Extracellular domain dependence of PTPalpha transforming activity.
Two isoforms of the transmembrane protein tyrosine phosphatase PTPalpha, which differ by nine amino acids in their extracellular regions, are expressed in a tissue-specific manner. Over-expression of the shorter isoform transforms rodent cells, and it has previously been reasonable to assume that this was a direct consequence of its dephosphorylation and activation of Src. Transformation by the longer wild-type isoform has not previously been studied. We tested the activities of both isoforms in NIH3T3 cells and found that, while both dephosphorylated and activated Src similarly, only the shorter isoform induced focus formation or anchorage-independent growth. Differences in phosphorylation of PTPalpha at its known regulatory sites, Grb2 binding to PTPalpha, phosphorylation level of focal adhesion kinase by PTPalpha, or overall localization were excluded as possible explanations for the differences in transforming activities. The results suggest that transformation by PTPalpha involves at least one function other than, or in addition to, its activation of Src and that this depends on PTPalpha's extracellular domain. Previous studies have suggested that PTPalpha might be a useful target in breast and colon cancer therapy, and the results presented here suggest that it may be advantageous to develop isoform-specific therapeutic reagents.
Tremper-Wells
Barbara
B
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Resnick
Ross J
RJ
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Zheng
Xinmin
X
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Holsinger
Leslie J
LJ
Virobay, Menlo Park, CA 94025, USA.
Shalloway
David
D
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4 / Q000737:chemistry* / Q000378:metabolism*
D007527:Isoenzymes; C516843:Ptpra protein, mouse; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4