Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx).
Phospholipid hydroperoxide glutathione peroxidase (PHGPx), as a ubiquitous antioxidant enzyme in the glutathione peroxidases (GPx) family, plays multiple roles in organisms. However, there is very little information on PHGPx in goats (Capra hircus). In this study, a full-length cDNA was cloned and characterized from Taihang black goat testes. The 844 bp cDNA contains an open reading frame (ORF) of 597 bp. The goat PHGPx nucleotide sequence contains a selenocysteine (sec) codon TGA(244-246), two potential start codons ATG(20-22) and ATG(108-110), a polyadenylation signal AATAAA(813-818) and selenocysteine insertion sequence (SECIS) motif AUGA(688-691), UGA(729-731) and AAA(703-705). As a selenoprotein, the active-site motifs and GPx family signature motifs LAFPCNQF(101-108) and WNFEK(165-170) were also found. The order of PHGPx mRNA expression levels was: testes >> heart > brain > epididymis > kidney > liver > lung > spleen > muscle. Real-time PCR and immunohistochemistry results revealed similar expression differences in different age testes, with high expression levels during adolescence. Immunofluorescence results suggested that PHGPx mainly expressed in Leydig cells and spermatids in mature goat testes.
Shi
Li-guang
LG
College of Animal Science and Veterinary Medicines, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Xun
Wen-juan
WJ
Yue
Wen-bin
WB
Zhang
Chun-xiang
CX
Ren
You-she
YS
Wang
Qian
Q
Wu
Xiao-ying
XY
Shi
Lei
L
Yang
Ru-jie
RJ
Lei
Fu-lin
FL
D000818:Animals; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006041:Goats / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D016366:Open Reading Frames / Q000235:genetics; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D016133:Polymerase Chain Reaction; D013737:Testis / Q000201:enzymology*
D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase