Adaptor protein GULP is involved in stabilin-1-mediated phagocytosis.
The clearance of apoptotic cells is critical during cellular homeostasis as well as inflammation resolution. Recently, we found that stabilin-1 is a phagocytic receptor that is involved in the clearance of apoptotic cells. However, the downstream signaling pathway of stabilin-1-mediated phagocytosis remains to be investigated. Here we identify that GULP is able to specifically interact with the NPxF/Y motif of stabilin-1 cytoplasmic region. The PTB domain of GULP is necessary for interaction with stabilin-1. GULP is enriched around PS-coated beads for phagocytosis and co-localized with stabilin-1. Downregulation of endogenous GULP expression decreased stabilin-1-mediated phagocytosis. Thus, these results indicate that GULP functions as an adaptor protein for stabilin-1-mediated phagocytosis.
Park
Seung-Yoon
SY
Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
Kim
Sang-Yeob
SY
Kang
Kae-Bok
KB
Kim
In-San
IS
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D055785:Gene Knockdown Techniques; D006801:Humans; D008969:Molecular Sequence Data; D010587:Phagocytosis; D017434:Protein Structure, Tertiary; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D015816:Cell Adhesion Molecules, Neuronal; C454119:GULP1 protein, human; D016201:Receptors, Lymphocyte Homing; C449926:STAB1 protein, human