title: Effects of Cissampelos sympodialis Eichl. and its alkaloid, warifteine, in an experimental model of respiratory allergy to Blomia tropicalis.
abstract: Asthma is one of the most prevalent chronic diseases worldwide. Medicinal plants are historically used in its treatment. The plant Cissampelos sympodialis, known in Northeastern Brazil as "Jarrinha" or "Milona", is used to treat some inflammatory conditions, including asthma. The objective of this study was to evaluate the potential of Cissampelos sympodialis EICHL. extract (CsE) and its isolated alkaloids, especially warifteine (Wa) on a Blomia tropicalis extract (BtE)-induced experimental model of allergy. The respiratory allergy was induced in AJ mice by the administration of BtE. Mice were orally treated with the 400 mg/kg of CsE or 8 mg/kg of total alkaloids fraction (TAF) or 4 mg/kg of Wa and the following parameters were analyzed: (a) total cell numbers in bronchoalveolar fluid (BAF); (b) differential cell numbers in BAF; (c) eosinophil peroxidase (EPO) activity in BAF; (d) IgE serum levels by passive cutaneous anaphylaxis; (e) IL-5, IL-13, IL-10, and IFN-γ levels in BAF; (f) histopathological alterations in the lung. The treatment of the animals with CsE, Wa or TAF led to a reduction in the numbers of total cells and eosinophils in BAF. The same reduction was observed in EPO levels in the BAF. The levels of IL-5 and IL-13 were also reduced in animals treated with Cissampelos sympodialis, while IL-10 levels were significantly increased in the BAF of CsE-treated animals. The treatment also decreased the density of inflammatory cells in the lung by histopathological examination demonstrating the potential of this medicinal plant as new agent for asthma treatment.
authors.affiliation: Instituto de Ciéncias da Saúde, Universidade Federal da Bahia, Bahia, Brazil.
authors.forename: Ana Tereza
authors.initials: AT
authors.lastname: Cerqueira-Lima
authors.forename: Neuza Maria
authors.initials: NM
authors.lastname: Alcântara-Neves
authors.forename: Lain Carlos Pontes
authors.initials: LC
authors.lastname: de Carvalho
authors.forename: Ryan Santos
authors.initials: RS
authors.lastname: Costa
authors.forename: José Maria
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.forename: Márcia
authors.initials: M
authors.lastname: Piuvezam
authors.forename: Russo
authors.initials: R
authors.lastname: Momtchilo
authors.forename: Renato
authors.initials: R
authors.lastname: Barboza
authors.forename: Eduardo
authors.initials: E
authors.lastname: de Jesus Oliveira
authors.forename: Alexsandro
authors.initials: A
authors.lastname: Marinho
authors.forename: Camila Alexandrina
authors.initials: CA
authors.lastname: Figueiredo
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D031606:Cissampelos / Q000737:chemistry*; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D047209:Eosinophil Peroxidase / Q000378:metabolism; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008805:Mice, Inbred A; D008925:Mites / Q000276:immunology*; D008962:Models, Theoretical; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D016207:Cytokines; D010936:Plant Extracts; D007073:Immunoglobulin E; C003931:warifteine; D047209:Eosinophil Peroxidase