Antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (Raphanus sativus var. niger, Brassicaceae).
The antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. The extract appeared to form a complex with Fe(3+) or Cu(2+). It displayed a concentration-dependant reducing power (1.16OD(700 nm) at a concentration of 4mM) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with IC(50)=1.74 +/- 0.03 mM). It could promote the cleavage of plasmid DNA with Cu(II)/H(2)O(2) or Cu(II) alone. This DNA damage could be inhibited by horseradish peroxidase, catalase, and EDTA, respectively. The extract also showed growth inhibition of Bel-7402 cells at lower concentration. The results suggested that the formation of reactive oxygen species might be involved in the mechanism of DNA damage. The acylated pelargonidin derivatives extracted from red radish could act as antioxidant and pro-oxidant and their antioxidant and pro-oxidant properties were relative to the reaction conditions. It might provide novel antioxidant and anticarcinogenic agents.
Wang
Lin-Song
LS
College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China. wls@henannu.edu.cn
Sun
Xiang-Dong
XD
Cao
Ying
Y
Wang
Li
L
Li
Fang-Jun
FJ
Wang
Yi-Fan
YF
D000215:Acylation; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D052160:Benzothiazoles / Q000737:chemistry; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000187:drug effects; D031224:Raphanus / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles
D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D004396:Coloring Agents; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013451:Sulfonic Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C066957:pelargonidin; C022616:thiazolyl blue