Genotoxic assessment in peripheral blood lymphocytes of post-polio individuals using sister chromatid exchange analysis and micronucleus assay.
Environmental pollution is a complex issue because of the diversity of anthropogenic agents, both chemical and physical, that have been detected and catalogued. The consequences to biota from exposure to genotoxic agents present an additional problem because of the potential for these agents to produce adverse change at the cellular and organism levels. Past studies in virus have focused on structural damage to the DNA of environmental species that may occur after exposure to genotoxic agents and the use of this information to document exposure and to monitor remediation. In an effort to predict effects at the population, community and ecosystem levels, in the present study, we attempt to characterize damage occurring through genotoxic agents like 5-bromo-2-deoxyuridine, BrdU, using sister chromatid exchange technique and the formation of micronuclei (MN) in the peripheral lymphocytes of the post-polio syndrome sequelae affected by poliovirus. Analysis of structural chromosomal aberrations (CAs) and involvement of the specific chromosome break were pursued in this study. They revealed a significantly higher incidence of CAs (chromatid and chromosome breaks) in patients compared with controls, where the specific chromosome break has emerged as specific. Also, the maximum numbers of breaks were found to be in chromosome 1 at the position 1p36.1. The results also suggest a correlation between CAs and content of MN.
Bhattacharya
Saurabh Kumar
SK
Division of Biomolecules & Genetics, School of Biosciences & Technology, VIT University, Vellore, Tamilnadu, India. saurabhkumar@vit.ac.in
Saraswathy
Radha
R
Sivakumar
E
E
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002842:Chromatids; D019457:Chromosome Breakage; D004249:DNA Damage; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000473:pathology* / Q000502:physiology; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests / Q000379:methods; D008875:Middle Aged; D016262:Postpoliomyelitis Syndrome / Q000097:blood / Q000235:genetics*; D012854:Sister Chromatid Exchange; D055815:Young Adult