title: Gastroenteric virus detection in fecal samples from women in Goiânia, State of Goiás, Brazil.
abstract: BACKGROUND
  This was a prospective study that included women seen in the obstetrics and gynecology sector of Hospital das Clínicas, Federal University of Goiás, in Goiânia, State of Goiás, with the aim of detecting rotaviruses, adenoviruses, caliciviruses and astroviruses. Eighty-four women participated in the study and from these, 314 fecal samples were collected. Out of all of the women, 29 were seropositive for HIV and 55 were seronegative, and 45 and 39 were pregnant and non-pregnant, respectively.
  
  
  METHODS
  Fecal samples were collected from each woman once every two months over the period from July 2006 to June 2007, and they were screened for rotaviruses by means of polyacrylamide gel electrophoresis and immunoenzymatic assays, for caliciviruses and astroviruses by means of RT-PCR and for adenovirus by means of immunoenzymatic assays. The astroviruses were genotyped using nested PCR.
  
  
  RESULTS
  Among the 84 patients, 19 (22.6%) were positive for either calicivirus (14/19) or astrovirus (6/19), while one women was positive for both viruses in fecal samples collected on different occasions. Most of the positive samples were collected during the months of July and August (astrovirus) and September and October (calicivirus). None of the samples analyzed was positive for rotavirus or adenovirus. Gastroenteric viruses were detected in 13/19 (68.4%) of the pregnant women, whether HIV-seropositive or not.
  
  
  CONCLUSIONS
  The results from the present study showed that neither pregnancy nor HIV-seropositive status among the women increased the risk of infection by any of the gastroenteric viruses studied. This study presents data on gastroenteric virus detection among pregnant and/or HIV-positive women.
authors.affiliation: Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, GO, Brazil. ruigilberto@fertile.com.br
authors.forename: Rui Gilberto
authors.initials: RG
authors.lastname: Ferreira
authors.forename: Ana Maria Tavares
authors.initials: AM
authors.lastname: Borges
authors.forename: Fabiola Souza
authors.initials: FS
authors.lastname: Fiaccadori
authors.forename: Menira Borges de Lima Dias E
authors.initials: MB
authors.lastname: Souza
authors.forename: Rodrigo Alessandro Togo
authors.initials: RA
authors.lastname: Santos
authors.forename: Divina das Dôres de Paula
authors.initials: Dd
authors.lastname: Cardoso
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000256:Adenoviridae / Q000302:isolation & purification*; D000328:Adult; D001938:Brazil; D002139:Caliciviridae / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D001257:Mamastrovirus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D015995:Prevalence; D011446:Prospective Studies; D012328:RNA Viruses / Q000145:classification / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification; D055815:Young Adult