title: Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity.
abstract: Ionizing radiation generates oxidative stress, which is thought to be a major cause of aging. Although living organisms are constantly exposed to low levels of radiation, most studies examining the effect of radiation have focused on accelerated aging and diminished life span that result from high-dose radiation. On the other hand, several studies have suggested that low-dose radiation enhances the longevity of Drosophila melanogaster. Therefore, investigation of the biological effects of low-dose radiation could contribute to a more comprehensive understanding of the aging process. In this study, microarray and quantitative real time-PCR were used to measure genome-wide changes in transcript levels in low-dose irradiated fruit flies that showed enhanced longevity. In response to radiation, approximately 13% of the genome exhibited changes in gene expression, and a number of aging-related genes were significantly regulated. These data were compared with quantitative trait loci affecting life-span to identify candidate genes involved in enhanced longevity induced by low-dose radiation. This genome-wide survey revealed novel information about changes in transcript levels in low-dose irradiated flies and identified 39 new candidate genes for molecular markers of extended longevity induced by ionizing radiation. In addition, this study also suggests a mechanism by which low-dose radiation extends longevity.
authors.affiliation: Division of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., Dobong-Gu, Seoul, Korea.
authors.forename: Ki Moon
authors.initials: KM
authors.lastname: Seong
authors.forename: Cha Soon
authors.initials: CS
authors.lastname: Kim
authors.forename: Sun-Won
authors.initials: SW
authors.lastname: Seo
authors.forename: Ha Yeon
authors.initials: HY
authors.lastname: Jeon
authors.forename: Byung-Sub
authors.initials: BS
authors.lastname: Lee
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Nam
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Yang
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Chong Soon
authors.initials: CS
authors.lastname: Kim
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Min
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Jin
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D016678:Genome / Q000528:radiation effects*; D008136:Longevity / Q000235:genetics* / Q000528:radiation effects; D008297:Male; D046228:Microarray Analysis; D018384:Oxidative Stress / Q000235:genetics / Q000528:radiation effects; D016133:Polymerase Chain Reaction / Q000379:methods; D040641:Quantitative Trait Loci; D011839:Radiation, Ionizing