T vector bearing KillerRed protein marker for red/white cloning screening.
As a novel selection marker for DNA cloning, the genetically encoded photosensitizer KillerRed was used to achieve red/white cloning screening within the pZK18T T-vector system. KillerRed functioned without cofactors, inducers, or substrates. KillerRed-based red/white cloning screening was reliable in that bacteria containing DNA inserts that disrupt functional KillerRed expression form white colonies or red colonies as backgrounds. KillerRed simplifies assembly of customized vector and recombinant screening procedures. No special host or culture medium is required to amplify and prepare the vector in larger quantities. It makes high-throughput general-purpose cloning screening simpler, less expensive, and more effective.
Liu
Xuedong
X
Laboratory of Genetics and Molecular Biology, Northeast Forestry University, Harbin 150040, China.
Liu
Xing
X
Zhou
Yanna
Y
Zou
Dan
D
Shi
Rui
R
Li
Zaiyu
Z
Zheng
Dong
D
D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes; D005822:Genetic Vectors / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics
D015415:Biomarkers; D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins