Optimization of poly-epsilon-lysine production by Streptomyces noursei NRRL 5126.
Poly-epsilon-lysine (epsilon-PL) is a non-toxic biopolymer with antimicrobial properties. The production of epsilon-PL by Streptomyces noursei NRRL 5126 shake-flask culture was optimized by identifying the most significant medium components which affect epsilon-PL production (glycerol, proteose peptone and ammonium sulphate) by Placket-Burman design and by application of an evolutionary operation (EVOP) to determine the optimal concentrations of these components. The epsilon-PL yield increased from 41.81 g/l in basal medium to 98.07 g/l in the EVOP-optimized medium containing 3% glycerol, 1% proteose peptone and 0.8% ammonium sulphate. Further improvements in media composition and culture conditions will be required to obtain yields comparable to those obtained with current commercial strains such as Streptomyces albulus.
Bankar
Sandip B
SB
Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga (E), Mumbai 400 019, India. sandipbankar@gmail.com
Singhal
Rekha S
RS
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008954:Models, Biological; D009584:Nitrogen / Q000494:pharmacology; D011107:Polylysine / Q000096:biosynthesis*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D011107:Polylysine; D002244:Carbon; D009584:Nitrogen