title: Rapid enumeration of respiratory active mycobacteria with fluorescent double staining.
abstract: A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ichijo
authors.forename: Yoko
authors.initials: Y
authors.lastname: Izumi
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Masao
authors.initials: M
authors.lastname: Nasu
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D009161:Mycobacterium / Q000737:chemistry* / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D010100:Oxygen