Identification of a linear epitope for Fc-binding in the mouse FcγRIII.
Fc receptors are transmembrane proteins, found on the surfaces of immune cells, that aid in the removal of foreign pathogens by binding to antibody-coated targets via the Fc regions of the antibodies. To identify sites on mouse FcgammaRIII (moFcgammaRIII) alpha-chain that bind to the Fc region, peptides derived from the proximal extracellular domain (EC2) of moFcgammaRIII alpha-chain corresponding to the homologous region of human FcgammaRIIIB alpha-chain were synthesized. Binding of mouse IgG to the different peptides was tested by Dot-blot assay. The effective peptide (119)SFFHNEKSVRYH(130) located in the putative C-C' loop of the EC2 domain was found to bind mouse IgG specifically with an affinity of approximately 5.58 x 10(-5) M and inhibit the binding of mouse IgG to the receptor. Such a functional peptide should be very useful for further understanding the IgG-FcgammaR interaction and development of FcR-targeting drugs.
Xi
Jun
J
School of Food Science and Technology, Henan University of Technology, Zhengzhou 450052, China.
Zhang
Li N
LN
Hu
Guang P
GP
Wang
Li
L
Qiao
Song L
SL
Guo
Jun Q
JQ
Lu
Qi Y
QY
Zhang
Gai P
GP
Yang
Yan Y
YY
D000818:Animals; D000915:Antibody Affinity; D001665:Binding Sites; D001667:Binding, Competitive; D019556:COS Cells; D002645:Chickens; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000276:immunology; D015151:Immunoblotting; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D017452:Receptors, IgG / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment
D000939:Epitopes; C485700:Fcgr3 protein, mouse; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009842:Oligopeptides; D010446:Peptide Fragments; D017452:Receptors, IgG; D011994:Recombinant Proteins