Antibiotic resistance of Gram negative bacilli strains isolated from the Intensive Care Unit in Fundeni Clinical Institute, Bucharest, Romania.
The aim of this study was to investigate the antibiotic resistance profile of 58 Gram negative bacilli strains (GNB): 36 non-fermentative GNB (NGNB), including 19 strains of Acinetobacter spp., 11 of Pseudomonas aeruginosa, 6 of Stenotrophomonas maltophilia and 22 enterobacterial strains (14 strains of KEHSs, 6 belonging to the group Proteus-Providencia and 2 Escherichia coli) isolated from nasal, pharyngeal exudates and also from bronchial secretions, from immuno-depressed patients admitted in the Intensive Care Unit of Fundeni Clinical Institute. METHHODS: the antibiotic susceptibility testing was performed according to CLSI 2009 recommendations and the production of beta-lactamases was investigated by ESBL chromogenic media, double disc diffusion test, ESBL E-test, Amp C E-test and MBL E-test.


RESULTS
68% of the enterobacterial strains produced extended-spectrum beta- lactamases (ESBL), 13.63% of them expressing simultaneously the Amp C enzyme. All enterobacterial strains were susceptible to carbapenems (Imipenem and Ertapenem). Metallo-beta lactamases production among NGNB strains with resistance to Imipenem was high (80%), these strains being also multi-resistant to the majority of tested antibiotics with the exception of colistin.


CONCLUSIONS
our results showed that the majority of the analyzed strains were multi-drug resistant. Antibiotic multi-resistance and the increasing number of severe infections caused by these strains are a major issue for ICU, where patients with severe diseases and destabilized physiological condition are often admitted.
Borcan
Elvira
E
Bacteriology Laboratory of Fundeni Clinical Institute, Sos. Fundeni 258, Bucharest, Romania.
Ghiţă
Camelia Mihaela
CM
Chifiriuc
Mariana Carmen
MC
Măruţescu
Luminiţa
L
Isar
Cătălina
C
Lazăr
Veronica
V
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D008826:Microbial Sensitivity Tests / Q000379:methods; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D012383:Romania / Q000453:epidemiology; D001618:beta-Lactamases / Q000145:classification / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases