title: Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of beta-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L(-1) initial CMC, approximately 3.45 g L(-1) ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g(-1) from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
authors.affiliation: School of Life Science and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Jeong-eun
authors.initials: JE
authors.lastname: Hyeon
authors.forename: Kyung-Ok
authors.initials: KO
authors.lastname: Yu
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Suh
authors.forename: Young-Woong
authors.initials: YW
authors.lastname: Suh
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
authors.forename: Sung Ok
authors.initials: SO
authors.lastname: Han
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D044603:Cellulosomes / Q000235:genetics / Q000378:metabolism*; D046974:Clostridium cellulovorans / Q000201:enzymology* / Q000235:genetics; D048013:Acetivibrio thermocellus / Q000201:enzymology* / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000431:Ethanol; D002482:Cellulose