title: Electrophysiological studies of transgenic long QT type 1 and type 2 rabbits reveal genotype-specific differences in ventricular refractoriness and His conduction.
abstract: We have generated transgenic rabbits lacking cardiac slow delayed-rectifier K(+) current [I(Ks); long QT syndrome type 1 (LQT1)] or rapidly activating delayed-rectifier K(+) current [I(Kr); long QT syndrome type 2 (LQT2)]. Rabbits with either genotype have prolonged action potential duration and QT intervals; however, only LQT2 rabbits develop atrioventricular (AV) blocks and polymorphic ventricular tachycardia. We therefore sought to characterize the genotype-specific differences in AV conduction and ventricular refractoriness in LQT1 and LQT2 rabbits. We carried out in vivo electrophysiological studies in LQT1, LQT2, and littermate control (LMC) rabbits at baseline, during isoproterenol infusion, and after a bolus of dofetilide and ex vivo optical mapping studies of the AV node/His-region at baseline and during dofetilide perfusion. Under isoflurane anesthesia, LQT2 rabbits developed infra-His blocks, decremental His conduction, and prolongation of the Wenckebach cycle length. In LQT1 rabbits, dofetilide altered the His morphology and slowed His conduction, resulting in intra-His block, and additionally prolonged the ventricular refractoriness, leading to pseudo-AV block. The ventricular effective refractory period (VERP) in right ventricular apex and base was significantly longer in LQT2 than LQT1 (P < 0.05) or LMC (P < 0.01), with a greater VERP dispersion in LQT2 than LQT1 rabbits. Isoproterenol reduced the VERP dispersion in LQT2 rabbits by shortening the VERP in the base more than in the apex but had no effect on VERP in LQT1. EPS and optical mapping experiments demonstrated genotype-specific differences in AV conduction and ventricular refractoriness. The occurrence of infra-His blocks in LQT2 rabbits under isoflurane and intra-His block in LQT1 rabbits after dofetilide suggest differential regional sensitivities of the rabbit His-Purkinje system to drugs blocking I(Kr) and I(Ks).
authors.affiliation: Cardiovascular Research Center, Division of Cardiology, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA.
authors.forename: Katja E
authors.initials: KE
authors.lastname: Odening
authors.forename: Malcolm
authors.initials: M
authors.lastname: Kirk
authors.forename: Michael
authors.initials: M
authors.lastname: Brunner
authors.forename: Ohad
authors.initials: O
authors.lastname: Ziv
authors.forename: Peem
authors.initials: P
authors.lastname: Lorvidhaya
authors.forename: Gong Xin
authors.initials: GX
authors.lastname: Liu
authors.forename: Lorraine
authors.initials: L
authors.lastname: Schofield
authors.forename: Leonard
authors.initials: L
authors.lastname: Chaves
authors.forename: Xuwen
authors.initials: X
authors.lastname: Peng
authors.forename: Manfred
authors.initials: M
authors.lastname: Zehender
authors.forename: Bum-Rak
authors.initials: BR
authors.lastname: Choi
authors.forename: Gideon
authors.initials: G
authors.lastname: Koren
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D054537:Atrioventricular Block / Q000235:genetics / Q000503:physiopathology; D001283:Atrioventricular Node / Q000187:drug effects / Q000503:physiopathology*; D002036:Bundle of His / Q000187:drug effects / Q000503:physiopathology*; D002316:Cardiotonic Agents / Q000494:pharmacology; D004594:Electrophysiology; D005838:Genotype; D007545:Isoproterenol / Q000494:pharmacology; D008133:Long QT Syndrome / Q000235:genetics* / Q000503:physiopathology; D011817:Rabbits
chemical_list: D002316:Cardiotonic Agents; D007545:Isoproterenol