title: Crystal structure of the Mg·ADP-inhibited state of the yeast F1c10-ATP synthase.
abstract: The F(1)c(10) subcomplex of the yeast F(1)F(0)-ATP synthase includes the membrane rotor part c(10)-ring linked to a catalytic head, (αβ)(3), by a central stalk, γδε. The Saccharomyces cerevisiae yF(1)c(10)·ADP subcomplex was crystallized in the presence of Mg·ADP, dicyclohexylcarbodiimide (DCCD), and azide. The structure was solved by molecular replacement using a high resolution model of the yeast F(1) and a bacterial c-ring model with 10 copies of the c-subunit. The structure refined to 3.43-Å resolution displays new features compared with the original yF(1)c(10) and with the yF(1) inhibited by adenylyl imidodiphosphate (AMP-PNP) (yF(1)(I-III)). An ADP molecule was bound in both β(DP) and β(TP) catalytic sites. The α(DP)-β(DP) pair is slightly open and resembles the novel conformation identified in yF(1), whereas the α(TP)-β(TP) pair is very closed and resembles more a DP pair. yF(1)c(10)·ADP provides a model of a new Mg·ADP-inhibited state of the yeast F(1). As for the original yF(1) and yF(1)c(10) structures, the foot of the central stalk is rotated by ∼40 ° with respect to bovine structures. The assembly of the F(1) central stalk with the F(0) c-ring rotor is mainly provided by electrostatic interactions. On the rotor ring, the essential cGlu(59) carboxylate group is surrounded by hydrophobic residues and is not involved in hydrogen bonding.
authors.affiliation: Université Bordeaux 2, CNRS, Institut de Biochimie et Génétique Cellulaires, 1 rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France. A.Dautant@ibgc.cnrs.fr
authors.forename: Alain
authors.initials: A
authors.lastname: Dautant
authors.forename: Jean
authors.initials: J
authors.lastname: Velours
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Giraud
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry*; D001386:Azides / Q000737:chemistry; D018360:Crystallography, X-Ray; D004024:Dicyclohexylcarbodiimide / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D001386:Azides; D029701:Saccharomyces cerevisiae Proteins; D004024:Dicyclohexylcarbodiimide; D000244:Adenosine Diphosphate; D008274:Magnesium