title: Gap junction-mediated spontaneous Ca(2+) waves in differentiated cholinergic SN56 cells.
abstract: Neuronal gap junctions are receiving increasing attention as a physiological means of intercellular communication, yet our understanding of them is poorly developed when compared to synaptic communication. Using microfluorimetry, we demonstrate that differentiation of SN56 cells (hybridoma cells derived from murine septal neurones) leads to the spontaneous generation of Ca(2+) waves. These waves were unaffected by tetrodotoxin (1microM), but blocked by removal of extracellular Ca(2+), or addition of non-specific Ca(2+) channel inhibitors (Cd(2+) (0.1mM) or Ni(2+) (1mM)). Combined application of antagonists of NMDA receptors (AP5; 100microM), AMPA/kainate receptors (NBQX; 20microM), nicotinic AChR receptors (hexamethonium; 100microM) or inotropic purinoceptors (brilliant blue; 100nM) was also without effect. However, Ca(2+) waves were fully prevented by carbenoxolone (200microM), halothane (3mM) or niflumic acid (100microM), three structurally diverse inhibitors of gap junctions, and mRNA for connexin 36 was detected by PCR. Whole-cell patch-clamp recordings revealed spontaneous inward currents in voltage-clamped cells which we inhibited by Cd(2+), Ni(2+) or niflumic acid. Our data suggest that differentiated SN56 cells generated spontaneous Ca(2+) waves which are propagated by intercellular gap junctions. We propose that this system can be exploited conveniently for the development of neuronal gap junction modulators.
authors.affiliation: Faculty of Medicine and Health, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Nishani T
authors.initials: NT
authors.lastname: Hettiarachchi
authors.forename: Mark L
authors.initials: ML
authors.lastname: Dallas
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Pearson
authors.forename: Gareth
authors.initials: G
authors.lastname: Bruce
authors.forename: Susan
authors.initials: S
authors.lastname: Deuchars
authors.forename: John P
authors.initials: JP
authors.lastname: Boyle
authors.forename: Chris
authors.initials: C
authors.lastname: Peers
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002460:Cell Line; D017629:Gap Junctions / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism
chemical_list: D011978:Receptors, Nicotinic; D000109:Acetylcholine; D002118:Calcium