title: A functional promoter polymorphism -607G>C of WNT10B is associated with abdominal fat in Korean female subjects.
abstract: WNT10B has been implicated as a potential regulator of adipogenesis in cellular and animal models of obesity. In this study, we attempted to characterize the associations between common genetic polymorphisms of WNT10B and fat accumulation in a sample of 1029 Korean female subjects. Direct sequencing of genomic DNAs of 45 subjects identified six common single-nucleotide polymorphisms (SNPs) of WNT10B, which were in almost complete linkage disequilibrium. Among the six SNPs, -607G>C (rs833840) showed differential nuclear factor binding in an electrophoretic mobility shift assay and differential promoter activity in a reporter assay, implicating it as a functional regulatory SNP. When body compositions of the subjects determined using bio-impedance analysis were compared according to their -607G>C genotype, only body fat mass showed a significant association. Body masses of protein, mineral and water showed no association. For more accurate evaluation of the effects of -607G>C genotype on body fat, cross-sectional fat areas of the subjects measured by abdominal computed tomography were compared. Genotype of -607G>C was significantly associated with abdominal total fat and abdominal subcutaneous fat areas (P=.009 and P=.007 in recessive model, respectively). Of the 1029 subjects, 576 were treated with a 1 month very low calorie diet and changes of body weight and composition were compared with -607G>C genotype. No significant associations were evident. This study is the first report of the association of common genetic polymorphism of WNT10B with human fat accumulation.
authors.affiliation: Department of Biology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Il Chul
authors.initials: IC
authors.lastname: Kim
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Cha
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Haeyong
authors.initials: H
authors.lastname: Lee
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Moon
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Choi
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Yoosik
authors.initials: Y
authors.lastname: Yoon
mesh_terms: D050153:Abdominal Fat / Q000378:metabolism*; D000328:Adult; D000704:Analysis of Variance; D044466:Asian People / Q000235:genetics; D001823:Body Composition; D001835:Body Weight; D031204:Caloric Restriction; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016014:Linear Models; D015810:Linkage Disequilibrium; D009765:Obesity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D056910:Republic of Korea; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D011518:Proto-Oncogene Proteins; C491119:WNT10B protein, human; D051153:Wnt Proteins