Techniques of dental DNA extraction: Some operative experiences.
This study presents and discusses the possibility of multiple dental DNA extraction at different times using an endodontic technique. We chose a mixed sample of twenty teeth (decayed, filled, different kinds of teeth, etc.) and performed two different accesses to the pulp cavity to collect samples of dental tissue useful for DNA extraction. The Identifiler(®) and Minifiler(®) kits were used respectively for the first and second genetic analysis. The research's most relevant findings are the possibility of successfully repeating dental DNA extractions, and that a dental element can be considered as a DNA source that can be reused even after prolonged time elapses. The study's results document a simple and efficient methodology for dental identification based on the choice of dental elements, endodontic techniques and the use of the Minifiler(®) kit for genetic analysis.
Pinchi
Vilma
V
Department of Anatomy, Histology and Legal Medicine, Legal-Medicine Section, University of Florence, Viale Morgagni 85, 50134 Florence, Italy. pinchi@unifi.it
Torricelli
Francesca
F
Nutini
A Lucia
AL
Conti
Marco
M
Iozzi
Sara
S
Norelli
Gian-Aristide
GA
D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D003782:Dental Pulp / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005553:Forensic Dentistry / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D014081:Tooth Extraction
D004247:DNA