Cellular receptors, differentiation and endocytosis requirements are key factors for type I IFN response by human epithelial, conventional and plasmacytoid dendritic infected cells by measles virus.
While the antiviral response during measles virus (MeV) infection is documented, the contribution of the hosting cell type to the type I interferon (IFN-alpha/beta) response is still not clearly established. Here, we report that a signature heterogeneity of the IFN-alpha/beta response according to the cell type. The MeV tropism dictated by the expression of appropriate cellular receptor appeared to be crucial for epithelial cells. For conventional DCs (cDCs), the maturation state played a prominent role. In response to both wild type MeV isolates and laboratory/vaccine strains, immature cDCs produced higher levels of IFN-alpha than mature cDCs, despite the reduced expression levels of both CD46 and CD150 receptors by the former ones. While in epithelial cells and cDCs the MeV transcription was required to activate the IFN-alpha/beta response, plasmacytoid DCs (pDCs) rapidly produced large amounts of IFN-alpha mostly independently of the viral infection cycle. This argues for a significant contribution of pDCs in response to MeV infection and/or vaccination.
Duhen
Thomas
T
Université de Lyon, INSERM U851, 21 Avenue Tony Garnier, Lyon, France.
Herschke
Florence
F
Azocar
Olga
O
Druelle
Johan
J
Plumet
Sébastien
S
Delprat
Chistine
C
Schicklin
Stéphane
S
Wild
T Fabian
TF
Rabourdin-Combe
Chantal
C
Gerlier
Denis
D
Valentin
Hélène
H
D015703:Antigens, CD / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology; D004705:Endocytosis; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008457:Measles / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D051916:Membrane Cofactor Protein / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D011991:Receptors, Virus / Q000276:immunology*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D056189:Viral Tropism
D015703:Antigens, CD; D007370:Interferon Type I; D051916:Membrane Cofactor Protein; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1