title: Electron-microscopical localization of gelsolin in various crustacean muscles.
abstract: Gelsolin was localized by immunoelectron microscopy in fast and slow cross-striated muscles of the lobster Homarus americanus. When ultrathin sections of the muscles were labelled with anti-gelsolin and a gold-conjugated second antibody, 90% of all gold particles in the myoplasm were detected on myofibrils, preferentially in the I-band and AI-region of the sarcomeres. Both the region of the H-zone (lacking thin filaments) and the Z-disc contained no or little gold label. Under physiological conditions, a close association of gelsolin with the thin filaments was observed for both muscle types. The preferential localization of particles in the I- and AI-region indicated that gelsolin was distributed randomly over the whole length of the thin filaments. Preincubation of muscle strips with Ringer solution containing 0.5 mM EGTA resulted in a significantly different distribution pattern; gold particles were now localized preferentially in the cell periphery close to the sarcolemma, with significantly decreased abundance in the centre of the cell. Compared with the muscle under physiological conditions, the number of gold particles over sarcomeric structures was significantly reduced. Thus, binding of gelsolin to the thin filaments is apparently reversible in vivo and depends on the presence of calcium ions. We assume a functional role for gelsolin in the actin turnover processes in invertebrate muscle systems.
authors.affiliation: Institute of Anatomy and Cell Biology, University of Freiburg, Albertstrasse 23, 79104, Freiburg, Germany. andreas.unger72@gmail.com
authors.forename: Andreas
authors.initials: A
authors.lastname: Unger
authors.forename: Horst
authors.initials: H
authors.lastname: Hinssen
mesh_terms: D000199:Actins / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D018260:Gelsolin / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009210:Myofibrils / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008121:Nephropidae / Q000033:anatomy & histology / Q000737:chemistry* / Q000648:ultrastructure; D012518:Sarcomeres / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000199:Actins; D018260:Gelsolin; D002118:Calcium