Polymorphisms of human nonmetastatic clone 23 type 1 gene and neoplastic lesions of uterine cervix.
OBJECTIVE
Single-nucleotide polymorphisms (SNP) in promoter of human nonmetastatic clone 23 type 1 (nm23-H1) may affect their binding with transcription factors and affect promoter activity as well as gene transcription. Therefore, we investigated the impact of the nm23-H1 gene polymorphisms on the neoplastic lesions of uterine cervix in mid-Taiwan women (women who live in the central area of Taiwan). We expected that women with different genotypes in nm23-H1 polymorphisms, such as rs34214448, rs16949649, or rs2302254, may have different incidences of cervical neoplasia.


METHODS
In total, 366 blood samples were collected from 244 healthy women and 122 patients with cervical neoplasia to analyze 3 nm23-H1 gene single-nucleotide polymorphisms (rs34214448, rs16949649, and rs2302254).


RESULTS
The heterozygous genotypes, TG in rs34214448 or TC in rs16949649, were differentially distributed between patients with cervical neoplasia and normal women (Hommel adjusted P = .0440 and .0435, respectively) as compared to their homozygotes. Moreover, compared to those with wild-type homozygotes and heterozygotes, women with variant homozygotes TT in rs34214448 or CC in rs16949649 exert different distributions between patients with cervical neoplasia and normal women (P = .058 and .058). Interestingly, we found the genotype distribution of rs34214448 has significant association with that of rs16949649 with high consistency.


CONCLUSIONS
Mid-Taiwan women with the polymorphic heterozygotes TG in rs34214448 or TC in rs16949649 of human nonmetastatic clone 23 type 1 promoter have the tendency to develop cervical neoplasia while compared to their homozygous counterparts. However, women with variant homozygotes TT in rs34214448 or CC in rs16949649 exhibit less tendency as compared to those with wild-type homozygotes and heterozygotes.
Feng
Chi-Yen
CY
Department of Surgery, Da Chien General Hospital, Miao-Li County, Taiwan.
Wang
Po-Hui
PH
Tsai
Hsiu-Ting
HT
Tee
Yi-Torng
YT
Ko
Jiunn-Liang
JL
Chen
Shiuan-Chih
SC
Lin
Ching-Yi
CY
Han
Chih-Ping
CP
Yang
Jia-Sin
JS
Liu
Yu-Fan
YF
Lin
Long-Yau
LY
Yang
Shun-Fa
SF
D016009:Chi-Square Distribution; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013624:Taiwan; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human