High-throughput sequencing of microRNAs in adenovirus type 3 infected human laryngeal epithelial cells.
Adenovirus infection can cause various illnesses depending on the infecting serotype, such as gastroenteritis, conjunctivitis, cystitis, and rash illness, but the infection mechanism is still unknown. MicroRNAs (miRNA) have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3) infected Human laryngeal epithelial (Hep2) cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.
Qi
Yuhua
Y
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, SiPaiLou2#, Nanjing 210096, China.
Tu
Jing
J
Cui
Lunbiao
L
Guo
Xiling
X
Shi
Zhiyang
Z
Li
Shuchun
S
Shi
Wenting
W
Shan
Yunfeng
Y
Ge
Yiyue
Y
Shan
Jun
J
Wang
Hua
H
Lu
Zuhong
Z
D000258:Adenovirus Infections, Human / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D000260:Adenoviruses, Human / Q000502:physiology*; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology / Q000821:virology; D020869:Gene Expression Profiling; D057166:High-Throughput Screening Assays / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D007820:Laryngeal Mucosa / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods; D014779:Virus Replication
D035683:MicroRNAs