title: Targeted exon sequencing by in-solution hybrid selection.
abstract: This unit describes a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in-solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool are reviewed, as are critical steps for performing the preparation and hybrid selection of an Illumina paired-end library. Critical parameters, performance metrics, and analysis workflow are discussed.
authors.affiliation: Broad Institute, Cambridge, Massachusetts, USA.
authors.forename: Brendan
authors.initials: B
authors.lastname: Blumenstiel
authors.forename: Kristian
authors.initials: K
authors.lastname: Cibulskis
authors.forename: Sheila
authors.initials: S
authors.lastname: Fisher
authors.forename: Matthew
authors.initials: M
authors.lastname: DeFelice
authors.forename: Andrew
authors.initials: A
authors.lastname: Barry
authors.forename: Tim
authors.initials: T
authors.lastname: Fennell
authors.forename: Justin
authors.initials: J
authors.lastname: Abreu
authors.forename: Brian
authors.initials: B
authors.lastname: Minie
authors.forename: Maura
authors.initials: M
authors.lastname: Costello
authors.forename: Geneva
authors.initials: G
authors.lastname: Young
authors.forename: Jared
authors.initials: J
authors.lastname: Maquire
authors.forename: Andrew
authors.initials: A
authors.lastname: Kernytsky
authors.forename: Alexandre
authors.initials: A
authors.lastname: Melnikov
authors.forename: Peter
authors.initials: P
authors.lastname: Rogov
authors.forename: Andreas
authors.initials: A
authors.lastname: Gnirke
authors.forename: Stacey
authors.initials: S
authors.lastname: Gabriel
mesh_terms: D005091:Exons / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012996:Solutions
chemical_list: D012996:Solutions