title: Quantification of phospholipids in infant formula and growing up milk by high-performance liquid chromatography with evaporative light scattering detector.
abstract: Phospholipids (PLs) are well known for their excellent emulsifier properties and more recently for their biological functions, such as cell signing, brain development, immune function, heart health, and cancer prevention, besides their physiological role in membrane composition. In dairy products, PLs represent 0.2-1% of milk fat. The milk PLs comprise phosphatidylcholine (PC), phosphatidylethanolamine (PE), and sphingomyelin (SPH) as the major compounds; phosphatidylinositol and phosphatidylserine are minor PLs. A new generation of dairy products claiming PL family content, such as SPH, is being produced; therefore, a validated method for quantifying PL families in dairy products is needed. In this study, an HPLC-evaporative light scattering detector method to quantify the most abundant milk PL families, i.e., PC, PE, and SPH, in infant formula and growing up milk was developed and validated.
authors.affiliation: Nestlé Product Technology Centre, Konolfingen, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Braun
authors.forename: Brigitte
authors.initials: B
authors.lastname: Flück
authors.forename: Claudia
authors.initials: C
authors.lastname: Cotting
authors.forename: Florence
authors.initials: F
authors.lastname: Monard
authors.forename: Francesca
authors.initials: F
authors.lastname: Giuffrida
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D041943:Infant Formula / Q000737:chemistry*; D008027:Light; D008892:Milk / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*; D015203:Reproducibility of Results; D012542:Scattering, Radiation
chemical_list: D010743:Phospholipids