title: Antioxidant and anti-inflammatory activities of bean ( Phaseolus vulgaris L.) hulls.
abstract: Hulls obtained by mechanical abrasive dehulling from four bean cultivars were extracted with two solvents, aqueous (70%) acetone and water, and the extracts evaluated for antioxidant and anti-inflammatory activities in relation to their phenolic contents. Total phenolic content and antioxidant activity of bean hulls, measured using oxygen radical absorbance capacity (ORAC) values, were 6-8-fold those of corresponding whole beans. Aqueous acetone (70%) extracted over twice the amount of total phenolics from hulls that exhibited significantly higher antioxidant and stronger inhibitory effect on both cyclooxygenases, COX-1 and COX-2, than water. Acetone extract of black bean hull exhibited strong COX-1 (IC(50) = 1.2 microg/mL) and COX-2 (IC(50) = 38 microg/mL) inhibitory effects, even outperforming aspirin. Bean hull water extracts were stronger inhibitors of lipoxygenase, 15-LOX, than corresponding acetone extracts. Anti-inflammatory activity of bean hulls was dependent on their phenolic content and antioxidant activity that were significantly affected by cultivar and extracting solvent.
authors.affiliation: Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada. oomahd@agr.gc.ca
authors.forename: B Dave
authors.initials: BD
authors.lastname: Oomah
authors.forename: Amélie
authors.initials: A
authors.lastname: Corbé
authors.forename: Parthiba
authors.initials: P
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D008084:Lipoxygenase / Q000032:analysis; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D027805:Phaseolus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D012756:Sheep
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases