title: Magnetic resonance and fluorescence imaging of doxorubicin-loaded nanoparticles using a novel in vivo model.
abstract: We report here the in vivo combined-modality imaging of multifunctional drug delivery nanoparticles. These dextran core-based stealth liposomal nanoparticles (nanosomes) contained doxorubicin, iron oxide for magnetic resonance imaging (MRI) contrast, and BODIPY for fluorescence. The particles were long-lived in vivo because of surface decoration with polyethylene glycol and the incorporation of acetylated lipids that were ultraviolet cross-linked for physical stability. We developed a rodent dorsal skinfold window chamber that facilitated both MRI and non-invasive optical imaging of nanoparticle accumulation in the same tumors. Chamber tumors were genetically labeled with DsRed-2, which enabled co-localization of the MR images, the red fluorescence of the tumor, and the blue fluorescence of the nanoparticles. The nanoparticle design and MR imaging developed with the window chamber were then extended to orthotopic pancreatic tumors expressing DsRed-2. The tumors were MR-imaged using iron oxide-dextran liposomes and by fluorescence to demonstrate the deep imaging capability of these nanoparticles.
authors.affiliation: Department of Nanoengineering, University of California San Diego, San Diego, California, USA.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Erten
authors.forename: Wolf
authors.initials: W
authors.lastname: Wrasidlo
authors.forename: Miriam
authors.initials: M
authors.lastname: Scadeng
authors.forename: Sadik
authors.initials: S
authors.lastname: Esener
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Milan
authors.initials: M
authors.lastname: Makale
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use*; D005453:Fluorescence; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy
chemical_list: D008081:Liposomes; D004317:Doxorubicin