title: TCR-mediated ThPOK induction promotes development of mature (CD24-) gammadelta thymocytes.
abstract: T lymphocytes develop into two major lineages characterized by expression of the alphabeta and gammadelta T cell receptor (TCR) heterodimers. Within each major lineage, further specialization occurs, resulting in distinct subsets that differ in TCR specificity, phenotype and functional attributes. Thus, in the murine thymus, two distinct subsets of mature (CD24-) gammadelta cells have been identified, that is NK1.1+ cells, which are enriched for Vgamma1.1 usage and selectively produce IFNgamma on stimulation, and CCR6+ cells, which are enriched for Vgamma2 usage produce IL17. The upstream signals and transcriptional pathways that promote development of these distinct gammadelta subsets remain relatively poorly understood. Here, we show that the Zn-finger transcription factor ThPOK has a critical function in the development of gammadelta thymocytes. Thus, lack of functional ThPOK causes a marked reduction in the percentage and absolute number of mature gammadelta thymocytes, and a particularly severe reduction of NK1.1+ cells. Conversely, constitutive ThPOK expression leads to a striking increase in mature NK1.1+ gammadelta thymocytes. Further, we show that ThPOK induction in gammadelta thymocytes is induced by strong TCR signals mediated by engagement with antibody or high-affinity endogenous ligands, and that an important ThPOK cis-acting element, the distal regulatory element (DRE), is sufficient for this TCR-dependent induction. These results show that ThPOK expression in gammadelta thymocytes is regulated in part by the strength of TCR signalling, identify ThPOK as an important mediator of gammadelta T cell development/maturation, and lend strong support to the view that development of a significant fraction of gammadelta T cells depends on TCR engagement/signalling.
authors.affiliation: Blood Cell Development and Cancer Keystone Program, Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, PA, USA.
authors.forename: Kyewon
authors.initials: K
authors.lastname: Park
authors.forename: Xi
authors.initials: X
authors.lastname: He
authors.forename: Hyung-Ok
authors.initials: HO
authors.lastname: Lee
authors.forename: Xiang
authors.initials: X
authors.lastname: Hua
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: David
authors.initials: D
authors.lastname: Wiest
authors.forename: Dietmar J
authors.initials: DJ
authors.lastname: Kappes
mesh_terms: D000818:Animals; D051927:CD24 Antigen / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*
chemical_list: D051927:CD24 Antigen; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C497890:Cd24a protein, mouse; C497211:Zbtb7b protein, mouse