Additive controlled synthesis of gold nanorods (GNRs) for two-photon luminescence imaging of cancer cells.
Gold nanorods (GNRs) with a longitudinal surface plasmon resonance peak that is tunable from 600 to 1100 nm have been fabricated in a cetyl trimethylammoniumbromide (CTAB) micellar medium using hydrochloric acid and silver nitrate as additives to control their shape and size. By manipulating the concentrations of silver nitrate and hydrochloric acid, the aspect ratio of the GNRs was reliably and reproducibly tuned from 2.5 to 8. The GNRs were first coated with polyelectrolyte multilayers and then bioconjugated to transferrin (Tf) to target pancreatic cancer cells. Two-photon imaging excited from the bioconjugated GNRs demonstrated receptor-mediated uptake of the bioconjugates into Panc-1 cells, overexpressing the transferrin receptor (TfR). The bioconjugated GNR formulation exhibited very low toxicity, suggesting that it is biocompatible and potentially suitable for targeted two-photon bioimaging.
Zhu
Jing
J
Institute for Lasers, Photonics and Biophotonics, University at Buffalo, The State University of New York, Buffalo, NY 14260-4200, USA.
Yong
Ken-Tye
KT
Roy
Indrajit
I
Hu
Rui
R
Ding
Hong
H
Zhao
Lingling
L
Swihart
Mark T
MT
He
Guang S
GS
Cui
Yiping
Y
Prasad
Paras N
PN
D045744:Cell Line, Tumor; D002470:Cell Survival; D003952:Diagnostic Imaging / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D049449:Luminescence; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000473:pathology*; D017785:Photons; D012835:Silver Nitrate / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000378:metabolism
D012996:Solutions; D014168:Transferrin; D006046:Gold; D012835:Silver Nitrate; D006851:Hydrochloric Acid