Differentiation of enzymatic from platelet hypercoagulability using the novel thrombelastography parameter delta (delta).
BACKGROUND
Thrombelastography (TEG) allows for rapid global assessment of coagulation function. Our previous work demonstrated that a hypercoagulable state identified by TEG's G value was associated with thromboembolic events in a cohort of critically ill surgical patients despite routine chemoprophylaxis. We hypothesized that a hypercoagulable state could be differentiated into enzymatic or platelet etiology through the use of thrombus velocity curves; specifically the time to maximum rate of thrombus generation (TMRTG) and the novel TEG parameter, delta. (Delta)


METHODS
We retrospectively studied 10 critically ill surgical patients receiving thromboprophylaxis for at least 72 h by TEG, using kaolin activated citrated samples. Thrombus velocity curves were plotted for each patient, and delta was calculated as the difference between the TEG parameters R and SP, corresponding to the time to maximum rate of thrombus generation (TMRTG), which reflects the enzymatic contribution to clot formation. The TEG parameter G, (G = 5000 x A/100-A) also was determined for each patient. As G is derived from amplitude (A), it reflects overall net clot strength. A hypercoagulable state was defined as delta < 0.6 min and/or G > 11 dynes/cm(2).


RESULTS
A hypercoagulable state was identified via delta in 6 patients (60%); all of whom remained hypercoagulable following heparinase addition, suggesting chemoprophylaxis was ineffective. Of six patients with a hypercoagulable G value, 50% had a normal delta suggesting the presence of platelet hypercoagulability. Delta closely correlated with TMRTG (r = 0.94). However, the varying contribution of platelets to hypercoagulability, was shown by a nonlinear, weak correlation of delta and TMRTG with G (r = 0.11 and r = 0.14, respectively).


CONCLUSIONS
Delta reflects changes in thrombin generation as measured by TMRTG, allowing for differentiation of enzymatic from platelet hypercoagulability. Future studies will be required to validate these findings.
Gonzalez
Eduardo
E
Department of Surgery, Denver Health Medical Center and University of Colorado School of Medicine, Denver, Colorado, USA.
Kashuk
Jeffry L
JL
Moore
Ernest E
EE
Silliman
Christopher C
CC
D000328:Adult; D001792:Blood Platelets / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D012189:Retrospective Studies; D013916:Thrombelastography; D019851:Thrombophilia / Q000175:diagnosis* / Q000201:enzymology / Q000209:etiology*