title: Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material.
abstract: The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance and cytotoxicity of membranes as well as the changes on the secondary structure of silk fibroin were analyzed after undergoing treatment with ethanol. Membranes presented amorphous patterns as determined via X-ray diffraction. The secondary structure of silk fibroin on dense membranes was either random coil (silk I) or beta-sheet (silk II), before and after ethanol treatment, respectively. The sterilized membranes presented no cytotoxicity to endothelial cells during in vitro assays. This fact stresses the material potential to be used in the fabrication of biomaterials, as coatings of cardiovascular devices and as membranes for wound dressing or drug delivery systems.
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Grínia M
authors.initials: GM
authors.lastname: Nogueira
authors.forename: Andrea C D
authors.initials: AC
authors.lastname: Rodas
authors.forename: Carlos A P
authors.initials: CA
authors.lastname: Leite
authors.forename: Carlos
authors.initials: C
authors.lastname: Giles
authors.forename: Olga Z
authors.initials: OZ
authors.lastname: Higa
authors.forename: Bronislaw
authors.initials: B
authors.lastname: Polakiewicz
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D001709:Biotechnology / Q000379:methods*; D016466:CHO Cells; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D055595:Mechanical Phenomena / Q000187:drug effects; D008567:Membranes, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013718:Tensile Strength / Q000187:drug effects; D013818:Thermogravimetry; D014866:Waste Products / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D014866:Waste Products; D000431:Ethanol; D005349:Fibroins