[Characterization of the variable region within class 1 integrons in Klebsiella pneumoniae nosocomials strains].
BACKGROUND
Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria.


OBJECTIVE
To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae.


METHODS
Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation.


RESULTS
Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found.


CONCLUSIONS
The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.
Guzmán
Militza
M
Laboratorio de Bacteriología Molecular, Departamento de Bioanálisis, Universidad de Oriente, Cumaná, Estado Sucre, Venezuela. miltzaguz@cantv.net
Alonso
Guillermina
G
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial