Cryo-scanning electron microscopy and light microscopy for the study of fungi interactions.
The application of the cryo-scanning electron microscopy and light microscopy for the study of the interactions at different environmental conditions between Penicillium oxalicum and Fusarium verticillioides is described. A dual microculture was developed for the light microscopy analysis of the interaction. The microscope and macroscopic examinations were compared. Analysis of Petri plates revealed that F. verticillioides was a competitor for space and nutrients while P. oxalicum was a mycoparasite under the microscopic observations.
Sempere
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Fondo de Diversidad Fúngica de la Comunidad Valenciana, Unidad Docente de Ecología y Botánica, Departamento de Ecosistemas Agroforestales, Escuela Técnica Superior del Medio Rural y Enología, Universidad Politécnica de Valencia, Avda, Blasco Ibañez, 21. 46010-Valencia, España. frasemfe@yahoo.es.
Santamarina
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MP
D020285:Cryoelectron Microscopy / Q000379:methods*; D005670:Fusarium / Q000302:isolation & purification / Q000378:metabolism*; D056265:Microbial Interactions / Q000502:physiology*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D012275:Oryza / Q000382:microbiology; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D013048:Specimen Handling; D003313:Zea mays / Q000382:microbiology