title: Distinct macrophage subpopulations regulate viral encephalitis but not viral clearance in the CNS.
abstract: Intranasal application of vesicular stomatitis virus (VSV) induces acute encephalitis characterized by a pronounced myeloid and T cell infiltrate. The role of distinct phagocytic populations on VSV encephalitis was therefore examined in this study. Ablation of peripheral macrophages did not impair VSV encephalitis or viral clearance from the brain, whereas, depletion of splenic marginal dendritic cells impaired this response and enhanced morbidity/mortality. Selective depletion of brain perivascular macrophages also suppressed this response without altering viral clearance. Thus, two anatomically distinct phagocytic populations regulate VSV encephalitis in a non-redundant fashion although neither population is essential for viral clearance in the CNS.
authors.affiliation: Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA. steelcd@evms.edu
authors.forename: Christina D
authors.initials: CD
authors.lastname: Steel
authors.forename: Woong-Ki
authors.initials: WK
authors.lastname: Kim
authors.forename: Larry D
authors.initials: LD
authors.lastname: Sanford
authors.forename: Laurie L
authors.initials: LL
authors.lastname: Wellman
authors.forename: Sandra
authors.initials: S
authors.lastname: Burnett
authors.forename: Nico
authors.initials: N
authors.lastname: Van Rooijen
authors.forename: Richard P
authors.initials: RP
authors.lastname: Ciavarra
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D050071:Bone Density Conservation Agents / Q000009:adverse effects / Q000819:agonists; D002490:Central Nervous System / Q000473:pathology*; D004002:Clodronic Acid / Q000009:adverse effects; D004195:Disease Models, Animal; D018792:Encephalitis, Viral / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D008264:Macrophages / Q000145:classification* / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D010537:Peritoneum / Q000187:drug effects / Q000473:pathology; D016559:Tacrolimus / Q000031:analogs & derivatives / Q000494:pharmacology; D018116:Vesiculovirus / Q000472:pathogenicity
chemical_list: C463061:AP20187; D050071:Bone Density Conservation Agents; C485184:enhanced green fluorescent protein; D004002:Clodronic Acid; D049452:Green Fluorescent Proteins; D016559:Tacrolimus