Development of an enzyme-linked immunosorbent assay for the rapid detection of haloxyfop-P-methyl.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the herbicide haloxyfop-P-methyl is reported. Two haptens of haloxyfop-P-methyl with different spacer-arm lengths were synthesized, to which polyclonal antibodies were obtained by immunizing New Zealand rabbits. The most sensitive combination of antibody/coating antigen was selected by homologous and heterologous assays. The results indicated that the spacer-arm length of hapten can affect assay sensitivity. The optimized ic-ELISA showed that the 50% inhibitory concentration (IC(50)) was 0.0419 mg/L, and the limit of detection (LOD) was 0.0019 mg/L. Recoveries were obtained from the agricultural samples, including water, soil, cucumbers, cabbages, tomatoes, and potatoes; all were in the range of 87.4%-111.8%, well within the requirements of residue detection.
Bao
Huajun
H
Department of Pesticide Science, Nanjing Agricultural University, Nanjing, China.
Fang
Song
S
Liu
Zhenjiang
Z
Shi
Haiyan
H
Ye
Yonghao
Y
Wang
Minghua
M
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006540:Herbicides / Q000032:analysis*; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis*; D011725:Pyridines / Q000032:analysis*; D011817:Rabbits; D012989:Soil Pollutants / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
D006540:Herbicides; D010573:Pesticide Residues; D011725:Pyridines; D012989:Soil Pollutants; C054176:haloxyfop