title: Activation of AMP-activated protein kinase on human gastric cancer cells by apoptosis induced by corosolic acid isolated from Weigela subsessilis.
abstract: Corosolic acid is one of the triterpenoids present in the leaves of Weigela subsessilis. The antidiabetic activity of corosolic acid has been reported previously, but to date, the anticancer effects on gastric cancer have been poorly studied. In this study, corosolic acid showed growth inhibition on SNU-601 human gastric cancer cells, with an IC₅₀ value of 16.9 ± 2.9 μM. Corosolic acid also triggered the activation of caspase-3 and poly (ADP-ribose) polymerase, while it was recovered by Z-VAD-FMK. Moreover, the cell growth/apoptosis activities of corosolic acid were regulated by the AMP-activated protein kinase-mammalian target of rapamycin (AMPK-mTOR) signals. These results showed that corosolic acid-mediated AMPK activation leads to inhibition of mTOR, thus providing a possible mechanism of action of corosolic acid in the inhibition of cancer cell growth and the induction of apoptosis.
authors.affiliation: Regional Cancer Institute, Chungnam National University Hospital, Daejeon, Korea.
authors.forename: Myung Sun
authors.initials: MS
authors.lastname: Lee
authors.forename: Chang Min
authors.initials: CM
authors.lastname: Lee
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Cha
authors.forename: Phuong Thien
authors.initials: PT
authors.lastname: Thuong
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: In Sang
authors.initials: IS
authors.lastname: Song
authors.forename: Seung Moo
authors.initials: SM
authors.lastname: Noh
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Sul
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D027922:Caprifoliaceae / Q000737:chemistry*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D018515:Plant Leaves / Q000737:chemistry; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C113861:corosolic acid; D011065:Poly(ADP-ribose) Polymerases; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; C505407:CASP3 protein, human; D053148:Caspase 3