Sal k 4, a new allergen of Salsola kali, is profilin: a predictive value of conserved conformational regions in cross-reactivity with other plant-derived profilins.
The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.
Assarehzadegan
Mohammad Ali
MA
Immunology Department, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Iran.
Amini
Akram
A
Sankian
Mojtaba
M
Tehrani
Mohsen
M
Jabbari
Farahzad
F
Varasteh
Abdolreza
A
D000485:Allergens; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D011058:Pollen; D051304:Profilins / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D011487:Protein Conformation; D031248:Salsola / Q000276:immunology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000276:immunology
D000485:Allergens; D010940:Plant Proteins; D051304:Profilins; D007073:Immunoglobulin E