title: Natural ligand binding and transfer from liver fatty acid binding protein (LFABP) to membranes.
abstract: Liver fatty acid-binding protein (LFABP) is distinctive among fatty acid-binding proteins because it binds more than one molecule of long-chain fatty acid and a variety of diverse ligands. Also, the transfer of fluorescent fatty acid analogues to model membranes under physiological ionic strength follows a different mechanism compared to most of the members of this family of intracellular lipid binding proteins. Tryptophan insertion mutants sensitive to ligand binding have allowed us to directly measure the binding affinity, ligand partitioning and transfer to model membranes of natural ligands. Binding of fatty acids shows a cooperative mechanism, while acyl-CoAs binding presents a hyperbolic behavior. Saturated fatty acids seem to have a stronger partition to protein vs. membranes, compared to unsaturated fatty acids. Natural ligand transfer rates are more than 200-fold higher compared to fluorescently-labeled analogues. Interestingly, oleoyl-CoA presents a markedly different transfer behavior compared to the rest of the ligands tested, probably indicating the possibility of specific targeting of ligands to different metabolic fates.
authors.affiliation: INIBIOLP, Facultad de Ciencias Médicas, Universidad Nacional de La Plata. Calle 60 y 120, 1900, La Plata, Argentina.
authors.forename: Eduardo
authors.initials: E
authors.lastname: De Gerónimo
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hagan
authors.forename: David C
authors.initials: DC
authors.lastname: Wilton
authors.forename: Betina
authors.initials: B
authors.lastname: Córsico
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D008024:Ligands; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D008024:Ligands; D011994:Recombinant Proteins; C017585:oleoyl-coenzyme A