A serum fucose-binding lectin (DlFBL) from adult Dicentrarchus labrax is expressed in larva and juvenile tissues and contained in eggs.
The purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of Dicentrarchus labrax (DlFBL) have been previously reported. We now describe the distribution and expression of DlFBL during fish ontogeny. Immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days post-hatching larvae to juveniles). Another fucose-binding lectin, similar to DlFBL in biochemical, immunochemical and agglutinating properties, was extracted and purified from eggs and appeared to be localized in the embryo yolk sack residual. DlFBL was found in columnar and goblet cells of the intestinal epithelium of larvae (from 20 days post-hatching) and juveniles and in parenchymal tissue of juveniles. DlFBL mRNA and protein were detected in the intestinal epithelium and in hepatocytes. An amplification product from degenerate primers indicates that lectin isotypes with DlFBL epitopes are expressed in eggs and embryos. Whether the lectin fraction isolated from eggs and embryos includes DlFBL of maternal origin remains unclear.
Parisi
M Giovanna
MG
Laboratory of Marine Immunobiology, Department of Animal Biology, University of Palermo, Via Archirafi 18, 90123, Palermo, Italy.
Cammarata
Matteo
M
Benenati
Gigliola
G
Salerno
Giuseppina
G
Mangano
Valentina
V
Vizzini
Aiti
A
Parrinello
Nicolò
N
D000818:Animals; D001492:Bass / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029941:Fish Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007814:Larva / Q000378:metabolism; D037102:Lectins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D010063:Ovum / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D029941:Fish Proteins; D037102:Lectins; D012333:RNA, Messenger; C027112:fucose-binding lectin