A sensitive choline biosensor with supramolecular architecture.
A sensitive biosensor with supramolecular architecture was designed and implemented here to detect choline. Choline oxidase and horseradish peroxidase were assembled onto the polymer of thiolated beta-cyclodextrin and platinum nanoparticles modified gold electrode through 1-adamantane carboxylic acid coupling. Square wave voltammetry showed that the reduction currents at 0.38 mV had a linear relationship with the logarithms of choline concentrations in the range of 10(-9)-10(-2) M, and the detection limit was down to 0.1 nM. Such biosensor also exhibited excellent selectivity, reproducibility and stability.
Zhang
Zhanxia
Z
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Wang
Juan
J
Wang
Xiaolei
X
Wang
Yong
Y
Yang
Xiurong
X
D015374:Biosensing Techniques; D002794:Choline / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry
D013438:Sulfhydryl Compounds; D047392:beta-Cyclodextrins; D010984:Platinum; D006046:Gold; D002794:Choline