Modulation of the reaction of vascular smooth muscle cells to angiotensin II induced by catalase and aminotriasol during ischemia-reperfusion.
BACKGROUND
We investigated the influence of catalase and aminotriasol on reactions of the smooth muscle cells induced by angiotensin II (ANG II) after ischemia-reperfusion (I/R).


METHODS
Experiments were performed on perfused male Wistar rat tail arteries. Using classical pharmacometric methods we analyzed the influence of ANG II on vascular contraction, in the presence of catalase and aminotriazole, and after I/R.


RESULTS
A reduction in maximal response and increased EC(5) value were observed after ischemia, while an increased maximal response and decrease EC(50) value were observed after reperfusion. Catalase decreased and aminotriasol increased maximal responses to ANG II. In the presence of catalase, reduction of the maximal response and increase in EC(50) value were observed after reperfusion. In the presence of aminotriasol, we observed increased maximal response and decreased EC(50) value after I/R.


CONCLUSIONS
Ischemia reduced and reperfusion increased the responses of vascular smooth muscle cells to ANG II. Catalase decreased and aminotriasol increased hyperreactivity of arteries to ANG II after reperfusion. These results suggested that antioxidative system modulates reactions induced by ANG II. Reperfusion impairs the balance between antioxidants and the production of reactive oxygen species.
Szadujkis-Szadurska
K
K
Department of Pharmacology, Nicolaus Copernicus University, Bydgoszcz, Poland.
Slupski
M
M
Szadujkis-Szadurski
R
R
Jasinski
M
M
Grześk
G
G
Matusiak
G
G
D000640:Amitrole / Q000627:therapeutic use*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000502:physiology*; D002374:Catalase / Q000494:pharmacology; D007511:Ischemia / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000517:prevention & control*; D013623:Tail / Q000098:blood supply
D000804:Angiotensin II; D002374:Catalase; D000640:Amitrole