title: Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft.
abstract: BACKGROUND
  In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model.
  
  
  METHODS
  The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60.
  
  
  RESULTS
  The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone.
  
  
  CONCLUSIONS
  Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed.
authors.affiliation: Oral and Maxillofacial Surgery Unit, Hospital de Clinicas de P.A., School of Dentistry, UFRGS, Porto Alegre, RS, Brazil. epuricelli@uol.com.br
authors.forename: Edela
authors.initials: E
authors.lastname: Puricelli
authors.forename: Adriana
authors.initials: A
authors.lastname: Corsetti
authors.forename: Deise
authors.initials: D
authors.lastname: Ponzoni
authors.forename: Gustavo L
authors.initials: GL
authors.lastname: Martins
authors.forename: Mauro G
authors.initials: MG
authors.lastname: Leite
authors.forename: Luis A
authors.initials: LA
authors.lastname: Santos
mesh_terms: D000818:Animals; D001843:Bone Cements; D018786:Bone Substitutes; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002484:Cementation / Q000379:methods; D004195:Disease Models, Animal; D005269:Femur / Q000637:transplantation*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous
chemical_list: D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate