Expression of transient receptor potential channel vanilloid (TRPV) 1&#x2013;4, melastin (TRPM) 5 and 8, and ankyrin (TRPA1) in the normal and methimazole-treated mouse olfactory epithelium.
CONCLUSIONS
It is suggested that TRPV1, 2, 3, and 4, TRPM5 and 8, and TRPA1 may play several roles in the olfactory epithelium (OE), contributing to olfactory chemosensation, olfactory adaptation, olfactory–trigeminal interaction, and OE fluid homeostasis. In patients with olfactory disturbance, TRPV1 and TRPM8 may be closely related to a high rate of recognition of curry and menthol odors, while TRPV2 may also play a crucial role in the regeneration of olfactory receptor neurons.


OBJECTIVE
Expression of TRPV1–4, TRPM5 and 8, and TRPA1 in the normal and methimazole-treated mouse OE was analyzed.


METHODS
The localization of TRPV1–4, TRPM5 and 8, and TRPA1 in the OE of normal and methimazole-treated CBA/J mice was investigated by immunohistochemistry.


RESULTS
Normal OE showed a positive immunofluorescent reaction to TRPV1–4, TRPM5 and 8, and TRPA1. In lamina propria, the nerve fibers displayed TRPV 1, 2, and 3, TRPM8 and TRPA1. In the pathological condition, the expression of TRPV3, TRPV4, TRPM5, and TRPA1 was markedly reduced and took a long time to recover. In contrast, expression of TRPM8 was scarcely affected, even in the pathological condition, while TRPV1 and TRPV2 showed early recovery following methimazole treatment.
Nakashimo
Yousuke
Y
Department of Otolaryngology, Hiroshima University, Japan. yosabnm1@hiroshima-u.ac.jp
Takumida
Masaya
M
Fukuiri
Takashi
T
Anniko
Matti
M
Hirakawa
Katsuhiro
K
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008713:Methimazole / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D009831:Olfactory Mucosa / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D012016:Reference Values; D000074025:TRPA1 Cation Channel; D050053:TRPM Cation Channels / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism*; D014018:Tissue Distribution; D050051:Transient Receptor Potential Channels / Q000378:metabolism*
D015220:Calcium Channels; D000074025:TRPA1 Cation Channel; D050053:TRPM Cation Channels; C492297:TRPM8 protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D050051:Transient Receptor Potential Channels; C475685:Trpa1 protein, mouse; C498617:Trpm5 protein, mouse; C485717:Trpv2 protein, mouse; C486760:Trpv3 protein, mouse; C486576:Trpv4 protein, mouse; D008713:Methimazole