High variability and rapid evolution of a nanovirus.
Nanoviruses are multipartite single-stranded DNA (ssDNA) plant viruses that cause important diseases of leguminous crops and banana. Little has been known about the variability and molecular evolution of these viruses. Here we report on the variability of faba bean necrotic stunt virus (FBNSV), a nanovirus from Ethiopia. We found mutation frequencies of 7.52 x 10(-4) substitutions per nucleotide in a field population of the virus and 5.07 x 10(-4) substitutions per nucleotide in a laboratory-maintained population derived thereof. Based on virus propagation for a period of more than 2 years, we determined a nucleotide substitution rate of 1.78 x 10(-3) substitutions per nucleotide per year. This high molecular evolution rate places FBNSV, as a representative of the family Nanoviridae, among the fastest-evolving ssDNA viruses infecting plants or vertebrates.
Grigoras
Ioana
I
Institut des Sciences du Végétal, CNRS, Avenue de la Terrasse, Bât. 23, 91198 Gif sur Yvette, France.
Timchenko
Tatiana
T
Grande-Pérez
Ana
A
Katul
Lina
L
Vetten
Heinrich-Josef
HJ
Gronenborn
Bruno
B
D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005002:Ethiopia; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D025682:Nanovirus / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000821:virology; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D031307:Vicia faba / Q000821:virology
D004279:DNA, Viral