title: Purification and characterization of avian beta-defensin 11, an antimicrobial peptide of the hen egg.
abstract: Natural antimicrobial peptides are present in different compartments (eggshell, egg white, and vitelline membranes) of the hen egg and are expected to be involved in the protection of the embryo during its development and to contribute to the production of pathogen-free eggs. In the present study, we used vitelline membranes from hen (Gallus gallus) eggs as a source of avian β-defensin 11 (AvBD11). A purification scheme using affinity chromatography and reverse-phase chromatography was developed. Purified AvBD11 was analyzed by a combination of mass spectrometry approaches to characterize its primary sequence and structure. A monoisotopic molecular species at [M + H](+) of 9,271.56 Da was obtained, and its N- and C-terminal sequences were determined. We also examined posttranslational modifications and identified the presence of 6 internal disulfide bonds. AvBD11 was found to exhibit antimicrobial activity toward both Gram-positive and Gram-negative bacteria.
authors.affiliation: INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Hervé-Grépinet
authors.forename: Sophie
authors.initials: S
authors.lastname: Réhault-Godbert
authors.forename: Valérie
authors.initials: V
authors.lastname: Labas
authors.forename: Thierry
authors.initials: T
authors.lastname: Magallon
authors.forename: Chrystelle
authors.initials: C
authors.lastname: Derache
authors.forename: Marion
authors.initials: M
authors.lastname: Lavergne
authors.forename: Joël
authors.initials: J
authors.lastname: Gautron
authors.forename: Anne-Christine
authors.initials: AC
authors.lastname: Lalmanach
authors.forename: Yves
authors.initials: Y
authors.lastname: Nys
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D008089:Listeria monocytogenes / Q000187:drug effects; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014817:Vitelline Membrane / Q000737:chemistry; D023083:beta-Defensins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D023083:beta-Defensins