[Podocyte injury and expression of nephrin and VEGF in rat nephrosis model induced by adriamycin].
OBJECTIVE
To investigate podocyte injury and the expression of nephrin and VEGF in rat nephrosis model induced by adriamycin.


METHODS
The rat adriamycin induced nephrosis model was established, while the biochemical indicators in blood and urine were measured and the pathological changes of the renal tissue were evaluated by light microscope and electron microscope. The podocyte number was counted, and the expression levels of nephrin, VEGF were examined at different time by means of immunohistochemistry.


RESULTS
After second injected with adriamycin,the model group nephrin presented a weak signal in the end of the first week (P < 0.05), and the expression of VEGF started to increase at the end of the eighth week (P < 0.05). The podocyte number decreased at the end of the eighth week (P < 0.05). The expression of nephrin and the number of podocyte were negatively correlated with the 24-hour urine protein, blood urea nitrogen and serum creatinine; while the expression of VEGF was positively correlated with the 24-hour urine protein, blood urea nitrogen and serum creatinine.


CONCLUSIONS
The decrease of nephrin expression and the change of its distribution might be the significant factors resulting in considerable proteinuria. VEGF participated in the process of proteinuria and glomerular sclerosis in the development of rat adriamycin nephrosis.
Yang
Wei-Na
WN
Department of Human Anatomy, Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
Ren
Shu-Ting
ST
Qian
Yi-Hua
YH
Cheng
Shao-Li
SL
Jin
Hui
H
Liu
Yong
Y
Yu
Lin-Hua
LH
D000818:Animals; D004317:Doxorubicin; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009401:Nephrosis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D050199:Podocytes / Q000473:pathology / Q000648:ultrastructure*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; D042461:Vascular Endothelial Growth Factor A; C113267:nephrin; C467498:vascular endothelial growth factor A, rat; D004317:Doxorubicin