title: Plant-originated glycoprotein (36 kDa) suppresses interleukin-4 and -10 in bisphenol A-stimulated primary cultured mouse lymphocytes.
abstract: Bisphenol A (BPA) is one of the estrogen mimic environmental hormones and a chemical used for the wrapping foods, toy products for children, biomedical equipment, and machines. It can exert toxic effects, such as occurring allergy-related diseases. This study demonstrates that glycoprotein isolated from Rhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines [Interleukin (IL)-4 and -10]. First, it was shown that RVS glycoprotein inhibits the proliferation of lymphocytes and scavenges intracellular reactive oxygen species (ROS). Then, the activities of mitogen-activated protein kinase (MAPK), GATA-binding protein-3 (GATA-3), t-box expressed in T-cells (T-bet), and Th2 cell-related cytokine (IL-4 and -10) were evaluated in BPA (50 microM)-stimulated primary cultured mouse lymphocytes, using immunoblot analysis and reverse-transcription polymerase chain reaction (RT-PCR). The results showed that the RVS glycoprotein (50 microg/mL) inhibited the proliferation of lymphocytes, intracellular ROS, and activity of p38 MAPK dose dependently. In the transcriptional factors for the oriented differentiation of T-helper cells, the RVS glycoprotein (50 microg/mL) significantly suppressed the GATA-3, whereas it enhanced T-bet. Also, the RVS glycoprotein (100 microg/mL) significantly attenuated Th2-related cytokines (IL-4 and -10). Taken together, the results obtained from this study suggest that the RVS glycoprotein may help in preventing allergy-related immune dysfunction, such as that produced by BPA.
authors.affiliation: Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Gwang-ju, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Lee
authors.forename: Kye-Taek
authors.initials: KT
authors.lastname: Lim
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D015151:Immunoblotting; D016753:Interleukin-10 / Q000037:antagonists & inhibitors*; D015847:Interleukin-4 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029045:Rhus / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D006023:Glycoproteins; D016753:Interleukin-10; D015847:Interleukin-4; D010636:Phenols; D017382:Reactive Oxygen Species; C006780:bisphenol A; D000098826:Bisphenol A Compounds