Identification of Galectin-1 as a novel biomarker in nasopharyngeal carcinoma by proteomic analysis.
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in southern China and Southeast Asia. It is characterized as a multistep process involved in multiple genetic and epigenetic events. The mechanism of carcinogenesis still needs to be further clarified. In this study, two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), RT-PCR, Western blot and immunohistochemical (IHC) analyses were used to detect Galectin-1 expression in NPC compared with normal nasopharyngeal epithelial tissues (NNET). We found that Galectin-1 was expressed at a significantly higher level in NPC compared with NNET. Our results indicated that high expression level of Galectin-1 might correlate with the development of NPC and Galectin-1 may serve as a potential diagnostic marker or therapeutic target for NPC.
Tang
Can-E
CE
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Medicine Research Center, Xiangya Hospital, Central South University, Changsha 410008, PR China.
Tan
Tan
T
Li
Cui
C
Chen
Zhu-Chu
ZC
Ruan
Lin
L
Wang
Hong-Han
HH
Su
Tao
T
Zhang
Peng-Fei
PF
Xiao
Zhi-Qiang
ZQ
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D037483:Galectin 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D037483:Galectin 1