Importance of dynamic culture for evaluating osteoblast activity on dense silicon-substituted hydroxyapatite.
This paper reports an investigation on human osteoblast-like cells (SaOs-2) seeded onto pure hydroxyapatite (HA) and silicon-substituted HA (SiHA) tablets under static and dynamic culture conditions. The biological characterizations were conducted in classical static conditions in multi-wells plates, and in a perfusion bioreactor that permits continuous circulation of culture medium at 2 mL/h. The morphology, proliferation and differentiation of osteoblastic cells were examined for the two types of samples in the both culture conditions after 1, 3 and 8 days. Under dynamic conditions, cells cultured on SiHA surfaces showed a faster adhesion process and the formation of longer and thinner focal adhesions than in static conditions. The number of cells grown onto both ceramic surfaces was higher in dynamic conditions when compared with static conditions. Moreover, a higher activity of alkaline phosphatase was found for cells seeded under dynamic conditions. Our findings suggest that the application of perfusion culture system on cells cultured on dense substrates is valuable for predicting in vivo behaviour of cells on biomaterials.
da Silva
H Marques
HM
Dep de Eng Metal e de Materiais, COPPE/UFRJ, CP 68505, 21941-972 Rio de Janeiro, Brazil.
Mateescu
M
M
Damia
C
C
Champion
E
E
Soares
G
G
Anselme
K
K
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*
D001672:Biocompatible Materials; D017886:Durapatite; D000469:Alkaline Phosphatase; D012825:Silicon