title: Response of Bacillus cereus to gamma-irradiation in combination with carvacrol or mild heat treatment.
abstract: Carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of Bacillus cereus in broth. The bacterium was treated with gamma-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 degrees C. The effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (SEM), the cellular fatty acids composition of the membrane quantified by gas chromatography, the intracellular and extracellular adenosine 5'-triphosphate (ATP) concentrations, and the DNA degradation. Combined treatments resulted in additive or synergistic effects as compared to gamma-irradiation alone. A significant modification (P < or = 0.05) of the fatty acid composition and unsaturation ratios was observed. Pretreatment with mild heat or carvacrol before irradiation disturbed the membrane integrity of B. cereus and induced a significant decrease (P < or = 0.05) of the intracellular ATP concentration. SEM observations revealed that the cell membrane was more severely affected with combined treatment than irradiation alone. The electrophoresis analysis showed that DNA degradation by combined treatments was greater than the gamma-irradiation alone.
authors.affiliation: National Center for Nuclear Sciences and Technologies, Tunis Cedex, Tunisia.
authors.forename: Samia
authors.initials: S
authors.lastname: Ayari
authors.forename: Dominic
authors.initials: D
authors.lastname: Dussault
authors.forename: Mathieu
authors.initials: M
authors.lastname: Millette
authors.forename: Mokhtar
authors.initials: M
authors.lastname: Hamdi
authors.forename: Monique
authors.initials: M
authors.lastname: Lacroix
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001409:Bacillus cereus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D000081007:Cymenes; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005720:Gamma Rays; D006358:Hot Temperature; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D039821:Monoterpenes / Q000494:pharmacology*
chemical_list: D000081007:Cymenes; D004269:DNA, Bacterial; D039821:Monoterpenes; D000255:Adenosine Triphosphate; C073316:carvacrol