Characterization of and biomolecule immobilization on the biocompatible multi-walled carbon nanotubes generated by functionalization with polyamidoamine dendrimers.
Polyamidoamine (PAMAM) dendrimers were covalently tethered onto the surface of multi-walled carbon nanotubes (MWCNTs). The morphology and dispersive properties of the MWCNT-PAMAM hybrids were characterized and the peripheral functional groups were identified. Cytotoxicity to human osteosarcoma MG-63 cells, and protein and DNA immobilization ability of the hybrids were evaluated in detail. The dendrimers were present on the surface of MWCNTs in high density. The MWCNT-PAMAM hybrids exhibited good dispersibility and stability in aqueous solution. We showed that the hybrids are biocompatible, with no obvious cytotoxicity at concentration <25 microgmL(-1) over 24h. They possess excellent biomolecule-immobilization ability (bovine serum albumin and 5'-Fam-CAAggTCgTgTAAAggTCAg-3' were used as models). The functionalization of MWCNTs with PAMAM dendrimers improved the biomolecule-immobilization ability 70-fold and simultaneously decreased the cellular toxicity by about 30%. It is expected that the MWCNT-PAMAM hybrids will find promising applications in biosensors and biomolecule delivery systems in gene or protein therapy.
Zhang
Baoling
B
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Chen
Qiong
Q
Tang
Hao
H
Xie
Qingji
Q
Ma
Ming
M
Tan
Liang
L
Zhang
Youyu
Y
Yao
Shouzhuo
S
D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
D001672:Biocompatible Materials; D050091:Dendrimers; D037742:Nanotubes, Carbon; C104700:PAMAM Starburst; D012710:Serum Albumin, Bovine; D004247:DNA