Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025).
The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8h post inoculation (p.i.) and continued through 38hp.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase.
Nalcacioglu
Remziye
R
Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, Wageningen, The Netherlands.
Dizman
Yesim Akturk
YA
Vlak
Just M
JM
Demirbag
Zihni
Z
van Oers
Monique M
MM
D000595:Amino Acid Sequence; D000818:Animals; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics* / Q000378:metabolism; D017996:Entomopoxvirinae / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D014764:Viral Proteins; D004255:Deoxyribodipyrimidine Photo-Lyase