[Determination of tractylodinol in different populations of Atractylodes lancea].
OBJECTIVE
To establish an RP-HPLC method for determination of atractylodinol in Ateractylodes lancea and compare the contents of atractylodinol in the herbs of different origins.


METHODS
The samples were separated on an Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (49:51). Flow rate was 1.0 mL x min(-1). The detection wave length was set at 337 nm. Column temperature was 30 degrees C.


RESULTS
The linear range of atractylodinol was 9.12 x 10(-2) -9.12 mg x L(-1) (r = 0.999 9), the average recovery was 97.15%, RSD was 1.5% (n = 5). The contents of atractylodinol were in the range of 0.268-1.213 mg x g(-1) in the samples from different orgins. The contents of atractylodinol in samples growing in Dabieshan mountain were higher than those in Jiangsu province (P < 0.001).


CONCLUSIONS
The established method for determination of atractylodinol is accurate and reliable, which can be used to evaluate the quality of A. lancea, the contents of atractylodinol in the sample was related with its morphological characteristic and geographic orgin.
Fang
Chengwu
C
Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modernized Chinese Material, Hefei 230031, China. cwfang1961@sina.com
Fan
Min
M
Liu
Shoujin
S
Yu
Nianjun
N
D031174:Atractylodes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results
D009930:Organic Chemicals