Effects of QWBZP on T-cell subsets and their cytokines in intestinal mucosa of HRV infection suckling mice.
OBJECTIVE
Qiwei Baizhu Powder (QWBZP) is a traditional herbal prescription that has been used traditionally for the treatment of infantile diarrhea, including the infantile diarrhea caused by Human Rotavirus (HRV). In this study, we investigated the pharmacological activity of QWBZP extract.


METHODS
NIH suckling mice with HRV induced diarrhea were used. Density of CD3(+), CD4(+) and CD8(+) T cells, mRNA expression of IL-2, IFN-gamma, IL-4 and IL-10 in intestinal mucosa epithelial cells were assayed.


RESULTS
QWBZP extract promoted the expressions of mRNA of IL-2, IL-4, IL-10 and IFN-gamma in intestinal mucosa epithelial cells. Also, we found that the density of CD8(+) cells in intestinal mucosa epithelial cells was significantly lower in QWBZP group than in Model group, while the density of CD8(+) cells was significantly higher in QWBZP group than in Model group.


CONCLUSIONS
These data suggest that QWBZP extract may exhibit antiviral effects through modulating the densities of T-cell subsets and the expressions of their cytokines in small intestinal mucosa epithelial cells.
Wu
Can-rong
CR
Department of Etiology and Immunology, Hunan University of TCM, Shaoshan Road, Changsha, Hunan 410007, PR China. wucanrong2008@yahoo.com.cn
Jiang
Xiao
X
He
Shuang-teng
ST
Yang
Bo
B
Hu
Jian-zhong
JZ
Cai
Rui
R
D000818:Animals; D000833:Animals, Suckling; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D018514:Plant Structures; D011247:Pregnancy; D011897:Random Allocation; D012401:Rotavirus; D012400:Rotavirus Infections / Q000188:drug therapy* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology
D016207:Cytokines; D004365:Drugs, Chinese Herbal