title: Genomic analysis of the 55 kDa subunit of DNA polymerase epsilon in human intracranial neoplasms.
abstract: BACKGROUND
  Defects of some DNA polymerases have shown cancer associations, but there are only limited data on DNA polymerase (Pol) epsilon.
  
  
  METHODS
  We examined 26 human brain neoplasm DNA samples and 8 control blood samples (from Poland) for possible mutations in the entire coding region of the 55 kDa small subunit of human DNA Pol epsilon gene using polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, and sequence analysis of DNA.
  
  
  RESULTS
  One single base intronic transition in intron 14 was found. The AATT deletion previously found in some breast and colorectal tumors was not found in samples from brain neoplasms or controls, but it was found in 1/100 normal blood samples from South-West Finland.
  
  
  CONCLUSIONS
  We found no evidence that potential mutations in the 55 kDa subunit of DNA Pol epsilon are a contributing factor in the development of the tested cases of human intracranial tumors.
authors.affiliation: Department of Pathology, University of Turku, FIN-20520 Turku, Finland.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Tuomo
authors.initials: T
authors.lastname: Lehtonen
authors.forename: Jaana
authors.initials: J
authors.lastname: Lehtonen
authors.forename: Susana
authors.initials: S
authors.lastname: Laakso
authors.forename: Janusz
authors.initials: J
authors.lastname: Szymas
authors.forename: Helmut
authors.initials: H
authors.lastname: Pospiech
authors.forename: Kirsi
authors.initials: K
authors.lastname: Huoponen
authors.forename: Juhani
authors.initials: J
authors.lastname: Syväoja
authors.forename: Yrjö
authors.initials: Y
authors.lastname: Collan
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004257:DNA Polymerase II / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D004273:DNA, Neoplasm; D004257:DNA Polymerase II