Development of serratiopeptidase and metronidazole based alginate microspheres for wound healing.
The objective of this study was to establish an effective therapy system for wound management. The present work describes preparation of metronidazole/serratiopeptidase loaded alginate microspheres by emulsification method. In vitro characterizations like particle size analysis, % yield, % encapsulation, and in vitro release were carried out. Wound healing assessment was determined by physical, histological, and biochemical methods. Wound healing performance of experimental formulations was compared with marketed product in rabbits. Result obtained for alginate microspheres showed good loading efficiency with spherical in shape. Experimentation suggests wound healing is improved by using serratiopeptidase and metronidazole in full thickness wounds in rabbits.
Rath
G
G
Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India. goutamrath@rediffmail.com
Johal
E S
ES
Goyal
Amit K
AK
D000464:Alginates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002122:Calcium Chloride / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D003094:Collagen / Q000378:metabolism; D055808:Drug Discovery / Q000379:methods*; D004655:Emulsions; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008795:Metronidazole / Q000737:chemistry*; D008863:Microspheres; D009929:Organ Size / Q000187:drug effects; D010316:Particle Size; D010447:Peptide Hydrolases / Q000378:metabolism*; D011817:Rabbits; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
D000464:Alginates; D004655:Emulsions; D006603:Hexuronic Acids; D008795:Metronidazole; D020723:Glucuronic Acid; D003094:Collagen; D010447:Peptide Hydrolases; D002122:Calcium Chloride; C022640:serratiopeptidase