title: Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging.
abstract: Ex vivo generated dendritic cells are currently used to induce therapeutic immunity in solid tumors. Effective immune response requires dendritic cells to home and remain in lymphoid organs to allow for adequate interaction with T lymphocytes. The aim of the current study was to detect and track Feridex labeled human dendritic cells in murine models using magnetic resonance imaging. Human dendritic cells were incubated with Feridex and the effect of labeling on dendritic cells immune function was evaluated. Ex vivo dendritic cell phantoms were used to estimate sensitivity of the magnetic resonance methods and in vivo homing was evaluated after intravenous or subcutaneous injection. R2*-maps of liver, spleen, and draining lymph nodes were obtained and inductively coupled plasma mass spectrometry or relaxometry methods were used to quantify the Feridex tissue concentrations. Correlations between in vivo R2* values and iron content were then determined. Feridex labeling did not affect dendritic cell maturation or function. Phantom results indicated that it was possible to detect 125 dendritic cells within a given slice. Strong correlation between in vivo R2* values and iron deposition was observed. Importantly, Feridex-labeled dendritic cells were detected in the spleen for up to 2 weeks postintravenous injection. This study suggests that magnetic resonance imaging may be used to longitudinally track Feridex-labeled human dendritic cells for up to 2 weeks after injection.
authors.affiliation: Department of Radiology, Mount Sinai School of Medicine, New York, New York 10029-6574, USA. karen.saebo@mountsinai.org
authors.forename: Karen C
authors.initials: KC
authors.lastname: Briley-Saebo
authors.forename: Marylene
authors.initials: M
authors.lastname: Leboeuf
authors.forename: Stephen
authors.initials: S
authors.lastname: Dickson
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Mani
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.forename: A Karolina
authors.initials: AK
authors.lastname: Palucka
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
mesh_terms: D000818:Animals; D058948:Cell Tracking; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000637:transplantation*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout