Expression profiling of cell cycle regulatory proteins in oropharyngeal carcinomas using tissue microarrays.
OBJECTIVE
The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis.


METHODS
Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays.


RESULTS
Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx.


CONCLUSIONS
Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.
Ribeiro
Daniel A
DA
DDS, Departamento de Biociências, Av. Ana Costa, 95, Vila Mathias, Santos - SP, Brazil, 11060-001. daribeiro@unifesp.br
Nascimento
Fabio D
FD
Fracalossi
Ana Carolina C
AC
Gomes
Thiago S
TS
Oshima
Celina T F
CT
Franco
Marcello F
MF
D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009959:Oropharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D011379:Prognosis; D040081:Protein Array Analysis; D016160:Retinoblastoma Protein / Q000378:metabolism; D012720:Severity of Illness Index; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D014408:Biomarkers, Tumor; C493956:CDKN1A protein, human; D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53