Gaussia luciferase reporter assay for assessment of gene delivery systems in vivo.
OBJECTIVE
To develop an alternative method for assessment of gene delivery systems in vivo.


METHODS
Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected.


RESULTS
As little as 10 muL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.


CONCLUSIONS
Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.
Chen
Feng
F
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
Xu
Zhen
Z
Lu
Jiang
J
Lü
Xiang
X
Mu
Wen-li
WL
Wang
Ya-jun
YJ
Liu
De-pei
DP
Liang
Chih-chuan
CC
D000818:Animals; D028023:Arecaceae / Q000201:enzymology*; D002460:Cell Line; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics*; D051379:Mice
D008156:Luciferases