Replication and partitioning of the broad-host-range plasmid RK2.
The broad-host-range plasmid RK2 has been a model for studying DNA metabolism in bacteria for many years. It is used as a vector allowing genetic manipulations in numerous bacterial species. The RK2 genome encodes several genes providing the plasmid with diverse functions allowing for its stable maintenance in a variety of bacterial hosts. This review will focus on two processes indispensable for plasmid DNA maintenance. We will summarize recent understanding of the molecular mechanisms contributing to the RK2 DNA replication and partitioning.
Kolatka
Katarzyna
K
Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology, University of Gdansk, Kladki 24, Gdansk, Poland.
Kubik
Slawomir
S
Rajewska
Magdalena
M
Konieczny
Igor
I
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D003227:Conjugation, Genetic / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000502:physiology*; D011815:R Factors / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins