[Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells].
OBJECTIVE
To establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells (BMSC-SMCs) in vitro and explore the relationship between scavenger receptors A (SR-A) and caveolin-1.


METHODS
BMSCs were isolated from the femoral bone of SD rats by adherent culture. After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h, Western blotting was performed to detect the expression of scavenger receptor SR-A, cell cholesterol transport protein ATP-binding cassette transporter Al (ABCA1) and caveolin-1.


RESULTS
BMCS-SMCs became foam cells after treatment with ox-LDL. BMSC-SMC gave rise to more foam cell formation than VSMCs did. Western blotting showed that treatment with 80 mg/L ox-LDL for 72 h resulted in significantly increased expression of SR-A and significantly decreased expressions of ABCA1 and caveolin-1.


CONCLUSIONS
Treatment of BMCS-SMCs with ox-LDL results in cholesterol ester accumulation in the cells to result in foam cells, the mechanism of which involves up-regulation of scavenger receptor SR-A expression and down-regulation of the reverse cholesterol transport protein ABCA1 and caveolin-1 expression.
Yan
Peng-ke
PK
The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, China. Yanpk@tom.com
Li
Shi-huang
SH
Duang
Cai-wen
CW
Mei
Zheng-Rong
ZR
Tan
Xiang-ping
XP
Si
Tu-bing
TB
Xiao
Guo-hong
GH
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D051242:Caveolin 1 / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005487:Foam Cells / Q000166:cytology*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051117:Scavenger Receptors, Class A / Q000378:metabolism*
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; C495537:Cav1 protein, rat; D051242:Caveolin 1; D008077:Lipoproteins, LDL; D051117:Scavenger Receptors, Class A; C099252:oxidized low density lipoprotein