Towards the development of hemerythrin-based blood substitutes.
Hemerythrin is proposed as an alternative to hemoglobin-based blood substitutes. In contrast to hemoglobin, hemerythrin exhibits negligible reactivity towards oxidative and nitrosative stress agents (peroxide, nitric oxide, nitrite). Protocols for attachment of polyethylene glycol and glutaraldehyde cross-linking of Hr are described. These derivatizations appear to have favorable effects on O(2) affinity and autoxidation rates for use in blood substitutes. Based on lessons learned from hemoglobin-based blood substitutes, these derivatizations should also help limit extravasation and antigenicity of a hemerythrin-based blood substitute.
Mot
Augustin C
AC
Department of Chemistry and Chemical Engineering, "Babes-Bolyai" University, 11 Arany Janos Str, Cluj-Napoca 400028, Romania.
Roman
Alina
A
Lupan
Iulia
I
Kurtz
Donald M
DM
Silaghi-Dumitrescu
Radu
R
D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006422:Hemerythrin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008958:Models, Molecular; D009348:Nematoda / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D001802:Blood Substitutes; D006422:Hemerythrin; D006454:Hemoglobins; D011994:Recombinant Proteins; D009569:Nitric Oxide; D011092:Polyethylene Glycols; D006861:Hydrogen Peroxide; D010100:Oxygen; D005976:Glutaral