Prevalence and genetic diversity of adeno-associated viruses in bats from China.
Bats are increasingly being recognized as important natural reservoirs of different viruses. Adeno-associated viruses (AAVs) are widely distributed in primates and their distribution in bats is unknown. In this study, a total of 370 faecal swab samples from 19 bat species were collected from various provinces of China and examined for the presence of AAVs. The mean prevalence rate was 22.4% (83 positives out of 370 samples), ranging from 10 to 38.9% among different bat species. The genome sequence spanning the entire rep-cap ORFs was determined from one chosen AAV-positive sample (designated BtAAV-YNM). Phylogenetic analysis of the entire rep-cap ORF coding sequences suggested that BtAAV-YNM is relatively distant to known primate AAVs, but phylogenetically closer to porcine AAV strain Po3. Further analysis of the partial cap ORF sequences of bat AAV samples (n=49) revealed a remarkably large genetic diversity, with an average pairwise nucleotide identity of only 84.3%. Co-presence of multiple distinctive genotypes of bat AAV within an individual sample was also observed. These results demonstrated that diverse AAVs might be widely distributed in bat populations.
Li
Yan
Y
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, PR China.
Ge
Xingyi
X
Hon
Chung-Chau
CC
Zhang
Huajun
H
Zhou
Peng
P
Zhang
Yunzhi
Y
Wu
Yi
Y
Wang
Lin-Fa
LF
Shi
Zhengli
Z
D000818:Animals; D002681:China / Q000453:epidemiology; D002685:Chiroptera / Q000821:virology*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D000229:Dependovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins