Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line.
Haematophagous arthropods are the primary vectors in the transmission of Rickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between Ixodes scapularis histone H2B and Rickettsia felis. Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B with R. felis proteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediating R. felis internalization into ISE6 cells.
Thepparit
Chutima
C
Department of Pathobiological Sciences, Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803, USA.
Bourchookarn
Apichai
A
Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand.
Petchampai
Natthida
N
Department of Pathobiological Sciences, Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803, USA.
Barker
Steven A
SA
Department of Comparative Biomedical Sciences, Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803, USA.
Macaluso
Kevin R
KR
Department of Pathobiological Sciences, Louisiana State University, School of Veterinary Medicine, Baton Rouge, LA 70803, USA.
D000818:Animals; D001096:Arachnid Vectors / Q000378:metabolism* / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D018884:Ixodes / Q000378:metabolism* / Q000382:microbiology; D011485:Protein Binding; D041161:Rickettsia felis / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D006657:Histones