title: Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate.
abstract: With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer-ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
authors.forename: Subash C B
authors.initials: SC
authors.lastname: Gopinath
authors.forename: Ramanujam
authors.initials: R
authors.lastname: Kumaresan
authors.forename: Koichi
authors.initials: K
authors.lastname: Awazu
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Mizuhata
authors.forename: Junji
authors.initials: J
authors.lastname: Tominaga
authors.forename: Penmetcha K R
authors.initials: PK
authors.lastname: Kumar
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009696:Nucleic Acids / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D009696:Nucleic Acids; D011506:Proteins; D006046:Gold; D012825:Silicon