title: Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.
abstract: The filamentous fungus Chrysosporium lucknowense (C1) is a rich source of cell wall degrading enzymes. In the present paper four arabinose releasing enzymes from C1 were characterized, among them one endoarabinanase, two arabinofuranosidases and one exoarabinanase. Combinations of these enzymes released up to 80% of the arabinose present in sugar beet arabinan to fermentable monosugars. Besides the main product arabinobiose, unknown arabinose oligomers are produced from highly branched arabinan when endoarabinanase was combined with exoarabinanase and/or arabinofuranosidase. All described arabinose releasing enzymes are temperature stable up to 50 degrees C and have a broad pH stability. This makes C1 arabinohydrolases suitable for many biotechnical applications, like co-fermentation bioethanol production.
authors.affiliation: Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Kühnel
authors.forename: S W A
authors.initials: SW
authors.lastname: Hinz
authors.forename: L
authors.initials: L
authors.lastname: Pouvreau
authors.forename: J
authors.initials: J
authors.lastname: Wery
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: H
authors.initials: H
authors.lastname: Gruppen
mesh_terms: D001089:Arabinose / Q000378:metabolism; D027461:Beta vulgaris / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002912:Chrysosporium / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D011134:Polysaccharides; C030080:araban; D001089:Arabinose; D006026:Glycoside Hydrolases