Potential of the TCE-degrading endophyte Pseudomonas putida W619-TCE to improve plant growth and reduce TCE phytotoxicity and evapotranspiration in poplar cuttings.
The TCE-degrading poplar endophyte Pseudomonas putida W619-TCE was inoculated in poplar cuttings, exposed to 0, 200 and 400 mg l(-1) TCE, that were grown in two different experimental setups. During a short-term experiment, plants were grown hydroponically in half strength Hoagland nutrient solution and exposed to TCE for 3 days. Inoculation with P. putida W619-TCE promoted plant growth, reduced TCE phytotoxicity and reduced the amount of TCE present in the leaves. During a mid-term experiment, plants were grown in potting soil and exposed to TCE for 3 weeks. Here, inoculation with P. putida W619-TCE had a less pronounced positive effect on plant growth and TCE phytotoxicity, but resulted in strongly reduced amounts of TCE in leaves and roots of plants exposed to 400 mg l(-1) TCE, accompanied by a lowered evapotranspiration of TCE. Dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), which are known intermediates of TCE degradation, were not detected.
Weyens
Nele
N
Hasselt University, Centre for Environmental Sciences, Agoralaan Building D, B-3590 Diepenbeek, Belgium.
Truyens
Sascha
S
Dupae
Joke
J
Newman
Lee
L
Taghavi
Safiyh
S
van der Lelie
Daniel
D
Carleer
Robert
R
Vangronsveld
Jaco
J
D001673:Biodegradation, Environmental; D003999:Dichloroacetic Acid / Q000378:metabolism; D018526:Plant Transpiration / Q000187:drug effects*; D032107:Populus / Q000187:drug effects* / Q000254:growth & development / Q000382:microbiology; D016958:Pseudomonas putida / Q000302:isolation & purification / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D013559:Symbiosis; D014238:Trichloroacetic Acid / Q000378:metabolism; D014240:Trichloroethanes / Q000378:metabolism* / Q000633:toxicity; D052584:Xylem / Q000378:metabolism / Q000382:microbiology
D012989:Soil Pollutants; D014240:Trichloroethanes; D014238:Trichloroacetic Acid; D003999:Dichloroacetic Acid