TP53 gene mutations in prostate cancer progression.
BACKGROUND
We assessed the predictive value of TP53 mutations and prostate-specific antigen (PSA) for tumor progression in prostate cancer (PCa) patients.


METHODS
Ninety tumor tissue samples of patients with PCa from radical prostatectomy were used. Tumor progression was estimated biochemically by the PSA level (> 0.2 microg/l) or by detection of metastases. Screening for TP53 mutations was performed by temperature gradient gel electrophoresis (TGGE) in exon-specific manner. Follow-up data were collected from medical protocols. Statistical analysis was performed by uni- and multivariate techniques.


RESULTS
In 32 out of 90 patients (35.6%), TP53 mutations were detected. Thirteen out of 32 patients (40.6%) with TP53 mutations and nine out of 58 patients (15.5%) with TP53 wild-type showed tumor progression after 25 and 45 months, respectively.


CONCLUSIONS
TP53 mutations in exon 7 and exon 8 are factors of tumor progression in PCa. Their contribution to tumor recurrence is more significant than tumor stage and pretherapeutic PSA level.
Ecke
Thorsten H
TH
HELIOS Hospital, Department of Urology, Bad Saarow, Germany. thorsten.ecke@helios-kliniken.de
Schlechte
Horst H
HH
Schiemenz
Katrin
K
Sachs
Markus D
MD
Lenk
Severin V
SV
Rudolph
Birgit D
BD
Loening
Stefan A
SA
D004252:DNA Mutational Analysis; D018450:Disease Progression; D005091:Exons; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009362:Neoplasm Metastasis; D017430:Prostate-Specific Antigen / Q000737:chemistry; D011468:Prostatectomy / Q000379:methods; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D012307:Risk Factors; D013696:Temperature
D017430:Prostate-Specific Antigen