[Effect of Dureping injection on TIR signal pathway on Ana-1 cells].
OBJECTIVE
To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.


METHODS
Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR.


RESULTS
Dureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly.


CONCLUSIONS
Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.
Zhang
Yanli
Y
Department of Etiology and Immunology of Ningxia Medical University, Yinchuan 750004, China.
Wang
Ningping
N
Gu
Ligang
L
Li
Pengtao
P
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002477:Cells; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D048029:TNF Receptor-Associated Factor 6 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6; D051996:Transcription Factor RelA; C507773:IRAK4 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases