title: [Antidysmenorrheic effects of Radix angelica and Rhizoma Chuanxiong with different proportions and preparation methods on dysmenorrhea model mice].
abstract: OBJECTIVE
  To study the effect of Radix Angelica and Rhizoma Chuanxiong with different proportions (1 : 0, 2 : 1, 1.5 : 1, 1 : 1, 1 : 1.5, 1 : 2, 0 : 1), different extraction in order (by water, by ethanol, firstly by alcohol then by water) on dysmenorrhea model mice in order to observe their compatibility rules and material foundation.
  
  
  METHODS
  The mice model of primary dysmenorrhea was used to study the effect of Radix Angelica and Rhizoma Chuanxiong with different proportions and preparation methods on writhing response and the levels of nitric oxide and calcium ion in mice uterine tissue.
  
  
  RESULTS
  Danggui-Chuanxiong (1. 5 : 1) and solution extracted firstly by alcohol then by water showed the strongest effect on primary dysmenorrhea by reducing the writhing times, increasing nitric oxide (NO) concentration and reducing calcium ion (Ca2+) concentration in uterine tissue.
  
  
  CONCLUSIONS
  Radix Angelica and Rhizoma Chuanxiong with different proportions and preparation methods showed different antidysmenorrheic trend on dysmenorrhea model mice, which was related with the containing constituents.
authors.affiliation: Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
authors.forename: Weixia
authors.initials: W
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D001522:Behavior, Animal / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002626:Chemistry, Pharmaceutical; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D004412:Dysmenorrhea / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005260:Female; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; C534193:chuanxiong rhizome preparation; D009569:Nitric Oxide; D002118:Calcium