Bisulfite sequencing of DNA.
Exact positions of 5-methylcytosine (m(5)C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed.
Darst
Russell P
RP
University of Florida College of Medicine, Gainesville, Florida, USA.
Pardo
Carolina E
CE
Ai
Lingbao
L
Brown
Kevin D
KD
Kladde
Michael P
MP
D044503:5-Methylcytosine / Q000032:analysis*; D004247:DNA / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites / Q000378:metabolism*
D013447:Sulfites; D044503:5-Methylcytosine; D004247:DNA; C042345:hydrogen sulfite