miR-126 functions as a tumour suppressor in human gastric cancer.
MicroRNAs have emerged as important gene regulators and are recognised as key players in carcinogenesis. In the present study, we show that miR-126 was significantly down-regulated in gastric cancer tissues compared with matched normal tissues and was associated with clinicopathological features, including tumour size, lymph node metastasis, local invasion and tumour-node-metastasis (TNM) stage. Ectopic expression of miR-126 in SGC-7901 gastric cancer cells potently inhibited cell growth by inducing cell cycle arrest in G0/G1 phase, migration and invasion in vitro as well as tumorigenicity and metastasis in vivo. Mechanistically, we identified the adaptor protein Crk as a target of miR-126. Taken together, our results suggest that miR-126 may function as a tumour suppressor in gastric cancer, with Crk as a direct target.
Feng
Runhua
R
Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui Jin Er Road, Shanghai 20025, People's Republic of China.
Chen
Xuehua
X
Yu
Yingyan
Y
Su
Liping
L
Yu
Beiqin
B
Li
Jianfang
J
Cai
Qu
Q
Yan
Min
M
Liu
Bingya
B
Zhu
Zhenggang
Z
D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D050737:Proto-Oncogene Proteins c-crk / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
C485285:CRK protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D050737:Proto-Oncogene Proteins c-crk