Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels.
We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Westerlund
Fredrik
F
Department of Physics, University of Gothenburg, Gothenburg, Sweden.
Persson
Fredrik
F
Kristensen
Anders
A
Tegenfeldt
Jonas O
JO
D010582:Bacteriophage lambda / Q000235:genetics; D001583:Benzoxazoles / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011806:Quinolinium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D001583:Benzoxazoles; D004279:DNA, Viral; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D012822:Silicon Dioxide; D004247:DNA