title: Visible light-induced crosslinkable gelatin.
abstract: A novel visible light-crosslinkable porcine gelatin was prepared for gelation and micropatterning. The preparation employed a photo-oxidation-induced crosslinking mechanism. First, furfuryl groups were incorporated into the gelatin. Second, the modified gelatin was mixed in water with Rose Bengal, which is a visible light sensitizer. Irradiation by visible light solidified the aqueous solution. In addition, when the solution was cast on a plate, dried and photo-irradiated in the presence of a photomask a micropattern was formed that matched the micropattern on the photomask. The gelatin-immobilized regions enhanced cell adhesion. It was also confirmed that the gelatin incorporating furfuryl and Rose Bengal have no significant toxicity. The photo-crosslinkable gelatin was employed as a direct pulp capping material in the dental field. Considering these results, this system could be useful as a new type of visible light-induced crosslinkable biosealant.
authors.affiliation: RIKEN Advanced Science Institute, Wako-shi, Saitama, Japan.
authors.forename: Tae Il
authors.initials: TI
authors.lastname: Son
authors.forename: Makoto
authors.initials: M
authors.lastname: Sakuragi
authors.forename: Sawa
authors.initials: S
authors.lastname: Takahashi
authors.forename: Sei
authors.initials: S
authors.lastname: Obuse
authors.forename: Jeonghwa
authors.initials: J
authors.lastname: Kang
authors.forename: Masako
authors.initials: M
authors.lastname: Fujishiro
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Matsushita
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Gong
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tajima
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Toshio
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry / Q000528:radiation effects; D008027:Light; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D010777:Photochemistry / Q000379:methods*; D057872:Pulp Capping and Pulpectomy Agents / Q000737:chemistry / Q000528:radiation effects; D051381:Rats; D012395:Rose Bengal / Q000737:chemistry; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D057872:Pulp Capping and Pulpectomy Agents; D012395:Rose Bengal; D005780:Gelatin