title: Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product.
abstract: In Brazil, polysaccharide-protein conjugate vaccine against Neisseria meningitidis group C (MenCPS-TT) using hydrazine-activated-tetanus toxoid (TT) as a carrier protein has been developed. Because of the toxicity of hydrazine in humans, it is necessary to monitor this substance's process control step during the vaccine production. The electroanalytical methodology was developed and validated for the determination of hydrazine during the process control of MenCPS-TT vaccine production by differential pulse polarography. The reduction potential was -0.95 V in acetone and sulphuric acid solution. The method presented linear range between 30 and 150 microgL(-1)and recovery of 93.5+/-0.8%.
authors.affiliation: Biomanguinhos/Oswaldo Cruz Foundation/FIOCRUZ, Rio de Janeiro, RJ, Brazil.
authors.forename: Renata Chagas
authors.initials: RC
authors.lastname: Bastos
authors.forename: Juliana Machado
authors.initials: JM
authors.lastname: de Carvalho
authors.forename: Ivna Alana Freitas Brasileiro
authors.initials: IA
authors.lastname: da Silveira
authors.forename: Silvana
authors.initials: S
authors.lastname: do Couto Jacob
authors.forename: Katia Christina
authors.initials: KC
authors.lastname: Leandro
mesh_terms: D006834:Hydrazines / Q000032:analysis*; D022401:Meningococcal Vaccines / Q000138:chemical synthesis*; D011048:Polarography / Q000379:methods; D013745:Tetanus Toxoid / Q000276:immunology
chemical_list: D006834:Hydrazines; D022401:Meningococcal Vaccines; D013745:Tetanus Toxoid; C410218:serogroup C meningococcal conjugate vaccine; C029424:hydrazine