title: Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to gamma-aminobutyric acid and leucine.
abstract: The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing gamma-aminobutyric acid (GABA) and delta-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina.
authors.forename: Sabrina Beatriz
authors.initials: SB
authors.lastname: Cardillo
authors.forename: Mariana
authors.initials: M
authors.lastname: Bermúdez Moretti
authors.forename: Susana
authors.initials: S
authors.lastname: Correa García
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics; D007930:Leucine / Q000494:pharmacology*; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: C067023:DAL81 protein, S cerevisiae; D004268:DNA-Binding Proteins; D050485:GABA Plasma Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C092661:UGA3 protein, S cerevisiae; C513599:UGA4 protein, S cerevisiae; D005680:gamma-Aminobutyric Acid; D007930:Leucine