title: The promise and pitfalls of sequence-based identification of plant-pathogenic fungi and oomycetes.
abstract: Sequences of selected marker loci have been widely used for the identification of specific pathogens and the development of sequence-based diagnostic methods. Although such approaches offer several advantages over traditional culture-based methods for pathogen diagnosis and identification, they have their own pitfalls. These include erroneous and incomplete data in reference databases, poor or oversimplified interpretation of search results, and problems associated with defining species boundaries. In this letter, we outline the potential benefits and drawbacks of using sequence data for identification and taxonomic deduction of plant-pathogenic fungi and oomycetes, using phytophthora as a primary example. We also discuss potential remedies for these pitfalls and address why coordinated community efforts are essential to make such remedies more efficient and robust.
authors.forename: Seogchan
authors.initials: S
authors.lastname: Kang
authors.forename: Michele A
authors.initials: MA
authors.lastname: Mansfield
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Park
authors.forename: David M
authors.initials: DM
authors.lastname: Geiser
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Ivors
authors.forename: Michael D
authors.initials: MD
authors.lastname: Coffey
authors.forename: Niklaus J
authors.initials: NJ
authors.lastname: Grünwald
authors.forename: Frank N
authors.initials: FN
authors.lastname: Martin
authors.forename: C André
authors.initials: CA
authors.lastname: Lévesque
authors.forename: Jaime E
authors.initials: JE
authors.lastname: Blair
mesh_terms: D048028:DNA, Algal; D004271:DNA, Fungal; D030561:Databases, Nucleic Acid; D005658:Fungi / Q000145:classification* / Q000235:genetics; D010838:Phytophthora / Q000145:classification* / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D048028:DNA, Algal; D004271:DNA, Fungal