title: Participation of the Candida albicans surface antigen in adhesion, the first phase of biofilm development.
abstract: The lack of work dealing with possible ways of reducing biofilm production via inhibiting Candida albicans adherence in the first stage of biofilm formation was a motivation for this study. The study was focused on two questions: (1) can a decrease in adherence affect the quantity of mature biofilm? and (2) can blocking the surface C. albicans complement receptor 3-related protein (CR3-RP) with polyclonal anti-C3-RP antibody or monoclonal antibody OKM1 significantly contribute to a reduction in adherence during biofilm formation? The presence and quantity the CR3-RP expressed in the biofilm was confirmed by immunofluorescence, immunocytometry and enzyme-linked immunosorbent assay. To determine the changes in adherence of C. albicans CCY 29-3-162 and C. albicans catheter isolate, 30-, 60-, 90- and 120-min time points were selected and viability was determined by XTT assay. The strains were preincubated with both antibodies to block CR3-RP, which proved to be effective at reducing adhesion and the formation of a mature biofilm (64.1-74.6%). The duration of adhesion, between 30 and 120 min, seems to have a significant effect on the mature biofilm. The blocking of CR3-PR by antibodies before adherence affected the fitness of biofilm, which was not able to revitalize in the later stages.
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. bujdakova@fns.uniba.sk
authors.forename: Helena
authors.initials: H
authors.lastname: Bujdáková
authors.forename: Ema
authors.initials: E
authors.lastname: Paulovicová
authors.forename: Lucia
authors.initials: L
authors.lastname: Paulovicová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Simová
mesh_terms: D000908:Antibodies, Fungal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D000908:Antibodies, Fungal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D013778:Tetrazolium Salts; C097687:alphaINT1 protein, Candida albicans; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide