title: A multi-component herbal preparation (PADMA 28) improves structure/function of corticosteroid-treated skin, leading to improved wound healing of subsequently induced abrasion wounds in rats.
abstract: PADMA 28 is a multi-component herbal mixture formulated according to an ancient Tibetan recipe. PADMA 28 is known to stimulate collagen production and reduced levels of collagen-degrading matrix metalloproteinases (MMPs). The goal of the present study was to determine whether topical treatment of rat skin with PADMA 28 would improve skin structure/function, and whether subsequently induced abrasion wounds would heal more rapidly in skin that had been pretreated with PADMA 28. Hairless rats were exposed to a potent topical corticosteroid (Temovate) in combination with either DMSO alone or with PADMA 28 given topically. At the end of the treatment period, superficial wounds were created in the skin, and time to wound closure was assessed. Collagen production and matrix-degrading MMPs were assessed. Abrasion wounds in skin that had been pretreated with PADMA 28 healed more rapidly than did wounds in Temovate plus DMSO-treated skin. Under conditions in which improved wound healing was observed, there was an increased collagen production and decreased MMP expression, but no significant epidermal hyperplasia and no evidence of skin irritation. The ability to stimulate collagen production and inhibit collagen-degrading enzymes in skin and facilitate more rapid wound closure without irritation should provide a rationale for development of the herbal preparation as a "skin-repair" agent.
authors.affiliation: Department of Pathology, The University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Muhammad Nadeem
authors.initials: MN
authors.lastname: Aslam
authors.forename: Roscoe L
authors.initials: RL
authors.lastname: Warner
authors.forename: Narasimharao
authors.initials: N
authors.lastname: Bhagavathula
authors.forename: Isaac
authors.initials: I
authors.lastname: Ginsburg
authors.forename: James
authors.initials: J
authors.lastname: Varani
mesh_terms: D000287:Administration, Topical; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D028321:Plant Preparations; D051381:Rats; D011919:Rats, Inbred Strains; D020127:Recovery of Function / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000305:Adrenal Cortex Hormones; D010936:Plant Extracts; D028321:Plant Preparations; C066069:Padma 28