title: Dietary zinc oxide affects the expression of genes associated with inflammation: Transcriptome analysis in piglets challenged with ETEC K88.
abstract: The post-weaning growth check in commercial pig production systems is often associated with gastrointestinal infection, in particular that caused by enterotoxigenic Escherichia coli (ETEC) K88. Pharmacological doses of zinc oxide (ZnO) in the post-weaning diet reduce the incidence of diarrhoea and improve piglet performance. In the present study, piglets reared indoors or outdoors and weaned onto diets with or without pharmacological levels of ZnO were orally challenged with ETEC K88. Quantitative real-time PCR was performed on RNA extracted from jejunal lamina propria and Peyer's patch samples, to compare expression of a variety of candidate genes between treatments. Candidate genes were selected from an initial microarray study using pooled RNA to identify differentially expressed genes. Dietary treatment with ZnO was associated with significant differences in the transcript abundance of several genes. Zinc supplementation was associated with a marked decrease in expression of immune response genes concerned with inflammation, and possibly related to the stage of infection. Interestingly, evidence was also obtained that a reduced level of MUC4 (a proposed ETEC K88 receptor) was associated with zinc supplementation suggesting a mechanism that might influence ETEC infection. These findings indicate that zinc oxide supplementation may reduce the level of inflammation caused by ETEC challenge.
authors.affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors.forename: Hannah R
authors.initials: HR
authors.lastname: Sargeant
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: McDowall
authors.forename: Helen M
authors.initials: HM
authors.lastname: Miller
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Shaw
mesh_terms: D000818:Animals; D004032:Diet; D054307:Enterotoxigenic Escherichia coli; D004777:Environment; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D007249:Inflammation / Q000276:immunology / Q000517:prevention & control*; D016328:NF-kappa B / Q000502:physiology; D016133:Polymerase Chain Reaction; D013552:Swine; D015034:Zinc Oxide / Q000008:administration & dosage*
chemical_list: D016328:NF-kappa B; D015034:Zinc Oxide