Bidirectional immobilization of affinity-tagged cytochrome c on electrode surfaces.
Here, we report a new strategy for the directed bivalent immobilization of cyt c on or between gold electrodes. C-terminal modification with cys- or his-tag did not affect the functional integrity of the protein. In combination with electrostatic protein binding, these tags enable a bifunctional immobilization between two electrodes or alternatively one electrode and interacting enzymes.
Schröper
Florian
F
Institute of Bio- and Nanosystems-2, Research Center Jülich, JARA-Fundamentals of Future Information Technology, 52425 Jülich, Germany.
Baumann
Arnd
A
Offenhäusser
Andreas
A
Mayer
Dirk
D
D000595:Amino Acid Sequence; D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
D004800:Enzymes, Immobilized; D006046:Gold; D045304:Cytochromes c