Breast cancer cells attract the migration of adipose tissue-derived stem cells via the PDGF-BB/PDGFR-beta signaling pathway.
The origin of vascular cells in tumors is unknown, but it is believed that tumors use cells from the host to build new vessels. To determine whether adipose tissue stem cells (ASCs) could be attracted by cancer cells, we performed migration assays in which ASCs were seeded on a transwell migration system top chamber and tumor-conditioned medium was placed in the bottom chamber. Our data showed that a significant number of ASCs migrated toward the tumor-conditioned medium (p<0.0001), and migration of human ASCs significantly (p<0.0001) increased in response to increased concentrations of recombinant PDGF-BB. In addition, neutralizing antibodies to PDGF receptor (PDGFR)-beta decreased migration of ASCs toward a breast cancer-conditioned medium to the level of serum-free control. These data suggest that tumor cell-derived PDGF-BB is an important factor in governing the microenvironment interaction between tumor cells and local tissue-resident stem cells.
Gehmert
Sebastian
S
Department of Molecular Pathology, The University of Texas MD Anderson Cancer Center, Unit 0951, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Gehmert
Sanga
S
Prantl
Lukas
L
Vykoukal
Jody
J
Alt
Eckhard
E
Song
Yao-Hua
YH
D000273:Adipose Tissue / Q000473:pathology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000077214:Becaplermin; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000473:pathology*
D057134:Antibodies, Neutralizing; D017077:Culture Media, Conditioned; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020797:Receptor, Platelet-Derived Growth Factor beta