title: Dynamic modulation of cytoskeleton during in vitro maturation in human oocytes.
abstract: OBJECTIVE
  To investigate the role of cytoskeleton in several important dynamic events during in vitro maturation of human oocytes.
  
  
  METHODS
  Human germinal vesicle stage oocytes were divided randomly into control and study groups. After cultured for 24 hours, chromatin state and position, spindle formation and migration, cortical granules, and mitochondria distribution were evaluated.
  
  
  RESULTS
  In colchicine group, spindles did not form. Cortical granules migrated to the cortex but mitochondria maintained the peripheral distribution pattern in most of the oocytes. In cytochalasin B group, the migration of spindle and chromosomes to the cortex was prohibited. Microfilaments disruption influenced cortical granules migration but not redistribution of mitochondria.
  
  
  CONCLUSIONS
  Meiosis progression could not go beyond metaphase I stage when microtubule or microfilament polymerization was prohibited in human oocytes. The migration of cortical granules to the cortex and redistribution of mitochondria to the inner cytoplasm were mediated by microfilaments and microtubules, respectively.
authors.affiliation: Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Key Laboratory of Reproductive Medicine, Shandong Province, Jinan, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Huai L
authors.initials: HL
authors.lastname: Feng
authors.forename: Jun H
authors.initials: JH
authors.lastname: Yan
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Shui Y
authors.initials: SY
authors.lastname: Ma
authors.forename: Zi J
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D017077:Culture Media, Conditioned; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008540:Meiosis / Q000187:drug effects* / Q000502:physiology*; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology; D015588:Observer Variation; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D011336:Probability; D011897:Random Allocation
chemical_list: D017077:Culture Media, Conditioned; D003571:Cytochalasin B; D003078:Colchicine