title: Molecular analysis of carbohydrate-antibody interactions: case study using a Bacillus anthracis tetrasaccharide.
abstract: The process for selecting potent and effective carbohydrate antigens is not well-established. A combination of synthetic glycan microarray screening, surface plasmon resonance analysis, and saturation transfer difference NMR spectroscopy was used to dissect the antibody-binding surface of a carbohydrate antigen, revealing crucial binding elements with atomic-level detail. This analysis takes the first step toward uncovering the rules for structure-based design of carbohydrate antigens.
authors.affiliation: Department of Biomolecular Systems, Max-Planck Institute for Colloids and Interfaces, 14476 Potsdam, Germany.
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Oberli
authors.forename: Marco
authors.initials: M
authors.lastname: Tamborrini
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Tsai
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Werz
authors.forename: Tim
authors.initials: T
authors.lastname: Horlacher
authors.forename: Alexander
authors.initials: A
authors.lastname: Adibekian
authors.forename: Dominik
authors.initials: D
authors.lastname: Gauss
authors.forename: Heiko M
authors.initials: HM
authors.lastname: Möller
authors.forename: Gerd
authors.initials: G
authors.lastname: Pluschke
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D001408:Bacillus anthracis / Q000737:chemistry / Q000276:immunology*; D001666:Binding Sites, Antibody; D018604:Epitope Mapping / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D046228:Microarray Analysis / Q000379:methods; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D000906:Antibodies; D009844:Oligosaccharides