Impact of 2 doses of clorgyline on the rat preimplantation embryo development and the monoamine levels in urine.
We have evaluated the impact of chronic administration of clorgyline, a potent monoamine oxidase A inhibitor and a former antidepressant, on the preimplantation embryo development in Wistar rats. Females were injected intraperitoneally daily for 30 days with saline (control animals), or with a low-dose clorgyline (LDC, 0.1 mg/kg per d) or with a high-dose clorgyline (HDC, 1 mg/kg per d). Embryos were isolated on day 5 of pregnancy and urine was collected by puncture of the urinary bladder. The number of embryos per female did not differ between experimental groups and control, but we have recorded a decreased number of embryos in HDC group compared to LDC (P < .05). We have found that LDC significantly reduced the presence of healthy embryos and increased the presence of the degenerated embryos (P < .001). The administration of the LDC resulted in the lowest cell number in blastocysts. We have observed significantly increased serotonin levels in HDC group compared to both control (P < .05) and LDC animals (P < .01). Norepinephrine (NE) levels in both experimental groups were significantly elevated compared to controls. Dopamine levels did not differ between groups (P > .05). We speculate that lesser negative effect of HDC compared to LDC on the preimplantation embryo development could be the consequence of the lower NE levels and/or elevated serotonin levels. Potential mechanisms mediating clorgyline-induced impaired preimplantation embryo development are proposed.
Mihalik
Jozef
J
Department of Anatomy, Medical Faculty, P J Šafárik University, Košice, Slovak Republic. jozef.mihalik@upjs.sk
Mašlanková
Jana
J
Špakovská
Tatiana
T
Mareková
Mária
M
Hodorová
Ingrid
I
Kušnír
Jaroslav
J
Rybárová
Silvia
S
Ferenc
Peter
P
Schmidtová
Katarína
K
D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D015306:Biogenic Monoamines / Q000652:urine*; D001755:Blastocyst / Q000187:drug effects / Q000473:pathology; D003010:Clorgyline / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D008996:Monoamine Oxidase Inhibitors / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D000928:Antidepressive Agents; D015306:Biogenic Monoamines; D008996:Monoamine Oxidase Inhibitors; D003010:Clorgyline