Immunoenhancing effects of Montanide ISA oil-based adjuvants on recombinant coccidia antigen vaccination against Eimeria acervulina infection.
The current study was conducted to investigate the immunoenhancing effects of Montanide adjuvants on protein subunit vaccination against avian coccidiosis. Broiler chickens were immunized subcutaneously with a purified Eimeria acervulina recombinant profilin protein, either alone or mixed with one of four adjuvants (ISA 70 VG, ISA 71 VG, ISA 201 VG or ISA 206 VG), and body weight gains, fecal oocyst shedding, and humoral and innate immune responses were evaluated following oral challenge infection with live E. acervulina oocysts. Immunization with profilin plus ISA 70 VG or ISA 71 VG increased body weight gains compared with vaccination with profilin alone. Profilin plus ISA 71 VG also reduced fecal oocyst shedding compared with vaccination in the absence of adjuvant. All adjuvants enhanced profilin serum antibody titers. Increased levels of gene transcripts encoding IL-2, IL-10, IL-17A, and IFN-gamma, but decreased levels of IL-15 mRNAs, were seen in intestinal intraepithelial lymphocytes of chickens immunized with profilin plus adjuvants compared with immunization with profilin alone. Finally, increased infiltration of lymphocytes, especially CD8(+) lymphocytes at the site of immunization was observed in birds given profilin plus ISA 71 VG compared with profilin alone. These results demonstrate that vaccination with the E. acervulina profilin subunit vaccine in combination with Montanide adjuvants enhances protective immunity against avian coccidiosis.
Jang
Seung I
SI
Animal and Natural Resources Institute, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Lillehoj
Hyun S
HS
Lee
Sung Hyen
SH
Lee
Kyung Woo
KW
Park
Myeong Seon
MS
Bauchan
Gary R
GR
Lillehoj
Erik P
EP
Bertrand
François
F
Dupuis
Laurent
L
Deville
Sebastien
S
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D001835:Body Weight / Q000187:drug effects; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D005243:Feces / Q000469:parasitology; D005786:Gene Expression Regulation / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects*; D007113:Immunity, Innate / Q000187:drug effects*; D008214:Lymphocytes; D008353:Mannitol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D009829:Oleic Acids / Q000008:administration & dosage / Q000494:pharmacology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D051304:Profilins / Q000276:immunology / Q000494:pharmacology; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D009829:Oleic Acids; D051304:Profilins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C477385:montanide ISA 51; D008353:Mannitol