Functional identification of the Proteus mirabilis core lipopolysaccharide biosynthesis genes.
In this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (LPSs) of two strains of Proteus mirabilis. Since P. mirabilis and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up to the second outer-core residue, the functions of the common P. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of K. pneumoniae. The functions of strain-specific outer-core genes were identified by using as surrogate acceptors LPSs from two well-defined K. pneumoniae core LPS mutants. This approach allowed the identification of two new heptosyltransferases (WamA and WamC), a galactosyltransferase (WamB), and an N-acetylglucosaminyltransferase (WamD). In both strains, most of these genes were found in the so-called waa gene cluster, although one common core biosynthetic gene (wabO) was found outside this cluster.
Aquilini
Eleonora
E
Departamento Microbiología y Parasitología Sanitarias, Facultad de Farmacia, Universidad Barcelona, Av. Joan XXIII s/n, Barcelona, Spain.
Azevedo
Joana
J
Jimenez
Natalia
N
Bouamama
Lamiaa
L
Tomás
Juan M
JM
Regué
Miguel
M
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002240:Carbohydrate Sequence; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D011513:Proteus mirabilis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; C430212:lipopolysaccharide, Proteus; D016695:Glycosyltransferases; D005700:Galactosyltransferases; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; C086735:heptosyltransferase