Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion.
BACKGROUND
Malignant pleural mesothelioma (MPM) is a rare but fatal tumour. Although most MPM patients show pleural effusion at even the early stage, it is hard to diagnose as MPM at the early stage because a sensitive and reliable diagnostic marker for MPM has not been found in plasma or pleural effusion.


METHODS
In this study, we investigated whether intelectin-1 was specifically contained in MPM cells and the pleural effusion of MPM patient by immunohistochemistry, western blotting, and enzyme-linked immunosorbent assay.


RESULTS
Malignant pleural mesothelioma cell lines, but not lung adenocarcinoma cell lines, secreted intelectin-1. In immunohistochemistry, epithelioid-type MPMs, but neither pleura-invading lung adenocarcinomas nor reactive mesothelial cells near the lung adenocarcinomas, were stained with anti-intelectin antibodies. Pleural effusion of MPM patients contained a higher concentration of intelectin-1 than that of lung cancer patients.


CONCLUSIONS
These results suggest that detection of intelectin-1 may be useful for a differential diagnosis of epithelioid-type MPM in immunohistochemistry and that a high concentration of intelectin-1 in pleural effusion can be used as a new marker for clinical diagnosis of MPM.
Tsuji
S
S
Division of Cancer Therapy, Kanagawa Cancer Center Research Institute, 1-1-2 Nakao, Asahi-ku, Yokohama-shi, Kanagawa 241-0815, Japan. stsuji@gancen.asahi.yokohama.jp
Tsuura
Y
Y
Morohoshi
T
T
Shinohara
T
T
Oshita
F
F
Yamada
K
K
Kameda
Y
Y
Ohtsu
T
T
Nakamura
Y
Y
Miyagi
Y
Y
D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D058851:GPI-Linked Proteins; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008654:Mesothelioma / Q000378:metabolism*; D008875:Middle Aged; D010994:Pleura / Q000378:metabolism; D016066:Pleural Effusion, Malignant / Q000737:chemistry / Q000378:metabolism*; D010997:Pleural Neoplasms / Q000378:metabolism*
D014408:Biomarkers, Tumor; D016207:Cytokines; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; D037102:Lectins