Anti-allergic potential of oligomannose-coated liposome-entrapped Cry j 1 as immunotherapy for Japanese cedar pollinosis in mice.
Administration of oligomannose-coated liposomes (OMLs) in mice can induce Th1 immune responses against antigens entrapped in the OMLs. In the present study, we investigated the anti-allergic effect of treatment with oligomannose-coated liposomes (OMLs) with entrapped Cry j 1, a major allergen of Japanese cedar pollen (Cry j 1/OMLs), in Balb/c mice sensitized with Cry j 1. Pretreatment of unsensitized mice with Cry j 1/OMLs repressed the elevation of total and allergen-specific IgE levels in sera elicited in response to subsequent sensitization with Cry j 1. Cry j 1-specific IgG1 in sera also decreased in Cry j 1/OML-treated mice, while the levels of Cry j 1-specific IgG2a in these mice significantly increased after sensitization with Cry j 1. In addition, Cry j 1/OML-treated mice showed high IFN-gamma production from spleen cells and low IL-4/IFN-gamma and IL-5/IFN-gamma ratios in response to in vitro stimulation with Cry j 1. These results indicate that allergen-specific Th1 immune responses predominate over Th2 responses and control IgE elevation in Cry j 1/OML-treated mice. The anti-allergic effect of Cry j 1/OML determined by suppression of serum IgE levels was also observed in Cry j 1-presensitized mice after challenge with antigen. Thus, Cry j 1/OMLs may be useful for immunotherapeutic control of allergic reactions to Japanese cedar pollinosis.
Ishii
Mariko
M
Institute of Glycoscience, Tokai University, Hiratsuka, Kanagawa 259-1292, Japan.
Koyama
Aki
A
Iseki
Hiroshi
H
Narumi
Hideki
H
Yokoyama
Naoaki
N
Kojima
Naoya
N
D000485:Allergens / Q000627:therapeutic use; D000818:Animals; D052179:Antigens, Plant / Q000008:administration & dosage*; D032485:Cryptomeria / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D007372:Interferons / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D015848:Interleukin-5 / Q000097:blood / Q000276:immunology; D008081:Liposomes; D008358:Mannose / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000008:administration & dosage*; D010940:Plant Proteins / Q000008:administration & dosage*; D006255:Rhinitis, Allergic, Seasonal / Q000628:therapy*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D007074:Immunoglobulin G; D015848:Interleukin-5; D008081:Liposomes; D009844:Oligosaccharides; D010940:Plant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E; D007372:Interferons; D008358:Mannose