High density lipoprotein-anionic peptide factor effect on reverse cholesterol transport in type 2 diabetic patients with and without coronary artery disease.
OBJECTIVE
To verify if HDL3 Anionic Peptide Factor (HDL3-APF) is as an apolipoprotein that promotes the reverse cholesterol transport.


METHODS
We investigated a possible association between plasma HDL3-APF concentration, cholesterol efflux from Fu5AH cells and cholesteryl ester transfer protein (CETP) activity in type 2 diabetic patients with coronary artery disease (CAD) (n=36), those without CAD (n=20), and 37 healthy subjects.


RESULTS
Plasma APF concentrations were decreased in diabetics with CAD compared to controls (p<0.01). Cellular cholesterol efflux was decreased in diabetics without and with CAD, (p<0.01 and p<0.001 respectively). CETP activity was significantly elevated in all patient groups. Multiple linear regression analysis shows that cholesterol efflux was independently and positively related only to APF concentrations in controls.


CONCLUSIONS
APF is likely to be a key independent factor for promoting cellular cholesterol efflux in healthy subjects. However this association is altered in type 2 diabetes.
Attia
Nebil
N
Laboratoire de Biochimie, UR 'Nutrition et Désordres Métaboliques' Faculté de médecine, Monastir, Tunisia. Nabil.Attia@fsb.rnu.tn
Lorec
Anne-Marie
AM
Domingo
Nicole
N
Nakbi
Amel
A
Hammami
Sonia
S
Ben Hamda
Khaldoun
K
Châaba
Raja
R
Masmoudi
Ahmed-Sleh
AS
Moulin
Philippe
P
Portugal
Henri
H
Lairon
Denis
D
Chanussot
Françoise
F
Hammami
Mohamed
M
D000328:Adult; D001692:Biological Transport; D016022:Case-Control Studies; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism; D003324:Coronary Artery Disease / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000378:metabolism; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D002784:Cholesterol