Altered salivary redox homeostasis in patients with systemic sclerosis.
OBJECTIVE
Oxidative stress has been implicated in the pathogenesis of systemic sclerosis (SSc). Our objective was to determine whether SSc is associated with altered redox homeostasis in human saliva.


METHODS
Study participants were 70 women with SSc and 120 female controls. 8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-epi-prostaglandin F 2alpha (8-epi-PGF2alpha), and total protein carbonyls were assayed by ELISA to quantify oxidative damage to nucleic acids, lipids, and proteins, respectively, in whole nonstimulated saliva.


RESULTS
We observed a significantly positive association between salivary log protein carbonyls and SSc in a crude statistic (OR 9.06, p < 0.0001), and multivariable model adjusted for log 8-OHdG, log 8-epi-PGF2alpha, and antioxidant exposure (OR 9.26, p < 0.0001). No significant association was noted between SSc and salivary log 8-epi-PGF2alpha or log 8-OHdG.


CONCLUSIONS
Salivary redox homeostasis is perturbed in patients with SSc and may inform on the pathophysiology and presence of the disease (biomarkers) and efficacy of therapeutic interventions.
Su
Haixiang
H
Centre for Neurotranslational Research, Lady Davis Institute for Medical Research, McGill University.
Baron
Murray
M
Benarroch
Michael
M
Velly
Ana M
AM
Gravel
Sabrina
S
Schipper
Hyman M
HM
Gornitsky
Mervyn
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006706:Homeostasis; D006801:Humans; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D012463:Saliva / Q000737:chemistry*; D012595:Scleroderma, Systemic / Q000378:metabolism* / Q000503:physiopathology
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine