Plasma thiols and taurine levels in central retinal vein occlusion.
OBJECTIVE
To determine the plasma levels of the sulfur-containing amino-acids homocysteine, cysteine, cysteinylglycine, glutamylcysteine, glutathione, and taurine in patients with central retinal vein occlusion (CRVO) and in healthy subjects and to ascertain whether there are statistically significant differences between patients and controls.


METHODS
Laser-induced fluorescence capillary electrophoresis was used to measure the plasma levels of homocysteine, cysteine, cysteinylglycine, glutamylcysteine, glutathione, and taurine in 29 patients with CRVO and 80 age- and gender-matched control subjects. Wilcoxon or Student's t-test was used, when appropriate, to determine differences between groups. Multivariate logistic regression analysis was used to determine the risks for CRVO.


RESULTS
CRVO patients showed significantly higher concentrations of cysteine (p = 0.032) and significantly lower concentrations of cysteinylglycine (p = 0.009) and taurine (p = 0.0002) than controls. Conversely, there were no significant differences in plasma homocysteine, glutamylcysteine, and glutathione between CRVO patients and controls. When categorized by CRVO type (ischemic/non-ischemic), taurine was still lower in both subgroups than in controls, whereas cysteine, cysteinylglycine, as well as homocysteine, were significantly higher only in the ischemic subgroup. In non-ischemic CRVO, cysteinylglycine fell just short of statistical significance (p = 0.06). Logistic regression analysis revealed an odds ratio of 1.02 (95% confidence interval (CI): 1.01-1.04, p = 0.001) for cysteine, 0.79 (95% CI: 0.70-0.89, p = 0.0002) for cysteinylglycine, and 0.94 (95% CI: 0.90-0.97, p = 0.002) for taurine.


CONCLUSIONS
Results suggest that reduced plasma levels of cysteinylglycine and taurine may contribute to the pathogenesis of both CRVO types. Furthermore, this study also demonstrated an association between ischemic CRVO and higher concentrations of homocysteine and cysteine.
Pinna
Antonio
A
Institute of Ophthalmology, University of Sassari, Sassari, Italy. apinna@uniss.it
Zinellu
Angelo
A
Franconi
Flavia
F
Carru
Ciriaco
C
D000368:Aged; D016022:Case-Control Studies; D003404:Creatinine / Q000097:blood; D003545:Cysteine / Q000097:blood; D004151:Dipeptides / Q000097:blood; D019075:Electrophoresis, Capillary; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D012170:Retinal Vein Occlusion / Q000097:blood* / Q000145:classification; D013438:Sulfhydryl Compounds / Q000097:blood*; D013654:Taurine / Q000097:blood*
D004151:Dipeptides; D008055:Lipids; D013438:Sulfhydryl Compounds; D013654:Taurine; C028505:cysteinylglycine; D003404:Creatinine; D005978:Glutathione; D003545:Cysteine; C017341:gamma-glutamylcysteine