Dendritic cells loaded with tumor B cells elicit broad immunity against murine gammaherpesvirus 68 but fail to prevent long-term latency.
It is still unknown whether a noninfectious gammaherpesvirus vaccine is able to prevent or reduce virus persistence. This led us to use dendritic cells loaded with tumor B cells as a vaccine approach for the murine gammaherpesvirus 68 (gammaHV68) model of infection. Dendritic cells loaded with UV-irradiated latently infected tumor B cells induce broad, strong, and long-lasting immunity against gammaHV68. Dendritic cell vaccination prevents the enlargement of lymph nodes and severely limits acute infection and early latency but does not prevent gammaHV68 from establishing long-term latency. Our findings support the concept that attenuated viruses may be the best vaccine option for preventing gammaherpesvirus persistence.
Weslow-Schmidt
Janet
J
Center for Vaccines and Immunity, Research Institute at Nationwide Children's Hospital, Columbus, Ohio 43205, USA.
Ye
Fang
F
Cush
Stephanie S
SS
Stuller
Kathleen A
KA
Blackman
Marcia A
MA
Flaño
Emilio
E
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D018142:Gammaherpesvirinae / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006566:Herpesviridae Infections / Q000276:immunology* / Q000821:virology; D006801:Humans; D016393:Lymphoma, B-Cell / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D017735:Virus Latency
D019496:Cancer Vaccines