Dual effects of alpha-synuclein on neurotoxicity induced by low dosage of rotenone are dependent on exposure time in dopaminergic neuroblastoma cells.
This study was designed to investigate the effects of alpha-synuclein on toxicity induced by long-term exposure to relatively low concentrations of rotenone. Compared with the control groups, the inhibition of cell viability which overexpressed alpha-synuclein (SH-SY5Y-Syn) improved after 1 and 2 weeks of rotenone treatment. The complex I activity was greater and the mitochondrial membrane swelling intensity was reduced after 1 and 2 weeks of treatment, which indicated that alpha-synuclein, at least in part, resists the rotenone-induced oxidative stress. The results indicate that alpha-synuclein has a dual effect on toxicity of rotenone according to exposure time in human SH-SY5Y cells.
Lu
Lingling
L
Beijing Institute for Neuroscience, Capital Medical University, The Beijing Center of Neural Regeneration and Repairing, Key Laboratory for Neurodegenerative Disease of the Ministry of Education, Beijing, 100069, China.
Gu
Li
L
Liang
Yuan
Y
Sun
Xiaohong
X
Duan
Chunli
C
Yang
Hui
H
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008933:Mitochondrial Swelling / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D012402:Rotenone / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D013997:Time Factors; D014162:Transfection; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
D007306:Insecticides; D051844:alpha-Synuclein; D012402:Rotenone; D010100:Oxygen; D004298:Dopamine