title: Direct and rapid electrochemical immunosensing system based on a conducting polymer.
abstract: A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen-antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.
authors.affiliation: Laboratoire Interfaces-Traitements-Organisation et Dynamique des Systèmes (ITODYS), associé au CNRS-UMR 7086, Université Paris Diderot-Paris 7, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Piro
authors.forename: Q D
authors.initials: QD
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Reisberg
authors.forename: V
authors.initials: V
authors.lastname: Noel
authors.forename: L A
authors.initials: LA
authors.lastname: Dang
authors.forename: H T
authors.initials: HT
authors.lastname: Duc
authors.forename: M C
authors.initials: MC
authors.lastname: Pham
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D009285:Naphthoquinones / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D044967:Serum / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D009285:Naphthoquinones; D011108:Polymers; D013438:Sulfhydryl Compounds; D019342:Acetic Acid; C035342:1,4-naphthoquinone