Trypanosoma brucei: immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis.
Trypanosoma brucei is the etiological agent responsible for African trypanosomiasis, an infectious pathology which represents a serious problem of public health and economic losses in Sub-Saharan Africa. As one of the foremost neglected illnesses, few resources have been available for the development of vaccines or new drugs, in spite of the current therapeutical drugs showing little efficiency and high toxicity. Hence, it is obviously important to widen effective therapeutics and preventive strategies against African trypanosomiasis. In this work, we use the DNA vaccine model to evaluate immunisation effectiveness in mice challenged with Trypanosoma brucei brucei. We demonstrate that Balb/C mice immunised intramuscularly with a single dose of a DNA plasmid encoding a bloodstream-stage specific invariant surface glycoprotein (ISG) are partially protected from a lethal dose of T. b. brucei. Interestingly, the surviving animals show high levels of IgG2a anti-trypanosoma antibodies, suggesting that the Th1 response profile seems important for the induced mechanisms of immune protection.
Lança
Andreia Sofia Cruz
AS
Unidade de Ensino e Investigação de Clínica das Doenças Tropicais, Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Portugal.
de Sousa
Karina Pires
KP
Atouguia
Jorge
J
Prazeres
Duarte Miguel F
DM
Monteiro
Gabriel Amaro
GA
Silva
Marcelo Sousa
MS
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015153:Blotting, Western; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000517:prevention & control*; D019444:Vaccines, DNA
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA