Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells.
Mesenchymal stem/stromal cells (MSCs) have been isolated from various tissues and utilized for an expanding number of therapies. The developmental pathways involved in producing MSCs and the phenotypic precursor/progenitor cells that give rise to human MSCs remain poorly defined. Human embryonic stem cells (hESCs) have the capability to generate functional hemato-endothelial cells and other mesoderm lineage cells. hESC-derived CD73(+) cells have been isolated and found to have similar phenotypic and functional characteristics as adult MSCs. Here we demonstrate hESC-derived CD34(+)CD73(-) cells can serve as MSC progenitor cells with the ability to differentiate into adipocytes, osteoblasts and chondrocytes. Additionally, gene array analysis of hESC-derived MSCs show substantially different gene expression compared to bone marrow (BM)-derived MSCs, especially with increased expression of pluripotent and multipotent stem cell and endothelial cell-associated genes. The isolation of functional MSCs from hESC-derived CD34(+)CD73(-) cells provides improved understanding of MSC development and utilization of pluripotent stem cells to produce MSCs suited for novel regenerative therapies.
Kopher
Ross A
RA
Department of Medicine and Stem Cell Institute, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN 55455, USA.
Penchev
Vesselin R
VR
Islam
Mohammad S
MS
Hill
Katherine L
KL
Khosla
Sundeep
S
Kaufman
Dan S
DS
D015720:5'-Nucleotidase / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006720:Homozygote; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis / Q000502:physiology; D040521:Subcutaneous Tissue; D013997:Time Factors
D018952:Antigens, CD34; D015720:5'-Nucleotidase