title: MUTYH Tyr165Cys, OGG1 Ser326Cys and XPD Lys751Gln polymorphisms and head neck cancer susceptibility: a case control study.
abstract: In the present study we investigated the association between three polymorphisms of the MUTYH (Tyr165Cys, rs34612342), the OGG1 (Ser326Cys, rs1052133) and the XPD (Lys751Gln, rs13181) genes with head and neck cancer risk. Genotypes were determined in DNA from peripheral blood lymphocytes of 265 patients with head and neck squamous cell carcinoma (HNSCC) as well as 280 cancer-free controls by PCR-restriction fragment length polymorphisms. We found an association between HNSCC and the Ser326Cys (OR 1.69; 95% CI 1.19-2.45) as well as Cys326Cys (OR 4.56; 95% CI 2.07-10.05) variants of the OGG1 gene. The gene-gene interaction between MUTYH and OGG1 as well as OGG1 and XPD polymorphic variants may contribute to higher prevalence of HNSCC. We also found an association between Ser326Cys and Cys326Cys variants of OGG1 gene and smoking status in HNSCC patients (OR 1.97; 95% CI 1.25-3.11), (OR 3.54; 95% CI 1.39-9.04), respectively. Moreover, we also observed a protective association between Tyr165Cys variant of the MUTYH gene and non-smoking status in HNSCC (OR 0.34; 95% CI 0.17-0.66). We also found a link between gene-gene interaction (MUTYH and OGG1 or OGG1 and XPD) and smoking (ORs 2.17-4.20 and 2.18-5.23) or non-smoking status (ORs 0.11 and 7.61) in HNSCC patients, respectively. In conclusion our data showed that the Ser326Cys polymorphism of the OGG1 gene may modify the risk of HNSCC associated with smoking. Finally we suggested that this polymorphism might be used as predictive factor for head and neck cancer in Polish population.
authors.affiliation: Department of Molecular Genetics, University of Lodz, 12/16, 90-237, Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sliwinski
authors.forename: Karolina
authors.initials: K
authors.lastname: Przybylowska
authors.forename: Lukasz
authors.initials: L
authors.lastname: Markiewicz
authors.forename: Pawel
authors.initials: P
authors.lastname: Rusin
authors.forename: Wioletta
authors.initials: W
authors.lastname: Pietruszewska
authors.forename: Hanna
authors.initials: H
authors.lastname: Zelinska-Blizniewska
authors.forename: Jurek
authors.initials: J
authors.lastname: Olszewski
authors.forename: Alina
authors.initials: A
authors.lastname: Morawiec-Sztandera
authors.forename: Wojciech
authors.initials: W
authors.lastname: Mlynarski
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Majsterek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D045647:DNA Glycosylases / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012907:Smoking; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D045647:DNA Glycosylases; C061738:mutY adenine glycosylase; C479058:oxoguanine glycosylase 1, human; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human