title: Visualization of kinase activity with FRET-based activity biosensors.
abstract: Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy.
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Charlene
authors.initials: C
authors.lastname: Depry
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D005057:Eukaryotic Cells / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008322:Mammals; D008856:Microscopy, Fluorescence; D010770:Phosphotransferases / Q000032:analysis*
chemical_list: D010770:Phosphotransferases