Correlation of mineral density and elastic modulus of natural enamel white spot lesions using X-ray microtomography and nanoindentation.
Our objectives were to correlate the mineral density (MD) and elastic modulus (E) of natural white spot lesions (WSLs) and compare them with analytical and numerical models. Five natural WSLs from four extracted sound premolar teeth were scanned at a voxel size of 7.6μm using a desktop X-ray microtomography (XRMT) system. Five hydroxyapatite phantoms with densities ranging from 1.52 to 3.14gcm⁻³ were used as calibration standards for each scan. MD throughout the WSLs was quantified using an MD calibration equation derived from hydroxyapatite phantoms. Subsequently, teeth were cross-sectioned and the E modulus was measured systematically across the WSLs at intervals of 25 and 50μm using nanoindentation. The MD and E modulus of WSLs correlated well. The relationship may be expressed as E=E⁰exp(-bP) (R²=0.952) with E⁰ the elastic modulus of the fully dense material, P the porosity and b a constant. The results for sound enamel were compared with Spears model. The limitation of Spears model to the WSLs is discussed and an alternative model developed by Rice for porous materials is proposed. Clinical implications of this work for quantifying de-/remineralization of teeth are pointed out. We conclude that XRMT can be utilized to extrapolate the E modulus of WSLs. This provides a basis for non-destructive, longitudinal analysis of WSLs in de-/remineralization studies of enamel.
Huang
Tiffany T Y
TT
Biomaterials Science Research Unit, Faculty of Dentistry, University of Sydney, NSW, Australia.
He
Li-Hong
LH
Darendeliler
M Ali
MA
Swain
Michael V
MV
D000293:Adolescent; D015519:Bone Density / Q000502:physiology*; D003731:Dental Caries / Q000000981:diagnostic imaging* / Q000473:pathology / Q000503:physiopathology*; D003743:Dental Enamel / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D055119:Elastic Modulus / Q000502:physiology*; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D055114:X-Ray Microtomography