title: Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition.
abstract: Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p < 0.05). Evaluation by SEM, TEM, and XRD demonstrated that aerosol deposition facilitated HA particles to form a dense and uniform HA layer in the RT group despite no post-heating. In a rabbit tibia model (n = 3), the ratios of bone implant contact and bone area in the RT group (49.88%, 86.05%) were greater than in the HT-400 group (38.82%, 77.34%) or the control (28.31%, 73.86%). The finding of this study showed that the HA coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant.
authors.affiliation: Functional Ceramics Research Group, Korea Institute of Materials Science, Changwon, Gyeongnam 641-831, Republic of Korea.
authors.forename: Dong-Soo
authors.initials: DS
authors.lastname: Park
authors.affiliation: Dental Research Institute, Seoul National University, Seoul 110-768, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214.
authors.forename: Hyeongil
authors.initials: H
authors.lastname: Kim
authors.affiliation: Calcium Phosphate Research Lab, Department of Biomaterials, College of Dentistry, New York University, New York 10010.
authors.forename: Alex Hung Kuo
authors.initials: AHK
authors.lastname: Chou
authors.affiliation: Functional Ceramics Research Group, Korea Institute of Materials Science, Changwon, Gyeongnam 641-831, Republic of Korea.
authors.forename: Byung-Dong
authors.initials: BD
authors.lastname: Hahn
authors.affiliation: R & D Center, Dentium Co., Ltd., Suwon 443-270, Republic of Korea.
authors.forename: Long-Hao
authors.initials: LH
authors.lastname: Li
authors.affiliation: Dental Research Institute, Seoul National University, Seoul 110-768, Republic of Korea.|Department of Oral and Maxillofacial Surgery, School of Dentistry, Brain Korea 21 2nd Program for Craniomaxillofacial Life Science, Seoul National University, Seoul 110-768, Republic of Korea.
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000592:standards; D015921:Dental Implants; D017886:Durapatite; D006801:Humans; D020007:Implants, Experimental; D008422:Materials Testing; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology; D011817:Rabbits; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D017886:Durapatite; D014025:Titanium