title: Breast tumor developed in a pregnant rat after treatment with the teratogen Cycloheximide.
abstract: OBJECTIVE
  To describe histochemically and immunohistochemically a breast tumor presented in a pregnant rat given Cycloheximide to examine its teratogenic and embryotoxic effect on the embryos.
  
  
  METHODS
  Cycloheximide was injected in pregnant Wistar rats at a dose of 3 mg/kg.b.w. on both 10th and 11th gestational days. In one of the rats, a large breast tumor developed rapidly. Histochemical staining with Hematoxylin-Eosin and Masson Trichrome and immunohistochemical identification with mouse monoclonal antibodies: a) Estrogen Receptor A and b) Estrogen Receptor B was performed.
  
  
  RESULTS
  Analysis with A-receptor and B-receptor showed that the breast tumor which was developed after treatment with Cycloheximide was malignant. Positive immunohistochemical reaction was evident especially with A-receptor indicating the malignancy of the tumor.
  
  
  CONCLUSIONS
  Cycloheximide is a known toxic and teratogenic agent and potentially a carcinogenic drug. Thus it should be used with extreme caution as a pesticide.
authors.forename: E N
authors.initials: EN
authors.lastname: Emmanouil-Nikoloussi
authors.forename: E
authors.initials: E
authors.lastname: Nikoloussis
authors.forename: M E
authors.initials: ME
authors.lastname: Manthou
authors.forename: O Ch
authors.initials: OCh
authors.lastname: Goula
authors.forename: Ch
authors.initials: Ch
authors.lastname: Likartsis
authors.forename: Th
authors.initials: T
authors.lastname: Papamitsou
authors.forename: H
authors.initials: H
authors.lastname: Frangou
authors.forename: S
authors.initials: S
authors.lastname: Massouridou
authors.forename: Ch
authors.initials: Ch
authors.lastname: Lazaridis
authors.forename: A
authors.initials: A
authors.lastname: Manthos