Production and characterization of agonistic monoclonal antibodies against chicken CD40.
CD40 is mainly expressed by professional antigen-presenting cells (APCs). Its ligand, CD40L, is transiently expressed on activated CD4(+) T-cells. CD40-CD40L interactions mediate T-cell help to APCs and provide crucial signals for affinity maturation and B-cell class switching. In mammals, agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming and expansion, increased antibody production and class switching. In this study, we describe agonistic anti-chicken CD40 mAb 2C5. This mAb detected CD40 on primary chicken B-cells and macrophages, DT40 B-cells, and HD11 macrophages, induced NO synthesis in HD11 macrophages, and stimulated DT40 B-cell proliferation. These observations demonstrated at least partial functional equivalence of 2C5 to chicken CD154. This mAb may therefore constitute a new tool to study the role of CD40 in the chicken immune system, and its agonistic effects suggest that it could also be used as an adjuvant.
Chen
Chang-Hsin
CH
Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA.
Abi-Ghanem
Daad
D
Njongmeta
Leo
L
Bray
Jocelyn
J
Mwangi
Waithaka
W
Waghela
Suryakant D
SD
McReynolds
Jackson L
JL
Ing
Nancy H
NH
Berghman
Luc R
LR
D000276:Adjuvants, Immunologic; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D030161:Avian Proteins / Q000819:agonists / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019013:CD40 Antigens / Q000819:agonists / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D023201:CD40 Ligand / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D005818:Genetic Engineering; D008024:Ligands; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D019013:CD40 Antigens; D008024:Ligands; D023201:CD40 Ligand; D009569:Nitric Oxide