title: Organogenesis relies on SoxC transcription factors for the survival of neural and mesenchymal progenitors.
abstract: During organogenesis, neural and mesenchymal progenitor cells give rise to many cell lineages, but their molecular requirements for self-renewal and lineage decisions are incompletely understood. In this study, we show that their survival critically relies on the redundantly acting SoxC transcription factors Sox4, Sox11 and Sox12. The more SoxC alleles that are deleted in mouse embryos, the more severe and widespread organ hypoplasia is. SoxC triple-null embryos die at midgestation unturned and tiny, with normal patterning and lineage specification, but with massively dying neural and mesenchymal progenitor cells. Specific inactivation of SoxC genes in neural and mesenchymal cells leads to selective apoptosis of these cells, suggesting SoxC cell-autonomous roles. Tead2 functionally interacts with SoxC genes in embryonic development, and is a direct target of SoxC proteins. SoxC genes therefore ensure neural and mesenchymal progenitor cell survival, and function in part by activating this transcriptional mediator of the Hippo signalling pathway.
authors.affiliation: Department of Cell Biology, and Orthopaedic and Rheumatologic Research Center, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio 44195, USA.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Bhattaram
authors.forename: Alfredo
authors.initials: A
authors.lastname: Penzo-Méndez
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Sock
authors.forename: Clemencia
authors.initials: C
authors.lastname: Colmenares
authors.forename: Kotaro J
authors.initials: KJ
authors.lastname: Kaneko
authors.forename: Alex
authors.initials: A
authors.lastname: Vassilev
authors.forename: Melvin L
authors.initials: ML
authors.lastname: Depamphilis
authors.forename: Michael
authors.initials: M
authors.lastname: Wegner
authors.forename: Véronique
authors.initials: V
authors.lastname: Lefebvre
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002453:Cell Cycle; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D024202:Electrophoretic Mobility Shift Assay; D004622:Embryo, Mammalian; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D038081:Organogenesis / Q000235:genetics / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D055751:SOXC Transcription Factors; C490793:Sox11 protein, mouse; C529443:Sox12 protein, mouse; C489990:Sox4 protein, mouse