Reciprocal communication between endometrial stromal cells and macrophages.
This study tested the hypothesis that reciprocal communication occurs between macrophages and cultured human endometrial stromal cells and that this communication may contribute to the pathology of endometriosis. An endometrial stromal cell line (telomerase-immortalized human endometrial stromal cell [T-HESC]) was treated with macrophage-conditioned medium (CM) +/- estradiol + progesterone. Macrophages were treated without or with T-HESC CM. DNA microarray identified 716 differentially expressed genes in T-HESCs in response to factors secreted by macrophages. Upregulated genes in T-HESC included interleukin 8 (IL-8)/chemokine (C-X-C motif) ligand 8 (CXCL8), matrix metalloproteinase 3 (MMP3), phospholamban, cysteine-rich angiogenic inducer 61 (CYR61), connective tissue growth factor (CTGF), tenascin C, and nicotinamide N-methyltransferase (NNMT), whereas integrin alpha-6 was downregulated. In contrast, 15 named genes were differentially expressed in macrophages in response to factors secreted by endometrial stromal cells. The data document reciprocal communication between macrophages and endometrial stromal cells and suggest that interaction with macrophages stimulates the expression of genes in endometrial stromal cells that may support the establishment of endometriosis.
Eyster
Kathleen M
KM
Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, SD 57069, USA. Kathleen.Eyster@usd.edu
Hansen
Keith A
KA
Winterton
Emily
E
Klinkova
Olga
O
Drappeau
Donis
D
Mark-Kappeler
Connie J
CJ
D017077:Culture Media, Conditioned / Q000378:metabolism; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D019899:Paracrine Communication / Q000187:drug effects / Q000235:genetics; D011388:Prolactin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020298:U937 Cells
D017077:Culture Media, Conditioned; D004958:Estradiol; D011388:Prolactin; D017258:Medroxyprogesterone Acetate