Synthesis, DNA-binding and photocleavage of "light switch" complexes [Ru(bpy)2(pyip)]2+ and [Ru(phen)2(pyip)]2+.
Two novel Ru(II) complexes [Ru(bpy)(2)(pyip)](2+)1 and [Ru(phen)(2)(pyip)](2+)2 (bpy=2,2'-bipyridine; phen=1,10-phenanthroline; pyip=2-(pyridine-2-yl)imidazo-[4,5-f][1,10]-phenanthroline), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR, UV-Vis. The DNA-binding behaviors of both complexes were studied by spectroscopic methods and viscosity measurements. The results indicate that the two complexes can bind to CT-DNA in an intercalative mode, and also show that these two Ru(II) complexes can promote the photocleavage of pBR322 DNA. In addition, In the presence of Co(2+), the emission of DNA-[Ru(L)(2)pyip](2+) can be quenched, which exhibited the DNA "light switch" properties.
Liu
Xue-Wen
XW
College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China. liuxuewen050@sina.com
Shen
You-Ming
YM
Lu
Ji-Lin
JL
Chen
Yuan-Dao
YD
Li
Lin
L
Zhang
Da-Shun
DS
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry; D008027:Light; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry* / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry* / Q000378:metabolism
D007364:Intercalating Agents; D010618:Phenanthrolines; D012428:Ruthenium; D004247:DNA; C025205:1,10-phenanthroline