Desiccation-induced physiological and biochemical changes in resurrection plant, Selaginella bryopteris.
Selaginella bryopteris is a lycophyte resurrection plant, which incurves during desiccation and recovers on availability of moisture. The aim of the study was to test and understand the various physiological and biochemical changes the fronds undergo during desiccation and rehydration, to get an insight as to how this plant adapts and survives through the dry phase. Upon desiccation, S. bryopteris fronds showed drastic inhibition in net photosynthesis (A) and maximal photochemical efficiency of PSII (F(v)/F(m)) however, chlorophyll content did not show much variation. Dark respiration (R(d)) continued even at 10% relative water content (RWC), and showed a burst after rehydration, which is proposed to be crucial to establish protection mechanisms. Desiccation caused an enhanced production of reactive oxygen species (ROS) and increased lipid peroxidation. Proline accumulation increased substantially by 11-fold. Sucrose and starch contents decreased upon desiccation as compared to control. The antioxidative enzymes viz. superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) along with soluble acid invertase increased during desiccation. S. bryopteris shows mechanical as well as physiological mechanisms for tolerance to extreme levels of desiccation stress. The rapid and almost complete recovery of F(v)/F(m) after rehydration clearly indicates the absence of marked photoinhibitory or thermal injury to PSII during desiccation. This along with the homoiochlorophyllous characteristics enables S. bryopteris to recover its A. The antioxidant metabolism further plays an important role in the desiccation tolerance of S. bryopteris.
Pandey
Vivek
V
Plant Physiology Division, National Botanical Research Institute (NBRI-CSIR), Rana Pratap Marg, Lucknow, Uttar Pradesh 226001, India.
Ranjan
Sanjay
S
Deeba
Farah
F
Pandey
Ashutosh K
AK
Singh
Ruchi
R
Shirke
Pramod A
PA
Pathre
Uday V
UV
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism; D003890:Desiccation; D018506:Gene Expression Regulation, Plant; D010544:Peroxidases / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D032503:Selaginellaceae / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D011392:Proline; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase