title: The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of erythropoietin and dimethylsulfoxide in the recovery from ischemia-reperfusion injury in an experimental rat adnexal torsion model.
  
  
  METHODS
  Thirty-six Wistar-albino rats were divided into six groups. Except for the sham operation group, all groups were subjected to left unilateral adnexal torsion for 3h. Erythropoietin and dimethylsulfoxide were intraperitoneally administered 30min before the detorsion operation. Malondialdehyde and nitric oxide levels were detected from both the plasma and the tissue samples. The sections of the tissues were evaluated histologically. The results were analyzed by a one-way analysis of the variance (ANOVA) followed by the Duncan test for multiple comparisons using computer software, SPSS Version 15.0 for Windows.
  
  
  RESULTS
  This study demonstrated that dimethylsulfoxide and erythropoietin pretreatment attenuated ischemia-reperfusion-induced lipid peroxidation, prevented post-ischemic ovarian injury and helped to maintain the ovarian morphology. Malondialdehyde levels of plasma and ovary were higher in the torsion and detorsion groups than the sham group. This showed that ischemia-reperfusion had caused lipid peroxidation of the ovarian tissue, thus leading to oxidative damage. One of the major findings of this study is that malondialdehyde was significantly decreased in the plasma of rats who were pre-treated with dimethylsulfoxide and erythropoietin before detorsion. This suggests that dimethylsulfoxide and erythropoietin might prevent oxidative damage in ovarian ischemia-reperfusion injury. Histological examination confirmed that reperfusion caused more detrimental effects than only ischemia, which could be at least partially prevented by dimethylsulfoxide and erythropoietin administration prior to detorsion.
  
  
  CONCLUSIONS
  Erythropoietin and dimethylsulfoxide may have beneficial effects in ischemia-reperfusion injury in ovarian torsion.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Mustafa Kemal University, Antakya, Hatay, Turkey. yasarergun@yahoo.com
authors.forename: Yasar
authors.initials: Y
authors.lastname: Ergun
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koc
authors.forename: Kenan
authors.initials: K
authors.lastname: Dolapcioglu
authors.forename: Yesim
authors.initials: Y
authors.lastname: Akaydin
authors.forename: Gokhan
authors.initials: G
authors.lastname: Dogruer
authors.forename: Tunay
authors.initials: T
authors.lastname: Kontas
authors.forename: Tolunay
authors.initials: T
authors.lastname: Kozlu
authors.forename: Erdogan
authors.initials: E
authors.lastname: Aslan
mesh_terms: D000291:Adnexal Diseases / Q000209:etiology; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D004921:Erythropoietin / Q000627:therapeutic use*; D005260:Female; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010049:Ovarian Diseases / Q000473:pathology / Q000517:prevention & control*; D010053:Ovary / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications
chemical_list: D004921:Erythropoietin; D009569:Nitric Oxide; D008315:Malondialdehyde; D004121:Dimethyl Sulfoxide