title: Microfluidic dissolved oxygen gradient generator biochip as a useful tool in bacterial biofilm studies.
abstract: A microfluidic chip for generation of gradients of dissolved oxygen was designed, fabricated and tested. The novel way of active oxygen depletion through a gas permeable membrane was applied. Numerical simulations for generation of O(2) gradients were correlated with measured oxygen concentrations. The developed microsystem was used to study growth patterns of the bacterium Pseudomonas aeruginosa in medium with different oxygen concentrations. The results showed that attachment of Pseudomonas aeruginosa to the substrate changed with oxygen concentration. This demonstrates that the device can be used for studies requiring controlled oxygen levels and for future studies of microaerobic and anaerobic conditions.
authors.affiliation: Technical University of Denmark, Department of Micro- and Nanotechnology, Ørsted Plads, Building 345east, DK-2800, Kgs. Lyngby, Denmark. maciej.skolimowski@nanotech.dtu.dk
authors.forename: Maciej
authors.initials: M
authors.lastname: Skolimowski
authors.forename: Martin Weiss
authors.initials: MW
authors.lastname: Nielsen
authors.forename: Jenny
authors.initials: J
authors.lastname: Emnéus
authors.forename: Søren
authors.initials: S
authors.lastname: Molin
authors.forename: Rafael
authors.initials: R
authors.lastname: Taboryski
authors.forename: Claus
authors.initials: C
authors.lastname: Sternberg
authors.forename: Martin
authors.initials: M
authors.lastname: Dufva
authors.forename: Oliver
authors.initials: O
authors.lastname: Geschke
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015374:Biosensing Techniques / Q000379:methods*; D003198:Computer Simulation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009757:Nylons / Q000737:chemistry; D010100:Oxygen / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D013050:Spectrometry, Fluorescence
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer; D010100:Oxygen