title: In vitro mutagenicity assessment of aluminium oxide nanomaterials using the Salmonella/microsome assay.
abstract: The aim of the current study was to evaluate the potential mutagenicity of aluminium oxide nanomaterials (NMs) (Al(2)O(3)-30 nm and Al(2)O(3)-40 nm). Characterization of the NMs was done before the initiation of the study. The mutagenicity of the NMs was studied by the Ames test with Salmonella typhimurium TA100, TA1535, TA98, TA97a and TA102 strains, in the presence and absence of the S9 mixture. Based on a preliminary cytotoxicity study conducted on the strains, different concentrations of Al(2)O(3)-30 nm, Al(2)O(3)-40 nm and Al(2)O(3)-bulk were selected. At all the concentrations tested, Al(2)O(3)-30 nm and Al(2)O(3)-40 nm did not significantly increase the number of revertant colonies compared to the Al(2)O(3)-bulk and control with or without S9 mixture. Our findings suggest that Al(2)O(3) NMs were devoid of any size and concentration dependent mutagenicity compared to the Al(2)O(3)-bulk and control.
authors.affiliation: Toxicology Unit, Biology Division, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh, India.
authors.forename: A
authors.initials: A
authors.lastname: Balasubramanyam
authors.forename: N
authors.initials: N
authors.lastname: Sailaja
authors.forename: M
authors.initials: M
authors.lastname: Mahboob
authors.forename: M F
authors.initials: MF
authors.lastname: Rahman
authors.forename: Saber M
authors.initials: SM
authors.lastname: Hussain
authors.forename: Paramjit
authors.initials: P
authors.lastname: Grover
mesh_terms: D000537:Aluminum Oxide / Q000145:classification / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002458:Cell Fractionation; D053768:Metal Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008862:Microsomes, Liver; D009153:Mutagens / Q000009:adverse effects* / Q000145:classification / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000187:drug effects* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D009153:Mutagens; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D000537:Aluminum Oxide