A low-cost, portable, and quantitative spectral imaging system for application to biological tissues.
The ability of diffuse reflectance spectroscopy to extract quantitative biological composition of tissues has been used to discern tissue types in both pre-clinical and clinical cancer studies. Typically, diffuse reflectance spectroscopy systems are designed for single-point measurements. Clinically, an imaging system would provide valuable spatial information on tissue composition. While it is feasible to build a multiplexed fiber-optic probe based spectral imaging system, these systems suffer from drawbacks with respect to cost and size. To address these we developed a compact and low cost system using a broadband light source with an 8-slot filter wheel for illumination and silicon photodiodes for detection. The spectral imaging system was tested on a set of tissue mimicking liquid phantoms which yielded an optical property extraction accuracy of 6.40 +/- 7.78% for the absorption coefficient (micro(a)) and 11.37 +/- 19.62% for the wavelength-averaged reduced scattering coefficient (micro(s)').
Fu
Henry L
HL
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. henry.fu@duke.edu
Yu
Bing
B
Lo
Justin Y
JY
Palmer
Greg M
GM
Kuech
Thomas F
TF
Ramanujam
Nimmi
N
D003198:Computer Simulation; D021621:Imaging, Three-Dimensional / Q000191:economics* / Q000379:methods*; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D013057:Spectrum Analysis / Q000191:economics* / Q000379:methods*