title: [Effects of astilbin on the expression of TNF alpha and IL-10 in liver warm ischemia-reperfusion injury].
abstract: OBJECTIVE
  To investigate the effects of astilbin on the expressions of TNF alpha and IL-10 during liver warm ischemia-reperfusion injury.
  
  
  METHODS
  C57BL/ 6 mice were randomly divided into 4 groups (n = 8): sham-operated group (Sham), model control group(I/R), low dosage of astilbin treatment group (10 mg/kg) and high dosage of astilbin (40 mg/kg) treatment group. The treatment group mice were intraperitoneally injected with 10 or 40 mg/kg astilbin 24 hours and one hour before Ischemia, the hepatic ischemia-reperfusion model were thus established. After jn90 of min ischemia and 6 h reperfusion of the partial hepatic lobe, the expressions of TNF alpha and IL-10 in liver tissues collected from the experimental groups were detected by Western blot and semiquantitative RT-PCR.
  
  
  RESULTS
  The expression of TNF alpha protein in liver tissues gradually decreased in treatment groups (low and high dosages of astilbin treatment groups) as compared to the I/R model control group. Similar results were observed in the mRNA expressions of these genes as determined by semiquantitative RT-PCR (P less than 0.05 for low dosage group; P less than 0.01 for high dosage group). Compared with the I/R model control group, the expression of IL-10 was increased in both treatment groups (low dosage group P less than 0.05; large dosage group P less than 0.01).
  
  
  CONCLUSIONS
  Treatment with astilbin decreases TNF alpha expression but induces IL-10 expression in liver during warm ischemia-reperfusion injury.
authors.affiliation: Center of Liver Transplantation, the 180 Hospital of PLA, Quanzhou Fujian 362000, China. lrk7618401@163.com
authors.forename: Rong-Kai
authors.initials: RK
authors.lastname: Lin
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Mu
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Yao
authors.forename: Shao-Liang
authors.initials: SL
authors.lastname: Dong
authors.forename: Qiu-Bao
authors.initials: QB
authors.lastname: Ai
authors.forename: Quan-Xing
authors.initials: QX
authors.lastname: Wang
mesh_terms: D000818:Animals; D044948:Flavonols / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D052096:Warm Ischemia
chemical_list: D044948:Flavonols; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C099069:astilbin