A developed DNA extraction method for different soil samples.
Four DNA extraction methods namely SDS-hyperhaline method (I), modified SDS-hyperhaline method (II), indirect method (III), alkaline lysis method (IV) were evaluated by comparing DNA yield, spectrophotometric quality, genomic integrity and PCR suitability in this paper. The results showed that high DNA yields were obtained by method I, II and IV. However, higher quality of DNA was gained by method III and IV. Based on the results of the Pulsed-Field Gel Electrophoresis (PFGE), the completeness of DNA extracted by method IV was the best. About 6.0 microg DNA can be recovered from 1.0 g soil by method IV which involved to lysis cell by SDS and to precipitate impurities by adding potassium acetate and magnesium chloride Therefore, it is confirmed that method IV is a novel, reliable and versatile method for large-scale DNA extraction involving less purification steps for various soil samples.
Hu
Yingchang
Y
Plant Protection College, Shenyang Agriculture University, Shenyang, China.
Liu
Zhiheng
Z
Yan
Jianfang
J
Qi
Xiaohui
X
Li
Jing
J
Zhong
Shiqi
S
Yu
Jicheng
J
Liu
Qiu
Q
D004269:DNA, Bacterial / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012987:Soil