title: Rejection of transplanted hearts in patients evaluated by the component analysis of multi-wavelength NAD(P)H fluorescence lifetime spectroscopy.
abstract: Rejection of transplanted hearts remains one of the principal reasons for death of paediatric patients, but an appropriate diagnostic tool for the mild rejection in early stages is still missing. Tissue autofluorescence (AF) is one of the most versatile non-invasive tools for mapping the metabolic state in living tissues. Increasing interest in the imaging and diagnosis of living cells and tissues based on their intrinsic fluorescence rather than fluorescence labelling is closely connected to the latest developments in high-performance spectroscopy and microscopy techniques. In this contribution, we investigate individual components in spectrally- and time-resolved NAD(P)H fluorescence, revealed by linear unmixing, responsible for increased fluorescence in patients presenting mild rejection of transplanted hearts. Application of such approach has the potential to improve the diagnostics of the cardiac transplant rejection by helping currently used histological analysis.
authors.affiliation: Department of Biophotonics, International Laser Centre, Bratislava, Slovakia. dusan@ilc.sk
authors.forename: Dusan
authors.initials: D
authors.lastname: Chorvat
authors.forename: Anton
authors.initials: A
authors.lastname: Mateasik
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Nancy
authors.initials: N
authors.lastname: Poirier
authors.forename: Joaquim
authors.initials: J
authors.lastname: Miró
authors.forename: Nagib S
authors.initials: NS
authors.lastname: Dahdah
authors.forename: Alzbeta
authors.initials: A
authors.lastname: Chorvatova
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004579:Electron Transport; D006084:Graft Rejection / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D016027:Heart Transplantation / Q000009:adverse effects*; D006801:Humans; D007223:Infant; D016014:Linear Models; D057054:Molecular Imaging; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D017785:Photons; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D009243:NAD; D009249:NADP