Amplification of IGH/CCND1 fusion gene in a primary plasma cell leukemia case.
The IGH/CCND1 fusion gene has been reported in many hematologic tumors such as mantle cell lymphoma, chronic lymphocytic leukemia, prolymphocytic leukemia, multiple myeloma, and plasma cell leukemia. We report a case of plasma cell leukemia showing five IGH/CCND1 fusion signals by interphase fluorescence in situ hybridization (FISH). Conventional cytogenetic analysis and multicolor spectral karyotyping showed a complex karyotype that did not include t(11;14). Metaphase FISH studies revealed three IGH/CCND1 fusion signals, two of which were amplified signals. These findings indicate that IGH/CCND1 fusion gene was amplified and interspersed in several chromosomes. The patient was treated with intensive chemotherapy. However, the clinical course was very aggressive. The amplification of the IGH/CCND1 fusion gene may contribute to the aggressive course of the disease. To our knowledge, this is the first case showing amplification of the IGH/CCND1 gene in plasma cell neoplasms.
Ishigaki
Tomohiro
T
Department of Clinical Laboratory, Tokyo Medical and Dental University Hospital, 1-5-45 Yushima Bunkyo-ku, Tokyo, Japan. ishigaki.mlab@tmd.ac.jp
Sasaki
Kouji
K
Watanabe
Ken
K
Nakamura
Norihiko
N
Toyota
Shigeo
S
Kobayashi
Hirofumi
H
Tohda
Shuji
S
D019938:Cyclin D1 / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007952:Leukemia, Plasma Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D032681:Spectral Karyotyping
C530104:CCND1 protein, human; D007143:Immunoglobulin Heavy Chains; D019938:Cyclin D1