title: Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia.
abstract: Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination.
authors.affiliation: Department of Microbiology, University Hospital of Antwerp, Wilrijkstraat 10, Edegem, Belgium.
authors.forename: A A
authors.initials: AA
authors.lastname: Bankole
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
authors.forename: D
authors.initials: D
authors.lastname: Berkvens
authors.forename: D
authors.initials: D
authors.lastname: Fretin
authors.forename: S
authors.initials: S
authors.lastname: Geerts
authors.forename: G
authors.initials: G
authors.lastname: Ieven
authors.forename: K
authors.initials: K
authors.lastname: Walravens
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002002:Brucella / Q000145:classification* / Q000235:genetics; D002003:Brucella abortus / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000382:microbiology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005714:Gambia; D005838:Genotype; D010641:Phenotype; D012395:Rose Bengal
chemical_list: D012395:Rose Bengal