Layer I as a putative neurogenic niche in young adult guinea pig cerebrum.
A considerable number of cells expressing typical immature neuronal markers including doublecortin (DCX+) are present around layer II in the cerebral cortex of young and adult guinea pigs and other larger mammals, and their origin and biological implication await further characterization. We show here in young adult guinea pigs that these DCX+ cells are accompanied by in situ cell division around the superficial cortical layers mostly in layer I, but they co-express proliferating cell nuclear antigen (PCNA) and an early neuronal fate determining factor, PAX6. A small number of these DCX+ cells also colocalize with BrdU following administration of this mitotic indicator. Cranial X-ray irradiation causes a decline of DCX+ cells around layer II, and novel environmental exploration induces c-Fos expression among these cells in several neocortical areas. Together, these data are compatible with a notion that DCX+ cortical neurons around layer II might derive from proliferable neuronal precursors around layer I in young adult guinea pig cerebrum, and that these cells might be modulated by experience under physiological conditions.
Xiong
Kun
K
Department of Anatomy and Neurobiology, Central South University Xiangya Medical School, Changsha, Hunan, China.
Cai
Yan
Y
Zhang
Xue-Mei
XM
Huang
Ju-Fang
JF
Liu
Zhong-Yu
ZY
Fu
Guang-Ming
GM
Feng
Jia-Chun
JC
Clough
Richard W
RW
Patrylo
Peter R
PR
Luo
Xue-Gang
XG
Hu
Chun-Hong
CH
Yan
Xiao-Xin
XX
D000818:Animals; D002455:Cell Division; D054022:Cerebrum / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D000089622:Doublecortin Domain Proteins; D005136:Eye Proteins / Q000032:analysis; D006168:Guinea Pigs; D018398:Homeodomain Proteins / Q000032:analysis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D019579:Neocortex / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D009419:Nerve Tissue Proteins / Q000032:analysis; D055495:Neurogenesis; D009479:Neuropeptides / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D012097:Repressor Proteins / Q000032:analysis
D000089622:Doublecortin Domain Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D018809:Proliferating Cell Nuclear Antigen; D016760:Proto-Oncogene Proteins c-fos; D012097:Repressor Proteins