[Evaluation on running status of Chinese Polio Laboratories Network in 2008].
OBJECTIVE
In order to evaluate the running status and provide the laboratory data for maintaining polio-free status in China, the virology surveillance database of Chinese Polio Laboratories Network (not include Hong Kong, Macao, and Taiwan)in 2008 were analyzed.


METHODS
The case investigation data of Acute Flaccid Paralysis(AFP)cases reported by 31 provinces (municipal, autonomous regions) through EPI surveillance information management system and the database of National Polio Laboratory (NPL) were analyzed, and the indicators of running status of Chinese Polio Laboratories Network were evaluated.


RESULTS
10,116 stool samples were collected from 5116 AFP cases by Chinese Polio Laboratories Network in 2008, and viral isolation and identification of all stool samples were done according to 4th World Health Organization (WHO) Polio Laboratory Manual. The rate of viral isolation and identification performed within 28d was 94.9%. 189 polioviruses (PV) and 597 of non-polio enteroviruses (NPEV) were isolated from AFP cases, the isolatien rates were 3.72% and 11.74% respectively. 251 polio positive isolates were sent to NPL from 31 provincial polio laboratories. There were 318 single serotype PVs were performed VPI sequencing. And no wild polioviruses and Vaccine-derived Polioviruses (VDPVs) were found in 2008. NPL passed the proficiency test and got full accreditation for on-site review by WHO experts in 2008. All 31 provincial Polio laboratories passed the proficiency test with the same panel as NPL, and 13 provincial Polio laboratories joined and passed the on-site review by WHO experts.


CONCLUSIONS
The running status of Chinese Polio Laboratories Network was good, polio-free status was maintained in China in 2008. The Chinese polio laboratories network running is normaly, the laboratory surveillance system was sensitive and laboratory data were provided for maintaining the polio-free status in China.
Zhu
Shuang-li
SL
World Health Organization Western Pacific Region Regional Polio Laboratory, State Key Laboratory for Molecular Virology & Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Yan
Dong-mei
DM
Zhu
Hui
H
D036022:Capsid Proteins / Q000235:genetics; D016208:Databases, Factual; D006801:Humans; D007753:Laboratories / Q000592:standards / Q000706:statistics & numerical data*; D016366:Open Reading Frames / Q000235:genetics; D010264:Paraplegia / Q000453:epidemiology / Q000821:virology; D011051:Poliomyelitis / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D023321:Poliovirus Vaccines; D015397:Program Evaluation; D011786:Quality Control; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; D023321:Poliovirus Vaccines; C442540:VP1 protein, Poliovirus