A novel capillary electrophoretic method for determining methylglyoxal and glyoxal in urine and water samples.
Two non-electroactive biomarkers methylglyoxal (MGo) and glyoxal (Go) in urine and environmental water samples were determined for the first time by capillary electrophoresis with amperometric detection (CE-AD) after derivatizing with an electroactive compound 2-thiobarbituric acid. Experimental conditions of derivatization and CE-AD detection were optimized. Highly linear response was obtained for these two biomarkers over three orders of magnitude with good correlation (r(2)>0.999). The limits of detection (LODs) and limits of quantitation (LOQs) of MGo and Go were 0.2microgL(-1) and 1.0microgL(-1), 0.5microgL(-1) and 2.0microgL(-1), respectively. The average recovery and relative standard deviation (RSD) were within the range of 90.9-101.3% and 0.7-2.2%, respectively. The proposed CE-AD method provides a reliable and sensitive quantitative evaluation of MGo and Go in real sample matrices by employing relatively simple and inexpensive instrument.
Zhang
Junbo
J
Department of Chemistry, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
Zhang
Haitao
H
Li
Mengjie
M
Zhang
Dongli
D
Chu
Qingcui
Q
Ye
Jiannong
J
D019075:Electrophoresis, Capillary / Q000379:methods*; D006037:Glyoxal / Q000032:analysis* / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D011765:Pyruvaldehyde / Q000032:analysis* / Q000652:urine
D006037:Glyoxal; D011765:Pyruvaldehyde