[Effect of formula for reinforcing kidney and activating blood on follicular development by rectal administration].
OBJECTIVE
To study the effect of the Zichong Granules (ZG) on follicular development by rectal administration.


METHODS
Underage rats and ovariectomized mice were administered with ZG by clyster perfusion. Diethylstilbestrol and Nvjindan (NJD) were taken as control. Ovarian follicle, corpus luteum of rats were counted. Uterus index and histomorphology of uterus of rats and mice were observed. In addition, E2 in blood of mice were determined by enzyme immunoassay (EIA).


RESULTS
The effects of ZG, Diethylstilbestrol and NJD were similar. They all increased ovarian preantral follicles and total follicles of the rats (P < 0.01 or P < 0.05), uterus index and the thickness of endometrium of the rats (P < 0.05), and increased uterus index (P < 0.01),the thickness of endometrium (P < 0.05) and E2 level in blood (P < 0.05) of the mice.


CONCLUSIONS
ZG administered by clyster perfusion can improve follicular development of underage rats, increase E2 level of ovariectomized mice, and present the estrogen-like effect. The effect of ZG for improving follicular development may be related to improving the level of E2. Rectal administration may be one of the effective ways to stimulate the follicular development.
Duan
Heng
H
College of Traditional Chinese Medicine, Chongqing University of Medical Science, Chongqing 400016, China.
Lu
Hua
H
D000285:Administration, Rectal; D000818:Animals; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D004717:Endometrium / Q000187:drug effects; D004958:Estradiol / Q000097:blood*; D005260:Female; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014599:Uterus / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D004958:Estradiol; D004054:Diethylstilbestrol