title: [CAP59 gene amplification in Cryptococcus neoformans and Cryptococcus gattii directly from a yeast suspension].
abstract: Cryptococcus is an encapsulated yeast of class Basidiomycetes, etiologic agent of cryptococcosis. Cryptococcosis is one of the most common opportunistic infections in immunosuppressed patients, although it can affect immunocompetent individuals. In recent years, the identification of medically important fungal species has been achieved through the amplification of specific regions or genes of fungal DNA by polymerase chain reaction (PCR). The CAP59 gene is involved in the synthesis of the capsule in Cryptococcus neoformans and Cryptococcus gattii, and is useful in the molecular identification of serotypes. In this research, we use yeasts of different serotypes from collection strains and C. neoformans isolates recovered from patients with cryptococcosis. A standardized yeast suspension from different Cryptococcus isolates as template allowed CAP59 gene amplification. This procedure was quick, simple, and inexpensive and required no PCR steps. This is important for taxonomic studies in laboratories with implemented molecular biology tools.
authors.affiliation: Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Siachoque
authors.forename: V M
authors.initials: VM
authors.lastname: Jewtuchowicz
authors.forename: C
authors.initials: C
authors.lastname: Iovannitti
authors.forename: M T
authors.initials: MT
authors.lastname: Mujica
mesh_terms: D003453:Cryptococcosis / Q000382:microbiology; D056285:Cryptococcus gattii / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D009172:Mycology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods; D013535:Suspensions
chemical_list: C087781:CAP59 protein, Cryptococcus neoformans; D004271:DNA, Fungal; D005656:Fungal Proteins; D013535:Suspensions