Rat monoclonal antibody specific for MyoD.
Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.
Harada
Akihito
A
Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ohkawa
Yasuyuki
Y
Ao
Shinpei
S
Odawara
Jun
J
Okada
Seiji
S
Azuma
Masayuki
M
Nishiyama
Yuko
Y
Nakamura
Mako
M
Tachibana
Taro
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D015153:Blotting, Western; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D022041:Euchromatin / Q000276:immunology; D005260:Female; D006825:Hybridomas; D007082:Ileum / Q000276:immunology; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000254:growth & development / Q000276:immunology*; D017570:MyoD Protein / Q000276:immunology*; D032446:Myoblasts / Q000276:immunology* / Q000648:ultrastructure; D051381:Rats; D011921:Rats, Inbred WKY
D000911:Antibodies, Monoclonal; D022041:Euchromatin; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein