Label-free optical diagnosis of hepatitis B virus with genetically engineered fusion proteins.
A simple biosensing strategy for the diagnosis of patients with hepatitis B virus (HBV) was developed. This study can be divided into two themes, both of which utilized gold-binding polypeptide (GBP) fusion proteins: HBV surface antigen PreS2 (HBsAg) detection with GBP-fused single chain antibody (GBP-ScFv) and anti-HBsAg detection with GBP-HBsAg. These GBP-fusion proteins can directly bind onto the gold surface via the high binding affinity between the GBP and the gold surface, while at the same time, orient the recognition sites toward the sample for target binding. This one-step immobilization strategy, which greatly simplifies a fabrication process as well as maintaining biological activity of the recognition elements, can be applied to optical analytical methods, such as surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR).
Zheng
Shun
S
Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Yuseong-gu, Daejeon, Republic of Korea.
Kim
Do-Kyun
DK
Park
Tae Jung
TJ
Lee
Seok Jae
SJ
Lee
Sang Yup
SY
D055910:Antibodies, Immobilized / Q000276:immunology* / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D006509:Hepatitis B / Q000175:diagnosis* / Q000276:immunology; D006515:Hepatitis B virus; D006801:Humans; D055095:Optics and Photonics; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties
D055910:Antibodies, Immobilized; D011993:Recombinant Fusion Proteins; D006046:Gold