Enhanced water-holding capacity of meat was associated with increased Sepw1 gene expression in pigs fed selenium-enriched yeast.
To study the effect of selenium-enriched yeast (SeY) level on selenoprotein genes expression and the relation between gene expression and antioxidant status and meat quality, 30 selenium (Se)-depleted pigs (7-week old, 10.30±0.68 kg) were randomly divided into 3 groups and fed a basal diet plus 0, 0.3 and 3.0 mg Se/kg as SeY for 8 weeks. Results showed that dietary SeY supplementation improved the antioxidant status in muscle. The increased levels of SeY decreased (P<0.05) the drip loss and the concentration of thiobarbituric acid reactive substances in the muscle and meat. However, increased dietary SeY intake quadratically increased (P<0.01) the mRNA level of Sepw1 gene among the 12 selenoprotein genes examined in muscle. Statistical analysis showed drip loss was negatively correlated with the mRNA level of Sepw1 gene. These suggested that the enhanced water-holding capacity of meat was associated with the increased expression of Sepw1 gene.
Li
Jun-Gang
JG
Key Laboratory for Animal Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, 625014, Ya'an, China.
Zhou
Ji-Chang
JC
Zhao
Hua
H
Lei
Xin-Gen
XG
Xia
Xin-Jie
XJ
Gao
Ge
G
Wang
Kang-Ning
KN
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D005524:Food Technology; D015870:Gene Expression / Q000187:drug effects*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D051151:Selenoprotein W / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000378:metabolism; D014867:Water; D015003:Yeasts
D000975:Antioxidants; D012333:RNA, Messenger; D051151:Selenoprotein W; D014867:Water; D012643:Selenium