[Study on extraction of anti-diabetes active constituents from Potentilla chinesis].
OBJECTIVE
To study the extraction technology of anti-diabetes active constituents from Potentilla chinesis.


METHODS
Through single factor test, the effects of factors such as concentration of ethanol, temperature, ratio of liquid-solid, extraction time and times of extraction were investigated. Through orthogonal experiment, the optimal conditions of extraction were obtained on the basis of single factor test.


RESULTS
The optimum extraction conditions of Potentilla flavone from P. chinesis were determined as follows: refluxing for 3 times, 1.5 hours each time, and 8-6-6 for the multiple of 60% EtOH and material.


CONCLUSIONS
The extraction process is stable and suitable for industry.
Zhao
Chuan
C
Tianjin Research Center of Basic Medical Science, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China.
Tang
Sheng-an
SA
Duan
Hong-quan
HQ
D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D047309:Flavones / Q000032:analysis / Q000302:isolation & purification*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D031987:Potentilla / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D047309:Flavones; D007004:Hypoglycemic Agents; D000431:Ethanol; C043562:flavone