Chloride channel-dependent copper acquisition of laccase in the basidiomycetous fungus Cryptococcus neoformans.
The CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated pH. This study reports that copper homeostasis was impaired in the clc-a mutant. This was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption spectrometry. CLC-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in C. neoformans.
Zhu
ChangWei
C
State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Jiang
Nan
N
State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Xiao
DongGuang
D
Tianjin Industrial Microbiology Key Lab, College of Bioengineering, Tianjin University of Science & Technology, Tianjin, 300457, China.
Pan
Jiao
J
State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Zhu
XuDong
X
State Key Program of Microbiology and Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China. xudong82@nankai.edu.cn.
D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism* / Q000493:pharmacokinetics; D003455:Cryptococcus neoformans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic
D018118:Chloride Channels; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins; D003300:Copper; D042845:Laccase