Predicting nucleosome positioning using a duration Hidden Markov Model.
BACKGROUND
The nucleosome is the fundamental packing unit of DNAs in eukaryotic cells. Its detailed positioning on the genome is closely related to chromosome functions. Increasing evidence has shown that genomic DNA sequence itself is highly predictive of nucleosome positioning genome-wide. Therefore a fast software tool for predicting nucleosome positioning can help understanding how a genome's nucleosome organization may facilitate genome function.


RESULTS
We present a duration Hidden Markov model for nucleosome positioning prediction by explicitly modeling the linker DNA length. The nucleosome and linker models trained from yeast data are re-scaled when making predictions for other species to adjust for differences in base composition. A software tool named NuPoP is developed in three formats for free download.


CONCLUSIONS
Simulation studies show that modeling the linker length distribution and utilizing a base composition re-scaling method both improve the prediction of nucleosome positioning regarding sensitivity and false discovery rate. NuPoP provides a user-friendly software tool for predicting the nucleosome occupancy and the most probable nucleosome positioning map for genomic sequences of any size. When compared with two existing methods, NuPoP shows improved performance in sensitivity.
Xi
Liqun
L
Department of Statistics, Northwestern University, Evanston, IL 60208, USA.
Fondufe-Mittendorf
Yvonne
Y
Xia
Lei
L
Flatow
Jared
J
Widom
Jonathan
J
Wang
Ji-Ping
JP
D004247:DNA / Q000235:genetics; D016678:Genome; D023281:Genomics / Q000379:methods*; D008390:Markov Chains; D009707:Nucleosomes / Q000737:chemistry* / Q000648:ultrastructure; D010363:Pattern Recognition, Automated / Q000379:methods; D012984:Software
D009707:Nucleosomes; D004247:DNA