Drug refractoriness of epileptiform activity in organotypic hippocampal slice cultures depends on the mode of provocation.
We previously reported that low Mg(2+) or 4-aminopyridine induced seizure-like events in organotypic hippocampal slice cultures (OHSCs) were resistant to standard antiepileptic drugs (Albus et al., 2008). We now show that high-frequency electrical stimulation induced ictal-like afterdischarges in OHSCs are effectively suppressed by the standard antiepileptic drugs phenytoin and carbamazepine. Thus, a single OHSC can express both pharmacoresistant and pharmacosensitive epileptiform activities.
Wahab
Abdul
A
Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Tucholskystrasse 2, Berlin, Germany.
Albus
Klaus
K
Heinemann
Uwe
U
D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D002220:Carbamazepine / Q000494:pharmacology*; D004558:Electric Stimulation; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D009924:Organ Culture Techniques; D010672:Phenytoin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000927:Anticonvulsants; D002220:Carbamazepine; D010672:Phenytoin