title: Brazilian experience with the medium-term multi-organ bioassay: scientific and regulatory developments.
abstract: In 1996 the Brazilian Institute for the Environment (IBAMA) officially adopted a variation of the multi-organ initiation-promotion DMBDD bioassay as a valid source of evidence of the carcinogenic potential of pesticides. The protocol adopted by IBAMA was a modification of the one originally proposed by researchers led by Nobuyuki Ito, from the Nagoya City University Medical School. Among the modifications established in the Brazilian protocol were the use of both sexes of the outbreed Wistar strain of rats and two positive control test chemicals. The adoption of the modified DMBDD protocol was instrumental during the last decade for qualifying technical people and to spread knowledge on chemical carcinogenesis in Brazil.
authors.affiliation: São Paulo State University (UNESP) Medical School, Department of Pathology, UNESP Medical School, Botucatu, SP, Brazil. decam@fmb.unesp.br
authors.forename: João Lauro V
authors.initials: JL
authors.lastname: de Camargo
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000592:standards*; D001938:Brazil; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D033161:Government Regulation; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D009928:Organ Specificity / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens