Backbone assignment of the catalytic core of a Y-family DNA polymerase.
Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase, bypasses DNA lesions. Here, we report the assignments for the backbone nitrogen, carbon, and amide proton NMR signals of Dpo4's catalytic core consisting of the finger, palm, and thumb domains. Our work provides the basis for further NMR spectroscopic studies of the interactions among Dpo4, DNA, and an incoming nucleotide.
Ma
Dejian
D
Department of Biochemistry, The Ohio State University, 880 Biological Sciences, 484 West 12th Ave., Columbus, OH 43210, USA.
Fowler
Jason D
JD
Yuan
Chunhua
C
Suo
Zucai
Z
D000595:Amino Acid Sequence; D020134:Catalytic Domain; D019951:DNA Polymerase beta / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D048229:Sulfolobus solfataricus / Q000201:enzymology*
D019951:DNA Polymerase beta