title: CK2 activity is modulated by growth rate in Saccharomyces cerevisiae.
abstract: CK2 is a highly conserved protein kinase controlling different cellular processes. It shows a higher activity in proliferating mammalian cells, in various types of cancer cell lines and tumors. The findings presented herein provide the first evidence of an in vivo modulation of CK2 activity, dependent on growth rate, in Saccharomyces cerevisiae. In fact, CK2 activity, assayed on nuclear extracts, is shown to increase in exponential growing batch cultures at faster growth rate, while localization of catalytic and regulatory subunits is not nutritionally modulated. Differences in intracellular CK2 activity of glucose- and ethanol-grown cells appear to depend on both increase in molecule number and k(cat). Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Farida
authors.initials: F
authors.lastname: Tripodi
authors.forename: Claudia
authors.initials: C
authors.lastname: Cirulli
authors.forename: Veronica
authors.initials: V
authors.lastname: Reghellin
authors.forename: Oriano
authors.initials: O
authors.lastname: Marin
authors.forename: Luca
authors.initials: L
authors.lastname: Brambilla
authors.forename: Maria Patrizia
authors.initials: MP
authors.lastname: Schiappelli
authors.forename: Danilo
authors.initials: D
authors.lastname: Porro
authors.forename: Marco
authors.initials: M
authors.lastname: Vanoni
authors.forename: Lilia
authors.initials: L
authors.lastname: Alberghina
authors.forename: Paola
authors.initials: P
authors.lastname: Coccetti
mesh_terms: D002244:Carbon / Q000378:metabolism; D047390:Casein Kinase II / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*
chemical_list: D002244:Carbon; D047390:Casein Kinase II