Difference of fibroblast growth factor receptor 1 expression among CA1-3 regions of the gerbil hippocampus after transient cerebral ischemia.
Fibroblast growth factors are important regulators of neuronal development. In this study, we observed fibroblast growth factor receptor 1 (FGFR1) immunoreactivity and its protein levels in the hippocampus proper (CA1-3 regions) of the gerbil at various time points after ischemia/reperfusion. In the sham-operated group, FGFR1 immunoreaction was not detected in the hippocampus proper. FGFR1 immunoreaction was first detected in non-pyramidal neurons in the CA1-3 region at 12h and 1day after ischemia/reperfusion. From 2days after ischemia/reperfusion, FGFR1 immunoreaction was found in astrocytes, not in microglial cells, in the CA1 region: FGFR1 immunoreactivity and the number of astrocytes were significantly increased at 5days post-ischemia. Western blot analysis revealed that FGFR1 protein levels were also increased from 1day after ischemia/reperfusion. These results indicate that increase of FGFR1 in astrocytes of the ischemic CA1 region may be associated with gliosis followed by delayed neuronal death.
Yoo
Ki-Yeon
KY
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea.
Hwang
In Koo
IK
Lee
Choong Hyun
CH
Choi
Jung Hoon
JH
Kwon
Seung-Hae
SH
Kang
Il-Jun
IJ
You
Sang Guan
SG
Kim
Young-Myeong
YM
Won
Moo-Ho
MH
D000818:Animals; D001253:Astrocytes / Q000473:pathology; D015153:Blotting, Western; D056547:CA1 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D056651:CA2 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D056654:CA3 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death; D003627:Data Interpretation, Statistical; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000473:pathology; D010320:Parvalbumins / Q000378:metabolism; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics*
D005904:Glial Fibrillary Acidic Protein; D010320:Parvalbumins; D051496:Receptor, Fibroblast Growth Factor, Type 1