Occurrence of Biomphalaria cousini (Mollusca: Gastropoda) in Brazil and its susceptibility to Schistosoma mansoni (Platyhelminths: Trematoda).
In Brazil, there are three intermediate snail vectors and two potential hosts of Schistosoma mansoni. Previous studies showed three variant molecular profiles to B. amazonica and evidenced intraspecific variations using sequence data. In this context, the aim of this study was to verify whether such differences would correspond to either B. amazonica or B. cousini. The snails were morphologically identified; PCR-RFLP and sequencing were carried out. Besides, B. cousini were submitted to susceptibility experiments to S. mansoni. Noteworthy, morphological data of Brazilian specimens predominantly showed the morphology described for B. amazonica. Nevertheless, PCR-RFLP results exhibited three variant molecular profiles for the specimens previously identified as B. amazonica and the phylogenetic analyses showed two groups one to B. amazonica and another to B. cousini. Furthermore, B. cousini showed to be susceptible to S. mansoni. These results confirm the occurrence of B. cousini in Brazil and points to the risk of introduction of schistosomiasis mansoni into new areas.
Teodoro
Tatiana Maria
TM
Laboratório de Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, Minas Gerais, Brazil. tatianateo@cpqrr.fiocruz.br
Janotti-Passos
Liana Konovalloff
LK
Carvalho
Omar dos Santos
Odos S
Caldeira
Roberta Lima
RL
D000818:Animals; D001700:Biomphalaria / Q000145:classification / Q000235:genetics* / Q000469:parasitology*; D001938:Brazil; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012550:Schistosoma mansoni; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer