title: Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry.
abstract: Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety.
authors.affiliation: Fisheries and Oceans Canada, Science Branch, Special Projects, P.O. Box 5667, St. John's, Newfoundland, Canada A1C 5X1. joe.banoub@dfo-mpo.gc.ca
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Banoub
authors.forename: Anas
authors.initials: A
authors.lastname: El Aneed
authors.forename: Alejandro M
authors.initials: AM
authors.lastname: Cohen
authors.forename: Nicolas
authors.initials: N
authors.lastname: Joly
mesh_terms: D000333:Aeromonas / Q000737:chemistry; D001884:Bordetella / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D004926:Escherichia coli / Q000737:chemistry; D048168:Escherichia coli K12 / Q000737:chemistry; D007711:Klebsiella pneumoniae / Q000737:chemistry; D008050:Lipid A / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009016:Moraxella / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D012475:Salmonella / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014733:Vibrio / Q000737:chemistry
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D009844:Oligosaccharides