An engineered riboswitch as a potential gene-regulatory platform for reducing antibacterial drug resistance.
Synthetic riboswitch containing aptamer is constructed in E. coli to regulate the expression of β-lactamase through small molecule-aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.
Feng
Xuli
X
Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China.
Liu
Libing
L
Duan
Xinrui
X
Wang
Shu
S
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000494:pharmacology*; D020134:Catalytic Domain / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D015394:Molecular Structure; D058928:Riboswitch / Q000187:drug effects; D013806:Theophylline / Q000737:chemistry / Q000494:pharmacology; D018440:beta-Lactam Resistance / Q000187:drug effects* / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D058928:Riboswitch; D013806:Theophylline; D001618:beta-Lactamases