title: Bi-modular flow characterization in tissue engineering scaffolds using computational fluid dynamics and particle imaging velocimetry.
abstract: As part of a tissue engineering (TE) therapy, cell-seeded scaffolds can be cultured in perfusion bioreactors in which the flow-mediated wall shear stress and the nutrient transport are factors that influence in vitro proliferation and osteogenic differentiation of the seeded progenitor cells. In this study both computational fluid dynamics simulations on idealized boundary conditions and circumstances and microparticle image velocimetry measurements on realistic conditions were carried out to quantify the fluid dynamic microenvironment inside a bone TE construct. The results showed that differences between actual and designed geometry and time-dependent character of the fluid flow caused a 19% difference in average fluid velocity and a 27% difference in wall shear stress between simulations and measurements. The computational fluid dynamics simulation enabled higher resolution and three-dimensional fluid flow quantification that could be quantitatively compared with a microparticle image velocimetry measurement. The coupling of numerical and experimental analysis provides a reliable and high-resolution bi-modular tool for quantifying the fluid dynamics that represent the basis to determine the relation between the hydrodynamic environment and cell growth and differentiation within TE scaffolds.
authors.affiliation: Division M3-BIORES: Measure, Model, and Manage Bioresponses, Katholieke Universiteit Leuven, Heverlee, Belgium.
authors.forename: Sebastian
authors.initials: S
authors.lastname: De Boodt
authors.forename: Silvia
authors.initials: S
authors.lastname: Truscello
authors.forename: Sezin Eren
authors.initials: SE
authors.lastname: Ozcan
authors.forename: Toon
authors.initials: T
authors.lastname: Leroy
authors.forename: Hans
authors.initials: H
authors.lastname: Van Oosterwyck
authors.forename: Daniel
authors.initials: D
authors.lastname: Berckmans
authors.forename: Jan
authors.initials: J
authors.lastname: Schrooten
mesh_terms: D019149:Bioreactors; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003198:Computer Simulation; D006801:Humans; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D012212:Rheology / Q000379:methods*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry