Triggering cell detachment from patterned electrode arrays by programmed subcellular release.
Programmed subcellular release is an in vitro technique for the quantitative study of cell detachment. The dynamics of cell contraction are measured by releasing cells from surfaces to which they are attached with spatial and temporal control. Release of subcellular regions of cells is achieved by plating cells on an electrode array created by standard microfabrication methods. The electrodes are then biochemically functionalized with an arginine-glycine-aspartic acid (RGD)-terminated thiol. Application of a voltage pulse results in electrochemical desorption of the RGD-terminated thiols, triggering cell detachment. This method allows for the study of the full cascade of events from detachment to subsequent subcellular reorganization. Fabrication of the electrode arrays may take 1-2 d. Preparation for experiments, including surface functionalization and cell plating, can be completed in 10 h. A series of cell release experiments on one device may last several hours.
Wildt
Bridget
B
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA. wirtz@jhu.edu
Wirtz
Denis
D
Searson
Peter C
PC
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry; D051379:Mice; D008839:Microelectrodes; D008856:Microscopy, Fluorescence; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods*; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry
D009842:Oligopeptides; D011092:Polyethylene Glycols; D006046:Gold; C047981:arginyl-glycyl-aspartic acid