Molecular characterization of human group A rotavirus from stool samples in young children with diarrhea in Indonesia.
Detection and genotyping of group A rotavirus strains from stool samples in young children with diarrhea in Indonesia were examined using reverse transcription-nested multiplex PCR. Of 421 stool specimens, 257 samples was rotavirus positive. G1 type was the most common G-type (54%), followed by G2 (6%) and G9 (3%). P[8] was the most common P-type (39%), followed by P[6] (19%), P[4] (10%) and P[11] 1%. Eighteen percent of the samples had mixed G genotype infection and 5% had mixed P genotype infection. The prevalence of G-P combination type was genotype G1P[8] (24%), followed by G1P[6] (7%), G2P[4] (3%), and G1P[4] (2%). A total of 118 specimens could not be assigned as a G and/or P type suggesting the presence of new circulating genotypes in Indonesia.
Radji
Maksum
M
Laboratory of Microbiology and Biotechnology, Department of Pharmacy, Faculty of Mathematics and Sciences, University of Indonesia, Depok, Indonesia. maksum@farmasi.ui.ac.id
Putman
Shannon D
SD
Malik
Amarila
A
Husrima
Refda
R
Listyaningsih
Erlin
E
D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D007223:Infant; D008297:Male; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*