title: Development and immunogenicity of a recombinant pseudorabies virus expressing Sj26GST and SjFABP from Schistosoma japonicum.
abstract: Recombinant pseudorabies virus (PRV) Bartha-K61 vaccine strains expressing Schistosoma japonicum 26kDa glutathione S-transferase (Sj26GST) and fatty acid binding protein (SjFABP), designated as rPRV/Sj26GST, rPRV/SjFABP and rPRV/Sj26GST-SjFABP, were constructed and evaluated for their ability to protect mice and sheep against S. japonicum challenge. Animals were given 2 intramuscular immunizations 3 weeks apart, and challenged with S. japonicum cercariae 4 weeks later. All mice vaccinated with recombinant virus developed specific anti-SWAP (soluble worm antigen preparation) antibody, splenocyte proliferative response and production of IFN-gamma and IL-2. Injection of rPRV/Sj26GST-SjFABP significantly increased levels of antibody, splenocyte proliferative response and production of IFN-gamma, compared with rPRV/Sj26GST and rPRV/SjFABP. These recombinant viruses have been shown to be safe for sheep. Challenge experiments showed worms and egg burdens were significantly reduced in animals immunized with recombinant PRVs. Most importantly, rPRV/Sj26GST-SjFABP dramatically enhanced protection with worm reduction and hepatic reduction of 39.3% and 45.5% respectively in mice, and 48.5% and 51.2% in sheep, while rPRV/Sj26GST and rPRV/SjFABP provided corresponding protection of only up to 23.7% and 27.2% in mice, and 29.0% and 35.5% in sheep. These results indicate that the multivalent vaccine for S. japonicum can produce significant specific immunity and protection, and that PRV Bartha-K61 is an effective live vector for an animal schistosomiasis japonica vaccine.
authors.affiliation: Institute of Military Veterinary, AMMS, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130062, Jilin Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Yujia
authors.initials: Y
authors.lastname: Zhai
authors.forename: Hongtao
authors.initials: H
authors.lastname: Jin
authors.forename: Limin
authors.initials: L
authors.lastname: Shang
authors.forename: Jingtao
authors.initials: J
authors.lastname: Men
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Shi
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhu
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gao
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D002522:Chlorocebus aethiops; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control*; D012756:Sheep; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014709:Vero Cells
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007376:Interleukin-2; D014612:Vaccines; D014614:Vaccines, Synthetic; D007371:Interferon-gamma