title: Secreted monocytic miR-150 enhances targeted endothelial cell migration.
abstract: MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate target gene expression at the posttranscriptional level. Here, we report that secreted miRNAs can serve as signaling molecules mediating intercellular communication. In human blood cells and cultured THP-1 cells, miR-150 was selectively packaged into microvesicles (MVs) and actively secreted. THP-1-derived MVs can enter and deliver miR-150 into human HMEC-1 cells, and elevated exogenous miR-150 effectively reduced c-Myb expression and enhanced cell migration in HMEC-1 cells. In vivo studies confirmed that intravenous injection of THP-1 MVs significantly increased the level of miR-150 in mouse blood vessels. MVs isolated from the plasma of patients with atherosclerosis contained higher levels of miR-150, and they more effectively promoted HMEC-1 cell migration than MVs from healthy donors. These results demonstrate that cells can secrete miRNAs and deliver them into recipient cells where the exogenous miRNAs can regulate target gene expression and recipient cell function.
authors.affiliation: Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Danqing
authors.initials: D
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Bian
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Jiuwei
authors.initials: J
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yin
authors.forename: Xing
authors.initials: X
authors.lastname: Cai
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Kehui
authors.initials: K
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ba
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Dongjin
authors.initials: D
authors.lastname: Wang
authors.forename: Junwei
authors.initials: J
authors.lastname: Yang
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yan
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000473:pathology; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb / Q000378:metabolism; D022142:Secretory Vesicles / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D035683:MicroRNAs; D020598:Proto-Oncogene Proteins c-myb; C533097:MIR150, human