Analysis of polymorphisms in genes (AGT, MTHFR, GPIIIa, and GSTP1) associated with hypertension, thrombophilia and oxidative stress in Mestizo and Amerindian populations of México.
Several polymorphisms related to hypertension, thrombophilia, and oxidative stress has been associated with the development of cardiovascular disease. We analyzed the frequency of M235T angiotensinogen (AGT), A222V 5,10 methylenete-trahydrofolate reductase (MTHFR), L33P glycoprotein IIIa (GPIIIa), and I105V glutathione S-transferase P1 (GSTP1)} polymorphisms in 285 individuals belonging to Mexican-Mestizo and five Amerindian population from México, by real time PCR allelic discrimination. Allele and genotype frequencies were compared using chi square tests. All populations followed the Hardy Weinberg equilibrium for assay markers with the exception of the Triki, whose were in Hardy Weinberg dysequilibrium for the glutathione S-transferase P1 polymorphism. Interestingly, according to all the analyzed single nucleotide polymorphisms (SNPs), the Triki population was the most differentiated and homogeneous group of the six populations analyzed. A comparison of our data with those previously published for some Caucasian, Asian and Black populations showed quite significant differences. These differences were remarkable with all the Mexican populations having a lower frequency of the 105V allele of the glutathione S-transferase P1 and reduced occurrence of the 222A allele of the 5,10 methylenetetrahydrofolate reductase. Our results show the genetic diversity among different Mexican populations and with other racial groups.
Juárez-Velázquez
Rocio
R
Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., México.
Canto
Patricia
P
Canto-Cetina
Thelma
T
Rangel-Villalobos
Hector
H
Rosas-Vargas
Haydee
H
Rodríguez
Maricela
M
Canizales-Quinteros
Samuel
S
Velázquez Wong
Ana Claudia
AC
Ordoñez-Razo
Rosa María
RM
Vilchis-Dorantes
Guadalupe
G
Coral-Vázquez
Ramón Mauricio
RM
D000293:Adolescent; D000328:Adult; D000808:Angiotensinogen / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D004247:DNA / Q000097:blood / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D007198:Indians, North American / Q000235:genetics; D039661:Integrin beta3 / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D019851:Thrombophilia / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D044465:White People / Q000235:genetics; D055815:Young Adult
D039661:Integrin beta3; D000808:Angiotensinogen; D004247:DNA; D042965:Methylenetetrahydrofolate Reductase (NADPH2); C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi