Direct determination of urinary lysozyme using surface plasmon resonance light-scattering of gold nanoparticles.
The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.
Wang
Xinyi
X
College of Sciences, Shenyang Agricultural University, Shenyang, China.
Xu
Yao
Y
Xu
Xiao
X
Hu
Ke
K
Xiang
Minghui
M
Li
Limei
L
Liu
Feng
F
Li
Na
N
D006046:Gold / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000652:urine*; D020349:Surface Plasmon Resonance / Q000379:methods
D006046:Gold; D009113:Muramidase