title: Comparison of neuraminidase activity of influenza A virus subtype H5N1 and H1N1 using reverse genetics virus.
abstract: Neuraminidase (NA) is an envelope surface glycoprotein of influenza A viruses. It cleaves alpha-(2,3) or alpha-(2,6) glycosidic linkage between a terminal sialic acid residue of the host cell receptor and hemagglutinin of the viral envelope, thus releasing viral progeny from the infected cell. In this study, a reassortant virus (H1N1-NA-H5N1) containing the NA gene from A/duck/Phitsanulok/ NIAH6-5-0001/2007 (H5N1) virus and seven remaining genetic segments from A/ Puerto Rico/8/1934 (H1N1) was constructed using reverse genetic technique. NA activity of H1N1-NA-H5N1 virus was lower than that of A/Puerto Rico/8/1934 (H1N1), and NA activity of A/duck/Phitsanulok/NIAH6-5-0001/2007 study (H5N1) was the lowest among them (p < 0.05). To our knowledge, this is the first comparative study of NA activity of H1N1 and H5N1 virus using reverse genetic technique. It also indicates that the NA gene may be expressed at a higher level in the H1N1 infected cell than the H5N1 infected cell.
authors.affiliation: Faculty of Medical Sciences, Naresuan University, Phitsanulok, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Rawangkhan
authors.forename: Donruedee
authors.initials: D
authors.lastname: Sanguansermsri
authors.forename: Narutchala
authors.initials: N
authors.lastname: Suwannakhon
authors.forename: Sutatip
authors.initials: S
authors.lastname: Pongcharoen
authors.forename: Purinthra
authors.initials: P
authors.lastname: Pensuwan
authors.forename: Chanpen
authors.initials: C
authors.lastname: Chamnanpood
authors.forename: Pornchai
authors.initials: P
authors.lastname: Chamnanpood
authors.forename: Phanchana
authors.initials: P
authors.lastname: Sanguansermsri
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000201:enzymology*; D053124:Influenza A Virus, H5N1 Subtype / Q000201:enzymology*; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D016865:Reassortant Viruses / Q000201:enzymology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D009439:Neuraminidase