title: Antiseptic efficacy and tolerance of tissue-tolerable plasma compared with two wound antiseptics on artificially bacterially contaminated eyes from commercially slaughtered pigs.
abstract: OBJECTIVE
  To compare the tissue tolerance and efficacy of two wound antiseptics with tissue-tolerable plasma (TTP) on enucleated contaminated eyes from slaughtered pigs in order to draw consequences for the use of TTP on wounds.
  
  
  METHODS
  The corneas of extracted eyes were contaminated with Staphylococcus aureus or Pseudomonas aeruginosa. One and 10 min after application of 10% povidone (PVP)-iodine and 0.04% polyhexanide, respectively, the eyes were rinsed with inactivating solution. To test TTP, the plasma pen meandered over the eyes at a speed of 30 mm/s and a distance of 5 mm; the eyes were then rinsed with balanced salt solution. The reduction factor was calculated by the difference between the logarithm of colony-forming units in the rinse before and after antisepsis or TTP application.
  
  
  RESULTS
  The efficacy of TTP (reduction factor 2.4-2.9) was significantly higher (p < 0.001) than that of PVP-iodine and polyhexanide (reduction factor 1.7-2.1).
  
  
  CONCLUSIONS
  TTP is more effective than the tested wound antiseptics. The lack of histological damage to the eyes of slaughtered pigs would seem to make its use as a wound antiseptic a viable alternative. In contrast to antiseptics, it supplies additional energy in the form of heat, electric fields and radicals by TTP.
authors.affiliation: Institute of Hygiene and Environmental Medicine, University of Greifswald, Greifswald, Germany. kramer@uni-greifswald.de
authors.forename: A
authors.initials: A
authors.lastname: Hammann
authors.forename: N-O
authors.initials: NO
authors.lastname: Huebner
authors.forename: C
authors.initials: C
authors.lastname: Bender
authors.forename: A
authors.initials: A
authors.lastname: Ekkernkamp
authors.forename: B
authors.initials: B
authors.lastname: Hartmann
authors.forename: P
authors.initials: P
authors.lastname: Hinz
authors.forename: E
authors.initials: E
authors.lastname: Kindel
authors.forename: I
authors.initials: I
authors.lastname: Koban
authors.forename: S
authors.initials: S
authors.lastname: Koch
authors.forename: T
authors.initials: T
authors.lastname: Kohlmann
authors.forename: J
authors.initials: J
authors.lastname: Lademann
authors.forename: R
authors.initials: R
authors.lastname: Matthes
authors.forename: G
authors.initials: G
authors.lastname: Müller
authors.forename: R
authors.initials: R
authors.lastname: Titze
authors.forename: K-D
authors.initials: KD
authors.lastname: Weltmann
authors.forename: A
authors.initials: A
authors.lastname: Kramer
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology* / Q000633:toxicity; D000985:Antisepsis; D001419:Bacteria / Q000187:drug effects*; D001645:Biguanides / Q000494:pharmacology* / Q000633:toxicity; D015169:Colony Count, Microbial; D003315:Cornea / Q000382:microbiology*; D058626:Plasma Gases / Q000494:pharmacology*; D011206:Povidone-Iodine / Q000494:pharmacology* / Q000633:toxicity; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D014947:Wounds and Injuries / Q000382:microbiology*
chemical_list: D000891:Anti-Infective Agents, Local; D001645:Biguanides; D058626:Plasma Gases; C031233:polihexanide; D011206:Povidone-Iodine