title: Apoptosis induced by hematoporphyrin monomethyl ether combined with He-Ne laser irradiation in vitro on canine breast cancer cells.
abstract: Hematoporphyrin monomethyl ether (HMME) is a novel and promising porphyrin-related photosensitizer for photodynamic therapy (PDT). The aim of this study was to investigate HMME-induced apoptosis in CHMm cells, a canine breast cancer cell line. CHMm cells were treated with HMME and a He-Ne laser at a wavelength of 632.8 nm. Cell viability was determined using the trypan blue exclusion assay. Apoptosis was analyzed using Hoechst 33258, AO/EB, Annexin V/PI staining and single-cell gel electrophoresis (comet assay). Apoptotic morphology was further confirmed by Giemsa staining and transmission electron microscopy. Rates of apoptosis increased following PDT-HMME treatment in a time-dependent manner. Taken together, these results demonstrated that apoptosis plays a major role in PDT-HMME-induced reduction in the viability of CHMm cells.
authors.affiliation: Department of Veterinary Clinic, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Xing Q
authors.initials: XQ
authors.lastname: Ma
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Hua T
authors.initials: HT
authors.lastname: Li
authors.forename: Rong R
authors.initials: RR
authors.lastname: Zhang
authors.forename: Xiao L
authors.initials: XL
authors.lastname: Ren
authors.forename: Hong B
authors.initials: HB
authors.lastname: Wang
authors.forename: Damu
authors.initials: D
authors.lastname: Tang
authors.forename: Wen R
authors.initials: WR
authors.lastname: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy / Q000379:methods; D004283:Dog Diseases / Q000188:drug therapy* / Q000601:surgery; D004285:Dogs; D005260:Female; D006371:Helium; D006415:Hematoporphyrins / Q000627:therapeutic use*; D054020:Lasers, Gas / Q000627:therapeutic use*; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy* / Q000601:surgery; D009356:Neon; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use*
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C494379:hematoporphyrin monomethyl ether; D006371:Helium; D009356:Neon