title: Anti-Heat Shock Protein 70 antibody levels are increased in myasthenia gravis and Guillain-Barré syndrome.
abstract: Myasthenia gravis (MG) is an autoimmune disorder where patients develop autoantibodies towards skeletal muscle proteins (e.g. acetylcholine receptor and muscle specific kinase), causing weakness in striated muscles. Ocular MG (OMG) represents a subtype of (MG) affecting only the periocular muscles. The pathogenesis of this phenotype remains unclear. Heat Shock Protein 70 (Hsp70) plays a role in immune regulation. Antibodies against this protein are associated with several autoimmune diseases, and its biological significance has been shown in vivo. We have therefore examined the concentration of anti-Hsp70 antibodies in sera from 35 OMG patients and 94 patients with generalized MG (GMG) using ELISA assays. The antibody concentrations were compared to those in patients with multiple sclerosis (MS), Guillain-Barré syndrome (GBS) and to healthy controls. MG patients had significantly higher anti-Hsp70 antibody concentrations than both MS patients and healthy controls. GBS patients had higher antibody levels than all other groups. No difference in antibody levels was found when comparing OMG and GMG. Our results suggest that patients with MG and GBS have a previous or current increased exposure to Hsp70 antigens. The similarity between GMG and OMG strengthens the hypothesis that OMG represents a systemic disease, similar to GMG.
authors.affiliation: Department of Clinical Medicine, University of Bergen, Bergen, Norway. geir.helgeland@gmail.com
authors.forename: Geir
authors.initials: G
authors.lastname: Helgeland
authors.forename: Axel
authors.initials: A
authors.lastname: Petzold
authors.forename: Jana Midelfart
authors.initials: JM
authors.lastname: Hoff
authors.forename: Nils Erik
authors.initials: NE
authors.lastname: Gilhus
authors.forename: Gordon T
authors.initials: GT
authors.lastname: Plant
authors.forename: Fredrik Robert
authors.initials: FR
authors.lastname: Romi
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies / Q000097:blood* / Q000145:classification; D001323:Autoantibodies / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020275:Guillain-Barre Syndrome / Q000097:blood* / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009157:Myasthenia Gravis / Q000097:blood* / Q000276:immunology*; D013223:Statistics as Topic; D018709:Statistics, Nonparametric
chemical_list: D000906:Antibodies; D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins