title: [Inside quality control for whole blood preservation performed at blood transfusion compatibility testing laboratory].
abstract: This study was aimed to establish the technique for preparation and storage of internal quality control pro-ducts by using existing blood sample resources of blood transfusion compatibility testing laboratory. 24 healthy blood donors with group A and RhD-positive were randomly selected, and 4 ml venous blood from these donors were collected, respectively. Based on the use of anticoagulant type, whether to add red blood cell preservation solution and the samples stored at room temperature for 1 or 2 hours daily, 24 specimens were randomly divided into 8 groups by using factorial design methodology. All samples in tube with cap were stored at 4 degrees C, and placed at room temperature for 1 or 2 hours daily. ABO, RhD blood group (recorded on the agglutination strength of the forward and reverse typing), IgM anti-B antibody titer, and free hemoglobin concentration in the supernatant for all samples were detected at 0, 7, 14, 21, 28, 35 days of products preservation. The results indicated that the red blood cell damage from the group used anticoagulants ACD-B and added the MAP red blood cell preservation solution and placed at room temperature 1 hour daily (recorded as A2B2C1 group) was kept minimal, and FHb concentration and FHb increments at each time point were the lowest (p < 0.01), the FHb concentration on 35th day was only (24.5 +/- 84.5) mg/L. There was no significant change of A antigen, D antigen and IgM anti-B antibody response activity and stability in A2B2C1 group during storage for 35 days (p > 0.05). In conclusion, blood transfusion compatibility testing laboratory can use A2B2C1 program established by this study to prepare relatively stable modified whole blood internal quality control products in the existing conditions, which can be effectively preserved and meet the requirements of internal quality control for blood transfusion compatibility testing.
authors.affiliation: Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Chun-Ya
authors.initials: CY
authors.lastname: Ma
authors.forename: Qian
authors.initials: Q
authors.lastname: Feng
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Guan
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Chen
authors.forename: Zi-Lin
authors.initials: ZL
authors.lastname: Lin
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Pan
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Qun
authors.initials: Q
authors.lastname: Luo
authors.forename: De-Qing
authors.initials: DQ
authors.lastname: Wang
mesh_terms: D000017:ABO Blood-Group System; D001331:Automation; D001782:Blood Donors; D001788:Blood Grouping and Crossmatching; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D006801:Humans; D011786:Quality Control
chemical_list: D000017:ABO Blood-Group System