Cryptosporidium parvum: radiation-induced alteration of the oocyst proteome.
Cryptosporidium parvum is a waterborne protozoan parasite that is found intracellularly in host animals, including humans, and causes severe diarrhea, which can lead to the death of an immunocompromised individual. Previously, we found that this organism is highly radioresistant as it can productively infect mice after exposure to a 10-kGy dose of γ-radiation. To understand how C. parvum avoids radiation damage, we characterized its protein expression patterns 6, 24, and 48 h after a 10-kGy dose of γ-radiation using two-dimensional PAGE. The gels showed 10 silver-stained spots that increased or decreased in size following γ-irradiation. Five proteins contained in these spots were identified using MALDI-TOF MS peptide fingerprinting, and two of these showed an increase in expression after γ-irradiation. These proteins were identified by LC-MS/MS as proteasome subunit alpha type 4 (NTN hydrolase fold) and thioredoxin peroxidase-like protein. The roles of these two upregulated proteins as related to the radioresistance of C. parvum remain to be evaluated.
Lee
Soo-Ung
SU
Department of Environmental and Tropical Medicine, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea.
Joung
Migyo
M
Nam
Taekyoung
T
Park
Woo-Yoon
WY
Ji
Young-Hoon
YH
Yu
Jae-Ran
JR
D000818:Animals; D002853:Chromatography, Liquid; D016785:Cryptosporidium parvum / Q000737:chemistry / Q000528:radiation effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D033661:Oocysts / Q000737:chemistry / Q000528:radiation effects; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000737:chemistry / Q000528:radiation effects*; D015800:Protozoan Proteins / Q000737:chemistry / Q000528:radiation effects*; D016622:Silver Staining; D013047:Specific Pathogen-Free Organisms; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D020543:Proteome; D015800:Protozoan Proteins