Regulation of suppressing and activating effects of mesenchymal stem cells on the encephalitogenic potential of MBP68-86-specific lymphocytes.
Mesenchymal stem cells (MSCs) possess immunosuppressive potential and are proposed as a tool for cell therapy, however under certain circumstance they could change to become immunostimulating. It is therefore of great importance to better understand the MSCs' immune plasticity. Here we used different doses of MSCs to co-culture with MBP(68-86)-specific T cells and found that MSCs exerted a suppressive effect on the encephalitogenic ability of MBP(68-86)-specific T cells at high MSC density (MSC/effector ratio ≥ 1:10). Whereas at lower MSC/effector ratios (≤ 1:50), MSCs shifted towards stimulatory activity. Thus the limited amounts of MSCs for application should be considered.
Sun
Bo
B
Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin, Heilongjiang, China.
Zhang
Xinyu
X
Wang
Guangyou
G
Kong
Qingfei
Q
Mu
Lili
L
Wang
Jinghua
J
Zhang
Shujuan
S
Liu
Yumei
Y
Li
Rui
R
Liu
Lei
L
Tian
Ye
Y
An
Yi
Y
Li
Hulun
H
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D004676:Myelin Basic Protein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
D016207:Cytokines; D004676:Myelin Basic Protein; D010446:Peptide Fragments