title: In vitro stimulation of human influenza-specific CD8+ T cells by dendritic cells pulsed with an influenza virus-like particle (VLP) vaccine.
abstract: The purpose of this in vitro study was to determine if a virus-like particle (VLP) influenza vaccine stimulated human CD8(+) T cells in a dendritic cell (DC): T cell co-culture system. VLP-pulsed DCs were co-cultured with autologous CD8(+) T cells from five donors. Functional CD8(+) T cells were detected via cell surface and intracellular cytokine staining. T cells from four of the five donors showed >or=2-fold increase over background in the % activated CD8(+) cells. These results indicate that the influenza VLP vaccine can stimulate CD8(+) T cells via DC antigen presentation, likely through the MHC-I pathway, thus broadening the immunological response induced by this promising influenza vaccine.
authors.affiliation: Novavax, Inc., Rockville, MD 20850, United States. Songhai@mail.nih.gov
authors.forename: Haifeng
authors.initials: H
authors.lastname: Song
authors.forename: Vaughan
authors.initials: V
authors.lastname: Wittman
authors.forename: Anthony
authors.initials: A
authors.lastname: Byers
authors.forename: Tenekua
authors.initials: T
authors.lastname: Tapia
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: William
authors.initials: W
authors.lastname: Warren
authors.forename: Penny
authors.initials: P
authors.lastname: Heaton
authors.forename: Kevin
authors.initials: K
authors.lastname: Connolly
mesh_terms: D000328:Adult; D017951:Antigen Presentation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D055815:Young Adult
chemical_list: D007252:Influenza Vaccines