title: Protective immunity against H5N1 influenza virus by a single dose vaccination with virus-like particles.
abstract: We generated influenza virus-like particles (VLPs) containing the wild type (WT) H5 hemagglutinin (HA) from A/Viet Nam/1203/04 virus or a mutant H5 HA with a deletion of the multibasic cleavage motif. VLPs containing mutant H5 HA were found to be as immunogenic as VLPs containing WT HA. A single intramuscular vaccination with either type of H5 VLPs provided complete protection against lethal challenge. In contrast, the recombinant H5 HA vaccine was less immunogenic and vaccination even with a 5 fold higher dose did not induce protective immunity. VLP vaccines were superior to the recombinant HA in inducing T helper type 1 immune responses, hemagglutination inhibition titers, and antibody secreting cells, which significantly contribute to inducing protective immunity after a single dose vaccination. This study provides insights into the potential mechanisms of improved immunogenicity by H5 VLP vaccines as an approach to improve the protective efficacy against potential pandemic viruses.
authors.affiliation: Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, Atlanta, Georgia, USA.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Song
authors.forename: Jaber
authors.initials: J
authors.lastname: Hossain
authors.forename: Dae-Goon
authors.initials: DG
authors.lastname: Yoo
authors.forename: Aleksandr S
authors.initials: AS
authors.lastname: Lipatov
authors.forename: C Todd
authors.initials: CT
authors.lastname: Davis
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Chen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hogan
authors.forename: Ruben O
authors.initials: RO
authors.lastname: Donis
authors.forename: Richard W
authors.initials: RW
authors.lastname: Compans
authors.forename: Sang-Moo
authors.initials: SM
authors.lastname: Kang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D001402:B-Lymphocytes / Q000502:physiology; D002460:Cell Line; D004334:Drug Administration Schedule; D005260:Female; D006385:Hemagglutination Inhibition Tests; D007074:Immunoglobulin G / Q000097:blood; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D013154:Spleen / Q000166:cytology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007252:Influenza Vaccines