Serum SCCA, Cyfra 21-1, EGFR and Cyclin D1 levels in patients with oral squamous cell carcinoma.
OBJECTIVE
To determine the concentrations of SCCA, Cyfra 21-1, EGFR and Cyclin D1 in serum of patients with oral squamous cell carcinoma, and investigate their diagnostic value and their relationship with clinical stage, histological differentiation and lymph node metastasis.


METHODS
Seventy hospitalized patients with oral squamous cell carcinoma and 72 healthy individuals were included in the study. Venous blood was collected from all study participants, in the oral carcinoma patients before tumor resection. One week after surgery, venous blood was collected again from 20 patients. Serum marker levels were determined by enzymelinked immunosorbent assay (ELISA).


RESULTS
The serum SCCA, EGFR and Cyclin D1 concentrations were significantly higher in patients with oral squamous cell carcinoma than in healthy controls, while there was no significant difference in Cyfra 21-1 levels between patients and controls. The serum SCCA concentration decreased after surgery, but there was no significant difference in the serum Cyfra 21-1, EGFR and Cyclin D1 concentrations before and after surgery. Serum SCCA, Cyfra 21-1, EGFR and Cyclin D1 concentrations were not correlated with clinical stage, histological differentiation and lymph node metastasis. When SCCA, EGFR and Cyclin D1 were measured separately, EGFR had the highest diagnostic sensitivity and accuracy and Cyclin D1 had the highest specificity; when any two of the markers were tested in combination, the combined detection of EGFR and Cyclin D1 had the highest sensitivity, specificity and accuracy.


CONCLUSIONS
SCCA, EGFR and Cyclin D1 may prove to be useful tumor markers in oral squamous cell carcinoma. The combined determination of EGFR and Cyclin D1 may be of value in the diagnosis of oral squamous cell carcinoma. Serum SCCA may be used as an adjunct in monitoring treatment response.
Feng
Xiao-yu
XY
Department of Oral and Maxillofacial Surgery, Capital Medical University School of Stomatology, Beijing, China.
Li
Jian-hua
JH
Li
Jin-zhong
JZ
Han
Zheng-xue
ZX
Xing
Ru-dong
RD
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000032:analysis / Q000097:blood*; D066246:ErbB Receptors / Q000032:analysis / Q000097:blood*; D005260:Female; D006801:Humans; D053539:Keratin-19 / Q000032:analysis / Q000097:blood*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D037981:Neck Dissection / Q000534:rehabilitation; D009367:Neoplasm Staging; D011379:Prognosis; D015843:Serpins / Q000032:analysis / Q000097:blood*
D000951:Antigens, Neoplasm; D053539:Keratin-19; D015843:Serpins; C102012:antigen CYFRA21.1; C051072:squamous cell carcinoma-related antigen; D019938:Cyclin D1; C512478:EGFR protein, human; D066246:ErbB Receptors