title: Feeding response of Aedes aegypti and Anopheles dirus (Diptera: Culicidae) using out-of-date human blood in a membrane feeding apparatus.
abstract: The colonization of Aedes aegypti and Anopheles dirus was performed using out-of-date human blood from a blood bank as a nutritional supply dispensed from a common artificial feeder. Preserved human blood was collected and used for feeding on days 5, 15, and 25 after date of expiration and dispensed from a common artificial feeder to rear the mosquitoes. Ae. aegypti had a feeding rate of 78.7, 62, and 18% at the respective intervals while An. dirus had a rate of 80, 56.8, and 7.3% on the same respective days. Direct feeding on live hamsters resulted in a rate of 96 and 90% for Ae. aegypti and An. dirus, respectively. Although egg production rates decreased from the day 5 feeding to the day 25 feeding, all of the developmental stages resulting from An. dirus fed at day 5 and 15 showed insignificant differences when compared with direct feeding on the blood of a hamster.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, and Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: Jinrapa
authors.initials: J
authors.lastname: Pothikasikorn
authors.forename: Rapee
authors.initials: R
authors.lastname: Boonplueang
authors.forename: Chalermchai
authors.initials: C
authors.lastname: Suebsaeng
authors.forename: Rungpetch
authors.initials: R
authors.lastname: Khaengraeng
authors.forename: Theeraphap
authors.initials: T
authors.lastname: Chareonviriyaphap
mesh_terms: D000330:Aedes / Q000502:physiology*; D000818:Animals; D000852:Anopheles / Q000502:physiology*; D001769:Blood; D005247:Feeding Behavior / Q000502:physiology*; D006801:Humans