The mRNA expression of cytochrome P450 isoforms in human gastric tissue.
OBJECTIVE
Human Cytochrome P450 (CYP) comprises a multigene family of microsomal enzymes that metabolize a wide variety of xenobiotics, including drugs and carcinogens. Although the a number of CYP enzymes were also detected in epithelial cells along the gastrointestinal tract, little is known about the expression of CYP genes in gastric tissue.


METHODS
In this study, the expression patterns of CYP isoforms was investigated in a total of 14 antral biopsy tissues obtained from the patients with either chronic gastritis (n = 6) or cancer (n = 8) by gene-specific real-time reverse transcriptase -PCR analyses. We employed primer sets specific for CYPs -1A1, -1A2, -2A6, -2B6, -2C, -2D6, -2E1, and -3A5.


RESULTS
Among the isoforms CYP1A1, CYP2C and CYP2D6 gave rise to detectable mRNAs in all 14 gastric tissues while the mRNAs for the other CYPs were detected in some of the tissues. The expression patterns were compared to clinical parameters. There were no significant differences in the parameters between the two groups; however the mRNA expression of CYP2A6 was significantly higher in women than man (p < 0.05).


CONCLUSIONS
Our data suggests that the CYP isoforms were independently expressed with respect to the pathological status in human gastric tissue.
Canturk
Pakize
P
Department of Pharmaceutical Biotechnology, School of Pharmacy, Ege University, Izmir.
Caner
Vildan
V
Oruc
Nevin
N
Akarca
Ulus Salih
US
Tepeli
Emre
E
Cetin
Ozan G
OG
Zencir
Sevil
S
Topcu
Zeki
Z
D000328:Adult; D000368:Aged; D001706:Biopsy; D016009:Chi-Square Distribution; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005260:Female; D005756:Gastritis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020033:Protein Isoforms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism*
D017931:DNA Primers; D020033:Protein Isoforms; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System