title: [Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells].
abstract: OBJECTIVE
  To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.
  
  
  METHODS
  Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.
  
  
  RESULTS
  After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05).
  
  
  CONCLUSIONS
  Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.
authors.affiliation: Department of Surgery, Guangzhou Red-cross Hospital, Jinan Medical University, Guangzhou 510220, China. william0406@163.com
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Wu
authors.forename: Chi-hua
authors.initials: CH
authors.lastname: Fang
authors.forename: Zheng-xin
authors.initials: ZX
authors.lastname: Yang
authors.forename: Jia-jin
authors.initials: JJ
authors.lastname: Bao
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D066246:ErbB Receptors; D006821:Hyaluronoglucosaminidase