Patterned PDMS based cell array system: a novel method for fast cell array fabrication.
Cell-cell interaction is important in numerous biological processes, including cell growth, cell differentiation and migration. The ability to generate pre-determined cell patterns or cell arrays on a study surface is crucial for cell-cell interaction studies. In this paper, we developed a method for fast cell array fabrication using laser sintering and the hydrophobicity of PDMS films. This approach can be easily adopted and is cost-effective. Hydrophobic PDMS films were fabricated into polymeric chips containing hundreds of microwells. The films were then transferred onto tissue culture surfaces to separate cells in the formation of cell arrays (Patterned PDMS based cell array system, PCAS). We used NIH/3T3 fibroblast cells to demonstrate the feasibility of PCAS. The success of fast fabrication of patterned cell arrays was obtained using different initial cell seeding densities. We also used poorly adherent PC-12 cells to demonstrate the cell-cell communication. Results showed that the method is very useful for studying topics such as cell-cell interaction, cell-substrate interaction or cell migration.
Hsieh
Chin-Hsiung
CH
Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, No. 1, Jen-Ai Road, Section 1, Taipei, Taiwan.
Huang
Chi-Jer Charles
CJ
Huang
Yi-You
YY
D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007834:Lasers; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors; D046888:Tissue Array Analysis / Q000379:methods*
D004129:Dimethylpolysiloxanes; C013830:baysilon