Chromatographic separation and tandem MS identification of active peptides in potato protein hydrolysate that inhibit autoxidation of soybean oil-in-water emulsions.
A previously developed antioxidative potato protein hydrolysate was fractionated using gel filtration. The efficacy of different fractions for inhibiting lipid oxidation in soybean oil-in-water emulsions, neutralizing 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+*)) radicals, and chelating prooxidative metal ions was investigated. Low-molecular-weight fraction Peak 3 (709 Da) exhibited the strongest antioxidant activity and radical scavenging activity. Active peptides based on the ABTS(+*) scavenging assay were isolated by RP-HPLC and purified by UPLC. The amino acid sequence determination by MS/MS identified eight prominent peptides in the antioxidative Peak 3 fraction, of which Thr-Tyr, Tyr-Phe-Glu, Tyr-Ser-Thr-Ala, and Asn-Tyr-Lys-Gln-Met matched the sequence of papatin.
Cheng
Yu
Y
State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Chen
Jie
J
Xiong
Youling L
YL
D000595:Amino Acid Sequence; D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004655:Emulsions / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification*; D010940:Plant Proteins / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D004655:Emulsions; D010455:Peptides; D010940:Plant Proteins; D013024:Soybean Oil