Full-length cDNA sequences from Rhesus monkey placenta tissue: analysis and utility for comparative mapping.
BACKGROUND
Rhesus monkeys (Macaca mulatta) are widely-used as experimental animals in biomedical research and are closely related to other laboratory macaques, such as cynomolgus monkeys (Macaca fascicularis), and to humans, sharing a last common ancestor from about 25 million years ago. Although rhesus monkeys have been studied extensively under field and laboratory conditions, research has been limited by the lack of genetic resources. The present study generated placenta full-length cDNA libraries, characterized the resulting expressed sequence tags, and described their utility for comparative mapping with human RefSeq mRNA transcripts.


RESULTS
From rhesus monkey placenta full-length cDNA libraries, 2000 full-length cDNA sequences were determined and 1835 rhesus placenta cDNA sequences longer than 100 bp were collected. These sequences were annotated based on homology to human genes. Homology search against human RefSeq mRNAs revealed that our collection included the sequences of 1462 putative rhesus monkey genes. Moreover, we identified 207 genes containing exon alterations in the coding region and the untranslated region of rhesus monkey transcripts, despite the highly conserved structure of the coding regions. Approximately 10% (187) of all full-length cDNA sequences did not represent any public human RefSeq mRNAs. Intriguingly, two rhesus monkey specific exons derived from the transposable elements of AluYRa2 (SINE family) and MER11B (LTR family) were also identified.


CONCLUSIONS
The 1835 rhesus monkey placenta full-length cDNA sequences described here could expand genomic resources and information of rhesus monkeys. This increased genomic information will greatly contribute to the development of evolutionary biology and biomedical research.
Kim
Dae-Soo
DS
National Primate Research Center, Korea Research Institute of Bioscience & Biotechnology, Ochang, Chungbuk 363-883, Republic of Korea.
Huh
Jae-Won
JW
Kim
Young-Hyun
YH
Park
Sang-Je
SJ
Lee
Sang-Rae
SR
Chang
Kyu-Tae
KT
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D015723:Gene Library; D006801:Humans; D054643:INDEL Mutation; D008253:Macaca mulatta / Q000235:genetics*; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D012333:RNA, Messenger