False-positive transcription-mediated amplification assay detection of West Nile virus in blood from a patient with viremia caused by an Usutu virus infection.
Detection of West Nile virus (WNV) by nucleic acid amplification technology (NAAT) is used widely to screen blood and organ donations in areas where WNV is endemic. We report a false-positive result of a WNV transcription-mediated amplification assay (TMA) in a patient with viremia that was caused by Usutu virus, a mosquito-borne flavivirus.
Gaibani
Paolo
P
Department of Haematology and Oncology L. and A. Seragnoli, Unit of Clinical Microbiology, Regional Reference Centre for Microbiological Emergencies (CRREM), S. Orsola-Malpighi Hospital, University of Bologna, 9 via G. Massarenti, 40138 Bologna, Italy.
Pierro
Anna Maria
AM
Cavrini
Francesca
F
Rossini
Giada
G
Landini
Maria Paola
MP
Sambri
Vittorio
V
D001769:Blood / Q000821:virology; D018349:Encephalitis Viruses, Japanese / Q000302:isolation & purification*; D004671:Encephalitis, Arbovirus / Q000175:diagnosis* / Q000821:virology; D005189:False Positive Reactions; D018177:Flavivirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood; D014766:Viremia; D014773:Virology / Q000379:methods*; D014901:West Nile Fever / Q000175:diagnosis / Q000821:virology; D014902:West Nile virus / Q000302:isolation & purification*
D012367:RNA, Viral