title: ZnO nanorods/Au hybrid nanocomposites for glucose biosensor.
abstract: ZnO nanorods/Au hybrid nanocomposites (ZnO/Au) with Au nanocrystals growing on the surface of ZnO nanorods were synthesized via a simple and facile hydrothermal route. The prepared ZnO/Au nanocomposites were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) for the morphology study. The composites had a good electron transferring and biocompatibility. The glucose biosensor was fabricated by entrapping glucose oxidase (GOx) in this composite matrix using cross-linking method with glutaraldehyde and Nafion solutions. The proposed biosensor responded to glucose linearly over concentration range of 0.1-33.0 μM (R(2)=0.9956), and the detection limit was 10nM (S/N=3) at an operating potential of +0.55 V in pH 7.4 phosphate buffered solution (PBS). The biosensor exhibited a high and reproducible sensitivity, short response time (within 5s), good storage stability and high affinity to GOx (K(M)(app)=0.41 mM). The effects of electroactive interferents at the testing conditions can be negligible which showed a good selectivity of the biosensor. It is estimated that this ZnO/Au is an attractive material for the fabrication of efficient amperometric biosensors.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, China.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wei
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Liu
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide