Improved molecular detection of Angiostrongylus cantonensis in mollusks and other environmental samples with a species-specific internal transcribed spacer 1-based TaqMan assay.
Angiostrongylus cantonensis is the most common cause of human eosinophilic meningitis. Humans become infected by ingesting food items contaminated with third-stage larvae that develop in mollusks. We report the development of a real-time PCR assay for the species-specific identification of A. cantonensis in mollusk tissue.
Qvarnstrom
Yvonne
Y
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30329-4018, USA.
da Silva
Ana Cristina Aramburu
AC
Teem
John L
JL
Hollingsworth
Robert
R
Bishop
Henry
H
Graeff-Teixeira
Carlos
C
da Silva
Alexandre J
AJ
D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D008969:Molecular Sequence Data; D008974:Mollusca / Q000469:parasitology*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer