Antiedematogenic activity and phytochemical composition of preparations from Echinodorus grandiflorus leaves.
The leaves of Echinodorus grandiflorus (Alismataceae) are traditionally used in Brazil to treat inflammatory conditions. The aim of the present study was to evaluate the antidematogenic activity of crude aqueous, dichloromethane and hydroethanolic extracts from E. grandiflorus leaves using the carrageenan-induced paw edema model in mice, along with of fractions enriched in diterpenes, flavonoids and hydroxycinnamoyltartaric acids (HCTA). Significant inhibitions of paw edema were elicited by the 50% and 70% EtOH extracts (1000 mg/kg, p.o.), as well as by the fractions enriched in diterpenes (70-420 mg/kg, p.o.) and flavonoids (7.2-36 mg/kg, p.o.). Isovitexin, isoorientin, trans-aconitic and chicoric acids were identified in all extracts by HPLC analysis. Trans-aconitic acid itself exhibited significant antiedematogenic effect (270 mg/kg, p.o.). The biological activity correlated positively with the contents of flavonoids and diterpenes, but negatively with HCTA concentrations, demonstrating the participation of the two classes of compounds in the antiedematogenic activity of E. grandiflorus.
Garcia
Eliana de Faria
Ede F
Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
de Oliveira
Mariana Assíria
MA
Godin
Adriana Martins
AM
Ferreira
Wallace Carvalho
WC
Bastos
Leandro Francisco Silva
LF
Coelho
Márcio de Matos
Mde M
Braga
Fernão Castro
FC
D000156:Aconitic Acid / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D034901:Alismataceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D004195:Disease Models, Animal; D004224:Diterpenes / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D004487:Edema / Q000188:drug therapy*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D018515:Plant Leaves
D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D005419:Flavonoids; D010936:Plant Extracts; D002351:Carrageenan; D000156:Aconitic Acid