Dieckol isolated from Ecklonia cava inhibits alpha-glucosidase and alpha-amylase in vitro and alleviates postprandial hyperglycemia in streptozotocin-induced diabetic mice.
This study was designed to investigate whether dieckol may inhibit α-glucosidase and alpha-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. Dieckol isolated from Ecklonia cava, brown algae, evidenced prominent inhibitory effect against alpha-glucosidase and alpha-amylase. The IC(50) values of dieckol against alpha-glucosidase and alpha-amylase were 0.24 and 0.66 mM, respectively, which evidenced the higher activities than that of acarbose. Dieckol did not exert any cytotoxic effect in human umbilical vein endothelial cells (HUVECs) at various concentrations (from 0.33 to 2.69 mM). The increase of postprandial blood glucose levels were significantly suppressed in the dieckol administered group than those in the streptozotocin-induced diabetic or normal mice. Moreover, the area under curve (AUC) was significantly reduced via dieckol administration (259 versus 483 mmol min/l) in the diabetic mice as well as it delays absorption of dietary carbohydrates. Therefore, these result indicated that dieckol might be a potent inhibitor for α-glucosidase and α-amylase.
Lee
Seung-Hong
SH
Department of Marine life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Park
Mi-Hwa
MH
Heo
Soo-Jin
SJ
Kang
Sung-Myung
SM
Ko
Seok-Chun
SC
Han
Ji-Sook
JS
Jeon
You-Jin
YJ
D000818:Animals; D019540:Area Under Curve; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D042783:Endothelial Cells / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005087:Glucan 1,4-alpha-Glucosidase / Q000037:antagonists & inhibitors*; D006801:Humans; D006943:Hyperglycemia / Q000517:prevention & control*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D001572:Benzofurans; D001786:Blood Glucose; D004791:Enzyme Inhibitors; C503840:dieckol; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase