[Expression of transcription nuclear factor NFkappaB in laryngeal carcinoma tumor cells--correlation with IL-10 expression by blood mononuclear cells and clinicomorphological features].
OBJECTIVE
Transcription nuclear factor NFkappaB (p65-p50/RelA) is an activator of transcription process and regulates the apoptosis, cell cycle, proliferation, secretion of cytokines, angiogenic and growth factors by initiation gene transcription. NFkappaB can be activated by signaling pathway following IL-10 stimulation. The aim of this study was to estimate NFkappaB cytoplasmic and nuclear immunoexpression and to analyze the connection with clinicopathological features and IL-10 concentration produced by blood mononuclear cells in patients with laryngeal carcinoma.


METHODS
Analysis of NFkappaB expression by immunmohistochemistry and IL-10 production by PBMCs and measured by Elisa in 45 patients with squamous cell carcinoma of the larynx were performed. Connections NFkappaB immunoexpression with clinicomorphological features (pT, pN, Anneroth, Batsakis i Lunas' classification) and IL-10 secretion were analyzed.


RESULTS
Authors reported statistical correlation between NFkappaB cytoplasmic level and clinicomorphological parameters such as neoplasm advance according to Anneroth, Batsakis i Lunas' classification and depth of invasion as well as IL-10 secretion by PBMCs.


CONCLUSIONS
Our studied indicated the important influence of NFkappaB activity on advance in laryngeal carcinoma as well as connection of anti-inflammatory and regulatory cytokine IL-10 produced by immunocompetent cells for course of neoplasm disease.
Starska
Katarzyna
K
Klinika Laryngologii Onkologicznej UM w Łodzi. katarzyna.starska@op.pl
Stasikowska
Olga
O
Lewy-Trenda
Iwona
I
Głowacka
Ewa
E
Łukomski
Marek
M
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000097:blood*; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D051996:Transcription Factor RelA / Q000378:metabolism*
D014408:Biomarkers, Tumor; D051996:Transcription Factor RelA; D016753:Interleukin-10