title: Rapid identification of Iranian Acinetobacter baumannii strains by single PCR assay using BLA oxa-51 -like carbapenemase and evaluation of the antimicrobial resistance profiles of the isolates.
abstract: The rapid identification of relevant bacterial pathogens is of utmost importance in clinical settings. The aim of this study was to test a rapid identification technique for A. baumannii strains from Tehran Hospitals and to determine the antibiotic resistance profiles of the isolates. A hundred strains of Acinetobacter spp. grown from clinical specimens were identified as A. baumannii by conventional methods. Using PCR a bla OXA-51 -like gene was detected in all A. baumannii isolates but not in other species of acinetobacter. More than half of the isolates proved resistant to a variety of antibiotics by the disk diffusion technique. The rate of resistance to gentamicin, imipenem, ampicillin-sulbactam and amikacin was determined to be 45%, 53%, 62% and 62%, respectively. Moreover, most isolates (more than 90%) showed resistance to cephalosporins. This study shows that the demonstration of the bla OXA-51-like gene is a reliable and rapid way for the presumptive identification of A. baumannii and reveals that the rate of antibiotic resistance is high in Iranian A. baumannii isolates to a variety of antibiotics.
authors.affiliation: Department of Microbiology, School of Medicine, University of Shahed, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Akbari
authors.forename: Akbari
authors.initials: A
authors.lastname: Mahdi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Niakan
authors.forename: Niakan
authors.initials: N
authors.lastname: Mohammad
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.forename: Taherikalani
authors.initials: T
authors.lastname: Morovat
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Feizabadi
authors.forename: Feizabadi
authors.initials: F
authors.lastname: Mhammad-Mahdi
authors.forename: Namam Ali
authors.initials: NA
authors.lastname: Azadi
authors.forename: Azadi
authors.initials: A
authors.lastname: Namam-Ali
authors.forename: Setareh
authors.initials: S
authors.lastname: Soroush
authors.forename: Soroush
authors.initials: S
authors.lastname: Setareh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
authors.forename: Emaneini
authors.initials: E
authors.lastname: Mohammad
authors.forename: Amir
authors.initials: A
authors.lastname: Abdolkarimi
authors.forename: Abdolkarimi
authors.initials: A
authors.lastname: Amir
authors.forename: Abbas
authors.initials: A
authors.lastname: Maleki
authors.forename: Maleki
authors.initials: M
authors.lastname: Abbas
authors.forename: Ali
authors.initials: A
authors.lastname: Hematian
authors.forename: Hematian
authors.initials: H
authors.lastname: Ali
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases