Development of an efficient and simple method for conjugation of laccase to immunoglobulin and its characterization by enzyme immunoassay.
A new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi Pleurotus ostreatus. The efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. The assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody.
Ray
Swarnadeep
S
Department of Physiology, Immunology Laboratory, University of Calcutta, Calcutta, India.
Chowdhury
Protik
P
Das
Nirmalendu
N
Bishayi
Biswadev
B
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002417:Cattle; D005976:Glutaral; D007124:Immunoenzyme Techniques / Q000379:methods*; D007136:Immunoglobulins / Q000737:chemistry* / Q000276:immunology*; D042845:Laccase / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D010504:Periodic Acid; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology
D000906:Antibodies; D007136:Immunoglobulins; D010504:Periodic Acid; D012710:Serum Albumin, Bovine; C009288:metaperiodate; D042845:Laccase; D009195:Peroxidase; D005976:Glutaral