title: Breast cancer cells attract the migration of adipose tissue-derived stem cells via the PDGF-BB/PDGFR-beta signaling pathway.
abstract: The origin of vascular cells in tumors is unknown, but it is believed that tumors use cells from the host to build new vessels. To determine whether adipose tissue stem cells (ASCs) could be attracted by cancer cells, we performed migration assays in which ASCs were seeded on a transwell migration system top chamber and tumor-conditioned medium was placed in the bottom chamber. Our data showed that a significant number of ASCs migrated toward the tumor-conditioned medium (p<0.0001), and migration of human ASCs significantly (p<0.0001) increased in response to increased concentrations of recombinant PDGF-BB. In addition, neutralizing antibodies to PDGF receptor (PDGFR)-beta decreased migration of ASCs toward a breast cancer-conditioned medium to the level of serum-free control. These data suggest that tumor cell-derived PDGF-BB is an important factor in governing the microenvironment interaction between tumor cells and local tissue-resident stem cells.
authors.affiliation: Department of Molecular Pathology, The University of Texas MD Anderson Cancer Center, Unit 0951, 1515 Holcombe Blvd., Houston, TX 77030, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gehmert
authors.forename: Sanga
authors.initials: S
authors.lastname: Gehmert
authors.forename: Lukas
authors.initials: L
authors.lastname: Prantl
authors.forename: Jody
authors.initials: J
authors.lastname: Vykoukal
authors.forename: Eckhard
authors.initials: E
authors.lastname: Alt
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Song
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000077214:Becaplermin; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000473:pathology*
chemical_list: D057134:Antibodies, Neutralizing; D017077:Culture Media, Conditioned; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020797:Receptor, Platelet-Derived Growth Factor beta