title: Acquisition of a transposon encoding extended-spectrum beta-lactamase SHV-12 by Pseudomonas aeruginosa isolates during the clinical course of a burn patient.
abstract: Three of seven clonally related Pseudomonas aeruginosa strains isolated from a burn patient produced the extended-spectrum beta-lactamase (ESBL) SHV-12. Its gene was flanked by two IS26 elements with a large transposon (>24 kb). The transposon also contained at least five IS26 elements and a gene encoding the amikacin resistance determinant aminoglycoside 6'-N-acetyltransferase type Ib [aac(6')-Ib]. It was inserted into the gene PA5317 in the P. aeruginosa chromosome.
authors.affiliation: Department of Traumatology and Critical Care Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan. uemura@sapmed.ac.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Uemura
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Yokota
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Mizuno
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakawaki
authors.forename: Keigo
authors.initials: K
authors.lastname: Sawamoto
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Maekawa
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Tanno
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Mori
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Asai
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000328:Adult; D002056:Burns / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases