title: Generation of Fgfr3 conditional knockout mice.
abstract: Fibroblast growth factor receptor 3 (FGFR3), highly conserved in both humans and murine, is one of key tyrosine kinase receptors for FGF. FGFR3 is expressed in different tissues, including cartilage, brain, kidney, and intestine at different development stages. Conventional knockout of Fgfr3 alleles leads to short life span, and overgrowth of bone. In clinic, human FGFR3 mutations are responsible for three different types of chondrodysplasia syndromes including achondroplasia (ACH), hypochondroplasia (HCH) and thanatophoric dysplasia (TD). For better understanding of the roles of FGFR3 in different tissues at different stages of development and in pathological conditions, we generated Fgfr3 conditional knockout mice in which loxp sites flank exons 9-10 in the Fgfr3 allele. We also demonstrated that Cre-mediated recombination using Col2a1-Cre, a Cre line expressed in chondrocyte during bone development, results in specific deletion of the gene in tissues containing cartilage. This animal model will be useful to study distinct roles of FGFR3 in different tissues at different ages.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Center of Bone Metabolism and Repair, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Su
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xu
authors.forename: Cuiling
authors.initials: C
authors.lastname: Li
authors.forename: Qifen
authors.initials: Q
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Can
authors.initials: C
authors.lastname: Li
authors.forename: Siyu
authors.initials: S
authors.lastname: Chen
authors.forename: Fengtao
authors.initials: F
authors.lastname: Luo
authors.forename: Lingxian
authors.initials: L
authors.lastname: Yi
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Du
authors.forename: Haiyang
authors.initials: H
authors.lastname: Huang
authors.forename: Chuxia
authors.initials: C
authors.lastname: Deng
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D018274:Electroporation; D005838:Genotype; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051498:Receptor, Fibroblast Growth Factor, Type 3