Influence of solute charge and hydrophobicity on partitioning and diffusion in a genetically engineered silk-elastin-like protein polymer hydrogel.
The influence of solute hydrophobicity and charge on partitioning and diffusion in physically crosslinked networks of a genetically engineered SELP polymer was investigated. A series of fluorescent dyes were used to assess the impact of solute charge and hydrophobicity on release behavior. The mechanism of solute release from the SELP hydrogel appeared to vary as a function of dye hydrophobicity. The extent of FITC attachment to amine-terminated G4 dendrimers influenced SELP hydrogel partitioning more than dendrimer diffusion properties. Results suggest the possibility of controlling solute release from SELP hydrogels by modifying the hydrophobicity and surface charge of drugs and drug/polymer conjugates as well as the possibility of "designing-in" solute-specific interactions.
Dinerman
Adam A
AA
Department of Pharmaceutical Sciences, University of Maryland, 20  N. Pine Street, Baltimore, MD, USA.
Cappello
Joseph
J
El-Sayed
Mohamed
M
Hoag
Stephen W
SW
Ghandehari
Hamidreza
H
D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry; D004058:Diffusion; D004549:Elastin / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D005818:Genetic Engineering / Q000379:methods*; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D047011:Silk / Q000737:chemistry / Q000235:genetics; D012996:Solutions / Q000737:chemistry
D001672:Biocompatible Materials; D050091:Dendrimers; D005456:Fluorescent Dyes; D020100:Hydrogels; C104700:PAMAM Starburst; D011108:Polymers; D047011:Silk; D012996:Solutions; D004549:Elastin