Protective effects of flavonoids against oxidative stress induced by simulated microgravity in SH-SY5Y cells.
Many lines of evidence suggest that microgravity results in increased oxidative stress in the nervous system. In order to protect neuronal cells from oxidative damage induced by microgravity, we selected some flavonoids that might prevent oxidative stress because of their antioxidant activities. Among the 20 flavonoids we examined, we found that isorhamnetin and luteolin had the best protective effects against H(2)O(2) or SIN-1-induced cytotoxicity in SH-SY5Y cells. Using a clinostat to simulate microgravity, we found that isorhamnetin and luteolin treatment protected SH-SY5Y cells by preventing microgravity-induced increases in reactive oxygen species (ROS), nitric oxide (NO) and 3-nitrotyrosine (3-NT) levels, and a decrease in antioxidant power (AP). Moreover, isorhamnetin and luteolin treatment downregulated the expression of inducible nitric oxide synthase (iNOS), and oxidative stress was significantly inhibited by an iNOS inhibitor in SH-SY5Y cells exposed to simulated microgravity (SMG). These results indicate that isorhamnetin and luteolin could protect against microgravity-induced oxidative stress in neuroblastoma SH-SY5Y cells by inhibiting the ROS-NO pathway. These two flavonoids may have potential for preventing oxidative stress induced by space flight or microgravity.
Qu
Lina
L
Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, Beijing, China.
Chen
Hailong
H
Liu
Xinmin
X
Bi
Lei
L
Xiong
Jianghui
J
Mao
Zebin
Z
Li
Yinghui
Y
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047311:Luteolin / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000031:analogs & derivatives; D017382:Reactive Oxygen Species / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014893:Weightlessness / Q000009:adverse effects*
D000975:Antioxidants; D044948:Flavonols; D020030:Nitric Oxide Donors; D016877:Oxidants; D017382:Reactive Oxygen Species; C047368:3-methylquercetin; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; C002385:linsidomine; D011794:Quercetin; D006861:Hydrogen Peroxide; D008981:Molsidomine; D052247:Nitric Oxide Synthase Type II; D047311:Luteolin