Imaging and cell targeting characteristics of magnetic nanoparticles modified by a functionalizable zwitterionic polymer with adhesive 3,4-dihydroxyphenyl-l-alanine linkages.
Multifunctional magnetic nanoparticles (MNPs) modified by a zwitterionic polymer (pCBMA-DOPA(2)) containing one poly(carboxybetaine methacrylate) (pCBMA) chain and two 3,4-dihydroxyphenyl-L-alanine (DOPA) residue groups were developed. Results showed that MNPs modified by pCBMA were not only stable in complex media, but also provided abundant functional groups for ligand immobilization. The pCBMA-DOPA(2) MNPs had a hydrodynamic particle size of about 130 nm, a strong saturation magnetization of 110.2 emu/g Fe and a high transverse relaxivity of 428 mM(-1)s(-1). Long-term stability in phosphate-buffered saline (PBS) and 10% NaCl solution was achieved for over six months. Compared to MNPs coated with dextran, pCBMA-DOPA(2) MNPs presented better stability in 100% human blood serum at 37 degrees C. Macrophage cell uptake studies revealed that the uptake ratio of pCBMA-DOPA(2) MNPs was much lower than that of dextran MNPs. Furthermore, quantitative analysis results showed that after pCBMA-DOPA(2) MNPs were conjugated with a targeting RGD peptide, uptake by human umbilical vein endothelial cell (HUVEC) was notably increased, which was further visualized by magnetic resonance imaging (MRI).
Zhang
Lei
L
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, WA 98195, United States.
Xue
Hong
H
Gao
Changlu
C
Carr
Louisa
L
Wang
Jinnan
J
Chu
Baocheng
B
Jiang
Shaoyi
S
D000269:Adhesives; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival; D004295:Dihydroxyphenylalanine / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D042783:Endothelial Cells / Q000166:cytology; D005290:Ferric Compounds / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D044967:Serum / Q000378:metabolism
D000269:Adhesives; D005290:Ferric Compounds; D011108:Polymers; C000499:ferric oxide; D004295:Dihydroxyphenylalanine