Microsatellite marker analysis shows differentiation among Trypanosoma cruzi populations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patients : microsatellite marker analysis and T. cruzi.
To investigate whether Trypanosoma cruzi populations found in chagasic cardiopathic and non-cardiopathic patients are genetically differentiated, three molecular microsatellite markers were analysed. This analysis was also applied to compare T. cruzi samples from peripheral blood or dejections of Triatoma infestans fed on the blood of the same patients. In order to obtain the first objective, analyses of predominant T. cruzi genotypes were conducted using three approaches: a locus-by-locus analysis; a Fisher method across three loci; and analysis of molecular variance by Genepop and Arlequin programs. Only with one locus and on the blood samples was a significant differentiation detected among non-cardiopathic and cardiopathic groups, which was not confirmed by the other two methods. On the contrary, with the three approaches, it was found that T. cruzi clones present in the blood of patients are genetically differentiated from those detected in dejections of T. infestans fed on the same patients. Our results showed that the most frequent lineage both in blood as well as in triatomine dejection samples was TcI. No significant difference in T. cruzi lineage distribution was observed among chagasic cardiopathic and non-cardiopathic patients. The majority of the samples (50-60%) had only one T. cruzi clone (uniclonal) either in blood or dejection samples.
Venegas
Juan
J
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Independencia 1027, Santiago, Chile. jvenega@med.uchile.cl
Miranda
Sandra
S
Coñoepan
William
W
Pîchuantes
Sergio
S
Jercic
María Isabel
MI
González
Christian
C
Gajardo
Marta
M
Apt
Werner
W
Arribada
Arturo
A
Sánchez
Gittith
G
D000818:Animals; D001769:Blood / Q000469:parasitology*; D014355:Chagas Disease / Q000469:parasitology* / Q000473:pathology*; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D016054:DNA, Protozoan