Online monitoring of cellular metabolism in the MCF-7 carcinoma cell line treated with phytoestrogen extracts.
BACKGROUND
Phytoestrogens are naturally occurring, plant-derived, nonsteroidal phytochemicals with anticarcinogenic potential. The aim of this study was to isolate phytoestrogens from the flax root of Linum usitatissimum and to test their effect on cellular metabolism in the human mammalian carcinoma cell line MCF-7 using the Bionas 2500 analysis system.


METHODS
Metabolically relevant parameters such as acidification, oxygen consumption and cell adhesion were registered continuously over 8 and 24 hours on six sensor chips in parallel at different concentrations of flax root extracts.


RESULTS
The extracts from flax roots of L. usitatissimum reduced extracellular acidification, respiration and adhesion in a concentration-dependent manner.


CONCLUSIONS
The Bionas 2500 analysis system allows multiparametric online monitoring of cellular processes and can be used to detect the mode of action of anticarcinogenic compounds in cellular metabolism.
Abarzua
S
S
Department Biological Sciences, University of Rostock, Institute of Biological Sciences, Rostock, Germany. Sibylle.abarzua@uni-rostock.de
Drechsler
S
S
Fischer
K
K
Pietschmann
N
N
Stapel
J
J
Duda
S
S
Richter
D-U
DU
Ehret
R
R
Piechulla
B
B
Briese
V
V
D015374:Biosensing Techniques; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods*; D004867:Equipment Design; D019597:Flax / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008660:Metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010101:Oxygen Consumption; D048789:Phytoestrogens / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
D048789:Phytoestrogens; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue