[Antidysmenorrheic effects of Radix angelica and Rhizoma Chuanxiong with different proportions and preparation methods on dysmenorrhea model mice].
OBJECTIVE
To study the effect of Radix Angelica and Rhizoma Chuanxiong with different proportions (1 : 0, 2 : 1, 1.5 : 1, 1 : 1, 1 : 1.5, 1 : 2, 0 : 1), different extraction in order (by water, by ethanol, firstly by alcohol then by water) on dysmenorrhea model mice in order to observe their compatibility rules and material foundation.


METHODS
The mice model of primary dysmenorrhea was used to study the effect of Radix Angelica and Rhizoma Chuanxiong with different proportions and preparation methods on writhing response and the levels of nitric oxide and calcium ion in mice uterine tissue.


RESULTS
Danggui-Chuanxiong (1. 5 : 1) and solution extracted firstly by alcohol then by water showed the strongest effect on primary dysmenorrhea by reducing the writhing times, increasing nitric oxide (NO) concentration and reducing calcium ion (Ca2+) concentration in uterine tissue.


CONCLUSIONS
Radix Angelica and Rhizoma Chuanxiong with different proportions and preparation methods showed different antidysmenorrheic trend on dysmenorrhea model mice, which was related with the containing constituents.
Wang
Huan
H
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Tang
Yuping
Y
Guo
Jianming
J
Ding
Anwei
A
Li
Weixia
W
Jiang
Wei
W
Duan
Jinao
J
D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D001522:Behavior, Animal / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002626:Chemistry, Pharmaceutical; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D004412:Dysmenorrhea / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005260:Female; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D004365:Drugs, Chinese Herbal; C534193:chuanxiong rhizome preparation; D009569:Nitric Oxide; D002118:Calcium