title: Wide field-of-view microscope based on holographic focus grid illumination.
abstract: We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm x 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size--our demonstrated average FWHM spot diameter was 0.74 microm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.
authors.affiliation: Department of Electrical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, California 91125, USA. jigang@caltech.edu
authors.forename: Jigang
authors.initials: J
authors.lastname: Wu
authors.forename: Xiquan
authors.initials: X
authors.lastname: Cui
authors.forename: Guoan
authors.initials: G
authors.lastname: Zheng
authors.forename: Ying Min
authors.initials: YM
authors.lastname: Wang
authors.forename: Lap Man
authors.initials: LM
authors.lastname: Lee
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D006696:Holography / Q000295:instrumentation / Q000379:methods*; D008029:Lighting / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*