Synthesis of CdTe/CdS/ZnS quantum dots and their application in imaging of hepatocellular carcinoma cells and immunoassay for alpha fetoprotein.
We report the imaging of hepatocellular carcinoma cells and the immunoassay for alpha fetoprotein (AFP) using CdTe/CdS/ZnS core-shell-shell QDs. Stable and high PLQY (20%-48%) CdTe/CdS/ZnS core-shell-shell QDs were synthesized by a stepwise process. Bioconjugation of the core-shell-shell QDs with streptavidin (SA) was successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HCC) cell line HepG2.2.15. Furthermore, the thioglycolic acid (TGA)-capped CdTe/CdS/ZnS core-shell-shell QDs fluorescence lifetime is longer than fluorescein, so it was first engaged to conjugate with antigen for the determination of protein (AFP) by fluorescence polarization immunoassay.
Tian
Jianniao
J
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, People's Republic of China. tianjn58@yahoo.com.cn
Liu
Rongjun
R
Zhao
Yanchun
Y
Peng
Yan
Y
Hong
Xue
X
Xu
Qing
Q
Zhao
Shulin
S
D019187:Cadmium Compounds / Q000138:chemical synthesis*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D003952:Diagnostic Imaging / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D008113:Liver Neoplasms / Q000175:diagnosis / Q000473:pathology; D008856:Microscopy, Fluorescence; D045663:Quantum Dots; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides / Q000138:chemical synthesis*; D013691:Tellurium; D017967:Zinc Compounds / Q000138:chemical synthesis*; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D019187:Cadmium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D000509:alpha-Fetoproteins; C034939:cadmium sulfide; D016650:Fluorescein-5-isothiocyanate; C031238:zinc sulfide; D013691:Tellurium; C028337:cadmium telluride