Cellular immune responses in cetaceans immunized with a porcine erysipelas vaccine.
Clinical erysipelas represents a significant health problem in managed cetacean species. Vaccination was suspended in many oceanariums in the past due to losses associated with vaccine-induced hypersensitivities which were deemed to be a greater threat than clinical erysipelas. A perceived shift in clinical presentation of erysipelas from a chronic dermatologic form to an acute systemic form in dolphins sparked interest in re-initiating vaccination with improved subunit vaccines of Erysipelothrix rhusiopathiae. This manuscript describes the development and application of in vitro correlates of immunity (T(H)1, T(H)2 and T(REG)) in Tursiops truncatus induced by immunization with a commercial porcine 65 kDa subunit E. rhusiopathiae vaccine. Variable degrees of pre-existing T cell memory were identified prior to vaccination. Vaccine-induced IFN gamma responses were consistent with a T(H)1 response and associated with elimination of erysipelas in all vaccinated animals. Comparative analysis between six-month and 12-month vaccination booster regimes demonstrated maintenance of superior memory in the six-month group; however, anamnestic responses induced by booster were only identified in the 12-month group. To our knowledge, this is the first study to develop and apply advanced immunologic analyses for assessing vaccine efficacy in captive or free-ranging wildlife.
Sitt
Tatjana
T
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. tsitt@ucdavis.edu
Bowen
Lizabeth
L
Blanchard
Myra T
MT
Gershwin
Laurel J
LJ
Byrne
Barbara A
BA
Dold
Christopher
C
McBain
Jim
J
Stott
Jeffrey L
JL
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D050681:Bottle-Nosed Dolphin / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004888:Erysipelothrix / Q000276:immunology*; D005260:Female; D008297:Male; D013552:Swine; D013554:Swine Erysipelas / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination / Q000662:veterinary*
D001428:Bacterial Vaccines; D016207:Cytokines