title: Virion incorporation of the herpes simplex virus type 1 tegument protein VP22 is facilitated by trans-Golgi network localization and is independent of interaction with glycoprotein E.
abstract: HSV-1 virions contain a proteinaceous layer termed the tegument that lies between the nucleocapsid and viral envelope. The molecular mechanisms that facilitate incorporation of tegument proteins are poorly characterized. The tegument protein VP22 interacts with VP16 and the cytoplasmic tail of glycoprotein E (gE). Virion incorporation of VP22 occurs independently of interaction with VP16; however, the contribution of gE binding remains undefined. Site-directed mutagenesis was used to identify VP22 mutants which abrogate interaction with gE but retain VP16 binding. Virion incorporation assays demonstrated that failure to bind gE did not abrogate VP22 packaging. A region of VP22 which binds to both VP16 and gE failed to be packaged efficiently, with wild-type levels of incorporation only attained when residues 43-86 of VP22 were present. Mutational analysis of an acidic cluster of amino acids within this region indicates that this motif facilitates trans-Golgi network (TGN) localization and optimal virion incorporation of VP22.
authors.affiliation: Fox Chase Cancer Center, Program in Immune Cell Development and Host Defense, Philadelphia, PA 19111, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: O'Regan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Brignati
authors.forename: Michael A
authors.initials: MA
authors.lastname: Murphy
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Bucks
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Courtney
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011485:Protein Binding; D021381:Protein Transport; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D021601:trans-Golgi Network / Q000502:physiology*
chemical_list: D000596:Amino Acids; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C044768:glycoprotein E, herpes simplex virus type 1; C073413:herpes simplex virus type 1 protein VP22