title: Human metabolites of brevetoxin PbTx-2: Identification and confirmation of structure.
abstract: Four metabolites were identified upon incubation of brevetoxin (PbTx-2) with human liver microsomes. Chemical transformation of PbTx-2 confirmed the structures of three known metabolites BTX-B5, PbTx-9 and 41, 43-dihydro-BTX-B5 and a previously unknown metabolite, 41, 43-dihydro-PbTx-2. These metabolites were also observed upon incubation of PbTx-2 with nine human recombinant cytochrome P450s (1A1, 1A2, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5). Cytochrome P450 3A4 produced oxidized metabolites while other CYPs generated the reduced products.
authors.affiliation: Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA.
authors.forename: Fujiang
authors.initials: F
authors.lastname: Guo
authors.forename: Tianying
authors.initials: T
authors.lastname: An
authors.forename: Kathleen S
authors.initials: KS
authors.lastname: Rein
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008387:Marine Toxins / Q000737:chemistry / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D035964:Oxocins; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D008387:Marine Toxins; D035964:Oxocins; C051732:Ptychodiscus brevis T2 toxin; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System