title: Proteomic analysis of field cancerization in pharynx and oesophagus: a prospective pilot study.
abstract: 'Field cancerization' in head and neck squamous cell carcinoma (HNSCC) is poorly understood and it may extend from the pharynx into the oesophagus. Both local recurrences and second primary carcinomas/second field tumours may originate from field cancerization. Our prospective pilot study aimed at the identification of patients suffering from field cancerization on the basis of mucosal protein profiles. Five mucosal biopsies from the oropharynx, hypopharynx and from three regions of the oesophagus were taken from 24 patients. Protein profiles were generated from the mucosal biopsies. After classifier learning, using the profiles of the patients without tumour diagnosis (n = 9), we were able to discriminate between the different mucosal sites and between healthy mucosa and HNSCC using tumour and healthy tissue samples. Mucosal biopsies of tumour patients (n = 15) revealed changes in the protein profiles similar to those in the tumours. During 42 months median follow-up, six tumour patients experienced local recurrences and second field tumours, of which three occurred in the oesophagus. In all six cases, tumour relapse was correctly predicted by altered mucosal protein profiles (p = 0.007, Fisher's exact test, two-tailed). Consequently, molecular field cancerization had a strong impact on progression-free survival (p = 0.007, log-rank test). Protein profiles of small diagnostic biopsies hold great promise to improve personalized risk assessment in HNSCC. Larger studies are needed to further substantiate these findings.
authors.affiliation: Molecular Biology Laboratory, Department of Otolaryngology, Head and Neck Surgery, University of Heidelberg, INF 400, Heidelberg, Germany.
authors.forename: Mariana
authors.initials: M
authors.lastname: Roesch-Ely
authors.forename: Alexandra
authors.initials: A
authors.lastname: Leipold
authors.forename: Matthias
authors.initials: M
authors.lastname: Nees
authors.forename: Dana
authors.initials: D
authors.lastname: Holzinger
authors.forename: Andreas
authors.initials: A
authors.lastname: Dietz
authors.forename: Christa
authors.initials: C
authors.lastname: Flechtenmacher
authors.forename: Thomas
authors.initials: T
authors.lastname: Wolf
authors.forename: Marc
authors.initials: M
authors.lastname: Zapatka
authors.forename: Franz X
authors.initials: FX
authors.lastname: Bosch
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D004812:Epidemiologic Methods; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009092:Mucous Membrane / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010610:Pharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins