Behavioral alterations and pro-oxidant effect of a single ketamine administration to mice.
A growing body of evidence has pointed to the ionotropic glutamate N-methyl-d-aspartate receptor (NMDA) as an important player in the etiology of psychopathologies, including anxiety and major depression. Clinical findings suggest that ketamine may be used for the treatment of major depression. There is evidence that reactive oxygen species also play an important role in the pathogenesis of many diseases, particularly those which are neurological and psychiatric in nature. This study examined the behavioral and oxidative stress alterations after a single administration of ketamine (5, 10 and 20mg/kg i.p.) in mice. Ketamine presented a significant anxiogenic effect in the elevated plus-maze model of anxiety, also increasing locomotor activity. In the forced swimming and tail suspension tests, a significant decrease in immobility time after ketamine administration was observed. In addition to the behavioral changes induced by ketamine, this drug also increased lipid peroxidation, nitrite content and catalase activity, while decreased GSH levels in mice prefrontal cortex. In conclusion, our results confirm the antidepressant effects of ketamine, also showing a pro-oxidant effect of this drug.
da Silva
Francisca Charliane Carlos
FC
Laboratory of Neuropharmacology, Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil. charliane.enf@bol.com.br
do Carmo de Oliveira Cito
Maria
M
da Silva
Maria Izabel Gomes
MI
Moura
Brinell Arcanjo
BA
de Aquino Neto
Manuel Rufino
MR
Feitosa
Mariana Lima
ML
de Castro Chaves
Raquell
R
Macedo
Danielle Silveira
DS
de Vasconcelos
Silvania Maria Mendes
SM
de França Fonteles
Marta Maria
MM
de Sousa
Francisca Cléa Florenço
FC
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D019417:Hindlimb Suspension / Q000379:methods; D007649:Ketamine / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D013550:Swimming / Q000523:psychology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018691:Excitatory Amino Acid Antagonists; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances; D007649:Ketamine; D002374:Catalase; D005978:Glutathione