title: Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer.
abstract: Omega-3(omega-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of omega-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. pandengke@yahoo.com.cn
authors.forename: DengKe
authors.initials: D
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: YanRong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chong
authors.initials: C
authors.lastname: Feng
authors.forename: Chuan
authors.initials: C
authors.lastname: Long
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Wan
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: AiXing
authors.initials: A
authors.lastname: Lin
authors.forename: EnQiu
authors.initials: E
authors.lastname: Dong
authors.forename: ShuChen
authors.initials: S
authors.lastname: Wang
authors.forename: HouGang
authors.initials: H
authors.lastname: Xu
authors.forename: HongXing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002681:China; D004171:Diploidy; D004624:Embryo Transfer; D044943:Fatty Acid Desaturases / Q000235:genetics*; D015525:Fatty Acids, Omega-3 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D053652:Nuclear Transfer Techniques; D034421:Sus scrofa / Q000235:genetics*; D014162:Transfection
chemical_list: D015525:Fatty Acids, Omega-3; D044943:Fatty Acid Desaturases; C078565:omega-3 fatty acid desaturase