title: The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei.
abstract: BACKGROUND
  Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins (VAPs) and candidate receptor proteins involved in WSSV infection, allow a better understanding of how these proteins interact in the viral life cycle. In this study, our aim was to find some host cellular membrane proteins that could bind with white spot syndrome virus (WSSV).
  
  
  RESULTS
  Two proteins were evident by using a virus overlay protein binding assay (VOPBA) with WSSV. A protein with molecular weight 53 kDa, named BP53, was analyzed in this study, which was homologous with the F1-ATP synthase beta subunit by mass spectrometry analysis. Rapid amplification of cDNA ends (RACE) PCR was performed to identify the full-length cDNA of the bp53 gene. The resulting full-length gene consisted of 1836 bp, encoding 525 amino acids with a calculated molecular mass of 55.98 kDa. The deduced amino acid sequence contained three conserved domains of the F1-ATP synthase beta subunit. BP53 was therefore designated the F1-ATP synthase beta subunit of L. vannamei. The binding of WSSV to BP53 were also confirmed by competitive ELISA binding assay and co-immunoprecipitation on magnetic beads. To investigate the function of BP53 in WSSV infection, it was mixed with WSSV before the mixture was injected intramuscularly into shrimp. The resulting mortality curves showed that recombinant (r) BP53 could attenuate WSSV infection.
  
  
  CONCLUSIONS
  The results revealed that BP53 is involved in WSSV infection. Here is the first time showed the role of shrimp F1-ATP synthase beta subunit in WSSV infection.
authors.affiliation: Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, the Ministry of Agriculture; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Cheng
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D011485:Protein Binding; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D021122:Protein Subunits; D006180:Proton-Translocating ATPases