title: Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate.
abstract: The aim of this study was to evaluate the effect of different concentrations of triethylene glycol dimethacrylate (TEGDMA) on the inhibition of matrix metalloproteinase 2 (MMP-2). Mouse gingival explants were cultured overnight in DMEM and the expression of secreted enzymes was analyzed by gelatin zymography in buffers containing 5 mM CaCl(2) (Tris-CaCl(2)) in 50 mM Tris-HCl buffer with the addition of TEGDMA at different concentrations (0.62%, 1.25%, 2.5%, or 5.0% (v/v)). The gelatinolytic proteinase present in the conditioned media was characterized as matrix metalloproteinase by means of specific chemical inhibition. The matrix metalloproteinases present in the conditioned media were characterized as MMP-2 by immunoprecipitation. The eletrophoretic bands were scanned and the transmittance values were analyzed. Data was plotted and submitted to linear regression to investigate MMP-2 inhibition as a function of TEGDMA concentration. Three major bands were detected in the zymographic assays. These bands were characterized as MMP-2. Zymogene (72 kDa), intermediate (66 kDa) and active forms of MMP-2 (62 kDa) were inhibited by TEGDMA in a dose-dependent way. These findings suggest that TEGDMA could inhibit MMP-2 expression even at small concentrations.
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457, Pelotas, RS, CEP: 96015-560, Brazil. rodrigo.varella@gmail.com
authors.forename: Rodrigo Varella
authors.initials: RV
authors.lastname: de Carvalho
authors.forename: Fabrício Aulo
authors.initials: FA
authors.lastname: Ogliari
authors.forename: Marcelo Rocha
authors.initials: MR
authors.lastname: Marques
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Souza
authors.forename: Cesar Liberato
authors.initials: CL
authors.lastname: Petzhold
authors.forename: Sergio Roberto Peres
authors.initials: SR
authors.lastname: Line
authors.forename: Evandro
authors.initials: E
authors.lastname: Piva
authors.forename: Adriana
authors.initials: A
authors.lastname: Etges
mesh_terms: D000818:Animals; D003188:Composite Resins / Q000008:administration & dosage / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D003764:Dental Materials / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005881:Gingiva / Q000201:enzymology*; D047468:Immunoprecipitation; D007202:Indicators and Reagents; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D012394:Rosaniline Dyes; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D003188:Composite Resins; D017077:Culture Media, Conditioned; D003764:Dental Materials; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D061965:Matrix Metalloproteinase Inhibitors; D011109:Polymethacrylic Acids; D012394:Rosaniline Dyes; D015842:Serine Proteinase Inhibitors; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D004492:Edetic Acid; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; C004692:coomassie Brilliant Blue; D005033:Ethylmaleimide