Inescapable but not escapable stress leads to increased struggling behavior and basolateral amygdala c-fos gene expression in response to subsequent novel stress challenge.
Control over an aversive experience can greatly impact the organism's response to subsequent stressors. We compared the effects of escapable (ES) and yoked inescapable (IS) electric tail shocks on the hypothalamic-pituitary-adrenal (HPA) axis hormonal (corticosterone and adrenocorticotropic hormone (ACTH)), neural (c-fos mRNA) and behavioral (struggling) response to subsequent restraint. We found that although the HPA axis response during restraint of both previously stressed groups were higher than stress-naïve rats and not different from each other, lack of control over the tailshock experience led to an increase in restraint-induced struggling behavior of the IS rats compared to both stress-naïve and ES rats. Additionally, c-fos expression in the basolateral amygdala was increased selectively in the IS group, and relative c-fos mRNA expression in the basolateral amygdala positively correlated with struggling behavior. Restraint-induced c-fos expression in the medial prefrontal cortex, a brain area critical for mediating some of the differential neurochemical and behavioral effects of ES and IS, was surprisingly similar in both ES and IS groups, lower than that of stress-naïve rats, and did not correlate with struggling behavior. Our findings indicate that basolateral amygdala activity may be connected with the differential effects of ES and IS on subsequent behavioral responses to restraint, without contributing to the concurrent HPA axis hormone response.
Weinberg
M S
MS
Department of Psychology and Neuroscience, University of Colorado at Boulder, Boulder, CO 80309, USA.
Grissom
N
N
Paul
E
E
Bhatnagar
S
S
Maier
S F
SF
Spencer
R L
RL
D000679:Amygdala; D000818:Animals; D015331:Cohort Studies; D004558:Electric Stimulation / Q000379:methods; D004924:Escape Reaction / Q000502:physiology*; D005786:Gene Expression Regulation; D007103:Immobilization / Q000379:methods / Q000502:physiology* / Q000523:psychology; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000379:methods / Q000502:physiology / Q000523:psychology; D013315:Stress, Psychological / Q000235:genetics / Q000378:metabolism* / Q000523:psychology
D016760:Proto-Oncogene Proteins c-fos