title: Recognition of potentially novel human disease-associated pathogens by implementation of systematic 16S rRNA gene sequencing in the diagnostic laboratory.
abstract: Clinical isolates that are difficult to identify by conventional means form a valuable source of novel human pathogens. We report on a 5-year study based on systematic 16S rRNA gene sequence analysis. We found 60 previously unknown 16S rRNA sequences corresponding to potentially novel bacterial taxa. For 30 of 60 isolates, clinical relevance was evaluated; 18 of the 30 isolates analyzed were considered to be associated with human disease.
authors.affiliation: University of Zurich, Institute of Medical Microbiology, Gloriastrasse 30/32, CH-8006 Zurich, Switzerland.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Keller
authors.forename: Silvana K
authors.initials: SK
authors.lastname: Rampini
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Büchler
authors.forename: Gerhard
authors.initials: G
authors.lastname: Eich
authors.forename: Roger M
authors.initials: RM
authors.lastname: Wanner
authors.forename: Roberto F
authors.initials: RF
authors.lastname: Speck
authors.forename: Erik C
authors.initials: EC
authors.lastname: Böttger
authors.forename: Guido V
authors.initials: GV
authors.lastname: Bloemberg
mesh_terms: D000328:Adult; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S