[Mast cell degranulation enhances exogenous dendritic cells homing to draining lymph nodes].
OBJECTIVE
To establish a method of enhancing exogenous bone marrow derived dendritic cells (BM-DC) homing to draining lymph nodes by induction of local mast cell degranulation.


METHODS
Compound 48/80 (c48/80) was injected into C57BL/6 scapular skin to induce local mast cell degranulation. BM-DC generated from bone marrow of syngenic mice were labeled with YG-Microspheres and injected into c48/80 or normal saline treated scapular skin. Cells derived from draining lymph nodes (DLN) were pooled together 48 h after BM-DC injection and stained with conjugated anti-CD11c. The efficiency of BM-DC homing to lymph nodes was analyzed by FCM.


RESULTS
Mast cell degranulation was locally boosted by c48/80 injection and caused enhancement of the homing of exogenous BM-DC to DLN, with an increase of 67%+/-43%. The total cells in lymph nodes also increased significantly. The fold of increase was 55%+/-43%.


CONCLUSIONS
Exogenous BM-DC homing to DLN can be boosted by inducing local skin mast cell degranulation.
Ren
Shu-rong
SR
Department of Immunology, Qingdao University Medical College, Qingdao 266071, China. ren.shurong@gmail.com
Xu
Li-bin
LB
Wu
Zhi-yuan
ZY
Qu
Chun-feng
CF
Gao
Mei-hua
MH
Du
Jun
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D015550:Cell Degranulation; D002465:Cell Movement; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL