title: Analysis of Leigh syndrome mutations in the yeast SURF1 homolog reveals a new member of the cytochrome oxidase assembly factor family.
abstract: Three missense SURF1 mutations identified in patients with Leigh syndrome (LS) were evaluated in the yeast homolog Shy1 protein. Introduction of two of the Leigh mutations, F(249)T and Y(344)D, in Shy1 failed to significantly attenuate the function of Shy1 in cytochrome c oxidase (CcO) biogenesis as seen with the human mutations. In contrast, a G(137)E substitution in Shy1 results in a nonfunctional protein conferring a CcO deficiency. The G(137)E Shy1 mutant phenocopied shy1Delta cells in impaired Cox1 hemylation and low mitochondrial copper. A genetic screen for allele-specific suppressors of the G(137)E Shy1 mutant revealed Coa2, Cox10, and a novel factor designated Coa4. Coa2 and Cox10 are previously characterized CcO assembly factors. Coa4 is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space and associated with the inner membrane. Cells lacking Coa4 are depressed in CcO activity but show no impairment in Cox1 maturation or formation of the Shy1-stabilized Cox1 assembly intermediate. To glean insights into the functional role of Coa4 in CcO biogenesis, an unbiased suppressor screen of coa4Delta cells was conducted. Respiratory function of coa4Delta cells was restored by the overexpression of CYC1 encoding cytochrome c. Cyc1 is known to be important at an ill-defined step in the assembly and/or stability of CcO. This new link to Coa4 may begin to further elucidate the role of Cyc1 in CcO biogenesis.
authors.affiliation: University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, Utah 84132, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Bestwick
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Jeong
authors.forename: Oleh
authors.initials: O
authors.lastname: Khalimonchuk
authors.forename: Hyung
authors.initials: H
authors.lastname: Kim
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Winge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019069:Cell Respiration / Q000502:physiology; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007888:Leigh Disease / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D014176:Protein Biosynthesis; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: C530854:Coa2 protein, S cerevisiae; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D021122:Protein Subunits; C106291:SHY1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C092928:Surf-1 protein; D003576:Electron Transport Complex IV