[Cytochemical and ultrastructural characteristic of BHK-21 cells exposed to dopamine].
BHK-21 cells were incubated in a medium containing dopamine (DA) and then their catecholamine content evaluated by using the Falck cytochemical method. The significant intensification of cell fluorescence as compared to that one in control preparations was detected; this effect was proportional to DA concentration and exposure duration and was more pronounced in cells in suspension than in those attached to the substrate. Simultaneous ultrastructural investigation has shown that an increased intensity of the cytoplasm fluorescence correlated with the appearance of the dense network of fibrils that were morphologically identified as F-actin microfilaments. Prior blockade of dopaminergic receptors by haloperidol did not change the following DA effect both on the fluorescence intensity and cell ultrastructure. The data obtained suggest that DA chronically acting on the living cells was able to penetrate into the cytoplasm, causing actin polymerization and incorporating into the newly formed actin cytoskeleton. Structurally, this may be manifested by cytoskeleton and its derivative hypertrophy, that could have a substantial effect on general morphology of the cell.
Shubina
V S
VS
Lavrovskaia
V P
VP
Bezgina
E N
EN
Pavlik
L L
LL
Moshkov
D A
DA
D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism* / Q000648:ultrastructure*; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology*; D015259:Dopamine Agents / Q000378:metabolism / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D011954:Receptors, Dopamine / Q000378:metabolism
D000199:Actins; D015259:Dopamine Agents; D018492:Dopamine Antagonists; D011954:Receptors, Dopamine; D006220:Haloperidol; D004298:Dopamine