Histopathological analysis of initial cellular response in TLR-2 deficient mice experimentally infected by Leishmania (L.) amazonensis.
Tegumentary leishmaniasis is an important public health problem in several countries. The capacity of the Leishmania species, at the initial moments of the infection, to invade and survive inside the host cells involves the interaction of surface molecules that are crucial in determining the evolution of the disease. Using C57BL/6 wild-type and TLR-2(-/-) mice infected with L. (L.) amazonensis, we demonstrated that TLR-2(-/-) mice presented eosinophilic granuloma in the ear dermis, different from C57BL/6 wild-type mice that presented a cellular profile characterized mainly by mononuclear cell infiltrates, besides neutrophils and eosinophils, during the two first week of infection. When the parasite load was evaluated, we found that the absence of TLR-2 lead to a significant reduction of the infection in deficient mice, when compared with C57BL/6 mice which were more susceptible to the infection. Using TLR-2 deficient mice, it was possible to show that the absence of this receptor determined the reduction of the parasite load and the recruitment of inflammatory cells during the two first weeks after L. (L.) amazonensis infection.
Guerra
Camila Silva
CS
Laboratory of Structural Biology, Oswaldo Cruz Institute, FIOCRUZ - Rio de Janeiro/RJ-Brazil.
Silva
Roger Magno Macedo
RM
Carvalho
Luís Otávio Pereira
LO
Calabrese
Kátia da Silva
KS
Bozza
Patrícia Torres
PT
Côrte-Real
Suzana
S
D000818:Animals; D020405:Dermis / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D018450:Disease Progression; D005260:Female; D007891:Leishmania / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology*; D008264:Macrophages / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009504:Neutrophils / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000235:genetics* / Q000276:immunology
C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2