[Expression of MMP-2 during differentiation of vascular endothelial cell from bone marrow mesenchymal stem cell in porcine].
OBJECTIVE
To investigate the expression of MMP-2 in porcine bone marrow mesenchymal stem cells (MSCs) in the process of differentiation into vascular endothelial cells in vitro.


METHODS
Porcine MSCs were isolated from bone marrow and grown in vitro. At 3-, 7-day after VEGF induction, immunohistochemical and flow cytometrical examinations were carried out to detect the staining of FVIII. The expression of MMP-2 was analyzed by Western blot.


RESULTS
At 3-, 7-day after VEGF induction, the morphology of the cells gradually changed. The cells gradually showed a ball-like appearance at 3-day. They connected with adjacent cells and formed tube-like structure after 7 days. The factor VIII-positive cells could be observed at 3-day after VEGF induction. The positive cells increased and joined to neighboring FVIII-positive cells, formed tube-like structures at 7-day after VEGF treatment. MSCs maintained in control medium stained negative. FACS analysis showed that untreated MSCs were negative for FVIII. The number of FVIII positive cells was not significantly increased at 3-day after induction (3%). But at 7-day after induction, FVIII-positive cells were significantly increased (13%). The expression of MMP-2 was increased as early as 1 hour post VEGF treatment.


CONCLUSIONS
Porcine MSCs have the potential to differentiate into vascular endothelial cells in vitro and this process involves MMP-2.
Fang
Li-jun
LJ
Department of Stomatology of Shengjing Hospital, Chinese Medical University, Shenyang 110004, China. lijunfang999@sina.com
Guo
Shu
S
Wang
Qiu-xu
QX
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D042783:Endothelial Cells; D020778:Matrix Metalloproteinase 2; D059630:Mesenchymal Stem Cells; D013552:Swine
D020778:Matrix Metalloproteinase 2