title: [A heterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens].
abstract: A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta laccase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing. Activity of the secreted heterologous laccase in the culture liquid reaches 3 U/ml, accounting for 98% of the total laccase activity, which demonstrates a high efficiency ofheterologous secretion. The synthesized P. canescens laccase has the same molecular weight as the enzyme produced by T. hirsuta 072.
authors.forename: A R
authors.initials: AR
authors.lastname: Abianova
authors.forename: A M
authors.initials: AM
authors.lastname: Chulkin
authors.forename: E A
authors.initials: EA
authors.lastname: Vavilova
authors.forename: T V
authors.initials: TV
authors.lastname: Fedorova
authors.forename: D S
authors.initials: DS
authors.lastname: Loginov
authors.forename: O V
authors.initials: OV
authors.lastname: Koroleva
authors.forename: S V
authors.initials: SV
authors.lastname: Benevolenskiĭ
mesh_terms: D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D042845:Laccase; D010407:Penicillium / Q000235:genetics / Q000254:growth & development*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D055454:Trametes / Q000201:enzymology* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D042845:Laccase