Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs.
The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.
Bonnin
Brigitte
B
Gen-Probe Diaclone SAS, 1 Boulevard A. Fleming, 25020 Besançon, France.
Correll
Anita
A
Swiatek-de Lange
Magdalena
M
Lenter
Martin
M
Schneider
Franz-Joseph
FJ
Wijdenes
John
J
Jonuleit
Helmut
H
Becker
Christian
C
D015704:CD4 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000096:biosynthesis; D057166:High-Throughput Screening Assays; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006801:Humans; D007108:Immune Tolerance; D018189:Immunomagnetic Separation; D039041:Integrin alpha4beta1 / Q000096:biosynthesis*; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D007937:Leukapheresis; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D015704:CD4 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D000949:Histocompatibility Antigens Class II; D039041:Integrin alpha4beta1; D053645:Interleukin-2 Receptor alpha Subunit