[Isolation and identification of antineoplastic cyclic peptide].
OBJECTIVE
To isolate and identify the antineoplastic constituents of the marine algae Galaxaura filamentosa.


METHODS
The constituents were isolated and purified by low-pressure silica gel column chromatography, thin layer chromatography and recrystallization, and their structures were elucidated by spectroscopic analysis.


RESULTS
Isolated a new antineoplastic cyclic peptide, cyclo-N-methyl-Leucine-Leucine-N-methyl-Leucine-Leucine-Leucine.


CONCLUSIONS
This new compound was remarkably active against the human renal cell carcinoma GRC-1 and human hepatocellular carcinoma HepG2 cell lines with corresponding IC50 values of 4.26 microg/mL and 4.63 microg/mL.
Liang
Qiu
Q
Department of Chemistry, Jian University, Guangzhou 510632, China.
Liao
Xiao-jian
XJ
Xu
Shi-hai
SH
Xu
Wen-jie
WJ
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D000461:Rhodophyta / Q000737:chemistry*
D000970:Antineoplastic Agents; D010456:Peptides, Cyclic