Dengue-2-virus-interacting polypeptides involved in mosquito cell infection.
For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus-host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.
Paingankar
Mandar S
MS
Molecular Biology Research Laboratory, Department of Zoology, Centre for Advance Studies, University of Pune, Pune, India.
Gokhale
Mangesh D
MD
Deobagkar
Dileep N
DN
D000330:Aedes / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D000818:Animals; D002460:Cell Line; D003715:Dengue; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007303:Insect Vectors / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
D019476:Insect Proteins; D010455:Peptides