title: Cell-free plasma DNA and purine nucleotide degradation markers following weightlifting exercise.
abstract: The present study aimed to investigate the acute effects of a single bout of high-intensive strength training on the production of cell-free plasma DNA (cf-DNA), as well as on the degradation of purine nucleotides as assessed by the concentration of xanthine (XA) and hypoxanthine (HX) in urine and serum. Twelve trained weightlifters performed six sets of six lifting exercises with 90-95% of the one repetition maximum. Blood samples and urine were obtained 1 h before training, immediately after finishing the exercise session and following 2 h of recovery. Cf-DNA, HX, and XA (in serum) significantly increased (P < 0.05-P < 0.001) immediately after heavy lifting exercise when compared with baseline levels, and significantly decreased (P < 0.05-P < 0.001) after 2 h of recovery. These results indicate that, cf-DNA and oxypurines might be relevant biomarkers for cellular damage, mechanical, energetic, and/or ischemic stress in context with exercise.
authors.affiliation: Institute of Laboratory Diagnostics, Kaiser Franz Josef Hospital, Vienna, Austria. johanna.atamaniuk@wienkav.at
authors.forename: Johanna
authors.initials: J
authors.lastname: Atamaniuk
authors.forename: Claudia
authors.initials: C
authors.lastname: Vidotto
authors.forename: Markus
authors.initials: M
authors.lastname: Kinzlbauer
authors.forename: Norbert
authors.initials: N
authors.lastname: Bachl
authors.forename: Beate
authors.initials: B
authors.lastname: Tiran
authors.forename: Harald
authors.initials: H
authors.lastname: Tschan
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002474:Cell-Free System; D004247:DNA / Q000097:blood*; D004249:DNA Damage / Q000502:physiology*; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D019271:Hypoxanthine / Q000097:blood; D010084:Oxidation-Reduction; D011685:Purine Nucleotides / Q000378:metabolism*; D014891:Weight Lifting / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D011685:Purine Nucleotides; D019271:Hypoxanthine; D004247:DNA