Characterization of an extracellular alkaline lipase from Pseudomonas mendocina M-37.
A strain of Pseudomonas mendocina producing extracellular lipase was isolated from soil. The bacterium accumulates lipase in culture fluid when grown aerobically at 30 °C for 24 h in a medium composed of olive oil (1%) as substrate. Pseudomonas mendocina lipase was optimally active at pH 9.0, temperature of 50 °C and was found to be stable between pH 7.0-11.0. The lipase was inhibited by detergents such as SDS and Tween-80. The enzyme was stable in various organic solvents tested with maximum stability in chloroform followed by toluene and exhibited 1-3 regiospecificity for hydrolytic reaction. This lipase was capable of hydrolyzing a variety of lipidic substrates and is mainly active towards synthetic triglycerides and fatty acid esters that possess a butyryl group. Metal ions like Mg(2+), Ca(2+) and Na(+) stimulated lipase activity, whereas, Cu(2+), Mn(2+) and Hg(2+) ions caused inhibition.
Dahiya
Praveen
P
Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar, Haryana, India.
Arora
Pooja
P
Chaudhury
Ashok
A
Chand
Subhash
S
Dilbaghi
Neeraj
N
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry; D044203:Pseudomonas mendocina / Q000201:enzymology*; D012997:Solvents / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature
D001426:Bacterial Proteins; D003902:Detergents; D008670:Metals; D012997:Solvents; D008049:Lipase