[Effects of calponin-1 gene silencing on the biological behavior of uterine smooth muscle cells].
OBJECTIVE
To study the effects of calponin-1 expression inhibition on the proliferation , invasiveness, apoptosis and cytoskeleton of uterine smooth muscle cells, and explore the molecular mechanism of calponin-1 in the uterine smooth muscle cells for labor onset.


METHODS
siRNA-calponin-1 adenovirus plasmid was constructed and transfected into primarily cultured uterine smooth muscle cells. The proliferation, invasiveness and apoptosis of the cells were determined by MTT assay, matrigel invasion assays and flow cytometry, respectively. Rhodamine-Phalloidin was used for labeling filamentous actin (F-actin), and the morphology and the distribution of F-actin was observed under fluorescence microscopy and analyzed quantitatively.


RESULTS
The motor ability of uterine smooth muscle cells decreased significantly after transfection with siRNA-calponin-1 adenovirus plasmid (P<0.05). The transfected cells showed thinner, loosened and irregular F-actin microfibers, and the cells in the empty vector and blank control groups showed thicker and longer F-actin microfibers.


CONCLUSIONS
Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro, suggesting that the morphological change and rearrangement of F-actin of uterine smooth muscle cell may be one of the important mechanisms in the labor onset.
Gu
Yong-hong
YH
Department of Pathology, Third Xiangya Hospital, Central South University, Changsha 410013, China. guyonghong 0815@yahoo.cn
Zhou
Chang-ju
CJ
Hu
Lin-yu
LY
Chen
Qian
Q
Zhang
Wei-she
WS
D017209:Apoptosis; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014599:Uterus / Q000166:cytology* / Q000378:metabolism; D000096985:Calponins
D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D000096985:Calponins