Synthesis of 4',7-diacetoxyapigenin and its apoptotic induction in human Hep G2 cells.
In this study, 4',7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, (1)H and (13)C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4',7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4',7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
Xu
Keyong
K
College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China; E-Mail: fezhning@scut.edu.cn (Z.N.).
Liu
Feng
F
Liu
Benguo
B
Gao
Han
H
Ning
Zhengxiang
Z
D047310:Apigenin / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016193:G1 Phase / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*
D047310:Apigenin