Influence of alcohol treatments on the activity of lipases immobilized on methyl-modified silica aerogels.
The effects of alcohol treatment on the activity and loading amount of Candida rugosa lipase (CRL), Candida Antarctica lipase B (CALB) and Porcine Pancreas lipase (PPL) immobilized on methyl-modified silica aerogels were investigated, and the fluorescent analysis was used to explore the change of lipase hydrophobicity in aqueous solution caused by alcohols. It is found that alcohol types and the stages at which alcohol was added significantly influenced the performance of immobilized lipases through changing the hydrophobicity of the molecules. For CRL and PPL, five kinds of alcohol were added in the adsorption process, and n-butanol and isopropanol improved the apparent activity of CRL and PPL up to 2.5 times and 2 times those of the untreated ones, respectively; however, for CALB, it is better to activate the immobilized CALB after the adsorption process, and the apparent activity of CALB increased up to 2.76 times through n-butanol treatment.
Gao
Siliang
S
State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, China.
Wang
Wenwen
W
Wang
Yujun
Y
Luo
Guangsheng
G
Dai
Youyuan
Y
D000438:Alcohols / Q000494:pharmacology*; D000818:Animals; D002175:Candida / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005782:Gels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000378:metabolism*; D008745:Methylation / Q000187:drug effects; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions; D012997:Solvents / Q000494:pharmacology; D034421:Sus scrofa; D013696:Temperature; D013997:Time Factors
D000438:Alcohols; D004800:Enzymes, Immobilized; D005782:Gels; D012996:Solutions; D012997:Solvents; D012822:Silicon Dioxide; D008049:Lipase