title: [Determination of tomatine in Solanum cathayanum by RP-HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for determination of tomatine in the dried Solanum cathayanum of China Hubei Enshi.
  
  
  METHODS
  The analysis was performed on a YMC-Pack ODS-AA column (4. 6 mm x 150 mm, 5 microm) eluted with acetonitrile and water in gradient mode. The concentration of acetonitrile in the mobile phase changes from 20% to 100% within 60 minutes. The detection wavelength was set 203 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.
  
  
  RESULTS
  The linear relationship of tomatine was determined within the range from 0.1-0.6 g x L(-1) (r = 0.999 7). The average recovery as 98.93% with RSD 1.2%.
  
  
  CONCLUSIONS
  A convenient and reliable method was developed to determine the content of tomatine in the dried S. cathayanum.
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University, Yichang 443002, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Guo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Mingxia
authors.initials: M
authors.lastname: Xie
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D032322:Solanum / Q000737:chemistry; D014053:Tomatine / Q000032:analysis*
chemical_list: D014053:Tomatine