KIOM-79 prevents xylose-induced lens opacity and inhibits TGF-beta2 in human lens epithelial cells cultured under high glucose.
OBJECTIVE
To investigate the effects of KIOM-79 in preventing the development of diabetic complications, such as cataracts.


METHODS
The inhibitory effects of KIOM-79 were assessed in a model of xylose-induced lens opacity and on changes mediated by high levels of glucose in human lens epithelial (HLE-B3) cells.


RESULTS
In lenses treated with KIOM-79, opacity was significantly improved and glutathione (GSH) was increased compared to controls. In HLE-B3 cells treated with KIOM-79, high glucose-mediated increases in TGF-beta2, alphaB-crystallin, and fibronectin were significantly inhibited in a dose-dependent manner. KIOM-79 decreased the phosphorylation of p-Smad2/3, pp38MAPK, pp44/42, and NF-kappaB signaling in cells grown under high glucose conditions.


CONCLUSIONS
KIOM-79 is protective against lens opacity and protects HLE-B3 cells from the toxic effects of high glucose. Therefore, KIOM-79 may provide a potential therapeutic approach for preventing diabetic complications, such as cataracts.
Kim
Nan Hee
NH
Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon, Republic of Korea.
Kim
Young Sook
YS
Jung
Dong Ho
DH
Kim
Jin Sook
JS
D002460:Cell Line; D003318:Corneal Opacity / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D053781:Transforming Growth Factor beta2 / Q000037:antagonists & inhibitors*; D014994:Xylose / Q000633:toxicity
C517978:KIOM 79; D010936:Plant Extracts; D020011:Protective Agents; D053781:Transforming Growth Factor beta2; D014994:Xylose; D005978:Glutathione; D005947:Glucose