title: Erosion-inhibiting potential of a stannous chloride-containing fluoride solution under acid flow conditions in vitro.
abstract: OBJECTIVE
  This study aimed to analyse the erosion-inhibiting potential of a single application of stannous chloride-containing fluoride solution on pellicle-covered enamel and dentine under constant acid flow conditions in vitro.
  
  
  METHODS
  Bovine enamel (n=60) and dentine (n=60) samples were exposed 1h to the oral cavity of 4 healthy volunteers to allow for in situ pellicle formation. Pellicle-covered samples were randomly assigned to three groups (each n=20 enamel and n=20 dentine samples; 5 enamel and 5 dentine samples/volunteer) and treated once with a SnCl2/AmF/NaF (800 ppm Sn(II), 500 ppm F, pH 4.5) or a NaF solution (500 ppm F, pH 4.5) for 2 min or remained untreated (controls). Samples were eroded with hydrochloric acid (pH 2.6) in a small erosion chamber at 60 microl/min for 25 min. Calcium release into the acid was monitored in consecutive 30s intervals for 5 min, then at 1 min intervals up to a total erosion time of 25 min using the Arsenazo III procedure. Data were statistically analysed by random-effects linear models (p<0.05).
  
  
  RESULTS
  The stannous chloride-containing fluoride solution reduced calcium loss of enamel and dentine to up to 6 min and 3.5 min, respectively. Calcium loss (% of control) amounted from 24+/-7 (30s) up to 93+/-14 (6 min) in enamel and from 38+/-13 (30s) to 87+/-15 (3.5 min) in dentine. The sodium fluoride solution was unable to reduce enamel and dentine erosion at any time point.
  
  
  CONCLUSIONS
  A single application of a stannous chloride-containing fluoride solution reduced enamel and dentine erosion up to 6 min and 3.5 min of constant acid flow, respectively.
authors.affiliation: Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zurich, 8032 Zurich, Switzerland.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Florian Just
authors.initials: FJ
authors.lastname: Wegehaupt
authors.forename: Markus
authors.initials: M
authors.lastname: Zaruba
authors.forename: Klaus
authors.initials: K
authors.lastname: Becker
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Roos
authors.forename: Thomas
authors.initials: T
authors.lastname: Attin
authors.forename: Annette
authors.initials: A
authors.lastname: Wiegand
mesh_terms: D000328:Adult; D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D044622:Dental Pellicle / Q000502:physiology; D003804:Dentin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003959:Diamines / Q000627:therapeutic use*; D005260:Female; D005459:Fluorides / Q000627:therapeutic use*; D005460:Fluorides, Topical / Q000627:therapeutic use*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008297:Male; D012969:Sodium Fluoride / Q000627:therapeutic use; D017971:Tin Compounds / Q000627:therapeutic use*; D014002:Tin Fluorides / Q000627:therapeutic use; D014077:Tooth Erosion / Q000517:prevention & control*
chemical_list: D003959:Diamines; D005460:Fluorides, Topical; D017971:Tin Compounds; D014002:Tin Fluorides; C094808:amine fluoride solution; C023599:stannous chloride; C495399:Olaflur; D012969:Sodium Fluoride; D005459:Fluorides; D002118:Calcium