Induction of interleukin-8 from nasal epithelial cells during bacterial infection: the role of IL-8 for neutrophil recruitment in chronic rhinosinusitis.
OBJECTIVE
The aim of this study was to elucidate the role of IL-8 for neutrophil recruitment in nonallergic CRS patients.


METHODS
After coculture of Streptococcus pneumoniae (SP) with the mucosal epithelial cells (MECs) from non-CRS patients, at three different SP/MEC (1/1, 10/1, 100/1) ratios, the expression of IL-8 mRNA and the concentration of IL-8 were measured by RT-PCR and ELISA. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on endothelial cells and the adherence between neutrophils and human umbilical vascular endothelial cells (HUVECs) were determined by flow cytometric analysis, ELISA, and RIA, respectively.


RESULTS
IL-8 concentration and IL-8 mRNA expression continued to increase from 3 hours after incubation in SP number-dependent manner. The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on HUVECs, and the adherence between neutrophils and HUVECs were significantly increased in 10 SP/MEC-CM, and the increments were significantly blocked by anti-IL-8 antibody.


CONCLUSIONS
MEC and IL-8 are major factors for neutrophil recruitment in nonallergic CRS.
Yoon
Bit-Na
BN
Department of ORL-HNS and Medical Research Institute, Pusan National University School of Medicine, Beomeo-ri, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea.
Choi
Nan-Geum
NG
Lee
Hyun-Sun
HS
Cho
Kyu-Sup
KS
Roh
Hwan-Jung
HJ
D000293:Adolescent; D000328:Adult; D000818:Animals; D001424:Bacterial Infections / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D002478:Cells, Cultured; D002908:Chronic Disease; D004847:Epithelial Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000166:cytology*; D020556:Neutrophil Infiltration / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology / Q000382:microbiology; D012852:Sinusitis / Q000276:immunology / Q000382:microbiology; D055815:Young Adult
D039481:CD11b Antigen; D018821:CD18 Antigens; C509295:ITGAM protein, human; D016209:Interleukin-8