Voluntary wheel running exercise and dietary lactose concomitantly reduce proportion of secondary bile acids in rat feces.
According to epidemiologic studies, a negative correlation exists between exercise amount and subsequent cancer development risk in the large intestine. The proportion of secondary bile acids (SBA) in the large intestine is related to subsequent risk for colorectal carcinogenesis. The aim of this study was to investigate the effects of voluntary wheel running exercise and dietary intervention on bile acid (BA) metabolism in the large intestine. Wistar/ST rats (6 wk old) were divided into two groups, exercise and sedentary, after acclimation. Four days after the animals were assigned to a group, rats in each group were fed diets supplemented with different carbohydrate sources including dextrin, sucrose, and lactose. The wheel running period was 4 wk in the exercise group, whereas rats in the sedentary group remained in individual cages during this period. BA composition in collected feces was analyzed with ultraperformance liquid chromatography-electrospray ionization mass spectrometry. We found that wheel running exercise decreased plasma concentrations of cholesterol, triglyceride, and free fatty acids. These decreases were accompanied by a reduction in the proportion of SBA to primary BA (PBA) in feces; however, daily excretion of BA was comparable regardless of wheel running exercise. In addition, ingestion of lactose decreased the SBA-to-PBA ratio and suppressed production of hyodeoxycholic acid in feces. In conclusion, voluntary wheel running exercise, in combination with dietary intervention, could independently reduce the SBA-to-PBA ratio within the large intestine without changing BA excretion. These changes may contribute to the prevention of colorectal carcinogenesis.
Hagio
Masahito
M
Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
Matsumoto
Megumi
M
Yajima
Takaji
T
Hara
Hiroshi
H
Ishizuka
Satoshi
S
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D002853:Chromatography, Liquid; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000517:prevention & control; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005243:Feces / Q000737:chemistry*; D007420:Intestine, Large / Q000378:metabolism*; D007785:Lactose / Q000008:administration & dosage*; D008297:Male; D005082:Physical Exertion; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D001647:Bile Acids and Salts; D004040:Dietary Carbohydrates; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D002784:Cholesterol; D007785:Lactose