Modifications of band 3 and oxidation level of membrane proteins in senescent erythrocytes.
OBJECTIVE
To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN ANDMETHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups.


RESULTS
Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC.


CONCLUSIONS
These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.
Rucci
Angel
A
Facultad de Ciencias Bioquímicas y Farmacéuticas. Universidad Nacional de Rosario, Argentina.
Ensinck
María Alejandra
MA
Mufarrege
Nicolas
N
Cotorruelo
Carlos
C
Borrás
Silvia García
SG
Racca
Liliana
L
Biondi
Claudia
C
Racca
Amelia
A
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins