In vitro and in vivo studies of FePt nanoparticles for dual modal CT/MRI molecular imaging.
The water-solvable FePt nanoparticles of 3, 6, and 12 nm in diameter (3 nm-, 6 nm-, and 12 nm-FePt) were synthesized and applied as a dual modality contrast agent for CT/MRI molecular imaging. These nanoparticles present excellent biocompatibility and hemocompatibility in all test concentrations for the imaging contrast. The biodistribution analysis revealed the highest serum concentration and circulation half-life for 12 nm-FePt, followed by 6 nm-FePt then 3 nm-FePt. Thus, the 3 nm-FePt showed higher brain concentrations. Anti-Her2 antibody conjugated FePt nanoparticles demonstrated molecular expression dependent CT/MRI dual imaging contrast effect in MBT2 cell line and its Her2/neu gene knock out counterpart. Selective contrast enhancement of Her2/neu overexpression cancer lesions in both CT and MRI was found in tumor bearing animal after tail vein injection of the nanoparticles. The 12 nm-FePt outperformed 3 nm-FePt in both imaging modalities. These results indicate the potential of FePt nanoparticles to serve as novel multimodal molecular imaging contrast agents in clinical settings.
Chou
Shang-Wei
SW
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Shau
Yu-Hong
YH
Wu
Ping-Ching
PC
Yang
Yu-Sang
YS
Shieh
Dar-Bin
DB
Chen
Chia-Chun
CC
D000818:Animals; D045744:Cell Line, Tumor; D055786:Gene Knockout Techniques; D018734:Genes, erbB-2; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D008297:Male; D053768:Metal Nanoparticles; D051379:Mice; D008809:Mice, Inbred C3H; D046529:Microscopy, Electron, Transmission; D010984:Platinum / Q000737:chemistry*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed; D014961:X-Ray Diffraction
D010984:Platinum; D007501:Iron