[Forward genetic screening for zebrafish mutants defective in myelopoiesis].
OBJECTIVE
To identify zebrafish mutants with myelopoiesis defects by ENU mutagenesis and large-scale forward genetic screening.


METHODS
Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in the spermatogonial cells to generate the founders, which were outcrossed with AB to raise F1 fish. The F1 fish from different founders were mated to generate the F2 families. The F3 embryos from F2 sibling crosses were screened by Sudan black B staining and neutral red staining.


RESULTS
A total of 350 F2 families from F1 sibling crosses were screened, and 1424 F2 crosses were analyzed. Six mutations were identified resulting in abnormal Sudan black B staining and neutral red staining, indicating the involvement of neutrophil deficiency or macrophage abnormalities.


CONCLUSIONS
It is simple and cheap to induce and screen myelopoiesis deficiency in zebrafish by ENU chemical mutagenesis and Sudan black B staining and neutral red staining. These mutants shed light on the identification of the genes important to myelopoiesis in zebrafish.
Dai
Zhao-xia
ZX
Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. daizhaoxia@yahoo.cn
Yan
Guang
G
Chen
Ying-hua
YH
Liu
Wei
W
Huo
Zhong-jun
ZJ
Wen
Zong-hua
ZH
Liu
Jing
J
Wang
Kun
K
Huang
Zhi-bing
ZB
Ma
Ning
N
Chen
Xiao-hui
XH
Ma
Ping-yun
PY
Luo
Wei-hao
WH
Zhao
Ying
Y
Fan
Shu
S
Huang
Hong-hui
HH
Wen
Zi-long
ZL
Zhang
Wen-qing
WQ
D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005820:Genetic Testing; D008297:Male; D016296:Mutagenesis; D009154:Mutation; D023461:Myeloid Progenitor Cells / Q000502:physiology*; D038042:Myelopoiesis / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*