Good cell, bad cell: flow cytometry reveals T-cell subsets important in HIV disease.
Flow cytometry is a key technology in the study of HIV disease. In this article, we review various cellular markers that can be measured in the setting of pathogenesis or vaccination studies, including markers of activation, differentiation, senescence, immune suppression, and function. In addition, we discuss important considerations for making these measurements. Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future.
Chattopadhyay
Pratip K
PK
Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. pchattop@mail.nih.gov
Roederer
Mario
M
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D016922:Cellular Senescence / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D015415:Biomarkers