title: Effect of Agaricus blazei Murill on the pulmonary tissue of animals with streptozotocin-induced diabetes.
abstract: The present study was designed to evaluate the oxidative stress as well as the therapeutic effect of Agaricus blazei Muril (A. Blazei) in rats with streptozotocin-induced diabetes. We used 25 Wistar rats, and DM was induced by injecting streptozotocin (70 mg/Kg i.p.). Agaricus blazei Muril was administered daily starting 40 days after disease onset. A. Blazei was tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipoperoxidation (LPO), and superoxide dismutase (SOD), catalase, and glutathione peroxidase activities were measured in the pulmonary tissue, as well as the presence of inducible nitric oxide synthase (iNOS), through immunohistochemistry. An anatomopathologic study was also performed. Phytochemical screening of A. Blazei detected the presence of alkaloids and saponins. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hipoxanthine/xanthine oxidase assays. Pulmonary LPO increased in diabetic animals (0.43 +/- 0.09; P < .001) as compared to the control group (0.18 +/- 0.02), followed by a reduction in the A. Blazei-treated group (0.33 +/- 0.04; P < .05). iNOS was found increased in the lung in diabetic rats and reduced in the A. Blazei-treated group. The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. The A. Blazei treatment effectively reduced the oxidative stress and contributed to tissue recovery.
authors.affiliation: Laboratory of Experimental Hepatology and Physiology, Porto Alegre Clinical Hospital, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fábio Cangeri
authors.initials: FC
authors.lastname: Di Naso
authors.forename: Rodrigo Noronha
authors.initials: RN
authors.lastname: de Mello
authors.forename: Sílvia
authors.initials: S
authors.lastname: Bona
authors.forename: Alexandre Simões
authors.initials: AS
authors.lastname: Dias
authors.forename: Marilene
authors.initials: M
authors.lastname: Porawski
authors.forename: Alexandre de Barros Falcão
authors.initials: Ade B
authors.lastname: Ferraz
authors.forename: Marc François
authors.initials: MF
authors.lastname: Richter
authors.forename: Norma Possa
authors.initials: NP
authors.lastname: Marroni
mesh_terms: D000364:Agaricus / Q000737:chemistry; D000470:Alkaloids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000628:therapy*; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D000470:Alkaloids; D000975:Antioxidants; D012503:Saponins; D014280:Triglycerides; D002784:Cholesterol; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase