Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product.
In Brazil, polysaccharide-protein conjugate vaccine against Neisseria meningitidis group C (MenCPS-TT) using hydrazine-activated-tetanus toxoid (TT) as a carrier protein has been developed. Because of the toxicity of hydrazine in humans, it is necessary to monitor this substance's process control step during the vaccine production. The electroanalytical methodology was developed and validated for the determination of hydrazine during the process control of MenCPS-TT vaccine production by differential pulse polarography. The reduction potential was -0.95 V in acetone and sulphuric acid solution. The method presented linear range between 30 and 150 microgL(-1)and recovery of 93.5+/-0.8%.
Bastos
Renata Chagas
RC
Biomanguinhos/Oswaldo Cruz Foundation/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
de Carvalho
Juliana Machado
JM
da Silveira
Ivna Alana Freitas Brasileiro
IA
do Couto Jacob
Silvana
S
Leandro
Katia Christina
KC
D006834:Hydrazines / Q000032:analysis*; D022401:Meningococcal Vaccines / Q000138:chemical synthesis*; D011048:Polarography / Q000379:methods; D013745:Tetanus Toxoid / Q000276:immunology
D006834:Hydrazines; D022401:Meningococcal Vaccines; D013745:Tetanus Toxoid; C410218:serogroup C meningococcal conjugate vaccine; C029424:hydrazine