title: Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters.
abstract: Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these conditions.
authors.affiliation: Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D-52426 Juelich, Germany. t.drepper@fz-juelich.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Drepper
authors.forename: Robert
authors.initials: R
authors.lastname: Huber
authors.forename: Achim
authors.initials: A
authors.lastname: Heck
authors.forename: Franco
authors.initials: F
authors.lastname: Circolone
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Hillmer
authors.forename: Jochen
authors.initials: J
authors.lastname: Büchs
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
chemical_list: D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins; D005486:Flavin Mononucleotide; D010100:Oxygen