title: Application of response surface methodology and artificial neural networks for optimization of recombinant Oryza sativa non-symbiotic hemoglobin 1 production by Escherichia coli in medium containing byproduct glycerol.
abstract: Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 (OsHb1) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone, yeast extract, sodium chloride and byproduct glycerol from biodiesel production. Response surface methodology (RSM) and artificial neural networks (ANNs), followed by multiple response optimization through a desirability function were applied to evaluate the amount of OsHb1 produced. The results obtained by the application of ANNs were more reliable since better statistical parameters were obtained. The optimal conditions were (g L(-1)), tryptone, 42.69; yeast extract, 20.11; sodium chloride, 17.77; and byproduct glycerol, 0.33. A maximum recombinant protein concentration of 3.50 g L(-1) and a minimum biomass concentration of 18.48 g L(-1) were obtained under these conditions. Although the concentrations of tryptone, yeast extract and sodium chloride are relatively high, the increase in the yield with respect to biomass formed (Y(P/X)) overcomes this disadvantage.
authors.affiliation: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, Argentina.
authors.forename: Pablo C
authors.initials: PC
authors.lastname: Giordano
authors.forename: Hugo D
authors.initials: HD
authors.lastname: Martínez
authors.forename: Alberto A
authors.initials: AA
authors.lastname: Iglesias
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Beccaria
authors.forename: Héctor C
authors.initials: HC
authors.lastname: Goicoechea
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005990:Glycerol / Q000494:pharmacology*; D006454:Hemoglobins / Q000096:biosynthesis* / Q000302:isolation & purification; D016571:Neural Networks, Computer; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D011995:Recombination, Genetic / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D003470:Culture Media; D006454:Hemoglobins; D010940:Plant Proteins; C551014:non-symbiotic hemoglobin 1 protein, rice; D005990:Glycerol