title: Preparation of interconnected porous chitosan scaffolds by sodium acetate particulate leaching.
abstract: For tissue-engineering applications, a 3D porous chitosan scaffold was simply prepared from a mixture of acidic chitosan solution and sodium acetate particles as the porogen by a salt-leaching method. Differences in the porous structure in terms of pore morphology and interconnectivity between the salt-leached chitosan scaffold and phase-separated scaffold as the control were examined by using scanning electron microscopy, protein release and enzymatic degradation tests. A fibroblast (NIH-3T3) cell culture was performed for cell affinity evaluation. The chitosan scaffold prepared by salt-leaching showed good interconnectivity and improved mechanical properties. Furthermore, the chitosan scaffolds showed a high initial cell adhesion after 4 h cell culture and increased cell proliferation than the control. Thus, salt-leached chitosan scaffolds can be used for various tissue-engineering applications.
authors.affiliation: a National Core Research Center for Nanomedical Technology, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, South Korea.
authors.forename: Jin Ik
authors.initials: JI
authors.lastname: Lim
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Lee
authors.forename: Jeon-Soo
authors.initials: JS
authors.lastname: Shin
authors.forename: Kook-Jin
authors.initials: KJ
authors.lastname: Lim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D048271:Chitosan / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D064347:Microscopy, Electrochemical, Scanning; D041681:NIH 3T3 Cells; D016062:Porosity; D019346:Sodium Acetate / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D019346:Sodium Acetate; D048271:Chitosan