title: Inhibition of human breast cancer cell invasion by siRNA against urokinase-type plasminogen activator.
abstract: Urokinase plasminogen activator (uPA) correlates closely with breast cancer metastasis via triggering the degradation of divergent matrix proteins. Here, uPA was selectively knocked down in breast carcinoma MDA-MB-231 cells by siRNAs. The in vitro migration of MDA-MB-231 cells was effectively suppressed accompanied by a decrease in extracellular MMP-9 activities. The colony formation ability of MDA-MB-231 cells was inhibited following uPA knockdown, while the proliferation was not affected. The uPA knockdown in MDA-MB-231 cells caused significantly suppressed tumor metastasis in nude mice. Thus, siRNAs targeted to uPA have implications in the development of novel approaches to preventing breast cancer metastasis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Huang
authors.forename: Ze-Fei
authors.initials: ZF
authors.lastname: Jiang
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Li
authors.forename: Jia-Yun
authors.initials: JY
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Bao
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lin-Tao
authors.initials: LT
authors.lastname: Jia
authors.forename: An-Gang
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000517:prevention & control; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D014568:Urokinase-Type Plasminogen Activator