[Inhibition of panaxynol on experimental delayed type hypersensitivity].
OBJECTIVE
To evaluate the effect of panaxynol (PAN) on delayed type hypersensitivity and possible mechanism.


METHODS
Allergic contact dermatitis (ACD) was induced by DNCB as a delayed type hypersensitivity (DTH) model to observe effect of PAN on auricle inflammation including pathological injury. Proliferation of T lymphocytes was induced by ConA and measured by MTf method. IFN-gamma secretion of splenocyte induced by ConA was detected by ELISA.


RESULTS
The swelling degree of auricle and pathological injury in ACD mice was reduced significantly by treated with PAN in induction phase. Proliferation of T lymphocytes induced by ConA in vitro was inhibited significantly by PAN, By contrast, no detectable effect was observed in resting splenocyte. IFN-y induced by ConA in splenocytes was inhibited markedly by PAN from 10 micromol x L(-1) and from 6 h.


CONCLUSIONS
The results showed that DTH was inhibited by PAN mainly in induction phase and this effect may be related with the inhibition on T lymphocytes proliferation and secretion of IFN-gamma.
Hong
Min
M
School of Phamacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. hongmin72@hotmail.com
Zheng
Jie
J
Wang
Liang
L
Kuang
Ye
Y
Wen
Hongmei
H
Wang
Xinzhi
X
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D053280:Diynes / Q000494:pharmacology* / Q000627:therapeutic use; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology
D053280:Diynes; D005233:Fatty Alcohols; D003208:Concanavalin A; C018541:falcarinol; D007371:Interferon-gamma