Imaging cell wall architecture in single Zinnia elegans tracheary elements.
The chemical and structural organization of the plant cell wall was examined in Zinnia elegans tracheary elements (TEs), which specialize by developing prominent secondary wall thickenings underlying the primary wall during xylogenesis in vitro. Three imaging platforms were used in conjunction with chemical extraction of wall components to investigate the composition and structure of single Zinnia TEs. Using fluorescence microscopy with a green fluorescent protein-tagged Clostridium thermocellum family 3 carbohydrate-binding module specific for crystalline cellulose, we found that cellulose accessibility and binding in TEs increased significantly following an acidified chlorite treatment. Examination of chemical composition by synchrotron radiation-based Fourier-transform infrared spectromicroscopy indicated a loss of lignin and a modest loss of other polysaccharides in treated TEs. Atomic force microscopy was used to extensively characterize the topography of cell wall surfaces in TEs, revealing an outer granular matrix covering the underlying meshwork of cellulose fibrils. The internal organization of TEs was determined using secondary wall fragments generated by sonication. Atomic force microscopy revealed that the resulting rings, spirals, and reticulate structures were composed of fibrils arranged in parallel. Based on these combined results, we generated an architectural model of Zinnia TEs composed of three layers: an outermost granular layer, a middle primary wall composed of a meshwork of cellulose fibrils, and inner secondary wall thickenings containing parallel cellulose fibrils. In addition to insights in plant biology, studies using Zinnia TEs could prove especially productive in assessing cell wall responses to enzymatic and microbial degradation, thus aiding current efforts in lignocellulosic biofuel production.
Lacayo
Catherine I
CI
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Malkin
Alexander J
AJ
Holman
Hoi-Ying N
HY
Chen
Liang
L
Ding
Shi-You
SY
Hwang
Mona S
MS
Thelen
Michael P
MP
D019659:Asteraceae / Q000033:anatomy & histology* / Q000166:cytology / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002482:Cellulose / Q000378:metabolism; D044603:Cellulosomes / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D058503:Mesophyll Cells / Q000166:cytology / Q000378:metabolism; D020894:Microfibrils / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008954:Models, Biological; D010084:Oxidation-Reduction; D058526:Plant Vascular Bundle / Q000033:anatomy & histology* / Q000166:cytology / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling
D002482:Cellulose