Enhanced bioaccumulation of dietary contaminants in catfish with exposure to the waterborne surfactant linear alkylbenzene sulfonate.
Fish bioaccumulate a variety of contaminants and act as an exposure portal to the human consumer. Surfactants, known pharmaceutically to alter membrane permeability, change drug bioavailability and attenuate transporter function are also found in contaminant mixtures in the aquatic environment. The overall objective of this study was to determine if the surfactant C-12 linear alkylbenzene sulfonate (LAS) at environmentally relevant concentrations, alters the disposition and enhances bioaccumulation of co-exposed dietary xenobiotics in the catfish. Included for study were the carcinogen benzo(a)pyrene (BaP), pharmaceutical, ivermectin (IVM), and P-glycoprotein (P-gp) substrate rhodamine 123 (Rho-123), each exhibiting different dispositional footprints. Rho-123 transport into bile and membrane fluidity was examined in isolated perfused livers from control and LAS exposed catfish. Mass balance residue assessments were performed on catfish following in vivo exposure for 12 days to LAS in water at 0, 100 or 300 microg/L with 6 days of (3)H-IVM or (3)H-BaP gavage treatments. LAS at 1, 5 and 20 microM in the perfused liver, significantly decreased the transport of Rho-123 (1 microM) into bile by 18.6, 38.1 and 66.7%, respectively. Fluorescence anisotropy measurements demonstrated a 29.7% increase in fluidity at the (1 microM, 348 microg/L) LAS concentration. In vivo mass balance studies indicated that waterborne LAS (100 and 300 microg/L) increased the dietary dose remaining in fish by 39% and 78% for (3)H-IVM and 50 and 157% for (3)H-BaP. LAS at environmentally relevant concentrations altered the bioavailability and disposition of dietary xenobiotics in the catfish. Co-exposure with LAS increases xenobiotic bioaccumulation, potential toxicity of mixture components to the fish and the potential for residue transfer from fish to the consumer.
Tan
Xiaobing
X
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Yim
Sun-Young
SY
Uppu
Prasanna
P
Kleinow
Kevin M
KM
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000378:metabolism; D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism; D001646:Bile / Q000737:chemistry; D002397:Catfishes / Q000378:metabolism*; D005260:Female; D007559:Ivermectin / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D020112:Rhodamine 123 / Q000032:analysis / Q000378:metabolism; D013501:Surface-Active Agents / Q000633:toxicity*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000032:analysis / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017738:Alkanesulfonic Acids; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; C030641:alkylbenzyl sulfonic acid; D020112:Rhodamine 123; D001564:Benzo(a)pyrene; D007559:Ivermectin