title: Sex steroid hormones in older men: longitudinal associations with 4.5-year change in hip bone mineral density--the osteoporotic fractures in men study.
abstract: BACKGROUND
  There is limited information on the association between sex hormones and bone loss in older men.
  
  
  OBJECTIVE
  Our objective was to determine the longitudinal association between sex steroid hormones and bone mineral density (BMD).
  
  
  METHODS
  We conducted a prospective study of 5995 men aged at least 65 yr old at six U.S. clinical centers.
  
  
  METHODS
  Sex steroid hormones were measured in a random sample of 1602 men. After exclusions, 1238 men were included in cross-sectional analyses and 969 in longitudinal analyses. Baseline sex hormones were measured using liquid chromatography-mass spectrometry. Bioavailable (Bio) estradiol (BioE2) and testosterone (BioT) were calculated from mass action equations. SHBG was measured using chemiluminescent substrate.
  
  
  METHODS
  BMD of the total hip, measured at baseline and once or twice afterward over 4.6 yr of follow-up, was evaluated.
  
  
  RESULTS
  The annualized percent change in hip BMD increased with decreasing BioE2 (P trend = 0.03). Men with the lowest BioE2 (<39.7 pmol/liter) compared with the highest BioE2 (> or =66.0 pmol/liter) experienced 38% faster rate of BMD loss (P < 0.05). There was no association between BioT and hip BMD loss. Men with lowest BioE2, lowest BioT, and highest SHBG experienced a 3-fold faster rate of BMD loss compared with men with higher levels (P = 0.02). A threshold effect of SHBG was observed; the rate of hip BMD loss increased in men with SHBG of 49-60 nM.
  
  
  CONCLUSIONS
  Low BioE2 and high SHBG levels were associated with lower BMD and faster hip BMD loss. The combination of low BioE2, low BioT, and high SHBG was associated with significantly faster rates of BMD loss.
authors.affiliation: University of Pittsburgh, Department of Epidemiology, 130 DeSoto Street, Crabtree A524, Pittsburgh, Pennsylvania 15261, USA. jcauley@edc.pitt.edu
authors.forename: Jane A
authors.initials: JA
authors.lastname: Cauley
authors.forename: Susan K
authors.initials: SK
authors.lastname: Ewing
authors.forename: Brent C
authors.initials: BC
authors.lastname: Taylor
authors.forename: Howard A
authors.initials: HA
authors.lastname: Fink
authors.forename: Kristine E
authors.initials: KE
authors.lastname: Ensrud
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Bauer
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Barrett-Connor
authors.forename: Lynn
authors.initials: L
authors.lastname: Marshall
authors.forename: Eric S
authors.initials: ES
authors.lastname: Orwoll
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D015519:Bone Density / Q000502:physiology; D003430:Cross-Sectional Studies; D050723:Fractures, Bone / Q000097:blood / Q000209:etiology; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006615:Hip; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D010024:Osteoporosis / Q000097:blood / Q000150:complications; D013997:Time Factors
chemical_list: D012739:Gonadal Steroid Hormones