title: Nukacin 3299, a lantibiotic produced by Staphylococcus simulans 3299 identical to nukacin ISK-1.
abstract: Nukacin 3299 (formerly designated simulancin 3299), produced by a Staphylococcus simulans strain involved in bovine mastitis in Brazil, is the first peptide bacteriocin described in this staphylococcal species. With the intent to elucidate some aspects of its biology, nukacin 3299 was purified and characterized. The mass of the purified bacteriocin was shown to be 2957.3 Da, and the peptide N-terminal amino acids (KKKSGVI) were identified by Edman degradation. The nukacin 3299 structural gene, nukA, was detected by PCR and DNA sequencing, showing that this bacteriocin is identical to nukacin ISK-1, a 27-amino acid type-A (II) lantibiotic produced by Staphylococcus warneri ISK-1, isolated from a "nukadoko", in Japan. The genes involved in nukacin 3299 biosynthesis are located on plasmid pRJ97 (>27 kb). They have an organization similar to that of the nukacin ISK-1 gene cluster, excepted for the presence of an IS257/431 element (791 bp) present between the orf1 and nukA genes of the nukacin 3299 gene cluster. The presence of this insertion sequence is expected to affect the expression of orf1, whose function is presently unknown. Nukacin 3299 proved to be sensitive to proteolytic enzymes and relatively stable at different temperatures and between pH 3.0-9.0. Nukacin 3299 exhibited activity towards staphylococcal strains involved in bovine mastitis, showing a potential application on mastitis control, a disease with great economic impact.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Hilana
authors.initials: H
authors.lastname: Ceotto
authors.forename: Helge
authors.initials: H
authors.lastname: Holo
authors.forename: Karlla Fernanda Silva
authors.initials: KF
authors.lastname: da Costa
authors.forename: Janaína dos Santos
authors.initials: Jdos S
authors.lastname: Nascimento
authors.forename: Zhian
authors.initials: Z
authors.lastname: Salehian
authors.forename: Ingolf F
authors.initials: IF
authors.lastname: Nes
authors.forename: Maria do Carmo de Freire
authors.initials: Mdo C
authors.lastname: Bastos
mesh_terms: D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D008414:Mastitis, Bovine / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000378:metabolism*
chemical_list: D001430:Bacteriocins; C421186:nukacin ISK-1