The antiinflammatory potential of phenolic compounds from Emblica officinalis L. in rat.
Antiinflammatory effects of phenolic compounds from Emblica officinalis were evaluated in carrageenan and cotton pellet induced acute and chronic inflammatory animal model. Fractions of E. officinalis containing free (FPEO) and bounded (BPEO) phenolic compounds were assessed by HPLC technique. The free and bound phenolic compounds were studied for their acute and chronic antiinflammatory activity at dose level of 20 and 40 mg/kg. The carrageenan induced acute inflammation was assessed by measuring rat paw volume at different time of intervals. Further, cotton pellet induced chronic inflammation was assessed by granulomatous tissue mass estimation along with the estimation of tissue biomarker changes (i.e. lipid peroxidation, reduced glutathione, myeloperoxidase and plasma extravasation). The results indicated that in both acute and chronic inflammation, FPEO and BPEO show reduction in the inflammation, but significant effects was observed only at high doses of both fractions which was comparable to diclofenac treated group. In conclusion, phenolic compounds of E. officinalis may serve as potential herbal candidate for amelioration of acute and chronic inflammation due to their modulatory action of free radicals.
Muthuraman
Arunachalam
A
Rayat Institute of Pharmacy, Nawanshahr District, Near Railmajra, Ropar, 144533 Punjab, India. arunachalammu@gmail.com
Sood
Shailja
S
Singla
Sumeet Kumar
SK
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002351:Carrageenan / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000494:pharmacology; D004487:Edema / Q000188:drug therapy; D029001:Herbal Medicine / Q000379:methods; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D029842:Phyllanthus emblica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D010936:Plant Extracts; D004008:Diclofenac; D002351:Carrageenan