title: Microtopographical regulation of adult bone marrow progenitor cells chondrogenic and osteogenic gene and protein expressions.
abstract: Microtopographic features affect diverse cell behaviors. Adult bone marrow progenitor cells (AMPCs) constitute a multipotent heterogeneous population. We hypothesized that microtopographies could direct AMPCs lineage-specific differentiation. AMPCs isolated from Sprague-Dawley rats were CD45 depleted, expanded, and plated at 10(5) cells/cm2 on epoxy-microfabricated: (1) 60-microm-deep grooves with 95-microm pitch (D60P95), (2) 55-microm-wide and 10-microm-deep squares (W55D10), (3) 30-microm-deep grooves with 45-microm pitch (D30P45), (4) 17-microm-wide and 10-microm-deep pillars (W17D10), and (5) smooth control. AMPCs were cultured using expansion, chondrogenesis, or osteogenesis supporting media. Cell cultures were examined by scanning electron microscopy, qRT-PCR, and immunostaining at 2, 9, 16, and 23 days after plating. Expressions of osteogenesis-related genes, such as Runx-2, alkaline phosphatase, osteopontin, osteocalcin, and parathyroid hormone-related protein receptor (PTHr), and chondrogenesis-associated genes, such as Sox-9, type II collagen, and aggrecan, were determined. In expansion medium, W55D10 induced a transient increase of Sox9 expression. Compared with smooth surfaces, type II collagen mRNA and protein expressions in chondrogenic medium were significantly upregulated on W55D10 by day 23. In contrast, osteocalcin and PTHr expressions were significantly increased on D30P45 in osteogenic medium. We have demonstrated that W55D10 and D30P45 enhanced AMPCs chondrogenic and osteogenic terminal differentiation with appropriate culture conditions.
authors.affiliation: Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, 2199 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 1Z3.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Kusano
authors.forename: H
authors.initials: H
authors.lastname: Frei
authors.forename: F
authors.initials: F
authors.lastname: Rossi
authors.forename: D M
authors.initials: DM
authors.lastname: Brunette
authors.forename: E E
authors.initials: EE
authors.lastname: Putnins
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000235:genetics*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D024043:Collagen Type II; D015675:Osteocalcin