Synthesis, characterization, and studies on DNA binding of the complex Fe(Sal(2)dienNO(3).H(2)O).
A new metal complex, Fe(Sal(2)dienNO(3).H2O) (where Sal is salicylaldehyde and dien is diethylenetriamine), has been synthesized and characterized. The interactions between the Fe(III) complex and calf thymus DNA has been investigated using UV and fluorescence spectra, viscosity, thermal denaturation, and molecular modeling. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The experimental results show that the mode of binding of the complex to DNA is classical intercalation and the complex can cleave pBR322 DNA.
Zhou
Cheng-Yong
CY
Department of Chemistry, Changzhi University, Changzhi, Shanxi, 046011, PR China. zcy826@126.com
Wu
Yan-Bo
YB
Yang
Pin
P
D000818:Animals; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004587:Electrophoresis, Agar Gel; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008958:Models, Molecular; D008968:Molecular Conformation; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014783:Viscosity
D056831:Coordination Complexes; D007364:Intercalating Agents; D004247:DNA; C427203:calf thymus DNA