title: Anti-inflammatory, anti-angiogenic, and anti-nociceptive activities of the chloroform fraction of a methanol extract from Rosa davurica Pall. leaves in experimental animal models.
abstract: Rosa davurica Pall. has been traditionally used to treat inflammatory diseases and tumors. Its dried leaves were extracted with absolute methanol, and the methanol extract was successively fractionated into n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. Anti-angiogenic and anti-nociceptive activities were determined using the chick embryo chorioallantoic membrane (CAM) assay and acetic acid-induced writhing response, respectively. Anti-inflammatory activity was evaluated using two in vivo mouse models, acetic acid-induced vascular permeability and carrageenan-induced inflammation in the air-pouch. The methanol extract gave rise to significant inhibition in the CAM angiogenesis, and showed marked 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Among the fractions prepared from the methanol extract, the chloroform fraction exhibited highest inhibitory effect in the CAM angiogenesis. The chloroform fraction displayed anti-inflammatory activities in vascular permeability and air-pouch models. In the air-pouch model, it was able to diminish exudate volume, number of polymorphonulcear leukocytes, and nitrite content. It also showed anti-nociceptive activity in the writhing response model in mice. The leaves of R. davurica possess anti-angiogenic and related anti-inflammatory and anti-nociceptive activities, which would provide some therapeutic support on its traditional use.
authors.affiliation: Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Jung
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Sa
authors.forename: Yun Seon
authors.initials: YS
authors.lastname: Song
authors.forename: Tae-Heum
authors.initials: TH
authors.lastname: Shim
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Park
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects; D002642:Chick Embryo; D002725:Chloroform / Q000737:chemistry; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005609:Free Radicals / Q000737:chemistry; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000188:drug therapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D027826:Rosa / Q000737:chemistry*
chemical_list: D000700:Analgesics; D020533:Angiogenesis Inhibitors; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D002725:Chloroform; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol