Identification of differentially expressed genes in the brain of Sebastiscus marmoratus in response to tributyltin exposure.
Tributyltin (TBT), a ubiquitous marine environmental contaminant, has been reported to affect functioning of the central nervous system. However, the mechanism of its neurotoxicity remains unknown. In this study, an Anneal Control Primer-differential display Reverse Transcription-PCR method was employed to investigate differentially expressed genes in the brain of Sebastiscus marmoratus in response to acute TBT exposure. A total of 18 gene sequences were identified as having the potential for being differentially expressed, of which 9 could be identified with homologous database sequences. The expression profiles of 4 genes, namely cytochrome c oxidase subunit II, GRB2-associated binding protein 2, adaptor-related protein complex 2, and guanine nucleotide exchange factor p532, were analyzed in the brain using real time fluorescence quantitative PCR after treatment with 10, 100 and 1000 ng/L of TBT for 50 days. The results showed that chronic exposure to TBT induced down-regulation of these genes in a dose dependent manner. The present study provided a basis for studying the response of fish to TBT exposure and allowed the characterization of new potential neurotoxic biomarkers of TBT contamination in seawater.
Li
Bowen
B
Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
Wang
Chonggang
C
Yu
Ang
A
Chen
Yixin
Y
Zuo
Zhenghong
Z
D000818:Animals; D001921:Brain / Q000187:drug effects*; D029941:Fish Proteins / Q000378:metabolism; D005399:Fishes / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D014001:Tin / Q000032:analysis; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D029941:Fish Proteins; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin; D014001:Tin