Effect of stem cells and fibrin concentration on the vascularization of the Medpor orbital implant.
BACKGROUND
To determine the effect of adipose-derived adult stem cells (ADASCs) and optimal concentration of fibrin on fibrovascular ingrowth into porous polyethylene orbital implants (Medpor).


METHODS
Medpor sheet treated with O.25% fibrin only and ADASCs in mixtures containing fibrin (0.25%, 0.5% or 1.25%) were applied to a Medpor sheet and implanted in the back of each of 20 athymic nude mice. After 10 days, implants were removed and observed for fibrovascularization and stability. Haemoglobin, collagen and cellular DNA content were determined in quantitative assays.


RESULTS
Haemoglobin, collagen and cellular DNA levels were significantly higher in ADASC group than in the cell-free implant (0.25% fibrin only) group (P < 0.01). The level of haemoglobin and collagen content was significantly higher in the ADASC + 0.5% fibrin group among the ADASC and fibrin mixtures (P < 0.01).


CONCLUSIONS
ADASCs significantly improved fibrovascularization on Medpor compared with implants alone. Fibrin, used together with ADASCs to potentiate fibrovascularization, was most effective at concentrations of 0.5%.
Lee
Sul-Gee
SG
Department of Ophthalmology, Inje University, Busan, Republic of Korea.
Yang
Jae-Wook
JW
Park
Sae-Gwang
SG
Yang
Young-Il
YI
D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D005337:Fibrin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D020273:Orbital Implants; D020959:Polyethylene; D011095:Polyethylenes / Q000494:pharmacology*
D001672:Biocompatible Materials; C084562:Medpor; D011095:Polyethylenes; D005337:Fibrin; D020959:Polyethylene