Rapid protein production using CHO stable transfection pools.
During early preclinical development of therapeutic proteins, representative materials are often required for process development, such as for pharmacokinetic/pharmacodynamic studies in animals, formulation design, and analytical assay development. To rapidly generate large amounts of representative materials, transient transfection is commonly used. Because of the typical low yields with transient transfection, especially in CHO cells, here we describe an alternative strategy using stable transfection pool technology. Using stable transfection pools, gram quantities of monoclonal antibody (Mab) can be generated within 2 months post-transfection. Expression levels for monoclonal antibodies can be achieved ranging from 100 mg/L to over 1000 mg/L. This methodology was successfully scaled up to a 200 L scale using disposable bioreactor technology for ease of rapid implementation. When fluorescence-activated cell sorting was implemented to enrich the transfection pools for high producers, the productivity could be improved by about three-fold. We also found that an optimal production time window exists to achieve the highest yield because the transfection pools were not stable and productivity generally decreased over length in culture. The introduction of Universal chromatin-opening elements elements into the expression vectors led to significant productivity improvement. The glycan distribution of the Mab product generated from the stable transfection pools was comparable to that from the clonal stable cell lines.
Ye
Jianxin
J
Merck & Co., Inc., Bioprocess Research and Development, Rahway, NJ 07065, USA. jianxin_ye@merck.com
Alvin
Krista
K
Latif
Haythem
H
Hsu
Amy
A
Parikh
Vanessa
V
Whitmer
Travis
T
Tellers
Melanie
M
de la Cruz Edmonds
M Celina
MC
Ly
Jeffrey
J
Salmon
Peter
P
Markusen
Julia F
JF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D006031:Glycosylation; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D000911:Antibodies, Monoclonal; D011506:Proteins