title: Preparation and characterization of ceria-zirconia composite for enrichment and identification of phosphopeptides.
abstract: Ceria-zirconia composites at different molar ratios were synthesized. Several methods were used to characterize these composites, including X-ray photoelectron spectroscopy, surface area and surface acid-base property detection. A one-step method for isolation and identification of phosphopeptides from peptide mixture was created using these ceria-zirconia composites. Using tryptic digest of standard phosphorylated protein, we have shown that these enrichment and dephosphorylation activities are effective. The adsorption capacity and catalytic property of ceria-zirconia composites at different molar ratios and calcinated temperatures were studied. In combination with MALDI-TOF-based peptide mass finger printing technique, we have established a method to utilize the enrichment/dephosphorylation dual properties of these ceria-zirconia composites for the analysis of phosphoprotein in nonfat milk successfully.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, P. R. China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Kuang
authors.initials: K
authors.lastname: Xiao
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D002563:Cerium / Q000737:chemistry*; D010316:Particle Size; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015040:Zirconium / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; D002563:Cerium; C030583:ceric oxide; D015040:Zirconium; C028541:zirconium oxide