Automated lab-scale production of PVA/PEG-enzyme immobilisates.
Entrapment of biocatalysts by cryogelation is a gentle method to extend the scope of biocatalysis. To foster the use of this versatile method we devised an automated injector for the production of PVA/PEG beads. The device consists of a thermostated reservoir connected to a programmable injector nozzle and an agitated receiving bath for the droplets. This lab-scale production unit yields up to 1500 beads with immobilized enzyme per minute with a narrow size distribution and good roundness.
Bieler
Nora C
NC
Institute of Chemistry, Enzyme Technology, TU Berlin, Berlin, Germany.
Ansorge-Schumacher
Marion B
MB
Greiner
Lasse
L
D057205:Automation, Laboratory / Q000379:methods*; D001709:Biotechnology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D015203:Reproducibility of Results; D013696:Temperature
D004800:Enzymes, Immobilized; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol