Antibacterial activity and biocompatibility of a chitosan-gamma-poly(glutamic acid) polyelectrolyte complex hydrogel.
In this study, we prepared a polyelectrolyte complex (PEC) hydrogel comprising chitosan as the cationic polyelectrolyte and gamma-poly(glutamic acid) (gamma-PGA) as the anionic polyelectrolyte. Fourier transform infrared spectroscopy revealed that ionic complex interactions existed in the chitosan-gamma-PGA PEC hydrogels. The compressive modulus increased upon increasing the degree of complex formation in the chitosan-gamma-PGA PEC hydrogel; the water uptake decreased upon increasing the degree of complex formation. At the same degree of complex formation, the compressive modulus was larger for the chitosan-dominated PEC hydrogels; the water uptake was larger for the gamma-PGA-dominated ones. Scanning electron microscopy images revealed the existence of interconnected porous structures (pore size: 30-100mum) in all of the chitosan-gamma-PGA PEC hydrogels. The chitosan-gamma-PGA PEC hydrogels also exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. In addition, in vitro cell culturing of 3T3 fibroblasts revealed that all the chitosan-gamma-PGA PEC hydrogels were effective in promoting cell proliferation, especially the positively charged ones (chitosan-dominated). Therefore, the chitosan-gamma-PGA polyelectrolyte hydrogel appears to have potential as a new material for biomedical applications.
Tsao
Ching Ting
CT
Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei City 10617, Taiwan.
Chang
Chih Hao
CH
Lin
Yu Yung
YY
Wu
Ming Fung
MF
Wang
Jaw-Lin
JL
Han
Jin Lin
JL
Hsieh
Kuo Huang
KH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004573:Electrolytes / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D010316:Particle Size; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004573:Electrolytes; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan