title: Measurement of N-acetylaspartylglutamate in the human frontal brain by 1H-MRS at 7 T.
abstract: N-Acetylaspartylglutamate in human brain has been measured with difference editing at 7 T. The CH(2) proton resonances (∼ 2.5 ppm) of the aspartyl groups of N-acetylaspartylglutamate and N-acetylaspartate were difference edited (MEGA) using 20-msec gaussian radiofrequency pulses for selective 180 ° rotations of the coupling partners at 4.61 and 4.38 ppm, respectively. The echo time of the editing sequence, 108 msec, was obtained in phantom tests. Single-voxel localized in vivo measurements were conducted in the medial prefrontal and right frontal cortices of five healthy volunteers. The gray and white matter fractions within the voxels were obtained from T(1)-weighted image segmentation. Using linear regression of the metabolite concentration vs. fractional white matter contents within the voxels, the N-acetylaspartylglutamate-to-N-acetylaspartate concentration ratios in gray and white matter were estimated to be 0.13 and 0.28 by difference editing (95% confidence intervals 0.07-0.19 and 0.22-0.34), respectively, assuming identical relaxation effects between the metabolites.
authors.affiliation: Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. changho.choi@utsouthwestern.edu
authors.forename: Changho
authors.initials: C
authors.lastname: Choi
authors.forename: Subroto
authors.initials: S
authors.lastname: Ghose
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Uh
authors.forename: Aditya
authors.initials: A
authors.lastname: Patel
authors.forename: Ivan E
authors.initials: IE
authors.lastname: Dimitrov
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Lu
authors.forename: Deborah
authors.initials: D
authors.lastname: Douglas
authors.forename: Sandeep
authors.initials: S
authors.lastname: Ganji
mesh_terms: D000328:Adult; D000465:Algorithms; D004151:Dipeptides / Q000032:analysis*; D005260:Female; D005625:Frontal Lobe / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011522:Protons
chemical_list: D004151:Dipeptides; D011522:Protons; C027172:isospaglumic acid