title: A NH2 tau fragment targets neuronal mitochondria at AD synapses: possible implications for neurodegeneration.
abstract: Synapses are ultrastructural sites for memory storage in brain, and synaptic damage is the best pathologic correlate of cognitive decline in Alzheimer's disease (AD). Post-translational hyperphosphorylation, enzyme-mediated truncation, conformational modifications, and aggregation of tau protein into neurofibrillary tangles (NFTs) are hallmarks for a heterogeneous group of neurodegenerative disorders, so-called tauopathies. AD is a secondary tauopathy since it is pathologically distinguished by the presence of amyloid-beta (Abeta)-containing senile plaques and the presence of tau-positive NFTs in the neocortex and hippocampus. Here, we report that a 20-22 kDa NH2-truncated tau fragment is largely enriched in human mitochondria from cryopreserved synaptosomes of AD brains and that its amount in terminal fields correlates with the pathological synaptic changes and with the organelle functional impairment. This NH2-truncated tau form is also found in other human, not AD-tauopathies, while its presence in AD patients is linked to Abeta multimeric species and likely to pathology severity. Finally native, patient-derived, Abeta oligomers-enriched extracts likely impair the mitochondrial function by the in vitro production of 20-22 kDa NH2-tau fragments in mature human SY5Y and in rat hippocampal neurons. Thus our findings suggest that the mitochondrial NH2-derived tau peptide distribution may exacerbate the synapse degeneration occurring in tauopathies, including AD, and sustain the in vivo NH-2 tau cleavage inhibitors as an alternative drug discovery strategies for AD therapy.
authors.affiliation: Institute of Neurobiology and Molecular Medicine-CNR, IRCSS Fondazione Santa Lucia, Rome, Italy. g.amadoro@inmm.cnr.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Amadoro
authors.forename: Veronica
authors.initials: V
authors.lastname: Corsetti
authors.forename: Annarita
authors.initials: A
authors.lastname: Stringaro
authors.forename: Marisa
authors.initials: M
authors.lastname: Colone
authors.forename: Simona
authors.initials: S
authors.lastname: D'Aguanno
authors.forename: Giovanni
authors.initials: G
authors.lastname: Meli
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Ciotti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sancesario
authors.forename: Antonino
authors.initials: A
authors.lastname: Cattaneo
authors.forename: Rossana
authors.initials: R
authors.lastname: Bussani
authors.forename: Delio
authors.initials: D
authors.lastname: Mercanti
authors.forename: Pietro
authors.initials: P
authors.lastname: Calissano
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D009447:Neuroblastoma; D016874:Neurofibrillary Tangles / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013569:Synapses / Q000378:metabolism* / Q000473:pathology; D016875:tau Proteins / Q000378:metabolism*
chemical_list: C054369:MAPT protein, human; C489131:Mapt protein, rat; D010446:Peptide Fragments; D016875:tau Proteins