title: Comparative studies on the extraction of metagenomic DNA from the saline habitats of Coastal Gujarat and Sambhar Lake, Rajasthan (India) in prospect of molecular diversity and search for novel biocatalysts.
abstract: Extraction of total DNA from a given habitat assumes significance in metagenomics, due to the requirement of inhibitor free and high quality metagenome in good quantity for applications in molecular biology. DNA extraction and its quality assessment for PCR applications from saline soils of Coastal Gujarat and Sambhar Soda Lake, Rajasthan in India is described in a comparative manner. The mechanical and soft lysis methods were simple and efficient for rapid isolation of PCR amplifiable total genomic DNA. The results are significant as only few extreme environments, particularly saline habitats are explored for their metagenomic potential.
authors.affiliation: Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Siddhapura
authors.forename: S
authors.initials: S
authors.lastname: Vanparia
authors.forename: M K
authors.initials: MK
authors.lastname: Purohit
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D055162:Biocatalysis; D044822:Biodiversity; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D004587:Electrophoresis, Agar Gel; D010450:Endopeptidases / Q000235:genetics; D005618:Fresh Water; D007194:India; D056186:Metagenomics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D004247:DNA; D010450:Endopeptidases; C087822:alkaline protease