[Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection].
In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen (HCAg) detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus (HCV) monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% (V/V) aminosilane solution and 3% (V/V) glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.
Xie
Li
L
Beijing You'an Hospital, Capital Medical University, Beijing 100069, China. XL811CN@163.com
Guan
Yueping
Y
Ge
Ying
Y
Shi
Hongbo
H
D055910:Antibodies, Immobilized / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000737:chemistry / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000032:analysis / Q000276:immunology*; D006801:Humans; D012822:Silicon Dioxide / Q000737:chemistry*
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D012822:Silicon Dioxide