[Comparative analysis of respiratory activity in the wild type strain of Neurospora crassa and its photoreceptor complex mutants].
Cell respiratory activity of protoplasts obtained from the wild type of Neurospora crassa and photoreceptor complex WCC--white collar 1 (wc-1) and white collar 2 (wc-2)--mutants of Neurospora crassa strains was investigated. Respiration inhibition by KCN in the presence of 25 mM succinate was similar in all strains and did not exceed 83-85% against control. The significant induction of KCN-resistant respiratory pathway occurred under 1% glucose oxidation in wc-1 and wc-2 mutants if compared with the wild type strains. The inhibitors of the main (cytochrome) pathway of electron transfer in mitochondria-1 mM KCN and antimycin A (4 microg/ml)--blocked the respiration rate of the protoplasts from N. crassa wild type by 75%, while the cell respiration of wc-1 and wc-2 strains was suppressed by approximately 50%. The specific inhibitor of alternative oxidase--10 mM salicylhydroxamic acid (SHAM)-in combination with the blockers of mitochondrial electron transfer chain caused the total suppression of respiratory activity of protoplasts in all studied strains. It is supposed that an increase of KCN-resistance in WCC mutants under glucose oxidation is connected with alternative oxidase activation as the result of failure in reception and signal transduction of active oxygen species.
Isakova
E P
EP
Deriabina
Iu I
IuI
Gessler
N N
NN
Belozerskaia
T A
TA
Rabinovich
Ia M
IaM
D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins; D011190:Potassium Cyanide / Q000494:pharmacology; D011523:Protoplasts / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012457:Salicylamides / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D019802:Succinic Acid / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099188:Alternative Oxidase
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D005947:Glucose; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011190:Potassium Cyanide; D017382:Reactive Oxygen Species; D012457:Salicylamides; D019802:Succinic Acid; D014157:Transcription Factors; D000099188:Alternative Oxidase; C105589:wc-1 protein, Neurospora crassa; C103772:white collar 2 protein, Neurospora; C005703:salicylhydroxamic acid