Self-assembly-peptide hydrogels as tissue-engineering scaffolds for three-dimensional culture of chondrocytes in vitro.
The promising potential of a RAD-16 self-assembly-peptide hydrogel as a scaffold for tissue-engineered cartilage was investigated. Within 3 weeks of in vitro culture, chondrocytes within the hydrogel produced a high amount of GAG and type-II collagen, which are the components of cartilage-specific extracellular matrix (ECM). With the culture time increased, toluidine-blue staining for GAG and immuno-histochemistry staining for type-II collagen of the chondrocytes-hydrogel composites became more intense. Analysis of the gene expression of the ECM molecules also confirmed the chondrocytes in the peptide hydrogel maintained their phenotype within 3 weeks of in vitro culture.
Liu
Jingping
J
Nanomedicine Laboratory, West China Hospital, Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu, P. R. China.
Song
Hong
H
Zhang
Lanlan
L
Xu
Hongyan
H
Zhao
Xiaojun
X
D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology; D005109:Extracellular Matrix / Q000737:chemistry / Q000502:physiology; D015870:Gene Expression; D006025:Glycosaminoglycans / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D010455:Peptides / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D020100:Hydrogels; D010455:Peptides