Novel candidate substrates of AMP-activated protein kinase identified in red blood cell lysates.
AMPK is a metabolic stress-sensing kinase with important functions for red blood cell (RBC) survival. By using a proteomic approach, we identified putative AMPK targets in hemoglobin-depleted lysates of RBC, including metabolic enzymes, cytoskeletal proteins and enzymes involved in the oxidative stress response. These data tie in with the phenotypic observations of AMPKalpha1-deficient RBC and provide reference for future studies.
Thali
Ramon F
RF
Institute of Cell Biology, ETH Zurich, Zurich, Switzerland. ramon.thali@cell.biol.ethz.ch
Tuerk
Roland D
RD
Scholz
Roland
R
Yoho-Auchli
Yolanda
Y
Brunisholz
Rene A
RA
Neumann
Dietbert
D
D055372:AMP-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002846:Chromatography, Affinity; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology*; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D009532:Nickel / Q000737:chemistry; D013379:Substrate Specificity
D006454:Hemoglobins; D009532:Nickel; D055372:AMP-Activated Protein Kinases