Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells.
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host.
Zhang
Xiaowei
X
Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, CH J2-506, Station 6, 1015 Lausanne, Switzerland.
Garcia
Isabel Fernandez
IF
Baldi
Lucia
L
Hacker
David L
DL
Wurm
Florian M
FM
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry; D009997:Osmotic Pressure; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012462:Saline Solution, Hypertonic / Q000378:metabolism
D003470:Culture Media; D011994:Recombinant Proteins; D012462:Saline Solution, Hypertonic