title: Label-free DNA detection platform based on atomic force microscopy visualisation: characterising the molecular-recognition-triggered conformational changes of an immobilised receptor oligonucleotide probe.
abstract: Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, DNA hybridisation assay platform.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakano@cstf.kyushu-u.ac.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Nakano
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yamanouchi
authors.forename: Hisao
authors.initials: H
authors.lastname: Yoshinaga
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Soh
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Imato
mesh_terms: D004247:DNA / Q000032:analysis*; D018625:Microscopy, Atomic Force / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D012822:Silicon Dioxide; D004247:DNA