A comparative study of microfocus CT and histomorphometry in the evaluation of bone augmentation in rat calvarium.
Microfocus computed tomography (micro-CT; R_mCT) is a dynamic noninvasive method for measuring bone regeneration. This study evaluated whether R_mCT was equivalent to histomorphometry in assessing bone augmentation. Two plastic caps of graft material with (experiment) or without hydroxyapatite (HA; control) were placed in the exposed calvaria of rats. Images of bone augmentation within the plastic caps were then taken using R_mCT. Histological sections were cut along the same plane as that used for the micro-CT images. Bone regeneration beyond the skeletal envelope occurred at both the experimental and control sites. Bone volume also increased at both sites. In addition, consistent patterns of bone formation were observed in both R_mCT and histological images. R_mCT analysis enables highly quantitative and qualitative measurement of bone augmentation in living animals.
Kochi
Go
G
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Sato
Shuichi
S
Ebihara
Hajime
H
Hirano
Jiro
J
Arai
Yoshinori
Y
Ito
Koichi
K
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000627:therapeutic use; D002113:Calcification, Physiologic / Q000502:physiology; D017886:Durapatite / Q000627:therapeutic use; D048091:Guided Tissue Regeneration / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008297:Male; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D055114:X-Ray Microtomography
D001672:Biocompatible Materials; D018786:Bone Substitutes; D017886:Durapatite