title: Immobilization strategies for laccase from Trametes versicolor on mesostructured silica materials and the application to the degradation of naphthalene.
abstract: The oxidation of naphthalene by immobilized laccase from Trametes versicolor has been performed using diverse immobilization strategies on mesostructured silica materials. Laccase was immobilized by physical adsorption on several SBA-15 with different textural properties and by covalent attachment on functionalized SBA-15 prepared by co-condensation method (direct synthesis). The adsorption of laccase was partially reversible and showed some degree of lixiviation. However, covalently attached laccase to aminopropyl and aminobutyl functionalized SBA-15 exhibited important activity for the degradation of naphthalene with, respectively, 35% and 39%wt of removal in 5h. The aminopropyl biocatalyst retained higher activity after repeated uses than the corresponding aminobutyl.
authors.affiliation: Department of Chemical and Environmental Technology, ESCET, Universidad Rey Juan Carlos. C/ Tulipán s/n, Móstoles, Madrid, Spain. fernando.bautista@urjc.es
authors.forename: L
authors.initials: L
authors.lastname: Fernando Bautista
authors.forename: Gabriel
authors.initials: G
authors.lastname: Morales
authors.forename: Raquel
authors.initials: R
authors.lastname: Sanz
mesh_terms: D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D008567:Membranes, Artificial; D009281:Naphthalenes / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D055454:Trametes / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009281:Naphthalenes; C031721:naphthalene; D012822:Silicon Dioxide; D042845:Laccase