title: The how, when, and why of the aging signals appearing on the human erythrocyte membrane: an atomic force microscopy study of surface roughness.
abstract: We recently developed an atomic force microscopy-based protocol to use the roughness of the plasma membrane of erythrocytes (red blood cells, RBCs) as a morphological parameter, independently from the cell shape, to investigate the membrane-skeleton integrity in healthy and pathological cells. Here we apply the method to investigate a complex physiological phenomenon, the RBCs aging, that plays a major role in the regulation of the RBCs' turnover. The aging, monitored morphologically and biochemically, has been accelerated and modulated by preventing oxidative stresses as well as the effects of proteases and divalent cations, and by artificially consuming the intracellular adenosine triphosphate. The collected data evidence that the progression of aging causes a drastic decrease of the measured roughness that is diagnostic of a progressive, adenosine triphosphate-dependent alteration of the membrane-skeleton properties. Finally, the degree of reversibility of such effects has been investigated as a function of aging time, enabling the detection of irreversible transformation in the RBCs' structure and metabolism.
authors.affiliation: Istituto di Struttura della Materia - CNR, Rome, Italy. girasole@ism.cnr.it
authors.forename: Marco
authors.initials: M
authors.lastname: Girasole
authors.forename: Giuliano
authors.initials: G
authors.lastname: Pompeo
authors.forename: Antonio
authors.initials: A
authors.lastname: Cricenti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Longo
authors.forename: Giovanna
authors.initials: G
authors.lastname: Boumis
authors.forename: Andrea
authors.initials: A
authors.lastname: Bellelli
authors.forename: Simona
authors.initials: S
authors.lastname: Amiconi
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D013499:Surface Properties