Behavioural phenotyping of dopamine transporter knockdown mice using local small interference RNA.
The objective of this study was to evaluate basic behavioural phenotypes and methamphetamine-induced hyperactivity in dopamine transporter (DAT) knockdown and control mice after local injections of small interference RNA into ventral tegmental area/substantia nigra once every other day for 14 days. Local knockdown of DAT expression in the mouse brain did not affect basic behavioural functions, but these mice displayed elevated depression-like immobility and a approximately 35% reduction of methamphetamine-induced hyperactivity.
Chen
Yao-Chu
YC
Department of Psychology, National Taiwan University, Taipei, Taiwan.
Lai
Wen-Sung
WS
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism*; D055785:Gene Knockdown Techniques / Q000379:methods*; D006948:Hyperkinesis / Q000139:chemically induced*; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D010641:Phenotype; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D013378:Substantia Nigra / Q000378:metabolism / Q000502:physiology; D017557:Ventral Tegmental Area / Q000378:metabolism / Q000502:physiology
D050483:Dopamine Plasma Membrane Transport Proteins; D034741:RNA, Small Interfering; D008694:Methamphetamine