title: Analysis of biological demulsification process of water-in-oil emulsion by Alcaligenes sp. S-XJ-1.
abstract: A demulsifying strain (S-XJ-1) was isolated from petroleum-polluted soil and identified as Alcaligenes sp. It showed emulsion breaking ratio of 81.3% for W/O emulsion within 24h when the cell concentration was 500mg/L. Evolution of water droplets during the biological demulsification process was investigated using a Turbiscan stability analyzer and microphotography. Further investigation focused on cell surface hydrophobicity and oil-water interfacial properties. The biological demulsification process began with rapid dispersal of the cells into the oil phase and adsorption onto the oil-water interface. This occurred due to high cell surface hydrophobicity and the presence of amphiphilic compounds in the cell walls. The cells had higher interfacial activity than the emulsifier molecules, and they displaced some of the emulsifier molecules, which effectively reduced the interfacial tension gradient. As a result, the interfacial film strength decreased, the water droplets coalesced and eventually phase separation occurred.
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 20092, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wen
authors.forename: Hang
authors.initials: H
authors.lastname: Cheng
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Lu
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Xiang-Feng
authors.initials: XF
authors.lastname: Huang
mesh_terms: D000421:Alcaligenes / Q000166:cytology / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D001709:Biotechnology / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions; D009821:Oils / Q000737:chemistry*; D013500:Surface Tension; D014867:Water / Q000737:chemistry*
chemical_list: D004655:Emulsions; D009821:Oils; D014867:Water