A NH2 tau fragment targets neuronal mitochondria at AD synapses: possible implications for neurodegeneration.
Synapses are ultrastructural sites for memory storage in brain, and synaptic damage is the best pathologic correlate of cognitive decline in Alzheimer's disease (AD). Post-translational hyperphosphorylation, enzyme-mediated truncation, conformational modifications, and aggregation of tau protein into neurofibrillary tangles (NFTs) are hallmarks for a heterogeneous group of neurodegenerative disorders, so-called tauopathies. AD is a secondary tauopathy since it is pathologically distinguished by the presence of amyloid-beta (Abeta)-containing senile plaques and the presence of tau-positive NFTs in the neocortex and hippocampus. Here, we report that a 20-22 kDa NH2-truncated tau fragment is largely enriched in human mitochondria from cryopreserved synaptosomes of AD brains and that its amount in terminal fields correlates with the pathological synaptic changes and with the organelle functional impairment. This NH2-truncated tau form is also found in other human, not AD-tauopathies, while its presence in AD patients is linked to Abeta multimeric species and likely to pathology severity. Finally native, patient-derived, Abeta oligomers-enriched extracts likely impair the mitochondrial function by the in vitro production of 20-22 kDa NH2-tau fragments in mature human SY5Y and in rat hippocampal neurons. Thus our findings suggest that the mitochondrial NH2-derived tau peptide distribution may exacerbate the synapse degeneration occurring in tauopathies, including AD, and sustain the in vivo NH-2 tau cleavage inhibitors as an alternative drug discovery strategies for AD therapy.
Amadoro
Giuseppina
G
Institute of Neurobiology and Molecular Medicine-CNR, IRCSS Fondazione Santa Lucia, Rome, Italy. g.amadoro@inmm.cnr.it
Corsetti
Veronica
V
Stringaro
Annarita
A
Colone
Marisa
M
D'Aguanno
Simona
S
Meli
Giovanni
G
Ciotti
Mariateresa
M
Sancesario
Giuseppe
G
Cattaneo
Antonino
A
Bussani
Rossana
R
Mercanti
Delio
D
Calissano
Pietro
P
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D009410:Nerve Degeneration / Q000378:metabolism* / Q000473:pathology; D009447:Neuroblastoma; D016874:Neurofibrillary Tangles / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013569:Synapses / Q000378:metabolism* / Q000473:pathology; D016875:tau Proteins / Q000378:metabolism*
C054369:MAPT protein, human; C489131:Mapt protein, rat; D010446:Peptide Fragments; D016875:tau Proteins