Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
In the mouse olfactory system, the anatomical locations of olfactory sensory neurons (OSNs) roughly correlate with their axonal projection sites along the dorsal-ventral (D-V) axis of the olfactory bulb (OB). Here we report that an axon guidance receptor, Neuropilin-2 (Nrp2), and its repulsive ligand, Semaphorin-3F (Sema3F), are expressed by OSNs in a complementary manner that is important for establishing olfactory map topography. Sema3F is secreted by early-arriving axons of OSNs and is deposited at the anterodorsal OB to repel Nrp2-positive axons that arrive later. Sequential arrival of OSN axons as well as the graded and complementary expression of Nrp2 and Sema3F by OSNs help to form the topographic order along the D-V axis.
Takeuchi
Haruki
H
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan.
Inokuchi
Kasumi
K
Aoki
Mari
M
Suto
Fumikazu
F
Tsuboi
Akio
A
Matsuda
Ikuo
I
Suzuki
Misao
M
Aiba
Atsu
A
Serizawa
Shou
S
Yoshihara
Yoshihiro
Y
Fujisawa
Hajime
H
Sakano
Hitoshi
H
D000818:Animals; D001369:Axons / Q000378:metabolism*; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D039943:Neuropilin-2 / Q000378:metabolism; D009830:Olfactory Bulb / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D049951:X Chromosome Inactivation
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D039943:Neuropilin-2; C492112:Plxna3 protein, mouse; D011956:Receptors, Cell Surface; C491229:Sema3f protein, mouse