In vivo investigation of human cone photoreceptors with SLO/OCT in combination with 3D motion correction on a cellular level.
We present a further improvement of our SLO/OCT imaging system which enables to practically eliminate all eye motion artifacts with a correction accuracy approaching sub-cellular dimensions. Axial eye tracking is achieved by using a hardware based, high speed tracking system that consists of a rapid scanning optical delay line in the reference arm of the interferometer. A software based algorithm is employed to correct for transverse eye motion in a post-processing step. The instrument operates at a frame rate of 40 en-face fps with a field of view of approximately 1 degrees x 1 degrees. Dynamic focusing enables the recording of 3D volumes of the human retina with cellular resolution throughout the entire imaging depth. Several volumes are stitched together to increase the total field of view. Different features of the three dimensional structure of cone photoreceptors are investigated in detail and at different eccentricities from the fovea.
Pircher
Michael
M
Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringerstr. 13, Vienna, Austria. michael.pircher@meduniwien.ac.at
Götzinger
Erich
E
Sattmann
Harald
H
Leitgeb
Rainer A
RA
Hitzenberger
Christoph K
CK
D000328:Adult; D016477:Artifacts; D004867:Equipment Design; D005133:Eye Movements; D005584:Fovea Centralis / Q000166:cytology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D017949:Retinal Cone Photoreceptor Cells / Q000166:cytology*; D055213:Retinal Pigment Epithelium / Q000166:cytology; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods*; D055815:Young Adult