[Effects of ADAM28 on biological functions of human dental pulp stem cells].
OBJECTIVE
To investigate the effects of a disintegrin and metalloproteinase 28 (ADAM28) on proliferation, differentiation and apoptosis of human dental pulp stem cells (HDPSCs) and the possible mechanism.


METHODS
Firstly, HDPSCs were isolated and cultured in vitro and identified. ADAM28 eukaryotic expression plasmid was constructed via gene rebuilt technique and transfected into HDPSCs. Then MTT chromatometry, enzyme dynamics and flow cytometry (FCM) techniques were performed to detect the effects of ADAM28 on biological characteristics of HDPSCs. Immunocytochemical and image analysis techniques were used to determine the influence of ADAM28 on HDPSCs expressing dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP) and osteopontin (OPN). Statistical significance was assessed by the Student-Newman-Keuls (SNK) test with SPSS 13.0 software package.


RESULTS
ADAM28 eukaryotic plasmid was constructed and transfected into HDPSCs for 48 hours successfully. In ADAM28 eukaryotic plasmid group, proliferation activity and index of HDPSCs were lower than those of pcDNA3.1(+) group and untransfected group significantly.Alkaline phosphatase (ALP) secretion level and percentage of apoptotic cells went up remarkly. Significant difference was detected between eukaryotic plasmid group and other groups (P<0.05). The expression level of DSPP in HDPSCs elevated significantly (P<0.05).


CONCLUSIONS
ADAM28 could inhibit HDPSCs proliferation, promote ALP secretion activity and DSPP expression in HDPSCs and induce HDPSCs apoptosis significantly.
Zhao
Zheng
Z
Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China. zhaozheng170@sohu.com
Liu
Hong-chen
HC
E
Ling-ling
LL
Wang
Yi
Y
Wang
Dong-sheng
DS
D051722:ADAM Proteins; D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D003782:Dental Pulp; D016326:Extracellular Matrix Proteins; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D010750:Phosphoproteins; D010957:Plasmids; D012795:Sialoglycoproteins; D013234:Stem Cells; D014162:Transfection; D000099311:Dentin Sialophosphoprotein
D051722:ADAM Proteins; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D053495:Osteopontin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C410224:ADAM28 protein, human