Expression of the Beet necrotic yellow vein virus p25 protein induces hormonal changes and a root branching phenotype in Arabidopsis thaliana.
The RNA-3-encoded p25 protein was previously characterized as one of the major symptom determinants of the Beet necrotic yellow vein virus. Previous analyses reported the influence of the p25 protein in root proliferation phenotype observed in rhizomania disease on infected sugar beets (Beta vulgaris). A transgenic approach was developed, in which the p25 protein was constitutively expressed in Arabidopsis thaliana Columbia (Col-0) ecotype in order to provide new clues as to how the p25 protein might promote alone disease development and symptom expression. Transgenic plants were characterized by Southern blot and independent lines carrying single and multiple copies of the transgene were selected. Mapping of the T-DNA insertion was performed on the monocopy homozygote lines. P25 protein was localized both in the nucleus and in the cytoplasm of epidermal and root cells of transgenic plants. Although A. thaliana was not described as a susceptible host for BNYVV infection, abnormal root branching was observed on p25 protein-expressing A. thaliana plants. Moreover, these transgenic plants were more susceptible than wild-type plants to auxin analog treatment (2,4-D) but more resistant to methyl jasmonate (MeJA), abscisic acid (ABA) and to lesser extend to salicylic acid (SA). Hormonal content assays measuring plant levels of auxin (IAA), jasmonate (JA) and ethylene precursor (ACC) revealed major hormonal changes. Global transcript profiling analyses on roots displayed differential gene expressions that could corroborate root branching phenotype and stress signaling modifications.
Peltier
Claire
C
Institut de biologie moléculaire des plantes, Laboratoire Propre du CNRS (UPR 2357) conventionné avec l'Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.
Schmidlin
Laure
L
Klein
Elodie
E
Taconnat
Ludivine
L
Prinsen
Els
E
Erhardt
Mathieu
M
Heintz
Dimitri
D
Weyens
Guy
G
Lefebvre
Marc
M
Renou
Jean-Pierre
JP
Gilmer
David
D
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D027461:Beta vulgaris / Q000821:virology*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007210:Indoleacetic Acids / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000378:metabolism; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000821:virology; D018517:Plant Roots / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D003517:Cyclopentanes; D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C036483:T-DNA; D014764:Viral Proteins; C011006:jasmonic acid