Up-regulation of licochalcone A biosynthesis and secretion by Tween 80 in hairy root cultures of Glycyrrhiza uralensis Fisch.
We evaluated the effect of Tween 80 as elicitor on licochalcone A from hairy root cultures of Glycyrrhiza uralensis Fisch. After a 15-days treatment with 2% Tween 80, hairy roots still grew well and produced higher levels of licochalcone A and total flavonoids than the control (without treatment). Licochalcone A content and total flavonoid content were 3.103 and 127.095 mg per flask (9- and 11-fold higher), respectively, compared with controls. Secretion of licochalcone A and total flavonoids into the culture medium was remarkably high, up to 98 and 94% of the total production, respectively. The enhanced flavonoid production was associated with elevated mRNA levels and enzyme activities of phenylalanine ammonia-lyase (PAL), 4-coumarate:coenzyme A ligase (4CL), and cinnamate-4-hydroxylase (C4H). These results clearly demonstrated that Tween 80 treatment permeabilized the roots to enhance secretion, but also acted as an efficient elicitor of licochalcone A and total flavonoid production in hairy roots of G. uralensis Fisch.
Zhang
Hai-Chao
HC
Department of Breeding and Genetics, China Pharmaceutical University, 210038, Nanjing, Jiangsu Province, China.
Liu
Jing-Mei
JM
Chen
Hai-Min
HM
Gao
Chun-Chun
CC
Lu
Hong-Yu
HY
Zhou
Hua
H
Li
Yi
Y
Gao
Shan-Lin
SL
D047188:Chalcones / Q000096:biosynthesis* / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D029882:Glycyrrhiza uralensis / Q000254:growth & development / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry*; D015854:Up-Regulation
D047188:Chalcones; D003470:Culture Media; D011136:Polysorbates; D010650:Phenylalanine Ammonia-Lyase; D003066:Coenzyme A Ligases; C070840:licochalcone A