Evaluation of the protective effects of Chinese herbs against biomolecule damage induced by peroxynitrite.
This work evaluates the protective effects of Chinese herbs against ONOO(-)-induced biomolecule damage. Thirty-two Chinese traditional herbs were preliminarily screened for their ONOO(-)-scavenging activity by the fluorometric method. The potency of scavenging activity was in the following order: Apis cerana Fabricius (Propolis) > Rosmarinus officinalis L (Rosemary) > Pseudolarix amabilis (Nelson) Rehd. (Pine Bark PE) > Echinacea Moenck. (Echinacea); the remaining twenty-eight herbs performed unsatisfactorily for their scavenging activity. The four extracts with high ONOO(-) scavenging activity were selected for further characterization of their inhibiting effects on DNA single breaks, tyrosine nitration and LDL oxidation. A further study of the origin of this scavenging activity on the major active components showed cichoric acid and rosemary acid to both have strong ability to scavenge ONOO(-). These scavengers might be developed as therapeutic drugs for preventing ONOO(-)-involved diseases.
Luo
Yunjing
Y
College of Life Science and Bioengineering, Beijing University of Technology, China. luoyj@bjut.edu.cn
Pan
Jinglin
J
Pan
Yanshu
Y
Han
Zhiguo
Z
Zhong
Rugang
R
D053960:DNA Breaks / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019793:Fluorescein / Q000378:metabolism; D005470:Fluorometry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D030421:Peroxynitrous Acid / Q000633:toxicity*; D014443:Tyrosine / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D030421:Peroxynitrous Acid; D014443:Tyrosine; D019793:Fluorescein