title: Regulation of L-type Ca++ currents and process morphology in white matter oligodendrocyte precursor cells by golli-myelin proteins.
abstract: The golli myelin basic proteins are expressed in oligodendroglial precursor cells (OPCs) where they play a role in regulating Ca(2+) homeostasis. During depolarization, they influence process outgrowth and migration through their action on voltage-operated Ca(2+) channels (VOCCs). To identify ion channels that are modulated by golli, we examined the electrophysiological properties of VOCCs in OPCs in the white matter of golli knock-out and control mice. OPCs exhibited two distinct Ca(2+) channels, which were distinguished by their voltage dependence and pharmacological profiles and which exhibited many of the hallmarks of LVA/T-type and HVA/L-type Ca(2+) channels. The density of high-voltage-activated (HVA) currents was reduced in OPCs recorded in golli-KO tissue, while low-voltage-activated (LVA) currents remained unaltered in these cells. These data indicate that golli exerts an exclusive influence on L-type Ca(2+) channels in OPCs. Oligodendrocytes (OLs) also displayed LVA and HVA currents, although the density of these currents was much reduced at this developmental stage. These currents were not altered in golli-KO OLs showing the influence of golli on L-type Ca(2+) channels is restricted to a specific time-window during the course of oligodendroglial development. The actions of golli on OPC L-type Ca(2+) channels were accompanied by changes in process morphology, including a reduction in process complexity and the appearance of enlarged varicosities that decorated these cellular processes. These data on L-type Ca(2+) channels and process development provide in situ evidence for the influence of golli on VOCCs, and offer an explanation for the hypomyelination observed in the brains of golli-KO mice.
authors.affiliation: Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, 90095, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fulton
authors.forename: Pablo M
authors.initials: PM
authors.lastname: Paez
authors.forename: Robin
authors.initials: R
authors.lastname: Fisher
authors.forename: Vance
authors.initials: V
authors.lastname: Handley
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Colwell
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Campagnoni
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000378:metabolism* / Q000502:physiology; D020013:Calcium Signaling / Q000235:genetics; D002413:Cations, Divalent / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis / Q000235:genetics; D015640:Ion Channel Gating / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D004676:Myelin Basic Protein; D009186:Myelin Sheath / Q000502:physiology; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D011499:Protein Processing, Post-Translational / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D020746:Calcium Channels, L-Type; D002413:Cations, Divalent; C499047:Mbp protein, mouse; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D014157:Transcription Factors