Free radical peroxidation products in cerebrospinal fluid and serum of patients with multiple sclerosis after glucocorticoid therapy.
Multiple sclerosis (MS) patients were found to have elevated thiobarbituric acid reactive material levels, increased soluble sulfhydryl groups and reduced protein sulfhydryl groups in cerebrospinal fluid and serum, and slightly reduced superoxide dismutase in serum, which suggested disease activating free radical peroxidation. Moreover, levels of these varied across methylprednisolone (MP) therapy. We observed significant differences in the levels of peroxidation products between MS patients and controls. These changes were most evident in relapse. After MP therapy, levels of these indicators approached control values, especially in the remission period. Our findings suggest that MP protects against free radical attack.
Mitosek-Szewczyk
Krystyna
K
Department of Neurology, Medical University in Lublin, Lublin, Poland. krystyna.mitosek@am.lublin.pl
Gordon-Krajcer
Wanda
W
Walendzik
Piotr
P
Stelmasiak
Zbigniew
Z
D000328:Adult; D005260:Female; D005609:Free Radicals / Q000097:blood* / Q000134:cerebrospinal fluid*; D005938:Glucocorticoids / Q000627:therapeutic use*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism
D005609:Free Radicals; D005938:Glucocorticoids; C000606290:SOD1 protein, human; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D008775:Methylprednisolone