Polar-copolymerized approach based on horizontal polymerization on silica surface for preparation of polar-modified stationary phases.
A new approach for preparation of polar-modified reversed-phase liquid chromatography stationary phases was developed by using horizontal polymerization technique on silica surface, which was defined as "polar-copolymerized" approach. Based on this new approach, a representative polar-copolymerized stationary phase composed of mixed n-octadecyl and chloropropyl (C18-C3Cl) was synthesized. The resulting stationary phase named C18HCE was characterized with elemental analysis and solid phase 13C and 29Si NMR, which proved the chemistry of polar-copolymerized stationary phases. Chromatographic evaluation and application of the C18HCE were also investigated. The results of preliminary chromatographic evaluation demonstrated that the C18HCE stationary phase exhibited 100% aqueous mobile phase compatibility, low silanol activity. In addition, the application results demonstrated that the C18HCE had superior separation performance in alkaloids separation at acidic conditions compared to some commercial stationary phases.
Guo
Zhimou
Z
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Wang
Chaoran
C
Liang
Tu
T
Liang
Xinmiao
X
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D031311:Corydalis / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D000470:Alkaloids; D011108:Polymers; D012822:Silicon Dioxide