title: Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly.
abstract: In recent years, the opportunistic pathogen Pseudomonas aeruginosa has emerged as a major source of hospital-acquired infections. Effective treatment has proven increasingly difficult due to the spread of multidrug resistant strains and thus requires a deeper understanding of the biochemical mechanisms of pathogenicity. The central carbohydrate of the P. aeruginosa PAO1 (O5) B-band O-antigen, ManNAc(3NAc)A, has been shown to be critical for virulence and is produced in a stepwise manner by five enzymes in the Wbp pathway (WbpA, WbpB, WbpE, WbpD, and WbpI). Herein, we present the crystal structure of the aminotransferase WbpE from P. aeruginosa PAO1 in complex with the cofactor pyridoxal 5'-phosphate (PLP) and product UDP-GlcNAc(3NH(2))A as the external aldimine at 1.9 A resolution. We also report the structures of WbpE in complex with PMP alone as well as the PLP internal aldimine and show that the dimeric structure of WbpE observed in the crystal structure is confirmed by analytical ultracentrifugation. Analysis of these structures reveals that the active site of the enzyme is composed of residues from both subunits. In particular, we show that a key residue (Arg229), which has previously been implicated in direct interactions with the alpha-carboxylate moiety of alpha-ketoglutarate, is also uniquely positioned to bestow specificity for the 6''-carboxyl group of GlcNAc(3NH(2))A through a salt bridge. This finding is intriguing because while an analogous basic residue is present in WbpE homologues that do not process 6''-carboxyl-modified saccharides, recent structural studies reveal that this side chain is retracted to accommodate a neutral C6'' atom. This work represents the first structural analysis of a nucleotide sugar aminotransferase with a bound product modified at the C2'', C3'', and C6'' positions and provides insight into a novel target for treatment of P. aeruginosa infection.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: Angelyn
authors.initials: A
authors.lastname: Larkin
authors.forename: Nelson B
authors.initials: NB
authors.lastname: Olivier
authors.forename: Barbara
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D000409:Alanine / Q000235:genetics; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D009154:Mutation; D019884:Nitrogenous Group Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019081:O Antigens / Q000378:metabolism; D011485:Protein Binding; D011552:Pseudomonas Infections / Q000201:enzymology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D011732:Pyridoxal Phosphate / Q000737:chemistry / Q000378:metabolism*; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D012545:Schiff Bases / Q000737:chemistry / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D019081:O Antigens; D012545:Schiff Bases; C552373:UDP-2-acetamido-3-amino-2,3-dideoxyglucuronic acid; D014535:Uridine Diphosphate Glucuronic Acid; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D019884:Nitrogenous Group Transferases; D000409:Alanine; C010627:pyridoxamine phosphate