Lower SCD expression in dendritic cells compared to macrophages leads to membrane lipids with less mono-unsaturated fatty acids.
Macrophages and dendritic cells originate from a common myeloid precursor. Although several studies compared transcriptional profiles of these cells, not a single study compared their lipid profiles. Therefore, we measured and compared fatty acid (FA) and phospholipid (PL) species composition of granulocyte/macrophage colony-stimulating factor (GM-CSF) plus interleukin 4 (IL-4) and macrophage colony-stimulating factor (M-CSF) differentiated monocytes isolated from healthy volunteers. We found that these two cell types prominently differ in their FA composition. Dendritic cells (DCs) contain lower proportions of C16 and C18 mono-unsaturated FAs, but higher proportions of C20 and C22 poly-unsaturated fatty acids (PUFAs) than phagocytic macrophages. Analysis of PL species profiles revealed that GM-CSF/IL-4 differentiated cells have increased amounts of longer and more desaturated phospatidylethanolamine (PE) and phosphatidylserine (PS) species, but lower amounts of shorter and less desaturated PE and PS species than M-CSF differentiated cells. These cell type specific lipid profiles can be attributed to a differential expression and activity of stearoyl-CoA desaturase (SCD). Taken together, our results show that GM-CSF/IL-4 compared to M-CSF differentiated monocytes have less mono-unsaturated FA and PL species, which are due to lower SCD activity observed in DCs.
Ecker
Josef
J
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg Germany.
Liebisch
Gerhard
G
Grandl
Margot
M
Schmitz
Gerd
G
D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D009000:Monocytes / Q000166:cytology; D010586:Phagocytes / Q000378:metabolism* / Q000473:pathology; D010743:Phospholipids / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
D005229:Fatty Acids, Monounsaturated; D010743:Phospholipids; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013230:Stearoyl-CoA Desaturase