title: Antagomir dependent microRNA-205 reduction enhances adhesion ability of human corneal epithelial keratinocytes.
abstract: OBJECTIVE
  To investigate the effect of microRNA-205 reduction by antagomirs on adhesion ability of normal human corneal epithelial keratinocytes (NHCEKs).
  
  
  METHODS
  Antagomir-205, complementary and inhibitory to microRNA-205, was used to suppress endogenous microRNA-205 in NHCEKs. The adhesion ability of treated NHCEKs was then assessed by cell adhesion assay. Immunoblot and immunohistochemistry were conducted to determine the level of two focal adhesion-related proteins, focal adhesion kinase (FAK) and paxillin (Pax). Phalloidin staining was performed to measure the level of filamentous actin in antagomir-treated NHCEKs.
  
  
  RESULTS
  Antagomir-205 markedly reduced the level of microRNA-205 in NHCEKs and significantly enhanced adhesion ability of NHCEKs (P<0.01). Further protein analysis validated that inhibition of microRNA-205 increased the number of phosphorylated FAK and phosphorylated Pax, and decreased filamentous actin.
  
  
  CONCLUSIONS
  Our findings suggest that microRNA-205 has down-regulating effect on cell motility in NHCEKs.
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academyof Medical Sciences & Peking Union Medical College, Beijing 100005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Jun-wu
authors.initials: JW
authors.lastname: Zhang
authors.forename: Robert M
authors.initials: RM
authors.lastname: Lavker
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D002448:Cell Adhesion / Q000235:genetics*; D002478:Cells, Cultured; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D035683:MicroRNAs / Q000037:antagonists & inhibitors*; D015345:Oligonucleotide Probes
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes