title: Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering.
abstract: Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production.
authors.affiliation: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Higashi 1-1-1, AIST Tsukuba Central 6, Tsukuba 305-8566, Ibaraki, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Marui
authors.forename: Sumiko
authors.initials: S
authors.lastname: Ohashi-Kunihiro
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ando
authors.forename: Marie
authors.initials: M
authors.lastname: Nishimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Koike
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D005818:Genetic Engineering; D010406:Penicillins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D010406:Penicillins; D011994:Recombinant Proteins