Rotationally induced hydrodynamics: fundamentals and applications to high-speed bioassays.
Bioassays are indispensable tools in areas ranging from fundamental life science research to clinical practice. Improving assay speed and levels of detection will have a profound impact in all of these areas. We recently developed a rapid, sensitive format for immunosorbent assays that expedites antigen mass transport by rotating the capture substrate. This review outlines the theoretical foundation of rotationally induced hydrodynamics and its application in heterogeneous assays. We describe a general solution that solves the rates of immunoreactions on rotating capture substrates, taking into account both diffusion and the rate of reaction between antibody and antigen. The general solution applies to a wide range of rotation rates, including mass transport-limited to reaction rate-limited assays, and is validated experimentally. We discuss several applications that demonstrate how immunoassays can be tailored to increase speed as well as lower the limit of detection of viral particles, pathogens, toxins, and proteins.
Wang
Gufeng
G
Institute for Physical Research and Technology, U.S. Department of Energy, Iowa State University, Ames, 50011, USA.
Driskell
Jeremy D
JD
Hill
April A
AA
Dufek
Eric J
EJ
Lipert
Robert J
RJ
Porter
Marc D
MD
D001681:Biological Assay / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D012399:Rotation; D013059:Spectrum Analysis, Raman
D006046:Gold