title: Preparation and recognition performance of creatinine-imprinted material prepared with novel surface-imprinting technique.
abstract: By adopting the novel surface molecular imprinting technique put forward by us not long ago, a creatinine molecule-imprinted material with high performance was prepared. The functional macromolecule polymethacrylic acid (PMAA) was first grafted on the surfaces of micron-sized silica gel particles in the manner of "grafting from" using 3-methacryloxypropyltrimethoxysilane (MPS) as intermedia, resulting in the grafted particles PMAA/SiO(2). Subsequently, the molecular imprinting was carried out towards the grafted macromolecule PMAA using creatinine as template and with ethylene glycol diglycidyl ether (EGGE) as crosslinker by right of the intermolecular hydrogen bonding and electrostatic interaction between the grafted PMAA and creatinine molecules. Finally, the creatinine-imprinted material MIP-PMAA/SiO(2) was obtained. The binding character of MIP-PMAA/SiO(2) for creatinine was investigated in depth with both batch and column methods and using N-hydroxysuccinimide and creatine as two contrast substances, whose chemical structures are similar to creatinine to a certain degree. The experimental results show that the surface-imprinted material MIP-PMAA/SiO(2) has excellent binding affinity and high recognition selectivity for creatinine. Before imprinting, PMAA/SiO(2) particles nearly has not recognition selectivity for creatinine, and the selectivity coefficients of PMAA/SiO(2) for creatinine relative to N-hydroxysuccinimide and creatine are only 1.23 and 1.30, respectively. However, after imprinting, the selectivity coefficients of MIP-PMAA/SiO(2) for creatinine in respect to N-hydroxysuccinimide and creatine are remarkably enhanced to 11.64 and 12.87, respectively, displaying the excellent recognition selectivity and binding affinity towards creatinine molecules.
authors.affiliation: Department of Chemical engineering, North University of China, Taiyuan, Shanxi 030051, China. gaobaojiao@126.com
authors.forename: Baojiao
authors.initials: B
authors.lastname: Gao
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D003401:Creatine / Q000737:chemistry; D003404:Creatinine / Q000737:chemistry*; D004853:Epoxy Resins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D054802:Molecular Imprinting / Q000379:methods*; D011109:Polymethacrylic Acids / Q000737:chemistry; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D013696:Temperature
chemical_list: D004853:Epoxy Resins; D011109:Polymethacrylic Acids; D012997:Solvents; C030613:polymethacrylic acid; C035364:Quetol 651; D058428:Silica Gel; D012822:Silicon Dioxide; D003404:Creatinine; D003401:Creatine