Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat.
The regulation of mitochondrial DNA (mtDNA) processes is slowly being characterized at a structural level. We present here crystal structures of human mitochondrial regulator mTERF, a transcription termination factor also implicated in replication pausing, in complex with double-stranded DNA oligonucleotides containing the tRNA(Leu)(UUR) gene sequence. mTERF comprises nine left-handed helical tandem repeats that form a left-handed superhelix, the Zurdo domain.
Jiménez-Menéndez
Nereida
N
Institut de Biologia Molecular de Barcelona, Parc Cientific de Barcelona, Barcelona, Spain.
Fernández-Millán
Pablo
P
Rubio-Cosials
Anna
A
Arnan
Carme
C
Montoya
Julio
J
Jacobs
Howard T
HT
Bernadó
Pau
P
Coll
Miquel
M
Usón
Isabel
I
Solà
Maria
M
D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D024101:Mitochondrial Proteins; D008958:Models, Molecular; D011487:Protein Conformation; D020080:Tandem Repeat Sequences
D050976:Basic-Leucine Zipper Transcription Factors; D004272:DNA, Mitochondrial; C105178:MTERF1 protein, human; D024101:Mitochondrial Proteins; D004247:DNA