Proteomic analysis of two Trypanosoma cruzi zymodeme 3 strains.
Two Trypanosoma cruzi Z3 strains, designated as 3663 and 4167, were previously isolated from insect vectors captured in the Brazilian Amazon region. These strains exhibited different infection patterns in Vero, C6/36, RAW 264.7 and HEp-2 cell lineages, in which 3663 trypomastigote form was much less infective than 4167 ones. A proteomic approach was applied to investigate the differences in the global patterns of protein expression in these two Z3 strains. Two-dimensional (2D) protein maps were generated and certain spots were identified by mass spectrometry (MS). Our analyses revealed a significant difference in the expression profile of different proteins between strains 3663 and 4167. Among them, cruzipain, an important regulator of infectivity. This data was corroborated by flow cytometry analysis using anti-cruzipain antibody. This difference could contribute to the infectivity profiles observed for each strain by in vitro assay using different cell lines.
Kikuchi
Simone A
SA
Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil. sakemi@ioc.fiocruz.br
Sodré
Cátia L
CL
Kalume
Dário E
DE
Elias
Camila G R
CG
Santos
André L S
AL
de Nazaré Soeiro
Maria
M
Meuser
Marcus
M
Chapeaurouge
Alex
A
Perales
Jonas
J
Fernandes
Octavio
O
D000330:Aedes; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003546:Cysteine Endopeptidases / Q000032:analysis; D057056:Cysteine Proteases / Q000032:analysis; D048293:Didelphis / Q000469:parasitology; D015180:Electrophoresis, Gel, Two-Dimensional; D007303:Insect Vectors / Q000469:parasitology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D015800:Protozoan Proteins / Q000032:analysis*; D014225:Triatominae / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D014709:Vero Cells
D020543:Proteome; D015800:Protozoan Proteins; D057056:Cysteine Proteases; D003546:Cysteine Endopeptidases; C063214:cruzipain