title: Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01.
abstract: Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, reducing power assay, hydroxyl (.OH) and superoxide radicals (.O(2)(-)) assays, in which the last two assays were measured by electron spin resonance (ESR). There were two kinds of B. animalis 01 proteins in this study, the regular B. animalis 01 protein (Pro-CK) and the B. animalis 01 selenium-contained protein (Pro-Se). Both Pro-CK and Pro-Se showed concentration dependent antioxidant activity in DPPH assay, reducing power assay and erythrocyte hemolysis assay. All results of six assays indicated that the antioxidant activity of the B. animalis 01 protein was improved remarkably after selenium was incorporated. The antioxidant activity of Pro-Se increased with the increase of selenium content in Pro-Se suggesting selenium played a positive role in enhancing the antioxidant activity of B. animalis 01 protein. Moreover, organic selenium was more effective than inorganic selenium on enhancing the hydroxyl radical scavenging ability of B. animalis 01 protein.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Dong East Road, Haidian, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.forename: Bowen
authors.initials: B
authors.lastname: Zhang
authors.forename: Rihua
authors.initials: R
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuelong
authors.initials: X
authors.lastname: Ding
authors.forename: Pinglan
authors.initials: P
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D001644:Bifidobacterium / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D010084:Oxidation-Reduction; D010851:Picrates / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D012643:Selenium