title: Development of fluorescent array based on sol-gel/chitosan encapsulated acetylcholinesterase and pH sensitive oxazol-5-one derivative.
abstract: A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane/chitosan matrix via a microarrayer. The constructed tetraethoxysilane/chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 × 10(-8) M, with a detection limit of 0.27 × 10(-8) M.
authors.affiliation: Faculty of Arts and Sciences, Department of Chemistry, University of Dokuz Eylul, Izmir, Turkey. gulsiye.ozturk@deu.edu.tr
authors.forename: Gulsiye
authors.initials: G
authors.lastname: Ozturk
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Feller
authors.forename: Kay
authors.initials: K
authors.lastname: Dornbusch
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.forename: Serap
authors.initials: S
authors.lastname: Alp
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Ergun
mesh_terms: D000109:Acetylcholine / Q000032:analysis; D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010080:Oxazoles / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D010080:Oxazoles; D048271:Chitosan; D000110:Acetylcholinesterase; D000109:Acetylcholine