Analysis of methylation patterns and expression profiles of p14ARF gene in patients with oral squamous cell carcinoma.
OBJECTIVE
To analyze the promoter methylation profile and mRNA expression of the p14ARF gene in oral squamous cell carcinoma (OSCC).


METHODS
Promoter methylation of the p14ARF gene was investigated by methylation-specific polymerase chain reaction in paraffin-embedded tissues from 76 patients with OSCC and 57 oral tissues used as healthy controls. Expression of p14ARF mRNA was also determined using real-time quantitative reverse-transcription PCR. The methylation status and mRNA level profile of the gene and their relationship with clinical data were analyzed.


RESULTS
Methylation of the p14ARF gene in OSCC was significantly increased compared to normal control tissues (chi(2) = 16.73, p < 0.0001). The relative expression of p14ARF mRNA in OSCC was not significantly different from that in healthy control samples.


CONCLUSIONS
Promoter methylation of p14ARF may be an important mechanism in OSCC, and its determination may be considered an important tool in the early diagnosis and treatment of OSCC.
Kordi-Tamandani
Dor Mohammad
DM
Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Moazeni-Roodi
Abdolkarim
A
Rigi Ladez
Mohammad Ayoub
MA
Hashemi
Mohammad
M
Birjandian
Elnaz
E
Torkamanzehi
Adam
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D019175:DNA Methylation; D055088:Early Detection of Cancer; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics* / Q000378:metabolism
D025803:Tumor Suppressor Protein p14ARF