A screening method tuned for mRNA processing factors in human cells by evaluation of the luciferase reporter activity and the subcellular distribution of bulk poly(A)+ RNA.
Screening of mRNA export factors in Saccharomyces cerevisiae and Drosophila melanogaster has identified a number of mRNA processing factors involved in multiple mRNA processing steps. However, only limited information is available on human cells. Here we established a screening system searching for mRNA processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(A)+ RNA. This system makes it possible to search for the compounds affecting mRNA processing from the various resources.
Fujiwara
Naoko
N
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University.
Yoshikawa
Mayu
M
Yamazaki
Tomohiro
T
Kambe
Taiho
T
Nagao
Masaya
M
Masuda
Seiji
S
D017930:Genes, Reporter / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics*; D011061:Poly A / Q000378:metabolism*; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D008156:Luciferases