title: [Inhibitory effect of photodynamic therapy combined with paclitaxel on the proliferation of esophageal carcinoma Eca-109 cells].
abstract: OBJECTIVE
  To investigate the inhibitory effect of photodynamic therapy (PDT) in combination with paclitaxel (PCT) on proliferation in esophageal carcinoma Eca-109 cells line.
  
  
  METHODS
  Eca-109 cells were treated with PCT alone, HPD alone at different doses, or their combinations. For the combined treatments, the cells were exposed to PCT for 12 h followed by incubation with HPD at high, middle or low concentrations for 4 h. PDT was then performed on these treated cells and fluorescence microscopic observation was made before and after PDT. The cell survival was measured by MTT assay, and the cell apoptosis rate analyzed by flow cytometry after a 24-h cell incubation following PDT.
  
  
  RESULTS
  The fluorescence excitation of the cells was weakened after PDT. Combined treatments resulted in significantly lowered cell survival rate and increased cell apoptosis rates as compared to those of the control cells and the cells treated with PCT alone and low-dose HPD (P<0.01). Significant differences were also noted among the cells exposed to HPD at different concentrations (P<0.05).
  
  
  CONCLUSIONS
  PDT combined with PCT have significant synergetic effects in inhibiting the proliferation of human esophageal carcinoma cells and inducing their apoptosis in vitro.
authors.affiliation: Department of Chemotherapy, First Affiliated Hospital of Fujian Medical University, Fuzhou 35005, China. zzfhl2004@126.com
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Xi
authors.initials: X
authors.lastname: Shi
authors.forename: Ri-xiong
authors.initials: RX
authors.lastname: Wang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Lin
authors.forename: Xue-de
authors.initials: XD
authors.lastname: Lin
authors.forename: Hang
authors.initials: H
authors.lastname: Zheng
authors.forename: Li-bo
authors.initials: LB
authors.lastname: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D017239:Paclitaxel / Q000494:pharmacology*; D010778:Photochemotherapy
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel