Interleukin-1β contributes to dopaminergic neuronal death induced by lipopolysaccharide-stimulated rat glia in vitro.
Inflammation is involved in the pathology of Parkinson's disease, a disorder characterised by degeneration of dopaminergic neurons. This study demonstrates that conditioned medium (CM) from lipopolysaccharide (LPS)-treated rat glial-enriched cortical cultures induced death of embryonic rat dopaminergic neurons in vitro, an effect which was additive to the toxicity of the neurotoxin 6-hydroxydopamine. Interleukin-1β (IL-1β) in the CM may mediate this neuronal death. IL-1R1 was found to be expressed on dopaminergic neurons. Blockade of IL-1R1 prevented CM-induced dopaminergic neuronal death. This study suggests that IL-1β in CM from LPS-stimulated glia contributes to dopaminergic neuronal death induced by glia-conditioned medium.
Long-Smith
Caitríona M
CM
Department of Anatomy and Neuroscience, University College Cork, Ireland.
Collins
Louise
L
Toulouse
André
A
Sullivan
Aideen M
AM
Nolan
Yvonne M
YM
D018663:Adrenergic Agents / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D018501:Antirheumatic Agents / Q000494:pharmacology; D001921:Brain / Q000166:cytology*; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009457:Neuroglia / Q000737:chemistry / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D018663:Adrenergic Agents; D018501:Antirheumatic Agents; D017077:Culture Media, Conditioned; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine