Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil.
BACKGROUND
Dengue is associated with explosive urban epidemics and has become a major public health problem in many tropical developing countries, including Brazil. The laboratory diagnosis of dengue can be carried out using several approaches, however sensitive and specific assays useful to diagnose in the early stage of fever are desirable. The flavivirus non-structural protein NS1, a highly conserved and secreted glycoprotein, is a candidate protein for rapid diagnosis of dengue in endemic countries.


RESULTS
We aimed to evaluate the potential use of 3 commercial kits in a panel of 450 serum samples for early diagnosis of dengue in Brazil. The PanBio Early ELISA (PanBio Diagnostics) showed a sensitivity of 72.3% (159/220) and a specificity of 100%, while the sensitivity of the Platelia NS1 assay (Biorad Laboratories) was 83.6% (184/220). However, the highest sensitivity (89.6%; 197/220) was obtained by using the NS1 Ag Strip (Biorad Laboratories). A lower sensitivity was observed in DENV-3 cases by all 3 kits. Serum positive by virus isolation were more often positive than cases positive by RT-PCR by all three assays and a higher detection rate was observed during the first four days after the onset of the symptoms. The presence or absence of IgM showed no influence in the confirmation by the pan-E Early ELISA (P = 0,6159). However, a higher confirmation by both Platelia NS1 (Biorad) and Dengue NS1 Ag Strip (Biorad) in the absence of IgM was statistically significant (P<0,0001 and P = 0,0008, respectively). Only the Platelia NS1 test showed a higher sensitivity in confirming primary infections than secondary ones.


CONCLUSIONS
The results indicate that commercial kits of dengue NS1 antigen are useful for the laboratory diagnosis of acute primary and secondary dengue. It can be used in combination with the MAC-ELISA for case detection and as screening test to complement viral isolation.
Lima
Monique da Rocha Queiroz
Mda R
Flavivirus Laboratory, Oswaldo Cruz Institute/FIOCRUZ, Rio de Janeiro, Brazil.
Nogueira
Rita Maria Ribeiro
RM
Schatzmayr
Hermann Gonçalves
HG
dos Santos
Flavia Barreto
FB
D000914:Antibodies, Viral / Q000097:blood; D001938:Brazil; D003715:Dengue / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007075:Immunoglobulin M / Q000097:blood; D012367:RNA, Viral / Q000097:blood; D017361:Viral Nonstructural Proteins / Q000097:blood*; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; C100315:NS1 protein, Flavivirus; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins