Characterization of a fenpropathrin-degrading strain and construction of a genetically engineered microorganism for simultaneous degradation of methyl parathion and fenpropathrin.
A gram-negative fenpropathrin-degrading bacterial strain Sphingobium sp. JQL4-5 was isolated from the wastewater treatment sludge of an insecticide factory. Strain JQL4-5 showed the ability to degrade other pyrethroid insecticides, but it was not able to degrade methyl parathion. To enhance its degrading range of substrate, a methyl parathion hydrolase gene (mpd) was successfully introduced into the chromosome of strain JQL4-5 with a mini-Tn-transposon system. A genetically engineered microorganism (GEM) named JQL4-5-mpd resulted, which was capable of simultaneously degrading methyl parathion and fenpropathrin. Soil treatment results indicated that JQL4-5-mpd is a promising multifunctional bacterium in the bioremediation of multiple pesticide-contaminated environments.
Hong
Yuanfan
Y
Key Lab of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
Zhou
Jin
J
Hong
Qing
Q
Wang
Qian
Q
Jiang
Jiandong
J
Li
Shunpeng
S
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002875:Chromosomes; D005818:Genetic Engineering / Q000379:methods; D006867:Hydrolases / Q000235:genetics*; D007220:Industrial Waste; D007306:Insecticides / Q000378:metabolism*; D008743:Methyl Parathion / Q000378:metabolism*; D011722:Pyrethrins / Q000378:metabolism*; D012722:Sewage; D012989:Soil Pollutants / Q000378:metabolism*
D007220:Industrial Waste; D007306:Insecticides; D011722:Pyrethrins; D012722:Sewage; D012989:Soil Pollutants; D008743:Methyl Parathion; C044267:fenpropathrin; D006867:Hydrolases