A comparison of bone generation capability in rabbits using tooth ash and plaster of Paris with platelet-rich plasma or fibrin sealant.
OBJECTIVE
Increased attention has been focused on determining the most efficacious materials for generalized bone grafts. This article presents the results of a histomorphometric analysis of bone healing in the calvaria of rabbits. The study compared the use of a tooth ash and plaster of Paris mixture alone, in association with platelet-rich plasma (PRP), and in association with fibrin sealant.


METHODS
Twelve rabbits were divided into control (group 1) and experimental groups (groups 2, 3, and 4). Group 1 was maintained as an unfilled control, and tooth ash and plaster of Paris were used in group 2, tooth ash and plaster of Paris with PRP were used in group 3, and tooth ash and plaster of Paris with fibrin sealant (Tissucol Duo Quick) were used in group 4. One-half of the animals were killed after 4 weeks, and the rest were killed after 8 weeks. Bone samples were taken from the defect areas, and newly formed bone was analyzed histomorphometrically.


RESULTS
The rate of new bone formation in groups 2, 3, and 4 was significantly higher than the rate in the control group. The rate of new bone formation in groups 3 and 4 was higher than the rate in group 2, but the difference was not statistically significant.


CONCLUSIONS
The concomitant use of PRP or fibrin sealant with tooth ash and plaster of Paris graft materials may have a positive effect on bone healing.
Kim
Bong-Kyun
BK
Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Korea.
Kim
Su-Gwan
SG
Kim
Seo-Yoon
SY
Lim
Sung-Chul
SC
Kim
Young-Kyun
YK
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002133:Calcium Sulfate / Q000737:chemistry / Q000494:pharmacology; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology; D020007:Implants, Experimental; D008297:Male; D008903:Minerals / Q000737:chemistry / Q000494:pharmacology*; D016348:Osseointegration / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000187:drug effects / Q000601:surgery; D014070:Tooth / Q000737:chemistry
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D045424:Complex Mixtures; D004338:Drug Combinations; D015718:Fibrin Tissue Adhesive; D006882:Hydroxyapatites; D008903:Minerals; C485817:beta-tricalcium phosphate; D002133:Calcium Sulfate