title: Direct determination of urinary lysozyme using surface plasmon resonance light-scattering of gold nanoparticles.
abstract: The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.
authors.affiliation: College of Sciences, Shenyang Agricultural University, Shenyang, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.forename: Ke
authors.initials: K
authors.lastname: Hu
authors.forename: Minghui
authors.initials: M
authors.lastname: Xiang
authors.forename: Limei
authors.initials: L
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D006046:Gold / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000652:urine*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D006046:Gold; D009113:Muramidase