Rapid identification of Iranian Acinetobacter baumannii strains by single PCR assay using BLA oxa-51 -like carbapenemase and evaluation of the antimicrobial resistance profiles of the isolates.
The rapid identification of relevant bacterial pathogens is of utmost importance in clinical settings. The aim of this study was to test a rapid identification technique for A. baumannii strains from Tehran Hospitals and to determine the antibiotic resistance profiles of the isolates. A hundred strains of Acinetobacter spp. grown from clinical specimens were identified as A. baumannii by conventional methods. Using PCR a bla OXA-51 -like gene was detected in all A. baumannii isolates but not in other species of acinetobacter. More than half of the isolates proved resistant to a variety of antibiotics by the disk diffusion technique. The rate of resistance to gentamicin, imipenem, ampicillin-sulbactam and amikacin was determined to be 45%, 53%, 62% and 62%, respectively. Moreover, most isolates (more than 90%) showed resistance to cephalosporins. This study shows that the demonstration of the bla OXA-51-like gene is a reliable and rapid way for the presumptive identification of A. baumannii and reveals that the rate of antibiotic resistance is high in Iranian A. baumannii isolates to a variety of antibiotics.
Akbari
Mahdi
M
Department of Microbiology, School of Medicine, University of Shahed, Tehran, Iran.
Mahdi
Akbari
A
Niakan
Mohammad
M
Mohammad
Niakan
N
Taherikalani
Morovat
M
Morovat
Taherikalani
T
Feizabadi
Mohammad Mehdi
MM
Mhammad-Mahdi
Feizabadi
F
Azadi
Namam Ali
NA
Namam-Ali
Azadi
A
Soroush
Setareh
S
Setareh
Soroush
S
Emaneini
Mohammad
M
Mohammad
Emaneini
E
Abdolkarimi
Amir
A
Amir
Abdolkarimi
A
Maleki
Abbas
A
Abbas
Maleki
M
Hematian
Ali
A
Ali
Hematian
H
D040981:Acinetobacter baumannii / Q000187:drug effects / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases