title: Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice--roles of endothelial progenitor cells and nitric oxide.
abstract: OBJECTIVE
  Circulating endothelial progenitor cells (EPCs) play a significant role in postnatal neovascularization. Patients with diabetes have attenuated EPC functions and impaired angiogenic response after tissue ischemia. We investigated whether moderate red wine consumption can enhance blood flow recovery in response to tissue ischemia by enhancement of EPC functions in diabetic mice.
  
  
  RESULTS
  Starting at 4 weeks after diabetes onset, red wine (4 ml/kg/day) or ethanol were administered to streptozotocin (STZ)-induced (type 1) diabetic mice and KKAy-Ta (type 2) mice. Unilateral hind limb ischemia surgery was conducted after 2 weeks of red wine or ethanol ingestion. Type 1 and type 2 diabetic mice given red wine, but not ethanol, had significantly increased collateral flow about 30% and augmented capillary density in ischemic tissues. These beneficial effects were markedly abolished by an eNOS inhibitor (L-NAME). Flow cytometry analysis showed impaired EPC-like cells (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice, but augmented mobilization in red wine group (baseline vs. 2 days after operation: 0.88±0.06% vs. 1.73±0.29%, p=0.010). C-kit positive bone marrow cells isolated from diabetic mice given red wine had enhanced adhesion and migration compared to mice given vehicle. By in-vitro studies, incubation with red wine in high-glucose medium significantly reduced H2O2 production, and improved high glucose-suppressed EPC functions by nitric oxide-related mechanisms.
  
  
  CONCLUSIONS
  Our findings demonstrate that red wine consumption enhances blood flow recovery after tissue ischemia in diabetic mice. These effects may partly derive from enhanced EPC functions by upregulation of eNOS activity.
authors.affiliation: Division of Cardiology, Department of Medical Research and Education, Taipei Veterans General Hospital, Institute of Clinical Medicine, Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Po-Hsun
authors.initials: PH
authors.lastname: Huang
authors.forename: Hsiao-Ya
authors.initials: HY
authors.lastname: Tsai
authors.forename: Chao-Hung
authors.initials: CH
authors.lastname: Wang
authors.forename: Yung-Hsiang
authors.initials: YH
authors.lastname: Chen
authors.forename: Jia-Shiong
authors.initials: JS
authors.lastname: Chen
authors.forename: Feng-Yen
authors.initials: FY
authors.lastname: Lin
authors.forename: Chih-Pei
authors.initials: CP
authors.lastname: Lin
authors.forename: Tao-Cheng
authors.initials: TC
authors.lastname: Wu
authors.forename: Masataka
authors.initials: M
authors.lastname: Sata
authors.forename: Jaw-Wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Shing-Jong
authors.initials: SJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002196:Capillaries / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000628:therapy; D042783:Endothelial Cells / Q000166:cytology*; D000431:Ethanol / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods; D007511:Ischemia / Q000378:metabolism*; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018919:Neovascularization, Physiologic; D009569:Nitric Oxide / Q000378:metabolism*; D010766:Phosphorylation; D013234:Stem Cells / Q000166:cytology*; D013311:Streptozocin / Q000494:pharmacology; D014920:Wine
chemical_list: D009569:Nitric Oxide; D000431:Ethanol; D013311:Streptozocin; D019331:NG-Nitroarginine Methyl Ester