Supramolecular recognition of estrogens via molecularly imprinted polymers.
The isolation and preconcentration of estrogens from new types of biological samples (acellular and protein-free simulated body fluid) by molecularly imprinted solid-phase extraction has been described. In this technique, supramolecular receptors, namely molecularly imprinted polymers (MIPs) are used as a sorbent material. The recognition sites of MIPs were prepared by non-covalent multiple interactions and formed with the target 17beta-estradiol as a template molecule. High-performance liquid chromatography with spectroscopic UV, selective, and a sensitive electrochemical CoulArray detector was used for the determination of 17beta-estradiol, estrone, and estriol in simulated body fluid which mimicked human plasma.
Buszewski
Bogusław
B
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St, 87 100 Toruń, Poland. bbusz@chem.umk.pl
Ricanyová
Júlia
J
Gadzała-Kopciuch
Renata
R
Szumski
Michał
M
D000327:Adsorption; D004958:Estradiol / Q000737:chemistry / Q000302:isolation & purification; D004967:Estrogens / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation*
D004967:Estrogens; D011108:Polymers; D004958:Estradiol