title: Functional evidence for a twisted conformation of the NMDA receptor GluN2A subunit N-terminal domain.
abstract: Ionotropic glutamate receptors (iGluRs) possess in their extracellular region a large N-terminal domain (NTD) that precedes the agonist-binding domain and displays a clamshell-like architecture similar to the bacterial leucine/isoleucine/valine-binding protein (LIVBP). In addition to their role in receptor assembly, in NMDA receptors (NMDARs), the NTDs of GluN2A and GluN2B subunits form a major site for subunit-specific regulation of ion channel activity, in particular through binding of allosteric modulators such as the synaptically-enriched zinc ion. A recent crystallographic study of the isolated GluN2B NTD has revealed an unexpected twisted closed-cleft conformation caused by a rotation of ∼ 50° in the interlobe orientation compared with all other known LIVBP-like structures (Karakas et al., 2009). By measuring currents carried by recombinant NMDARs, we now provide functional evidence, through disulfide cross-linking and the identification of a new zinc-binding residue (D283), that the GluN2A NTD of intact GluN1/GluN2A receptors adopts a similar twisted conformation in its closed-cleft state. We propose that the twisted NTD conformation is a distinct structural feature of NMDARs (at least for GluN2A and GluN2B subunits), arguing for interactions between the NTDs in the tetrameric complex that are likely to differ between NMDA and AMPA/kainate receptors.
authors.affiliation: Institut de Biologie, CNRS UMR8197, INSERM U1024, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris, France.
authors.forename: David
authors.initials: D
authors.lastname: Stroebel
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Carvalho
authors.forename: Pierre
authors.initials: P
authors.lastname: Paoletti
mesh_terms: D000494:Allosteric Regulation / Q000502:physiology*; D001665:Binding Sites; D011487:Protein Conformation; D021122:Protein Subunits / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate; D015032:Zinc