title: Structural changes in endometrial basal glands during menstruation.
abstract: OBJECTIVE
  To prospectively observe the changes occurring in endometrial glandular morphology during menstrual shedding and regeneration.
  
  
  METHODS
  Prospective observational study.
  
  
  METHODS
  The academic gynaecological endoscopy unit of a university teaching hospital. Population Thirteen patients investigated for a variety of benign, non-infective gynaecological disorders during the active bleeding phase of the menstrual cycle.
  
  
  METHODS
  The morphological appearances of concurrent histological and scanning electron microscopic images of endometrium taken at different stages of the active bleeding phase of menstruation were studied and correlated with the simultaneous immunohistochemical expression of the Ki-67 proliferation marker and the CD68 marker of macrophage activity.
  
  
  METHODS
  Change in morphology of endometrial glands at various stages of menstruation.
  
  
  RESULTS
  Endometrial glands within the basalis show evidence of apoptosis and associated macrophage activity immediately before and during menstruation. There is subsequent destruction and removal of old secretory glandular epithelial elements, and rapid replacement with new narrow glands lined with small epithelial cells. There is no evidence of mitotic cell division or expression of Ki-67 in the glandular cells during this replacement process, but there is evidence of marked macrophage activity prior to glandular cell loss.
  
  
  CONCLUSIONS
  Early endometrial epithelial repair after menstruation is not a consequence of mitotic cell division. It occurs without evidence of Ki-67 expression. There is structural evidence of programmed cell death and intense macrophage activity associated with glandular remodelling. Dead epithelial cells are shed from the glands and accumulate within the endometrial cavity to be replaced by new small epithelial cells that appear to arise by differentiation of the surrounding stromal cells. We propose that these stromal cells are endometrial progenitor/stem cells.
authors.affiliation: Department of Gynaecology, University of Western Australia, Perth, Australia. raygarry@btinternet.com
authors.forename: R
authors.initials: R
authors.lastname: Garry
authors.forename: R
authors.initials: R
authors.lastname: Hart
authors.forename: K A
authors.initials: KA
authors.lastname: Karthigasu
authors.forename: C
authors.initials: C
authors.lastname: Burke
mesh_terms: D017209:Apoptosis / Q000502:physiology; D049109:Cell Proliferation; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008598:Menstruation; D008855:Microscopy, Electron, Scanning; D011446:Prospective Studies; D013234:Stem Cells / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology
chemical_list: D019394:Ki-67 Antigen