title: Identification of N-glycosylation changes in the CSF and serum in patients with schizophrenia.
abstract: Schizophrenia is a major neuropsychiatric disorder that affects 2% of the population worldwide. No biochemical diagnostic tests are available, and patients must undergo lengthy clinical evaluation periods before an accurate diagnosis can be given. Blood and cerebrospinal fluid are candidates for the identification of potential biomarkers for this disease. We have identified several N-glycans that distinguish first onset, unmedicated schizophrenia patients from healthy individuals. This is the first report of the N-glycome from low abundance serum proteins and cerebrospinal fluid. The tetraantennary tetrasialylated glycan with a polylactosamine extension, A4G4LacS4, from low abundance serum proteins showed a 2-fold increase in serum from male schizophrenia patients. Gender specificity was also demonstrated as the triantennary trisialylated glycan containing the SLex epitope was increased significantly in male schizophrenia patients on both high and low abundance serum proteins. Levels of bisecting and sialylated glycans in the cerebrospinal fluid showed a general down-regulation in schizophrenia patients and a 95% positive predictive power for distinguishing patients from controls. These changes are consistent with the reported down-regulation of beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase III and beta-galactoside alpha-2,3/6-sialyltransferases in the prefrontal cortex from schizophrenia patients. These alterations in the N-glycosylation signature could be used potentially for early diagnosis and monitoring of patients after treatment.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, United Kingdom.
authors.forename: Johannes L
authors.initials: JL
authors.lastname: Stanta
authors.forename: Radka
authors.initials: R
authors.lastname: Saldova
authors.forename: Weston B
authors.initials: WB
authors.lastname: Struwe
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Byrne
authors.forename: F Markus
authors.initials: FM
authors.lastname: Leweke
authors.forename: Matthius
authors.initials: M
authors.lastname: Rothermund
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.forename: Yishai
authors.initials: Y
authors.lastname: Levin
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D001798:Blood Proteins / Q000737:chemistry; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D053858:Metabolic Networks and Pathways; D011134:Polysaccharides / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000737:chemistry; D012559:Schizophrenia / Q000097:blood* / Q000134:cerebrospinal fluid*; D012737:Sex Factors
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D006023:Glycoproteins; D011134:Polysaccharides