Anti-wrinkle activity of Platycarya strobilacea extract and its application as a cosmeceutical ingredient.
In order to investigate the potential of Platycarya strobilacea fruit extract as an active ingredient for cosmetics, we measured their free-radical scavenging activity, elastase inhibitory activity, the expression of MMP-1 (matrix metalloproteinase-1), and type I collagen synthesis in normal human fibroblast cells. To isolate the main component compounds from P. strobilacea fruit extract, we purified the extract through solvent fractionation, column chromatography, and recrystallization. The component compounds were identified as ellagic acid and 4-O-xyloside of ellagic acid (ellagic acid 4-O-xylopyranoside). P. strobilacea fruit extract and ellagic acid increased the expression of type I collagen mRNA in a dose-dependent manner (up to 37% and 41% at 20 microg/ml and 1.0 microg/ml, respectively), comparable to that of ascorbic acid (up to 39% at 500 muM). A clinical study of measurements using visual evaluation and image analysis showed a statistically significant difference (p < 0.05) between the effects of the test and placebo products. This result suggests that P. strobilacea fruit extract could be used as an active ingredient for antiaging cosmetics.
Kim
Young Heui
YH
R&D Center, Bioland Ltd., Cheonan, Chungnam, Republic of Korea.
Kim
Ki Ho
KH
Han
Chang Sung
CS
Yang
Hong Chul
HC
Park
Sun Hee
SH
Jang
Hye-In
HI
Kim
Jin-Wan
JW
Choi
Yun-Sun
YS
Lee
Nam Ho
NH
D000328:Adult; D001713:Biphenyl Compounds / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D003358:Cosmetics / Q000737:chemistry / Q000494:pharmacology*; D004311:Double-Blind Method; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D029525:Juglandaceae / Q000737:chemistry*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015595:Skin Aging / Q000187:drug effects*
D001713:Biphenyl Compounds; D024042:Collagen Type I; D003358:Cosmetics; D016166:Free Radical Scavengers; D061965:Matrix Metalloproteinase Inhibitors; D010851:Picrates; D010936:Plant Extracts; D012333:RNA, Messenger; C004931:1,1-diphenyl-2-picrylhydrazyl; D010196:Pancreatic Elastase; D020781:Matrix Metalloproteinase 1