title: Diazotrophic growth of Rhodospirillum rubrum with 2-oxoglutarate as sole carbon source affects regulation of nitrogen metabolism as well as the soluble proteome.
abstract: 2-Oxoglutarate plays a central role as a signal in the regulation of nitrogen metabolism in the phototrophic diazotroph Rhodospirillum rubrum. In order to further study the role of this metabolite, we have constructed an R. rubrum strain that has the capacity to grow on 2-oxoglutarate as sole carbon source, in contrast to wild-type R. rubrum. This strain has the same growth characteristics as wild-type with malate as carbon source, but showed clear metabolic differences when 2-oxoglutarate was used. Among other things, the regulation of nitrogen metabolism is altered, which can be related to different modification profiles of the regulatory PII proteins.
authors.affiliation: Department of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.
authors.forename: Pedro Filipe
authors.initials: PF
authors.lastname: Teixeira
authors.forename: Tiago Toscano
authors.initials: TT
authors.lastname: Selao
authors.forename: Veronika
authors.initials: V
authors.lastname: Henriksson
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Agneta
authors.initials: A
authors.lastname: Norén
authors.forename: Stefan
authors.initials: S
authors.lastname: Nordlund
mesh_terms: D000105:Acetyl Coenzyme A / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002952:Citric Acid Cycle; D003470:Culture Media / Q000737:chemistry; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D020543:Proteome; D012247:Rhodospirillum rubrum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007656:Ketoglutaric Acids; D020543:Proteome; D000105:Acetyl Coenzyme A; D002244:Carbon; D005970:Glutamate Synthase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen