title: Bluetongue virus RNA detection by RT-qPCR in blood samples of sheep vaccinated with a commercially available inactivated BTV-8 vaccine.
abstract: In 2008, a country-wide bluetongue virus 8 (BTV-8) vaccination campaign has been initiated in Austria, using a single commercial inactivated BTV-8 vaccine. Based on preliminary data, we hypothesised that vaccine-derived BTV RNA is transiently detectable by reverse transcription quantitative real-time PCR (RT-qPCR) in the blood of vaccinated animals. Thus BTV-8 vaccine was administered to five BTV-naïve adult sheep and blood samples were taken at various time-points post-vaccination. BTV RNA was detectable by several RT-qPCR methods in all five animals, mainly within the first 9 days post-vaccination. These results show that RT-qPCR based testing for BTV-infection may be influenced by vaccination with certain inactivated BTV-8 vaccines.
authors.affiliation: Austrian Agency for Health and Food Safety (AGES), Institute for Veterinary Disease Control Moedling, A-2340 Moedling, Austria. adolf.steinrigl@ages.at
authors.forename: Adolf
authors.initials: A
authors.lastname: Steinrigl
authors.forename: Sandra
authors.initials: S
authors.lastname: Revilla-Fernández
authors.forename: Michaela
authors.initials: M
authors.lastname: Eichinger
authors.forename: Josef
authors.initials: J
authors.lastname: Koefer
authors.forename: Petra
authors.initials: P
authors.lastname: Winter
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000276:immunology / Q000517:prevention & control*; D001820:Bluetongue virus / Q000302:isolation & purification*; D005260:Female; D008297:Male; D010865:Pilot Projects; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep / Q000276:immunology* / Q000821:virology; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D012367:RNA, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines