title: Activities of enzymes that hydrolyze adenine nucleotides in platelets from patients with rheumatoid arthritis.
abstract: OBJECTIVE
  To investigate whether there are changes in the activity of the enzymes NTPDase, 5'-nucleotidase, E-NPP and ADA in platelets from patients with rheumatoid arthritis (RA).
  
  
  METHODS
  Thirty-five RA patients diagnosed with RA through American College of Rheumatology criteria, as well as 35 healthy patients were selected. NTPDase, 5'-nucleotidase, E-NPP and ADA activities were verified in platelets isolated from these patients.
  
  
  RESULTS
  The results demonstrate that an increase in NTPDase (approximately 100%), 5'-nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) activities occurred in RA patients when compared to the control group.
  
  
  CONCLUSIONS
  Ours results suggest an increase in the NTPDase, 5'-nucleotidase and E-NPP activities, which could be related to a compensatory organic response to excessive platelet aggregation which occurs during the inflammation. The increased ADA activity found in this work could lead to a decrease in the adenosine concentration in the circulation, which could explain the accelerated atherosclerosis found in patients with RA.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av Roraima, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Lara V
authors.initials: LV
authors.lastname: Becker
authors.forename: Cintia S
authors.initials: CS
authors.lastname: Rosa
authors.forename: Viviane do C G
authors.initials: Vdo C
authors.lastname: Souza
authors.forename: Margarete D
authors.initials: MD
authors.lastname: Bagatini
authors.forename: Emerson A
authors.initials: EA
authors.lastname: Casali
authors.forename: Claudio Alberto M
authors.initials: CA
authors.lastname: Leal
authors.forename: João Carlos N
authors.initials: JC
authors.lastname: da Silva
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Moretto
authors.forename: Francielle de V
authors.initials: Fde V
authors.lastname: Pinheiro
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Daniela B R
authors.initials: DB
authors.lastname: Leal
mesh_terms: D015720:5'-Nucleotidase; D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D050197:Atherosclerosis / Q000209:etiology; D001792:Blood Platelets / Q000201:enzymology* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases
chemical_list: D000227:Adenine Nucleotides; D006867:Hydrolases; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D000243:Adenosine Deaminase; D011755:Pyrophosphatases