title: Separation of an aqueous extract Inonotus obliquus (Chaga). A novel look at the efficiency of its influence on proliferation of A549 human lung carcinoma cells.
abstract: Aqueous extract of Inonotus obliquus was hydrolyzed in dilute hydrochloric acid. The products were extracted applying organic solvents, and separated chromatographically on a silica gel-packed column. Eluted fractions were analyzed by means of GC-MS. The presence of hydrocarbons, alcohols, phenols and various carbonyl compounds in analyzed fractions has been detected and quantified. Preliminarily experiments on the influence of certain separated samples on the proliferation of A549 human lung carcinoma cells were performed. Therefore, we hypothesize that the major antiproliferative effects are related to the presence of benzaldehyde, which is a benzyl alcohol metabolite formed in situ in the cells culture with the yield moderated by the presence of trace amounts of "high molecular mass compounds".
authors.affiliation: Faculty of Chemistry, Rzeszów University of Technology, A1. Powstańców Warszawy 6, 35-011 Rzeszów, Poland. mowi@prz.edu.pl
authors.forename: Witold
authors.initials: W
authors.lastname: Mazurkiewicz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rydel
authors.forename: Dariusz
authors.initials: D
authors.lastname: Pogocki
authors.forename: Marta Kinga
authors.initials: MK
authors.lastname: Lemieszek
authors.forename: Ewa
authors.initials: E
authors.lastname: Langner
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D008970:Molecular Weight; D011124:Polyporaceae / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic