title: Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.
abstract: We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
authors.affiliation: Centre of Molecular Biology and Gene Therapy, Department of Internal Medicine-Hematooncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic. khrncirova@fnbrno.cz
authors.forename: Kristyna
authors.initials: K
authors.lastname: Hrncirova
authors.forename: Martina
authors.initials: M
authors.lastname: Lengerova
authors.forename: Iva
authors.initials: I
authors.lastname: Kocmanova
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Racil
authors.forename: Pavlina
authors.initials: P
authors.lastname: Volfova
authors.forename: Dita
authors.initials: D
authors.lastname: Palousova
authors.forename: Mojmir
authors.initials: M
authors.lastname: Moulis
authors.forename: Barbora
authors.initials: B
authors.lastname: Weinbergerova
authors.forename: Jana
authors.initials: J
authors.lastname: Winterova
authors.forename: Martina
authors.initials: M
authors.lastname: Toskova
authors.forename: Sarka
authors.initials: S
authors.lastname: Pospisilova
authors.forename: Jiri
authors.initials: J
authors.lastname: Mayer
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009090:Mucorales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009091:Mucormycosis / Q000175:diagnosis*; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D044366:Transition Temperature
chemical_list: D004271:DNA, Fungal