Placental-derived mesenchyme influences chorionic gonadotropin and progesterone secretion of human embryonic stem cell-derived trophoblasts.
Studies of early placental development in humans are difficult because of limitations on experimental material availability from the perimplantation period. We used a coculture system to determine the effects of various effector cell types on trophoblast differentiation. Enhanced green fluorescent protein (EGFP)-expressing H1 human embryonic stem cells were used in co-suspension with human term placental fibroblasts (TPFs) and dermal fibroblasts (CI2F) to form combination embryoid bodies (EBs), with the goal of recapitulating placental morphogenesis through incorporation of placental mesenchymal cells. Overall, the results demonstrated that when using mesenchymal cells for EB preparation from term placentas (TPF), combination EB-derived trophoblasts secrete higher levels of human chorionic gonadotropin (hCG) and progesterone compared to EBs made without effector cells, whereas there was no effect of dermal fibroblasts. This is due to the secretory activity of the EB-derived trophoblasts and not due to the number of differentiated trophoblasts per EB, demonstrating that nontrophoblast cells of the placenta can influence trophoblast endocrine activity.
Giakoumopoulos
Maria
M
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA.
Siegfried
Leah M
LM
Dambaeva
Svetlana V
SV
Garthwaite
Mark A
MA
Glennon
M Clay
MC
Golos
Thaddeus G
TG
D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D014327:Trophoblasts / Q000378:metabolism*
D006063:Chorionic Gonadotropin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011374:Progesterone