Determination of organic acids in Vaccinium berry standard reference materials.
Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.
Phillips
Melissa M
MM
Analytical Chemistry Division, Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. melissa.phillips@nist.gov
Case
Ryan J
RJ
Rimmer
Catherine A
CA
Sander
Lane C
LC
Sharpless
Katherine E
KE
Wise
Stephen A
SA
Yen
James H
JH
D000143:Acids / Q000032:analysis* / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D019587:Dietary Supplements / Q000032:analysis* / Q000592:standards*; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D013058:Mass Spectrometry; D012015:Reference Standards; D029796:Vaccinium / Q000737:chemistry*
D000143:Acids