Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using Tris-based extenders.
The objective was to evaluate the influence of the thawing rate on the quality of frozen-thawed (cryopreserved in Tris-based extenders) semen obtained from collared peccaries (Tayassu tajacu). Semen from 13 sexually mature collared peccaries males were collected by electroejaculation, and evaluated for motility, vigor, sperm viability, membrane integrity, and sperm morphology. Semen was divided in two equal portions: the first was diluted in Tris-fructose and the other in Tris-glucose, with egg yolk (20%) and glycerol (3%) added to each portion. Extended semen was frozen in liquid nitrogen and thawed using two thawing protocols (37 degrees C for 1 min or 55 degrees C for 7 s, followed by an additional 30 s at 37 degrees C). There were no significant differences between the two extenders after extension, chilling, or glycerol addition. After thawing at 37 degrees C, there were 37.9 +/- 4.2% and 28.5 +/- 5.1% motile spermatozoa for samples extended in Tris-fructose and Tris-glucose, respectively, with 33.8 +/- 3.7% and 28.2 +/- 3.5% motile spermatozoa after thawing at 55 degrees C (no significant differences). Furthermore, there were no significant interactions between extenders and thawing protocols for any semen end point. In conclusion, semen from collared peccaries was successfully cryopreserved in Tris-based extenders and thawed with two protocols (37 degrees C for 1 min or 55 degrees C for 7 s).
Castelo
T S
TS
Departamento de Ciências Animais, Universidade Federal Rural do Semi-Arido, UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoro, RN, Brazil.
Bezerra
F S B
FS
Souza
A L P
AL
Moreira
M A P
MA
Paula
V V
VV
Oliveira
M F
MF
Silva
A R
AR
D000818:Animals; D001188:Artiodactyla / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D005615:Freezing / Q000009:adverse effects; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D012661:Semen / Q000187:drug effects / Q000502:physiology; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013696:Temperature; D014325:Tromethamine / Q000494:pharmacology*
D003451:Cryoprotective Agents; D014325:Tromethamine; D005990:Glycerol