title: Effects of S100A9 in a rat model of asthma and in isolated tracheal spirals.
abstract: S100A9 is a member of the S100 family of proteins that contain two EF-hand calcium-binding motifs. We previously reported that S100A9 was differentially expressed during the early airway response phase of asthma and can be regulated by acupuncture. To understand the possible role of S100A9 in asthma, the effects of the S100A9 were investigated in a rat model of asthma and in isolated tracheal spirals. The pulmonary function and isometric tension were measured after the administration of purified recombinant S100A9. The results of in vivo experiments showed that S100A9 (0.1microg/kg) significantly decreased the pulmonary resistance and increased the dynamic compliance. The in vitro experimental results showed that S100A9 (100, 200, 400, or 800ng/ml, final concentrations) significantly reduced the isometric tension of isolated tracheal spirals. These results suggest that S100A9 elicits dose-dependent anti-asthmatic effects and may provide further insight into the treatment of asthma.
authors.affiliation: Laboratory of Molecular Biology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Zhang
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000403:Airway Resistance / Q000187:drug effects*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D001249:Asthma / Q000503:physiopathology / Q000628:therapy*; D040502:Calgranulin B / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D014132:Trachea / Q000187:drug effects
chemical_list: D018927:Anti-Asthmatic Agents; D040502:Calgranulin B; D011993:Recombinant Fusion Proteins