title: [Effect of Dureping injection on TIR signal pathway on Ana-1 cells].
abstract: OBJECTIVE
  To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.
  
  
  METHODS
  Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR.
  
  
  RESULTS
  Dureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly.
  
  
  CONCLUSIONS
  Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.
authors.affiliation: Department of Etiology and Immunology of Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ningping
authors.initials: N
authors.lastname: Wang
authors.forename: Ligang
authors.initials: L
authors.lastname: Gu
authors.forename: Pengtao
authors.initials: P
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002477:Cells; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D048029:TNF Receptor-Associated Factor 6 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6; D051996:Transcription Factor RelA; C507773:IRAK4 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases