title: Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4.
abstract: This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (∼1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
authors.affiliation: College of Systems Engineering and Science, Department of Bioscience and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama-shi, Saitama 337-8570 Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Iwata
authors.forename: Azlin
authors.initials: A
authors.lastname: Azlan
authors.forename: Hironori
authors.initials: H
authors.lastname: Yamakawa
authors.forename: Toshio
authors.initials: T
authors.lastname: Omori
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053528:Lysobacter / Q000235:genetics / Q000378:metabolism*; D009586:Nitrogen Fixation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
chemical_list: D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D000641:Ammonia; D005947:Glucose