Selenium partially reverses the depletion of striatal dopamine and its metabolites in MPTP-treated C57BL mice.
Oxidative stress and inflammation have been implicated in idiopathic Parkinson's disease as well as in the mouse model of this disorder induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Selenium possesses both antioxidant and anti-inflammatory properties; thus we studied the effect of selenium supplementation on MPTP-induced dopaminergic neurotoxicity in mice. C57BL male mice were treated with MPTP (30 mg/kg, i.p.), daily for 4 days. Sodium selenite (Se) was administered in the doses of 0, 1, 2 and 3 mg/kg, 30 min prior to the administration of MPTP. One group of animals served as control (saline only) and another group as Se alone (3 mg/kg). The animals were sacrificed at 24 h after the last dose of MPTP. The striata were isolated and analyzed for dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) levels. Administration of MPTP significantly depleted striatal DA (6.78+/-0.80 microg/g) as compared to control animals (19.32+/-0.77 microg/g) which was significantly prevented by co-treatment with 3 mg/kg dose of Se (12.28+/-0.97 microg/g). MPTP caused significant reduction in striatal DOPAC but the decrease in HVA levels was not significant. Although Se dose-dependently reversed MPTP-induced decreases in DOPAC and HVA levels, these effects were statistically not significant. These findings indicate a significant impairment of dopaminergic neurotransmission by MPTP which is partially reversed by Se treatment.
Khan
Haseeb Ahmad
HA
Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia. khan_haseeb@yahoo.com
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D006719:Homovanillic Acid / Q000378:metabolism; D020267:MPTP Poisoning / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018036:Selenium Compounds / Q000494:pharmacology*
D018036:Selenium Compounds; D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid