Characterization of bacterial lipid profiles by using rapid sample preparation and fast comprehensive two-dimensional gas chromatography in combination with mass spectrometry.
The bacteria fatty acid profile has been extensively studied for taxonomic classification purposes, since bacteria, in general, contain particular and rare fatty acids, compared with animal and plant tissues. As for any real-world sample type, the development of rapid and reliable methods for (i) sample identification (in this case, bacterium type), and (ii) constituent identification (in this instance, the fatty acid profile) is desirable. In this research, a half-an-hour procedure, to analyze bacteria, was developed: a 2-min one-step sample preparation step was followed by a relatively fast comprehensive 2D GC-MS separation (25 min). Furthermore, dedicated MS libraries were constructed for the identification of bacteria and fatty acids. Finally, data processing, only qualitative at this stage, was carried out with the support of a novel comprehensive 2D GC software.
Purcaro
Giorgia
G
Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy.
Tranchida
Peter Quinto
PQ
Dugo
Paola
P
La Camera
Erminia
E
Bisignano
Giuseppe
G
Conte
Lanfranco
L
Mondello
Luigi
L
D001412:Bacillus subtilis / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000737:chemistry*; D012984:Software; D013211:Staphylococcus aureus / Q000737:chemistry*; D013997:Time Factors
D005227:Fatty Acids