title: Effects of homogeneous and inhomogeneous static magnetic fields combined with gamma radiation on DNA and DNA repair.
abstract: The aim of this study was to reveal whether static magnetic fields (SMFs) influence the repair of radiation-damaged DNA on leukocytes or has any effect on DNA. After 4 Gy of (60)Co-gamma irradiation, some of the samples were exposed to inhomogeneous SMFs with a lateral magnetic flux density gradient of 47.7, 1.2, or 0.3 T/m by 10 mm lateral periodicity, while other samples were exposed to homogeneous SMF of 159.2 +/- 13.4 mT magnetic flux density for a time period of 0.5 min, 1, 2, 4, 6, 18, 20, or 24 h. Another set of samples was exposed to the aforementioned SMFs before gamma irradiation. The following three groups were examined: (i) exposed to SMF only, (ii) exposed to SMF following irradiation by (60)Co-gamma, and (iii) exposed to SMF before (60)Co-gamma irradiation. The analysis of the DNA damage was made by single-cell gel electrophoresis technique (comet assay). Statistically significant differences were found at 1 h (iSMF), 4 h (hSMF), and 18 h (hSMF) if samples were exposed to only SMF, compared to control. When the SMF exposure followed the (60)Co-gamma irradiation, statistically significant differences were found at 1 h (iSMF) and 4 h (hSMF). If exposure to SMF preceded (60)Co-gamma irradiation, no statistically significant difference was found compared to 4 Gy gamma-irradiated group.
authors.affiliation: "Frédéric Joliot-Curie" National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary.
authors.forename: Györgyi
authors.initials: G
authors.lastname: Kubinyi
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Zeitler
authors.forename: György
authors.initials: G
authors.lastname: Thuróczy
authors.forename: Péter
authors.initials: P
authors.lastname: Juhász
authors.forename: József
authors.initials: J
authors.lastname: Bakos
authors.forename: Hanna
authors.initials: H
authors.lastname: Sinay
authors.forename: János
authors.initials: J
authors.lastname: László
mesh_terms: D000328:Adult; D004247:DNA / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000528:radiation effects; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D004247:DNA