title: Immobilization of iron oxide magnetic nanoparticles for enhancement of vessel wall magnetic resonance imaging--an ex vivo feasibility study.
abstract: Emerging data supports a role for negative wall remodeling in the failure of vascular interventions such as vein grafts, yet clinicians/researchers currently lack the ability to temporally/efficiently investigate adventitial surface topography/total vascular wall anatomy in vivo. We established a strategy of immobilizing commercially available iron oxide magnetic nanoparticles (Fe-NPs) onto the surface of human vein conduits to facilitate high-throughput total vascular wall demarcation with magnetic resonance (MR). Binding of activated Fe-NPs to amine groups on the surface of the veins induced a thin layer of negative contrast that differentiated the adventitia from surrounding saline signal in all MR images, enabling delineation of total wall anatomy; this was not possible in simultaneously imaged unlabeled control veins. Under the conditions of this ex vivo experiment, stable covalent binding of Fe-NPs can be achieved (dose-dependent) on human vein surface for MR detection, suggesting a potential strategy for enhancing the ability of MRI to investigate total wall adaptation and remodeling in vein graft failure.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard-MIT Division of Heath Sciences &Technology, 65 Landsdowne Street, Cambridge, MA 02139, USA.
authors.forename: Binh Thai
authors.initials: BT
authors.lastname: Nguyen
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Vemula
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Mitsouras
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Tao
authors.forename: Christina
authors.initials: C
authors.lastname: Campagna
authors.forename: Robert V
authors.initials: RV
authors.lastname: Mulkern
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Rybicki
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
authors.forename: C Keith
authors.initials: CK
authors.lastname: Ozaki
mesh_terms: D001665:Binding Sites; D001808:Blood Vessels / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide