title: Endogenous melatonin is not obligatory for the regulation of the rat sleep-wake cycle.
abstract: OBJECTIVE
  Though melatonin and melatonin receptor agonists are in clinical use and under development for treating insomnia, the role of endogenous melatonin in the regulation of the sleep-wake cycle remains uncertain. Some clinical case reports suggest that reduced nocturnal melatonin secretion is linked to sleep disruption, but pineal-gland removal in experimental animals has given variable results.
  
  
  METHODS
  The present study examined the effects of pinealectomy on the diurnal sleep-wake cycle of rats implanted with a radiotransmitter to allow continuous measurement of cortical electroencephalogram, electromyogram, and core temperature (Tc) without restraint in their home cages.
  
  
  RESULTS
  Tc was slightly (0.2 degrees C) but significantly lower after pineal removal. The total amount and diurnal distribution of locomotor activity, wake, non-rapid eye movement (NREM) sleep, and rapid eye movement (REM) sleep were unaltered in pinealectomized rats compared to sham-operated controls. Sleep consolidation measured by determining wake, NREM sleep, and REM sleep bout length and frequency was also unchanged. The EEG power spectrum during NREM sleep was unchanged, but a significant decrease in theta power (5-8 Hz) during REM sleep episodes was found.
  
  
  CONCLUSIONS
  Our data provide no evidence that endogenous circulating melatonin plays a role in regulating the sleep-wake cycle in rats. However, because cortical theta oscillations are generated in the CA1-3 layer of the hippocampus, neurons known to express melatonin receptors, this suggests that a lack of melatonin following pineal removal influences the function of these neurons and is consistent with previous work suggesting that endogenous melatonin is an important regulator of hippocampal physiology.
authors.affiliation: Division of Reproduction and Endocrinology, School of Biomedical and Health Sciences, King's College London, London, UK.
authors.forename: Simon P
authors.initials: SP
authors.lastname: Fisher
authors.forename: David
authors.initials: D
authors.lastname: Sugden
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000494:pharmacology* / Q000652:urine; D003404:Creatinine / Q000652:urine; D004569:Electroencephalography / Q000187:drug effects; D004576:Electromyography / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology* / Q000652:urine; D010870:Pineal Gland / Q000601:surgery; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000187:drug effects*; D012894:Sleep Stages / Q000187:drug effects; D014851:Wakefulness / Q000187:drug effects*
chemical_list: D002492:Central Nervous System Depressants; D003404:Creatinine; D008550:Melatonin