title: The bovine lactophorin C-terminal fragment and PAS6/7 were both potent in the inhibition of human rotavirus replication in cultured epithelial cells and the prevention of experimental gastroenteritis.
abstract: Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-M(r) glycoprotein fraction (F1) from cow's milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 degrees C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Inagaki
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nagai
authors.forename: Tomio
authors.initials: T
authors.lastname: Yabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagaoka
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Minamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakagomi
authors.forename: Toyoko
authors.initials: T
authors.lastname: Nakagomi
authors.forename: Takusaburo
authors.initials: T
authors.lastname: Ebina
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kanamaru
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000821:virology; D005759:Gastroenteritis / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D051379:Mice; D008894:Milk Proteins / Q000737:chemistry* / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D012401:Rotavirus / Q000187:drug effects / Q000502:physiology*; D012400:Rotavirus Infections / Q000517:prevention & control; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D008562:Membrane Glycoproteins; D008894:Milk Proteins; C106215:PAS-6-7 glycoprotein, Bos taurus; D010446:Peptide Fragments; C061124:lactophorin protein, Bos taurus