A mouse knockout library for secreted and transmembrane proteins.
Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the cardiovascular system. The majority of knockout lines exhibited altered phenotypes in at least one of these therapeutic areas. To our knowledge, a comprehensive phenotypic assessment of a large number of mouse mutants generated by a gene-specific approach has not been described previously.
Tang
Tracy
T
Department of Molecular Biology, Genentech, Inc., South San Francisco, California, USA.
Li
Li
L
Tang
Jerry
J
Li
Yun
Y
Lin
Wei Yu
WY
Martin
Flavius
F
Grant
Deanna
D
Solloway
Mark
M
Parker
Leon
L
Ye
Weilan
W
Forrest
William
W
Ghilardi
Nico
N
Oravecz
Tamas
T
Platt
Kenneth A
KA
Rice
Dennis S
DS
Hansen
Gwenn M
GM
Abuin
Alejandro
A
Eberhart
Derek E
DE
Godowski
Paul
P
Holt
Kathleen H
KH
Peterson
Andrew
A
Zambrowicz
Brian P
BP
de Sauvage
Frederic J
FJ
D000818:Animals; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout
D008565:Membrane Proteins