Protective effect of thymoquinone in experimental testicular torsion.
OBJECTIVE
To investigate the protective role of thymoquinone (TQ) on unilateral testicular ischemia-reperfusion (I/R) injury in mice.


METHODS
Experiments were performed on male C57BL/6 mice (8 weeks old, 20-25 g). The animals were divided into 3 groups including 6 mice in each group: control (sham), torsion/detorsion (TD) and TD+TQ. Mice, except the sham-operated group, were subjected to left unilateral torsion (720° rotation in the clockwise direction). The experiments were finished after sham operation time for controls, 120 min torsion and 240 min detorsion for the other groups. In the TD+TQ group 10 mg TQ was injected intraperitoneally 30 min before detorsion.


RESULTS
In the TD group total oxidative stress (TOS), oxidative stress index (OSI) and malondialdehyde (MDA) levels were higher than in the controls. TQ treatment decreased MDA, TOS and OSI values, but did not affect the total antioxidant capacity and myeloperoxidase activity in the TD+TQ group. Upon histological examination, mice in the TD group displayed moderate-to-severe disruption of the seminiferous epithelium. Treatment with TQ resulted in significantly reduced histological damage associated with I/R injury.


CONCLUSIONS
Our results suggested that TQ treatment may have a protective effect on testicular I/R injury.
Gökçe
Ahmet
A
Department of Urology, Mustafa Kemal University, Tayfur Ata Sökmen Medical School, Antakya, Turkey. aagokce@yahoo.com
Oktar
Suleyman
S
Koc
Ahmet
A
Gonenci
Ramazan
R
Yalcinkaya
Fatih
F
Yonden
Zafer
Z
Duru
Mehmet
M
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D000975:Antioxidants; D016227:Benzoquinones; D008315:Malondialdehyde; D009195:Peroxidase; C003466:thymoquinone