Human leukocyte antigen typing in tuberculous rheumatism: Poncet's disease.
METHODS
Tuberculous rheumatism (Poncet's disease) is a reactive polyarthropathy associated with extra-pulmonary and pulmonary tuberculosis (TB) without evidence of mycobacterial infection of the involved joints. As all patients with TB do not present with this peculiar clinical feature, a genetic susceptibility is suspected.


OBJECTIVE
To determine the major histocompatibility complex (MHC) class I and II alleles in Mexican mestizo patients with Poncet's disease.


METHODS
In this case-control study of 16 Mexican mestizo patients diagnosed with Poncet's disease and 99 ethnically matched healthy controls, high resolution human leukocyte antigen (HLA) typing was performed for HLA-A, B, DR and DQ by polymerase chain reaction. HLA-DRB1 and HLA-DQB1 subtypes were performed by sequence-specific oligonucleotide probe hybridization.


RESULTS
A significantly increased frequency of HLA-B27 (corrected P = 0.01) and DQB1*0301 (corrected P = 0.0009) alleles and decreased frequency of HLA-DQB1*0302 (corrected P = 0.00001) were identified in patients compared to healthy controls.


CONCLUSIONS
These data suggest that genes located within the MHC may play a role in the susceptibility to Poncet's disease in patients diagnosed with TB.
Lugo-Zamudio
G E
GE
Department of Rheumatology, Hospital Juárez de México, México DF, México. gelz1@prodigy.net.mx
Yamamoto-Furusho
J K
JK
Delgado-Ochoa
D
D
Nuñez-Farfán
R M
RM
Vargas-Alarcón
G
G
Barbosa-Cobos
R E
RE
Granados
J
J
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005805:Genes, MHC Class I / Q000235:genetics; D005802:Genes, MHC Class II / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006680:HLA Antigens / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D014394:Tuberculosis, Osteoarticular / Q000235:genetics*; D055815:Young Adult
D006680:HLA Antigens; D015796:HLA-B27 Antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015345:Oligonucleotide Probes