High-resolution typing by integration of genome sequencing data in a large tuberculosis cluster.
To investigate whether genome sequencing yields more useful markers than those currently used to study the epidemiology of tuberculosis, it was applied to three Mycobacterium tuberculosis isolates of the Harlingen outbreak. Our findings suggest that single nucleotide polymorphisms can be used to identify transmission chains in restriction fragment length polymorphism clusters.
Schürch
Anita C
AC
Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control, P.O. Box 1, 3720 BA Bilthoven, the Netherlands.
Kremer
Kristin
K
Daviena
Olaf
O
Kiers
Albert
A
Boeree
Martin J
MJ
Siezen
Roland J
RJ
van Soolingen
Dick
D
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000382:microbiology*
D004269:DNA, Bacterial