title: Estimation of relatedness among non-pedigreed Yakutian cryo-bank bulls using molecular data: implications for conservation and breed management.
abstract: BACKGROUND
  Yakutian cattle, the last remaining native cattle breed in Siberia, are well adapted to the extreme sub-arctic conditions. Nowadays only ca. 1200 purebred animals are left in Yakutia. The semen of six Yakutian bulls was stored in a cryo-bank without any pedigree documentation because of the traditional free herding style of the population.
  
  
  METHODS
  To clarify the genetic relatedness between these bulls and to provide recommendations to use their semen in future conservation and breed management programs, we have analysed 30 autosomal microsatellites and mitochondrial DNA sequences in 60 individuals including the six for which semen has been stored. Four relatedness estimators were calculated. In addition, we assessed the value of the cryo-bank bulls for the preservation of genetic variation of the contemporary Yakutian cattle by calculating allelic and gene diversity estimates and mean molecular coancestries.
  
  
  RESULTS
  On the basis of microsatellite variability, including the Yakutian cryo-bank bulls increases the allelic variation in the contemporary population by 3% and in the male subpopulation by 13%. In terms of the mean molecular coancestries, they are less related to the contemporary cow population than the breeding bulls and therefore could be used to reduce inbreeding in the living population. Although 30 loci are insufficient to resolve definitely their relatedness categories, the data suggest four pairs of cryo-bank bulls as possible half-sibs.
  
  
  CONCLUSIONS
  Our results show that even relatively limited cryo-bank storage of semen can carry allelic variation through a bottleneck. We propose a breeding scheme based on the rotation of breeding females and the division of cryo-bank bulls into three groups. Thus, if molecular data (e.g. autosomal microsatellite genotypes) for the contemporary population are available and based on relatively small-scale laboratory analyses, it is possible to avoid serious mistakes in their use for breeding applications. The approach suggested here based on the use of Yakutian cryo-bank semen can be easily extended to cryo-bank materials of other animals in future breeding programs.
authors.affiliation: Biotechnology and Food Research, MTT Agrifood Research Finland, Jokioinen, Finland.
authors.forename: Ilma
authors.initials: I
authors.lastname: Tapio
authors.forename: Miika
authors.initials: M
authors.lastname: Tapio
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Ruslan
authors.initials: R
authors.lastname: Popov
authors.forename: Zoya
authors.initials: Z
authors.lastname: Ivanova
authors.forename: Juha
authors.initials: J
authors.lastname: Kantanen
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding / Q000379:methods*; D002417:Cattle / Q000235:genetics*; D003247:Conservation of Natural Resources / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D010375:Pedigree; D010802:Phylogeny; D012800:Siberia; D014015:Tissue Banks
chemical_list: D004272:DNA, Mitochondrial