title: Agaricus bisporus as a source of tyrosinase for phenol detection for future biosensor development.
abstract: Phenols are toxic compounds that are present in several industrial wastewaters, so their quantification has great environmental importance. In order to permit an analytical methodology for in situ monitoring, this work aims to study the application of Agaricus bisporus tissue as a source of tyrosinase and the optimum reaction conditions for the development of a phenol biosensor. Such an enzyme is a polyphenol oxidase that transforms many different phenolic compounds into quinones. Experiments with fungi tissue were performed to evaluate different sizes of tissue (0.5, 1.0 and 1.5 cm), different temperatures (23.5 degrees C to 60 degrees C), and different pH values (6, 7 and 8) to quantify analytically phenol content. Amongst the tested conditions, those that had presented larger efficiency in phenol oxidation were attained with the fungal tissue size of 1 cm, at pH 8.0, in the temperature range from 35 degrees C to 45 degrees C.
authors.affiliation: Department of Biochemical Engineering, Federal University of Rio de Janeiro--UFRJ, School of Chemistry, Rio de Janeiro, Brazil.
authors.forename: L M C
authors.initials: LM
authors.lastname: Silva
authors.forename: A M
authors.initials: AM
authors.lastname: Salgado
authors.forename: M A Z
authors.initials: MA
authors.lastname: Coelho
mesh_terms: D000364:Agaricus / Q000145:classification* / Q000201:enzymology*; D015374:Biosensing Techniques / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000378:metabolism*; D019800:Phenol / Q000032:analysis* / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D019800:Phenol; D014442:Monophenol Monooxygenase