Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing.
BACKGROUND
OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality.


RESULTS
We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions.


CONCLUSIONS
A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.
Azulay
David-Olivier D
DO
Pfizer Global Research and Development, Sandwich, UK. david-olivier.azulay@pfizer.com
Neubert
Hendrik
H
Ocaña
Mireia Fernández
MF
D002457:Cell Extracts; D002460:Cell Line; D005591:Chemical Fractionation; D057225:Data Mining; D006801:Humans; D007525:Isoelectric Focusing; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D012984:Software
D002457:Cell Extracts; D010455:Peptides