Inhibition of human cytomegalovirus infection by IE86-specific short hairpin RNA-mediated RNA interference.
To develop a gene therapeutic method for human cytomegalovirus (HCMV), the IE86 specific short hairpin (sh) RNA expressing vector was constructed and subsequently transfected into MRC-5 cells. After infection of these cells with HCMV AD169, expression of IE86 was reduced strikingly as compared to the control. In addition, the inhibitory effect corresponded to a decrease in viral DNA replication and the virus-induced cytopathic effect. Measurement of the virus yield demonstrated that infection of cells expressing IE86-specific shRNA resulted in suppression of the formation of infectious viral progeny. These observations indicate that IE86 can be used as an effective target against HCMV infection using RNA interference (RNAi) technology, which provides new possibilities for anti-HCMV studies.
Bai
Zhiqiang
Z
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, China.
Li
Ling
L
Wang
Bin
B
Liu
Zhijun
Z
Liu
Haiyan
H
Jiang
Guangyu
G
Wang
Haitao
H
Yan
Zhiyong
Z
Qian
Dongmeng
D
Ding
Shouyi
S
Song
Xuxia
X
D002460:Cell Line; D003587:Cytomegalovirus / Q000378:metabolism / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics*; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000172:deficiency* / Q000235:genetics*; D055029:Inverted Repeat Sequences; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics*; D014162:Transfection; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D015534:Trans-Activators