title: A rapid and specific derivatization procedure to identify acyl-glucuronides by mass spectrometry.
abstract: A simple procedure is described to identify acyl-glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl-glucuronide from an extract. Glucuronides derived from carbamic acids, and alkyl- and aromatic amines, are inert to the derivatization reaction conditions, making the hydroxamic acid derivative a fingerprint for acyl-glucuronides.
authors.affiliation: Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer Global Research and Development, Groton, CT 06340, USA. alfin.vaz@pfizer.com
authors.forename: Alfin D N
authors.initials: AD
authors.lastname: Vaz
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bessire
authors.forename: Raman
authors.initials: R
authors.lastname: Sharma
authors.forename: Anne E
authors.initials: AE
authors.lastname: Hagen
mesh_terms: D002219:Carbamates / Q000737:chemistry; D020719:Glucuronides / Q000737:chemistry*; D006877:Hydroxamic Acids / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D002219:Carbamates; D020719:Glucuronides; D006877:Hydroxamic Acids; D019811:Hydroxylamine; C070766:carbamic acid