Determination of non-steroidal anti-inflammatory drugs residues in animal muscles by liquid chromatography-tandem mass spectrometry.
A confirmatory method for the determination of residues of nine non-steroidal anti-inflammatory drugs and one metabolite in animal muscles has been developed. After enzymatic hydrolysis samples were extracted with acetonitrile and cleaned up using alumina and C(18) SPE cartridges. Liquid chromatography-tandem mass spectrometry was used for the separation and determination of analytes. The method was validated in bovine muscles, according to the Commission Decision 2002/657/EC criteria. Applicability of the method in the analysis of swine, horse and chicken muscles was checked by precision and recovery experiment. The influence of matrix effect on the quantification of non-steroidal anti-inflammatory drugs residues was investigated. The method was used for the confirmation of phenylbutazone and oxyphenbutazone in horse muscle sample.
Jedziniak
Piotr
P
National Veterinary Research Institute, Department of Pharmacology and Toxicology, Al. Partyzantow 57, 24-100 Pulawy, Poland. jedzi@piwet.pulawy.pl
Szprengier-Juszkiewicz
Teresa
T
Olejnik
Małgorzata
M
Zmudzki
Jan
J
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D006736:Horses; D018482:Muscle, Skeletal / Q000737:chemistry*; D010113:Oxyphenbutazone / Q000032:analysis; D010653:Phenylbutazone / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010653:Phenylbutazone; D010113:Oxyphenbutazone