[Technical optimization for extracting hypotensive active peptides from Agrocybe aegerita].
OBJECTIVE
To optimize the techniques for extracting hypotensive active peptides from Agrocybe aegerita.


METHODS
The effects of the liquid/solid ratio, extraction time and temperature, pH value of the initial liquid on the extraction percentage (EP) of the hypotensive active peptides were investigated, and inhibition percentage (IP) of the extracts on angiotensin I-converting enzyme was determined.


RESULTS
Optimal extraction of the hypotensive active peptides from Agrocybe aegerita was achieved with the liquid/solid ratio of 40:1, extraction time of 3 h, extraction temperature at 30 degrees Celsius;, and pH=8 of the initial liquid. The EP of the hypotensive active peptides from Agrocybe aegerita could reach 87.7% with IP of the extracts on angiotensin I-converting enzyme of 54.0%.


CONCLUSIONS
This method is simple and efficient for extracting hypotensive active peptides from Agrocybe aegerita.
Wu
Shan-gang
SG
College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Sun
Hong-na
HN
Sun
Jian-hua
JH
Liao
Dan-kui
DK
D055362:Agrocybe / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004044:Dietary Proteins / Q000302:isolation & purification / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology
D000959:Antihypertensive Agents; D004044:Dietary Proteins; D010455:Peptides