Interspecific hybrids of Hordeum marinum ssp. marinum x H. bulbosum are mitotically stable and reveal no gross alterations in chromatin properties.
Interspecific hybrids between the related wild barley species Hordeum marinum and H. bulbosum were generated and tested regarding their chromosomal stability and chromatin properties. Unlike in H. vulgare x H. bulbosum hybrid embryos, there was no effect of temperature on uniparental chromosome elimination or retention during hybrid seed development and 7 chromosomes from each parent were detected according to genomic in situ hybridization analyses. The centromere-specific histone H3 gene (CENH3) of both parental genomes is active in hybrid plants. Indirect immunostaining of flow-sorted 2C nuclei indicated that no major reorganization of histone H3 methylation (K4, K9 and K27), H3K9 acetylation and histone H2A ubiquitination marks or location of active RNA polymerase II sites occurred after interspecific hybridization.
Sanei
M
M
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Pickering
R
R
Fuchs
J
J
Banaei Moghaddam
A M
AM
Dziurlikowska
A
A
Houben
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D023902:Chromosome Pairing / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D044127:Epigenesis, Genetic; D017343:Genes, Plant; D042822:Genomic Instability; D006657:Histones / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D002843:Chromatin; D017931:DNA Primers; D018744:DNA, Plant; D006657:Histones; D010940:Plant Proteins; D012319:RNA Polymerase II