An ultrahighly sensitive and selective electrochemical DNA sensor via nicking endonuclease assisted current change amplification.
Based on the "nicking endonuclease assisted" detection strategy, a novel electrochemical biosensor for the sequence-specific detection of DNA with ultrahigh sensitivity and selectivity is developed. By employing the above strategy, this DNA biosensor can detect as low as 0.068 fM target DNA and exhibits high discrimination ability even against a single-base mismatch.
Chen
Jinghua
J
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Zhang
Jing
J
Li
Juan
J
Fu
Fengfu
F
Yang
Huang-Hao
HH
Chen
Guonan
G
D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D053904:DNA Breaks, Single-Stranded; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*
D004247:DNA; D004262:DNA Restriction Enzymes