Identification of lipid droplet-associated proteins in the formation of macrophage-derived foam cells using microarrays.
A large number of macrophage-derived foam cells stores excessive neutral lipids in intracellular droplets, and plays a major role during the development of atherosclerosis. The formation and catabolism of intracellular lipid droplets (LDs) are regulated by LD-associated proteins, a group of proteins which are located on the surface of LDs and regulate the formation, morphology and lipolysis of LDs. In order to illustrate the function of LD-associated proteins during the process of atherosclerosis, the foam cell model is induced by oxidized low-density lipoprotein (ox-LDL) in macrophages originated from the THP-1 cell line, and cDNA microarrays are used to monitor the gene expression profiles of LD-associated proteins. Gene expression data show that 2% of changed genes are lipid binding genes during the transformation of foam cells. The major candidate genes, the cell death-inducing DFF45-like effector (CIDE) family and Perilipin, Adipophilin, and TIP47 (PAT) family, have different alterations during the formation of foam cells. CIDEB, CIDEC, Adipophilin, S3-12 and LSDP5 were up-regulated, while TIP47 was down-regulated. There was no significant change in CIDEA and Perilipin. These results were confirmed by real-time PCR and immunoblotting. This study presents a comprehensive analysis of the gene expression of LD-associated proteins during the differentiation of human foam cells, which may play an important role in the process of atherosclerosis.
Li
Hang
H
State Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an, P.R. China.
Song
Yue
Y
Li
Fanfan
F
Zhang
Lijun
L
Gu
Yu
Y
Zhang
Liying
L
Jiang
Lina
L
Dong
Wenpeng
W
Ye
Jing
J
Li
Qing
Q
D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D005487:Foam Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D050356:Lipid Metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D015703:Antigens, CD; D011506:Proteins