1,2,3,4,6-penta-O-galloyl-beta-D-glucose protects splenocytes against radiation-induced apoptosis in murine splenocytes.
Antioxidant property and hematopoietic repair capacity are important characteristics of radioprotective agents. Some studies have demonstrated that 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a molecule isolated from the waterlily, has antioxidant, hematopoietic repair, and anti-inflammatory activities. In this study, we try to determine whether PGG extracted from a lily, Nymphaea tetragona var. angusta, has radioprotective effects on splenocytes in vitro against (60)Co gamma-ray irradiation with absorption doses of 2 Gy and 4 Gy. Results show that PGG treatment dramatically enhances the proliferation of splenocytes compared with irradiated but untreated controls. In addition, PGG treatment before irradiation protects the splenocytes from lethal effects of irradiation and decreases DNA damages as identified by the alkaline comet assay. PGG-treated cells also show less radiation-induced apoptosis. These cells have lower concentrations of the pro-apoptotic protein p53 and more of the anti-apoptotic protein Bcl-2. The results presented in this study suggest that PGG has a cytoprotective effect on immune cells exposed to normally damaging amount of radiation. Thus, PGG could be an effective, non-toxic radioprotective agent.
Bing
So Jin
SJ
Department of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, South Korea.
Kim
Min Ju
MJ
Park
Eunjin
E
Ahn
Ginnae
G
Kim
Dae Seung
DS
Ko
Ryeo Kyeong
RK
Lee
Nam Ho
NH
Shin
Taekyun
T
Park
Jae Woo
JW
Jee
Youngheun
Y
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects
D047348:Hydrolyzable Tannins; D017382:Reactive Oxygen Species; C435084:pentagalloylglucose