Identification of E. dysenterica laxative peptide: a novel strategy in the treatment of chronic constipation and irritable bowel syndrome.
Plants have contributed over the years to the discovery of various pharmacological products. Amongst the enormous diversity of herbs with remarkable medicinal use and further pharmacological potential, here in this report we evaluated pulp extracts from Eugenia dysenterica fruits and further identified the active principle involved in such laxative activity in rats. For protein isolation, fruits were macerated with an extraction solution following precipitation with (NH(4))(2)SO(4) (100%). After dialysis, the peptide was applied onto a reversed-phase semi-preparative HPLC column, and the major fraction was eluted with 26% and 66% acetonitrile. The evaluation of molecular masses by MALDI-TOF and Tris/Tricine SDS-PAGE of HPLC fractions showed the presence of a major peptide with approximately 7 kDa. The N-terminal amino acid peptide sequence was determined and showed no similarity to other proteins deposited in the Data Bank. Peptide from E. dysenterica was able to enhance rats' intestinal motility by approximately 20.8%, probably being responsible for laxative activity. Moreover, these proteins were non-toxic to mammals, as observed in histopathology and hemolytic analyses. In conclusion, results here reported indicate that, in the near future, proteins synthesized by E. dysenterica fruits could be utilized in the development of novel biotechnological pharmaceutics with laxative properties for use in chronic constipation and irritable bowel syndrome treatment.
Lima
T B
TB
Center for Proteomic and Biochemical Analyses, Post-Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasilia, SGAN Quadra 916, Modulo B, Av. W5, 70.790-160 Brasília, DF, Brazil.
Silva
O N
ON
Oliveira
J T A
JT
Vasconcelos
I M
IM
Scalabrin
F B
FB
Rocha
T L
TL
Grossi-de-Sá
M F
MF
Silva
L P
LP
Guadagnin
R V
RV
Quirino
B F
BF
Castro
C F S
CF
Leonardecz
E
E
Franco
O L
OL
D000595:Amino Acid Sequence; D000818:Animals; D001938:Brazil; D002908:Chronic Disease / Q000188:drug therapy; D003248:Constipation / Q000188:drug therapy*; D005638:Fruit / Q000009:adverse effects / Q000378:metabolism*; D005769:Gastrointestinal Motility / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology; D043183:Irritable Bowel Syndrome / Q000188:drug therapy*; D054368:Laxatives / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008519:Medicine, Traditional; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000009:adverse effects / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D027842:Syzygium / Q000378:metabolism*
D054368:Laxatives; D010455:Peptides; D010940:Plant Proteins