Genome-wide gene expression changes in genetically stable synthetic and natural wheat allohexaploids.
*The present study aims to understand regulation of gene expression in synthetic and natural wheat (Triticum aestivum) allohexaploids, that combines the AB genome of Triticum turgidum and the D genome of Aegilops tauschii; and which we have recently characterized as genetically stable. *We conducted a comprehensive genome-wide analysis of gene expression that allowed characterization of the effect of variability of the D genome progenitor, the intergenerational stability as well as the comparison with natural wheat allohexaploid. We used the Affymetrix GeneChip Wheat Genome Array, on which 55 049 transcripts are represented. *Additive expression was shown to represent the majority of expression regulation in the synthetic allohexaploids, where expression for more than c. 93% of transcripts was equal to the mid-parent value measured from a mixture of parental RNA. This leaves c. 2000 (c. 7%) transcripts, in which expression was nonadditive. No global gene expression bias or dominance towards any of the progenitor genomes was observed whereas high intergenerational stability and low effect of the D genome progenitor variability were revealed. *Our study suggests that gene expression regulation in wheat allohexaploids is established early upon allohexaploidization and highly conserved over generations, as demonstrated by the high similarity of expression with natural wheat allohexaploids.
Chagué
Véronique
V
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Just
Jérémy
J
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Mestiri
Imen
I
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Balzergue
Sandrine
S
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Tanguy
Anne-Marie
AM
Unité Mixte de Recherches INRA - Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F-35653 Le Rheu, France.
Huneau
Cecile
C
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Huteau
Virginie
V
Unité Mixte de Recherches INRA - Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F-35653 Le Rheu, France.
Belcram
Harry
H
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
Coriton
Olivier
O
Unité Mixte de Recherches INRA - Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F-35653 Le Rheu, France.
Jahier
Joseph
J
Unité Mixte de Recherches INRA - Agrocampus Rennes, Amélioration des Plantes & Biotechnologies Végétales, F-35653 Le Rheu, France.
Chalhoub
Boulos
B
Organization and Evolution of Plant Genomes (OEPG), Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - CNRS 8114 - UEVE, F-91057 Evry Cedex, France.
D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D006109:Poaceae / Q000235:genetics*; D011123:Polyploidy; D018749:RNA, Plant; D014908:Triticum / Q000235:genetics*
D018749:RNA, Plant