Direct and rapid electrochemical immunosensing system based on a conducting polymer.
A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen-antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.
Piro
B
B
Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), associé au CNRS-UMR 7086, Université Paris Diderot-Paris 7, Paris, France.
Zhang
Q D
QD
Reisberg
S
S
Noel
V
V
Dang
L A
LA
Duc
H T
HT
Pham
M C
MC
D019342:Acetic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D009285:Naphthoquinones / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D044967:Serum / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013997:Time Factors
D004800:Enzymes, Immobilized; D009285:Naphthoquinones; D011108:Polymers; D013438:Sulfhydryl Compounds; D019342:Acetic Acid; C035342:1,4-naphthoquinone