title: Limitation of the Cavitron technique by conifer pit aspiration.
abstract: The Cavitron technique facilitates time and material saving for vulnerability analysis. The use of rotors with small diameters leads to high water pressure gradients (DeltaP) across samples, which may cause pit aspiration in conifers. In this study, the effect of pit aspiration on Cavitron measurements was analysed and a modified 'conifer method' was tested which avoids critical (i.e. pit aspiration inducing) DeltaP. Four conifer species were used (Juniperus communis, Picea abies, Pinus sylvestris, and Larix decidua) for vulnerability analysis based on the standard Cavitron technique and the conifer method. In addition, DeltaP thresholds for pit aspiration were determined and water extraction curves were constructed. Vulnerability curves obtained with the standard method showed generally a less negative P for the induction of embolism than curves of the conifer method. Differences were species-specific with the smallest effects in Juniperus. Larix showed the most pronounced shifts in P(50) (pressure at 50% loss of conductivity) between the standard (-1.5 MPa) and the conifer (-3.5 MPa) methods. Pit aspiration occurred at the lowest DeltaP in Larix and at the highest in Juniperus. Accordingly, at a spinning velocity inducing P(50), DeltaP caused only a 4% loss of conductivity induced by pit aspiration in Juniperus, but about 60% in Larix. Water extraction curves were similar to vulnerability curves indicating that spinning itself did not affect pits. Conifer pit aspiration can have major influences on Cavitron measurements and lead to an overestimation of vulnerability thresholds when a small rotor is used. Thus, the conifer method presented here enables correct vulnerability analysis by avoiding artificial conductivity losses.
authors.affiliation: Institute of Botany, University of Innsbruck, Sternwartestrabetae 15, 6020 Innsbruck, Austria. barbara.beikircher@uibk.ac.at
authors.forename: B
authors.initials: B
authors.lastname: Beikircher
authors.forename: T
authors.initials: T
authors.lastname: Ameglio
authors.forename: H
authors.initials: H
authors.lastname: Cochard
authors.forename: S
authors.initials: S
authors.lastname: Mayr
mesh_terms: D002498:Centrifugation / Q000379:methods; D011312:Pressure; D064028:Tracheophyta / Q000502:physiology*; D014867:Water / Q000032:analysis* / Q000502:physiology
chemical_list: D014867:Water