title: Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.
abstract: Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
authors.affiliation: Department of Cell and Organism Biology, Lund University, Lund, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Christoffersen
authors.forename: I
authors.initials: I
authors.lastname: Serra
authors.forename: M
authors.initials: M
authors.lastname: Terreni
authors.forename: J
authors.initials: J
authors.lastname: Piskur
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003016:Clostridium perfringens / Q000201:enzymology*; D016049:Didanosine / Q000138:chemical synthesis / Q000378:metabolism*; D010430:Pentosyltransferases / Q000378:metabolism*
chemical_list: D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D016049:Didanosine