Delineating the earliest steps of gilvocarcin biosynthesis: role of GilP and GilQ in starter unit specificity.
In vivo and in vitro investigations of GilP and GilQ, two acyltransferases encoded by the gilvocarcin gene cluster, show that GilQ confers unique starter unit specificity when catalyzing an early as well as rate limiting step of gilvocarcin biosynthesis.
Shepherd
Micah D
MD
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596, USA.
Kharel
Madan K
MK
Zhu
Lili L
LL
van Lanen
Steven G
SG
Rohr
Jürgen
J
D000217:Acyltransferases / Q000378:metabolism*; D003374:Coumarins / Q000737:chemistry*; D006027:Glycosides / Q000096:biosynthesis* / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D048170:Streptomyces lividans / Q000737:chemistry* / Q000201:enzymology*; D013379:Substrate Specificity
D003374:Coumarins; D006027:Glycosides; C031781:gilvocarcin V; D000217:Acyltransferases