title: Biodiesel production with immobilized lipase: A review.
abstract: Fatty acid alkyl esters, also called biodiesel, are environmentally friendly and show great potential as an alternative liquid fuel. Biodiesel is produced by transesterification of oils or fats with chemical catalysts or lipase. Immobilized lipase as the biocatalyst draws high attention because that process is "greener". This article reviews the current status of biodiesel production with immobilized lipase, including various lipases, immobilization methods, various feedstocks, lipase inactivation caused by short chain alcohols and large scale industrialization. Adsorption is still the most widely employed method for lipase immobilization. There are two kinds of lipase used most frequently especially for large scale industrialization. One is Candida antartica lipase immobilized on acrylic resin, and the other is Candida sp. 99-125 lipase immobilized on inexpensive textile membranes. However, to further reduce the cost of biodiesel production, new immobilization techniques with higher activity and stability still need to be explored.
authors.affiliation: Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, PR China. twtan@mail.buct.edu.cn
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.forename: Jike
authors.initials: J
authors.lastname: Lu
authors.forename: Kaili
authors.initials: K
authors.lastname: Nie
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D056804:Biofuels; D019149:Bioreactors; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica