title: Analysis of methylation patterns and expression profiles of p14ARF gene in patients with oral squamous cell carcinoma.
abstract: OBJECTIVE
  To analyze the promoter methylation profile and mRNA expression of the p14ARF gene in oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Promoter methylation of the p14ARF gene was investigated by methylation-specific polymerase chain reaction in paraffin-embedded tissues from 76 patients with OSCC and 57 oral tissues used as healthy controls. Expression of p14ARF mRNA was also determined using real-time quantitative reverse-transcription PCR. The methylation status and mRNA level profile of the gene and their relationship with clinical data were analyzed.
  
  
  RESULTS
  Methylation of the p14ARF gene in OSCC was significantly increased compared to normal control tissues (chi(2) = 16.73, p < 0.0001). The relative expression of p14ARF mRNA in OSCC was not significantly different from that in healthy control samples.
  
  
  CONCLUSIONS
  Promoter methylation of p14ARF may be an important mechanism in OSCC, and its determination may be considered an important tool in the early diagnosis and treatment of OSCC.
authors.affiliation: Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
authors.forename: Dor Mohammad
authors.initials: DM
authors.lastname: Kordi-Tamandani
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Moazeni-Roodi
authors.forename: Mohammad Ayoub
authors.initials: MA
authors.lastname: Rigi Ladez
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Elnaz
authors.initials: E
authors.lastname: Birjandian
authors.forename: Adam
authors.initials: A
authors.lastname: Torkamanzehi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D019175:DNA Methylation; D055088:Early Detection of Cancer; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics* / Q000378:metabolism
chemical_list: D025803:Tumor Suppressor Protein p14ARF