title: In vitro biomonitoring of contamination by estrogenic compounds in coastal environments: comments on the use of M. galloprovincialis.
abstract: The use of mussel extracts in in vitro bioassays which express the estrogen receptor could provide valuable information on the bioavailability of endocrine disruptors in coastal environments. The aim of this study was to assess the temporal variability of the estrogenic responses in bioassays in Mytilus galloprovincialis. A 6-month in situ experiment was conducted in order to follow the estrogenic activity on MELN cell line during the reproduction stages of mussels. Estradiol equivalents (EEQ) determined in mussels using the MELN cell lines ranged from 0.79 to 3.72 ng/g dry weight (d.w.) in males, from 0.42 to 2.33 ng/g d.w. in females and from 3.41 to 4.2 d.w. in undifferentiated bivalves. We observed an increase in EEQ values during the spawning stage for males, not for female. The maximal EEQ values were observed at the indifferent stage. We discuss these results in regards to the actual knowledge on mussels' reproductive cycle and to the possible impact of xeno-estrogens. Variations of E2 levels in mussels must be taken into account for further studies on xeno-estrogens monitoring using hER reporter cell-lines bioassays.
authors.affiliation: Hydrosciences Montpellier, Université Montpellier, Montpellier Cedex, France. david@msem.univ-montp2.fr
authors.forename: Arthur
authors.initials: A
authors.lastname: David
authors.forename: Hélène
authors.initials: H
authors.lastname: Fenet
authors.forename: Aurélie
authors.initials: A
authors.lastname: Escande
authors.forename: Dominique
authors.initials: D
authors.lastname: Munaron
authors.forename: David
authors.initials: D
authors.lastname: Rosain
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Maillot-Maréchal
authors.forename: Selim
authors.initials: S
authors.lastname: Aït-Aïssa
authors.forename: Claude
authors.initials: C
authors.lastname: Casellas
authors.forename: Elena
authors.initials: E
authors.lastname: Gomez
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D004958:Estradiol / Q000633:toxicity; D004967:Estrogens / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005602:France; D008297:Male; D049878:Mytilus / Q000187:drug effects* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D014874:Water Pollutants, Chemical; D004958:Estradiol