[A heterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens].
A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta laccase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing. Activity of the secreted heterologous laccase in the culture liquid reaches 3 U/ml, accounting for 98% of the total laccase activity, which demonstrates a high efficiency ofheterologous secretion. The synthesized P. canescens laccase has the same molecular weight as the enzyme produced by T. hirsuta 072.
Abianova
A R
AR
Chulkin
A M
AM
Vavilova
E A
EA
Fedorova
T V
TV
Loginov
D S
DS
Koroleva
O V
OV
Benevolenskiĭ
S V
SV
D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D042845:Laccase; D010407:Penicillium / Q000235:genetics / Q000254:growth & development*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D055454:Trametes / Q000201:enzymology* / Q000235:genetics
D005656:Fungal Proteins; D011994:Recombinant Proteins; D042845:Laccase