Human metabolites of brevetoxin PbTx-2: Identification and confirmation of structure.
Four metabolites were identified upon incubation of brevetoxin (PbTx-2) with human liver microsomes. Chemical transformation of PbTx-2 confirmed the structures of three known metabolites BTX-B5, PbTx-9 and 41, 43-dihydro-BTX-B5 and a previously unknown metabolite, 41, 43-dihydro-PbTx-2. These metabolites were also observed upon incubation of PbTx-2 with nine human recombinant cytochrome P450s (1A1, 1A2, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5). Cytochrome P450 3A4 produced oxidized metabolites while other CYPs generated the reduced products.
Guo
Fujiang
F
Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA.
An
Tianying
T
Rein
Kathleen S
KS
D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008387:Marine Toxins / Q000737:chemistry / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D035964:Oxocins; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D008387:Marine Toxins; D035964:Oxocins; C051732:Ptychodiscus brevis T2 toxin; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System