title: Expression of the plasma membrane markers aquaporin 1 (AQP1), glucose transporter 1 (GLUT1) and Na, K-ATPase in canine mammary glands and mammary tumours.
abstract: This study investigated the expression of the plasma membrane markers aquaporin 1 (AQP1), glucose transporter 1 (GLUT1) and the alpha1 subunit of Na, K-ATPase in normal canine mammary glands and in benign and malignant mammary tumours, using immunohistochemistry and semi-quantitative histomorphometry. AQP1 immunoreactivity was absent from the majority of specimens studied. GLUT1 immunoreactivity was observed in normal mammary tissue and particularly in the epithelial and mesenchymal cells of benign, and in the epithelial cells of malignant tumours, respectively. Na, K-ATPase immunoreactivity was present in normal and neoplastic mammary epithelium and was significantly increased in the epithelium of both benign and malignant tumours. These results suggest that GLUT1 is more highly expressed in neoplastic epithelium and mesenchyme and that Na, K-ATPase is more highly expressed in neoplastic mammary epithelium. In consequence, these membrane proteins may have potential as diagnostic and prognostic biomarkers of canine mammary neoplasia.
authors.affiliation: Small Animal Teaching Hospital, School of Veterinary Science, University of Liverpool, Leahurst Campus, Leahurst, Neston, Wirral CH64 7TE, UK.
authors.forename: Alistair
authors.initials: A
authors.lastname: Freeman
authors.forename: Udo
authors.initials: U
authors.lastname: Hetzel
authors.forename: Peter
authors.initials: P
authors.lastname: Cripps
authors.forename: Ali
authors.initials: A
authors.lastname: Mobasheri
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D004285:Dogs; D005260:Female; D015870:Gene Expression; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000378:metabolism*; D015674:Mammary Neoplasms, Animal / Q000175:diagnosis / Q000378:metabolism*; D011379:Prognosis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D051272:Glucose Transporter Type 1; D051398:Aquaporin 1; D000254:Sodium-Potassium-Exchanging ATPase