title: Suspended nanoparticle crystal (S-NPC): A nanofluidics-based, electrical read-out biosensor.
abstract: Here we report an approach utilizing a suspended nanoparticle crystal (S-NPC) as an electrical read-out biosensor based on a nanofluidic electrokinetics principle. As a preliminary demonstration, streptavidin-modified S-NPC with a particle diameter of 520 nm was used to detect biotin in a PBS buffer. The present result indicated that the detection range of biotin by this nanofluidics-based biosensor was about 1 nM-10 muM (in 10(-4)x PBS) with a sensitivity of 160 nS/nM. Being easy to get established, low-cost, and having large electrical read-out signal, the present S-NPC is believed to be a promising biosensing scheme in the micro total analysis system.
authors.affiliation: Institute of Microelectronics, Peking University, Beijing, PR China.
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Lei
authors.forename: Fei
authors.initials: F
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wengang
authors.initials: W
authors.lastname: Wu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D004560:Electricity; D044085:Microfluidics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013535:Suspensions
chemical_list: D013535:Suspensions; D001710:Biotin; D019809:Streptavidin