title: Pattern of matrix metalloproteinases-9, P53 and BCL-2 proteins in Egyptian patients with pulmonary Mycobacterium tuberculosis.
abstract: Matrix metalloproteinases (MMPs) constitute a large family of enzymes that degrade extracellular matrix proteins (ECM). MMPs are implicated in different pathological conditions such as cancer. Bcl-2 and P53 are key controllers of programmed cell death (PCD) or apoptosis. The aim of the present study was to determine the MMP-9, P53 and Bcl-2 levels in Egyptian patients with Mycobacterium tuberculosis (MTB) (Group I) compared with healthy control individuals (Group II). The concentrations of serum MMP-9 were determined quantitatively using enzyme immunoassay (EIA). P53 and Bcl-2 levels were assayed by flow cytometric analysis using specific monoclones. MMP-9 level was significantly higher in MTB patients compared with healthy control. Similarly, P53 and Bcl-2 levels were increased in MTB patients compared with healthy ones. These data reflect the alteration of MMP-9 level during the course of MTB infection, accompanied with apparent dysregulation of cellular apoptosis as indicated by P53 and Bcl-2 over-expression.
authors.affiliation: Minufiya University Molecular and Cellular Biology Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Sadat City, Minufiya, Egypt.
authors.forename: Samir
authors.initials: S
authors.lastname: El-Masry
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Lotfy
authors.forename: Mona
authors.initials: M
authors.lastname: Samy
authors.forename: Shadin
authors.initials: S
authors.lastname: Moawia
authors.forename: Ibrahim H
authors.initials: IH
authors.lastname: El-Sayed
authors.forename: Islam M
authors.initials: IM
authors.lastname: Khamees
mesh_terms: D000328:Adult; D017209:Apoptosis; D005260:Female; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000097:blood*; D014397:Tuberculosis, Pulmonary / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000097:blood*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D020780:Matrix Metalloproteinase 9