[Research on callus induction and plantlet regeneration of Prunella vulgaris].
OBJECTIVE
To study the conditions of callus induction and plantlet regeneration of Prunella vulgaris.


METHODS
By using the orthogonal experiment design, different explants, sucrose, plant growth substances and their ratio for callus induction and differentiation were optimized.


CONCLUSIONS
The inductivity of leaves was the highest, followed by stems, but callus of the leafstalks could not be induced. The main factor of affecting the formation of callus was 6-BA. The optimal medium for callus induction was MS + 6-BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + 2,4-D 0.5 mg x L(-1) + sucrose 3%. The optimal medium for callus differentiation was 1/2 MS + 6-BA 3.25 mg x L(-1) + NAA 1.25 mg x L(-1) + sucrose 2%. The optimal medium for rooting was MS + IBA 1.0 mg x L(-1) + sucrose 3%.
Sheng
Wei
W
Anhui Key Laboratory of Plant Resources and Biology, School of Life Science, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
Chang
Li
L
Zhang
Aimin
A
Xue
Jianping
J
Liu
Yuling
Y
D003470:Culture Media / Q000737:chemistry; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D031347:Prunella / Q000187:drug effects* / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects*; D013997:Time Factors
D003470:Culture Media