title: The reliability of the IL-6, sIL-6R and sgp130 response to a preloaded time trial.
abstract: Interleukin-6 (IL-6) is a cytokine that can mediate numerous biological actions including fatigue. Circulating IL-6 increases during prolonged exercise, and furthermore, the signalling receptors sIL-6R and sgp130 are also increased. The variability of the response of these markers to exercise is unknown; therefore, we examined the changes in these markers to a preloaded time trial bout of running. Nine males performed three identical trials where participants ran at 60% vVO2max for 2 h interspersed with 30 s at 90% vVO2max every 10 min, followed by a 5-km time trial. Blood samples were drawn at baseline, following the 2-h bout, post time trial, 1 h post time trial and the following morning. Results showed that between-subject variability (CVg) was greater than within-subject variation (CVi) for the three markers. IL-6, sIL-6R and sgp130 demonstrated a CVi of 15.3-25.5%, 15.0-17.6% and 6.2-9.4% variation, respectively, across the time points. When the data from the second and third trials were analysed independently, CVi was reduced which is supported by the time trial results for which CVi improve (4.7-2.4%). In conclusion, the results indicate that a large variation in response to exercise can be reduced following a habituation trial.
authors.affiliation: Department of Sport and Exercise Sciences, Northumbria University, City Campus, Newcastle upon Tyne, Tyne and Wear NE1 8ST, UK. Ian.walshe@unn.ac.uk
authors.forename: I
authors.initials: I
authors.lastname: Walshe
authors.forename: P
authors.initials: P
authors.lastname: Robson-Ansley
authors.forename: A
authors.initials: A
authors.lastname: St Clair Gibson
authors.forename: C
authors.initials: C
authors.lastname: Lawrence
authors.forename: K G
authors.initials: KG
authors.lastname: Thompson
authors.forename: L
authors.initials: L
authors.lastname: Ansley
mesh_terms: D000328:Adult; D056352:Athletes; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D050822:Cytokine Receptor gp130 / Q000097:blood*; D005080:Exercise Test / Q000379:methods*; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D015850:Interleukin-6 / Q000097:blood*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology; D011659:Pulmonary Gas Exchange / Q000502:physiology; D019947:Receptors, Interleukin-6 / Q000097:blood*; D012420:Running / Q000502:physiology*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D015850:Interleukin-6; D019947:Receptors, Interleukin-6; D050822:Cytokine Receptor gp130; D019344:Lactic Acid; D002097:C-Reactive Protein; D006854:Hydrocortisone