Regulatory T-cells have a prominent role in the immune modulated vaccine response by specific oligosaccharides.
Regulatory T-cells are increasingly important in vaccine strategies. In a Flu-vaccination model the role of CD4(+)CD25(+)Foxp3(+) regulatory T-cells (Tregs) and the immune modulation by orally supplied prebiotic oligosaccharides consisting of scGOS/lcFOS/pAOS, were assessed using anti-CD25 (PC61) mediated depletion studies. As expected, in C57BL/6J mice the Flu-vaccination resulted in significantly (p<0.001) increased DTH responses when receiving scGOS/lcFOS/pAOS. In addition, increased T-bet expression of activated CD4(+) T-cells was detected compared to placebo. In vivo depletion of CD25(+) Tregs significantly (p<0.05) increased basal DTH responses, indicating the suppressive function of these CD25(+) Tregs normally present. Surprisingly, in vivo Tregs depletion diminished scGOS/lcFOS/pAOS induced immune modulation completely to control levels (p<0.05). Although no difference in number, percentage or activation of Tregs could be determined after scGOS/lcFOS/pAOS supplementation, changes in Treg function still remains to be investigated. In conclusion, CD25(+) Tregs have an important role in modulated Flu-vaccine responses induced by scGOS/lcFOS/pAOS.
van't Land
Belinda
B
Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, The Netherlands. B.vantland@uu.nl
Schijf
Marcel
M
van Esch
Betty C A M
BC
van Bergenhenegouwen
Jeroen
J
Bastiaans
Jacqueline
J
Schouten
Bastiaan
B
Boon
Louis
L
Garssen
Johan
J
D000818:Animals; D007252:Influenza Vaccines / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D056692:Prebiotics; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D007252:Influenza Vaccines; D053645:Interleukin-2 Receptor alpha Subunit; D009844:Oligosaccharides; D056692:Prebiotics