Self-assembled monolayer as a pre-concentrating receptor for selective serotonin sensing.
We describe a novel medium, a captopril/thiophenol (Capt/TP) mixed self-assembled monolayer (SAM) formed on gold, for selectively pre-concentrating serotonin (5-hydroxytryptamine, 5-HT). The 5-HT molecules were shown to be selectively captured via formation of a strong complex, providing anodic stripping currents upon anodic scan or drastic increases in charge-transfer resistances for a redox probe, Fe(CN)(6)(3-/4-) pair, due to their blocking effects. The 5-HT molecules thus collected were subsequently detected by anodic stripping differential pulse voltammetry (ASDPV) or electrochemical impedance spectroscopy (EIS). The detection limit was 1.2 when detected by EIS and 28 nM with ASDPV. 5-HT was shown to be determined in physiological matrices containing ascorbic acid, dopamine, and other commonly encountered neurotransmitters.
Mozaffari
Seyed Ahmad
SA
Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, Gyeongbuk, Republic of Korea.
Chang
Taihyun
T
Park
Su-Moon
SM
D015374:Biosensing Techniques / Q000295:instrumentation*; D002216:Captopril / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010636:Phenols / Q000737:chemistry*; D012701:Serotonin / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
D010636:Phenols; D013438:Sulfhydryl Compounds; D012701:Serotonin; C042983:thiophenol; D002216:Captopril