title: Determination of formaldehyde in aqueous solutions by a novel fluorescence energy transfer system.
abstract: An efficient fluorescence energy transfer (FET) system between CePO(4):Tb(3+) nanocrystals as donor and 6-mercapto-5-triazole[4,3-b]-S-tetrazine as acceptor was built. CePO(4):Tb(3+) nanocrystals were synthesized in aqueous solutions, and characterized by transmission electron microscopy, electron diffraction pattern spectroscopy and spectrofluorometry. Under alkaline conditions, 6-mercapto-5-triazole[4,3-b]-S-tetrazine was generated by redox reaction of formaldehyde (HCHO) with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT) at room temperature. The degree of the overlap was effective for FET between the emission spectrum of CePO(4):Tb(3+) and the absorption spectrum of 6-mercapto-5-triazole[4,3-b]-S-tetrazine. Based on this system, a simple and sensitive fluorescence method for the selective determination of formaldehyde in aqueous solutions was developed. Under optimal conditions, the quenched fluorescence intensity increased linearly with the concentration of HCHO ranging from 1.03 x 10(-9) to 1.03 x 10(-5) mg mL(-1) with a correlation coefficient of 0.9976. The limit of detection was 1.65 x 10(-10) mg mL(-1). Compared with several methods, the proposed method had a wider linear range, higher selectivity and sensitivity. Moreover, analytical application of the method was demonstrated by water samples.
authors.affiliation: Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, China. wanglun@mail.ahnu.edu.cn
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
authors.forename: Cai-Ling
authors.initials: CL
authors.lastname: Zhou
authors.forename: Hong-Qi
authors.initials: HQ
authors.lastname: Chen
authors.forename: Jing-Guo
authors.initials: JG
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.forename: Bo
authors.initials: B
authors.lastname: Ling
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005557:Formaldehyde / Q000032:analysis*; D012996:Solutions; D014867:Water / Q000737:chemistry
chemical_list: D012996:Solutions; D014867:Water; D005557:Formaldehyde