Role of host lysosomal associated membrane protein (LAMP) in Trypanosoma cruzi invasion and intracellular development.
Trypanosoma cruzi host cell entry depends on lysosomes for the formation of the parasitophorous vacuole. Lysosome internal surface is covered by two major proteins, highly sialilated, Lysosome Associated Membrane Proteins 1 and 2. T. cruzi, on the other hand, needs to acquire sialic acid from its host cell through the activity of trans-sialidase, an event that contributes to host cell invasion and later for parasite vacuole escape. Using LAMP1/2 knock out cells we were able to show that these two proteins are important for T. cruzi infection of host cells, both in entrance and intracellular development, conceivably by being the major source of sialic acid for T. cruzi.
Albertti
L A G
LA
Department of Morphology, Federal University of Minas Gerais, Antonio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil.
Macedo
A M
AM
Chiari
E
E
Andrews
N W
NW
Andrade
L O
LO
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000469:parasitology; D054884:Host-Pathogen Interactions; D052119:Lysosomal-Associated Membrane Protein 2 / Q000235:genetics / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins / Q000172:deficiency / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D014349:Trypanosoma cruzi / Q000472:pathogenicity*; D052118:Lysosomal-Associated Membrane Protein 1
D052119:Lysosomal-Associated Membrane Protein 2; D051907:Lysosomal Membrane Proteins; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1