The effect of tumor-associated protein RCAS1 gene silencing on blood pressure and urinary protein excretion in pregnant mouse: a pilot study.
OBJECTIVE
The level of tumor-associated receptor-binding cancer antigen that is expressed on SiSo cells (RCAS1) is decreased significantly in preeclamptic pregnancies. We hypothesized that RCAS1 protein gene silencing might affect blood pressure and proteinuria in pregnant mice.


METHODS
On postcoital day 7.5, pregnant imprinting control region mice were subjected to the transfer of small interfering RNA (siRNA) against RCAS1 protein into the uterine cavity with the use of a hemagglutinating virus Japan envelope. Scramble siRNA was used as a negative control. Blood pressure and urine albumin/creatinine measurements were performed. The effect of the transferred siRNA was examined in uterine samples on postcoital day 8.5 with the use of Western blotting and immunohistochemistry analyses.


RESULTS
In the RCAS1 siRNA group, blood pressure significantly raised on postcoital days 9.5, 10.5, 11.5, and 15.5, whereas urine albumin/creatinine ratio was significantly increased on postcoital day 9.5


CONCLUSIONS
Our results suggest the importance of RCAS1 protein in the pathophysiologic condition of preeclampsia.
Tskitishvili
Ekaterine
E
Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan. ekaterinet@hotmail.com
Nakamura
Hitomi
H
Kinugasa-Taniguchi
Yukiko
Y
Kanagawa
Takeshi
T
Shimoya
Koichiro
K
Tomimatsu
Takuji
T
Kimura
Tadashi
T
D000419:Albuminuria; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001794:Blood Pressure; D015153:Blotting, Western; D003404:Creatinine / Q000652:urine*; D005260:Female; D020868:Gene Silencing; D007150:Immunohistochemistry; D051379:Mice; D010865:Pilot Projects; D011225:Pre-Eclampsia / Q000235:genetics; D011247:Pregnancy; D011256:Pregnancy Outcome; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
D000951:Antigens, Neoplasm; C534295:EBAG9 protein, mouse; D034741:RNA, Small Interfering; D003404:Creatinine