CGP stain: An inexpensive, odorless, rapid, sensitive, and in principle in vitro methylation-free Coomassie Brilliant Blue stain.
Coomassie Brilliant Blue (CBB) protein stains are inexpensive but detect proteins at only at microgram levels. Because of acetic acid and methanol, they cause skin irritation and reduce work motivation by malodor. Recent mass spectrometric (MS) analyses demonstrated that nanogram-sensitive colloidal CBB staining resulted in in vitro methylations of proteins. We propose a rapid, inexpensive, sensitive, odorless, less harsh, and in vitro methylation-free CBB stain. CGP uses three components: citric acid, CBB G-250, and polyvinylpyrrolidone. CGP detects proteins at 12ng within 45min, and because it is nonalcohol, in principle in vitro methylation would be eliminated. Indeed, MS analysis of CGP-stained bands confirmed a lack of methylation.
Yasumitsu
Hidetaro
H
Department of Environmental Biosciences, Yokohama City University, Kanagawa, Japan. hyasumit@yokohama-cu.ac.jp
Ozeki
Yasuhiro
Y
Kawsar
Sarkar M A
SM
Toda
Tosifusa
T
Kanaly
Robert
R
D000818:Animals; D002417:Cattle; D004396:Coloring Agents / Q000191:economics* / Q000378:metabolism / Q000633:toxicity; D009812:Odorants; D011506:Proteins / Q000378:metabolism; D012394:Rosaniline Dyes / Q000191:economics* / Q000378:metabolism / Q000633:toxicity; D013194:Staining and Labeling / Q000191:economics* / Q000379:methods*; D013997:Time Factors
D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue