Leishmania (Viannia) braziliensis: immunoblotting analysis for the detection of IgG subclasses in the diagnosis of symptomatic and asymptomatic dogs.
During a seroepidemiological survey 2004-2006 from areas in Brazil endemic for American cutaneous leishmaniasis (ACL), serum samples from 10 dogs with ulcerated cutaneous lesions (S-ACL) and 52 asymptomatic dogs (AS-ACL) of unknown age and breed living in areas endemic for ACL were monitored for 1 year for ulcerated cutaneous lesions and immunoblotting using peroxidase-conjugated secondary anti-IgG, anti-IgG1 and anti-IgG2 dog antibodies. We reported that antibodies against Leishmania (Viannia) braziliensis in the sera of 22/52 dogs with asymptomatic disease showed intense reactivity to peptides larger than 66 kDa. We believe that dogs harboring subclinical amastigotes show an immunoblotting profile similar to that of symptomatic animals because a dog with self-healing presented antigens greater than 66 kDa. Such patterns can be exploited for diagnostic and epidemiological research for leishmaniasis.
Zanini
Marcos Santos
MS
Departamento de Medicina Veterinária, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Espírito Santo, Vitória 29040-091, Brazil. zanini@cca.ufes.br
Viana
Kelvinson Fernandes
KF
Reis
Alexandre Barbosa
AB
Campos
Diefrey Ribeiro
DR
Mussi
Jamili Maria Suhet
JM
Zanini
Surama
S
Lemos
Elenice Moreira
EM
D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D015151:Immunoblotting / Q000662:veterinary*; D007074:Immunoglobulin G / Q000145:classification*; D007892:Leishmania braziliensis / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D007074:Immunoglobulin G