Antiinflammatory effects of Epimedium brevicornum water extract on lipopolysaccharide-activated RAW264.7 macrophages.
Epimedium brevicornum Maxim (Berberidaceae) possesses estrogenic properties. It is one of the most widespread herbal remedies used in Oriental medicine. The present study investigated the effects of Epimedium brevicornum water extract (EB) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced RAW264.7 macrophages. EB significantly inhibited the production of nitric oxide (NO), interleukin (IL)-3, IL-10, IL-12p40, interferon-inducible protein-10, keratinocyte-derived chemokine, vascular endothelial growth factor, monocyte chemotactic protein-1 and granulocyte macrophage-colony stimulating factor in LPS-induced RAW264.7 cells at concentrations of 25, 50, 100 and 200 μg/mL (p < 0.05). These results suggest that EB has antiinflammatory activity related to its inhibition of NO, cytokine, chemokine and growth factor production in macrophages.
Yuk
Sang-Suk
SS
College of Oriental Medicine, Kyungwon University, Seongnam, Republic of Korea.
Lim
Eun-Mee
EM
Lee
Ji Young
JY
Lee
Young-Jong
YJ
Kim
Yoon-Sang
YS
Lee
Tae Hee
TH
Park
Seong Kyu
SK
Bae
Hyunsu
H
Kim
Hyung Min
HM
Ko
Seong-Gyu
SG
Oh
Myung Sook
MS
Park
Wansu
W
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D034181:Epimedium / Q000737:chemistry*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide