Efficient transfection of sendai virus vector to mouse pancreatic stem cells in the floating state.
Sendai virus (SeV) vectors can efficiently introduce foreign genes without toxicity into various organs and are expected to be clinically applicable. We previously compared the transfectional efficiency of SeV and adenovirus (AdV) vectors by assessing the transfer of the green fluorescent protein (GFP) gene to pancreatic stem cells. Although the gene transfer efficiency was similar between these vectors, SeV vector had a lower toxicity in comparison to the AdV vector. In this study, we assessed the gene transfer efficiency of SeV vector in the floating state to pancreatic stem cells. The efficiency of gene transfer was much higher at all time points and at all concentrations in the floating state versus in the adhesion state. In addition, the pancreatic stem cells transfected with SeV in the floating state maintained their differentiation ability. These data suggest that SeV transfection to pancreatic stem cells in the floating state may be useful in gene transfer technology.
Oishi
Koichi
K
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Noguchi
Hirofumi
H
Yukawa
Hiroshi
H
Inoue
Makoto
M
Miyamoto
Yoshitaka
Y
Iwata
Hisashi
H
Hasegawa
Mamoru
M
Hayashi
Shuji
S
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death; D002454:Cell Differentiation; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007328:Insulin / Q000096:biosynthesis; D051379:Mice; D010179:Pancreas / Q000166:cytology*; D029082:Sendai virus / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D007328:Insulin; D049452:Green Fluorescent Proteins