[Molecular basis of one-way serological reaction between SINV and XJ-160 virus].
The purpose of this study is to elucidate the molecular mechanism of one-way serological reaction between XJ-160 virus and SINV by recombinant viruses which exchanged the glycoprotein genes individually or simultaneously. Three recombinant viruses were obtained based on the whole-length infectious cDNA clone of XJ-160 virus. The infectivity and pathogenesis to BHK-21 cells and animals were studied and the gene which controlled this one-way serological reaction phenomenon was searched by MCPENT. The results showed that the E2 glycoprotein was the main factor which influenced the growth rate, plaque morphology and pathogenicity of BHK-21 cells and suckling mice. The results of MCPENT showed that the E2 glycoprotein of SINV played a major role in this one-way serological reaction phenomenon. Our study identified the SINE2 gene was the determined gene for one way serological reaction between XJ-160 virus and SINV, and this research laid the foundation for further analysis of the genomic structure and function of SINV.
Wang
Li-hua
LH
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC) and State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Beijing 100052, China. wanglih9755@hotmail.com.cn
Fu
Shi-hong
SH
Yang
Yi-liang
YL
Zhu
Wu-yang
WY
Tang
Qing
Q
Liang
Guo-dong
GD
D000524:Alphavirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics; D005260:Female; D005818:Genetic Engineering; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012845:Sindbis Virus / Q000276:immunology*; D019562:Viral Load; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
D004274:DNA, Recombinant; D006023:Glycoproteins; D014764:Viral Proteins