Alleviation of doxorubicin-induced toxicities by anthocyanin-rich bilberry (Vaccinium myrtillus L.) extract in rats and mice.
The objective of this study was to investigate the effects of anthocyanin-rich bilberry extract (BE) with highly antioxidative potential against doxorubicin (Dox)-induced toxicity in rat and mouse models. Sprague-Dawley rats treated with Dox (15 mg/kg intraperitoneally) showed marked body weight loss, increased abdominal ascites and serum glutamate oxaloacetate transaminase (GOT) level, serum and cardiac lipid peroxidation, myocardial histopathological damage, and depletion of cardiac glutathione (GSH). Dietary supplementation with 1% BE significantly reduced serum lipid peroxidation and increased cardiac creatine phosphokinase activity and total GSH level compared with the levels in the Dox control rats (P < 0.05). Serum GOT and cardiac lipid peroxide levels did not change significantly after BE treatment. Morphologic examination revealed that Dox-induced myocardial damage was also significantly suppressed in rats fed with the 1% BE diet. Oral administration of 500 mg/kg of BE for 10 days to mice treated with Dox (10 mg/kg) partially restored the Dox-induced changes by increasing red blood cell and bone marrow cell counts, and hemoglobin level. Although the protective effects of BE were insufficient to completely counteract the toxic effects of Dox, these results suggest that BE supplementation provides moderate protection against Dox-induced cardiac and hematopoietic damage.
Choi
Eun Hye
EH
Food Safety Research Center, Korea Food Research Institute, Bundang-gu, Sungnam-si, Kyonggi-do, South Korea.
Park
Jae Hwan
JH
Kim
Mi Kyung
MK
Chun
Hyang Sook
HS
D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D004317:Doxorubicin / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts; D051381:Rats; D017207:Rats, Sprague-Dawley; D029800:Vaccinium myrtillus
D000872:Anthocyanins; D010936:Plant Extracts; D004317:Doxorubicin; C073305:Vaccinium myrtillus extract