Suppressed CD31 expression in sarcoma-180 tumors after injection with Toxoplasma gondii lysate antigen in BALB/c mice.
The anti-tumorigenic effects of Toxoplasma gondii (RH) antigens were studied in a murine sarcoma-180 tumor model. To determine the anti-tumor effects, the reduction in tumor size and expression of CD31 (an angiogenesis marker in the tumor tissue) were examined after injection of BALB/c mice with T. gondii lysate antigen (TLA) or formalin-fixed, proliferation-inhibited, T. gondii tachyzoites. Tumors were successfully produced by an intradermal injection of sarcoma-180 cells with plain Matrigel in the mid-backs of mice. After injection with TLA or formalin-fixed T. gondii tachyzoites, the increase in tumor size and weight nearly stopped while tumor growth continued in control mice that were injected with PBS. CD31 expression in TLA-treated or formalin-fixed T. gondii-injected mice was lower than the control mice. Accordingly, the present study shows that the treatment of mice with formalin-fixed T. gondii or TLA in the murine sarcoma-180 tumor model results in a decrease of both tumor size and CD31 expression.
Pyo
Kyoung-Ho
KH
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, Korea.
Jung
Bong-Kwang
BK
Chai
Jong-Yil
JY
Shin
Eun-Hee
EH
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D012510:Sarcoma 180 / Q000473:pathology* / Q000628:therapy*; D014122:Toxoplasma / Q000737:chemistry*
D000953:Antigens, Protozoan; D000970:Antineoplastic Agents; D019408:Platelet Endothelial Cell Adhesion Molecule-1