[Genetic polymorphism in XPD related to risks of chronic benzene poisoning].
OBJECTIVE
To explore the relation between genetic polymorphisms in XPD and risks of chronic benzene poisoning (CBP).


METHODS
A case-control study was conducted. 152 CBP patients and 152 NCBP workers occupationally exposed to benzene were investigated. Polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms (SNPs) at c. 199, c. 201, c. 312 and c. 751 of XPD gene.


RESULTS
No variant alleles was detected at c. 199 and c. 201 of XPD gene. In comparition with the individual genotypes of XPDc. 312Asp/Asp, the risk of CBP suffered from the individual genotype of XPDc. 312Asp/Asn + Asn/Asn decreased a 0.59 fold (ORadj = 0.59, 95% CI = 0.35-0.99, chi2 = 3.99, P < 0.05), when sex, workage and intensity of benzene exposure were adjusted. And in low intensity of benzene exposure group, the risk of CBP suffered from the individual genotypes of XPDc. 312Asp/Asn + Asn/Asn more decreased (ORadj = 0.13, 95% CI = 0.04-0.51, chi2 = 8.93, P < 0.01).


CONCLUSIONS
Polymorphism of XPD Asp312Asn could contribute to altered risk of CBP.
Li
Yan
Y
Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China. liyan7635@yahoo.cn
Zhang
Zhongbin
Z
Sun
Pin
P
Wan
Junxiang
J
Jin
Xipeng
X
Xia
Zhaolin
Z
D000328:Adult; D001554:Benzene / Q000032:analysis / Q000506:poisoning*; D016022:Case-Control Studies; D002681:China; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011041:Poisoning / Q000235:genetics; D011110:Polymorphism, Genetic; D012307:Risk Factors; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*; D055815:Young Adult
D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D001554:Benzene