title: Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites.
abstract: In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.
authors.affiliation: Department of Molecular Biology, Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden. hyun-sook.park@molbiol.umu.se
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Park
authors.forename: Yngve
authors.initials: Y
authors.lastname: Ostberg
authors.forename: Jörgen
authors.initials: J
authors.lastname: Johansson
authors.forename: E Gerhart H
authors.initials: EG
authors.lastname: Wagner
authors.forename: Bernt Eric
authors.initials: BE
authors.lastname: Uhlin
mesh_terms: D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D029968:Escherichia coli Proteins; C529458:FimG protein, E coli; D036781:Fimbriae Proteins