title: Beneficial effects of fructo-oligosaccharides supplementation on fecal bifidobacteria and index of peroxidation status in constipated nursing-home residents--a placebo-controlled, diet-controlled trial.
abstract: OBJECTIVE
  This study assessed effects of fructo-oligosaccharides (FOS) supplementation on fecal bifidobacteria, lipid peroxidation index, indices of nutritional status, and whether effects of FOS were sustained after its withdrawal in constipated nursing-home residents. The associations of fecal bifidobacteria and blood measurements were also examined.
  
  
  METHODS
  Six men and four women participated in a double-blind, diet-controlled study that consisted of a 4-wk placebo (3 mL of fructose syrup) period, a 4-wk FOS (10 g/d) period, and a 4-wk post-FOS period. Stools were collected during the last week of each period to determine the microflora and fecal weight. Fasting blood was collected at the end of each period and analyzed for thiobarbituric acid-reactive substances (TBARS) and biochemical indices.
  
  
  RESULTS
  Fecal counts (log counts/gram of dry feces) and daily fecal output of bifidobacteria significantly increased with FOS compared with placebo. The effect on bifidobacteria output was sustained in the post-FOS period. Plasma TBARS concentration was reduced by 16% and 21% in the FOS and post-FOS periods, respectively, compared with that in the placebo period. The plasma cholesterol level was significantly lowered by 7% in the FOS and post-FOS periods compared with that in the placebo period. The increases in fecal bifidobacteria output during the FOS period (log colony-forming units per day) were associated with decreases in plasma TBARS and plasma cholesterol, respectively.
  
  
  CONCLUSIONS
  Supplementation of FOS increases the daily output of bifidobacteria and decreases plasma TBARS and cholesterol concentrations in constipated nursing-home elderly residents and these effects remained at the end the post-FOS period.
authors.affiliation: Department of Family and Community Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Chi-Hua
authors.initials: CH
authors.lastname: Yen
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Kuo
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Tseng
authors.forename: Meng-Chih
authors.initials: MC
authors.lastname: Lee
authors.forename: Hsiao-Ling
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000368:Aged; D001644:Bifidobacterium / Q000187:drug effects*; D002784:Cholesterol / Q000097:blood; D003248:Constipation / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D003672:Defecation / Q000187:drug effects*; D019587:Dietary Supplements; D004311:Double-Blind Method; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009735:Nursing Homes; D009844:Oligosaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D056692:Prebiotics; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; D017392:Thiobarbituric Acid Reactive Substances; D002784:Cholesterol