title: Single-laboratory validation of a method for the determination of phenols and phenates in disinfectant formulations by liquid chromatography with UV detection.
abstract: A single-laboratory validation study was conducted for an LC method using UV detection for the simultaneous determination of the active ingredients o-phenylphenol (OPP), p-tert-amylphenol (PTAP), and o-benzyl-p-chlorophenol (OBPCP) in disinfectant formulations. Samples were extracted, the extracts diluted with acidified methanol, and the active ingredients separated by LC with a gradient mobile phase and quantified by using UV detection at 285 nm. For each active ingredient, the RSD was < or = 3.7%, and the intermediate reproducibility was < or = 3.4%. The active ingredient content of the spiked samples analyzed in this study ranged from 0.075 to 10.1% for the individual phenol active ingredients. The average recovery ranges were 86.7-104.9, 82.8-115.6, and 91.6-114.7% for the active ingredients OPP, PTAP, and OBPCP, respectively, for the concentration range of 0.075-10.1%. This method, with a relatively short chromatographic run time (about 15 min), proved to be reliable and convenient for analyses of products or samples containing all or a combination of these phenol active ingredients.
authors.affiliation: Maryland Department of Agriculture, State Chemist Section, 50 Harry S. Truman Pkwy, Annapolis, MD 21401, USA.
authors.forename: Tommy
authors.initials: T
authors.lastname: Phillips
authors.forename: Adrian
authors.initials: A
authors.lastname: Burns
authors.forename: Kenneth
authors.initials: K
authors.lastname: McManus
authors.forename: Warren
authors.initials: W
authors.lastname: Bontoyan
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004003:Dichlorophen / Q000031:analogs & derivatives* / Q000032:analysis; D004202:Disinfectants / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D001713:Biphenyl Compounds; D004202:Disinfectants; D010636:Phenols; C019566:p-tert-amylphenol; C004660:clorophene; C004369:2-phenylphenol; D004003:Dichlorophen