Evaluation of the epinecidin-1 peptide as an active ingredient in cleaning solutions against pathogens.
We tested the activity of epinecidin-1, a novel antimicrobial peptide structurally related to pleurocidin, in commercial cleaning solutions stored at 4 and 25 degrees C for 7 and 14 days. The peptide's activities against Enterococcus faecalis, Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa, Staphylococcus aureus, Propionibacterium acnes, and Candida albicans were measured in a minimum inhibitory concentration (MIC) determination, minimal bactericidal concentration (MBC) determination, disk diffusion test, and a count of the bacterial numbers. Exposure to epinecidn-1 in a cleaning solution following MIC value comparisons in the disk diffusion test and counts of bacterial numbers after 16, 24, 48, and 72 h suggested that bacterial numbers were much lower than those treated with only commercial cleaning solutions for all bacteria. The efficacy of the antimicrobial activities of inhibiting bacterial numbers by epinecidin-1 in cleaning solutions at a low pH and a low temperature was not affected. Given its simple structure and antimicrobial activity, epinecidin-1 may be a useful component of microbicides designed to prevent pathogen infections and/or remediate abnormal vaginal or skin flora.
Pan
Chieh-Yu
CY
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, Ilan 262, Taiwan.
Rajanbabu
Venugopal
V
Chen
Jyh-Yih
JY
Her
Guor Mour
GM
Nan
Fan-Hua
FH
D000890:Anti-Infective Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000985:Antisepsis / Q000379:methods; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D003080:Cold Temperature; D015169:Colony Count, Microbial; D004338:Drug Combinations; D004357:Drug Synergism; D029941:Fish Proteins / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D012996:Solutions; D013307:Streptomycin / Q000494:pharmacology; D013997:Time Factors
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D023181:Antimicrobial Cationic Peptides; D004338:Drug Combinations; D029941:Fish Proteins; D012996:Solutions; C521392:epinecidin-1, Epinephelus coioides; D013307:Streptomycin