title: Therapeutic removal of amyloid deposits in cutaneous amyloidosis by localised intra-lesional injections of anti-amyloid antibodies.
abstract: In the skin, amyloidosis can be found with or without systemic disease. Primary cutaneous amyloidosis defines those amyloidoses restricted to the skin without involvement of other systems. Here, we used conformation-specific antibodies to characterise both fibrillar and oligomeric amyloid aggregates in the skin from patients with cutaneous amyloidosis. Localised cutaneous amyloidosis with different morphology was reproduced in mice by intra-dermal (i.d.) and subdermal administration of amyloid-enhancing factor. Moreover, we demonstrated that conformational antibodies were effective in clearing amyloid deposits caused by localised intra-lesional injections without the necessity of an immune response. Given the accessibility and amyloid localization in this disease, direct i.d. injections of conformational antibodies could be a convenient and direct method for treatment.
authors.affiliation: Department of Neurology, University of Texas Medical Branch, George and Cynthia Mitchell Center for Neurodegenerative Diseases, Galveston, TX 77555, USA.
authors.forename: Audra L
authors.initials: AL
authors.lastname: Clos
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Lasagna-Reeves
authors.forename: Richard
authors.initials: R
authors.lastname: Wagner
authors.forename: Brent
authors.initials: B
authors.lastname: Kelly
authors.forename: George R
authors.initials: GR
authors.lastname: Jackson
authors.forename: Rakez
authors.initials: R
authors.lastname: Kayed
mesh_terms: D000682:Amyloid / Q000276:immunology*; D000686:Amyloidosis / Q000276:immunology / Q000473:pathology / Q000628:therapy; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods*; D007271:Injections, Intradermal; D015552:Injections, Intralesional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology / Q000628:therapy
chemical_list: D000682:Amyloid; D000906:Antibodies