Paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Begonia semperflorens.
A nitrogen-fixing bacterium, designated strain Be17(T), was isolated from rhizosphere soil of Begonia semperflorens planted in Beijing Botanical Garden, PR China. Phylogenetic analyses based on a segment of the nifH gene sequence and a full-length 16S rRNA gene sequence revealed that strain Be17(T) was a member of the genus Paenibacillus. High levels of 16S rRNA gene sequence similarity were found between strain Be17(T) and Paenibacillus graminis RSA19(T) (97.9 %), Paenibacillus sonchi LMG 24727(T) (97.8 %), Paenibacillus riograndensis CECT 7330(T) (96.2 %) and Paenibacillus borealis DSM 13188(T) (96.1 %), respectively. Levels of 16S rRNA gene sequence similarity between strain Be17(T) and the type strains of other recognized members of the genus Paenibacillus were below 96.0 %. However, the DNA-DNA hybridization values between strain Be17(T) and P. graminis RSA19(T), P. sonchi LMG 24727(T) and P. riograndensis CECT 7330(T) were 47.9 %, 38.7 % and 37.5 %, respectively. The DNA G+C content of strain Be17(T) was 52.9 mol%. The major fatty acid component of strain Be17(T) was anteiso-branched C(15 : 0) (30.92 %). The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of its phenotypic characteristics, 16S rRNA gene sequences, DNA G+C content, DNA-DNA relatedness, chemotaxonomic properties and nifH gene sequence, strain Be17(T) represents a nitrogen-fixing strain of a novel species of the genus Paenibacillus, for which the name Paenibacillus jilunlii sp. nov. is proposed. The type strain is Be17(T) ( = CGMCC 1.10239(T) = DSM 23019(T)).
Jin
Hao-Jie
HJ
State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Zhou
Yu-Guang
YG
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences Datun Road, Chaoyang District, Beijing 100101, PR China.
Liu
Hong-Can
HC
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences Datun Road, Chaoyang District, Beijing 100101, PR China.
Chen
San-Feng
SF
State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D031112:Begoniaceae / Q000382:microbiology*; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics; D056507:Paenibacillus / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000596:Amino Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase