title: Stability curves of laboratory evolved thermostable mutants of a Bacillus subtilis lipase.
abstract: Shape of the protein stability curves changes to achieve higher melting temperature. Broadly, these changes have been classified as upward shift (increased G(s)), rightward shift (increase in T(s)) and flattening of the stability curves (decrease in C(p)). Comparative studies on homologous mesophilic-thermophilic protein pairs highlighted the differential contribution of these three strategies amongst proteins. But unambiguous way of identification of the strategies, which will be preferred for a protein, is still not achieved. We have performed comparative thermodynamic studies using differential scanning calorimeter (DSC) on thermostable variants of a lipase from Bacillus subtilis. These variants are products of 1, 2, 3 and 4 rounds of directed evolution and harbor mutations having definite contribution in thermostability unlike natural thermophilic proteins. We have shown that upward and rightward shift in stability curves are prime strategies in this lipase. Our results along with that from the other study on laboratory evolved xylanase A suggest that optimization of suboptimal thermodynamic parameters is having a dominant influence in selection of thermodynamic strategies for higher thermostability.
authors.affiliation: Centre for Cellular and Molecular Biology, Council for Scientific and Industrial research, Uppal Road, Hyderabad-500007, India.
authors.forename: Md Zahid
authors.initials: MZ
authors.lastname: Kamal
authors.forename: Shoeb
authors.initials: S
authors.lastname: Ahmad
authors.forename: Poornima
authors.initials: P
authors.lastname: Yedavalli
authors.forename: Nalam Madhusudhana
authors.initials: NM
authors.lastname: Rao
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D002152:Calorimetry, Differential Scanning; D019020:Directed Molecular Evolution; D004795:Enzyme Stability; D006358:Hot Temperature; D008049:Lipase / Q000737:chemistry* / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D013816:Thermodynamics
chemical_list: D008049:Lipase