Efficient production of L-lactic acid from cassava powder by Lactobacillus rhamnosus.
Cassava is one of the most efficient and rich crops in terms of carbohydrate production, which is a tropical perennial plant that grows on poor or depleted soils. Microbial conversion of such a renewable raw material to useful products is an important objective in industrial biotechnology. L-Lactic acid was efficiently produced from cassava powder by a Lactobacillus rhamnosus strain CASL. The fermentation properties of cassava powder were compared with those of glucose and corn powder. The efficiencies of various fermentation strategies for L-lactic acid production from cassava powder, including simultaneous saccharification and fermentation (SSF), two-step fermentation (TSF) and simultaneous liquefaction, saccharification and fermentation (SLSF), were investigated. The high L-lactic acid concentration (175.4 g/l) was obtained using 275 g/l of cassava powder concentration (total sugar of 222.5 g/l) in SSF batch fermentation. This is the highest L-lactic acid concentration reported, from cassava source, and it provides an efficient L-lactic acid production process with cheap raw bioresources, such as cassava powder.
Wang
Limin
L
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Zhao
Bo
B
Liu
Bo
B
Yang
Chunyu
C
Yu
Bo
B
Li
Qinggang
Q
Ma
Cuiqing
C
Xu
Ping
P
Ma
Yanhe
Y
D019149:Bioreactors; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D002365:Manihot / Q000737:chemistry*; D011208:Powders
D011208:Powders; D019344:Lactic Acid; D005087:Glucan 1,4-alpha-Glucosidase