Effects of S100A9 in a rat model of asthma and in isolated tracheal spirals.
S100A9 is a member of the S100 family of proteins that contain two EF-hand calcium-binding motifs. We previously reported that S100A9 was differentially expressed during the early airway response phase of asthma and can be regulated by acupuncture. To understand the possible role of S100A9 in asthma, the effects of the S100A9 were investigated in a rat model of asthma and in isolated tracheal spirals. The pulmonary function and isometric tension were measured after the administration of purified recombinant S100A9. The results of in vivo experiments showed that S100A9 (0.1microg/kg) significantly decreased the pulmonary resistance and increased the dynamic compliance. The in vitro experimental results showed that S100A9 (100, 200, 400, or 800ng/ml, final concentrations) significantly reduced the isometric tension of isolated tracheal spirals. These results suggest that S100A9 elicits dose-dependent anti-asthmatic effects and may provide further insight into the treatment of asthma.
Yin
Lei-Miao
LM
Laboratory of Molecular Biology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Li
Hai-Yan
HY
Zhang
Qing-Hua
QH
Xu
Yu-Dong
YD
Wang
Yu
Y
Jiang
Yong-Liang
YL
Wei
Ying
Y
Liu
Yan-Yan
YY
Yang
Yong-Qing
YQ
D000403:Airway Resistance / Q000187:drug effects*; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D001249:Asthma / Q000503:physiopathology / Q000628:therapy*; D040502:Calgranulin B / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D014132:Trachea / Q000187:drug effects
D018927:Anti-Asthmatic Agents; D040502:Calgranulin B; D011993:Recombinant Fusion Proteins