title: Analysis of the effect of CPP-ACP tooth mousse on enamel remineralization by circularly polarized images.
abstract: OBJECTIVE
  To evaluate the effect of casein phosphopeptide-amorphous calcium phosphate tooth mousse on the remineralization of bovine incisor by circularly polarized images.
  
  
  METHODS
  Eighty bovine incisors, each with a 4 x 4 mm artificially demineralized area, were used. The samples were divided into four groups: Group A, casein phosphopeptide-amorphous calcium phosphate tooth mousse; Group B, fluoride toothpaste; Group C, casein phosphopeptide-amorphous calcium phosphate tooth mousse and fluoride toothpaste; and Group D, no treatment. Circularly polarized images were taken after the specimens were treated for 3, 6, 9, or 12 weeks, and the size of the demineralized area and the mean grey level were measured. Data analysis was done using repeated measures variance analysis. Pearson correlation coefficients were computed to evaluate the correlation between the size of the demineralized area and the mean grey level.
  
  
  RESULTS
  In all four groups, the size of the demineralized area and the mean grey level declined with time. The size of the demineralized area of Group C was significantly smaller than that of Group A at the end of the third and sixth weeks (P = .039, P = .000, respectively), and the mean grey level of Group C was lower than that of Group A at the end of the 6th and 12th weeks (P = .037, P = .004, respectively). At the end of the 6th, 9th, and 12th weeks, the size of the demineralized area of Group C was smaller (P = .000, P = .005, P = .005, respectively) and the mean grey level was lower (P = .000) than those of Group B. No statistically significant correlations were detected between the size of the demineralized area and the mean grey level.
  
  
  CONCLUSIONS
  Casein phosphopeptide-amorphous calcium phosphate tooth mousse can reduce the size and mean grey level of demineralized areas and promote the remineralization of bovine enamel. Combined application with fluoride toothpaste strengthens the effect.
authors.affiliation: Department of Orthodontics, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.
authors.forename: Guotao
authors.initials: G
authors.lastname: Wu
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Liu
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002364:Caseins / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects*; D004359:Drug Therapy, Combination; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007180:Incisor / Q000187:drug effects; D008422:Materials Testing; D008859:Microscopy, Polarization / Q000379:methods; D016910:Orthodontic Brackets; D011897:Random Allocation; D012464:Saliva, Artificial / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000628:therapy; D014089:Tooth Remineralization / Q000379:methods*; D014100:Toothpastes / Q000627:therapeutic use
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; D012464:Saliva, Artificial; D014100:Toothpastes; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides