title: Molecular typing of avian pathogenic Escherichia coli O78 strains in Japan by using multilocus sequence typing and pulsed-field gel electrophoresis.
abstract: Avian pathogenic Escherichia coli (APEC) serogroup O78 isolates in Japan were characterized using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Thirty-four of the serotype O78 isolates were clustered into 11 PFGE types with 80% similarity and were classified into 5 sequence types (STs), ST23, ST117, ST155, ST369 and 1 novel ST (ST1645). The most dominant ST was ST23 (54.3%). Fluoroquinolone-resistant strains belonged to ST23 (9 strains), ST155 (3 strains) and ST117 (1 strain). The combined findings of MLST and PFGE suggested that 1 genotype belonging to ST23 was the genotype specific to fluoroquinolone-resistant strains. These results indicate that the combination of MLST and PFGE is useful for conducting epidemiological studies on APEC O78 strains.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan. ozawa@nval.maff.go.jp
authors.forename: Manao
authors.initials: M
authors.lastname: Ozawa
authors.forename: Kotaro
authors.initials: K
authors.lastname: Baba
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002645:Chickens; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000235:genetics* / Q000662:veterinary; D024841:Fluoroquinolones / Q000627:therapeutic use; D006801:Humans; D058885:Multilocus Sequence Typing; D011201:Poultry Diseases / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific