title: Poly(vinylpyrrolidone-b-styrene) block copolymers tethered surfaces for protein adsorption and cell adhesion regulation.
abstract: Poly(vinylpyrrolidone-b-styrene) (PVP-b-PS) diblock copolymers tethered to glass surfaces were prepared, and the effects on protein adsorption and cellular behavior to the glass and the modified glass surfaces investigated. The PVP-b-PS grafting process was confirmed by water contact angle and XPS measurements. The results obtained for the water contact angles suggest that there are two phases that coexist on the PVP-b-PS block copolymer tethered surface, under aqueous conditions. Although the PVP-b-PS surface possessed, to some extent, a protein resistant property, following introduction of the PS segment to the end of tethered PVP, both fibrinogen and lysozyme adsorption were increased significantly. The PVP-b-PS modified surface, based on Western-blot analysis, appeared to have the greatest amount of surface bound vitronectin, however the conformation of the adsorbed vitronectin may have subsequently been affected by the surface tethered copolymer as was suggested by cell culture results. From these results, we proposed that protein adsorption and cell adhesion can be regulated by tuning the chemical compositions of diblock copolymers tethered to surfaces.
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Rd., Wuhan 430070, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D002645:Chickens; D005340:Fibrinogen / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D005898:Glass; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology*; D011205:Povidone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D019096:Vitronectin / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D011137:Polystyrenes; D019096:Vitronectin; C552733:poly(vinylpyrrolidone-b-styrene); D014867:Water; D005340:Fibrinogen; C421864:hen egg lysozyme; D009113:Muramidase; D011205:Povidone