Automated quantitative live cell fluorescence microscopy.
Advances in microscopy automation and image analysis have given biologists the tools to attempt large scale systems-level experiments on biological systems using microscope image readout. Fluorescence microscopy has become a standard tool for assaying gene function in RNAi knockdown screens and protein localization studies in eukaryotic systems. Similar high throughput studies can be attempted in prokaryotes, though the difficulties surrounding work at the diffraction limit pose challenges, and targeting essential genes in a high throughput way can be difficult. Here we will discuss efforts to make live-cell fluorescent microscopy based experiments using genetically encoded fluorescent reporters an automated, high throughput, and quantitative endeavor amenable to systems-level experiments in bacteria. We emphasize a quantitative data reduction approach, using simulation to help develop biologically relevant cell measurements that completely characterize the cell image. We give an example of how this type of data can be directly exploited by statistical learning algorithms to discover functional pathways.
Fero
Michael
M
Department of Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA. mike.fero@stanford.edu
Pogliano
Kit
K
D000465:Algorithms; D000818:Animals; D001331:Automation; D002138:Calibration; D002453:Cell Cycle; D001330:Electronic Data Processing; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009154:Mutation; D010641:Phenotype; D034622:RNA Interference
D049452:Green Fluorescent Proteins