Identification of a new HIV type 1 circulating BF intersubtype recombinant form (CRF47_BF) in Spain.
We report the identification of a new HIV-1 circulating recombinant form (CRF47_BF) derived from subtypes B and F. It was initially identified in protease-reverse transcriptase sequences from nine individuals from three separate regions of Spain who acquired HIV-1 infection via sexual contact. All nine sequences formed a strongly supported phylogenetic cluster, branching apart from all known CRFs, and in bootscan analyses were BF mosaics with two coincident breakpoints. Two epidemiologically unlinked viruses were sequenced in near full-length genomes, which exhibited identical mosaic structures, with 16 intersubtype breakpoints in a genome predominantly of subtype B. Subtype F segments of the new CRF failed to cluster with any of the near full-length genome subtype F sequences available in public databases. Recent dates of HIV-1 diagnoses and short genetic distances suggest a recent origin of this CRF. This is the tenth reported CRF_BF, the first apparently having originated outside of South America.
Fernández-García
Aurora
A
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Pérez-Alvarez
Lucía
L
Cuevas
María Teresa
MT
Delgado
Elena
E
Muñoz-Nieto
Mercedes
M
Cilla
Gustavo
G
Iribarren
José Antonio
JA
Pinilla
Milagros
M
Ocampo
Antonio
A
Miralles
Celia
C
Pérez-Castro
Sonia
S
González-Galeano
María
M
de Castro
Rafael Ojea
RO
Trigo
Matilde
M
García
Valentina
V
Sánchez
Ana M
AM
Thomson
Michael M
MM
D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain
C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1