[Detection of serum proteins in the patients of lung adenocarcinoma by the method of magnetic bead based sample fractionation and MALDI-TOF-MS].
OBJECTIVE
To screen the serum proteins related to human lung adenocarcinoma using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technology.


METHODS
The blood samples were collected from 10 patients of lung adenocarcinoma before and one week after the surgery, while 10 healthy subjects were used as control. The differential protein expression between the two groups and the change of those proteins after surgery were studied by ClinProt magnetic bead enrichment and MALDI-TOF-MS.


RESULTS
Six protein peaks were identified, 2 of them were highly expressed protein biomarkers with relative molecular weights of 2661, 2991, and increased after the surgery, 4 of them were lowly expressed protein biomarkers with relative molecular weights of 4091, 4210, 4644, 5336, which continuously decreased after the surgery.


CONCLUSIONS
ClinProt magnetic bead enrichment and MALDI-TOF-MS is a quick, easy and sensitive method of proteomics. The differential expressed proteins may be the latent tumor marker of lung adenocarcinoma. The alteration of those proteins after surgery might be helpful to assess the therapeutic effect and prognosis.
Liu
Dan
D
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Lun-Xu
LX
Yuan
Quan
Q
Li
Xiao-Liang
XL
Huang
Na
N
Yang
Xiao-Dong
XD
D000230:Adenocarcinoma / Q000097:blood* / Q000601:surgery; D000368:Aged; D001798:Blood Proteins / Q000032:analysis*; D005591:Chemical Fractionation / Q000379:methods; D019411:Clinical Laboratory Techniques / Q000379:methods; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000601:surgery; D008280:Magnetics; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001798:Blood Proteins; D020543:Proteome