Effects of exposure to the xenoestrogen octylphenol and subsequent transfer to clean water on liver and gonad ultrastructure during early development of Zoarces viviparus embryos.
Female eelpouts (Zoarces viviparus L.) are exposed during early pregnancy to nominal concentrations of 100 microg/L of 4-tert-octylphenol (OP) or 0.5 microg/L of 17beta-estradiol (E2). Effects on maternal metabolism and on liver and gonad development in embryos were examined and compared with controls (C) during exposure and after transfer to clean water (depuration). In the mother fish, significantly higher concentrations of plasma vitellogenin (vtg) and calcium were found in the two exposed groups, when compared with the C group after exposure and depuration. When compared, however, with the respective values after exposure, vtg had decreased significantly after depuration. The hepatosomatic index was normalized after depuration. In both exposed groups, the hepatocytes were rounded and not distinctly polygonal as in the controls. The amount of glycogen was considerably less while the number of mitochondria increased, and the rER significantly proliferated after exposure as well as after depuration. The gonads of nine of more than 28 embryos in the group treated with OP exhibited a number of abnormalities as compared with the normal gonad development in both sexes. Feminization of the male gonads in the exposed specimens and a number of histopathological features were observed in all the abnormal gonads, whereas reliable male features, such as formation of seminiferous tubules or spermioduct, were not observed. This study showed that 4t-tert-OP and 17beta-estradiol exert estrogenic effects during very early development of the embryos and that depuration had a positive effect on the motherfish and her embryos.
Jespersen
Ase
A
Institute of Biology, University of Copenhagen, Copenhagen, Denmark. ajespersen@bio.ku.dk
Rasmussen
Tina H
TH
Hirche
Majken
M
Sørensen
Kristine J K
KJ
Korsgaard
Bodil
B
D000818:Animals; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects* / Q000196:embryology; D004781:Environmental Exposure / Q000009:adverse effects; D004958:Estradiol / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005262:Feminization / Q000188:drug therapy / Q000473:pathology; D006066:Gonads / Q000002:abnormalities / Q000187:drug effects* / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects* / Q000196:embryology / Q000648:ultrastructure; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects; D010473:Perciformes / Q000196:embryology / Q000502:physiology*; D010636:Phenols / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D018508:Water Purification
D004968:Estrogens, Non-Steroidal; D010636:Phenols; D014874:Water Pollutants, Chemical; C474055:octylphenol; D004958:Estradiol