title: In vitro evolution and analysis of HIV-1 LTR-specific recombinases.
abstract: Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)γc(-/-) mice.
authors.affiliation: Max-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Buchholz
authors.forename: Joachim
authors.initials: J
authors.lastname: Hauber
mesh_terms: D000818:Animals; D019020:Directed Molecular Evolution; D055786:Gene Knockout Techniques; D015316:Genetic Therapy; D057809:HEK293 Cells; D015658:HIV Infections / Q000235:genetics / Q000628:therapy*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D045522:Recombinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D045522:Recombinases