Expression of epitope-tagged proteins in plants.
Although immunoelectron microscopy is a powerful tool for visualizing the subcellular localization of target proteins, it is difficult to obtain and purify the specific antibodies required for this method. Instead of raising antibodies against individual target proteins, the use of transgenic plants expressing epitope-tagged proteins and commercially available antibodies simplifies the subcellular localization of target proteins. In this chapter, an improved method for producing transgenic plants that express epitope-tagged proteins and can be used for immunoelectron microscopic analysis is described.
Furuichi
Takuya
T
Research Institute for Bioresources, Okayama University, Okayama, Japan.
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D000939:Epitopes / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D011993:Recombinant Fusion Proteins