title: Cerebralcare Granule, a Chinese herb compound preparation, improves cerebral microcirculatory disorder and hippocampal CA1 neuron injury in gerbils after ischemia-reperfusion.
abstract: OBJECTIVE
  Cerebralcare Granule (CG) is a Chinese herb compound preparation that has been used for treatment of cerebrovascular related diseases. However, the effect of post-treatment with CG on ischemia and reperfusion (I/R) induced cerebral injury is so far unclear.
  
  
  METHODS
  In present study, cerebral global I/R was induced in Mongolian gerbils by clamping bilateral carotid arteries for 30 min followed by reperfusion for 5 days, and CG (0.4 g/kg or 0.8 g/kg) was administrated 3h after the initiation of reperfusion.
  
  
  RESULTS
  Post-treatment with CG for 5 days attenuated the I/R-induced production of hydrogen peroxide in, leukocyte adhesion to, and albumin leakage from cerebral microvessels, and, meanwhile, protected neuron from death, reduced the number of caspase-3- and Bax-positive cells, and increased Bcl-2-positive cells in hippocampal CA1 region.
  
  
  CONCLUSIONS
  The results suggest that CG given after initiation of reperfusion is able to ameliorate cerebral microvascular dysfunction and hippocampal CA1 neuron damage caused by I/R.
authors.affiliation: Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
authors.forename: Chang-Man
authors.initials: CM
authors.lastname: Zhou
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Bai-He
authors.initials: BH
authors.lastname: Hu
authors.forename: Xin-Yong
authors.initials: XY
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Chang
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: An
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Fan
authors.forename: Chuan-She
authors.initials: CS
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002199:Capillary Permeability; D002317:Cardiovascular Agents / Q000008:administration & dosage / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002550:Cerebral Veins / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005849:Gerbillinae; D006861:Hydrogen Peroxide / Q000378:metabolism; D036904:Leukocyte Rolling / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D012709:Serum Albumin / Q000378:metabolism; D014699:Venules / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D012709:Serum Albumin; D051028:bcl-2-Associated X Protein; C553222:cerebralcare preparation; D006861:Hydrogen Peroxide; D053148:Caspase 3