Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei.
Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both RNAi-related and innate antiviral responses. They also suggest that these two mechanisms of antiviral response may activate the same pathway, requiring Lv-Sid 1 and Lv-Ago 2 induction.
Labreuche
Yannick
Y
Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, 221 Ft. Johnson Road, Charleston, SC 29412, USA. yannick.labreuche@ifremer.fr
Veloso
Artur
A
de la Vega
Enrique
E
Gross
Paul S
PS
Chapman
Robert W
RW
Browdy
Craig L
CL
Warr
Gregory W
GW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology*; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D033561:Penaeidae; D010802:Phylogeny; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000276:immunology* / Q000472:pathogenicity
D039642:Eukaryotic Initiation Factors; D012330:RNA, Double-Stranded; D011494:Protein Kinases; C409036:Sid1 kinase