NLS-mediated NPC functions of the nucleoporin Pom121.
RanGTP mediates nuclear import and mitotic spindle assembly by dissociating import receptors from nuclear localization signal (NLS) bearing proteins. We investigated the interplay between import receptors and the transmembrane nucleoporin Pom121. We found that Pom121 interacts with importin alpha/beta and a group of nucleoporins in an NLS-dependent manner. In vivo, replacement of Pom121 with an NLS mutant version resulted in defective nuclear transport, induction of aberrant cytoplasmic membrane stacks and decreased cell viability. We propose that the NLS sites of Pom121 affect its function in NPC assembly both by influencing nucleoporin interactions and pore membrane structure.
Yavuz
Sevil
S
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Santarella-Mellwig
Rachel
R
Koch
Birgit
B
Jaedicke
Andreas
A
Mattaj
Iain W
IW
Antonin
Wolfram
W
D000818:Animals; D002470:Cell Survival; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009685:Nuclear Envelope / Q000378:metabolism / Q000648:ultrastructure; D019913:Nuclear Localization Signals / Q000378:metabolism*; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D013329:Structure-Activity Relationship; D014981:Xenopus; D029867:Xenopus Proteins / Q000378:metabolism*; D028961:beta Karyopherins / Q000378:metabolism
D008562:Membrane Glycoproteins; D050505:Mutant Proteins; D019913:Nuclear Localization Signals; D028861:Nuclear Pore Complex Proteins; C094157:POM121 protein, human; C552113:Pom121 protein, Xenopus; D029867:Xenopus Proteins; D028961:beta Karyopherins