title: Bioavailability of lysine for kittens in overheated casein is underestimated by the rat growth assay method.
abstract: Growth assays were performed to determine lysine bioavailability for kittens and rats in untreated and heated casein; these values were compared with estimates obtained with an in vitro method. Body weight, food intake, nitrogen and dry matter digestibility, and plasma lysine were determined during an 80-day growth trial using kittens (n = 16). Body weight and food intake were determined during a 21-day growth trial using weanling rats (n = 80). The growth data showed bioavailable lysine to be 102.4% and 100.2% (for untreated casein) and 66.1% and 51.7% (for heated casein) for kittens and rats, respectively. There was no relationship between plasma lysine and dietary lysine concentrations for kittens. There were no significant differences in nitrogen or dry matter digestibility among diets for kittens. The chemically reactive lysine content of untreated casein was 99.6%, and of heated casein was 67.1%. Heat treatment of casein resulted in significantly decreased lysine bioavailability as estimated by all methods. For untreated casein, both growth assays showed good agreement with the in vitro method for available lysine. For heated casein, the rat growth assay significantly underestimated bioavailable lysine as determined in kittens while the in vitro method closely approximated this value for the cat.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. jalarsen@vmth.ucdavis.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Larsen
authors.forename: A J
authors.initials: AJ
authors.lastname: Fascetti
authors.forename: C C
authors.initials: CC
authors.lastname: Calvert
authors.forename: Q R
authors.initials: QR
authors.lastname: Rogers
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001681:Biological Assay; D001682:Biological Availability; D002364:Caseins / Q000737:chemistry*; D002415:Cats / Q000254:growth & development*; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins; D004063:Digestion; D005511:Food Handling; D006358:Hot Temperature; D008239:Lysine / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D013047:Specific Pathogen-Free Organisms
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D008239:Lysine