title: Inhibition of Na currents by the toxin extracts from puffer fishes captured in the sea coast of Japan.
abstract: The inhibitory effects of toxin extracted from muscle or liver of five different puffer fishes (hereafter referred as puffer(s)) captured on the Japanese sea coast were examined on voltage-dependent sodium current (I(Na)) recorded from dissociated single rat hippocampal CA1 neurons. The inhibitory effects estimated from IC(50) values of toxin extracts on I(Na) were in the order of Takifugu vermicularis > Lagocephalus wheeleri > Canthigaster rivulata > Takifugu rubripes > Arothron reticularis from muscle and T. vermicularis > T. rubripes > L. wheeleri > A. reticularis > C. rivulata from liver, thereby indicating that the amount of toxin in the liver or muscle differs between puffers. In addition, the present results indicate that the muscle of T. vermicularis, which is eaten in Japan, contains relatively higher amounts of toxin compared to those of T. rubripes, also eaten. This observation suggests that caution should be taken concerning the maximal edible amount of muscle prepared from T. rubripes.
authors.affiliation: Research Division for Life Sciences, Kumamoto Health Science University, #325 Izumi-machi, Kumamoto city, Kumamoto Pref., 861-5598, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Iwata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Matsuura
authors.forename: Sokatsu
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Tahara
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Shin
authors.forename: Yushi
authors.initials: Y
authors.lastname: Ito
authors.forename: Norio
authors.initials: N
authors.lastname: Akaike
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology; D020128:Inhibitory Concentration 50; D007564:Japan; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D026941:Sodium Channel Blockers / Q000633:toxicity*; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism; D013045:Species Specificity; D023721:Tetraodontiformes / Q000502:physiology*; D013779:Tetrodotoxin / Q000633:toxicity*; D014020:Tissue Extracts / Q000633:toxicity
chemical_list: D026941:Sodium Channel Blockers; D015222:Sodium Channels; D014020:Tissue Extracts; D013779:Tetrodotoxin