Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.
Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation. In this condition, nattokinase activity was 630 unit/ml at 12 h. In batch culture of B. subtilis in fermentor, the highest nattokinase activity of 3400 unit/ml was obtained at 10h with 50 g/L of peptone supplementation. From the batch kinetics data, it was shown that nattokinase production was growth-associated and culture should be harvested before stationary phase for maximum nattokinase production. In fed-batch culture of B. subtilis using pH-stat feeding strategy, cell growth (optical density monitored at 600 nm) increased to ca. 100 at 22 h, which was 2.5 times higher than that in batch culture. The highest nattokinase activity was 7100 unit/ml at 19 h, which was also 2.1 times higher than that in batch culture.
Cho
Young-Han
YH
Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Song
Jae Yong
JY
Kim
Kyung Mi
KM
Kim
Mi Kyoung
MK
Lee
In Young
IY
Kim
Sang Bum
SB
Kim
Hyeon Shup
HS
Han
Nam Soo
NS
Lee
Bong Hee
BH
Kim
Beom Soo
BS
D001412:Bacillus subtilis / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D013381:Subtilisins / Q000096:biosynthesis*; D013997:Time Factors
D003470:Culture Media; D013381:Subtilisins; C053771:nattokinase