title: Oocyte glutathione and fertilisation outcome of Macaca nemestrina and Macaca fascicularis in in vivo- and in vitro-matured oocytes.
abstract: Fertilisation and development of IVM non-human primate oocytes is limited compared with that of in vivo-matured (IVO) oocytes. The present study describes the IVM of macaque oocytes with reference to oocyte glutathione (GSH). Timing of maturation, comparison of IVM media and cysteamine (CYS) supplementation as a modulator of GSH were investigated. A significantly greater proportion of oocytes reached MII after 30 h compared with 24 h of IVM. Following insemination, IVM oocytes had a significantly lower incidence of normal fertilisation (i.e. 2PN = two pronuclei and at least one polar body) and a higher rate of abnormal fertilisation (1PN = one pronucleus and at least one polar body) compared with IVO oocytes. Immunofluorescence of 1PN zygotes identified incomplete sperm head decondensation and failure of male pronucleus formation as the principal cause of abnormal fertilisation in IVM oocytes. The IVO oocytes had significantly higher GSH content than IVM oocytes. Cumulus-denuded oocytes had significantly lower GSH following IVM compared with immature oocytes at collection. Cysteamine supplementation of the IVM medium significantly increased the GSH level of cumulus-intact oocytes and reduced the incidence of 1PN formation, but did not improve GSH levels of the denuded oocyte. Suboptimal GSH levels in macaque IVM oocytes may be related to reduced fertilisation outcomes.
authors.affiliation: Washington National Primate Research Center, University of Washington, Seattle, WA 98121, USA. ecurnow@wanprc.org
authors.forename: E C
authors.initials: EC
authors.lastname: Curnow
authors.forename: J P
authors.initials: JP
authors.lastname: Ryan
authors.forename: D M
authors.initials: DM
authors.lastname: Saunders
authors.forename: E S
authors.initials: ES
authors.lastname: Hayes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro; D005455:Fluorescent Antibody Technique; D005978:Glutathione / Q000032:analysis*; D008252:Macaca fascicularis; D008254:Macaca nemestrina; D009865:Oocytes / Q000737:chemistry* / Q000254:growth & development*; D013997:Time Factors
chemical_list: D003470:Culture Media; D005978:Glutathione