Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila.
Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila.
Tsuda
Manabu
M
Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan.
Ootaka
Ryousuke
R
Ohkura
Chiaki
C
Kishita
Yoshihito
Y
Seong
Ki-Hyeon
KH
Matsuo
Takashi
T
Aigaki
Toshiro
T
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics; D008136:Longevity / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010269:Paraquat / Q000633:toxicity; D010641:Phenotype; D050050:Protein Carbonylation / Q000187:drug effects; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; D016877:Oxidants; C516042:Trx-2 protein, Drosophila; D013879:Thioredoxins; D010269:Paraquat