Porcine islet isolation outcome is not affected by the amount and distribution of collagen in the pancreas.
Variable islet yields in porcine islet isolation may be caused by the collagen substrate within the pancreas. The aim of the present study was to determine the total amount and distribution of collagen within porcine pancreata and their relationship to islet isolation outcome. A total of 64 juvenile and 76 adult porcine pancreata of eight purebred breeds were histologically examined. The amount of collagen was quantitatively assessed in tissue samples stained with Sirius Red. Collagen distribution was semi-quantitatively determined by assessing the presence of collagen in the endocrine-exocrine interface and within the islet, in tissue samples stained with Sirius Red and anti-insulin. Islet isolation was performed in 58 pancreata of the adult group. Total collagen content and islet encapsulation ranged widely in both adult and juvenile pigs. However, the majority of islets in adult and juvenile pigs had no or only a limited collagen capsule. The difference in collagen content between adult and juvenile pigs could not be explained by age. Furthermore, no differences between adult and juvenile pigs were found in islet encapsulation or the amount of intra-islet collagen. In adult pigs, no significant relationships were found between obtained islet yield and total collagen content, islet encapsulation or amount of collagen within the islet. Considering the limitations in experimental design (staining method) and study material, isolation outcome does not seem to be affected by the total collagen content or collagen distribution. The influence of other matrix elements and collagen subtypes should be investigated.
Hilling
Denise E
DE
Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands. d.e.hilling@lumc.nl
Rijkelijkhuizen
Josephine K R A
JK
Töns
H Annemiek M
HA
Terpstra
Onno T
OT
Bouwman
Eelco
E
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003094:Collagen / Q000032:analysis*; D004339:Drug Compounding; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation; D010179:Pancreas / Q000737:chemistry* / Q000166:cytology*; D013552:Swine
D003094:Collagen