title: Thermal analysis reveals differential effects of various crosslinkers on bovine annulus fibrosis.
abstract: Treatment of a pathological spinal disc in vivo by injection of protein crosslinking reagents to restore the disc's mechanical properties is a new approach to the treatment of degenerative disc disease. In this study, the thermal stability of the collagen in disc annulus was measured by differential scanning calorimetry following treatment with six different crosslinking agents. The crosslinkers used were; L-threose (LT), genipin (GP), methylglyoxal (MG), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), glutaraldehyde (GA), and proanthrocyanidin (PA). Untreated tissue displayed a prominent peak at about 66-68°C. Comparison of endothermal patterns of untreated and crosslinker-treated disc annulus tissue samples showed that a new peak appeared at a higher temperature following treatment. The temperature of the new peak qualitatively depended on the crosslinker in the following order GA > MG > GP > PA = EDC > LT, suggesting that the enhanced thermal stability of collagen in the annulus tissue was related to the nature of the crosslinker. Also, the enthalpic ratios of the lower temperature (noncrosslinked) peaks in the treated and untreated tissue, and of the higher and lower temperature peaks in the treated tissue, both indicated that the various agents crosslinked the tissue with different efficiencies. Our data suggest that the ability of GP to penetrate into the disc and form long- and short-range crosslinks may make it the most suitable candidate for clinical development. In addition, binary combinations of long- and short-range crosslinkers, such as PA with LT, may also provide synergistic effects due to their substantially different physicochemical properties.
authors.affiliation: Orthopeutics, LP, 111 Cooperative Way, Suite 210, Georgetown, Texas 78626, USA. kzhu@orthopeutics.com
authors.forename: Keng
authors.initials: K
authors.lastname: Zhu
authors.forename: Paul
authors.initials: P
authors.lastname: Slusarewicz
authors.forename: Tom
authors.initials: T
authors.lastname: Hedman
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005355:Fibrosis; D007403:Intervertebral Disc / Q000473:pathology*; D009994:Osmolar Concentration; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D003094:Collagen