title: Hoechst-IR: an imaging agent that detects necrotic tissue in vivo by binding extracellular DNA.
abstract: Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.
authors.affiliation: The Wallace H. Coulter Department of Biomedical Engineering and Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Dasari
authors.forename: Sungmun
authors.initials: S
authors.lastname: Lee
authors.forename: Jay
authors.initials: J
authors.lastname: Sy
authors.forename: Dongin
authors.initials: D
authors.lastname: Kim
authors.forename: Seungjun
authors.initials: S
authors.lastname: Lee
authors.forename: Milton
authors.initials: M
authors.lastname: Brown
authors.forename: Michael
authors.initials: M
authors.lastname: Davis
authors.forename: Niren
authors.initials: N
authors.lastname: Murthy
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003952:Diagnostic Imaging; D005688:Galactosamine / Q000494:pharmacology; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology*; D009690:Nucleic Acid Conformation
chemical_list: D007211:Indoles; D008070:Lipopolysaccharides; D005688:Galactosamine; D004247:DNA