Serum monocyte chemotactic protein-1 concentrations distinguish patients with ankylosing spondylitis from patients with mechanical low back pain.
OBJECTIVE
This study aimed to identify potential blood-derived biomarkers distinguishing patients with ankylosing spondylitis from those with mechanical low back pain.


METHODS
Serum and synovial fluid samples from our cohorts were assayed by using enzyme-linked immunosorbent assay for the following inflammatory biomarkers: interleukin (IL)-1α, IL-6, IL-8, IL-17, IL-23, monocyte chemotactic protein (MCP)-1, macrophage inflammatory proteins (MIP)-1α, MIP-1β, tumor necrosis factor-α (TNF-α), interferon-α (IFN-α), IFN-β, metalloproteinase (MMP-3), and bone morphogenetic protein 7 (BMP-7).


RESULTS
After screening, a panel of serum and synovial fluid samples with a series of potential biomarkers, cytokines including IL-6, IL-8, MMP-3, and MCP-1 were selected for additional testing because they exhibited higher concentrations than paired serum samples in the synovial fluid. Sera obtained from 50 patients with ankylosing spondylitis and 27 patients with mechanical low back pain were measured for these biomarkers.


CONCLUSIONS
The MCP-1 serum was identified as a biomarker candidate, distinguishing ankylosing spondylitis from mechanical low back pain with a sensitivity of 96% and a specificity of 83.3%.
Romero-Sanchez
Consuelo
C
Spondyloarthropaty Group, Division of Rheumatology, Hospital Militar Central/Universidad de la Sabana, Bogotá, Colombia.
Tsou
Hsi-Kai
HK
Jan
Ming-Shiou
MS
Wong
Ruey-Hong
RH
Chang
I-Chang
IC
Londoño
John
J
Valle-Oñate
Rafael
R
Howe
Hwee Siew
HS
Yu
David
D
Leung
Bernard P
BP
Wei
James Cheng-Chung
JC
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D018932:Chemokine CCL2 / Q000097:blood*; D015331:Cohort Studies; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D017116:Low Back Pain / Q000097:blood* / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013167:Spondylitis, Ankylosing / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D055815:Young Adult
D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2