Discrimination of saturated aldehydes by the rat I7 olfactory receptor.
The discrimination of n-alkyl-saturated aldehydes during the early stage of odorant recognition by the rat I7 olfactory receptor (OR-I7) is investigated. The concentrations of odorants necessary for 50% activation (or inhibition) of the OR-I7 are measured by calcium imaging recordings of dissociated rat olfactory sensory neurons, expressing the recombinant OR-I7 from an adenoviral vector. These are correlated with the corresponding binding free energies computed for a homology structural model of the OR-I7 built from the crystal structure of bovine visual rhodopsin at 2.2 A resolution.
Kurland
Michael D
MD
Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.
Newcomer
Michael B
MB
Peterlin
Zita
Z
Ryan
Kevin
K
Firestein
Stuart
S
Batista
Victor S
VS
D000447:Aldehydes / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004192:Discrimination, Psychological / Q000187:drug effects; D008958:Models, Molecular; D009812:Odorants; D051381:Rats; D018035:Receptors, Odorant / Q000737:chemistry / Q000502:physiology*; D012243:Rhodopsin / Q000737:chemistry; D011984:Sensory Receptor Cells
D000447:Aldehydes; D018035:Receptors, Odorant; C509506:olfactory receptor I7, rat; D012243:Rhodopsin