title: In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy.
abstract: We first report on the implementation of a novel narrow-band image-guided Raman endoscopy technique for in vivo diagnosis of gastric dysplasia. High-quality in vivo Raman spectra can be acquired from normal and dysplastic gastric mucosal tissue within 0.5 sec under narrow-band image (NBI) guidance at gastroscopy. Significant differences are observed in in vivo Raman spectra between normal (n=54) and dysplastic (n=18) gastric tissue from 30 gastric patients, particularly in the spectral ranges of 825 to 950, 1000 to 1100, 1250 to 1500, and 1600 to 1800 cm(-1), which primarily contain signals related to proteins, nucleic acids, and lipids. The multivariate analysis [i.e., principal components analysis (PCA) and linear discriminant analysis (LDA)], together with the leave-one tissue site-out, cross validation on in vivo gastric Raman spectra yields a diagnostic sensitivity of 94.4% (1718) and specificity of 96.3% (5254) for distinction of gastric dysplastic tissue. This study suggests that narrowband image-guided Raman endoscopy associated with PCA-LDA diagnostic algorithms has potential for the noninvasive, in vivo early diagnosis and detection of gastric precancer during clinical gastroscopic examination.
authors.affiliation: National University of Singapore, Department of Bioengineering, Faculty of Engineering, Optical Bioimaging Laboratory, Singapore 117576. biehzw@nus.edu.sg
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Huang
authors.forename: Mads Sylvest
authors.initials: MS
authors.lastname: Bergholt
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Kan
authors.initials: K
authors.lastname: Lin
authors.forename: Khek Yu
authors.initials: KY
authors.lastname: Ho
authors.forename: Ming
authors.initials: M
authors.lastname: Teh
authors.forename: Khay Guan
authors.initials: KG
authors.lastname: Yeoh
mesh_terms: D000368:Aged; D000465:Algorithms; D016022:Case-Control Studies; D016002:Discriminant Analysis; D005260:Female; D005773:Gastroscopy / Q000379:methods*; D006801:Humans; D008297:Male; D019060:Minimally Invasive Surgical Procedures / Q000379:methods; D015999:Multivariate Analysis; D011230:Precancerous Conditions / Q000737:chemistry / Q000175:diagnosis*; D025341:Principal Component Analysis; D012372:ROC Curve; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013272:Stomach Diseases / Q000175:diagnosis*; D025321:Surgery, Computer-Assisted / Q000379:methods*