title: S-resistin inhibits adipocyte differentiation and increases TNFalpha expression and secretion in 3T3-L1 cells.
abstract: S-resistin is a non-secretable resistin spliced variant described in white adipose tissue from Wistar rats. Since resistin has been implicated in adipogenesis regulation, here we have investigated the possible role of this new isoform in this process. For that, we have studied the adipocyte development in 3T3-L1 pre-adipocyte cell line stably expressing s-resistin and resistin. Both isoforms are able to restrain 3T3-L1 pre-adipocyte differentiation though affecting differently the expression pattern of pro-adipogenic transcription factors such CCAAT/enhancer binding proteins alpha and beta (C/EBPalpha and C/EBPbeta) and peroxisome proliferator-activated receptor gamma (PPARgamma), as well of proteins implicated in lipid metabolism such perilipin, fatty acid synthase (FAS), adipocyte lipid binding protein (ALBP/aP2) and carnitine palmitoyltransferase1 (CPT1). Likewise, both resistin isoforms impair insulin-stimulated glucose transport by decreasing glucose transport 4 (GLUT4) expression but to a different degree. In addition, s-resistin expressing 3T3-L1 cells display other remarkable differences. Thus, in these cells, endogenous resistin expression falls down while tumor necrosis factor alpha (TNFalpha) and interleukine 6 (IL-6) productions are increased along differentiation. These findings indicate that s-resistin isoform also impairs adipocyte differentiation affecting the expression pattern of key pro-adipogenic transcription factors and insulin sensitivity. Additionally, s-resistin may play a role in inflammatory processes.
authors.affiliation: Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Toledo, Spain.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Fernández
authors.forename: Araceli
authors.initials: A
authors.lastname: del Arco
authors.forename: Nilda
authors.initials: N
authors.lastname: Gallardo
authors.forename: Lidia
authors.initials: L
authors.lastname: Aguado
authors.forename: María
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Manuel
authors.initials: M
authors.lastname: Ros
authors.forename: Jose M
authors.initials: JM
authors.lastname: Carrascosa
authors.forename: Antonio
authors.initials: A
authors.lastname: Andrés
authors.forename: Carmen
authors.initials: C
authors.lastname: Arribas
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D020869:Gene Expression Profiling; D005947:Glucose / Q000493:pharmacokinetics; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D052243:Resistin / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D007004:Hypoglycemic Agents; D007328:Insulin; D015850:Interleukin-6; D047495:PPAR gamma; D020033:Protein Isoforms; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; D005947:Glucose