title: Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coli O157:H7 in cattle.
abstract: BACKGROUND
  Escherichia coli serogroup O157:H7 has emerged as an important zoonotic bacterial pathogen, causing a range of symptoms from self-limiting bloody diarrhea to severe hemorrhagic colitis and hemolytic-uremic syndrome in humans. Beef and dairy cattle are considered the most important animal reservoirs for this pathogen. One of the important virulence characteristics of E. coli O157:H7 is the eaeA gene encoding the 97 kDa surface protein intimin. Intimin is required for attachment and effacement during the interaction of enterohemorrhagic E. coli with human and bovine neonatal enterocytes. The present study was undertaken to test the hypothesis that an adaptive mucosal immune response directed against intimin will reduce or prevent enteric colonization and fecal shedding of E. coli O157:H7 in cattle.
  
  
  RESULTS
  Cattle were orally inoculated with either milk (control), milk with live attenuated Salmonella enterica serovar Dublin (vector), or milk with live attenuated recombinant S. Dublin expressing intimin (vaccinated) on days 0, 14 and 28. On day 98, all calves were challenged orally with E. coli O157:H7 to evaluate whether vaccination with the recombinant S. Dublin expressing intimin would reduce the level of E. coli O157:H7 fecal shedding.During the first 28 days, vaccinated calves shed both the vector strain and the intimin-expressing S. Dublin strain at a similar level. The vector strain was shed for a significantly longer period as compared to the level of recombinant vaccine strain. Calves that received the intimin-expressed vaccine ceased shedding S. Dublin from day 28 to day 63. All calves were challenged with E. coli O157:H7 on day 98 to determine the effect on fecal shedding of E. coli O157:H7. The amount of E. coli O157:H7 in feces was measured for 30 days post-challenge. We observed a transient clearance of E. coli O157:H7 from the feces in the vaccinated calves. The magnitude of fecal E. coli O157:H7 shedding did not correlate with the presence of intimin-specific fecal IgA.
  
  
  CONCLUSIONS
  Oral vaccination with live attenuated recombinant S. Dublin expressing intimin reduced enteric colonization and fecal shedding of E. coli O157:H7. However, the transient clearance of E. coli O157:H7 was not associated with an enhanced IgA-mediated mucosal immune response.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843 USA. skhare@cvm.tamu.edu
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Khare
authors.forename: Walid
authors.initials: W
authors.lastname: Alali
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhang
authors.forename: Doris
authors.initials: D
authors.lastname: Hunter
authors.forename: Roberta
authors.initials: R
authors.lastname: Pugh
authors.forename: Ferric C
authors.initials: FC
authors.lastname: Fang
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Libby
authors.forename: L Garry
authors.initials: LG
authors.lastname: Adams
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology; D000818:Animals; D000833:Animals, Suckling; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D003001:Cloning, Molecular / Q000379:methods; D015169:Colony Count, Microbial / Q000662:veterinary; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000276:immunology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000592:standards; D005243:Feces / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005822:Genetic Vectors / Q000494:pharmacology; D007070:Immunoglobulin A / Q000097:blood; D008297:Male; D019779:Salmonella enterica / Q000235:genetics; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology* / Q000592:standards; D015047:Zoonoses / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D007070:Immunoglobulin A; D014614:Vaccines, Synthetic; C073819:eaeA protein, E coli