title: Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8.
abstract: Aquaporin (AQP) family plays a fundamental role in transmembrane water and small solutes movement. Within this family, aquaporin 8 (AQP8), showed to be widely distributed in the digestive system especially colon. To investigate the possible protein alterations involved in AQP8 regulation and trafficking, we extensively compared between wild type and AQP8 knockout mouse colon using semi-quantitative fluorescence- stained two dimensional gel electrophoresis (2-DE) coupled with nano LC-Ms/Ms. Our analysis revealed identification and regulation of 21 proteins, most notably, actin-related family which suggests its possible involvement in regulating AQP8 secretory vesicles migration to be integrated as a cell membrane protein.
authors.affiliation: Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-dori, Japan. samehmagd@med.niigata-u.ac.jp
authors.forename: Sameh
authors.initials: S
authors.lastname: Magdeldin
authors.forename: Huiping
authors.initials: H
authors.lastname: Li
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Shymaa
authors.initials: S
authors.lastname: Enany
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Fujinaka
authors.forename: Eishin
authors.initials: E
authors.lastname: Yaoita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics*; D003106:Colon / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D007525:Isoelectric Focusing; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020543:Proteome / Q000378:metabolism
chemical_list: D020346:Aquaporins; D020543:Proteome; C108330:aquaporin 8