[Serum antibody response to different doses of hepatitis B vaccine made by recombinant DNA techniques in yeast and Hansenula polymorpha yeast].
OBJECTIVE
To observe the serum antibody response of the population, whose hepatitis B virus surface antigen(HBsAg), antibody to hepatitis B virus surface antigen (Anti-HBs), antibody to hepatitis B virus core antigen (Anti-HBc) are all negative to the different doses of Hepatitis B vaccine made by recombinant DNA techniques in Yeast and Hansenula Polymorpha Yeast.


METHODS
Collecting the blood of population vaccinated after 28 to 45 days, the serological method was used to detect Anti-HBs.


RESULTS
The Anti-HBs positive rate and GMC of 5 microg HepB-Y in the population <16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 97.23% and 199.26 mlU/ml, the Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 100.00% and 270.71 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference. The Anti-HBs positive rate and GMC of 10 microg HepB-Y in the population > or =16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 88.72% and 101.19 mlU/ml. Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 94.20% and 162.17 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference.


CONCLUSIONS
The population whose HBsAg, Anti-HBs, Anti-HBc negative vaccinated by HepB-Y, HepB-HPY can get better Anti-HBs response. The population younger than sixteen years old vaccinated by 10 microg HepB-HPY can get Anti-HBs positive rate and GMC higher than vaccinated by 5 microg HepB-Y. The Anti-HBs response of HepB-HPY is better than HepB-Y in the population > or =16 years old.
Sun
Jing
J
Liaoning Provincial Center for Disease Control and Prevention, Shenyang 110005, Liaoning, China.
Han
Yue
Y
Wang
Yan
Y
D000293:Adolescent; D000917:Antibody Formation / Q000276:immunology*; D004274:DNA, Recombinant / Q000235:genetics*; D004306:Dose-Response Relationship, Immunologic; D005818:Genetic Engineering / Q000379:methods*; D006511:Hepatitis B Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D010843:Pichia / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004274:DNA, Recombinant; D006511:Hepatitis B Antigens; D017325:Hepatitis B Vaccines