Interleukin-11 receptor alpha is expressed on canine osteosarcoma.
Osteosarcoma (OSA) is the most common bone tumour in humans and companion animals, and has a poor long-term prognosis. The identification of new markers and targeted therapies may help increase long-term survival of these patients. Previous studies have demonstrated that interleukin-11 receptor alpha (IL-11Ralpha) is expressed in human and murine OSA but not in normal bone. The current study demonstrated via western analysis, immunoflourescence and immunohistochemistry that IL-11Ralpha was expressed in primary canine OSA tissues as well as in a number of canine OSA cell lines, but not in normal canine bone. Cytotoxin-conjugated antibodies targeting IL-11Ralpha-mediated canine OSA cytotoxicity. Thus, canine OSA may be a valuable model for the evaluation of IL-11Ralpha directed therapies.
Sottnik
J L
JL
Department of Clinical Sciences, Animal Cancer Center, Colorado State University, Fort Collins, CO, USA.
Thamm
D H
DH
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D001859:Bone Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000662:veterinary*; D001842:Bone and Bones / Q000737:chemistry; D002460:Cell Line; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D015972:Gene Expression Regulation, Neoplastic; D053703:Interleukin-11 Receptor alpha Subunit / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation / Q000662:veterinary; D012516:Osteosarcoma / Q000737:chemistry / Q000378:metabolism / Q000662:veterinary*
D014408:Biomarkers, Tumor; D053703:Interleukin-11 Receptor alpha Subunit