[Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography].
OBJECTIVE
To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.


METHODS
The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.


RESULTS
The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.


CONCLUSIONS
The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.
Liu
Zan
Z
China Pharmaceutical University, Nanjing 210038, China. lzan2000@163.com
Chou
Guixin
G
Wang
Zhengtao
Z
D000097:Acetonitriles / Q000737:chemistry; D000241:Adenosine / Q000032:analysis; D019659:Asteraceae / Q000737:chemistry*; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control; D013549:Sweetening Agents / Q000032:analysis
D000097:Acetonitriles; D002273:Carcinogens; D004365:Drugs, Chinese Herbal; D013549:Sweetening Agents; D000241:Adenosine; C032159:acetonitrile