title: Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
abstract: IL-12 is a cytokine produced by dendritic cells (DC) and activates cytotoxic T cells and NK cells against tumors. We investigated the effect of IL-12 on DC. When peripheral blood monocytes were incubated with canine granulocyte-macrophage (GM)-CSF and IL-4, expression of MHC class II, CD1a, CD80 and CD86 was significantly elevated, but the cell morphology was that of immature DC. By adding canine IL-12 to the DC-inducing culture, expression of CD80 and CD1a was significantly enhanced, and the cell morphology shifted to that of mature DC. T cell stimulation by cultured cells was also significantly enhanced by the presence of IL-12. These results show that IL-12 significantly promotes the maturation and activation of canine DC following the induction of differentiation by GM-CSF and IL-4.
authors.affiliation: Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-ourai-kita, Izumisano City, Osaka 598-8531, Japan. sugiura@vet.osakafu-u.ac.jp
authors.forename: Kikuya
authors.initials: K
authors.lastname: Sugiura
authors.forename: Viskam
authors.initials: V
authors.lastname: Wijewardana
authors.forename: Mariko
authors.initials: M
authors.lastname: Fujimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Akazawa
authors.forename: Mana
authors.initials: M
authors.lastname: Yahata
authors.forename: Kai
authors.initials: K
authors.lastname: Mito
authors.forename: Shingo
authors.initials: S
authors.lastname: Hatoya
authors.forename: Norimitsu
authors.initials: N
authors.lastname: Inoue
authors.forename: Toshio
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004285:Dogs; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology
chemical_list: D018949:Antigens, CD1; D018122:B7-1 Antigen; C419393:CD1a antigen; D018664:Interleukin-12; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor