Insulin- and leptin-regulated fatty acid uptake plays a key causal role in hepatic steatosis in mice with intact leptin signaling but not in ob/ob or db/db mice.
Hepatic steatosis results from several processes. To assess their relative roles, hepatocellular long-chain fatty acid (LCFA) uptake was assayed in hepatocytes from C57BL/6J control mice, mice with steatosis from a high-fat diet (HFD) or 10%, 14%, or 18% ethanol (EtOH) in drinking water [functioning leptin-signaling groups (FLSGs)], and ob/ob and db/db mice. V(max) for uptake was increased vs. controls (P < 0.001) and correlated significantly with liver weight and triglycerides (TGs) in all FLSG mice but was minimally or not increased in ob/ob and db/db mice, in which liver weights and TGs greatly exceeded projections from regressions in FLSG animals. Coefficients of determination (R(2)) for these FLSG regressions suggest that increased LCFA uptake accounts for ∼80% of the increase in hepatic TGs within these groups, but increased lipogenic gene expression data suggest that enhanced LCFA synthesis is the major contributor in ob/ob and db/db. Got2, Cd36, Slc27a2, and Slc27a5 gene expression ratios were significantly upregulated in the EtOH groups, correlating with sterol regulatory element binding protein 1c (SREBP1c) and V(max), but only Cd36 expression was increased in HFD, ob/ob, and db/db mice. Comparison of V(max) with serum insulin and leptin suggests that both hormones contribute to upregulation of uptake in the FLSG animals. Thus, increased LCFA uptake, reflecting SREBP1c-mediated upregulation of four distinct transporters, is the dominant cause of steatosis in EtOH-fed mice. In ob/ob and db/db mice, increased LCFA synthesis appears more important. In FLSG animals, insulin upregulates hepatocellular LCFA uptake. Leptin appears to upregulate LCFA uptake or to be essential for full expression of upregulation by insulin.
Ge
Fengxia
F
Department of Medicine, Columbia University Medical Center, New York, New York, USA.
Zhou
Shengli
S
Hu
Chunguang
C
Lobdell
Harrison
H
Berk
Paul D
PD
D000375:Aging; D000818:Animals; D001835:Body Weight; D002149:Energy Intake; D000431:Ethanol; D050612:Fatty Acid Transport Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007328:Insulin / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D019301:Oleic Acid / Q000378:metabolism; D009929:Organ Size; D011897:Random Allocation; D054411:Receptors, Leptin / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D007328:Insulin; D020738:Leptin; D008055:Lipids; D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D019301:Oleic Acid; D000431:Ethanol