An experimental study of use of absorbable plate in combination with self-setting α-tricalcium phosphate for orthognathic surgery.
OBJECTIVE
The purpose of this study was to histologically and immunohistochemically evaluate bone formation using both self-setting α-tricalcium phosphate (α-TCP; Biopex) and absorbable plate (Super Fixsorb-MX) in rabbit cranium bone.


METHODS
Twelve adult male Japanese white rabbits (12-16 wk, 2.5-3.0 kg) were used. The surgical defects were made in the nasal bone of a rabbit, and Biopex was implanted in the left side and no material in the right side. Two-hole absorbable plate and 2 screws (Super Fixsorb-MX) were fixed across the defect in each side. The rabbits were killed at 1, 4, 12, and 24 weeks after surgery, and formalin-fixed specimens were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with bone morphogenetic protein 2 (BMP-2) antibodies. Finally, these were evaluated microscopically.


RESULTS
New bone formation was observed in the region of absorbable plate and nasal membrane after >4 weeks. The area of new bone with Biopex was significantly larger than that of the control side after 1, 4, and 12 weeks (P < .05). The number of BMP-2-stained cells in the experimental side was significantly larger than in the control side after 4 and 12 weeks (P < .05).


CONCLUSIONS
This study suggests that the use of absorbable plate (Super Fixsorb-MX) in combination with Biopex could be useful and that both of Super Fixsorb-MX and Biopex could provide adequate bone regeneration.
Okabe
Katsuhiko
K
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kanazawa University, Kanazawa, Japan.
Ueki
Koichiro
K
Marukawa
Kohei
K
Mukozawa
Aya
A
Miyazaki
Mao
M
Nakagawa
Kiyomasa
K
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis; D001860:Bone Plates; D001861:Bone Regeneration / Q000187:drug effects; D001863:Bone Screws; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002452:Cell Count; D002809:Chondroitin Sulfates / Q000627:therapeutic use; D004396:Coloring Agents; D006882:Hydroxyapatites / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D009295:Nasal Bone / Q000473:pathology / Q000601:surgery; D056948:Orthognathic Surgical Procedures / Q000295:instrumentation / Q000379:methods*; D010006:Osteoblasts / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D013386:Succinates / Q000627:therapeutic use; D013997:Time Factors
D001672:Biocompatible Materials; C481019:Biopex; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004396:Coloring Agents; D006882:Hydroxyapatites; D013386:Succinates; C485828:alpha-tricalcium phosphate; D002809:Chondroitin Sulfates