title: A screening method tuned for mRNA processing factors in human cells by evaluation of the luciferase reporter activity and the subcellular distribution of bulk poly(A)+ RNA.
abstract: Screening of mRNA export factors in Saccharomyces cerevisiae and Drosophila melanogaster has identified a number of mRNA processing factors involved in multiple mRNA processing steps. However, only limited information is available on human cells. Here we established a screening system searching for mRNA processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(A)+ RNA. This system makes it possible to search for the compounds affecting mRNA processing from the various resources.
authors.affiliation: Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University.
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Mayu
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Taiho
authors.initials: T
authors.lastname: Kambe
authors.forename: Masaya
authors.initials: M
authors.lastname: Nagao
authors.forename: Seiji
authors.initials: S
authors.lastname: Masuda
mesh_terms: D017930:Genes, Reporter / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics*; D011061:Poly A / Q000378:metabolism*; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D008156:Luciferases