Novel family of antimicrobial peptides from the skin of Rana shuchinae.
So far numerous antimicrobial peptides have been characterized from amphibians. In this work, a new family of antimicrobial peptides, named shuchin, was purified and characterized from skin secretions of the frog, Rana shuchinae that lives in freezing mountains. Totally two members of shuchin (shuchin 1 and 2) were identified with the amino acid sequence of NALSMPRNKCNRALMCFG and NALSSPRNKCDRASSCFG, respectively. cDNAs encoding shuchins were cloned from the skin cDNA library of R. shuchinae. The precursors of shuchin are composed of 62 amino acid residues including the conserved signal peptides, acidic propieces, and mature antimicrobial peptides. Synthetic shuchins showed strong and broad antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus, and Bacillus cereus; MICs<12.5 microg/ml), Gram-negative bacteria (Escherichia coli, Bacillus dysenteriae, Pseudomonas aeruginosa; most MICs from 3.1 to 12.5 microg/ml), and yeast (Candida albicans; MICs of 6.25 microg/ml), but no hemolytic activity under the effective concentration, thereby provide more leading templates for designing novel anti-infection agents.
Zheng
Ruiqiang
R
Intensive Care Unit, Subei People's Hospital of Jiangsu Province, Yangzhou, Jiangsu 225001, China.
Yao
Bin
B
Yu
Haining
H
Wang
Hanjin
H
Bian
Jianmin
J
Feng
Feifei
F
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D005260:Female; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D053878:Hemolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D011498:Protein Precursors / Q000737:chemistry; D021382:Protein Sorting Signals / Q000235:genetics; D011817:Rabbits; D011898:Ranidae / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D012867:Skin / Q000378:metabolism*
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D053878:Hemolytic Agents; D020033:Protein Isoforms; D011498:Protein Precursors; D021382:Protein Sorting Signals