title: Lactobacillus plantarum prevents the development of colitis in IL-10-deficient mouse by reducing the intestinal permeability.
abstract: It is reported that defects exist in the small intestinal epithelial barrier of inflammatory bowel disease, which might be associated with increased intestinal permeability at a very early stage. Our study aims to investigate the role of Lactobacillus plantarum on the decrease of epithelial permeability and the further protective effects on the intestinal epithelial barrier using the IL-10-deficient mouse model. Our study showed that tight junction associated proteins were increased after the pre-treatment of L. plantarum by fluorescence staining, western blot, real-time PCR and transmission electron microscope. Oral gavage of milk containing L. plantarum was effective in decreasing small intestinal permeability using methods of Ussing chamber assay and sugar probe. Assay of pro-inflammatory cytokines IFN-γ, TNF-α, MPO, and colonic histology by ELISA showed protective effects of L. plantarum on the intestinal epithelial barrier. Therefore, L. plantarum may prevent the development of colitis in IL-10-deficient mice by blocking changes in the expression of TJ proteins, TJ structure and intestinal permeability.
authors.affiliation: Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, 200233, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Ma
authors.forename: Hongqi
authors.initials: H
authors.lastname: Chen
authors.forename: Yukun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Chu
authors.forename: Huanlong
authors.initials: H
authors.lastname: Qin
mesh_terms: D000818:Animals; D003092:Colitis / Q000382:microbiology* / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D007249:Inflammation; D016753:Interleukin-10 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk; D010539:Permeability; D009195:Peroxidase / Q000378:metabolism; D019108:Tight Junctions
chemical_list: D016207:Cytokines; D016753:Interleukin-10; D009195:Peroxidase