[Inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro].
To investigate the inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro.



The ability of SG235 to infect leukemia cells and the expression of CD46 on blasts from the patient with leukemia were detected by flow cytometry (FACS). The cytotoxicity of the virus was evaluated by MTT assay. Apoptosis induced by SG235 was detected with Annexin-V/PI staining and TUNEL assay followed by FACS analysis.



The majority of leukemia cells from the patient with acute leukemia was CD46-positive. GFP-positive cells were 45.1%, 35.7%, 54.2%, 37.0%, 30.1%, %67.1, 17.2% and 33.1% in Mutz-1, Kasumi-1, K562, HL60, Molt- 4, RPMI8226, L428, and Jurkat cell lines treated with SG235-EGFP vector at MOI (multiplicity of infection) of 50 for 48 h.SG235 treatment resulted in marked growth inhibition and apoptosis of Kassumi-1 cells, and also significantly inhibited expression of p-Akt.



The chimeric oncolytic adenovirus SG235 can infect leukemia cell effectively and results in the growth inhibition and apoptosis of Kasumi-1 cells in vitro.
Yang
Chun-mei
CM
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Liu
Hui
H
Yang
Xiu-di
XD
Jin
Jie
J
Qian
Wen-bin
WB
D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051916:Membrane Cofactor Protein / Q000378:metabolism; D050504:Oncolytic Viruses; D014162:Transfection
D051916:Membrane Cofactor Protein