title: Sensitive high-performance anion-exchange chromatographic determination of paeoniflorin and albiflorin by pulsed amperometric detection after solid-phase extraction.
abstract: We developed a method to simultaneously determine paeoniflorin and albiflorin levels using high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPAEC-PAD). The main principle of our method includes solid-phase extraction step using Amberlite XAD-2 sorbent to remove sugars and to selectively determine glycosides by PAD. Under these conditions, the linear dynamic range was 0.01-100 microg/mL, and the albiflorin and paeoniflorin detection limits (S/N=3) were 5 and 10 pg, respectively. The intra- and inter-day precisions (RSDs) were <5.07%, and the average recoveries from Paeoniae Radix and Si-ni-san ranged from 97.12 to 101.15%. Our method showed high selectivity, high sensitivity, and good repeatability for analyzing albiflorin and paeoniflorin in oriental medicinal preparation.
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Sim
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Jeong
authors.forename: Ha-Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D000327:Adsorption; D000838:Anions / Q000737:chemistry; D001565:Benzoates / Q000032:analysis* / Q000302:isolation & purification; D001952:Bridged-Ring Compounds / Q000032:analysis* / Q000302:isolation & purification; D002241:Carbohydrates; D002852:Chromatography, Ion Exchange / Q000379:methods*; D055664:Electrochemical Techniques; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D016014:Linear Models; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D006027:Glycosides
chemical_list: D000838:Anions; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002241:Carbohydrates; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin