[HPLC determination of chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas].
To determinate the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas. The HPLC separation was performed on a Inertsil ODS-sp column (4.6 mm x 150 mm, 5 microm). A mixture of acetonitrile and 0.2% phosphoric acid solution (35:65) as the mobile phase the column. Temperature was set in 30 degrees C. The flow rate was 1.0 mL x min(-1), and the wave length of the detector is 203 nm. The content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from Meixian, Shaanxi is the highest and the lowest is from Enshi, Hubei. There have most differerence among the content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas.
Song
Xiaomei
X
Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
Li
Lin
L
Yang
Guangming
G
Cai
Baochang
B
D002851:Chromatography, High Pressure Liquid; D016014:Linear Models; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D005894:Panax / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification
D012503:Saponins; C098580:chikusetsu saponin IVa; D009828:Oleanolic Acid