title: Microbial production of acetoacetate by recombinant Escherichia coli.
abstract: A synthetic pathway consisting of octAB encoding succinyl-CoA:acetoacetate CoA-transferase and phaA encoding beta-ketothiolase from Ralstonia eutropha H16 was constructed in Escherichia coli for extracellular production of acetoacetate. The recombinant E. coli produced 8.2g/l acetoacetate in shake flasks at 37 degrees C in 24h. In a 6-L fermentor, extracellular acetoacetate production was enhanced to 13.1g/l after 30h of fed-batch growth. To our knowledge, this is the first study on microbial production of acetoacetate.
authors.affiliation: Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Life Science, Tsinghua University, Beijing 100084, China.
authors.forename: Die
authors.initials: D
authors.lastname: Hu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Shi
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D000090:Acetoacetates / Q000378:metabolism*; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics*; D005110:Extracellular Space / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D053858:Metabolic Networks and Pathways; D043367:Ralstonia / Q000201:enzymology / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D000090:Acetoacetates; C016635:acetoacetic acid