title: [Evaluation on running status of Chinese Polio Laboratories Network in 2008].
abstract: OBJECTIVE
  In order to evaluate the running status and provide the laboratory data for maintaining polio-free status in China, the virology surveillance database of Chinese Polio Laboratories Network (not include Hong Kong, Macao, and Taiwan)in 2008 were analyzed.
  
  
  METHODS
  The case investigation data of Acute Flaccid Paralysis(AFP)cases reported by 31 provinces (municipal, autonomous regions) through EPI surveillance information management system and the database of National Polio Laboratory (NPL) were analyzed, and the indicators of running status of Chinese Polio Laboratories Network were evaluated.
  
  
  RESULTS
  10,116 stool samples were collected from 5116 AFP cases by Chinese Polio Laboratories Network in 2008, and viral isolation and identification of all stool samples were done according to 4th World Health Organization (WHO) Polio Laboratory Manual. The rate of viral isolation and identification performed within 28d was 94.9%. 189 polioviruses (PV) and 597 of non-polio enteroviruses (NPEV) were isolated from AFP cases, the isolatien rates were 3.72% and 11.74% respectively. 251 polio positive isolates were sent to NPL from 31 provincial polio laboratories. There were 318 single serotype PVs were performed VPI sequencing. And no wild polioviruses and Vaccine-derived Polioviruses (VDPVs) were found in 2008. NPL passed the proficiency test and got full accreditation for on-site review by WHO experts in 2008. All 31 provincial Polio laboratories passed the proficiency test with the same panel as NPL, and 13 provincial Polio laboratories joined and passed the on-site review by WHO experts.
  
  
  CONCLUSIONS
  The running status of Chinese Polio Laboratories Network was good, polio-free status was maintained in China in 2008. The Chinese polio laboratories network running is normaly, the laboratory surveillance system was sensitive and laboratory data were provided for maintaining the polio-free status in China.
authors.affiliation: World Health Organization Western Pacific Region Regional Polio Laboratory, State Key Laboratory for Molecular Virology & Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Shuang-li
authors.initials: SL
authors.lastname: Zhu
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D016208:Databases, Factual; D006801:Humans; D007753:Laboratories / Q000592:standards / Q000706:statistics & numerical data*; D016366:Open Reading Frames / Q000235:genetics; D010264:Paraplegia / Q000453:epidemiology / Q000821:virology; D011051:Poliomyelitis / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D023321:Poliovirus Vaccines; D015397:Program Evaluation; D011786:Quality Control; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D023321:Poliovirus Vaccines; C442540:VP1 protein, Poliovirus