title: Survivin mediates self-protection through ROS/cdc25c/CDK1 signaling pathway during tumor cell apoptosis induced by high fluence low-power laser irradiation.
abstract: Survivin, an important member of inhibitor-of-apoptosis (IAP) family, can be up-regulated by various pro-apoptotic stimuli, such as UV, photodynamic therapy (PDT) and cisplatin. High fluence low-power laser irradiation (HF-LPLI) is a newly discovered pro-apoptotic stimulator. The anti-apoptotic mechanism of survivin during HF-LPLI-induced apoptosis is still not investigated. Here, we report that HF-LPLI up-regulates survivin activity through reactive oxygen species (ROS)/cdc25c protein phosphatase (cdc25c)/cyclin-dependent kinase (CDK1) signaling pathway in human lung adenocarcinoma cells (ASTC-a-1). The up-regulation of survivin activity can reduce HF-LPLI-induced apoptosis, while down-regulation of the activity can promote the apoptosis. In addition, activated survivin delays mitochondrial depolarization, cytochrome c release, caspase-9 and Bax activation, all of which are typical pro-apoptotic events of cell apoptosis induced by HF-LPLI. On the basis of the present studies, we conclude that survivin can mediate self-protection during tumor cell apoptosis caused by HF-LPLI.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Jiru
authors.initials: J
authors.lastname: Chu
authors.forename: Shengnan
authors.initials: S
authors.lastname: Wu
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D016203:CDC2 Protein Kinase / Q000378:metabolism; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D003574:Cytochrome c Group / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D028022:Low-Level Light Therapy / Q000379:methods*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects*; D000077022:Survivin; D014162:Transfection; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D020687:cdc25 Phosphatases / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C107489:BIRC5 protein, human; D061945:Caspase Inhibitors; D003574:Cytochrome c Group; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D017382:Reactive Oxygen Species; D000077022:Survivin; D051028:bcl-2-Associated X Protein; D016203:CDC2 Protein Kinase; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases; D020169:Caspases