Silver nanoparticles on a plastic platform for localized surface plasmon resonance biosensing.
A cost-effective fabrication method for the preparation of localized surface plasmon resonance (LSPR) biosensors supported on plastics is described. The silver-nanoparticles-on-plastic sensor (SNOPS) was fabricated by chemically modifying the surface of a common plastic, polyethylene terephthalate (PET) to allow the efficient immobilization of Ag NPs. The LSPR of the SNOPS strip showed good sample-to-sample reproducibility. The analytical performance of the sensor strips for monitoring both thiol and protein adsorption, including bioaffinity, was examined. The limit of quantification to the adsorption of 11-mercaptoundecanoic acid was 500 nM and for the detection of streptavidin was approximately 9.5 nM. SNOPS can then be used as a cheap, versatile, and yet sensitive LSPR biosensor.
Fan
Meikun
M
Thompson
Matthew
M
Andrade
Maria Luiza
ML
Brolo
Alexandre G
AG
D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D011093:Polyethylene Terephthalates / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D019809:Streptavidin / Q000032:analysis; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D011093:Polyethylene Terephthalates; D013438:Sulfhydryl Compounds; D012834:Silver; D001710:Biotin; D019809:Streptavidin