Effect of intranasal administration of Lactobacillus pentosus S-PT84 on influenza virus infection in mice.
Lactobacillus pentosus strain S-PT84 isolated from Kyoto pickles enhances splenic natural killer (NK) cell activity and exhibit anti-allergic effects by modulating the Th1/Th2 (T-helper1/T-helper2) balance. In the present study, we investigated whether the immune response could be activated by intranasal administration of S-PT84 in the respiratory immune system and protected against influenza virus infection in mice. When BALB/c mice received intranasal administration of S-PT84 once daily for 3 consecutive days, S-PT84 strongly induced interleukin-12 (IL-12) and gamma interferon (IFN-gamma) production in mediastinal lymph node (MLN) cells. At intranasal infection with influenza virus PR8 (a mouse-adapted H1N1 strain) after S-PT84 treatment, the survival rates of mice improved in a dose-dependent manner, and the titer of influenza virus in bronchoalveolar lavage fluids (BALF) was significantly decreased by S-PT84 administration. Production of IL-12 and alpha-interferon (IFN-alpha) in BALF were significantly higher in mice treated with S-PT84 compared to the control mice. Lung NK activity was also significantly augmented in S-PT84-treated mice. These results suggested that the L. pentosus strain S-PT84 showed inhibitory activity against influenza virus infection.
Izumo
Takayuki
T
Institute for Health Care Science, Suntory Wellness Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan. Takayuki_Izumo@suntory.co.jp
Maekawa
Toshihiro
T
Ida
Masayuki
M
Noguchi
Akane
A
Kitagawa
Yoshinori
Y
Shibata
Hiroshi
H
Yasui
Hisako
H
Kiso
Yoshinobu
Y
D000281:Administration, Intranasal; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype; D007371:Interferon-gamma / Q000032:analysis; D018664:Interleukin-12 / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D007778:Lactobacillus / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology / Q000821:virology; D008482:Mediastinum / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000628:therapy*; D018417:Th1 Cells / Q000276:immunology*
D018664:Interleukin-12; D007371:Interferon-gamma