title: [Quantitative expression of voltage-dependent calcium channels in prostate smooth muscle cells of rats with estradiol-induced chronic nonbacterial prostatitis].
abstract: OBJECTIVE
  To construct a rat model of chronic nonbacterial prostatitis (CP) and investigate the difference in the quantitative expression of voltage-dependent calcium channels of prostate smooth muscle cells (PSMCs) between the models and controls.
  
  
  METHODS
  We established a CP rat model by estrogen induction, cultured and purified the PSMCs in vitro, and extracted total RNA by Trizol. Then we measured the mRNA expression of the cal subunit in the calcium channel subtypes by reverse transcription and SYBR Green I real time RT-PCR, and compared it with that of the controls.
  
  
  RESULTS
  The expressions of the L-, T- and P/Q-type calcium channels were found in both the CP and control groups, and that of the CaV1.2 L-type calcium channel was significantly increased in the former as compared with the latter (0.048 +/- 0.024 versus 0.031 +/- 0.015, t = 2.846, P = 0.007), but there were no statistically significant differences in the T- and P/Q-type calcium channels between the two groups.
  
  
  CONCLUSIONS
  The number of CaV1.2 L-type calcium channels of PSMCs and calcium influx were increased in CP patients, which may be involved in the mechanism of CP.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. zlcommandos2@yahoo.com.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Chao-Zhao
authors.initials: CZ
authors.lastname: Liang
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Zhang
authors.forename: Zong-Yao
authors.initials: ZY
authors.lastname: Hao
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Song
authors.initials: S
authors.lastname: Fan
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020907:Calcium Channels, Q-Type / Q000378:metabolism*; D020747:Calcium Channels, T-Type / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism; D011472:Prostatitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020746:Calcium Channels, L-Type; D020907:Calcium Channels, Q-Type; D020747:Calcium Channels, T-Type; C419385:L-type calcium channel alpha(1C); D012333:RNA, Messenger; D004958:Estradiol