title: A-type lamin dynamics in bovine somatic cell nuclear transfer embryos.
abstract: The persistence of A-type nuclear lamin in somatic cell nuclear transfer (SCNT) embryos has been proposed as a marker for incomplete nuclear reprogramming. Using monoclonal antibodies to A/C- (A/C-346 and A/C-131C3) and B-type lamin, we compared distribution during early development of bovine IVF, parthenogenetic and SCNT embryos. A/C-346 staining was observed in the pronuclei of IVF embryos and in nuclei at the two-cell stage, but was not detected in subsequent cleavage stages up to and including hatched blastocysts. In contrast, A/C-131C3 and anti-lamin B2 stained all preimplantation stage embryos. Parthenogenetic and SCNT embryos had similar staining patterns to IVF embryos for all three antibodies, demonstrating correct nuclear architecture reprogramming. Inhibiting protein synthesis with cycloheximide (CHX) in parthenogenetic and SCNT embryos did not affect lamin A/C localisation, suggesting that lamin A/C is maternal in origin. However, activation with CHX delayed lamin A/C incorporation compared with 6-dimethylaminopurine activation. In SCNT embryos, staining for both A/C- and B-type lamin was delayed compared with parthenotes, although lamin B2 incorporation preceded lamin A/C in both. In conclusion, the lamin A/C distribution in SCNT bovine embryos paralleled that of IVF and parthenogenetic controls and therefore is not a marker of incomplete reprogramming.
authors.affiliation: Animal Development and Biotechnology Group, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, UK.
authors.forename: Richard D W
authors.initials: RD
authors.lastname: Kelly
authors.forename: Ramiro
authors.initials: R
authors.lastname: Alberio
authors.forename: Keith H S
authors.initials: KH
authors.lastname: Campbell
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D005307:Fertilization in Vitro; D007150:Immunohistochemistry; D034904:Lamin Type A / Q000378:metabolism*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism*; D010312:Parthenogenesis
chemical_list: D034904:Lamin Type A