Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice.
Airway inflammation and mucus hyperproduction play the central role in the development of asthma, although the mechanisms remain unclear. The aquaporin (AQP)-5 may be involved in the process due to its contribution to the volume of liquid secreted from the airways. The present study firstly found the overexpression of AQP5 in the airway epithelium and submucosal glands of asthmatics. Furthermore, we aimed at evaluating the role of AQP5 in airway inflammation and mucous hyperproductions during chronic allergic responses to house dust mite (HDM). Bronchoalveolar lavage levels of interleukin (IL)-2, IL-4, IL-10, interferon-γ and Mucin 5AC (MUC5AC), and number of peribronchial and perivascular cells were measured in AQP5 wild-type and AQP5 knockout (KO) mice. We found that HDM induced airway inflammation, lung Th2 cell accumulation and mucin hypersecretion in C57BL/6 mice rather than AQP5 KO mice. Expression of MUC5AC and MUC5B proteins and genes in the lung tissue was significantly lower in AQP5 KO mice. Thus, our results implicate involvement of AQP5 in the development of airway inflammation and mucous hyperproduction during chronic asthma.
Shen
Yao
Y
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Wang
Yaoli
Y
Chen
Zhihong
Z
Wang
Diane
D
Wang
Xiangdong
X
Jin
Meiling
M
Bai
Chunxue
C
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D051402:Aquaporin 5 / Q000172:deficiency / Q000235:genetics; D001249:Asthma / Q000276:immunology / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D017353:Gene Deletion; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055271:Mucin 5AC / Q000096:biosynthesis*; D055257:Mucin-5B / Q000096:biosynthesis*; D009093:Mucus / Q000276:immunology* / Q000378:metabolism; D039981:Pyroglyphidae / Q000276:immunology* / Q000378:metabolism; D012137:Respiratory System / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D051402:Aquaporin 5; D016207:Cytokines; C524679:Muc5ac protein, mouse; C524579:Muc5b protein, mouse; D055271:Mucin 5AC; D055257:Mucin-5B