Composite nanofiber mats consisting of hydroxyapatite and titania for biomedical applications.
Composite nanofiber mats (HA/TiO2) consisting of hydroxyapatite (HA) and titania (TiO2) were fabricated via an electrospinning technique and then collagen (type I) was immobilized on the surface of the HA/TiO2 composite nanofiber mat to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite nanofiber mat (HA/TiO2-col) was investigated using an X-ray diffractometer, electron spectroscopy for chemical analysis, and scanning electron microscope. The potential of the HA/TiO2-col composite nanofiber mat for use as a bone scaffold was assessed by an experiment with osteoblastic cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the HA/TiO2-col composite nanofiber mats possess better cell adhesion and significantly higher proliferation and differentiation than untreated HA/TiO2 composite nanofiber mats. This result suggests that the HA/TiO2-col composite nanofiber mat has a high-potential for use in the field of bone regeneration and tissue engineering.
Kim
Hong Mi
HM
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, South Korea.
Chae
Won-Pyo
WP
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, South Korea.
Chang
Ki-Whan
KW
Department of Dental Technology, Daegu Health College, Daegu 702-722, South Korea.
Chun
Sungsu
S
School of Materials Science and Engineering, Yeungnam University, Gyongbuk, South Korea.
Kim
Sukyoung
S
School of Materials Science and Engineering, Yeungnam University, Gyongbuk, South Korea.
Jeong
Yongsoo
Y
Materials Processing Division, Korea Institute of Materials Science, Changwon 641-010, South Korea.
Kang
Inn-Kyu
IK
Department of Polymer Science and Engineering, Kyungpook National University, Daegu 702-701, South Korea.
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002468:Cell Physiological Phenomena / Q000187:drug effects; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D024042:Collagen Type I / Q000494:pharmacology*; D017886:Durapatite; D055767:Immobilized Proteins / Q000494:pharmacology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D055767:Immobilized Proteins; C009495:titanium dioxide; D017886:Durapatite; D014025:Titanium