title: False-positive transcription-mediated amplification assay detection of West Nile virus in blood from a patient with viremia caused by an Usutu virus infection.
abstract: Detection of West Nile virus (WNV) by nucleic acid amplification technology (NAAT) is used widely to screen blood and organ donations in areas where WNV is endemic. We report a false-positive result of a WNV transcription-mediated amplification assay (TMA) in a patient with viremia that was caused by Usutu virus, a mosquito-borne flavivirus.
authors.affiliation: Department of Haematology and Oncology L. and A. Seragnoli, Unit of Clinical Microbiology, Regional Reference Centre for Microbiological Emergencies (CRREM), S. Orsola-Malpighi Hospital, University of Bologna, 9 via G. Massarenti, 40138 Bologna, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Gaibani
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Pierro
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavrini
authors.forename: Giada
authors.initials: G
authors.lastname: Rossini
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Landini
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sambri
mesh_terms: D001769:Blood / Q000821:virology; D018349:Encephalitis Viruses, Japanese / Q000302:isolation & purification*; D004671:Encephalitis, Arbovirus / Q000175:diagnosis* / Q000821:virology; D005189:False Positive Reactions; D018177:Flavivirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D014766:Viremia; D014773:Virology / Q000379:methods*; D014901:West Nile Fever / Q000175:diagnosis / Q000821:virology; D014902:West Nile virus / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral