Genetic characterization and ligand specificity of the ovine Fc gamma receptor I (ovFc gamma RI).
Receptors for the Fc region of IgG (Fc gamma Rs) play a critical role in the immune system and host protection against infection. In this study, we describe the cloning, mRNA expression and IgG subclass specificity of ovine Fc gamma receptor I (ovFc gamma RI). The ovFc gamma RI cDNA contains a 1047bp open-reading frame, and is predicted to encode a 349 amino acid trans-membrane glycoprotein composed of three immunoglobulin-like domains, a trans-membrane region and a short cytoplasmic tail. The overall identity of the ovine Fc gamma RI to its cattle, human and mouse counterparts at the level of the amino acid sequence was 92%, 61% and 54%, respectively. Rosetting analysis shows that COS-7 cells were transfected with an expressional vector carrying the cDNA open-reading frame of ovFc gamma RI and expressed this receptor on the surface. Identification of ovine Fc gamma RI will aid in the understanding of molecular basis of the ovine immune system and further studies of the receptor function.
Qiao
Songlin
S
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, No.1 Nongye Road, Zhenzhou 450002, China.
Liu
Yunchao
Y
Zhang
Jiuliang
J
Yang
Suzhen
S
Wan
Bo
B
Shi
Pingling
P
Zhang
Hong
H
Guo
Junqing
J
Zhang
Gaiping
G
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D017452:Receptors, IgG / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012756:Sheep / Q000276:immunology*
D007074:Immunoglobulin G; D017452:Receptors, IgG