Charge and size dependence of liposome diffusion in semidilute biopolymer solutions.
The diffusion of liposomes and PL/DNA complexes in mucin and collagen solutions, considered to model 'in vivo' colloidal gene delivery vector transport, is studied with FCS. The diffusion of defined liposomes is investigated as a function of particle size, surface charge, and the deviation from the Stokes-Einstein behavior. In all cases the self-diffusion coefficient decreases exponentially with polymer concentration. The same surface charge dependence of diffusion is observed in mucin for PL/DNA complexes with either positive or negative excess charge. Incubation of positively-charged PL/DNA complexes in a natural lung surfactant lipid increases the diffusion coefficients to almost the same as for the negatively-charged PL/DNA complexes.
Rusu
Laura
L
Ludwig-Maximilians-Universität, Fakultät für Physik und Center for NanoScience, Geschwister-Scholl-Platz 1, D-80539 München, Germany.
Lumma
Dirk
D
Rädler
Joachim O
JO
D003094:Collagen / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D018014:Gene Transfer Techniques; D008081:Liposomes / Q000737:chemistry*; D008954:Models, Biological; D009077:Mucins / Q000737:chemistry*; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry*
D008081:Liposomes; D009077:Mucins; D011107:Polylysine; D003094:Collagen; D004247:DNA