title: Characterization of genes encoding virulence determinants and toxins in Staphylococcus aureus from bovine milk in Central Italy.
abstract: The aim of this study was to evaluate the genotypic characteristics of Staphylococcus aureus isolates (n=170) from bovine milk collected from seven dairy farms in Italy. On the basis of cultural and biochemical properties and by amplification of the 23S rRNA specific to S. aureus, all isolates were identified as S. aureus. To genotypically characterize S. aureus isolates, genes encoding virulence determinants (nuc, clfA, spa-IgG-binding, spa-X-region, fnbA and fnbB, cap5 and cap8) and staphylococcal enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, sej) were investigated using a PCR technique. The results showed that the isolates of S. aureus in each farm had the same genotypic characteristics, while the isolates genotipically differed between the different farms. The present study might help to understand the distribution of prevalent S. aureus strains in dairy farms.
authors.affiliation: Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine, University of Perugia, Perugia. patrizia.casagrandeproietti@unipg.it
authors.forename: Patrizia
authors.initials: P
authors.lastname: Casagrande Proietti
authors.forename: Giacomo
authors.initials: G
authors.lastname: Coppola
authors.forename: Annalisa
authors.initials: A
authors.lastname: Bietta
authors.forename: Maria
authors.initials: M
authors.lastname: Luisa Marenzoni
authors.forename: Doreene R
authors.initials: DR
authors.lastname: Hyatt
authors.forename: Mauro
authors.initials: M
authors.lastname: Coletti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Passamonti
mesh_terms: D000818:Animals; D002417:Cattle; D003030:Coagulase / Q000235:genetics; D017931:DNA Primers; D003612:Dairying / Q000592:standards; D005260:Female; D005784:Gene Amplification; D005798:Genes, Bacterial; D005838:Genotype; D005843:Geography; D007558:Italy; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000302:isolation & purification; D012756:Sheep; D013203:Staphylococcal Infections / Q000235:genetics / Q000662:veterinary; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics*
chemical_list: D003030:Coagulase; D017931:DNA Primers; D012338:RNA, Ribosomal, 23S; D013205:Staphylococcal Protein A