title: Molecular typing of Bartonella henselae DNA extracted from human clinical specimens and cat isolates in Japan.
abstract: Bartonella henselae is the causative agent of cat scratch disease (CSD). To clarify the population structure and relationship between human and cat strains of B. henselae, 55 specimens isolated in Japan, including 24 B. henselae DNA-positive clinical samples from CSD patients and 31 B. henselae isolates from domestic cats, were characterized by multilocus sequence typing (MLST) and the 16S-23S tRNA-Ala/tRNA-Ile intergenic spacer (S1) sequence, which were used previously for strain typing of B. henselae. Three different sequence types (STs) were identified by MLST, one of which was novel. Fifty-two strains (94.5%), including all strains detected in CSD patients, were assigned to ST-1. Eight S1 genotypes were observed, three of which were novel. The 52 ST-1 strains were classified into seven S1 genotypes, two of which were predominant in both human and cat strains. In addition, 5.5% of the strains (3/55) contained two different intergenic spacer S1 copies. These results indicate that the predominant B. henselae MLST ST-1 in Japan is a significantly genetically diverse population on the basis of the sequence diversity of intergenic spacer S1, and that highly prevalent S1 genotypes among cats are often involved in human infections.
authors.affiliation: Department of Basic Laboratory Sciences, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan. m-yanagi@yamaguchi-u.ac.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Yanagihara
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Tsuneoka
authors.forename: Shoko
authors.initials: S
authors.lastname: Hoshide
authors.forename: Erina
authors.initials: E
authors.lastname: Ishido
authors.forename: Akiko
authors.initials: A
authors.lastname: Umeda
authors.forename: Masato
authors.initials: M
authors.lastname: Tsukahara
authors.forename: Junzo
authors.initials: J
authors.lastname: Nojima
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ichihara
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hino
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D018416:Bartonella henselae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002372:Cat-Scratch Disease / Q000382:microbiology* / Q000662:veterinary*; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D058889:Molecular Typing
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer