title: Mechanical distortion of protein receptor decreases the lifetime of a receptor-ligand bond.
abstract: Substantial experimental evidence indicates that the mechanical force applied to pull apart non-covalent molecular bonds (such as receptor-ligand pairs) can significantly decrease the bond lifetime. This evidence is often generated in single-molecule experiments that are designed to specifically test effects of pulling forces. However, the effect of compressive forces on the lifetime of receptor-ligand bonds remains largely unexplored. Here we extend the common usage of the atomic force microscopy technique to study whether compressive forces applied to bound streptavidin-biotin species can significantly accelerate the rate of dissociation. Presented experimental data indicate that compressive forces can substantially decrease the lifetime of the molecular bond. Surprisingly, the efficiency of accelerating dissociation by compressive forces sometimes exceeds the enhancement of the dissociation rate measured in pulling experiments, indicating that compressive forces applied to the bound species might be efficiently used to control the lifetime of adhesion bonds.
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Senli
authors.initials: S
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Nimit
authors.initials: N
authors.lastname: Lad
authors.forename: Chad
authors.initials: C
authors.lastname: Ray
authors.forename: Boris B
authors.initials: BB
authors.lastname: Akhremitchev
mesh_terms: D008024:Ligands; D018625:Microscopy, Atomic Force; D011506:Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D008024:Ligands; D011506:Proteins; D011956:Receptors, Cell Surface