Toxic effects and specific chromium acquired resistance in selected strains of Dyctiosphaerium chlorelloides.
Due to its various uses, chromium contamination has become widespread in a diverse array of environments. The present study was carried out to investigate the toxic effect of chromium exposures on sensitive and resistant strains of the green algae Dyctiosphaerium chlorelloides, and to determine the nature and mechanism of chromium-resistant cells that arise. The toxic effect on the photosynthetic performance of chromium exposures in both cell populations, and the sensitive differences due to chromium oxidation state, were estimated, and the results indicate that although the photosynthetic performance in both strains were inhibited, there are not significant differences among IC(50(72)) values obtained in toxicity assays with both chromium oxidation states in wild-type cells, and however these differences are very significant when the assays were performed with Cr(VI) resistant cells. The 72-h 50% inhibitory concentration values obtained with Cr(III) exposures were similar for both strains. Additionally, by means of the SEM/EDX and TEM microscopic techniques, the occurrence of rapid morphological evolution in the microalgal cells and the possible detoxificant mechanisms was observed after exposure of the wild strain to chromium hexavalent. Moreover, the different response in photosynthetic activity observed between sensitive and resistant cells of D. chlorelloides in the presence of Cr(VI) and Cr(III) could be used to obtain a chromium-specific eukaryotic microalgal biosensor.
D'ors
A
A
Department of Toxicology and Pharmacology, School of Veterinary Sciences, Complutense University, s/n Puerta de Hierro Ave., 28040 Madrid, Spain.
Pereira
M
M
Bartolomé
M C
MC
López-Rodas
V
V
Costas
E
E
Sánchez-Fortún
S
S
D000460:Chlorophyta / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D002857:Chromium / Q000633:toxicity*; D004351:Drug Resistance; D008855:Microscopy, Electron, Scanning; D010788:Photosynthesis / Q000187:drug effects; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D002857:Chromium