title: Outer membrane vesicles derived from Escherichia coli induce systemic inflammatory response syndrome.
abstract: Sepsis, characterized by a systemic inflammatory state that is usually related to Gram-negative bacterial infection, is a leading cause of death worldwide. Although the annual incidence of sepsis is still rising, the exact cause of Gram-negative bacteria-associated sepsis is not clear. Outer membrane vesicles (OMVs), constitutively secreted from Gram-negative bacteria, are nano-sized spherical bilayered proteolipids. Using a mouse model, we showed that intraperitoneal injection of OMVs derived from intestinal Escherichia coli induced lethality. Furthermore, OMVs induced host responses which resemble a clinically relevant condition like sepsis that was characterized by piloerection, eye exudates, hypothermia, tachypnea, leukopenia, disseminated intravascular coagulation, dysfunction of the lungs, hypotension, and systemic induction of tumor necrosis factor-alpha and interleukin-6. Our study revealed a previously unidentified causative microbial signal in the pathogenesis of sepsis, suggesting OMVs as a new therapeutic target to prevent and/or treat severe sepsis caused by Gram-negative bacterial infection.
authors.affiliation: Division of Molecular and Life Sciences, Department of Life Science, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Kyong-Su
authors.initials: KS
authors.lastname: Park
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Choi
authors.forename: You-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Bok Sil
authors.initials: BS
authors.lastname: Hong
authors.forename: Oh Youn
authors.initials: OY
authors.lastname: Kim
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Chang Min
authors.initials: CM
authors.lastname: Yoon
authors.forename: Gou-Young
authors.initials: GY
authors.lastname: Koh
authors.forename: Yoon-Keun
authors.initials: YK
authors.lastname: Kim
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology*; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D011510:Proteolipids / Q000502:physiology*; D018746:Systemic Inflammatory Response Syndrome / Q000382:microbiology*
chemical_list: D011510:Proteolipids