title: Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.
abstract: Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
authors.affiliation: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Tian
authors.forename: Ai-Sheng
authors.initials: AS
authors.lastname: Xiong
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Fu
authors.forename: Hu
authors.initials: H
authors.lastname: Xu
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Zheng
authors.forename: Ri-He
authors.initials: RH
authors.lastname: Peng
authors.forename: Quan-Hong
authors.initials: QH
authors.lastname: Yao
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015870:Gene Expression; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020621:Ochrobactrum anthropi / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D005998:Glycine; D000097797:Glyphosate