Deficiency of the chemokine receptor CXCR2 attenuates neutrophil infiltration and cortical damage following closed head injury.
The contribution of infiltrated neutrophils to secondary damage following traumatic brain injury remains controversial. Chemokines that regulate neutrophil migration by signaling through the CXCR2 receptor are markedly elevated by brain injury and are associated with the propagation of secondary damage. This study thus investigated the function of CXCR2 in posttraumatic inflammation and secondary degeneration by examining Cxcr2-deficient (Cxcr2(-/-)) mice over 14 days following closed head injury (CHI). We demonstrate a significant attenuation of neutrophil infiltration in Cxcr2(-/-) mice at 12 hours and 7 days after CHI, despite increased levels of CXC neutrophil-attracting chemokines in the lesioned cortex. This coincides with reduced tissue damage, neuronal loss, and cell death in Cxcr2(-/-) mice compared to wild-type controls, with heterozygotes showing intermediate responses. In contrast, blood-brain barrier permeability and functional recovery did not appear to be affected by Cxcr2 deletion. This study highlights the deleterious contribution of neutrophils to posttraumatic neurodegeneration and demonstrates the importance of CXC chemokine signaling in this process. Therefore, CXCR2 antagonistic therapeutics currently in development for other inflammatory conditions may also be of benefit in posttraumatic neuroinflammation.
Semple
Bridgette D
BD
National Trauma Research Institute, The Alfred Hospital, Melbourne, Victoria, Australia. Bridgette.Semple@monash.edu
Bye
Nicole
N
Ziebell
Jenna M
JM
Morganti-Kossmann
M Cristina
MC
D000367:Age Factors; D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology; D016923:Cell Death; D002540:Cerebral Cortex / Q000276:immunology* / Q000473:pathology; D018925:Chemokines / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016489:Head Injuries, Closed / Q000276:immunology* / Q000473:pathology; D006579:Heterozygote; D006720:Homozygote; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D020556:Neutrophil Infiltration / Q000276:immunology*; D023063:Receptors, Interleukin-8B / Q000172:deficiency* / Q000235:genetics; D020127:Recovery of Function
D018925:Chemokines; D016207:Cytokines; D023063:Receptors, Interleukin-8B