Evaluation of the integrated database network system (IDNS) SmartGene software for analysis of 16S rRNA gene sequences for identification of Nocardia species.
16S rRNA gene sequences of 102 Nocardia isolates were analyzed using the Integrated Database Network System (IDNS) SmartGene centroid database. A total of 76% of the isolates were correctly identified. Discordant identifications were due to inadequate centroid length (3 species), inaccurate or insufficient entries in the public databases (5 species), and heterogeneous sequences among members of a species (1 species).
Conville
Patricia S
PS
Microbiology Service, Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. pconville@mail.nih.gov
Murray
Patrick R
PR
Zelazny
Adrian M
AM
D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D030541:Databases, Genetic; D003951:Diagnostic Errors / Q000706:statistics & numerical data; D006801:Humans; D009615:Nocardia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012984:Software
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S