title: [Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis].
abstract: OBJECTIVE
  To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.
  
  
  METHODS
  The content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.
  
  
  RESULTS
  Active ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).
  
  
  CONCLUSIONS
  Active ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.
authors.affiliation: Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Shunqin
authors.initials: S
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Luo
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D044950:Flavanones / Q000032:analysis* / Q000235:genetics / Q000528:radiation effects; D005419:Flavonoids / Q000032:analysis / Q000235:genetics / Q000494:pharmacology / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D008027:Light; D008516:Medicine, Chinese Traditional / Q000379:methods; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry / Q000254:growth & development*; D013535:Suspensions / Q000737:chemistry
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D013535:Suspensions; C038044:baicalin; C006680:baicalein