Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation.
Lantanacamara contains 61.1% (w/w) holocellulose and can serve as a low-cost feedstock for bioethanol production. Acid hydrolysis (3.0%, v/v H(2)SO(4), 120 degrees C for 45 min) of L. camara produced 187.14 mg/g total sugars along with fermentation inhibitors such as phenolics (8.2mg/g), furfurals (5.1mg/g) and hydroxy methyl furfurals (6.7 mg/g). Sequential application of overliming (pH 10.0) and activated charcoal (1.5%, w/v) adsorption was used to remove these toxic compounds from the acid hydrolysate. The acid-pretreated biomass of L. camara was further delignified through combined pretreatment of sodium sulphite (5.0% w/v) and sodium chlorite (3.0% w/v), which resulted in about 87.2% lignin removal. The enzymatic hydrolysis of delignified cellulosic substrate showed 80.0% saccharification after 28 h incubation at 50 degrees C and pH 5.0. Fermentation of acid and enzymatic hydrolysates with Pichiastipitis and Saccharomycescerevisiae gave rise to 5.16 and 17.7 g/L of ethanol with corresponding yields of 0.32 and 0.48 g/g after 24 and 16 h, respectively.
Kuhad
Ramesh Chander
RC
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India. kuhad85@gmail.com
Gupta
Rishi
R
Khasa
Yogender Pal
YP
Singh
Ajay
A
D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D044602:Cellulases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D006868:Hydrolysis / Q000187:drug effects; D032410:Lantana / Q000187:drug effects / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013464:Sulfuric Acids / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology
D056804:Biofuels; D010636:Phenols; D013464:Sulfuric Acids; D000431:Ethanol; D008031:Lignin; D044602:Cellulases; C033158:sulfuric acid