title: Early infection during burn-induced inflammatory response results in increased mortality and p38-mediated neutrophil dysfunction.
abstract: Following burn injury, the host is susceptible to bacterial infections normally cleared by healthy patients. We hypothesized that during the systemic immune response that follows scald injury, the host's altered immune status increases infection susceptibility. Using a murine model of scald injury under inhaled anesthesia followed by intraperitoneal infection, we observed increased neutrophil numbers and function at postburn day (PBD) 1 compared with sham-burned and PBD4 mice. Further, increased mortality, bacteremia, and serum IL-6 were observed in PBD1 mice after Pseudomonas aeruginosa (PA) infection compared with sham-burned and PBD4 mice infected with PA. To examine these disparate responses, we investigated neutrophils isolated at 5 and 24 h following PA infection from PBD1 and sham-burned mice. Five hours after infection, there was no significant difference in number of recruited neutrophils; however, neutrophils from injured mice had decreased activation, active-p38, and oxidative burst compared with sham-burned mice. In direct contrast, 24 h after infection, we observed increased numbers, active-p38, and oxidative burst of neutrophils from PBD1 mice. Finally, we demonstrated that in neutrophils isolated from PBD1 mice, the observed increase in oxidative burst was p38 dependent. Altogether, neutrophil activation and function from thermally injured mice are initially delayed and later exacerbated by a p38-dependent mechanism. This mechanism is likely key to the observed increase in bacterial load and mortality of PBD1 mice infected with PA.
authors.affiliation: Department of Research, Shriner's Hospital for Children, Cincinnati, Ohio, USA.
authors.forename: Samuel G
authors.initials: SG
authors.lastname: Adediran
authors.forename: Derrick J
authors.initials: DJ
authors.lastname: Dauplaise
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Kasten
authors.forename: Johannes
authors.initials: J
authors.lastname: Tschöp
authors.forename: Jonathan
authors.initials: J
authors.lastname: Dattilo
authors.forename: Holly S
authors.initials: HS
authors.lastname: Goetzman
authors.forename: Lisa G
authors.initials: LG
authors.lastname: England
authors.forename: Cindy M
authors.initials: CM
authors.lastname: Cave
authors.forename: Chad T
authors.initials: CT
authors.lastname: Robinson
authors.forename: Charles C
authors.initials: CC
authors.lastname: Caldwell
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D007249:Inflammation / Q000150:complications*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000201:enzymology* / Q000502:physiology*; D011552:Pseudomonas Infections / Q000150:complications* / Q000276:immunology / Q000401:mortality; D011550:Pseudomonas aeruginosa; D016897:Respiratory Burst; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases