Subcellular localization of V2 protein of Tomato leaf curl Java virus by using green fluorescent protein and yeast hybrid system.
Tomato leaf curl Java virus-A (ToLCJV-A[ID]) from Southeast Asia is a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. Previously, we have elucidated the role of V1 ORF encoded by ToLCJV-A[ID] in cell-to-cell movement. In this study, the role of V2 (PreCP) in localization was determined. Subcellular localization of ToLCJV-A[ID] V2 in plant tissues showed that this protein is co-localized to the cell cytoplasm, perinuclear and associated with the endoplasmic reticulum network. The results obtained from deletion analysis indicate that fusion of N-terminal part of the V2, containing the nuclear export signals (NES), directed the accumulation of fluorescence towards the cell cytoplasm. Furthermore, functionality of the NES ((20)LAVKYLQLV(29)) in the N-terminal part of the V2 protein was confirmed by one-hybrid yeast system. Taken together, these results suggest that V2 enhances the coat protein-mediated nuclear export of ToLCJV-A[ID] and is consistent with the model in which V2 mediates viral DNA export from the nucleus to the plasmodesmata.
Sharma
Pradeep
P
Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. neprads@gmail.com
Gaur
Rajarshi K
RK
Ikegami
Masato
M
D001483:Base Sequence; D053839:Begomovirus / Q000378:metabolism*; D002678:Chimera / Q000378:metabolism; D022083:Cytoplasmic Structures / Q000821:virology; D004279:DNA, Viral / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000821:virology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000166:cytology / Q000821:virology; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000166:cytology / Q000821:virology; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D004279:DNA, Viral; D014764:Viral Proteins; D049452:Green Fluorescent Proteins