title: Amounts and proportion of administered pyrene dose excreted as urinary 1-hydroxypyrene after dietary exposure to polycyclic aromatic hydrocarbons.
abstract: Although urinary 1-hydroxypyrene (1-OHP) is the most relevant parameter for assessing exposure to polycyclic aromatic hydrocarbons, the inability to further elucidate the intra- and inter-individual variability, specificity and kinetics makes it difficult to enhance its value as an exposure predictor. Therefore, this human control study examined the excretion kinetics of urinary 1-OHP after consuming barbecued meat. Two feeding experiments were conducted, with doses of 15 and 30 g of barbecued meat per kg of body weight for experiments 1 and 2, respectively. All voided urine was collected for 7 days and analyzed for 1-OHP. In both experiments, the amounts of urinary 1-OHP excreted was significantly increased (P < 0.05) at 12 h post exposure but not at 12-24 h post exposure. Mean percentages of administered pyrene doses excreted as urinary 1-OHP at 0-12 h and 12-24 h post exposure were 3.80 and 0.61% in experiment 1 and 1.66 and 0.38% in experiment 2. Excretion ratio was inversely related to dose. A pattern of diurnal fluctuation (P < 0.05) in 1-OHP excretions was also identified. That is, 1-OHP excretions were smaller in the first half of the day (~0:00-12:00) than in the last half of the day (~12:00-24:00). This study demonstrated that, even at large dietary doses, most of the total urinary excretion of 1-OHP occurs within 12 h. Thus, subjects of occupational or environmental studies need only recall their diets for the current or previous day to diminish the influence from dietary pyrene.
authors.affiliation: Department of Safety, Health and Environmental Engineering, Hungkuang University, Shalu 433, Taiwan. yc@sunrise.hk.edu.tw
authors.forename: Yeh-Chung
authors.initials: YC
authors.lastname: Chien
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Yeh
mesh_terms: D000328:Adult; D001272:Atmosphere / Q000737:chemistry; D015415:Biomarkers / Q000652:urine; D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000652:urine*; D005260:Female; D005449:Fluorenes / Q000032:analysis; D006801:Humans; D008297:Male; D008460:Meat / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000652:urine; D011721:Pyrenes / Q000032:analysis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C006703:benzo(b)fluoranthene; C033146:1-hydroxypyrene