Preparation of interconnected porous chitosan scaffolds by sodium acetate particulate leaching.
For tissue-engineering applications, a 3D porous chitosan scaffold was simply prepared from a mixture of acidic chitosan solution and sodium acetate particles as the porogen by a salt-leaching method. Differences in the porous structure in terms of pore morphology and interconnectivity between the salt-leached chitosan scaffold and phase-separated scaffold as the control were examined by using scanning electron microscopy, protein release and enzymatic degradation tests. A fibroblast (NIH-3T3) cell culture was performed for cell affinity evaluation. The chitosan scaffold prepared by salt-leaching showed good interconnectivity and improved mechanical properties. Furthermore, the chitosan scaffolds showed a high initial cell adhesion after 4 h cell culture and increased cell proliferation than the control. Thus, salt-leached chitosan scaffolds can be used for various tissue-engineering applications.
Lim
Jin Ik
JI
a National Core Research Center for Nanomedical Technology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, South Korea.
Lee
Yong-Keun
YK
Shin
Jeon-Soo
JS
Lim
Kook-Jin
KJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D048271:Chitosan / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D064347:Microscopy, Electrochemical, Scanning; D041681:NIH 3T3 Cells; D016062:Porosity; D019346:Sodium Acetate / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D019346:Sodium Acetate; D048271:Chitosan