Phylogeographical particularity of the Mycobacterium tuberculosis Beijing family in South Korea based on international comparison with surrounding countries.
To understand the domestic population structure of Mycobacterium tuberculosis clinical isolates in the Republic of Korea, we genotypically analysed 80 isolates obtained from various geographical origins in the country. Of these, 64 (80.0 %) isolates were identified as Beijing family strains. It is particularly interesting that their phylogenetic classification, based on the ancient/modern separation and the presence/absence of the genomic region RD181, revealed a majority of the ancient (RD181+) subfamily in the population. The 15 loci of variable number of tandem repeat(s) of mycobacterial interspersed repetitive units (15-MIRU-VNTR) were also analysed. Combination with the previous VNTR data reported from surrounding countries revealed that the topology of the minimum spanning tree was linked tightly not to the geographical origins of the patients but to the phylogenetic characteristics of the isolates. These results show that the phylogeographical distribution of the M. tuberculosis Beijing family around far-eastern Asia could be estimated using international accumulation and comparison of VNTR genotyping data.
Kang
Hee Yoon
HY
Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Republic of Korea.
Wada
Takayuki
T
Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan.
Iwamoto
Tomotada
T
Department of Microbiology, Kobe Institute of Health, Kobe, Japan.
Maeda
Shinji
S
Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan.
Murase
Yoshiro
Y
Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan.
Kato
Seiya
S
Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan.
Kim
Hee Jin
HJ
Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Republic of Korea.
Park
Young Kil
YK
Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Republic of Korea.
D000328:Adult; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D005843:Geography; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011110:Polymorphism, Genetic; D056910:Republic of Korea / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
D004269:DNA, Bacterial