Discrete and active enzyme nanoarrays on DNA origami scaffolds purified by affinity tag separation.
Desired enzyme nanoarrays patterned on a DNA origami scaffold were selectively isolated by affinity tag purification from a pool of differently patterned nanoarrays, and their enzymatic activity was successfully confirmed. As few as 12 histidine residues were enough to hold a huge complex of DNA origami with multiple proteins, 260 nm in length and 5.2 MDa in molecular weight, to an immobilized metal affinity resin.
Numajiri
Kentaro
K
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Yamazaki
Takahiro
T
Kimura
Mayumi
M
Kuzuya
Akinori
A
Komiyama
Makoto
M
D000345:Affinity Labels / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D036103:Nanotechnology / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*
D000345:Affinity Labels; D004798:Enzymes; D004247:DNA