title: Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila.
abstract: Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tsuda
authors.forename: Ryousuke
authors.initials: R
authors.lastname: Ootaka
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ohkura
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kishita
authors.forename: Ki-Hyeon
authors.initials: KH
authors.lastname: Seong
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics; D008136:Longevity / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010269:Paraquat / Q000633:toxicity; D010641:Phenotype; D050050:Protein Carbonylation / Q000187:drug effects; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D016877:Oxidants; C516042:Trx-2 protein, Drosophila; D013879:Thioredoxins; D010269:Paraquat