Fusarium verticillioides: evaluation of fumonisin production and effect of fungicides on in vitro inhibition of mycelial growth.
The objectives of this study were to evaluate the fumonisin production by 16 F. verticillioides strains on corn cultures and the effect of quintozene and fludioxonil + metalaxyl-M fungicides on "in vitro" mycelial growth on agar. In addition, the effect of fludioxonil + metalaxyl-M on fumonisin production in defined liquid culture medium was analyzed. Fumonisin B(1) levels on corn cultures ranged from 2.41 to 3996.36 μg/g and the F. verticillioides 103F strain produced the highest level (3996.36 ± 390.49 μg/g, P < 0.05). F. verticillioides strains were inoculated in potato dextrose agar with the addition of quintozene (75 to 9,375 μg/ml) and fludioxonil + metalaxyl-M (1.5 + 0.6 to 187.5 + 75 μg/ml) in order to evaluate the effect of these fungicides on "in vitro" mycelial growth. The F. verticillioides strains showed great variability concerning ED(50) values, which were below the recommended application dose for quintozene, but above that for fludioxonil + metalaxyl-M. Moreover, fungicide addition to the culture medium increased mean FB(1) levels compared to the control, suggesting the importance of focusing on the effect of fungicides on mycotoxin production as well as on the phytopathogen control.
Falcão
Virgínia Carla A
VC
Department of Biochemistry and Biotechnology, Center of Exact Sciences, State University of Londrina, P.O. Box 6001, Londrina, Paraná, 86051-980, Brazil.
Ono
Mario Augusto
MA
de Ávila Miguel
Tatiana
T
Vizoni
Edio
E
Hirooka
Elisa Yoko
EY
Ono
Elisabete Yurie Sataque
EY
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D004149:Dioxoles / Q000494:pharmacology; D037341:Fumonisins / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D025282:Mycelium / Q000187:drug effects* / Q000254:growth & development*; D009578:Nitrobenzenes / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D003313:Zea mays / Q000382:microbiology
D003470:Culture Media; D004149:Dioxoles; D037341:Fumonisins; D005659:Fungicides, Industrial; D009578:Nitrobenzenes; D011758:Pyrroles; C028175:metalaxyl; C108339:fludioxonil; D000409:Alanine; C005805:quintozene