Transient expression of a vacuolar peroxidase increases susceptibility of epidermal barley cells to powdery mildew.
summary The expression of genes encoding the peroxidases, Prx7 and Prx8, is induced in barley leaf tissue after inoculation with the barley powdery mildew fungus, Blumeria graminis f.sp. hordei (DC) Speer (Bgh). The role of these peroxidases in general barley defence responses against fungal attack was investigated using a transient expression system. Colonization frequencies of Bgh on cells transfected with Prx7 or Prx8 expression-, mutant- or fusion-DNA constructs were compared to the frequencies on cells expressing a beta-glucuronidase (GUS) control construct. Twice the number of powdery mildew colonies were observed on cells expressing Prx7 as compared to control cells. Introduction of either mutant or truncated versions of Prx7 showed that decreased resistance against Bgh was dependent on the presence of the C-terminal signal peptide required for correct subcellular targeting, but not affected significantly by mutations in the catalytic centre. No impact on Bgh performance was observed after the introduction of Prx8 or mutant constructs. An enhanced accumulation of the apoplastic Prx8 was verified by immunocytology. These results indicate a more complex role of peroxidases in defence responses than was previously suspected.
Kristensen
B K
BK
Plant Research Department, Risø National Laboratory, PO Box 49, DK-4000 Roskilde, Denmark.
Ammitzbøll
H
H
Rasmussen
S K
SK
Nielsen
K A
KA