Rheology of intervertebral disc: an ex vivo study on the effect of loading history, loading magnitude, fatigue loading, and disc degeneration.
METHODS
An ex vivo biomechanical study on the rheological properties of healthy porcine and degenerated human intervertebral disc.


OBJECTIVE
To quantify the effect of loading history, loading magnitude, fatigue loading, and degeneration on disc rheology.


BACKGROUND
Disc rheological parameters, i.e., the aggregate modulus (HA) and hydraulic permeability (k) regulate the mechanical and biologic function of disc. The knowledge of effects of loading condition and degeneration on disc rheology can be beneficial for the design of new disc/nucleus implants or therapy.


METHODS
The following 4 phases of experiments were conducted to find the changes of disc rheological properties: (1) Effect of loading history during 1-hour creep (640 N) and relaxation (20% strain) test. (2) Effect of loading magnitude (420 N vs. 640 N) during the creep test. (3) Effect of fatigue loading (420 N, 5 Hz for 0.5, 1, and 2 hours) on the creep loading behavior. (4) Difference of healthy porcine and degenerated human discs during creep loading. The experimental data were fitted with linear biphasic model.


RESULTS
The aggregate modulus increased but hydraulic permeability decreased during creep loading. The aggregate modulus decreased but the hydraulic permeability did not change significantly during relaxation loading. The higher creep loading increased the aggregate modulus but decreased the hydraulic permeability. The fatigue loading did not change the aggregate modulus significantly, but decreased hydraulic permeability. Comparing the degenerated human disc to the healthy porcine disc, the aggregate modulus was higher but the hydraulic permeability was lower.


CONCLUSIONS
The external loading and degeneration induce disc structural changes, e.g., the disc water content and interstitial matrix porosity, hence affect the disc rheological properties. The increase of aggregate modulus may be due to the reduction of disc hydration level, whereas the decrease of hydraulic permeability may be because of the shrinkage of disc matrix pores.
Kuo
Ya-Wen
YW
College of Medicine and Engineering, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
Wang
Jaw-Lin
JL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology*; D002102:Cadaver; D045604:Extracellular Fluid / Q000502:physiology; D005260:Female; D051445:Fibrocartilage / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007403:Intervertebral Disc / Q000473:pathology / Q000503:physiopathology*; D007405:Intervertebral Disc Displacement / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D008953:Models, Anatomic; D018763:Muscle Fatigue / Q000502:physiology*; D012212:Rheology / Q000379:methods; D034421:Sus scrofa; D016474:Weight-Bearing / Q000502:physiology