title: Observations of the mechanisms of electromediated DNA uptake--from vesicles to tissues.
abstract: We discuss experimental observations of DNA uptake induced by electropermeabilisation. First we describe how experiments on giant unilamelar vesicles can be used to understand the effect of electric fields on lipid membranes and the associated transfer of DNA across the plasma membrane. We then discuss how DNA interacts with electropermeabilised cells in culture and how gene expression is correlated to observations of interactions between DNA and the cell membrane. Finally we discuss how DNA uptake occurs and can be optimized for the most medically relevant case of tissues.
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077 Toulouse, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Golzio
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Escoffre
authors.forename: Thomas
authors.initials: T
authors.lastname: Portet
authors.forename: Chloé
authors.initials: C
authors.lastname: Mauroy
authors.forename: Justin
authors.initials: J
authors.lastname: Teissié
authors.forename: David S
authors.initials: DS
authors.lastname: Dean
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Rols
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D002463:Cell Membrane Permeability; D004247:DNA / Q000378:metabolism*; D004574:Electromagnetic Fields; D018274:Electroporation; D015870:Gene Expression / Q000502:physiology; D018014:Gene Transfer Techniques; D051379:Mice; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: D053835:Unilamellar Liposomes; D004247:DNA