title: Biochemical characterisation of Murray Valley encephalitis virus proteinase.
abstract: Murray Valley encephalitis virus (MVEV) is a member of the flavivirus group, a large family of single stranded RNA viruses, which cause serious disease in all regions of the world. Its genome encodes a large polyprotein which is processed by both host proteinases and a virally encoded serine proteinase, non-structural protein 3 (NS3). NS3, an essential viral enzyme, requires another virally encoded protein cofactor, NS2B, for proteolytic activity. The cloning, expression and biochemical characterisation of a stable MVEV NS2B-NS3 fusion protein is described.
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*Star), Singapore, Singapore.
authors.forename: Joma
authors.initials: J
authors.lastname: Joy
authors.forename: Ng Fui
authors.initials: NF
authors.lastname: Mee
authors.forename: Wee Liang
authors.initials: WL
authors.lastname: Kuan
authors.forename: Kwek Zekui
authors.initials: KZ
authors.lastname: Perlyn
authors.forename: Then Siew
authors.initials: TS
authors.lastname: Wen
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hill
mesh_terms: D018068:Encephalitis Virus, Murray Valley / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005416:Flavivirus / Q000235:genetics / Q000378:metabolism*; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D057057:Serine Proteases; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020815:Polyproteins; D014764:Viral Proteins; D010450:Endopeptidases; D057057:Serine Proteases