title: [The investigation of Entamoeba gingivalis and Trichomonas tenax in a group of patients with periodontal disease].
abstract: The oral cavity is suitable for invasion of many microorganisms. Entamoeba gingivalis (E.gingivalis) and Trichomonas tenax (T.tenax) settle in the oral cavity of patients with poor oral hygiene and gingival disease. In the present study, two slide specimens were prepared from the cole region of the teeth of 46 persons for investigation of the parasites. One of the slide specimens was dried in the air while the other one put into fixative and they were stained with trichrome and Giemsa stains. The two staining methods were used for 36 samples and only Giemsa, for 10 samples. E. gingivalis was positive in 7 (19.44%) out of 36 samples stained by the trichrome stain while T. tenax was positive in one (2.17%) out of 46 samples stained by Giemsa stain. Parasitic infections were found to be positive in seven (21.2%) specimen from 33 patients with periodontal disease and in one (7.69%) specimen from 13 healthy controls. Dental policlinics are generally far from parasitology laboratories and microscopical wet mount examination can not be performed. Therefore dentists can send the specimens and have the parasites diagnosed with Giemsa and trichrome staining methods as an alternative to wet mount examination.
authors.affiliation: Ege Universitesi Diş Hekimliği Fakültesi Oğrencisi, Izmir, Turkey.
authors.forename: Moin
authors.initials: M
authors.lastname: Abualqomsaan
authors.forename: Seray Ozensoy
authors.initials: SO
authors.lastname: Töz
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Yolasiğmaz
authors.forename: Nevin
authors.initials: N
authors.lastname: Turgay
mesh_terms: D000328:Adult; D001391:Azo Compounds; D001399:Azure Stains; D016022:Case-Control Studies; D004396:Coloring Agents; D004747:Entamoeba / Q000145:classification / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D004801:Eosine Yellowish-(YS); D005260:Female; D005891:Gingivitis / Q000453:epidemiology / Q000469:parasitology; D006801:Humans; D008297:Male; D008739:Methyl Green; D008875:Middle Aged; D010510:Periodontal Diseases / Q000150:complications* / Q000469:parasitology; D010518:Periodontitis / Q000453:epidemiology / Q000469:parasitology; D014244:Trichomonas / Q000145:classification / Q000302:isolation & purification; D014245:Trichomonas Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D014421:Turkey / Q000453:epidemiology
chemical_list: D001391:Azo Compounds; D001399:Azure Stains; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)