[Study on Tie2 mRNA level in peripheral blood associated with the severity of sepsis].
OBJECTIVE
To investigate the possibility that the level of Tie2 mRNA in peripheral blood could reflect the severity of sepsis.


METHODS
Trauma patients in intensive care unit (ICU) were recruited, and they were divided into sepsis group (n=13) and non-sepsis group (n=19). The severity of disease was evaluated with acute physiology and chronic health evaluation II (APACHEII) score on the day of ICU admission. Blood of patients was sampled on day 1, 3, 7 after ICU admission to determination of the white blood cell (WBC) count, contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (Cr), mRNA levels of Tie2 in blood measured by quantitative real-time polymerase chain reaction (PCR), and the contents of plasma von Willebrand factor (vWF) with enzyme linked immunosorbent assay (ELISA).


RESULTS
No significant difference in contents of ALT, AST and plasma vWF was found between sepsis group and non-sepsis group [ALT (U/L): 53.30 (199.58) vs. 80.65 (202.62), AST (U/L): 316.53 (49.90) vs. 66.10 (285.03), vWF: (272.47+/-114.61)% vs. (246.66+/-128.77)%, all P>0.05]. The number of WBC [x10(9)/L, 18.26 (21.82) vs. 10.11 (4.72)], the contents of BUN [mmol/L, 20.70 (11.20) vs. 7.70 (5.45)] and Cr [micromol/L: 252.00 (364.55) vs. 68.00(23.20)], and the circulating mRNA levels of Tie2 (1.86+/-0.67 vs. 0.91+/-0.42) in sepsis group were higher than those in the non-sepsis group (all P<0.01). The Tie2 mRNA level in peripheral blood of each patient was positively correlated with APACHEII score (r=0.532, P<0.01). The linear regression equation was Y=12.66+4.922 X (R2=0.283). Besides, there was a significant correlation between the amount of Tie2 mRNA and plasma levels of vWF (r=0.334, P<0.05). The linear regression equation was Y=180.932+57.93 X (R2=0.112).


CONCLUSIONS
The level of Tie2 mRNA in peripheral blood could reflect the damage of endothelial cell and severity of sepsis.
Yang
Jing
J
State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Huang
Jian
J
Yang
Wen-qiong
WQ
Zhou
Jian
J
Jiang
Dong-po
DP
Zhang
Yao-zong
YZ
Chen
Lin
L
D018806:APACHE; D000328:Adult; D042783:Endothelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood; D042787:Receptor, TIE-2 / Q000097:blood*; D018805:Sepsis / Q000097:blood*; D055815:Young Adult
D012333:RNA, Messenger; D042787:Receptor, TIE-2