title: Improved molecular detection of Angiostrongylus cantonensis in mollusks and other environmental samples with a species-specific internal transcribed spacer 1-based TaqMan assay.
abstract: Angiostrongylus cantonensis is the most common cause of human eosinophilic meningitis. Humans become infected by ingesting food items contaminated with third-stage larvae that develop in mollusks. We report the development of a real-time PCR assay for the species-specific identification of A. cantonensis in mollusk tissue.
authors.affiliation: Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30329-4018, USA.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Qvarnstrom
authors.forename: Ana Cristina Aramburu
authors.initials: AC
authors.lastname: da Silva
authors.forename: John L
authors.initials: JL
authors.lastname: Teem
authors.forename: Robert
authors.initials: R
authors.lastname: Hollingsworth
authors.forename: Henry
authors.initials: H
authors.lastname: Bishop
authors.forename: Carlos
authors.initials: C
authors.lastname: Graeff-Teixeira
authors.forename: Alexandre J
authors.initials: AJ
authors.lastname: da Silva
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D008969:Molecular Sequence Data; D008974:Mollusca / Q000469:parasitology*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer