Cellulase deactivation based kinetic modeling of enzymatic hydrolysis of steam-exploded wheat straw.
Applying mass action law and quasi-steady-state theory, two cellulase kinetic models namely Eqs. (5) and (8) were developed on the basis of the first and second order reactions of enzyme deactivation, respectively. The two models are compared according to analysis of experimental data from enzymatic hydrolysis steam-exploded wheat straw. Both simulation and prediction results show Eq. (8) has much higher accuracy than Eq. (5). Analysis of initial hydrolysis rate is also in accordance with Eq. (8) and against Eq. (5). Fitted values of k(2) (the rate constant of product formation), k(de2) (the rate constant of enzyme deactivation) and K(e) (the equilibrium constant) determined from Eq. (8) are 0.4732 h(-1), 0.4011 L/(hg), and 16.8597 g/L, respectively. The higher the enzyme concentration is, the larger the deactivation rate.
Zhang
Yu
Y
Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Xu
Jing-Liang
JL
Xu
Hui-Juan
HJ
Yuan
Zhen-Hong
ZH
Guo
Ying
Y
D002480:Cellulase / Q000378:metabolism*; D003198:Computer Simulation; D004789:Enzyme Activation; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis; D007700:Kinetics; D008954:Models, Biological; D012044:Regression Analysis; D013227:Steam; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*
D013227:Steam; D002480:Cellulase; D005947:Glucose