A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms.
Recombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.
Santana
Margarida
M
Instituto de Recursos Naturales y Agrobiologia de Sevilla, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012 Seville, Spain.
Portillo
Maria Carmen
MC
Gonzalez
Juan M
JM
D000595:Amino Acid Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011506:Proteins; D011993:Recombinant Fusion Proteins