Expression analysis of Wnt-5a in renal epithelial neoplasms: distinguishing renal oncocytoma from a wide spectrum of renal cell carcinomas.
OBJECTIVE
To study the expression of a novel marker, Wnt-5a, in renal epithelial neoplasms and determine its clinicopathological significance.


METHODS
Immunohistochemical analysis of Wnt-5a was carried out in normal human kidney samples as well as in 123 primary renal epithelial neoplasms including 37 clear cell renal cell carcinomas (RCCs), 24 papillary RCCs (15 type 1 and 9 type 2), 25 chromophobe RCCs, 11 Xp11 translocation carcinomas, 6 mucinous tubular and spindle cell carcinomas, and 20 oncocytomas.


RESULTS
Wnt-5a was expressed in 18.9% (7/37) of clear cell RCCs, 12.5% (3/24) of papillary RCCs, 16% (4/25) of chromophobe RCCs, 18.2% (2/11) of Xp11 translocation carcinomas, 0% (0/6) of mucinous tubular and spindle cell carcinomas, and 100% (20/20) of oncocytomas. There was a significant difference in Wnt-5a immunohistochemistry between renal oncocytoma and the other subtypes of RCC (P < 0.01).


CONCLUSIONS
Our results indicate that Wnt-5a is a potentially useful immunohistochemical marker for the complex differential diagnosis between oncocytoma and other subtypes of RCC and also suggest that Wnt-5a may be a tumor suppressor gene in RCC.
Rao
Qiu
Q
Department of Pathology Clinical School of Medical College of Nanjing University, Nanjing Jinling Hospital, Nanjing, Jiangsu, China.
Liu
Xiao-hong
XH
Zhou
Hang-bo
HB
Ma
Heng-hui
HH
Lu
Zhen-feng
ZF
Zhou
Xiao-jun
XJ
D018249:Adenoma, Oxyphilic / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D051153:Wnt Proteins / Q000032:analysis*; D000071818:Wnt-5a Protein
D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein