Induction of micronuclei on Greek hairdressers occupationally exposed to chemical mixtures.
Since the early 20th century, hairdressers (HD) have been exposed to a wide range of harmful chemical products. To determine the possible genetic damage to HD, as a result of their occupational exposure to combinations of different chemical factors, we applied the micronucleus assay on their peripheral blood lymphocytes cultures. The micronucleus assay was performed on blood samples from 20 Greek female HD and 20 control women, having no connection with the occupation, from the same area. In the results analysis, parameters included were age, smoking habits, and duration of occupational exposure. The results of our study showed a significant increase in HD micronuclei (MN) frequency, compared to the controls (13.4 ± 1.00 vs. 8.05 ± 0.65). The frequency of large-size MN was significantly higher in the HD and presented potential correlation with the phenomenon of aneuploidy. A statistically significant difference in the frequency of MN between HD and controls who smoked was observed, while this was not the case with the non smoker groups. However, multiple regression analysis showed no significant correlation between smoking habits and MN frequency. The observed increase of the frequency of MN in HD is attributed to the long-term occupational exposure of HD in combination with different chemical factors. Since in the literature there are very few similar studies, further combined studies are suggested on a larger number of HD from different countries, combining biological and molecular techniques, as well as chemical analytical methods of determining and tracing the chemical factors in both the occupational environment and their organisms.
Vlastos
Dimitris
D
Department of Environmental and Natural Resources Management, University of Ioannina, Agrinio, Greece. dvlastos@cc.uoi.gr
Ntinopoulos
Alexandros
A
D000328:Adult; D001505:Beauty Culture; D005260:Female; D006115:Greece; D006203:Hair Preparations / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D016273:Occupational Exposure / Q000009:adverse effects*; D009790:Occupations; D012044:Regression Analysis; D012907:Smoking / Q000009:adverse effects; D055815:Young Adult
D006203:Hair Preparations