Specific effects exerted by B-lymphoproliferative diseases on peripheral T-lymphocyte protein expression.
A proteomic approach was applied to study the protein expression profile of peripheral T-cells derived from patients at the onset of different B-lymphoproliferative diseases, because a rising interest in specific actions played by T-cells in such pathologies has emerged. Decreased levels of profilin-1 and cofilin-1 and increased levels of coronin1A and prohibitin were found in patients, compared with healthy controls. The protein-protein interaction network of these proteins was studied using a web-based bioinformatics tool, highlighting the actin cytoskeleton regulation as the main biological process involved in peripheral T-cells of such patients. Unsupervised cluster analysis of protein expression data shows that the recorded alteration of T-cell proteome was specifically induced by B-cell pathologies.
Borro
Marina
M
2nd Faculty of Medicine, Sant'Andrea Hospital, Sapienza University of Rome, Italy.
Gentile
Giovanna
G
De Luca
Ottavia
O
Torre
Maria Simona
MS
Aimati
Laura
L
Tatarelli
Caterina
C
Antonietta Aloe Spiriti
Maria
M
Christina Cox
Maria
M
Simmaco
Maurizio
M
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016000:Cluster Analysis; D051338:Cofilin 1 / Q000097:blood; D005260:Female; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D051304:Profilins / Q000097:blood; D040901:Proteomics / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism*
D014408:Biomarkers, Tumor; C495810:CFL1 protein, human; D051338:Cofilin 1; D009363:Neoplasm Proteins; D051304:Profilins