A colorimetric and ratiometric fluorescent probe for thiols and its bioimaging applications.
A naphthalimide-based colorimetric fluorescent probe containing a disulfide group was designed and synthesized, which could detect the physiological level of GSH quantitatively by a ratiometric fluorescence method and was successfully applied to the imaging of thiols in living HeLa cells.
Zhu
Baocun
B
Department of Chemistry, School of Science, Beijing Institute of Technology, Beijing 100081, PR China.
Zhang
Xiaoling
X
Li
Yamin
Y
Wang
Pengfei
P
Zhang
Hongyan
H
Zhuang
Xiaoqing
X
D003124:Colorimetry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D053644:Naphthalimides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D005456:Fluorescent Dyes; D053644:Naphthalimides; D013438:Sulfhydryl Compounds; D005978:Glutathione