title: Micronuclei in the blood and bone marrow cells of mice exposed to specific complex time-varying pulsed magnetic fields.
abstract: For 8 weeks, adult CD-1 male mice were continuously exposed to complex time-varying pulsed magnetic fields (PMF) generated in the horizontal direction by a set of square Helmholtz coils. The PMF were <1000 Hz and delivered at a peak flux density of 1 mT. Sham-exposed mice were kept in a similar exposure system without a PMF. Positive control animals exposed to 1 Gy gamma radiation were also included in the study. Blood samples were collected before (time 0) and at 2, 4, 6, and 8 weeks. All mice were euthanized at the end of 8 weeks and their bone marrow was collected. From each blood and bone marrow sample, smears were prepared on microscope slides, fixed in absolute methanol, air-dried, and stained with acridine orange. All slides were coded and examined using a fluorescence microscope. The extent of genotoxicity and cytotoxicity was assessed from the incidence of micronuclei (MN) and percent polychromatic erythrocytes (PCE) in the blood and bone marrow, respectively. The data indicated that both indices in PMF-exposed mice were not significantly different from those observed in sham-exposed animals. In contrast, positive control mice exhibited significantly increased MN, and decreased percentages of PCE in both tissues. Thus, the overall data suggested that 8 weeks of continuous exposure to PMF did not induce significantly increased genotoxicity and cytotoxicity in experimental mice. Further investigations are underway using other genotoxicity assays (comet assay, gamma-H2AX foci, and chromosomal aberrations) to assess genotoxicity following PMF exposure.
authors.affiliation: Lawson Health Research Institute, London, Ontario, Canada.
authors.forename: Siddharth B
authors.initials: SB
authors.lastname: Reddy
authors.forename: Julie
authors.initials: J
authors.lastname: Weller
authors.forename: Dawn
authors.initials: D
authors.lastname: Desjardins-Holmes
authors.forename: Tara
authors.initials: T
authors.lastname: Winters
authors.forename: Lynn
authors.initials: L
authors.lastname: Keenliside
authors.forename: Frank S
authors.initials: FS
authors.lastname: Prato
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Prihoda
authors.forename: Vijayalaxmi
authors.initials: V
authors.lastname: Thomas
authors.forename: Alex W
authors.initials: AW
authors.lastname: Thomas
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D004912:Erythrocytes / Q000166:cytology / Q000528:radiation effects; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D013997:Time Factors