title: [Characterization of the variable region within class 1 integrons in Klebsiella pneumoniae nosocomials strains].
abstract: BACKGROUND
  Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria.
  
  
  OBJECTIVE
  To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae.
  
  
  METHODS
  Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation.
  
  
  RESULTS
  Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found.
  
  
  CONCLUSIONS
  The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.
authors.affiliation: Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, Estado Sucre, Venezuela. miltzaguz@cantv.net
authors.forename: Militza
authors.initials: M
authors.lastname: Guzmán
authors.forename: Guillermina
authors.initials: G
authors.lastname: Alonso
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial