title: Small peptide (P-15) bone substitute efficacy in a rabbit cancellous bone model.
abstract: P-15 is a synthetic 15-amino acid residue identical to the cell binding domain of type I collagen. P-15 can be adsorbed onto anorganic bovine bone mineral (ABM) and will enhance cell attachment and subsequent cell activation. Although ABM/P-15 has been studied as a bone graft substitute in the oral cavity, its use in orthopedic models has been limited. Thus, this study investigated the efficacy of ABM/P-15 treatment in a rabbit model of long bone cancellous healing. Defects were created in the distal femurs and proximal medial tibiae of rabbits and were filled with either ABMP/P-15 suspended in hydrogel, ABM alone suspended in hydrogel, hydrogel carrier alone, or no graft material. Rabbits were sacrificed at 1, 2, 4, or 8 weeks postsurgery, and the femurs and tibiae were harvested. Histomorphometric analyses indicated that defects treated with ABM/P-15 had significantly larger areas of new bone formation than the other three treatments at 2 and 8 weeks postsurgery. ABM/P-15 treated defects also had significantly more bone growth than defects left empty or filled with ABM alone at 4 weeks postsurgery. Furthermore, histological examination did not reveal acute inflammatory infiltrate cells in any of the treatment conditions. These results are consistent with the findings of ABM/P-15 use in human oral-maxillofacial studies and in large animal spine fusion models.
authors.affiliation: The Spine Center, Department of Orthopedics, University of Colorado, Denver, Colorado.
authors.forename: Emily M
authors.initials: EM
authors.lastname: Lindley
authors.affiliation: Faculty of Medicine, University of Coimbra, Portugal.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Guerra
authors.affiliation: Implant Center of the Palm Beaches, Boca Raton, Florida.
authors.forename: Jack
authors.initials: J
authors.lastname: T Krauser
authors.affiliation: Faculty of Medicine, University of Coimbra, Portugal.
authors.forename: Sergio M
authors.initials: SM
authors.lastname: Matos
authors.affiliation: The Spine Center, Department of Orthopedics, University of Colorado, Denver, Colorado.
authors.forename: Evalina L
authors.initials: EL
authors.lastname: Burger
authors.affiliation: The Spine Center, Department of Orthopedics, University of Colorado, Denver, Colorado.
authors.forename: Vikas V
authors.initials: VV
authors.lastname: Patel
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000627:therapeutic use; D003094:Collagen / Q000627:therapeutic use*; D005269:Femur / Q000254:growth & development / Q000293:injuries / Q000601:surgery; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020007:Implants, Experimental / Q000592:standards*; D010446:Peptide Fragments / Q000627:therapeutic use*; D011817:Rabbits; D013977:Tibia / Q000254:growth & development / Q000293:injuries / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D018786:Bone Substitutes; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003094:Collagen