Ether-zymolyase ascospore isolation procedure: an efficient protocol for ascospores isolation in Saccharomyces cerevisiae yeast.
Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus improving the efficacy of genetic research and the genetic selection of S. cerevisiae strains.
Bahalul
Moran
M
Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Kaneti
Galoz
G
Kashi
Yechezkel
Y
D004986:Ether / Q000494:pharmacology; D006867:Hydrolases / Q000378:metabolism*; D009172:Mycology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
D004986:Ether; C037833:zymolyase; D006867:Hydrolases