Leptin protects cardiomyocytes from serum-deprivation-induced apoptosis by increasing anti-oxidant defence.
1. Leptin, an important adipose-derived hormone, can be associated with cardiac pathophysiology; however, the role of leptin in cardiomyocyte apoptosis is poorly understood. The present study examines serum-deprivation-induced apoptosis in primary cultured cardiomyocytes treated with leptin. 2. Cardiomyocytes were subjected to serum deprivation in the presence or absence of leptin (5 or 50 nmol/L) for 48 h. Apoptosis was determined by Hoechst 33258 and Annexin V-FITC/propidium iodide dual staining. Cell viability, malondialdehyde (MDA) content, caspase 3 activation, and the expression and enzyme activity of superoxide dismutase (SOD) were measured. Small interference RNA (siRNA) targeting SOD1 and SOD2 were used to knockdown their expression and measure apoptosis. 3. Serum deprivation caused nearly 30% of apoptosis in cardiomyocytes, and an approximately 60% decrease in cell viability. The mRNA levels and the activated form of caspase 3 were greatly increased. In the presence of leptin, the apoptotic rate was reduced to approximately 15%, cell viability was increased and the activation of caspase 3 was partially inhibited. Additionally, the augmented lipid peroxidation (MDA formation) was abolished, and the impaired activities of SOD1 and SOD2 were restored by leptin. The mRNA expression of SOD2, but not SOD1, was stimulated by leptin. Transfection with siRNA that cause deficiency of either SOD1 or SOD2 attenuated the anti-apoptotic effects of leptin. 4. The results suggest that leptin inhibits serum-deprivation-induced apoptosis in cardiomyocytes by activating SOD. The present study outlines the direct actions of leptin in cardiac disorders that are related to elevated leptin levels.
Zheng
Juan
J
Departments of Physiology, Second Military Medical University, Shanghai, China.
Fang
Ji
J
Yin
Yuan-Jun
YJ
Wang
Xiao-Chen
XC
Ren
An-Jing
AJ
Bai
Jie
J
Sun
Xue-Jun
XJ
Yuan
Wen-Jun
WJ
Lin
Li
L
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3 / Q000096:biosynthesis; D061945:Caspase Inhibitors; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D020738:Leptin / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D061945:Caspase Inhibitors; D016895:Culture Media, Serum-Free; D020738:Leptin; C505410:Casp3 protein, rat; D053148:Caspase 3