Inhibition of human breast cancer cell invasion by siRNA against urokinase-type plasminogen activator.
Urokinase plasminogen activator (uPA) correlates closely with breast cancer metastasis via triggering the degradation of divergent matrix proteins. Here, uPA was selectively knocked down in breast carcinoma MDA-MB-231 cells by siRNAs. The in vitro migration of MDA-MB-231 cells was effectively suppressed accompanied by a decrease in extracellular MMP-9 activities. The colony formation ability of MDA-MB-231 cells was inhibited following uPA knockdown, while the proliferation was not affected. The uPA knockdown in MDA-MB-231 cells caused significantly suppressed tumor metastasis in nude mice. Thus, siRNAs targeted to uPA have implications in the development of novel approaches to preventing breast cancer metastasis.
Huang
Hong-Yan
HY
Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an, China.
Jiang
Ze-Fei
ZF
Li
Qing-Xia
QX
Liu
Jia-Yun
JY
Wang
Tao
T
Zhang
Rui
R
Zhao
Jing
J
Xu
Yan-Ming
YM
Bao
Wei
W
Zhang
Yong
Y
Jia
Lin-Tao
LT
Yang
An-Gang
AG
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D034741:RNA, Small Interfering; D014568:Urokinase-Type Plasminogen Activator