[Complex effects of simulated acid rain and Cu on the physiological characteristics of Paulownia fortunei and its detoxification mechanism].
A pot experiment was conducted to study the effects of simulated acid rain (pH 4.0, 5.0) and Cu (0-200 mg x kg(-1)) on the physiological characteristics of Paulownia fortunei and its detoxification mechanism. With no Cu addition, the leaf chlorophyll, carotenoid, O2 division by, H2O2, and MDA contents of P. fortunei had no significant differences between the two acid rain treatments. However, with the addition of 100 and 200 mg Cu x kg(-1), the chlorophyll and carotenoid contents of treatment pH 4.0 were lower, while the O2 divided by, H2O2 and MDA contents were higher thanthose of treatment pH 5.0. The chlorophyll a/b ratio of treatments Cu was higher than that of the control. The leaf Cu content decreased obviously with the increasing acidity of stimulated acid rain, but the root Cu content was in reverse. With increasing Cu addition, both the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) and the total contents of phytochelatins (PCs) and glutathione (GSH) in treatment pH 5.0 increased, while the activities of SOD, POD, CAT and APX in treatment pH 4.0 decreased after an initial increase, and the total contents of glutathione (GSH) decreased greatly in treatment 200 mg Cu x kg(-1). All of these demonstrated that the oxidative stress of high Cu concentration to P. fortunei was aggravated by stimulated acid rain.
Wang
Jiang
J
School of Life Sciences, Taizhou University, Linhai 317000, Zhejiang, China. wangjiang@tzc.edu.cn
Zhang
Chong-Bang
CB
Ke
Shi-Sheng
SS
Qian
Bao-Ying
BY
D015258:Acid Rain / Q000009:adverse effects*; D001673:Biodegradation, Environmental; D003300:Copper / Q000378:metabolism / Q000633:toxicity*; D028083:Scrophulariaceae / Q000378:metabolism / Q000502:physiology*; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*
D015258:Acid Rain; D012989:Soil Pollutants; D003300:Copper