Delivery of shRNA using gold nanoparticle-DNA oligonucleotide conjugates as a universal carrier.
The efficient delivery of nucleic acids into mammalian cells is a central aspect of research involving cell biology and medical applications, including the clinical treatment of genetic disorders. We report an efficient small hairpin RNA (shRNA) delivery system that utilizes a single species of gold nanoparticle-DNA oligonucleotide conjugate (AuNP-DNA oligo) as a universal carrier. In vitro synthesized shRNA that is specific to the p53 gene was efficiently delivered into HEK293 and HeLa human cell lines using an AuNP-DNA oligo. The delivery resulted in an 80-90% knockdown of p53 expression. The same AuNP-DNA oligo was also efficient for the delivery of another shRNA, which is specific to the Mcl-1 gene, as well as the repression of MCL-1 expression. The knockdown efficiency of shRNA that was delivered using an AuNP-DNA oligo was comparable with that of a liposome-based shRNA delivery method. Our results offer an alternate delivery system for shRNA that can be used on any gene of interest.
Ryou
Sang-Mi
SM
Department of Biomedical Science, College of Life Science, CHA University, Seongnam 463-836, Republic of Korea.
Kim
Sudeok
S
Jang
Hyun Hye
HH
Kim
Jae-Hong
JH
Yeom
Ji-Hyun
JH
Eom
Min Sik
MS
Bae
Jeehyeon
J
Han
Min Su
MS
Lee
Kangseok
K
D001483:Base Sequence; D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods*; D006046:Gold; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009841:Oligonucleotides / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000235:genetics
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D006046:Gold