Efficacy of frog skin lipids in wound healing.
BACKGROUND
Frog skin has been sequentially and scientifically evaluated by our group for its wound healing efficiency. Owing to the complex structure of skin, attempts were being made to analyse the role of individual constituents in different phases of healing. Our earlier papers have shown the significance of frog skin not only in wound healing but also enhancing the proliferating activity of the epidermal and dermal cells which are instrumental for normal healing process. We also have identified for the first time novel antimicrobial peptides from the skin of Rana tigerina and thereby reduce the complications involved in the sepsis.
The current study envisages the role of frog skin lipids in the inflammatory phase of wound healing. The lipid moiety of the frog skin dominated by phospholipids exhibited a dose dependent acceleration of healing irrespective of the mode of application. The efficiency of the extract is attributed partially to the anti-inflammatory activity as observed by the histochemical and immunostimulatory together with plethysmographic studies.


CONCLUSIONS
Thus, frog skin for the first time has been demonstrated to possess lipid components with pharmaceutical and therapeutic potential. The identification and characterization of such natural healing molecules and evaluating their mechanism of action would therefore provide basis for understanding the cues of Nature and hence can be used for application in medicine.
Raghavan K
Venkat
V
Department of Biomaterials, Central Leather Research Institute, Chennai, India.
Babu
Mary
M
Rajaram
Rama
R
Purna Sai
Korrapati
K
D000279:Administration, Cutaneous; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D006097:Granulation Tissue / Q000737:chemistry / Q000187:drug effects; D006967:Hypersensitivity / Q000188:drug therapy / Q000276:immunology; D056724:Immunity, Humoral / Q000187:drug effects; D007194:India; D007274:Injections, Intraperitoneal; D008055:Lipids / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology / Q000627:therapeutic use*; D008419:Materia Medica; D008519:Medicine, Traditional; D011898:Ranidae; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry* / Q000187:drug effects* / Q000293:injuries; D014020:Tissue Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008055:Lipids; D008419:Materia Medica; D014020:Tissue Extracts