title: The metabolic network of Synechocystis sp. PCC 6803: systemic properties of autotrophic growth.
abstract: Unicellular cyanobacteria have attracted growing attention as potential host organisms for the production of valuable organic products and provide an ideal model to understand oxygenic photosynthesis and phototrophic metabolism. To obtain insight into the functional properties of phototrophic growth, we present a detailed reconstruction of the primary metabolic network of the autotrophic prokaryote Synechocystis sp. PCC 6803. The reconstruction is based on multiple data sources and extensive manual curation and significantly extends currently available repositories of cyanobacterial metabolism. A systematic functional analysis, utilizing the framework of flux-balance analysis, allows the prediction of essential metabolic pathways and reactions and allows the identification of inconsistencies in the current annotation. As a counterintuitive result, our computational model indicates that photorespiration is beneficial to achieve optimal growth rates. The reconstruction process highlights several obstacles currently encountered in the context of large-scale reconstructions of metabolic networks.
authors.affiliation: Institute for Theoretical Biology, Humboldt University Berlin, Berlin, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Knoop
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Zilliges
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lockau
authors.forename: Ralf
authors.initials: R
authors.lastname: Steuer
mesh_terms: D052818:Autotrophic Processes / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism; D019069:Cell Respiration; D017353:Gene Deletion; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D052817:Phototrophic Processes; D046939:Synechocystis / Q000166:cytology / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide