[Investigation on the process of sapindus saponin purified with macroporous adsorption resin and screening of its bacteriostasis].
OBJECTIVE
To study the technological parameters of the purification process of saponins with macroporous adsorption resin.


METHODS
The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers. Bacteriostasis activity of each parts eluted was evaluated by the mean of cup-plate method.


RESULTS
13.6 mL of the extraction of sapindus saponin (crude drugs 0.01 g/mL) was purified with a column of macroporous adsorption resin (phi15 mm x H90 mm, dry weight 2.5 g) and washed with 3BV of distilled water, then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol, most of saponins were collected in the 70% ethanol.


CONCLUSIONS
With macroporous adsorption resin adsorbing and purifying, the elutive ratio of saponins was 93.8% and the purity reached 250.1%. So this process of applying macroporous adsorption resin to adsorb and purify saponins is feasible, and supplies reference to the purification of other types of saponin.
Fu
Yong
Y
Chengdu University of TCM, Chengdu 610075, China.
Lei
Peng
P
Han
Yu-mei
YM
Yan
Dan
D
D000327:Adsorption; D002176:Candida albicans / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry* / Q000145:classification; D032127:Sapindus / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water
D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; D012503:Saponins; D014867:Water; D000431:Ethanol