Ultrastructural effects of albendazole on the body wall of Gnathostoma spinigerum third stage larvae.
This study investigated the effects of albendazole (ABZ) on the ultrastructure of Gnathostoma spinigerum advanced third-stage larvae. Two groups of experimentally infected mice received 60 or 90 mg/kg ABZ orally once a day for 21 consecutive days. Both groups had damage to the body walls of the worms, especially to the non-contractile part of the muscular layer. The severity of the damage was dose related, the higher the dose, the greater the damage. The body wall of the ABZ treated larvae demonstrated a decrease in the number of mitochondria in the non-contractile muscular part, especially in the internal surface of the sarcolemma. Some mitochondria developed large vacuoles, and became distorted and degenerated. The nuclei degenerated and had irregular shapes and the number of glycogen granules decreased. The present study demonstrates the structural damage induced by the toxic effects of ABZ and increases our knowledge of the mechanism of action of ABZ against G. spinigerum.
Arunyanart
Channarong
C
Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Kanla
Pipatphong
P
Chaichun
Amnart
A
Intapan
Pewpan M
PM
Maleewong
Wanchai
W
D015766:Albendazole / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006039:Gnathostoma / Q000187:drug effects* / Q000648:ultrastructure; D007814:Larva / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009349:Nematode Infections / Q000188:drug therapy*; D011897:Random Allocation; D018709:Statistics, Nonparametric
D000871:Anthelmintics; D015766:Albendazole