T regulatory cells control antigen-induced recruitment of mast cell progenitors to the lungs of C57BL/6 mice.
In C57BL/6 mice, the recruitment of mast cell progenitors (MCps) to the lung is a feature of Ag-induced pulmonary inflammation that requires sensitization and challenge and is totally inhibited by the administration of anti-CD4 at the time of challenge. When mAb to TGFbeta1 or to IL-10R was administered at the time of challenge, the recruitment of MCp/10(6) mononuclear cells (MNCs) to the lung was inhibited by 56.3 and 69.6%, respectively, whereas mAb to IL-4, IFN-gamma, IL-6, IL-17A, and IL-17F had no effect. In sensitized and challenged C57BL/6 mice lacking TGFbetaRII on CD4(+) cells, the recruitment of MCp/10(6) MNCs was reduced by 67.8%. The requirement for TGFbeta1 and IL-10 suggested a role for CD4(+)CD25(+) T regulatory cells. Mice treated with anti-CD25 at the time of Ag-challenge showed a reduction in the recruitment of MCp/10(6) MNCs by 77.2% without any reduction in MNC influx. These results reveal an unexpected role for T regulatory cells in promoting the recruitment of MCps to the lungs of C57BL/6 mice with Ag-induced pulmonary inflammation.
Jones
Tatiana G
TG
Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Smith Research Building, Room 616, One Jimmy Fund Way, Boston, MA 02115, USA.
Finkelman
Fred D
FD
Austen
K Frank
KF
Gurish
Michael F
MF
D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D015704:CD4 Antigens / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D003888:Desensitization, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage* / Q000276:immunology; D007274:Injections, Intraperitoneal; D008168:Lung / Q000166:cytology / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013234:Stem Cells / Q000166:cytology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D018984:Epitopes, T-Lymphocyte; D010047:Ovalbumin