title: Amino acid sequence of myoglobin from emu (Dromaius novaehollandiae) skeletal muscle.
abstract: The objective of the present study was to characterize the primary structure of emu myoglobin (Mb). Emu Mb was isolated from Iliofibularis muscle employing gel-filtration chromatography. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry was employed to determine the exact molecular mass of emu Mb in comparison with horse Mb, and Edman degradation was utilized to characterize the amino acid sequence. The molecular mass of emu Mb was 17,380 Da and was close to those reported for ratite and poultry myoglobins. Similar to myoglobins from meat-producing livestock and birds, emu Mb has 153 amino acids. Emu Mb contains 9 histidines. Proximal and distal histidines, responsible for coordinating oxygen-binding property of Mb, are conserved in emu. Emu Mb shared more than 90% homology with ratite and chicken myoglobins, whereas it demonstrated only less than 70% sequence similarity with ruminant myoglobins.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA. spsuma2@email.uky.edu
authors.forename: S P
authors.initials: SP
authors.lastname: Suman
authors.forename: P
authors.initials: P
authors.lastname: Joseph
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: C M
authors.initials: CM
authors.lastname: Beach
authors.forename: M
authors.initials: M
authors.lastname: Fontaine
authors.forename: L
authors.initials: L
authors.lastname: Steinke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D020312:Dromaiidae; D006639:Histidine / Q000032:analysis; D006736:Horses; D058751:Livestock; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis* / Q000302:isolation & purification; D018482:Muscle, Skeletal / Q000737:chemistry*; D009211:Myoglobin / Q000032:analysis* / Q000302:isolation & purification; D046108:Palaeognathae; D012418:Ruminants; D017386:Sequence Homology, Amino Acid
chemical_list: D009124:Muscle Proteins; D009211:Myoglobin; D006639:Histidine