title: Detection and genotyping of canine coronavirus RNA in diarrheic dogs in Japan.
abstract: To clarify the prevalence of canine coronavirus (CCoV) infection in Japan, faecal samples from 109 dogs with diarrhoea were examined for CCoV RNA together with canine parvovirus type 2 (CPV-2) DNA. The detection rates of CCoV and CPV-2 for dogs aged less than 1 year were 66.3% and 43.8%, while those for dogs aged 1 year or older were 6.9% and 10.3%, respectively, which were significantly different (p<0.0001 and p=0.0003, respectively), indicating not CPV-2 but CCoV is an important diarrhoea-causing organism in juvenile dogs. Among the CCoV-positive dogs, 65.5% and 72.7% showed to be positive for CCoV types I and II, respectively, and simultaneous detection rate of both types was high at 40.0%. Furthermore, transmissible gastroenteritis virus (TGEV)-like CCoV RNA was detected from 8 dogs. These findings indicate that CCoV type I and TGEV-like CCoV are already circulating in Japan, though no reports have been presented to date.
authors.affiliation: Veterinary Diagnostic Laboratory, Marupi Lifetech Co. Ltd., 103 Fushiocho, Ikeda, Osaka 563-0011, Japan. takehisa-soma@ds-pharma.co.jp
authors.forename: Takehisa
authors.initials: T
authors.lastname: Soma
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohinata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taharaguchi
authors.forename: Motonobu
authors.initials: M
authors.lastname: Hara
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000235:genetics*; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral