Effect of mitogen lectin on lymphocyte or brain cortex cell activation.
OBJECTIVE
We studied a) mitogen lectin (PHA) evoked changes of Na+/K+-ATPase activity in functionally different lymphocytes or brain cortex cells and b) quantitative relationship between PHA- evoked early enzyme activation and late lymphocyte proliferation were analyzed.


METHODS
We performed biochemical analyses of Pi released from ATP by Na+/K+-ATPase activity. Lymphocyte proliferation was assayed by 3H-thymidine incorporation.


RESULTS
We demonstrated PHA stimulated Na+/K+-ATPase activity of mouse spleen lymphocytes or freshly isolated brain cortex cells. Besides this, we estimated high stimulation of Na+/K+-ATPase activity and subsequent late 3H-thymidine incorporation into pig lymphocytes as both PHA dose and K+ ion concentration dependent.


CONCLUSIONS
Thus, early PHA dose-dependent stimulation of Na+/K+-ATPase activity is a more general response in different animal species and functionally different cells. We measured both cell type- and PHA-dose dependent enzyme activity stimulation. We can suggest that intensity of early PHA induced Na+/K+-ATPase activation could be in relationship to subsequent elevated level of T lymphocyte proliferation. The Na+/K+-ATPase can be a part of mitogen lectin evoked signal transduction mechanisms.
Kovaru
Hana
H
1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. hkova@lf1.cuni.cz
Kovaru
Frantisek
F
Pav
Marek
M
Dvorak
Rudolf
R
Palikova
Miloslava
M
Raboch
Jiri
J
Zidková
Jarmila
J
D000818:Animals; D018929:Cell Culture Techniques; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism
D008934:Mitogens; D010835:Phytohemagglutinins; C101135:phytohemagglutinin L protein, Phaseolus vulgaris; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium