title: Y Chromosome microdeletion and altered sperm quality in human males with high concentration of seminal hexachlorocyclohexane (HCH).
abstract: Recent studies have shown Y chromosome microdeletions associated with male infertility. The factors responsible for Y chromosome microdeletions in spermatozoa remain unresolved. However, the environmental pollutants are known to damage DNA in differentiating and maturing germ cells in the male reproductive tract. Therefore, the aim of this study was to investigate the effects of seminal hexachlorocyclohexane (HCH) and its isomers, an environmental pollutant, in 50 fertile and 50 infertile males in relation to semen quality and the incidence of Y chromosome microdeletion in azoospermic factor (AZF) region. As compared to control, an increased HCH level and significantly decreased semen quality were observed in the infertile males. A positive significant association was found between sperm count with alpha-HCH and beta-HCH in the infertile males. A negative significant association was observed between sperm counts with gamma-HCH in asthenospermia patients and with beta-HCH and total HCH in oligo-asthenospermic patients. Out of 100 males studied, we found 10 patients with Yq deletion in AZFa and AZFc regions. Subdivision of infertile group revealed a deletion incidence of 61.5% in azoospermic patients, 11.1% in oligospermic patients and 16.6% in oligo-asthenospermic patients. The presence of Yq deletion in azoospermic patients with a significant mean difference of beta-HCH and total HCH in relation to reduced semen quality seem to corroborate with the mutagenic activity of HCH. The results of this study indicated the susceptibility of male germ line to mutagenic potential of HCH which is an acknowledged risk factor leading to spermatogenic failure.
authors.affiliation: Molecular Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow 226 007, India.
authors.forename: Faizan Haider
authors.initials: FH
authors.lastname: Khan
authors.forename: Panneer
authors.initials: P
authors.lastname: Ganesan
authors.forename: Sudhir
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D017353:Gene Deletion; D056426:Genetic Loci; D001556:Hexachlorocyclohexane / Q000032:analysis / Q000633:toxicity*; D006801:Humans; D007248:Infertility, Male / Q000139:chemically induced / Q000235:genetics; D007306:Insecticides / Q000032:analysis / Q000633:toxicity*; D007536:Isomerism; D008297:Male; D055101:Semen Analysis; D029589:Seminal Plasma Proteins / Q000235:genetics / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D029589:Seminal Plasma Proteins; C040534:alpha-hexachlorocyclohexane; D001556:Hexachlorocyclohexane; C023888:beta-hexachlorocyclohexane