title: Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles.
abstract: In this study, a method for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC-FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid-0.2 m MgCl(2) in water and acetonitrile as an extraction solvent, and n-hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents.
authors.affiliation: Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, Republic of Korea.
authors.forename: Jeong-Heui
authors.initials: JH
authors.lastname: Choi
authors.forename: Tae Woong
authors.initials: TW
authors.lastname: Na
authors.forename: Md Iqbal Rouf
authors.initials: MI
authors.lastname: Mamun
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Eun-Ho
authors.initials: EH
authors.lastname: Shin
authors.forename: Jong-Hyouk
authors.initials: JH
authors.lastname: Park
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002021:Buffers; D005591:Chemical Fractionation / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005470:Fluorometry / Q000379:methods; D005504:Food Analysis / Q000379:methods; D006586:Hexanes / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000097:Acetonitriles; D002021:Buffers; D006586:Hexanes; C026385:n-hexane; D002939:Ciprofloxacin; C047224:sarafloxacin; C032159:acetonitrile