title: Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR.
abstract: Laboratory bred female Aedes aegypti (L.) was used to determine sensitivity of multiplex PCR for detecting human blood meal. Human blood DNA was detected in live fully fed mosquitoes until 3 days after blood feeding, and for 4 weeks when stored at -20 degrees C. Among 890 field caught female mosquito samples examined for vertebrate DNA by multiplex PCR, results were positive for human, pig, dog, cow and mixture of 2 host DNA at 86.1, 3.4, 2.1, 1.0 and 3.6%, respectively, while 3.9% of the samples were negative. Blood feeding pattern must be considered when mosquito control strategies become employed.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. padet.s@chula.ac.th
authors.forename: Padet
authors.initials: P
authors.lastname: Siriyasatien
authors.forename: Theerakamol
authors.initials: T
authors.lastname: Pengsakul
authors.forename: Veerayuth
authors.initials: V
authors.lastname: Kittichai
authors.forename: Atchara
authors.initials: A
authors.lastname: Phumee
authors.forename: Sakchai
authors.initials: S
authors.lastname: Kaewsaitiam
authors.forename: Usavadee
authors.initials: U
authors.lastname: Thavara
authors.forename: Apiwat
authors.initials: A
authors.lastname: Tawatsin
authors.forename: Preecha
authors.initials: P
authors.lastname: Asavadachanukorn
authors.forename: Mir S
authors.initials: MS
authors.lastname: Mulla
mesh_terms: D000330:Aedes / Q000502:physiology*; D000818:Animals; D001800:Blood Specimen Collection; D002417:Cattle; D004247:DNA / Q000097:blood*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007303:Insect Vectors; D016133:Polymerase Chain Reaction; D013552:Swine
chemical_list: D004247:DNA