Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')(2), and streptavidin.
Colloidal gold probes, including protein A-, IgG-F(ab')(2)-, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A, IgG, and streptavidin.
Yokota
Sadaki
S
Section of Functional Morphology, Faculty of Pharmaceutical Science, Nagasaki International University, Nagasaki, Japan.
D017979:Gold Colloid / Q000138:chemical synthesis* / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D010316:Particle Size; D011971:Receptors, Immunologic / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
C052643:F(ab')2 receptor; D017979:Gold Colloid; D007074:Immunoglobulin G; D011971:Receptors, Immunologic; D013205:Staphylococcal Protein A; D019809:Streptavidin