title: Analysis of gelatin plasma substitutes in blood based on detection of hydroxyproline derivatives.
abstract: The gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Guifeng
authors.initials: G
authors.lastname: Zhanga
authors.forename: Suping
authors.initials: S
authors.lastname: Li
authors.forename: Yinjue
authors.initials: Y
authors.lastname: Wang
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000378:metabolism; D001810:Blood Volume / Q000187:drug effects; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives / Q000097:blood; D005780:Gelatin / Q000009:adverse effects / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D006207:Half-Life; D006470:Hemorrhage / Q000097:blood; D006868:Hydrolysis; D006909:Hydroxyproline / Q000097:blood* / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000737:chemistry; D039823:Iridoids; D008297:Male; D010952:Plasma Substitutes / Q000009:adverse effects / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D011817:Rabbits; D013387:Succinic Anhydrides / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D010952:Plasma Substitutes; D013387:Succinic Anhydrides; C031801:succinic anhydride; D005780:Gelatin; C007834:genipin; D004139:Dinitrofluorobenzene; D006909:Hydroxyproline