[Combined estrogenic activity of soybean extract used in a dietary supplement and ethinyl estradiol].
We examined the combined estrogenic activity of soybean extract used in a dietary supplement and ethinyl estradiol (EE) contained in an oral contraceptive. Olive oil (control), soybean extract (0.0036 or 0.36 g/kg corresponding to doses of total isoflavone of 0.83 or 83 mg/kg respectively), EE (1 or 10 microg/kg), and soybean extract+EE were administered to ovariectomized CD-1 mice by oral gavage for 4 consecutive days. Soybean extract (0.0036 or 0.36 g/kg) and EE (1 microg/kg) did not increase the relative uterine weight. The relative uterine weight of the soybean extract (0.0036 or 0.36 g/kg)+EE (10 microg/kg) group was significantly higher than that of the control. The relative uterine weight of the soybean extract (0.36 g/kg)+EE (10 microg/kg) group was also significantly higher than that of the EE (10 microg/kg) group. Soybean extract showed estrogenic activity for human estrogen receptor (hER)-alpha and -beta. Coadministration of EE with soybean extract increased the estrogenic activity for hER-alpha and -beta.
Kawazoe
Sadahiro
S
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Kyoto, Japan.
Naka
Kazuna
K
Ishibashi
Hiroshi
H
Obara
Tomomi
T
Arizono
Koji
K
Hashimoto
Kaori
K
Hojo
Yasuji
Y
Suzuki
Takashi
T
D000818:Animals; D003276:Contraceptives, Oral / Q000032:analysis; D019587:Dietary Supplements; D047628:Estrogen Receptor alpha / Q000187:drug effects; D047629:Estrogen Receptor beta / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000008:administration & dosage* / Q000494:pharmacology*; D005260:Female; D007529:Isoflavones / Q000032:analysis; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D014599:Uterus / Q000187:drug effects
D003276:Contraceptives, Oral; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D007529:Isoflavones; D010936:Plant Extracts; D004997:Ethinyl Estradiol; C065753:isoflavanone