Neurobehavioral deficits in db/db diabetic mice.
Recent clinical studies indicate neurobehavioral disturbances in type-2 diabetics. However, there is paucity of preclinical research to support this concept. The validity of db/db mouse as an animal model to study type-2 diabetes and related complications is known. The present study was designed to investigate comprehensively the db/db mouse behavior as preclinical evidence of type-2 diabetes related major neurobehavioral complications. We tested juvenile (5-6weeks) and adult (10-11weeks) db/db mice for behavioral depression in forced swim test (FST), psychosis-like symptoms using pre-pulse inhibition (PPI) test, anxiety behavior employing elevated plus maze (EPM) test, locomotor behavior and thigmotaxis using open field test and working memory deficits in Y-maze test. Both juvenile and adult group db/db mice displayed behavioral despair with increased immobility time in FST. There was an age-dependent progression of psychosis-like symptoms with disrupted PPI in adult db/db mice. In the EPM test, db/db mice were less anxious as observed by increased percent open arms time and entries. They were also hypo-locomotive as evident by a decrease in their basic and fine movements. There was no impairment of working memory in the Y-maze test in db/db mice. This is the first report of depression, psychosis-like symptoms and anxiolytic behavior of db/db mouse strain. It is tempting to speculate that this mouse strain can serve as useful preclinical model to study type-2 diabetes related neurobehavioral complications.
Sharma
Ajaykumar N
AN
Wright State University, Boonshoft School of Medicine, Department of Pharmacology and Toxicology, Dayton, OH 45435, USA.
Elased
Khalid M
KM
Garrett
Teresa L
TL
Lucot
James B
JB
D000367:Age Factors; D000818:Animals; D001008:Anxiety Disorders / Q000150:complications*; D003866:Depressive Disorder / Q000150:complications*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000503:physiopathology / Q000523:psychology*; D004195:Disease Models, Animal; D004924:Escape Reaction / Q000502:physiology; D005106:Exploratory Behavior / Q000502:physiology; D008297:Male; D016555:Matched-Pair Analysis; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains / Q000523:psychology; D009043:Motor Activity / Q000502:physiology; D017354:Point Mutation; D011618:Psychotic Disorders / Q000150:complications*; D054411:Receptors, Leptin / Q000235:genetics*; D013216:Reflex, Startle / Q000502:physiology
D054411:Receptors, Leptin