title: Expression of cathepsins in human skin photoaging.
abstract: Cathepsins are involved in regulatory mechanisms in human skin, but their role in photoaged skin remains unknown. This study investigates the role of cathepsin B, D, K, and G in skin photoaging in vivo and in vitro. Cathepsin-induced changes in skin as a result of chronic UV irradiation were detected by immunohistochemistry methods. Protein cathepsin expressions in UVA-induced premature senescence in fibroblasts in vitro were detected by Western blot technique. Cathepsin mRNA expression in photoaged skin and fibroblasts was detected by real-time reverse transcription-polymerase chain reaction. Immunohistochemistry and Western blot show lower protein expression of cathepsin B, D, and K in photoaged skin and fibroblasts, while cathepsin G was higher. The mRNA expression of cathepsin B, D, and K of the photoaged skin in vivo decreased to 20 ± 0.5, 25 ± 1.6 and 22 ± 0.8%, while cathepsin G mRNA increased to 2.24 ± 0.09 times that of control. In photoaged fibroblasts, cathepsin B, D, and K mRNA was downregulated to 64 ± 2.9, 24 ± 2.1 and 9 ± 0.5% while cathepsin G mRNA was upregulated to 1.42 ± 0.06 times that of control fibroblasts. These experiments suggest that cathepsin B, D, K, and G may act as biomarkers in photoaged human skin.
authors.affiliation: Department of Dermato-Venereology, The Third Affiliated Hospital of Sun Yat-sen University, #600 Tianhe Road, Guangzhou, Guangdong, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.forename: W
authors.initials: W
authors.lastname: Lai
authors.forename: M
authors.initials: M
authors.lastname: Wan
authors.forename: H I
authors.initials: HI
authors.lastname: Maibach
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002401:Cathepsin B / Q000235:genetics / Q000378:metabolism; D002402:Cathepsin D / Q000235:genetics / Q000378:metabolism; D056649:Cathepsin G / Q000235:genetics / Q000378:metabolism; D056657:Cathepsin K / Q000235:genetics / Q000378:metabolism; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D015595:Skin Aging; D014466:Ultraviolet Rays
chemical_list: D012333:RNA, Messenger; D002403:Cathepsins; D056649:Cathepsin G; D002401:Cathepsin B; D056657:Cathepsin K; D002402:Cathepsin D