Cerebralcare Granule, a Chinese herb compound preparation, improves cerebral microcirculatory disorder and hippocampal CA1 neuron injury in gerbils after ischemia-reperfusion.
OBJECTIVE
Cerebralcare Granule (CG) is a Chinese herb compound preparation that has been used for treatment of cerebrovascular related diseases. However, the effect of post-treatment with CG on ischemia and reperfusion (I/R) induced cerebral injury is so far unclear.


METHODS
In present study, cerebral global I/R was induced in Mongolian gerbils by clamping bilateral carotid arteries for 30 min followed by reperfusion for 5 days, and CG (0.4 g/kg or 0.8 g/kg) was administrated 3h after the initiation of reperfusion.


RESULTS
Post-treatment with CG for 5 days attenuated the I/R-induced production of hydrogen peroxide in, leukocyte adhesion to, and albumin leakage from cerebral microvessels, and, meanwhile, protected neuron from death, reduced the number of caspase-3- and Bax-positive cells, and increased Bcl-2-positive cells in hippocampal CA1 region.


CONCLUSIONS
The results suggest that CG given after initiation of reperfusion is able to ameliorate cerebral microvascular dysfunction and hippocampal CA1 neuron damage caused by I/R.
Sun
Kai
K
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.
Hu
Qin
Q
Zhou
Chang-Man
CM
Xu
Xiang-Shun
XS
Wang
Fang
F
Hu
Bai-He
BH
Zhao
Xin-Yong
XY
Chang
Xin
X
Chen
Chun-Hua
CH
Huang
Ping
P
An
Li-Hua
LH
Liu
Yu-Ying
YY
Fan
Jing-Yu
JY
Wang
Chuan-She
CS
Yang
Lei
L
Han
Jing-Yan
JY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002199:Capillary Permeability; D002317:Cardiovascular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002550:Cerebral Veins / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005849:Gerbillinae; D006861:Hydrogen Peroxide / Q000378:metabolism; D036904:Leukocyte Rolling / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D012709:Serum Albumin / Q000378:metabolism; D014699:Venules / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D012709:Serum Albumin; D051028:bcl-2-Associated X Protein; C553222:cerebralcare preparation; D006861:Hydrogen Peroxide; D053148:Caspase 3