title: Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar.
abstract: OBJECTIVE
  Orthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.
  
  
  METHODS
  Tiny fragments of surgical tumors were implanted into the mice brain with a trocar system. Immunohistochemistry was performed to detect brain tumor stem cells among glioblastoma tissues, including both the original and resulting ones with monoclonal antibody against CD133.
  
  
  RESULTS
  Besides the constant high take rates in both models; brain transplants perfectly resembled their original tumors in biological behaviors. The brain tumor stem cells, positively stained with CD133 were found, though not frequently, in both original and resulting glioblastoma tissues.
  
  
  CONCLUSIONS
  Orthotopic model established with a trocar system is effective and injection of tumor tissues containing stem cells promise the forming of new tumor mass when grafted.
authors.affiliation: Neurosurgical Department, Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, 215006, Suzhou, China.
authors.forename: Xi Feng
authors.initials: XF
authors.lastname: Fei
authors.forename: Quan Bin
authors.initials: QB
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.forename: Yi
authors.initials: Y
authors.lastname: Diao
authors.forename: Zhi Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Ru Jun
authors.initials: RJ
authors.lastname: Li
authors.forename: Zi Cheng
authors.initials: ZC
authors.lastname: Wu
authors.forename: Ai Dong
authors.initials: AD
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.forename: Shi Ming
authors.initials: SM
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001932:Brain Neoplasms / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D005909:Glioblastoma / Q000473:pathology* / Q000601:surgery; D006023:Glycoproteins / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary* / Q000601:surgery; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000473:pathology*; D010455:Peptides / Q000276:immunology; D013525:Surgical Instruments; D015996:Survival Rate; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; C000606024:Prom1 protein, mouse