Effect of acute and chronic administration of methylphenidate on mitochondrial respiratory chain in the brain of young rats.
Methylphenidate is commonly used for the treatment of attention deficit/hyperactivity disorder. There are still few works regarding the effects of methylphenidate on brain energy metabolism. Thus, in the present study we evaluated the effect of chronic administration of methylphenidate on the activities of mitochondrial respiratory chain complexes I and III in the brain of young rats. The effect of acute administration of methylphenidate on mitochondrial respiratory chain complexes I, II, III and IV in the brain of young rats was also investigated. For acute administration, a single injection of methylphenidate was given to rats on postnatal day 25. For chronic administration, methylphenidate injections were given starting at postnatal day 25 once daily for 28 days. Our results showed that complexes I and III were not affected by chronic administration of methylphenidate. Moreover, the acute administration of methylphenidate decreased complex I activity in cerebellum and prefrontal cortex, whereas complexes II, III and IV were not altered.
Fagundes
Ana O
AO
Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
Aguiar
Maira R
MR
Aguiar
Claudia S
CS
Scaini
Giselli
G
Sachet
Monique U
MU
Bernhardt
Nayara M
NM
Rezin
Gislaine T
GT
Valvassori
Samira S
SS
Quevedo
João
J
Streck
Emilio L
EL
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects*; D042967:Electron Transport Complex I / Q000187:drug effects; D014450:Electron Transport Complex III / Q000187:drug effects; D008297:Male; D008774:Methylphenidate / Q000008:administration & dosage* / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D008774:Methylphenidate; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III