The cocaine- and amphetamine-regulated transcript (CART) immunoreactivity in the amygdala of the pig.
The distribution and morphology of neurons containing cocaine- and amphetamine-regulated transcript (CART) was investigated in the pig amygdala. CART- immunoreactive (CART-IR) cell bodies were rarely observed in the pig amygdala and most often they were present in the posterior (small-celled) parts of the basolateral and basomedial nuclei. In all other subdivisions only a small number of randomly scattered pericarya were present. In every region studied the CART-IR neurons formed a heterogeneous population consisting mostly of small, rounded or slightly elongated cell bodies, with a few poorly branched, smooth dendrites. In general, the morphological features of these cells clearly resembled non-pyramidal Golgi type II interneurons. Some randomly scattered CART-IR cell bodies were significantly larger and they demonstrated features of pyramidal-like Golgi type I projecting neurons. The highest densities of CART-IR fibres were evident within the central and medial nuclei. Moderate to high expression was found within the large-celled part of the basolateral nucleus and moderate to low levels in the lateral, basomedial and cortical nuclei. The routine double-labelling studies with antisera directed against CART and somatostatin (SOM), or neuropeptide Y (NPY), or cholecystokinin (CCK), or vasoactive intestinal peptide (VIP), or substance P (SP) demonstrated that, in general, these peptides do not co-exist in the CART-IR neurons. However, small subpopulations of the CART-IR fibres contained SOM, CCK, VIP or SP together.
Równiak
M
M
Department of Comparative Anatomy, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Poland. mrowniak@moskit.uwm.edu.pl
Robak
A
A
Bogus-Nowakowska
K
K
Wasilewska
B
B
Najdzion
J
J
Majewski
M
M
D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002766:Cholecystokinin / Q000032:analysis; D003712:Dendrites / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009478:Neuropeptide Y / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013552:Swine / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D002766:Cholecystokinin