Polypyrrole nanowire modified with Gly-Gly-His tripeptide for electrochemical detection of copper ion.
We developed a novel biosensor comprising a transducer of carboxyl end-capped overoxidized polypyrrole nanowire electrode and a probe of tripeptide (Gly-Gly-His) selectively cognitive of Cu2+. The developed sensor was demonstrated to specifically detect Cu2+ in the nanomolar range. The diameter of the polypyrrole nanowires, prepared by one step template-free polymerization, was approximately 60-90 nm. For functionalization of the electrode, the carboxyl group was introduced by the addition of pyrrole-α-carboxylic acid which was covalently coupled with the amine group of the tripeptide. The structural features of the peptide functionalized nanowire electrode were confirmed by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) spectroscopy. Using cyclic and square wave voltammetry, the nanowire biosensor proved to be highly sensitive to Cu2+ in the range of 20-300 nM.
Lin
Meng
M
Department of Chemical Engineering. Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Cho
Misuk
M
Choe
Woo-Seok
WS
Yoo
Ji-Beom
JB
Lee
Youngkwan
Y
D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D003300:Copper / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007477:Ions; D036103:Nanotechnology / Q000295:instrumentation*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D007477:Ions; D009842:Oligopeptides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; C037026:diglycyl-histidine; D003300:Copper