Development of molecular tools for characterization and genetic diversity analysis in Tunisian fig (Ficus carica) cultivars.
Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources.
Chatti
Khaled
K
Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092, El Manar, Tunis, Tunisia.
Baraket
Ghada
G
Ben Abdelkrim
Ahmed
A
Saddoud
Olfa
O
Mars
Messaoud
M
Trifi
Mokhtar
M
Salhi Hannachi
Amel
A
D030681:Ficus / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005787:Gene Frequency; D005788:Gene Pool; D005819:Genetic Markers / Q000235:genetics; D005821:Genetic Techniques; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers