Neuroprotective effects of tamoxifen on experimental spinal cord injury in rats.
The aim of this study was to evaluate the effects of tamoxifen on tumor necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) levels and ultrastructural changes in rats with spinal cord injury. Rats were divided into four groups: control group (laminectomy only), trauma group (laminectomy+spinal trauma), tamoxifen group (laminectomy+spinal trauma+tamoxifen), and vehicle group (laminectomy+spinal trauma+vehicle). Spinal cords were extirpated at the T(7)-T(12) level and tissue samples from the spinal cords were gathered for TNF-alpha and IL-1beta measurements at 1 and 6hours. Spinal cords harvested at 6 hours were evaluated for ultrastructural changes. TNF-alpha and IL-1beta levels at 6 hours were significantly lower in the tamoxifen group than in the trauma group. Electron microscopic examination of tissue from the trauma group revealed gross cell deformities with widespread edema of all structures as well as severe edema in the neuropil. At 6 hours after trauma, these ultrastructural changes were less marked in the tamoxifen group. Our findings support a neuroprotective and restorative role for tamoxifen in the context of secondary pathological biochemical events after SCI.
Ismailoğlu
Ozgür
O
Department of Neurosurgery, School of Medicine, Süleyman Demirel University, Cünür, Isparta 32260, Turkey. ozguri_36@hotmail.com
Oral
Baha
B
Görgülü
Aşkin
A
Sütçü
Recep
R
Demir
Necdet
N
D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D053583:Interleukin-1beta / Q000378:metabolism; D007796:Laminectomy / Q000009:adverse effects; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission / Q000379:methods; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013116:Spinal Cord / Q000473:pathology / Q000648:ultrastructure; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018709:Statistics, Nonparametric; D013629:Tamoxifen / Q000627:therapeutic use*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D053583:Interleukin-1beta; D018696:Neuroprotective Agents; D014409:Tumor Necrosis Factor-alpha; D013629:Tamoxifen