A simple in situ cell-based sumoylation assay with potential application to drug screening.
We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.
Muramatsu
Miyuki
M
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University.
Uwada
Junsuke
J
Matsumoto
Naoyuki
N
Saitoh
Hisato
H
D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D025842:SUMO-1 Protein / Q000378:metabolism*
D025842:SUMO-1 Protein