[DJ-1 gene rearrangement mutation in patients with autosomal recessive early-onset parkinsonism using real-time PCR].
OBJECTIVE
To establish a technique platform of DJ-1 gene exon rearrangement using real-time PCR and to analyze DJ-1 gene exon rearrangement mutation in patients with autosomal recessive early-onset Parkinsonism(AREP).


METHODS
Real-time PCR was used to analyze DJ-1 gene exon rearrangement mutation in 22 probands with AREP from unrelated Chinese Han families and 30 normal controls.


RESULTS
We obtained satisfactory real-time PCR reaction conditions and primers of DJ-1 gene coding exons No exon rearrangement mutation in the DJ-1 gene is detected in this group.


CONCLUSIONS
We established platform of DJ-1 gene exon rearrangement using real-time PCR. Exon rearrangement mutation in the DJ-1 gene is rare in Chinese patients with AREP.
Zhang
Hainan
H
Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Xiao
Bin
B
Nie
Liluo
L
Guo
Jifeng
J
Wang
Chunyu
C
Wang
Lei
L
He
Dan
D
Yan
Xinxiang
X
Tang
Beisha
B
D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D002648:Child; D002675:Child, Preschool; D002681:China / Q000208:ethnology; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D009154:Mutation; D015513:Oncogene Proteins / Q000235:genetics*; D020734:Parkinsonian Disorders / Q000235:genetics*; D000071617:Protein Deglycase DJ-1; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1