title: Antiviral effect of strictinin on influenza virus replication.
abstract: Strictinin, which is a member of the ellagitanin family of hydrolyzable tannins, prevented replication of human, duck and swine influenza A viruses (IAVs) in vitro at non-toxic concentrations. The addition of strictinin at the same time as IAV inoculation to MDCK cells inhibited viral replication in a dose-dependent manner. Strictinin showed 50% inhibitory concentrations for IAVs from 0.09±0.021 to 0.28±0.037μM (mean±S.E.M.) by the focus-forming assay. Treatment of MDCK cells with strictinin before and after viral inoculation resulted in no significant antiviral activity. Further studies showed that strictinin inhibited IAV-induced hemifusion. However, strictinin exhibited no inhibitory effect against receptor binding, sialidase activity. Strictinin also showed an antiviral effect on influenza B virus and human parainfluenza virus type-1 in vitro. The results indicate that strictinin is a useful antiviral agent.
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
authors.forename: Repon Kumer
authors.initials: RK
authors.lastname: Saha
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Kurebayashi
authors.forename: Keijo
authors.initials: K
authors.lastname: Fukushima
authors.forename: Akira
authors.initials: A
authors.lastname: Minami
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kinbara
authors.forename: Masaki
authors.initials: M
authors.lastname: Ichitani
authors.forename: Yuko M
authors.initials: YM
authors.lastname: Sagesaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D009981:Influenza B virus / Q000187:drug effects* / Q000502:physiology; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology; D053585:Virus Attachment / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010636:Phenols; D013634:Tannins; C496786:strictinin; D009439:Neuraminidase