title: A mouse knockout library for secreted and transmembrane proteins.
abstract: Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the cardiovascular system. The majority of knockout lines exhibited altered phenotypes in at least one of these therapeutic areas. To our knowledge, a comprehensive phenotypic assessment of a large number of mouse mutants generated by a gene-specific approach has not been described previously.
authors.affiliation: Department of Molecular Biology, Genentech, Inc., South San Francisco, California, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jerry
authors.initials: J
authors.lastname: Tang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Wei Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Flavius
authors.initials: F
authors.lastname: Martin
authors.forename: Deanna
authors.initials: D
authors.lastname: Grant
authors.forename: Mark
authors.initials: M
authors.lastname: Solloway
authors.forename: Leon
authors.initials: L
authors.lastname: Parker
authors.forename: Weilan
authors.initials: W
authors.lastname: Ye
authors.forename: William
authors.initials: W
authors.lastname: Forrest
authors.forename: Nico
authors.initials: N
authors.lastname: Ghilardi
authors.forename: Tamas
authors.initials: T
authors.lastname: Oravecz
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Platt
authors.forename: Dennis S
authors.initials: DS
authors.lastname: Rice
authors.forename: Gwenn M
authors.initials: GM
authors.lastname: Hansen
authors.forename: Alejandro
authors.initials: A
authors.lastname: Abuin
authors.forename: Derek E
authors.initials: DE
authors.lastname: Eberhart
authors.forename: Paul
authors.initials: P
authors.lastname: Godowski
authors.forename: Kathleen H
authors.initials: KH
authors.lastname: Holt
authors.forename: Andrew
authors.initials: A
authors.lastname: Peterson
authors.forename: Brian P
authors.initials: BP
authors.lastname: Zambrowicz
authors.forename: Frederic J
authors.initials: FJ
authors.lastname: de Sauvage
mesh_terms: D000818:Animals; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout
chemical_list: D008565:Membrane Proteins