Detection of s-triazine pesticides in natural waters by modified large-volume direct injection HPLC.
There is a need for simple and inexpensive methods to quantify potentially harmful persistent pesticides often found in our water-ways and water distribution systems. This paper presents a simple, relatively inexpensive method for the detection of a group of commonly used pesticides (atrazine, simazine and hexazinone) in natural waters using large-volume direct injection high performance liquid chromatography (HPLC) utilizing a monolithic column and a single wavelength ultraviolet-visible light (UV-vis) detector. The best results for this system were obtained with a mobile phase made up of acetonitrile and water in a 30:70 ratio, a flow rate of 2.0 mL min(-1), and a detector wavelength of 230 nm. Using this method, we achieved retention times of less than three minutes, and detection limits of 5.7 microg L(-1) for atrazine, 4.7 microg L(-1) for simazine and 4.0 microg L(-1) for hexazinone. The performance of this method was validated with an inter-laboratory trial against a National Association of Testing Authorities (NATA) accredited liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method commonly used in commercial laboratories.
Beale
David J
DJ
School of Applied Sciences, RMIT University, Melbourne, Australia.
Kaserzon
Sarit L
SL
Porter
Nichola A
NA
Roddick
Felicity A
FA
Carpenter
Peter D
PD
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057230:Limit of Detection; D010575:Pesticides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D014227:Triazines / Q000032:analysis*; D014867:Water; D014874:Water Pollutants, Chemical / Q000032:analysis*
D010575:Pesticides; D014227:Triazines; D014874:Water Pollutants, Chemical; D014867:Water