title: Tumor suppressor QM-like gene from disk abalone (Haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge.
abstract: We describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor QM-like protein, AbQM, in the disk abalone Haliotis discus discus. The full-length cDNA (765-bp) of AbQM was found to consist of a 654-bp ORF coding for a 218 amino acid protein of a 25 kDa molecular mass with a 10.2 isoelectric point. Analysis of AbQM sequence revealed the presence of characteristic motifs, including the ribosomal protein L10 signature, SH3-binding motif and two antibiotic binding sites. Phylogenetic analysis confirmed that AbQM is closely related to other mollusk QM proteins, and altogether they form a mollusk QM protein sub-family which displays evolutionary conservation from yeast to human. Tissue-specific expression and transcriptional regulation of AbQM was analyzed by quantitative real-time PCR in response to bacterial (Vibrio alginolyticus and Vibrio parahemolyticus, Listeria monocytogenes) and viral (viral hemorrhagic septicemia virus, VHSV) challenge. AbQM transcripts were found to be expressed ubiquitously in all examined tissues in a constitutive manner, as similar expression levels were detected in hemocytes, mantle, digestive tract and muscle. Upon bacterial and VHSV challenge, AbQM showed significant up-regulation in gills, but not in hemocytes. Taken together, these findings suggest that AbQM in abalone-like mollusks can respond to and facilitate a defensive effect against pathogenic infection.
authors.affiliation: Korea Ocean Research and Development Institute (KORDI), Ansan, P.O. BOX 29, Seoul 426-744, Republic of Korea.
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Chamilani
authors.initials: C
authors.lastname: Nikapitiya
authors.forename: Ilson
authors.initials: I
authors.lastname: Whang
authors.forename: Yu Cheol
authors.initials: YC
authors.lastname: Kim
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kang
authors.forename: Soo-jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Young-Ung
authors.initials: YU
authors.lastname: Choi
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000276:immunology*; D001483:Base Sequence; D049851:Gastropoda / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000502:physiology*; D010802:Phylogeny; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment
chemical_list: C000630686:RPL10 protein, human; D012269:Ribosomal Proteins