Expression and intracellular localization of duck enteritis virus pUL38 protein.
Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.
Xiang
Jun
J
Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan, Sichuan, China.
Ma
Guangpeng
G
Zhang
Shunchuan
S
Cheng
Anchun
A
Wang
Mingshu
M
Zhu
Dekang
D
Jia
Renyong
R
Luo
Qihui
Q
Chen
Zhengli
Z
Chen
Xiaoyue
X
D000818:Animals; D002460:Cell Line; D004372:Ducks; D004751:Enteritis / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D025922:Mardivirus / Q000235:genetics* / Q000378:metabolism; D008380:Marek Disease / Q000378:metabolism / Q000821:virology*; D008970:Molecular Weight; D011201:Poultry Diseases / Q000378:metabolism / Q000821:virology*; D021381:Protein Transport; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D015678:Viral Structural Proteins