Prevention effect in selenite-induced cataract in vivo and antioxidative effects in vitro of Crataegus pinnatifida leaves.
Cataract is the leading cause of blindness worldwide. It is a multifactorial disease primarily associated with oxidative stress produced by free radicals. The present study was undertaken to evaluate the anticataract potential of Crataegus pinnatifida (hawthorn tree) leaves extract in selenite-induced cataract in vivo and antioxidant effects in vitro. In vitro antioxidant assay of C. pinnatifida leaves extract on NO production inhibition, aldose reductase inhibition, and O(2)(-) radical scavenging activities gave the IC(50) of 98.3, 89.7, and 5.98 μg/mL, respectively. To characterize some major compounds in C. pinnatifida leaves extract, nine flavonoids were identified via LC-MS/MS qualitative analysis. Based on in vitro screening results, C. pinnatifida leaves extract eye drops in 0.1% hydroxypropyl methyl cellulose solution were prepared to evaluate the anticataract potential in vivo. Administration of C. pinnatifida leaves extract eye drops alternately three times a day in rat pups with selenite-induced oxidative stress significantly increased serum SOD and CAT activities, and tended to reduce MDA level compared with control group. The antioxidant enzyme SOD, CAT, and GSH activities in lens showed a significant increase. These results may be applied in the future for the prevention and treatment of cataracts.
Wang
Tao
T
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Nankai District, China.
Zhang
Peng
P
Zhao
Chunfeng
C
Zhang
Yi
Y
Liu
Hong
H
Hu
Limin
L
Gao
Xiumei
X
Zhang
Deqin
D
D000449:Aldehyde Reductase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002386:Cataract / Q000139:chemically induced / Q000188:drug therapy*; D027825:Crataegus; D005419:Flavonoids / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D009569:Nitric Oxide; D008315:Malondialdehyde; C007145:crataegus extract; D000449:Aldehyde Reductase; D013482:Superoxide Dismutase