[Karyotype analysis of Gentiana straminea].
OBJECTIVE
Study on chromosome number, karyotype of Gentiana straminea for the first time. Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica. Provide cytological evidence for further studies on genetics and evolution.


METHODS
Soak the root tip in 0.002 mol/L 8-oxychinolin solution for 5.7 h. Decomposed in 2.5% mixed enzyme solution for 1.6 h at 25 degrees C and use Hypotonic treatment for 3 h in refrigerator. At last, make specimen slides by the Air-drying technique. Sections combined with micrograph were used to analyze chromosome.


RESULTS
The karyotypes formula of Gentiana straminea is K(2n) = 26 = 2M + 24 m, the AS. K was 52.68%, which belong to "1A" type.


CONCLUSIONS
Compare karyotypes of Gentiana straminea, Gentiana macrophylla and Gentiana dahurica, the result showed that Gentiana macrophylla lives in highest stage of evolution. Gentiana straminea is intermediate between Gentiana macrophylla and Gentiana dahurica.
Wang
Lan
L
Gansu College of TCM, Lanzhou 730000, China.
Liu
Li-Sha
LS
Wu
Di
D
An
Fang-yu
FY
D032461:Chromosomes, Plant / Q000235:genetics*; D030012:Gentiana / Q000145:classification / Q000235:genetics*; D007621:Karyotyping; D010784:Photomicrography; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D013045:Species Specificity