A new allergen from ragweed (Ambrosia artemisiifolia) with homology to art v 1 from mugwort.
Art v 1, the major pollen allergen of the composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with a bulky arabinogalactan-protein moiety. A close relative of mugwort, ragweed (Ambrosia artemisiifolia) is an important allergen source in North America, and, since 1990, ragweed has become a growing health concern in Europe as well. Weed pollen-sensitized patients demonstrated IgE reactivity to a ragweed pollen protein of apparently 29-31 kDa. This reaction could be inhibited by the mugwort allergen Art v 1. The purified ragweed pollen protein consisted of a 57-amino acid-long defensin-like domain with high homology to Art v 1 and a C-terminal proline-rich domain. This part contained hydroxyproline-linked arabinogalactan chains with one galactose and 5 to 20 and more alpha-arabinofuranosyl residues with some beta-arabinoses in terminal positions as revealed by high field NMR. The ragweed protein contained only small amounts of the single hydroxyproline-linked beta-arabinosyl residues, which form an important IgE binding determinant in Art v 1. cDNA clones for this protein were obtained from ragweed flowers. Immunological characterization revealed that the recombinant ragweed protein reacted with >30% of the weed pollen allergic patients. Therefore, this protein from ragweed pollen constitutes a novel important ragweed allergen and has been designated Amb a 4.
Léonard
Renaud
R
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), 1190 Vienna, Austria.
Wopfner
Nicole
N
Department of Molecular Biology, Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, 5020 Salzburg, Austria.
Pabst
Martin
M
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), 1190 Vienna, Austria.
Stadlmann
Johannes
J
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), 1190 Vienna, Austria.
Petersen
Bent O
BO
Carlsberg Laboratory, 2500 Valby, Denmark.
Duus
Jens Ø
JØ
Carlsberg Laboratory, 2500 Valby, Denmark.
Himly
Martin
M
Department of Molecular Biology, Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, 5020 Salzburg, Austria.
Radauer
Christian
C
Department of Pathophysiology, Medical University of Vienna, 1090 Vienna, Austria.
Gadermaier
Gabriele
G
Department of Molecular Biology, Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, 5020 Salzburg, Austria.
Razzazi-Fazeli
Ebrahim
E
VetOMICS, Core Facility for Research, University of Veterinary Medicine, 1220 Vienna, Austria.
Ferreira
Fatima
F
Department of Molecular Biology, Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, 5020 Salzburg, Austria.
Altmann
Friedrich
F
Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), 1190 Vienna, Austria. Electronic address: friedrich.altmann@boku.ac.at.
D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D031164:Ambrosia / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D052179:Antigens, Plant; D019306:Artemisia / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D005060:Europe / Q000453:epidemiology; D005685:Galactans / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D009656:North America / Q000453:epidemiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D017434:Protein Structure, Tertiary; D006255:Rhinitis, Allergic, Seasonal / Q000453:epidemiology / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
D000485:Allergens; C552169:Amb a 4 protein, ragweed; D052179:Antigens, Plant; C102724:Art v 1 protein, mugwort; D018076:DNA, Complementary; D005685:Galactans; D010940:Plant Proteins; D007073:Immunoglobulin E; C005653:arabinogalactan