title: Purification and characterization of lipopolysaccharides.
abstract: Lipopolysaccharides are the major components on the surface of most Gram-negative bacteria, and recognized by immune cells as a pathogen-associated molecule. They can cause severe diseases like sepsis and therefore known as endotoxins. Lipopolysaccharide consists of lipid A, core oligosaccharide and O-antigen repeats. Lipid A is responsible for the major bioactivity of endotoxin. Because of their specific structure and amphipathic property, purification and analysis of lipopolysaccharides are difficult. In this chapter, we summarize the available approaches for extraction, purification and analysis of lipopolysaccharides.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China. xwang65@gmail.com
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
authors.forename: Chan
authors.initials: C
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D004492:Edetic Acid / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods; D015151:Immunoblotting; D008050:Lipid A / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D008050:Lipid A; D008070:Lipopolysaccharides; D012997:Solvents; D014867:Water; D004492:Edetic Acid