Preconcentration and determination of iron and copper in spice samples by cloud point extraction and flow injection flame atomic absorption spectrometry.
A flow injection (FI) cloud point extraction (CPE) method for the determination of iron and copper by flame atomic absorption spectrometer (FAAS) has been improved. The analytes were complexed with 3-amino-7-dimethylamino-2-methylphenazine (Neutral Red, NR) and octylphenoxypolyethoxyethanol (Triton X-114)wasadded as a surfactant. The micellar solutionwasheated above 50 degrees C and loaded through a column packed with cotton for phase separation. Then the surfactant-rich phase was eluted using 0.05 mol L(-1) H2SO4 and the analytes were determined by FAAS. Chemical and flow variables influencing the instrumental and extraction conditions were optimized. Under optimized conditions for 25 mL of preconcentrated solution, the enrichment factors were 98 and 69, the limits of detection (3s) were 0.7 and 0.3 ng mL(-1), the limits of quantification (10s) were 2.2 and 1.0 ng mL(-1) for iron and copper, respectively. The relative standard deviation (RSD) for ten replicate measurements of 10 ng mL(-1) iron and copper were 2.1% and 1.8%, respectively. The proposed method was successfully applied to determination of iron and copper in spice samples.
Sahin
Ciğdem Arpa
CA
Hacettepe University, Chemistry Department, Ankara, Turkey. carpa@hacettepe.edu.tr
Tokgöz
Ilknur
I
Bektaş
Sema
S
D011232:Chemical Precipitation; D003300:Copper / Q000032:analysis*; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D009499:Neutral Red; D017830:Octoxynol; D011092:Polyethylene Glycols; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*; D017365:Spices / Q000032:analysis*
D009499:Neutral Red; D011092:Polyethylene Glycols; D003300:Copper; D017830:Octoxynol; C010615:Nonidet P-40; D007501:Iron