title: Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs.
abstract: BACKGROUND
  To find new, crude anti-obesity drugs from natural sources through the inhibition of adsorption of dietary lipids, in vitro porcine pancreatic lipase (PPL; triacylglycerol lipase, EC 3.1.1.3) inhibitory tests were carried out on selected plants with weight-reducing or related potential, used in Chinese traditional medicine.
  
  
  METHODS
  The methanolic extracts of 37 traditional Chinese herbal medicines of different families were assayed for their in vitro activity against PPL by using spectrophotometry with 2,4-dinitrophenyl butyrate as a synthetic substrate. Coexistent phytochemicals, or those present in high levels, in the 3 most promising Chinese herbs were tested for their anti-lipase activity.
  
  
  RESULTS
  Extracts from 2 herbs, Prunella vulgaris L. (Labiatae) and Rheum palmatum L. (Polygonaceae), at a concentration of 200 mg/mL, significantly inhibited PPL-by 74.7% and 53.8%, respectively. Quercetin exhibited better activity (27.4%) than all the other phytochemicals at a final concentration of 25 mg/mL in the assay system, followed by luteolin, with an activity of 17.3%.
  
  
  CONCLUSIONS
  The results support the view that herbs represent a rich source of anti-lipase compounds. The screening of the methanolic extracts of 37 Chinese medicinal plants in vitro led to the identification of several extracts with potential activity against PPL, in particular, P. vulgaris and R. palmatum. We also found that several monomeric chemicals in these herbs exhibited good or moderate activity against PPL. To the best of our knowledge, these traditional Chinese herbal medicines or phytochemicals have not been previously screened for their lipase inhibitory activity.
authors.affiliation: Research Centre for Natural Medicinal Chemistry, College of Science, Yangling, Shaanxi, China.
authors.forename: Cheng-Dong
authors.initials: CD
authors.lastname: Zheng
authors.forename: Ya-Qing
authors.initials: YQ
authors.lastname: Duan
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Gao
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Ruan
mesh_terms: D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D008516:Medicine, Chinese Traditional
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D008049:Lipase