Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae).
Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1(T) and FFA3(T), and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1(T)) and 99.35 % (FFA3(T)) to Ignatzschineria larvae L1/68(T). The level of gene sequence similarity between strains FFA1(T) and FFA3(T) was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1(T) and FFA3(T) shared 24 % DNA-DNA relatedness. DNA-DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1(T) and 44 % for strain FFA3(T)) and I. larvae L1/68(T) genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1(T) and FFA3(T), respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C(16 : 0), summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6c), CyC(19 : 0)ω8c and C(14 : 0). The results of DNA-DNA hybridization between the two new strains and I. larvae L1/68(T), in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1(T) and FFA3(T) represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1(T) = DSM 22309(T) = KCTC 22643(T) = NCIM 5325(T)) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3(T) = DSM 22310(T) = KCTC 22644(T) = NCIM 5326(T)) are proposed.
Gupta
Arvind Kumar
AK
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
Dharne
Mahesh Shantappa
MS
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
Rangrez
Ashraf Yusuf
AY
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
Verma
Pankaj
P
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
Ghate
Hemant V
HV
Department of Zoology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune 411004, Maharashtra, India.
Rohde
Manfred
M
Helmholtz-Zentrum für Infektionsforschung GmbH, D-38124 Braunschweig, Germany.
Patole
Milind Shivaji
MS
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
Shouche
Yogesh Shreepad
YS
Microbial Culture Collection, Hindustan Antibiotics Ltd. Complex, Pimpri, Pune 411018, Maharashtra, India.|Molecular Biology Unit, National Centre for Cell Science, Pune 411007, Maharashtra, India.
D000332:Aerobiosis; D000818:Animals; D001482:Base Composition; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058493:Sarcophagidae / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase