[NMDA-dependent increase of exracellular arginine level in the nucleus accumbens is mediated by NO-synthase activation].
In Sprague-Dawley rats, by means of in vivo microdialysis combined with HPLC analysis it was shown that intra-accumbal infusion of NMDA (10 microM, 100 microM, 1000 microM) dose-dependently increased the local dialysate level of arginine (an NO-synthase substrate). This increase was prevented by intraaccumbal infusion of 50 microM MK-801, an NMDA receptor antagonist, and it was significantly reduced by intra-accumbal infusion of 500 microM N-nitroarginine, an NO-synthase inhibitor. The data obtained indicate that extracellular level of arginine in the n. accumbens is regulated by NMDA receptors which act partially via NO-synthase activation.
Saul'skaia
N B
NB
Gorbachevskaia
A I
AI
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D008297:Male; D017551:Microdialysis; D016202:N-Methylaspartate / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019335:Nitroarginine / Q000494:pharmacology; D009714:Nucleus Accumbens / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism
D004791:Enzyme Inhibitors; D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D019335:Nitroarginine; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D001120:Arginine; D019001:Nitric Oxide Synthase