title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the peptidoglycan binding region of the Ser/Thr kinase PrkC from Staphylococcus aureus.
abstract: PrkC is an important Ser/Thr membrane kinase of Staphylococcus aureus able to bind peptidoglycans through extra-cellular domains, denominated as PASTA. Upon peptidoglycan binding, PrkC is activated and stimulates bacterial growth and revival from latency. The entire extra-cellular region of PrkC (residues 378-664), containing three predicted PASTA domains and an extra-domain of unknown function, has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and refinement is in progress.
authors.affiliation: Institute of Biostructures and Bioimaging, CNR, Napoli, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ruggiero
authors.forename: Flavia
authors.initials: F
authors.lastname: Squeglia
authors.forename: Viviana
authors.initials: V
authors.lastname: Izzo
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Luigi
authors.initials: L
authors.lastname: Vitagliano
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Rita
authors.initials: R
authors.lastname: Berisio
mesh_terms: D018360:Crystallography, X-Ray; D010457:Peptidoglycan / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D010457:Peptidoglycan; D017346:Protein Serine-Threonine Kinases