Effects of albendazole on Litomosoides chagasfilhoi (Nematoda: Filarioidea) females in vivo.
Gerbils (Meriones unguiculatus) experimentally infected with Litomosoides chagasfilhoi were treated with a single oral dose of 40 or 80 mg of albendazole, respectively. Observation of the microfilaremia after the treatment showed that both single oral doses of albendazole decreased the microfilaremia in L. chagasfilhoi infection. The body wall was composed of a cuticle, a hypodermis, and a muscular layer, and treated nematodes showed no morphological alterations. The ultrastructural alterations produced by treatment with 40 mg of albendazole included a higher number of membrane invaginations in the basal labyrinth of the uterine epithelium and the presence of myelin figures in this region. Inside the uterus, most embryos and microfilariae were disintegrated. The treatment with 80 mg of albendazole did not produce alterations in the uterine wall, and the number of vesicles near the microfilariae sheath was smaller than that observed in the untreated and in the 40-mg treatment groups. However, all the microfilariae observed in the uterus were extensively damaged with cytoplasmic vacuolization and cellular degeneration. No alterations in the intestinal cells were observed after treatment with 40 or 80 mg of albendazole. The present study contributes to the knowledge of albendazole's effects in filariids and demonstrates the potential embryotoxic and microfilaricidal consequences of this drug.
Cárdenas
Melissa Q
MQ
Laboratório de Biologia de Helmintos Otto Wucherer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900, Rio de Janeiro, RJ, Brazil. melissaq@ioc.fiocruz.br
Oliveira-Menezes
Aleksandra
A
Lanfredi
Reinalda M
RM
D000284:Administration, Oral; D015766:Albendazole / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology*; D001769:Blood / Q000469:parasitology; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D004195:Disease Models, Animal; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005368:Filariasis / Q000188:drug therapy; D005370:Filarioidea / Q000187:drug effects* / Q000648:ultrastructure*; D005849:Gerbillinae / Q000469:parasitology; D007422:Intestines / Q000187:drug effects / Q000648:ultrastructure; D014599:Uterus / Q000187:drug effects / Q000648:ultrastructure
D000871:Anthelmintics; D015766:Albendazole