N-Glycosylation patterns of hemolymph glycoproteins from Biomphalaria glabrata strains expressing different susceptibility to Schistosoma mansoni infection.
Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (β1-2)-linked xylose or terminal Fuc(α1-3)GalNAc(β1-4)[±Fuc(α1-3)]GlcNAc(β1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host.
Lehr
Tobias
T
Institute of Biochemistry, Faculty of Medicine, University of Giessen, Giessen, Germany.
Frank
Sandra
S
Natsuka
Shunji
S
Geyer
Hildegard
H
Beuerlein
Knut
K
Doenhoff
Michael J
MJ
Hase
Sumihiro
S
Geyer
Rudolf
R
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology; D001700:Biomphalaria / Q000737:chemistry* / Q000276:immunology / Q000469:parasitology; D015153:Blotting, Western; D002033:Bulinus / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006031:Glycosylation; D006458:Hemolymph / Q000737:chemistry*; D007150:Immunohistochemistry; D011134:Polysaccharides / Q000276:immunology / Q000378:metabolism; D012550:Schistosoma mansoni / Q000276:immunology / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000947:Antigens, Helminth; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D011134:Polysaccharides