title: Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction.
abstract: Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric oxide synthase levels [1,2]. Accordingly, the goal of this study was to determine how the LV infarct proteome is altered by a post-MI exercise regimen. Sprague-Dawley rats underwent ligation of the left descending coronary artery to induce MI. Exercise training was initiated four weeks post-MI and continued for 8 weeks in n=12 rats. Compared with the sedentary MI group (n=10), the infarct region of rats receiving exercise showed 20 protein spots with altered intensities in two-dimensional gels (15 increased and 5 decreased; p<0.05). Of 52 proteins identified in 20 spots, decreased levels of voltage-dependent anion-selective channel 2 and increased levels of glutathione perioxidase and manganese superoxide were confirmed by immunoblotting. Cardiac function was preserved in rats receiving exercise training, and the beneficial effect was linked with changes in these 3 proteins. In conclusion, our results suggest that post-MI exercise training increases anti-oxidant levels and decreases ion channel levels, which may explain, in part, the improved cardiac function seen with exercise.
authors.affiliation: Department of Medicine, Division of Cardiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
authors.forename: Arvin
authors.initials: A
authors.lastname: Bansal
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Dai
authors.forename: Ying Ann
authors.initials: YA
authors.lastname: Chiao
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Hakala
authors.forename: John Q
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Susan T
authors.initials: ST
authors.lastname: Weintraub
authors.forename: Merry L
authors.initials: ML
authors.lastname: Lindsey
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000503:physiopathology; D013058:Mass Spectrometry; D009203:Myocardial Infarction / Q000503:physiopathology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects; D050996:Voltage-Dependent Anion Channel 2 / Q000378:metabolism
chemical_list: D000975:Antioxidants; C494767:Vdac2 protein, rat; D050996:Voltage-Dependent Anion Channel 2; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase