title: Probing oxidative stress in single erythrocytes with Raman Tweezers.
abstract: Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to SS and C-S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.
authors.affiliation: Centre for Atomic and Molecular Physics, Manipal University, Manipal 576 104, India.
authors.forename: E
authors.initials: E
authors.lastname: Zachariah
authors.forename: A
authors.initials: A
authors.lastname: Bankapur
authors.forename: C
authors.initials: C
authors.lastname: Santhosh
authors.forename: M
authors.initials: M
authors.lastname: Valiathan
authors.forename: D
authors.initials: D
authors.lastname: Mathur
mesh_terms: D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000494:pharmacology; D052898:Optical Tweezers; D018384:Oxidative Stress; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman
chemical_list: D017665:Hydroxyl Radical; D005978:Glutathione