Organ-related cigarette smoke-induced oxidative stress is strain-dependent.
BACKGROUND
Cigarette smoke (CS) is associated with oxidative stress in several organs because it contains high concentrations of free radicals and reactive oxygen species. Experimental models, using different strains, provide important insights into the genetic basis of diseases. This study sought to identify, in different mouse strains, the organ that is most-susceptible to CS-induced oxidative stress to obtain an optimized experimental animal model of oxidative injury induced by CS.


METHODS
Male Swiss, DBA/2, C3H, BALB/c, and C57BL/6 mice were exposed to CS 3 times a day (4 cigarettes per session) for 60 consecutive days. Control groups from the same strains were sham-treated. Protein content, malondialdehyde level, myeloperoxidase activity, and nitrite level were assayed in lung, liver, kidney, and brain from all strains. Catalase and glutathione peroxidase activities were measured. Analyses of data were done by using a 1-way ANOVA with Bonferroni's post-test (P<.05).


RESULTS
Cigarette smoke exposure resulted in distinct, organ-specific responses among strains. The survival rate of DBA/2 mice was lowest. BALB/c and C57BL/6 strains were more-susceptible to oxidative damage in the lung and liver. C3H and C57BL/6 mice were more-susceptible to oxidative damage in the brain. No renal oxidative damage was seen.


CONCLUSIONS
Mouse strains and individual organs display a range of susceptibilities to CS-induced oxidative stress. BALB/c and C57BL/6 strains appear to be the best choices as experimental models for studying CS effects on liver and lung, and C3H and C57BL/6 strains for CS-effects on the brain.
Rueff-Barroso
Carlos Romualdo
CR
Laboratory of Tissue Repair, Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Trajano
Eduardo Tavares Lima
ET
Alves
Jackson Nogueira
JN
Paiva
Rojane Oliveira
RO
Lanzetti
Manuella
M
Pires
Karla Maria Pereira
KM
Bezerra
Frank Silva
FS
Pinho
Ricardo Aurino
RA
Valenca
Samuel Santos
SS
Porto
Luis Cristóvão
LC
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D012907:Smoking / Q000009:adverse effects*
D002374:Catalase; D005979:Glutathione Peroxidase