title: [Exploiting bioactive Enediynes from marine microbe based on activity and gene screening].
abstract: OBJECTIVE
  A high quality library of marine microbes and their associated natural products is critical for successful drug discovery. In this research a method to assess the quality of our marine microbial natural product library through screening for novel enediyne-like compounds from this library was set up.
  
  
  METHODS
  A high throughput screening assay based on the unique DNA-damage activity of enediyne-like compounds has been constructed and our marine microbial natural product library was screened. Because the polyketide synthase responsible for the biosynthesis of enediyne core is a conserved iterative type I polyketide synthase, a sequence-based screening method was built to get the strains that harbored enediyne biosynthesis gene clusters.
  
  
  RESULTS
  Through activity-based screening, a positive hit from our marine natural product library was acquired. 16S rRNA analysis has revealed that this strain-LS481 was 99% similar to Micromonospora chersina DSM 44151T that could produce enediyne compound-Dynemicins. The crude extract of LS481 showed similar characteristics with Dynemicin A. In addition, two strains, MS098 and LS2004, were acquired through sequence-based screening. The 16S rRNA of MS098 was 100% the same as Streptomyces griseus NBRC 13350, and LS2004 was most close to Streptomyces vinaceus NBRC 13425? and Streptomyces cirratus NRRL B-3250T.
  
  
  CONCLUSIONS
  We can successfully isolate enediyne compounds through the activity-based screening and assess the potential of producing enediyne compounds through sequence-based screening.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Pei
authors.forename: Huanqin
authors.initials: H
authors.lastname: Dai
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Liu
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D053281:Enediynes / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000187:drug effects; D008386:Marine Biology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000187:drug effects* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D053281:Enediynes