Expression profile of galectin-1 and galectin-3 molecules in different subtypes of chronic lymphocytic leukemia.
Galectin-1 (Gal-1) and galectin-3 (Gal-3) molecules are involved in many vital, biological, and pathological processes. In this study the expression pattern of these two molecules was investigated in leukemic cells from 85 Iranian chronic lymphocytic leukemia (CLL) patients, classified into indolent, progressive, mutated, and unmutated subtypes by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry methods. Our results showed significant downregulation of Gal-3, but not Gal-1, in CLL patients compared with normal subjects (p < .001). Higher representation of Gal-3 mRNA was observed in indolent patients compared with the progressive group (p < .05). Our findings imply a regulatory role for Gal-3 gene in initiation and/or progression of CLL.
Asgarian-Omran
Hossein
H
Department of Immunology, Tehran University of Medical Sciences, Iran.
Forghani
Parvin
P
Hojjat-Farsangi
Mohammad
M
Roohi
Azam
A
Sharifian
Ramazan Ali
RA
Razavi
Seyed Mohsen
SM
Jeddi-Tehrani
Mahmood
M
Rabbani
Hodjatallah
H
Shokri
Fazel
F
D000368:Aged; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D037483:Galectin 1 / Q000235:genetics* / Q000378:metabolism; D037502:Galectin 3 / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D037483:Galectin 1; D037502:Galectin 3; D012333:RNA, Messenger