Site-specific immobilization of DNA in glass microchannels via photolithography.
For the first time, a microchannel was photochemically patterned with a functional linker. This simple method was developed for the site-specific attachment of DNA via this linker onto silicon oxide surfaces (e.g., fused silica and borosilicate glass), both onto a flat surface and onto the inside of a fused silica microchannel. Sharp boundaries in the micrometer range between modified and unmodified zones were demonstrated by the attachment of fluorescently labeled DNA oligomers. Studies of repeated hybridization-dehybridization cycles revealed selective and reversible binding of cDNA strands at the explicit locations. On average, approximately 7 x 10(11) fluorescently labeled DNA molecules were hybridized per square centimeter. The modified surfaces were characterized with X-ray photoelectron spectroscopy, infrared microscopy, static contact angle measurements, confocal laser scanning microscopy, and fluorescence detection (to quantify the attachment of the fluorescently labeled DNA).
Vong
Tuha
T
Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands.
ter Maat
Jurjen
J
van Beek
Teris A
TA
van Lagen
Barend
B
Giesbers
Marcel
M
van Hest
Jan C M
JC
Zuilhof
Han
H
D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D005898:Glass; D046228:Microarray Analysis / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D055668:Photochemical Processes; D013499:Surface Properties
D005456:Fluorescent Dyes; D004247:DNA