title: Difference of fibroblast growth factor receptor 1 expression among CA1-3 regions of the gerbil hippocampus after transient cerebral ischemia.
abstract: Fibroblast growth factors are important regulators of neuronal development. In this study, we observed fibroblast growth factor receptor 1 (FGFR1) immunoreactivity and its protein levels in the hippocampus proper (CA1-3 regions) of the gerbil at various time points after ischemia/reperfusion. In the sham-operated group, FGFR1 immunoreaction was not detected in the hippocampus proper. FGFR1 immunoreaction was first detected in non-pyramidal neurons in the CA1-3 region at 12h and 1day after ischemia/reperfusion. From 2days after ischemia/reperfusion, FGFR1 immunoreaction was found in astrocytes, not in microglial cells, in the CA1 region: FGFR1 immunoreactivity and the number of astrocytes were significantly increased at 5days post-ischemia. Western blot analysis revealed that FGFR1 protein levels were also increased from 1day after ischemia/reperfusion. These results indicate that increase of FGFR1 in astrocytes of the ischemic CA1 region may be associated with gliosis followed by delayed neuronal death.
authors.affiliation: Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea.
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Seung-Hae
authors.initials: SH
authors.lastname: Kwon
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.forename: Sang Guan
authors.initials: SG
authors.lastname: You
authors.forename: Young-Myeong
authors.initials: YM
authors.lastname: Kim
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology; D015153:Blotting, Western; D056547:CA1 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D056651:CA2 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D056654:CA3 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death; D003627:Data Interpretation, Statistical; D005849:Gerbillinae; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000473:pathology; D010320:Parvalbumins / Q000378:metabolism; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D010320:Parvalbumins; D051496:Receptor, Fibroblast Growth Factor, Type 1