title: Chloride channel-dependent copper acquisition of laccase in the basidiomycetous fungus Cryptococcus neoformans.
abstract: The CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated pH. This study reports that copper homeostasis was impaired in the clc-a mutant. This was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption spectrometry. CLC-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in C. neoformans.
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: ChangWei
authors.initials: C
authors.lastname: Zhu
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Tianjin Industrial Microbiology Key Lab, College of Bioengineering, Tianjin University of Science & Technology, Tianjin, 300457, China.
authors.forename: DongGuang
authors.initials: D
authors.lastname: Xiao
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Pan
authors.affiliation: State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China. xudong82@nankai.edu.cn.
authors.forename: XuDong
authors.initials: X
authors.lastname: Zhu
mesh_terms: D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism* / Q000493:pharmacokinetics; D003455:Cryptococcus neoformans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic
chemical_list: D018118:Chloride Channels; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins; D003300:Copper; D042845:Laccase