USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories.
The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.
Nour-Eldin
Hussam H
HH
Department of Plant Biology and Biotechnology, and VKR Centre Pro-Active Plants, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
Geu-Flores
Fernando
F
Halkier
Barbara A
BA
D019677:Artificial Gene Fusion / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D007753:Laboratories; D016133:Polymerase Chain Reaction
D004247:DNA