title: The internal domain of hordeivirus movement protein TGB1 forms in vitro filamentous structures.
abstract: The 63 kDa hordeivirus movement protein TGB1 of poa semilatent virus (the PSLV TGB1 protein) forms viral ribonucleoprotein for virus transport within a plant. It was found using the dynamic laser light scattering technique that the internal domain of TGB1 protein forms in vitro high molecular weight complexes. According to results of atomic force microscopy, a part of these complexes is represented by globules of different sizes, while another part consists of extended filamentous structures. Similar properties are also characteristic of the N-terminal half of the protein and are obviously due to its internal domain moiety. The data support the hypothesis that upon viral ribonucleoprotein complex formation, the N-terminal half of the PSLV TGB1 protein plays a structural role and exhibits the ability to form multimeric filamentous structures (the ability for self-assembly).
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Makarov
authors.forename: E A
authors.initials: EA
authors.lastname: Obraztsova
authors.forename: A G
authors.initials: AG
authors.lastname: Solovyev
authors.forename: S Yu
authors.initials: SY
authors.lastname: Morozov
authors.forename: M E
authors.initials: ME
authors.lastname: Taliansky
authors.forename: I V
authors.initials: IV
authors.lastname: Yaminsky
authors.forename: N O
authors.initials: NO
authors.lastname: Kalinina
mesh_terms: D018625:Microscopy, Atomic Force; D053830:Plant Viral Movement Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010942:Plant Viruses / Q000378:metabolism; D031785:Poa / Q000821:virology; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins