title: The effect of orexin-A on the pathological mechanism in the rat focal cerebral ischemia.
abstract: Orexin-A is a neuropeptide involved in the control feeding, arousal or sleep behavior in the hypothalamus. In the present study, the cortex and lateral hypothalamus of rats subjected to middle cerebral artery occlusion (MCAO)-reperfusion brain injury were examined by double immunofluorescence staining. The number of orexin-A-expressing neurons in the non-ischemic side was significantly lower than the ischemic side. Next, orexin-A was administered intracerebroventricularly followed by the induction of MCAO-reperfusion injury. Administration of orexin-A at 0.3nmol significantly reduced the brain infarct area. The results suggested that orexin-A alters the pathological mechanisms involved in brain ischemia and has a neuroprotective effect.
authors.affiliation: Department of Neurology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan. eieikitamura0622@yahoo.co.jp
authors.forename: Eiji
authors.initials: E
authors.lastname: Kitamura
authors.forename: Junichi
authors.initials: J
authors.lastname: Hamada
authors.forename: Naomi
authors.initials: N
authors.lastname: Kanazawa
authors.forename: Junko
authors.initials: J
authors.lastname: Yonekura
authors.forename: Ray
authors.initials: R
authors.lastname: Masuda
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sakai
authors.forename: Hideki
authors.initials: H
authors.lastname: Mochizuki
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020520:Brain Infarction / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D007276:Injections, Intraventricular / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism* / Q000494:pharmacology; D000068797:Orexins; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D000068797:Orexins