title: Laser assisted bioprinting of engineered tissue with high cell density and microscale organization.
abstract: Over this decade, cell printing strategy has emerged as one of the promising approaches to organize cells in two and three dimensional engineered tissues. High resolution and high speed organization of cells are some of the key requirements for the successful fabrication of cell-containing two or three dimensional constructs. So far, none of the available cell printing technologies has shown an ability to concomitantly print cells at a cell-level resolution and at a kHz range speed. We have studied the effect of the viscosity of the bioink, laser energy, and laser printing speed on the resolution of cell printing. Accordingly, we demonstrate that a laser assisted cell printer can deposit cells with a microscale resolution, at a speed of 5 kHz and with computer assisted geometric control. We have successfully implemented such a cell printing precision to print miniaturized tissue like layouts with de novo high cell density and micro scale organization.
authors.affiliation: Université Victor Segalen Bordeaux 2, Bordeaux cedex, France. bertrand.guillotin@inserm.fr
authors.forename: Bertrand
authors.initials: B
authors.lastname: Guillotin
authors.forename: Agnès
authors.initials: A
authors.lastname: Souquet
authors.forename: Sylvain
authors.initials: S
authors.lastname: Catros
authors.forename: Martí
authors.initials: M
authors.lastname: Duocastella
authors.forename: Benjamin
authors.initials: B
authors.lastname: Pippenger
authors.forename: Séverine
authors.initials: S
authors.lastname: Bellance
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Murielle
authors.initials: M
authors.lastname: Rémy
authors.forename: Laurence
authors.initials: L
authors.lastname: Bordenave
authors.forename: Joëlle
authors.initials: J
authors.lastname: Amédée
authors.forename: Fabien
authors.initials: F
authors.lastname: Guillemot
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002460:Cell Line; D016287:Computer Peripherals; D006801:Humans; D007834:Lasers; D008422:Materials Testing; D011327:Printing / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014783:Viscosity