Silver sol immunoagglutination assay for determination of human IgG.
Immunoagglutination properties of silver nanoparticles sensitized with antibodies to human IgG for determination of IgG were studied. Effects of various pH on sensitization and consequent immunoagglutination process were investigated. It was shown that the best results were obtained when sensitization and immunoagglutination were conducted at isoelectric point of IgG molecule. It was demonstrated that immunoassay with colloidal silver nanoparticles provides the detection of antigen in wide range of concentrations.
Gasparyan
Vardan K
VK
Institute of Biochemistry, National Academy of Sciences, PSevak str 5/1, Yerevan 0014, Republic of Armenia. vgasparyan@excite.com
D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
D007074:Immunoglobulin G; D012834:Silver