title: Inhibition of 4-NQO-induced F433 rat tongue carcinogenesis by oleuropein-rich extract.
abstract: Olive leaf extract provides nutritional support for detoxification at the cellular level, when the body is under stress. The present study aimed to evaluate the chemopreventive effect of oleuropein-rich extract (ORE) on 4-NQO-induced rat tongue carcinogenesis. Eighty male F344 rats, 6 weeks age were divided into 5 groups (10 animals each for groups 1 and 2 and 20 each for groups 3, 4, and 5). Group 1 served as an untreated control. Group 2 was given ORE-containing diet alone. Rats of groups 3, 4, and 5 were given daily 20 ppm 4-NQO in drinking water for 8 weeks. Group 4 was fed diets containing ORE, concomitantly with the time of carcinogen exposure and continued 1 week after its stoppage. Group 5 was fed diets mixed with ORE starting 1 week after cessation of 4-NQO treatment. The experiment was terminated when the rats aged 37 weeks, and all animals were euthanized. The tongues were carefully inspected for pathological lesions, excised, and were processed for c-Met and Ki-67 immunohistochemical examination. The gross inspection, histopathological and immunohistochemical results of the present study showed a beneficial regression effect of ORE on tumor progression, especially when it was administered concomitantly with 4-NQO rather than when given after the stoppage of the carcinogenic material. In conclusion, ORE has a chemopreventive role in tongue squamous cell carcinoma, and further studies are needed to explore the molecular mechanisms of its tumor suppressive effect at this level.
authors.affiliation: Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. Grawish2005@yahoo.com
authors.forename: Mohammed E
authors.initials: ME
authors.lastname: Grawish
authors.forename: Manal M
authors.initials: MM
authors.lastname: Zyada
authors.forename: Ahmed R
authors.initials: AR
authors.lastname: Zaher
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity; D007150:Immunohistochemistry; D057888:Iridoid Glucosides; D039823:Iridoids; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D031658:Olea / Q000737:chemistry; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D011714:Pyrans / Q000494:pharmacology*; D015363:Quinolones / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D014062:Tongue Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*
chemical_list: C120195:4-nitroquinolone-1-oxide; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D002273:Carcinogens; D057888:Iridoid Glucosides; D039823:Iridoids; D019394:Ki-67 Antigen; D010936:Plant Extracts; D011714:Pyrans; D015363:Quinolones; C002769:oleuropein; D015112:4-Nitroquinoline-1-oxide; D019859:Proto-Oncogene Proteins c-met