[Application of turbulent flow chromatography in the analysis of biological samples].
The complexity of biological fluids makes the sample preparation necessary in the analytical process. The traditional techniques, such as liquid-liquid extraction and solid-phase extraction, are time-consuming and labor-intensive. Turbulent flow chromatography (TFC), as an on-line direct injection technique, possesses the advantages of reducing sample preparation steps and enabling the effective pre-concentration and clean-up of biological fluids. Therefore, it is a high-throughput and high-selectivity technique for biological sample pretreatment. In this review, the theory, advantages, characteristics and current status of TFC in bioanalysis are introduced and summarized.
Liu
Peng
P
Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.
Zhou
Jianliang
J
An
Jingjing
J
Li
Ping
P
D001774:Blood Chemical Analysis; D001826:Body Fluids / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods