Microbial production of acetoacetate by recombinant Escherichia coli.
A synthetic pathway consisting of octAB encoding succinyl-CoA:acetoacetate CoA-transferase and phaA encoding beta-ketothiolase from Ralstonia eutropha H16 was constructed in Escherichia coli for extracellular production of acetoacetate. The recombinant E. coli produced 8.2g/l acetoacetate in shake flasks at 37 degrees C in 24h. In a 6-L fermentor, extracellular acetoacetate production was enhanced to 13.1g/l after 30h of fed-batch growth. To our knowledge, this is the first study on microbial production of acetoacetate.
Hu
Die
D
Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Life Science, Tsinghua University, Beijing 100084, China.
Shi
Zhenyu
Z
Wu
Qiong
Q
Chen
Guo-Qiang
GQ
D000090:Acetoacetates / Q000378:metabolism*; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics*; D005110:Extracellular Space / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways; D043367:Ralstonia / Q000201:enzymology / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics
D000090:Acetoacetates; C016635:acetoacetic acid