[Inhibitory effect of photodynamic therapy combined with paclitaxel on the proliferation of esophageal carcinoma Eca-109 cells].
OBJECTIVE
To investigate the inhibitory effect of photodynamic therapy (PDT) in combination with paclitaxel (PCT) on proliferation in esophageal carcinoma Eca-109 cells line.


METHODS
Eca-109 cells were treated with PCT alone, HPD alone at different doses, or their combinations. For the combined treatments, the cells were exposed to PCT for 12 h followed by incubation with HPD at high, middle or low concentrations for 4 h. PDT was then performed on these treated cells and fluorescence microscopic observation was made before and after PDT. The cell survival was measured by MTT assay, and the cell apoptosis rate analyzed by flow cytometry after a 24-h cell incubation following PDT.


RESULTS
The fluorescence excitation of the cells was weakened after PDT. Combined treatments resulted in significantly lowered cell survival rate and increased cell apoptosis rates as compared to those of the control cells and the cells treated with PCT alone and low-dose HPD (P<0.01). Significant differences were also noted among the cells exposed to HPD at different concentrations (P<0.05).


CONCLUSIONS
PDT combined with PCT have significant synergetic effects in inhibiting the proliferation of human esophageal carcinoma cells and inducing their apoptosis in vitro.
Zhang
Zhi-feng
ZF
Department of Chemotherapy, First Affiliated Hospital of Fujian Medical University, Fuzhou 35005, China. zzfhl2004@126.com
Huang
Yi
Y
Shi
Xi
X
Wang
Ri-xiong
RX
Lin
Xiao-yan
XY
Lin
Xue-de
XD
Zheng
Hang
H
Li
Li-bo
LB
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D017239:Paclitaxel / Q000494:pharmacology*; D010778:Photochemotherapy
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel