Colorimetric pH measurement of animal cell culture media.
Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.
Jang
Juno
J
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Anamdong 5-Ga, Seongbuk-Gu, Seoul 136-701, Republic of Korea.
Moon
Soo-Jin
SJ
Hong
Sung-Hwan
SH
Kim
Ik-Hwan
IH
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D003124:Colorimetry / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration
D003470:Culture Media