Recombinant mid gut antigen (Bm95) as a vaccine against Indian Rhiphicephalus haemaphysaloides in Bos indicus cattle.
In the present study, we report for the first time the efficacy of recombinant Bm95 mid gut antigen isolated from an Argentinean strain of Rhipicephalus microplus strain A in controlling the tick infestations in India. The synthetic gene for Bm95 optimized for expression in yeast was obtained and used to generate yeast transformants expressing Bm95 which was purified to apparent homogeneity. Liquid chromatography-mass spectrometry analysis of the purified protein confirmed its identity as Bm95. Vaccine was prepared by blending various concentrations of purified Bm95 with aluminium hydroxide as an adjuvant. Immunogenicity studies of the vaccine in rabbits and cattle indicated that the vaccine was highly immunogenic. The efficacy studies of the vaccine was done in cattle. Naïve Bos indicus cattle were vaccinated with the recombinant vaccine and were challenged with the larval, nymphal and adult forms of Rhiphicephalus haemaphysaloides. The vaccine protected the animals from larval, nymph and adult tick challenges with an efficacy of 98.7%, 84.6% and 78.9% respectively. The results obtained from the above studies clearly demonstrated the advantage and possibilities of the use of Bm95 in controlling R. haemaphysaloides infestations in the field.
Sugumar
Parthasarathy
P
Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad, India.
Chandran
Dev
D
Sudha Rani
Gudavalli
G
Shahana
Pallichera Vijayan
PV
Maske
Dasarath Keshavrao
DK
Rangarajan
Pundi Narasimhan
PN
Mangamoori
Lakshmi Narasu
LN
Srinivasan
Villuppanoor Alwar
VA
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D003001:Cloning, Molecular; D005260:Female; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology*; D048494:Rhipicephalus / Q000276:immunology*; D013984:Tick Infestations / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*
D000941:Antigens; D011994:Recombinant Proteins; D014612:Vaccines