title: Staging of alpha-synuclein in the olfactory bulb in a model of Parkinson's disease: cell types involved.
abstract: Impaired olfaction is an early symptom of Parkinson's disease. The underlying neuropathology likely includes alpha-synucleinopathy in the olfactory bulb at an earlier stage (Braak's stage1) than pathology in the substantia nigra, which is not observed until stage 3. In this report, we investigated the distribution and cell types affected by alpha-synuclein in the olfactory bulb of transgenic mice (2-8 months of age) expressing the human A53T variant of alpha-synuclein. alpha-Synuclein immunostaining progressively affects interneurons and mitral cells. Double labeling studies demonstrate that dopaminergic cells are hardly involved, whereas glutamatergic- and calcium binding protein-positive cells are severely affected. This temporal evolution and the cell types expressing alpha-synuclein are reminiscent of idiopathic Parkinson's disease and support the usefulness of this model to address specific topics in the premotor phase of the disease.
authors.affiliation: Laboratorio de Neuroanatomía Humana, Departamento de Ciencias Médicas, Facultad de Medicina, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ubeda-Bañon
authors.forename: Daniel
authors.initials: D
authors.lastname: Saiz-Sanchez
authors.forename: Carlos
authors.initials: C
authors.lastname: de la Rosa-Prieto
authors.forename: Alicia
authors.initials: A
authors.lastname: Mohedano-Moriano
authors.forename: Noelia
authors.initials: N
authors.lastname: Fradejas
authors.forename: Soledad
authors.initials: S
authors.lastname: Calvo
authors.forename: Lucia
authors.initials: L
authors.lastname: Argandoña-Palacios
authors.forename: Susana
authors.initials: S
authors.lastname: Garcia-Muñozguren
authors.forename: Alino
authors.initials: A
authors.lastname: Martinez-Marcos
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000145:classification / Q000378:metabolism*; D009830:Olfactory Bulb / Q000378:metabolism* / Q000473:pathology*; D010300:Parkinson Disease / Q000235:genetics / Q000473:pathology*; D010320:Parvalbumins / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D010320:Parvalbumins; D014452:Ubiquitins; D051844:alpha-Synuclein; D014446:Tyrosine 3-Monooxygenase