title: EFFECT OF PHALLOIDIN ON FILAMENTS POLYMERIZED FROM HEART MUSCLE ADP-ACTIN MONOMERS.
abstract: The effect of phalloidin on filaments polymerized from ADP-actin monomers of the heart muscle was investigated with differential scanning calorimetry. Heart muscle contains alpha-skeletal and alpha-cardiac actin isoforms. In the absence of phalloidin the melting temperature was 55 degrees C for the alpha-cardiac actin isoform and 58 degrees C for the alpha-skeletal one when the filaments were generated from ADP-actin monomers. After the binding of phalloidin the melting temperature was isoform independent (85.5 degrees C). We concluded that phalloidin stabilized the actin filaments of alpha-skeletal and alpha-cardiac actin isoforms to the same extent when they were polymerized from ADP-actin monomers.
authors.affiliation: University of Pécs, Faculty of Medicine, Department of Biophysics, Szigeti str. 12, Pécs 7624, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vig
authors.forename: Réka
authors.initials: R
authors.lastname: Dudás
authors.forename: Tünde
authors.initials: T
authors.lastname: Kupi
authors.forename: J
authors.initials: J
authors.lastname: Orbán
authors.forename: G
authors.initials: G
authors.lastname: Hild
authors.forename: D
authors.initials: D
authors.lastname: Lőrinczy
authors.forename: M
authors.initials: M
authors.lastname: Nyitrai