title: Performance of detecting IgM antibodies against enterovirus 71 for early diagnosis.
abstract: Enterovirus 71 (EV71) infection is more likely to induce severe complications and mortality than other enteroviruses. Methods for detection of IgM antibody against EV71 had been established for years, however, the performance of the methods in the very early diagnosis of EV71 infection had not been fully evaluated, which is especially meaningful because of the short incubation period of EV71 infection. In this report, the performance of an IgM anti-EV71 assay was evaluated using acute sera collected from 165 EV71 infected patients, 165 patients infected with other enteroviruses, and more than 2,000 sera from healthy children or children with other infected diseases. The results showed a 90% sensitivity in 20 patients who were in their first illness day, and similar sensitivity remained till 4 days after onset. After then the sensitivity increased to 95% to 100% for more than one month. The specificity of the assay in non-HFMD children is 99.1% (95% CI: 98.6-99.4), similar as the 99.9% specificity in healthy adults. The cross-reaction rate in patients infected with other non-EV71 enteroviruses was 11.4%. In conclusion, the data here presented show that the detection of IgM anti-EV71 by ELISA affords a reliable, convenient, and prompt diagnosis of EV71 infection.
authors.affiliation: National Institute of Diagnostics and Vaccine Development in Infectious Disease, Xiamen University, Xiamen, China.
authors.forename: Feihai
authors.initials: F
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Niu
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Fengcai
authors.initials: F
authors.lastname: Zhu
authors.forename: Shuizhen
authors.initials: S
authors.lastname: He
authors.forename: Shiyin
authors.initials: S
authors.lastname: Zhang
authors.forename: Zuxing
authors.initials: Z
authors.lastname: Weng
authors.forename: Tong
authors.initials: T
authors.lastname: Cheng
authors.forename: Yijun
authors.initials: Y
authors.lastname: Cai
authors.forename: Delei
authors.initials: D
authors.lastname: He
authors.forename: Yixin
authors.initials: Y
authors.lastname: Chen
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Ge
authors.forename: Anthony E T
authors.initials: AE
authors.lastname: Yeo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Mun-Hon
authors.initials: MH
authors.lastname: Ng
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
mesh_terms: D000906:Antibodies / Q000097:blood*; D001483:Base Sequence; D017931:DNA Primers; D042241:Early Diagnosis; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D007075:Immunoglobulin M / Q000276:immunology*; D009500:Neutralization Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D017931:DNA Primers; D007075:Immunoglobulin M