Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil.
A metabolic network was constructed for the Acremonium chrysogenum cultivation fed with soybean oil. Metabolic flux analysis indicated that the shift from exponential growth to rapid cephalosporin C (CPC) formation was accompanied by 1.63- and 5-fold carbon flux enlargement in TCA cycle and glyoxylate by-pass, respectively. The flux via pentose phosphate pathway branch was little affected during the rapid CPC formation period; the contributory explanation was that 35.6% of NADPH was consumed in the dissimilation of fatty acids. Estimation of NADPH, ATP generation, and consumption demonstrated that, with soybean oil as carbon source in rapid CPC formation phase, the NADPH consumed in fatty acid catabolism was fourfold greater than that used in the CPC biosynthesis-relevant part; simultaneously, more than 90% energy spent was not directly related to the CPC formation. Therefore, the improvement of CPC production yield through optimization of the NADPH, ATP generation, and consumption was put forward.
Li
Jianhua
J
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, People's Republic of China.
Yang
Yiming
Y
Chu
Ju
J
Huang
Mingzhi
M
Li
Liang
L
Zhang
Xiaochun
X
Wang
Yonghong
Y
Zhuang
Yingping
Y
Zhang
Siliang
S
D000164:Acremonium / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000737:chemistry*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000737:chemistry; D002511:Cephalosporins / Q000494:pharmacology; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods; D009249:NADP / Q000737:chemistry*; D010427:Pentose Phosphate Pathway; D013024:Soybean Oil / Q000378:metabolism*; D013997:Time Factors
D002511:Cephalosporins; C025163:cephalosporin C; D009249:NADP; D002244:Carbon; D013024:Soybean Oil; D000255:Adenosine Triphosphate; D005947:Glucose