Identification of putative biomarkers for toluene-degrading Burkholderia and pseudomonas by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting.
The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.
Hartmann
Erica M
EM
Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Colquhoun
David R
DR
Halden
Rolf U
RU
D001673:Biodegradation, Environmental; D015415:Biomarkers / Q000032:analysis; D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010449:Peptide Mapping; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014050:Toluene / Q000378:metabolism*
D015415:Biomarkers; D014050:Toluene