Sex pheromone of the Spanish population of the beet armyworm Spodoptera exigua.
The pheromone composition of the Spanish population of the beet armyworm (BAW), Spodoptera exigua (Lepidoptera: Noctuidae), was identified. Analysis of female gland extracts showed the presence of compounds Z9,E12-14:Ac (1), Z9-14:Ac (2), Z11-16:Ac (3), Z9,E12-14:OH (4), Z9-14:OH (5), and Z11-16:OH (6) in a ratio of 26:11:1:22:31:9. The amount of compound per gland ranged from 2.08 ng for 5 to 0.09 ng for 3. However, analysis of female volatiles by SPME revealed only the presence of compounds, 1, 2, 3, and 5 in a 34:40:4:22 ratio. In electroantennogram assays, compound 1 elicited the highest response, and the C14 acetates evoked higher electrophysiological responses than the corresponding alcohols or C16 isomers. In a wind tunnel, no behavioral difference was observed between formulations based on the gland extracts and female volatiles. In both cases, males responded as when virgin females were used as the attractant source. Compound 1 alone elicited upwind flight by males, but required the presence of compound 5 in a 80:20 to 40:60 ratio for full activity. Ternary mixtures of 1, 5 and the minor components did not improve the performance of the 1+5 blend in a 60:40 ratio. In the field, the mixture 1+5+3 in a 56:37:7 ratio was the most attractive formulation, and is expected to be useful in future pest control strategies.
Acín
Patricia
P
Department of Biological Chemistry and Molecular Modelling, IQAC-CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.
Rosell
Gloria
G
Guerrero
Angel
A
Quero
Carmen
C
D000818:Animals; D002849:Chromatography, Gas; D005260:Female; D008297:Male; D012724:Sex Attractants / Q000737:chemistry*; D012726:Sexual Behavior, Animal; D018411:Spodoptera / Q000502:physiology*; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D012724:Sex Attractants; D055549:Volatile Organic Compounds