Expression of p53, MDM2, p21, heat shock protein 70, and HPV 16/18 E6 proteins in oral verrucous carcinoma and oral verrucous hyperplasia.
BACKGROUND
Oral verrucous hyperplasia is a precancerous lesion of oral verrucous carcinoma.


METHODS
This study used immunohistochemistry to examine the expression of p53, murine double minute 2 (MDM2), p21, heat shock protein 70 (HSP 70), and human papillomavirus (HPV) 16/18 E6 proteins in 48 oral verrucous carcinoma and 30 oral verrucous hyperplasia samples.


RESULTS
The mean labeling indices of p53, MDM2, p21, HSP 70, and HPV 16/18 E6 proteins in oral verrucous carcinoma samples were 21%, 31%, 7%, 17%, and 0.5%, respectively, and those in oral verrucous hyperplasia samples were 19%, 35%, 11%, 14%, and 0.3%, respectively.


CONCLUSIONS
Immunohistochemistry with the above-cited 5 biomarkers could not help differentiate oral verrucous hyperplasia from oral verrucous carcinoma. The low expression of p21 may partially explain abnormal epithelial overgrowth in both verrucous lesions. The pathogenesis of both verrucous lesions may be at least partially attributed to the overexpression of MDM2 protein and moderate expression of HSP 70 protein in both lesions.
Lin
Hung-Pin
HP
Graduate Institute of Clinical Dentistry and Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Wang
Yi-Ping
YP
Chiang
Chun-Pin
CP
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018289:Carcinoma, Verrucous / Q000378:metabolism* / Q000473:pathology; D002648:Child; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D052162:Human papillomavirus 16 / Q000378:metabolism; D052161:Human papillomavirus 18 / Q000378:metabolism; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000378:metabolism / Q000517:prevention & control; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D011230:Precancerous Conditions / Q000378:metabolism* / Q000473:pathology*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D055815:Young Adult; D054462:p21-Activated Kinases / Q000378:metabolism
D014408:Biomarkers, Tumor; D004279:DNA, Viral; D018840:HSP70 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53; D051736:Proto-Oncogene Proteins c-mdm2; D054462:p21-Activated Kinases