title: The influence of hepatitis C infection activity on oxidative stress markers and erythropoietin requirement in hemodialysis patients.
abstract: We sought to expose the possible effect of hepatitis C virus (HCV) infection on oxidative stress indicators, nutritional status, and erythropoietin (rHuEPO) requirements in maintenance hemodialysis (MHD) patients. A total of 111 MHD patients (69 males, 42 females; mean age 51.3 +/- 13.0 years; MHD duration 78.5 +/- 52.1 months) and 46 healthy controls were enrolled in the study. We excluded patients with hepatitis B infection or malignancy. Indicators for oxidative status were studied in plasma samples obtained at the beginning of a clinically stable MHD session. Measurements were performed for plasma superoxide dismutase, glutathione peroxidase (antioxidative agents), and malonyldialdehyde (MDA; oxidative agent) by spectrophotometric methods. All patients were analyzed for the presence of anti-HCV; positive patients were also evaluated for the presence of HCV RNA. MHD patients were divided into three groups according to HCV infection status: group I (anti-HCV-positive, HCV-RNA-negative; n = 22); group II (anti-HCV-positive, HCV-RNA-positive; n = 22), and group III (anti-HCV-negative; n = 67). According to the analyses, MHD patients showed higher plasma oxidative stress indicators and lower antioxidative indicator levels compared to controls (P < .0001). MHD patients also displayed lower albumin and higher C-reactive protein (CRP) levels compared to controls (P < .0001). Antioxidant levels were decreased significantly from group I to III (P < .0001). MDA levels significantly increased from group I to III (P < 0.01). HCV-RNA-positive patients showed lowest albumin and highest CRP levels and rHuEPO requirements. Although alanine transferase (ALT) levels were in the normal range, group II patients had significantly higher ALT levels than the other groups (P < .01). In conclusion, we observed negative effects of active HCV infection on oxidative stress and rHuEPO requirements. In contrast, we detected that clinically inactive HCV infection was associated with reduced oxidative stress and rHuEPO requirements compared with active HCV infection and HCV-negative patients.
authors.affiliation: Department of Nephrology, Baskent University Hospital, Ankara, Turkey. emretutal@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: Tutal
authors.forename: S
authors.initials: S
authors.lastname: Sezer
authors.forename: A
authors.initials: A
authors.lastname: Ibis
authors.forename: A
authors.initials: A
authors.lastname: Bilgic
authors.forename: N
authors.initials: N
authors.lastname: Ozdemir
authors.forename: D
authors.initials: D
authors.lastname: Aldemir
authors.forename: M
authors.initials: M
authors.lastname: Haberal
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D002097:C-Reactive Protein / Q000378:metabolism; D004921:Erythropoietin / Q000627:therapeutic use*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006526:Hepatitis C / Q000150:complications* / Q000453:epidemiology; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications / Q000209:etiology / Q000628:therapy / Q000821:virology; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D006435:Renal Dialysis; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D004921:Erythropoietin; D002097:C-Reactive Protein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase