title: Relevance of miR-21 and miR-143 expression in tissue samples of colorectal carcinoma and its liver metastases.
abstract: MicroRNAs, which are endogenously expressed regulatory noncoding RNAs, have an altered expression in colorectal cancer. The aim of our study was to assess the relationship of miR-21 and miR-143 expression to the prognostic/clinicopathological features of colorectal carcinoma (CRC) and colorectal liver metastases (CLM). The estimation was performed in 46 paired (tumor and control) tissue samples of CRC. Further, we studied 30 tissue samples of CLM. MiR-21 and miR-143 expressions were quantified by using the quantitative reverse transcription polymerase chain reaction method. Relation of miR-21 and miR-143 expression to disease-free interval (DFI) (Wilcoxon; P = 0.0026 and P = 0.0191, respectively) was recorded. There was shorter DFI in patients with a higher expression of miR-21 and, surprisingly, also in patients with a higher expression of miR-143, which is a putative tumor suppressor. There was a higher expression of miR-21 and lower expression of miR-143 in CRC tissue in comparison with adjacent normal colon tissue (P < 0.0001; P < 0.0001, respectively). Similarly, we observed a higher expression of miR-21 and a lower expression of miR-143 in CLM in comparison with normal colon tissue (P < 0.0001; P < 0.0001, respectively). Our results support the hypothesis about oncogenic function of miR-21 and show its relation to DFI. The role of miR-143 in carcinogenesis seems to be more complex.
authors.affiliation: Department of Biochemistry, Faculty of Medicine in Pilsen, Charles University in Prague, Czech Republic.
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Kulda
authors.forename: Martin
authors.initials: M
authors.lastname: Pesta
authors.forename: Ondrej
authors.initials: O
authors.lastname: Topolcan
authors.forename: Vaclav
authors.initials: V
authors.lastname: Liska
authors.forename: Vladislav
authors.initials: V
authors.lastname: Treska
authors.forename: Alan
authors.initials: A
authors.lastname: Sutnar
authors.forename: Karel
authors.initials: K
authors.lastname: Rupert
authors.forename: Marie
authors.initials: M
authors.lastname: Ludvikova
authors.forename: Vaclav
authors.initials: V
authors.lastname: Babuska
authors.forename: Lubos
authors.initials: L
authors.lastname: Holubec
authors.forename: Radim
authors.initials: R
authors.lastname: Cerny
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D008875:Middle Aged
chemical_list: C542359:MIRN143 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs