title: Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil.
abstract: OBJECTIVE
  To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in patients with dyspepsia.
  
  
  METHODS
  Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms.
  
  
  RESULTS
  Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome.
  
  
  CONCLUSIONS
  These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
authors.affiliation: Endoscopy Department, João de Barros Barreto University Hospital, 66073-000, Belém, Pará, Brazil.
authors.forename: Mônica Baraúna
authors.initials: MB
authors.lastname: Assumpção
authors.forename: Luisa Caricio
authors.initials: LC
authors.lastname: Martins
authors.forename: Hivana Patricia
authors.initials: HP
authors.lastname: Melo Barbosa
authors.forename: Katarine Antonia dos Santos
authors.initials: KA
authors.lastname: Barile
authors.forename: Sintia Silva
authors.initials: SS
authors.lastname: de Almeida
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.forename: Tereza Cristina de Oliveira
authors.initials: TC
authors.lastname: Corvelo
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001706:Biopsy; D001938:Brazil / Q000453:epidemiology; D003773:Dental Plaque / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D010437:Peptic Ulcer / Q000382:microbiology; D013270:Stomach / Q000382:microbiology*; D013272:Stomach Diseases / Q000382:microbiology*; D014510:Urease / Q000032:analysis
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D014510:Urease