Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4.
This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30°C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (∼1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
Iwata
Kenichi
K
College of Systems Engineering and Science, Department of Bioscience and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama-shi, Saitama 337-8570 Japan.
Azlan
Azlin
A
Yamakawa
Hironori
H
Omori
Toshio
T
D000641:Ammonia / Q000378:metabolism*; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053528:Lysobacter / Q000235:genetics / Q000378:metabolism*; D009586:Nitrogen Fixation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
D003470:Culture Media; D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D000641:Ammonia; D005947:Glucose