title: Taurine supplementation: involvement of cholinergic/phospholipase C and protein kinase A pathways in potentiation of insulin secretion and Ca2+ handling in mouse pancreatic islets.
abstract: Taurine (TAU) supplementation increases insulin secretion in response to high glucose concentrations in rodent islets. This effect is probably due to an increase in Ca2+ handling by the islet cells. Here, we investigated the possible involvement of the cholinergic/phospholipase C (PLC) and protein kinase (PK) A pathways in this process. Adult mice were fed with 2% TAU in drinking water for 30 d. The mice were killed and pancreatic islets isolated by the collagenase method. Islets from TAU-supplemented mice showed higher insulin secretion in the presence of 8.3 mm-glucose, 100 μm-carbachol (Cch) and 1 mm-3-isobutyl-1-methyl-xanthine (IBMX), respectively. The increase in insulin secretion in response to Cch in TAU islets was accompanied by a higher intracellular Ca2+ mobilisation and PLCβ2 protein expression. The Ca2+ uptake was higher in TAU islets in the presence of 8.3 mm-glucose, but similar when the islets were challenged by glucose plus IBMX. TAU islets also showed an increase in the expression of PKAα protein. This protein may play a role in cation accumulation, since the amount of Ca2+ in these islets was significantly reduced by the PKA inhibitors: N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide (H89) and PK inhibitor-(6-22)-amide (PKI). In conclusion, TAU supplementation increases insulin secretion in response to glucose, favouring both influx and internal mobilisation of Ca2+, and these effects seem to involve the activation of both PLC-inositol-1,4,5-trisphosphate and cAMP-PKA pathways.
authors.affiliation: Departamento de Anatomia, Biologia Celular e Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil. rosaneribeirobio@yahoo.com.br
authors.forename: Rosane A
authors.initials: RA
authors.lastname: Ribeiro
authors.forename: Emerielle C
authors.initials: EC
authors.lastname: Vanzela
authors.forename: Camila A M
authors.initials: CA
authors.lastname: Oliveira
authors.forename: Maria L
authors.initials: ML
authors.lastname: Bonfleur
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Boschero
authors.forename: Everardo M
authors.initials: EM
authors.lastname: Carneiro
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm; D019587:Dietary Supplements; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D010703:Phorbol Esters / Q000494:pharmacology; D054799:Phospholipase C beta / Q000235:genetics / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism; D013654:Taurine / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D007328:Insulin; D010703:Phorbol Esters; D013654:Taurine; D005576:Colforsin; C008874:phorbol-12,13-diacetate; D002217:Carbachol; D017868:Cyclic AMP-Dependent Protein Kinases; D051571:Protein Kinase C-alpha; D054799:Phospholipase C beta; C518037:Plcb2 protein, mouse; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine