Improved biocompatibility of hydroxyapatite thin film prepared by aerosol deposition.
Technical development for an efficient coating of bioactive materials improves the characteristics of a fully functional implant. The aim of this study was to investigate the osteoinductive effect of a newly developed hydroxyapatite (HA)-coating technique using aerosol deposition without post-heat treatment [room temperature (RT) group] on the titanium (Ti) dental implant in vitro and in vivo, compared with that of HA coating with post-heat treatment (HT-400 group) or machined surface (control group). Cell proliferation or attachment on the HA-coated Ti surface was assessed using tetrazolium salt, WST-8 or scanning electron microscopy (SEM). Human osteoblasts (HOB) on RT group were well attached and grew alike in the control or HT-400 group. The alkaline phosphatase activity of HOB cultured on RT and HT-400 group was significantly higher than the control group (p < 0.05). Evaluation by SEM, TEM, and XRD demonstrated that aerosol deposition facilitated HA particles to form a dense and uniform HA layer in the RT group despite no post-heating. In a rabbit tibia model (n = 3), the ratios of bone implant contact and bone area in the RT group (49.88%, 86.05%) were greater than in the HT-400 group (38.82%, 77.34%) or the control (28.31%, 73.86%). The finding of this study showed that the HA coating using aerosol deposition without post-heat treatment has a good biocompatibility, and provide a promoting strategy to enhance osseointegration in the application of the dental implant.
Park
Dong-Soo
DS
Functional Ceramics Research Group, Korea Institute of Materials Science, Changwon, Gyeongnam 641-831, Republic of Korea.
Kim
In-Sook
IS
Dental Research Institute, Seoul National University, Seoul 110-768, Republic of Korea.
Kim
Hyeongil
H
Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214.
Chou
Alex Hung Kuo
AHK
Calcium Phosphate Research Lab, Department of Biomaterials, College of Dentistry, New York University, New York 10010.
Hahn
Byung-Dong
BD
Functional Ceramics Research Group, Korea Institute of Materials Science, Changwon, Gyeongnam 641-831, Republic of Korea.
Li
Long-Hao
LH
R & D Center, Dentium Co., Ltd., Suwon 443-270, Republic of Korea.
Hwang
Soon-Jung
SJ
Dental Research Institute, Seoul National University, Seoul 110-768, Republic of Korea.|Department of Oral and Maxillofacial Surgery, School of Dentistry, Brain Korea 21 2nd Program for Craniomaxillofacial Life Science, Seoul National University, Seoul 110-768, Republic of Korea.
D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000592:standards; D015921:Dental Implants; D017886:Durapatite; D006801:Humans; D020007:Implants, Experimental; D008422:Materials Testing; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology; D011817:Rabbits; D014025:Titanium
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D017886:Durapatite; D014025:Titanium