Role of glycerol-3-phosphate dehydrogenase 2 in mouse sperm capacitation.
A tyrosine phosphoproteome study of hamster spermatozoa indicated that glycerol-3-phosphate dehydrogenase 2 (GPD2), is one of the proteins that enables tyrosine phosphorylation during sperm capacitation. Further, enzymatic activity of GPD2 correlated positively with sperm capacitation [Kota et al., 2009; Proteomics 9:1809-1826]. Therefore, understanding the function of GPD2 would help to unravel the molecular mechanism of sperm capacitation. In this study, involving the use of spermatozoa from Gpd2(+/+) and Gpd2(-/-) mice, it has been demonstrated that in the absence of Gpd2, hyperactivation and acrosome reaction were significantly altered, and a few changes in protein tyrosine phosphorylation were also observed during capacitation. Evidence is provided to demonstrate that GPD2 activity is required for ROS generation in mouse spermatozoa during capacitation, failing which, capacitation is impaired. These results imply that GPD2 is involved in sperm capacitation.
Kota
Venkatesh
V
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India.
Rai
Priyanka
P
Weitzel
Joachim M
JM
Middendorff
Ralf
R
Bhande
Satish S
SS
Shivaji
Sisinthy
S
D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D006224:Cricetinae; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism / Q000502:physiology; D013075:Sperm Capacitation / Q000235:genetics / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology; D013094:Spermatozoa / Q000201:enzymology / Q000502:physiology
D019000:Phosphotyrosine; D005993:Glycerolphosphate Dehydrogenase