Analgesic effects of the ethanolic extract from Magnolia ovata (Magnoliaceae) trunk bark and of N-acetylxylopine, a semi-synthetic analogue of xylopine.
This study investigated the antinociceptive effects of the ethanolic extract (EEMO) obtained from Magnolia ovata (A.St.-Hil.) Spreng and N-acetylxylopine (AXyl), a stable derivative of xylopine in different models of nociception. The EEMO and AXyl inhibited the nociception induced by acetic acid in mice, in a dose-dependent manner with a maximal inhibition of 91 ± 9% and 50 ± 11%, respectively. Oral administration of EEMO or AXyl also significantly inhibited the inflammatory phase of formalin-induced nociception with maximal reduction of 87 ± 3.9% and 71 ± 10%, respectively. Confirming the effectiveness of the extract and the isolated compound in inflammatory responses, EEMO or AXyl inhibited carrageenan-induced mechanical allodynia with percentage of inhibition of 40 ± 6% for EEMO and 82 ± 8% for AXyl. Intraplantar injection of AXyl in the ipsilateral paw, but not in the contralateral paw, also reduced carrageenan-induced mechanical allodynia in mice. The response of the animals for maximal doses tested of EEMO and AXyl in the hot-plate or rota-rod models were not altered. These results show that the extract from M. ovata and the stable derivative AXyl possess analgesic properties towards inflammatory pain acting on peripheral sites.
Mori
Lídia Sayuri
LS
Department of Pharmacology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Boller
Shirley
S
Kassuya
Cândida Aparecida Leite
CA
Stefanello
Maria Élida Alves
MÉ
Zampronio
Aleksander Roberto
AR
D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001060:Aporphines / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy*; D029562:Magnoliaceae / Q000737:chemistry*; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018547:Plant Stems
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D001060:Aporphines; D010936:Plant Extracts; D005557:Formaldehyde; C062700:xylopine; D002351:Carrageenan; D019342:Acetic Acid