title: Kinetic evaluation of products inhibition to succinic acid producers Escherichia coli NZN111, AFP111, BL21, and Actinobacillus succinogenes 130Z T.
abstract: Succinic acid is one of the platform compounds and its production via natural feedstocks has drawn worldwide concerns. To evaluate the inhibitory effects of fermentation products on the growth of Actinobacillus succinogenes 130Z(T) and Escherichia coli NZN111, AFP111, BL21, fermentations with addition of individual products in medium were carried out. The cell growth was inhibited when the concentrations of formate, acetate, lactate, and succinate were at range of 8.8-17.6 g/L, 10-40 g/L, 9-18 g/L, and 10-80 g/L, respectively. For these two species of bacteria, E. coli was more resistant to acid products than A. succinogenes, while both endured succinate rather than by-products. As a result of end product inhibition, succinate production yield by A. succinogenes decreased from 1.11 to 0.49 g/g glucose. Logistic and Monod mathematical models were presented to simulate the inhibition kinetics. The Logistic model was found more suitable for describing the overall synergistic inhibitory effects.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.forename: Wangliang
authors.initials: W
authors.lastname: Li
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000188:Actinobacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005561:Formates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D019344:Lactic Acid / Q000494:pharmacology; D008954:Models, Biological; D013045:Species Specificity; D019802:Succinic Acid / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D005561:Formates; C030544:formic acid; D019344:Lactic Acid; D019802:Succinic Acid; D005947:Glucose; D019342:Acetic Acid