title: TP53 gene mutations in prostate cancer progression.
abstract: BACKGROUND
  We assessed the predictive value of TP53 mutations and prostate-specific antigen (PSA) for tumor progression in prostate cancer (PCa) patients.
  
  
  METHODS
  Ninety tumor tissue samples of patients with PCa from radical prostatectomy were used. Tumor progression was estimated biochemically by the PSA level (> 0.2 microg/l) or by detection of metastases. Screening for TP53 mutations was performed by temperature gradient gel electrophoresis (TGGE) in exon-specific manner. Follow-up data were collected from medical protocols. Statistical analysis was performed by uni- and multivariate techniques.
  
  
  RESULTS
  In 32 out of 90 patients (35.6%), TP53 mutations were detected. Thirteen out of 32 patients (40.6%) with TP53 mutations and nine out of 58 patients (15.5%) with TP53 wild-type showed tumor progression after 25 and 45 months, respectively.
  
  
  CONCLUSIONS
  TP53 mutations in exon 7 and exon 8 are factors of tumor progression in PCa. Their contribution to tumor recurrence is more significant than tumor stage and pretherapeutic PSA level.
authors.affiliation: HELIOS Hospital, Department of Urology, Bad Saarow, Germany. thorsten.ecke@helios-kliniken.de
authors.forename: Thorsten H
authors.initials: TH
authors.lastname: Ecke
authors.forename: Horst H
authors.initials: HH
authors.lastname: Schlechte
authors.forename: Katrin
authors.initials: K
authors.lastname: Schiemenz
authors.forename: Markus D
authors.initials: MD
authors.lastname: Sachs
authors.forename: Severin V
authors.initials: SV
authors.lastname: Lenk
authors.forename: Birgit D
authors.initials: BD
authors.lastname: Rudolph
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Loening
mesh_terms: D004252:DNA Mutational Analysis; D018450:Disease Progression; D005091:Exons; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009362:Neoplasm Metastasis; D017430:Prostate-Specific Antigen / Q000737:chemistry; D011468:Prostatectomy / Q000379:methods; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D012307:Risk Factors; D013696:Temperature
chemical_list: D017430:Prostate-Specific Antigen