title: Characterization of two Paenibacillus amylolyticus strain 27C64 pectate lyases with activity on highly methylated pectin.
abstract: Two pectate lyases were identified from Paenibacillus amylolyticus 27C64; both enzymes demonstrated activity on methylated pectin in addition to polygalacturonic acid. PelA is in a subclass of the pectate lyase family III. PelB shows some features of pectate lyase family I but is highly divergent.
authors.affiliation: Department of Microbiology, 1000 Cedar Street, 546 Biological Sciences, University of Georgia, Athens, GA, USA.
authors.forename: Whitney E
authors.initials: WE
authors.lastname: Boland
authors.forename: Emily DeCrescenzo
authors.initials: ED
authors.lastname: Henriksen
authors.forename: Joy
authors.initials: J
authors.lastname: Doran-Peterson
mesh_terms: D002122:Calcium Chloride / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics; D010368:Pectins / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D003067:Coenzymes; D004269:DNA, Bacterial; D010368:Pectins; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D002122:Calcium Chloride; C003181:polygalacturonic acid