Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis.
Nicotinamide adenine dinucleotide (NAD) and its derivative nicotinamide adenine dinucleotide phosphate (NADP) are indispensable co-factors in broad-spectrum metabolic events for the maintenance of cellular homeostasis in all living organisms. In this study, the cellular expression levels of NAD biosynthesis genes in Arabidopsis were investigated. A very high expression of nicotinate/nicotinamide mononucleotide adenyltransferase (NMNAT) was observed in the differentiated stomatal guard cells of the leaf surface. Transcriptional analysis confirmed that several genes in the biosynthesis pathway were also highly expressed in stomatal guard cells. In fact, NAD and NADP metabolisms were investigated during stomatal movement. Importantly, the generation of phytohormone ABA-induced reactive oxygen species, which acts as a signal for stomatal closure, was accompanied by markedly decreased levels of NAD. The ABA-induced oxidative stress caused stomatal cell death in the nmnat mutant. Furthermore, stomata partially lost their ability to close leading to drought susceptibility. The stomata were less responsive to opening cues as well. These results indicate that NAD biosynthesis is involved in protecting guard cells from ABA-induced local oxidative stress via the regulation of NMNAT activity. In this study, it is demonstrated that NMNAT is essential for the maintenance of NAD homeostasis enabling sustainable stomatal movement.
Hashida
Shin-nosuke
SN
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. shashida@criepi.denken.or.jp
Itami
Taketo
T
Takahashi
Hideyuki
H
Takahara
Kentaro
K
Nagano
Minoru
M
Kawai-Yamada
Maki
M
Shoji
Kazuhiro
K
Goto
Fumiyuki
F
Yoshihara
Toshihiro
T
Uchimiya
Hirofumi
H
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D003681:Dehydration; D018506:Gene Expression Regulation, Plant; D009243:NAD / Q000096:biosynthesis* / Q000378:metabolism; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D054046:Plant Stomata / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013312:Stress, Physiological / Q000502:physiology
D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D009243:NAD; D000040:Abscisic Acid; D009612:Nicotinamide-Nucleotide Adenylyltransferase