Analysis and stability study of temozolomide using capillary electrophoresis.
The applicability of micellar electrokinetic capillary chromatography (MEKC) for the analysis of temozolomide (TMZ) and its degradants, 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC) and 5-amino-imidazole-4-carboxamide (AIC) has been studied. Using short-end injection, the analysis of TMZ and its degradants could be performed within 1.2 min. The obtained precision of migration times was better than 1.6 RSD%, and the limit of quantitation (LOQ) was 0.31-0.93 microg/mL. The therapeutic concentration of TMZ in blood samples can be determined after direct sample injection and conventional on-capillary UV detection. The proposed MEKC method was applied to study the stability of TMZ in water and serum at different pH values. It was established that the half-life of the TMZ in vitro serum at room temperature was 33 min, close to the half-life (28 min) obtained in water at pH 7.9.
Andrasi
Melinda
M
Department of Inorganic and Analytical Chemistry, University of Debrecen, H-4010 Debrecen, POB. 21., Hungary.
Bustos
Rose
R
Gaspar
Attila
A
Gomez
Frank A
FA
Klekner
Almos
A
D000620:Aminoimidazole Carboxamide / Q000032:analysis / Q000097:blood / Q000737:chemistry; D018906:Antineoplastic Agents, Alkylating / Q000032:analysis / Q000097:blood / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000737:chemistry; D004355:Drug Stability; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000077204:Temozolomide; D013696:Temperature; D014867:Water
D018906:Antineoplastic Agents, Alkylating; D014867:Water; D000620:Aminoimidazole Carboxamide; C027169:5-(3-hydroxymethyl-3-methyl-1-triazeno)imidazole-4-carboxamide; D003606:Dacarbazine; D000077204:Temozolomide