Is the secondary putative RNA-RNA interaction site relevant to GcvB mediated regulation of oppA mRNA in Escherichia coli?
GcvB is a non-coding RNA that regulates oppA mRNA in different bacterial species by binding a GcvB GU-rich region named R1 to oppA mRNA. A secondary putative interaction site (PS1) was identified in this study that is able to form a second nearly perfect 10 base-pair duplex between these two RNAs in Escherichia coli. In this work, we have studied whether the formation of a second interaction site could help stabilize the previously reported GcvB/oppA complex. Several mutations and the full deletion of PS1 were engineered. None of these modifications affected the ability of GcvB to control OppA expression. Therefore the second, putative, interaction site appears to be unnecessary for the regulatory function of GcvB with regard to its oppA target mRNA.
Busi
Florent
F
UPR CNRS n° 9073, Affiliated with Université Paris Diderot-Paris 7 Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Le Derout
Jacques
J
Cerciat
Marie
M
Régnier
Philippe
P
Hajnsdorf
Eliane
E
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000235:genetics*; D009154:Mutation; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000378:metabolism
D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C499290:OppA protein, E coli; D012329:RNA, Bacterial; D012333:RNA, Messenger; D022661:RNA, Untranslated; D000594:Amino Acid Oxidoreductases; C087190:GcvP protein, E coli