[Activation of the bioluminescence of the sensor Escherichia coli strains used for detecting N-acyl-homoserine lactones in the presence of nitrofurans and NO generators].
Nitrofurans (nitrofurazone, nitrofurantoin, furazidin, nifuroxazide), and nitric oxide generators (sodium nitroprusside and isosorbide mononitrate) in subinhibitory concentrations were shown to significantly increase the bioluminescence of the sensor Escherichia coli strains used for detecting N-acyl-homoserine lactones, signaling molecules of Quorum Sensing (QS) regulatory systems. The highest activation of bioluminescence (up to 250-400 fold) was observed in the presence of nitrofurazone on E. coli DH5alpha biosensors containing lux-reporter plasmids pSB401 or pSB536. However, this activation was not specifically associated with the functioning of QS systems. We suggest that the effect observed results from a direct action of nitrofurans and NO donors on the process of bioluminescence. The data indicate the necessity of using the biosensors that make it possible to detect specific effects of substances tested on QS regulation.
Zaĭtseva
Iu V
IuV
Granik
V G
VG
Belik
A S
AS
Koksharova
O A
OA
Khmel'
I A
IA
D054742:Acyl-Butyrolactones / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D049449:Luminescence; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009581:Nitrofurans / Q000494:pharmacology*; D053038:Quorum Sensing / Q000187:drug effects*
D054742:Acyl-Butyrolactones; D020030:Nitric Oxide Donors; D009581:Nitrofurans