[Identification of a Vibrio splendidus strain isolated from ill Sciaenops ocellatus].
OBJECTIVE
We isolated bacterial strain named M-1 from ill Sciaenops ocellatus with ulceration, and further analyzed the phylogenetics of the bacterial strain.


METHODS
We characterized the strain on the basis of its morphology, physiology, and sequence comparison of 16SrRNA and HSP60 genes.


RESULTS
Strain M-1 was identified as Vibrio splendidus. 16SrRNA gene of strain M-1 and Vibrio splendidus (AJ874361) had nearest kinship, and the analysis of HSP60 gene sequence indicated that strain M-1 and Vibrio (EU653883, AY837440) located in the same phylogenetic tree.


CONCLUSIONS
The results suggested that strain M-1 was a member of Vibrio splendidus.
Ma
Aimin
A
Zhejiang Mariculture Research Institute, Wenzhou 325005, China. ammaaimin@163.com
Yan
Maocang
M
Hu
Lihua
L
Xie
Qilang
Q
Chen
Shaobo
S
Shan
Lezhou
L
Shao
Xinbin
X
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis*; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014733:Vibrio / Q000235:genetics / Q000302:isolation & purification*; D014735:Vibrio Infections / Q000382:microbiology*
D004269:DNA, Bacterial