title: Infection with specific Helicobacter pylori-cag pathogenicity island strains is associated with interleukin-1B gene polymorphisms in Venezuelan chronic gastritis patients.
abstract: BACKGROUND
  The cag pathogenicity island (cag-PAI) is one of the major virulence factors of Helicobacter pylori, showing considerable geographic variation.
  
  
  OBJECTIVE
  We investigated the prevalence of cagA, cagE, and cagT genes of cag-PAI and their association with proinflammatory IL-1B-511/-31/+3954 polymorphisms in Venezuelan chronic gastritis patients from a high-risk gastric cancer region.
  
  
  METHODS
  Presence of cag-PAI genes and IL-1B polymorphisms in 121 biopsy specimens was evaluated by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP), respectively.
  
  
  RESULTS
  cagA (+) and triple-positive (cagAET (+)) strains were detected in 79.3% and 70.2% of patients, respectively. We found that infection with cagA (+) and cagAET (+) strains was associated (P < 0.05) with hosts harboring both IL-1B +3954C allele and IL-1B-511T/-31C/+3954C haplotype (TCC (+)). The frequency of gastric atrophy was significantly higher (P < 0.020) among cagAET (+)/IL-1B-TCC (+) combined genotype carriers.
  
  
  CONCLUSIONS
  Carriage of IL-1B +3954C allele and IL-1B-TCC (+) haplotype could favor colonization of bacterial cagAET (+) strains, and the combination of these bacterial and host haplotypes could play a synergistic role in development of premalignant gastric lesions. This work contributes to understanding of the complex interaction between H. pylori virulence factors and cytokine genotypes involved in gastrointestinal diseases.
authors.affiliation: Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela. mchiurillo@ucla.edu.ve
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Chiurillo
authors.forename: Yeinmy H
authors.initials: YH
authors.lastname: Moran
authors.forename: Miryan
authors.initials: M
authors.lastname: Cañas
authors.forename: Elvis J
authors.initials: EJ
authors.lastname: Valderrama
authors.forename: Emma
authors.initials: E
authors.lastname: Armanie
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002908:Chronic Disease; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D020022:Genetic Predisposition to Disease; D016480:Helicobacter pylori / Q000145:classification* / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014687:Venezuela / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D053583:Interleukin-1beta; C080866:cagA protein, Helicobacter pylori