Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora.
Eight actinomycete strains that form bud-like spore chains were isolated from various samples collected in Japan. Phylogenetically, the isolates formed a single clade with the type strain of Actinomycetospora chiangmaiensis according to 16S rRNA gene sequence analysis. The isolates contained meso-diaminopimelic acid, d-glutamic acid and d- and l- alanine in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, phosphatidylcholine and phosphatidylethanolamine as diagnostic phospholipids, MK-8(H(4)) as the predominant isoprenoid quinone, iso-C(16 : 0) as the major cellular fatty acid and DNA G+C contents of 72-74 mol%. Actinomycetospora chiangmaiensis, the type species of the genus Actinomycetospora, was also found to contain MK-8(H(4)) predominantly in our study, although it was earlier reported to contain MK-9(H(4)) as the predominant isoprenoid quinone. On the basis of the morphological, physiological, chemotaxonomic, phylogenetic and DNA-DNA hybridization data, we concluded that the isolates can be accommodated in the genus Actinomycetospora with emendation of the description of the genus and are assigned to the following seven novel species: Actinomycetospora chibensis sp. nov. (type strain TT04-21(T)  = NBRC 103694(T)  = KACC 14256(T)), Actinomycetospora chlora sp. nov. (type strain TT07I-57(T)  = NBRC 105900(T)  = KACC 14252(T)), Actinomycetospora cinnamomea sp. nov. (type strain IY07-53(T)  = NBRC 105527(T)  = KACC 14250(T)), Actinomycetospora corticicola sp. nov. (type strain 014-5(T)  = NBRC 103689(T)  = KACC 14253(T)), Actinomycetospora lutea sp. nov. (type strain TT00-04(T)  = NBRC 103690(T)  = KACC 14254(T)), Actinomycetospora straminea sp. nov. (type strain IY07-55(T)  = NBRC 105528(T)  = KACC 14251(T)) and Actinomycetospora succinea sp. nov. (type strain TT00-49(T)  = NBRC 103691(T)  = KACC 14255(T)).
Tamura
Tomohiko
T
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Ishida
Yuumi
Y
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Hamada
Moriyuki
M
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Otoguro
Misa
M
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Yamamura
Hideki
H
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
Hayakawa
Masayuki
M
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
Suzuki
Ken-Ichiro
KI
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000596:Amino Acids; D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S