Morphological characterization of periodontium-derived human stem cells.
The aim of this study has been to characterize adult human somatic periodontium-derived stem cells (PDSCS) isolated from human periodontium and to follow their differentiation after cell culture. PDSCS were isolated from human periodontal tissue and cultured as spheres in serum-free medium. After 10 days the primary spheres were dissociated and the secondary spheres sub-cultured for another 1-2 weeks. Cells from different time points were analyzed, and immunohistochemical and electron microscopic investigations carried out. Histological analysis showed differentiation of spheres deriving from the PDSCS with central production of extracellular matrix beginning 3 days after sub-culturing. Isolated PDSCS developed pseudopodia which contained actin. Tubulin was found in the central portion of the cells. Pseudopodia between different cells anastomosed, indicating intercellular transport. Immunostaining for osteopontin demonstrated a positive reaction in primary spheres and within extracellular matrix vesicles after sub-culturing. In cell culture under serum-free conditions human PDSCS form spheres which are capable of producing extracellular matrix. Further investigations have do be carried out to investigate the capability of these cells to differentiate into osteogenic progenitor cells.
Arnold
Wolfgang H
WH
University of Witten/Herdecke, Germany. wolfgang.arnold@uni-wh.de
Becher
Sebastian
S
Dannan
A
A
Widera
Darius
D
Dittmar
T
T
Jacob
Monica
M
Mannherz
Hans Georg
HG
Dittmar
Thomas
T
Kaltschmidt
B
B
Kaltschmidt
C
C
Grimm
Wolf-Dieter
WD
D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D048430:Cell Shape; D022081:Cell Surface Extensions / Q000648:ultrastructure; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D053495:Osteopontin / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology*; D011554:Pseudopodia / Q000648:ultrastructure
D016895:Culture Media, Serum-Free; D053495:Osteopontin