title: Detection and genetic characterization of peste des petits ruminants virus in free-living bharals (Pseudois nayaur) in Tibet, China.
abstract: Peste des petits ruminants (PPR) is an important viral disease of sheep and goats. The wildlife hosts of PPR, which may play an important role in the epidemiology of this disease, are not well characterized. The research was undertaken to study the infection of PPR virus (PPRV) in free-living bharals (Pseudois nayaur) in Tibet, China. In 2007, PPRV infection was confirmed in two bharals in Rutog County of Tibet based on clinical signs and detection of PPRV RNA in tissue samples. In 2008, PPRV infection was found in one bharal in Ge'gyai County of Tibet by competitive ELISA, reverse transcription-polymerase chain reaction, and sequence analysis of PPRV fusion protein (F) and nucleoprotein (N) gene segments. The PPRV variant identified in infected bharal was closely related to other circulating PPRV variants recently identified in sheep and goats from Tibet. This is the first report of PPRV infection in free-living bharals.
authors.affiliation: China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, China. jingyuebao@yahoo.com.cn
authors.forename: Jingyue
authors.initials: J
authors.lastname: Bao
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wu
authors.forename: Peiwangjie
authors.initials: P
authors.lastname: Sang
authors.forename: Guozhen
authors.initials: G
authors.lastname: Wu
authors.forename: Guoyi
authors.initials: G
authors.lastname: Ding
authors.forename: Longciren
authors.initials: L
authors.lastname: Suo
authors.forename: Chunju
authors.initials: C
authors.lastname: Liu
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
authors.forename: Wenji
authors.initials: W
authors.lastname: Zhao
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D029021:Peste-des-Petits-Ruminants / Q000453:epidemiology* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics*; D010802:Phylogeny; D012418:Ruminants; D018609:Tibet / Q000453:epidemiology