title: Pro-inflammatory cytokine and chemokine mRNA blood level in multiple sclerosis is related to treatment response and interferon-beta dose.
abstract: Of 37 multiple sclerosis patients, 19 suboptimal responders were randomized to 375 (n=12) or 250µg (n=7) interferon (IFN)-β-1b. mRNA levels of 23 cytokines, chemokines, and chemokine receptors were quantified by TaqMan low-density array (TLDA) real-time polymerase chain reaction. Better treatment responses or increased IFN-β doses were associated with elevated IL-10 and TGF-β and decreased CXCL10, IL-18, IFN-γ, and TNF-α transcript levels. Adjusting for dose, poor treatment responses resulted in a 4-fold increase in CXCL10 and IFN-γ expression (Mantel-Haenszel RR=3.74, p<0.0001). CXCL10 and IFN-γ mRNA levels were reliable indicators of treatment response. TLDA can be used to tailor IFN-β-1b therapy.
authors.affiliation: University Division of Neurology, Facolta' San Luigi Gonzaga, Universita' di Torino, Ospedale Universitario San Luigi Gonzaga, Regione Gonzole, 10, I-10043 Orbassano, Turin, Italy.
authors.forename: Angele
authors.initials: A
authors.lastname: Cucci
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Barbero
authors.forename: Marinella
authors.initials: M
authors.lastname: Clerico
authors.forename: Bruno
authors.initials: B
authors.lastname: Ferrero
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Versino
authors.forename: Giulia
authors.initials: G
authors.lastname: Contessa
authors.forename: Stefania
authors.initials: S
authors.lastname: Demercanti
authors.forename: Emanuela
authors.initials: E
authors.lastname: Viglietta
authors.forename: Alessandra
authors.initials: A
authors.lastname: Di Liberto
authors.forename: Alessandra Giai
authors.initials: AG
authors.lastname: Vai
authors.forename: Luca
authors.initials: L
authors.lastname: Durelli
mesh_terms: D000328:Adult; D016207:Cytokines / Q000097:blood* / Q000145:classification / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology / Q000627:therapeutic use*; D008137:Longitudinal Studies; D008297:Male; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology*; D012333:RNA, Messenger / Q000097:blood*; D055815:Young Adult
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D016899:Interferon-beta