title: Chlorination by-products of fenitrothion.
abstract: The mutagens produced through chemical reaction between chlorine and the insecticide fenitrothion were studied by using a quadrupole GC-MS. The mutagenicity and the mutagen formation potential (MFP) of the identified by-products were evaluated by the Ames assay (preincubation method) using Salmonella typhimurium TA100 without exogenous activation by S9 mix (TA100-S9). Before conducting GC/MS analyses, six compounds were presumed to be produced in chlorinated fenitrothion. These compounds were confirmed to be produced by the GC/MS analyses, but none of them were mutagenic. One of the chlorination by-products, 3-methyl-4-nitrophenol, has 19 times greater MFP than that of fenitrothion. This result suggests that a major mutagen in chlorinated fenitrothion will be produced via a chemical reaction between chlorine and 3-methyl-4-nitrophenol.
authors.affiliation: Department of Bioengineering, Kagoshima University, Kagoshima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kishida
authors.forename: T
authors.initials: T
authors.lastname: Kumabe
authors.forename: H
authors.initials: H
authors.lastname: Takanashi
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: A
authors.initials: A
authors.lastname: Ohki
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: T
authors.initials: T
authors.lastname: Kameya
mesh_terms: D005278:Fenitrothion / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D054879:Halogenation; D007306:Insecticides / Q000737:chemistry*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry*
chemical_list: D007306:Insecticides; D009153:Mutagens; D005278:Fenitrothion