Analysis of genes, transcripts, and proteins via DNA ligation.
Analytical reactions in which short DNA strands are used in combination with DNA ligases have proven useful for measuring, decoding, and locating most classes of macromolecules. Given the need to accumulate large amounts of precise molecular information from biological systems in research and in diagnostics, ligation reactions will continue to offer valuable strategies for advanced analytical reactions. Here, we provide a basis for further development of methods by reviewing the history of analytical ligation reactions, discussing the properties of ligation reactions that render them suitable for engineering novel assays, describing a wide range of successful ligase-based assays, and briefly considering future directions.
Conze
Tim
T
Department of Genetics and Pathology, The Rudbeck Lab, Uppsala University, Uppsala, Sweden.
Shetye
Alysha
A
Tanaka
Yuki
Y
Gu
Jijuan
J
Larsson
Chatarina
C
Göransson
Jenny
J
Tavoosidana
Gholamreza
G
Söderberg
Ola
O
Nilsson
Mats
M
Landegren
Ulf
U
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011088:DNA Ligases / Q000737:chemistry / Q000378:metabolism*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D008967:Molecular Biology / Q000266:history / Q000379:methods*
D004247:DNA; D011088:DNA Ligases