title: Role of cholangiocyte bile Acid transporters in large bile duct injury after rat liver transplantation.
abstract: BACKGROUND
  The pathogenesis of nonanastomotic strictures with a patent hepatic artery remains to be investigated. This study focuses on the role of cholangiocyte bile acid transporters in bile duct injury after liver transplantation.
  
  
  METHODS
  Sprague-Dawley rats were divided into three groups (n=20 for each): the sham-operated group (Sham), the transplant group with 1-hr donor liver cold preservation (CP-1h), and the transplant group with 12-hr donor liver cold preservation (CP-12h). Bile was collected for biochemical analysis. The histopathologic evaluation of bile duct injury was performed and the cholangiocyte bile acid transporters apical sodium-dependent bile acid transporter (ASBT), ileal lipid binding protein (ILBP), and Ostalpha/Ostbeta were investigated. RESULTS.: The immunohistochemical assay suggested that ASBT and ILBP were expressed exclusively on large bile duct epithelial cells, whereas Ostalpha and Ostbeta were expressed on both small and large bile ducts. Western blot and quantitative polymerase chain reaction analysis showed that the expression levels of these transporters dramatically decreased after transplantation. It took seven to 14 days for ILBP, Ostalpha, and Ostbeta to recover, whereas ASBT recovered within 3 days and even reached a peak above the normal level seven days after operation. In the CP-12h group, the ratios of the ASBT/ILBP, ASBT/Ostalpha and ASBT/Ostbeta expression levels were correlated with the injury severity scores of large but not small bile ducts.
  
  
  CONCLUSIONS
  The results suggest that the unparallel alteration of cholangiocyte bile acid transporters may play a potential role in large bile duct injury after liver transplantation with prolonged donor liver preservation.
authors.affiliation: Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.forename: Lijin
authors.initials: L
authors.lastname: Zhao
authors.forename: Dajiang
authors.initials: D
authors.lastname: Li
authors.forename: Zipei
authors.initials: Z
authors.lastname: Liu
authors.forename: Geng
authors.initials: G
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Li
authors.forename: Shuguang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D001653:Bile Ducts, Intrahepatic / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D017931:DNA Primers; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation / Q000009:adverse effects / Q000473:pathology*; D008297:Male; D009926:Organ Preservation / Q000379:methods; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D027981:Symporters / Q000235:genetics / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D017931:DNA Primers; D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter; D012313:RNA