Differential effects of radiation and age on diffusion tensor imaging in rats.
Greater than 50% of adults and approximately 100% of children who survive >6 months after fractionated partial or whole-brain radiotherapy develop cognitive impairments. Noninvasive methods are needed for detecting and tracking the radiation-induced brain injury associated with these impairments. Using magnetic resonance imaging, we sought to detect structural changes associated with brain injury in our rodent model of fractionated whole-brain irradiation (fWBI) induced cognitive impairment and to compare those changes with alterations that occur during the aging process. Middle aged rats were given a clinically relevant dose of fWBI (40 Gy: two 5 Gy fractions/week for 4 weeks) and scanned approximately 1 year post-irradiation to obtain whole-brain T2 and diffusion tensor images (DTI); control groups of sham-irradiated age-matched and young rats were also scanned. No gross structural changes were evident in the T2 structural images, and no detectable fWBI-induced DTI changes in fractional anisotropy (FA) were found in heavily myelinated white matter (corpus callosum, cingulum, and deep cortical white matter). However, significant fWBI-induced variability in FA distribution was present in the superficial parietal cortex due to an fWBI-induced decline in FA in the more anterior slices through parietal cortex. Young rats had significantly lower FA values relative to both groups of older rats, but only within the corpus callosum. These findings suggest that targets of the fWBI-induced change in this model may be the less myelinated or unmyelinated axons, extracellular matrix, or synaptic fields rather than heavily myelinated tracts.
Peiffer
Ann M
AM
Department of Radiology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. Electronic address: apeiffer@wfubmc.edu.
Shi
Lei
L
Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Olson
John
J
Center for Biomolecular Imaging, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Brunso-Bechtold
Judy K
JK
Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
D000367:Age Factors; D000375:Aging / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D016880:Anisotropy; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects*; D056324:Diffusion Tensor Imaging / Q000379:methods; D019583:Dose Fractionation, Radiation; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000528:radiation effects; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344