[Gene expression in patients with myelodysplastic syndrome].
This study was aimed to investigate the expression of c-FLIPL, c-FLIPS and DLK1 mRNA in the patients with myelodysplastic syndrome (MDS) and its clinical significance. The mRNA expression of c-FLIPL, c-FLIPS and DLK1 in bone marrow mononuclear cells (BMMNC) of 16 patients with MDS and 3 controls were detected by RT-PCR. The results indicated that the expression of DLK1 mRNA was up-regulated in MDS, including RA and RAEB, as compared with controls (p < 0.05). There was no significant difference in expression of DLK1 between RA and RAEB patients (p > 0.05); the expression of c-FLIPL mRNA both in RA and RAEB patients was higher than that in controls (p < 0.05). There was no significant difference in expression of c-FLIPL between RA and RAEB patients (p > 0.05); the expression of c-FLIPS mRNA was not significantly different between MDS patients and controls (p > 0.05), but its expression in RAEB patients was significantly higher as compared with RA patients and controls (p < 0.05). It is concluded that the mRNA expressions of DLK1, c-FLIPL and c-FLIPS in MDS patients are abnormal, some of which may be useful as an important indicator for the evaluation of development in MDS.
Chen
Bao-An
BA
Department of Hematology, Zhongda Hospital, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China. cba8888@hotmail.com
Zhang
Bo
B
Gao
Chong
C
Gao
Feng
F
Xia
Guo-Hua
GH
Shao
Ze-Ye
ZY
Ding
Jia-Hua
JH
Zhao
Gang
G
Chen
Jian
J
Wang
Jun
J
Song
Hui-Hui
HH
Bao
Wen
W
Zhong
Yue-Jiao
YJ
Pei
Xiao-Ping
XP
Wang
Fei
F
Gu
Zhong-Ze
ZZ
D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C506386:CFLAR protein, human; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger