Immobilization of iron oxide magnetic nanoparticles for enhancement of vessel wall magnetic resonance imaging--an ex vivo feasibility study.
Emerging data supports a role for negative wall remodeling in the failure of vascular interventions such as vein grafts, yet clinicians/researchers currently lack the ability to temporally/efficiently investigate adventitial surface topography/total vascular wall anatomy in vivo. We established a strategy of immobilizing commercially available iron oxide magnetic nanoparticles (Fe-NPs) onto the surface of human vein conduits to facilitate high-throughput total vascular wall demarcation with magnetic resonance (MR). Binding of activated Fe-NPs to amine groups on the surface of the veins induced a thin layer of negative contrast that differentiated the adventitia from surrounding saline signal in all MR images, enabling delineation of total wall anatomy; this was not possible in simultaneously imaged unlabeled control veins. Under the conditions of this ex vivo experiment, stable covalent binding of Fe-NPs can be achieved (dose-dependent) on human vein surface for MR detection, suggesting a potential strategy for enhancing the ability of MRI to investigate total wall adaptation and remodeling in vein graft failure.
Nguyen
Binh Thai
BT
Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard-MIT Division of Heath Sciences &Technology, 65 Landsdowne Street, Cambridge, MA 02139, USA.
Vemula
Praveen Kumar
PK
Mitsouras
Dimitrios
D
Yu
Peng
P
Tao
Ming
M
Campagna
Christina
C
Mulkern
Robert V
RV
Rybicki
Frank J
FJ
Karp
Jeffrey M
JM
Ozaki
C Keith
CK
D001665:Binding Sites; D001808:Blood Vessels / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
D005290:Ferric Compounds; C000499:ferric oxide