title: Lectin-like oxidized low-density lipoprotein receptor-1 is required for the adipose tissue expression of proinflammatory cytokines in high-fat diet-induced obese mice.
abstract: Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1) is a receptor for oxidized LDL, and is strongly expressed in endothelial cells at an early stage of atherosclerosis. LOX-1 expression in adipocytes is induced by PPARgamma (ligands and appears to be involved in adipocyte cholesterol metabolism. However, the role of adipose tissue LOX-1 in high-fat diet-induced obesity is unknown. We found that mRNA levels of adipose tissue LOX-1 were markedly increased in obese mice fed a high-fat diet (HFD) compared with those fed normal chow. The levels were closely correlated with those of a proinflammatory cytokine, monocyte chemoattractant protein-1 (MCP-1). Then, LOX-1 knockout (LOX-1-KO) and wild-type (WT) mice were fed HFD for 16weeks. HFD feeding increased the body and mesenteric fat weights similarly in WT and LOX-1-KO mice. HFD-induced expressions of proinflammatory cytokines such as MCP-1, MIP-1alpha, and IL-6 were significantly less in LOX-1-KO than WT mice. Thus, LOX-1 is required for the HFD-induced expression of proinflammatory cytokines in the adipose tissue of obese mice.
authors.affiliation: Division of Translational Research, National Hospital Organization Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimi-ku, Kyoto 612-8555, Japan.
authors.forename: Rieko
authors.initials: R
authors.lastname: Takanabe-Mori
authors.forename: Koh
authors.initials: K
authors.lastname: Ono
authors.forename: Naoya
authors.initials: N
authors.lastname: Sowa
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Wada
authors.forename: Tomohide
authors.initials: T
authors.lastname: Takaya
authors.forename: Takahiro
authors.initials: T
authors.lastname: Horie
authors.forename: Noriko
authors.initials: N
authors.lastname: Satoh-Asahara
authors.forename: Akira
authors.initials: A
authors.lastname: Shimatsu
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujita
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sawamura
authors.forename: Koji
authors.initials: K
authors.lastname: Hasegawa
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000502:physiology*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D016207:Cytokines; D004041:Dietary Fats; D051127:Scavenger Receptors, Class E