Mitochondrial function in heart and kidney of spontaneously hypertensive rats: influence of captopril treatment.
Effect of captopril treatment on capability of heart and kidney mitochondria to produce ATP was investigated in spontaneously hypertensive rats (SHR). Heart mitochondria from SHR responded to hypertension with tendency to compensate the elevated energy demands of cardiac cells by moderate increase in mitochondrial Mg2+-ATPase activity, membrane fluidity (MF) and in majority of functional parameters of the mitochondria (p>0.05). Significant increase exhibited only the oxygen consumption (QO2; p<0.01-0.001) and oxidative phosphorylation rate (OPR; p<0.003) with glutamate+malate (GLUT+MAL) as substrates. Lowering the blood pressure (p<0.02) captopril also eliminated the above compensatory response and impaired the oxidative ATP production by decreasing OPR (p<0.001). Kidney mitochondria of SHR experienced serious disarrangement in parameters of oxidative ATP production: increase in Mg2+-ATPase activity (p<0.05) but, also scattered QO2 values (p<0.03-0.01) leading to decrease in OPR and the ADP:O (p<0.05-0.01) values with both GLUT+MAL and succinate as substrates. Captopril treatment does not alleviated but even worsened the above alterations. Mg2+-ATPase became also decreased and the depression of ADP:O became aggravated (p<0.0001).
Mujkosová
Jana
J
Institute for Heart Research, Centre of Excellence for Cardiovascular Research, Slovak Academy of Sciences, 840 05 Bratislava, Slovakia. jana.mujkosova@gmail.com
Ulicná
Olga
O
Waczulíková
Iveta
I
Vlkovicová
Jana
J
Vancová
Ol'ga
O
Ferko
Miroslav
M
Polák
Stefan
S
Ziegelhöffer
Attila
A
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D000255:Adenosine Triphosphate; D002216:Captopril; D017301:Ca(2+) Mg(2+)-ATPase