Recombinant peroxidase production in species of lepidoptera frequently found in Argentina.
Horseradish peroxidase isozyme C (HRPC) is an important commercial biocatalyst. In this study, a screening of different lepidopteran species frequently found in Argentina to produce this protein was carried out. Two recombinant viruses were constructed: AcMNPV HRPC polyhedrin-minus (occ-), an intrahemocelical infective virus; and AcMNPV HRPC polyhedrin-plus (occ+), to achieve an oral infective baculovirus. Each lepidopteran species was infected either with AcMNPV HRPC occ- or AcMNPV HRPC occ+ and the harvesting days post-infection (dpi) were optimized. All species were susceptible to AcMNPV HRPC occ- infection, giving Spodoptera frugiperda the best yield: 41 μg per larva. Rachiplusia nu was highly susceptible to oral infection, reaching 22 μg per larva at 4 dpi. HRPC was purified by IMAC from S. frugiperda extracts with a yield of 86% and a purification factor of 29.
Romero
L
L
Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica (UBA), Junín 956, 1113, Ciudad Autónoma de Buenos Aires, Argentina.
Targovnik
A
A
Wolman
F
F
Fogar
M
M
Simonella
M
M
Cascone
O
O
Miranda
M
M
D000818:Animals; D001118:Argentina; D006735:Horseradish Peroxidase / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000201:enzymology / Q000821:virology; D007915:Lepidoptera / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics / Q000821:virology*
D019476:Insect Proteins; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase