title: Differential gene expression from midguts of refractory and susceptible lines of the mosquito, Aedes aegypti, infected with Dengue-2 virus.
abstract: Suppressive subtractive hybridization was used to evaluate the differential expression of midgut genes of feral populations of Aedes aegypti (Diptera: Culicidae) from Colombia that are naturally refractory or susceptible to Dengue-2 virus infection. A total of 165 differentially expressed sequence tags (ESTs) were identified in the subtracted libraries. The analysis showed a higher number of differentially expressed genes in the susceptible Ae. aegypti individuals than the refractory mosquitoes. The functional annotation of ESTs revealed a broad response in the susceptible library that included immune molecules, metabolic molecules and transcription factors. In the refractory strain, there was the presence of a trypsin inhibitor gene, which could play a role in the infection. These results serve as a template for more detailed studies aiming to characterize the genetic components of refractoriness, which in turn can be used to devise new approaches to combat transmission of dengue fever.
authors.affiliation: Centro lnternacional de Entrenamiento e Investigaciones Médicas, Cali, Colombia.
authors.forename: Olga L
authors.initials: OL
authors.lastname: Barón
authors.forename: Raul J
authors.initials: RJ
authors.lastname: Ursic-Bedoya
authors.forename: Carl A
authors.initials: CA
authors.lastname: Lowenberger
authors.forename: Clara B
authors.initials: CB
authors.lastname: Ocampo
mesh_terms: D000330:Aedes / Q000378:metabolism* / Q000821:virology*; D000818:Animals; D003716:Dengue Virus / Q000502:physiology*; D004064:Digestive System / Q000378:metabolism*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D015723:Gene Library