[Isolation of mesenchymal stem cells from human umbilical cord blood and its bone inducing activity in vitro].
OBJECTIVE
To investigate the biological characters of mesenchymal stem cells obtained from human umbilical cord blood(HUCB-MSCs), and detect the influence of different serum concentrations of culture medium to primary HUCB-MSCs' growth kinetics and the osteogenic differentiation capacity of the passage cells.


METHODS
The mononuclear cells were isolated from umbilical cord blood(UCB), the growth characters and morphology of the primary MSCs were observed. Primary cells were cultured in alpha-MEM with different serum concentrations, and immunophenotype was investigated. P3 cells were induced to osteoblast-like cells and the differentiation results were analysed. SPSS 16.0 software package was used for statistical analysis through repeated measures analysis of variances.


RESULTS
Four subpopulations with different morphology could be distinguished in primary HUCB-MSCs. All the subpopulations were positive for CD44 and CD105, and negative for CD34 and CD45. HUCB-MSCs displayed faster and stable growth when cultured in alpha-MEM containing 15% serum. Passage cells of HUCB-MSCs could be induced into osteoblast-like cells, the formation of mineralized extracellular matrix and the positive staining of expression of ALP could be observed.


CONCLUSIONS
HUCB-MSCs are high in viability but low in proliferation. HUCB-MSCs contain 4 subpopulations with different morphology. The appropriate concentration of serum in culture medium should be 15%. HUCB-MSCs could be induced into osteoblast-like cells in vitro.
Zhang
He
H
Department of Orthodontics, Stomatological Hospital of Xi'an Jiaotong University, Xi'an 710004, China. bluewater1123@yeah.net
Li
Ang
A
Rao
Guo-zhou
GZ
Zhou
Hong
H
D002454:Cell Differentiation; D005312:Fetal Blood; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D009930:Organic Chemicals; D010006:Osteoblasts; D010012:Osteogenesis
D009930:Organic Chemicals; C420642:alpha minimal essential medium