Monocyte differentiation toward regulatory dendritic cells is not affected by respiratory syncytial virus-induced inflammatory mediators.
Airway epithelial cells were shown to drive the differentiation of monocytes into dendritic cells (DCs) with a suppressive phenotype. In this study, we investigated the impact of virus-induced inflammatory mediator production on the development of DCs. Monocyte differentiation into functional DCs, as reflected by the expression of CD11c, CD123, BDCA-4, and DC-SIGN and the capacity to activate T cells, was similar for respiratory syncytial virus (RSV)-infected and mock-infected BEAS-2B and A549 cells. RSV-conditioned culture media resulted in a partially mature DC phenotype, but failed to up-regulate CD80, CD83, CD86, and CCR7, and failed to release proinflammatory mediators upon Toll-like receptor (TLR) triggering. Nevertheless, these DCs were able to maintain an antiviral response by the release of Type I IFN. Collectively, these data indicate that the airway epithelium maintains an important suppressive DC phenotype under the inflammatory conditions induced by infection with RSV.
Sluijs
Koenraad F van der
KF
Department of Respiratory Medicine, University Hospital of Berne, Switzerland. kvandersluijs@amc.uva.nl
Obregon
Carolina
C
Geiser
Thomas K
TK
Mühlemann
Kathrin
K
Nicod
Laurent P
LP
D015703:Antigens, CD / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen / Q000096:biosynthesis; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007249:Inflammation / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D010641:Phenotype; D054400:Receptors, CCR7 / Q000096:biosynthesis; D012136:Respiratory Syncytial Viruses / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D000097802:CD83 Antigen
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D007136:Immunoglobulins; D018836:Inflammation Mediators; D008562:Membrane Glycoproteins; D054400:Receptors, CCR7; D051193:Toll-Like Receptors; D000097802:CD83 Antigen