title: Chromosomal conservation and sequence diversity of ribosomal RNA genes of two distant Oryza species.
abstract: Contrary to the chromosomal polymorphism of 45S ribosomal genes (45S rDNA) loci in other Oryza species, each of Oryza australiensis and Oryza brachyantha has only one 45S rDNA locus at the most conserved position of 45S rDNAs in Oryza. O. australiensis and O. brachyantha are known phylogenetically distant and have extremely different genome sizes among diploid Oryza species. This study reveals that the sequences and organizations of intergenic spacer (IGS) for 45S rDNA of both O. australiensis and O. brachyantha are different from other Oryza species. The IGS of O. australiensis contains 13 tandem repeats and only one transcriptional initiation site, while there are four tandem repeats and three transcriptional initiation sites in the IGS of O. brachyantha. Our results suggest different evolution processes of orthologous rDNA loci in the genus Oryza. Here we also demonstrate an efficient strategy to study locus-specific IGS before whole genome sequences data are available.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Kwei-Duan
authors.initials: KD
authors.lastname: Chang
authors.forename: Shao-An
authors.initials: SA
authors.lastname: Fang
authors.forename: Fang-Chi
authors.initials: FC
authors.lastname: Chang
authors.forename: Mei-Chu
authors.initials: MC
authors.lastname: Chung
mesh_terms: D015139:Blotting, Southern; D032461:Chromosomes, Plant / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D019143:Evolution, Molecular; D040461:Gene Components; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics; D024363:Transcription Initiation Site
chemical_list: D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal