Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction.
Isolation of adipose-derived stem cells (ASCs) typically involves 8+ hours of intense effort, requiring specialized equipment and reagents. Here, we present an improved technique for isolating viable populations of mesenchymal stem cells from lipoaspirate saline fractions within 30 minutes. Importantly, the cells exhibit remarkable similarities to those obtained using the traditional isolation protocols, in terms of their multipotent differentiation potential and immunophenotype. Reducing the acquisition time of ASCs is critical for advancing regenerative medicine therapeutics, and our approach provides rapid and simple techniques for enhanced isolation and expansion of patient-derived mesenchymal stem cells.
Francis
Michael P
MP
Department of Pathology, Medical College of Virginia Campus at Virginia Commonwealth University, Richmond, VA, USA.
Sachs
Patrick C
PC
Elmore
Lynne W
LW
Holt
Shawn E
SE
D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D020219:Chondrogenesis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis