title: Oral administration of Enterococcus faecalis EC-12 cell preparation improves villous atrophy after weaning through enhancement of growth factor expression in mice.
abstract: Lactic acid bacteria, either alive or dead, can improve villous atrophy caused by weaning in both piglets and mice. In this experiment, we tried to detect the molecules involved in this phenomenon with a real-time RT-PCR array approach. Weaning pups of mice were administered either a suspension of an Enterococcus faecalis EC-12 dried cell preparation (EC-12) or saline for 11 consecutive days after weaning. The jejunal and ileal villous heights were measured histologically, and the expression levels of 86 genes were analyzed for the jejunal and ileal epithelial cells and the lamina propria (LP). EC-12 induced significantly higher villous height in the jejunum and the ileum. Interleukin (IL)-6, fibroblast growth factor (FGF)-7, -10, and -22, and the platelet-derived growth factor (PDGF) beta in the jejunal and the ileal LP were the most enhanced genes by EC-12. The possible role of these molecules in the improvement of villous atrophy is discussed.
authors.affiliation: Laboratory of Animal Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. k_ushida@kpu.ac.jp
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ushida
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Takumi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Ryo
authors.initials: R
authors.lastname: Inoue
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D013293:Enterococcus faecalis; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014886:Weaning
chemical_list: D017931:DNA Primers; D015850:Interleukin-6; D013006:Growth Hormone