title: Enhancement of Candida parapsilosis catalyzing deracemization of (R,S)-1-phenyl-1,2-ethanediol to its (S)-enantiomer by a highly productive "two-in-one" resin-based in situ product removal strategy.
abstract: In this study, a highly efficient process for Candida parapsilosis-catalyzed deracemization of racemic 1-phenyl-1,2-ethanediol (PED) was described, based on a resin-based in situ substrate feeding and product removal (ISSFPR) methodology. The resin H103 was selected and used to keep the combined concentration of (R)-PED and (S)-PED at about 15g/l in aqueous phase. In the presence of 143g/l H103 resin, 50g/l of racemic substrate was converted to (S)-PED with 99.3% enantiomeric excess (ee) in 92% yield, while the initial reaction could afford an ee>99% only when the substrate concentration was 15g/l or lower. To our knowledge, this was the first report on the application of a resin-based ISSFPR technique for enhancing the efficiency of microbial stereoinversion.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors.forename: Qingsen
authors.initials: Q
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Yao
authors.initials: Y
authors.lastname: Nie
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000378:metabolism*; D005026:Ethylene Glycols / Q000737:chemistry* / Q000378:metabolism*; D016062:Porosity; D012117:Resins, Synthetic / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D005026:Ethylene Glycols; D012117:Resins, Synthetic; C031474:styrene glycol