Feeder-independent continuous culture of the PICM-19 pig liver stem cell line.
The PICM-19 pig liver stem cell line is a bipotent cell line, i.e., capable of forming either bile ductules or hepatocyte monolayers in vitro, that was derived from the primary culture of pig embryonic stem cells. The cell line has been strictly feeder-dependent in that cell replication, morphology, and function were lost if the cells were cultured without STO feeder cells. A method for the feeder-independent continuous culture of PICM-19 cells (FI-PICM-19) is presented. PICM-19 cells were maintained and grown without feeder cells on collagen I-coated tissue culture plastic for 26 passages (P26) with initial split ratios of 1:3 that diminished to split ratios of less than 1:2 after passage 16. Once plated, the FI-PICM-19 cells were overlaid with a 1:12 to 1:50 dilution of Matrigel or related extracellular matrix product. Growth of the cells was stimulated by daily refeedings with STO feeder-cell conditioned medium. The FI-PICM-19 cells grew to an approximate confluence of 50% prior to each passage at 2-wk intervals. Growth curve analysis showed their average cell number doubling time to be ~96 h. Morphologically, the feeder-independent cells closely resembled PICM-19 cells grown on feeder cells, and biliary canalicui were present at cell-to-cell junctions. However, no spontaneous multicellular ductules formed in the monolayers of FI-PICM-19 cells. Ultrastructural subcellular features of the FI-PICM-19 cells were similar to those of PICM-19 cells cultured on feeder cells. The FI-PICM-19 cells produced a spectrum of serum proteins and expressed many liver/hepatocyte-specific genes. Importantly, cytochrome P450 (EROD) activity, ammonia clearance, and urea production were maintained by the feeder-independent cells. This simple method for the propagation of the PICM-19 cell line without feeder cells should simplify the generation and selection of functional mutants within the population and enhances the cell line's potential for use in toxicological/pharmacological screening assays and for use in an artificial liver device.
Talbot
Neil C
NC
U. S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg. 200, Rm. 13, BARC-East, Beltsville, MD 20705, USA. neil.talbot@ars.usda.gov
Blomberg
Le Ann
Le Ann
Garrett
Wesley M
WM
Caperna
Thomas J
TJ
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003094:Collagen; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004338:Drug Combinations; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000648:ultrastructure*; D007797:Laminin; D008099:Liver / Q000166:cytology*; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D011509:Proteoglycans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014508:Urea / Q000378:metabolism
D015415:Biomarkers; D017931:DNA Primers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D000641:Ammonia; D014508:Urea; D003094:Collagen; D019363:Cytochrome P-450 CYP1A1