title: Comprehensive two-dimensional liquid chromatography: ion chromatography × reversed-phase liquid chromatography for separation of low-molar-mass organic acids.
abstract: In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt.
authors.affiliation: Analytical Chemistry Group (HIMS), University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.
authors.forename: Stella S
authors.initials: SS
authors.lastname: Brudin
authors.forename: Robert A
authors.initials: RA
authors.lastname: Shellie
authors.forename: Paul R
authors.initials: PR
authors.lastname: Haddad
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schoenmakers
mesh_terms: D001628:Beverages / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D008970:Molecular Weight; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis; D015014:Yogurt / Q000032:analysis
chemical_list: D002264:Carboxylic Acids