Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media.
A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol:water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol:water (80:20, v/v) for 90 min, drying with N(2) and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier.
Medina
Angel
A
Applied Mycology Group, Cranfield Health, Vincent Building, Cranfield University, College Road, Cranfield, Bedfordshire, MK43 0AL, UK. a.medinavaya@cranfield.ac.uk
Valle-Algarra
Francisco M
FM
Jiménez
Misericordia
M
Magan
Naresh
N
D053000:Analytic Sample Preparation Methods; D018554:Avena / Q000737:chemistry*; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry*; D003890:Desiccation; D005670:Fusarium / Q000166:cytology; D012015:Reference Standards; D012997:Solvents; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000032:analysis; D013997:Time Factors
D003470:Culture Media; D012997:Solvents; D013605:T-2 Toxin; C012351:HT-2 toxin