Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.
In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.
Yamamoto
Mai
M
Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8602, Japan.
Morita
Ryoko
R
Mizoguchi
Takamasa
T
Matsuo
Hiromi
H
Isoda
Miho
M
Ishitani
Tohru
T
Chitnis
Ajay B
AB
Matsumoto
Kunihiro
K
Crump
J Gage
JG
Hozumi
Katsuto
K
Yonemura
Shigenobu
S
Kawakami
Koichi
K
Itoh
Motoyuki
M
D016475:3T3 Cells; D000818:Animals; D019521:Body Patterning; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D019070:Cell Lineage; D002522:Chlorocebus aethiops; D004705:Endocytosis; D018507:Gene Expression Regulation, Developmental; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D009672:Notochord / Q000502:physiology*; D051880:Receptors, Notch / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D025801:Ubiquitin; D029961:Zebrafish Proteins; C000606276:jag1a protein, zebrafish; D044767:Ubiquitin-Protein Ligases; C471932:mib1 protein, zebrafish