A Ru(II)-TAP complex, photoreagent for tryptophan-containing peptides: structure of the covalent photoadduct.
We report the first structure determination of a covalent photoadduct between a Ru(II)-tap complex and a tryptophan-containing peptide (AlaTrpAla) by mass spectrometry and NMR spectroscopy. Ru(II)-tap complexes could thus be exploited as photodamaging agents of Trp-containing polypeptides or proteins.
Gauthier
Nicolas
N
Service de Chimie Organique et Photochimie, Université libre de Bruxelles, 50 Av. F.D. Roosevelt, B-1050 Bruxelles, Belgium.
De Winter
Julien
J
Gerbaux
Pascal
P
Moucheron
Cécile
C
Luhmer
Michel
M
Kirsch-De Mesmaeker
Andrée
A
D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010616:Phenanthrenes / Q000737:chemistry* / Q000378:metabolism*; D055668:Photochemical Processes; D014364:Tryptophan / Q000737:chemistry* / Q000378:metabolism*
D009842:Oligopeptides; D009942:Organometallic Compounds; D010616:Phenanthrenes; C076699:ruthenium-tris-1,4,5,8-tetraazaphenanthrene; D014364:Tryptophan