HPLC/CE-ESI-TOF-MS methods for the characterization of polyphenols in almond-skin extracts.
We have developed two rapid methods using CE and HPLC coupled to ESI-TOF-MS and both these methods have been compared for the separation and characterization of antioxidant phenolic compounds in almond-skin extract. Under optimum CE-ESI-TOF-MS conditions we achieved the determination of nine compounds from the polar fraction in 35 min. Furthermore, by using the HPLC-ESI-TOF-MS method, a total of 23 compounds corresponding to phenolic acids and the flavonoid family were identified from almond skin in only 9 min. The sensitivity, together with mass accuracy and true isotopic pattern of TOF-MS, allowed the identification of a broad series of known phenolic compounds present in almond-skin extracts using HPLC and CE as separation techniques.
Arráez-Román
David
D
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Fu
Shaoping
S
Sawalha
Saleh M S
SM
Segura-Carretero
Antonio
A
Fernández-Gutiérrez
Alberto
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005419:Flavonoids; D010936:Plant Extracts