[Studies on chemistry component and the biological activity of petroleum ether extraction from pre-and post-processed of Cornus officinalis].
OBJECTIVE
To investigate substance basis for improving pharmacodynamics by comparing the chemical constituents of petroleum ether extraction from crude and processed Cornus officinalis and the effects on the immunologic function of mice with immunosuppression induced.


METHODS
The volatile components in petroleum ether extraction were analyses by GC-MS. Non-specific immune function was determined by cleaning carbon particle index K, Swallow index a, spleen index and thymus index. The specific humoral immune function was evaluated by detecting the content of serum hemolysin.


RESULTS
The chemical constituents of petroleum ether extraction of Cornus officinalis before and after being processed had significant changes. After being processed, Vitamin E increased by 46.6%, linoleic acid increased by 18.3% and methyl linen increased by 30.9%. The extraction increased clearance rate of charcoal carbon particles index K, swallow index alpha, spleen index, thymus index and the level of serum hemolysin.


CONCLUSIONS
The extraction can markedly improve non-specific immune function and the specific humoral immune function which are active sites of improving immunologic function and post-processed is better. Substance basis could be Vitamin E, Linoleic acid, methyl linen and so on.
Li
Guan-ye
GY
Department of Chemistry, Nanjing Agricutural University, Nanjing 210095, China.
Yao
Yun-xiang
YX
Ding
Xia
X
D000818:Animals; D029768:Cornus / Q000737:chemistry*; D003520:Cyclophosphamide / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006460:Hemolysin Proteins / Q000097:blood; D007109:Immunity / Q000187:drug effects*; D007165:Immunosuppression Therapy; D019787:Linoleic Acid / Q000032:analysis; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects; D014810:Vitamin E / Q000032:analysis
D006460:Hemolysin Proteins; D010936:Plant Extracts; D014810:Vitamin E; D003520:Cyclophosphamide; D019787:Linoleic Acid