title: A new method for designing degenerate primers and its use in the identification of sequences in Brachiaria showing similarity to apomixis-associated genes.
abstract: We developed a technique and a tool for degenerate primer design based on multiple local alignments employing the MEME algorithm supported with electronic PCR. The objective is to find adequate primers starting from sequences with poor global similarity. We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.
authors.affiliation: Centro Internacional de Agricultura Tropical (CIAT), Cali, Colombia.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gorrón
authors.forename: Fausto
authors.initials: F
authors.lastname: Rodríguez
authors.forename: Diana
authors.initials: D
authors.lastname: Bernal
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Rodriguez-Rojas
authors.forename: Adriana
authors.initials: A
authors.lastname: Bernal
authors.forename: Silvia
authors.initials: S
authors.lastname: Restrepo
authors.forename: Joe
authors.initials: J
authors.lastname: Tohme
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D035201:Brachiaria / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry*; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers