One-step, aid-mediated method for modification of glass surfaces with N-hydroxysuccinimide esters and its application to the construction of microarrays for studies of biomolecular interactions.
Microarray technologies have received considerable attention owing to the fact that they serve as powerful tools for the high-throughput analysis of biomolecular interactions and the identification of bioactive substances that bind to biomolecules. Most of the current methods used to construct microarrays rely on the immobilization of substances on properly derivatized surfaces. Among various functional groups used for this purpose, the N-hydroxysuccinimide (NHS) ester group has been largely employed because it can be readily reacted with amine or hydrazide functionalities in substances of interest. However, the NHS ester group is usually introduced onto the surface of a glass slide by employing inconvenient and time-consuming multistep processes. In recent studies, we have developed an efficient, single step method for derivatization of glass surfaces with NHS ester groups that takes advantage of an acid-mediated reaction of NHS ester functionalized dimethallylsilanes with silanols on the glass surface. Conditions for the surface modification procedure that utilize TfOH rather than Sc(OTf)(3) were found to be superior. Protein and RNA-binding experiments show that glass surfaces modified by employing this method are suitable for efficient immobilization of various substances that are appended by amine, hydrazide, and alcohol functionalities. The microarrays, generated in this way, are applicable to procedures for rapid analysis of protein-protein, protein-glycan, protein-small molecule, and peptide-RNA interactions, as well as for profiling enzyme activities. The newly developed acid-mediated, glass surface modification method should be generally applicable to the preparation of various functional group-modified surfaces.
Park
Sungjin
S
Center for Biofunctional Molecules, Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Pai
Jaeyoung
J
Han
Eun-Hee
EH
Jun
Chul-Ho
CH
Shin
Injae
I
D000143:Acids / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D004952:Esters / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry*; D013499:Surface Properties
D000143:Acids; D000588:Amines; D004952:Esters; D006834:Hydrazines; D010455:Peptides; D011134:Polysaccharides; D011506:Proteins; D012821:Silanes; D013388:Succinimides; C082343:silanol; D012313:RNA; C001426:N-hydroxysuccinimide