Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR.
Laboratory bred female Aedes aegypti (L.) was used to determine sensitivity of multiplex PCR for detecting human blood meal. Human blood DNA was detected in live fully fed mosquitoes until 3 days after blood feeding, and for 4 weeks when stored at -20 degrees C. Among 890 field caught female mosquito samples examined for vertebrate DNA by multiplex PCR, results were positive for human, pig, dog, cow and mixture of 2 host DNA at 86.1, 3.4, 2.1, 1.0 and 3.6%, respectively, while 3.9% of the samples were negative. Blood feeding pattern must be considered when mosquito control strategies become employed.
Siriyasatien
Padet
P
Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. padet.s@chula.ac.th
Pengsakul
Theerakamol
T
Kittichai
Veerayuth
V
Phumee
Atchara
A
Kaewsaitiam
Sakchai
S
Thavara
Usavadee
U
Tawatsin
Apiwat
A
Asavadachanukorn
Preecha
P
Mulla
Mir S
MS
D000330:Aedes / Q000502:physiology*; D000818:Animals; D001800:Blood Specimen Collection; D002417:Cattle; D004247:DNA / Q000097:blood*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007303:Insect Vectors; D016133:Polymerase Chain Reaction; D013552:Swine
D004247:DNA