Kinetic hysteresis in collagen folding.
The triple helix of collagen shows a steep unfolding transition upon heating, whereas less steep and more gradual refolding is observed upon cooling. The shape of the hysteresis loop depends on the rate of temperature change as well as the peptide concentration. Experimental heating and cooling rates are usually much faster than rates of unfolding and refolding. In this work, collagen model peptides were used to study hysteresis quantitatively. Their unfolding and refolding profiles were recorded at different heating and cooling rates, and at different peptide concentrations. Data were fitted assuming kinetic mechanisms in which three chains combine to a helix with or without an intermediate that acts as a nucleus. A quantitative fit was achieved with the same kinetic model for the forward and backward reactions. Transitions of exogenously trimerized collagen models were also analyzed with a simplified kinetic mechanism. It follows that true equilibrium transitions can only be measured at high concentrations of polypeptide chains with slow scanning rates, for example, 0.1 degrees C/h at 0.25 mM peptide concentration of (Gly-Pro-Pro)(10). (Gly-Pro-4(R)Hyp)(10) folds approximately 2000 times faster than (Gly-Pro-Pro)(10). This was explained by a more stable nucleus, whereas the rate of propagation was almost equal. The analysis presented here can be used to derive kinetic and thermodynamic data for collagenous and other systems with kinetically controlled hysteresis.
Mizuno
Kazunori
K
Shriners Hospital for Children, Portland, Oregon, USA.
Boudko
Sergei P
SP
Engel
Jürgen
J
Bächinger
Hans Peter
HP
D000595:Amino Acid Sequence; D002151:Calorimetry; D003094:Collagen / Q000737:chemistry*; D006358:Hot Temperature; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary
D010446:Peptide Fragments; D003094:Collagen