In vitro evaluation of the effects of cysticidal drugs in the Taenia crassiceps cysticerci ORF strain using the fluorescent CellTracker CMFDA.
Using a murine model of cysticercosis caused by the Taenia crassiceps ORF strain, we developed a fluorescent quantitative evaluation of the action of two well known anti-helminthic drugs: albendazole sulfoxide and praziquantel. The fluorescence emitted by a biotransformed CellTracker Probe known as CellTracker Green CMFDA in the vesicular fluids of cysticerci was estimated, and the results were compared with macroscopic observations of the parasites. The pharmacological EC(50) value of each drug and changes in the level of biotransformation of the fluorescent tracker caused by the drugs could be easily calculated. These drug-induced changes in biotransformation could be related to changes in the GSH/GSSG ratio of parasites. Both the cysticercosis murine model and the CMFDA biotransformation assay could be used as an in vitro screening method to evaluate potential or well known cysticidal drugs.
Trejo-Chávez
Hector
H
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edif A, 2do Piso, Ciudad Universitaria, CP 04510, Ciudad de México, Mexico.
García-Vilchis
David
D
Reynoso-Ducoing
Olivia
O
Ambrosio
Javier R
JR
D015766:Albendazole / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001711:Biotransformation; D003552:Cysticercus / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005452:Fluoresceins / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D011223:Praziquantel / Q000494:pharmacology*
D000871:Anthelmintics; D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein; D011223:Praziquantel; D015766:Albendazole; C027186:albendazole sulfoxide