Automated single nucleotide polymorphism typing using bead array in capillary tube.
A low-cost and simple on-site technique for genotyping single nucleotide polymorphisms (SNPs) was developed. The technique is based on allele-specific primer PCR and the recently developed bead arrays in a single tip technique. The performance of the method was verified by genotyping four SNPs that correlate with cardiovascular diseases.
Kagawa
Yasuo
Y
High Technology Research Center of Kagawa Nutrition University, 3-9-21 Chiyoda, Sakado, Saitama 350-0288, Japan. kagawa@eiyo.ac.jp
Hiraoka
Mami
M
Miyashita-Hatano
Yukiko
Y
Shishido-Oki
Madoka
M
Yoshida
Mamiko
M
Kondou
Susumu
S
Sugiura
Mika
M
Sawakami-Kobayashi
Kazumi
K
Takahashi
Masaaki
M
Tajima
Hideji
H
Yohda
Masafumi
M
D001331:Automation; D001483:Base Sequence; D017931:DNA Primers; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers