title: Strategies for three-dimensional particle tracking with holographic video microscopy.
abstract: The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties.
authors.affiliation: Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA.
authors.forename: Fook Chiong
authors.initials: FC
authors.lastname: Cheong
authors.forename: Bhaskar Jyoti
authors.initials: BJ
authors.lastname: Krishnatreya
authors.forename: David G
authors.initials: DG
authors.lastname: Grier
mesh_terms: D000465:Algorithms; D006696:Holography / Q000295:instrumentation* / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D053758:Nanoparticles; D012542:Scattering, Radiation