Preparation of high-capacity supports containing protein G immobilized to porous silica.
This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39 mg protein G/g silica was obtained when using 100 A pore size silica, followed by 33 mg/g for 50 A silica and 9.3-24 mg/g for 300-4000 A silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037 micromol/m(2) being obtained for 4000 A silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77 mg IgG/g silica) being obtained for 50-100 A silica.
Jackson
Abby J
AJ
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Karle
Elizabeth M
EM
Hage
David S
DS
D000327:Adsorption; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D055767:Immobilized Proteins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D016062:Porosity; D011817:Rabbits; D012545:Schiff Bases / Q000378:metabolism; D012822:Silicon Dioxide / Q000378:metabolism*
C029152:G-substrate; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D012545:Schiff Bases; D012822:Silicon Dioxide