title: Pharmacokinetic properties of paeoniflorin, albiflorin and oxypaeoniflorin after oral gavage of extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba in rats.
abstract: OBJECTIVE
  To establish a HPLC-MS method and investigate the pharmacokinetic properties of paeoniflorin, albiflorin and oxypaeoniflorin and the pharmacokinetics difference of Radix Paeoniae Rubra and Radix Paeoniae Alba.
  
  
  METHODS
  The extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba were separately administrated to rats. The concentrations of paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma were determined by HPLC-ESI-MS method. The plasma samples were pretreated by protein precipitation with methanol and chromatographic separation was performed on a C(18) column with a mobile phase consisted of 0.1% formic acid and methanol (67:33, v/v). The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization (ESI) source operating in the negative ionization mode. Main pharmacokinetic parameters were estimated and the total AUC of the three components were compared.
  
  
  RESULTS
  The pharmacokinetic parameters of paeoniflorin, albiflorin and oxypaeoniflorin were significantly different. There was significant difference between the pharmacokinetic characteristics of Radix Paeoniae Rubra and Radix Paeoniae Alba.
  
  
  CONCLUSIONS
  A specific and sensitive HPLC-ESI-MS method was developed for simultaneous determination of paeoniflorin, albiflorin and oxypaeoniflorin in rat plasma and was successfully applied to pharmacokinetic study. The results might be helpful for the investigation of different effects of Radix Paeoniae Rubra and Radix Paeoniae Alba.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.forename: Man
authors.initials: M
authors.lastname: Liu
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Dezhi
authors.initials: D
authors.lastname: Kong
authors.forename: Kunfeng
authors.initials: K
authors.lastname: Duan
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001565:Benzoates / Q000097:blood / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D039821:Monoterpenes; D029596:Paeonia; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C047593:oxypaeoniflora