title: Assessing bioavailability of the solubilization of organic compound in nonionic surfactant micelles by dose-response analysis.
abstract: It is uncertain in some extent that organic compounds solubilized in micelles of a nonionic surfactant aqueous solution are bioavailable directly by the microbes in an extractive microbial transformation or biodegradation process. In this work, a dose-response method, where a bioequivalence concept is introduced to evaluate the synergic toxicity of the nonionic surfactants and the organic compounds, was applied to analyze the inhibition effect of organic compounds (naphthalene, phenyl ether, 2-phenylethanol, and 1-butanol) in nonionic surfactant Triton X-100 micelle aqueous solutions and Triton X-114 in aqueous solutions forming cloud point systems. Based on the result, a mole solubilization ratio of organic compounds in micelle was also determined, which consisted very well with those of classic semi-equilibrium dialysis experiments. The results exhibit that bioavailability of organic compounds solubilized in micelles to microbial cells is negligible, which provides a guideline for application of nonionic surfactant micelle aqueous solutions or cloud point systems as novel media for microbial transformations or biodegradations.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Dai
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
authors.forename: Hanshi
authors.initials: H
authors.lastname: Qi
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D001682:Biological Availability; D003470:Culture Media / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D008823:Micelles; D009930:Organic Chemicals / Q000493:pharmacokinetics* / Q000633:toxicity*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D003470:Culture Media; D004337:Drug Carriers; D008823:Micelles; D009930:Organic Chemicals; D013501:Surface-Active Agents