title: Quality differences between NILs of wheat variety Long 97-586 possessing HMW-GS 7+8 and 7.
abstract: The high molecular weight glutenin subunits (HMW-GS) 7+8 were introduced into the Long 97-586 (1, 7, 2+12) wheat variety (Triticum aestivum) by 5 consecutive backcrosses with biochemical marker-assisted selection. Nearly isogenic lines (NILs) of HMW-GS 7 and 7+8 were obtained, and the NILs were planted in the experimental field at the Crop Breeding Institute of Heilongjiang Academy of Agricultural Science in 2004-2006. The field experiments were designed using the two-column contrast arrangement method with six replicates in 2004-2005 and four replicates in 2006. The result of three years experiments showed that the differences between NILs of Long 97-586 with subunit 7 and those with subunits 7+8 in the quality parameters of flour protein content and dry gluten content were negligible (P>0.1). However, the differences in some of the quality parameters were remarkably significant (P<0.01), including wet gluten content, ratio of wet gluten/dry gluten, gluten index, Zeleny sedimentation, ratio of sedimentation/dry gluten, and the farinogram parameters of water absorption, development time, stability, breakdown time and degree of softening. The difference between NILs with subunits 7+8 and subunit 7 was significant (P<0.05) on the alveogram W value and had a critical value (P=0.05) on the alveogram P value in 2006. The results show that HMW-GS 7+8 is far superior to HMW-GS 7 in terms of baking quality. The possibilities of using subunits 7+8 and subunit 7 in breeding strong and weak gluten wheat varieties are discussed in this paper.
authors.affiliation: Crop Breeding Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.
authors.forename: LiLi
authors.initials: L
authors.lastname: Zhang
authors.forename: YanBin
authors.initials: Y
authors.lastname: Zhang
authors.forename: JiLin
authors.initials: J
authors.lastname: Li
authors.forename: HaiBin
authors.initials: H
authors.lastname: Zhao
authors.forename: QingJie
authors.initials: Q
authors.lastname: Song
authors.forename: HaiYang
authors.initials: H
authors.lastname: Yu
authors.forename: ChunLi
authors.initials: C
authors.lastname: Zhang
authors.forename: WenLi
authors.initials: W
authors.lastname: Xin
authors.forename: ZhiMin
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D001947:Breeding; D005433:Flour; D005983:Glutens / Q000737:chemistry / Q000235:genetics*; D008970:Molecular Weight; D006109:Poaceae / Q000235:genetics; D014908:Triticum / Q000737:chemistry / Q000235:genetics
chemical_list: D005983:Glutens; C037302:glutenin