title: [Research on callus induction and plantlet regeneration of Prunella vulgaris].
abstract: OBJECTIVE
  To study the conditions of callus induction and plantlet regeneration of Prunella vulgaris.
  
  
  METHODS
  By using the orthogonal experiment design, different explants, sucrose, plant growth substances and their ratio for callus induction and differentiation were optimized.
  
  
  CONCLUSIONS
  The inductivity of leaves was the highest, followed by stems, but callus of the leafstalks could not be induced. The main factor of affecting the formation of callus was 6-BA. The optimal medium for callus induction was MS + 6-BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + 2,4-D 0.5 mg x L(-1) + sucrose 3%. The optimal medium for callus differentiation was 1/2 MS + 6-BA 3.25 mg x L(-1) + NAA 1.25 mg x L(-1) + sucrose 2%. The optimal medium for rooting was MS + IBA 1.0 mg x L(-1) + sucrose 3%.
authors.affiliation: Anhui Key Laboratory of Plant Resources and Biology, School of Life Science, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Li
authors.initials: L
authors.lastname: Chang
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xue
authors.forename: Yuling
authors.initials: Y
authors.lastname: Liu
mesh_terms: D003470:Culture Media / Q000737:chemistry; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D031347:Prunella / Q000187:drug effects* / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects*; D013997:Time Factors
chemical_list: D003470:Culture Media