Osteoclast and osteoblast activities on carbonate apatite plates in cell cultures.
Previous studies have demonstrated that carbonate apatite (CA) is superior to hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP) with regard to osteoclastic resorption, but evidence on osteoclast and osteoblast response remains controversial. In the present study, the expression of bone related mRNA is examined on CA, HA, β-TCP, and titanium plates. ICR mouse osteoblast cells are cocultured with ICR mouse bone marrow cells. Crude osteoclast-like cell-rich suspensions are then seeded onto plates and cultured for 48 h. Total RNA is extracted and mRNA expression is examined by real-time RT-PCR. Amounts of vacuolar-type ATPase, cathepsin K, and TRAP mRNA are significantly greater on CA than on the other plates. The amount of osteoprotegerin mRNA is significantly greater on CA than on the other plates. RANKL mRNA expression, which is generally regarded as an osteoblast maker, varies with material, but shows no significant differences between CA and the other plates. The formation and activity of osteoclasts is greater with CA than with the other plates. Thus, CA is superior to β-TCP as a bioresorbable bone substitute for tissue engineering.
Kanayama
Keiichi
K
Department of Periodontology, Division of Oral Infections and Health Science, Asahi University School of Dentistry, 1851, Hozumi, Mizuho, Gifu 501-0296, Japan. k-k1@dent.asahi-u.ac.jp
Sriarj
Wantida
W
Shimokawa
Hitoyata
H
Ohya
Keiichi
K
Doi
Yutaka
Y
Shibutani
Toshiaki
T
D000135:Acid Phosphatase / Q000235:genetics; D000818:Animals; D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D056657:Cathepsin K / Q000235:genetics; D018929:Cell Culture Techniques; D024042:Collagen Type I / Q000235:genetics; D017886:Durapatite; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D053244:Osteoprotegerin / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D023822:Tissue Engineering; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics
D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; D007527:Isoenzymes; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; C485817:beta-tricalcium phosphate; C030782:carboapatite; D017886:Durapatite; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D056657:Cathepsin K; C534132:Ctsk protein, mouse; D025262:Vacuolar Proton-Translocating ATPases