Cross immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus (Boophilus) microplus infestation.
Recombinant Glutathione S-transferase of Haemaphysalis longicornis (rGST-Hl) was expressed in Escherichia coli, purified by affinity chromatography and used in the immunization of cattle. Western blot analysis showed positive antibody response in cattle immunized with rGST-Hl. The tests also showed that immunized bovine sera recognize native Rhipicephalus microplus proteins in different tissue extracts. Furthermore, the vaccine potential of rGST-Hl was investigated against infestation of Hereford cattle by R. microplus. Vaccination of cattle with rGST-Hl conferred partial cross-protection immunity against R. microplus. Considering the effect on number of engorged ticks, egg laying capacity and egg fertility, the overall efficacy of vaccination was of 57%, as compared with control group.
Parizi
Luís Fernando
LF
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500 Prédio 43421, Porto Alegre, RS 91501-970, Brazil.
Utiumi
Kiyoko Uemura
KU
Imamura
Saiki
S
Onuma
Misao
M
Ohashi
Kazuhiko
K
Masuda
Aoi
A
da Silva Vaz
Itabajara
I
D000465:Algorithms; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D026863:Ixodidae / Q000201:enzymology / Q000276:immunology*; D048494:Rhipicephalus / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology
D007106:Immune Sera; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase