A "GC-rich" method for mammalian gene expression: a dominant role of non-coding DNA GC content in regulation of mammalian gene expression.
High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5' or/and 3' flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super "chromatin opening element" and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content.
Jia
Qian
Q
NCPC R&D Center, Shijiazhuang, 050015, China. qianjiazh@hotmail.com.
Wu
HongTao
H
NCPC R&D Center, Shijiazhuang, 050015, China.
Zhou
XingJun
X
NCPC R&D Center, Shijiazhuang, 050015, China.
Gao
Jian
J
NCPC R&D Center, Shijiazhuang, 050015, China.
Zhao
Wei
W
NCPC R&D Center, Shijiazhuang, 050015, China.
Aziz
JouDi
J
AmProtein Inc, Camarillo, CA, 93010, USA.|AmProtein China, Hangzhou, 310019, China.
Wei
JingShuang
J
NCPC R&D Center, Shijiazhuang, 050015, China.
Hou
Lihua
L
Institute of Microbioloy and Epidemiology Academy of Military Medical Sciences, Beijing, 100071, China.
Wu
Shuyin
S
AmProtein China, Hangzhou, 310019, China.
Zhang
Ying
Y
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Dong
XiangFeng
X
NCPC R&D Center, Shijiazhuang, 050015, China.
Huang
YanMin
Y
NCPC R&D Center, Shijiazhuang, 050015, China.
Jin
WeiYuan
W
AmProtein China, Hangzhou, 310019, China.
Zhu
HongJie
H
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Zhao
XinHui
X
Institute of Microbioloy and Epidemiology Academy of Military Medical Sciences, Beijing, 100071, China.
Huang
ChunHua
C
Shanghai Huayi Biotech, Shanghai, 200233, China.
Xing
LiPing
L
AmProtein China, Hangzhou, 310019, China.
Li
Liwen
L
AmProtein China, Hangzhou, 310019, China.
Ma
Jun
J
AmProtein China, Hangzhou, 310019, China.
Liu
Xiyan
X
AmProtein China, Hangzhou, 310019, China.
Tao
Ran
R
AmProtein China, Hangzhou, 310019, China.
Ye
ShuaiDong
S
AmProtein China, Hangzhou, 310019, China.
Song
YiGao
Y
AmProtein China, Hangzhou, 310019, China.
Song
LingLing
L
AmProtein China, Hangzhou, 310019, China.
Chen
GuanPing
G
AmProtein China, Hangzhou, 310019, China.
Du
ChunLing
C
AmProtein China, Hangzhou, 310019, China.
Zhang
XueTing
X
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Li
Bo
B
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Wang
YanTao
Y
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Yang
Wei
W
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Rishton
Gilbert
G
AmProtein Inc, Camarillo, CA, 93010, USA.
Teng
YuYang
Y
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Leng
GouQing
G
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Li
LuanFeng
L
AmProtein China, Hangzhou, 310019, China.
Liu
WenXian
W
NCPC R&D Center, Shijiazhuang, 050015, China.
Cheng
LiJun
L
NCPC R&D Center, Shijiazhuang, 050015, China.
Liang
QiuBo
Q
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Li
ZhengWu
Z
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Zhang
XiuQin
X
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Zuo
Yajun
Y
Shanghai Huayi Biotech, Shanghai, 200233, China.
Chen
Wei
W
Institute of Microbioloy and Epidemiology Academy of Military Medical Sciences, Beijing, 100071, China. chenwei0226@yahoo.com.cn.
Li
Huicheng
H
Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China.
Hui
Matthew Mizhou
MM
NCPC R&D Center, Shijiazhuang, 050015, China. mhui@amprotein.com.|AmProtein Inc, Camarillo, CA, 93010, USA. mhui@amprotein.com.|Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China. mhui@amprotein.com.|AmProtein China, Hangzhou, 310019, China. mhui@amprotein.com.
D000199:Actins / Q000235:genetics; D000818:Animals; D001482:Base Composition / Q000235:genetics*; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D002645:Chickens; D002843:Chromatin / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D007438:Introns / Q000235:genetics*; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D014162:Transfection
D000199:Actins; D002843:Chromatin; D004247:DNA