title: Evaluation of specificity of BP3385 for Bordetella pertussis detection.
abstract: BP3385 has been proposed as a diagnostic PCR target for discriminating between Bordetella pertussis and other Bordetella species that also infect humans. Our results demonstrate that this gene is also present in some strains of Bordetella hinzii and Bordetella bronchiseptica.
authors.affiliation: Respiratory Diseases of Livestock Research Unit, USDA/ARS/National Animal Disease Center, P.O. Box 70, 1920 Dayton Road, Ames, IA 50010, USA. karen.register@ars.usda.gov
authors.forename: Karen B
authors.initials: KB
authors.lastname: Register
authors.forename: Tracy L
authors.initials: TL
authors.lastname: Nicholson
authors.forename: J L
authors.initials: JL
authors.lastname: Guthrie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000235:genetics; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014917:Whooping Cough / Q000175:diagnosis*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial