title: Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae).
abstract: Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1(T) and FFA3(T), and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1(T)) and 99.35 % (FFA3(T)) to Ignatzschineria larvae L1/68(T). The level of gene sequence similarity between strains FFA1(T) and FFA3(T) was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1(T) and FFA3(T) shared 24 % DNA-DNA relatedness. DNA-DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1(T) and 44 % for strain FFA3(T)) and I. larvae L1/68(T) genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1(T) and FFA3(T), respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C(16 : 0), summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c), CyC(19 : 0)ω8c and C(14 : 0). The results of DNA-DNA hybridization between the two new strains and I. larvae L1/68(T), in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1(T) and FFA3(T) represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1(T) = DSM 22309(T) = KCTC 22643(T) = NCIM 5325(T)) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3(T) = DSM 22310(T) = KCTC 22644(T) = NCIM 5326(T)) are proposed.
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Gupta
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Mahesh Shantappa
authors.initials: MS
authors.lastname: Dharne
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Ashraf Yusuf
authors.initials: AY
authors.lastname: Rangrez
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Verma
authors.affiliation: Department of Zoology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune 411004, Maharashtra, India.
authors.forename: Hemant V
authors.initials: HV
authors.lastname: Ghate
authors.affiliation: Helmholtz-Zentrum für Infektionsforschung GmbH, D-38124 Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Milind Shivaji
authors.initials: MS
authors.lastname: Patole
authors.affiliation: Microbial Culture Collection, Hindustan Antibiotics Ltd. Complex, Pimpri, Pune 411018, Maharashtra, India.|Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
authors.forename: Yogesh Shreepad
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058493:Sarcophagidae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase