Characterization of two human lung adenocarcinoma cell lines by reciprocal chromosome painting.
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
Peng
Kun-Jing
KJ
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China.
Wang
Jin-Huan
JH
Su
Wei-Ting
WT
Wang
Xi-Cai
XC
Yang
Feng-Tang
FT
Nie
Wen-Hui
WH
D000034:Abortion, Veterinary; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D020223:Chromosome Painting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping