Simultaneous production of lipases and biosurfactants by submerged and solid-state bioprocesses.
Lipases and biosurfactants are compounds produced by microorganisms generally involved in the metabolization of oil substrates. However, the relationship between the production of lipases and biosurfactants has not been established yet. Therefore, this study aimed to evaluate the correlation between production of lipases and biosurfactants by submerged (SmgB) and solid-state bioprocess (SSB) using Aspergillus spp., which were isolated from a soil contaminated by diesel oil. SSB had the highest production of lipases, with lipolytic activities of 25.22U, while SmgB had 4.52U. The production of biosurfactants was not observed in the SSB. In the SmgB, correlation coefficients of 91% and 87% were obtained between lipolytic activity and oil in water and water in oil emulsifying activities, respectively. A correlation of 84% was obtained between lipolytic activity and reduction of surface tension in the culture medium. The surface tension decreased from 50 to 28mNm(-1) indicating that biosurfactants were produced in the culture medium.
Colla
Luciane Maria
LM
Laboratory of Fermentations, Course of Food Engineering, College of Engineering and Architecture, University of Passo Fundo, Campus I, km 171, BR 285, P.O. Box 611, CEP 99001-970, Passo Fundo, RS, Brazil.
Rizzardi
Juliana
J
Pinto
Marta Heidtmann
MH
Reinehr
Christian Oliveira
CO
Bertolin
Telma Elita
TE
Costa
Jorge Alberto Vieira
JA
D001230:Aspergillus / Q000201:enzymology / Q000254:growth & development; D001709:Biotechnology / Q000379:methods*; D004655:Emulsions; D008049:Lipase / Q000096:biosynthesis*; D008066:Lipolysis; D009821:Oils / Q000378:metabolism; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*; D013997:Time Factors; D014867:Water / Q000378:metabolism
D004655:Emulsions; D009821:Oils; D013501:Surface-Active Agents; D014867:Water; D008049:Lipase