Zhibang
Z
Feng
Li
L
D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D014764:Viral Proteins