Isolation and characterization of a nitrosoguanidine-induced Enterococcus faecium MTCC 5153 mutant defective in enterocin biosynthesis.
Enterocin A (EntA) is a low molecular weight, heat-stable, chromosomally encoded class IIa bacteriocin produced by several strains of Enterococcus faecium. In this study, a mutated strain of E. faecium MTCC 5153 was characterized for its sensitivity to EntA, immunity function and for the production of induction factor. Nucleotide sequencing of the putative promoter of the entA operon suggested point mutations upstream of the entA gene. The mutant was sensitive to several class IIa bacteriocins and was found to adsorb 20% more bacteriocin compared to its wild-type counterpart.
Halami
Prakash M
PM
Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570 020, Council of Scientific and Industrial Research, India. prakashalami@cftri.res.in
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D004251:DNA Transposable Elements; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009604:Nitrosoguanidines / Q000494:pharmacology; D009876:Operon; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D004251:DNA Transposable Elements; D009604:Nitrosoguanidines; C107799:enterocin A