title: Antimicrobial susceptibility profiles of meticillin-susceptible and -resistant Staphylococcus aureus: focus on daptomycin minimum inhibitory concentrations at a tertiary care centre in Mumbai, India.
abstract: In this study, daptomycin minimum inhibitory concentrations (MICs) for Staphylococcus aureus isolates were determined using Etest strips and were correlated with staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene positivity. In total, 60 meticillin-resistant S. aureus (MRSA) and 60 meticillin-susceptible S. aureus (MSSA) isolates from clinical samples were subjected to antimicrobial susceptibility testing by disk diffusion according to Clinical and Laboratory Standards Institute guidelines, polymerase chain reaction (PCR) detection of PVL and mecA genes, and SCCmec typing. Daptomycin MICs were determined using Etest strips. The mecA gene was present in all MRSA isolates and was absent in 59 MSSA isolates. The PVL gene was present in 41 MRSA isolates and 25 MSSA isolates. Amongst the MRSA isolates, 10 were SCCmec type III, 27 were SCCmec IV and 23 were SCCmec V. Moreover, 26 SCCmec IV and 15 SCCmec V isolates were PVL-positive. All SCCmec III isolates were multidrug-resistant and PVL-negative. Daptomycin MICs ranged from 0.047 microg/mL to 1 microg/mL for MRSA and from 0.19 microg/mL to 1 microg/mL for MSSA. All MRSA and MSSA isolates were susceptible to daptomycin. Although SCCmec III MRSA and PVL-positive MSSA were resistant to more antimicrobial classes than SCCmec IV and V MRSA and PVL-negative MSSA, there does not appear to be a significant correlation between SCCmec types, PVL positivity and daptomycin MICs. MICs were not as low as expected for a newly introduced drug.
authors.affiliation: Research Laboratories, P.D. Hinduja National Hospital & Medical Research Centre, Mahim, Mumbai 400016, India.
authors.forename: Namita
authors.initials: N
authors.lastname: D'Souza
authors.forename: Anjali
authors.initials: A
authors.lastname: Shetty
authors.forename: Ajita
authors.initials: A
authors.lastname: Mehta
authors.forename: Camilla
authors.initials: C
authors.lastname: Rodrigues
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D017576:Daptomycin / Q000494:pharmacology*; D005098:Exotoxins / Q000235:genetics; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007194:India; D007956:Leukocidins / Q000235:genetics; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; D037521:Virulence Factors; C487789:mecA protein, Staphylococcus aureus; D017576:Daptomycin