[A real-time polymerase chain reaction-based test system for quantitation of Gumboro disease virus].
A real-time polymerase chain reaction-based test system for quantitation of infectious bursal disease (Gumboro disease) virus was developed. The reaction parameters were analyzed, which affected the linear relationship of a C1 depentanizer to the quantity of cDNA. The use of specific primers for reverse transcription was shown tohave some advantage over that of random hexanucleotides.
Pogoda
A A
AA
Potekhina
M A
MA
D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000821:virology; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D036022:Capsid Proteins / Q000235:genetics; D002645:Chickens; D017931:DNA Primers; D007243:Infectious bursal disease virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D036022:Capsid Proteins; D017931:DNA Primers