Diversity and evolution of Ty1-copia retroelements in representative tribes of Bambusoideae subfamily.
Ty1-copia retroelements have been found in all major plants and are largely responsible for the huge differences in the genome size. In this study we isolated and sequenced Ty1-copia reverse transcriptase (rt) gene fragments from 44 representative species of bamboo and nine cultivars or forms of Phyllostachys pubescens. Phylogenetic analysis of 72 distinct Ty1-copia rt sequences showed that Ty1-copia retroelements were widespread, diverse and abundant in these species of Bambusoideae subfamily. In addition, a molecular phylogeny of the species of the Bambusoideae subfamily was established by using the internal transcribed spacer sequences of nuclear ribosomal DNA (ITS) sequences. The comparison between ITS- and Ty1-copia rt- based trees is obviously incongruent. The results suggested either the existence of horizontal transfer events between phylogenetically distant species, or an ancestral Ty1-copia retroelement polymorphism followed by different evolution and stochastic losses.
Zhou
Ming-bing
MB
School of Agriculture and Biology, Shanghai Jiao Tong University, 200240 Shanghai, China.
Zhong
Hao
H
Zhang
Qin-hui
QH
Tang
Ke-Xuan
KX
Tang
Ding-Qin
DQ
D000595:Amino Acid Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D018626:Retroelements / Q000235:genetics*; D031787:Sasa / Q000201:enzymology / Q000235:genetics*; D013269:Stochastic Processes; D019895:Transposases / Q000235:genetics
D021903:DNA, Ribosomal Spacer; D018626:Retroelements; D019895:Transposases