[Doxorubicin and menadione reduce cell proliferation of Saccharomyces cerevisiae by different mechanisms].
The aim of this study was to determine the impacts of doxorubicin and menadion on cell proliferation, cell distribution in accordance with the phases of the cell-cycle phase, glutathione concentration, ribonucleotide reductase expression, and Yap1 dependent redox-sensitive pathway activity using Saccharomyces cerevisiae as eukaryote cell model. Our data show that menadione induced cell cycle arrest in G1-phase, reduction of intracellular GSH, an increase in GSSG concentration, and dose-dependent increases in ribonucleotide reductase expression and the activity of Yap1 pathway. Doxorubicin induced cell cycle arrest in G1- and S-phases, increased GSH and GSSG concentrations, increased expression of ribonucleotide reductase, and modulated Yap-dependent pathway activity.
Saenko
Iu V
IuV
Shutov
A M
AM
Rastorgueva
E V
EV
D002453:Cell Cycle / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction; D012264:Ribonucleotide Reductases / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D024483:Vitamin K 3 / Q000494:pharmacology*
D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D024483:Vitamin K 3; D004317:Doxorubicin; D012264:Ribonucleotide Reductases; D005978:Glutathione