[Application of polyguanidine solution for fixation of biological and anatomical specimens].
A new method for fixation of biological material is described, and its effectiveness is compared to that one of formalin fixation. As an embalming agent, polyhexamethylenguanidine (PHMG) hydrochloride was used. Using the proposed method of fixation, the anatomical and histological preparations of human organs and of chick embryos at developmental 12 days, were produced. The anatomical preparations obtained show the appearance, similar to that of the recently removed organs. Histological preparations were free from significant distortions of the microscopic characteristics of the specimens, which are typical to the material fixed with formalin. The results of the study suggest the possibility of PHMG application in the morphological studies.
Anichkov
N M
NM
Danilova
I A
IA
Riabinin
I A
IA
Kipenko
A V
AV
D000818:Animals; D002642:Chick Embryo; D005404:Fixatives / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives; D006146:Guanidines; C060540:polyhexamethyleneguanidine