Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
Tian
Yong-Sheng
YS
Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China.
Xiong
Ai-Sheng
AS
Xu
Jing
J
Zhao
Wei
W
Gao
Feng
F
Fu
Xiao-Yan
XY
Xu
Hu
H
Zheng
Jian-Li
JL
Peng
Ri-He
RH
Yao
Quan-Hong
QH
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015870:Gene Expression; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D020621:Ochrobactrum anthropi / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000097797:Glyphosate
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D005998:Glycine; D000097797:Glyphosate