title: Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering.
abstract: To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.
authors.affiliation: Department of Burn, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. hanchunmao1@126.com
authors.forename: Chun-mao
authors.initials: CM
authors.lastname: Han
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Jin-zhang
authors.initials: JZ
authors.lastname: Sun
authors.forename: Hai-fei
authors.initials: HF
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Chang-you
authors.initials: CY
authors.lastname: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan; D024042:Collagen Type I; D015718:Fibrin Tissue Adhesive; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D012867:Skin / Q000166:cytology / Q000254:growth & development; D014014:Tissue Adhesives; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D048271:Chitosan