Trace analysis of bromate in potato snacks using high-performance liquid chromatography-tandem mass spectrometry.
A simple, sensitive, and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method in the negative-ion electrospray ionization (ESI(-)) mode was validated for the quantitation of bromate (BrO(3)(-)) in potato snacks. Ground snack specimens ( approximately 0.5 g/sample) are spiked with Br(18)O(3)(-), stable-isotope labeled bromate internal standard (IS), and vortexed with a mixture of distilled/deionized water (dd water) and heptane. Subsequently, the specimens are centrifuged, and a small portion of the aqueous extract is isolated, diluted with dd water (1:4), and analyzed by HPLC-MS/MS. The methodology has a quantitation range of 10-1000 ppb, an accuracy of 1.5-7.5%, and a precision of 5.2-13.4% across the concentration range.
Arias
Francisco
F
The Procter and Gamble Company, Global Analytical Capability, Mason, OH 45040, USA. arias.f@pg.com
Li
Lijuan
L
Huggins
Thomas G
TG
Keller
Paul R
PR
Suchanek
Paula M
PM
Wehmeyer
Kenneth R
KR
D001959:Bromates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005503:Food Additives / Q000032:analysis*; D005504:Food Analysis; D011198:Solanum tuberosum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001959:Bromates; D005503:Food Additives