Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses.
All 16 subtypes of avian influenza viruses of low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four 2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species: goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA) titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity of replicating LPAIV and LPAIV antigens decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer. Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that goose and guinea pig erythrocytes are the best in HA assay for all subtypes of influenza viruses.
Wiriyarat
Witthawat
W
Faculty of Veterinary Science, Mahidol University, Nakhonpathom 73170, Thailand.
Lerdsamran
Hatairat
H
Pooruk
Phisanu
P
Webster
Robert G
RG
Louisirirotchanakul
Suda
S
Ratanakorn
Parntep
P
Chaichoune
Kridsada
K
Nateerom
Kannika
K
Puthavathana
Pilaipan
P
D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology / Q000821:virology*; D005777:Geese; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000378:metabolism; D006736:Horses; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology / Q000378:metabolism; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000821:virology*
D006388:Hemagglutinins