Effect of redox conditions on bacterial and fungal biomass and carbon dioxide production in Louisiana coastal swamp forest sediment.
Fungal and bacterial carbon dioxide (CO2) production/emission was determined under a range of redox conditions in sediment from a Louisiana swamp forest used for wastewater treatment. Sediment was incubated in microcosms at 6 Eh levels (-200, -100, 0, +100, +250 and +400 mV) covering the anaerobic range found in wetland soil and sediment. Carbon dioxide production was determined by the substrate-induced respiration (SIR) inhibition method. Cycloheximide (C15H23NO4) was used as the fungal inhibitor and streptomycin (C21H39N7O12) as the bacterial inhibitor. Under moderately reducing conditions (Eh > +250 mV), fungi contributed more than bacteria to the CO2 production. Under highly reducing conditions (Eh < or = 0 mV), bacteria contributed more than fungi to the total CO2 production. The fungi/bacteria (F/B) ratios varied between 0.71-1.16 for microbial biomass C, and 0.54-0.94 for microbial biomass N. Under moderately reducing conditions (Eh > or = +100 mV), the F/B ratios for microbial biomass C and N were higher than that for highly reducing conditions (Eh < or = 0 mV). In moderately reducing conditions (Eh > or = +100 mV), the C/N microbial biomass ratio for fungi (C/N: 13.54-14.26) was slightly higher than for bacteria (C/N: 9.61-12.07). Under highly reducing redox conditions (Eh < or = 0 mV), the C/N microbial biomass ratio for fungi (C/N: 10.79-12.41) was higher than for bacteria (C/N: 8.21-9.14). For bacteria and fungi, the C/N microbial biomass ratios under moderately reducing conditions were higher than that in highly reducing conditions. Fungal CO2 production from swamp forest could be of greater ecological significance under moderately reducing sediment conditions contributing to the greenhouse effect (GHE) and the global warming potential (GWP). However, increases in coastal submergence associated with global sea level rise and resultant decrease in sediment redox potential from increased flooding would likely shift CO2 production to bacteria rather than fungi.
Seo
Dong Cheol
DC
Department of Oceanography and Coastal Sciences, School of the Coast and Environment, Louisiana State University, Baton Rouge, LA 70803, USA.
DeLaune
Ronald D
RD
D000693:Anaerobiosis; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis* / Q000378:metabolism; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D008145:Louisiana; D010084:Oxidation-Reduction; D014197:Trees; D053833:Wetlands
D002245:Carbon Dioxide; D002244:Carbon