Influence of physicochemical and operational parameters on Nitrobacter and Nitrospira communities in an aerobic activated sludge bioreactor.
To understand how to optimize performance of a partially nitrifying plant, the dynamics of Nitrospira and Nitrobacter abundance were studied over a 1 year period using quantitative polymerase chain reaction (qPCR) and their relative contributions to nitrite oxidation assessed including the affects of temperature and dissolved oxygen (DO). Correlation coefficients linking shifts in the community composition of nitrite-oxidizing bacteria (NOB) to operational or environmental variables indicated Nitrospira was significantly and negatively correlated to nitrite concentrations (r = -0.45, P < 0.01) and DO (r = -0.46, P < 0.01), while temperature showed a strong positive correlation (r = 0.59, P < 0.0001). However, the Nitrobacter portion of the total NOB populations showed a positive correlations with DO (r = 0.38, P < 0.01) and hydraulic retention time (HRT) (r = 0.33, P < 0.05), as well as being negatively correlated with temperature (r = -0.49, P < 0.001) suggesting specific niche adaptations within the NOB community. Nitrospira was dominant being better adapted to the low DO and shorter sludge retention times (SRT) of this plant, while Nitrobacter increased in abundance during the winter months, when temperatures were lower and DO concentrations higher. Principal component analysis (PCA) results supported these findings by the close proximity of Nitrospira and temperature biplots of PC1 and PC2 as well as grouping Nitrobacter, NO(2)(-)-N, HRT, and DO in the loadings together. The clustering of samples from specific dates also exhibited a strong seasonality.
Huang
Zhonghua
Z
Department of Environmental Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. hzhlqfox@mail.njust.edu.cn
Gedalanga
Phillip B
PB
Asvapathanagul
Pitiporn
P
Olson
Betty H
BH
D000332:Aerobiosis; D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D017753:Ecosystem; D006863:Hydrogen-Ion Concentration; D009573:Nitrites / Q000378:metabolism; D009576:Nitrobacter / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D013696:Temperature
D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D012722:Sewage