title: Monitoring biofilm development in a microfluidic device using modified confocal reflection microscopy.
abstract: The feasibility of a method to monitor biofilm development non-destructively in a microfluidic device was addressed. Here, we report that biofilm growth could be non-destructively monitored by an image analysis technique based on modification of confocal reflection microscopy.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennnoudai1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yawata
authors.forename: Kensuke
authors.initials: K
authors.lastname: Toda
authors.forename: Erika
authors.initials: E
authors.lastname: Setoyama
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Hiroo
authors.initials: H
authors.lastname: Uchiyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nomura
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors; D018929:Cell Culture Techniques; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D013295:Streptococcus mutans / Q000166:cytology* / Q000254:growth & development*