title: Glucose and lactate regulate maitotoxin-activated Ca2+ entry in spermatogenic cells: the role of intracellular [Ca2+].
abstract: Maitotoxin (MTX), a potent polyether marine biotoxin, induces Ca(2+) entry in different mammalian cells by activation of Ca(2+) channels. The identity and modulation of the MTX-activated Ca(2+) entry pathway is not known. In this work, we show, for the first time, that glucose and lactate can modulate the excitability of spermatogenic cell MTX-activated Ca(2+) channels. Physiological and pharmacological evidences indicate that glucose and lactate differentially affect MTX-activated Ca(2+) entry mainly through changes that these substrates induce on intracellular Ca(2+) stores and the concentration of intracellular Ca(2+) ([Ca(2+)](i)) in spermatogenic cells. Our findings strongly suggest that MTX-activated Ca(2+) channels in spermatogenic cells can be regulated by a Ca(2+)-CaM-dependent protein kinase.
authors.affiliation: Instituto de Quimica, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile. jreyes@ucv.cl
authors.forename: Juan G
authors.initials: JG
authors.lastname: Reyes
authors.forename: Nelson
authors.initials: N
authors.lastname: Osses
authors.forename: Marcela
authors.initials: M
authors.lastname: Knox
authors.forename: Alberto
authors.initials: A
authors.lastname: Darszon
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Treviño
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D005854:Germ Cells / Q000378:metabolism; D005947:Glucose; D007477:Ions / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D008387:Marine Toxins; D035964:Oxocins; D051381:Rats; D017207:Rats, Sprague-Dawley; D000095702:Polyether Toxins
chemical_list: D002118:Calcium; D005947:Glucose; D007477:Ions; D019344:Lactic Acid; D008387:Marine Toxins; D035964:Oxocins; C020394:maitotoxin; D000095702:Polyether Toxins