Pseudomonas putida adhesion to goethite: studied by equilibrium adsorption, SEM, FTIR and ITC.
Equilibrium adsorption along with scanning electron microscopy (SEM), Fourier transform infrared (FTIR) and isothermal titration calorimetry (ITC) techniques were employed to investigate the adhesion of Pseudomonas putida on goethite. The adhesion isotherm revealed the high affinity of P. putida for goethite. The SEM analysis also showed a tight association between bacteria and mineral particles. Larger amounts of adhesion of bacteria on goethite were observed at pH lower than the isoelectric point (IEP) of goethite. The bacterial adhesion increased with increasing concentration of K(+). The calorimetric results demonstrated that the P. putida-goethite adhesion was an exothermic process. The adhesion enthalpy increased with increasing pH and concentrations of electrolyte. The increase of the negative enthalpy with increment of temperature indicated that the bacteria-goethite adhesion was an enthalpy-driven process. Electrostatic interactions and hydrogen bonding were considered to contribute mainly to the adhesion of bacterial adhesion on goethite. The data obtained in this study would provide valuable information for a better understanding of the mechanisms of mineral-microorganism interactions in soil and associated environments.
Rong
Xingmin
X
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
Chen
Wenli
W
Huang
Qiaoyun
Q
Cai
Peng
P
Liang
Wei
W
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002151:Calorimetry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D058085:Iron Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008903:Minerals; D011188:Potassium / Q000494:pharmacology; D016958:Pseudomonas putida / Q000502:physiology* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
D058085:Iron Compounds; D008903:Minerals; C094886:goethite; D011188:Potassium