Staging of alpha-synuclein in the olfactory bulb in a model of Parkinson's disease: cell types involved.
Impaired olfaction is an early symptom of Parkinson's disease. The underlying neuropathology likely includes alpha-synucleinopathy in the olfactory bulb at an earlier stage (Braak's stage1) than pathology in the substantia nigra, which is not observed until stage 3. In this report, we investigated the distribution and cell types affected by alpha-synuclein in the olfactory bulb of transgenic mice (2-8 months of age) expressing the human A53T variant of alpha-synuclein. alpha-Synuclein immunostaining progressively affects interneurons and mitral cells. Double labeling studies demonstrate that dopaminergic cells are hardly involved, whereas glutamatergic- and calcium binding protein-positive cells are severely affected. This temporal evolution and the cell types expressing alpha-synuclein are reminiscent of idiopathic Parkinson's disease and support the usefulness of this model to address specific topics in the premotor phase of the disease.
Ubeda-Bañon
Isabel
I
Laboratorio de Neuroanatomía Humana, Departamento de Ciencias Médicas, Facultad de Medicina, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Spain.
Saiz-Sanchez
Daniel
D
de la Rosa-Prieto
Carlos
C
Mohedano-Moriano
Alicia
A
Fradejas
Noelia
N
Calvo
Soledad
S
Argandoña-Palacios
Lucia
L
Garcia-Muñozguren
Susana
S
Martinez-Marcos
Alino
A
D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism* / Q000473:pathology*; D010300:Parkinson Disease / Q000235:genetics / Q000473:pathology*; D010320:Parvalbumins / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
D010320:Parvalbumins; D014452:Ubiquitins; D051844:alpha-Synuclein; D014446:Tyrosine 3-Monooxygenase