title: Genotoxicity testing of three monohaloacetic acids in TK6 cells using the cytokinesis-block micronucleus assay.
abstract: Chemical disinfection of water generates harmful chemical compounds, known as disinfection by-products (DBPs). One class of DBPs is constituted by haloacetic acids (HAAs), the second major group in prevalence (after trihalomethanes) detected in finished drinking water. In this article, we report the results obtained in the evaluation of the chromosome damage induced by three monohaloacetic acids, namely iodoacetic acid (IAA), bromoacetic acid (BAA) and chloroacetic acid (CAA). To evaluate the induction of chromosome damage, we used the cytokinesis-block micronucleus test that measures the ability of genotoxic agents to induce both clastogenic and/or aneugenic effects. No previous data exist on the effects of these compounds on human chromosomes. We tested five doses of each HAA, in addition to the negative and positive controls. The highest dose tested for each HAA was that immediately lower than the dose producing total cytotoxicity. Our results show that none of the three HAAs tested was able to increase significantly the frequency of micronucleus in binucleated TK6 cells, the rank order in decreasing cytotoxicity was IAA > BAA >> CAA.
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain.
authors.forename: Danae
authors.initials: D
authors.lastname: Liviac
authors.forename: Amadeu
authors.initials: A
authors.lastname: Creus
authors.forename: Ricard
authors.initials: R
authors.lastname: Marcos
mesh_terms: D000085:Acetates / Q000633:toxicity; D019342:Acetic Acid / Q000633:toxicity*; D002478:Cells, Cultured; D048749:Cytokinesis / Q000187:drug effects*; D006801:Humans; D019807:Iodoacetic Acid / Q000633:toxicity; D015162:Micronucleus Tests / Q000379:methods*; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*
chemical_list: D000085:Acetates; D009153:Mutagens; C006972:chloroacetic acid; C016274:bromoacetate; D019342:Acetic Acid; D019807:Iodoacetic Acid