[Genetic diversity of Rehmannia glutinosa germplasms].
OBJECTIVE
To provide molecular evidences for phylogenetic analysis by studying ITS sequences of Rehmannia glutinosa from different areas.


METHODS
The DNAs were extracted from leaves of R. glutinosa by means of CTAB method. The products of PCR amplification were cloned . The data were analyzed by MEGA4.0 software.


RESULTS
The results showed that the size of the ITS of R. glutionsa tested was from 613 to 614 bp and the length variation was only 1 bp. The sequence of ITS1 was 224-225 bp, and G + C content varied from 60.4% to 63%. The sequence of ITS2 was 224-225 bp and G + C content varied from 57.1% to 65.3%. The sequence of 5. 8S rDNA was 164 bp, it's very conservative in these species. Phylogram tree based on ITS sequence data indicated that the kinship between Bejing No. 2 R. glutinosa and the others were far. There was obvious diversity within wild R. glutinosa varieties, while there was no different among cultivated R. glutinosa varieties. In cultivated R. glutinosa varieties, there was no diversity between R. glutinosa varieties from Henan and those from others provinces. In wild varieties, R. glutinosa from Shengnongshan and Qingtianhe of Henan province showed a closer systematic relationship with cultivated R. glutinosa from Shandong province, while there was no difference between wild R. glutinosa varieties and cultivated varieties from Henan and Shanxi provinces.


CONCLUSIONS
The genetic relationship among R. glutinosa varieties was very close, there was no distinct systematic differentiation.
Gao
Suxia
S
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Liu
Hongyan
H
Wang
Fei
F
D018744:DNA, Plant / Q000032:analysis*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D032267:Rehmannia / Q000145:classification / Q000235:genetics*
D018744:DNA, Plant; D012336:RNA, Ribosomal, 16S