title: Microbial conversion and anticandidal effects of bioconverted product of cabbage (Brassica oleracea) by Pectobacterium carotovorum pv. carotovorum 21.
abstract: This study was undertaken to examine the anticandidal effects of microbially bioconverted product of cabbage, obtained from the microbial conversion of cabbage (Brassica oleracea var. capitata) by a bacterial strain Pectobacterium carotovorum pv. carotovorum 21 (Pcc 21) against various isolates of Candida species including a clinical isolate. The bioconverted product (10 microl, corresponding to 500 microg/disc) displayed potential anticandidal effect against Candida albicans KACC 30062, Candida geochares KACC 30061, Candida albicans KACC 30003, Candida saitoana KACC 41238 and Candida glabrata P00368 (clinical isolate) as a diameter of zones of inhibition, found in the range of 14 +/- 0.9 to 19 +/- 1.1mm. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of bioconverted product against the tested isolates were found in the range of 62.5-250 and 125-250 microg/ml, respectively. Also the bioconverted product had remarkable anticandidal effect on the viable counts of the tested Candida isolates. Further, scanning electron microscopic study revealed potential detrimental effect of bioconverted product on the morphology of C. albicans KACC 30062 at MIC concentration. All these findings together indicate that bioconverted product of cabbage has potential therapeutic value of medicinal significance to control Candida species including clinical isolates.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Vivek K
authors.initials: VK
authors.lastname: Bajpai
authors.forename: Sun Chul
authors.initials: SC
authors.lastname: Kang
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
authors.forename: Shruti
authors.initials: S
authors.lastname: Shukla
authors.forename: Seweon
authors.initials: S
authors.lastname: Lee
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D002175:Candida / Q000187:drug effects / Q000648:ultrastructure; D015169:Colony Count, Microbial; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D016973:Pectobacterium carotovorum / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents