[Calcium-binding Iba-1/AIF-1 protein in rat brain cells].
At present, calcium-binding Iba-1 protein is considered to be identical to AIF-1 protein and it is used as one of the selective microglia markers. However, the data on the cell populations capable of expressing Iba-1/AIF-1, are inconsistent. The aims of the present study were to identify rat brain cells expressing Iba-1 and to define their structural characteristics. The methods of immunocytochemistry and confocal laser microscopy were used. Iba-1 protein was detected only in microglial cells, macrophages of brain meninges, supraependymal macrophages, superficial and stromal cells of the choroid plexus--all the cells possessing phagocytotic function. Comparison of the data obtained with the results of other studies allows to suggest that not all of commonly used antibodies against Iba-1/AIF-1 demonstrate the same product. It can not be excluded, that it can be associated with the presence of alternative splicing of Iba-1 mRNA.
Kirik
O V
OV
Sukhorukova
E G
EG
Korzhevskiĭ
D E
DE
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008840:Microfilament Proteins; D018613:Microscopy, Confocal; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins