title: Chromium(VI) oxide oxidation of non-ethoxylated and ethoxylated alcohols for determination by electrospray ionization mass spectrometry.
abstract: A new derivatization procedure to increase the sensitivity of electrospray ionization mass spectrometry (ESI-MS) to non-ethoxylated and ethoxylated alcohols was investigated. The analytes were oxidized with chromium(VI) oxide and the resulting carboxylic and ethoxy-carboxylic acids were isolated by extraction with ethyl acetate; the extracts were alkalinized and infused into the ESI-MS system working in the negative-ion mode. The yields of the combined oxidation-extraction were ca. 100% for non-ethoxylated fatty alcohols dissolved in acetone and they decreased moderately in samples containing increasing amounts of water (e.g., a 75% yield was obtained with 50% water). Ethoxylated alcohols with more than two ethylene oxide units resulted in yields of ca. 60%. Low limits of detection (LODs) were obtained when the procedure was applied to the analysis of body-care products and cosmetics containing fatty alcohols, e.g., in a varicose-vein cream, the LODs were 25 microg cetyl alcohol and 7.5 microg stearyl alcohol (detected as palmitic acid and stearic acid, respectively) per gram of sample. High molecular mass alcohols were also detected in seawater after pre-concentration by solid-phase extraction. Thus, the proposed method is particularly valuable for use in industrial samples having complex matrices and in environmental samples and it is competitive with other methods for the analysis of trace amounts of fatty alcohols.
authors.affiliation: Departament de Química Analítica, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Beneito-Cambra
authors.forename: Virginia
authors.initials: V
authors.lastname: Bernabé-Zafón
authors.forename: Ernesto F
authors.initials: EF
authors.lastname: Simó-Alfonso
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis-Ramos
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D017608:Chromium Compounds / Q000737:chemistry*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000438:Alcohols; D017608:Chromium Compounds; C023600:chromic oxide