title: Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen.
abstract: A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Parima
authors.initials: P
authors.lastname: Boontanom
authors.forename: Suradej
authors.initials: S
authors.lastname: Siripattanapipong
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
authors.forename: Peerapan
authors.initials: P
authors.lastname: Tan-ariya
authors.forename: Saovanee
authors.initials: S
authors.lastname: Leelayoova
mesh_terms: D017931:DNA Primers; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013785:Thailand
chemical_list: D017931:DNA Primers; D005969:Glutamate Dehydrogenase