[Ultrastructure and TUNEL staining on inhibition of Rubus alceaefolius total alkaloids for apoptosis of liver in rat models of acute hepatitis].
OBJECTIVE
To investigate the anti-apoptosis effects of Rubus alceaefolius total alkaloids in rats with hepatic injury.


METHODS
The hepatic injury model of rat was induced by intraperitoneal injection with CCl4. Sixty SD rats were randomly divided into the normal group, the model group, the R. alceaefolius total alkaloids intervened group, and the bifendate intervened group. The expressions of the levels of liver cell apoptosis were determined by TUNEL. Ultrastructures of the liver cells were observed with transmission electron microscope.


RESULTS
Compared with the model group, the degree of hepatic injury and the positive expressions of apoptosis in liver tissues in the R. alceaefolius total alkaloids intervened groups and the bifendate intervened group were significantly lower.


CONCLUSIONS
R. alceaefolius total alkaloids could reduce the pathological changes and degree of hepatic injury in rats, which may be partially through inhibiting the expressions of apoptosis in liver tissue.
Chen
Wenlie
W
Key Laboratory of Basics of Integrative Medicine of Fujian Higher Education, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China. chen.wl@126.com
Hong
Zhenfeng
Z
Li
Tianjiao
T
Zhao
Jinyan
J
Lin
Jiumaol
J
Zhou
Jianheng
J
Huang
Meiyau
M
D000470:Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D006520:Hepatitis, Animal / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019685:Rosales / Q000737:chemistry*
D000470:Alkaloids