Mast cell repopulation of the peritoneal cavity: contribution of mast cell progenitors versus bone marrow derived committed mast cell precursors.
BACKGROUND
Mast cells have recently gained new importance as immunoregulatory cells that are involved in numerous pathological processes. One result of these processes is an increase in mast cell numbers at peripheral sites. This study was undertaken to determine the mast cell response in the peritoneal cavity and bone marrow during repopulation of the peritoneal cavity in rats.


RESULTS
Two mast cell specific antibodies, mAb AA4 and mAb BGD6, were used to distinguish the committed mast cell precursor from more mature mast cells. The peritoneal cavity was depleted of mast cells using distilled water. Twelve hours after distilled water injection, very immature mast cells could be isolated from the blood and by 48 hours were present in the peritoneal cavity. At this same time the percentage of mast cells in mitosis increased fourfold. Mast cell depletion of the peritoneal cavity also reduced the total number of mast cells in the bone marrow, but increased the number of mast cell committed precursors.


CONCLUSIONS
In response to mast cell depletion of the peritoneal cavity, a mast cell progenitor is released into the circulation and participates in repopulation of the peritoneal cavity, while the committed mast cell precursor is retained in the bone marrow.
Jamur
Maria Célia
MC
Department of Cell and Molecular Biology and Pathogenic Bioagents, University of São Paulo, Ribeirão Preto, Brazil.
Moreno
Andréa N
AN
Mello
Luciana Fc
LF
Souza Júnior
Devandir A
DA
Campos
Maria Rita C
MR
Pastor
Maria Verônica D
MV
Grodzki
Ana Cristina G
AC
Silva
Deise C
DC
Oliver
Constance
C
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002452:Cell Count; D019070:Cell Lineage / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D005260:Female; D018189:Immunomagnetic Separation; D007274:Injections, Intraperitoneal; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D014867:Water / Q000008:administration & dosage / Q000494:pharmacology
D014867:Water