Quantitative SLM-based Differential Interference Contrast imaging.
We describe the implementation of quantitative Differential Interference Contrast (DIC) Microscopy using a spatial light modulator (SLM) as a flexible Fourier filter in the optical path. The experimental arrangement allows for the all-electronic acquisition of multiple phase shifted DIC-images at video rates which are analyzed to yield the optical path length variation of the sample. The resolution of the technique is analyzed by retrieving the phase profiles of polystyrene spheres in immersion oil, and the method is then applied for quantitative imaging of biological samples. By reprogramming the diffractive structure displayed at the SLM it is possible to record the whole set of phase shifted DIC images simultaneously in different areas of the same camera chip. This allows for quantitative snap-shot imaging of a sample, which has applications for the investigation of dynamic processes.
McIntyre
Timothy J
TJ
School of Mathematics and Physics, The University of Queensland, Brisbane, Australia. t.mcintyre@uq.edu.au
Maurer
Christian
C
Fassl
Stephanie
S
Khan
Saranjam
S
Bernet
Stefan
S
Ritsch-Marte
Monika
M
D048430:Cell Shape; D002875:Chromosomes / Q000648:ultrastructure; D004867:Equipment Design; D004912:Erythrocytes / Q000166:cytology; D005583:Fourier Analysis; D006696:Holography / Q000379:methods; D008027:Light; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D008962:Models, Theoretical; D011137:Polystyrenes; D012984:Software
D011137:Polystyrenes