[Extraction and antihypertensive activity analysis of chondroitin sulfate from different animals].
OBJECTIVE
Chondroitin Sulfate (CS) from different animals were extracted, which antihypertensive activities were compared.


METHODS
CS from the bovine and chicken cartilages were extracted by diluted alkali-enzyme hydrolysis method, with removed free protein by Sevag method, separated and purified by quaternary ammonium complex. Their antihypertensive activities were tested by the modal of SPF rat.


RESULTS
The extracted CS didn't have peptide, amino acid or other acid mucopolysaccharides determined by electrophoresis and chromatography, the results of IR was consisted with that of chondroitin sulfate supplied by Sigma Both BCCS and CCCS indicated the antihypertensive activities in the low dosage. Besides, BCCS had faster efficacy but shorter duration than that of CCCS.


CONCLUSIONS
Both BCCS and CCCS had high purity. Animal experiments showed that BCCS and CCCS have the effect of the antihypertensive, which activity of CCCS was more significant than that of BCCS.
Liu
Sha
S
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Shen
Jian-ru
JR
Yu
Rong-min
RM
D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects*; D002356:Cartilage / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D012272:Ribs; D012540:Scapula; D013678:Technology, Pharmaceutical / Q000379:methods
D000959:Antihypertensive Agents; D002809:Chondroitin Sulfates