Long-term potentiation of neuronal excitation in the central nucleus of the rat amygdala revealed by imaging with a voltage-sensitive dye.
We examined the propagation pattern of neuronal excitation in slices from the central nucleus of the rat amygdala (CeA) using optical imaging with a voltage-sensitive dye. We analyzed the mechanisms for the long-term potentiation (LTP) of neuronal excitation induced by conditioning stimulation. High-frequency conditioning stimulation induced NMDA receptor-, cAMP-, and PKA-dependent LTP of neuronal excitation in the lateral part of the CeA. The bath application of forskolin also induced LTP that was PKA-dependent. These results suggest that the potentiation of neuronal excitation in the lateral part of the CeA is induced by the activation of intracellular cAMP/PKA signaling, which is triggered by the influx of Ca(2+) via NMDA receptors.
Kiritoshi
Takaki
T
Department of Human and Artificial Intelligence Systems, Graduate School of Engineering, and Research and Education Program for Life Science, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan.
Ikeda
Hiroshi
H
Murase
Kazuyuki
K
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000679:Amygdala / Q000166:cytology*; D000818:Animals; D000831:Animals, Newborn; D000841:Anisomycin / Q000494:pharmacology; D001703:Biophysics / Q000379:methods; D002118:Calcium / Q000378:metabolism; D002227:Carbazoles / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D004558:Electric Stimulation / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology; D056969:Voltage-Sensitive Dye Imaging
D002227:Carbazoles; D004791:Enzyme Inhibitors; D018691:Excitatory Amino Acid Antagonists; D011500:Protein Synthesis Inhibitors; D011758:Pyrroles; D005576:Colforsin; C057416:KT 5720; D000841:Anisomycin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine; D002118:Calcium