title: MassSieve: panning MS/MS peptide data for proteins.
abstract: We present MassSieve, a Java-based platform for visualization and parsimony analysis of single and comparative LC-MS/MS database search engine results. The success of mass spectrometric peptide sequence assignment algorithms has led to the need for a tool to merge and evaluate the increasing data set sizes that result from LC-MS/MS-based shotgun proteomic experiments. MassSieve supports reports from multiple search engines with differing search characteristics, which can increase peptide sequence coverage and/or identify conflicting or ambiguous spectral assignments.
authors.affiliation: Laboratory of Neurotoxicology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA. slottad@ncbi.nlm.nih.gov
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Slotta
authors.forename: Melinda A
authors.initials: MA
authors.lastname: McFarland
authors.forename: Sanford P
authors.initials: SP
authors.lastname: Markey
mesh_terms: D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D057225:Data Mining / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D012984:Software; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014584:User-Computer Interface
chemical_list: D010446:Peptide Fragments