title: [Effect of combination of pulsed CO2 laser irradiation and diammine silver fluoride treatment on ultrastructure of dentine].
abstract: OBJECTIVE
  To explore the effect of combination of pulsed CO2 laser irradiation and diammine silver fluoride treatment on the ultrastructure of dentine.
  
  
  METHODS
  Each extracted molars was made into four identical dentinal specimens, which were randomly divided into 4 groups according to the treatment methods: A, control group; B, pulsed CO2 laser irradiation; C, coated with 38%Ag(NH3)2F; and D, laser irradiation plus fluoride. Scanning electron microscope (SEM) analysis was performed.
  
  
  RESULTS
  As shown by SEM, group A showed clear dentinal tubuli, group B showed melted surface without dentinal tubule openings, group C showed thicker melted layer without dentinal tubule openings, and group D showed the thickest melted and recrystallized layer on dentine surface.
  
  
  CONCLUSIONS
  Both pulsed CO2 laser irradiation and 38%Ag(NH3)2F treatment can seal dentinal tubule. The combination of these two techniques can form a synergistic effect.
authors.affiliation: Department of Stomatology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Ji-Zhi
authors.initials: JZ
authors.lastname: Zhao
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wu
mesh_terms: D003804:Dentin / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D007834:Lasers; D054020:Lasers, Gas; D018030:Silver Compounds / Q000494:pharmacology*
chemical_list: D018030:Silver Compounds; C105022:silver fluoride; D005459:Fluorides