title: Development and validation of a liquid chromatography-mass spectrometry method for the determination of verticinone in rat plasma and its application to pharmacokinetic study.
abstract: We have developed and validated a sensitive liquid chromatography-electrospray ionization-mass spectrometric (LC-ESI-MS) method for the quantification of verticinone, a major active constituent from Fritillaria hupehensis Hsiao et KC Hsia., in rat plasma. Verticinone and the internal standard (IS), hupehenine, were extracted from plasma samples by a simple liquid-liquid extraction with ethyl acetate after being alkalified by 1M ammonia hydroxide. Chromatographic separation was achieved on a C(18) column using a gradient elution program with methanol and water as the mobile phase. The detection was performed by selected ion monitoring (SIM) mode via positive electrospray ionization (ESI) interface. The lower limit of quantification (LLOQ) was 0.1 ng/mL. The calibration curves were linear (r(2)>0.998) over the concentration range of 0.1-200 ng/mL. Within- and between-run precision was less than 6.5% and accuracy was within +/-10.7%. The validated method was applied to the pharmacokinetic study of verticinone in rats after a single oral administration of 1 mg/kg.
authors.affiliation: Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Jizhou
authors.initials: J
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002595:Cevanes / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000470:Alkaloids; D002595:Cevanes; C532925:hupehenine; C014242:verticine