[Effect of 2,5-hexanedione on myelin protein zero in sciatic nerve and its antibody in serum of rats at different time points].
OBJECTIVE
To explore the effects of 2,5-hexandione, the metabolite of n-hexane, on the expression of myelin protein zero (P0) in sciatic nerve and on the positive rate of P0 antibody in serum of rats at different time points.


METHODS
Seventy five Wistar rats were divided into five groups and were administrated with 400 mg/kg 2,5-hexanedione per day for 0, 1, 2, 3 and 4 weeks respectively. The P0 expression at different time points was determined with immunohischemistry and the P0 antibody in serum were detected with enzyme-linked immunosorbent assay.


RESULTS
With the administration of 2,5-hexandione, the rats gradually showed the signs of peripheral neuropathy. P0 distribution in transverse section of sciatic nerve was different, and the intensity in myelin sheath was higher than that in axon. The expression of P0 in sciatic nerve of rats with 2,5-hexanedione administration for 0 week seemed higher than those of the other time points, and the expression of the P0 showed a decreasing tendency with the time of 2,5-hexanedione administration. The positive rate of P0 antibody in serum of rats administrated with 400 mg/kg 2,5-hexanedione for 0, 1, 2, 3 and 4 weeks were 33.3%, 26.7%, 46.7%, 46.7% and 84.6% respectively. The positive rate of Po antibodies in serum of rats showed an increasing tendency with the time of 2,5-hexanedione administration (chi2 = 11.007, P < 0.05).


CONCLUSIONS
The P0 in sciatic nerve of rats could be destroyed by 2,5-hexanedione and P0 expression level decreased with the time of 2,5-hexanedione administration. The positive rate of P0 antibody in serum increased with the time of 2,5-hexanedione administration in rats.
Liu
Qingjun
Q
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhao
Lei
L
Duan
Huawei
H
Dai
Yufei
Y
Niu
Yong
Y
Chen
Hong
H
Liu
Qing
Q
Bin
Ping
P
Zheng
Yuxin
Y
D000818:Animals; D000906:Antibodies / Q000097:blood*; D006588:Hexanones / Q000633:toxicity*; D008297:Male; D018993:Myelin P0 Protein / Q000276:immunology* / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D000906:Antibodies; D006588:Hexanones; D018993:Myelin P0 Protein; C011269:2,5-hexanedione