title: Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy.
abstract: Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.
authors.affiliation: University of Munster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, Munster, 48149 Germany. bkemper@unimuenster.de
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
authors.forename: Andreas
authors.initials: A
authors.lastname: Bauwens
authors.forename: Angelika
authors.initials: A
authors.lastname: Vollmer
authors.forename: Steffi
authors.initials: S
authors.lastname: Ketelhut
authors.forename: Patrik
authors.initials: P
authors.lastname: Langehanenberg
authors.forename: Johannes
authors.initials: J
authors.lastname: Müthing
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
authors.forename: Gert
authors.initials: G
authors.lastname: von Bally
mesh_terms: D001921:Brain / Q000166:cytology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006696:Holography / Q000379:methods*; D006801:Humans; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D055806:Microvessels / Q000166:cytology; D013997:Time Factors