title: Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats.
abstract: Propofol, an anesthetic drug, has been shown to exhibit antioxidant and anti-inflammatory properties in vitro and in vivo. Hypercoagulopathy is a common clinical feature of sepsis, but the effects of propofol on the coagulation system in septic conditions are unclear. Using the gel-based comparative proteomic approach, together with Western blot analysis, ELISA, antithrombin III activity assay, and blood coagulation test, the effect of propofol on serum proteomic profiles in endotoxemic rats was examined. We identified that serum platelet factor-4 (PF4), an endogenous pro-coagulant, was up-regulated in LPS-challenged rats (p<0.001). Endotoxemia also resulted in hypercoagulopathy as evidenced by the shortening of thromboplastin time and thrombin time. Administration of propofol attenuated LPS-stimulated PF4 release and partially reversed the effect of LPS on thromboplastin time (p=0.0012) and thrombin time (p=0.0072). We demonstrated that propofol reduces serum levels of PF4 and partially corrects the hypercoagulopathy associated with endotoxemia in rats.
authors.affiliation: Department of Anesthesia, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangzhou 510515, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Sun
authors.forename: William Ka Kei
authors.initials: WK
authors.lastname: Wu
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhong
authors.forename: Yawei
authors.initials: Y
authors.lastname: Liu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Xiao
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Miaoning
authors.initials: M
authors.lastname: Gu
mesh_terms: D000818:Animals; D000990:Antithrombin III / Q000378:metabolism; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D001798:Blood Proteins / Q000378:metabolism; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D019446:Endotoxemia / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010978:Platelet Factor 4 / Q000037:antagonists & inhibitors / Q000097:blood* / Q000187:drug effects; D015742:Propofol / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013918:Thrombin Time
chemical_list: D001798:Blood Proteins; D006993:Hypnotics and Sedatives; D008070:Lipopolysaccharides; D020543:Proteome; D010978:Platelet Factor 4; D000990:Antithrombin III; D015742:Propofol