title: Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.
abstract: In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.
authors.affiliation: Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8602, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Ryoko
authors.initials: R
authors.lastname: Morita
authors.forename: Takamasa
authors.initials: T
authors.lastname: Mizoguchi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Matsuo
authors.forename: Miho
authors.initials: M
authors.lastname: Isoda
authors.forename: Tohru
authors.initials: T
authors.lastname: Ishitani
authors.forename: Ajay B
authors.initials: AB
authors.lastname: Chitnis
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: J Gage
authors.initials: JG
authors.lastname: Crump
authors.forename: Katsuto
authors.initials: K
authors.lastname: Hozumi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yonemura
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawakami
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Itoh
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019521:Body Patterning; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D019070:Cell Lineage; D002522:Chlorocebus aethiops; D004705:Endocytosis; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D009672:Notochord / Q000502:physiology*; D051880:Receptors, Notch / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D025801:Ubiquitin; D029961:Zebrafish Proteins; C000606276:jag1a protein, zebrafish; D044767:Ubiquitin-Protein Ligases; C471932:mib1 protein, zebrafish