Specific and efficient anti-Aβ42 antibodies induced by sixteen tandem repeats of Aβ9.
To reduce the risk of an adverse T cell-mediated immune response to Aβ42, three (Aβ9)16-based recombinant immunogens were designed to immunize mice. Compared with Aβ42, they elicited significantly stronger anti-Aβ42 antibody responses and mainly resulted in IgG1 and IgG2b anti-Aβ42 antibodies. These (Aβ9)16-induced antibodies exhibited stronger abilities not only to inhibit Aβ42 aggregation and to disassemble Aβ42 aggregation, but also to inhibit and neutralize Aβ42-induced cytotoxicity in vitro though they showed higher specificities to Aβ42 monomers and Aβ42 oligomers. These results suggested that (Aβ9)16 was a promising candidate for the safe and effective primary immunogen against Aβ42.
Cui
Lili
L
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, 2519 Jie-Fang Road, Changchun 130021, PR China.
Huang
Xuemei
X
Wang
Jiapeng
J
Zhang
Yingjiu
Y
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D000918:Antibody Specificity / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D011897:Random Allocation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000633:toxicity; D020080:Tandem Repeat Sequences / Q000235:genetics / Q000276:immunology*
D016229:Amyloid beta-Peptides; D001323:Autoantibodies; D010446:Peptide Fragments; D011994:Recombinant Proteins; C549047:amyloid beta-peptide (9-42)