title: Common carp have two subclasses of bonyfish specific antibody IgZ showing differential expression in response to infection.
abstract: Immunoglobulin heavy chains identified in bony fish are broadly classified into three classes namely IgM, IgD and IgZ. The most recently described isotype is IgZ, a teleosts-fish specific isotype that shows variations in gene structure across teleosts. In this study we have identified two IgZ subclasses in common carp. IgZ1 is a four constant heavy chain domains containing antibody isolated across teleosts and IgZ2 is a two constant domains containing heavy chain chimera with a μ1 and ζ4 domain. Sequence analyses suggest that these subtypes are expressed from two separate genomic loci. Expression analyses show that IgZ1 is more abundant in systemic organs and IgZ2 chimera is preferentially expressed at mucosal sites. The basal expression level of IgM in fish is much higher than of the other isotypes. We show that IgZ1 expression in systemic and mucosal organs is responsive to blood parasites, while mucosal parasite infection induces IgM and IgZ2 gene expression. This report is the first to show differential expression of the IgZ variants in response to pathogens and suggests that the IgZ subtypes in carps may have mutually exclusive humoral functions.
authors.affiliation: Faculty of Agriculture, University of Miyazaki, Gakuen kibanadai nishi 1-1, Miyazaki 889-2192, Japan.
authors.forename: Sogabe
authors.initials: S
authors.lastname: Ryo
authors.forename: Ruud H M
authors.initials: RH
authors.lastname: Wijdeven
authors.forename: Anuj
authors.initials: A
authors.lastname: Tyagi
authors.forename: Trudi
authors.initials: T
authors.lastname: Hermsen
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kono
authors.forename: Indrani
authors.initials: I
authors.lastname: Karunasagar
authors.forename: Jan H W M
authors.initials: JH
authors.lastname: Rombout
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakai
authors.forename: B M Lidy
authors.initials: BM
authors.lastname: Verburg-van Kemenade
authors.forename: Ram
authors.initials: R
authors.lastname: Savan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology; D006374:Helminthiasis, Animal / Q000235:genetics / Q000276:immunology*; D006376:Helminths / Q000276:immunology / Q000472:pathogenicity; D056724:Immunity, Humoral; D007132:Immunoglobulin Isotypes / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology / Q000469:parasitology; D008969:Molecular Sequence Data; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D014345:Trypanosoma / Q000276:immunology / Q000472:pathogenicity; D014352:Trypanosomiasis / Q000235:genetics / Q000276:immunology*; D014733:Vibrio / Q000276:immunology / Q000472:pathogenicity; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D007132:Immunoglobulin Isotypes