Chemical cytometry: fluorescence-based single-cell analysis.
Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ antibody-based stains to characterize a handful of components in single cells. Chemical cytometry, in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.
Cohen
Daniella
D
Department of Chemistry, University of Washington, Seattle, 98195, USA.
Dickerson
Jane A
JA
Whitmore
Colin D
CD
Turner
Emily H
EH
Palcic
Monica M
MM
Hindsgaul
Ole
O
Dovichi
Norman J
NJ
D000818:Animals; D001679:Biogenic Amines / Q000737:chemistry; D003592:Cytophotometry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009696:Nucleic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
D001679:Biogenic Amines; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D011506:Proteins