Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan.
Bartonella henselae is the causative agent of cat scratch disease (CSD). To clarify the population structure and relationship between human and cat strains of B. henselae, 55 specimens isolated in Japan, including 24 B. henselae DNA-positive clinical samples from CSD patients and 31 B. henselae isolates from domestic cats, were characterized by multilocus sequence typing (MLST) and the 16S-23S tRNA-Ala/tRNA-Ile intergenic spacer (S1) sequence, which were used previously for strain typing of B. henselae. Three different sequence types (STs) were identified by MLST, one of which was novel. Fifty-two strains (94.5%), including all strains detected in CSD patients, were assigned to ST-1. Eight S1 genotypes were observed, three of which were novel. The 52 ST-1 strains were classified into seven S1 genotypes, two of which were predominant in both human and cat strains. In addition, 5.5% of the strains (3/55) contained two different intergenic spacer S1 copies. These results indicate that the predominant B. henselae MLST ST-1 in Japan is a significantly genetically diverse population on the basis of the sequence diversity of intergenic spacer S1, and that highly prevalent S1 genotypes among cats are often involved in human infections.
Yanagihara
Masashi
M
Department of Basic Laboratory Sciences, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan. m-yanagi@yamaguchi-u.ac.jp
Tsuneoka
Hidehiro
H
Hoshide
Shoko
S
Ishido
Erina
E
Umeda
Akiko
A
Tsukahara
Masato
M
Nojima
Junzo
J
Ichihara
Kiyoshi
K
Hino
Keisuke
K
Hirai
Itaru
I
Yamamoto
Yoshimasa
Y
D000818:Animals; D018416:Bartonella henselae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002372:Cat-Scratch Disease / Q000382:microbiology* / Q000662:veterinary*; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D058889:Molecular Typing
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer