Construction of tissue-engineered cartilage using human placenta-derived stem cells.
Human placenta-derived stem cells (hPDSCs) were isolated by trypsinization and further induced into cartilage cells in vitro. The engineered cartilage was constructed by combining hPDSCs with collagen sponge and the cartilage formation was observed by implantation into nude mice. Results showed that hPDSCs featured mesenchymal stem cells and maintained proliferation in vitro for over 30 passages while remaining undifferentiated. All results indicated that hPDSCs have the potential to differentiate into functional cartilage cells in vitro when combined with collagen sponge, which provided experimental evidence for prospective clinical application.
Liu
DaShun
D
Department of ENT, Xijing Hospital, the Foruth Military Medical University of PLA, Xi'an, 710032, China.
Hui
HuangFu
H
Chai
XiangBin
X
Wang
BinQuan
B
Qiu
JianHua
J
D000818:Animals; D002356:Cartilage / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003094:Collagen; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
D003094:Collagen