Multicomponent mesofluidic system for the detection of veterinary drug residues based on competitive immunoassay.
An automated multicomponent mesofluidic system (MCMS) based on biorecognitions carried out on meso-scale glass beads in polydimethylsiloxane (PDMS) channels was developed. The constructed MCMS consisted of five modules: a bead introduction module, a bioreaction module, a solution handling module, a liquid driving module, and a signal collection module. The integration of these modules enables the assay to be automated and reduces it to a one-step protocol. The MCMS has successfully been applied toward the detection of veterinary drug residues in animal-derived foods. The drug antigen-coated beads (varphi250 microm) were arrayed in the PDMS channels (varphi300 microm). The competitive immunoassay was then carried out on the surface of the glass beads. After washing, the Cy3-labeled secondary antibody was introduced to probe the antigen-antibody complex anchored to the beads. The fluorescence intensity of each bead was measured and used to determine the residual drug concentration. The MCMS is highly sensitive, with its detection limits ranging from 0.02 (salbutamol) to 3.5 microg/L (sulfamethazine), and has a short assay time of 45 min or less. The experimental results demonstrate that the MCMS proves to be an economic, efficient, and sensitive platform for multicomponent detection of compound residues for contamination in foods or the environment.
Hu
Lei
L
Lab of Biosystems and Microanalysis Laboratory, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Zuo
Peng
P
Ye
Bang-Ce
BC
D000420:Albuterol / Q000032:analysis; D000906:Antibodies / Q000276:immunology; D001331:Automation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D007118:Immunoassay / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods; D013418:Sulfamethazine / Q000032:analysis; D013997:Time Factors; D019155:Veterinary Drugs / Q000032:analysis*
D000906:Antibodies; D004129:Dimethylpolysiloxanes; D019155:Veterinary Drugs; D013418:Sulfamethazine; C013830:baysilon; D000420:Albuterol