title: Cyanide in bronchoalveolar lavage is not diagnostic for Pseudomonas aeruginosa in children with cystic fibrosis.
abstract: Early detection of the cyanobacterium Pseudomonas aeruginosa in the lungs of young children with cystic fibrosis (CF) is considered the key to delaying chronic pulmonary disease. We investigated whether cyanide in bronchoalveolar lavage (BAL) fluid could be used as an early diagnostic biomarker of infection. Cyanide was measured in 226 BAL samples (36 P. aeruginosa infected) obtained from 96 infants and young children with CF participating in an early surveillance programme involving annual BAL. Cyanide was detected in 97.2% of P. aeruginosa infected and 60.5% of uninfected samples. Cyanide concentrations were significantly higher in BALs infected with P. aeruginosa (median (25th-75th percentile) 27.3 (22.1-33.3) μM) than those which were not (17.2 (7.85-23.0) μM, p<0.001). The best sensitivity, specificity, positive and negative predictive values were obtained with a cut-off concentration of 20.6 μM, and were 83%, 66%, 32% and 96%, respectively. Neutrophil number in BAL was a significant predictor of cyanide concentration (p<0.001). Cyanide concentration can distinguish between P. aeruginosa infected and uninfected BALs as a group, but not individually; therefore, cyanide is a poor diagnostic biomarker of P. aeruginosa infection. Cyanide levels in BAL are related to the level of neutrophilic inflammation.
authors.affiliation: Division of Clinical Sciences, Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Stutz
authors.forename: C L
authors.initials: CL
authors.lastname: Gangell
authors.forename: L J
authors.initials: LJ
authors.lastname: Berry
authors.forename: L W
authors.initials: LW
authors.lastname: Garratt
authors.forename: B
authors.initials: B
authors.lastname: Sheil
authors.forename: P D
authors.initials: PD
authors.lastname: Sly
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018893:Bronchoalveolar Lavage; D002138:Calibration; D002648:Child; D002675:Child, Preschool; D003486:Cyanides / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D008137:Longitudinal Studies; D008171:Lung Diseases / Q000382:microbiology; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D009504:Neutrophils / Q000473:pathology; D011237:Predictive Value of Tests; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D003486:Cyanides