[Analysis of the role of various components of culture media during the proliferation of mouse neuroblastoma NIE-115 cells].
The values of the parameters of serum-free media (concentration of Na+, amino acids, and carbohydrates, as well as the pH values) have been determined at which the rate of the differentiation of neuroblastoma cells is minimal, and the rate of proliferation is maximal. It was shown that media inducing the differentiation of 70% of cells during the cell cycle provide the maximal time of survival of differentiated cells.
Aslanidi
K B
KB
Miakisheva
S N
SN
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D017209:Apoptosis; D002241:Carbohydrates / Q000737:chemistry; D002414:Cations, Monovalent; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009995:Osmosis; D012964:Sodium / Q000737:chemistry; D013997:Time Factors
D000596:Amino Acids; D002241:Carbohydrates; D002414:Cations, Monovalent; D003470:Culture Media; D016895:Culture Media, Serum-Free; D012964:Sodium