Inorganic-binding peptides as tools for surface quality control.
This paper highlights an innovative application of inorganic-binding peptides as quality control tools for detecting defects on inorganic surfaces of any shape. The approach involves attaching a fluorescent label to an inorganic-binding peptide and exploiting the peptide's high binding specificity to detect, by simple fluorescence microscopy, chemical composition defects of microm size and crystallographic state defects. Proof of concept was demonstrated by monitoring binding of a previously isolated ZnO-binding peptide to galvanized steel substrates. The approach was further validated for TiO(2) coatings and stainless steel, with two new, specific inorganic-binding peptides isolated by phage display.
Vreuls
Christelle
C
Laboratoire de Biologie Moléculaire et de Génie Génétique, GIGA-R, B34 Sart-Tilman, University of Liège, Liège, Belgium. c.vreuls@ulg.ac.be
Zocchi
Germaine
G
Genin
Alexis
A
Archambeau
Catherine
C
Martial
Joseph
J
Van de Weerdt
Cécile
C
D000595:Amino Acid Sequence; D007287:Inorganic Chemicals / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011786:Quality Control; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000378:metabolism; D015034:Zinc Oxide / Q000737:chemistry / Q000378:metabolism
D007287:Inorganic Chemicals; D019151:Peptide Library; D010455:Peptides; D013193:Stainless Steel; C009495:titanium dioxide; D014025:Titanium; D015034:Zinc Oxide