title: The effect of long-term control of reflux by fundoplication on aberrant deoxyribonucleic acid methylation in patients with Barrett esophagus.
abstract: OBJECTIVE
  We investigated the relationship between reflux and aberrant deoxyribonucleic acid (DNA) methylation, comparing methylation in the columnar epithelium following successful fundoplication to that in subjects with a failed fundoplication.
  
  
  BACKGROUND
  Gastroesophageal reflux is the main risk factor for Barrett esophagus and adenocarcinoma. In these diseases, there is a high level of DNA methylation.
  
  
  METHODS
  We enrolled 41 patients with Barrett esophagus and a fundoplication at least 5 years earlier for a 24-hour pH study, endoscopy, and collection of biopsies. Biopsies were obtained from 17 Barrett esophagus subjects who had not undergone esophageal surgery.
  
  
  RESULTS
  At the time of the study, 31 subjects were pH normal, 10 abnormal. Columnar biopsies were collected from 21 of the pH normal and 9 pH abnormal subjects, and all no surgery subjects. Complete regression of columnar mucosa was seen in 7 subjects with pH normal and 1 with pH abnormal. The length of Barrett esophagus did not differ between groups preoperatively, but was significantly less at the time of the study in the pH normal compared with pH abnormal or no surgery groups. Significantly, fewer genes were methylated in the pH normal than the pH abnormal or no surgery groups, which did not differ from each other. The number of methylated genes correlated with increased reflux, intestinal metaplasia, and increased columnar-lined esophagus length, but not acid-suppression medication.
  
  
  CONCLUSIONS
  Fundoplication that reduces reflux to normal levels can lead to regression of the columnar mucosa. Reflux is associated with aberrant DNA methylation, and control of reflux reduces deleterious genomic changes associated with cancer.
authors.affiliation: Discipline of Surgery, The University of Adelaide, Adelaide, Australia. eric.smith@adelaide.edu.au
authors.forename: Eric
authors.initials: E
authors.lastname: Smith
authors.forename: John J
authors.initials: JJ
authors.lastname: Kelly
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Ruskiewicz
authors.forename: Thomas
authors.initials: T
authors.lastname: Sullivan
authors.forename: Glyn G
authors.initials: GG
authors.lastname: Jamieson
authors.forename: Paul A
authors.initials: PA
authors.lastname: Drew
mesh_terms: D001471:Barrett Esophagus / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D001706:Biopsy; D019175:DNA Methylation / Q000502:physiology*; D004947:Esophagus / Q000473:pathology; D005260:Female; D018662:Fundoplication; D005764:Gastroesophageal Reflux / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D008297:Male; D008875:Middle Aged