title: [Generation of transgenic animals, expressing alpha- and beta-chains of autoreactive TCR].
abstract: Transgenic animal studies has become a key approach for gene function analysis as well as for modeling of different human diseases, including autoimmune diseases caused by activation of T-lymphocyte clones whose TCRs possesses high affinity for syngeneic MHC molecules. In this study we cloned genes, encoding alpha- and beta- chains of autoreactive TCR of hybridoma 7, specific for syngeneic MHC class II molecules A(b). Amplified DNA fragments, containing rearranged genomic DNA of alpha- and beta-chains of hybridoma 7 were cloned into special cassette vectors, containing natural promoter and enhancer elements for direct expression of genes encoding TCR alpha- and beta-chains in T-lymphocytes of transgenic animals. Using this cassette vectors we generated animals in which most of peripheral T-lymphocytes carry alpha-chain, as well as animals with expression of beta-chain transgene of autoreactive TCR. Obtained animals may serve to explain a number of intrathymic selection processing features and T cell maturation as well as to serve as experimental models for development of new approaches to therapy of autoimmune diseases.
authors.forename: E S
authors.initials: ES
authors.lastname: Zvezdova
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Silaeva
authors.forename: M S
authors.initials: MS
authors.lastname: Vagida
authors.forename: E V
authors.initials: EV
authors.lastname: Mariukhnich
authors.forename: A V
authors.initials: AV
authors.lastname: Deĭkin
authors.forename: T G
authors.initials: TG
authors.lastname: Ermolkevich
authors.forename: S G
authors.initials: SG
authors.lastname: Kadulin
authors.forename: E R
authors.initials: ER
authors.lastname: Sadchikova
authors.forename: I L
authors.initials: IL
authors.lastname: Gol'dman
authors.forename: D B
authors.initials: DB
authors.lastname: Kazanskiĭ
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D015870:Gene Expression; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008822:Mice, Transgenic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics / Q000276:immunology*
chemical_list: D006649:Histocompatibility Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta