Effect of bioaggregate on mineral-associated gene expression in osteoblast cells.
BACKGROUND
This study investigated the cytotoxicity of bioaggregate (BA) and the effect of BA on mineral associated gene expression in osteoblast cells.


METHODS
The cytotoxicity of BA to mouse MC3T3-E1 osteoblast cells was evaluated via the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 1, 2, and 3 days of culture. The expression of mineral associated genes (collagen type 1, osteocalcin, and osteopontin) was assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and compared expression after exposure to BA or mineral trioxide aggregate (MTA). The data were analyzed by one-way analysis of variance and the Tukey test.


RESULTS
BA was essentially nontoxic to osteoblast cells. The expression of collagen type 1, osteocalcin, and osteopontin genes significantly increased in the BA group compared with that in the MTA group on the second or third day of culture.


CONCLUSIONS
BA appears to be a novel nontoxic root-end filling biomaterial and be able to induce mineralization-associated gene expression in osteoblast cells.
Yuan
Zhenglin
Z
State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
Peng
Bin
B
Jiang
Han
H
Bian
Zhuan
Z
Yan
Ping
P
D016475:3T3 Cells; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D004396:Coloring Agents; D004338:Drug Combinations; D017886:Durapatite / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008903:Minerals / Q000032:analysis; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D010087:Oxides / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D024042:Collagen Type I; D004396:Coloring Agents; D004338:Drug Combinations; D008903:Minerals; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C506896:Spp1 protein, mouse; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; D015675:Osteocalcin; D053495:Osteopontin; D017886:Durapatite; C022616:thiazolyl blue; D002126:Calcium Hydroxide; C031293:calcium silicate