title: No effect in combining chondrogenic predifferentiation and mechanical cyclic compression on osteochondral constructs stimulated in a bioreactor.
abstract: Traumatic and degenerative osteochondral lesions are a common problem in orthopaedic surgery. The concept of tissue engineering represents the possibility of a promising therapeutical approach. The purpose of this study has been to improve the characteristics of osteochondral grafts consisting of a human certified collagen I-bone hybrid matrix seeded with human bone marrow stromal cells and stimulated in a custom-made biomechanoreactor. This study was undertaken as a follow-up to our prior studies. Based on our established system, we added chondrogenic growth factors (IGF-1 and TGF-beta(2)) and evaluated their effect on chondrogenic differentiation. Constructs were stimulated for 14, 21 and 28 days respectively by different protocols, including cyclic mechanical stimulation, hormonal stimulation or a combination of both. More than 70% of the cells were viable throughout the entire experimental period. Histological analysis revealed a homogeneous distribution of cells in a cartilage-like matrix organization. Immunohistological collagen II staining was positive irrespective of stimulation manner and time. Levels of DNA and glycosaminoglycans, having been normalized to DNA, did not change. Analysis of the biomechanical stiffness after 14 days showed increased stiffness in the hormonally and mechanically stimulated group compared to the static group. Stimulation time did not have a significant influence. The media supplements to foster the quality of the tissue tested here did not show any progress in our system. We conclude that cyclic compression enhances matrix stiffness, but stimulation time should be kept short and growth factors should be left out in this system with regard to clinical applicability and financial concerns.
authors.affiliation: Department of Orthopaedic Surgery, Hannover Medical School, Germany. stefan.budde@ddh-gruppe.de
authors.forename: S
authors.initials: S
authors.lastname: Budde
authors.forename: M
authors.initials: M
authors.lastname: Jagodzinski
authors.forename: M
authors.initials: M
authors.lastname: Wehmeier
authors.forename: C
authors.initials: C
authors.lastname: Hurschler
authors.forename: B
authors.initials: B
authors.lastname: Richter
authors.forename: M
authors.initials: M
authors.lastname: Broese
authors.forename: F
authors.initials: F
authors.lastname: Paulsen
authors.forename: T
authors.initials: T
authors.lastname: Tschernig
authors.forename: C
authors.initials: C
authors.lastname: Krettek
authors.forename: C
authors.initials: C
authors.lastname: Haasper
mesh_terms: D001696:Biomechanical Phenomena; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D001842:Bone and Bones; D002356:Cartilage / Q000254:growth & development*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D020219:Chondrogenesis; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005109:Extracellular Matrix / Q000737:chemistry; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D013314:Stress, Mechanical; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D004247:DNA