title: [Analysis of full-length gene sequence of a rabies vaccine strain CTN-1 for human use in China].
abstract: CTN-1 is one of the rabies vaccine strains for human use in China, but there has been no report on the full-length gene sequence of CTN-1. In this study, the full-length gene of CTN-1 was amplified by RT-PCR, each PCR product was cloned into T vector and then sequenced, assemblied and compared with other vaccine strains as well as the wild Chinese rabies isolates. The phylogenetic tree of G gene was constructed and the genetic homology was analyzed. The results revealed that CTN-1 was 11 925nt (GenBank accession number: FJ959397)in length and belonged to the genotype I. The full-length nucleotide homologies among CTN-1 and other rabies virus strains were between 81.5%-93.4%, of which the lowest 81.5% was between CTN-1 strain and bat isolate SHBRV, and the highest 93.4% was between CTN-1 and Chinese isolate HN10. The phylogenetic analysis revealed that the majority of Chinese isolates could be grouped into the same clade with the CTN-1 strain, but aG and some vaccine strains from abroad such as Flury, PM, PV, ERA, RC-HL and a few Chinese strains were grouped in another clade. Comparsion of the G protein genes also showed that the homologies among CTN-1 and most of the Chinese isolates were higher than that of the other vaccine strains to those Chinese strains. Therefore, it suggests that the CTN-1 strain is more suitable and rational to be used for the production of rabies inactivated vaccine in China than the others.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Lei-tai
authors.initials: LT
authors.lastname: Shi
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Jing-hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Jian-rong
authors.initials: JR
authors.lastname: Tang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Wu
authors.forename: Shou-chun
authors.initials: SC
authors.lastname: Cao
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Qu
authors.forename: Guan-mu
authors.initials: GM
authors.lastname: Dong
mesh_terms: D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000517:prevention & control / Q000821:virology; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014765:Viral Vaccines / Q000235:genetics*
chemical_list: D014765:Viral Vaccines