title: Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity.
abstract: BACKGROUND
  It is universally acknowledged that genome segment 4 of group A rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen VP4.
  
  
  RESULTS
  To determine which genome segment of three group A equine rotavirus strains (H-2, FI-14 and FI-23) with P[12] specificity encodes the VP4, we analyzed dsRNAs of strains H-2, FI-14 and FI-23 as well as their reassortants by polyacrylamide gel electrophoresis (PAGE) at varying concentrations of acrylamide. The relative position of the VP4 gene of the three equine P[12] strains varied (either genome segment 3 or 4) depending upon the concentration of acrylamide. The VP4 gene bearing P[3], P[4], P[6], P[7], P[8] or P[18] specificity did not exhibit this phenomenon when the PAGE running conditions were varied.
  
  
  CONCLUSIONS
  The concentration of acrylamide in a PAGE gel affected VP4 gene coding assignment of equine rotavirus strains bearing P[12] specificity.
authors.affiliation: Rotavirus Vaccine Development Section, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: LaShanda M
authors.initials: LM
authors.lastname: Long-Croal
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wen
authors.forename: Eileen N
authors.initials: EN
authors.lastname: Ostlund
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Hoshino
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D036022:Capsid Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012401:Rotavirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; D020106:Acrylamide