title: The Escherichia coli glycophage display system.
abstract: We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylation.
authors.affiliation: Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.
authors.forename: Clemens
authors.initials: C
authors.lastname: Dürr
authors.forename: Harald
authors.initials: H
authors.lastname: Nothaft
authors.forename: Christian
authors.initials: C
authors.lastname: Lizak
authors.forename: Rudi
authors.initials: R
authors.lastname: Glockshuber
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
mesh_terms: D003090:Coliphages / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000821:virology; D006031:Glycosylation