Blocking effect of a monoclonal antibody against recombinant Pvs25 on sporozoite development in Anopheles sinensis.
To develop a vaccine to block the transmission of vivax malaria, the gene encoding the ookinete surface protein Pvs25 was cloned from a Korean malaria patient. The Pvs25 gene was 660 bp long, encoding 219 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, named rPvs25, showed a molecular mass of approximately 25 kDa by SDS-PAGE analysis. An anti-rPvs25 monoclonal antibody produced in BALB/c mice was able to inhibit sporozoite development in the mosquito Anopheles sinensis, which is known as the malaria transmission vector in the Republic of Korea. In addition, rPvs25 produced a relatively high antibody titer in BALB/c mice that lasted for more than 6 months. Based on these results, we suggest that recombinant Pvs25 could be a useful antigen in the development of a vaccine to prevent local malaria transmission in the Republic of Korea.
Moon
Sung-Ung
SU
Department of Pathology, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610, USA.
Kim
Hyung-Hwan
HH
Kim
Tong-Soo
TS
Choi
Kyung-Mi
KM
Oh
Chang-Mi
CM
Ahn
Yong-Joo
YJ
Hwang
Seo-Kyoung
SK
Sohn
Youngjoo
Y
Shin
E-Hyun
EH
Kim
Hyuck
H
Lee
Hyeong-Woo
HW
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004199:Disease Vectors; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017780:Malaria Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016780:Malaria, Vivax / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D034101:Sporozoites / Q000254:growth & development / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017780:Malaria Vaccines; C121473:Pvs25 protein, P vivax; D011994:Recombinant Proteins