Analysis and prediction of VH/VL packing in antibodies.
The packing of V(H) and V(L) domains in antibodies can vary, influencing the topography of the antigen-combining site. However, until recently, this has largely been ignored in modelling antibody structure. We present an analysis of the degree of variability observed in known structures together with a machine-learning approach to predict the packing angle. A neural network was trained on sets of interface residues and a genetic algorithm designed to perform 'feature selection' to define which sets of interface residues could be used most successfully to perform the prediction. While this training procedure was very computationally intensive, prediction is performed in a matter of seconds. Thus, not only do we provide a rapid method for predicting the packing angle, but also we define a set of residues that may be important in antibody humanization in order to obtain the correct binding site topography.
Abhinandan
K R
KR
Institute of Structural and Molecular Biology, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK.
Martin
Andrew C R
AC
D000465:Algorithms; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000737:chemistry* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D016571:Neural Networks, Computer; D011487:Protein Conformation; D012044:Regression Analysis
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region