title: [Studies on chemistry component and the biological activity of petroleum ether extraction from pre-and post-processed of Cornus officinalis].
abstract: OBJECTIVE
  To investigate substance basis for improving pharmacodynamics by comparing the chemical constituents of petroleum ether extraction from crude and processed Cornus officinalis and the effects on the immunologic function of mice with immunosuppression induced.
  
  
  METHODS
  The volatile components in petroleum ether extraction were analyses by GC-MS. Non-specific immune function was determined by cleaning carbon particle index K, Swallow index a, spleen index and thymus index. The specific humoral immune function was evaluated by detecting the content of serum hemolysin.
  
  
  RESULTS
  The chemical constituents of petroleum ether extraction of Cornus officinalis before and after being processed had significant changes. After being processed, Vitamin E increased by 46.6%, linoleic acid increased by 18.3% and methyl linen increased by 30.9%. The extraction increased clearance rate of charcoal carbon particles index K, swallow index alpha, spleen index, thymus index and the level of serum hemolysin.
  
  
  CONCLUSIONS
  The extraction can markedly improve non-specific immune function and the specific humoral immune function which are active sites of improving immunologic function and post-processed is better. Substance basis could be Vitamin E, Linoleic acid, methyl linen and so on.
authors.affiliation: Department of Chemistry, Nanjing Agricutural University, Nanjing 210095, China.
authors.forename: Guan-ye
authors.initials: GY
authors.lastname: Li
authors.forename: Yun-xiang
authors.initials: YX
authors.lastname: Yao
authors.forename: Xia
authors.initials: X
authors.lastname: Ding
mesh_terms: D000818:Animals; D029768:Cornus / Q000737:chemistry*; D003520:Cyclophosphamide / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006460:Hemolysin Proteins / Q000097:blood; D007109:Immunity / Q000187:drug effects*; D007165:Immunosuppression Therapy; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects; D014810:Vitamin E / Q000032:analysis
chemical_list: D006460:Hemolysin Proteins; D010936:Plant Extracts; D014810:Vitamin E; D003520:Cyclophosphamide; D019787:Linoleic Acid