Association of anti-triosephosphate isomerase antibody and MRL/MpJ-Faslpr mouse.
The association between anti-triosephosphate isomerase (TPI) antibodies and MRL/MpJ-Fas(lpr) (MRL/lpr) mice was examined. We found that serum anti-TPI antibody levels in MRL/lpr mice, measured by enzyme-linked immunosorbent assay, were significantly higher than that of age-matched Balb/c mice and NZB/WF1 mice. Anti-TPI antibodies were detected in serum and cerebrospinal fluid in MRL/lpr mice by Western blotting. Inoculation of anti-TPI monoclonal antibody-producing hybridoma into the brain of Balb/c mice resulted in immunoglobulin deposition in the regions near the ventricles, hippocampus, and choroid plexus. Anti-TPI antibodies may play a role in the etiology of brain damage and behavioral deficits in MRL/lpr mice.
Sato
Shuzo
S
Fukushima Medical University, Department of Gastroenterology and Rheumatology, School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.
Watanabe
Hiroshi
H
Shio
Kiori
K
Kobayashi
Hiroko
H
Ohira
Hiromasa
H
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood* / Q000134:cerebrospinal fluid; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D019463:Mice, Inbred MRL lpr / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology*; D014305:Triose-Phosphate Isomerase / Q000276:immunology* / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D014305:Triose-Phosphate Isomerase