title: Regulation of suppressing and activating effects of mesenchymal stem cells on the encephalitogenic potential of MBP68-86-specific lymphocytes.
abstract: Mesenchymal stem cells (MSCs) possess immunosuppressive potential and are proposed as a tool for cell therapy, however under certain circumstance they could change to become immunostimulating. It is therefore of great importance to better understand the MSCs' immune plasticity. Here we used different doses of MSCs to co-culture with MBP(68-86)-specific T cells and found that MSCs exerted a suppressive effect on the encephalitogenic ability of MBP(68-86)-specific T cells at high MSC density (MSC/effector ratio ≥ 1:10). Whereas at lower MSC/effector ratios (≤ 1:50), MSCs shifted towards stimulatory activity. Thus the limited amounts of MSCs for application should be considered.
authors.affiliation: Department of Neurobiology, Harbin Medical University, Heilongjiang Provincial Key Laboratory of Neurobiology, Harbin, Heilongjiang, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Guangyou
authors.initials: G
authors.lastname: Wang
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Kong
authors.forename: Lili
authors.initials: L
authors.lastname: Mu
authors.forename: Jinghua
authors.initials: J
authors.lastname: Wang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Yi
authors.initials: Y
authors.lastname: An
authors.forename: Hulun
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D004676:Myelin Basic Protein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D004676:Myelin Basic Protein; D010446:Peptide Fragments