Performance of the Aptima HIV-1 RNA qualitative assay with 16- and 32-member specimen pools.
The Aptima HIV-1 RNA qualitative assay tested with a WHO-approved HIV type 1 RNA standard in 16- and 32-member pools detected 100% of the pools (1,070 and 2,130 HIV-1 RNA copies/ml/pool, respectively), thus exceeding the FDA-required lower limit of detection. The Aptima test can be used to screen for acute-phase HIV infection.
Ethridge
Steven F
SF
Centers for Disease Control and Prevention, Division of HIV/AIDS Prevention, 1600 Clifton Road, Mailstop E-46, Atlanta, GA 30333, USA. sethridge@cdc.gov
Hart
Clyde
C
Hanson
Debra L
DL
Parker
Monica M
MM
Sullivan
Timothy J
TJ
Bennett
Berry
B
Stephens
Petrice
P
Hilliard
Joslyn
J
Patel
Pragna
P
D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D012367:RNA, Viral