Targeted nanoparticles enhanced flow electroporation of antisense oligonucleotides in leukemia cells.
Liposome nanoparticles (LNs) with a targeting ligand were used in a semi-continuous flow electroporation (SFE) device to enhance in vitro delivery of exogenous oligonucleotides (ODN). Nanoparticles comprising transferrin-targeted lipoplex encapsulating ODN G3139 were mixed with K562 cells (a chronic myeloid leukemia cell line) and incubated for half an hour to accomplish nanoparticle binding. The mixture was then flowed through a SFE channel where electric pulses were given. Better ODN delivery efficiency was achieved with an increase of ∼24% to the case in combination of non-targeted LNs and SFE, and ∼60% to the case using targeted LNs alone, respectively. The MTS assay results confirmed cell viability greater than 75%.
Wang
Shengnian
S
Chemical Engineering Program, Louisiana Tech University, Ruston, Louisiana 71272, United States.
Zhang
Xulang
X
Yu
Bo
B
Lee
Robert J
RJ
Lee
L James
LJ
D016373:DNA, Antisense / Q000008:administration & dosage / Q000235:genetics* / Q000493:pharmacokinetics*; D018274:Electroporation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D018390:Gene Targeting / Q000295:instrumentation*; D006801:Humans; D020014:K562 Cells; D014162:Transfection / Q000295:instrumentation*
D016373:DNA, Antisense