title: The Ontario universal typing of tuberculosis (OUT-TB) surveillance program--what it means to you.
abstract: BACKGROUND
  Tuberculosis (TB) is a serious disease that is transmitted primarily by the airborne route. Effective disease control and outbreak management requires the timely diagnosis, isolation and treatment of infected individuals with active disease; contact tracing to identify secondary cases likely to benefit from treatment of latent infection; and laboratory identification or confirmation of epidemiologically linked cases. TB genotyping enables the comparison of Mycobacterium tuberculosis complex (MTBC) strains and the identification of cases that may or may not be linked. The increased availability of molecular methods for genotyping has allowed for greater discrimination of MTBC strains and greatly enhanced understanding of TB transmission patterns.
  
  
  OBJECTIVE
  To improve TB surveillance and control in Ontario, the Public Health Laboratories of the Ontario Agency for Health Protection and Promotion has introduced the Ontario Universal Typing of Tuberculosis (OUT-TB) Surveillance Program.
  
  
  METHODS
  The first isolate from every new TB case will be genotyped with two rapid molecular methods: spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing. MTBC isolates with nonunique genotypes and, thus, potentially linked to other TB cases, will also be genotyped by IS6110 restriction fragment length polymorphism analysis.
  
  
  CONCLUSIONS
  By providing TB control programs using these new genotyping tools, and using traditional and new case investigation methods (eg, social network analysis), this new program will provide a clearer picture of TB in Ontario, and permit more effective use of public health resources and improve disease control.
authors.affiliation: Public Health Laboratory, Toronto, Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada.
authors.forename: Shelly
authors.initials: S
authors.lastname: Bolotin
authors.forename: David C
authors.initials: DC
authors.lastname: Alexander
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Guthrie
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Drews
authors.forename: Frances
authors.initials: F
authors.lastname: Jamieson
mesh_terms: D015373:Bacterial Typing Techniques; D005838:Genotype; D006801:Humans; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*; D009864:Ontario; D011159:Population Surveillance / Q000379:methods*; D014376:Tuberculosis / Q000382:microbiology* / Q000517:prevention & control