title: Fecal coliform accumulation within a river subject to seasonally-disinfected wastewater discharges.
abstract: As pathogen contamination is a leading cause of surface water impairment, there has been increasing interest in the implications of seasonal disinfection practices of wastewater effluents for meeting water quality goals. For receiving waters designated for recreational use, disinfection during the winter months is often considered unnecessary due to reduced recreational usage, and assumptions that lower temperatures may reduce pathogen accumulation. For a river subject to seasonal disinfection, we sought to evaluate whether fecal coliforms accumulate during the winter to concentrations that would impair river water quality. Samples were collected from municipal wastewater outfalls along the river, as well as upstream and downstream of each outfall during the winter, when disinfection is not practiced, and during the summer, when disinfection is practiced. During both seasons, fecal coliform concentrations reached 2000-5000 CFU/100 mL, nearly an order of magnitude higher than levels targeted for the river to achieve primary contact recreational uses. During the summer, wastewater effluents were not significant contributors to fecal coliform loadings to the river. During the winter, fecal coliform accumulated along the river predominantly due to loadings from successive wastewater outfalls. In addition to the exceedance of fecal coliform criteria within the river, the accumulation of wastewater-derived fecal coliform along the river during the winter season suggests that wastewater outfalls may contribute elevated loads of pathogens to the commercial shellfish operations occurring at the mouth of the river. Reductions in fecal coliform concentrations between wastewater outfalls were attributed to dilution or overall removal. Combining discharge measurements from gauging stations, tributaries and wastewater outfalls to estimate seepage, dilution between wastewater outfalls was estimated, along with the percentage of the river deriving from wastewater outfalls. After accounting for dilution, the residual reductions in fecal coliform concentrations observed between outfalls were attributed to actual fecal coliform removal. The estimated rate of removal of 1.52 d(-1) was significantly higher than die-off rates determined by previous researchers at similarly low temperatures in laboratory batch experiments, indicating the potential importance of other removal mechanisms, such as predation or sedimentation.
authors.affiliation: Department of Chemical Engineering, Yale University, Mason Lab 313b, 9 Hillhouse Avenue, New Haven, CT 06520, USA.
authors.forename: Azalea A
authors.initials: AA
authors.lastname: Mitch
authors.forename: Katherine C
authors.initials: KC
authors.lastname: Gasner
authors.forename: William A
authors.initials: WA
authors.lastname: Mitch
mesh_terms: D004203:Disinfection / Q000379:methods*; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D004784:Environmental Monitoring; D005243:Feces / Q000382:microbiology*; D011998:Recreation; D018570:Risk Assessment; D045483:Rivers / Q000382:microbiology*; D012621:Seasons; D014865:Waste Disposal, Fluid / Q000379:methods*; D014876:Water Pollution / Q000032:analysis*