title: Gaussia luciferase reporter assay for assessment of gene delivery systems in vivo.
abstract: OBJECTIVE
  To develop an alternative method for assessment of gene delivery systems in vivo.
  
  
  METHODS
  Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected.
  
  
  RESULTS
  As little as 10 muL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.
  
  
  CONCLUSIONS
  Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xu
authors.forename: Jiang
authors.initials: J
authors.lastname: Lu
authors.forename: Xiang
authors.initials: X
authors.lastname: Lü
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Mu
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: De-pei
authors.initials: DP
authors.lastname: Liu
authors.forename: Chih-chuan
authors.initials: CC
authors.lastname: Liang
mesh_terms: D000818:Animals; D028023:Arecaceae / Q000201:enzymology*; D002460:Cell Line; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics*; D051379:Mice
chemical_list: D008156:Luciferases