title: Primary chlamydial infection correlates with high level of serum interleukin 12 (IL-12).
abstract: According to the existing data, cytokines actively participate in the pathogenesis of Chlamydia infection. However, the role of IL-12 in Chlamydiosis development has not been studied yet. The aim of our research is to define the role of IL-12 in the pathogenesis of the disease. The levels of IL-12, IL-4 and IFN-gamma were determined in 27 healthy and 56 Chlamydia infected individuals by using sandwich ELISA in vitro. Enzyme-linked immunosorbent assay, direct and indirect immunoflourescence were used for laboratory testing of Chlamydia. Statistical analysis of the results showed that the level of IL-12 was raised in the serum of some patients compared to the control group. Patients demonstrating high levels of IL-12 also had high levels of IFN-gamma and low to normal levels of IL-4. The high of IL-12 were mainly in patients with primary Chlamydia infection. Thus, IL-12 may participate in the development of the primary immune response to Chlamydia, in particular Th1 direction.
authors.affiliation: Medical Centre XXI Century, A. Tsereteli State University, Kutaisi, Georgia.
authors.forename: N
authors.initials: N
authors.lastname: Shavlakadze
authors.forename: B
authors.initials: B
authors.lastname: Gorgoshidze
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000302:isolation & purification; D016993:Chlamydophila pneumoniae / Q000276:immunology* / Q000302:isolation & purification; D002908:Chronic Disease; D005260:Female; D005846:Georgia (Republic); D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D008875:Middle Aged
chemical_list: D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma