title: Interleukin-1B and interleukin-1 receptor antagonist gene polymorphisms are not associated with premalignant gastric conditions: a combined haplotype analysis.
abstract: OBJECTIVE
  Contradictory results have been reported about the role of interleukin-1B (IL1B) and IL1 receptor antagonist (IL1RN) alleles in gastric carcinogenesis. Here, IL1B and IL1RN polymorphisms were analyzed as genotypes and haplotypes in relation to the presence of atrophic gastritis (AG) and intestinal metaplasia in the stomach.
  
  
  METHODS
  Two hundred and seventy-eight patients (212 Caucasians and 66 Asians) aged 50 years and above, referred for upper endoscopy because of dyspeptic symptoms, were included in the study. Gastric biopsies were histologically assessed according to the updated Sydney classification. Genomic DNA was typed for polymorphisms at position -3737, -1464, -511, -31 for the IL1B gene and the allele 2 of IL1RN using restriction fragment length polymorphism of amplified PCR fragments and intron-spanning PCR analysis, respectively.
  
  
  RESULTS
  IL1B-1464-C/C genotype was associated with higher presence of AG in antrum of the stomach in Caucasians [odds ratio: 4.8 (95% confidence interval=1.7-14.3); P=0.028]. IL1B-1464-G/C genotype was associated with lower incidence of AG in corpus of the stomach in Asians [odds ratio: 0.7 (95% confidence interval=0.5-0.8); P=0.02]. IL1RN*2 allele was not linked with AG or intestinal metaplasia in all parts of the stomach both among Asians and Caucasians. Overall, data show that none of the major four IL1B polymorphisms (IL1B-3737C>T, -1464G>C, -511C>T, -31T>C) and the IL1RN*2 is individually, or in its haplotype configuration, linked to the presence of premalignant lesions in Caucasians.
  
  
  CONCLUSIONS
  The determination of these IL1-related loci does not have any predictive value for stratification of subgroups with respect to gastric cancer risk.
authors.affiliation: Department of Gastroenterology, Kaunas University of Medicine, Kaunas, Lithuania.
authors.forename: Limas
authors.initials: L
authors.lastname: Kupcinskas
authors.forename: Thomas
authors.initials: T
authors.lastname: Wex
authors.forename: Juozas
authors.initials: J
authors.lastname: Kupcinskas
authors.forename: Marcis
authors.initials: M
authors.lastname: Leja
authors.forename: Audrius
authors.initials: A
authors.lastname: Ivanauskas
authors.forename: Laimas Virgilijus
authors.initials: LV
authors.lastname: Jonaitis
authors.forename: Dainius
authors.initials: D
authors.lastname: Janciauskas
authors.forename: Gediminas
authors.initials: G
authors.lastname: Kiudelis
authors.forename: Konrads
authors.initials: K
authors.lastname: Funka
authors.forename: Agnese
authors.initials: A
authors.lastname: Sudraba
authors.forename: Han-Mo
authors.initials: HM
authors.lastname: Chiu
authors.forename: Jaw-Town
authors.initials: JT
authors.lastname: Lin
authors.forename: Peter
authors.initials: P
authors.lastname: Malfertheiner
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics / Q000706:statistics & numerical data; D005260:Female; D005757:Gastritis, Atrophic / Q000208:ethnology / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D006239:Haplotypes; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011230:Precancerous Conditions / Q000208:ethnology / Q000235:genetics*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics*; D044465:White People / Q000235:genetics / Q000706:statistics & numerical data
chemical_list: C508585:IL1B protein, human; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta