Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.
We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
Hrncirova
Kristyna
K
Centre of Molecular Biology and Gene Therapy, Department of Internal Medicine-Hematooncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic. khrncirova@fnbrno.cz
Lengerova
Martina
M
Kocmanova
Iva
I
Racil
Zdenek
Z
Volfova
Pavlina
P
Palousova
Dita
D
Moulis
Mojmir
M
Weinbergerova
Barbora
B
Winterova
Jana
J
Toskova
Martina
M
Pospisilova
Sarka
S
Mayer
Jiri
J
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009090:Mucorales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009091:Mucormycosis / Q000175:diagnosis*; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D044366:Transition Temperature
D004271:DNA, Fungal