Analysis of gelatin plasma substitutes in blood based on detection of hydroxyproline derivatives.
The gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.
Liu
Tao
T
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
Zhanga
Guifeng
G
Li
Suping
S
Wang
Yinjue
Y
Ma
Guanghui
G
Su
Zhiguo
Z
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism; D001810:Blood Volume / Q000187:drug effects; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000097:blood; D005780:Gelatin / Q000009:adverse effects / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D006207:Half-Life; D006470:Hemorrhage / Q000097:blood; D006868:Hydrolysis; D006909:Hydroxyproline / Q000097:blood* / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000737:chemistry; D039823:Iridoids; D008297:Male; D010952:Plasma Substitutes / Q000009:adverse effects / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D011817:Rabbits; D013387:Succinic Anhydrides / Q000737:chemistry
D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D010952:Plasma Substitutes; D013387:Succinic Anhydrides; C031801:succinic anhydride; D005780:Gelatin; C007834:genipin; D004139:Dinitrofluorobenzene; D006909:Hydroxyproline