[Quality control for ABO blood group typing of neonatal umbilical cord blood].
This study was aimed to investigate a quality control method for ABO typing of neonatal umbilical cord blood(UCB). The routine serology method was used to identify the ABO type of UCB samples. These samples with questions were further detected by sequence specific primer PCR (PCR-SSP). The results showed that among total of 76120 UCB samples identified by positive ABO typing, there were 78 samples (1 per thousand) which could not be determined. Of these 78 samples, 30 (56.92%) samples with a weak agglutination reaction were excluded by reverse ABO typing. Out of 260 samples in reverse ABO typing, 148 samples were consistent with positive ABO typing, 112 samples (43.08%) were inconsistent with the positive ABO typing. 58 undetermined samples were detected by PCR-SSP. Out of them the genotyping results of 45 samples confirmed the serological typing, the phenotyping results in 3 cases were inconsistent to that of genotyping. 10 cases showed the unconformity between positive and reverse typing, but the genotyping results were fully consistent with the positive typing. In conclusion, positive typing for red cell antigens combined with PCR-SSP is efficient and sensitive for quality control of ABO typing for neonatal UCB.
Liu
Lan-Ting
LT
Union Stem Cell & Gene Engineering Co., Ltd., Tianjin Cord Blood Bank, Tianjin 300384, China.
Liang
Xiao-Lan
XL
Han
Jun-Ling
JL
Li
Qian
Q
Qiu
Lu-Gui
LG
Yu
Li-Jia
LJ
Sun
Le-Jing
LJ
DU
Ying
Y
D000017:ABO Blood-Group System / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D005312:Fetal Blood; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D011786:Quality Control
D000017:ABO Blood-Group System