Mutanase from Paenibacillus sp. MP-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and Streptococcus mutans biofilm.
Laetiporus sulphureus is a source of α-1,3-glucan that can substitute for the commercially-unavailable streptococcal mutan used to induce microbial mutanases. The water-insoluble fraction of its fruiting bodies from 0.15 to 0.2% (w/v) induced mutanase activity in Paenibacillus sp. MP-1 at 0.35 μ ml(-1). The mutanase extensively hydrolyzed streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h. It also degraded α-1,3-polymers of biofilms, formed in vitro by Streptococcus mutans, even after only 3 min of contact.
Pleszczyńska
M
M
Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. mplesz@poczta.onet.pl
Wiater
A
A
Szczodrak
J
J
D018441:Biofilms / Q000187:drug effects*; D055453:Coriolaceae / Q000737:chemistry; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005936:Glucans / Q000302:isolation & purification / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000502:physiology
D005936:Glucans; D011134:Polysaccharides; C045788:alpha-1,3-glucan; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase