title: [Efficacy study in vitro: assessment of the properties of resveratrol and resveratrol + N-acetyl-cysteine on proliferation and inhibition of collagen activity].
abstract: During and after menopause the skin shows up clearly how the lack of estrogen affects tissues, and menopause can in fact be considered a "multisystemic" disorder of connective tissue. The low menopausal estrogen levels combined with age-related skin changes, accelerating skin aging. This affects both the epidermis and the dermis: fibroblasts not only become fewer, but they produce 30% less collagen, reflecting its metabolic decline. Estrogens act on collagen synthesis by directly stimulating fibroblasts. However, hormone replacement can prevent the postmenopausal loss of collagen--or eliminate it once it has started. The results of the Women's Health Initiative study drastically changed Italian gynecologists' prescribing habits. Natural products with estrogen-like activity are increasingly accepted, since they have good effects on collagen synthesis and/or inhibit collagenase activity, with a reassuring safety profile. This was confirmed by an in vitro study that assessed the tonic-trophic properties of two treatments on cultured skin fibroblasts. Cells were treated with resveratrol either alone or combined with NAC 10-100-1000 μM. There was a dose-related increase in the rate of cell proliferation and in inhibition of collagenase activity.
authors.affiliation: Farcoderm srl, San Martino Siccomario, Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Giardina
authors.forename: A
authors.initials: A
authors.lastname: Michelotti
authors.forename: G
authors.initials: G
authors.lastname: Zavattini
authors.forename: S
authors.initials: S
authors.lastname: Finzi
authors.forename: C
authors.initials: C
authors.lastname: Ghisalberti
authors.forename: F
authors.initials: F
authors.lastname: Marzatico
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D008593:Menopause; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D013267:Stilbenes; D000077185:Resveratrol; D000111:Acetylcysteine