Butyric acid stimulates bone sialoprotein gene transcription.
Butyric acid (sodium butyrate; BA) is an extracellular metabolite secreted from periodontopathic bacteria present in subgingival plaque. BA induces apoptosis of T and B cells, and acts as a potent inhibitor of histone deacetylases. Bone sialoprotein (BSP) is thought to function in the initial mineralization of bone, and may be crucial for osteoblast differentiation, bone matrix mineralization and tumor metastasis. In the present study we investigated the regulation of BSP transcription by BA in rat osteoblast-like ROS17/2.8 cells. At 12 h, BA (10(-4) M) increased the level of BSP mRNA, and enhanced the luciferase activity of the construct pLUC3, which includes the promoter sequence between nucleotides -116 and +60. Transcriptional stimulation by BA was abrogated in the pLUC3 construct which containing a 2-bp mutation in the fibroblast growth factor 2 response element (FRE). Gel shift analyses showed that BA increased the binding of nuclear protein to FRE. These data suggest that BA increases the transcription of the BSP gene mediated through FRE in the rat BSP gene promoter, and induces osteoblast activity in the early stage of bone formation.
Yang
Li
L
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Li
Zhengyang
Z
Li
Xinyue
X
Wang
Zhitao
Z
Wang
Shuang
S
Sasaki
Yoko
Y
Takai
Hideki
H
Ogata
Yorimasa
Y
D000818:Animals; D020029:Base Pairing / Q000235:genetics; D015152:Blotting, Northern; D020148:Butyric Acid / Q000494:pharmacology*; D023081:CCAAT-Binding Factor / Q000187:drug effects; D050882:CREB-Binding Protein / Q000187:drug effects; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000187:drug effects / Q000235:genetics; D018398:Homeodomain Proteins / Q000187:drug effects; D058574:Integrin-Binding Sialoprotein; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D051381:Rats; D020218:Response Elements / Q000187:drug effects / Q000235:genetics; D012795:Sialoglycoproteins / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors; D050817:Transcription Factor Pit-1 / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
D023081:CCAAT-Binding Factor; D018398:Homeodomain Proteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D050817:Transcription Factor Pit-1; D016222:Fibroblast Growth Factor 2; D020148:Butyric Acid; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat