Production of polyclonal antibodies against the tegument of sparganum (plerocercoid of Spirometra mansoni) and its immunolocalization.
In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.
Yang
Hyun-Jong
HJ
Department of Parasitology, Ewha Womans University, School of Medicine, Seoul 158-710, Korea. parayang@ewha.ac.kr
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D008970:Molecular Weight; D013146:Spirometra / Q000033:anatomy & histology / Q000737:chemistry* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth