title: Downregulation of immune response by the human cytokines Interleukin-32alpha and beta in cell-mediated rejection.
abstract: Xenotransplantation of porcine organs has the potential to help overcome the severe shortage of human tissues and organs available for human transplantation. However, numerous hurdles such as immune-mediated xenograft rejection remain before clinical xenotransplantation. In this study, we elucidated the role of human TNF-alpha-inducing factor, Interleukin-32 (IL-32), in porcine kidney cells (PK-15) during cell-mediated rejection by examining host cell responses. CD8+ and CD4+ T cells numbers were reduced in the lymph nodes of PK-15/IL-32beta injected mice. CD3+ Tcells were in mice injected with control cells but PK-15/IL-32alpha- and PK-15/IL-32beta-injected cell numbers were lower in lymph nodes than un transfected controls. In Mixed lymphocyte reaction cultures, the rates of cell proliferation were increased in both PK-15/IL-32alpha- and PK-15/IL-32beta-injected groups compared to the untransfected control groups. The Stable porcine PK-15 cells expression IL-32alpha and IL-32beta inhibited cytotoxic T lymphocyte (CTLs) after cellular xenograft. Our results suggest that human IL-32alpha and IL-32beta regulates on xenograft rejection in cellular xenotransplantation.
authors.affiliation: Department of Oral Pharmacology, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Jung Il
authors.initials: JI
authors.lastname: Chae
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Shim
authors.forename: Kwang Su
authors.initials: KS
authors.lastname: Lee
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Cho
authors.forename: Kyu-Sun
authors.initials: KS
authors.lastname: Lee
authors.forename: Do Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Deog-Bon
authors.initials: DB
authors.lastname: Koo
authors.forename: Chang-Sik
authors.initials: CS
authors.lastname: Park
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.forename: Pyung Keun
authors.initials: PK
authors.lastname: Myung
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D017690:Cell Transplantation; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D007111:Immunity, Cellular; D056747:Immunomodulation; D007378:Interleukins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D013552:Swine; D019076:Transgenes / Q000235:genetics; D014183:Transplantation, Heterologous
chemical_list: C496971:IL32 protein, human; D007378:Interleukins