A method to site-specifically introduce methyllysine into proteins in E. coli.
A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.
Ai
Hui-Wang
HW
Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
Lee
Jae Wook
JW
Schultz
Peter G
PG
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D008239:Lysine / Q000031:analogs & derivatives* / Q000235:genetics; D009154:Mutation; D012343:RNA, Transfer / Q000235:genetics*
D029968:Escherichia coli Proteins; C049907:2-methyllysine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine