Wide field-of-view microscope based on holographic focus grid illumination.
We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm x 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size--our demonstrated average FWHM spot diameter was 0.74 microm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.
Wu
Jigang
J
Department of Electrical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, California 91125, USA. jigang@caltech.edu
Cui
Xiquan
X
Zheng
Guoan
G
Wang
Ying Min
YM
Lee
Lap Man
LM
Yang
Changhuei
C
D006696:Holography / Q000295:instrumentation / Q000379:methods*; D008029:Lighting / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*