title: Comparative study on responses of cattle and water buffalo (Bubalus bubalis) to experimental inoculation of Brucella abortus biovar 1 by the intraconjunctival route--a preliminary report.
abstract: The preliminary study was conducted to assess the virulence of a strain of Brucella abortus (1969D) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. Seven of each cattle and water buffalo (Bubalus bubalis) calves aged 3-6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10(7) to 1.7 × 10(10) colony forming units of B. abortus. Animals were monitored over an 8-week period for clinical manifestations and serological and hematological evidence of infection. At slaughter, eight lymph nodes from each animal were sampled for bacteriological and histopathological assessments. Lymph nodes from three water buffalo (43%) and five cattle (71%) yielded B. abortus (P=0.048). Parotid/prescapular lymph nodes were most sensitive in detecting B. abortus. Our data suggest that B. abortus strain 1969D may be used as challenge strain, and water buffalo appeared to have a lower susceptibility to B. abortus infection than cattle.
authors.affiliation: School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago. Abiodun.Adesiyun@sta.uwi.edu
authors.forename: Abiodun A
authors.initials: AA
authors.lastname: Adesiyun
authors.forename: Geoff T
authors.initials: GT
authors.lastname: Fosgate
authors.forename: Anil
authors.initials: A
authors.lastname: Persad
authors.forename: Mervyn
authors.initials: M
authors.lastname: Campbell
authors.forename: Ravi
authors.initials: R
authors.lastname: Seebaransingh
authors.forename: Alva
authors.initials: A
authors.lastname: Stewart-Johnson
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000472:pathogenicity*; D002007:Brucellosis, Bovine / Q000473:pathology*; D002020:Buffaloes; D002417:Cattle; D015169:Colony Count, Microbial / Q000662:veterinary; D003228:Conjunctiva / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D012698:Serologic Tests / Q000662:veterinary; D013045:Species Specificity; D018709:Statistics, Nonparametric; D014774:Virulence