High frequency of the IL-2 -330 T/HLA-DRB1*1501 haplotype in patients with multiple sclerosis.
We have evaluated the role of the HLA-DRB1*1501 allele and the IL-2 -330 T/G polymorphism and their interaction in susceptibility to multiple sclerosis on 360 patients and 426 matched healthy individuals. We used the SSP-PCR method to determine the alleles. Fisher's exact test was used to analyses. We observed a significant increase in the T allele at IL-2 -330 position in patients (OR=1.34, P<0.05), and the T/T and T/G genotypes were more frequent among patients than controls. The HLA-DRB1*1501 allele was overrepresented in patients as compared to the control group (OR=1.7, P=0.0006). The two-locus analysis of the interaction between the IL-2 promoter polymorphism and the HLA-DRB1 allele showed that the HLA-DRB1*1501/T haplotype was more frequent in patients than controls (OR=16, P<0.0001). Our findings support previous findings about the role of the HLA-DRB1*1501 allele in susceptibility to MS. This work also provides new findings about the importance of gene-gene interactions in the development of MS.
Shahbazi
Majid
M
Medical Cellular & Molecular Research Center, Talghani Children Hospital of Golestan University of Medical Sciences, Bolv Janbazan, 4916668197, Gorgan, Iran. shahbazimajid@yahoo.co.uk
Roshandel
Danial
D
Ebadi
Hamid
H
Fathi
Davood
D
Zamani
Mahdi
M
Boghaee
Mojdeh
M
Mohammadhoseeeni
Mana
M
Rshaidbaghan
Azam
A
Bakhshandeh
Azam
A
Shahbazi
Saleh
S
D000328:Adult; D017668:Age of Onset; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012720:Severity of Illness Index; D055815:Young Adult
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C489631:HLA-DRB1*15:01 antigen; D007376:Interleukin-2