title: Proteomic analysis of CD4+ T-lymphocytes in patients with asthma between typical therapy (controlled) and no typical therapy (uncontrolled) level.
abstract: T-lymphocyte (T-LC)-derived cytokines have been implicated in asthmatic pathogenesis. Proteomic technology is now widely accepted as a complementary technology to genetic profiling. We investigated the changes of proteins in T-LC of asthmatic patients from the no typical therapy (uncontrolled) to typical therapy (controlled) level by using standard proteome technology.
  
  
  METHODS
  The proteins of CD4+ T-LC were isolated from the whole blood of six asthmatic patients from uncontrolled to controlled levels over 3 months. Two-dimensional polyacrylamide gel electrophoresis was performed and coomassie blue stained protein spots were comparatively analyzed by using an image analyzer. Some differentially expressed spots were identified by liquid chromatography/mass spectrometry and database search. Our results showed that 13 proteins showed different expression. Six protein spots in the CD4+ T-LC of the uncontrolled asthmatic patients were increased and 7 spots were decreased compared to those of the controlled subjects. In conclusion, the proteomic examination of the CD4+ T-LC revealed some differentially expressed proteins in the uncontrolled and controlled asthmatic patients. The possibility of using the differentially expressed proteins as important biomarkers and therapeutic targets warrants further study.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, St Martin De Porres Hospital, Chiayi, Taiwan.
authors.forename: Yang-Ching
authors.initials: YC
authors.lastname: Ko
authors.forename: Wu-Huei
authors.initials: WH
authors.lastname: Hsu
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
authors.forename: Mong-Ping
authors.initials: MP
authors.lastname: Dai
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Ou
authors.forename: Wen-Pin
authors.initials: WP
authors.lastname: Wu
mesh_terms: D000328:Adult; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use; D001249:Asthma / Q000097:blood* / Q000188:drug therapy / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008168:Lung; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D018927:Anti-Asthmatic Agents; D015415:Biomarkers; D016207:Cytokines