Sauchinone, a lignan from Saururus chinensis, reduces tumor necrosis factor-alpha production through the inhibition of c-raf/MEK1/2/ERK 1/2 pathway activation.
We investigated the anti-inflammatory effects of sauchinone, a lignan isolated from Saururus chinensis, and the underlying mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. To assess the effects of sauchinone on LPS-induced macrophages activation, we measured the production of tumor necrosis factor (TNF)-alpha and macrophage inflammatory protein (MIP)-2, and activation of mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase (ERK) 1/2, c-Jun amino terminal kinases and p38 mitogen-activated protein kinase, and NF-kappaB activation in RAW264.7 cells. Sauchinone decreased the production of TNF-alpha, but not MIP-2 production in RAW264.7 cells stimulated with LPS. Sauchinone also decreased c-Raf-MEK1/2-ERK1/2 phosphorylation and NF-kappaB activation in RAW264.7 cells stimulated with LPS. Our results show that sauchinone inhibits LPS-induced TNF-alpha expression in macrophages by suppression of NF-kappaB activation via ERK1/2 pathway, which may constitute anti-inflammatory effects of sauchinone.
Bae
Hong-Beom
HB
Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 8 Hak-dong, Gwangju 501-746, Korea.
Li
Mei
M
Son
Jong-Keun
JK
Seo
Chang-Seob
CS
Chung
Seung-Hyun
SH
Kim
Seok-Jai
SJ
Jeong
Cheol-Won
CW
Lee
Hyung-Gon
HG
Kim
Woongmo
W
Park
Hwan-Chul
HC
Kwak
Sang-Hyun
SH
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D054426:Chemokine CXCL2 / Q000096:biosynthesis; D004149:Dioxoles / Q000302:isolation & purification / Q000494:pharmacology*; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D019908:Proto-Oncogene Proteins c-raf / Q000037:antagonists & inhibitors*; D032134:Saururaceae / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D004149:Dioxoles; D017705:Lignans; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C410958:sauchinone; D019908:Proto-Oncogene Proteins c-raf; D020928:Mitogen-Activated Protein Kinases