Multiphoton excitation characteristics of cellular fluorophores of human skin in vivo.
In vivo multiphoton tomography with a wavelength-tunable femtosecond laser has been performed to investigate the autofluorescence intensity of major endogenous fluorophores of human skin in dependence on the excitation wavelength. In high-resolution multiphoton images of different skin layers, clear trends were found for fluorophores like keratin, NAD(P)H, melanin as well as for the elastin and collagen networks. The analysis of the measurements is supplemented by additional measurements of fluorescence lifetime imaging and signal-decay curves by time-correlated single-photon counting.
Breunig
Hans Georg
HG
JenLab GmbH, Campus A1.2, 66123 Saarbrücken, Germany. breunig@jenlab.de
Studier
Hauke
H
König
Karsten
K
D003094:Collagen / Q000737:chemistry; D004549:Elastin / Q000737:chemistry; D005260:Female; D005453:Fluorescence; D006801:Humans; D017785:Photons; D012867:Skin / Q000033:anatomy & histology / Q000737:chemistry*; D014054:Tomography / Q000379:methods*
D003094:Collagen; D004549:Elastin