Zeeman laser scanning confocal microscope and its ability on reduction of specimen-induced spherical aberration.
The spherical aberration induced by refractive-index mismatch results in the degradation on the quality of sectioning images in conventional confocal laser scanning microscope (CLSM). In this research, we have derived the theory of image formation in a Zeeman laser scanning confocal microscope (ZLSCM) and conducted experiments in order to verify the ability of reducing spherical aberration in ZLSCM. A Zeeman laser is used as the light source and produces the linearly polarized photon-pairs (LPPP) laser beam. With the features of common-path propagation of LPPP and optical heterodyne detection, ZLSCM shows the ability of reducing the specimen-induced spherical aberration and improving the axial resolution simultaneously.
Wu
Jheng-Syong
JS
Department of Optics and Photonics, National Central University, Jhongli 320, Taiwan.
Chou
Chien
C
Chang
Chi-Hui
CH
Yu
Li-Ping
LP
Chou
Li-Dek
LD
Chang
Hsiu-Fong
HF
Yau
Hon-Fai
HF
Lee
Cheng-Chung
CC