The chemical toolbox for monitoring protein fatty acylation and prenylation.
Lipid modification of cellular proteins plays diverse roles in the regulation of such proteins' trafficking, signaling and behavior. Owing to a lack of robust detection technologies, the mechanisms by which lipids regulate proteins are poorly understood. Recently, various groups have developed innovative chemical probes in conjunction with bio-orthogonal chemistry for the detection of lipid-modified proteins in vitro and in vivo. These new methods enable further understanding of the mechanisms of protein lipidation and its function in physiology and disease. Here we present a comprehensive summary of these detection probes for monitoring fatty acylation and prenylation, and we provide a perspective on their current and future applications.
Hannoush
Rami N
RN
Department of Protein Engineering, Genentech, Inc., South San Francisco, California, USA. hannoush.rami@gene.com
Sun
Jinglucy
J
D000215:Acylation; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D054876:Prenylation; D011506:Proteins / Q000378:metabolism*
D008055:Lipids; D011506:Proteins