title: Identification of a new HIV type 1 circulating BF intersubtype recombinant form (CRF47_BF) in Spain.
abstract: We report the identification of a new HIV-1 circulating recombinant form (CRF47_BF) derived from subtypes B and F. It was initially identified in protease-reverse transcriptase sequences from nine individuals from three separate regions of Spain who acquired HIV-1 infection via sexual contact. All nine sequences formed a strongly supported phylogenetic cluster, branching apart from all known CRFs, and in bootscan analyses were BF mosaics with two coincident breakpoints. Two epidemiologically unlinked viruses were sequenced in near full-length genomes, which exhibited identical mosaic structures, with 16 intersubtype breakpoints in a genome predominantly of subtype B. Subtype F segments of the new CRF failed to cluster with any of the near full-length genome subtype F sequences available in public databases. Recent dates of HIV-1 diagnoses and short genetic distances suggest a recent origin of this CRF. This is the tenth reported CRF_BF, the first apparently having originated outside of South America.
authors.affiliation: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Fernández-García
authors.forename: Lucía
authors.initials: L
authors.lastname: Pérez-Alvarez
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Cuevas
authors.forename: Elena
authors.initials: E
authors.lastname: Delgado
authors.forename: Mercedes
authors.initials: M
authors.lastname: Muñoz-Nieto
authors.forename: Gustavo
authors.initials: G
authors.lastname: Cilla
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Iribarren
authors.forename: Milagros
authors.initials: M
authors.lastname: Pinilla
authors.forename: Antonio
authors.initials: A
authors.lastname: Ocampo
authors.forename: Celia
authors.initials: C
authors.lastname: Miralles
authors.forename: Sonia
authors.initials: S
authors.lastname: Pérez-Castro
authors.forename: María
authors.initials: M
authors.lastname: González-Galeano
authors.forename: Rafael Ojea
authors.initials: RO
authors.lastname: de Castro
authors.forename: Matilde
authors.initials: M
authors.lastname: Trigo
authors.forename: Valentina
authors.initials: V
authors.lastname: García
authors.forename: Ana M
authors.initials: AM
authors.lastname: Sánchez
authors.forename: Michael M
authors.initials: MM
authors.lastname: Thomson
mesh_terms: D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain
chemical_list: C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1