Anti-oxidant effects of resveratrol on mice with DSS-induced ulcerative colitis.
OBJECTIVE
Oxidant/antioxidant balance is suggested to be an important factor for the recurrence and progression of ulcerative colitis (UC). The aim of the study is to investigate the potential protective role of resveratrol (Res) against dextran sodium sulfate (DSS)-induced oxidative damage in colon of mice with UC.


METHODS
UC was induced in mice by oral administration of synthetic DSS (molecular weight 5000) for 7 days. Mice were divided into normal group, colitis control group, low-dose Res-treated group (RLD-treated group), and high-dose Res-treated group (RHD-treated group). Inhibitory effects of concomitant treatment with Res were assessed daily using a Disease Activity Index (DAI) and severity of histological changes. MDA, MPO, SOD and GSH-PX activity of colonic tissue were determined in colon samples by chemical colorimetry. TNF-alpha, IL-8, IFN-gamma, p22(phox) and gp91(phox) expression levels were detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), ELISA, and Western blot analysis.


RESULTS
Administration of Res significantly inhibited the severity of UC compared to the colitis control group. Colonic tissue MDA and MPO activities decreased significantly in Res-treated groups compared to colitis control groups. Furthermore, colonic tissue SOD and GSH-Px activities increased significantly in Res-treated groups compared to colitis control groups. The expression levels of TNF-alpha, IL-8, IFN-gamma, p22(phox), and gp91(phox) also decreased significantly in the Res-treated group compared to the colitis control group.


CONCLUSIONS
Oral administration of Res exerts marked inhibitory effects on UC in mice. Resveratrol may play an important role in preventing DSS-induced oxidative damage.
Yao
Jun
J
Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China.
Wang
Jian-Yao
JY
Liu
Lei
L
Li
Ying-Xue
YX
Xun
An-Ying
AY
Zeng
Wei-Sen
WS
Jia
Chun-Hong
CH
Wei
Xiao-Xia
XX
Feng
Ju-Ling
JL
Zhao
Li
L
Wang
Li-Sheng
LS
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001483:Base Sequence; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D003106:Colon / Q000201:enzymology / Q000378:metabolism; D017931:DNA Primers; D016264:Dextran Sulfate / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D017931:DNA Primers; D017382:Reactive Oxygen Species; D013267:Stilbenes; D008315:Malondialdehyde; D016264:Dextran Sulfate; D009195:Peroxidase; D005979:Glutathione Peroxidase; D000077185:Resveratrol