title: Overexpressed Id-1 is associated with patient prognosis and HBx expression in hepatitis B virus-related hepatocellular carcinoma.
abstract: Id-1 is a member of the helix-loop-helix protein family and is involved in multiple biological processes, including development, proliferation, angiogenesis and carcinogenesis. However, the role of Id-1 in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is still unclear. In this study, Id-1 expression in 96 tumor specimens of HBV-related HCC was detected by immunohistochemical (IHC) staining. The relationship between the Id-1 expression grade and patient clinicopathological features was studied. In addition, the relationship between Id-1 expression and disease-free and overall survival times was analyzed. Colocalization of Id-1 and HBx was investigated by confocal laser scanning microscopy. The effect of HBx on Id-1 expression was studied in vitro using the HepG2 HCC cell line. Overexpression of Id-1 was found in 64.6% (62/96) of tumor specimens and was correlated with the histological grade, portal vein invasion, lymph node metastasis, HBsAg and Child-Pugh classification. Patients with Id-1 overexpression had both shorter disease-free and overall survival times. Besides, colocalization of Id-1 and HBx was found by paired IHC and confocal study. The expression of Id-1 was positively correlated to that of HBx. In vitro ectopic expression of HBx in HepG2 cells significantly increased Id-1 mRNA and protein expression. To our knowledge, this is the first report suggesting that Id-1 expression is at least partially regulated by HBx and may serve as a potential prognostic marker for HBV-related HCC.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Shaanxi Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ding
authors.forename: Shuang
authors.initials: S
authors.lastname: Han
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Changcun
authors.initials: C
authors.lastname: Guo
authors.forename: Huahong
authors.initials: H
authors.lastname: Xie
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Song
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Kefeng
authors.initials: K
authors.lastname: Dou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics / Q000821:virology*; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019694:Hepatitis B, Chronic / Q000378:metabolism*; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics / Q000821:virology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015534:Trans-Activators / Q000096:biosynthesis*; D054334:Viral Regulatory and Accessory Proteins; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein