Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum.
A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 degrees C) and in the presence of 0-1% (w/v) NaCl. It contained MK-7 as the predominant menaquinone. The isolate had activities of catalase, oxidase and beta-galactosidase and hydrolyzed aesculin, casein, carboxymethyl-cellulose, starch and L-tyrosine. The major cellular fatty acids were summed feature 3 (C(16:1)omega7c and/or iso-C(15:0) 2OH) and iso-C(15:0). The DNA G+C content was 42.6 mol%. This isolate belonged to the genus Mucilaginibacter based on phylogenetic analysis using 16S rRNA gene sequences. The nearest phylogenetic neighbors of strain DR-f4(T) were Mucilaginibacter lappiensis ANJL12(T) and Mucilaginibacter rigui WPCB133(T), with 16S rRNA gene sequence similarity levels of 96.9% and 96.4%, respectively. The genotypic and phenotypic evidence suggests that strain DR-f4(T) should be classified as a novel species, for which the name Mucilaginibacter dorajii sp. nov. is proposed. The type strain for the novel species is DR-f4(T) (=KACC 14556(T)=JCM 16601(T)).
Kim
Byung-Chun
BC
Biological Resources Center, KRIBB, Daejeon, Korea.
Lee
Kang Hyun
KH
Kim
Mi Na
MN
Lee
Jongtae
J
Shin
Kee-Sun
KS
D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D031238:Platycodon / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S