Effect of flavonoids on the Abeta(25-35)-phospholipid bilayers interaction.
Amyloid-beta peptide (Abeta) is the major component of amyloid deposits found in the brain tissue of Alzheimer patients. The tendency of amyloid peptide to form amyloid plaques is known to be related to the features of the plasma membrane. Flavonoids, a group of naturally occurring molecules, exert beneficial properties to human health thanks to their antioxidant property; this property depends on their capacity to interact and permeate the cell membrane lipid bilayer. In the present research we report an Electron Paramagnetic Resonance (EPR) investigation of 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) membranes interacting with the beta-amyloid fragment Abeta(25-35), in the presence of flavonoids rutin, quercetin, naringin and naringenin. Our results, evidencing a flavonoid-dependent rigidifying effect of the bilayer, may provide the molecular basis to explain the known neuroprotective effect of flavonoid compounds.
Tedeschi
Annamaria
A
Department of Pharmaceutical Sciences, University of Salerno, Via Don Melillo 11C, I-84084 Fisciano, Italy.
D'Errico
Gerardino
G
Lauro
Maria Rosaria
MR
Sansone
Francesca
F
Di Marino
Sara
S
D'Ursi
Anna Maria
AM
Aquino
Rita Patrizia
RP
D016229:Amyloid beta-Peptides / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D013329:Structure-Activity Relationship
D016229:Amyloid beta-Peptides; D005419:Flavonoids; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010743:Phospholipids; C074636:amyloid beta-protein (25-35)