Molecular MR imaging of cancer gene therapy: ferritin transgene reporter takes the stage.
Molecular imaging using magnetic resonance (MR) imaging has been actively investigated and made rapid progress in the past decade. Applied to cancer gene therapy, the technique's high spatial resolution allows evaluation of gene delivery into target tissues. Because noninvasive monitoring of the duration, location, and magnitude of transgene expression in tumor tissues or cells provides useful information for assessing therapeutic efficacy and optimizing protocols, molecular imaging is expected to become a critical step in the success of cancer gene therapy in the near future. We present a brief overview of the current status of molecular MR imaging, especially in vivo reporter gene imaging using ferritin and other reporters, discuss its application to cancer gene therapy, and present our research of MR imaging detection of electroporation-mediated cancer gene therapy using the ferritin reporter gene.
Hasegawa
Sumitaka
S
Molecular Imaging Center, National Institute of Radiological Sciences, Inage-ku, Chiba, Japan. shase@nirs.go.jp
Furukawa
Takako
T
Saga
Tsuneo
T
D000818:Animals; D005293:Ferritins / Q000032:analysis* / Q000235:genetics; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009369:Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000628:therapy*
D005293:Ferritins