title: Oleic acid promotes MMP-9 secretion and invasion in breast cancer cells.
abstract: Epidemiological and animal studies suggest an association between dietary fatty acids and an increase risk of developing breast cancer. Obesity, which is characterized by hyperlipidemia and an elevation of circulating free fatty acids (FFAs), is also associated with enhanced cancer risk. In breast cancer cells, the FFA oleic acid (OA) induces migration, proliferation, prolong survival, invasion, an increase in cellular Ca(2+) concentration, MEK1/2, ERK1/2, FAK and Src activation. However, the role of OA on MMP-9 secretion and invasion has not been studied in detail. We demonstrate here that stimulation of MDA-MB-231 breast cancer cells with 200 μM OA induces an increase on MMP-9 secretion through a PKC, Src, and EGFR-dependent pathway, as revealed by gelatin zymography assays. Furthermore, microtubule network mediates MMP-9 secretion induced by OA. In contrast, OA does not induce an increase on MMP-9 secretion in MCF10A cells, whereas it does not induce MMP-9 secretion in MCF12A mammary non-tumorigenic epithelial cells. In addition, OA induces invasion through an EGFR, Gi/Go proteins, MMPs, PKC and Src-dependent pathway, but it is not able to promote invasion in non-invasive MCF-7 breast cancer cells. In summary, our findings demonstrate that OA promotes an increase on MMP-9 secretion and invasion through a PKC, Src, and EGFR-dependent pathway in breast cancer cells.
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, San Pedro Zacatenco, Mexico, DF, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Soto-Guzman
authors.forename: Napoleon
authors.initials: N
authors.lastname: Navarro-Tito
authors.forename: Luis
authors.initials: L
authors.lastname: Castro-Sanchez
authors.forename: Raul
authors.initials: R
authors.lastname: Martinez-Orozco
authors.forename: Eduardo Perez
authors.initials: EP
authors.lastname: Salazar
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D003599:Cytoskeleton; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D019301:Oleic Acid / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D019301:Oleic Acid; D066246:ErbB Receptors; D011493:Protein Kinase C; D020780:Matrix Metalloproteinase 9