Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection.
This work presents a comparison of convective interaction media (CIM) and controlled pore glass (CPG) as solid supports for immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs. A flow-injection manifold with highly sensitive thermal lens spectrometric detection was used for this purpose. Using beta-lactoglobulin, a milk allergen, as a model analyte, CIM disc supports had a higher linear range (0.2-3.5 microg L(-1)), better reproducibility (intra-day RSD = 1%, inter-day RSD = 10%), lower consumption of reagents, and better immunocolumn stability (1 month, over 240 injections of substrate), while providing comparable LODs (0.1 microg L(-1)). Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening of allergens in foodstuffs with sample throughput of up to eight samples per hour.
Cevdek
Ana
A
Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, P.O. Box 301, 5001-SI Nova Gorica, Slovenia.
Franko
Mladen
M
D000485:Allergens / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D005898:Glass / Q000737:chemistry*; D007074:Immunoglobulin G / Q000276:immunology; D007782:Lactoglobulins / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection
D000485:Allergens; D000906:Antibodies; D007074:Immunoglobulin G; D007782:Lactoglobulins