Sequential changes in chromatin structure during transcriptional activation in the beta globin LCR and its target gene.
Chromatin structure is modulated during transcriptional activation. The changes include the association of transcriptional activators, formation of hypersensitive sites and covalent modifications of histones. To understand the order of the various changes accompanying transcriptional activation, we analyzed the mouse beta globin gene, which is transcriptionally inducible in erythroid MEL cells over a time course of HMBA treatment. Transcription of the globin genes requires the locus control region (LCR) consisting of several hypersensitive sites (HSs). Erythroid specific transcriptional activators such as NF-E2, GATA-1, TAL1 and EKLF were associated with the LCR in the uninduced state before transcriptional activation. The HSs of the LCR were formed in this state as revealed by high sensitivity to DNase I and MNase attack. However the binding of transcriptional activators and the depletion of histones were observed in the promoter of the beta globin gene only after transcriptional activation. In addition, various covalent histone modifications were sequentially detected in lysine residues of histone H3 during the activation. Acetylation of K9, K36 and K27 was notable in both LCR HSs and gene after induction but before transcriptional initiation. Inactive histone marks such as K9me2, K36me2 and K27me2 were removed coincident with transcriptional initiation in the gene region. Taken together, these results indicate that LCR has a substantially active structure in the uninduced state while transcriptional activation serially adds active marks, including histone modifications, and removes inactive marks in the target gene of the LCR.
Kim
Kihoon
K
Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, Republic of Korea.
Kim
AeRi
A
D000081:Acetamides / Q000494:pharmacology; D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D006657:Histones / Q000378:metabolism; D019901:Locus Control Region / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D055544:beta-Globins / Q000235:genetics*
D000081:Acetamides; D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D055544:beta-Globins; C014026:hexamethylene bisacetamide