Development of single-tube mutiplex RT-PCR for dengue virus typing.
This study evaluated a single-tube multiplex RT-PCR with a primer focusing on nonstructural protein region 5 (NS5) and a highly conserved region for dengue virus serotyping. The method was compared with conventional PCR. This new method had a sensitivity of 96.7% and a specificity of 96.7% for disease detection. The new method also proved suitable for use in the field as it reduces time and decreases risk of contamination.
Maneekan
Pannamas
P
Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Jittmittraphap
Akanitt
A
Leaungwutiwong
Pornsawan
P
Kaewkangwal
Jaranit
J
Luplertlop
Natthanej
N
D003429:Cross Reactions; D017931:DNA Primers; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*
D017931:DNA Primers