title: The transcriptional activity of Neurog3 affects migration and differentiation of ectopic endocrine cells in chicken endoderm.
abstract: Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells.
authors.affiliation: Department of Developmental Biology, Hagedorn Research Institute, Niels Steensens Vej 6, Gentofte, Denmark.
authors.forename: Louise C
authors.initials: LC
authors.lastname: Rosenberg
authors.forename: Merete L
authors.initials: ML
authors.lastname: Lafon
authors.forename: Jesper Karup
authors.initials: JK
authors.lastname: Pedersen
authors.forename: Hani
authors.initials: H
authors.lastname: Yassin
authors.forename: Jan Nygaard
authors.initials: JN
authors.lastname: Jensen
authors.forename: Palle
authors.initials: P
authors.lastname: Serup
authors.forename: Jacob
authors.initials: J
authors.lastname: Hecksher-Sørensen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002645:Chickens; D018274:Electroporation; D055098:Endocrine Cells / Q000166:cytology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins