title: A sandwiched biological fluorescent probe for the diagnosis of human ovarian tumor based on TiO2 nanoparticles.
abstract: In this paper, we report a novel biological fluorescent probe for the diagnosis of human ovarian tumor based on sandwiched TiO(2) nanoparticles. The fluorescence nanoparticles consist of a fluorescent molecule, tetramethyl rhodamine isothiocyanate (TRITC), sandwiched between titanium dioxide (TiO(2)) nanoparticles and nano-gold via reacting with each other. The antibodies HER2, labeled on the surface of the biofluorescence nanoparticles, have granted nanoparticles the privilege of aiming at peculiar tumor antigen. The specificity of antibody-nanoparticles interacting with cells was characterized by Laser Scanning Confocal Microscope. The results showed that these sandwiched nanoparticles were innocuous and stable, and the method offered potential advantages of sensitivity and simplicity due to high combing efficiency between nanoparticles and cells and provided an alternative method for the diagnosis of human ovarian tumor (HOT).
authors.affiliation: College of Life and Environment, Shanghai Normal University, Shanghai, China.
authors.forename: Peisi
authors.initials: P
authors.lastname: Zhu
authors.forename: Shasheng
authors.initials: S
authors.lastname: Huang
authors.forename: Mengyao
authors.initials: M
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Ding
authors.forename: Bing
authors.initials: B
authors.lastname: Peng
authors.forename: Lingmi
authors.initials: L
authors.lastname: Kong
authors.forename: Yang
authors.initials: Y
authors.lastname: Bo
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology; D014025:Titanium
chemical_list: D005456:Fluorescent Dyes; C009495:titanium dioxide; D014025:Titanium