[Genome sequencing and analysis of the bovine viral diarrhea virus-2 strain JZ05-1 isolated in China].
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, which is a widespread problem for beef and dairy herds, and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin, China generated cytopathic effect (CPE) in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced, the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results, the JZ05-1 genome was composed of 12285 nucleotides in length (GenBank accession No. GQ888686), which could be divided into three regions: a 387 nt 5'-untranslated region (UTR), a 11694 nt single large open reading frame encoding a polyprotein, and a 204 nt 3'-UTR. The 5'-UTR and genome sequences were analyzed by sequence alignment and construction of phylogenetic trees. The strain JZ05-1 was classified as BVDV type 2a. The BVDV-2 strain JZ05-1 genome showed high similarity to the p11Q isolated in Canada and the XJ-04 isolated in China, with 90% and 91% identity in nucleotide sequence, respectively. Compared with the similarity within the BVDV-2 genotype (96%), the JZ05-1 had low sequence similarity to other BVDV-2 strains.
Li
Qing-chao
QC
Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109, China. Sman9@126.com
Miao
Li-guang
LG
Li
Hai-tao
HT
Liu
Yan-huan
YH
Zhang
Guang-lei
GL
Xiao
Jia-mei
JM
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002417:Cattle; D002681:China; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D020121:5' Untranslated Regions