Bcl-2 protein expression in egyptian acute myeloid leukemia.
OBJECTIVE
The primary cause of treatment failure in acute myeloid leukemia (AML) is the emergence of both resistant disease and early relapse. The bcl-2 gene encodes a 26-kDa protein that promotes cell survival by blocking programmed cell death (apoptosis). In the present study, bcl-2 protein expression was evaluated in newly diagnosed AML patients and correlated with the induction of remission and overall survival (OS), in an attempt to define patients who might benefit from modified therapeutic strategies.


METHODS
Pretreatment cellular bcl-2 protein expression was measured in bone marrow samples obtained from 68 patients of newly diagnosed acute myeloid leukemia and 10 healthy controls by western blotting.


RESULTS
The mean bcl-2 protein expression was significantly higher in patients (0.686+/-0.592) compared to controls (0.313+/-0.016) (p=0.002). The overall survival for patients with mean bcl-2 expression of less, and more than or equal to 0.315, was 67% and 56%, respectively, with no significant difference between the two groups (p=0.86).


CONCLUSIONS
Even though we did not observe a significant difference in overall survival between patients with high and low levels of bcl-2, modulation of this protein might still be considered as an option for enhancing the effectiveness of conventional chemotherapy.


BACKGROUND
Acute myeloid leukemia (AML) - Bcl-2 - prognosis - Western blot.
El-Shakankiry
Nayera H
NH
The Departments of Clinical Pathology, National Cancer Institute,Cairo University.
El-Sayed
Ghada M
GM
El-Maghraby
Shereen
S
Moneer
Manar M
MM