title: Analyzing binding data.
abstract: Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments.
authors.affiliation: GraphPad Software, La Jolla, California, USA.
authors.forename: Harvey J
authors.initials: HJ
authors.lastname: Motulsky
authors.forename: Richard R
authors.initials: RR
authors.lastname: Neubig
mesh_terms: D001667:Binding, Competitive; D005453:Fluorescence; D005454:Fluorescence Polarization / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D007700:Kinetics; D008024:Ligands; D016014:Linear Models; D008956:Models, Chemical; D017711:Nonlinear Dynamics; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000737:chemistry; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D008024:Ligands; D019275:Radiopharmaceuticals; D043562:Receptors, G-Protein-Coupled