Design and synthesis of a highly selective fluorescent turn-on probe for thiol bioimaging in living cells.
A new fluorescent turn-on probe for the rapid optical sensing of thiols has been designed and synthesized. The probe displays high on/off signal ratios and high selectivity towards thiols. The potential of the probe as a biosensor for thiols was demonstrated by imaging of thiols in living cells.
Li
Xin
X
ZJU-ENS Joint Laboratory of Medicinal Chemistry, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Qian
Shijing
S
He
Qiaojun
Q
Yang
Bo
B
Li
Jia
J
Hu
Yongzhou
Y
D000818:Animals; D001896:Boron Compounds / Q000032:analysis / Q000138:chemical synthesis*; D019556:COS Cells; D002470:Cell Survival; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis*; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000032:analysis*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds