Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system.
In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae.
Suehiro
Yuji
Y
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Kinoshita
Masato
M
Okuyama
Teruhiro
T
Shimada
Atsuko
A
Naruse
Kiyoshi
K
Takeda
Hiroyuki
H
Kubo
Takeo
T
Hashimoto
Mitsuhiro
M
Takeuchi
Hideaki
H
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism* / Q000821:virology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D019426:Integrases; D008164:Luminescent Proteins / Q000235:genetics; D009990:Oryzias / Q000033:anatomy & histology / Q000235:genetics* / Q000821:virology*; D011994:Recombinant Proteins / Q000235:genetics
D008164:Luminescent Proteins; D011994:Recombinant Proteins; C413663:fluorescent protein 583; C045073:Cre recombinase; D019426:Integrases