Preoxygenation of different preservation solutions for porcine pancreas preservation.
BACKGROUND
Organ preservation quality impacts porcine islet cell isolation and transplantation success. Among several preservation methods, the two-layer method is promising, but technically demanding and fails to deliver sufficient oxygen. The use of hyperbaric oxygenation may be an easier, more effective method to supply high partial pressure of oxygen (pO(2)) for organ storage. Therefore, the aim of this study was to test the capability of preoxygenation of various preservation solutions with HBO to maintain high pO(2) levels.


METHODS
University of Wisconsin (UW), Custodiol, Perfadex, or Celsior solutions were preoxygenated in a pressure chamber. NaCl served as the control. pO(2) levels were measured at defined times. The oxygen storage capability was evaluated by leaving the storage bottles open for 2 minutes.


RESULTS
It was feasible to preoxygenate preservation solutions. The best solution to maintain high pO(2) tensions was Perfadex, followed by Celsior, and UW.


CONCLUSIONS
The greater the amount of oxygen in the preservation solution, the more oxygen can be delivered to the preserved pancreas. Further studies on the influence of preoxygenated preservation solutions on the porcine pancreas are warranted to improve organ quality, porcine islet cell isolation, and transplantation success.
Hackl
F
F
Department of Surgery, Division of Transplantation Surgery Medical University of Gray, Austria.
Stiegler
P
P
Stadlbauer
V
V
Schaffellner
S
S
Iberer
F
F
Matzi
V
V
Maier
A
A
Klemen
H
H
Smolle-Jüttner
F M
FM
Tscheliessnigg
K
K
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D002951:Citrates / Q000494:pharmacology; D004187:Disaccharides / Q000494:pharmacology; D004573:Electrolytes / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006931:Hyperbaric Oxygenation / Q000379:methods; D007328:Insulin / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects; D010313:Partial Pressure; D011887:Raffinose / Q000494:pharmacology; D013552:Swine
C088453:Celsior; D002951:Citrates; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D019188:Organ Preservation Solutions; C070005:Perfadex; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen