Assessment of interactions between mucins of gastric juice and Helicobacter pylori--preliminary study.
OBJECTIVE
Helicobacter pylori resides primarily in the gastric mucus layer composed of carbohydrate-rich glycoproteins, mucins. Carbohydrates of the secretory MUC 5AC mucin are one of the proved receptors for H. pylori adhesins. A participation of the membrane-associated MUC 1 in the mechanism of infection is also suggested. The main aim of the study was to support the participation of the membrane associated MUC 1 mucin in the mechanism of infection.


METHODS
13 gastric juices were included in the study. The presence of MUC 5AC and MUC 1 mucins as well as H. pylori bindings were performed using ELISA tests.


RESULTS
MUC 1 and MUC 5AC mucins were present in all the examined juices. H. pylori adhered to both glycoproteins.


CONCLUSIONS
H. pylori bind to the secretory MUC 5AC mucin as well as to the epithelial MUC 1. This supports the idea that the membrane-associated mucin is involved in the mechanism of H. pylori infection.
Radziejewska
Iwona
I
Medical University of Białystok, Department of Medical Chemistry, ul. Mickiewicza 2a, 15-879 Białystok 8, Poland. iwona@umwb.edu.pl
Leszczyńska
Katarzyna
K
Borzym-Kluczyk
Małgorzata
M
Namiot
Zbigniew
Z
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000378:metabolism*; D018396:Mucin-1 / Q000378:metabolism*
D055271:Mucin 5AC; D018396:Mucin-1