Comprehensive and innovative techniques for liver transplantation in rats: a surgical guide.
OBJECTIVE
To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery.


METHODS
We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.


RESULTS
Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis.


CONCLUSIONS
OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model.
Hori
Tomohide
T
Department of Neuroscience, Mayo Clinic, Birdsall Research Building, Room 323, 4500 San Pablo Road, Jacksonville, Florida, FL 32224, United States. horit@kuhp.kyoto-u.ac.jp
Nguyen
Justin H
JH
Zhao
Xiangdong
X
Ogura
Yasuhiro
Y
Hata
Toshiyuki
T
Yagi
Shintaro
S
Chen
Feng
F
Baine
Ann-Marie T
AM
Ohashi
Norifumi
N
Eckman
Christopher B
CB
Herdt
Aimee R
AR
Egawa
Hiroto
H
Takada
Yasutsugu
Y
Oike
Fumitaka
F
Sakamoto
Seisuke
S
Kasahara
Mureo
M
Ogawa
Kohei
K
Hata
Koichiro
K
Iida
Taku
T
Yonekawa
Yukihide
Y
Sibulesky
Lena
L
Kuribayashi
Kagemasa
K
Kato
Takuma
T
Saito
Kanako
K
Wang
Linan
L
Torii
Mie
M
Sahara
Naruhiko
N
Kamo
Naoko
N
Sahara
Tomoko
T
Yasutomi
Motohiko
M
Uemoto
Shinji
S
D000818:Animals; D006085:Graft Survival; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000601:surgery; D016031:Liver Transplantation / Q000295:instrumentation / Q000379:methods*; D023421:Models, Animal; D051381:Rats; D011917:Rats, Inbred Lew; D015996:Survival Rate; D016896:Treatment Outcome