title: Dendritic cells loaded with tumor B cells elicit broad immunity against murine gammaherpesvirus 68 but fail to prevent long-term latency.
abstract: It is still unknown whether a noninfectious gammaherpesvirus vaccine is able to prevent or reduce virus persistence. This led us to use dendritic cells loaded with tumor B cells as a vaccine approach for the murine gammaherpesvirus 68 (gammaHV68) model of infection. Dendritic cells loaded with UV-irradiated latently infected tumor B cells induce broad, strong, and long-lasting immunity against gammaHV68. Dendritic cell vaccination prevents the enlargement of lymph nodes and severely limits acute infection and early latency but does not prevent gammaHV68 from establishing long-term latency. Our findings support the concept that attenuated viruses may be the best vaccine option for preventing gammaherpesvirus persistence.
authors.affiliation: Center for Vaccines and Immunity, Research Institute at Nationwide Children's Hospital, Columbus, Ohio 43205, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Weslow-Schmidt
authors.forename: Fang
authors.initials: F
authors.lastname: Ye
authors.forename: Stephanie S
authors.initials: SS
authors.lastname: Cush
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Stuller
authors.forename: Marcia A
authors.initials: MA
authors.lastname: Blackman
authors.forename: Emilio
authors.initials: E
authors.lastname: Flaño
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D018142:Gammaherpesvirinae / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006566:Herpesviridae Infections / Q000276:immunology* / Q000821:virology; D006801:Humans; D016393:Lymphoma, B-Cell / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D017735:Virus Latency
chemical_list: D019496:Cancer Vaccines