A parallel scheme for comparing transcription factor binding sites matrices.
Gene regulation implies many mechanisms. Their identification is a crucial task to construct regulatory networks, and is necessary to understand the pathology in many cases. This requires the identification of transcription factors that play a role in regulation. Numerous motif discovery tools are now available. Combining efficiently their results appears useful for comparing and clustering these motifs in order to reduce redundancies and to identify the corresponding transcription factor. We develop a method that produces, compares and clusters a set of motifs and identifies some close motifs in databases like JASPAR and the public version of Transfac. Unlike previous comparison methods, where each matrix column is compared independently, we have developed a global method to compare motifs that also helps to reduce the number of false positives. We also propose an original graph motif model that generalizes the classical position specific pattern matrices. Finally, we present an application of our method to study ChIP-chip data sets in the context of an eukaryotic organism.
Carat
Solenne
S
Institut du thorax, INSERM U 915, Université de Nantes, France. Solenne.Carat@univ-nantes.fr
Houlgatte
Rémi
R
Bourdon
Jérémie
J
D000465:Algorithms; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*
D014157:Transcription Factors; D004247:DNA