title: The effect of salvianolic acid B combined with laminar shear stress on TNF-alpha-stimulated adhesion molecule expression in human aortic endothelial cells.
abstract: The study was conducted to investigate the effect of Salvianolic acid B (Sal B) on TNF-alpha-stimulated adhesion molecule expression i.e. vascular adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and E-selectin in human aortic endothelial cells (HAECs) under laminar shear stress (LSS) condition. Exposure of HAECs to LSS (12 dynes/cm(2) for 6 h decreased the TNF-alpha-induced protein expression of adhesion molecules i.e. VCAM-1, ICAM-1 and E-selectin. Pre-treatment of HAECs with Sal B (10 microg/ml) then exposed to LSS (12 dynes/cm(2)) for 6 h significantly inhibited VCAM-1, ICAM-1 and E-selectin expression stimulated by TNF-alpha. Moreover, combined Sal B and LSS treatment inhibited the adhesiveness of monocytic U937 cells to TNF-alpha-stimulated HAECs. We further examined the molecular mechanisms and found that the combination of Sal B and LSS treatment dramatically inhibited TNF-alpha-induced NF-kappaB activation evidenced by IkappaBalpha degradation and p65 nuclear translocation in HAECs. This study provides the first biomechanopharmacological evidence that Sal B has a combination effect with LSS to reduce the expression of three adhesion molecules, leading to reduced monocyte adhesion to HAECs, at least in part, by inhibiting the NF-kappaB signaling pathway. Data from this study thus support the potential clinical application of Sal B in vascular inflammatory diseases.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors.forename: Li Xia
authors.initials: LX
authors.lastname: Xie
authors.forename: Siva Sundara Kumar
authors.initials: SS
authors.lastname: Durairajan
authors.forename: Jia Hong
authors.initials: JH
authors.lastname: Lu
authors.forename: Chen Li
authors.initials: CL
authors.lastname: Liu
authors.forename: Wan Fung
authors.initials: WF
authors.lastname: Kum
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Irene
authors.initials: I
authors.lastname: Koo
authors.forename: Wei Kang
authors.initials: WK
authors.lastname: Wu
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Fulong
authors.initials: F
authors.lastname: Lao
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001572:Benzofurans; D002448:Cell Adhesion / Q000187:drug effects; D019040:E-Selectin / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013314:Stress, Mechanical; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D020298:U937 Cells / Q000187:drug effects; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D053630:Depsides
chemical_list: D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C076944:salvianolic acid B; D053630:Depsides