Differential gene expression from midguts of refractory and susceptible lines of the mosquito, Aedes aegypti, infected with Dengue-2 virus.
Suppressive subtractive hybridization was used to evaluate the differential expression of midgut genes of feral populations of Aedes aegypti (Diptera: Culicidae) from Colombia that are naturally refractory or susceptible to Dengue-2 virus infection. A total of 165 differentially expressed sequence tags (ESTs) were identified in the subtracted libraries. The analysis showed a higher number of differentially expressed genes in the susceptible Ae. aegypti individuals than the refractory mosquitoes. The functional annotation of ESTs revealed a broad response in the susceptible library that included immune molecules, metabolic molecules and transcription factors. In the refractory strain, there was the presence of a trypsin inhibitor gene, which could play a role in the infection. These results serve as a template for more detailed studies aiming to characterize the genetic components of refractoriness, which in turn can be used to devise new approaches to combat transmission of dengue fever.
Barón
Olga L
OL
Centro lnternacional de Entrenamiento e Investigaciones Médicas, Cali, Colombia.
Ursic-Bedoya
Raul J
RJ
Lowenberger
Carl A
CA
Ocampo
Clara B
CB
D000330:Aedes / Q000378:metabolism* / Q000821:virology*; D000818:Animals; D003716:Dengue Virus / Q000502:physiology*; D004064:Digestive System / Q000378:metabolism*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D015723:Gene Library