Triterpene esters from Uncaria rhynchophylla drive potent IL-12-dependent Th1 polarization.
Dendritic cells (DC) are key antigen-presenting cells that link innate and adaptive immunity and ultimately activate antigen-specific T cells. In the current study, we demonstrated that two triterpene esters, uncarinic acid C (1) and uncarinic acid D (2), which are isolated from the hooks of Uncaria rhynchophylla, activate phenotypic and cytokine production alterations in DC. We also show that 1 and 2 modulate human DC function in a fashion that favors Th1 cell polarization. The effect of 1 (E configuration at the 2' position) was approximately 20 times more potent than that of 2 (Z configuration at 2'). These results indicated that the configuration of the 2' double bond greatly effects activity. Thus, 1 and 2 may prove useful as DC-based vaccines for cancer immunotherapy.
Umeyama
Akemi
A
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan. umeyama@ph.bunri-u.ac.jp
Yahisa
Yoshinori
Y
Okada
Minori
M
Okayama
Eriko
E
Uda
Ayaka
A
Shoji
Noboru
N
Lee
Je-Jung
JJ
Takei
Masao
M
Hashimoto
Toshihiro
T
D016764:Cell Polarity / Q000187:drug effects* / Q000502:physiology; D004952:Esters; D018922:HL-60 Cells; D006801:Humans; D018664:Interleukin-12 / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018514:Plant Structures; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D032070:Uncaria
D004952:Esters; D010936:Plant Extracts; D014315:Triterpenes; C412669:uncarinic acid-C; C412670:uncarinic acid-D; D018664:Interleukin-12