title: Modulation of the striato-pallidal pathway by adenosine A2a receptors depends on dopaminergic striatal input.
abstract: The modulation of the striato-pallidal pathway by presynaptic adenosine A2a and dopamine D2 receptors has gained attention in the study of Parkinson's disease. Here, we analyzed the effect of presynaptic A2a receptors in the spiking activity of globus pallidus (GP) neurons recorded during electrical stimulation of the striato-pallidal pathway, in both sham and ipsilaterally dopamine-denervated rats. We found that intrapallidal blockade of A2a by 100 pMol KF-17383 in sham and lesioned rats did not modify the spiking rate of GP neurons. Local infusion of 100 pMol CGS-21680, an A2a agonist, did not change the spiking rate in sham rats, whereas the same concentration of NMDA strongly increased the firing frequency of all neurons tested. Moreover, in sham rats, local blockade of A2a receptors by 100 pMol KF-17383 suppressed the inhibition evoked by activation of the striato-pallidal pathway, while in dopamine-denervated rats the same dose of KF-17383 did not modify the inhibition. Our results show that the contribution of A2a receptors to the spiking control of GP by the striato-pallidal pathway depends on the state of the dopaminergic system.
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, México D.F., México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Querejeta
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez-Romero
authors.forename: Joan Erick-Gómez
authors.initials: JE
authors.lastname: Miranda
authors.forename: Alfonso
authors.initials: A
authors.lastname: Delgado
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000241:Adenosine / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005917:Globus Pallidus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008474:Medial Forebrain Bundle / Q000187:drug effects / Q000502:physiology; D016202:N-Methylaspartate / Q000494:pharmacology; D009434:Neural Pathways / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D043683:Receptors, Adenosine A2 / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D018690:Excitatory Amino Acid Agonists; D043683:Receptors, Adenosine A2; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D000241:Adenosine; D004298:Dopamine