title: Stromal CD10 and SPARC expression in ductal carcinoma in situ (DCIS) patients predicts disease recurrence.
abstract: The current classification of ductal carcinoma in situ (DCIS) is based on nuclear grade, architectural differentiation and the presence of necrosis that does not adequately predict the likelihood of recurrence after breast conserving therapy; therefore, there is a critical need to identify novel predictors of DCIS progression. Ninety seven cases of DCIS were included in the study. CD10 and SPARC expression in tumor stroma was assessed by standard immunoperoxidase method with ani-CD 10 and anti-SPARC antibodies. The staining was scored semi-quantitatively as negative, weak or strong. Statistical analysis was performed using the Fisher's exact test. Multivariable analysis was conducted utilizing Exact Logistic Regression software (SAS 9.1 and LogExact). A significant association was observed between the recurrence status and time to recurrence with expression of CD10 (p < 0.001) and SPARC (p < 0.001). When combining both SPARC and CD10 expression there was a strong correlation with the shortest time to recurrence. Stromal CD10 and SPARC expression are new markers of an increased risk for DCIS recurrence, independent of commonly assessed clinical parameters. Thus, stromal CD10 and SPARC expression levels are promising markers of DCIS recurrence and warrant evaluation in larger prospective studies.
authors.affiliation: Department of Pathology, Thomas Jefferson University, Philadelphia, PA, USA. agnieszka.witkiewicz@jefferson.edu
authors.forename: Agnieszka K
authors.initials: AK
authors.lastname: Witkiewicz
authors.forename: Boris
authors.initials: B
authors.lastname: Freydin
authors.forename: Inna
authors.initials: I
authors.lastname: Chervoneva
authors.forename: Magdalena
authors.initials: M
authors.lastname: Potoczek
authors.forename: Wendy
authors.initials: W
authors.lastname: Rizzo
authors.forename: Hallgeir
authors.initials: H
authors.lastname: Rui
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Brody
authors.forename: Gordon F
authors.initials: GF
authors.lastname: Schwartz
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lisanti
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D015260:Neprilysin / Q000378:metabolism*; D015676:Osteonectin / Q000378:metabolism*; D011379:Prognosis; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D015676:Osteonectin; D015260:Neprilysin