title: Evaluation of negative fixed-charge density in tissue-engineered cartilage by quantitative MRI and relationship with biomechanical properties.
abstract: Applying tissue-engineered cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of the cartilage. Magnetic resonance imaging (MRI) of articular cartilage has been widely accepted and applied clinically in recent years. In this study, we evaluated the negative fixed-charge density (nFCD) of tissue-engineered cartilage using gadolinium-enhanced MRI and determined the relationship between nFCD and biomechanical properties. To reconstruct cartilage tissue, articular chondrocytes from bovine humeral heads were embedded in agarose gel and cultured in vitro for up to 4 weeks. The nFCD of the cartilage was determined using the MRI gadolinium exclusion method. The equilibrium modulus was determined using a compressive stress relaxation test, and the dynamic modulus was determined by a dynamic compression test. The equilibrium compressive modulus and dynamic modulus of the tissue-engineered cartilage increased with an increase in culture time. The nFCD value--as determined with the [Gd-DTPA(2-)] measurement using the MRI technique--increased with culture time. In the regression analysis, nFCD showed significant correlations with equilibrium compressive modulus and dynamic modulus. From these results, gadolinium-enhanced MRI measurements can serve as a useful predictor of the biomechanical properties of tissue-engineered cartilage.
authors.affiliation: Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan. miyata@mech.keio.ac.jp
authors.forename: Shogo
authors.initials: S
authors.lastname: Miyata
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Homma
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Numano
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tateishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
mesh_terms: D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D002358:Cartilage, Articular / Q000502:physiology*; D002417:Cattle; D019902:Chondrocytes; D019786:Gadolinium DTPA; D006811:Humerus; D008279:Magnetic Resonance Imaging / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D019786:Gadolinium DTPA