Electron emission and product analysis of estrone: progesterone interactions studied by experiments in vitro.
Recent studies showed that hormones like progesterone, testosterone, etc. can eject [Formula: see text] (solvated electrons). By means of electron transfer processes via the brain, the hormones communicate with other biological systems in the organism. The present study proves that also estrone is able to emit electrons. Their yield strongly depends on the concentration of the hormone, temperature and on the absorbed energy. The metabolites resulting from this process are likewise able to generate electrons, however with much smaller yields. The formation of the estrone metabolites is studied by HPLC-analyses. In vitro experiments with MCF-7 cells demonstrate the distinct effect of progesterone on the carcinogenity of estrone metabolites. Probable reaction mechanisms for explanation of the observed effects are postulated.
Gerschpacher
Marion
M
Department of Gynecologic Endocrinology and Reproductive Medicine, University Hospital of Vienna, Vienna, Austria.
Getoff
Nikola
N
Hartmann
Johannes
J
Schittl
Heike
H
Danielova
Iren
I
Ying
Shaobin
S
Huber
Johannes C
JC
Quint
Ruth M
RM
D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004583:Electrons; D004958:Estradiol / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D004970:Estrone / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D011374:Progesterone / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology
D004970:Estrone; D011374:Progesterone; D004958:Estradiol