10-23 DNAzyme modified with (2'R)- and (2'S)-2'-deoxy-2'-C-methyluridine in the catalytic core.
The catalytic core of a 10-23 DNAzyme was modified using (2'R), (2'S)-2'-deoxy-2'-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg(2+) concentrations, indicating that certain 2'-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2'-C-methyl-2'-deoxynucleosides increased the stability.
Robaldo
Laura
L
INGEBI (CONICET), Vuelta de Obligado 2490-(1428), Buenos Aires, Argentina.
Montserrat
Javier M
JM
Iribarren
Adolfo M
AM
D020134:Catalytic Domain; D045744:Cell Line, Tumor; D021881:DNA, Catalytic / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
C516788:2'-C-methyluridine; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; C402582:RNA-cleaving DNA 10-23; D008274:Magnesium; D014529:Uridine