[Analysis of 4 clustered high risk acute flaccid paralysis cases in Shanxi Province in 2006].
OBJECTIVE
Analysis of epidemiology of 4 clustered high risk acute flaccid paralysis(AFP) cases reported by Shanxi province in 2006 and VP1 gene characteristic for type III poliovirus isolated from the four AFP cases.


METHODS
Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.


RESULTS
Three of four high risk AFP cases were suspected as vaccine associated paralysis poliomyelitis (VAPP), the onset date of them were close. VP1 sequencing of the four type III isolates revealed that the identity were 99.7%, 99.9%, 99.4% and 99.9% respectively compared with vaccine reference strain-BJOPV3. According to WHO criteria, the four isolates were identified as type III vaccine-related poliovirus. Phylogenetic analysis based on VP1 coding sequence showed that the four type III poliovirus were not related significantly. The type III poliovirus isolated from 3 suspected VAPP cases shared one nucleotide mutation at 2637 (C-->U), which result in the amino acid mutation from Val into Ala.


CONCLUSIONS
The improvement of laboratory surveillance for clustered high risk AFP cases should be strengthened so as to detect and prevent poliovirus circulation timely.
Yan
Dong-mei
DM
World Health Organization Western Pacific Region Regional Reference Polio Laboratory, State Key Laboratory for Molecular Virology & Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhang
Yong
Y
Wang
Dong-yan
DY
D019943:Amino Acid Substitution; D036022:Capsid Proteins / Q000235:genetics; D002681:China / Q000453:epidemiology; D006801:Humans; D007223:Infant; D010264:Paraplegia / Q000453:epidemiology* / Q000821:virology*; D010802:Phylogeny; D011051:Poliomyelitis / Q000453:epidemiology / Q000821:virology; D017955:Poliovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D017422:Sequence Analysis, DNA
D036022:Capsid Proteins; C442540:VP1 protein, Poliovirus