title: Structural insights to investigate Conypododiol as a dual cholinesterase inhibitor from Asparagus adscendens.
abstract: The main aim of the current study was to explore molecular insights for potentially new dual cholinesterase inhibitor(s) from Asparagus adscendens via molecular docking. This medicinal plant is traditionally used as a nerve tonic and remedy for memory impairments. Conypododiol was isolated from the chloroform fraction of methanolic extract of A. adscendens, based on bioactivity guided isolation. Conypododiol exhibited significant inhibition of both acetylcholinesterase and butyrylcholinesterase, having the IC(50) values 2.17 ± 0.1 μM and 11.21 ± 0.1 μM, respectively. IC(50) values of the standard compound Galanthamine for both the enzymes were 0.537 ± 0.018 μM and 8.6 ± 0.27 μM, respectively. Based on MTT cytotoxicity assay, Conypododiol was found safe against LCMK-2 monkey kidney epithelial cells and mice hepatocytes. Molecular docking studies revealed the hydrogen bonding interactions of Conypododiol with His440 and Ser200 at esteratic site (ES), and also with Tyr334 at peripheral anionic site (PAS) of the aromatic gorge of acetylcholinesterase. Simultaneous contacts of Conypododiol with PAS and ES shows its significance as a bivalent ligand. This preliminary study highlighted the potential of Conypododiol to be further developed and modified as new lead compound identified by its folk use.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan. inam_marwat333@yahoo.com
authors.forename: Inamullah
authors.initials: I
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nisar
authors.forename: Nematullah
authors.initials: N
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saeed
authors.forename: Said
authors.initials: S
authors.lastname: Nadeem
authors.lastname: Fazal-ur-Rehman
authors.forename: Farooq
authors.initials: F
authors.lastname: Ali
authors.forename: Nasiara
authors.initials: N
authors.lastname: Karim
authors.forename: Waqar Ahmad
authors.initials: WA
authors.lastname: Kaleem
authors.forename: Mughal
authors.initials: M
authors.lastname: Qayum
authors.forename: Hanif
authors.initials: H
authors.lastname: Ahmad
authors.forename: Ihsan Ali
authors.initials: IA
authors.lastname: Khan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000475:Alkenes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D027761:Asparagus Plant / Q000737:chemistry*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D005663:Furans / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure
chemical_list: D000475:Alkenes; D002800:Cholinesterase Inhibitors; D005663:Furans; C556845:conypododiol; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase