title: Prolonged interval between fusion and activation impairs embryonic development by inducing chromosome scattering and nuclear aneuploidy in pig somatic cell nuclear transfer.
abstract: The aim of the present study was to examine the effect of various intervals between electrofusion and activation (FA interval) on the nuclear remodelling and development of somatic cell nuclear transfer (SCNT) embryos in pigs. Reconstructed oocytes were activated at 0 (simultaneous fusion and activation; SFA), 1, 2 and 3 h (delayed activation) after electrofusion; these groups were designated as DA1, DA2 and DA3, respectively. When oocyte nuclear status was examined at 0.5, 1, 2 and 3 h after electrofusion, the incidence of chromosome scattering was increased (P < 0.01) as the FA interval was extended (0.0%, 12.0%, 77.3% and 78.0%, respectively). Extending the FA interval led to an increase (P < 0.01) in the percentage of oocytes containing multiple (>or=3) pseudopronuclei (PPN) (0.0% of SFA; 5.3% of DA1; 21.7% of DA2; and 33.5% of DA3). The development of SCNT embryos to the blastocyst stage was decreased (P < 0.05) in DA2 (5.7%) and DA3 (5.0%) compared with SFA (18.1%) and DA1 (19.5%). Our results demonstrate that extending the FA interval impairs the development of SCNT pig embryos by inducing chromosome scattering and the formation of multiple PPN, which may result in increased nuclear aneuploidy.
authors.affiliation: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: You
authors.forename: Kilyoung
authors.initials: K
authors.lastname: Song
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
mesh_terms: D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology*; D013552:Swine; D013997:Time Factors