title: The polymorphisms of bovine melanocortin-3 receptor pseudogene.
abstract: Mammalian melanocortin-3 receptor (MC3R) plays an important role in the central control of energy homeostasis, and several functional polymorphisms of mc3r have been detected. Interestingly, the bovine mc3r was a pseudogene, and its polymorphisms and function remain to be investigated. Single-strand conformation polymorphism (SSCP) showed 5, 2 and 3 genotypes in fragment F1, F2 and F3 of mc3r in seven cattle breeds, respectively. All genotypes revealed novel sequences. Three SNPs 657G>T, 756C>T, 822T>C were detected in fragment F1, five SNPs 1091T>C, 1133T>C, 1144C>T, 1259T>C and 1319G>A were detected in fragment F2, and two SNPs 1687G>A, 1860C>T were detected in F3. The SNPs in fragment F1 and F2 were located at exon 2. The five SNPs in fragment F2 demonstrated a tight linkage disequilibrium status. Variation detected here might have an impact on the function of bovine mc3r pseudogene.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling, Shaanxi 712100, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Zhang
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
authors.forename: X T
authors.initials: XT
authors.lastname: Fang
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D005838:Genotype; D011110:Polymorphism, Genetic; D044104:Receptor, Melanocortin, Type 3 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D044104:Receptor, Melanocortin, Type 3