title: [The screening and identification of Apolipoprotein A-II from serum differential proteins in hepatocellular carcinoma patients].
abstract: OBJECTIVE
  To screen differential proteins in serum from hepatocellular carcinoma (HCC) patients by Proteomic Technology and to purify and identify them.
  
  
  METHODS
  Surface enhanced laser desorption Ionization time of flight-mass spectrum (SELDI-TOF-MS) was employed to screen differential proteins in serum from 33 HCC patients and 33 control cases, and then to purify and identify them using isoelectric precipitation, Tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) and high performance liquid chromatography tandem Mass Spectrum (HPLC-MS).
  
  
  RESULTS
  65 protein peaks in the range of relative molecular weight from 2,000 to 10,000 were found significant difference (P less than 0.05) between the patient group and control group. Based on these differential protein peaks, diagnostic model for HCC detection was established and its sensitivity and specificity were 100% and 96.97% respectively. Proteins with 8,706.5 and 8,579.2 relative molecular weights (the t value was 2.562 and 2.783 respectively, and P value was 0.013 and 0.015 respectively) out of the 65 differential proteins were purified and identified, and then recognized as Apolipoprotein AII (Apo AII).
  
  
  CONCLUSIONS
  Apo AII is probably a differential protein of HCC and maybe related to the pathogenesis of HCC.
authors.affiliation: Guangxi Center for Disease Control and Prevention, Nanning 530028, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.forename: Bing-Jin
authors.initials: BJ
authors.lastname: Nong
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
mesh_terms: D016633:Apolipoprotein A-II / Q000302:isolation & purification*; D001798:Blood Proteins / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000097:blood*; D016022:Case-Control Studies; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C115794:APOA2 protein, human; D016633:Apolipoprotein A-II; D001798:Blood Proteins