title: The next-generation sequencing technology: a technology review and future perspective.
abstract: As one of the most powerful tools in biomedical research, DNA sequencing not only has been improving its productivity in an exponential growth rate but also been evolving into a new layout of technological territories toward engineering and physical disciplines over the past three decades. In this technical review, we look into technical characteristics of the next-gen sequencers and provide prospective insights into their future development and applications. We envisage that some of the emerging platforms are capable of supporting the $1000 genome and $100 genome goals if given a few years for technical maturation. We also suggest that scientists from China should play an active role in this campaign that will have profound impact on both scientific research and societal healthcare systems.
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China. joezhou@big.ac.cn.
authors.forename: XiaoGuang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China.
authors.forename: LuFeng
authors.initials: L
authors.lastname: Ren
authors.affiliation: Institute of Semiconductor, Chinese Academy of Sciences, Beijing, 100083, China.
authors.forename: YunTao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Semiconductor, Chinese Academy of Sciences, Beijing, 100083, China.
authors.forename: YuDe
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China. junyu@big.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
mesh_terms: D035843:Biomedical Research / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D002681:China; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005544:Forecasting; D016678:Genome / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods* / Q000639:trends
chemical_list: D004247:DNA