Rubellimicrobium roseum sp. nov., a Gram-negative bacterium isolated from the forest soil sample.
A novel pink-coloured, non-spore-forming, non-motile, Gram-negative bacterium, designated YIM 48858(T), is described by using a polyphasic approach. The strain can grow at pH 6.5-9 (optimum at pH 7) and 25-30 degrees C (optimum at 28 degrees C). NaCl is not required for its growth. Positive for oxidase and catalase. Urease activity, nitrate reduction, starch and Tween 80 tests are negative reaction. 16S rRNA gene sequence similarity studies showed that strain YIM 48858(T) is a member of the genus Rubellimicrobium, with similarities of 96.3, 95.7 and 95.5% to Rubellimicrobium mesophilum MSL-20(T), Rubellimicrobium aerolatum 5715S-9(T) and Rubellimicrobium thermophilum DSM 16684(T), respectively. Q-10 was the predominant respiratory ubiquinone as in the other members of the genus Rubellimicrobium. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphoglycolipid, glycolipid and the major fatty acids were C18:1 omega7c, C16:0 and C10:0 3-OH, which are very different from the valid published species. The DNA G + C content was 67.7 mol%. Both phylogenetic and chemotaxonomic evidence supports that YIM 48858(T) is a novel species of the genus Rubellimicrobium, for which the name Rubellimicrobium roseum sp. nov. is proposed. The type strain is YIM 48858(T) (=CCTCC AA 208029(T) =KCTC 23202(T)).
Cao
Yan-Ru
YR
College of Resources and Environment, Northwest A & F University, Yangling 712100, Shaanxi, People's Republic of China.
Jiang
Yi
Y
Wang
Qian
Q
Tang
Shu-Kun
SK
He
Wen-Xiang
WX
Xue
Quan-Hong
QH
Xu
Li-Hua
LH
Jiang
Cheng-Lin
CL
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012987:Soil; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil