Improved dsrA-based terminal restriction fragment length polymorphism analysis of sulfate-reducing bacteria.
To better describe the community structure of sulfate-reducing bacteria in environmental systems, we compared several dissimilatory sulfite reductase (dsr) primer sets for terminal restriction fragment length polymorphism application. A new reverse primer that increased allelic diversity estimates up to 5-fold was applied to hydrocarbon seep samples to examine the relationship between guild activity and diversity.
Santillano
Daniel
D
Microbial Habitat Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany.
Boetius
Antje
A
Ramette
Alban
A
D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004783:Environmental Microbiology; D010084:Oxidation-Reduction; D012150:Polymorphism, Restriction Fragment Length; D013431:Sulfates / Q000378:metabolism*
D017931:DNA Primers; D013431:Sulfates