title: Quorum sensing in Aeromonas species isolated from patients in Malaysia.
abstract: Bacterial quorum sensing signal molecules called N-acylhomoserine lactone (AHL) controls the expression of virulence determinants in many Gram-negative bacteria. We determined AHL production in 22 Aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). All isolates produced the two principal AHLs, N-butanoylhomoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). Ten isolates also produced additional AHLs. This report is the first documentation of Aeromonas sobria producing C6-HSL and two additional AHLs with N-acyl side chain longer than C(6). Our data provides a better understanding of the mechanism(s) of this environmental bacterium emerging as a human pathogen.
authors.affiliation: Institute of Biological Sciences (Division of Genetics and Molecular Biology), Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. kokgan@um.edu.my
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
authors.forename: Savithri D
authors.initials: SD
authors.lastname: Puthucheary
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Chan
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.forename: Cheng-Siang
authors.initials: CS
authors.lastname: Wong
authors.forename: Wah-Seng See
authors.initials: WS
authors.lastname: Too
authors.forename: Kek-Heng
authors.initials: KH
authors.lastname: Chua
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D000369:Aged, 80 and over; D002675:Child, Preschool; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D008296:Malaysia; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D053038:Quorum Sensing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S