title: Antibiotic resistance of Gram negative bacilli strains isolated from the Intensive Care Unit in Fundeni Clinical Institute, Bucharest, Romania.
abstract: The aim of this study was to investigate the antibiotic resistance profile of 58 Gram negative bacilli strains (GNB): 36 non-fermentative GNB (NGNB), including 19 strains of Acinetobacter spp., 11 of Pseudomonas aeruginosa, 6 of Stenotrophomonas maltophilia and 22 enterobacterial strains (14 strains of KEHSs, 6 belonging to the group Proteus-Providencia and 2 Escherichia coli) isolated from nasal, pharyngeal exudates and also from bronchial secretions, from immuno-depressed patients admitted in the Intensive Care Unit of Fundeni Clinical Institute. METHHODS: the antibiotic susceptibility testing was performed according to CLSI 2009 recommendations and the production of beta-lactamases was investigated by ESBL chromogenic media, double disc diffusion test, ESBL E-test, Amp C E-test and MBL E-test.
  
  
  RESULTS
  68% of the enterobacterial strains produced extended-spectrum beta- lactamases (ESBL), 13.63% of them expressing simultaneously the Amp C enzyme. All enterobacterial strains were susceptible to carbapenems (Imipenem and Ertapenem). Metallo-beta lactamases production among NGNB strains with resistance to Imipenem was high (80%), these strains being also multi-resistant to the majority of tested antibiotics with the exception of colistin.
  
  
  CONCLUSIONS
  our results showed that the majority of the analyzed strains were multi-drug resistant. Antibiotic multi-resistance and the increasing number of severe infections caused by these strains are a major issue for ICU, where patients with severe diseases and destabilized physiological condition are often admitted.
authors.affiliation: Bacteriology Laboratory of Fundeni Clinical Institute, Sos. Fundeni 258, Bucharest, Romania.
authors.forename: Elvira
authors.initials: E
authors.lastname: Borcan
authors.forename: Camelia Mihaela
authors.initials: CM
authors.lastname: Ghiţă
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.forename: Luminiţa
authors.initials: L
authors.lastname: Măruţescu
authors.forename: Cătălina
authors.initials: C
authors.lastname: Isar
authors.forename: Veronica
authors.initials: V
authors.lastname: Lazăr
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D008826:Microbial Sensitivity Tests / Q000379:methods; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D012383:Romania / Q000453:epidemiology; D001618:beta-Lactamases / Q000145:classification / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases