Visualization of kinase activity with FRET-based activity biosensors.
Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy.
Depry
Charlene
C
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Zhang
Jin
J
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D005057:Eukaryotic Cells / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008322:Mammals; D008856:Microscopy, Fluorescence; D010770:Phosphotransferases / Q000032:analysis*
D010770:Phosphotransferases