The Escherichia coli glycophage display system.
We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylation.
Dürr
Clemens
C
Institute of Microbiology, Department of Biology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.
Nothaft
Harald
H
Lizak
Christian
C
Glockshuber
Rudi
R
Aebi
Markus
M
D003090:Coliphages / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000821:virology; D006031:Glycosylation