Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells.
Generation of induced pluripotent stem cells from somatic cells using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient and time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation of mouse induced pluripotent stem cells. This optimization of the culture medium is sensitive to the presence of Myc in the reprogramming factors. Moreover, we could reprogram meningeal cells using only two factors Oct4/Klf4. Therefore, iSF1 represents a basal medium that may be used for mechanistic studies and testing new reprogramming approaches.
Chen
Jiekai
J
Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China.
Liu
Jing
J
Han
Qingkai
Q
Qin
Dajiang
D
Xu
Jianyong
J
Chen
You
Y
Yang
Jiaqi
J
Song
Hong
H
Yang
Dongshan
D
Peng
Meixiu
M
He
Wenzhi
W
Li
Ronghui
R
Wang
Hao
H
Gan
Yi
Y
Ding
Ke
K
Zeng
Lingwen
L
Lai
Liangxue
L
Esteban
Miguel A
MA
Pei
Duanqing
D
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000494:pharmacology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D008822:Mice, Transgenic
D016895:Culture Media, Serum-Free; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4