New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay.
Several analytical approaches are available for investigating the antioxidant power for antioxidants, and they are based on a variety of chemical principles, such as oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing/antioxidant power (FRAP). This paper reports a new rapid method for investigating antioxidant power on the basis of the electron-donating ability. This method is called chemiluminescence analysis of antioxidant power (CAAP). The electrons donated from antioxidants are capable of inducing chemiluminescence in the presence of lucigenin and a base. Thus, the intensity of chemiluminescence induced by antioxidants is proportional to their electron-donating ability (antioxidant power). It was found that the correlation between CAAP and FRAP was positive (r = 0.959) and statistically significant (p < 0.05). In addition to the FRAP assay, the rapid CAAP assay is convenient for investigating the antioxidant power of herbal extracts.
Chen
Tung-Sheng
TS
Graduate Institute of Basic Medical Science, China Medical University; Department of Health and Nutrition Biotechnology, Asia University, Taichung 404, Taiwan.
Liou
Show-Yih
SY
Wu
Hsi-Chin
HC
Tsai
Fuu-Jen
FJ
Tsai
Chang-Hai
CH
Huang
Chih-Yang
CY
Chang
Yen-Lin
YL
D000975:Antioxidants / Q000737:chemistry*; D028244:Camellia / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002839:Chromans / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry; D027843:Vitis / Q000737:chemistry
D000975:Antioxidants; D002839:Chromans; D005290:Ferric Compounds; D010936:Plant Extracts; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid