Production and purification of polyclonal antibodies.
Polyclonal antibodies are derived from multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against a single molecular species of antigen recognize multiple epitopes on a target molecule resulting in signal amplification in indirect immunoassays, including immuno-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
Nakazawa
Masami
M
Department of Applied Biological Chemistry, Osaka Prefecture University, Osaka, Japan.
Mukumoto
Mari
M
Miyatake
Kazutaka
K
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification*; D005260:Female; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D007106:Immune Sera