title: Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form).
abstract: An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form) followed by gel-permeation chromatography. The polysaccharide was subjected to Smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing L-threonine. The initial and modified polysaccharides were studied by sugar analysis and 1H- and 13C-NMR spectroscopy, including COSY, TOCSY, ROESY, and HSQC experiments, and the structure of the branched tetrasaccharide repeating unit of the polysaccharide was established.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia. komand@piboc.dvo.ru
authors.forename: N A
authors.initials: NA
authors.lastname: Komandrova
authors.forename: V V
authors.initials: VV
authors.lastname: Isakov
authors.forename: S V
authors.initials: SV
authors.lastname: Tomshich
authors.forename: L A
authors.initials: LA
authors.lastname: Romanenko
authors.forename: A V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
mesh_terms: D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D031161:Pseudoalteromonas / Q000737:chemistry*; D013912:Threonine / Q000737:chemistry
chemical_list: D019081:O Antigens; D013912:Threonine