[RAPD analysis on the germplasm resources of Baphicacanthus cusia].
OBJECTIVE
To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve.


METHODS
The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.


RESULTS
104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia. 422 DNA bands were amplified by 12 primers, 55.69% products were found to be polymorphic. Base on UPGMA cluster analysis, a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant, but not to geographic regions.


CONCLUSIONS
There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.
Huang
Yu-ji
YJ
Institute of Agrobiological resources, Fujian Academy of Agricultural Sciences/Medicinal Plant Research Center, Fuzhou 350003, China.
Chen
Jing-ying
JY
D027901:Acanthaceae / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D018744:DNA, Plant