[Study on preparation of icaritin by enzymolysis of icariin with snail hydrolase].
OBJECTIVE
To optimize the conditions for enzymolysis of Icariin by snail hydrolase.


METHODS
Take conversion rate as index, the effects of pH, temperature, reaction time, dosage of enzyme, concentration of Icariin and metal ion on hydrolysis were studied by single-factor designs and a L9 (3(4)) orthogonal design. The product was characterized through MS, 1H-NMR and 13C-NMR methods.


RESULTS
The optimum enzymolysis conversion rate was achieved at 37 degrees C, pH 6.0 acetic acid-sodium acetate buffer solution and 48 h. The Ca2+, Mg2+ and Zn2+ had activation on helicase slightly but the Fe2+ inhibited enzymolysis significantly. The production was presumed as icaritin on the basis of spectral evidences.


CONCLUSIONS
Snail hydrolase can be used for preparing of icaritin. The condition is mildness and suitable for industrialization.
Jia
Dongsheng
D
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Jia
Xiaobin
X
Xue
Jing
J
Shi
Feng
F
Sun
E
E
Chen
Lingling
L
Zhang
Zhenhai
Z
D000818:Animals; D005419:Flavonoids / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D012908:Snails / Q000201:enzymology*; D013696:Temperature
D005419:Flavonoids; D008670:Metals; D006867:Hydrolases; C499403:icaritin