title: Evaluation of hypoxic tissue dynamics with 18F-FMISO PET in a rat model of permanent cerebral ischemia.
abstract: OBJECTIVE
  [¹⁸F]Fluoromisonidazole (¹⁸F-FMISO) is a nitroimidazole derivative that has been proposed as a positron emission tomography (PET) radiotracer to detect hypoxic tissue in vivo. This compound accumulates in hypoxic but viable tissue and may be a good candidate for evaluating the ischemic penumbra. We evaluated the time course of ¹⁸F-FMISO uptake using PET in a rat model of permanent cerebral ischemia and the correlation with histological changes.
  
  
  METHODS
  Rats (n = 14) were subjected to permanent ischemia by intraluminal occlusion of the middle cerebral artery in order to assess by PET the uptake of ¹⁸F-FMISO at various times over 24 h following ischemia. The PET results were compared to histological changes with Nissl and 2,3,5 triphenyltetrazolium chloride staining.
  
  
  RESULTS
  Elevated uptake of ¹⁸F-FMISO was detected in the infarcted area up to 8 h after occlusion but was no longer detected at 24 h, a time point coincident with pan necrosis of the tissue.
  
  
  CONCLUSIONS
  Our findings suggest that salvageable tissue persists for up to 8 h in this rat model of brain ischemia. We propose ¹⁸F-FMISO PET as a tool for evaluating the ischemic penumbra after cerebral ischemia.
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain. srojas@crccorp.es.
authors.forename: Santiago
authors.initials: S
authors.lastname: Rojas
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: José Raul
authors.initials: JR
authors.lastname: Herance
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Abad
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Jiménez
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
authors.forename: Deborah
authors.initials: D
authors.lastname: Pareto
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Ruiz
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Èlia
authors.initials: È
authors.lastname: Torrent
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Francisca P
authors.initials: FP
authors.lastname: Figueiras
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Foteini
authors.initials: F
authors.lastname: Popota
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Fernández-Soriano
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institut d'Investigacions Biomèdiques de Barcelona (IIBB)-Consejo Superior de Investigaciones Científicas (CSIC), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Planas
authors.affiliation: Institut d'Alta Tecnologia, Parc de Recerca Biomèdica de Barcelona (IAT-PRBB) CRC Corporació Sanitària, C/Dr Aiguader 88, 08003, Barcelona, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Gispert
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000473:pathology*; D015687:Cell Hypoxia; D004195:Disease Models, Animal; D008297:Male; D008920:Misonidazole / Q000031:analogs & derivatives*; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: C031843:fluoromisonidazole; D008920:Misonidazole