Crocin attenuates cisplatin-induced renal oxidative stress in rats.
Reactive oxygen species (ROS) and oxidative damage are the most important factors in cisplatin-induced acute renal failure. This study examined the protective effects of crocin against cisplatin-induced renal oxidative stress in rat. Animals were divided into five groups (n=6). Group 1 received normal saline (2 ml/day, i.p.). Group 2 received a single dose of cisplatin (5mg/kg, i.p.). Groups 3-5 received crocin (100, 200, and 400mg/kg, i.p., respectively) for four consecutive days beginning 1-h before a single dose of cisplatin (5mg/kg) on day 1. On day 5, blood samples were drawn and kidneys were removed for histopathological, biochemical and RT-PCR examinations. Twenty four hours urinary chemistries were measured. Blood urea and creatinine and urinary glucose and protein concentrations in crocin-treated groups were significantly lower compared to the cisplatin-treated group. Histopathological studies showed massive damage in the S(3) segment of proximal tubules in cisplatin-treated group but not in crocin-treated groups. Crocin treatment resulted in a significant reduction in malondialdehyde (MDA) concentration and produced a significant elevation in total thiol and glutathione peroxidase concentrations. There was a significant elevation in the mRNA expression of glutathione peroxidase in crocin-treated groups. The results suggest that crocin attenuates cisplatin-induced renal oxidative stress in rats.
Naghizadeh
Bahareh
B
Department of Pharmacology, Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran.
Mansouri
Seyed Mohammad Taghi
SM
Mashhadian
Nasser Vahdati
NV
D000818:Animals; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002338:Carotenoids / Q000494:pharmacology*; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D006029:Glycosuria / Q000139:chemically induced / Q000517:prevention & control; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011507:Proteinuria / Q000139:chemically induced / Q000517:prevention & control; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000970:Antineoplastic Agents; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002338:Carotenoids; D008315:Malondialdehyde; D012313:RNA; C029036:crocin; D003404:Creatinine; D005979:Glutathione Peroxidase; D002945:Cisplatin