Optimized surface-enhanced Raman scattering (SERS) colloids for the characterization of microorganisms.
We report on a comparison of silver colloid preparation methods for the in situ analysis of microorganisms based on surface-enhanced Raman scattering (SERS). Different colloid sols were tested and optimized regarding their suitability as SERS substrates. The silver sols produced by a modified procedure of Leopold and Lendl gave an enhancement factor of the Raman signal in the order 10(8) for the test molecule, crystal violet. Furthermore, this SERS substrate was successfully applied for the in situ detection and identification of microorganisms immobilized on an immunoassay. The colloid preparation was carried out at room temperature and the colloids were stable for weeks. This silver nanoparticle preparation method for the label-free in situ detection of microorganisms successfully combines SERS with immunoassays. Hence, it has a great potential for the high-throughput detection of biomolecules.
Knauer
Maria
M
Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, 81377 München, Germany.
Ivleva
Natalia P
NP
Niessner
Reinhard
R
Haisch
Christoph
C
D000818:Animals; D001419:Bacteria / Q000276:immunology / Q000302:isolation & purification*; D003102:Colloids; D007118:Immunoassay; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
D003102:Colloids; D012834:Silver