Strategies for three-dimensional particle tracking with holographic video microscopy.
The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties.
Cheong
Fook Chiong
FC
Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA.
Krishnatreya
Bhaskar Jyoti
BJ
Grier
David G
DG
D000465:Algorithms; D006696:Holography / Q000295:instrumentation* / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D053758:Nanoparticles; D012542:Scattering, Radiation