title: [Effect of DEHP and its metabolite MEHP on in vitro rat follicular development].
abstract: OBJECTIVE
  To use rat preantral follicle culture as a novel bioassay in order to study the effects of di-2-ethylhexyl phttmlate (DEHP) and its metabolite mono-2-ethylhexyl phthalate (MEHP) on folliculogenesis in vitro.
  
  
  METHODS
  The preantral follicles were mechanically dissected from ovaries and equally randomized to to 96-well plates. Six dose groups were setup to be acute exposed in 2.5, 5, 10, 20, 40 and 80 microg/ml MEHP concentration, 33.7, 67.5, 125, 250, 500 and 1000 nmol/L DEHP concentration on day 2,while another control group used solvent only, each group had 16 to 20 follicles. After subsequently cultured individually for 10 days, the follicles were induced to ovulate for 20h, follicle development and oocyte maturation were observed.
  
  
  RESULTS
  Day 11 follicle survival rate (54.76% +/- 3.37%) had significantly dropped after exposure to >10 microg/ml MEHP concentration, comparing to the control group (91.57% +/- 1.32%), the difference is statistical significant (P < 0.05). There is correlation between dosage and follicle survival rate, with correlation coefficient R2 = 0.92. Abnormal follicle rate (45.24% +/- 3.37%) is remarkably higher, and the difference is statistically significant (P < 0.05) comparing to the control group. At the concentrations of >20 microg/ml MEHP, follicle growth and differentiation were dependently impaired:antral follicles (46.18% +/- 1.67%) had significantly reduced (P < 0.05) comparing to the control group (85.91% +/- 5.03%). DEHP showed no adverse effects upon follicle development, there were no significant defference between DMSO and control.
  
  
  CONCLUSIONS
  At the dose of >10 microg/ml, MEHP could inhibit the in-vitro rat follicle development, DEHP seemed no adverse effects upon follicle development.
authors.affiliation: Department of Health Toxicology, Second Military Medical University, Shanghai 200433, China. wanxuying711@sina.com
authors.forename: Xuying
authors.initials: X
authors.lastname: Wan
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xili
authors.initials: X
authors.lastname: Ma
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Zhu
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Zheng
authors.forename: Juan
authors.initials: J
authors.lastname: Hou
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Tianbao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D010968:Plasticizers / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D010968:Plasticizers; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate