title: [Comparative study on the analgesic effect of electroacupuncture at "Sanyinjiao" (SP 6), "Hegu" (LI 4) and "Xuehai" (SP 10) in dysmenorrhea rats].
abstract: OBJECTIVE
  To observe and compare the analgesic effect of electroacupuncture (EA) at "Sanyinjiso" (SP 6), "Hegu" (LI 4) and "Xuehai" (SP 10) in dysmenorrhea rats so as to investigate the functional specificity of acupoint.
  
  
  METHODS
  One hundred SD female rats aged three-month-old in the diestrus examined by the vaginal smear screening were randomly divided into a saline group, a model group, a Sanyinjiao (SF 6) group, a Hegu (LI 4) group and a Xuehai (SF 10) group, 20 rats in each group. The rats in the last four groups were given Estradiol Benzoate combined with Oxytocin to prepare the models of dysmenorrhea and in the saline group were given the same dose of saline. Last three groups were treated with electroacupuncture (EA) at Sanyinjiao" (SF 6), "Hegu" (LI 4) and 'Xuehai" (SF 10) respectively. The writhing behaviors of the rats in each group were observed within 20 min performing electroacupuncture from the start of the pain and the waveforms of uterine contraction were recorded.
  
  
  RESULTS
  In terms of the writhing behaviors, the writhing latency in the Hegu (LI 4) group was extended significantly (P < 0.05, P < 0.01) and the writhing numbers in the Hegu (LI 4) group were decreased significantly (both P < 0.05) as compared with those in the Sanyinjiao (SF 6) group and in the Xuehai (SF 10) group. In terms of the waveforms of uterine contraction, compared with that in the model group, the amplitude and the activity of uterine contraction in both Sanyinjiao (SF 6) group and Hegu (LI 4) group were decreased significantly (all P < 0.05), and with no significant difference between the Xuehai (SF 10) and the model group (P > 0.05).
  
  
  CONCLUSIONS
  For comprehensive assessment of the analgesic effect of electroacupuncture at different acupoints by using the writhing behaviors and the waveforms of uterine contraction, "Hegu" (LI 4) is the best, "Sanyinjiao" (SP 6) is the next and "Xuehai" (SP 10) is the third. showing a relatively functional specificity of the acupoint.
authors.affiliation: College of Acupuncture and Moxibustion, Beijing University of CM, Beijing 100029, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xu
authors.forename: Lu-Fen
authors.initials: LF
authors.lastname: Zhang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Song
authors.forename: Xiao-Xuan
authors.initials: XX
authors.lastname: Ren
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Zhao
authors.forename: Meng-Wei
authors.initials: MW
authors.lastname: Guo
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Sun
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
mesh_terms: D015667:Acupuncture Analgesia; D015669:Acupuncture Points; D000818:Animals; D004195:Disease Models, Animal; D004412:Dysmenorrhea / Q000628:therapy*; D015671:Electroacupuncture; D005260:Female; D006801:Humans; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley