GADD34 suppresses wound healing by upregulating expression of myosin IIA.
Wound healing consists of sequential steps of tissue repair, and cell migration is particularly important. In order to analyze the potential function of growth arrest and DNA damage inducible protein 34 (GADD34) in tissue repair, we performed in vitro and in vivo wound healing experiments. In an in vitro scratch assay, GADD34 knockout (KO) mouse embryonic fibroblasts (MEFs) had higher migration rates than did wild type (WT) MEFs. Furthermore, the rate of wound closure was faster in GADD34 KO MEFs than in WT MEFs. Using in vivo punch biopsy assays, GADD34 KO mice had accelerated wound healing compared to WT mice. WT mice expressed higher amounts of myosin IIA in migrating macrophages and myofibroblasts than did GADD34 KO mice. These results indicate that GADD34 negatively regulates cell migration in wound healing via expression of myosin IIA.
Tanaka
Chie
C
Department of Immunology, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa-ku, Nagoya, Aichi, 466-8520, Japan.
Ito
Sachiko
S
Nishio
Naomi
N
Kodera
Yasuhiro
Y
Sakurai
Hidetoshi
H
Suzuki
Haruhiko
H
Nakao
Akimasa
A
Isobe
Ken-Ichi
K
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D024507:Nonmuscle Myosin Type IIA / Q000235:genetics* / Q000378:metabolism; D054645:Protein Phosphatase 1; D015854:Up-Regulation / Q000235:genetics; D014945:Wound Healing / Q000235:genetics*
D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; C513256:Ppp1r15a protein, mouse; D054645:Protein Phosphatase 1; D024507:Nonmuscle Myosin Type IIA