hnRNP Q and PTB modulate the circadian oscillation of mouse Rev-erb alpha via IRES-mediated translation.
The physiological and behavioral circadian rhythms of most creatures are controlled by a harmony of functional relationships between clock genes. In mammals, several core clock genes show rhythmic profiles of their mRNA and protein expression. Among them, Rev-erb α functions as a transcriptional repressor, affecting expression patterns of other clock genes. For the continuous and robust oscillation of the molecular clock system, the levels of Rev-erb α protein are expected to be tightly regulated with the correct timing. Here, we demonstrate that Rev-erb α has an internal ribosomal entry site (IRES) in its 5' untranslated region. Furthermore, we demonstrate that heterogeneous nuclear ribonucleoprotein Q and polypyrimidine tract-binding protein (PTB) modulate the IRES-mediated translation of Rev-erb α. We suggest that the rhythmic binding affinity of hnRNP Q to the Rev-erb α IRES and the change in PTB cytosolic levels lead to maintenance of the oscillation profile of the Rev-erb α protein.
Kim
Do-Yeon
DY
Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Gyeong-Buk, 790-784, Republic of Korea.
Woo
Kyung-Chul
KC
Lee
Kyung-Ha
KH
Kim
Tae-Don
TD
Kim
Kyong-Tai
KT
D020121:5' Untranslated Regions; D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics*; D005786:Gene Expression Regulation; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D041681:NIH 3T3 Cells; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000096:biosynthesis / Q000235:genetics*; D038941:Polypyrimidine Tract-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014176:Protein Biosynthesis; D034622:RNA Interference; D020123:Sirolimus / Q000494:pharmacology
D020121:5' Untranslated Regions; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C498262:Nr1d1 protein, mouse; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D038941:Polypyrimidine Tract-Binding Protein; D020123:Sirolimus