Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy.
We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.
Lo
Wen
W
National Taiwan University, Department of Physics and Center of Quantum Science and Engineering, Taipei 106, Taiwan.
Wang
Tsung-Jen
TJ
Chen
Wei-Liang
WL
Hsueh
Chiu-Mei
CM
Chen
Shean-Jen
SJ
Chen
Yang-Fang
YF
Chou
Hsiu-Chu
HC
Lin
Pi-Jung
PJ
Hu
Fung-Rong
FR
Dong
Chen-Yuan
CY
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000473:pathology / Q000502:physiology; D005347:Fibroblasts; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D016014:Linear Models; D033401:Microscopy, Acoustic / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D016685:Mitomycin / Q000494:pharmacology*; D018903:Photorefractive Keratectomy; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*
D016685:Mitomycin