title: Chromatin remodeling protein INO80 has a role in regulation of homeotic gene expression in Drosophila.
abstract: The homologues of yeast INO80 are identified across phyla from Caenorhabditis elegans to human. In Drosophila it has been shown that dINO80 forms a complex with Pleiohomeotic but does not interact with Hox PRE (polycomb responsive element). As some proteins of the INO80 complex are implicated in homeotic gene regulation, we examined if dINO80 is involved in regulation of homeotic genes. We find that dINO80 null mutants generated by imprecise excision of P-element are late embryonic lethals and show homeotic transformation. We detect misexpression of homeotic genes like Sex-comb reduced, Antennapedia, Ultrabithorax and Abdominal-B in dIno80 mutant embryos by immunostaining which is further substantiated by quantitative PCR. Polycomb phenotype in dIno80-Pc is enhanced in double mutants. Concurrently, the localization of dINO80 to sequences upstream of misexpressed genes in vivo shows that dINO80 is involved in homeotic gene regulation and probably through its interactions with PcG-trxG complexes.
authors.affiliation: Dr.B.R.Ambedkar Center for Biomedical Research, University of Delhi, India.
authors.forename: Shipra
authors.initials: S
authors.lastname: Bhatia
authors.forename: Hema
authors.initials: H
authors.lastname: Pawar
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Dasari
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Mishra
authors.forename: Shanti
authors.initials: S
authors.lastname: Chandrashekaran
authors.forename: Vani
authors.initials: V
authors.lastname: Brahmachari
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; C553462:INO80 protein, Drosophila; D012333:RNA, Messenger; D014157:Transcription Factors