title: Histopathological analysis of initial cellular response in TLR-2 deficient mice experimentally infected by Leishmania (L.) amazonensis.
abstract: Tegumentary leishmaniasis is an important public health problem in several countries. The capacity of the Leishmania species, at the initial moments of the infection, to invade and survive inside the host cells involves the interaction of surface molecules that are crucial in determining the evolution of the disease. Using C57BL/6 wild-type and TLR-2(-/-) mice infected with L. (L.) amazonensis, we demonstrated that TLR-2(-/-) mice presented eosinophilic granuloma in the ear dermis, different from C57BL/6 wild-type mice that presented a cellular profile characterized mainly by mononuclear cell infiltrates, besides neutrophils and eosinophils, during the two first week of infection. When the parasite load was evaluated, we found that the absence of TLR-2 lead to a significant reduction of the infection in deficient mice, when compared with C57BL/6 mice which were more susceptible to the infection. Using TLR-2 deficient mice, it was possible to show that the absence of this receptor determined the reduction of the parasite load and the recruitment of inflammatory cells during the two first weeks after L. (L.) amazonensis infection.
authors.affiliation: Laboratory of Structural Biology, Oswaldo Cruz Institute, FIOCRUZ - Rio de Janeiro/RJ-Brazil.
authors.forename: Camila Silva
authors.initials: CS
authors.lastname: Guerra
authors.forename: Roger Magno Macedo
authors.initials: RM
authors.lastname: Silva
authors.forename: Luís Otávio Pereira
authors.initials: LO
authors.lastname: Carvalho
authors.forename: Kátia da Silva
authors.initials: KS
authors.lastname: Calabrese
authors.forename: Patrícia Torres
authors.initials: PT
authors.lastname: Bozza
authors.forename: Suzana
authors.initials: S
authors.lastname: Côrte-Real
mesh_terms: D000818:Animals; D020405:Dermis / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D018450:Disease Progression; D005260:Female; D007891:Leishmania / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology*; D008264:Macrophages / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009504:Neutrophils / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000235:genetics* / Q000276:immunology
chemical_list: C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2