Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar.
OBJECTIVE
Orthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.


METHODS
Tiny fragments of surgical tumors were implanted into the mice brain with a trocar system. Immunohistochemistry was performed to detect brain tumor stem cells among glioblastoma tissues, including both the original and resulting ones with monoclonal antibody against CD133.


RESULTS
Besides the constant high take rates in both models; brain transplants perfectly resembled their original tumors in biological behaviors. The brain tumor stem cells, positively stained with CD133 were found, though not frequently, in both original and resulting glioblastoma tissues.


CONCLUSIONS
Orthotopic model established with a trocar system is effective and injection of tumor tissues containing stem cells promise the forming of new tumor mass when grafted.
Fei
Xi Feng
XF
Neurosurgical Department, Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, 215006, Suzhou, China.
Zhang
Quan Bin
QB
Dong
Jun
J
Diao
Yi
Y
Wang
Zhi Min
ZM
Li
Ru Jun
RJ
Wu
Zi Cheng
ZC
Wang
Ai Dong
AD
Lan
Qing
Q
Zhang
Shi Ming
SM
Huang
Qiang
Q
D000071916:AC133 Antigen; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001932:Brain Neoplasms / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D005909:Glioblastoma / Q000473:pathology* / Q000601:surgery; D006023:Glycoproteins / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary* / Q000601:surgery; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000473:pathology*; D010455:Peptides / Q000276:immunology; D013525:Surgical Instruments; D015996:Survival Rate; D023041:Xenograft Model Antitumor Assays
D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; C000606024:Prom1 protein, mouse