title: Targeting Smad4 links microRNA-146a to the TGF-beta pathway during retinoid acid induction in acute promyelocytic leukemia cell line.
abstract: The expression pattern of microRNAs (miRNAs) and their potential target genes were investigated in acute promyelocytic leukemia (APL) cell line NB4 cells during all-trans-retinoid acid (ATRA) treatment by using a miRNA microarrays platform and real-time quantitative PCR (RTQ-PCR). MiR-146a as one of the miRNAs down-regulated by ATRA during APL differentiation was identified. Direct interaction between miR146a and its predictive target gene Smad4 were confirmed by Luciferase assay. Down-regulation of miR-146a and upregulation of Smad4 at protein levels were demonstrated. These data suggested that miR-146a might influence proliferation of APL cells through TGF-beta1/Smad signal transduction pathway during ATRA induction.
authors.affiliation: Department of Hematology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.forename: Ji-hua
authors.initials: JH
authors.lastname: Zhong
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Fang-yuan
authors.initials: FY
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D051901:Smad4 Protein / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D051901:Smad4 Protein; D016212:Transforming Growth Factor beta; D014212:Tretinoin