Biotransformation of ginsenoside Rb1 to ginsenoside Rd by highly substrate-tolerant Paecilomyces bainier 229-7.
Paecilomyces bainier 229-7 was obtained after UV irradiation for 8 min in the presence of 0.4% LiCl and selection on potato dextrose media containing 30 mg/mL saponin from Panax notoginseng leaves (SPNL). The mutant produces ginsenoside Rd from ginsenoside Rb1 with a bioconversion rate as high as 94.9% under optimized culture conditions in shake flasks when supplied with 20 mg/mL of SPNL. Scale-up in 10-L fermenter resulted in an 89% bioconversion rate. Ginsenoside Rd was purified from the culture medium by a macroporous resin with a chromatographic purity of 92.6%. These results suggest that P. bainier 229-7 could be useful for the preparation of ginsenoside Rd in the pharmaceutical industry.
Ye
Li
L
School of Pharmacy, Fudan University, 826 Zhangheng Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China.
Zhou
Chao-Qun
CQ
Zhou
Wei
W
Zhou
Pei
P
Chen
Dao-Feng
DF
Liu
Xiao-Hui
XH
Shi
Xun-Long
XL
Feng
Mei-Qing
MQ
D001711:Biotransformation; D005285:Fermentation; D036145:Ginsenosides / Q000378:metabolism*; D010143:Paecilomyces / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry
D036145:Ginsenosides; D013501:Surface-Active Agents; C442759:ginsenoside Rb1; C049863:ginsenoside Rd