Analyzing binding data.
Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments.
Motulsky
Harvey J
HJ
GraphPad Software, La Jolla, California, USA.
Neubig
Richard R
RR
D001667:Binding, Competitive; D005453:Fluorescence; D005454:Fluorescence Polarization / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D007700:Kinetics; D008024:Ligands; D016014:Linear Models; D008956:Models, Chemical; D017711:Nonlinear Dynamics; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000737:chemistry; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
D008024:Ligands; D019275:Radiopharmaceuticals; D043562:Receptors, G-Protein-Coupled