Expression of rabbits BAFF in Escherichia coli with costimulatory effect on B cell proliferation.
In this paper, the cDNA encoding the extracellular soluble domain of rabbit BAFF (rsBAFF) was subcloned into the pET28a vector and the recombinant rsBAFF protein was solubly produced in Escherichia coli. Recombinant rsBAFF protein of 17.1kDa was then purified to a purity of 95% by using Ni(2+)-IDA resin. In vitro, purified rsBAFF protein was able to promote the survival/proliferation of B-lymphocytes of rabbits, mice and humans in the presence of anti-IgM antibodies. These findings indicate that the recombinant rsBAFF produced in E. coli have the bioactivity, and functional cross-reactivity occurs between rabbit and other mammalians BAFF proteins.
Zhang
Jia-Xin
JX
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, PR China.
Zhang
Wei
W
Zhang
Jie
J
Shui
Yan
Y
Chen
Su-Juan
SJ
Sang
Ming
M
Xu
Qiang
Q
Zhang
Shuang-Quan
SQ
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D053264:B-Cell Activating Factor / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D049109:Cell Proliferation / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D053264:B-Cell Activating Factor; C044203:anti-IgM