ABP1: an auxin receptor for fast responses at the plasma membrane.
Auxin-binding protein 1 (ABP1) is an auxin receptor for responses not primarily regulated by gene regulation. One fast response is protoplast swelling. By using immunological ABP1 tools we showed that the highly conserved box a is not alone important for auxin binding. Box c is another part of the auxin binding domain.1 Here we present a novel method to analyze auxin-induced, ABP1-mediated effects at the plasma membrane on single cell level in vivo. The fluorescence of FM4-64 in the plasma membrane is reduced by auxin and this response is mediated by ABP1. This method indicates a functional role of ABP1 at the plasma membrane.
Dahlke
Renate I
RI
Botanisches Institut und Botanischer Garten der Christian-Albrechts-Universität zu Kiel, Kiel, Germany. rdahlke@bot.uni-kiel.de
Luethen
Hartwig
H
Steffens
Bianka
B
D002462:Cell Membrane / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011523:Protoplasts / Q000378:metabolism*; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods; D003313:Zea mays / Q000378:metabolism*
C092350:FM 4-64; D007210:Indoleacetic Acids; D010940:Plant Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1