title: Development of biodegradable nanoparticles for delivery of quercetin.
abstract: The antioxidant molecule quercetin has been encapsulated on poly-D,L-lactide (PLA) nanoparticles by solvent evaporation method for the improvement of its poor aqueous solubility and stability. The surface morphology and average size of PLA and quercetin loaded PLA nanoparticles are 170+/-25 and 130+/-30 nm respectively. The antioxidant activities of the PLA encapsulated quercetin nanomedicine are identical to free quercetin. The nanoencapsulation efficiency of quercetin evaluated by HPLC and antioxidant assay is 96.7%. The in vitro release kinetics under physiological condition show initial burst release followed by slow and sustained release. The complete release and maximum retention of quercetin is 72 and 96h respectively. The less fluorescence quenching efficiency of quercetin-PLA nanoparticles than free quercetin on BSA confirms the controlled release of quercetin from PLA nanoparticles. These properties of PLA encapsulated quercetin molecule pave way for encapsulating various therapeutically less useful highly active antioxidant molecules towards the development of better therapeutic compounds.
authors.affiliation: Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, Palampur 176061, HP, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
authors.forename: Yogesh B
authors.initials: YB
authors.lastname: Pakade
authors.forename: Bikram
authors.initials: B
authors.lastname: Singh
authors.forename: Subhash Chandra
authors.initials: SC
authors.lastname: Yadav
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D004337:Drug Carriers; D011091:Polyesters; C033616:poly(lactide); D011794:Quercetin