Anti-obesity effect of carboxymethyl chitin by AMPK and aquaporin-7 pathways in 3T3-L1 adipocytes.
The aim of this study was to investigate the anti-obesity effect of carboxymethyl-chitin (CM-chitin), a water-soluble derivative of chitin, by measuring lipid accumulation and adipogenesis related factors in 3T3-L1 adipocytes. CM-chitin was synthesized by means of carboxymethylation reaction. Its inhibitory effect on lipid accumulation was investigated by measuring triglyceride content and glycerol release level. The gene and protein levels associated with adipogenesis were determined using reverse transcriptase-polymerase chain reaction and Western blot analysis. Treatment with CM-chitin reduced triglyceride content and enhanced glycerol secretion in a dose-dependent manner. CM-chitin induced the down-regulation of adipogenesis related transcriptional factors and adipocyte specific gene promoters. Moreover, the specific mechanism by CM-chitin was confirmed by transcriptional activations of the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and aquaporin-7. These results suggest that CM-chitin exerts anti-adipogenic effect on lipid accumulation through modulations of AMPK and aquaporin-7 signal pathways.
Kong
Chang-Suk
CS
Department of Food and Nutrition, College of MEdical and Life Science, Silla University, Busan 617-736, Republic of Korea.
Kim
Jung-Ae
JA
Bak
Soon-Sun
SS
Byun
Hee-Guk
HG
Kim
Se-Kwon
SK
D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D020346:Aquaporins / Q000378:metabolism*; D002454:Cell Differentiation; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation; D050356:Lipid Metabolism; D051379:Mice; D009765:Obesity / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014280:Triglycerides / Q000032:analysis
D019440:Anti-Obesity Agents; C491927:Aqp7 protein, mouse; D020346:Aquaporins; D014280:Triglycerides; D002686:Chitin; C044872:O-(carboxymethyl)chitin; D055372:AMP-Activated Protein Kinases