title: Sublingual immunization with an HIV subunit vaccine induces antibodies and cytotoxic T cells in the mouse female genital tract.
abstract: A vaccine against heterosexual transmission by human immunodeficiency virus (HIV) should generate cytotoxic and antibody responses in the female genital tract and in extra-genital organs. We report that sublingual immunization with HIV-1 gp41 and a reverse transcriptase polypeptide coupled to the cholera toxin B subunit (CTB) induced gp41-specific IgA antibodies and antibody-secreting cells, as well as reverse transcriptase-specific CD8 T cells in the genital mucosa, contrary to intradermal immunization. Conjugation of the reverse transcriptase peptide to CTB favored its cross-presentation by human dendritic cells to a T cell line from an HIV(+) patient. Sublingual vaccination could represent a promising vaccine strategy against heterosexual transmission of HIV-1.
authors.affiliation: Inserm, U634, Faculté de Médecine Pasteur, 06107 Nice cedex 2, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Hervouet
authors.forename: Carmelo
authors.initials: C
authors.lastname: Luci
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cuburu
authors.forename: Magali
authors.initials: M
authors.lastname: Cremel
authors.forename: Selma
authors.initials: S
authors.lastname: Bekri
authors.forename: Lene
authors.initials: L
authors.lastname: Vimeux
authors.forename: Concepcion
authors.initials: C
authors.lastname: Marañon
authors.forename: Cecil
authors.initials: C
authors.lastname: Czerkinsky
authors.forename: Anne
authors.initials: A
authors.lastname: Hosmalin
authors.forename: Fabienne
authors.initials: F
authors.lastname: Anjuère
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000286:Administration, Sublingual; D000818:Animals; D045142:Cross-Priming; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D005836:Genitalia, Female / Q000276:immunology* / Q000821:virology; D015483:HIV Antibodies / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D015700:HIV Envelope Protein gp41; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C514787:gp41 protein, Human immunodeficiency virus 1