title: The yeast GRASP Grh1 colocalizes with COPII and is dispensable for organizing the secretory pathway.
abstract: In mammalian cells, the 'Golgi reassembly and stacking protein' (GRASP) family has been implicated in Golgi stacking, but the broader functions of GRASP proteins are still unclear. The yeast Saccharomyces cerevisiae contains a single non-essential GRASP homolog called Grh1. However, Golgi cisternae in S. cerevisiae are not organized into stacks, so a possible structural role for Grh1 has been difficult to test. Here, we examined the localization and function of Grh1 in S. cerevisiae and in the related yeast Pichia pastoris, which has stacked Golgi cisternae. In agreement with earlier studies indicating that Grh1 interacts with coat protein II (COPII) vesicle coat proteins, we find that Grh1 colocalizes with COPII at transitional endoplasmic reticulum (tER) sites in both yeasts. Deletion of P. pastoris Grh1 had no obvious effect on the structure of tER-Golgi units. To test the role of S. cerevisiae Grh1, we exploited the observation that inhibiting ER export in S. cerevisiae generates enlarged tER sites that are often associated with the cis Golgi. This tER-Golgi association was preserved in the absence of Grh1. The combined data suggest that Grh1 acts early in the secretory pathway, but is dispensable for the organization of secretory compartments.
authors.affiliation: Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
authors.forename: Stephanie K
authors.initials: SK
authors.lastname: Levi
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Bhattacharyya
authors.forename: Rita L
authors.initials: RL
authors.lastname: Strack
authors.forename: Jotham R
authors.initials: JR
authors.lastname: Austin
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Glick
mesh_terms: D022181:COP-Coated Vesicles / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D055571:Secretory Pathway; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: C554600:Grh1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins