title: Mitochondrial matrix delivery using MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes.
abstract: Mitochondria are the principal producers of energy in cells of higher organisms. It was recently reported that mutations and defects in mitochondrial DNA (mtDNA) are associated with various mitochondrial diseases including a variety of neurodegenerative and neuromuscular diseases. Therefore, an effective mitochondrial gene therapy and diagnosis would be expected to have great medical benefits. To achieve this, therapeutic agents need to be delivered into the innermost mitochondrial space (mitochondrial matrix), which contains the mtDNA pool. We previously reported on the development of MITO-Porter, a liposome-based carrier that introduces macromolecular cargos into mitochondria via membrane fusion. In this study, we provide a demonstration of mitochondrial matrix delivery and the visualization of mitochondrial genes (mtDNA) in living cells using the MITO-Porter. We first prepared MITO-Porter containing encapsulated propidium iodide (PI), a fluorescent dye used to stain nucleic acids to detect mtDNA. We then confirmed the emission of red-fluorescence from PI by conjugation with mtDNA, when the carriers were incubated in the presence of isolated rat liver mitochondria. Finally, intracellular observation by confocal laser scanning microscopy clearly verified that the MITO-Porter delivered PI to the mitochondrial matrix.
authors.affiliation: Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yasuzaki
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000032:analysis / Q000378:metabolism*; D008561:Membrane Fusion; D018613:Microscopy, Confocal; D008930:Mitochondria, Liver / Q000737:chemistry / Q000378:metabolism*; D028361:Mitochondrial Diseases / Q000628:therapy; D051336:Mitochondrial Membranes / Q000737:chemistry / Q000378:metabolism*; D011419:Propidium / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014161:Transduction, Genetic
chemical_list: D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes; D008081:Liposomes; D011419:Propidium