In vitro performance assessment of new brushite-forming Zn- and ZnSr-substituted beta-TCP bone cements.
The present study investigated the in vitro performance of brushite-forming Zn- and ZnSr-substituted beta-TCP bone cements in terms of wet mechanical strength and biological response. Quantitative phase analysis and structural refinement of the powdered samples were performed by X-ray powder diffraction and Rietveld refinement technique. Initial and final setting times of the cement pastes, measured using Gilmore needles technique, showed that ZnSrCPC sets faster than ZnCPC. The measured values of the wet strength after 48 h of immersion in PBS solution at 37 degrees C showed that ZnSrCPC cements are stronger than ZnCPC cements. Human osteosarcoma-derived MG63 cell line proved the nontoxicity of the cement powders, using the resazurin metabolic assay.
Pina
S
S
Department of Ceramics and Glass Engineering, University of Aveiro, CICECO, 3810-193 Aveiro, Portugal.
Vieira
S I
SI
Health Sciences Department, Centro de Biologia Celular, University of Aveiro, 3810-193 Aveiro, Portugal.
Torres
P M C
PMC
Department of Ceramics and Glass Engineering, University of Aveiro, CICECO, 3810-193 Aveiro, Portugal.
Goetz-Neunhoeffer
F
F
Department of Mineralogy, Geozentrum Nord-Bayern, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Neubauer
J
J
Department of Mineralogy, Geozentrum Nord-Bayern, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
da Cruz E Silva
O A B
OAB
Health Sciences Department, Centro de Biologia Celular, University of Aveiro, 3810-193 Aveiro, Portugal.
da Cruz E Silva
E F
EF
Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
Ferreira
J M F
JMF
Department of Ceramics and Glass Engineering, University of Aveiro, CICECO, 3810-193 Aveiro, Portugal.
D001843:Bone Cements / Q000737:chemistry* / Q000633:toxicity; D002130:Calcium Phosphates / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D008422:Materials Testing; D012516:Osteosarcoma / Q000473:pathology; D013324:Strontium; D018675:Toxicity Tests; D015032:Zinc
D001843:Bone Cements; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D015032:Zinc; C494366:calcium phosphate, dibasic, dihydrate; D013324:Strontium