Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy.
Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.
Kemper
Björn
B
University of Munster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, Munster, 48149 Germany. bkemper@unimuenster.de
Bauwens
Andreas
A
Vollmer
Angelika
A
Ketelhut
Steffi
S
Langehanenberg
Patrik
P
Müthing
Johannes
J
Karch
Helge
H
von Bally
Gert
G
D001921:Brain / Q000166:cytology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006696:Holography / Q000379:methods*; D006801:Humans; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D055806:Microvessels / Q000166:cytology; D013997:Time Factors