(-)-Epigallocatechin-3-O-gallate (EGCG) reverses caffeine-induced anxiogenic-like effects.
This study was designed to determine whether (-)-epigallocatethin-3-O-gallate (EGCG) could reverse caffeine-induced anxiogenic-like effects in animals. In mice, EGCG antagonized the caffeine-induced reduction in both the open arm entry number and time-spent in open arm on elevated plus-maze. In addition, EGCG also antagonized the caffeine-induced reduction in both the central zone distance and central zone time-spent on an open field apparatus, respectively. Electroencephalogram (EEG) was recorded from the rat anterior cerebral cortex. Caffeine increased the power density-ratios of fast (FW: 8.00-20.00 Hz) and slow (SW: 0.75-8.00 Hz) frequency spectrum bands in these EEG recordings. However, EGCG reduced the caffeine-induced increase of FW/SW ratios. Thus, EGCG reverses caffeine-induced anxiogenic-like effects. We also provide additional evidence that the EEG FW/SW (or SW/FW) ratios can be a useful tool for the prediction of anxiogenic and/or anxiolytic effects in an animal model.
Park
Kwang-Soon
KS
College of Pharmacy and CBITRC, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Oh
Ju Hoon
JH
Yoo
Hwan-Soo
HS
Lee
Yong-Moon
YM
Lee
Myung-Koo
MK
Hong
Jin Tae
JT
Oh
Ki-Wan
KW
D000818:Animals; D001007:Anxiety / Q000139:chemically induced* / Q000188:drug therapy*; D002110:Caffeine / Q000009:adverse effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000697:Central Nervous System Stimulants / Q000009:adverse effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography / Q000379:methods; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013057:Spectrum Analysis
D000697:Central Nervous System Stimulants; D018696:Neuroprotective Agents; D002110:Caffeine; D002392:Catechin; C045651:epigallocatechin gallate