Comparison of the activities of the lantibiotics nisin and lacticin 3147 against clinically significant mycobacteria.
The aim of this study was to use the microtitre alamarBlue assay to investigate and compare the antimycobacterial potential of the lantibiotics nisin and lacticin 3147 against a representative cohort of clinically significant mycobacteria, i.e. Mycobacterium tuberculosis H37Ra, Mycobacterium avium subsp. paratuberculosis (MAP) ATCC 19698 and Mycobacterium kansasii CIT11/06. Lacticin 3147 displayed potent activity against all strains of mycobacteria, with MIC(90) values (lowest concentration of lantibiotic that prevented growth of >90% of the bacterial population) of 60 mg/L and 15 mg/L for M. kansasii and MAP, respectively. Lacticin 3147 was particularly effective against M. tuberculosis H37Ra, with a MIC(90) value of 7.5mg/L. Nisin, although inhibitory, was generally less potent against all strains of mycobacteria, with MIC(90) values of 60 mg/L for M. kansasii and >60 mg/L for MAP and M. tuberculosis H37Ra. Thus, lacticin 3147 is a potent antimycobacterial peptide that shows superior activity compared with nisin at physiological pH.
Carroll
James
J
Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
Draper
Lorraine A
LA
O'Connor
Paula M
PM
Coffey
Aidan
A
Hill
Colin
C
Ross
R Paul
RP
Cotter
Paul D
PD
O'Mahony
Jim
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D016927:Mycobacterium avium subsp. paratuberculosis / Q000187:drug effects*; D019909:Mycobacterium kansasii / Q000187:drug effects*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009561:Nisin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C082508:lacticin 481; D009561:Nisin