title: [Effect of phosphatase PHLPP1 gene transfer on the proliferation of human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To investigate the constituent expression of PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1) in human umbilical vein endothelial cells (HUVECs) and the effect of PHLPP1 gene transfer on the proliferation of the cells in vitro.
  
  
  METHODS
  Cultured HUVECs were transfected with pcDNA3-GFP or pcDNA3HA-PHLPP1 via lipofectamine 2000. The cell proliferation ability was determined by cell counting and MTT colorimetric assay, and Western blotting was used to detect the protein expression of PHLPP1 in the cells.
  
  
  RESULTS
  No PHLPP1 protein was detected in the non-transfected cells or pcDNA3-GFP-transfected cells. pcDNA3HA-PHLPP1 gene transfection significantly increased PHLPP1 expression in the HUVECs (P<0.01), but the cell proliferation status remained unchanged (P>0.05). The absorbance of the cells measured by MTT assay was 0.134-/+0.0152, 0133-/+0.014 and 0.137-/+0.016, with cell counts of (8.293-/+0.962)x10(5), (7.937-/+0.101)x10(5) and (8.127-/+0.112)x10(5), respectively, showing no significant differences between the 3 groups (P>0.05).
  
  
  CONCLUSIONS
  Phosphatase PHLPP1 may not be the most important signal protein in the regulation of HUVEC proliferation.
authors.affiliation: Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China. WXL301@yahoo.com
authors.forename: Xing-li
authors.initials: XL
authors.lastname: Wu
authors.forename: Ding-you
authors.initials: DY
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Ai-jia
authors.initials: AJ
authors.lastname: Shang
authors.forename: Cai-yi
authors.initials: CY
authors.lastname: Lu
authors.forename: Hui-bin
authors.initials: HB
authors.lastname: Xu
authors.forename: Shi-wen
authors.initials: SW
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D018014:Gene Transfer Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D014162:Transfection
chemical_list: D009687:Nuclear Proteins; C400601:PHLPP1 protein, human; D010749:Phosphoprotein Phosphatases