title: Effect of ankaferd blood stopper on hemostasis and histopathological score in experimental liver injury.
abstract: OBJECTIVE
  To investigate intra-abdominal, local histopathological and hemostatic effects of Ankaferd (ABS) in an experimental liver injury.
  
  
  METHODS
  Forty rats were randomly assigned to the primary suture group (PS) (n = 15), ABS group (n = 15) and control group (n = 10). A wedge resection was performed on the left lobe of the liver. In the primary suture group, the liver was sutured with polypropylene material, while in ABS group the liver surface was covered with ABS. Adhesion, histo-pathological scores and hydroxyproline levels were measured postoperatively on day 3 and day 15.
  
  
  RESULTS
  The mean bleeding time was 7.5 sec shorter in the ABS group (6.5 sec-13.5 sec) and 107 sec shorter in PS group. AST, ALT, total bilirubin and ALP values complete blood count (CBC) did not change. Intra-abdominal adhesions were the same in PS and ABS groups on 3rd (2.20 +/- 1.30 vs 2.0 +/- 1.11) and 15th (1.60 +/- 0.54 vs 1.25 +/- 0.7) days postoperatively. Histopathological scores were better in the ABS group than in the primary suture group on 3rd (2.5 +/- 0.5 vs 5.25 +/- 0.2, p = 0.006) and 15th (1.65 +/- 1.7 vs 3.15 +/- 1.0, p = 0.025) days postoperatively. Hydroxyproline levels were higher in ABS group on postoperative 15th day (17.12 microg/tissue vs 13.69 microg/tissue; p = 0.005).
  
  
  CONCLUSIONS
  These data suggest that ABS in experimental liver trauma causes favorable histopathological scores and shorter hemostasis time and higher hydroxyproline levels (Tab. 2, Fig. 2, Ref. 35).
authors.affiliation: Department of General Surgery, Okmeydani Training and Research Hospital, Istanbul, Turkey. mukalayci@hotmail.com
authors.forename: Mustafa Uygar
authors.initials: MU
authors.lastname: Kalayci
authors.forename: Aliye
authors.initials: A
authors.lastname: Soylu
authors.forename: Hasan Erol
authors.initials: HE
authors.lastname: Eroglu
authors.forename: Dilek
authors.initials: D
authors.lastname: Kubilay
authors.forename: Banu
authors.initials: B
authors.lastname: Sancak
authors.forename: Ceyhan
authors.initials: C
authors.lastname: Ugurluoglu
authors.forename: Ugur
authors.initials: U
authors.lastname: Ercin
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Koca
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Karatepe
mesh_terms: D000818:Animals; D006470:Hemorrhage / Q000188:drug therapy*; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000627:therapeutic use*; D008099:Liver / Q000293:injuries* / Q000473:pathology; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats
chemical_list: D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper