[Human osteoblast-like cells OS-732 intervened with alcohol and treated with liuweidihuang pill medicated serum].
OBJECTIVE
To investigate the mechanism of osteonecrosis of the femoral head caused by alcohol and the therapeutic effects of the method of invigorating the kidney.


METHODS
The osteoblast-like cells were intervened with 0.3, 0.9, 1.5 mol/L alcohol and observed by flow cytometry and light microscopy and electro microscopy. The cells intervened by 0.15 mol/L alcohol were treated with liuweidihuang pill medicated serum and observed by flow cytometry and light microscopy and electro microscopy.


RESULTS
Apoptosis rate and the degree of cell necrosis had positive correlation with the concentration and the acting time of alcohol. The polymorphism of cells wer turned into roundness, and the cellular ecphyma was shortened and thinning, ribosome and endocytoplasmic reticulum were diminished. Part of the ruffle in mitochondria was disappeared. After treatment, the cellular ecphyma was recovered and the endoplasmic reticulum and golgi apparatus were visible.


CONCLUSIONS
The osteoblast-like cells could be necrosis and apoptosis by the alcohol and were positive correlation with the intervened concentration and acting time. Chinese medicine with method of invigorating the kidney has effect on rehabilitating the cellular activity.
Liu
Wu
W
The first affiliated hospital of guangxi traditional medicine university of TCM, Nanning 530023, China. dawu6465@126.com
Fan
Yue-guang
YG
Xia
Xiong-zhi
XZ
Liu
Jian-ren
JR
Wang
Hai-bin
HB
Zeng
Yi-rong
YR
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D005271:Femur Head Necrosis / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D009336:Necrosis / Q000473:pathology; D010006:Osteoblasts / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D011817:Rabbits; D044967:Serum
D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction; D000431:Ethanol