[Effect of phosphatase PHLPP1 gene transfer on the proliferation of human umbilical vein endothelial cells].
OBJECTIVE
To investigate the constituent expression of PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1) in human umbilical vein endothelial cells (HUVECs) and the effect of PHLPP1 gene transfer on the proliferation of the cells in vitro.


METHODS
Cultured HUVECs were transfected with pcDNA3-GFP or pcDNA3HA-PHLPP1 via lipofectamine 2000. The cell proliferation ability was determined by cell counting and MTT colorimetric assay, and Western blotting was used to detect the protein expression of PHLPP1 in the cells.


RESULTS
No PHLPP1 protein was detected in the non-transfected cells or pcDNA3-GFP-transfected cells. pcDNA3HA-PHLPP1 gene transfection significantly increased PHLPP1 expression in the HUVECs (P<0.01), but the cell proliferation status remained unchanged (P>0.05). The absorbance of the cells measured by MTT assay was 0.134-/+0.0152, 0133-/+0.014 and 0.137-/+0.016, with cell counts of (8.293-/+0.962)x10(5), (7.937-/+0.101)x10(5) and (8.127-/+0.112)x10(5), respectively, showing no significant differences between the 3 groups (P>0.05).


CONCLUSIONS
Phosphatase PHLPP1 may not be the most important signal protein in the regulation of HUVEC proliferation.
Wu
Xing-li
XL
Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China. WXL301@yahoo.com
Yang
Ding-you
DY
Yan
Wei
W
Shang
Ai-jia
AJ
Lu
Cai-yi
CY
Xu
Hui-bin
HB
Wang
Shi-wen
SW
D049109:Cell Proliferation; D018014:Gene Transfer Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D014162:Transfection
D009687:Nuclear Proteins; C400601:PHLPP1 protein, human; D010749:Phosphoprotein Phosphatases