title: Ethanol extract of Lycoris radiata induces cell death in B16F10 melanoma via p38-mediated AP-1 activation.
abstract: Some active alkaloids isolated from Lycoris, a bulbous perennial herb, was shown to possess various anti-tumor and anti-inflammatory activities. In this study, we evaluated the in vitro apoptotic effect of ethanol extract from Lycoris radiata (LRE) and further probed the underlying molecular mechanisms of LRE effects. The survival rate of B16F10 melanoma cells exposed to LRE was decreased in a dose-dependent manner, cell growth was retarded by arresting cell cycle at G1 phase and apoptotic appearance such as caspase-3 activation as well as DNA fragmentation was observed by LRE treatment. In addition, LRE induced p38 and c-Jun phosphorylation, followed by activation of transcription factor AP-1. Pretreatment with the p38 inhibitor (SB203580) blocked LRE-induced AP-1 transcriptional activity, and curcumin, AP-1 inhibitor, dramatically inhibited LRE-induced apoptosis in B16F10 melanoma cells. Our results collectively indicate that LRE-mediated apoptosis occurs through the activation of p38 and AP-1 pathway and potentially LRE exhibits anti-cancer activity against B16F10 melanoma cells.
authors.affiliation: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Minsik
authors.initials: M
authors.lastname: Son
authors.forename: Aeyung
authors.initials: A
authors.lastname: Kim
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Lee
authors.forename: Chul-Hong
authors.initials: CH
authors.lastname: Park
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Heo
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology; D031426:Lycoris / Q000737:chemistry; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism* / Q000502:physiology
chemical_list: D010936:Plant Extracts; D018808:Transcription Factor AP-1; D000431:Ethanol; D048051:p38 Mitogen-Activated Protein Kinases