title: Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells.
abstract: Mammalian tissue cultured cells are widely used in cell biology research. Immunoelectron microscopy is a powerful technique to define the subcellular localization of targeted antigens in the cultured cells. Cryofixation is now generally accepted as the best initial fixation step, to preserve the cellular fine structures and antigenicity. This chapter covers the practical procedures for immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue cultured cells processed by high-pressure freezing.
authors.affiliation: Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Sawaguchi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D017110:Freeze Substitution / Q000379:methods*; D005615:Freezing; D017979:Gold Colloid / Q000737:chemistry; D016253:Microscopy, Immunoelectron / Q000379:methods*; D011312:Pressure; D011817:Rabbits
chemical_list: D000906:Antibodies; D017979:Gold Colloid