Activities of enzymes that hydrolyze adenine nucleotides in platelets from patients with rheumatoid arthritis.
OBJECTIVE
To investigate whether there are changes in the activity of the enzymes NTPDase, 5'-nucleotidase, E-NPP and ADA in platelets from patients with rheumatoid arthritis (RA).


METHODS
Thirty-five RA patients diagnosed with RA through American College of Rheumatology criteria, as well as 35 healthy patients were selected. NTPDase, 5'-nucleotidase, E-NPP and ADA activities were verified in platelets isolated from these patients.


RESULTS
The results demonstrate that an increase in NTPDase (approximately 100%), 5'-nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) activities occurred in RA patients when compared to the control group.


CONCLUSIONS
Ours results suggest an increase in the NTPDase, 5'-nucleotidase and E-NPP activities, which could be related to a compensatory organic response to excessive platelet aggregation which occurs during the inflammation. The increased ADA activity found in this work could lead to a decrease in the adenosine concentration in the circulation, which could explain the accelerated atherosclerosis found in patients with RA.
Becker
Lara V
LV
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av Roraima, 97105-900 Santa Maria, RS, Brazil.
Rosa
Cintia S
CS
Souza
Viviane do C G
Vdo C
Bagatini
Margarete D
MD
Casali
Emerson A
EA
Leal
Claudio Alberto M
CA
da Silva
João Carlos N
JC
Moretto
Maria Beatriz
MB
Pinheiro
Francielle de V
Fde V
Morsch
Vera Maria
VM
Schetinger
Maria Rosa C
MR
Leal
Daniela B R
DB
D015720:5'-Nucleotidase; D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D050197:Atherosclerosis / Q000209:etiology; D001792:Blood Platelets / Q000201:enzymology* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases
D000227:Adenine Nucleotides; D006867:Hydrolases; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; D000243:Adenosine Deaminase; D011755:Pyrophosphatases