[Preliminary evaluation of immunological function in patients with acute lymphocytic leukemia].
This study was aimed to investigate 6 kinds of human cytokines (IL-2, IL-4, IL-12, IL-13, IFN-gamma and TNF-alpha) in patients with acute lymphocytic leukemia (ALL), so as to find their relationship with the disease. The pure monoclonal antibodies were spotted on the prepared NC membrane glass slides under certain conditions to make human cytokine protein microarray. The serum samples were collected from peripheral blood of 28 patients and 25 normal controls, the serum concentration of cytokines was determined by using the protein microarray and ELISA. The results showed that the expression levels of IL-4, IL-12, IL-13, IFN-gamma and TNF-alpha in ALL patients were lower than those in the normal controls, but ELISA indicated that the expression of TNF-alpha in ALL patients and normal controls was negative. In conclusion, both cellular and humoral immunity are all inhibited in patients with ALL. Microarray is more sensitive than ELISA.
Wu
Ying-Ya
YY
Department of Hematology, Guiyang Medical College, Guigyang, Guizhon Province, China.
Wang
Ji-Shi
JS
Fang
Qing
Q
D000328:Adult; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D018793:Interleukin-13 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000276:immunology*; D040081:Protein Array Analysis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D018793:Interleukin-13; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma