Preparation and characterization of ceria-zirconia composite for enrichment and identification of phosphopeptides.
Ceria-zirconia composites at different molar ratios were synthesized. Several methods were used to characterize these composites, including X-ray photoelectron spectroscopy, surface area and surface acid-base property detection. A one-step method for isolation and identification of phosphopeptides from peptide mixture was created using these ceria-zirconia composites. Using tryptic digest of standard phosphorylated protein, we have shown that these enrichment and dephosphorylation activities are effective. The adsorption capacity and catalytic property of ceria-zirconia composites at different molar ratios and calcinated temperatures were studied. In combination with MALDI-TOF-based peptide mass finger printing technique, we have established a method to utilize the enrichment/dephosphorylation dual properties of these ceria-zirconia composites for the analysis of phosphoprotein in nonfat milk successfully.
Wu
Jian-Hong
JH
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, P. R. China.
Xiao
Kuang
K
Zhao
Yong
Y
Zhang
Wei-Ping
WP
Guo
Lin
L
Feng
Yu-Qi
YQ
D002563:Cerium / Q000737:chemistry*; D010316:Particle Size; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015040:Zirconium / Q000737:chemistry*
D010748:Phosphopeptides; D002563:Cerium; C030583:ceric oxide; D015040:Zirconium; C028541:zirconium oxide