title: Label-free and highly sensitive optical imaging of detailed microcirculation within meninges and cortex in mice with the cranium left intact.
abstract: We demonstrate for the first time that the detailed blood flow distribution within intracranial dura mater and cortex can be visualized by an ultrahigh sensitive optical microangiography (UHS-OMAG). The study uses an UHS-OMAG system operating at 1310 nm with an imaging speed at 150 frames per second that requires approximately 10 s to complete one 3-D scan of approximately 2.5 x 2.5 mm(2). The system is sensitive to blood flow with a velocity ranging from approximately 4 microms to approximately 23 mms. We show superior performance of UHS-OMAG in providing functional images of capillary level microcirculation within meninges in mice with the cranium left intact, the results of which correlate well with the standard dural histopathology.
authors.forename: Yali
authors.initials: Y
authors.lastname: Jia
authors.forename: Lin
authors.initials: L
authors.lastname: An
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply*; D008578:Meninges / Q000098:blood supply*; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000502:physiology*; D008857:Microscopy, Interference / Q000379:methods*; D055616:Microtechnology; D012039:Regional Blood Flow / Q000502:physiology; D012680:Sensitivity and Specificity; D012886:Skull; D041623:Tomography, Optical Coherence / Q000379:methods*