title: The mechanical properties of PCNA: implications for the loading and function of a DNA sliding clamp.
abstract: Sliding clamps are toroidal proteins that encircle DNA and act as mobile platforms for DNA replication and repair machinery. To be loaded onto DNA, the eukaryotic sliding clamp Proliferating Cell Nuclear Antigen (PCNA) must be splayed open at one of the subunit-subunit interfaces by the ATP-dependent clamp loader, Replication Factor C, whose clamp-interacting sites form a right-handed spiral. Earlier molecular dynamics (MD) studies suggested that when PCNA opens, it preferentially adopts a right-handed spiral to match the spiral of the clamp loader. Here, analysis of considerably longer MD simulations shows that although the opened form of PCNA can achieve conformations matching the helical pitch of Replication Factor C, it is not biased toward a right-handed spiral structure. A coarse-grained elastic model was also built; its strong correspondence to the all-atom MD simulations of PCNA suggests that the behavior of the open clamp is primarily due to elastic deformation governed by the topology of the clamp domains. The elastic model was further used to construct the energy landscape of the opened PCNA clamp, including conformations that would allow binding to the clamp loader and loading onto double-stranded DNA. A picture of PCNA emerges of a rather flexible protein that, once opened, is mechanically compliant in the clamp opening process.
authors.affiliation: Biophysics Graduate Group, University of California, Berkeley, California, USA.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Adelman
authors.forename: John D
authors.initials: JD
authors.lastname: Chodera
authors.forename: I-Feng W
authors.initials: IF
authors.lastname: Kuo
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Miller
authors.forename: Daniel
authors.initials: D
authors.lastname: Barsky
mesh_terms: D001696:Biomechanical Phenomena; D004247:DNA / Q000378:metabolism*; D004548:Elasticity; D056004:Molecular Dynamics Simulation; D009068:Movement; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D051818:Replication Protein C / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D051818:Replication Protein C