title: Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats.
abstract: Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adipose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incorporation of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supplementation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers.
authors.affiliation: REQUIMTE, Dep. Química, FCT-UNL, 2829-516 Caparica, Portugal.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Martins
authors.forename: Paula A
authors.initials: PA
authors.lastname: Lopes
authors.forename: Susana V
authors.initials: SV
authors.lastname: Martins
authors.forename: Ana
authors.initials: A
authors.lastname: Madeira
authors.forename: Nuno C
authors.initials: NC
authors.lastname: Santos
authors.forename: José A M
authors.initials: JA
authors.lastname: Prates
authors.forename: Teresa F
authors.initials: TF
authors.lastname: Moura
authors.forename: Graça
authors.initials: G
authors.lastname: Soveral
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D009765:Obesity / Q000378:metabolism*; D051381:Rats; D011924:Rats, Zucker
chemical_list: D004042:Dietary Fats, Unsaturated; D044243:Linoleic Acids, Conjugated