Protein adsorption on a glycosylated polyacrylonitrile surface: monitoring with QCM and SPR.
A simple and efficient method to fabricate a glycosylated surface on a polyacrylonitrile-based film is described. Construction and protein adsorption processes were monitored in situ using a QCM. A PANCHEMA film was deposited on the gold surface of the quartz crystal, and the glycosylated surface was then constructed through surface modification. Con A and BSA were used as probes to study the specificity of this surface to proteins. It can recognize Con A, while showing no specific interaction with BSA. The binding affinity indicates the presence of strong multivalent interactions between Con A and the glucose residues (cluster glycoside effect). Reproducibility and repeatability of the glycosylated polymer surface are sufficient to allow specific adsorption of Con A.
Che
Ai-Fu
AF
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Huang
Xiao-Jun
XJ
Xu
Zhi-Kang
ZK
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D002240:Carbohydrate Sequence; D006031:Glycosylation; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance / Q000379:methods*
D000180:Acrylic Resins; D011506:Proteins; C010504:polyacrylonitrile