title: Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.
abstract: Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.
authors.affiliation: Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Mithöfer
authors.forename: Bernd
authors.initials: B
authors.lastname: Müller
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.forename: Lutz A
authors.initials: LA
authors.lastname: Eichacker