title: Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads.
abstract: For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Keio University, 1-5-14 Shibakoen, Minato, Tokyo 105-0011, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Mizutani
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nagase
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Hideko
authors.initials: H
authors.lastname: Kanazawa
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Annaka
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000809:Angiotensins / Q000737:chemistry / Q000302:isolation & purification; D001709:Biotechnology / Q000379:methods*; D001920:Bradykinin / Q000737:chemistry / Q000302:isolation & purification; D008863:Microspheres; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D000809:Angiotensins; D010455:Peptides; D011108:Polymers; D011137:Polystyrenes; C052970:poly-N-isopropylacrylamide; D001920:Bradykinin