Randomized comparison of different ovarian stimulation regimens for assisted reproductive technology in baboons (Papio anubis).
OBJECTIVE
To compare different methods of ovarian stimulation (OS) for assisted reproductive technology in baboons.


METHODS
Prospective randomized study.


METHODS
Institute of primate research.


METHODS
Baboons (n = 10) were randomized into two groups (of five animals each) during three different cycles to compare six protocols of OS.


METHODS
Cycle 1: clomiphene citrate (CC) alone (group CC) versus CC and GnRH agonist (group CC-Ag); cycle 2: recombinant gonadotropins (GON) without GnRH agonist (group GON) versus GON and depot GnRH agonist (group GON-AgDepo-1); cycle 3: GON and depot GnRH agonist (group GON-AgDepo-2) versus GON and daily GnRH agonist in a classic long protocol (group GON-Ag). Oocyte aspiration was performed 34-36 hours after injecting 5,000 IU rhCG, followed by fertilization via intracytoplasmic sperm injection (ICSI).


METHODS
Number and quality of oocytes retrieved and their fertilization rate.


RESULTS
More metaphase II (MII) oocytes were retrieved using the GON-AgDepo-1 (n = 12; 64% MII), GON-AgDepo-2 (n = 9; 79% MII), GON-Ag (n = 16; 88% MII), and GON (n = 6; 59% MII) protocols compared with the CC (n = 9; 15% MII) and CC-Ag (n = 14; 20% MII) protocols. Fertilization by ICSI varied between 43% and 71%.


CONCLUSIONS
In baboons, long and depot protocols yield similar numbers of MII oocytes; however, depot protocol may be preferable because only one injection of GnRH agonist is needed.
Nyachieo
Atunga
A
Leuven University Fertility Center, University Hospital Gasthuisberg, Leuven, Belgium.
Spiessens
Carl
C
Chai
Daniel C
DC
Debrock
Sophie
S
Mwenda
Jason M
JM
d'Hooghe
Thomas M
TM
D000818:Animals; D003692:Delayed-Action Preparations; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000819:agonists; D008297:Male; D008597:Menstrual Cycle / Q000187:drug effects / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D048530:Papio anubis / Q000502:physiology*; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted
D003692:Delayed-Action Preparations; D007987:Gonadotropin-Releasing Hormone