title: Comparative transcriptional profiling-based identification of raphanusanin-inducible genes.
abstract: BACKGROUND
  Raphanusanin (Ra) is a light-induced growth inhibitor involved in the inhibition of hypocotyl growth in response to unilateral blue-light illumination in radish seedlings. Knowledge of the roles of Ra still remains elusive. To understand the roles of Ra and its functional coupling to light signalling, we constructed the Ra-induced gene library using the Suppression Subtractive Hybridisation (SSH) technique and present a comparative investigation of gene regulation in radish seedlings in response to short-term Ra and blue-light exposure.
  
  
  RESULTS
  The predicted gene ontology (GO) term revealed that 55% of the clones in the Ra-induced gene library were associated with genes involved in common defence mechanisms, including thirty four genes homologous to Arabidopsis genes implicated in R-gene-triggered resistance in the programmed cell death (PCD) pathway. Overall, the library was enriched with transporters, hydrolases, protein kinases, and signal transducers. The transcriptome analysis revealed that, among the fifty genes from various functional categories selected from 88 independent genes of the Ra-induced library, 44 genes were up-regulated and 4 were down-regulated. The comparative analysis showed that, among the transcriptional profiles of 33 highly Ra-inducible genes, 25 ESTs were commonly regulated by different intensities and duration of blue-light irradiation. The transcriptional profiles, coupled with the transcriptional regulation of early blue light, have provided the functional roles of many genes expected to be involved in the light-mediated defence mechanism.
  
  
  CONCLUSIONS
  This study is the first comprehensive survey of transcriptional regulation in response to Ra. The results described herein suggest a link between Ra and cellular defence and light signalling, and thereby contribute to further our understanding of how Ra is involved in light-mediated mechanisms of plant defence.
authors.affiliation: University of Tsukuba, Ibaraki, Japan.
authors.lastname: Moehninsi
authors.forename: Kenji
authors.initials: K
authors.lastname: Miura
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Nakajyo
authors.forename: Kosumi
authors.initials: K
authors.lastname: Yamada
authors.forename: Koji
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shigemori
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000528:radiation effects; D008027:Light; D005810:Multigene Family / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011759:Pyrrolidines / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D015398:Signal Transduction / Q000235:genetics; D013871:Thiones / Q000494:pharmacology*
chemical_list: D010937:Plant Growth Regulators; D011759:Pyrrolidines; D013871:Thiones; C414546:raphanusanin