title: Cochliones A-D, four new tetrahydrochromanone derivatives from endophytic Cochliobolus sp.
abstract: Four new tetrahydrochromanone derivatives, cochliones A-D (1-4), along with three known metabolites, 4-hydroxybenzaldehyde (5), 4-hydroxy-3-(3-methylbut-2-enyl) benzoic acid (6), and 2,2-dimethyl-2H-chromene-6-carboxylic acid (7), were characterized from the culture of Cochliobolus sp. IFB-E039, a fungus that resides inside the healthy root of Cynodon dactylon (Gramineae). The structures of 1-4 were accommodated by their spectral data (UV, IR, CD, HR-ESI-MS, (1)H and (13)C NMR, (1)H-(1)H COSY, HMQC, HMBC, and NOESY). The bioassay for the cytotoxic metabolite showed that cochlione C (3) inhibited human breast adenocarcinoma cell line (MCF-7) and human chronic myeloid leukemia cell line (K562) with IC(50) values of 21.99 and 4.59 microg/ml, respectively.
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Wang
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Ge
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Wu
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Song
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Tan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D002681:China; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D007624:KB Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000970:Antineoplastic Agents; D002867:Chromones