title: A metabolomic approach to study major metabolite changes during acclimation to limiting CO2 in Chlamydomonas reinhardtii.
abstract: Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high- and low-CO(2)-grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO(2). The biggest differences were typically observed 3 h after transfer to low-CO(2) conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO(2) treatment to high-CO(2) control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.
authors.affiliation: Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Umea, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Renberg
authors.forename: Annika I
authors.initials: AI
authors.lastname: Johansson
authors.forename: Tatiana
authors.initials: T
authors.lastname: Shutova
authors.forename: Hans
authors.initials: H
authors.lastname: Stenlund
authors.forename: Anna
authors.initials: A
authors.lastname: Aksmann
authors.forename: John A
authors.initials: JA
authors.lastname: Raven
authors.forename: Per
authors.initials: P
authors.lastname: Gardeström
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
authors.forename: Göran
authors.initials: G
authors.lastname: Samuelsson
mesh_terms: D000064:Acclimatization / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D013997:Time Factors
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen