Retinoid storage in the egg of reptiles and birds.
Storage of retinal has been confirmed in eggs from a range of anamniotic vertebrates (teleosts and amphibians) and an ascidian, but the retinoid-storage state in eggs of oviparous amniotic vertebrates (reptiles and birds) has yet to be clarified in detail. We studied four reptilian and five avian species and found that retinal was commonly stored in their egg yolk. Furthermore, retinal was the major retinoid in reptilian eggs, with only low levels of retinol, whereas significant amounts of retinol as well as retinal were stored in avian eggs. In both reptilian and avian eggs, retinal was commonly bound to proteins, which were assumed to be homologous to the proteins that bind retinal in the eggs of anamniotic vertebrates. Despite the common storage state of retinal, retinol would be bound to different proteins. In the reptilian eggs, retinol was found in the yolk-granule fraction, which also contained retinal. However, retinol in avian eggs was found largely in the yolk-plasma fraction, separate from retinal. These results suggest that retinol storage in avian eggs acquired after the divergence of birds from the reptiles, while retinal storage was acquired before the appearance of the vertebrates, and has subsequently been conserved during evolution of oviparous vertebrates.
Irie
Toshiaki
T
Laboratory of Biology, Department of General Education, Hakodate National College of Technology, Tokura-cho, Hakodate, Hokkaido 042-8501, Japan. irie@hakodate-ct.ac.jp
Sugimoto
Tamiko
T
Ueki
Nobuo
N
Senoo
Haruki
H
Seki
Takaharu
T
D000818:Animals; D001717:Birds / Q000378:metabolism* / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004527:Egg Proteins / Q000378:metabolism; D004530:Egg Yolk / Q000378:metabolism; D052287:Oviparity; D010063:Ovum / Q000378:metabolism* / Q000502:physiology; D010802:Phylogeny; D012104:Reptiles / Q000378:metabolism* / Q000502:physiology; D012172:Retinaldehyde / Q000378:metabolism; D012176:Retinoids / Q000737:chemistry / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D004527:Egg Proteins; D012176:Retinoids; D014801:Vitamin A; D012172:Retinaldehyde