Effect of iron on matrix metalloproteinase inhibition and on the prevention of dentine erosion.
It is known that some metal salts can inhibit matrix metalloproteinase (MMP) activity, but the effect of iron has not been tested yet. On the other hand, it has recently been suggested that MMP inhibition might influence dentine erosion. Based on this, the aims of this study were: (1) to test in vitro the effect of FeSO(4) on MMP-2 and -9 activity, and (2) to evaluate in situ the effect of FeSO(4) gel on dentine erosion. MMP-2 and -9 activities were analysed zymographically in buffers containing FeSO(4) in concentrations ranging between 0.05 and 1.5 mmol/l or not. Volunteers (n = 10) wore devices containing bovine dentine blocks (n = 60) previously treated with the following gel treatments: FeSO(4) (1 mmol/l FeSO(4)), F (NaF 1.23%; positive control) and placebo (negative control). The gels were applied once and removed after 1 min. Erosion was performed extraorally with Coca-Cola 4 times per day for 5 min over 5 days. Dentine wear was evaluated by profilometry. The data were analysed by Kruskal-Wallis and Dunn's tests (p < 0.05). FeSO(4) inhibited both MMP-2 (IC(50) = 0.75 mmol/l) and MMP-9 (IC(50) = 0.50 mmol/l) activities. In the in situ experiment, the mean wear (+/- SD) found for the F gel (0.79 +/- 0.08 microm) was significantly reduced in more than 50% when compared to the placebo gel (1.77 +/- 0.33 microm), but the FeSO(4) gel completely inhibited the wear (0.05 +/- 0.02 mum). Since FeSO(4) was able to inhibit MMP in vitro, it is possible that the prevention of dentine wear by the FeSO(4) gel in situ might be due to MMP inhibition, which should be investigated in further studies.
Kato
M T
MT
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Leite
A L
AL
Hannas
A R
AR
Oliveira
R C
RC
Pereira
J C
JC
Tjäderhane
L
L
Buzalaf
M A R
MA
D000328:Adult; D000818:Animals; D002417:Cattle; D003804:Dentin / Q000201:enzymology / Q000473:pathology; D004311:Double-Blind Method; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D005782:Gels; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology; D018709:Statistics, Nonparametric; D014077:Tooth Erosion / Q000201:enzymology* / Q000517:prevention & control*; D055815:Young Adult
D005290:Ferric Compounds; D005782:Gels; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; C024823:ferric sulfate; D012969:Sodium Fluoride