title: Comparison of conventional ELISA with electrochemiluminescence technology for detection of amyloid-β in plasma.
abstract: Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer's disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.
authors.affiliation: Department of Medicine, Division of Geriatric Medicine and Gerontology, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA. eoh9@jhmi.edu
authors.forename: Esther S
authors.initials: ES
authors.lastname: Oh
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Mielke
authors.forename: Paul B
authors.initials: PB
authors.lastname: Rosenberg
authors.forename: Alka
authors.initials: A
authors.lastname: Jain
authors.forename: Neal S
authors.initials: NS
authors.lastname: Fedarko
authors.forename: Constantine G
authors.initials: CG
authors.lastname: Lyketsos
authors.forename: Pankaj D
authors.initials: PD
authors.lastname: Mehta
mesh_terms: D000544:Alzheimer Disease / Q000097:blood / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000097:blood; D003072:Cognition Disorders / Q000097:blood / Q000175:diagnosis; D055664:Electrochemical Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans
chemical_list: D016229:Amyloid beta-Peptides