Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A.
summary The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3' of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3' of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.
Sivakumaran
K
K
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Chen
M-H
MH
Roossinck
M J
MJ
Kao
C C
CC