title: A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection.
abstract: We herein report the design of a dumbbell-shaped DNA probe that integrates target-binding, amplification and signaling within one multifunctional design. The dumbbell probe can initiate rolling circle amplification (D-RCA) in the presence of specific microRNA (miRNA) targets. This D-RCA-based miRNA strategy allows quantification of miRNA with very low quantity of RNA samples. The femtomolar sensitivity of D-RCA compares favorably with other existing technologies. More significantly, the dynamic range of D-RCA is extremely large, covering eight orders of magnitude. We also demonstrate miRNA quantification with this highly sensitive and inexpensive D-RCA strategy in clinical samples.
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Jimin
authors.initials: J
authors.lastname: Gao
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D052160:Benzothiazoles; D014408:Biomarkers, Tumor / Q000032:analysis; D015342:DNA Probes / Q000737:chemistry*; D003959:Diamines; D005456:Fluorescent Dyes; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009930:Organic Chemicals; D011804:Quinolines; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D014408:Biomarkers, Tumor; D015342:DNA Probes; D003959:Diamines; D005456:Fluorescent Dyes; D035683:MicroRNAs; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I