Phylogeographic separation of marine and soil myxobacteria at high levels of classification.
Microorganisms are globally dispersed and are able to proliferate in any habitat that supports their lifestyles, which, however, has not yet been explored in any specific microbial taxon. The social myxobacteria are considered typical soil bacteria because they have been identified in various terrestrial samples, a few in coastal areas, but none in other oceanic environments. To explore the prevalence of marine myxobacteria and to investigate their phylogenetic relationships with their terrestrial counterparts, we established myxobacteria-enriched libraries of 16S rRNA gene sequences from four deep-sea sediments collected at depths from 853 to 4675 m and a hydrothermal vent at a depth of 204 m. In all, 68 different myxobacteria-related sequences were identified from randomly sequenced clones of the libraries of different samples. These myxobacterial sequences were diverse but phylogenetically similar at different locations and depths. However, they were separated from terrestrial myxobacteria at high levels of classification. This discovery indicates that the marine myxobacteria are phylogeographically separated from their terrestrial relatives, likely because of geographic separation and environment selection.
Jiang
De-Ming
DM
Shandong University, Jinan, PR China.
Kato
Chiaki
C
Zhou
Xiu-Wen
XW
Wu
Zhi-Hong
ZH
Sato
Takako
T
Li
Yue-Zhong
YZ
D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology; D009231:Myxococcales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009792:Oceans and Seas; D010802:Phylogeny; D058974:Phylogeography; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012988:Soil Microbiology; D014871:Water Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S