Measurement of intrinsic physiological membrane noise in cultured living cells.
An experimental technique and some preliminary observations are reported here for the measurement of electric noise and potentials intrinsic to the physiological function of living cells, using an in vitro yeast cells (Saccharomyces cerevisiae) model. The design and working of technique is based on a micro-electrode-based sensor working in a modified patch-clamp configuration. We present recordings of intrinsic noise and cellular electric potentials in living and aerobically respiring cells (in an electromagnetically shielded environment). An important observation of the effect of aerobic respiration on the studied cells is discussed, whereby conspicuously higher magnitude potentials were seen with aerobically respiring active yeast cells, as compared to anaerobic or dead cells. Recorded noise potentials from aerobically respiring cells are found to have a magnitude on the order of a few microVolts/cm and fall within the range of 140- in the low-frequency (LF) band.
Bukhari
Masroor H Shah
MH
Department of Physics, University of Houston, Houston, Texas, USA. mbukhari@gmail.com
Miller
John H
JH
D000968:Antimycin A / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D019069:Cell Respiration / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004553:Electric Conductivity; D000431:Ethanol / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D011190:Potassium Cyanide / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism
D012402:Rotenone; D000431:Ethanol; D000968:Antimycin A; D011190:Potassium Cyanide