Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.
Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs ("TiPS") retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages.
Brown
Matthew E
ME
Department of Research and Development, Cellular Dynamics International, Inc., Madison, Wisconsin, United States of America.
Rondon
Elizabeth
E
Rajesh
Deepika
D
Mack
Amanda
A
Lewis
Rachel
R
Feng
Xuezhu
X
Zitur
Laura Jo
LJ
Learish
Randall D
RD
Nuwaysir
Emile F
EF
D000328:Adult; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D013601:T-Lymphocytes / Q000166:cytology*