title: Epstein-Barr virus encoded EBNA-3 binds to vitamin D receptor and blocks activation of its target genes.
abstract: Epstein-Barr virus (EBV) is a human gamma herpes virus that infects B cells and induces their transformation into immortalized lymphoblasts that can grow as cell lines (LCLs) in vitro. EBNA-3 is a member of the EBNA-3-protein family that can regulate transcription of cellular and viral genes. The identification of EBNA-3 cellular partners and a study of its influence on cellular pathways are important for understanding the transforming action of the virus. In this work, we have identified the vitamin D receptor (VDR) protein as a binding partner of EBNA-3. We found that EBNA3 blocks the activation of VDR-dependent genes and protects LCLs against vitamin-D3-induced growth arrest and/or apoptosis. The presented data shed some light on the anti-apoptotic EBV program and the role of the EBNA-3-VDR interaction in the viral strategy.
authors.affiliation: Department of Microbiology, Tumor, and Cell Biology (MTC), Karolinska Institute, 171 77, Stockholm, Sweden.
authors.forename: Surya Pavan
authors.initials: SP
authors.lastname: Yenamandra
authors.forename: Ulf
authors.initials: U
authors.lastname: Hellman
authors.forename: Bettina
authors.initials: B
authors.lastname: Kempkes
authors.forename: Suhas Deoram
authors.initials: SD
authors.lastname: Darekar
authors.forename: Sabine
authors.initials: S
authors.lastname: Petermann
authors.forename: Tom
authors.initials: T
authors.lastname: Sculley
authors.forename: George
authors.initials: G
authors.lastname: Klein
authors.forename: Elena
authors.initials: E
authors.lastname: Kashuba
mesh_terms: D000818:Animals; D002112:Calcifediol / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D013058:Mass Spectrometry; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism*
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens; D018167:Receptors, Calcitriol; D002112:Calcifediol