[Effect of supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs on p38 MAPK signaling pathway in kidney of early diabetic rats].
OBJECTIVE
To study the effect of supplementing Qi-nourishing Yin and dispersing blood stasis-dredging collateral herbs on p38 mitogen activated protein kinase (p38 MAPK) signaling pathway in the kidney of early diabetic rats.


METHODS
Dividing SD rats randomly into 6 groups: Simple nephrectomy group, model group, irbesatan group, traditional Chinese medicine (TCM) low dose group, TCM middle dose group and TCM high dose group. Each group of rats was fed with the corresponding dose of medicine. After 6 weeks, detecting 24 h urine protein (UPro) level, renal function, p38 MAPK mRNA and p-p38 MAPK protein level.


RESULTS
UPro levels of irbesatan group, TCM low group and TCM middle dose group decreased significantly (P < 0.05) , compared with that of the model group. Renal function of the treated groups was improved greatly and their p38 MAPK mRNA and p-p38MAPK protein levels decreased significantly (P < 0.05), compared with those of the model group.


CONCLUSIONS
Supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs could treat DN rats effectively by inhibiting the expression of p38 MAPK signaling pathway.
Zhao
Wenhong
W
Urology Department, Hospital of Chinese Medicine of Hebei Medical University, Shijiazhuang 050011, China. zwhhrc@163.com
Chen
Zhiqiang
Z
Zhang
Jianghua
J
Sun
Yufeng
Y
Wang
Yuehua
Y
Wang
Huiqing
H
D000818:Animals; D019659:Asteraceae / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003937:Diagnosis, Differential; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D007677:Kidney Function Tests / Q000379:methods*; D007684:Kidney Tubules / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D008516:Medicine, Chinese Traditional / Q000379:methods; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D014517:Ureteral Obstruction / Q000139:chemically induced; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D048051:p38 Mitogen-Activated Protein Kinases