Relationship between lipid peroxidation and antioxidant status in the muscle of German Holstein bulls Fed n-3 and n-6 PUFA-enriched diets.
This study evaluated the influence of different n-3 and n-6 PUFA-enriched diets on the relationship between lipid peroxidation and antioxidant status by analyzing fatty acids (FA), lipid peroxidation, antioxidant capacity (AOC), antioxidant enzymes, trace elements, and fat-soluble vitamins in the longissimus muscle. Diet caused significant changes in muscle FA composition, leading to accumulation of beneficial n-3 FA. beta-Carotene and catalase activity were significantly elevated in muscle of the n-3 PUFA-enriched diet group compared to the n-6 PUFA-enriched diet group. Lipid peroxidation was higher in muscle of the n-3 PUFA-enriched diet group after 15 min of reaction time. There was no significant effect of diet on AOC, but it increased with reaction time. The present results suggest that the antioxidant defense in muscle of the n-3 PUFA-enriched diet group could balance reactive substances under low oxidative conditions. However, the antioxidant capacity was not sufficient under abundant accumulation of reactive substances.
Mahecha
Liliana
L
Grupo Grica, Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellin, Colombia.
Dannenberger
Dirk
D
Nuernberg
Karin
K
Nuernberg
Gerd
G
Hagemann
Elke
E
Martin
Jörg
J
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D011897:Random Allocation
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated