Dynamic deformation of red blood cell in dual-trap optical tweezers.
Three-dimensional dynamic deformation of a red blood cell in a dual-trap optical tweezers is computed with the elastic membrane theory and is compared with the experimental results. When a soft particle is trapped by a laser beam, the particle is deformed depending on the radiation stress distribution whereas the stress distribution on the particle in turn depends on the deformation of its morphological shape. We compute the stress re-distribution on the deformed cell and its subsequent deformations recursively until a final equilibrium state solution is achieved. The experiment is done with the red blood cells in suspension swollen to spherical shape. The cell membrane elasticity coefficient is obtained by fitting the theoretical prediction with the experimental data. This approach allows us to evaluate up to 20% deformation of cell's shape.
Rancourt-Grenier
Sebastien
S
Center for Optics, Photonics and Lasers, Laval University, Quebec, G1K 7P4, Canada.
Wei
Ming-Tzo
MT
Bai
Jar-Jin
JJ
Chiou
Arthur
A
Bareil
Paul P
PP
Duval
Pierre-Luc
PL
Sheng
Yunlong
Y
D002478:Cells, Cultured; D003198:Computer Simulation; D055119:Elastic Modulus / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D006244:Hardness / Q000502:physiology; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008955:Models, Cardiovascular; D052898:Optical Tweezers; D013314:Stress, Mechanical