Spermine-graft-dextran non-covalent copolymer as coating material in separation of basic proteins and neurotransmitters by capillary electrophoresis.
Spermine-graft-dextran (Spe-g-Dex) copolymer was synthesized and used as a non-covalent coating for the separation of proteins and neurotransmitters by capillary electrophoresis. The coating was obtained via flushing the capillary with 1.0% Spe-g-Dex copolymer solution for 2min. Electroosmotic flow (EOF) was strongly suppressed, ranging from -1.60x10(-9) to 3.65x10(-9)m(2)V(-1)s(-1). Effect of experimental conditions, such as the copolymer concentration, the concentration and pH of the background electrolyte (BGE), on the Spe-g-Dex coating was investigated. Separation of lysozyme, cytochrome c, ribonuclease A and alpha-chymotrypsinogen yielded high separation efficiencies ranging from 141000 to 303000plates/m and recoveries from 85.4% to 98.3% at pH 4.0 (284.0mM sodium acetate-acetic acid buffer, I=50mM). Run-to-run repeatabilities and day-to-day, and capillary-to-capillary reproducibilities were all below 1.7%. In addition, Spe-g-Dex coating allowed the successful separation of five neurotransmitters, 5-hydroxytryptamine, dopamine, epinephrine, isoprenaline, dobuamine at pH 4.0 with high separation efficiencies of 290000-449000plates/m.
Wang
Ai-Jun
AJ
School of Chemistry and Environmental Science, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow and Huai Rivers Water Environment and Pollution Control, Key Laboratory of green chemical media and reactions, Henan Normal University, Xinxiang, Henan 453007, China. ajwangnju@gmail.com
Feng
Jiu-Ju
JJ
Dong
Wen-Ju
WJ
Lu
Ya-Hui
YH
Li
Zhong-Hua
ZH
Riekkola
Marja-Liisa
ML
D000818:Animals; D003911:Dextrans / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D013096:Spermine / Q000737:chemistry
D003911:Dextrans; D018377:Neurotransmitter Agents; D011108:Polymers; D011506:Proteins; D013096:Spermine