Optical DNA detection based on gold nanorods aggregation.
Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Herein, the localized surface plasmon resonance properties of unmodified gold nanorods (GNRs) in 1 mM cetyltrimethyl-ammonium bromide solution were used for sensing DNA sequences, with good simplicity and sensitivity. The intensity of typical plasmon resonance absorption bands of the GNRs decreased with increasing cDNA concentration. The detection of a 30-mer single-stranded oligonucleotide as a model reached a detection limit of about 0.1 pM. This study will be significant for as-prepared GNRs for future application in biological systems.
Ma
Zhanfang
Z
Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@yahoo.com
Tian
Le
L
Wang
Tingting
T
Wang
Chungang
C
D004247:DNA / Q000032:analysis*; D020869:Gene Expression Profiling; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance / Q000379:methods*
D006046:Gold; D004247:DNA