title: Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells.
abstract: The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host.
authors.affiliation: Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH J2-506, Station 6, 1015 Lausanne, Switzerland.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Isabel Fernandez
authors.initials: IF
authors.lastname: Garcia
authors.forename: Lucia
authors.initials: L
authors.lastname: Baldi
authors.forename: David L
authors.initials: DL
authors.lastname: Hacker
authors.forename: Florian M
authors.initials: FM
authors.lastname: Wurm
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry; D009997:Osmotic Pressure; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012462:Saline Solution, Hypertonic / Q000378:metabolism
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D012462:Saline Solution, Hypertonic