title: Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils.
abstract: A collection of 38 bacteria was obtained by enrichment cultivation from oil-contaminated soils of an oil field in Daqing, China. Twenty-two strains could utilize diesel oil as the sole source of carbon and energy, and 11 strains could degrade the total petroleum hydrocarbons (TPHs) of diesel oil by more than 70% in 7d. Phylogenetically, 19 of the bacteria related to Bacillus species. About 87.5% TPHs of crude oil were degraded by a consortium of seven strains. Denaturing gradient gel electrophoresis analysis suggested that five of the strains persisted throughout the degradation process. The collection of isolated bacteria might be a useful resource for bioremediation of oil-contaminated soils and biotreatment of oil wastewater.
authors.affiliation: MOE Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lixue
authors.initials: L
authors.lastname: Gai
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Hou
authors.forename: Chunyu
authors.initials: C
authors.lastname: Yang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Zhongguo
authors.initials: Z
authors.lastname: Wang
authors.forename: Baiping
authors.initials: B
authors.lastname: Sun
authors.forename: Xiaofei
authors.initials: X
authors.lastname: He
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Tang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D005742:Gasoline / Q000032:analysis; D006838:Hydrocarbons / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D005742:Gasoline; D006838:Hydrocarbons; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants