Therapeutic removal of amyloid deposits in cutaneous amyloidosis by localised intra-lesional injections of anti-amyloid antibodies.
In the skin, amyloidosis can be found with or without systemic disease. Primary cutaneous amyloidosis defines those amyloidoses restricted to the skin without involvement of other systems. Here, we used conformation-specific antibodies to characterise both fibrillar and oligomeric amyloid aggregates in the skin from patients with cutaneous amyloidosis. Localised cutaneous amyloidosis with different morphology was reproduced in mice by intra-dermal (i.d.) and subdermal administration of amyloid-enhancing factor. Moreover, we demonstrated that conformational antibodies were effective in clearing amyloid deposits caused by localised intra-lesional injections without the necessity of an immune response. Given the accessibility and amyloid localization in this disease, direct i.d. injections of conformational antibodies could be a convenient and direct method for treatment.
Clos
Audra L
AL
Department of Neurology, University of Texas Medical Branch, George and Cynthia Mitchell Center for Neurodegenerative Diseases, Galveston, TX 77555, USA.
Lasagna-Reeves
Cristian A
CA
Wagner
Richard
R
Kelly
Brent
B
Jackson
George R
GR
Kayed
Rakez
R
D000682:Amyloid / Q000276:immunology*; D000686:Amyloidosis / Q000276:immunology / Q000473:pathology / Q000628:therapy; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods*; D007271:Injections, Intradermal; D015552:Injections, Intralesional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012871:Skin Diseases / Q000276:immunology / Q000473:pathology / Q000628:therapy
D000682:Amyloid; D000906:Antibodies