title: Expression of the IP3R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum.
abstract: The cerebellar Purkinje cell monolayer is organized into heterogeneous Purkinje cell compartments that have different molecular compositions. Here we describe a transgenic mouse line, 1NM13, that shows heterogeneous transgene expression in parasagittal Purkinje cell arrays. The transgene consists of a nuclear localization signal (nls) fused to the beta-galactosidase (lacZ) composite gene driven by the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1) gene promoter. IP(3)R1-nls-lacZ transgene expression was detected at a single Purkinje cell level over the surface of a whole-mount X-gal-stained cerebellum because of nuclear accumulation of the nls-lacZ activity. Developing cerebella of 1NM13 mice showed stripe-like X-gal staining patterns of parasagittal Purkinje cell subsets. The X-gal stripe pattern was likely determined by an intrinsic property as early as E15 and showed increasing complexity with cerebellar development. The X-gal stripe pattern was reminiscent of, but not identical to, the stripe pattern of zebrin II immunoreactivity. We designated the symmetrical X-gal-positive (transgene-positive, Tg(+)) Purkinje cell stripes about the midline as vermal Tg1(+), Tg2(a, b)(+) and Tg3(a, b)(+) stripes and hemispheric Tg4(a, b)(+), Tg5(a, b)(+), Tg6(a, b, c)(+), and Tg7(a, b)(+) stripes, where a, b, and c indicate substripes. We also assigned three parafloccular substripes Tg8(a, b, c)(+). The boundaries of X-gal stripes at P5 were consistent with raphes in the Purkinje cell layer through which granule cells migrate, suggesting a possible association of the X-gal stripes with raphe formation. Our results indicate that 1NM13 is a good mouse model with a reproducible and clear marker for the compartmentalization of Purkinje cell arrays.
authors.affiliation: First Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Furutama
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Morita
authors.forename: Riya
authors.initials: R
authors.lastname: Takano
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sekine
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Sadakata
authors.forename: Yo
authors.initials: Y
authors.lastname: Shinoda
authors.forename: Kanehiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Mishima
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Mikoshiba
authors.forename: Richard
authors.initials: R
authors.lastname: Hawkes
authors.forename: Teiichi
authors.initials: T
authors.lastname: Furuichi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D002794:Choline / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D019913:Nuclear Localization Signals / Q000235:genetics*; D009924:Organ Culture Techniques; D011401:Promoter Regions, Genetic / Q000235:genetics; D011689:Purkinje Cells / Q000502:physiology*
chemical_list: C506926:ITPR1 protein, human; D053496:Inositol 1,4,5-Trisphosphate Receptors; D009419:Nerve Tissue Proteins; D019913:Nuclear Localization Signals; C071871:zebrin II; C008561:murexine; D002794:Choline