title: Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines.
abstract: The present study is aimed to investigate the effects of Chenopodium album (leaves) on the growth of estrogen dependent (MCF-7) and estrogen independent (MDA-MB-468) human breast cancer cell lines. The different solvent extracts (petroleum ether, ethyl acetate and methanol) were assessed for their cytotoxicity using TBE (Trypan blue exclusion) and MTT [3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium] bioassay. These cells were cultured in MEM (minimum essential medium) medium and incubated with the dilution series of extracts (10-100 mg/ml) in CO(2) incubator at 37 degrees C for 24 h. Among the various extracts studied for two cell lines, methanolic extract of C. album (leaves) exhibited maximum antibreast cancer activity having IC(50) (the concentration of an individual compound leading to 50% inhibition) value 27.31 mg/ml against MCF-7 cell line. Significant percent inhibition (94.06%) in the MeOH extract of C. album (leaves) at 48 h of exposure and concentration 100 mg/ml (p < 0.05) against MCF-7 breast cancer cell line, indicates the presence of some structural moiety responsible for this observed antiproliferative effect. In vivo study and structural elucidation of its bioactive principle are in progress. Our findings highlight the potential of this plant for its possible clinical use to counteract malignancy development as antibreast cancer bioagent.
authors.affiliation: Department of Chemistry, Faculty of Science, Dayalbagh Educational Institute, Agra, India.
authors.forename: Menka
authors.initials: M
authors.lastname: Khoobchandani
authors.forename: B K
authors.initials: BK
authors.lastname: Ojeswi
authors.forename: Bhavna
authors.initials: B
authors.lastname: Sharma
authors.forename: Man Mohan
authors.initials: MM
authors.lastname: Srivastava
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use / Q000633:toxicity*; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D027463:Chenopodium album / Q000737:chemistry*; D005260:Female; D006801:Humans; D010936:Plant Extracts / Q000627:therapeutic use / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts