title: Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China.
abstract: Cryptosporidium and Giardia infections are common causes of diarrhea worldwide. To better understand the transmission of human cryptosporidiosis and giardiasis in Henan, China, 10 Cryptosporidium-positive specimens and 18 Giardia-positive specimens were characterized at the species/genotype and subtype levels. Cryptosporidium specimens were analyzed by DNA sequencing of the small subunit rRNA and 60kDa glycoprotein genes. Among those genotyped, nine belonged to C. hominis and one C. felis, with the former belonging to three subtype families: Ia, Ib, and Id. The three Ib subtypes identified, IbA16G2, IbA19G2, and IbA20G2, were very different from the two common Ib subtypes (IbA9G3 and IbA10G2) found in other areas of the world. The distribution of Giardia duodenalis genotypes and subtypes was assessed by sequence analysis of the triosephosphate isomerase (tpi) gene. The assemblages A (eight belonging to A-I and four A-II) and B (belonging to six new subtypes) were found in 12 and six specimens, respectively. More systematic studies are needed to understand the transmission of Cryptosporidium and G. duodenalis in humans in China.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.forename: Xiaosan
authors.initials: X
authors.lastname: Zhang
authors.forename: Huili
authors.initials: H
authors.lastname: Zhu
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.forename: Changshen
authors.initials: C
authors.lastname: Ning
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Kanda
authors.initials: K
authors.lastname: Fu
authors.forename: Yaqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanru
authors.initials: Y
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002681:China; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D003967:Diarrhea / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D007223:Infant; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D016054:DNA, Protozoan; D012335:RNA, Ribosomal