Allelic exchange in Actinomyces oris with mCherry fluorescence counterselection.
Described here is a method for facile generation of markerless gene deletion mutants of Actinomyces oris. Homologous integration of a nonreplicative vector carrying a gene exchange cassette into the bacterial chromosome was selected for by using mCherry fluorescence and resistance to kanamycin. Completion of allelic replacement was counterselected for by using loss of fluorescence.
Wu
Chenggang
C
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, USA.
Ton-That
Hung
H
D000190:Actinomyces / Q000235:genetics*; D000483:Alleles; D005453:Fluorescence; D017353:Gene Deletion; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional / Q000379:methods*; D011995:Recombination, Genetic; D012641:Selection, Genetic; D013194:Staining and Labeling / Q000379:methods