title: Biotinylated lipid bilayer disks as model membranes for biosensor analyses.
abstract: The aim of this study was to investigate the potential of polyethylene glycol (PEG)-stabilized lipid bilayer disks as model membranes for surface plasmon resonance (SPR)-based biosensor analyses. Nanosized bilayer disks that included 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)(2000)] (DSPE-PEG(2000)-biotin) were prepared and structurally characterized by cryo-transmission electron microscopy (cryo-TEM) imaging. The biotinylated disks were immobilized via streptavidin to three different types of sensor chips (CM3, CM4, and CM5) varying in their degree of carboxymethylation and thickness of the dextran matrix. The bilayer disks were found to interact with and bind stably to the streptavidin-coated sensor surfaces. As a first step toward the use of these bilayer disks as model membranes in SPR-based studies of membrane proteins, initial investigations were carried out with cyclooxygenases 1 and 2 (COX 1 and COX 2). Bilayer disks were preincubated with the respective protein and thereafter allowed to interact with the sensor surface. The signal resulting from the interaction was, in both cases, significantly enhanced as compared with the signal obtained when disks alone were injected over the surface. The results of the study suggest that bilayer disks constitute a new and promising type of model membranes for SPR-based biosensor studies.
authors.affiliation: Department of Physical and Analytical Chemistry, BMC, Uppsala University, SE-751 23 Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Lundquist
authors.forename: Søren B
authors.initials: SB
authors.lastname: Hansen
authors.forename: Helena
authors.initials: H
authors.lastname: Nordström
authors.forename: U Helena
authors.initials: UH
authors.lastname: Danielson
authors.forename: Katarina
authors.initials: K
authors.lastname: Edwards
mesh_terms: D015374:Biosensing Techniques; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry*; D019921:Biotinylation; D020285:Cryoelectron Microscopy; D051545:Cyclooxygenase 1 / Q000737:chemistry / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D008051:Lipid Bilayers; D008567:Membranes, Artificial; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D001710:Biotin; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2