title: Solvent inhalation (toluene and n-hexane) during the brain growth spurt impairs the maturation of frontal, parietal and occipital cerebrocortical neurons in rats.
abstract: Solvent abuse during pregnancy may cause "fetal solvent syndrome", which is characterized by mild brain atrophy and associated with behavioral, cognitive, and emotional abnormalities. The present study investigated whether solvent inhalation during the preweaning period (P2-P21) alters the morphological maturation of frontal, parietal, and occipital cortical neurons. Twelve hours after delivery (postnatal day 0, P0), litters were cross-fostered, culled to 8 pups/dam and housed together with a dam in standard laboratory cages. Litters were randomly assigned to the "air-only" group (n=64, 8 litters) and to the "solvent-sniffer" group (n=72, 9 litters). During P2-P21, each animal was exposed daily to either organic solvent vapors (75% toluene and 18% n-hexane, a solvent mixture commonly found in glues and adhesives) or clean air. To determine the impact of early solvent inhalation on cortical neuronal differentiation, brains were stained using the Golgi-Cox-Sholl procedure to quantitatively assess neocortical pyramidal cell dendrogenesis. Preweaning, solvent-exposed animals displayed dramatic impairments in dendritic growth as well as significant reductions in brain weight and size.
authors.affiliation: Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile. rodrigo.pascual@ucv.cl
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Pascual
authors.forename: Luz
authors.initials: L
authors.lastname: Aedo
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Meneses
authors.forename: Daniela
authors.initials: D
authors.lastname: Vergara
authors.forename: Alvaro
authors.initials: A
authors.lastname: Reyes
authors.forename: Carlos
authors.initials: C
authors.lastname: Bustamante
mesh_terms: D000280:Administration, Inhalation; D000375:Aging / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000254:growth & development* / Q000473:pathology*; D004338:Drug Combinations; D005260:Female; D006586:Hexanes / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D009474:Neurons / Q000473:pathology* / Q000502:physiology*; D051381:Rats; D014050:Toluene / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D004338:Drug Combinations; D006586:Hexanes; C026385:n-hexane; D014050:Toluene