title: Effects of COLIA1 polymorphisms and haplotypes on perimenopausal bone mass, postmenopausal bone loss and fracture risk.
abstract: One thousand seven hundred seventeen perimenopausal women from the Danish Osteoporosis Prevention Study were genotyped for the -1997G/T, -1663indelT and +1245G/T polymorphisms in the COLIA1 gen. We found that the -1997T allele and a haplotype containing it were associated with reduced bone mineral density (BMD) and increased bone turnover at menopause and after 10 years of follow-up.
  
  
  BACKGROUND
  We wanted to investigate whether the -1997G/T, -1663indelT and +1245G/T polymorphisms in the COLIA1 gene are associated with perimenopausal bone mass, early postmenopausal bone loss and interact with hormone treatment.
  
  
  METHODS
  One thousand seven hundred seventeen perimenopausal women from the Danish Osteoporosis Prevention Study were genotyped, and haplotypes were determined. BMD was examined by dual X-ray absorptiometry.
  
  
  RESULTS
  Women carrying the -1997T variant had lower BMD at all measured sites: lumbar spine BMD 1.030 ± 0.137 g/cm(2), 1.016 ± 0.147 g/cm(2) and 0.988 ± 0.124 g/cm(2) in women with the GG, GT and TT genotypes, respectively (p < 0.05) and total hip BMD 0.921 ± 0.116 g/cm(2), 0.904 ± 0.123 g/cm(2) and 0.887 ± 0.109 g/cm(2) in women with the GG, GT and TT genotypes, respectively (p = 0.01). The effect remained after 10 years although statistical significance was lost. Haplotype 3 (-1997T-1663ins + 1245G) was associated with lower bone mass and higher levels of bone turnover. Compared with haplotype 1, haplotype 3 carriers had lower BMD at the lumbar spine, femoral neck and total hip by 0.016 ± 0.007 g/cm(2), 0.015 ± 0.006 g/cm(2) and 0.017 ± 0.006 g/cm(2), respectively (p < 0.05-0.005). No association with postmenopausal changes in bone mass and fracture risk and no overall interaction with the effects of hormone therapy could be demonstrated for any of the polymorphisms in COLIA1.
  
  
  CONCLUSIONS
  The -1997G/T polymorphism and haplotype 3 are significantly associated with perimenopausal bone mass, and these effects were sustained up to 10 years after menopause. No association between the -1663indelT or +1245G/T polymorphisms and peri- or postmenopausal bone mass could be demonstrated.
authors.affiliation: Department of Endocrinology and Internal Medicine THG, Aarhus University Hospital, Aarhus, Denmark. nuria.gonzalez.bofill@ki.au.dk
authors.forename: N
authors.initials: N
authors.lastname: González-Bofill
authors.forename: L B
authors.initials: LB
authors.lastname: Husted
authors.forename: T
authors.initials: T
authors.lastname: Harsløf
authors.forename: C L
authors.initials: CL
authors.lastname: Tofteng
authors.forename: B
authors.initials: B
authors.lastname: Abrahamsen
authors.forename: P
authors.initials: P
authors.lastname: Eiken
authors.forename: P
authors.initials: P
authors.lastname: Vestergaard
authors.forename: B L
authors.initials: BL
authors.lastname: Langdahl
mesh_terms: D015519:Bone Density / Q000235:genetics*; D024042:Collagen Type I / Q000235:genetics*; D000090602:Collagen Type I, alpha 1 Chain; D015914:Estrogen Replacement Therapy; D005260:Female; D005272:Femur Neck / Q000503:physiopathology; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006621:Hip Joint / Q000503:physiopathology; D006801:Humans; D008159:Lumbar Vertebrae / Q000503:physiopathology; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000503:physiopathology / Q000517:prevention & control; D058866:Osteoporotic Fractures / Q000235:genetics* / Q000503:physiopathology / Q000517:prevention & control; D047648:Perimenopause / Q000502:physiology; D020641:Polymorphism, Single Nucleotide
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain