title: Antigenotoxic effect of allicin against estradiol-17beta-induced genotoxic damage in cultured mammalian cells.
abstract: Antigenotoxic activity of allicin, one of the sulphur compounds of garlic (Allium sativum) which possesses antioxidant and thiol disulphide exchange activity, was studied against estradiol-17beta-induced genotoxic damage using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) as parameters. Approximately 10, 20 and 40 microM of estradiol-17beta was tested for its genotoxic effect in the presence of metabolic activation and was found to be genotoxic at 20 and 40 microM. Approximately 20 microM of estradiol-17beta was treated along with 5, 10 and 15 microM of allicin, separately, in the presence of metabolic activation. Similar treatments were given with 40 microM of estradiol-17beta. Treatments along with allicin result in the reduction of CAs and SCEs, suggesting its anti-genotoxic activity in human lymphocytes in vitro against estradiol-17beta-induced genotoxic damage.
authors.affiliation: Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India. yasir_hasansiddique@rediffmail.com
authors.forename: Yasir Hasan
authors.initials: YH
authors.lastname: Siddique
authors.forename: Tanveer
authors.initials: T
authors.lastname: Beg
authors.forename: Gulshan
authors.initials: G
authors.lastname: Ara
authors.forename: Jyoti
authors.initials: J
authors.lastname: Gupta
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afzal
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity; D005260:Female; D006801:Humans; D012044:Regression Analysis; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000235:genetics; D013441:Sulfinic Acids / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004220:Disulfides; D004967:Estrogens; D013441:Sulfinic Acids; C006452:allicin; D004958:Estradiol