Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.
Metazoan development involves a myriad of dynamic cellular processes that require cytoskeletal function. Nonmuscle myosin II plays essential roles in embryonic development; however, knowledge of its role in post-embryonic development, even in model organisms such as Drosophila melanogaster, is only recently being revealed. In this study, truncation alleles were generated and enable the conditional perturbation, in a graded fashion, of nonmuscle myosin II function. During wing development they demonstrate novel roles for nonmuscle myosin II, including in adhesion between the dorsal and ventral wing epithelial sheets; in the formation of a single actin-based wing hair from the distal vertex of each cell; in forming unbranched wing hairs; and in the correct positioning of veins and crossveins. Many of these phenotypes overlap with those observed when clonal mosaic analysis was performed in the wing using loss of function alleles. Additional requirements for nonmuscle myosin II are in the correct formation of other actin-based cellular protrusions (microchaetae and macrochaetae). We confirm and extend genetic interaction studies to show that nonmuscle myosin II and an unconventional myosin, encoded by crinkled (ck/MyoVIIA), act antagonistically in multiple processes necessary for wing development. Lastly, we demonstrate that truncation alleles can perturb nonmuscle myosin II function via two distinct mechanisms--by titrating light chains away from endogenous heavy chains or by recruiting endogenous heavy chains into intracellular aggregates. By allowing myosin II function to be perturbed in a controlled manner, these novel tools enable the elucidation of post-embryonic roles for nonmuscle myosin II during targeted stages of fly development.
Franke
Josef D
JD
Department of Biology, Duke University, Durham, NC 27708, USA.
Montague
Ruth A
RA
Kiehart
Daniel P
DP
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D049109:Cell Proliferation; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009024:Morphogenesis; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D014921:Wings, Animal / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development*
D024462:Myosin Type II