title: Adipose tissue-derived stem cells for cell therapy of airway allergic diseases in mouse.
abstract: The purpose of this study was to compare murine mesenchymal stem cells (MSCs) isolated from bone marrow (BM) and adipose tissue (AT) for the selection of suitable MSCs in cell therapy of an airway allergic animal model. We compared MSCs of BALB/c mice derived from BM and AT with respect to proliferation potential, immunophenotype, and multilineage differentiation capacity. In proliferation potential, MSCs from AT (ASCs) showed higher fibroblastoid colony-forming units frequencies and colony-forming efficiency than MSCs from BM (BMSCs). The flow cytometry analysis showed that both ASCs and BMSCs expressed MSCs-related antigens (CD90 and CD105), whereas they did not express hematopoiesis-related antigens (CD45 and CD11b). There was no significant difference in adipogenic, osteogenic, and chondrogenic differentiation between the murine ASCs and BMSCs. In conclusion, the present study has shown that ASCs had higher CFU-F frequencies and colony-forming efficiency than BMSCs. ASCs and BMSCs presented a similar surface immunophenotype and multilineage differentiation capacity. Therefore, ASCs in BALB/c mice might be a more useful material for cell therapy of the airway allergic experiment due to the abundance, relatively easy harvesting and high proliferation potential.
authors.affiliation: Department of Otorhinolaryngology and Medical Research Institute, Pusan National University School of Medicine, Busan, South Korea.
authors.forename: Hyeong-Jun
authors.initials: HJ
authors.lastname: Jang
authors.forename: Kyu-Sup
authors.initials: KS
authors.lastname: Cho
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Hwan-Jung
authors.initials: HJ
authors.lastname: Roh
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D048430:Cell Shape; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D003114:Colony-Forming Units Assay; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010011:Osteocytes / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D012130:Respiratory Hypersensitivity / Q000628:therapy*
chemical_list: D015703:Antigens, CD; D024043:Collagen Type II; D000469:Alkaline Phosphatase; D002118:Calcium