title: Interactions between cationic lipid bilayers and model chromatin.
abstract: Complexes formed in mixtures of cationic liposomes of varying charge density and nucleosome core particles (NCPs) or nucleosome arrays have been characterized. Under most of the conditions studied, the lipids and NCPs or arrays formed lamellar structures similar to those obtained with the liposomes and pure DNA. Thus, the dissociation of DNA from the NCP or nucleosome array and the formation of a DNA-lipid complex is thermodynamically favored, which can likely be ascribed mainly to the gain in entropy on release of the small counterions. Only at very low liposome charge densities are there indications that the NCPs/arrays do not dissociate upon interaction with the lipid bilayers. The reported results can serve as a valuable reference point in investigations of biologically more relevant systems.
authors.affiliation: Department of Chemistry, University of Coimbra, Rua Larga, 3004-535 Coimbra, Portugal.
authors.forename: Dan
authors.initials: D
authors.lastname: Lundberg
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Berezhnoy
authors.forename: Chenning
authors.initials: C
authors.lastname: Lu
authors.forename: Nikolay
authors.initials: N
authors.lastname: Korolev
authors.forename: Chun-Jen
authors.initials: CJ
authors.lastname: Su
authors.forename: Viveka
authors.initials: V
authors.lastname: Alfredsson
authors.forename: Maria da Graça
authors.initials: Mda G
authors.lastname: Miguel
authors.forename: Björn
authors.initials: B
authors.lastname: Lindman
authors.forename: Lars
authors.initials: L
authors.lastname: Nordenskiöld
mesh_terms: D002412:Cations / Q000737:chemistry*; D002843:Chromatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008958:Models, Molecular
chemical_list: D002412:Cations; D002843:Chromatin; D008051:Lipid Bilayers; D004247:DNA