Long-term self-renewable feeder-free human induced pluripotent stem cell-derived neural progenitors.
Human induced pluripotent stem cells (hiPSCs) have led to an important revolution in stem cell research and regenerative medicine. To create patient-specific neural progenitors (NPs), we have established a homogenous, expandable, and self-renewable population of multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented with a combination of factors. The established hiPSC-NPs highly expressed Nestin and Sox1. These NPs were continuously propagated for ~1 year without losing their potential to generate astrocytes, oligodendrocytes, and functional neurons and maintained a stable chromosome number. Voltage clamp analysis revealed outward potassium currents in hiPSC-NPs. The self-renewal characteristic of the NPs was demonstrated by a symmetrical mode of Nestin-positive cell division. Additionally, these hiPSC-NPs can be easily frozen and thawed in the presence of Rho-associated kinase (ROCK) inhibitor without losing their proliferation, karyotype stability, and developmental potential. The characteristics of our generated hiPSC-NPs provide the opportunity to use patient-specific or ready-to-use hiPSC-NPs in future biomedical applications.
Nemati
Shiva
S
Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Hatami
Maryam
M
Kiani
Sahar
S
Hemmesi
Katayoun
K
Gourabi
Hamid
H
Masoudi
Najmehsadat
N
Alaei
Sara
S
Baharvand
Hossein
H
D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D015925:Cryopreservation; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008012:Lidocaine / Q000031:analogs & derivatives / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009543:Nifedipine / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D019789:Tetraethylammonium / Q000494:pharmacology; D014158:Transcription, Genetic
D000943:Antigens, Differentiation; D002121:Calcium Channel Blockers; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D026902:Potassium Channel Blockers; C490906:SOX1 protein, human; D055748:SOXB1 Transcription Factors; C012647:QX-314; D019789:Tetraethylammonium; D008012:Lidocaine; D009543:Nifedipine