title: Extending the chinchilla middle ear epithelial model for mucin gene investigation.
abstract: OBJECTIVE
  To investigate the expression of recently identified human mucin genes in an in vivo model of the chinchilla middle ear epithelium (CMEE).
  
  
  METHODS
  CMEE was harvested, RNA was extracted and primers were designed for RT-PCR to assess for expression of mucin genes Muc6, Muc17 and Muc18. Further sequencing of these genes was also accomplished.
  
  
  RESULTS
  Mucin genes Muc6, Muc17 and Muc18 was assessed and found to be identical to the expression in human and mouse MEE.
  
  
  CONCLUSIONS
  This study further characterizes mucin gene expression in the CMEE and provides additional sequence data for chinchilla middle ear genes. The concordance of this gene expression data to that of both the human and mouse models further demonstrates the utility of this animal model in OM investigations.
authors.affiliation: Division of Pediatric Otolaryngology, Medical College of Wisconsin, Children's Hospital of Wisconsin, 9000 W. Wisconsin Avenue, Milwaukee, WI 53226, USA. kersch@mcw.edu
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Kerschner
authors.forename: P
authors.initials: P
authors.lastname: Khampang
authors.forename: Tina
authors.initials: T
authors.lastname: Samuels
mesh_terms: D000818:Animals; D002682:Chinchilla; D004432:Ear, Middle / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D051379:Mice; D055272:Mucin-6 / Q000235:genetics / Q000378:metabolism; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D055272:Mucin-6; D009077:Mucins