title: Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples.
abstract: A rapid (less than 10 min), qualitative and semi-quantitative immunochromatography using colloidal gold-antibody probe was successfully developed and applied in determination of chlorpyrifos-methyl (a wide-spectrum organophosphorus pesticide) in water samples. The qualitative detection limit of chlorpyrifos-methyl was determined as 0.6 μg ml(-1) by using immunochromatography. In the semi-quantitative experiment, the detection results of chlorpyrifos-methyl were scanned by a membrane strip reader, and a detection curve representing the scanned data average was obtained. After conversion, it was observed that in the range of 50-12,150 ng ml(-1), the graph between logit(B/B(0)) and logarithm of concentration of chlorpyrifos-methyl was linear, from which, the regression equation (y =-2.5229 x +7.5951, R(2)=0.9889) and IC(50) value (1024.39 ng ml(-1)) was obtained, respectively. Meanwhile, the detection limit was calculated as 132.91 ng ml(-1) by the extrapolation of B(0)-2SD. In addition, the cross-reactivities were less than 1% with tested analog compounds and regarded as negligible. The recoveries obtained by standard chlorpyrifos-methyl addition to water samples were 102.5-107.6%. Overall, to our knowledge, this is the first report of qualitative and semi-quantitative detection of chlorpyrifos-methyl by immunochromatography.
authors.affiliation: Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Chinese Ministry of Agriculture, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Hua
authors.forename: Guoliang
authors.initials: G
authors.lastname: Qian
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.forename: Baishi
authors.initials: B
authors.lastname: Hu
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Fan
authors.forename: Na
authors.initials: N
authors.lastname: Qin
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
mesh_terms: D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D010575:Pesticides / Q000032:analysis*; D011934:Reagent Strips; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D010575:Pesticides; D011934:Reagent Strips; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos; C007031:chlorpyrifos-methyl