title: A chromatin-remodeling protein is a component of fission yeast mediator.
abstract: The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex together with Hrp1, a CHD1 ATP-dependent chromatin-remodeling protein. The Med15-Hrp1 subcomplex is not a component of the core Mediator complex but can interact with the L-Mediator conformation. Deletion of med15(+) and hrp1(+) causes very similar effects on global steady-state levels of mRNA, and genome-wide analyses demonstrate that Med15 associates with a distinct subset of Hrp1-bound gene promoters. Our findings therefore indicate that Mediator may directly influence histone density at regulated promoters.
authors.affiliation: From the Division of Metabolic Diseases, Karolinska Institutet, SE-141 86 Stockholm, Sweden.
authors.forename: Olga
authors.initials: O
authors.lastname: Khorosjutina
authors.forename: Paulina H
authors.initials: PH
authors.lastname: Wanrooij
authors.forename: Julian
authors.initials: J
authors.lastname: Walfridsson
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szilagyi
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Zhu
authors.forename: Vera
authors.initials: V
authors.lastname: Baraznenok
authors.forename: Karl
authors.initials: K
authors.lastname: Ekwall
authors.forename: Claes M
authors.initials: CM
authors.lastname: Gustafsson
mesh_terms: D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D016681:Genome, Fungal / Q000502:physiology*; D055106:Genome-Wide Association Study; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D006657:Histones; C552962:Med15 protein, S pombe; D056892:Mediator Complex; D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; C111321:hrp1 protein, S pombe; D004265:DNA Helicases