Comparison of the cardioprotective effect of deoxygenated hemoglobin-based oxygen carrier (HBOC) pretreatment and ischemia preconditioning.
This study was designed to compare the influence of deoxygenated hemoglobin-based oxygen carrier (HBOC) pretreatment and ischemia preconditioning on cardiac ischemia/reperfusion (I/R) injury. Langendorff-perfused rat hearts were pretreated with 0.1 gHb/dL deoxygenated HBOC or ischemia, then subjected to 50-min warm ischemia and 2-hr reperfusion. The results indicated that deoxygenated HBOC pretreatment and ischemia preconditioning both equally improved the recovery of cardiac function, and reduced the cardiac enzyme release and myocardial histopathological changes as compared to the control group. Therefore our study demonstrated that deoxygenated HBOC pretreatment and ischemia precondition provided equivalent protection to the isolated heart against I/R injury.
You
Zhen
Z
Department of Hepato-Bilio-Pancreatology, West China Hospital, Sichuan University, Chengdu, China.
Li
Tao
T
Yang
Chengmin
C
Wu
Hong
H
Zeng
Yong
Y
D000818:Animals; D002316:Cardiotonic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control; D013696:Temperature; D016277:Ventricular Function, Left / Q000187:drug effects
D002316:Cardiotonic Agents; D006454:Hemoglobins; C100003:deoxyhemoglobin; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form; D010100:Oxygen