Taenia saginata: production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens.
Cysticercosis is a major cause of economic loss in bovine production due to meat condemnation. Chemotherapy is being used in Brazilian cattle and a diagnostic test to improve the treatment program is desired. We produced monoclonal antibodies against crude (TAEB) and cyst fluid (TAEF) Taenia saginata metacestode antigens using immunized BALB/c mice. After cell fusion, 10 TAEB and nine TAEF hybrids were selected and cloned resulting in 18 IgG(1) and 32 IgM TAEB clones, and 9 IgG(1) and 9 IgM TAEF clones. Ascites was produced and Western blot testing was performed resulting in reactivity to protein fractions of low molecular weight (<18kDa), 43, 55, 66 and 100kDa. The indirect immunofluorescence test, with one monoclonal antibody against crude and one against cyst fluid antigens, recognized antigenic fractions of both the scolex and the bladder wall of metacestodes from naturally infected bovine.
Vicentini-Oliveira
Josy Campanhã
JC
UNESP, Univ. Estadual Paulista, Campus Botucatu, Graduation Program on Veterinary Medicine, School of Veterinary Medicine and Zootechny, Botucatu, SP, Brazil.
Golim
Marjorie A
MA
de Cássia Paulan
Silvana
S
Biondi
Germano Francisco
GF
Rossi-Ferreira
Rosana
R
Deffune
Elenice
E
Nunes
Cáris Maroni
CM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D041202:Taenia saginata / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth