title: Rational improvement of cell-free protein synthesis.
abstract: Experimental design principles were applied on cell-free protein synthesis to optimize performance with regard to the expression yield and the incorporation efficiency of amino acid precursors. A versatile screening platform based on batch-mode cell-free expression and central composite design was used. The performance of different extracts (S12 and S30), the concentration dependence of key components and the effect of different additives were investigated. We find that the initial expression yield can be enhanced twofold to threefold in this manner. The improved conditions comprise a modified S12 extract, optimized concentrations of creatine phosphate and key amino acids, as well as introduction of ketoacid additives. Our results show that current cell-free expression technology is far from optimal and that higher yields and increased utilization of the provided precursors are attainable with further optimization.
authors.affiliation: Swedish NMR Centre, University of Gothenburg, PO Box 465, SE-40530 Gothenburg, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Pedersen
authors.forename: Kristofer
authors.initials: K
authors.lastname: Hellberg
authors.forename: Johan
authors.initials: J
authors.lastname: Enberg
authors.forename: B Göran
authors.initials: BG
authors.lastname: Karlsson
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002474:Cell-Free System; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis
chemical_list: D000596:Amino Acids