Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis.
Treatment of Linum album cell cultures with 10 μM salicylic acid (SA) for 3 days improved podophyllotoxin (PTOX) production up to 333 μg/g dry weight (DW): over three times that of the control cultures. qPCR analyses showed that in SA-treated cells, the expression of the genes coding for phenylalanine ammonia-lyase (PAL), cinnamoyl-CoA reductase (CCR) and cinnamyl-alcohol dehydrogenase (CAD), all involved in the first steps of PTOX biosynthesis, also increased reaching a peak 8-12 h after the treatment. Expression of the pinoresinol-lariciresinol reductase gene (PLR), which is involved in one of the last biosynthetic steps, was not affected by SA. The selective action of SA on these genes can be applied to control the biotechnological production of this anticancer agent.
Yousefzadi
Morteza
M
Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, PO Box 14115-154, Tehran, Iran.
Sharifi
Mozafar
M
Behmanesh
Mehrdad
M
Ghasempour
Alireza
A
Moyano
Elisabeth
E
Palazon
Javier
J
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis; D053898:Biosynthetic Pathways; D018929:Cell Culture Techniques; D019597:Flax / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D010088:Oxidoreductases / Q000096:biosynthesis; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis; D010940:Plant Proteins / Q000096:biosynthesis*; D011034:Podophyllotoxin / Q000096:biosynthesis*; D020156:Salicylic Acid / Q000378:metabolism*
D010940:Plant Proteins; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C084969:pinoresinol reductase; C018656:cinnamyl alcohol dehydrogenase; D000445:Aldehyde Oxidoreductases; C019904:cinnamoyl CoA reductase; D010650:Phenylalanine Ammonia-Lyase; D011034:Podophyllotoxin; D020156:Salicylic Acid