Mcm1p binding sites in the ARG1 promoter positively regulate ARG1 transcription and S. cerevisiae growth in the absence of arginine and Gcn4p.
In this study, we investigated the activating role of Mcm1p at ARG1 during arginine starvation. Our results showed that two Mcm1p binding sites positively contribute to ARG1 transcription and cell growth. Especially, we provide strong evidence that the Mcm1p binding sites play a positive role in ARG1 transcription to overcome arginine starvation in the absence of Gcn4p. In addition, we found that the Mcm1p binding sites are not only regulated by the presence or absence of arginine but also in the presence or absence of other amino acids. These findings suggest that the ARG1 promoter utilizes different DNA elements to control transcription, depending on which amino acids are detected in the medium.
Hong
Suntaek
S
Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon, 406-840, Republic of Korea.
Yoon
Sungpil
S
D001119:Arginase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D015966:Gene Expression Regulation, Fungal; D026341:Minichromosome Maintenance 1 Protein; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
D050976:Basic-Leucine Zipper Transcription Factors; C501391:GCN4 protein, S cerevisiae; C498030:MCM1 protein, S cerevisiae; D026341:Minichromosome Maintenance 1 Protein; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001120:Arginine; C540423:ARG1 protein, S cerevisiae; D001119:Arginase