Improved biomolecule microarrays by printing on nanoporous aluminum oxide using a continuous-flow microspotter.
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods. The spots are observed with a fluorescence scanner. By using the CFM to print spots on nanoporous substrates, an additional order of magnitude increase in signal is observed, which leads to high signal-to-background ratios, highly saturated spots, and a measurable signal at printing concentrations as low as 1.6 ng mL(-1). This technique produces highly concentrated biomolecular spots from dilute samples and significantly increases the sensitivity of sensing platforms.
Kim
Jungkyu
J
Department of Bioengineering University of Utah Salt Lake City, UT 84112, USA. jungkyu.kim@utah.edu
Miles
Adam
A
Gale
Bruce K
BK
D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D005453:Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D011437:Propylamines; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D012212:Rheology; D012710:Serum Albumin, Bovine / Q000032:analysis; D012821:Silanes / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000032:analysis; D013499:Surface Properties
D011437:Propylamines; D012821:Silanes; D013205:Staphylococcal Protein A; D012710:Serum Albumin, Bovine; C477625:amino-propyl-triethoxysilane; D000537:Aluminum Oxide