Fresh frozen plasma increases adhesion molecule expression on human pulmonary endothelial cells.
BACKGROUND
Current blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP).


METHODS
Human pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry.


RESULTS
In both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS.


CONCLUSIONS
FFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion.
Letourneau
Phillip A
PA
Department of Surgery, University of Texas Medical School at Houston, Houston, Texas 77030, USA. phillip.a.letourneau@uth.tmc.edu
Pati
Shibani
S
Gerber
Michael H
MH
Jimenez
Fernando
F
Holcomb
John B
JB
D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D019040:E-Selectin / Q000032:analysis; D042783:Endothelial Cells / Q000737:chemistry*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000737:chemistry*; D019007:P-Selectin / Q000032:analysis; D010949:Plasma / Q000502:physiology*; D012128:Respiratory Distress Syndrome / Q000209:etiology; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D008070:Lipopolysaccharides; D019007:P-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1