Pressurized capillary electrochromatography with indirect amperometric detection for analysis of organophosphorus pesticide residues.
A new analytical method, pressurized capillary electrochromatography with indirect amperometric detection, has been developed for the determination of some non-electroactive organophosphorus pesticides (OPPs). When 0.1 mmol L(-1) of 3,4-dihydroxybenzylamine (DHBA) was added to the mobile phase containing 50% v/v of ACN and 50% v/v of MES buffer (10 mmol L(-1), pH 5.5), and +0.9 V (vs. Ag/AgCl) of working potential were used, maximal signal levels of analytes could be achieved. A separation voltage of +10 kV, a column pressure of 7.0 MPa and a pump flow rate of 0.05 mL min(-1) were selected as the other optimal conditions for separation of six OPPs, namely, dimethoate, methyl parathion, ethyl parathion, chlorpyrifos, chlorpyrifos-methyl, trichlorfon. The OPPs could be separated within 15 min and determined with the detection limits ranging from 0.008 to 0.2 mg/kg. Combining with a solid phase extraction procedure, mean recoveries between 78.9 and 87.2% for vegetable samples and from 81.4 to 98.6% for fruit samples were obtained.
Wu
Weimin
W
College of Chemistry and Chemical Engineering, Fuzhou University, 350108, China.
Wu
Yimin
Y
Zheng
Minmin
M
Yang
Liuming
L
Wu
Xiaoping
X
Lin
Xucong
X
Xie
Zenghong
Z
D053801:Capillary Electrochromatography / Q000379:methods*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D011312:Pressure
D009943:Organophosphorus Compounds; D010573:Pesticide Residues