Colored potato extracts induce superoxide dismutase-2 mRNA via ERK1/2 pathway in HepG2 cells.
Rats fed a diet containing Shadow Queen (SQ), an anthocyanin-rich potato cultivar, previously showed an increase in the hepatic superoxide dismutase (SOD)-2 mRNA level. We investigated whether an extract of SQ would directly increase the hepatic SOD-2 mRNA level in HepG2 cells. Furthermore, we estimated the intracellular signaling pathway for the induction of SOD-2 mRNA expression. HepG2 cells were stimulated using extracts of four crops, including SQ, for 12 h; only extracts of colored potatoes induced SOD-2 mRNA expression significantly. This induction of SOD-2 mRNA expression was blocked by an inhibitor of the extracellular signal-related kinase (ERK) 1/2 pathway. Furthermore, an extract of SQ increased the phosphorylation of ERK1/2 after 15 or 30 min of stimulation. These data indicate that an extract of SQ directly induces hepatic SOD-2 mRNA expression via activation of ERK1/2 pathway in HepG2 cells.
Hashimoto
Naoto
N
Memuro Upland Farming Research Station, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido 082-0081, Japan. hasshy@affrc.go.jp
Noda
Takahiro
T
Kim
Sun-Ju
SJ
Yamauchi
Hiroaki
H
Takigawa
Shigenobu
S
Matsuura-Endo
Chie
C
Suzuki
Tatsuro
T
Han
Kyu-Ho
KH
Fukushima
Michihiro
M
D001947:Breeding; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D010766:Phosphorylation; D028321:Plant Preparations / Q000494:pharmacology*; D035281:Plant Tubers; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects; D011198:Solanum tuberosum / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D000099156:Superoxide Dismutase 2
D019950:Mitogen-Activated Protein Kinase 1; D028321:Plant Preparations; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2