Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay.
A surface plasmon resonance (SPR) immunoassay using a monoclonal antibody was developed to measure nivalenol (NIV) and deoxynivalenol (DON) contamination in wheat. A highly sensitive and stable DON-immobilized sensor chip was prepared, and an SPR detection procedure was developed. The competitive inhibition assay used a monoclonal antibody that cross-reacts with NIV and DON. The half maximal inhibitory concentration (IC(50)) values of the SPR assay were 28.8 and 14.9 ng mL(-1) for NIV and DON, respectively. The combined responses of NIV and DON in wheat were obtained using a simultaneous detection assay in a one-step cleanup procedure. NIV and DON were separated using a commercial DON-specific immunoaffinity column (IAC) and their responses were obtained using an independent detection assay. Spiked tests using these toxins revealed that recoveries were in the range 91.5-107% with good relative standard deviations (RSDs) (0.40-4.1%) and that detection limits were 0.1 and 0.05 mg kg(-1) for NIV and DON, respectively. The independent detection using IAC showed detection limits of 0.2 and 0.1 mg kg(-1) for NIV and DON, respectively. SPR analysis results were correlated with those obtained using a conventional LC/MS/MS method for wheat co-contaminated with NIV and DON. These results suggested that the developed SPR assay is a practical method to rapidly screen the NIV and DON co-contamination of wheat and one of a very few immunoassays to detect NIV directly.
Kadota
Tomoyuki
T
Central Laboratories for Frontier Technology, Center for Food Safety Science, Kirin Holdings Company, Limited, 3 Miyahara, Takasaki, Gunma 370-1295, Japan. Tomoyuki Kadota@kirin.co.jp
Takezawa
Yoko
Y
Hirano
Satoshi
S
Tajima
Osamu
O
Maragos
Chris M
CM
Nakajima
Takashi
T
Tanaka
Toshitsugu
T
Kamata
Yoichi
Y
Sugita-Konishi
Yoshiko
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D007118:Immunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry
D000911:Antibodies, Monoclonal; D014255:Trichothecenes; D012710:Serum Albumin, Bovine; C038405:nivalenol; C007262:deoxynivalenol