title: Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses.
abstract: All 16 subtypes of avian influenza viruses of low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four 2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species: goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA) titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity of replicating LPAIV and LPAIV antigens decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer. Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that goose and guinea pig erythrocytes are the best in HA assay for all subtypes of influenza viruses.
authors.affiliation: Faculty of Veterinary Science, Mahidol University, Nakhonpathom 73170, Thailand.
authors.forename: Witthawat
authors.initials: W
authors.lastname: Wiriyarat
authors.forename: Hatairat
authors.initials: H
authors.lastname: Lerdsamran
authors.forename: Phisanu
authors.initials: P
authors.lastname: Pooruk
authors.forename: Robert G
authors.initials: RG
authors.lastname: Webster
authors.forename: Suda
authors.initials: S
authors.lastname: Louisirirotchanakul
authors.forename: Parntep
authors.initials: P
authors.lastname: Ratanakorn
authors.forename: Kridsada
authors.initials: K
authors.lastname: Chaichoune
authors.forename: Kannika
authors.initials: K
authors.lastname: Nateerom
authors.forename: Pilaipan
authors.initials: P
authors.lastname: Puthavathana
mesh_terms: D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology / Q000821:virology*; D005777:Geese; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000378:metabolism; D006736:Horses; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology / Q000378:metabolism; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000821:virology*
chemical_list: D006388:Hemagglutinins