title: Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison.
abstract: Complex exposure to xenobiotics is one of the reasons for the reported increase of respiratory diseases, cancer and immunological disturbances. Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3',4,4'-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5μM). The compounds PCB (250 microM) and AA (500 microM) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100 microM) and AA (500 microM) by one of the laboratories. Data generated in this study may assist in the interpretation of the mother/newborn biomonitoring study being carried out within project NewGeneris and will contribute to overall knowledge on the genotoxic potential of dietary/environmental toxicants.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia. jkatic@imi.hr
authors.forename: Jelena
authors.initials: J
authors.lastname: Katic
authors.forename: Eduardo
authors.initials: E
authors.lastname: Cemeli
authors.forename: Adolf
authors.initials: A
authors.lastname: Baumgartner
authors.forename: Julian
authors.initials: J
authors.lastname: Laubenthal
authors.forename: Irene
authors.initials: I
authors.lastname: Bassano
authors.forename: Solvor B
authors.initials: SB
authors.lastname: Stølevik
authors.forename: Berit
authors.initials: B
authors.lastname: Granum
authors.forename: Ellen
authors.initials: E
authors.lastname: Namork
authors.forename: Unni C
authors.initials: UC
authors.lastname: Nygaard
authors.forename: Martinus
authors.initials: M
authors.lastname: Løvik
authors.forename: Danitsja
authors.initials: D
authors.lastname: van Leeuwen
authors.forename: Kim
authors.initials: K
authors.lastname: Vande Loock
authors.forename: Diana
authors.initials: D
authors.lastname: Anderson
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Fucić
authors.forename: Ilse
authors.initials: I
authors.lastname: Decordier
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D048749:Cytokinesis / Q000187:drug effects; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005504:Food Analysis; D006801:Humans; D007753:Laboratories; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D011786:Quality Control; D015262:Xenobiotics / Q000633:toxicity*; D055815:Young Adult
chemical_list: D004785:Environmental Pollutants; D009153:Mutagens; D015262:Xenobiotics