title: [Simultaneous determination of five active constitutents in Xiaochaihu Tang by HPLC].
abstract: OBJECTIVE
  To establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.
  
  
  METHODS
  A Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.
  
  
  RESULTS
  The accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.
  
  
  CONCLUSIONS
  The method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.
authors.affiliation: Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Liu
authors.forename: Junning
authors.initials: J
authors.lastname: Zhao
authors.forename: Liangchun
authors.initials: L
authors.lastname: Yan
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yi
authors.forename: Jun
authors.initials: J
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D019695:Glycyrrhizic Acid / Q000032:analysis; D007202:Indicators and Reagents / Q000032:analysis; D008516:Medicine, Chinese Traditional; D032267:Rehmannia / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013678:Technology, Pharmaceutical
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D007202:Indicators and Reagents; C461320:shosaiko-to; C038044:baicalin; C006680:baicalein; D019695:Glycyrrhizic Acid; C473995:wogonoside; C085514:wogonin