[Determination of tomatine in Solanum cathayanum by RP-HPLC].
OBJECTIVE
To develop an HPLC method for determination of tomatine in the dried Solanum cathayanum of China Hubei Enshi.


METHODS
The analysis was performed on a YMC-Pack ODS-AA column (4. 6 mm x 150 mm, 5 microm) eluted with acetonitrile and water in gradient mode. The concentration of acetonitrile in the mobile phase changes from 20% to 100% within 60 minutes. The detection wavelength was set 203 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.


RESULTS
The linear relationship of tomatine was determined within the range from 0.1-0.6 g x L(-1) (r = 0.999 7). The average recovery as 98.93% with RSD 1.2%.


CONCLUSIONS
A convenient and reliable method was developed to determine the content of tomatine in the dried S. cathayanum.
Guo
Jianghong
J
Hubei Key Laboratory of Natural Products Research and Development, China Three Gorges University, Yichang 443002, China.
Zhou
Yuan
Y
Liu
Rong
R
Xie
Mingxia
M
Zou
Kun
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D032322:Solanum / Q000737:chemistry; D014053:Tomatine / Q000032:analysis*
D014053:Tomatine