title: Repetitive low-volume blood sampling method as a feasible monitoring tool in a mouse model of sepsis.
abstract: Blood-based monitoring of immunoinflammatory and organ function fluctuations is essential in models of critical illness. This is challenging in diseased mice as repetitive blood collection may be harmful and/or affect end points. We studied the influence of daily sampling in acutely septic (days 1-5) mice upon survival and selected hematologic and organ function parameters. In addition, we tested the reliabilty of complete blood cell (CBC) count using resuspended blood cells. Female OF-1 and CD-1 mice underwent cecal ligation and puncture (CLP) and were subdivided into Daily and Day 5 groups. Blood was collected daily for 5 days in the Daily group and only on day 5 post-CLP in the Day 5 group. We tested 20 μL (both strains) and 35 μL (OF-1 mice) sampling volumes. The 35-μL volume simultaneously served to test the CBC reliabilty in resuspended versus unprocessed blood. Daily sampling did not affect the 14-day CLP mortality. Compared with the Day 5 group, daily 35-μL sampling in OF-1 mice decreased the red blood cell count and hemoglobin concentration by 22% and 23% (P < 0.05). In neither strain did daily 20-μL sampling affect the red blood cell count, whereas there was a 9% hemoglobin decrease (P < 0.05) in OF-1 mice. Although alanine aminotransferase, lactate dehydrogenase, and glucose levels were comparable, urea significantly increased by 24% in the Daily group (20-μL volume, OF-1 mice). Interleukin 6, platelets, and white blood cell counts remained unaffected. There was an excellent correlation between regular and resuspended CBC for all cell types (r ≥ 0.9; slope, ≥0.9), except lymphocytes (r > 0.5; slope, >0.5). This method provides a feasible and safe translation of clinically relevant daily immunomonitoring to the mouse sepsis model.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the Trauma Research Center of AUVA, Vienna, Austria.
authors.forename: Katrin M
authors.initials: KM
authors.lastname: Weixelbaumer
authors.forename: Pierre
authors.initials: P
authors.lastname: Raeven
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Martijn
authors.initials: M
authors.lastname: van Griensven
authors.forename: Soheyl
authors.initials: S
authors.lastname: Bahrami
authors.forename: Marcin F
authors.initials: MF
authors.lastname: Osuchowski
mesh_terms: D000818:Animals; D002432:Cecum / Q000293:injuries*; D004195:Disease Models, Animal; D005260:Female; D015850:Interleukin-6 / Q000097:blood; D007416:Intestinal Perforation / Q000097:blood; D051379:Mice; D010538:Peritonitis / Q000097:blood / Q000209:etiology; D011677:Punctures / Q000009:adverse effects; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology
chemical_list: D015850:Interleukin-6