A simple scanless two-photon fluorescence microscope using selective plane illumination.
We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.
Palero
Jonathan
J
ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels (Barcelona), Spain.
Santos
Susana I C O
SI
Artigas
David
D
Loza-Alvarez
Pablo
P
D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D008029:Lighting / Q000295:instrumentation*; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009132:Muscles / Q000378:metabolism; D010614:Pharynx / Q000378:metabolism; D017785:Photons
D003374:Coumarins; D008164:Luminescent Proteins; C030123:coumarin