[Study on the contents of flavonoids in Citrus reticulata 'Chachi' from various habitats and different collecting periods].
OBJECTIVE
To determine the contents of hesperidin, nobiletin and tangeretin in Citrus reticulata 'Chachi' from various habitats and different collecting periods (from October to December) and study the dynamic change of three flavonoids constituents.


METHODS
The HPLC method was used for analysis the contents of flavonoids in Citrus reticulata 'Chachi'. The system used a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with gradient mobile phase of acetonitrile-methanol (80:20)-2% acetic acid. The monitoring wavelength was at 283 nm and 330 nm and the column temperature was at 25 degrees C with the flow rate of 1.0 ml/min.


RESULTS
The contents of hesperidin, nobiletin and tangeretin in Citrus reticulata 'Chachi' collecting from various habitats descended gradually with the mature of fruit, especially in nobiletin and tangeretin.


CONCLUSIONS
The method was simple, convenient and can be used to provide some foundation for the quality control of Citrus reticulata 'Chachi'.
Lin
Le-wei
LW
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. linlewei_918@163.com
Jiang
Lin
L
Zheng
Guo-dong
GD
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry* / Q000254:growth & development; D017753:Ecosystem; D047309:Flavones / Q000032:analysis / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D006569:Hesperidin / Q000032:analysis / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D012621:Seasons; D013997:Time Factors
D047309:Flavones; D005419:Flavonoids; C008661:nobiletin; D006569:Hesperidin; C059006:tangeretin