Targeted metabolomics for biomarker discovery.
Metabolomics is a truly interdisciplinary field of science, which combines analytical chemistry, platform technology, mass spectrometry, and NMR spectroscopy with sophisticated data analysis. Applied to biomarker discovery, it includes aspects of pathobiochemistry, systems biology/medicine, and molecular diagnostics and requires bioinformatics and multivariate statistics. While successfully established in the screening of inborn errors in neonates, metabolomics is now widely used in the characterization and diagnostic research of an ever increasing number of diseases. In this Review we highlight important technical prerequisites as well as recent developments in metabolomics and metabolomics data analysis with special emphasis on their utility in biomarker identification and qualification, as well as targeted metabolomics by employing high-throughput mass spectrometry.
Griffiths
William J
WJ
Swansea University, School of Medicine, Institute of Mass Spectrometry, Singleton Park, Swansea SA2 8PP, UK.
Koal
Therese
T
Wang
Yuqin
Y
Kohl
Matthias
M
Enot
David P
DP
Deigner
Hans-Peter
HP
D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods* / Q000639:trends
D015415:Biomarkers; D008055:Lipids