title: Repository of Eurasian influenza A virus hemagglutinin and neuraminidase reverse genetics vectors and recombinant viruses.
abstract: Reverse genetics can be used to produce recombinant influenza A viruses containing virtually every desired combination of hemagglutinin (HA) and neuraminidase (NA) genes using the virus backbone of choice. Here, a repository of plasmids and recombinant viruses representing all contemporary Eurasian HA and NA subtypes, H1-H16 and N1-N9, was established. HA and NA genes were selected based on sequence analyses of influenza virus genes available from public databases. Prototype Eurasian HA and NA genes were cloned in bidirectional reverse genetics plasmids. Recombinant viruses based on the virus backbone of A/PR/8/34, and containing a variety of HA and NA genes were produced in 293T cells. Virus stocks were produced in MDCK cells and embryonated chicken eggs. These plasmids and viruses may be useful for numerous purposes, including influenza virus research projects, vaccination studies, and to serve as reference reagents in diagnostic settings.
authors.affiliation: National Influenza Centre and Department of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Keawcharoen
authors.forename: M I J
authors.initials: MI
authors.lastname: Spronken
authors.forename: O
authors.initials: O
authors.lastname: Vuong
authors.forename: T M
authors.initials: TM
authors.lastname: Bestebroer
authors.forename: V J
authors.initials: VJ
authors.lastname: Munster
authors.forename: A D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: G F
authors.initials: GF
authors.lastname: Rimmelzwaan
authors.forename: R A M
authors.initials: RA
authors.lastname: Fouchier
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D004285:Dogs; D005814:Genes, Viral; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D010063:Ovum / Q000821:virology; D010802:Phylogeny; D010957:Plasmids; D011995:Recombination, Genetic; D014776:Virus Cultivation
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase