title: Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses".
abstract: We present a detailed analysis of the genome architecture, structural proteome and infection-related properties of three Pseudomonas phages, designated LUZ7, LIT1 and PEV2. These podoviruses encapsulate 72.5 to 74.9 kb genomes and lyse their host after 25 min aerobic infection. PEV2 can successfully infect under anaerobic conditions, but its latent period is tripled, the lysis proceeds far slower and the burst size decreases significantly. While the overall genome structure of these phages resembles the well-studied coliphage N4, these Pseudomonas phages encode a cluster of tail genes which displays significant similarity to a Pseudomonasaeruginosa (cryptic) prophage region. Using ESI-MS/MS, these tail proteins were shown to be part of the phage particle, as well as ten other proteins including a giant 370 kDa virion RNA polymerase. These phages are the first described representatives of a novel kind of obligatory lytic P. aeruginosa-infecting phages, belonging to the widespread "N4-like viruses" genus.
authors.affiliation: Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg, Leuven, B-3001, Belgium.
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.forename: Andrew
authors.initials: A
authors.lastname: Brabban
authors.forename: Larissa
authors.initials: L
authors.lastname: Rogge
authors.forename: Matthew Spooner
authors.initials: MS
authors.lastname: Lewis
authors.forename: Derek
authors.initials: D
authors.lastname: Pickard
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.forename: Andrew
authors.initials: A
authors.lastname: Kropinski
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Kutter
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
mesh_terms: D000818:Animals; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000821:virology; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015678:Viral Structural Proteins