title: Biosorption of uranium by Saccharomyces cerevisiae and surface interactions under culture conditions.
abstract: Few studies have focused on biosorption by microorganisms under culture conditions. To explore the biosorption of uranium by Saccharomyces cerevisiae under culture conditions, the S. cerevisiae growth curve, biosorption capacity and surface interaction under batch culture conditions were investigated in this study. The growth curve showed that uranium (<300mgL(-1)) did not markedly inhibit the growth of S. cerevisiae under short culture time. The maximum scavenging efficiency reached 92.4% under 6-10h culture conditions, and the adsorption quantity of S. cerevisiae increased with initial uranium concentration. Centrifuging and drying after biosorption caused the volume reduction ratio to reach 99%. Scanning electron microscope results demonstrated that uranium interacted with yeast cell surfaces, as well as culture medium, and produced uranium precipitate on cell surfaces. Fourier transformed infrared spectra revealed that cell walls were the major sorption sites, and -O--H, -C==O and -PO(2-) contributed to the major binding groups.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China. dragonlmx@126.com
authors.forename: Mingxue
authors.initials: M
authors.lastname: Liu
authors.forename: Faqin
authors.initials: F
authors.lastname: Dong
authors.forename: Xiuying
authors.initials: X
authors.lastname: Yan
authors.forename: Wenming
authors.initials: W
authors.lastname: Zeng
authors.forename: Liangyu
authors.initials: L
authors.lastname: Hou
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Pang
mesh_terms: D000327:Adsorption; D018929:Cell Culture Techniques / Q000379:methods*; D011850:Radioactive Waste / Q000517:prevention & control*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism*; D014501:Uranium / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011850:Radioactive Waste; D014501:Uranium