Evolution of damage in the lens after in vivo close to threshold exposure to UV-B radiation: cytomorphological study of apoptosis.
The purpose of the present study was to investigate cataractogenesis and recovery of lens damage after in vivo close to threshold ultraviolet (UV)-B radiation around 300 nm. Eighty six-week-old albino Sprague-Dawley rats were familiarized to a rat restrainer five days prior to exposure. Groups of non-anesthetized rats were exposed unilaterally to 8 kJ/m(2) UVR-300 nm. The animals were sacrificed at 1, 7, 48 and 336h following exposure. The lenses were extracted for imaging of dark-field lens macro anatomy and measurement of intensity of forward lens light scattering to quantify lens opacities. Three exposed lenses and one non-exposed lens from each time interval were examined with light and transmission electron microscopy (TEM). Macro anatomy and lens light scattering revealed that all contralateral non-exposed lenses were clear. The degree of lens opacity (difference in lens light scattering between exposed and non-exposed lenses) increased during the 336h, reaching a plateau towards the end of the observation period. Light microscopy and TEM demonstrated that apoptotic features appeared in the epithelium already 1h after UVR exposure, and small vacuoles were seen in the outer cortex. Epithelial damage occurs during the first 48h after exposure and is followed by regenerative repair at 336h post-exposure. Apoptotic epithelial cells were phagocytized by adjacent epithelial cells. Cortical fiber cells exhibited increasing damage throughout the observation period without any clear repair after 336h. In conclusion, acute UVR-induced cataract is partly a reversible. Lens epithelium is a primary target for UVR exposure. Damage to cortical fiber cells remained irreversible.
Galichanin
Konstantin
K
St Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden. konstantin.galichanin@neuro.uu.se
Löfgren
Stefan
S
Bergmanson
Jan
J
Söderberg
Per
P
D000818:Animals; D017209:Apoptosis; D002386:Cataract / Q000209:etiology / Q000473:pathology*; D004847:Epithelial Cells / Q000528:radiation effects / Q000648:ultrastructure; D005260:Female; D007904:Lens Cortex, Crystalline / Q000528:radiation effects / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000503:physiopathology / Q000528:radiation effects* / Q000648:ultrastructure; D008027:Light; D011829:Radiation Dosage; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation; D012684:Sensory Thresholds; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014617:Vacuoles / Q000648:ultrastructure; D014945:Wound Healing