title: In vitro osteogenic differentiation of HOS cells on two types of collagen gels.
abstract: HOS cell is a model strain of human osteoblasts derived from human osteosarcoma. We cultured the HOS cells on both the conventional collagen gel (neutral gel), and the gamma-crosslinked collagen gel without collagen fibrils (acidic gel). The shape of HOS cells on the neutral gel was similar to that on the culture dish. However, HOS cells on acidic gel had an elongated shape and attached each other to form a mesh-like pattern. The cells attached to the surface of both gels but scarcely penetrated their depths. We measured the biochemical markers for osteogenic differentiation in the HOS cells cultured on both the neutral gel and the acidic gel. The expressions of alkaline phosphatase and osteocalcin were detected in the HOS cells on both types of collagen gel. Deposition of the calcium also occurred on both gels although it was higher in the neutral gel than the acidic one. These results indicate the importance of collagen for the differentiation of HOS cells, but it is not dependent on the molecular structure (fibril formation) of collagen.
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Takitoh
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kato
authors.forename: Asako
authors.initials: A
authors.lastname: Nakasu
authors.forename: Mika
authors.initials: M
authors.lastname: Tadokoro
authors.forename: Masahiko
authors.initials: M
authors.lastname: Bessho
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.forename: Hideki
authors.initials: H
authors.lastname: Mori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
mesh_terms: D001483:Base Sequence; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D017931:DNA Primers; D006801:Humans; D010012:Osteogenesis; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D003094:Collagen