MEKC determination of IgG in human serum via a pH-mediated acid stacking method.
An on-column preconcentration technique, pH-mediated acid stacking, was used in this study to improve the sensitivity of MEKC-UV analysis of IgG in human serum. Various parameters affecting pH-mediated acid stacking were optimized systematically. To eliminate the matrix interferences of human serum and to combine the sample pretreatment procedure with the detection methodology, silica-coated Fe(3)O(4) magnetic nanoparticles modified with N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane were prepared and employed as solid phase extraction adsorbent to remove the abundant HSA from human serum. HSA was quantitatively removed by silica-coated Fe(3)O(4) magnetic nanoparticles modified with N-(2-aminoethyl)-3-aminopropyl-trimethoxysilanes without retaining IgG at pH 9.3. Under the optimum conditions, the sensitivity of IgG was improved 40.3-fold using a 100 s electrokinetic injection as compared with a 6 s hydrodynamic injection. The detection limit of IgG was found to be 0.1 mg/L, and the proposed method was successfully applied for the determination of IgG in human serum with satisfactory results.
Wu
Yi-Wei
YW
Department of Chemistry and Environmental Engineering, Hubei Normal University, Huangshi, PR China. chemwuyiwei@tom.com
Liu
Jun-Feng
JF
Deng
Zhen-Li
ZL
Zhang
Jing
J
Jiang
Feng
F
Xiong
Kun
K
Zhang
Haili
H
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry; D052616:Solid Phase Extraction
D005290:Ferric Compounds; D007074:Immunoglobulin G; C000499:ferric oxide