title: A promising biosensing-platform based on bismuth oxide polycrystalline-modified electrode: characterization and its application in development of amperometric glucose sensor.
abstract: Nano-structured bismuth oxide (nano-BiOx) is a suitable material for enzyme immobilization owing to its attractive properties, such as large specific surface area, suitable permeability of the resulting film, the high biocompatibility, and as well as photovoltaic effect from semiconductor nanoparticles. Thus, a new type of amperometric glucose biosensor based on nano-BiOx was constructed. The amperometric detection of glucose was assayed by potentiostating the GOD/nano-BiOx electrode at 0.5 V to oxidize the enzymatically generated hydrogen peroxide. The proposed biosensor provided a linear response to glucose over a concentration range of 1 x 10(-6) M to 1.5 x 10(-3) M with a sensitivity of 51.0+/-0.4 mA/(M cm(2)) and a detection limit of 4 x 10(-7) M based on S/N=3. The apparent Michaelis-Menten constant was calculated to be 2.9 x 10(-3) M. In addition, characterization of nano-BiOx and modified electrode was performed by FT-IR spectroscopy, Raman spectroscopy, scanning electron microscope (SEM) and rotating-disk electrode (RDE) voltammetry.
authors.affiliation: School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, China. snding@seu.edu.cn
authors.forename: Shou-Nian
authors.initials: SN
authors.lastname: Ding
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
authors.forename: Huai-Guo
authors.initials: HG
authors.lastname: Xue
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001729:Bismuth / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D004800:Enzymes, Immobilized; C033301:bismuth oxide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D001729:Bismuth