Rapid enumeration of respiratory active mycobacteria with fluorescent double staining.
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach.
Ichijo
Tomoaki
T
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Izumi
Yoko
Y
Yamaguchi
Nobuyasu
N
Nasu
Masao
M
D005456:Fluorescent Dyes / Q000737:chemistry; D009161:Mycobacterium / Q000737:chemistry* / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D010100:Oxygen