title: Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-beta-lactamases.
abstract: Acquired metallo-beta-lactamase (MBL) production is an important mechanism of carbapenem resistance. To our knowledge, carriage of two different MBLs has not been described previously in Acinetobacter spp. We present the characteristics of five Acinetobacter isolates carrying two different MBL genes. The species of all five Acinetobacter isolates with two different MBL genes were Acinetobacter genomospecies 10, and bla(IMP-1), bla(VIM-2) and bla(SIM-1) may coexist in this species. Minimum inhibitory concentrations (MICs) of imipenem for all five isolates with two different MBLs were >or=32 microg/mL, whilst those for two segregants that lost both MBLs were 0.5 microg/mL. The presence of MBL gene-carrying integrons with identical structures suggested the easily transferable nature of the elements, whilst instability of the MBL genes indicated potentially erroneous phenotypic and genetic characterisation.
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.forename: Chang Ki
authors.initials: CK
authors.lastname: Kim
authors.forename: Seong Geun
authors.initials: SG
authors.lastname: Hong
authors.forename: Jonghyeon
authors.initials: J
authors.lastname: Choi
authors.forename: Sungwook
authors.initials: S
authors.lastname: Song
authors.forename: Eunmi
authors.initials: E
authors.lastname: Koh
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Jong Hwa
authors.initials: JH
authors.lastname: Yum
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Docquier
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology*; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases