ChIP-Seq: a method for global identification of regulatory elements in the genome.
This unit describes ChIP-Seq methodology, which involves chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (Seq), and enables the genome-wide identification of binding sites of transcription factors (TFs) and other DNA-binding proteins. The process is initiated by cross-linking DNA and DNA-bound proteins. Subsequently, chromatin is isolated from nuclei and subjected to sonication. An antibody against a specific TF or DNA-binding protein is then used to immunoprecipitate specific DNA-TF complexes. ChIP DNA is purified, sequencing adapters are ligated, and 30- to 35-nucleotide (nt) sequence reads are generated. The sequence of the DNA fragments is mapped back to the reference genome for determination of the binding sites.
Raha
Debasish
D
Stanford University, Stanford, California, USA.
Hong
Miyoung
M
Snyder
Michael
M
D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D016678:Genome; D006801:Humans; D050436:Regulatory Elements, Transcriptional; D017422:Sequence Analysis, DNA