A new tool to detect high viscous exopolymers from microalgae.
Microalgae are microorganisms often surrounded by a slime layer made of secreted polymeric substances sometimes including polysaccharides. These polysaccharides, weakly described in the literature, can constitute value-added molecules in several industrial areas. The aim of this article is to show that a new tool, the BioFilm Ring Test®, can be used to detect viscous microalgal exopolymers. Two red microalgal strains (Rhodella violacea and Porphyridium purpureum), one cyanobacterium (Arthrospira platensis) and their excreted polymeric fractions were studied. R. violacea and P. purpureum induced a positive response with the BioFilm Ring Test® contrary to A. platensis. Finally, the understanding of the fractions viscosity involvement in the BRT response was performed by a rheological study.
Badel
S
S
Laboratoire de Génie Chimique et Biochimique, Clermont Université, Université Blaise Pascal, Polytech' Clermont Ferrand, 24 avenue des Landais, Aubière Cedex, France.
Callet
F
F
Laroche
C
C
Gardarin
C
C
Petit
E
E
El Alaoui
H
H
Bernardi
T
T
Michaud
P
P
D018441:Biofilms; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D000458:Cyanobacteria / Q000737:chemistry / Q000254:growth & development; D058086:Microalgae / Q000737:chemistry* / Q000254:growth & development; D011134:Polysaccharides / Q000032:analysis*; D044665:Porphyridium / Q000737:chemistry / Q000254:growth & development; D000461:Rhodophyta / Q000737:chemistry / Q000254:growth & development; D014783:Viscosity
D003470:Culture Media; D011134:Polysaccharides