Zeta potential based colorimetric immunoassay for the direct detection of diabetic marker HbA1c using gold nanoprobes.
A one-step homogeneous colorimetric immunoassay format coupled with zeta potential measurements for determination of specific diabetic biomarker glycated hemoglobin (HbA1c) using functionalised gold nanoparticles as bioprobes is reported. The assay exhibited an excellent sensitivity based on absorbance and zeta potential measurements showing the dynamic response range from 0.001-0.004 mg mL(-1) for HbA1c with a detection limit of 0.0015 mg mL(-1).
Wangoo
Nishima
N
Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160014, India.
Kaushal
Jyotsna
J
Bhasin
K K
KK
Mehta
S K
SK
Suri
C Raman
CR
D015415:Biomarkers / Q000032:analysis; D003124:Colorimetry; D003920:Diabetes Mellitus / Q000175:diagnosis*; D055664:Electrochemical Techniques; D006442:Glycated Hemoglobin / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*
D015415:Biomarkers; D006442:Glycated Hemoglobin; D006046:Gold; C517652:hemoglobin A1c protein, human