Multilineage differentiation of muscle-derived stem cells from GFP transgenic mice.
It is unclear whether green fluorescent protein (GFP) expression is maintained during the course of multilineage differentiation of muscle-derived stem cells (MDSCs). We isolated MDSCs from GFP-transgenic mice and transferred them to chondrogenic, neurogenic or myogenic media. Multilineage differentiation was examined by morphological observation, histological staining, immunocytochemical staining, real-time RT-PCR and Western blot. Both differentiated cells and non-differentiated cells maintained stable GFP expression until the cells exhibited a senescent phenotype. Thus, MDSCs from GFP-transgenic mice have multilineage potential in vitro and that GFP expression does not influence the multilineage potential of MDSCs (or vice versa).
Ye
Chuan
C
Department of Orthopaedics, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China. yechuanchina@hotmail.com
Li
Jiangwei
J
He
Zixu
Z
Nin
Xu
X
Zhang
Yi
Y
Shang
Xianwen
X
Liu
Riguang
R
Duan
Yiqiang
Y
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003470:Culture Media / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000166:cytology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*
D003470:Culture Media; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins