title: Differential expression of the mango alcohol dehydrogenase gene family during ripening.
abstract: Alcohol dehydrogenases play an important role during fruit ripening and aroma production. Three full-length cDNAs (MiAdh1, 2 and 3) encoding alcohol dehydrogenases were obtained from mango fruit pulp using RT-PCR approaches. All three members displayed strong homology in the coding region when compared at the protein and nucleotide levels, however showed variations in untranslated regions. Expression patterns of these ADHs were different during fruit development and ripening. MiADH1 and MiADH2 transcripts accumulated at the onset of ripening in mango fruit whereas MiADH3 accumulated during early development of fruit. Expression analysis also indicated that mango ADHs were responsive to ethylene but regulated differently by ABA. MiADH1 was induced by ABA treatment whereas MiADH2 transcript was negatively regulated by ABA. MiADH3 did not respond to ABA in ripening fruit. Differences in substrate specificity for NADH and NADPH were also observed between the three enzymes. Total ADH enzyme activity correlated positively with increased transcript levels at the initiation of ripening.
authors.affiliation: Plant Gene Expression Lab., National Botanical Research Institute, Lucknow 226 001, India.
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Singh
authors.forename: Vidhu A
authors.initials: VA
authors.lastname: Sane
authors.forename: Aparna
authors.initials: A
authors.lastname: Misra
authors.forename: Sharique A
authors.initials: SA
authors.lastname: Ali
authors.forename: Pravendra
authors.initials: P
authors.lastname: Nath
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D031022:Mangifera / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D000426:Alcohol Dehydrogenase