[Norovirus infection in adults with sporadic gastroenteritis during 2007-2008 in Nanning Municipal].
OBJECTIVE
To investigate the epidemiological characteristic and genotype distribution of norovirus infection among adult patients with sporadic gastroenteritis in Nanning Municipal.


METHODS
The epidemiological data of adult diarrhea patients in outpatient clinics of a hospital in Nanning City between January 2007 and December 2008 were analyzed. A total of 696 fecal specimens were collected from the patients and were screened for the presence of norovirus RNA by real-time reverse transcription-polymerase chain reaction. Some positive specimens were selected randomly and subjected for sequencing and phylogenetic analysis to identify genotypes.


RESULTS
The sporadic norovirus infection in adults occurred preferentially during the cold season, and there was no statistical difference in the detection rate between age groups. 183 (26.30%) of 696 specimens were positive for norovirus RNA. Among them, 180 (98.36%) belonged to genogroup II (G II) and the other 3 (1.64%) belonged to genogroup I (G I). The sequence analysis showed that all 10 G II strains selected randomly belonged to genotype G II4, while 3 GI strains were classified into three different genotypes: G I2, G I4 and G I8.


CONCLUSIONS
The study showed that norovirus was an important etiologic agent of sporadic gastroenteritis among adults in Nanning City. Genotype G II4 was the predominant genotype, and genotype GI existed sporadically.
Tan
Dong-mei
DM
Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, Guangxi Zhuang Autonomous Region, China.
Liu
Wei
W
Deng
Li-li
LL
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D017250:Caliciviridae Infections / Q000150:complications* / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D002947:Cities / Q000453:epidemiology*; D005260:Female; D005759:Gastroenteritis / Q000150:complications*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D017422:Sequence Analysis, DNA; D017678:Sex Distribution; D055815:Young Adult
D012367:RNA, Viral