Extending the alkene substrate range of vinyl chloride utilizing Nocardioides sp. strain JS614 with ethene oxide.
Nocardioides sp. strain JS614 grows on the C(2) alkenes ethene (Eth), vinyl chloride, and vinyl fluoride as sole carbon sources. The presence of 400-800 microM ethene oxide (EtO) extended the growth substrate range to propene (C(3)) and butene (C(4)). Propene-dependent growth of JS614 was CO(2) dependent and was prevented by the carboxylase/reductase inhibitor 2-bromoethanesulfonic acid, sodium salt (BES), while growth on Eth was not CO(2) dependent or BES sensitive. Although unable to promote growth, both propene and propene oxide (PrO)-induced expression of the genes encoding the alpha subunit of alkene monooxygenase (etnC) and epoxyethane CoM transferase (etnE) to similar levels as did Eth and EtO. Propene was transformed by Eth-grown and propene-grown/EtO-induced JS614 to PrO at a rate 4.2 times faster than PrO was consumed. As a result PrO accumulated in growth medium to 900 microM during EtO-induced growth on propene. PrO (50-100 microM) exerted inhibitory effects on growth of JS614 on both acetate and Eth, and on EtO-induced growth on Eth. However, higher EtO concentrations (300-400 microM) overcame the negative effects of PrO on Eth-dependent growth.
Taylor
Anne E
AE
Oregon State University, Corvallis, 97331, USA. anne.taylor@oregonstate.edu
Arp
Daniel J
DJ
Bottomley
Peter J
PJ
Semprini
Lewis
L
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism*; D000475:Alkenes / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism*; D010087:Oxides / Q000378:metabolism*; D014752:Vinyl Chloride / Q000378:metabolism*
D000475:Alkenes; D005030:Ethylenes; D010087:Oxides; C036216:ethylene; D014752:Vinyl Chloride