Survivin is upregulated in myeloma cell lines cocultured with mesenchymal stem cells.
In this study we investigated the influence of the mesenchymal stem cells on the survival of U266 and H929 myeloma cell lines. We found that both soluble factors and direct cell contact inhibited daunorubicin-induced apoptosis of U266 and H929 cells. The mRNA and protein levels of survivin were upregulated in U266 and H929 cells cocultured with mesenchymal stem cells, whereas not increased in that separated by TW insert. Antisense oligonucleotides targeting survivin downregulated the expression of survivin and increased the apoptosis of U266 and H929 cells. The results indicated that MSCs had anti-apoptotic effect on U266 and H929 cells that was mediated by survivin.
Wang
Xiaofang
X
Department of Hematology, Cancer Hospital of Tianjin, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Ti-Yuan-Bei, Huan-Hu-Xi-Road, Tianjin 300060, PR China. xiaofangwang2005@163.com
Zhang
Zhiqing
Z
Yao
Cheng
C
D017209:Apoptosis; D002450:Cell Communication; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D009101:Multiple Myeloma / Q000378:metabolism / Q000473:pathology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D000077022:Survivin; D015854:Up-Regulation
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D000077022:Survivin