title: [Technical optimization for extracting hypotensive active peptides from Agrocybe aegerita].
abstract: OBJECTIVE
  To optimize the techniques for extracting hypotensive active peptides from Agrocybe aegerita.
  
  
  METHODS
  The effects of the liquid/solid ratio, extraction time and temperature, pH value of the initial liquid on the extraction percentage (EP) of the hypotensive active peptides were investigated, and inhibition percentage (IP) of the extracts on angiotensin I-converting enzyme was determined.
  
  
  RESULTS
  Optimal extraction of the hypotensive active peptides from Agrocybe aegerita was achieved with the liquid/solid ratio of 40:1, extraction time of 3 h, extraction temperature at 30 degrees Celsius;, and pH=8 of the initial liquid. The EP of the hypotensive active peptides from Agrocybe aegerita could reach 87.7% with IP of the extracts on angiotensin I-converting enzyme of 54.0%.
  
  
  CONCLUSIONS
  This method is simple and efficient for extracting hypotensive active peptides from Agrocybe aegerita.
authors.affiliation: College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
authors.forename: Shan-gang
authors.initials: SG
authors.lastname: Wu
authors.forename: Hong-na
authors.initials: HN
authors.lastname: Sun
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Sun
authors.forename: Dan-kui
authors.initials: DK
authors.lastname: Liao
mesh_terms: D055362:Agrocybe / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004044:Dietary Proteins / Q000302:isolation & purification / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000959:Antihypertensive Agents; D004044:Dietary Proteins; D010455:Peptides