title: A low-cost, portable, and quantitative spectral imaging system for application to biological tissues.
abstract: The ability of diffuse reflectance spectroscopy to extract quantitative biological composition of tissues has been used to discern tissue types in both pre-clinical and clinical cancer studies. Typically, diffuse reflectance spectroscopy systems are designed for single-point measurements. Clinically, an imaging system would provide valuable spatial information on tissue composition. While it is feasible to build a multiplexed fiber-optic probe based spectral imaging system, these systems suffer from drawbacks with respect to cost and size. To address these we developed a compact and low cost system using a broadband light source with an 8-slot filter wheel for illumination and silicon photodiodes for detection. The spectral imaging system was tested on a set of tissue mimicking liquid phantoms which yielded an optical property extraction accuracy of 6.40 +/- 7.78% for the absorption coefficient (micro(a)) and 11.37 +/- 19.62% for the wavelength-averaged reduced scattering coefficient (micro(s)').
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. henry.fu@duke.edu
authors.forename: Henry L
authors.initials: HL
authors.lastname: Fu
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.forename: Justin Y
authors.initials: JY
authors.lastname: Lo
authors.forename: Greg M
authors.initials: GM
authors.lastname: Palmer
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Kuech
authors.forename: Nimmi
authors.initials: N
authors.lastname: Ramanujam
mesh_terms: D003198:Computer Simulation; D021621:Imaging, Three-Dimensional / Q000191:economics* / Q000379:methods*; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D013057:Spectrum Analysis / Q000191:economics* / Q000379:methods*