[Effect of Matrine on cell cycle and human telomerase reverse transcriptase of human salivary adenoid cystic carcinoma].
OBJECTIVE
To investigate the effect of the traditional Chinese medicine Matrine on cell cycle and human telomerase reverse transcriptase (hTERT) of human ACC-M cell lines.


METHODS
Different concentrations of Matrine were used in the medium of ACC-M cells. Change of cell cycle were detected by flow cytometry after ACC-M cell were cultivated with different concentrations Matrine (0.25, 0.50, 0.75, 1.00 mg x mL(-1)). Expression of hTERT was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescene and flow cytometry quantitative analysis.


RESULTS
Matrine caused obviously the GdG1 phase block and inhibited proliferation of ACC-M cells. At same time, this effect was positive correlation to Matrine concentration and treat time. Matrine can inhibit the expression of hTERT mRNA and protein.


CONCLUSIONS
Matrine can obviously inhibit cell cycle and down-regulate expression of hTERT. Inhibition of cell cycle is possible correlation with down-regulation expression of hTERT.
Zhao
Jun-Fang
JF
Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Xie
Wei-Hong
WH
Li
Xin-Ming
XM
Chen
Wan-Tao
WT
Sun
Ming-Lei
ML
Fang
Zheng
Z
Sun
Qiang
Q
D000470:Alkaloids; D003528:Carcinoma, Adenoid Cystic; D002453:Cell Cycle; D006801:Humans; D011807:Quinolizines; D012333:RNA, Messenger; D019098:Telomerase; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D000093842:Matrines