title: Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei.
abstract: The biological membranes of Trypanosoma brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of approximately 500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.
authors.affiliation: Centre for Biomolecular Sciences, The North Haugh, The University, St. Andrews, KY16 9ST, Scotland, U.K.
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Richmond
authors.forename: Federica
authors.initials: F
authors.lastname: Gibellini
authors.forename: Simon A
authors.initials: SA
authors.lastname: Young
authors.forename: Louise
authors.initials: L
authors.lastname: Major
authors.forename: Helen
authors.initials: H
authors.lastname: Denton
authors.forename: Alison
authors.initials: A
authors.lastname: Lilley
authors.forename: Terry K
authors.initials: TK
authors.lastname: Smith
mesh_terms: D006801:Humans; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D013058:Mass Spectrometry; D010712:Phosphatidic Acids / Q000032:analysis / Q000737:chemistry; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000032:analysis / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000032:analysis / Q000737:chemistry; D010716:Phosphatidylinositols / Q000032:analysis / Q000737:chemistry; D010718:Phosphatidylserines / Q000032:analysis / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D014352:Trypanosomiasis / Q000469:parasitology*
chemical_list: D008055:Lipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; C483858:phosphatidylethanolamine