A photoactivatable marker protein for pulse-chase imaging with superresolution.
IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).
Fuchs
Jochen
J
Institute of Applied Physics and Center for Functional Nanostructures, Karlsruhe Institute of Technology, Karlsruhe, Germany.
Böhme
Susan
S
Oswald
Franz
F
Hedde
Per Niklas
PN
Krause
Maike
M
Wiedenmann
Jörg
J
Nienhaus
G Ulrich
GU
D015415:Biomarkers; D003116:Color; D003952:Diagnostic Imaging / Q000379:methods; D005453:Fluorescence; D008164:Luminescent Proteins / Q000528:radiation effects*; D008853:Microscopy / Q000379:methods*; D055668:Photochemical Processes; D011868:Radioisotopes
D015415:Biomarkers; D008164:Luminescent Proteins; D011868:Radioisotopes