title: [Comparative studies in content of major active compositions in different parts of Cichorium glandulosum].
abstract: OBJECTIVE
  The four major active compositions, namely esculetin, lactucin, lactucopicrin and chlorogenic acid in seed, stem and root of the Cichorium glandulosum Boiss. et Huet that planted in Xinjiang have been quantified by HPLC.
  
  
  METHODS
  HPLC method was used, with Inertsil ODS-SP column (4.6 mm x 250 mm, 5 pm). The flow rate was 1.0 mL x min(-1). The column temperature was set at 32 degrees C. The mobile phase was methanol--0.2% formic acid, 0-40 min, methanol 30%--70% gradients. Injection volume was 5 microL. The detecting wavelength were 256, 350, 299 and 229 nm, respectively.
  
  
  RESULTS
  The percentage recoveries were 98.2%, 99.57%, 100.50%, and 99.46% for chlorogenic acid, esculetin, lactucin, and lactucopicrin, respectively. The correlation coefficients (r) were 1.000, 0.9989, 0.9998, 1.000 and RSD were 1.6%, 1.5%, 0.77%, 2.0% for chlorogenic acid, esculetin, lactucin, and lactucopicrin, respectively. The contents of the chlorogenic acid, esculetin, lactucin and lactucopicrin were 0.0048, 0.0043, 0.6789, 0.7520 mg x g(-1), respectively in the root, and 0.0710, 0.1890, 0.2396 and 0.0520 mg x g(-1) in the seeds of C. glandulosum, respectively.
  
  
  CONCLUSIONS
  This method was sensitive, rapid and simple, with good linearity, recovery and reproducibility.
authors.affiliation: College of Traditional Chinese Medical, Xinjiang Medical University, Urumqi 830054, China.
authors.forename: Tohtahon
authors.initials: T
authors.lastname: Zaynap
authors.forename: Jie
authors.initials: J
authors.lastname: Zhong
authors.forename: Xuelei
authors.initials: X
authors.lastname: Xin
authors.lastname: Hajiakber-Aisa
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D018514:Plant Structures / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D009930:Organic Chemicals