[Determination of arsanilic acid and sulfanilic acid as adulterant in feed additives by reversed-phase high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic (RP-HPLC) method was established for the determination of arsanilic acid and sulfanilic acid as adulterant in the feed additives. The separation was carried out on a Waters Bondapak C18 column, and methanol-water (pH 2.9 adjusted by 0.01 mol/L phosphoric acid) (1 : 4, v/v) was used as the mobile phase with a flow rate of 1.0 mL/min. A diode array detector was used at 244 nm as the detection wavelength. Arsanilic acid and sulfanilic acid were separated within 3 min. The linear ranges all were 5 - 200 mg/L and the detection limits (S/N = 3) were 0.20 and 0.15 mg/L for arsanilic acid and sulfanilic acid, respectively. This method is simple and rapid, and suitable for the simultaneous determination of arsanilic acid and sulfanilic acid in feed additives.
Xu
Jinping
J
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. jp1129@tom.com
He
Heng
H
Xu
Mengyi
M
Qu
Yanhua
Y
D000821:Animal Feed / Q000032:analysis*; D001147:Arsanilic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D013425:Sulfanilic Acids / Q000032:analysis*
D005503:Food Additives; D013425:Sulfanilic Acids; D001147:Arsanilic Acid