title: Recombination analysis reveals a double recombination event in hepatitis E virus.
abstract: Recombination of Hepatitis E Virus (HEV) has rarely been reported. In the present study, phylogenetic and recombination analyses were performed on 134 complete HEV genomes. Three potentially significant recombination events, including both intra-genotype and one inter-genotype, were identified by recombination detection analysis. Recombination events I and II occurred intra-genotype and inter-genotype, respectively, among three isolates, including the lineage represented by CHN-XJ-SW13 (GU119961, swine isolate), E067-SIJ05C (AB369690, human isolate), and JJT-Kan (AB091394, human isolate), and lead to the recombinant swine isolate swCH31 (DQ450072). Recombination event III occurred between the lineage represented by the NA1 (M73218) and K52-87 (L25595), which resulted in the recombinant Xingjiang-1 (D11092). Our analyses proved that that recombination could occur between human and swine HEV strains, double recombination events existed in HEV, and recombination event could happen within ORF2 region of HEV. These results will provide valuable hints for future research on HEV diversity.
authors.affiliation: School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Ni
authors.forename: Hongxing
authors.initials: H
authors.lastname: Shen
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Song
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.forename: Shihe
authors.initials: S
authors.lastname: Shao
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
authors.forename: Li
authors.initials: L
authors.lastname: Cui
mesh_terms: D000818:Animals; D016679:Genome, Viral; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D013552:Swine; D013553:Swine Diseases / Q000821:virology*