title: Haplotypes that include the integrin alpha 11 gene are associated with tick burden in cattle.
abstract: BACKGROUND
  Infestations on cattle by the ectoparasite Boophilus (Rhipicephalus) microplus (cattle tick) impact negatively on animal production systems. Host resistance to tick infestation has a low to moderate heritability in the range 0.13 - 0.64 in Australia. Previous studies identified a QTL on bovine chromosome 10 (BTA10) linked to tick burden in cattle.
  
  
  RESULTS
  To confirm these associations, we collected genotypes of 17 SNP from BTA10, including three obtained by sequencing part of the ITGA11 (Integrin alpha 11) gene. Initially, we genotyped 1,055 dairy cattle for the 17 SNP, and then genotyped 557 Brahman and 216 Tropical Composite beef cattle for 11 of the 17 SNP. In total, 7 of the SNP were significantly (P < 0.05) associated with tick burden tested in any of the samples. One SNP, ss161109814, was significantly (P < 0.05) associated with tick burden in both the taurine and the Brahman sample, but the favourable allele was different. Haplotypes for three and for 10 SNP were more significantly (P < 0.001) associated with tick burden than SNP analysed individually. Some of the common haplotypes with the largest sample sizes explained between 1.3% and 1.5% of the residual variance in tick burden.
  
  
  CONCLUSIONS
  These analyses confirm the location of a QTL affecting tick burden on BTA10 and position it close to the ITGA11 gene. The presence of a significant association in such widely divergent animals suggests that further SNP discovery in this region to detect causal mutations would be warranted.
authors.affiliation: Cooperative Research Centre for Beef Genetic Technologies, University of New England, Armidale, NSW 2351, Australia.
authors.forename: Laercio R
authors.initials: LR
authors.lastname: Porto Neto
authors.forename: Rowan J
authors.initials: RJ
authors.lastname: Bunch
authors.forename: Blair E
authors.initials: BE
authors.lastname: Harrison
authors.forename: Kishore C
authors.initials: KC
authors.lastname: Prayaga
authors.forename: William
authors.initials: W
authors.lastname: Barendse
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology*; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics*; D039001:Integrin alpha Chains / Q000235:genetics*; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D048494:Rhipicephalus; D013984:Tick Infestations / Q000235:genetics* / Q000276:immunology
chemical_list: D039001:Integrin alpha Chains