[Influence of fusion protein of IBDV VP2 and chicken interleukin-2 on immune response in chicken].
In order to research immunogenicity of the recombinant rVP2-IL-2 fusion protein, we obtained the rVP2-IL-2 fusion protein using Pichia pastoris expression system, and then evaluated its potential to induce immune responses in chicken. The effect was determined in the form of protective anti-IBDV VP2 titers, antibodies (IgG1 and IgG2a), lymphocyte proliferation, the levels of interferon-gamma and interleukin-4 cytokines, and challenge experiment. Antibody titers and proliferation lymphocyte level suggested that the fusion protein could elicit specific humoral immune and cellular immune responses, antibody sub-type results indicated that the rVP2-IL-2 fusion protein induced secretion both of IgG1 and IgG2a. The seem result elicited from cytokines ELISA test, secretion of both of Th1 (gamma-IFN) and Th2 (IL-4) were induced by the rVP2-IL-2 fusion protein. Challenge experiment result shown that chicken immunized the rVP2-IL-2 fusion protein obtained 85% protection. These results confirm that the fusion protein enhances the protection against IBDV through both humoral and cell-mediated immunity, and thus could serve as a candidate for the development of IBDV subunit vaccine.
Wang
Chen
C
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Zhao
Zhanqin
Z
Zhang
Chunjie
C
Liu
Yichen
Y
Ding
Ke
K
Li
Yinju
Y
Cheng
Xiangchao
X
Chen
Puyan
P
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D002645:Chickens / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D015678:Viral Structural Proteins / Q000096:biosynthesis* / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; C082513:VP2 protein, infectious bursal disease virus; D022223:Vaccines, Subunit; D015678:Viral Structural Proteins; D014765:Viral Vaccines