A liquid chromatographic method for routine analysis of 5'-mononucleotides in pediatric formulas.
An RP-HPLC method for the routine determination of supplemented 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in pediatric formulas and milk products is described. Following sample dissolution, potential interferences were removed by anion-exchange SPE. Chromatographic analyses were performed using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard technique. A single-laboratory validation was performed, with recoveries of 92-101% and repeatability RSDs of 1.0-2.3%. The method was optimized for the rapid, routine analysis of nucleotide-supplemented bovine milk-based infant and follow-on formulas.
Gill
Brendon D
BD
Fonterra Co-operative Group Ltd, PO Box 7, Waitoa 3380, New Zealand. brendon.gill@fonterra.com
Indyk
Harvey E
HE
Kumar
Maureen C
MC
Sievwright
Nathan K
NK
Manley-Harris
Merilyn
M
D000249:Adenosine Monophosphate / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003568:Cytidine Monophosphate / Q000032:analysis; D006157:Guanosine Monophosphate; D041943:Infant Formula / Q000737:chemistry*; D009711:Nucleotides / Q000032:analysis*; D052616:Solid Phase Extraction; D013938:Thymidine Monophosphate / Q000032:analysis; D014542:Uridine Monophosphate
D009711:Nucleotides; D013938:Thymidine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D014542:Uridine Monophosphate; D003568:Cytidine Monophosphate