Image processing guided analysis for estimation of bacteria colonies number by means of optical transforms.
A novel method for evaluation of bacterial colonies number (Colony Forming Units--CFU), is described. Proposed algorithm, based on the Mellin transform, allows the CFU evaluation, invariant for the spatial orientation and scale changes. The proposed method involves image recording of bacteria grown in Petri dishes, calculation of the Fourier spectrum followed by coordinates transformation, and determination of the Mellin transform. It was proved that there is a high correlation between CFU and maxima of Mellin spectra. The method was practically implemented for evaluation of antibacterial activity of silver-based nanomaterials and the effect of an additional laser light irradiation.
Buzalewicz
Igor
I
Bio-Optics Group, Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, WybrzeSe Wyspiańskiego 27, 50-370 Wroclaw, Poland. igor.buzalewicz@pwr.wroc.pl
Wysocka-Król
Katarzyna
K
Podbielska
Halina
H
D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial / Q000379:methods*; D003198:Computer Simulation; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D055620:Optical Phenomena; D012834:Silver / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D012834:Silver