title: Repairing collagen in dentin carious lesions. Influence of sealing the material: a morphometric study.
abstract: OBJECTIVE
  The aim of the present work was to morphometrically evaluate collagen in carious lesions sealed with calcium hydroxide, adhesive systems, glass ionomer cement, and an antibacterial cement.
  
  
  METHODS
  Samples of infected and affected dentin were stained with Sirius Red (SR). The areas intensively stained with SR were delimited, and the percentage of these areas was measured by blind calibrated examiners. The mean results were subjected to the Kruskal-Wallis test.
  
  
  RESULTS
  The affected dentin sealed with Ca(OH)2 showed a better organization of the collagen in relation to the adhesive systems Prime & Bond (p = .0159) and Adhese (p < 0.0001). The affected dentin sealed with Prime & Bond promoted better increase of organized collagen areas in relation to Adhese (p = 0.0004). The infected dentin sealed with glass ionomer cement (p = 0.0018) or antibacterial cement (p = 0.0004) brought a significant increase in the organized collagen areas.
  
  
  CONCLUSIONS
  Ca(OH)2 is indicated to seal affected dentin and glass ionomer cement and antibacterial cement may be used for treatment of infected dentin. The addition of antibiotics did not influence the restoration of the infected dentin.
authors.affiliation: Pontifícia Universidade Católica de Campinas - PUC - Campinas, Brazil. psergio@usp.br
authors.forename: Sérgio Luiz
authors.initials: SL
authors.lastname: Pinheiro
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: Gallassi
authors.forename: Talita Costa
authors.initials: TC
authors.lastname: Saldanha
authors.forename: Iris Nogueira
authors.initials: IN
authors.lastname: Bincelli
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Barros
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Silva
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002126:Calcium Hydroxide / Q000737:chemistry; D003094:Collagen / Q000648:ultrastructure*; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D057087:Dental Atraumatic Restorative Treatment; D001840:Dental Bonding; D003731:Dental Caries / Q000473:pathology / Q000628:therapy*; D003738:Dental Cements / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000473:pathology*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D005899:Glass Ionomer Cements / Q000737:chemistry; D006801:Humans; D008963:Molar / Q000473:pathology; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D014094:Tooth, Deciduous / Q000473:pathology; D015040:Zirconium / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; C489662:AdheSE cement; D000900:Anti-Bacterial Agents; D004396:Coloring Agents; D003188:Composite Resins; D003738:Dental Cements; D003764:Dental Materials; D017220:Dentin-Bonding Agents; D005899:Glass Ionomer Cements; D011109:Polymethacrylic Acids; D019279:Resin Cements; C090880:Vidrion F; C083568:Z100 composite resin; C444443:prime and bond 2.0; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D003094:Collagen; D015040:Zirconium; D002126:Calcium Hydroxide