[Quantitative determination of podophyllotoxin in dermal and blood microdialysis samples of rats by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
To develop and validate a sensitive method for quantitative analysis of podophyllotoxin in blood and dermal microdialysis samples of rats based on liquid chromatography-tandem mass spectrometry (UFLC-MS-MS).


METHODS
The microdialysis samples were prepared by liquid-liquid extraction using ethyl acetate with etoposide as the internal standard (IS). Podophyllotoxin was separated with an Agilent ZORBAX XDB-C18 column (2.1 mmx50 mm, 3.5 microm). The mobile phase consisted of acetonitrile: 10 mmol/L ammonium acetate (40:60, V/V) at a flow rate of 0.3 ml/min and the analysis was performed at the ambient temperature. The UFLC-MS/MS system was operated in the mode of multiple reaction monitoring using the electrospray ionization technique in positive mode.


RESULTS
Podophyllotoxin and etoposide responses were optimized at the transitions m/z 432.7-->397.3 and 589.5-->229.5, respectively. Calibration curves were linear over the range 2.0-1000 ng/ml. The lowest limits of quantification and detection values were 2.0 ng/ml and 0.7 ng/ml, respectively. The inter- and intra-day precision and accuracy were both less than 15%.


CONCLUSIONS
This selective and sensitive method can be used to quantity podophyllotoxin in the blood and dermal microdialysates of rats.
Wang
Xiao-po
XP
Department of Dermatology, Nanfang Hospital, Hygiene Detection Center, Southern Medical University, Guangzhou 510515, China. butterfly.bow@163.com
Zeng
Kang
K
Li
Guo-feng
GF
Chen
Ling-yun
LY
Ma
An-de
AD
Wang
Lin
L
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D017551:Microdialysis / Q000379:methods*; D011034:Podophyllotoxin / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D012680:Sensitivity and Specificity; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D011034:Podophyllotoxin