Glycosylation interference on RhoA activation: focus on G-CSF.
Glycosylation of cytokines appears to be responsible for several differences in their activity, and focusing on G-CSF, several divergences between the non-glycosylated G-CSF, Filgrastim, and the glycosylated G-CSF, Lenograstim, have been reported. To verify the role of G-CSF glycosylation in mediating these differences we tested in vitro the effects on the RhoA activation of the different G-CSFs, including deglycosylated Lenograstim. The results showed that Filgrastim induced sustained-RhoA activation while Lenograstim did not do so. Deglycosylated Lenograstim mimicked Filgrastim, resulting in RhoA hyper-activation. These in vitro findings demonstrate that the glycosylation of G-CSF plays a crucial role in RhoA activation.
Mattii
Letizia
L
Department of Human Morphology and Applied Biology, Medical Histology and Embryology Section, University of Pisa, Via Roma 55, 56126 Pisa, Italy. l.mattii@med.unipi.it
Battolla
Barbara
B
Azzarà
Antonio
A
D'Urso
Giuseppina
G
Montali
Umberto
U
Petrini
Mario
M
D000276:Adjuvants, Immunologic / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D000069585:Filgrastim; D006031:Glycosylation; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D000078224:Lenograstim; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000078224:Lenograstim; D020742:rhoA GTP-Binding Protein; D000069585:Filgrastim