title: Performance of the Aptima HIV-1 RNA qualitative assay with 16- and 32-member specimen pools.
abstract: The Aptima HIV-1 RNA qualitative assay tested with a WHO-approved HIV type 1 RNA standard in 16- and 32-member pools detected 100% of the pools (1,070 and 2,130 HIV-1 RNA copies/ml/pool, respectively), thus exceeding the FDA-required lower limit of detection. The Aptima test can be used to screen for acute-phase HIV infection.
authors.affiliation: Centers for Disease Control and Prevention, Division of HIV/AIDS Prevention, 1600 Clifton Road, Mailstop E-46, Atlanta, GA 30333, USA. sethridge@cdc.gov
authors.forename: Steven F
authors.initials: SF
authors.lastname: Ethridge
authors.forename: Clyde
authors.initials: C
authors.lastname: Hart
authors.forename: Debra L
authors.initials: DL
authors.lastname: Hanson
authors.forename: Monica M
authors.initials: MM
authors.lastname: Parker
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Sullivan
authors.forename: Berry
authors.initials: B
authors.lastname: Bennett
authors.forename: Petrice
authors.initials: P
authors.lastname: Stephens
authors.forename: Joslyn
authors.initials: J
authors.lastname: Hilliard
authors.forename: Pragna
authors.initials: P
authors.lastname: Patel
mesh_terms: D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral