Three-dimensional morphometry of strained bovine periodontal ligament using synchrotron radiation-based tomography.
The periodontal ligament (PDL) is a highly vascularized soft connective tissue. Previous studies suggest that the viscous component of the mechanical response may be explained by the deformation-induced collapse and expansion of internal voids (i.e. chiefly blood vessels) interacting with liquids (i.e. blood and interstitial fluids) flowing through the pores. In the present work we propose a methodology by means of which the morphology of the PDL vascular plexus can be monitored at different levels of compressive and tensile strains. To this end, 4-mm-diameter cylindrical specimens, comprising layers of bone, PDL and dentin covered by cementum, were strained at stretch ratios ranging from lambda = 0.6 to lambda = 1.4 and scanned using synchrotron radiation-based computer tomography. It was concluded that: (1) the PDL vascular network is layered in two distinct planes of blood vessels (BVs): an inner layer (close to the tooth), in which the BVs run in apico-coronal direction, and an outer layer (close to the alveolar bone), in which the BVs distribution is more diffuse; (2) during tension and compression, the porosity tissue is kept fairly constant; (3) mechanical straining induces important changes in BV diameters, possibly modifying the permeability of the PDL and thus contributing to the viscous component of the viscoelastic response observed under compressive forces.
Bergomi
Marzio
M
Laboratoire de mécanique appliquée et d'analyse de fiabilité, Ecole polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Cugnoni
Joël
J
Wiskott
H W Anselm
HW
Schneider
Philipp
P
Stampanoni
Marco
M
Botsis
John
J
Belser
Urs C
UC
D000818:Animals; D001808:Blood Vessels / Q000000981:diagnostic imaging / Q000502:physiology; D002417:Cattle; D008833:Microcirculation / Q000502:physiology; D010513:Periodontal Ligament / Q000033:anatomy & histology* / Q000098:blood supply / Q000000981:diagnostic imaging / Q000502:physiology; D016062:Porosity; D013048:Specimen Handling / Q000379:methods; D013314:Stress, Mechanical; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000379:methods; D014463:Ultrasonography