title: Evaluation of the specific interactions between glycodendrimeric porphyrins, free or incorporated into liposomes, and concanavalin A by fluorescence spectroscopy, surface pressure, and QCM-D measurements.
abstract: In photodynamic therapy, the specificity of a photosensitizer and its penetration into tumor cells are crucial. We have analyzed the ability of newly synthesized meso-(tetraphenyl)porphyrins to be recognized by a model of mannose-specific proteins overexpressed at the surface of retinoblastoma cells. The specific interaction of porphyrin with Con A was studied by surface pressure measurements, fluorescence spectroscopy, dynamic light scattering, and QCM-D. The extent of porphyrins binding to Con A was highly dependent upon their chemical structure. Glycodendrimeric porphyrins showed the higher binding constant to Con A. The length of the spacer separating the sugar from the tetrapyrrolic ring appeared to be crucial in controlling the interaction of the compounds with the lectin in solution or immobilized onto a solid substrate. The methodology used proved to be efficient for the selection of potentially active compounds. The glycodendrimeric porphyrins, especially the derivative having the longer spacer, interacted more significantly with the lectin than the compound devoid of any sugar.
authors.affiliation: Univ Paris-Sud 11, UMR 8612, Laboratoire de Physico-Chimie des Surfaces, IFR 141, F-92296 Châtenay-Malabry, France.
authors.forename: A
authors.initials: A
authors.lastname: Makky
authors.forename: J P
authors.initials: JP
authors.lastname: Michel
authors.forename: A
authors.initials: A
authors.lastname: Kasselouri
authors.forename: E
authors.initials: E
authors.lastname: Briand
authors.forename: Ph
authors.initials: P
authors.lastname: Maillard
authors.forename: V
authors.initials: V
authors.lastname: Rosilio
mesh_terms: D003208:Concanavalin A / Q000737:chemistry; D050091:Dendrimers / Q000138:chemical synthesis*; D008081:Liposomes / Q000737:chemistry*; D008956:Models, Chemical; D017319:Photosensitizing Agents / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013499:Surface Properties
chemical_list: D050091:Dendrimers; D008081:Liposomes; D017319:Photosensitizing Agents; D011166:Porphyrins; D003208:Concanavalin A