title: Chemokine/chemokine receptor interactions in extramedullary leukaemia of the skin in childhood AML: differential roles for CCR2, CCR5, CXCR4 and CXCR7.
abstract: Chemokine receptor/ligand interactions orchestrate the migration of cells to peripheral tissues such as the skin. We analysed chemokine receptor expression by acute myeloid leukaemic (AML) cells present in peripheral blood (n = 7), bone marrow (n = 6), or skin (n = 11) obtained from 15 paediatric AML patients with skin involvement and in 10 AML patients without skin involvement. High percentages of circulating CCR2(pos) AML cells were only detected in patients with extramedullary disease. Skin-residing AML cells displayed a different set of receptors in situ, namely: CCR5, CXCR4, CXCR7 and CX3CR1. These results suggest the involvement of different chemokine/chemokine receptor interactions in homing and retention of AML blasts in the skin.
authors.affiliation: Division of Immunology, Haematology, Oncology, Bone Marrow Transplantation and Autoimmune Diseases, Department of Paediatrics, Leiden University Medical Centre, Leiden, The Netherlands.
authors.forename: Claudia M J M
authors.initials: CM
authors.lastname: Faaij
authors.forename: Annemieke J
authors.initials: AJ
authors.lastname: Willemze
authors.forename: Tom
authors.initials: T
authors.lastname: Révész
authors.forename: Melania
authors.initials: M
authors.lastname: Balzarolo
authors.forename: Cornelis P
authors.initials: CP
authors.lastname: Tensen
authors.forename: Manja
authors.initials: M
authors.lastname: Hoogeboom
authors.forename: Maarten H
authors.initials: MH
authors.lastname: Vermeer
authors.forename: Elisabeth
authors.initials: E
authors.lastname: van Wering
authors.forename: Christian M
authors.initials: CM
authors.lastname: Zwaan
authors.forename: Gertjan J L
authors.initials: GJ
authors.lastname: Kaspers
authors.forename: Colin
authors.initials: C
authors.lastname: Story
authors.forename: Astrid G S
authors.initials: AG
authors.lastname: van Halteren
authors.forename: Jaak M
authors.initials: JM
authors.lastname: Vossen
authors.forename: R Maarten
authors.initials: RM
authors.lastname: Egeler
authors.forename: Maarten J D
authors.initials: MJ
authors.lastname: van Tol
authors.forename: Nicola E
authors.initials: NE
authors.lastname: Annels
mesh_terms: D000293:Adolescent; D000073883:CX3C Chemokine Receptor 1; D016022:Case-Control Studies; D018925:Chemokines / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D017254:Leukemic Infiltration / Q000473:pathology*; D008297:Male; D054390:Receptors, CCR2 / Q000032:analysis; D019713:Receptors, CCR5 / Q000032:analysis; D054387:Receptors, CXCR / Q000032:analysis; D019718:Receptors, CXCR4 / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis*; D012867:Skin / Q000737:chemistry / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: C501896:ACKR3 protein, human; C515334:CCR2 protein, human; D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D018925:Chemokines; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D054387:Receptors, CXCR; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine