[Human papillomavirus: prevalence and genotypes found among HIV-positive and negative women at a reference center in the far south of Brazil].
BACKGROUND
The aim of this study was detect the presence of human papillomavirus and investigate the prevalence and distribution of the HPV-6, 11, 16 and 18 genotypes among HIV-1 positive and negative women.


METHODS
Samples of cervical smears from 98 women were analyzed using the nested polymerase chain reaction for the presence of HPV and using the type-specific technique for genotype detection. Genotypes were confirmed by means of restriction fragment analysis. The chi-square and Fisher tests were performed for the statistical analysis.


RESULTS
HPV-DNA was observed in 66.3% of the samples analyzed: 76.4% in the HIV-positive group and 60% in the HIV-negative group (p = 0.1). Higher prevalence of viral infection due to oncogenic genotypes was observed in the HIV-positive group (65.2%) than in the HIV-negative group (28.6%), (p = 0.006), and HPV-16 was the most frequent in the two groups, followed by HPV-18.


CONCLUSIONS
It is suggested that HIV-positive women present a higher likelihood of infection by oncogenic HPV genotypes, and the importance of a screening and differential diagnosis program for this group is highlighted.
Entiauspe
Ludmila Gonçalves
LG
Laboratório de Biologia Molecular, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil. ludmila.entiauspe@gmail.com
Teixeira
Lisiane Ortiz
LO
Mendoza-Sassi
Raul Andrés
RA
Gonçalves
Carla Vitola
CV
Gonçalves
Paulo
P
Martinez
Ana Maria Barral de
AM
D000293:Adolescent; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D001938:Brazil; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000821:virology*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction; D014626:Vaginal Smears; D055815:Young Adult
D004279:DNA, Viral