title: Genetic control of IL-1 beta production and inflammatory response by the mouse Irm1 locus.
abstract: Genome-wide linkage analysis using single nucleotide polymorphism arrays was carried out in pedigrees of mice differing in the extent of acute inflammatory response (AIRmax or AIRmin). The AIR phenotype was determined by quantifying the number of infiltrating cells in the 24-h exudate induced by Biogel P-100 s.c. injection and by ex vivo IL-1beta production by leukocytes stimulated with LPS and ATP. We mapped the major inflammatory response modulator 1 locus on chromosome 7, at the 1-logarithm of odds (LOD) confidence interval from 116.75 to 139.75 Mb, linked to the number of infiltrating cells (LOD = 3.61) through the production of IL-1beta (LOD = 9.35). Of several interesting candidate genes mapping to the inflammatory response modulator 1 locus, 28 of these were differentially expressed in the bone marrow of AIRmax and AIRmin mice. These findings represent a step toward the identification of the genes underlying this complex phenotype.
authors.affiliation: Laboratório de Imunogenética, Instituto Butantan, São Paulo, Brazil.
authors.forename: Francisca
authors.initials: F
authors.lastname: Vorraro
authors.forename: Antonella
authors.initials: A
authors.lastname: Galvan
authors.forename: Wafa Hanna Koury
authors.initials: WH
authors.lastname: Cabrera
authors.forename: Patricia Santos
authors.initials: PS
authors.lastname: Carneiro
authors.forename: Orlando Garcia
authors.initials: OG
authors.lastname: Ribeiro
authors.forename: Marcelo
authors.initials: M
authors.lastname: De Franco
authors.forename: Nancy
authors.initials: N
authors.lastname: Starobinas
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: Jensen
authors.forename: Michel
authors.initials: M
authors.lastname: Seman
authors.forename: Tommaso A
authors.initials: TA
authors.lastname: Dragani
authors.forename: Olga Célia Martinez
authors.initials: OC
authors.lastname: Ibañez
mesh_terms: D000208:Acute Disease; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D056426:Genetic Loci / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D008126:Lod Score; D008297:Male; D051379:Mice; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; D053583:Interleukin-1beta