Granulocyte antibody screening: evaluation of a bead-based assay in comparison with classical methods.
BACKGROUND
Granulocyte antibodies have been implicated in allo- and autoimmune neutropenia and in transfusion reactions.


METHODS
Fifty-one sera from suspected alloimmune neutropenia or transfusion-related acute lung injury (TRALI) and 40 sera from suspected autoimmune neutropenia were tested for granulocyte antibodies using LABScreen MULTI (One Lambda, Inc.), compared with classical tests (flow cytometry [FC] and granulocyte agglutination [GAT] followed by monoclonal antibody-specific immobilization of granulocyte antigens [MAIGA]).


RESULTS
In alloimmune situations, 48 sera were concordant (94%), two sera positive for HNA with LABScreen MULTI were negative by FC/GAT and/or MAIGA, and one serum sample negative for HNA with LABScreen MULTI was positive by classical tests. In autoimmune neutropenia, 30 sera were concordant (75%), four sera positive for HNA with LABScreen MULTI were negative by FC/GAT and/or MAIGA, and six sera negative for HNA with LABScreen MULTI were positive by FC/GAT and/or MAIGA. For detection of autoantibodies, the LABScreen MULTI was less concordant. However, with the exception of one case, the discrepancies were observed in sera that did not show a clear specificity.


CONCLUSIONS
LABScreen MULTI correlated well with our classical methods for HNA-1 and HNA-2a antibody screening. It can be used for screening blood donors or patients suspected of TRALI, but GAT is still needed for HNA-3a antibody screening.
Fromont
Patricia
P
Laboratoire d'Immunologie, CHU Nantes, France.
Prié
Nolwen
N
Simon
Philippe
P
Cesbron-Gautier
Anne
A
Quelvennec
Erwann
E
Bignon
Jean-Denis
JD
Muller
Jean-Yves
JY
Audrain
Marie
M
D055371:Acute Lung Injury / Q000097:blood / Q000209:etiology / Q000276:immunology; D000906:Antibodies / Q000032:analysis* / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D018189:Immunomagnetic Separation / Q000379:methods* / Q000592:standards; D007231:Infant, Newborn; D007519:Isoantigens / Q000032:analysis / Q000276:immunology; D008403:Mass Screening / Q000379:methods* / Q000592:standards; D009503:Neutropenia / Q000097:blood / Q000151:congenital / Q000276:immunology; D012016:Reference Values; D012698:Serologic Tests / Q000379:methods / Q000592:standards; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000276:immunology; D065227:Transfusion Reaction
D000906:Antibodies; D001323:Autoantibodies; D007519:Isoantigens