title: Identification of genes controlled by LMX1B in E13.5 mouse limbs.
abstract: During limb development, the dorsal limb mesenchyme expression of the transcription factor LMX1B is required for dorsoventral limb patterning. In mice, Lmx1b mutations result in the mirror-image duplication of ventral limb structures and loss of dorsal limb structures. Heterozygous LMX1B mutations in humans cause the Nail-Patella Syndrome characterized by limb, kidney, and eye developmental defects. We used DNA microarrays to compare the mRNAs in E13.5 mouse Lmx1b mutant and wild-type limbs. We report 14 genes that require Lmx1b for their normal expression in the dorsal limb or the restriction of their expression to the ventral limb.
authors.affiliation: Neural Circuit Development Laboratory, Institut de recherches cliniques de Montréal (IRCM), 110 avenue des Pins Ouest, Montréal , QC, Canada, H2W 1R7.
authors.forename: Wendy X W
authors.initials: WX
authors.lastname: Gu
authors.forename: Artur
authors.initials: A
authors.lastname: Kania
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D005121:Extremities / Q000196:embryology / Q000254:growth & development*; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D060850:LIM-Homeodomain Proteins; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D018398:Homeodomain Proteins; C112149:LIM homeobox transcription factor 1 beta; D060850:LIM-Homeodomain Proteins; D012333:RNA, Messenger; D014157:Transcription Factors