Chiral separation of diastereoisomers of cefuroxime axetil by high-performance thin-layer chromatography.
Conditions for separation and determination of diastereoisomers of cefuroxime axetil by HPTLC on cellulose layers with densitometry were developed. A good separation of the constituents was achieved using a mobile phase composed of 1% aqueous beta-cyclodextrin-methanol (15 + 1, v/v). For detecting spots on the chromatograms, densitometric measurements were used at 285 nm. The method is characterized by high sensitivity; the LOD was 0.04 microg/spot and LOQ was 0.11 microg/spot for both forms of the cefuroxime axetil diastereoisomers. For the individual diastereoisomers, high recovery values from 96.63 to 104.16% were obtained. Identification of the diastereoisomers was performed by 1H-NMR spectrometric analysis.
Dabrowska
Monika
M
Jagiellonian University, Collegium Medicum, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688 Kraków, Poland.
Krzek
Jan
J
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002444:Cefuroxime / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013237:Stereoisomerism
D000900:Anti-Bacterial Agents; D002444:Cefuroxime; C040738:cefuroxime axetil