Cold tolerance of the overwintering larval instars of light brown apple moth Epiphyas postvittana.
The light brown apple moth, Epiphyas postvittana, a leafroller native to southeastern Australia was discovered in California in 2006. The highly polyphagous nature of this pest adds to the importance of being able to predict the potential distribution of this invader across the North American continent. The spread of ectothermic species that lack winter diapause, such as E. postvittana, can be limited by their ability to tolerate cold temperature extremes. In this study we examined the cold hardiness of 4th to 6th instar E. postvittana, the only life stages known to overwinter in California, through a combination of supercooling point (SCP) and mortality at low temperatures. Our results showed that the mean SCP for E. postvittana ranged from -14.1 degrees C for 6th instars to -16.0 degrees C for 4th instars. Lethal time leading to 50% mortality (LT(50)) for the three instars combined were 2.5 h at -10.5 degrees C, 41 h at -6.5 degrees C and 198 h at -0.9 degrees C. At 3 degrees C, the LT(50) of 4th instars was significantly lower at 775 h than that for 5th and 6th instars combined at 1029 h. The cold hardiness characteristics of later-instar E. postvittana larvae were comparable to those of pink bollworm, Pectinophora gossypiella, a diapausing invasive with a geographic distribution restricted to southern California. Slightly greater cold hardiness is shown by the indigenous non-diapausing leafroller Argyrotaenia franciscana, which is restricted to the Pacific Coast of North America. We therefore conclude that the moderate cold hardiness of E. postvittana will substantially limit its spread into northern temperate regions of North America.
Bürgi
Linda P
LP
Department of Environmental Science, Policy and Management, University of California Berkeley, Berkeley, CA 94720-3114, USA. lpbuergi@berkeley.edu
Mills
Nick J
NJ
D000818:Animals; D003080:Cold Temperature; D006605:Hibernation / Q000502:physiology*; D008675:Metamorphosis, Biological; D009036:Moths / Q000502:physiology*; D012621:Seasons