[A direct accelerated method for detection of a whooping cough pathogen].
The developed direct method for the laboratory diagnosis of pertussis, which is based on isothermal amplification technologies, has a high (100%) specificity and sensitivity (102 m.cl.), can detect the pathogen of the disease just in the clinical sample from a patient within 7-8 hours after start of the study. The clinical trials conducted at Infectious Diseases Hospital One (Moscow) on examination of 103 patients (63 patients with the clinical diagnosis of pertussis and 40 with other respiratory tract diseases) provided evidence its high specificity and diagnostic efficiency as compared with a bacteriological test, the efficiency in different clinical types of the disease and during examinations of patients in different periods after the onset of the disease, as well as during examinations of patients with suspected pertussis and pertussis-like diseases.
Borisova
O Iu
OIu
Petrova
M S
MS
Gadua
N T
NT
Skachkova
V G
VG
Savinkova
V S
VS
Popova
O P
OP
Mazurova
I K
IK
Aleshkin
V A
VA
D000293:Adolescent; D001431:Bacteriological Techniques; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques; D021141:Nucleic Acid Amplification Techniques; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000382:microbiology*
D004269:DNA, Bacterial