Salidroside attenuates hypoxia-induced abnormal processing of amyloid precursor protein by decreasing BACE1 expression in SH-SY5Y cells.
Hypoxia which is mainly mediated by hypoxia-inducible factor 1 (HIF-1), can greatly contribute to the occurrence of Alzheimer's disease (AD) by increasing beta-site APP cleaving enzyme (BACE1) gene expression, protein level and beta-secretase activity, resulting in a significant generation of amyloid-beta (Abeta). Salidroside has been reported to have great neuroprotective effects. The aim of this study was to investigate the effects of salidroside on hypoxia-induced abnormal processing of the amyloid precursor protein (APP) in SH-SY5Y cells and its possible mechanism. Western blot analysis showed that 200muM of salidroside pretreatment significantly decreased BACE1 protein level and promoted the secretion of sAPPalpha in hypoxic condition. Salidroside had no effect on the level of APP, ADAM10 and ADAM17. ELISA analysis revealed that salidroside was able to inhibit the increase of beta-secretase activity and Abeta generation induced by hypoxia, with no effect on gamma-secretase activity. Notably, under hypoxia condition, mRNA of BACE1 and protein level of HIF-1alpha were decreased by salidroside pretreatment. These results demonstrated for the first time that salidroside was able to attenuate abnormal processing of amyloid precursor protein induced by hypoxia in SH-SY5Y cells, providing a new insight into prevention and treatment of Alzheimer's disease.
Li
Qing-Yun
QY
Lab of Neurodegenerative Diseases and Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences(SIBS), Chinese Academy of Sciences(CAS), and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Wang
Hong-Mei
HM
Wang
Zhi-Quan
ZQ
Ma
Jian-Fang
JF
Ding
Jian-Qing
JQ
Chen
Sheng-Di
SD
D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis*; D016564:Amyloid beta-Protein Precursor / Q000187:drug effects / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis*; D015153:Blotting, Western; D015687:Cell Hypoxia / Q000187:drug effects*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000096:biosynthesis*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016564:Amyloid beta-Protein Precursor; D005960:Glucosides; D051793:Hypoxia-Inducible Factor 1; D018696:Neuroprotective Agents; D010636:Phenols; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509282:Bace1 protein, mouse; C009172:rhodioloside