title: Identification of a linear epitope for Fc-binding in the mouse FcγRIII.
abstract: Fc receptors are transmembrane proteins, found on the surfaces of immune cells, that aid in the removal of foreign pathogens by binding to antibody-coated targets via the Fc regions of the antibodies. To identify sites on mouse FcgammaRIII (moFcgammaRIII) alpha-chain that bind to the Fc region, peptides derived from the proximal extracellular domain (EC2) of moFcgammaRIII alpha-chain corresponding to the homologous region of human FcgammaRIIIB alpha-chain were synthesized. Binding of mouse IgG to the different peptides was tested by Dot-blot assay. The effective peptide (119)SFFHNEKSVRYH(130) located in the putative C-C' loop of the EC2 domain was found to bind mouse IgG specifically with an affinity of approximately 5.58 x 10(-5) M and inhibit the binding of mouse IgG to the receptor. Such a functional peptide should be very useful for further understanding the IgG-FcgammaR interaction and development of FcR-targeting drugs.
authors.affiliation: School of Food Science and Technology, Henan University of Technology, Zhengzhou 450052, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xi
authors.forename: Li N
authors.initials: LN
authors.lastname: Zhang
authors.forename: Guang P
authors.initials: GP
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Song L
authors.initials: SL
authors.lastname: Qiao
authors.forename: Jun Q
authors.initials: JQ
authors.lastname: Guo
authors.forename: Qi Y
authors.initials: QY
authors.lastname: Lu
authors.forename: Gai P
authors.initials: GP
authors.lastname: Zhang
authors.forename: Yan Y
authors.initials: YY
authors.lastname: Yang
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D001665:Binding Sites; D001667:Binding, Competitive; D019556:COS Cells; D002645:Chickens; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000276:immunology; D015151:Immunoblotting; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D017452:Receptors, IgG / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D000939:Epitopes; C485700:Fcgr3 protein, mouse; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009842:Oligopeptides; D010446:Peptide Fragments; D017452:Receptors, IgG; D011994:Recombinant Proteins