Long-term histological and behavioural characterisation of a collagenase-induced model of intracerebral haemorrhage in rats.
Although intracerebral haemorrhage (ICH) entails the highest rates of mortality and disability of all stroke subtypes, efficient neuroprotective therapy is still needed. As functional recovery is a major endpoint in clinical trials, preclinical studies must demonstrate the potential of drugs to improve the sensorimotor and cognitive function of animals. In addition, behavioural studies should be performed on the long-term in order to truly mimic clinical needs. The aim of our study was to characterise a model of intracerebral haemorrhage using both histology and long-term behaviour. ICH was induced in rats by an intrastriatal injection of collagenase. Histology was performed 24h, 7 days and 2 months after ICH. Among a set of sensorimotor tests, we discriminate those able to reveal long-term deficits (up to 2 months) after cerebral haemorrhage. Our five behavioural tests (a neurological score, an adhesive removal test, two beam-walking tests and ipsilateral circling induced by dexamphetamine) proved to be effective in revealing sensorimotor deficits up to 35 days or more after cerebral haemorrhage. In conclusion, these behavioural tests appear of particular interest to screen protective agents that may exhibit benefits in patients who suffer ICH.
Beray-Berthat
Virginie
V
Equipe de Recherche "Pharmacologie de la Circulation Cérébrale" (EA4475), Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France. virginie.berthat@parisdescartes.fr
Delifer
Céline
C
Besson
Valérie C
VC
Girgis
Haymen
H
Coqueran
Bérard
B
Plotkine
Michel
M
Marchand-Leroux
Catherine
C
Margaill
Isabelle
I
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology; D002536:Cerebral Arteries / Q000187:drug effects* / Q000473:pathology* / Q000503:physiopathology; D002543:Cerebral Hemorrhage / Q000139:chemically induced* / Q000150:complications / Q000503:physiopathology; D017364:Collagenases / Q000633:toxicity*; D003913:Dextroamphetamine; D004185:Disability Evaluation; D004195:Disease Models, Animal; D007794:Lameness, Animal / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology; D008297:Male; D009460:Neurologic Examination; D051381:Rats; D017207:Rats, Sprague-Dawley; D013995:Time
D017364:Collagenases; D003913:Dextroamphetamine