Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification.
BACKGROUND
Supercritical CO(2) was utilised to extract Chinese star anise seed oil (CSASO), and a three-level Box-Behnken factorial design from response surface methodology was applied to optimise the extraction conditions, including pressure, temperature and amount of modifier (ethanol). The compositional analysis of fatty acids in CSASO was performed by HPLC with fluorescence detection using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as labelling reagent. Identification was carried out by online atmospheric chemical ionisation-mass spectrometry.


RESULTS
The optimum extraction conditions were as follows: extraction pressure, 27.72 MPa, extraction temperature, 46.22 degrees C, and amount of modifier, 8.58 vol.%. The experimental result showed that the maximum extraction yield was 25.31 +/- 0.22% (w/w) under the conditions proposed. The compositional analysis indicated that CSASO mainly contained C18:2, C18:1, C18:3, C20:4, C16, C18 and C20 fatty acids.


CONCLUSIONS
In this study, a fast, simple and high-efficiency supercritical technique for extracting oil from Chinese star anise seed was developed. Simultaneous determination of fatty acids in CSASO using BCETS as the labelling reagent with HPLC fluorescence detection and online mass spectroscopy identification has been successfully achieved.
Li
Guoliang
G
Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, P.R. China.
Sun
Zhiwei
Z
Xia
Lian
L
Shi
Junyou
J
Liu
Yongjun
Y
Suo
Yourui
Y
You
Jinmao
J
D002245:Carbon Dioxide; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000302:isolation & purification*; D005453:Fluorescence; D029523:Illicium / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry*
D005227:Fatty Acids; D010936:Plant Extracts; D010938:Plant Oils; D002245:Carbon Dioxide