title: A photoactivatable marker protein for pulse-chase imaging with superresolution.
abstract: IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).
authors.affiliation: Institute of Applied Physics and Center for Functional Nanostructures, Karlsruhe Institute of Technology, Karlsruhe, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Fuchs
authors.forename: Susan
authors.initials: S
authors.lastname: Böhme
authors.forename: Franz
authors.initials: F
authors.lastname: Oswald
authors.forename: Per Niklas
authors.initials: PN
authors.lastname: Hedde
authors.forename: Maike
authors.initials: M
authors.lastname: Krause
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiedenmann
authors.forename: G Ulrich
authors.initials: GU
authors.lastname: Nienhaus
mesh_terms: D015415:Biomarkers; D003116:Color; D003952:Diagnostic Imaging / Q000379:methods; D005453:Fluorescence; D008164:Luminescent Proteins / Q000528:radiation effects*; D008853:Microscopy / Q000379:methods*; D055668:Photochemical Processes; D011868:Radioisotopes
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D011868:Radioisotopes