Label-free DNA detection platform based on atomic force microscopy visualisation: characterising the molecular-recognition-triggered conformational changes of an immobilised receptor oligonucleotide probe.
Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, DNA hybridisation assay platform.
Nakano
Koji
K
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakano@cstf.kyushu-u.ac.jp
Yamanouchi
Hirokazu
H
Yoshinaga
Hisao
H
Soh
Nobuaki
N
Imato
Toshihiko
T
D004247:DNA / Q000032:analysis*; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
D015345:Oligonucleotide Probes; D012822:Silicon Dioxide; D004247:DNA