Capillary separation: micellar electrokinetic chromatography.
Micellar electrokinetic chromatography (MEKC), a separation mode of capillary electrophoresis (CE), has enabled the separation of electrically neutral analytes. MEKC can be performed by adding an ionic micelle to the running solution of CE without modifying the instrument. Its separation principle is based on the differential migration of the ionic micelles and the bulk running buffer under electrophoresis conditions and on the interaction between the analyte and the micelle. Hence, MEKC's separation principle is similar to that of chromatography. MEKC is a useful technique particularly for the separation of small molecules, both neutral and charged, and yields high-efficiency separation in a short time with minimum amounts of sample and reagents. To improve the concentration sensitivity of detection, several on-line sample preconcentration techniques such as sweeping have been developed.
Terabe
Shigeru
S
Graduate School of Material Science, University of Hyogo, Kamigori, Hyogo 678-1297, Japan. terabe@gaia.eonet.ne.jp
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000639:trends; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification
D003505:Cyclodextrins; D008823:Micelles; D011108:Polymers; D011506:Proteins