title: Identification of inflammation-related proteins in a murine colitis model by 2D fluorescence difference gel electrophoresis and mass spectrometry.
abstract: OBJECTIVE
  The aim of this study was to identify new intestinal proteins potentially associated with acute inflammation using proteomic profiling of an in vivo mice model of ulcerative colitis.
  
  
  METHODS
  2D fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight spectrometer (MALDI-TOF) peptide mass fingerprinting were used to determine differentially expressed proteins between normal and inflamed intestinal mucosa. Acute colitis was induced by 8.0% dextran sodium sulfate (DSS) given p.o. for 7 days.
  
  
  RESULTS
  Among a total of seven protein spots showing differential expression, we identified five different proteins, of which two were upregulated and three downregulated in colitis in comparison to normal mucosa, using the MASCOT search engine. 3-Hydroxy-3-methylglutaryl-coenzyme A synthase 2 and serpin b1a were upregulated proteins, and protein disulfide-isomerase A3, peroxiredoxin-6 and vimentin were identified as downregulated proteins.
  
  
  CONCLUSIONS
  These identified proteins may be responsible for the development of the intestinal inflammation. 2D-DIGE and MALDI-TOF mass spectrometry are useful in the search for the differentially expressed proteins.
authors.affiliation: Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto, Japan. ynaito@koto.kpu-m.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Naito
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Takagi
authors.forename: Hitomi
authors.initials: H
authors.lastname: Okada
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Omatsu
authors.forename: Katsura
authors.initials: K
authors.lastname: Mizushima
authors.forename: Osamu
authors.initials: O
authors.lastname: Handa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kokura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Hideshi
authors.initials: H
authors.lastname: Fujiwake
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005453:Fluorescence; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010449:Peptide Mapping; D054465:Peroxiredoxin VI / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015843:Serpins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014746:Vimentin / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D011506:Proteins; C522950:Serpinb1a protein, mouse; D015843:Serpins; D014746:Vimentin; D016264:Dextran Sulfate; D054465:Peroxiredoxin VI; C515983:Prdx6 protein, mouse; D006904:Hydroxymethylglutaryl-CoA Synthase; C513195:Pdia3 protein, mouse; D019704:Protein Disulfide-Isomerases