Formation of a combined Ca/Cd toxicity on lifespan of nematode Caenorhabditis elegans.
We investigated the possible formation of combined toxicity from Ca/Cd exposure on nematode lifespan. Ca exposure at concentrations more than 1.56 mM significantly reduced lifespan, accelerated aging-related declines, and induced severe stress response in wild-type nematodes. Combined Ca (25 mM)/Cd (200 microM) exposure decreased the lifespans compared to Cd (200 microM) exposure; whereas no lifespan differences were found between Ca (1.56 mM)/Cd (200 microM) exposure and Cd (200 microM) exposure. Combined Ca (25 mM)/Cd (200 microM) exposure caused a more significant induction of hsp-16.2::gfp expression, and a more severe increase in oxidative damage than Cd (200 microM) exposure. Moreover, mutation of mev-1, encoding a subunit of succinate dehydrogenase cytochrome b, enhanced the combined Ca/Cd toxicity on lifespan. Furthermore, mutation of daf-16, encoding a fork-head-family transcription factor, enhanced the combined Ca/Cd toxicity on lifespan, and mutation of daf-2, encoding an insulin receptor-like protein, alleviated the combined Ca/Cd toxicity on lifespan.
Wang
Dayong
D
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210009, China. dayongw@seu.edu.cn
Liu
Peidang
P
Yang
Yichao
Y
Shen
Lulu
L
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D002118:Calcium / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004785:Environmental Pollutants / Q000633:toxicity*; D006360:Heat-Shock Proteins / Q000235:genetics; D008136:Longevity / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests
D029742:Caenorhabditis elegans Proteins; D004785:Environmental Pollutants; D006360:Heat-Shock Proteins; C108431:hsp-16.2 protein, C elegans; D002104:Cadmium; D013482:Superoxide Dismutase; D002118:Calcium