title: Associations between MHC genes and Puumala virus infection in Myodes glareolus are detected in wild populations, but not from experimental infection data.
abstract: We analysed the influence of MHC class II Dqa and Drb genes on Puumala virus (PUUV) infection in bank voles (Myodes glareolus). We considered voles sampled in five European localities or derived from a previous experiment that showed variable infection success of PUUV. The genetic variation observed in the Dqa and Drb genes was assessed by using single-strand conformation polymorphism and pyrosequencing methods, respectively. Patterns were compared with those obtained from 13 microsatellites. We revealed significant genetic differentiation between PUUV-seronegative and -seropositive bank voles sampled in wild populations, at the Drb gene only. The absence of genetic differentiation observed at neutral microsatellites confirmed the important role of selective pressures in shaping these Drb patterns. Also, we found no significant associations between infection success and MHC alleles among laboratory-colonized bank voles, which is explained by a loss of genetic variability that occurred during the captivity of these voles.
authors.affiliation: INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988 Montferrier-sur-Lez cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guivier
authors.forename: Maxime
authors.initials: M
authors.lastname: Galan
authors.forename: Pierre-Jean G
authors.initials: PJ
authors.lastname: Malé
authors.forename: Eva R
authors.initials: ER
authors.lastname: Kallio
authors.forename: Liina
authors.initials: L
authors.lastname: Voutilainen
authors.forename: Heikki
authors.initials: H
authors.lastname: Henttonen
authors.forename: Gert E
authors.initials: GE
authors.lastname: Olsson
authors.forename: Ake
authors.initials: A
authors.lastname: Lundkvist
authors.forename: Katrien
authors.initials: K
authors.lastname: Tersago
authors.forename: Denis
authors.initials: D
authors.lastname: Augot
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cosson
authors.forename: Nathalie
authors.initials: N
authors.lastname: Charbonnel
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000821:virology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D029262:Puumala virus / Q000276:immunology / Q000472:pathogenicity*; D012376:Rodent Diseases / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D000949:Histocompatibility Antigens Class II