title: RamA and RamB are global transcriptional regulators in Corynebacterium glutamicum and control genes for enzymes of the central metabolism.
abstract: In Corynebacterium glutamicum, the transcriptional regulators of acetate metabolism RamA (encoded by cg2831) and RamB (encoded by cg0444) play an important role in expression control of genes involved in acetate and ethanol metabolism. Both regulators were speculated to have broader significance in expression control of further genes in the central metabolism of C. glutamicum. Here we investigated the RamA and RamB regulons by genome-wide transcriptome analysis with special emphasis on genes encoding enzymes of the central carbon metabolism. When compared to the parental wild-type, 253 genes and 81 genes showed different mRNA levels in defined RamA- and RamB-deficient C. glutamicum strains, respectively. Among these were genes involved in sugar uptake, glycolysis, gluconeogenesis, acetate, l-lactate or ethanol metabolism. The direct interaction of RamA and RamB proteins with the respective promoter/operator fragments was demonstrated in vitro by electrophoretic mobility shift assays. Taken together, we present evidence for an important role of RamA and RamB in global gene expression control in C. glutamicum.
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Auchter
authors.forename: Annette
authors.initials: A
authors.lastname: Cramer
authors.forename: Andrea
authors.initials: A
authors.lastname: Hüser
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.forename: Denise
authors.initials: D
authors.lastname: Emer
authors.forename: Peggy
authors.initials: P
authors.lastname: Schwarz
authors.forename: Annette
authors.initials: A
authors.lastname: Arndt
authors.forename: Christian
authors.initials: C
authors.lastname: Lange
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Volker F
authors.initials: VF
authors.lastname: Wendisch
authors.forename: Bernhard J
authors.initials: BJ
authors.lastname: Eikmanns
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D013455:Sulfur / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D005227:Fatty Acids; D014157:Transcription Factors; D013455:Sulfur; D002244:Carbon; D005947:Glucose