title: Neuroprotective effects of consuming bovine colostrum after focal brain ischemia/reperfusion injury in rat model.
abstract: To investigate the neuroprotective effects of bovine colostrums (BC), we evaluate the ability of consuming BC after focal brain ischemia/reperfusion injury rat model to reduce serum cytokine levels and infarct volume, and improve neurological outcome. Sprague-Dawley rats were randomly divided into 4 groups; one sham operation and three experimental groups. In the experimental groups, MCA occlusion (2 h) and subsequent reperfusion (O/R) were induced with regional cerebral blood flow monitoring. One hour after MCAO/R and once daily during the experiment, the experimental group received BC while the other groups received 0.9% saline or low fat milk (LFM) orally. Seven days later, serum pro-inflammatory cytokine (IL-1beta, IL-6, and TNF-alpha) and anti-inflammatory cytokine (IL-10) levels were assessed. Also, the infarct volume was assessed by using a computerized image analysis system. Behavioral function was also assessed using a modified neurologic severity score and corner turn test during the experiment. Rats receiving BC after focal brain I/R showed a significant reduction (-26%/-22%) in infarct volume compared to LFM/saline rats, respectively (P < 0.05). Serum IL-1beta, IL-6, and TNF-alpha levels were decreased significantly in rats receiving BC compared to LFM/saline rats (P < 0.05). In behavioral tests, daily BC intake showed consistent and significant improvement of neurological deficits for 7 days after MCAO/R. BC ingestion after focal brain ischemia/reperfusion injury may prevent brain injury by reducing serum pro-inflammatory cytokine levels and brain infarct volume in a rat model.
authors.affiliation: Department of Emergency Medicine, College of Medicine, Kyung Hee University, Seoul 130-702, Korea.
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Choi
authors.forename: Young Gwan
authors.initials: YG
authors.lastname: Ko
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Oh Young
authors.initials: OY
authors.lastname: Kwon
authors.forename: Sun-Kyu
authors.initials: SK
authors.lastname: Kim
authors.forename: Chul
authors.initials: C
authors.lastname: Cheong
authors.forename: Ki-Hyo
authors.initials: KH
authors.lastname: Jang
authors.forename: Soon Ah
authors.initials: SA
authors.lastname: Kang