Proliferative effects of chishao on injured peripheral neurons.
The aim of the study was to evaluate the proliferative effects of chishao on neuron regeneration. A silicone rubber nerve guide across a 15-mm gap was filled with different concentrations of chishao (0-125 mg/ml) in the dissected sciatic nerve of the right leg in SD rats. The left legs were used as control. After eight weeks, the regenerated nerves showed dose-dependently activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA), decreased plasminogen activator inhibitor-1 (PAI-1) and enhanced proliferative proteins, extracellular signal regulated kinase (ERK)- and c-Jun N-terminal kinase (JNK)-signalings. The results imply that applying an appropriate dose of chishao would be a potential approach for enhancing neuron regeneration.
Fang
Wen-Kuei
WK
Department of Neurosurgery, Chia-Yi Christian Hospital, Taiwan.
Weng
Yi-Jiun
YJ
Chang
Mu-Hsin
MH
Lin
Chien-Chung
CC
Chen
Yueh-Sheng
YS
Hsu
Hsi-Hsien
HH
Tsai
Fuu-Jen
FJ
Tsai
Chang-Hai
CH
Kuo
Wu-Hsien
WH
Lu
Chung Yen
CY
Huang
Chih-Yang
CY
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D029596:Paeonia; D008517:Phytotherapy; D018517:Plant Roots; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D017395:Plasminogen Activator Inhibitor 1; D016222:Fibroblast Growth Factor 2; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D014568:Urokinase-Type Plasminogen Activator