title: Amebic monocyte locomotion inhibitory factor peptide ameliorates inflammation in CIA mouse model by downregulation of cell adhesion, inflammation/chemotaxis, and matrix metalloproteinases genes.
abstract: OBJECTIVE
  Monocyte locomotion inhibitory factor (MLIF), an amebic peptide with antiinflammatory properties, was evaluated in collagen-induced arthritis (CIA) to test its effects on the onset and acute inflammatory response of arthritis.
  
  
  METHODS
  DBA1/J mice at 8-10 weeks of age were divided into four groups (eight mice per group).
  
  
  METHODS
  The adjuvant group received Freund adjuvant, the CIA group was immunized with collagen II, the MLIF/CIA group received collagen II and MLIF, and the MLIF group received MLIF and Freund adjuvant.
  
  
  METHODS
  All groups were evaluated clinically. Seven weeks after the collagen injection, at the peak of the clinical arthritis score, limb specimens were collected and histological studies and gene expression analysis using microarrays were performed.
  
  
  RESULTS
  MLIF administered weekly as a preventive scheme delayed and reduced the severity of acute arthritis. MLIF induced gene changes in functional categories including adhesion molecules, matrix metalloproteinases, and inflammatory cytokines.
  
  
  CONCLUSIONS
  MLIF could be an interesting new molecule to investigate in the field of rheumatoid arthritis pathogenesis research for its potential to prevent inflammation.
authors.affiliation: Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Susana
authors.initials: S
authors.lastname: Godina-Gonzalez
authors.forename: Janette
authors.initials: J
authors.lastname: Furuzawa-Carballeda
authors.forename: Dolores
authors.initials: D
authors.lastname: Utrera-Barillas
authors.forename: Jorge
authors.initials: J
authors.lastname: Alcocer-Varela
authors.forename: Luis M
authors.initials: LM
authors.lastname: Teran
authors.forename: Monica
authors.initials: M
authors.lastname: Vazquez-del Mercado
authors.forename: Yelda A
authors.initials: YA
authors.lastname: Leal
authors.forename: Isabel
authors.initials: I
authors.lastname: Alvarado-Cabrero
authors.forename: Juan R
authors.initials: JR
authors.lastname: Velazquez
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects*; D002633:Chemotaxis / Q000187:drug effects*; D004195:Disease Models, Animal; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics*; D051379:Mice; D008811:Mice, Inbred DBA; D046228:Microarray Analysis; D009842:Oligopeptides / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D009842:Oligopeptides; C430647:monocyte locomotion inhibitory factor; D020782:Matrix Metalloproteinases