title: Imaging targeted-agent binding in vivo with two probes.
abstract: An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3 x 10(-5) s(-1)), as compared to the normal pancreas (3.4 x 10(-5) s(-1)). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
authors.forename: Kimberley S
authors.initials: KS
authors.lastname: Samkoe
authors.forename: Shannon
authors.initials: S
authors.lastname: Hextrum
authors.forename: Julia A
authors.initials: JA
authors.lastname: O'Hara
authors.forename: Michael
authors.initials: M
authors.lastname: Jermyn
authors.forename: Subhadra
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Hasan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007700:Kinetics; D016014:Linear Models; D051379:Mice; D008954:Models, Biological; D010190:Pancreatic Neoplasms / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014183:Transplantation, Heterologous; D051598:Whole Body Imaging
chemical_list: D005456:Fluorescent Dyes; D004815:Epidermal Growth Factor