title: Cellular Behavior on Epidermal Growth Factor (EGF)-Immobilized PCL/Gelatin Nanofibrous Scaffolds.
abstract: Nano-scaled poly(ε-caprolactone) (PCL) and PCL/gelatin fibrous scaffolds with immobilized epidermal growth factor (EGF) were prepared for the purpose of wound-healing treatments. The tissue scaffolds were fabricated by electrospinning and the parameters that affect the electrospinning process were optimized. While the fiber diameters were 488 ± 114 nm and 663 ± 107 nm for PCL and PCL/gelatin scaffolds, respectively, the porosities were calculated as 79% for PCL and 68% for PCL/gelatin scaffolds. Electrospun PCL and PCL/gelatin scaffolds were first modified with 1,6-diaminohexane to introduce amino groups on their surfaces, then EGF was chemically conjugated to the surface of nanofibers. The results obtained from Attenuated Total Reflectance Fourier Transform Infrared (ATR-FT-IR) spectroscopy and quantitative measurements showed that EGF was successfully immobilized on nanofibrous scaffolds. L929 mouse fibroblastic cells were cultivated on both neat and EGF-immobilized PCL and PCL/gelatin scaffolds in order to investigate the effect of EGF on cell spreading and proliferation. According to the results, especially EGF-immobilized PCL/gelatin scaffolds exerted early cell spreading and superior and rapid proliferation compared to EGF-immobilized PCL scaffolds and neat PCL, PCL/gelatin scaffolds. Consequently, EGF-immobilized PCL/gelatin scaffolds could potentially be employed as novel scaffolds for skin tissueengineering applications.
authors.affiliation: a Hacettepe University, Chemical Engineering Department, 06800, Beytepe, Ankara, Turkey.
authors.forename: R Seda
authors.initials: RS
authors.lastname: Tığlı
authors.forename: N Merve
authors.initials: NM
authors.lastname: Kazaroğlu
authors.forename: Bora
authors.initials: B
authors.lastname: Mavış
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003959:Diamines / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D005780:Gelatin / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D020011:Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D003959:Diamines; D011091:Polyesters; D020011:Protective Agents; C016240:polycaprolactone; D004815:Epidermal Growth Factor; D005780:Gelatin; C007297:1,6-diaminohexane