Selective imaging of nano-particle contrast agents by a single-shot x-ray diffraction technique.
Iron oxide nano-particles have very different x-ray diffraction properties from tissue. They can be clearly visualized against suppressed tissue background in a single-shot x-ray diffraction imaging technique. This technique is able to acquire both diffraction and absorption images from a single grating-modulated projection image through analysis in the spatial frequency domain. We describe the use of two orthogonal transmission gratings to selectively retain diffraction signal from iron oxide particles that are larger than a threshold size, while eliminating the background signal from soft tissue and bone. This approach should help the tracking of functionalized particles in cell labeling and targeted therapy.
Stein
Ashley F
AF
Imaging Physic Section, Translational Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Ilavsky
Jan
J
Kopace
Rael
R
Bennett
Eric E
EE
Wen
Han
H
D000818:Animals; D002645:Chickens; D003287:Contrast Media / Q000737:chemistry*; D005290:Ferric Compounds / Q000494:pharmacology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D053838:Scattering, Small Angle; D013977:Tibia / Q000033:anatomy & histology; D014921:Wings, Animal / Q000187:drug effects; D014961:X-Ray Diffraction / Q000379:methods*
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide