title: Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy.
abstract: We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.
authors.affiliation: National Taiwan University, Department of Physics and Center of Quantum Science and Engineering, Taipei 106, Taiwan.
authors.forename: Wen
authors.initials: W
authors.lastname: Lo
authors.forename: Tsung-Jen
authors.initials: TJ
authors.lastname: Wang
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Chen
authors.forename: Chiu-Mei
authors.initials: CM
authors.lastname: Hsueh
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
authors.forename: Yang-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Hsiu-Chu
authors.initials: HC
authors.lastname: Chou
authors.forename: Pi-Jung
authors.initials: PJ
authors.lastname: Lin
authors.forename: Fung-Rong
authors.initials: FR
authors.lastname: Hu
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000473:pathology / Q000502:physiology; D005347:Fibroblasts; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D016014:Linear Models; D033401:Microscopy, Acoustic / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D016685:Mitomycin / Q000494:pharmacology*; D018903:Photorefractive Keratectomy; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016685:Mitomycin