title: A real-time quantitative assay for hepatitis B DNA virus (HBV) developed to detect all HBV genotypes.
abstract: Hepatitis B virus (HBV) is a major cause of chronic liver disease worldwide. Besides genotype, quantitative analysis of HBV infection is extensively used for monitoring disease progression and treatment. Affordable viral load monitoring is desirable in resource-limited settings and it has been already shown to be useful in developing countries for other viruses such as Hepatitis C virus (HCV) and HIV. In this paper, we describe the validation of a real-time PCR assay for HBV DNA quantification with TaqMan chemistry and MGB probes. Primers and probes were designed using an alignment of sequences from all HBV genotypes in order to equally amplify all of them. The assay is internally controlled and was standardized with an international HBV panel. Its efficacy was evaluated comparing the results with two other methods: Versant HBV DNA Assay 3.0 (bDNA, Siemens, NY, USA) and another real-time PCR from a reference laboratory. Intra-assay and inter-assay reproducibilities were determined and the mean of CV values obtained were 0.12 and 0.09, respectively. The assay was validated with a broad dynamic range and is efficient for amplifying all HBV genotypes, providing a good option to quantify HBV DNA as a routine procedure, with a cheap and reliable protocol.
authors.affiliation: Departamento de Patologia Clínica, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil. rositnik@hotmail.com
authors.forename: Roberta
authors.initials: R
authors.lastname: Sitnik
authors.forename: Angela
authors.initials: A
authors.lastname: Paes
authors.forename: Cristovão Pitangueira
authors.initials: CP
authors.lastname: Mangueira
authors.forename: João Renato Rebello
authors.initials: JR
authors.lastname: Pinho
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D017931:DNA Primers; D004279:DNA, Viral