Dark proteins disturb multichromophore coupling in tetrameric fluorescent proteins.
DsRed is representative of the tetrameric reef coral fluorescent proteins that constitute particularly interesting coupled multichromophoric systems. Either a green emitting or a red emitting chromophore can form within each of the monomers of the protein tetramer. Within the tetramers the chromophores are thought to be efficiently fluorescence resonance energy transfer (FRET) coupled. We have used spectrally resolved room temperature single molecule spectroscopy to address the issue of FRET and the role of dark proteins within single protein tetramers of DsRed and its variants DsRed2, DsRed_N42H and AG4. Our results show that for the majority of the tetramers the different chromophores are indeed effectively coupled. However, in a fraction of the tetramers that is characteristic for each DsRed variant analyzed, we observe a lack of effective FRET coupling. For these tetramers we invoke the existence of dark proteins lacking a functional chromophore that interrupt the energy transfer chain within the multichromophoric system. We show that these species lead to donor dequenching that strongly influences the bulk emission spectra.
Blum
Christian
C
Nanobiophysics, MESA+Institute for Nanotechnology & MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. c.blum@tnw.utwente.nl
Meixner
Alfred J
AJ
Subramaniam
Vinod
V
D003116:Color; D003624:Darkness; D031541:Fluorescence Resonance Energy Transfer; D008164:Luminescent Proteins / Q000737:chemistry*; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013696:Temperature
D008164:Luminescent Proteins; C413663:fluorescent protein 583