title: Liver sinusoidal endothelium: a microenvironment-dependent differentiation program in rat including the novel junctional protein liver endothelial differentiation-associated protein-1.
abstract: Liver sinusoidal endothelium (LSEC) is a prime example of organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program in the rat we used a two-sided gene expression profiling approach comparing LSEC freshly isolated ex vivo with both lung microvascular endothelial cells (LMEC) and with LSEC cultured for 42 hours. The LSEC signature consisted of 48 genes both down-regulated in LMEC and in LSEC upon culture (fold change >7 in at least one comparison); quantitative reverse-transcription polymerase chain reaction confirmation of these genes included numerous family members and signaling pathway-associated molecules. The LSEC differentiation program comprised distinct sets of growth (Wnt2, Fzd4, 5, 9, Wls, vascular endothelial growth factors [VEGFR] 1, 2, 3, Nrp2) and transcription factors (Gata4, Lmo3, Tcfec, Maf) as well as endocytosis-related (Stabilin-1/2, Lyve1, and Ehd3) and cytoskeleton-associated molecules (Rnd3/RhoE). Specific gene induction in cultured LSEC versus freshly isolated LSEC as well as LMEC (Esm-1, Aatf) and up-regulation of gene expression to LMEC levels (CXCR4, Apelin) confirmed true transdifferentiation of LSEC in vitro. In addition, our analysis identified a novel 26-kDa single-pass transmembrane protein, liver endothelial differentiation-associated protein (Leda)-1, that was selectively expressed in all liver endothelial cells and preferentially localized to the abluminal cell surface. Upon forced overexpression in MDCK cells, Leda-1 was sorted basolaterally to E-cadherin-positive adherens junctions, suggesting functional involvement in cell adhesion and polarity.
  
  
  CONCLUSIONS
  Comparative microvascular analysis in rat identified a hepatic microenvironment-dependent LSEC-specific differentiation program including the novel junctional molecule Leda-1.
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Medical Center and Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany. cyrill.geraud@umm.de
authors.forename: Cyrill
authors.initials: C
authors.lastname: Géraud
authors.forename: Kai
authors.initials: K
authors.lastname: Schledzewski
authors.forename: Alexandra
authors.initials: A
authors.lastname: Demory
authors.forename: Diana
authors.initials: D
authors.lastname: Klein
authors.forename: Miriam
authors.initials: M
authors.lastname: Kaus
authors.forename: Francis
authors.initials: F
authors.lastname: Peyre
authors.forename: Carsten
authors.initials: C
authors.lastname: Sticht
authors.forename: Konstantin
authors.initials: K
authors.lastname: Evdokimov
authors.forename: Shun
authors.initials: S
authors.lastname: Lu
authors.forename: Astrid
authors.initials: A
authors.lastname: Schmieder
authors.forename: Sergij
authors.initials: S
authors.lastname: Goerdt
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D002196:Capillaries / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D004285:Dogs; D004705:Endocytosis / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005819:Genetic Markers; D008099:Liver / Q000098:blood supply*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D040262:Receptors, Vascular Endothelial Growth Factor / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D005819:Genetic Markers; D008565:Membrane Proteins; C550858:Pianp protein, rat; D014157:Transcription Factors; D040262:Receptors, Vascular Endothelial Growth Factor