Combination of cytomegalovirus enhancer with human cellular promoters for gene-induced chondrogenesis of human bone marrow mesenchymal stem cells.
High-expression plasmid vectors for human mesenchymal stem cells (MSCs) were constructed by combination of cytomegalovirus immediate-early enhancer with cellular promoters. MSCs transfected with the vector showed higher transgene production of a cytokine, which increased the differentiation level to chondrocytes.
Iwai
Ryosuke
R
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
Kumagai
Yoko
Y
Fujiwara
Masashi
M
Wakitani
Shigeyuki
S
Takagi
Mutsumi
M
D001483:Base Sequence; D020219:Chondrogenesis / Q000235:genetics* / Q000502:physiology; D003094:Collagen / Q000235:genetics; D024042:Collagen Type I; D003587:Cytomegalovirus / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004742:Enhancer Elements, Genetic; D017781:Genes, Immediate-Early; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D053782:Transforming Growth Factor beta3 / Q000096:biosynthesis / Q000235:genetics
D024042:Collagen Type I; D017931:DNA Primers; D011994:Recombinant Proteins; D053782:Transforming Growth Factor beta3; D003094:Collagen