Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed Fructus corni on human L02 hepatocytes.
OBJECTIVE
To determine the mode of action of 5-hydroxymethylfurfural (5-HMF) extracted from wine-processed Fructus corni on hepatoprotective activities, the effects of 5-HMF on H(2)O(2)-induced human L02 hepatocytes injury was examined. MTHODS: Hepatocytes L02 injured by H(2)O(2) was treated by 5-HMF. The morphological changes of the cells were observed under inverted phase-contrast, fluorescence, and transmission electron microscopy and the activities of caspase-9 and caspase-3 were tested by enzyme-linked immunosorbent detector.


RESULTS
It revealed that 5-HMF improved the morphology of H(2)O(2)-treated human L02 hepatocytes, and also inhibited the level of caspase-9 and caspase-3 of them.


CONCLUSIONS
These results suggested a morphological hepatocyte protective effect and the anti-apoptosis mechanism by 5-HMF.
Wang
Ming-Yan
MY
Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China.
Zhao
Feng-Ming
FM
Peng
Hai-Yan
HY
Lou
Cheng-Hua
CH
Li
Yu
Y
Ding
Xia
X
Yu
Xiao-Yi
XY
Yang
Guang-Ming
GM
Xu
Dong-Qing
DQ
Jiang
Li-Hua
LH
Zhang
Xu
X
Ye
Li-Hong
LH
Cai
Bao-Chang
BC
D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects*; D029768:Cornus; D019610:Cytoprotection; D004797:Enzyme-Linked Immunosorbent Assay; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D010936:Plant Extracts / Q000494:pharmacology*; D014920:Wine
D010936:Plant Extracts; C008046:5-hydroxymethylfurfural; D006861:Hydrogen Peroxide; D005662:Furaldehyde; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9