Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland.
This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood, resident ((RES)PMN) and inflammatory ((INF)PMN) polymorphonuclear leukocytes (PMN) from heifer mammary glands. The fresh population of (RES)PMN contained a statistically significant higher proportion of CD14+, apoptotic and necrotic cells than did populations of (INF)PMN and blood PMN. In vitro cultivation of (RES)PMN, (INF)PMN and blood PMN led to concurrent increase of apoptotic, necrotic and CD14+ cells. A positive correlation was found between the proportions of both apoptotic and necrotic PMN and CD14+ PMN as determined by three-color flow cytometry analysis. Our study confirmed that expression of CD14 in blood PMN, (RES)PMN and (INF)PMN from heifer mammary glands was accompanied by apoptosis and necrosis.
Sladek
Zbysek
Z
Department of Morphology, Physiology and Veterinary Sciences, Mendel University of Agriculture and Forestry, Zemedelska 1, Brno 613 00, Czech Republic. zbysek.sladek@mendelu.cz
Rysanek
Dusan
D
D000818:Animals; D002417:Cattle; D016923:Cell Death / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000502:physiology*; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*
D018950:Lipopolysaccharide Receptors