title: Identification of three CCp genes in Babesia divergens: novel markers for sexual stages parasites.
abstract: Babesia divergens, a tick-borne protozoan parasite of red blood cells, is the main agent of bovine and human babesiosis in Europe. Very few data are available concerning its life cycle and sexual reproduction inside the tick vector, Ixodes ricinus. The aim of this study was to define some markers of the B.divergens sexual stage. An in silico post-genomic approach was used to analyze genomic, transcriptomic and proteomic data and to select specific sexual stage proteins of the related apicomplexan genus Plasmodium. Three proteins, based on sequence identity between the available genomes of Plasmodium and Babesia spp., were chosen, as members of a highly conserved and apicomplexan sexual stages specific protein family (CCp) potentially involved in adhesive functions. Degenerate primers were used to amplify and clone three B.divergens orthologs (bdccp1, bdccp2, and bdccp3) corresponding to newly identified genes in this parasite. The opportunities offered by such markers to study parasite development in its vector are discussed.
authors.affiliation: UMR INRA ONIRIS 1300 BioEpAR, ITPH Group, Nantes, France.
authors.forename: C A M
authors.initials: CA
authors.lastname: Becker
authors.forename: L
authors.initials: L
authors.lastname: Malandrin
authors.forename: D
authors.initials: D
authors.lastname: Depoix
authors.forename: T
authors.initials: T
authors.lastname: Larcher
authors.forename: P H
authors.initials: PH
authors.lastname: David
authors.forename: A
authors.initials: A
authors.lastname: Chauvin
authors.forename: E
authors.initials: E
authors.lastname: Bischoff
authors.forename: S
authors.initials: S
authors.lastname: Bonnet
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics* / Q000254:growth & development*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005819:Genetic Markers; D018884:Ixodes / Q000469:parasitology*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers; D015800:Protozoan Proteins; D037521:Virulence Factors