title: Mosquitoes infected with dengue viruses in Brazil.
abstract: Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus.
authors.affiliation: Institute of Biomedical Sciences of the University of Sao Paulo, Sao Paulo, Brazil.
authors.forename: Mario L G
authors.initials: ML
authors.lastname: de Figueiredo
authors.forename: Almério
authors.initials: A
authors.lastname: de C Gomes
authors.forename: Alberto A
authors.initials: AA
authors.lastname: Amarilla
authors.forename: André
authors.initials: A
authors.lastname: de S Leandro
authors.forename: Agnaldo
authors.initials: A
authors.lastname: de S Orrico
authors.forename: Renato F
authors.initials: RF
authors.lastname: de Araujo
authors.forename: Jesuína
authors.initials: J
authors.lastname: do S M Castro
authors.forename: Edison L
authors.initials: EL
authors.lastname: Durigon
authors.forename: Victor H
authors.initials: VH
authors.lastname: Aquino
authors.forename: Luiz T M
authors.initials: LT
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D001938:Brazil; D009033:Culicidae / Q000821:virology*; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny