title: An evaluation of transmitral and pulmonary venous Doppler indices for assessing murine left ventricular diastolic function.
abstract: With the continued development of genetically engineered mouse models of cardiac disease, further advancement of noninvasive techniques for evaluating cardiac diastolic dysfunction in these models would be valuable. Therefore, we performed comprehensive transmitral and pulmonary venous Doppler echocardiographic studies to devise novel indices of diastolic function in a mouse model with cardiac hypertrophy, which were validated against invasively measured hemodynamic parameters. We examined 10 HopX(Tg) transgenic mice with diastolic dysfunction and 10 age-matched controls sedated with 1% to 2% isoflurane (male, age 14-18 weeks). These studies revealed that the acceleration time of the transmitral Doppler E-wave was the best Doppler parameter for unmasking LV diastolic dysfunction in HopX(Tg) mice. This is the first study to assess the utility of the acceleration time of the E-wave and pulmonary venous Doppler echocardiography as a primary diagnostic modality for assessing murine diastolic function.
authors.affiliation: Penn Cardiovascular Institute and Division of Cardiovascular Diseases, University of Pennsylvania, PA 19104, USA.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yuan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Ethan D
authors.initials: ED
authors.lastname: Cohen
authors.forename: Vickas V
authors.initials: VV
authors.lastname: Patel
mesh_terms: D000465:Algorithms; D000818:Animals; D004195:Disease Models, Animal; D015150:Echocardiography, Doppler / Q000379:methods*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D008943:Mitral Valve / Q000000981:diagnostic imaging*; D011667:Pulmonary Veins / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging*