Biodegradation of chlorimuron-ethyl by the bacterium Klebsiella jilinsis 2N3.
Enrichment culturing of sludge taken from an industrial wastewater treatment pond led to the identification of a bacterium (Klebsiella jilinsis H. Zhang) that degrades chlorimuron-ethyl with high efficiency. Klebsiella jilinsis strain 2N3 grows with chlorimuron-ethyl as the sole nitrogen source at the optimal temperature range of 30-35 degrees C and pH values between 6.0-7.0. In liquid medium, the degradation activity was further induced by chlorimuron-ethyl. Degradation rates followed the pesticide degradation kinetic equation at concentrations between 20 and 200 mg L(-1). Using initial concentrations of 20 and 100 mg L(-1), the degradation rates of chlorimuron-ethyl were 83.5 % and 92.5 % in 12 hours, respectively. At an initial concentration higher than 200 mg L(-1), the degradation rate decreased slightly as the concentration increased. The 2N3 strain also degraded the sulfonylurea herbicides ethametsulfuron, metsulfuron-methyl, nicosulfuron, rimsulfuron, and tribenuron-methyl. This study provides scientific evidence and support for the application of K. jilinsis in bioremediation to reduce environmental pollution.
Zhang
Hao
H
College of Resource and Environment, Jilin Agricultural University, Changchun, People's Republic of China.
Zhang
Xianghui
X
Mu
Wenhui
W
Wang
Jiaxiu
J
Pan
Hongyu
H
Li
Yu
Y
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007700:Kinetics; D007709:Klebsiella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010575:Pesticides / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D011743:Pyrimidines / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013453:Sulfonylurea Compounds / Q000737:chemistry / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010575:Pesticides; D011743:Pyrimidines; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013453:Sulfonylurea Compounds; C112343:chlorimuron ethyl