[Expression of endothelial protein C receptor in tumor cell lines and It's significance].
OBJECTIVE
To detect the role of activated protein C(APC) on proliferation of endothelial cell and investigate the expression of endothelial protein C receptor( EPCR) in variety of tumor cell lines.


METHODS
The effect of APC on endotheliocyte proliferation was determined by MTT colorimetry. IL-6 and IL-8 in supernatant were measured by ELISA. Expression of EPCR were measured by semi-quantitative RT-PCR.


RESULTS
APC can increase the proliferation of EC significantly. EPCR gene was found in 91.7% of solid tumors and 66.7% of hematopoietic malignancies.


CONCLUSIONS
APC can stimulate the proliferation of endothelial cell. High expression of EPCR in tumor cell lines provides a potential biological marker for malignancies.
Heng
Wei
W
Department of Respiratory, First Affliated Hospital, Suzhou University, Jiangsu Institute of Hematology, Suzhou 215006, China. hwei65@sohu.com
Huang
Jian-an
JA
Shen
Wen-hong
WH
Dai
Lan
L
Bai
Xia
X
Wang
Zhao-yue
ZY
D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D000074283:Endothelial Protein C Receptor; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D011486:Protein C / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
D015703:Antigens, CD; D000074283:Endothelial Protein C Receptor; D015850:Interleukin-6; D016209:Interleukin-8; C478370:PROCR protein, human; D011486:Protein C; D011956:Receptors, Cell Surface