Identification of newly described Streptococcus pneumoniae serotype 6D by use of the Quellung reaction and PCR.
We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identify the newly described serotype 6D.
Oftadeh
Shahin
S
Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia. Shahin.oftadeh@swahs.health.nsw.gov.au
Satzke
Catherine
C
Gilbert
Gwendolyn L
GL
D000907:Antibodies, Bacterial; D015373:Bacterial Typing Techniques / Q000379:methods*; D006801:Humans; D011008:Pneumococcal Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000302:isolation & purification*
D000907:Antibodies, Bacterial