Characterization of lipid A Cronobacter sakazakii.
We have characterized the structure of lipid A in food-borne pathogen Cronobacter sakazakii BAA-894. Lipid A molecules were purified by mild acid hydrolysis, DEAE-cellulose anion exchange chromatography and preparative thin-layer chromatography. Its structure was analyzed by electrospray ionization/mass spectrometry. Two lipid A species have been found in C. sakazakii BAA-894. The majority has a structure of disaccharide of glucosamine, phosphorylated at 1- and 4'-positions, and acylated with primary 3-hydroxymyristoyl chains at 2-, 3-, 2'- and 3'-positions and secondary myristoyl residues at 2'- and 3'- positions. The other contains a secondary lauroyl chain at 2'-position in place of the myristoyl residue.
Zhang
Chan
C
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
Li
Yanyan
Y
Tao
Guanjun
G
Li
Ye
Y
Wang
Xiaoyuan
X
D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003257:Consumer Product Safety; D044083:Cronobacter sakazakii / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis*; D008050:Lipid A / Q000032:analysis / Q000737:chemistry*; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D008050:Lipid A