Conformational organizations of G-quadruplexes composed of d(G(4)T(n))(3)G(4).
Structural polymorphism is one of the important issues with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and their physical property in nano-material. A series of oligonucleotides with four repeat guanines sequence [d(G(4)T(n))(3)G(4) (n=1-6)] were designed. In this study, the effects of loop length on the formation of structures of G-quadruplex were investigated through the result of CD (circular dichroism) and 20% non-denatured polyacrylamide gel electrophoresis. Our studies demonstrate that the length of loop in 100mM KCl solution could predict the conformation of G-quadruplex.
Wong
Wan Chi
WC
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
Zhuang
Jinyi
J
Ng
Selina Ling Ling
SL
New
Lilian Li Lin
LL
Hiew
Shuhui
S
Guo
Juanjuan
J
Yang
Zhaoqi
Z
Li
Tianhu
T
D001483:Base Sequence; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry
D009841:Oligonucleotides; D006147:Guanine