Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs.
Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture.
Suphaphiphat
Pirada
P
Novartis Vaccines and Diagnostics, Cambridge, MA, USA.
Franti
Michael
M
Hekele
Armin
A
Lilja
Anders
A
Spencer
Terika
T
Settembre
Ethan
E
Palmer
Gene
G
Crotta
Stefania
S
Tuccino
Annunziata B
AB
Keiner
Bjoern
B
Trusheim
Heidi
H
Balabanis
Kara
K
Sackal
Melissa
M
Rothfeder
Mithra
M
Mandl
Christian W
CW
Dormitzer
Philip R
PR
Mason
Peter W
PW
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004196:Disease Outbreaks; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016415:Sequence Alignment; D014779:Virus Replication
C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus