title: Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging.
abstract: Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles' concentration. The minimum detected number of labeled cells was around 10(4) by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Ge
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shiying
authors.initials: S
authors.lastname: He
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.forename: Gaojun
authors.initials: G
authors.lastname: Teng
authors.forename: Ning
authors.initials: N
authors.lastname: Gu