title: Caldilinea tarbellica sp. nov., a filamentous, thermophilic, anaerobic bacterium isolated from a deep hot aquifer in the Aquitaine Basin.
abstract: An anaerobic, thermophilic, filamentous (0.45 × >100 µm) bacterium, designated D1-25-10-4(T), was isolated from a deep hot aquifer in France. Cells were non-motile and Gram-negative. Growth was observed at 43-65 °C (optimum 55 °C), at pH 6.8-7.8 (optimum pH 7.0) and with 0-5 g NaCl l(-1) (optimum 0 g NaCl l(-1)). Strain D1-25-10-4(T) was a chemo-organotroph and fermented ribose, maltose, glucose, galactose, arabinose, fructose, mannose, sucrose, raffinose, xylose, glycerol, fumarate, peptone, starch and xylan. Yeast extract was required for growth. Sulfate, thiosulfate, sulfite, elemental sulfur, nitrate, nitrite and fumarate were not used as terminal electron acceptors. The G+C content of the DNA was 61.9 mol%. The major cellular fatty acids of strain D1-25-10-4(T) were C(17 : 0), C(18 : 0,) C(16 : 0) and iso-C(17 : 0). The closest phylogenetic relative of strain D1-25-10-4(T) was Caldilinea aerophila STL-6-O1(T) (97.9 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain D1-25-10-4(T) and Caldilinea aerophila DSM 14535(T) was 8.7 ± 1 %. On the basis of phylogenetic, genotypic and phenotypic characteristics, strain D1-25-10-4(T) represents a novel species within the genus Caldilinea, class Caldilineae, phylum Chloroflexi, for which the name Caldilinea tarbellica sp. nov. is proposed. The type strain is D1-25-10-4(T) ( = DSM 22659(T)  = JCM 16120(T)).
authors.affiliation: Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.|Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Grégoire
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Malek
authors.initials: M
authors.lastname: Bohli
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Manon
authors.initials: M
authors.lastname: Joseph
authors.affiliation: LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Guasco
authors.affiliation: Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Dubourg
authors.affiliation: Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Cambar
authors.affiliation: LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Michotey
authors.affiliation: LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bonin
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005602:France; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D012965:Sodium Chloride