[Spectrophometric analysis of volatile compounds in microorganisms].
A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial growth at a concentration above 10(7) cells/ml. In addition, the method allows discriminating mutants of the fungus Neurospora crassa defective in the nitrogen metabolism from the wild type strains. It is likely that nitrite reductase and nitrate reductase enzymes regulated by the nit-2 and nit-6 genes are involved in formation of the water soluble volatile compounds of this organism.
Voloshin
A G
AG
Filippovich
S Iu
SIu
Bachurina
G P
GP
Besaeva
S G
SG
Ignatov
S G
SG
D001413:Bacillus thuringiensis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D050901:Nitrate Reductase / Q000235:genetics / Q000378:metabolism; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C068100:NIT2 protein, Neurospora crassa; D014157:Transcription Factors; D055549:Volatile Organic Compounds; D014867:Water; D009572:Nitrite Reductases; D050901:Nitrate Reductase