Estimation of blood alcohol concentration by horizontal attenuated total reflectance-Fourier transform infrared spectroscopy.
Numerous methods like distillation followed by iodometric titrations, gas chromatograph (GC)-flame ionization detector, gas chromatograph-mass spectrophotometer, GC-Headspace, Breath analyzer, and biosensors including alcohol dehydrogenase (enzymatic) have been used to determine blood alcohol concentration (BAC). In the present study, horizontal attenuated total reflectance-Fourier transform infrared spectroscopy had been used to determine BAC in whole blood. The asymmetric stretching frequency of C-C-O group of ethanol in water (1,045 cm(-1)) had been used to calculate BAC using Beer's Law. A seven-point calibration curve of ethanol was drawn in the concentration range 24-790 mg dL(-1). The curve showed good linearity over the concentration range used (r(2)=0.999, standard deviation=0.0023). The method is accurate, reproducible, rapid, simple, and nondestructive in nature.
Sharma
Kakali
K
Department of Chemistry, Vivekananda Mahavidyalaya, Haripal, Hooghly, West Bengal, India.
Sharma
Shiba P
SP
Lahiri
Sujit C
SC
D000328:Adult; D000428:Alcohol Drinking / Q000097:blood*; D000431:Ethanol / Q000097:blood* / Q000737:chemistry; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data*; D014732:Vibration
D000431:Ethanol