[Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells].
OBJECTIVE
To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.


METHODS
Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.


RESULTS
After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05).


CONCLUSIONS
Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.
Wu
Wei-dong
WD
Department of Surgery, Guangzhou Red-cross Hospital, Jinan Medical University, Guangzhou 510220, China. william0406@163.com
Fang
Chi-hua
CH
Yang
Zheng-xin
ZX
Bao
Jia-jin
JJ
D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D066246:ErbB Receptors; D006821:Hyaluronoglucosaminidase