title: Mutagenic safety and fatty liver improvement of nanonized black soybeans in senescence-accelerated prone-8 mice.
abstract: Nanotechnology, as a new enabling technology, has the potential to revolutionize food systems. However, much attention has been focused on nanoparticle foods due to their potential physiological properties. This study was aimed to evaluate the mutagenic safety and fatty liver improvement of black soybean in senescence-accelerated mice (SAMP8). The mutagenic activity of black soybeans was investigated using the Ames test (Salmonella Typhimurium TA98, 100, 102, and 1535). Furthermore, senescence-accelerated prone-8 mice (SAMP8) have been reported to display spontaneous fatty liver. Male SAMP8 mice were divided into control and supplemented with 10% micronized or nanonized black soybeans diet and fed for 12 wk. The results revealed that the Ames test of micronized and nanonized black soybeans exhibited no mutagenicity. Administration of black soybeans to mice showed no effects on food intake and body and organ weights. The nanonized black soybean group had a lower degree of spontaneous fatty liver, alanine aminotransferase, and thiobarbituric acid-reactive substance concentrations, and had enhanced superoxide dismutase, catalase, and glutathione peroxidase activities of livers when compared with the SAMP8 control and micronized black soybean groups. The mice fed with black soybeans had significantly lower triglyceride concentrations than the SAMP8 control group. The results of this study suggest that nanonized black soybeans have no side effects and, moreover, may minimize liver lesions in SAMP8 mice.
authors.affiliation: Graduate Inst. of Veterinary Pathobiology, Natl. Chung Hsing Univ., Taichung, Taiwan.
authors.forename: J-W
authors.initials: JW
authors.lastname: Liao
authors.forename: L-Z
authors.initials: LZ
authors.lastname: Hong
authors.forename: M-F
authors.initials: MF
authors.lastname: Wang
authors.forename: S-C
authors.initials: SC
authors.lastname: Tsai
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Lin
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chan
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D019588:Aging, Premature / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004032:Diet / Q000379:methods; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000178:diet therapy* / Q000378:metabolism*; D005524:Food Technology / Q000379:methods*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity; D049329:Nanostructures / Q000737:chemistry / Q000633:toxicity; D036103:Nanotechnology / Q000379:methods*; D008517:Phytotherapy / Q000009:adverse effects / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D013025:Glycine max / Q000737:chemistry / Q000633:toxicity*
chemical_list: D009153:Mutagens; D010936:Plant Extracts