Phytoglycoprotein (75 kDa) inhibits expression of interleukin-1beta stimulated by DEHP in human mast cells.
The purpose of this study is to investigate the inhibitory effect of a glycoprotein (CTB glycoprotein, 75 kDa) isolated from Cudrania tricuspidata Bureau (CTB) on the di-(2-ethylhexyl)phthalate (DEHP) induced expression of allergic-inflammation-related mediators in human mast cells. The changes on the levels of intracellular reactive oxygen species (ROS), mitogen-activated protein kinase (MAPK), transcription factor [nuclear factor (NF)-kappaB], and allergic inflammatory mediators [cyclooxygenase (COX)-2 and interleukin (IL)-1beta] were evaluated using Western blot and RT-PCR. Our results showed that the CTB glycoprotein in the presence of DEHP inhibits the production of intracellular ROS, the phosphorylation of ERK1/2, and p38 MAPK in HMC-1 cells. In addition, the CTB glycoprotein has suppressive effects on the transcriptional activation of NF-kappaB, and on the expression levels of COX-2 and IL-1beta in DEHP-treated HMC-1 cells. In conclusion, the CTB glycoprotein has a strong anti-inflammatory effect on the activities of allergic inflammatory mediators indirectly caused by DEHP in HMC-1 cells.
Oh
Phil-Sun
PS
Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Yongbong-Dong, Gwang-ju, South Korea.
Lim
Kwang
K
Lim
Kye-Taek
KT
D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D029586:Moraceae / Q000737:chemistry; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D006023:Glycoproteins; D053583:Interleukin-1beta; D016328:NF-kappa B; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D004051:Diethylhexyl Phthalate; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases