Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1.
CHD1 is a subfamily member of the CHD family, which possesses a chromodomain, a helicase domain, and a DNA-binding domain. The CHD family regulates gene expression by contributing to ATP-dependent chromatin remodeling. CHD1 exists in the transcriptionally active region and alters the chromatin structure. Little is known about the function of endogenous CHD1, however, and studies have been hindered by the lack of an antibody specific for CHD1 in mammals. In the present study, we established a monoclonal antibody specifically against CHD1 using the rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody showed specific binding to CHD1, allowing us to identify the deduced full-length CHD1. In addition, cell immunostaining clearly revealed the nuclear localization of CHD1. This monoclonal antibody will be useful for further analysis of CHD1 function in mammals.
Yoshimura
Saori
S
Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
Harada
Akihito
A
Odawara
Jun
J
Azuma
Masayuki
M
Okada
Seiji
S
Nakamura
Mako
M
Ohkawa
Yasuyuki
Y
Tachibana
Taro
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000276:immunology*; D042002:Chromatin Assembly and Disassembly; D004265:DNA Helicases / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D007082:Ileum / Q000276:immunology; D007114:Immunization; D015151:Immunoblotting; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D041681:NIH 3T3 Cells; D051381:Rats; D011921:Rats, Inbred WKY
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D004265:DNA Helicases; C080359:CHD1 protein, human