title: A method to site-specifically introduce methyllysine into proteins in E. coli.
abstract: A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Hui-Wang
authors.initials: HW
authors.lastname: Ai
authors.forename: Jae Wook
authors.initials: JW
authors.lastname: Lee
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D008239:Lysine / Q000031:analogs & derivatives* / Q000235:genetics; D009154:Mutation; D012343:RNA, Transfer / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; C049907:2-methyllysine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine