title: Towards an optimized culture medium for the generation of mouse induced pluripotent stem cells.
abstract: Generation of induced pluripotent stem cells from somatic cells using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient and time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation of mouse induced pluripotent stem cells. This optimization of the culture medium is sensitive to the presence of Myc in the reprogramming factors. Moreover, we could reprogram meningeal cells using only two factors Oct4/Klf4. Therefore, iSF1 represents a basal medium that may be used for mechanistic studies and testing new reprogramming approaches.
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Jiekai
authors.initials: J
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Qingkai
authors.initials: Q
authors.lastname: Han
authors.forename: Dajiang
authors.initials: D
authors.lastname: Qin
authors.forename: Jianyong
authors.initials: J
authors.lastname: Xu
authors.forename: You
authors.initials: Y
authors.lastname: Chen
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Song
authors.forename: Dongshan
authors.initials: D
authors.lastname: Yang
authors.forename: Meixiu
authors.initials: M
authors.lastname: Peng
authors.forename: Wenzhi
authors.initials: W
authors.lastname: He
authors.forename: Ronghui
authors.initials: R
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gan
authors.forename: Ke
authors.initials: K
authors.lastname: Ding
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Esteban
authors.forename: Duanqing
authors.initials: D
authors.lastname: Pei
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000737:chemistry / Q000494:pharmacology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D016895:Culture Media, Serum-Free; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4