The effects of Tanshinone IIA on blood-brain barrier and brain edema after transient middle cerebral artery occlusion in rats.
Disruption of blood-brain barrier (BBB) and edema formation play a key role in the development of neurological dysfunction after cerebral ischemia. In this study, the effects of Tanshinone IIA (Tan IIA), one of the active ingredients of Salvia miltiorrhiza root, on the BBB and brain edema after transient middle cerebral artery occlusion in rats were examined. Our study demonstrated that Tan IIA reduced brain infarct area, water content in the ischemic hemisphere. Furthermore, Tan IIA significantly decreased BBB permeability to Evans blue, suppressed the expression of intercellular adhesion molecule-1 (ICAM-1), matrix metalloproteinase-9 (MMP-9), inhibited the degradation of tight junction proteins zonula occludens-1 (ZO-1) and Occludin. These results demonstrated that Tan IIA was effective for attenuating the extent of brain edema formation in response to ischemia injury in rats, partly by Tan IIA's protective effect on the BBB. Our results may have implications in the treatment of brain edema in cerebral ischemia.
Tang
Chao
C
Department of Neurosurgery, The General Hospital of Shenyang Military Region, No. 83, Wenhua Road, Shenhe District, Shen yang 110016, Liaoning Province, PR China. tangchao314159@yahoo.com.cn
Xue
Hongli
H
Bai
Changlin
C
Fu
Rong
R
Wu
Anhua
A
D045784:Abietanes; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004487:Edema / Q000517:prevention & control*; D005070:Evans Blue / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000188:drug therapy*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D062793:Occludin; D010539:Permeability; D010616:Phenanthrenes / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D019108:Tight Junctions / Q000378:metabolism; D014867:Water / Q000378:metabolism
D045784:Abietanes; D008565:Membrane Proteins; D062793:Occludin; C568987:Ocln protein, rat; D010616:Phenanthrenes; D010936:Plant Extracts; C021751:tanshinone; D014867:Water; D018799:Intercellular Adhesion Molecule-1; D005070:Evans Blue; D020780:Matrix Metalloproteinase 9