[Effect of RNAi silencing of theWT1 gene on the apoptosis of human hepatocellular carcinoma cell hepG2].
OBJECTIVE
To inhibit the expression of WT1 gene in HepG2 cells by siRNA, to study the effect of WT1 on the proliferation and apoptosis.


METHODS
HepG2 cells was transfected with WT1-siRNA by liposomes. The protein expression of WT1 in HepG2 cells was detected by immunocytochemistry to detect the effect of WT1 gene silencing. Tumor cells growth was detected by MTT assay. Morphology of apoptotic cells was observed by electron microscope and fluorescence.


RESULTS
HepG2 cells was transfected with WT1-siRNA by liposomes, and the expression of WT1 were inhibited signifycantly. The transfection rate was over 70%.Silencing of WT1 by siRNA can inhibit cell proliferation and promote apoptosis.


CONCLUSIONS
Sequence-specific siRNA targeting to WT1 can inhibit WT1 gene expression significantly. The decreesed expression of WT1 gene inhibits cell proliferation and promotes apoptosis of hepG2 cells.
Huang
Xiao-qi
XQ
First Affiliated Hospital, Guangxi Traditional Chinese Medicine College, Nanning 530023, China. xiaoqchong@163.com
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D025721:WT1 Proteins / Q000235:genetics* / Q000378:metabolism
D034741:RNA, Small Interfering; D025721:WT1 Proteins