title: Dual effects of alpha-synuclein on neurotoxicity induced by low dosage of rotenone are dependent on exposure time in dopaminergic neuroblastoma cells.
abstract: This study was designed to investigate the effects of alpha-synuclein on toxicity induced by long-term exposure to relatively low concentrations of rotenone. Compared with the control groups, the inhibition of cell viability which overexpressed alpha-synuclein (SH-SY5Y-Syn) improved after 1 and 2 weeks of rotenone treatment. The complex I activity was greater and the mitochondrial membrane swelling intensity was reduced after 1 and 2 weeks of treatment, which indicated that alpha-synuclein, at least in part, resists the rotenone-induced oxidative stress. The results indicate that alpha-synuclein has a dual effect on toxicity of rotenone according to exposure time in human SH-SY5Y cells.
authors.affiliation: Beijing Institute for Neuroscience, Capital Medical University, The Beijing Center of Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Disease of the Ministry of Education, Beijing, 100069, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Chunli
authors.initials: C
authors.lastname: Duan
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008933:Mitochondrial Swelling / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D012402:Rotenone / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D013997:Time Factors; D014162:Transfection; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D051844:alpha-Synuclein; D012402:Rotenone; D010100:Oxygen; D004298:Dopamine