title: Toxicopathological effects of lambda-cyhalothrin in female rabbits (Oryctolagus cuniculus).
abstract: The aim of this study was to investigate effects of lambda-cyhalothrin (LCT) on clinical, hematological, biochemical and pathological alterations in rabbits (Oryctolagus cuniculus). New Zealand white female rabbits (n = 24) of 4-5 months age having 997.92 ± 32.83 g weight were divided into four equal groups. Group A (control) received normal saline intraperitoneally (ip). Animals in groups B, C and D were treated with LCT 1.0, 4.0 and 8.0 mg/kg bw ip. Each group received seven consecutive doses at an interval of 48 hours. Blood and serum samples were collected at an interval of 96 hours. Blood analysis revealed a significant (p < 0.05) decrease in red blood cell and white blood cell counts, hemoglobin concentration and lymphocytes, while mean corpuscular hemoglobin concentration, mean corpuscular volume, neutrophils, monocytes and eosinophils were increased. Serum biochemical analysis revealed significant (p < 0.05) decrease in serum total proteins and serum albumin, while an increase was seen in serum alanine aminotransferase and aspartate aminotransferase activities compared with the control group. Serum globulin values varied non-significantly in all treatment groups as compared to control group. A dose-dependent increase in the incidence of micronucleated polychromatic erythrocyte was observed. All gross and histopathological lesions observed in LCT-treated rabbits were dose-dependent. Liver of the treated rabbits exhibited extensive perihepatitis, hyperplasia of bile duct, necrosis, hemorrhages and congestion. In lungs, there were hemorrhages, thickened alveolar walls, congestion, emphysema, collapsed alveoli and accumulation of extensive inflammatory cells. Kidneys were congested and hemorrhagic whereas renal parenchyma and stroma were normal. Microscopically, heart showed congestion of blood vessels and nuclear pyknosis, myodegeneration. It was concluded from the study that LCT produced toxicopathological alterations in rabbits in a dose-dependent manner. On the basis of the results, it can be suggested that overdosing of LCT be avoided while treating animals for ectoparasites.
authors.affiliation: Department of Pathology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Basir
authors.forename: Ahrar
authors.initials: A
authors.lastname: Khan
authors.forename: Riaz
authors.initials: R
authors.lastname: Mustafa
authors.forename: Muhammad Zargham
authors.initials: MZ
authors.lastname: Khan
authors.forename: Farzana
authors.initials: F
authors.lastname: Rizvi
authors.forename: Fazal
authors.initials: F
authors.lastname: Mahmood
authors.forename: Arfan
authors.initials: A
authors.lastname: Yousaf
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D019963:Clinical Chemistry Tests; D004249:DNA Damage; D005260:Female; D006403:Hematologic Tests; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology; D007274:Injections, Intraperitoneal; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009336:Necrosis / Q000139:chemically induced; D009570:Nitriles / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D011817:Rabbits
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C037304:cyhalothrin