title: HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star).
abstract: An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV-Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC(25)=268 microg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC(25)=411 microg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Murcia, Spain.
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: David M
authors.initials: DM
authors.lastname: Pereira
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: João
authors.initials: J
authors.lastname: Botelho
authors.forename: Teresa
authors.initials: T
authors.lastname: Mouga
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001274:Atmospheric Pressure; D002338:Carotenoids / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D004354:Drug Screening Assays, Antitumor; D015394:Molecular Structure; D051381:Rats; D013215:Starfish / Q000737:chemistry*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D002338:Carotenoids