title: Identification of Ige-binding proteins of raw and cooked extracts of Loligo edulis (white squid).
abstract: Allergy to different classes of mollusks, including squid, which are members of the class Cephalopods has been reported. Tropomyosin, a major muscle protein, is the only well-recognized allergen in squid. The aim of this study was to characterize IgE-binding proteins of local Loligo edulis (white squid) consumed in Malaysia. Protein profiles and IgE-binding proteins were detected by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE) and immunoblotting using sera from 23 patients with positive skin prick test to raw squid extract. SDS-PAGE of the raw extract exhibited 21 protein bands (10-170 kDa) but those ranging from 19 to 29 kDa and 41 to 94 kDa were not found in the cooked extract. Immunoblotting of raw extract demonstrated 16 IgE-binding bands, ranging from 13 to 170 kDa. A heat-resistant 36 kDa protein, corresponding to squid tropomyosin, was identified as the major allergen of both extracts. In addition, a 50 kDa heat-sensitive protein was shown to be a major allergen of the raw extract. Our findings indicate that the allergen extract used for diagnosis of squid allergy should contain both the 36 kDa and 50 kDa proteins.
authors.affiliation: Allergy and Immunology Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. zailatul@imr.gov.my
authors.forename: Zailatul Hani Mohamad
authors.initials: ZH
authors.lastname: Yadzir
authors.forename: Rosmilah
authors.initials: R
authors.lastname: Misnan
authors.forename: Noormalin
authors.initials: N
authors.lastname: Abdullah
authors.forename: Faizal
authors.initials: F
authors.lastname: Bakhtiar
authors.forename: Masita
authors.initials: M
authors.lastname: Arip
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Murad
mesh_terms: D000485:Allergens / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D003296:Cooking; D004591:Electrophoresis, Polyacrylamide Gel; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D037502:Galectin 3 / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D049834:Loligo / Q000276:immunology*; D008296:Malaysia; D012758:Shellfish; D012882:Skin Tests
chemical_list: D000485:Allergens; D037502:Galectin 3