title: Risk factors in malignant transformation of gastric epithelial neoplasia categorized by the revised Vienna classification: endoscopic, pathological, and immunophenotypic features.
abstract: BACKGROUND
  According to the revised Vienna classification, the surgical removal of gastric epithelial neoplasia category 3 (low-grade dysplasia) lesions is not necessary, whereas the removal of category 4 lesions (high-grade dysplasia and intramucosal cancer) is obligatory. However, approximately 15%-30% of low-grade adenomas/dysplasia progress to high-grade lesions or adenocarcinoma, and it is difficult to determine which lesions will advance to true malignancy. The aim of this study was to evaluate the endoscopic, pathological, and immunophenotypic differences between category 3 and 4 lesions according to the revised Vienna classification.
  
  
  METHODS
  All tissue samples were excised by endoscopic mucosal resection. Fifty-two category 3 tissue samples and 54 category 4 samples were evaluated by endoscopic findings; by pathology examination of the surrounding mucosa; and by CD10, MUC2, MUC5AC, MUC6, and RUNX3 immunohistochemical staining.
  
  
  RESULTS
  Univariate analysis showed that the size of the lesion, color change, ulceration, gastritis score of the surrounding mucosa, and positive expression of MUC6 were associated with category 4 lesions. Multivariate analysis showed that the size of the lesion, ulceration, and positive expression of MUC6 were strongly associated with category 4 lesions.
  
  
  CONCLUSIONS
  Lesions more than 17 mm in diameter or lesions that are associated with ulceration have the potential for malignant transformation. Positive immunoreactivity for MUC6 appears to be a complementary marker for malignant transformation of gastric epithelial neoplasia.
authors.affiliation: Department of Internal Medicine and Pathology, College of Medicine, the Catholic University of Korea, Seoul, Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Jung
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Chung
authors.forename: Kang-Moon
authors.initials: KM
authors.lastname: Lee
authors.forename: Chang Nyol
authors.initials: CN
authors.lastname: Paik
authors.forename: Ji Han
authors.initials: JH
authors.lastname: Jung
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Lee
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Chung
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000209:etiology* / Q000473:pathology; D000236:Adenoma / Q000145:classification / Q000473:pathology*; D000368:Aged; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology / Q000601:surgery; D005773:Gastroscopy / Q000379:methods; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D055272:Mucin-6 / Q000276:immunology; D015999:Multivariate Analysis; D011230:Precancerous Conditions / Q000145:classification / Q000473:pathology*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000145:classification / Q000209:etiology* / Q000473:pathology
chemical_list: C524734:MUC6 protein, human; D055272:Mucin-6