title: Proteomic analysis of interstitial fluid in bone marrow identified that peroxiredoxin 2 regulates H(2)O(2) level of bone marrow during aging.
abstract: Hematopoiesis in bone marrow declines during aging owing to alteration of the hematopoietic niche. However, due to difficult accessibility and other complexities, senescence-related alteration of the hematopoietic niche is largely unknown. The interstitial fluid of bone marrow (IFBM), a pivotal component of the hematopoietic niche, includes soluble secretory factors that are present between bone marrow cells. To characterize the proteomic profile changes of IFBM during aging, we analyzed the IFBMs of young, adult, and senescent rats using 2-DE combined with ESI/MALDI-Q-TOF MS. Finally, 31 differentially expressed proteins involved in multiple biological functions were identified. Peroxiredoxin 2 (Prx2), down-regulated during aging, was further analyzed and demonstrated that it is produced by bone marrow stromal cells. Interestingly, higher levels of hydrogen peroxide (H(2)O(2)) were detected in the bone marrow with lower Prx2 expression. Moreover, exogenous Prx2 reduced the intracellular H(2)O(2) level in bone marrow stromal cells in vitro. Therefore, Prx2 is implied in the regulation of H(2)O(2) production in the bone marrow during aging. Our data characterized the dynamic protein profiles of the bone marrow microenvironment during aging and we provided clues to elucidate the mechanism of creating a low ROS level in the hematopoietic niche.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital and College of Life Sciences, Sichuan University, Chengdu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lantu
authors.initials: L
authors.lastname: Gou
authors.forename: Gang
authors.initials: G
authors.lastname: Xie
authors.forename: Aiping
authors.initials: A
authors.lastname: Tong
authors.forename: Fei
authors.initials: F
authors.lastname: He
authors.forename: Zejun
authors.initials: Z
authors.lastname: Lu
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Yao
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Minghai
authors.initials: M
authors.lastname: Tang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Jinliang
authors.initials: J
authors.lastname: Yang
authors.forename: Huozhen
authors.initials: H
authors.lastname: Hu
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D045604:Extracellular Fluid / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D013058:Mass Spectrometry; D054464:Peroxiredoxins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D006861:Hydrogen Peroxide; D054464:Peroxiredoxins