Baccoside A suppresses epileptic-like seizure/convulsion in Caenorhabditis elegans.
The 1 mm long Caenorhabditis elegans is one of the prime research tools to study different human neurodegenerative diseases. We have considered the case in which increase in the surrounding temperature of this multicellular model leads to abnormal bursts of neuronal cells that can be linked to seizure or convulsion. The induction of such seizure/convulsion mechanism was done by gradually increasing the temperature with 1x buffer (100 mM NaCl, 50 mM MgCl(2)) in adult C. elegans. In the present experiment it is demonstrated that Baccoside A can significantly reduce the seizure/convulsion in C. elegans at higher temperatures (26-28+/-1 degrees C). Furthermore, in T-type Ca(2+) channel cca-1 mutant worms, no convulsion was recorded. Our experimental results suggest that plant molecules from Bacopa monnieri may be useful in suppressing the seizure/convulsion in worms.
Pandey
Rakesh
R
Central Institute of Medicinal & Aromatic Plants (CSIR), Lucknow 226015, India.
Gupta
Shipra
S
Tandon
Sudeep
S
Wolkenhauer
Olaf
O
Vera
Julio
J
Gupta
Shailendra K
SK
D000818:Animals; D034681:Bacopa / Q000737:chemistry*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D004195:Disease Models, Animal; D004827:Epilepsy / Q000188:drug therapy; D006358:Hot Temperature; D009474:Neurons / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology; D012640:Seizures / Q000188:drug therapy; D014315:Triterpenes / Q000494:pharmacology
D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes