title: Curdlan-mediated regulation of human phagocyte-specific chitotriosidase.
abstract: Human phagocyte-specific chitotriosidase is part of innate immunity and shows anti-fungal activity towards chitin-containing fungi. We investigated the effect of stimulation of the C-type lectin receptor dectin-1 by beta-1,3-glucan (curdlan) on chitotriosidase expression and release by human phagocytes. We observed that curdlan triggers chitotriosidase release from human neutrophils. In addition, we show that curdlan impairs chitotriosidase induction in monocytes. Finally, curdlan temporarily induces chitotriosidase in enzyme-expressing monocyte-derived macrophages, followed by reduction of chitotriosidase expression after prolonged stimulation. These data on regulation of phagocyte-specific chitotriosidase following curdlan recognition support an important role of chitotriosidase in the elimination of chitin-containing pathogens.
authors.affiliation: Department of Medical Biochemistry, University of Amsterdam, Academic Medical Center, Amsterdam, The Netherlands. m.c.vaneijk@amc.uva.nl
authors.forename: Marco
authors.initials: M
authors.lastname: van Eijk
authors.forename: Tineke
authors.initials: T
authors.lastname: Voorn-Brouwer
authors.forename: Saskia S
authors.initials: SS
authors.lastname: Scheij
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Verhoeven
authors.forename: Rolf G
authors.initials: RG
authors.lastname: Boot
authors.forename: Johannes M F G
authors.initials: JM
authors.lastname: Aerts
mesh_terms: D002454:Cell Differentiation / Q000276:immunology; D002686:Chitin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D006596:Hexosaminidases; D006801:Humans; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism; D047071:beta-Glucans; D000099181:Dectin-1
chemical_list: D002686:Chitin; D006596:Hexosaminidases; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D047071:beta-Glucans; D000099181:Dectin-1; C038459:curdlan; C033363:beta-1,3-glucan; C086400:chitotriosidase