title: Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein.
abstract: In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L. japonicus and Mesorhizobium loti. When TARF was silenced by virus-induced gene silencing (VIGS) method, TMV RNA accumulation as well as the number of foci formed by GFP-tagged TMV increased drastically. Transient overexpression of TARF reduced the accumulation of TMV. Moreover, TARF transcription was rapidly upregulated by the inoculation of TMV in tobacco plants. These results indicated that TARF is a RING finger protein that inhibits the accumulation of TMV via the interaction of TMV RdRp.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ayyamaji@mail.ecc.u-tokyo.ac.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Koji
authors.initials: K
authors.lastname: Hamada
authors.forename: Toshio
authors.initials: T
authors.lastname: Yoshinuma
authors.forename: Keitaro
authors.initials: K
authors.lastname: Sakurai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yoshii
authors.forename: Takumi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Hibi
mesh_terms: D054884:Host-Pathogen Interactions; D000070116:Lotus / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000276:immunology* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development / Q000276:immunology*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase