title: [Mouthwash solutions containing microencapsuled natural extracts: In vitro evaluation of antioxidant properties (dental plaque and gingivitis)].
abstract: BACKGROUND
  New mouthwash solutions containing microencapsulated natural extracts were developed to provide both antiseptic activity and in depth treatment of oral tissues, due to their antioxidant and immunoregulatory properties. The objective of this study was to quantify the antioxidant action of the GingiNat solution (LoB5 Foundation, Paris, France) in an in vitro cell model.
  
  
  METHODS
  Diluted GingNat solutions (0.12%, 0.06% and 0.012%) were put in contact with Jurkat type human lymphoid cells in basal radical state (cells at rest) and in provoked oxidative stress conditions (after an UVA+UVB irradiation). The lipid peroxidation was quantified by flow cytometry using a fluorescent probe.
  
  
  RESULTS
  The diluted GingNat solutions at 0.12%, 0.06%, and 0.012% showed a significant antioxidant effect with respectively 122.9%, 117.8% and 119.3% on average. The difference was statistically significant compared to controls for the three concentrations without any significant difference among them. This antioxidant effect was even more significant when cells were in oxidative stress with respectively 155.3%, 139.3%, and 132.5% on average. There was a significant difference between the tested concentrations (p<0.01).
  
  
  CONCLUSIONS
  These first in vitro results confirmed the antioxidant properties of the GingiNat solution. These antioxidant properties are significantly higher at stronger concentrations. Further studies are required to analyze the influence of microencapsulation on these results. Clinical trials are needed to confirm these antioxidant properties.
authors.affiliation: Department of Biomaterials, Institute for Clinical Sciences, the Sahlgrenska Academy at University of Gothenburg, Medicinaregatan 8B, 41390 Gothenburg, Suède.
authors.forename: J
authors.initials: J
authors.lastname: Mouhyi
authors.forename: M
authors.initials: M
authors.lastname: Del Corso
authors.forename: M-P
authors.initials: MP
authors.lastname: Hippolyte
authors.forename: G
authors.initials: G
authors.lastname: Sammartino
authors.forename: D M
authors.initials: DM
authors.lastname: Dohan Ehrenfest
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D018929:Cell Culture Techniques; D004339:Drug Compounding; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry; D019793:Fluorescein; D005456:Fluorescent Dyes; D006801:Humans; D019169:Jurkat Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D008422:Materials Testing; D009067:Mouthwashes / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010636:Phenols / Q000494:pharmacology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D059808:Polyphenols; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D005456:Fluorescent Dyes; D009067:Mouthwashes; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; C024070:pycnogenols; D019793:Fluorescein