Hoechst-IR: an imaging agent that detects necrotic tissue in vivo by binding extracellular DNA.
Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.
Dasari
Madhuri
M
The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Lee
Sungmun
S
Sy
Jay
J
Kim
Dongin
D
Lee
Seungjun
S
Brown
Milton
M
Davis
Michael
M
Murthy
Niren
N
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003952:Diagnostic Imaging; D005688:Galactosamine / Q000494:pharmacology; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology*; D009690:Nucleic Acid Conformation
D007211:Indoles; D008070:Lipopolysaccharides; D005688:Galactosamine; D004247:DNA