Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.
Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
Christoffersen
S
S
Department of Cell and Organism Biology, Lund University, Lund, Sweden.
Serra
I
I
Terreni
M
M
Piskur
J
J
D002851:Chromatography, High Pressure Liquid; D003016:Clostridium perfringens / Q000201:enzymology*; D016049:Didanosine / Q000138:chemical synthesis / Q000378:metabolism*; D010430:Pentosyltransferases / Q000378:metabolism*
D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D016049:Didanosine