Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart.
A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.
Ouji
Yukiteru
Y
Program in Tissue Engineering, Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. oujix@naramed-u.ac.jp
Yoshikawa
Masahide
M
Moriya
Kei
K
Nishiofuku
Mariko
M
Ouji-Sageshima
Noriko
N
Matsuda
Ryosuke
R
Nishimura
Fumihiko
F
Ishizaka
Shigeaki
S
D000818:Animals; D002478:Cells, Cultured; D017364:Collagenases / Q000008:administration & dosage*; D006352:Heart Ventricles / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology*; D051379:Mice
D017364:Collagenases