title: Comprehensive and innovative techniques for liver transplantation in rats: a surgical guide.
abstract: OBJECTIVE
  To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.
  
  
  METHODS
  We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.
  
  
  RESULTS
  Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis.
  
  
  CONCLUSIONS
  OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.
authors.affiliation: Department of Neuroscience, Mayo Clinic, Birdsall Research Building, Room 323, 4500 San Pablo Road, Jacksonville, Florida, FL 32224, United States. horit@kuhp.kyoto-u.ac.jp
authors.forename: Tomohide
authors.initials: T
authors.lastname: Hori
authors.forename: Justin H
authors.initials: JH
authors.lastname: Nguyen
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhao
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ogura
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hata
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yagi
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Ann-Marie T
authors.initials: AM
authors.lastname: Baine
authors.forename: Norifumi
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authors.lastname: Ohashi
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Eckman
authors.forename: Aimee R
authors.initials: AR
authors.lastname: Herdt
authors.forename: Hiroto
authors.initials: H
authors.lastname: Egawa
authors.forename: Yasutsugu
authors.initials: Y
authors.lastname: Takada
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Oike
authors.forename: Seisuke
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Mureo
authors.initials: M
authors.lastname: Kasahara
authors.forename: Kohei
authors.initials: K
authors.lastname: Ogawa
authors.forename: Koichiro
authors.initials: K
authors.lastname: Hata
authors.forename: Taku
authors.initials: T
authors.lastname: Iida
authors.forename: Yukihide
authors.initials: Y
authors.lastname: Yonekawa
authors.forename: Lena
authors.initials: L
authors.lastname: Sibulesky
authors.forename: Kagemasa
authors.initials: K
authors.lastname: Kuribayashi
authors.forename: Takuma
authors.initials: T
authors.lastname: Kato
authors.forename: Kanako
authors.initials: K
authors.lastname: Saito
authors.forename: Linan
authors.initials: L
authors.lastname: Wang
authors.forename: Mie
authors.initials: M
authors.lastname: Torii
authors.forename: Naruhiko
authors.initials: N
authors.lastname: Sahara
authors.forename: Naoko
authors.initials: N
authors.lastname: Kamo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sahara
authors.forename: Motohiko
authors.initials: M
authors.lastname: Yasutomi
authors.forename: Shinji
authors.initials: S
authors.lastname: Uemoto
mesh_terms: D000818:Animals; D006085:Graft Survival; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000601:surgery; D016031:Liver Transplantation / Q000295:instrumentation / Q000379:methods*; D023421:Models, Animal; D051381:Rats; D011917:Rats, Inbred Lew; D015996:Survival Rate; D016896:Treatment Outcome