title: Development of a certified reference material for the determination of acrylamide in potato chips.
abstract: A certified reference material (CRM), KRISS CRM 108-10-003, has been developed for analysis of acrylamide in potato chips, as a representative of carbohydrate-rich food cooked in high-temperature oil. The material was prepared by grinding commercially available potato chips to a paste which was then homogenized, bottled in 15-g units, and stored at -70 °C. Certification, homogeneity and stability testing, were carried out by liquid chromatography-isotope-dilution mass spectrometry (ID-LC-MS). A single ID-LC-MS measurement was performed for each of 10 selected units for certification and homogeneity assessment. The mean measurement result for the 10 bottles, 0.455 ± 0.012 mg kg(-1), was assigned as the certified value of the CRM. The between-bottle homogeneity was 0.8% of the certified value. The within-bottle homogeneity, tested by measuring three replicate sub-samples from each of three randomly selected bottles, was similar to the between-bottle homogeneity. The stability of the CRM under storage conditions (-70 °C) was tested for 21 months and no change in the acrylamide content was observed within the measurement uncertainty. Stability of the CRM at -20 °C (storage at user's site) and room temperature (for regular use and transportation) was also tested. Also presented is the newly designed procedure for evaluating the uncertainty of the certified value for the characterization scheme used in this study.
authors.affiliation: Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Yuseong, Daejon, 305-600, Korea. byungjoo@kriss.re.kr
authors.forename: Byungjoo
authors.initials: B
authors.lastname: Kim
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Park
authors.forename: Insun
authors.initials: I
authors.lastname: Lee
authors.forename: Youngran
authors.initials: Y
authors.lastname: Lim
authors.forename: Euijin
authors.initials: E
authors.lastname: Hwang
authors.forename: Hun-Young
authors.initials: HY
authors.lastname: So
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D057140:Fast Foods / Q000032:analysis*; D005504:Food Analysis / Q000379:methods / Q000592:standards*; D005506:Food Contamination / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards; D012015:Reference Standards; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D020106:Acrylamide