High throughput preparation and characterisation of amphiphilic nanostructured nanoparticulate drug delivery vehicles.
The preparation, characterisation and assessment of drug delivery vehicles is typically a slow and complex process. Here we present a nanostructured nanoparticle system that can be prepared and characterised in a high-throughput fashion. In particular we use phytantriol and Myverol to prepare inverse bicontinuous cubic and inverse hexagonal liquid crystalline nanoparticles loaded with 10 commonly used therapeutic agents at increasing concentration. The dispersions are prepared using automated apparatus to create different concentrations and phases using novel protocols. We are able to characterise each stabilised nanoparticle dispersion using a range of methodologies including small angle X-ray scattering, particle sizing and drug partitioning. With this information we are able to assess which drug delivery vehicle is preferred for each drug and at which concentration the drug should be loaded to ensure maximum payload and to retain particle integrity.
Mulet
Xavier
X
CSIRO Molecular and Health Technologies, Bag 10, Clayton South MDC, VIC 3169, Australia.
Kennedy
Danielle F
DF
Conn
Charlotte E
CE
Hawley
Adrian
A
Drummond
Calum J
CJ
D057205:Automation, Laboratory; D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004355:Drug Stability; D005233:Fatty Alcohols / Q000737:chemistry*; D005989:Glycerides / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D050866:Liquid Crystals / Q000737:chemistry*; D053758:Nanoparticles; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000737:chemistry; D053838:Scattering, Small Angle; D012995:Solubility; D013053:Spectrophotometry; D013678:Technology, Pharmaceutical / Q000379:methods*; D014961:X-Ray Diffraction
D004337:Drug Carriers; D005233:Fatty Alcohols; D005989:Glycerides; D004364:Pharmaceutical Preparations; C508873:3,7,11,15-tetramethyl-1,2,3-hexadecanetriol; C005953:monoolein