title: Use of laser microdissection in the investigation of facial motoneuron and neuropil molecular phenotypes after peripheral axotomy.
abstract: The mechanism underlying axotomy-induced motoneuron loss is not fully understood, but appears to involve molecular changes within the injured motoneuron and the surrounding local microenvironment (neuropil). The mouse facial nucleus consists of six subnuclei which respond differentially to facial nerve transection at the stylomastoid foramen. The ventromedial (VM) subnucleus maintains virtually full facial motoneuron (FMN) survival following axotomy, whereas the ventrolateral (VL) subnucleus results in significant FMN loss with the same nerve injury. We hypothesized that distinct molecular phenotypes of FMN existed within the two subregions, one responsible for maintaining cell survival and the other promoting cell death. In this study, we used laser microdissection to isolate VM and VL facial subnuclear regions for molecular characterization. We discovered that, regardless of neuronal fate after injury, FMN in either subnuclear region respond vigorously to injury with a characteristic "regenerative" profile and additionally, the surviving VL FMN appear to compensate for the significant FMN loss. In contrast, significant differences in the expression of pro-inflammatory cytokine mRNA in the surrounding neuropil response were found between the two subnuclear regions of the facial nucleus that support a causative role for glial and/or immune-derived molecules in directing the contrasting responses of the FMN to axonal transection.
authors.affiliation: Neuroscience Program, Loyola University Medical Center, Maywood, IL 60153, USA. nichole.mesnard@gmail.com
authors.forename: Nichole A
authors.initials: NA
authors.lastname: Mesnard
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Alexander
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Sanders
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Jones
mesh_terms: D000818:Animals; D019771:Axotomy / Q000295:instrumentation / Q000379:methods; D004195:Disease Models, Animal; D005154:Facial Nerve / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D053685:Laser Therapy / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D042282:Microdissection / Q000295:instrumentation / Q000379:methods*; D009046:Motor Neurons / Q000378:metabolism* / Q000473:pathology; D019581:Neuropil / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D017397:Prefrontal Cortex / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012183:Retrograde Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020651:Ventral Thalamic Nuclei / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology