Effect of Agaricus blazei Murill on the pulmonary tissue of animals with streptozotocin-induced diabetes.
The present study was designed to evaluate the oxidative stress as well as the therapeutic effect of Agaricus blazei Muril (A. Blazei) in rats with streptozotocin-induced diabetes. We used 25 Wistar rats, and DM was induced by injecting streptozotocin (70 mg/Kg i.p.). Agaricus blazei Muril was administered daily starting 40 days after disease onset. A. Blazei was tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipoperoxidation (LPO), and superoxide dismutase (SOD), catalase, and glutathione peroxidase activities were measured in the pulmonary tissue, as well as the presence of inducible nitric oxide synthase (iNOS), through immunohistochemistry. An anatomopathologic study was also performed. Phytochemical screening of A. Blazei detected the presence of alkaloids and saponins. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hipoxanthine/xanthine oxidase assays. Pulmonary LPO increased in diabetic animals (0.43 +/- 0.09; P < .001) as compared to the control group (0.18 +/- 0.02), followed by a reduction in the A. Blazei-treated group (0.33 +/- 0.04; P < .05). iNOS was found increased in the lung in diabetic rats and reduced in the A. Blazei-treated group. The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. The A. Blazei treatment effectively reduced the oxidative stress and contributed to tissue recovery.
Di Naso
Fábio Cangeri
FC
Laboratory of Experimental Hepatology and Physiology, Porto Alegre Clinical Hospital, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
de Mello
Rodrigo Noronha
RN
Bona
Sílvia
S
Dias
Alexandre Simões
AS
Porawski
Marilene
M
Ferraz
Alexandre de Barros Falcão
Ade B
Richter
Marc François
MF
Marroni
Norma Possa
NP
D000364:Agaricus / Q000737:chemistry; D000470:Alkaloids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000628:therapy*; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D000470:Alkaloids; D000975:Antioxidants; D012503:Saponins; D014280:Triglycerides; D002784:Cholesterol; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase