Interdomain conjugal transfer of DNA from bacteria to archaea.
Escherichia coli transforms the methanogenic archaeon Methanococcus maripaludis at frequencies ranging from 0.2 x 10(-6) to 2 x 10(-6) per recipient cell. Transformation requires cell-to-cell contact, oriT, and tra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.
Dodsworth
Jeremy A
JA
School of Life Sciences, University of Nevada-Las Vegas, Las Vegas, Nevada 89154, USA.
Li
Lei
L
Wei
Shiping
S
Hedlund
Brian P
BP
Leigh
John A
JA
de Figueiredo
Paul
P
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D005822:Genetic Vectors; D017017:Methanococcus / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial