title: FISH mapping of six genes responsible for development of the nervous and skeletal systems on donkey (Equus asinus) chromosomes.
abstract: The results obtained in the present study made it possible to place selected markers responsible for development of the nervous and skeletal systems on the physical map of the donkey genome. Fluorescence in situ hybridization (FISH) was used to localize genes such as GDF5 (15q13), FRZB (4q23.1), TWIST (1q31), PAX6 (20q25), SALL1 (24q15) and SHH (1q35) on donkey chromosomes. The identification of their localization confirmed previously proposed homologies using ZOO-FISH technique, except for FRZB and SALL1 genes. This suggests that they were affected by rearrangements that changed their localization compared to horse, and in the case of the SALL1 gene also compared to human.
authors.affiliation: Department of Immuno and Cytogenetics, National Research Institute of Animal Production, Balice, Poland. mbugno@izoo.krakow.pl
authors.forename: Monika
authors.initials: M
authors.lastname: Bugno-Poniewierska
authors.forename: Klaudia
authors.initials: K
authors.lastname: Pawlina
authors.forename: Aneta
authors.initials: A
authors.lastname: Dardzińska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Zabek
authors.forename: Ewa
authors.initials: E
authors.lastname: Słota
authors.forename: Jolanta
authors.initials: J
authors.lastname: Klukowka-Rötzler
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D018397:Equidae / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics; D005796:Genes / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics; D055428:Growth Differentiation Factor 5 / Q000235:genetics; D053823:Hedgehog Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D041641:Musculoskeletal Development / Q000235:genetics*; D009420:Nervous System / Q000254:growth & development*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D051879:Twist-Related Protein 1 / Q000235:genetics; D000099274:Frizzled-Related Proteins
chemical_list: D005136:Eye Proteins; D006023:Glycoproteins; D055428:Growth Differentiation Factor 5; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D014157:Transcription Factors; D051879:Twist-Related Protein 1; D000099274:Frizzled-Related Proteins; C000605730:PAX6 protein, human