Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen.
A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).
Boontanom
Parima
P
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Siripattanapipong
Suradej
S
Mungthin
Mathirut
M
Tan-ariya
Peerapan
P
Leelayoova
Saovanee
S
D017931:DNA Primers; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics*; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013785:Thailand
D017931:DNA Primers; D005969:Glutamate Dehydrogenase