Association between functional promoter polymorphisms of macrophage migration inhibitory factor (MIF) gene and ulcerative colitis in Japan.
Macrophage migration inhibitory factor (MIF) is a key proinflammatory mediator. Two functional polymorphisms were identified in the promoter region of MIF gene. We attempted to clarify the associations between these polymorphisms and ulcerative colitis (UC). The study was performed in 111 patients with UC and 209 subjects without UC. We employed the PCR-SSCP method to detect gene polymorphisms. Overall, 5/5-CATT genotype was a decreased risk for the development of UC (OR, 0.51; 95% CI, 0.26-0.99). In addition, 7/7-CATT genotype was significantly associated with chronic continuous phenotype and distal colitis phenotype (OR, 5.49; 95% CI, 1.19-25.3, and OR, 6.10; 95% CI, 1.32-28.2, respectively), whereas 5/5-CATT genotype had an inhibitory effect on the development of UC after 20years of age (OR, 0.33; 95% CI, 0.14-0.82). On the other hand, G-173C polymorphism did not affect the susceptibility to and the phenotypes of UC. Our results suggested that tetranucleotide CATT repeat of MIF gene promoter may be associated with the development of UC and the severity of inflammation in patients with UC.
Shiroeda
Hisakazu
H
Department of Gastroenterology, Kanazawa Medical University, School of Medicine, 1-1, Daigaku, Uchinada-machi, Ishikawa 920-0293, Japan.
Tahara
Tomomitsu
T
Nakamura
Masakatsu
M
Shibata
Tomoyuki
T
Nomura
Tomoe
T
Yamada
Hideto
H
Hayashi
Ranji
R
Saito
Takashi
T
Yamada
Masayoshi
M
Fukuyama
Tomoki
T
Otsuka
Toshimi
T
Yano
Hirokazu
H
Ozaki
Kazuaki
K
Tsuchishima
Mutsumi
M
Tsutsumi
Mikihiro
M
Arisawa
Tomiyasu
T
D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007249:Inflammation / Q000235:genetics; D007564:Japan; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic
D008263:Macrophage Migration-Inhibitory Factors