Could protein tertiary structure influence mammary transgene expression more than tissue specific codon usage?
Animal mammary glands have been successfully employed to produce therapeutic recombinant human proteins. However, considerable variation in animal mammary transgene expression efficiency has been reported. We now consider whether aspects of codon usage and/or protein tertiary structure underlie this variation in mammary transgene expression.
He
Zuyong
Z
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, 510006, Guangzhou, People's Republic of China.
Zhao
Yiqiang
Y
Mei
Gui
G
Li
Ning
N
Chen
Yaosheng
Y
D000818:Animals; D030801:Animals, Genetically Modified; D003062:Codon / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012756:Sheep; D013552:Swine; D014018:Tissue Distribution; D019076:Transgenes / Q000235:genetics / Q000502:physiology
D003062:Codon; D011994:Recombinant Proteins