title: [Expression of SSB gene in rats with early isehemic myocardium and its implications].
abstract: OBJECTIVE
  To examine the expression of Sjögren's syndrome antigen B (SSB) gene in rats with early ischemic myocardium.
  
  
  METHODS
  Adult healthy Sprague-Dawley rats were randomly divided into four groups (ischemia (MI), non-ischemia (NI), sham-operated (SO) and normal control (NO) groups). The expression of SSB and beta-actin gene in the myocardium was examined by real time PCR at 0 min, 5 min, 15 min, 30 min, 60 min, 120 min after ligation of the left anterior descending coronary artery (LAD).
  
  
  RESULTS
  The expression of SSB gene was down-regulated after ischemia. Significantly lower expression of the gene in the rats with myocardial ischemia was found at 120 min after ligation compared with those before ligation and the control group at 120 min after ligation (P < 0.05 ). However, no significant differences were found between other groups (P > 0.05).
  
  
  CONCLUSIONS
  SSB gene may be involved in the pathophysiologic regulating process in myocardial cells at the early stage of myocardial ischemia in rats.
authors.affiliation: Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Chen
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Gao
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Liang
authors.forename: Yan-Yun
authors.initials: YY
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xue
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Pan
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Fang
authors.forename: Lu-Shun
authors.initials: LS
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D000097585:SS-B Antigen
chemical_list: D000199:Actins; D001324:Autoantigens; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D000097585:SS-B Antigen