Pro-inflammatory cytokine and chemokine mRNA blood level in multiple sclerosis is related to treatment response and interferon-beta dose.
Of 37 multiple sclerosis patients, 19 suboptimal responders were randomized to 375 (n=12) or 250µg (n=7) interferon (IFN)-β-1b. mRNA levels of 23 cytokines, chemokines, and chemokine receptors were quantified by TaqMan low-density array (TLDA) real-time polymerase chain reaction. Better treatment responses or increased IFN-β doses were associated with elevated IL-10 and TGF-β and decreased CXCL10, IL-18, IFN-γ, and TNF-α transcript levels. Adjusting for dose, poor treatment responses resulted in a 4-fold increase in CXCL10 and IFN-γ expression (Mantel-Haenszel RR=3.74, p<0.0001). CXCL10 and IFN-γ mRNA levels were reliable indicators of treatment response. TLDA can be used to tailor IFN-β-1b therapy.
Cucci
Angele
A
University Division of Neurology, Facolta' San Luigi Gonzaga, Universita' di Torino, Ospedale Universitario San Luigi Gonzaga, Regione Gonzole, 10, I-10043 Orbassano, Turin, Italy.
Barbero
Pierangelo
P
Clerico
Marinella
M
Ferrero
Bruno
B
Versino
Elisabetta
E
Contessa
Giulia
G
Demercanti
Stefania
S
Viglietta
Emanuela
E
Di Liberto
Alessandra
A
Vai
Alessandra Giai
AG
Durelli
Luca
L
D000328:Adult; D016207:Cytokines / Q000097:blood* / Q000145:classification / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology / Q000627:therapeutic use*; D008137:Longitudinal Studies; D008297:Male; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology*; D012333:RNA, Messenger / Q000097:blood*; D055815:Young Adult
D016207:Cytokines; D012333:RNA, Messenger; D016899:Interferon-beta