Seasonal fluctuations in leaf phenolic composition under UV manipulations reflect contrasting strategies of alder and birch trees.
Seasonal variation in leaf phenolic composition may be important for acclimation of plants to seasonal changes in their biotic and abiotic environment. For a realistic assessment of how plants respond to solar UV-B (280-315 nm) and UV-A (315-400 nm) radiation, seasonal variation in both environment and plant responses needs to be taken into account. This also has implications for studies concerning stratospheric ozone depletion and resulting increased UV-B radiation, as other environmental variables and/or plant phenology could interact with UV radiation. To elucidate this, we established a field experiment using plastic films attenuating different parts of the solar UV spectrum. The concentration of individual phenolic compounds was measured during one growing season in leaves of grey alder (Alnus incana) and white birch (Betula pubescens) trees. Our results showed changes in concentration of, e.g. hydrolyzable tannins in birch that suggest an effect of UV-A alone and e.g. chlorogenic acids in alder indicate a quadratic effect of UV-B irradiance and both linear and quadratic effect for UV-A in second-degree polynomial fits. Further, there was interaction between treatment and sampling time for some individual metabolites; hence, the UV response varied during the season. In addition to the UV effects, three temporal patterns emerged in the concentrations of particular groups of phenolics. Possible implications for both sampling methods and timing are discussed. Moreover, our results highlight differences in responses of the two tree species, which are taken to indicate differences in their ecological niche differentiation.
Kotilainen
Titta
T
Department of Biological and Environmental Sciences, P.O. Box 35, FIN-40014, University of Jyväskylä, Jyväskylä, Finland. titta.kotilainen@helsinki.fi
Tegelberg
Riitta
R
Julkunen-Tiitto
Riitta
R
Lindfors
Anders
A
O'Hara
Robert B
RB
Aphalo
Pedro J
PJ
D029661:Alnus / Q000737:chemistry / Q000528:radiation effects*; D029662:Betula / Q000737:chemistry / Q000528:radiation effects*; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry* / Q000528:radiation effects; D012621:Seasons; D014197:Trees / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
D010636:Phenols