title: Genomic analysis of porcine circovirus type-2 isolates in Alberta pigs demonstrating clinical porcine circovirus associated disease (PCVAD).
abstract: Nineteen pigs with clinical signs of porcine circovirus associated diseases (PCVAD) on 5 Alberta pig farms were examined pathologically, including gross pathology, histopathology, and immunohistochemistry. Polymerase chain reaction (PCR) for porcine circovirus type-2 (PCV-2) and sequence analysis was performed on tissue samples of 12 animals. Results showed that new strains of porcine circovirus type-2 genogroup b were present in most pigs that were positive for PCV-2. Furthermore, a mixed infection with PCV-2a and PCV-2b occurred in the liver and lungs of 1 pig. Comparison of whole genome sequences representing known viruses and the newly discovered Alberta viruses revealed mutations distributed over the entire genome of PCV-2; however, sequences encoding for immunodominant epitopes of PCV-2 appear to be unaffected by these mutations.
authors.affiliation: Department of Production Animal Health, University of Calgary, Alberta, Canada.
authors.forename: Leila
authors.initials: L
authors.lastname: McIntyre
authors.forename: Mark
authors.initials: M
authors.lastname: Chaiyakul
authors.forename: Edward G
authors.initials: EG
authors.lastname: Clark
authors.forename: Frank
authors.initials: F
authors.lastname: Marshall
authors.forename: Markus
authors.initials: M
authors.lastname: Czub
mesh_terms: D000416:Alberta / Q000453:epidemiology; D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D016056:Immunodominant Epitopes / Q000235:genetics; D009154:Mutation; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*
chemical_list: D016056:Immunodominant Epitopes