title: Peritoneal transforming growth factor beta-1 expression during prolonged laparoscopic procedures.
abstract: BACKGROUND
  Laparoscopic surgery may affect peritoneal physiology. Short-term laparoscopic surgery does not affect peritoneal transforming growth factor beta (TGF-b1) expression. The current study was conducted to evaluate the hypothesis that prolonged laparoscopic surgery may affect peritoneal TGF-b1 expression.
  
  
  METHODS
  In the first study, 24 patients scheduled for a right colonic resection were enrolled in the trial. Twelve underwent conventional surgery (CCR) and 12 were operated on laparoscopically (LCR). In the second study, 12 patients undergoing laparoscopic gastric bypass (LGB) surgery for morbid obesity were included. Biopsies of the parietal peritoneum were taken at standardized moments during the procedures. Tissue concentrations of active and total TGF-b1 were measured by using enzyme-linked immunosorbent assays.
  
  
  RESULTS
  During the LCR, there was a significant increase in peritoneal active TGF-b1 levels (P < 0.05). A similar, but not significant, trend was observed during the CCR. A similar pattern was seen in the total TGF-b1 concentrations during both procedures. The LGB procedure did not affect peritoneal active or total TGF-b1 concentrations. During the procedure, both the active and total TGF-b1 levels were significantly higher in the LCR, when compared to the LGB, group (P < 0.05).
  
  
  CONCLUSIONS
  Prolonged laparoscopic surgery may affect peritoneal TGF-b1 expression, depending on the procedure performed. Considering the role of TGF-b1 in various biologic processes, including adhesiogenesis and oncology, these results may have clinical consequences.
authors.affiliation: Department of Surgery, Alysis Zorggroep, Arnhem, The Netherlands.
authors.forename: Mare M A
authors.initials: MM
authors.lastname: Lensvelt
authors.forename: Walter J A
authors.initials: WJ
authors.lastname: Brokelman
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Ivarsson
authors.forename: Peter
authors.initials: P
authors.lastname: Falk
authors.forename: Michel M P J
authors.initials: MM
authors.lastname: Reijnen
mesh_terms: D000328:Adult; D003082:Colectomy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D010535:Laparoscopy; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000601:surgery; D010537:Peritoneum / Q000737:chemistry*; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000032:analysis*
chemical_list: D053773:Transforming Growth Factor beta1