[Quantitative expression of voltage-dependent calcium channels in prostate smooth muscle cells of rats with estradiol-induced chronic nonbacterial prostatitis].
OBJECTIVE
To construct a rat model of chronic nonbacterial prostatitis (CP) and investigate the difference in the quantitative expression of voltage-dependent calcium channels of prostate smooth muscle cells (PSMCs) between the models and controls.


METHODS
We established a CP rat model by estrogen induction, cultured and purified the PSMCs in vitro, and extracted total RNA by Trizol. Then we measured the mRNA expression of the cal subunit in the calcium channel subtypes by reverse transcription and SYBR Green I real time RT-PCR, and compared it with that of the controls.


RESULTS
The expressions of the L-, T- and P/Q-type calcium channels were found in both the CP and control groups, and that of the CaV1.2 L-type calcium channel was significantly increased in the former as compared with the latter (0.048 +/- 0.024 versus 0.031 +/- 0.015, t = 2.846, P = 0.007), but there were no statistically significant differences in the T- and P/Q-type calcium channels between the two groups.


CONCLUSIONS
The number of CaV1.2 L-type calcium channels of PSMCs and calcium influx were increased in CP patients, which may be involved in the mechanism of CP.
Zhang
Liang
L
Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. zlcommandos2@yahoo.com.cn
Liang
Chao-Zhao
CZ
Zhang
Xian-Sheng
XS
Hao
Zong-Yao
ZY
Zhou
Jun
J
Fan
Song
S
Li
Yu
Y
D000818:Animals; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020907:Calcium Channels, Q-Type / Q000378:metabolism*; D020747:Calcium Channels, T-Type / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism; D011472:Prostatitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D020746:Calcium Channels, L-Type; D020907:Calcium Channels, Q-Type; D020747:Calcium Channels, T-Type; C419385:L-type calcium channel alpha(1C); D012333:RNA, Messenger; D004958:Estradiol