The O-antigen of Salmonella enterica O13 and its relation to the O-antigen of Escherichia coli O127.
The following structure of the O-polysaccharide (O-antigen) of Salmonella enterica O13 was established by chemical analyses along with 2D (1)H and (13)C NMR spectroscopy: -->2)-alpha-l-Fucp-(1-->2)-beta-d-Galp-(1-->3)-alpha-d-GalpNAc-(1-->3)-alpha-d-GlcpNAc-(1--> The O-antigen of S. enterica O13 was found to be closely related to that of Escherichia coli O127, which differs only in the presence of a GalNAc residue in place of the GlcNAc residue and O-acetylation. The location of the O-acetyl groups in the E. coli O127 polysaccharide was determined. The structures of the O-polysaccharides studied are in agreement with the DNA sequence of the O-antigen gene clusters of S. enterica O13 and E. coli O127 reported earlier.
Perepelov
Andrei V
AV
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
Liu
Bin
B
Senchenkova
Sof'ya N
SN
Shevelev
Sergei D
SD
Feng
Lu
L
Shashkov
Alexander S
AS
Wang
Lei
L
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011522:Protons; D019779:Salmonella enterica / Q000737:chemistry*
D002247:Carbon Isotopes; D019081:O Antigens; D011522:Protons