Bone marrow stromal cell line promotes the proliferation of mast cell progenitors derived from cord blood CD34+ cells under serum-free conditions with a combination of both cell-cell interaction and soluble factors.
A higher production of functional mast cells (MCs) can be generated by co-culturing cord blood-derived CD34+ cells with a human bone marrow stromal cell line under serum-free conditions supplemented with stem cell factor and IL-6. We addressed the question of whether the higher proliferation of MCs in this co-culture system might be due to the higher proliferation of MC progenitors. The stromal cell line increased the cell numbers of MC progenitors derived from cord blood-derived CD34+ cells, in a combination of cell-cell interactions between stromal cells and CD34+ cells, and as yet unidentified soluble factors derived from stromal cells.
Fujihara
Mitsuhiro
M
Japanese Red Cross, Hokkaido Red Cross Blood Center, Yamanote 2-2, Sapporo, Japan. fujihara@hokkaido.bc.jrc.or.jp
Azuma
Hiroshi
H
Ikeda
Hisami
H
Yamaguchi
Miki
M
Hamada
Hirofumi
H
D018952:Antigens, CD34 / Q000378:metabolism; D002450:Cell Communication; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology; D019089:Stem Cell Factor / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
D018952:Antigens, CD34; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D015850:Interleukin-6; D019089:Stem Cell Factor