title: Modulation of miR-122 on persistently Borna disease virus infected human oligodendroglial cells.
abstract: Using RNAhybrid software we found the predicted binding of complementary sequences between miR-122 and viral mRNAs, may be important for the antiviral effect of miR-122 on Borna disease virus (BDV). A moderate expression of miR-122 was identified in human oligodendroglial cells (OL), but with a much lower level of miR-122 in BDV persistent infection (OL/BDV) and cells transfected with BDV gene expression vectors. Over-expression of miR-122 and specific blocking experiments demonstrated that miR-122 was able to specifically inhibit BDV protein synthesis, viral gene replication and transcription, and induce the secretion/synthesis of interferon (IFN) in OL and OL/BDV cells. The abolishment of miR-122 by AMO-122 inhibited endogenous IFN induction by IFN-beta. These results indicate that miR-122 can exert direct antiviral function by inhibiting BDV translation and replication on one hand, while acting indirectly through IFN to increase the host innate immunity to modulate the virus-host interactions on the other hand.
authors.affiliation: Department of Microbiology, Harbin Medical University; Immunity and Infection, Pathogenic Biology Key Laboratory, Heilongjiang Province, Harbin 150086, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Aixia
authors.initials: A
authors.lastname: Zhai
authors.forename: Wenping
authors.initials: W
authors.lastname: Kao
authors.forename: Yujun
authors.initials: Y
authors.lastname: Li
authors.forename: Wuqi
authors.initials: W
authors.lastname: Song
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Fu
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Chen
authors.forename: Qingmeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhong
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
mesh_terms: D001891:Borna disease virus / Q000276:immunology* / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D006801:Humans; D007372:Interferons / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D009836:Oligodendroglia / Q000276:immunology* / Q000821:virology*; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D014764:Viral Proteins; D007372:Interferons