Post-transcriptional gene silencing of root-knot nematode in transformed soybean roots.
RNAi constructs targeted to four different genes were examined to determine their efficacy to reduce galls formed by Meloidogyne incognita in soybean roots. These genes have high similarity with essential soybean cyst nematode (Heterodera glycines) and Caenorhabditis elegans genes. Transformed roots were challenged with M. incognita. Two constructs, targeted to genes encoding tyrosine phosphatase (TP) and mitochondrial stress-70 protein precursor (MSP), respectively, strongly interfered with M. incognita gall formation. The number of galls formed on roots transformed with constructs targeting the M. incognita TP and MSP genes was reduced by 92% and 94.7%, respectively. The diameter of M. incognita inside these transformed roots was 5.4 and 6.5 times less than the diameter of M. incognita found inside control plants transformed with the empty vector. These results indicate that silencing the genes encoding TP and MSP can greatly decrease gall formation and shows a promising solution for broadening resistance of plants against this plant-parasitic nematode.
Ibrahim
Heba M M
HM
United States Department of Agriculture, Plant Sciences Institute, Beltsville, MD 20705, USA.
Alkharouf
Nadim W
NW
Meyer
Susan L F
SL
Aly
Mohammed A M
MA
Gamal El-Din
Abd El Kader Y
Ael K
Hussein
Ebtissam H A
EH
Matthews
Benjamin F
BF
D000818:Animals; D015801:Helminth Proteins / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D018517:Plant Roots / Q000469:parasitology; D010941:Plant Tumors / Q000469:parasitology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D034622:RNA Interference; D018510:RNA, Helminth / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000469:parasitology*; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development
D015801:Helminth Proteins; D018510:RNA, Helminth; D007770:L-Lactate Dehydrogenase; D017027:Protein Tyrosine Phosphatases