Caldilinea tarbellica sp. nov., a filamentous, thermophilic, anaerobic bacterium isolated from a deep hot aquifer in the Aquitaine Basin.
An anaerobic, thermophilic, filamentous (0.45 × >100 µm) bacterium, designated D1-25-10-4(T), was isolated from a deep hot aquifer in France. Cells were non-motile and Gram-negative. Growth was observed at 43-65 °C (optimum 55 °C), at pH 6.8-7.8 (optimum pH 7.0) and with 0-5 g NaCl l(-1) (optimum 0 g NaCl l(-1)). Strain D1-25-10-4(T) was a chemo-organotroph and fermented ribose, maltose, glucose, galactose, arabinose, fructose, mannose, sucrose, raffinose, xylose, glycerol, fumarate, peptone, starch and xylan. Yeast extract was required for growth. Sulfate, thiosulfate, sulfite, elemental sulfur, nitrate, nitrite and fumarate were not used as terminal electron acceptors. The G+C content of the DNA was 61.9 mol%. The major cellular fatty acids of strain D1-25-10-4(T) were C(17 : 0), C(18 : 0,) C(16 : 0) and iso-C(17 : 0). The closest phylogenetic relative of strain D1-25-10-4(T) was Caldilinea aerophila STL-6-O1(T) (97.9 % 16S rRNA gene sequence similarity). DNA-DNA relatedness between strain D1-25-10-4(T) and Caldilinea aerophila DSM 14535(T) was 8.7 ± 1 %. On the basis of phylogenetic, genotypic and phenotypic characteristics, strain D1-25-10-4(T) represents a novel species within the genus Caldilinea, class Caldilineae, phylum Chloroflexi, for which the name Caldilinea tarbellica sp. nov. is proposed. The type strain is D1-25-10-4(T) ( = DSM 22659(T)  = JCM 16120(T)).
Grégoire
Patrick
P
Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.|Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
Bohli
Malek
M
Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
Cayol
Jean-Luc
JL
Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
Joseph
Manon
M
Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
Guasco
Sophie
S
LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
Dubourg
Karine
K
Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.
Cambar
Jean
J
Institut du Thermalisme, Université Victor Segalen Bordeaux 2, 8 rue Sainte Ursule, 40100 Dax, France.
Michotey
Valérie
V
LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
Bonin
Patricia
P
LMGEM, UMR 6117, Centre d'Océanologie de Marseille, Université Aix Marseille 2 Campus de Luminy - Case 901, 163 Avenue de Luminy, 13288 Marseille, France.
Fardeau
Marie-Laure
ML
Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
Ollivier
Bernard
B
Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds UMR 180, IRD, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288 Marseille, France.
D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005602:France; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D014871:Water Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D012965:Sodium Chloride