Research of glycosylated granulocyte colony-stimulating factor on mobilizing cell regeneration of bone marrow stem cells and repairing injured myocardium.
OBJECTIVE
To compare the differences between glycosylated and non-glycosylated granulocyte colony-stimulating factor (G-CSF) on mobilizing cell regeneration of bone marrow stem cells and repairing injured myocardium.


METHODS
In the acute myocardial ischemia model, 40 Sprague-Dawley rats (8 weeks old, weight 200-250 g) were successfully established with isoproteronol (ISO) and randomly and evenly divided into 4 groups: Group G (injected with glycosylated G-CSF), Group N (non-glycosylated G-CSF), Group C ([control] normal saline for 7 days), and Group M (model). At day 5, all rats were labeled with bromodeoxyuridine (BrdU). At day 28, the proportion of CD34+ cells, myocardial BrdU immunohistochemical stain, myocardial capillary density, myocardial fibrosis and cardiac function was detected among the 3 groups. The study was carried out at the Central Laboratory, Tongji Hospital of Tongji University, Shanghai, China between 1 July 2009 and 31 July 2009.


RESULTS
In Group G, the proportion of CD34+ cells and cardiac function was significantly higher than groups N and C, and BrdU positive cells in myocardium were higher than those other groups. Diaminobenzidine stained positive cells in striated muscle tissue possessed more cardiocyte-like structure, higher myocardial capillary density, and less myocardial fibrosis in group G compared with other 2 groups.


CONCLUSIONS
Glycosytated G-CSF might possess stronger capability to mobilize cell regeneration of bone marrow stem cells and repair injured myocardium.
Ma
Yuan
Y
Department of Cardiology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang Province, China.
Luo
Ming
M
D000818:Animals; D006031:Glycosylation; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D009203:Myocardial Infarction / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016179:Granulocyte Colony-Stimulating Factor