An analytical method for prohexadione in Chinese cabbage and apple.
A residual determination method as a regulatory residue method was developed using HPLC-UVD for prohexadione residues in Chinese cabbage (Brassica pekinensis) and apple (Malus domestica). The developed method consisted of solid-liquid extraction with acidic acetonitrile and ion-suppression liquid-liquid partitioning, followed by anion exchange cartridge cleanup. The limits of detection and quantitation for the method were 0.005 and 0.02 mg/kg, respectively. The method gave good linearity in the range of 0.02-2.5 mg/kg. Accuracy and precision ranged from 84.1 to 94.1% and from 2.4 to 6.9%, respectively. Additionally, the confirmative conditions of LC-MS/MS for prohexadione were set in negative electrospray ionization mode with transitions of m/z 211.4 → 167.5 and m/z 211.4 → 123.5 in the selected reaction monitoring mode. The applicability of the method was demonstrated by analyzing real samples collected from local markets in Seoul, Republic of Korea. This developed method fully deserves consideration in accordance with its sensitivity, accuracy and precision required for residue analysis of prohexadione in Chinese cabbages and apples.
Choi
Jeong-Heui
JH
Food Chemical Residues Division, Korea Food and Drug Administration, 194 Tongilro, Eunpyeong-gu, Seoul, Republic of Korea.
Yoon
Hae-Jung
HJ
Do
Jung-Ah
JA
Park
Yong-Chun
YC
Kim
Jae Hun
JH
Choi
Dongmi
D
D000097:Acetonitriles; D001937:Brassica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007656:Ketoglutaric Acids / Q000032:analysis*; D016014:Linear Models; D027845:Malus / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D056910:Republic of Korea; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
D000097:Acetonitriles; D007656:Ketoglutaric Acids; D010573:Pesticide Residues; C479018:prohexadione-Ca; C032159:acetonitrile