SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro.
Childhood spinal muscular atrophy is caused by a reduced expression of the survival motor neuron (SMN) protein. SMN has been implicated in the axonal transport of beta-actin mRNA in both primary and transformed neuronal cell lines, and loss of this function could account, at least in part, for spinal muscular atrophy onset and pathological specificity. Here we have utilised a targeted screen to identify mRNA associated with SMN, Gemin2 and Gemin3 in the cytoplasm of a human neuroblastoma cell line, SHSY5Y. Importantly, we have provided the first direct evidence that beta-actin mRNA is present in SMN cytoplasmic complexes in SHSY5Y cells.
Todd
Adrian G
AG
Clinical Neurobiology, Institute of Biomedical and Clinical Science, Peninsula Medical School, St. Luke's Campus, Heavitree Road, Exeter EX1 2LU, UK.
Morse
Robert
R
Shaw
Debra J
DJ
McGinley
Samuel
S
Stebbings
Howard
H
Young
Philip J
PJ
D000199:Actins / Q000235:genetics*; D001369:Axons; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism*; D055541:DEAD Box Protein 20 / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D055533:Survival of Motor Neuron 1 Protein / Q000378:metabolism*
D000199:Actins; C108381:GEMIN2 protein, human; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D055533:Survival of Motor Neuron 1 Protein; D055541:DEAD Box Protein 20