In vitro antiprotozoal, antimicrobial and antitumor activity of Pavetta crassipes K. Schum leaf extracts.
OBJECTIVE
To study the potential benefit of the traditional medicinal plant Pavetta crassipes K. Schum (Rubiaceae), which is widely distributed throughout West Africa, the methanol and dichloromethane extracts were isolated from the plant leaves to determine if they exhibited antiprotozoal, antibacterial, antifungal or antitumor activity in vitro.


METHODS
The methanol and dichloromethane extracts and their specific fractions were obtained using bioassay-guided fractionation and investigated for antiproliferative activity in vitro in microorganisms (Staphylococcus aureus, Escherichia coli and Candida albicans), protozoans (Trypanosoma cruzi, Trypanosoma brucei, Leishmania infantum and Plasmodium falciparum), and cancer (U373, PC3, MXT and A549) and normal cell lines (NHDF and MRC-5).


RESULTS
Most of the alkaloid fractions investigated exhibited antiproliferative activity in all the cancer cell lines, microorganisms and protozoans studied.


CONCLUSIONS
The benefit of Pavetta crassipes as a traditional medicinal remedy was confirmed using antiprotozoal and cytotoxicity assays in vitro. These analyses revealed that the components present in the alkaloid extract of Pavetta crassipes are responsible for its antiprotozoal and cytotoxic efficacy.
Baldé
Elhadj S
ES
Département de Pharmacie, Faculté de Médecine, Pharmacie et Odontostomatologie, Université de Conakry, Conakry, Guinea.
Megalizzi
Véronique
V
Traoré
Mohamed S
MS
Cos
Paul
P
Maes
Louis
L
Decaestecker
Christine
C
Pieters
Luc
L
Baldé
Aliou M
AM
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D016488:Medicine, African Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D019666:Rubiaceae / Q000737:chemistry*
D000470:Alkaloids; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000981:Antiprotozoal Agents; D010936:Plant Extracts