title: Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1).
abstract: The liver fluke, Fasciola hepatica causes liver fluke disease, or fasciolosis, in ruminants such as cattle and sheep. An effective vaccine against the helminth parasite is essential to reduce our reliance on anthelmintics, particularly in light of frequent reports of resistance to some frontline drugs. In our study, Friesian cattle (13 per group) were vaccinated with recombinant F. hepatica cathepsin L1 protease (rFhCL1) formulated in mineral-oil based adjuvants, Montanide ISA 70VG and ISA 206VG. Following vaccination the animals were exposed to fluke-contaminated pastures for 13 weeks. At slaughter, there was a significant reduction in fluke burden of 48.2% in the cattle in both vaccinated groups, relative to the control non-vaccinated group, at p<or=0.05. All vaccinated animals showed a sharp rise in total IgG levels to rFhCL1 post-vaccination which was maintained over the course of the 13-week challenge infection and was significantly higher than levels reached in the control group. Arginase levels in the macrophages of vaccinated cattle were significantly lower than those of the control cattle, indicating that the parasite-induced alternative-activation of the macrophages was altered by vaccination. The data demonstrate the potential for recombinant FhCL1 vaccine in controlling fasciolosis in cattle under field conditions.
authors.affiliation: School of Agriculture, Food Science and Veterinary Medicine, UCD, Belfield, Dublin 4, Ireland. olwen.golden@ucd.ie
authors.forename: O
authors.initials: O
authors.lastname: Golden
authors.forename: R J
authors.initials: RJ
authors.lastname: Flynn
authors.forename: C
authors.initials: C
authors.lastname: Read
authors.forename: M
authors.initials: M
authors.lastname: Sekiya
authors.forename: S M
authors.initials: SM
authors.lastname: Donnelly
authors.forename: C
authors.initials: C
authors.lastname: Stack
authors.forename: J P
authors.initials: JP
authors.lastname: Dalton
authors.forename: G
authors.initials: G
authors.lastname: Mulcahy
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000915:Antibody Affinity; D000947:Antigens, Helminth / Q000276:immunology*; D001119:Arginase / Q000378:metabolism; D002403:Cathepsins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D008264:Macrophages / Q000276:immunology; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D002403:Cathepsins; C509464:cathepsin L1, Fasciola hepatica; D001119:Arginase