title: Calcitonin gene-related peptide, substance P, nitric oxide and epinephrine modulate bone marrow micro circulation of the rabbit tibia and femur.
abstract: Different bones have different blood supplies, which may influence bone healing. Therefore, elucidation of the mechanisms involved in the regulation of bone marrow blood flow in different bones is of high clinical importance. To assess the micro circulation of bone marrow of the femur and tibia simultaneously, flow velocities were continuously measured by a two-channel laser-Doppler flowmeter. The probes were introduced into the femoral and tibial diaphysis, respectively, in the anesthetized rabbit. Changes in micro circulation of the bone marrow were elicited by intra-arterial bolus injections of vasoactive substances: epinephrine (E), calcitonine-gene related peptide (CGRP), substance P (SP), sodium nitroprusside (SNP), E and Ebrantil. Systemic arterial blood pressure was recorded with an electro-manometer. Micro vascular resistance (MVR) and 50% recovery time (50RT) to baseline flow level were calculated from the measured data. Flow velocity in the femur was significantly higher. Epinephrine considerably reduced micro vascular blood flow, which could be significantly warded off by Ebrantil. CGRP and SP did not change MVR. Application of SNP resulted in reduction of flow velocity, but it also decreased MVR. No statistically significant differences were found between reactions of the micro circulation in the two marrows. These results suggest that there are no significant differences between the blood flow response patterns of these two bone marrow sites, thus the regulation patterns of the micro circulation of the two bones are also similar.
authors.affiliation: National Institute of Traumatology and Emergency Medicine, Department of Experimental Surgery, Budapest, Hungary. vendegh@vivamail.hu
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Vendégh
authors.forename: András
authors.initials: A
authors.lastname: Melly
authors.forename: Balázs
authors.initials: B
authors.lastname: Tóth
authors.forename: Konrad
authors.initials: K
authors.lastname: Wolf
authors.forename: Tamás
authors.initials: T
authors.lastname: Farkas
authors.forename: István
authors.initials: I
authors.lastname: Kádas
authors.forename: János
authors.initials: J
authors.lastname: Hamar
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000098:blood supply*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology*; D005269:Femur / Q000098:blood supply; D008833:Microcirculation / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects; D013373:Substance P / Q000494:pharmacology*; D013977:Tibia / Q000098:blood supply*; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D009599:Nitroprusside; D009569:Nitric Oxide; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D004837:Epinephrine