Biosynthesis of medium-chain-length poly(3-hydroxyalkanoates) by volatile aromatic hydrocarbons-degrading Pseudomonas fulva TY16.
Pseudomonas fulva TY16 biosynthesized medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) containing unsaturated 3-hydroxydodecenoate unit (approximately 8-9%) when grown with volatile aromatic compounds including benzene, toluene, and ethylbenzene as sole carbon substrate. In particular, when cultivated using a continuous feeding system designed to supply toluene at a flow rate of 0.42gL(-1)h(-1) into a 7-L jar fermentor, the growth of the organism reached up to approximately 3.87gL(-1) after the 48h fed-batch fermentation, representing an accumulated cellular MCL-PHA of 58.9% by weight. The obtained MCL-PHA was a copolyester primarily consisting of 3-hydroxydecanoate (55.2%) and 3-hydroxyoctanoate (26.8%) with minor constituents being 3-hydroxyhexanoate (3.7%), 3-hydroxydodecenoate (8.2%), and 3-hydroxydodecanoate (6.1%). The present results suggest that P. fulva TY16 is a promising candidate for the biotechnological conversion of toxic petrochemical wastes to valuable biopolymers.
Ni
Yu-Yang
YY
Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Kim
Do Young
DY
Chung
Moon Gyu
MG
Lee
Sun Hee
SH
Park
Ho-Yong
HY
Rhee
Young Ha
YH
D001673:Biodegradation, Environmental / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology; D002210:Caprylates / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*; D014835:Volatilization / Q000187:drug effects
D002210:Caprylates; D006841:Hydrocarbons, Aromatic; D002244:Carbon