title: Quantification of urinary 8-iso-prostaglandin F2alpha using liquid chromatography-tandem mass spectrometry during cardiac valve surgery.
abstract: Oxidative stress is an unavoidable event during many complex surgical procedures. 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) is a reliable biomarker for the evaluation of oxidative stress in vivo. The aim of this study is to develop simple and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the detection of urinary 8-iso-PGF(2alpha) in samples collected from patients who received a cardiopulmonary bypass (CPB) during cardiac valve surgery. Urine samples of 14 patients with cardiac valve diseases were collected before, during, and after CPB. The level of 8-iso-PGF(2alpha) was detected via selected-reaction monitoring triple quadrupole MS/MS and the result was compared with 12 healthy volunteers. The method's detection limit (3S/N) was 0.25 pg for 8-iso-PGF(2alpha), with a linear working range of 0.25-20 ng/ml. For patients with cardiac valve disease, the 8-iso-PGF(2alpha) levels before the bypasses were the same as those of healthy individuals (P>0.05) and the 8-iso-PGF(2alpha) levels during and after CPB were significantly higher than those before the bypasses (P<0.05). In conclusion, we present a simple and specific protocol for LC-MS/MS quantification of urinary 8-iso-PGF(2alpha) collected during CPB. Using this technique, it would be feasible to assess the levels of oxidative stress during cardiac surgery and thereby helpful for the management of oxidative injury.
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital, Chang Gung University School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Teng
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Wang
authors.forename: Yun-Tsan
authors.initials: YT
authors.lastname: Liao
authors.forename: Min-Wen
authors.initials: MW
authors.lastname: Yang
authors.forename: Tsung-Yun
authors.initials: TY
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006351:Heart Valves / Q000601:surgery*; D006801:Humans; D016014:Linear Models; D008297:Male; D018384:Oxidative Stress; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost