title: Children with atopic histories exhibit impaired lipopolysaccharide-induced Toll-like receptor-4 signalling in peripheral monocytes.
abstract: BACKGROUND
  The hygiene hypothesis states that early exposure to bacterial products such as lipopolysaccharide (LPS) may be protective against the development of allergic diseases. Whether atopic disease affects the ability of immune cells to respond to LPS is unclear. Our laboratory has demonstrated previously that children express high levels of Toll-like receptor (TLR)-4 on CD4(+) cells in nasal mucosa.
  
  
  OBJECTIVE
  To determine if children with a history of allergic disease have impaired responses to LPS on circulating CD4(+) leucocytes.
  
  
  METHODS
  Peripheral blood mononuclear cells from children (aged 2-18) and adults with or without a history of atopic conditions were cultured with/without IL-4 or LPS for up to 24 h. Expression of surface TLR-4, CD14, CD4, CD3, as well as of intracellular phosphorylated (p42/p44) ERK and p38 mitogen-activated protein kinase (MAPK) were assessed by flow cytometry.
  
  
  RESULTS
  A history of atopy in children was associated with impaired LPS-induced TLR-4-dependent phosphorylation of (p42/44) ERK and p38 MAPK by CD4(+) monocytes. Decreased LPS signalling was reproduced by pre-incubation of control cells with recombinant IL-4. LPS stimulation also decreased TLR-4 expression on monocytes from children without atopic histories but not from atopic subjects. CD4(+) T lymphocytes showed limited LPS responsiveness, regardless of atopic status. In contrast with non-atopic children, TLR-4 expression on monocytes of children with atopic histories decreased as a function of age.
  
  
  CONCLUSIONS
  This study provides evidence for defective LPS recognition on circulating CD4(+) leucocytes of subjects with atopic histories compared with those from non-atopic children. CD4(+) TLR4(+) monocytes from children with atopic histories failed to phosphorylate MAPKs. Our results suggest that a history of atopic disease is associated with impaired TLR-4-mediated innate immune function compared with non-atopic children.
authors.affiliation: Meakins Christie Laboratories and Montreal Children's Hospital Research Institute, McGill University Health Center Research Institute, Montreal, QC, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Préfontaine
authors.forename: A-A
authors.initials: AA
authors.lastname: Banville-Langelier
authors.forename: P-O
authors.initials: PO
authors.lastname: Fiset
authors.forename: J
authors.initials: J
authors.lastname: Guay
authors.forename: J
authors.initials: J
authors.lastname: An
authors.forename: M
authors.initials: M
authors.lastname: Mazer
authors.forename: Q
authors.initials: Q
authors.lastname: Hamid
authors.forename: B D
authors.initials: BD
authors.lastname: Mazer
mesh_terms: D000293:Adolescent; D000328:Adult; D017252:CD3 Complex / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007113:Immunity, Innate / Q000187:drug effects*; D015847:Interleukin-4 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D010766:Phosphorylation; D011792:Quebec; D015398:Signal Transduction / Q000187:drug effects*; D051197:Toll-Like Receptor 4 / Q000819:agonists* / Q000378:metabolism; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; C508598:IL4 protein, human; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D015847:Interleukin-4; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases