title: Cellular receptors, differentiation and endocytosis requirements are key factors for type I IFN response by human epithelial, conventional and plasmacytoid dendritic infected cells by measles virus.
abstract: While the antiviral response during measles virus (MeV) infection is documented, the contribution of the hosting cell type to the type I interferon (IFN-alpha/beta) response is still not clearly established. Here, we report that a signature heterogeneity of the IFN-alpha/beta response according to the cell type. The MeV tropism dictated by the expression of appropriate cellular receptor appeared to be crucial for epithelial cells. For conventional DCs (cDCs), the maturation state played a prominent role. In response to both wild type MeV isolates and laboratory/vaccine strains, immature cDCs produced higher levels of IFN-alpha than mature cDCs, despite the reduced expression levels of both CD46 and CD150 receptors by the former ones. While in epithelial cells and cDCs the MeV transcription was required to activate the IFN-alpha/beta response, plasmacytoid DCs (pDCs) rapidly produced large amounts of IFN-alpha mostly independently of the viral infection cycle. This argues for a significant contribution of pDCs in response to MeV infection and/or vaccination.
authors.affiliation: Université de Lyon, INSERM U851, 21 Avenue Tony Garnier, Lyon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Duhen
authors.forename: Florence
authors.initials: F
authors.lastname: Herschke
authors.forename: Olga
authors.initials: O
authors.lastname: Azocar
authors.forename: Johan
authors.initials: J
authors.lastname: Druelle
authors.forename: Sébastien
authors.initials: S
authors.lastname: Plumet
authors.forename: Chistine
authors.initials: C
authors.lastname: Delprat
authors.forename: Stéphane
authors.initials: S
authors.lastname: Schicklin
authors.forename: T Fabian
authors.initials: TF
authors.lastname: Wild
authors.forename: Chantal
authors.initials: C
authors.lastname: Rabourdin-Combe
authors.forename: Denis
authors.initials: D
authors.lastname: Gerlier
authors.forename: Hélène
authors.initials: H
authors.lastname: Valentin
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology; D004705:Endocytosis; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008457:Measles / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D051916:Membrane Cofactor Protein / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology; D011991:Receptors, Virus / Q000276:immunology*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D056189:Viral Tropism
chemical_list: D015703:Antigens, CD; D007370:Interferon Type I; D051916:Membrane Cofactor Protein; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1