Ghrelin improves disturbed myocardial energy metabolism in rats with heart failure induced by isoproterenol.
To explore the effects of ghrelin on disturbed myocardial energy metabolism during chronic heart failure (CHF). Rats were subcutaneously injected with isoproterenol (ISO) for 10 days with or without ghrelin for another 10 days. Enzyme immunoassay was to measure ghrelin concentrations. Compared with the control group, ISO-treated rats showed suppressed cardiac function with high ghrelin/GHS-R expressions. These rats also showed the decreases in food consumption and weight. The decreased levels of plasma glucose and myocardial glucogen, but the high lactate in blood and myocardium showed myocardial metabolic disturbance. Compared with the group given ISO alone, the rats with ghrelin (20 and 100 microg/kg/day) improved cardiac dysfunction and increased food intake by 13.5 and 14.2% (both P < 0.01), and rate of weight gain by 95% (P < 0.05) and 1.71-fold (P < 0.01), respectively. The plasma glucose were increased by 49.7 and 50.8% (both P < 0.01), and myocardial glucogen, by 40.5 and 51.7% (both P < 0.01), but blood lactate decreased by 1.56- and 1.96-fold (both P < 0.01), and myocardial lactate by 32.1 and 48.7% (both P < 0.05), respectively. Their MCT1 mRNA and protein expressions increased. The myocardial ghrelin/GHS-R pathway can be upregulated during CHF. The ghrelin can attenuate cardiac dysfunction and energy metabolic disturbance in CHF rats.
Xu
Jian-Ping
JP
Department of Physiology and Pathophysiology, Capital Medical University, Beijing 100069, China.
Wang
Hong-Xia
HX
Wang
Wen
W
Zhang
Li-Ke
LK
Tang
Chao-Shu
CS
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004452:Echocardiography; D004734:Energy Metabolism / Q000187:drug effects*; D054439:Ghrelin / Q000494:pharmacology*; D006333:Heart Failure / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007545:Isoproterenol / Q000633:toxicity*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
D001786:Blood Glucose; D054439:Ghrelin; D007545:Isoproterenol; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1