The antibacterial potency of the medicinal maggot, Lucilia sericata (Meigen): variation in laboratory evaluation.
Research to quantify the potency of larval excretion/secretion from Lucilia sericata using liquid culture assays has produced contradictory results. In this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and the effects of varying growing conditions in assays. Results demonstrate that factors such as number of larvae, species of bacteria and addition of nutrient influence its antibacterial potency. Therefore a standardised method should be employed for liquid culture assays when investigating the antibacterial activity of larval excretion/secretion from L. sericata.
Barnes
Katherine M
KM
School of Natural and Applied Sciences, University of Lincoln, Brayford Pool, Lincoln, LN6 7TS, UK. K.Barnes@shu.ac.uk
Dixon
Ronald A
RA
Gennard
Dorothy E
DE
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D019411:Clinical Laboratory Techniques / Q000592:standards*; D004175:Diptera / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D007814:Larva / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012107:Research Design
D000900:Anti-Bacterial Agents