title: Identification of bovine CD52-like molecule by monoclonal antibody IVA-543: distribution of CD52-like molecule in the bull genital tract.
abstract: The bovine maturation-associated sperm membrane antigen CD52-like molecule has been analysed using a mouse anti-sperm monoclonal antibody developed against bull spermatozoa. The antigen recognised by monoclonal antibody IVA-543 was detected on blood mononuclear cells (including lymphocytes and monocytes) and on a minor population of polymorphonuclear leukocytes. The bovine CD52-like molecule is secreted by the epididymal epithelium and then it is inserted into the sperm membrane during the epididymal transport in the distal part of epididymis. The CD52-like molecule was absent from spermatozoa derived from testes, and the highest proportion of IVA-543-reactive sperm was observed in the cauda epididymis (91.6%). This study has shown that the new molecule identified on bovine cells has properties analogous to those previously described for CD52 molecules in man, mouse, rat, monkey, and dog.
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic. Katarina.Fabryova@savba.sk
authors.forename: K
authors.initials: K
authors.lastname: Michalková
authors.forename: M
authors.initials: M
authors.lastname: Simon
authors.forename: J
authors.initials: J
authors.lastname: Antalíková
authors.forename: J
authors.initials: J
authors.lastname: Klíma
authors.forename: L'
authors.initials: L
authors.lastname: Horovská
authors.forename: J
authors.initials: J
authors.lastname: Jankovicová
authors.forename: S
authors.initials: S
authors.lastname: Hluchý
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D000918:Antibody Specificity; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D001773:Blood Cells / Q000378:metabolism; D000074301:CD52 Antigen; D002417:Cattle / Q000378:metabolism*; D004285:Dogs; D004542:Ejaculation; D005434:Flow Cytometry; D005837:Genitalia, Male / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017385:Sequence Homology; D013094:Spermatozoa / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; C000613862:CD52 protein, human; C000613864:Cd52 protein, rat; D006023:Glycoproteins