The effects of lazaroid U-74389G in a rat sepsis model.
OBJECTIVE
To examine the protective effects of a lazaroid, 21-aminosteroid U-74389G, in a rat septic shock model.


METHODS
Male Sprague-Dawley rats (n = 60) aged 6-8 months.


METHODS
Groups were exposed to 500 cGy radiation followed by E. coli inoculation, and either placebo or lazaroid injection (10 mg/kg intraperitoneal) 5 days after irradiation.


METHODS
Hemodynamic measurements, arterial blood gases, serum lactate, total antioxidative capacity, and cytokine levels were measured at specific time intervals.


RESULTS
Treatment with the lazaroid U-74389G maintained cardiac output and mean aortic pressure. Lazaroid treatment also prevented the increase in serum lactate seen in placebo-treated rats. Cytokine serum levels in lazaroid-treated rats were not significantly different from those in placebo-treated rats at any time point.


CONCLUSIONS
Lazaroid treatment of E. coli-inoculated septic animals lessens the hemodynamic deterioration seen in sepsis.
Chang
Yun-Te
YT
Department of Emergency Medicine, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung City, Taiwan.
Wann
Shue-Ren
SR
Hsieh
Kai-Hseng
KH
Liu
Yung-Ching
YC
Chang
Ching-Hsiung
CH
Huang
Mu-Shun
MS
Huang
Chun-I
CI
Chang
Hong-Tai
HT
D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000276:immunology / Q000472:pathogenicity; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D010919:Placebos; D011281:Pregnatrienes / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000188:drug therapy* / Q000401:mortality / Q000503:physiopathology; D014965:X-Rays
D007166:Immunosuppressive Agents; D010919:Placebos; D011281:Pregnatrienes; C074965:U 74389F