title: Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes.
abstract: Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.
authors.affiliation: Research Centre, Centre hospitalier de l'Université de Montréal (CRCHUM) - Hôpital Notre-Dame, Université de Montréal and Institut du Cancer de Montréal, Montréal, Québec, Canada.
authors.forename: Laïla-Aïcha
authors.initials: LA
authors.lastname: Hanafi
authors.forename: Marilène
authors.initials: M
authors.lastname: Bolduc
authors.forename: Marie-Eve Laliberté
authors.initials: ME
authors.lastname: Gagné
authors.forename: Florent
authors.initials: F
authors.lastname: Dufour
authors.forename: Yves
authors.initials: Y
authors.lastname: Langelier
authors.forename: Mohammed-Rachid
authors.initials: MR
authors.lastname: Boulassel
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Routy
authors.forename: Denis
authors.initials: D
authors.lastname: Leclerc
authors.forename: Réjean
authors.initials: R
authors.lastname: Lapointe
mesh_terms: D000595:Amino Acid Sequence; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology; D019013:CD40 Antigens / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D049109:Cell Proliferation; D003001:Cloning, Molecular; D045142:Cross-Priming; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D017863:Potexvirus / Q000276:immunology*; D015202:Protein Engineering; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D019013:CD40 Antigens; D036022:Capsid Proteins; D000939:Epitopes