Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles.
In this study, a method for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC-FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid-0.2 m MgCl(2) in water and acetonitrile as an extraction solvent, and n-hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents.
Choi
Jeong-Heui
JH
Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, Republic of Korea.
Na
Tae Woong
TW
Mamun
Md Iqbal Rouf
MI
Abd El-Aty
A M
AM
Shin
Eun-Ho
EH
Park
Jong-Hyouk
JH
Shim
Jae-Han
JH
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002021:Buffers; D005591:Chemical Fractionation / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005470:Fluorometry / Q000379:methods; D005504:Food Analysis / Q000379:methods; D006586:Hexanes / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
D000097:Acetonitriles; D002021:Buffers; D006586:Hexanes; C026385:n-hexane; D002939:Ciprofloxacin; C047224:sarafloxacin; C032159:acetonitrile