[Development of two-dimensional micro strong cation exchange liquid chromatography/reversed phase pressurized capillary electrochromatography and its application on Cortex Phellodendri extract].
Pressurized capillary electrochromatography (pCEC) combines capillary electrophoresis (CE) with high performance liquid chromatography (HPLC), resulting in high separation efficiency, high selectivity, high resolution and fast speed. In addition, it provides a solvent gradient capability. Based on pCEC, a two-dimensional chromatography was constructed using micro strong cation exchange liquid chromatography and reversed phase pressurized capillary electrochromatography (micro-SCXLC/RP-pCEC) for the analysis of Cortex Phellodendri extract. A sample was separated by the first dimensional SCX column with a linear salt gradient elution into 11 plugs. Each plug was collected and injected into the second dimensional RP-pCEC column with a linear gradient for further separation. Compared with one-dimensional liquid chromatography, it has higher resolution and larger peak capacity. The two-dimensional chromatographic system is suitable for the separation and analysis of complex samples.
Wu
Yi
Y
Shanghai Jiao Tong University, Shanghai 200240, China.
Wang
Yan
Y
Gu
Xue
X
Zhang
Lin
L
Yan
Chao
C
D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D032102:Phellodendron / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts