Non-ribosomal peptides produced by Brazilian cyanobacterial isolates with antimicrobial activity.
Cyanobacterial strains isolated from terrestrial and freshwater habitats in Brazil were evaluated for their antimicrobial and siderophore activities. Metabolites of fifty isolates were extracted from the supernatant culture media and cells using ethyl acetate and methanol, respectively. The extracts of 24 isolates showed antimicrobial activity against several pathogenic bacteria and one yeast. These active extracts were characterized by Q-TOF/MS. The cyanobacterial strains Cylindrospermopsis raciborskii 339-T3, Synechococcus elongatus PCC7942, Microcystis aeruginosa NPCD-1, M. panniformis SCP702 and Fischerella sp. CENA19 provided the most active extracts. The 50 cyanobacterial strains were also screened for the presence of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes and microcystin production. Putative fragment genes coding for NRPS adenylation domains and PKS keto-synthase domains were successfully PCR amplified from 92% and 80% of cyanobacterial strains, respectively. The potential therapeutical compounds siderophores were detected in five cyanobacterial isolates. Microcystin production was detected by ELISA test in 26% of the isolates. Further a protease inhibitor substance was detected by LC-MS/MS in the M. aeruginosa NPLJ-4 extract and the presence of aeruginosin and cyanopeptolin was confirmed by PCR amplification using specific primers, and sequenced. This screening study showed that Brazilian cyanobacterial isolates are a rich source of natural products with potential for pharmacological and biotechnological applications.
Silva-Stenico
Maria Estela
ME
University of São Paulo, Center for Nuclear Energy in Agriculture, Molecular Ecology of Cyanobacteria Laboratory, 13400-970, Piracicaba-SP, Brazil.
Silva
Caroline Souza Pamplona
CS
Lorenzi
Adriana Sturion
AS
Shishido
Tânia Keiko
TK
Etchegaray
Augusto
A
Lira
Simone Possedente
SP
Moraes
Luiz Alberto Beraldo
LA
Fiore
Marli Fátima
MF
D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D001938:Brazil; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D055808:Drug Discovery; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D052998:Microcystins / Q000096:biosynthesis; D010453:Peptide Synthases / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000032:analysis / Q000302:isolation & purification; D017262:Siderophores / Q000302:isolation & purification / Q000378:metabolism*
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D052998:Microcystins; D011480:Protease Inhibitors; D017262:Siderophores; C078588:microcystin; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase