title: Characterization of norovirus-associated traveler's diarrhea.
abstract: BACKGROUND
  Traveler's diarrhea is the most common medical complaint of international visitors to developing regions. Previous findings suggested that noroviruses (NoVs) are an underappreciated cause of traveler's diarrhea. METHODS. In the present study, we sought to define the presence of NoVs in 320 acute diarrheic stool samples collected from 299 US students who traveled to Guadalajara, Cuernavaca, or Puerto Vallarta, Mexico, during the period from 2007 through 2008. Conventional and quantitative real-time polymerase chain reaction assays were used to detect and determine NoV loads in stool samples. NoV strains were characterized by purification of viral RNA followed by sequencing of the viral capsid protein 1 gene. Sequences were compared using multiple sequence alignment, and phylogenetic trees were generated to evaluate the evolutionary relatedness of the viral strains associated with cases of traveler's diarrhea.
  
  
  RESULTS
  NoV RNA was detected in 30 (9.4%) of 320 samples. Twelve strains belonged to genogroup I, and 18 strains belonged to genogroup II. NoV prevalence was higher in the winter season than in the summer season (23% vs 7%, respectively; P = .001). The cDNA viral loads of genogroup I viruses were found to be 500-fold higher than those of genogroup II strains. Phylogenetic analysis revealed a diverse population of NoV strains over different locations and years.
  
  
  CONCLUSIONS
  NoV strains are important causes of traveler's diarrhea in Mexico, especially during the wintertime, and US students in Mexico may represent a suitable group for future NoV vaccine efficacy trials.
authors.affiliation: Department of Molecular Virology and Microbiology and Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: N
authors.initials: N
authors.lastname: Ajami
authors.forename: H
authors.initials: H
authors.lastname: Koo
authors.forename: C
authors.initials: C
authors.lastname: Darkoh
authors.forename: R L
authors.initials: RL
authors.lastname: Atmar
authors.forename: P C
authors.initials: PC
authors.lastname: Okhuysen
authors.forename: Z-D
authors.initials: ZD
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Flores
authors.forename: H L
authors.initials: HL
authors.lastname: Dupont
mesh_terms: D000328:Adult; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000473:pathology / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D017720:Molecular Epidemiology; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014195:Travel; D014481:United States; D019562:Viral Load; D055815:Young Adult
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral