title: Determination of hydrogen cyanide in cigarette mainstream smoke by LC/MS/MS.
abstract: An LC/MS/MS method is presented for the determination of hydrogen cyanide in cigarette mainstream smoke. Cyanide is derivatized with 2,3'-naphthalenedicarboxaldehyde and taurine to form a benzo[f]isoindole derivative, which is then analyzed by LC/MS/MS. Isotopic KCN (K13C15N) was used as an internal standard. The regression equation was linear within the range 2.4-331 ng/mL for cyanide with a correlation coefficient > 0.999. The LOD was calculated as 4.1 ng/cigarette. The influence of the sodium hydroxide trapping solution concentration on the results is discussed. A 1 M solution showed the best results in terms of sample stability and trapping efficiency. The method proved to be robust, reliable, and more selective than current methods, making it a logical choice for determination of total cyanide in cigarette smoke.
authors.affiliation: Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland. nicolas.mottier@pmintl.com
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mottier
authors.forename: Florent
authors.initials: F
authors.lastname: Jeanneret
authors.forename: Michel
authors.initials: M
authors.lastname: Rotach
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006856:Hydrogen Cyanide / Q000032:analysis*; D057230:Limit of Detection; D012906:Smoke / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014026:Nicotiana
chemical_list: D012906:Smoke; D006856:Hydrogen Cyanide