title: Colored potato extracts induce superoxide dismutase-2 mRNA via ERK1/2 pathway in HepG2 cells.
abstract: Rats fed a diet containing Shadow Queen (SQ), an anthocyanin-rich potato cultivar, previously showed an increase in the hepatic superoxide dismutase (SOD)-2 mRNA level. We investigated whether an extract of SQ would directly increase the hepatic SOD-2 mRNA level in HepG2 cells. Furthermore, we estimated the intracellular signaling pathway for the induction of SOD-2 mRNA expression. HepG2 cells were stimulated using extracts of four crops, including SQ, for 12 h; only extracts of colored potatoes induced SOD-2 mRNA expression significantly. This induction of SOD-2 mRNA expression was blocked by an inhibitor of the extracellular signal-related kinase (ERK) 1/2 pathway. Furthermore, an extract of SQ increased the phosphorylation of ERK1/2 after 15 or 30 min of stimulation. These data indicate that an extract of SQ directly induces hepatic SOD-2 mRNA expression via activation of ERK1/2 pathway in HepG2 cells.
authors.affiliation: Memuro Upland Farming Research Station, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido 082-0081, Japan. hasshy@affrc.go.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Noda
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamauchi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Takigawa
authors.forename: Chie
authors.initials: C
authors.lastname: Matsuura-Endo
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Han
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fukushima
mesh_terms: D001947:Breeding; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D010766:Phosphorylation; D028321:Plant Preparations / Q000494:pharmacology*; D035281:Plant Tubers; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects; D011198:Solanum tuberosum / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D000099156:Superoxide Dismutase 2
chemical_list: D019950:Mitogen-Activated Protein Kinase 1; D028321:Plant Preparations; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2