[The mutations of the D-loop hypervariable region II and hypervariable region III of mitochondrial DNA in oral squamous cell carcinoma].
OBJECTIVE
To investigate the frequency of mitochondrial DNA (mtDNA) D-loop hypervariable region II (HVR II) and hypervariable region III (HVR III) mutations in oral squamous cell carcinoma (OSCC) and their correlation to provide the new targets for the prevention and treatment of OSCC.


METHODS
The D-loop HVR II and HVR III regions of mtDNA in seven cases with OSCC tissues, matched with paracancerous tissues and normal mucosa tissues from the same case, were amplified by polymerase chain raction (PCR), then were detected by direct sequencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.


RESULTS
82 (56 species) nucleotide changes, with 51(26 species) nucleotide polymorphism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database. 31(30 species) mutations, with 21 located within the HVR II and HVR III regions, were found in 3 tumor tissue samples, their paracancerous and normal mucosa tissue were found more polymorphic changes but no mutation. The mtDNA D-loop HVR II and HVR III regions mutation rate was 42.9% (3/7) in OSCC.


CONCLUSIONS
The mtDNA D-loop HVR II and HVR III regions were highly polymorphic and mutable regions in OSCC. It suggested that the D-loop HVR II and HVR III regions of mtDNA might play a significant role in the tumorigenesis of OSCC. It may become new targets for the gene therapy of OSCC by regulating the above indexes.
Wang
Yao-Zhong
YZ
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China.
Jia
Mu-Yun
MY
Yuan
Rong-Tao
RT
Han
Guo-Dong
GD
Bu
Ling-Xue
LX
D002294:Carcinoma, Squamous Cell; D004272:DNA, Mitochondrial; D005260:Female; D006801:Humans; D009062:Mouth Neoplasms; D009154:Mutation; D011110:Polymorphism, Genetic
D004272:DNA, Mitochondrial