Proteomic analysis of protein expression affected by peroxiredoxin V knock-down in hypoxic kidney.
Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and the nucleus. Prdx V has been reported to protect a wide range of cellular environments as an antioxidant enzyme, and its dysfunctions may be implicated in several diseases, such as cancer, inflammation, and neurodegenerative disease. Identification and relative quantification of proteins affected by Prdx V may help identify novel signaling mechanisms that are important for oxidative stress response. However, the role of Prdx V in the modulation of hypoxia-related cellular response is not studied yet. To examine the function of endogenous Prdx V in hypoxic condition in vivo, we generated a transgenic mouse model with Prdx V siRNA expression controlled by U6 promoter. Of many tissues, the knockdown of Prdx V expression was displayed in the kidney, lung, and liver but not the spleen and skin. We conducted on the basis of nano-UPLC-MS(E) proteomic study to identify the Prdx V-affected protein networks in hypoxic kidneys. In this study, we identified protein networks associated with oxidative stress, fatty acid metabolism, and mitochondrial dysfunction. Our results indicated that Prdx V affected to regulation of kidney homeostasis under hypoxia stress.
Yang
Hee-Young
HY
Department of Oral Biochemistry, Dental Science Research Institute, The second Stage of Brain Korea 21 for Dental School, Chonnam National University, Gwangju, Republic of Korea.
Kwon
Joseph
J
Cho
Eun-Jin
EJ
Choi
Hoon-In
HI
Park
Chiyoul
C
Park
Hyang-Rim
HR
Park
Sung-Hee
SH
Chung
Kyoung-Jin
KJ
Ryoo
Zae Young
ZY
Cho
Kyoung-Oh
KO
Lee
Tae-Hoon
TH
D000818:Animals; D015153:Blotting, Western; D015687:Cell Hypoxia / Q000502:physiology*; D002853:Chromatography, Liquid; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D055785:Gene Knockdown Techniques; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000502:physiology*; D054464:Peroxiredoxins / Q000172:deficiency / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology*; D053719:Tandem Mass Spectrometry
D017931:DNA Primers; D054464:Peroxiredoxins