title: [Forward genetic screening for zebrafish mutants defective in myelopoiesis].
abstract: OBJECTIVE
  To identify zebrafish mutants with myelopoiesis defects by ENU mutagenesis and large-scale forward genetic screening.
  
  
  METHODS
  Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in the spermatogonial cells to generate the founders, which were outcrossed with AB to raise F1 fish. The F1 fish from different founders were mated to generate the F2 families. The F3 embryos from F2 sibling crosses were screened by Sudan black B staining and neutral red staining.
  
  
  RESULTS
  A total of 350 F2 families from F1 sibling crosses were screened, and 1424 F2 crosses were analyzed. Six mutations were identified resulting in abnormal Sudan black B staining and neutral red staining, indicating the involvement of neutrophil deficiency or macrophage abnormalities.
  
  
  CONCLUSIONS
  It is simple and cheap to induce and screen myelopoiesis deficiency in zebrafish by ENU chemical mutagenesis and Sudan black B staining and neutral red staining. These mutants shed light on the identification of the genes important to myelopoiesis in zebrafish.
authors.affiliation: Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. daizhaoxia@yahoo.cn
authors.forename: Zhao-xia
authors.initials: ZX
authors.lastname: Dai
authors.forename: Guang
authors.initials: G
authors.lastname: Yan
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zhong-jun
authors.initials: ZJ
authors.lastname: Huo
authors.forename: Zong-hua
authors.initials: ZH
authors.lastname: Wen
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Zhi-bing
authors.initials: ZB
authors.lastname: Huang
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Ping-yun
authors.initials: PY
authors.lastname: Ma
authors.forename: Wei-hao
authors.initials: WH
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shu
authors.initials: S
authors.lastname: Fan
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Huang
authors.forename: Zi-long
authors.initials: ZL
authors.lastname: Wen
authors.forename: Wen-qing
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005820:Genetic Testing; D008297:Male; D016296:Mutagenesis; D009154:Mutation; D023461:Myeloid Progenitor Cells / Q000502:physiology*; D038042:Myelopoiesis / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*