Clostridium difficile toxinotyping.
Clostridium difficile shows considerable variability in the PaLoc region encoding two main virulence factors, toxins TcdA and TcdB. Strains with changes in PaLoc are defined as variant toxinotypes and currently 27 such groups are recognized (I to XXVII). Toxinotype 0 includes strains with PaLoc identical to the reference laboratory strain VPI 10463. Toxinotyping is a RFLP-PCR-based method using a combination of restriction patterns of part of tcdB and tcdA genes for determination of toxinotype. Variations in PaLoc can affect the toxin production or could result in production of toxins with altered properties.
Rupnik
Maja
M
Institute of Public Health Maribor, Centre for Microbiology, Maribor, Slovenia.
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000145:classification / Q000235:genetics; D015373:Bacterial Typing Techniques; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile