title: Systemic peripheral artery relaxation by KCNQ channel openers and hydrogen sulfide.
abstract: BACKGROUND
  Perivascular adipose tissue secretes an adipocyte-derived relaxing factor (ADRF) that opens voltage-dependent K (Kv) channels in peripheral arteries. We studied the role of KCNQ-type Kv channels and tested the hypothesis that hydrogen sulfide (H2S) could be an ADRF.
  
  
  METHODS
  We performed isometric contraction studies on systemic arteries of rats and mice.
  
  
  RESULTS
  In mesenteric arteries and aortas without perivascular adipose tissue, the KCNQ channel openers retigabine, VRX0530727, VRX0621238, and VRX0621688 produced concentration-dependent vasorelaxation; VRX0621688 was the most potent vasodilator. The KCNQ inhibitor XE991 (30 micromol/l) blocked the effects of both the drugs and ADRF. Inhibitors of cystathionine gamma lyase (CSE) beta-cyano-L-alanine (BCA, 5 mmol/l) and 4-propargyl glycine (PPG, 10 mmol/l) also blocked the relaxations. CSE is expressed in perivascular adipose tissue and endogenously generates H2S. The H2S donor NaHS produced concentration-dependent vasorelaxation, which was also blocked by XE991. The vasodilatory capacities of retigabine, VRX0530727, VRX0621238, and VRX0621688 were preserved following inhibition of H2S generation in perivascular fat.
  
  
  CONCLUSIONS
  We suggest that KCNQ channel opening is a powerful mechanism to produce vasorelaxation of systemic arteries in rats and mice. Furthermore, KCNQ channels play a major role in the paracrine control of vascular tone by perivascular adipose tissue, which is at least in part mediated or modulated by H2S. In conditions of reduced H2S release from perivascular adipose tissue, these paracrine effects can be mimicked by synthetic KCNQ channel openers.
authors.affiliation: Medical Clinic for Nephrology and Internal Intensive Care, Campus Virchow Klinikum, Experimental and Clinical Research Center and Max-Delbrück Center of Molecular Medicine, Berlin, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Schleifenbaum
authors.forename: Carolin
authors.initials: C
authors.lastname: Köhn
authors.forename: Nadezda
authors.initials: N
authors.lastname: Voblova
authors.forename: Galyna
authors.initials: G
authors.lastname: Dubrovska
authors.forename: Olga
authors.initials: O
authors.lastname: Zavarirskaya
authors.forename: Torsten
authors.initials: T
authors.lastname: Gloe
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Crean
authors.forename: Friedrich C
authors.initials: FC
authors.lastname: Luft
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Rudolf
authors.initials: R
authors.lastname: Schubert
authors.forename: Maik
authors.initials: M
authors.lastname: Gollasch
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D001158:Arteries / Q000187:drug effects* / Q000502:physiology*; D002219:Carbamates / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000378:metabolism*; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects / Q000502:physiology; D051656:KCNQ Potassium Channels / Q000819:agonists* / Q000037:antagonists & inhibitors / Q000502:physiology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D019899:Paracrine Communication / Q000187:drug effects / Q000502:physiology; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology*
chemical_list: C112297:10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone; D000873:Anthracenes; D002219:Carbamates; D051656:KCNQ Potassium Channels; D010655:Phenylenediamines; C101866:ezogabine; D006862:Hydrogen Sulfide