title: Dissecting the cell surface.
abstract: The bacterial cell surface is an important structure as it mediates interactions with the external environment. In the case of pathogens like Clostridium difficile, the cell wall and its components also have to mediate interactions with the host cells and their products. In this chapter we discuss the various methods used for dissecting the cell surface and the biochemical and immunological procedures that are commonly used to analyse the properties of the proteins within the cell wall. A major consideration is the S-layer which in C. difficile shows considerable variation in sequence and between strains, a property which is also reflected in its antigenic properties.
authors.affiliation: Centre for Molecular Microbiology and Infection, Imperial College London, London, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Fagan
authors.forename: Neil
authors.initials: N
authors.lastname: Fairweather
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002473:Cell Wall / Q000378:metabolism*; D016360:Clostridioides difficile / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins