Genetic control of IL-1 beta production and inflammatory response by the mouse Irm1 locus.
Genome-wide linkage analysis using single nucleotide polymorphism arrays was carried out in pedigrees of mice differing in the extent of acute inflammatory response (AIRmax or AIRmin). The AIR phenotype was determined by quantifying the number of infiltrating cells in the 24-h exudate induced by Biogel P-100 s.c. injection and by ex vivo IL-1beta production by leukocytes stimulated with LPS and ATP. We mapped the major inflammatory response modulator 1 locus on chromosome 7, at the 1-logarithm of odds (LOD) confidence interval from 116.75 to 139.75 Mb, linked to the number of infiltrating cells (LOD = 3.61) through the production of IL-1beta (LOD = 9.35). Of several interesting candidate genes mapping to the inflammatory response modulator 1 locus, 28 of these were differentially expressed in the bone marrow of AIRmax and AIRmin mice. These findings represent a step toward the identification of the genes underlying this complex phenotype.
Vorraro
Francisca
F
Laboratório de Imunogenética, Instituto Butantan, São Paulo, Brazil.
Galvan
Antonella
A
Cabrera
Wafa Hanna Koury
WH
Carneiro
Patricia Santos
PS
Ribeiro
Orlando Garcia
OG
De Franco
Marcelo
M
Starobinas
Nancy
N
Jensen
José Ricardo
JR
Seman
Michel
M
Dragani
Tommaso A
TA
Ibañez
Olga Célia Martinez
OC
D000208:Acute Disease; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D056426:Genetic Loci / Q000276:immunology*; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology*; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D008126:Lod Score; D008297:Male; D051379:Mice; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000276:immunology
D018836:Inflammation Mediators; D053583:Interleukin-1beta