[Comparison of different ELISAs for detection of antibodies to swine vesicular disease virus in sera from experimentally infected animals].
The study has shown the efficiency of a competitive ELISA (C-ELISA) variant or an indirect ELISA (I-ELUSA) in the detection of antibodies to swine vesicular disease virus (SVDV) versus traditional assays, such as a microneutralization test, a blocking ELIDA test, and a the reference test Ceditest SVDV (Cedi-Diagnostics B.V., Netherlands). Specific antibodies in the pig sera could be detected by C-ELISA on days 4-5 and by I-ELISA on day 6 after experimental SVDV infection. Specific antibodies were detected in a contact pig 11 days after the beginning of the experiment.
Lugovskaia
N N
NN
Kharitonova
E N
EN
Nikiforov
V V
VV
Zhil'tsova
M V
MV
Krementsugskaia
S R
SR
Borisov
V V
VV
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D029822:Enterovirus B, Human / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D019084:Fluorescent Antibody Technique, Indirect; D013552:Swine; D013555:Swine Vesicular Disease / Q000097:blood / Q000175:diagnosis*
D000914:Antibodies, Viral