title: Block-copolymer-stabilized iodinated emulsions for use as CT contrast agents.
abstract: The objective of this study was to develop radiopaque iodinated emulsions for use as CT blood pool contrast agents. Three hydrophobic iodinated oils were synthesized based on the 2,3,5-triiodobenzoate moiety and formulated into emulsions using either phospholipids or amphiphilic polymers, i.e. Pluronic F68 and poly(butadiene)-b-poly(ethylene glycol) (PBD-PEO), as emulsifiers. The size, stability and cell viability was investigated for all stabilized emulsions. Three emulsions stabilized with either lipids or PBD-PEO were subsequently tested in vivo as a CT blood pool contrast agent in mice. While the lipid-stabilized emulsions turned out unstable in vivo, polymer-stabilized emulsions performed well in vivo. In blood, a contrast enhancement of 220 Hounsfield Units (HU) was measured directly after intravenous administration of 520 mg I/kg. The blood circulation half-life of a PBD-PEO stabilized emulsion was approximately 3 h and no noticeable in vivo toxicity was observed. These results show the potential of above emulsions for use as blood pool agents in contrast enhanced CT imaging.
authors.affiliation: Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands.
authors.forename: Anke
authors.initials: A
authors.lastname: de Vries
authors.forename: Erica
authors.initials: E
authors.lastname: Custers
authors.forename: Johan
authors.initials: J
authors.lastname: Lub
authors.forename: Sandra
authors.initials: S
authors.lastname: van den Bosch
authors.forename: Klaas
authors.initials: K
authors.lastname: Nicolay
authors.forename: Holger
authors.initials: H
authors.lastname: Grüll
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D003287:Contrast Media / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004655:Emulsions / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007455:Iodine / Q000737:chemistry / Q000494:pharmacology; D007459:Iodized Oil / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D003287:Contrast Media; D004655:Emulsions; D007459:Iodized Oil; D007455:Iodine