Enzymes of naphthalene metabolism by Pseudomonas fluorescens 26K strain.
The ability of Pseudomonas fluorescens 26K strain to utilize naphthalene at concentrations up to 600 mg/liter as the sole source of carbon and energy in mineral liquid media was shown. Using HPLC, TLC, and mass-spectrometry, the intermediates of naphthalene transformation by this strain were identified as naphthalene cis-1,2-dihydrodiol, salicylaldehyde, salicylate, catechol, 2-hydroxymuconic semialdehyde, and 1-naphthol. Catechol 2,3-dioxygenase (a homotetramer with native molecular mass 125 kDa) and NAD+-dependent homohexameric naphthalene cis-1,2-dihydrodiol dehydrogenase with native molecular mass 160 kDa were purified from crude extract of the strain and characterized. NAD+-dependent homodimeric salicylaldehyde dehydrogenase with molecular mass 110 kDa was purified and characterized for the first time. Based on the data, a pathway of naphthalene degradation by P. fluorescens 26K is suggested.
Leneva
N A
NA
Pushchino State University, Pushchino, Moscow Region, 142292, Russia.
Kolomytseva
M P
MP
Baskunov
B P
BP
Golovleva
L A
LA
D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D000447:Aldehydes / Q000032:analysis; D050557:Catechol 2,3-Dioxygenase / Q000737:chemistry* / Q000302:isolation & purification; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D013058:Mass Spectrometry; D009281:Naphthalenes / Q000378:metabolism*; D009284:Naphthols / Q000032:analysis; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry* / Q000302:isolation & purification; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D012459:Salicylates / Q000032:analysis
D000447:Aldehydes; D002396:Catechols; D005231:Fatty Acids, Unsaturated; D009281:Naphthalenes; D009284:Naphthols; D012459:Salicylates; C013243:salicylaldehyde; C029350:1-naphthol; C002091:hydroxymuconic semialdehyde; C042334:1,2-dihydroxy-1,2-dihydronaphthalene; D050557:Catechol 2,3-Dioxygenase; D000445:Aldehyde Oxidoreductases; C024246:salicylaldehyde dehydrogenase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C020054:cis-1,2-dihydrobenzene-1,2-diol dehydrogenase; C034221:catechol