title: A correlation between polyomavirus JC virus quantification and genotypes in renal transplantation.
abstract: OBJECTIVE
  To determine whether the John Cunningham virus (JCV) viral load and the multigenotypes in viruria are correlated with transplant patients.
  
  
  METHODS
  The urine of 60 renal transplant patients and 60 healthy controls were screened. We used quantitative real-time polymerase chain reaction and capillary electrophoresis to assess viral load and genotype respectively.
  
  
  RESULTS
  The incidence of viruria and viral load were higher in transplant patients with P = .0092 and P = .0094, respectively. The incidence of different genotype in transplant patients versus controls was 8.3% versus 13.3% for single genotype, 26.7% versus 5% for 2 genotypes, and 5% versus 0% for multigenotypes (P = .0004). The incidence of more than 2 genotypes was high in people with a high viral load and closely related with the transplant patients (P = .007).
  
  
  CONCLUSIONS
  Not only viral load but also genotypes are important as a screening parameter to understand the immune milieu of the patients to prevent subsequent complications like polyomavirus nephropathy, infection, and malignancy.
authors.affiliation: Department of General Surgery, Buddhist Dalin Tzu Chi General Hospital, 2 Min-Sheng Road, Dalin town, Chia-Yi, Taiwan. yaowen@mail.tcu.edu.tw
authors.forename: Wen-Yao
authors.initials: WY
authors.lastname: Yin
authors.forename: Ming-Chi
authors.initials: MC
authors.lastname: Lu
authors.forename: Ming-Che
authors.initials: MC
authors.lastname: Lee
authors.forename: Su-Chin
authors.initials: SC
authors.lastname: Liu
authors.forename: Teng-Yi
authors.initials: TY
authors.lastname: Lin
authors.forename: Ning-Sheng
authors.initials: NS
authors.lastname: Lai
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics / Q000652:urine*; D005260:Female; D005838:Genotype; D006801:Humans; D007165:Immunosuppression Therapy; D015994:Incidence; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D027601:Polyomavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D019562:Viral Load
chemical_list: D004279:DNA, Viral