Novel florfenicol and chloramphenicol resistance gene discovered in Alaskan soil by using functional metagenomics.
Functional metagenomics was used to search for florfenicol resistance genes in libraries of cloned DNA isolated from Alaskan soil. A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA. The predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.
Lang
Kevin S
KS
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
Anderson
Janet M
JM
Schwarz
Stefan
S
Williamson
Lynn
L
Handelsman
Jo
J
Singer
Randall S
RS
D000413:Alaska; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002702:Chloramphenicol Resistance; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D056186:Metagenomics / Q000379:methods*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C035534:florfenicol; D013839:Thiamphenicol