title: Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain.
abstract: Diffusion tensor imaging is gaining increasing importance for anatomical imaging of the developing mouse brain. However, the application of diffusion tensor imaging to mouse brain imaging at microscopic levels is hindered by the limitation on achievable spatial resolution. In this study, fast diffusion tensor microimaging of the mouse brain, based on a diffusion-weighted gradient and spin echo technique with twin-navigator echo phase correction, is presented. Compared to echo planar and spin echo acquisition, the diffusion-weighted gradient and spin echo acquisition resulted in significant reduction in scan time and had minimal image distortion, thereby allowing acquisition at higher spatial resolution. In this study, three-dimensional diffusion tensor microimaging of the mouse brains at spatial resolutions of 50-60 microm revealed unprecedented anatomical details. Thin fiber bundles in the adult striatum and white matter tracts in the embryonic day 12 mouse brains were visualized for the first time. The study demonstrated that data acquired using the diffusion tensor microimaging technique allow three-dimensional mapping of gene expression data and can serve as a platform to study gene expression patterns in the context of neuroanatomy in the developing mouse brain.
authors.affiliation: Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. maggarw2@jhu.edu
authors.forename: Manisha
authors.initials: M
authors.lastname: Aggarwal
authors.forename: Susumu
authors.initials: S
authors.lastname: Mori
authors.forename: Tomomi
authors.initials: T
authors.lastname: Shimogori
authors.forename: Seth
authors.initials: S
authors.lastname: Blackshaw
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000196:embryology / Q000254:growth & development; D002874:Chromosome Mapping; D056324:Diffusion Tensor Imaging / Q000379:methods*; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011859:Radiography
chemical_list: D018398:Homeodomain Proteins