Differential gene and protein expression in primary gastric carcinomas and their lymph node metastases as revealed by combined cDNA microarray and tissue microarray analysis.
OBJECTIVE
To gain insight into the molecular events of lymph node metastasis of human gastric carcinoma.


METHODS
The gene expression profile of five matched primary gastric carcinomas and their lymph node metastases was analyzed by complementary DNA (cDNA) microarray. Differential genes were identified in the metastatic and corresponding primary tumor pairs. Among the differentially expressed genes, carbonic anhydrase II (CAII) and insulin-like growth factor binding protein 4 (IGFBP 4) genes were detected by RT-PCR. CTTN protein expression was examined by tissue microarray.


RESULTS
There was a high expression (over twofold) of 44 genes and a low expression (under twofold) of 32 genes in lymph node metastasis compared with primary gastric carcinoma, respectively. CAII mRNA was downregulated and IGFBP 4 mRNA was upregulated in paired lymph node metastases of gastric carcinomas. The overexpression of CTTN protein was related to the lymph node metastasis and the clinical stage of gastric carcinomas.


CONCLUSIONS
This study showed that there is a low expression of genes relative to growth signal and immune response in lymph node metastases, and a high expression of genes relative to growth factor, cell cycle, cell motility and adhesion in lymph node metastases compared with primary gastric carcinomas. The expression of CTTN was related to the invasion and metastasis of gastric cancer.
Xie
Hai Long
HL
Cancer Research Institute, Medical College of University of South China, Hengyang, Hunan Province, China.
Li
Zhi Yu
ZY
Gan
Run Liang
RL
Li
Xiao Jie
XJ
Zhang
Qing Li
QL
Hui
Mei
M
Zhou
Xiu Tian
XT
D024402:Carbonic Anhydrase II / Q000235:genetics / Q000378:metabolism; D051356:Cortactin / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018974:Insulin-Like Growth Factor Binding Protein 4 / Q000235:genetics / Q000378:metabolism; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011379:Prognosis; D040081:Protein Array Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D015854:Up-Regulation / Q000235:genetics
C495868:CTTN protein, human; D051356:Cortactin; D018974:Insulin-Like Growth Factor Binding Protein 4; D024402:Carbonic Anhydrase II