Knockdown of Cyclophilin D Gene by RNAi Protects Rat from Ischemia/ Reperfusion-Induced Renal Injury.
OBJECTIVE
Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury.


METHODS
To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury.


RESULTS
Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury.


CONCLUSIONS
Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury.
Hu
Wei
W
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
Chen
Zhiqiang
Z
Ye
Zhangqun
Z
Xia
Ding
D
Xia
Zhongyu
Z
Ma
Jiefeng
J
Zhu
Min
M
Chen
Gang
G
D000818:Animals; D015687:Cell Hypoxia / Q000235:genetics; D002460:Cell Line; D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins / Q000172:deficiency / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D008297:Male; D034622:RNA Interference / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000517:prevention & control*
D000081406:Peptidyl-Prolyl Isomerase F; D021983:Cyclophilins