Influence of endothelium on beta-adrenoceptor-mediated mechanical and electrical functions in rat pulmonary artery.
The effect of isoprenaline on mechanical and electrical functions was studied in intact and denuded rat isolated pulmonary arteries. In intact blood vessels, isoprenaline-induced relaxation was significantly attenuated by acidification of buffer, presence of Ba(2+) and/or ouabain. Isoprenaline produced hyperpolarisation of vascular muscle cells increasing E(m) from -61.0+/-0.44 (n=190 cells) to -70.0+/-0.74 mV (n=31 cells; mean+/-SE). The latter effect was inhibited by acidification of buffer, tetraethylammonium (TEA), Ba(2+) and/or ouabain. Isoprenaline-mediated relaxation was also significantly inhibited by the removal of endothelial cells. Acidification of buffer, or the presence of Ba(2+), or ouabain alone did not result in further inhibition of relaxation to isoprenaline in denuded tissues. However, Ba(2+) and ouabain combined caused further inhibition of the relaxant responses to isoprenaline in denuded tissues analogous to intact tissue. Combined Ba(2+), TEA and ouabain also caused substantial inhibition of relaxant response to isoprenaline. Inclusion of Ba(2+), TEA or ouabain in acidic buffer did not further inhibit relaxation to isoprenaline when compared to Ba(2+), TEA and ouabain combined in regular buffer. Denudation of blood vessels resulted in a significant hyperpolarisation of vascular muscle (-67.4+/-0.35 mV; n=195 cells) due to the activation of K channels and Na(+)/K(+)-ATPase. In denuded tissue, isoprenaline was unable to further increase E(m) (-68.8+/-0.44 mV n=36). Isoprenaline-induced hyperpolarisation involves activation of K channels and Na(+)/K(+)-ATPase of smooth muscle cells possibly in parallel but mutually dependent on the presence of endothelial cells.
Mahajan
Puneet
P
Division of BioMedical Sciences, Faculty of Medicine, Memorial University, St. John's, NL, Canada.
Tabrizchi
Reza
R
D000818:Animals; D017609:Barium Compounds / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000502:physiology; D009419:Nerve Tissue Proteins; D010042:Ouabain / Q000494:pharmacology; D024683:Potassium Channels, Tandem Pore Domain / Q000378:metabolism; D011651:Pulmonary Artery / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
D017609:Barium Compounds; D002712:Chlorides; D009419:Nerve Tissue Proteins; D024683:Potassium Channels, Tandem Pore Domain; D011943:Receptors, Adrenergic, beta; C024986:barium chloride; C420132:potassium channel subfamily K member 3; D010042:Ouabain; D019789:Tetraethylammonium; D000254:Sodium-Potassium-Exchanging ATPase; D007545:Isoproterenol