Induction of hexokinase I expression in normal and diabetic rats by a brassinosteroid isoform.
This study aimed to investigate the effect of the plant growth regulator 28-homobrassinolide (HB) on the hexokinase I (HK I) enzyme gene expression in the tissues of normal and streptozotocin-induced diabetic rats. Normal and diabetic rats were administered 50 microg of HB for 15 consecutive days. The tissues level of HK I mRNA expression was quantitated by PCR and densitometry analysis, HK I protein expression was quantitated by Western blot and densitometry analysis, localization of HK I was done by immunohistochemistry and HK enzyme activity was determined by coupled enzyme assay. Subchronic treatment of rats with HB enhanced HK I enzyme expression in diabetic rat compared to the control rat. From these experimental evidences, we came to the conclusion that HK I played a vital role in the regulation of blood sugar in streptozotocin-induced diabetic rats. A direct role for hexokinase enzyme activity in the control of diabetes is presented.
Muthuraman
P
P
Dept. of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Kalapet, Puducherry 605014, India.
Srikumar
K
K
D000818:Animals; D001483:Base Sequence; D055162:Biocatalysis; D015153:Blotting, Western; D002778:Cholestanones / Q000494:pharmacology*; D017931:DNA Primers; D003720:Densitometry; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004790:Enzyme Induction; D006593:Hexokinase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013311:Streptozocin
D002778:Cholestanones; D017931:DNA Primers; D012333:RNA, Messenger; D013311:Streptozocin; C070163:homobrassinolide; D006593:Hexokinase