title: [Genome sequencing and analysis of the bovine viral diarrhea virus-2 strain JZ05-1 isolated in China].
abstract: Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, which is a widespread problem for beef and dairy herds, and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin, China generated cytopathic effect (CPE) in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced, the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results, the JZ05-1 genome was composed of 12285 nucleotides in length (GenBank accession No. GQ888686), which could be divided into three regions: a 387 nt 5'-untranslated region (UTR), a 11694 nt single large open reading frame encoding a polyprotein, and a 204 nt 3'-UTR. The 5'-UTR and genome sequences were analyzed by sequence alignment and construction of phylogenetic trees. The strain JZ05-1 was classified as BVDV type 2a. The BVDV-2 strain JZ05-1 genome showed high similarity to the p11Q isolated in Canada and the XJ-04 isolated in China, with 90% and 91% identity in nucleotide sequence, respectively. Compared with the similarity within the BVDV-2 genotype (96%), the JZ05-1 had low sequence similarity to other BVDV-2 strains.
authors.affiliation: Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109, China. Sman9@126.com
authors.forename: Qing-chao
authors.initials: QC
authors.lastname: Li
authors.forename: Li-guang
authors.initials: LG
authors.lastname: Miao
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Li
authors.forename: Yan-huan
authors.initials: YH
authors.lastname: Liu
authors.forename: Guang-lei
authors.initials: GL
authors.lastname: Zhang
authors.forename: Jia-mei
authors.initials: JM
authors.lastname: Xiao
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002417:Cattle; D002681:China; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions