title: High density lipoprotein-anionic peptide factor effect on reverse cholesterol transport in type 2 diabetic patients with and without coronary artery disease.
abstract: OBJECTIVE
  To verify if HDL3 Anionic Peptide Factor (HDL3-APF) is as an apolipoprotein that promotes the reverse cholesterol transport.
  
  
  METHODS
  We investigated a possible association between plasma HDL3-APF concentration, cholesterol efflux from Fu5AH cells and cholesteryl ester transfer protein (CETP) activity in type 2 diabetic patients with coronary artery disease (CAD) (n=36), those without CAD (n=20), and 37 healthy subjects.
  
  
  RESULTS
  Plasma APF concentrations were decreased in diabetics with CAD compared to controls (p<0.01). Cellular cholesterol efflux was decreased in diabetics without and with CAD, (p<0.01 and p<0.001 respectively). CETP activity was significantly elevated in all patient groups. Multiple linear regression analysis shows that cholesterol efflux was independently and positively related only to APF concentrations in controls.
  
  
  CONCLUSIONS
  APF is likely to be a key independent factor for promoting cellular cholesterol efflux in healthy subjects. However this association is altered in type 2 diabetes.
authors.affiliation: Laboratoire de Biochimie, UR 'Nutrition et Désordres Métaboliques' Faculté de médecine, Monastir, Tunisia. Nabil.Attia@fsb.rnu.tn
authors.forename: Nebil
authors.initials: N
authors.lastname: Attia
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lorec
authors.forename: Nicole
authors.initials: N
authors.lastname: Domingo
authors.forename: Amel
authors.initials: A
authors.lastname: Nakbi
authors.forename: Sonia
authors.initials: S
authors.lastname: Hammami
authors.forename: Khaldoun
authors.initials: K
authors.lastname: Ben Hamda
authors.forename: Raja
authors.initials: R
authors.lastname: Châaba
authors.forename: Ahmed-Sleh
authors.initials: AS
authors.lastname: Masmoudi
authors.forename: Philippe
authors.initials: P
authors.lastname: Moulin
authors.forename: Henri
authors.initials: H
authors.lastname: Portugal
authors.forename: Denis
authors.initials: D
authors.lastname: Lairon
authors.forename: Françoise
authors.initials: F
authors.lastname: Chanussot
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami
mesh_terms: D000328:Adult; D001692:Biological Transport; D016022:Case-Control Studies; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism; D003324:Coronary Artery Disease / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000378:metabolism; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D002784:Cholesterol