title: Baculovirus treatment fully protects mice against a lethal challenge of FMDV.
abstract: Foot-and-mouth disease virus (FMDV) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. FMDV is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. It has been demonstrated that FMDV A/Arg/2001 causes the death of adult C57Bl/6 mice within 72h. We evaluated the capacity of Autographa californica nuclear polyhedrosis virus (AcNPV), an insect virus with potent innate immunostimulating effects, to promote early protection against FMDV A/Arg/2001 challenge in C57Bl/6 mice. Groups of 8-9 weeks old female mice were injected intravenously with AcNPV and challenged with a lethal dose of FMDV at different times post-administration. Our results showed that pretreatment of mice with a single injection of AcNPV 3h or 3 days before FMDV challenge resulted in complete abrogation of mortality and complete or partial suppression of viremia, respectively. Furthermore, no signs of disease were observed. AcNPV could be a valuable tool to improve the design of a novel vaccine that protects as an adjuvant at early times post-vaccination.
authors.affiliation: Instituto de Biotecnología, CNIA, INTA, Castelar, CC25 B1712WAA Buenos Aires, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Molinari
authors.forename: Soledad
authors.initials: S
authors.lastname: García-Nuñez
authors.forename: M José
authors.initials: MJ
authors.lastname: Gravisaco
authors.forename: Elisa
authors.initials: E
authors.lastname: Carrillo
authors.forename: Analía
authors.initials: A
authors.lastname: Berinstein
authors.forename: Oscar
authors.initials: O
authors.lastname: Taboga
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000276:immunology*; D001691:Biological Therapy / Q000379:methods*; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000254:growth & development* / Q000276:immunology*; D007313:Insecta / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D016019:Survival Analysis; D014766:Viremia / Q000517:prevention & control