Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation.
We described a hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species. A nanopipette/nanoring electrode probe provided submicrometer resolution of the electrochemical measurement on surfaces with complex topology. The SECM/SICM probe had an aperture radius of 220 nm. The inner and outer radii of the SECM Au nanoring electrode were 330 and 550 nm, respectively. Characterization of the probe was performed with scanning electron microscopy (SEM), cyclic voltammetry (CV), and approach curve measurements. SECM/SICM was applied to simultaneous imaging of topography and electrochemical responses of enzymes (horse radish peroxidase (HRP) and glucose oxidase (GOD)) and single live cells (A6 cells, superior cervical ganglion (SCG) cells, and cardiac myocytes). The measurements revealed the distribution of activity of the enzyme spots on uneven surfaces with submicrometer resolution. SECM/SICM acquired high resolution topographic images of cells together with the map of electrochemical signals. This combined technique was also applied to the evaluation of the permeation property of electroactive species through cellular membranes.
Takahashi
Yasufumi
Y
Graduate School of Environmental Studies, Tohoku University, Aramaki Aoba 6-6-11-605, Sendai 980-8579, Japan.
Shevchuk
Andrew I
AI
Novak
Pavel
P
Murakami
Yumi
Y
Shiku
Hitoshi
H
Korchev
Yuri E
YE
Matsue
Tomokazu
T
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging; D040081:Protein Array Analysis
D005949:Glucose Oxidase; D006735:Horseradish Peroxidase