title: Conformational organizations of G-quadruplexes composed of d(G(4)T(n))(3)G(4).
abstract: Structural polymorphism is one of the important issues with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and their physical property in nano-material. A series of oligonucleotides with four repeat guanines sequence [d(G(4)T(n))(3)G(4) (n=1-6)] were designed. In this study, the effects of loop length on the formation of structures of G-quadruplex were investigated through the result of CD (circular dichroism) and 20% non-denatured polyacrylamide gel electrophoresis. Our studies demonstrate that the length of loop in 100mM KCl solution could predict the conformation of G-quadruplex.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
authors.forename: Wan Chi
authors.initials: WC
authors.lastname: Wong
authors.forename: Jinyi
authors.initials: J
authors.lastname: Zhuang
authors.forename: Selina Ling Ling
authors.initials: SL
authors.lastname: Ng
authors.forename: Lilian Li Lin
authors.initials: LL
authors.lastname: New
authors.forename: Shuhui
authors.initials: S
authors.lastname: Hiew
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Guo
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Yang
authors.forename: Tianhu
authors.initials: T
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D006147:Guanine