title: Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis.
abstract: The maturation state of dendritic cells (DC) is regarded as a control point for the induction of peripheral tolerance or autoimmunity. Experimental autoimmune orchitis (EAO) serves as a model to investigate inflammatory-based testicular impairment, which ranks as a significant cause of male infertility. This work aimed to determine whether DC enrichment occurs organotypically in testicular draining lymph nodes (TLN) compared with LN draining the site of immunization (ILN) and thus contributes to the pathogenesis of autoimmune orchitis. In this regard, we quantified and characterized the DC from TLN and ILN in rats with EAO. Flow cytometric analysis showed a significant increase in the percentage of DC (OX62+) only in TLN from EAO rats compared with normal (N) and adjuvant control (C) groups. The number of DC from ILN and TLN expressing CD80, CD86 and major histocompatibility complex (MHC) II was comparable among N, C and experimental (E) groups at 30 and 50 days after the first immunization. However, TLN DC from EAO rats (50 days) showed an increase in mean fluorescence intensity for MHC II compared with N, C and E groups (30 days). The mRNA expression level of IL-10 and IL-12p35 was significantly upregulated in enriched DC fraction from TLN in EAO rats with no significant changes observed in ILN DC. The expression of IL-23p19 mRNA remained unchanged. Functional data, using proliferation assays showed that EAO-DC from TLN, but not from ILN, significantly enhanced the proliferation of naïve T cells compared with C-DC. In summary, our data suggest that the DC in TLN from orchitis rats are mature, present antigens to T cells and stimulate an autoimmune response against testicular antigens, thus causing immunological infertility.
authors.affiliation: Institute for Research in Reproduction, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: V A
authors.initials: VA
authors.lastname: Guazzone
authors.forename: S
authors.initials: S
authors.lastname: Hollwegs
authors.forename: M
authors.initials: M
authors.lastname: Mardirosian
authors.forename: P
authors.initials: P
authors.lastname: Jacobo
authors.forename: H
authors.initials: H
authors.lastname: Hackstein
authors.forename: M
authors.initials: M
authors.lastname: Wygrecka
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: A
authors.initials: A
authors.lastname: Meinhardt
authors.forename: L
authors.initials: L
authors.lastname: Lustig
authors.forename: M
authors.initials: M
authors.lastname: Fijak
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D051940:B7-2 Antigen / Q000096:biosynthesis / Q000235:genetics; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D007114:Immunization; D007248:Infertility, Male; D007249:Inflammation; D016753:Interleukin-10 / Q000235:genetics; D053765:Interleukin-12 Subunit p35 / Q000235:genetics; D053760:Interleukin-23 Subunit p19 / Q000235:genetics; D008297:Male; D009920:Orchitis / Q000276:immunology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology*; D013737:Testis / Q000276:immunology* / Q000473:pathology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D053765:Interleukin-12 Subunit p35; D053760:Interleukin-23 Subunit p19; D012333:RNA, Messenger; D016753:Interleukin-10