[Experimental study of calcium phosphate cement mixed with bFGF and VEGF to repair bone defects in rabbit radius].
OBJECTIVE
To explore the synergistic effect of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) on the growth of bone tissue.


METHODS
A total of 36 rabbits were randomly divided into 4 groups with 9 rabbits (18 sides of the anterior limb) in each group, including group A,B,C, and D. For all rabbits 1.0 cm bone defects was created in both sides of the radius. These bone defect regions were implanted with corresponding composites: group A with calcium phosphate cement (CPC) only, group B with CPC/bFGF, group C with CPC/VEGF while group D with both bFGF and VEGF. At the 3rd, 6th, and 12th week after the operation, 6 specimens from each group were randomly selected. The effects were partly assessed by X-ray film examination, bone mineral density (BMD) measurements, biomechanical test and histological observation.


RESULTS
X-ray showed that at the 12th week the bone defects in group D were completely repaired with CPC generally degraded,whereas bone defects in group B and C were only basically repaired. BMD measurements showed that at the 12th week the BMD of group D was significantly higher than that of group B and C (P < 0.05). Biomechanical testing(at the 12th week) showed that the maximum bending load of group D was significantly higher than that of group B and C (P < 0.05). Histological observation indicated that at the 12th week, woven bone had become mature lamellar bone in group D. At the same time, the normal relation of cortical bone and marrow had resumed, and so had the normal structure of trabecula. However, the recanalization of bone marrow cavity could not be seen in group B and C.


CONCLUSIONS
These 3 kinds of composite: CPC/bFGF, CPC/VEGF and CPC/ bFGF+VEGF can promote the growth of bone tissue and speed up the repair of bone defects. The composite of CPC/bFGF+VEGF is better than the other two composites in promoting the growth of bone tissues, indicating that bFGF and VEGF have a synergistic effect on the growth of bone tissue.
Xie
Qiu'en
Q
Department of Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
He
Aiyong
A
Song
Kunxiu
K
D000818:Animals; D001843:Bone Cements; D002130:Calcium Phosphates / Q000008:administration & dosage*; D004357:Drug Synergism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D020007:Implants, Experimental; D008297:Male; D011817:Rabbits; D011884:Radius / Q000293:injuries* / Q000601:surgery; D011897:Random Allocation; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*
D001843:Bone Cements; D002130:Calcium Phosphates; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; C020243:calcium phosphate