title: Glatiramer acetate-reactive T lymphocytes regulate oligodendrocyte progenitor cell number in vitro: role of IGF-2.
abstract: Glatiramer acetate (GA) is an immunomodulator approved for therapy of relapsing-remitting multiple sclerosis (RRMS), but recent findings indicate that it may also have additional, neurotrophic effects. Here, we found that supernatants from human GA-reactive T lymphocytes potentiated oligodendrocyte numbers in rodent and human oligodendrocyte progenitor (OPC) cultures. Effects of Th2-polarized lines were stronger than Th1-polarized cells. Microarray and ELISA analyses revealed that neurotrophic factors induced in Th2- and Th1-polarized GA-reactive lines included IGF-2 and BMP-7 respectively, and functional studies confirmed IGF-2 as trophic for OPCs. Our results support the concept that GA therapy may result in supportive effects on oligodendrocytes in RRMS patients.
authors.affiliation: Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Jalili
authors.forename: N
authors.initials: N
authors.lastname: Ouamara
authors.forename: A
authors.initials: A
authors.lastname: Zameer
authors.forename: G
authors.initials: G
authors.lastname: Cosentino
authors.forename: M
authors.initials: M
authors.lastname: Mayne
authors.forename: L
authors.initials: L
authors.lastname: Hayardeny
authors.forename: J P
authors.initials: JP
authors.lastname: Antel
authors.forename: A
authors.initials: A
authors.lastname: Bar-Or
authors.forename: G R
authors.initials: GR
authors.lastname: John
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology* / Q000627:therapeutic use; D000818:Animals; D045744:Cell Line, Tumor; D019070:Cell Lineage / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D000068717:Glatiramer Acetate; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000502:physiology*; D018655:Lymphocyte Count / Q000379:methods; D051379:Mice; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010455:Peptides / Q000502:physiology* / Q000627:therapeutic use; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000502:physiology*; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D010455:Peptides; D000068717:Glatiramer Acetate; D007335:Insulin-Like Growth Factor II