title: Infrared imaging of an A549 cultured cell by a vibrational sum-frequency generation detected infrared super-resolution microscope.
abstract: We performed infrared (IR) spectroscopic imaging of molecular species in cultured cell interiors of A549 cells using in-house developed vibrational sum-frequency generation detected IR super-resolution microscope. The spatial resolution of this IR microscope was approximately 1.1 microm, which exceeds the diffraction limit of IR light. Therefore, we clearly observed differences in the signal intensity at various IR wavelengths which appear to originate from the differing IR absorptions of specific vibrational modes, and reveal the distribution of molecular species in the single cell. These results were never imaged with the conventional IR microscope.
authors.affiliation: Chemical Resources Laboratory, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kogure
authors.forename: Keiichi
authors.initials: K
authors.lastname: Inoue
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohmori
authors.forename: Miya
authors.initials: M
authors.lastname: Ishihara
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Fujii
authors.forename: Makoto
authors.initials: M
authors.lastname: Sakai
mesh_terms: D003585:Cell Biology / Q000295:instrumentation*; D045744:Cell Line, Tumor; D004579:Electron Transport; D004867:Equipment Design; D006801:Humans; D007259:Infrared Rays; D008175:Lung Neoplasms; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D012825:Silicon; D013059:Spectrum Analysis, Raman; D014732:Vibration
chemical_list: D012825:Silicon