Antiviral effect of PmRab7 knock-down on inhibition of Laem-Singh virus replication in black tiger shrimp.
PmRab7 is a Penaeus monodon small GTPase protein possibly involved in replication of several shrimp viruses. In this study RNA interference (RNAi) using double-stranded RNA (dsRNA) targeting PmRab7 gene (dsRNA-PmRab7) was employed to silence the expression of PmRab7 to investigate the inhibitory effect on Laem-Singh virus (LSNV) replication. Injection of dsRNA-PmRab7 24h before challenge with the virus resulted in a drastic decrease of PmRab7 mRNA and complete inhibition of LSNV replication at 3 days post-challenge. In a therapeutic mode, shrimp injected with dsRNA-PmRab7 1 day but not at 3 or 5 days post-LSNV challenge resulted in inhibition of LSNV replication. These results pave the way to use dsRNA-PmRab7 to prevent or cure LSNV infection in shrimp.
Ongvarrasopone
Chalermporn
C
Institute of Molecular Biosciences, Mahidol University (Salaya Campus), 25/25 Phutthamonthon 4 Rd., Salaya, Phutthamonthon District, Nakhon Pathom 73170, Thailand. mbcov@mahidol.ac.th
Chomchay
Ekapol
E
Panyim
Sakol
S
D000818:Animals; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D033561:Penaeidae / Q000821:virology*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012328:RNA Viruses / Q000302:isolation & purification / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012758:Shellfish / Q000821:virology; D014779:Virus Replication; D020691:rab GTP-Binding Proteins / Q000235:genetics*; D000090802:rab7 GTP-Binding Proteins
D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins