title: Complex rearrangement of chromosomes 1, 7, 21, 22 in Ewing sarcoma.
abstract: The Ewing sarcoma (ES) family of tumors is characterized by nonrandom chromosomal translocations involving the EWSR1 gene on chromosome 22 with one of the members of the ETS family of transcription factors. The majority of ES tumors are characterized by a balanced translocation t(11;22)(q24;q12), which results in the fusion of the 5' portion of EWSR1 gene with the 3'end of the FLI1 gene. Fusions with ERG, another member of the ETS family, occur in less than 10% of ES tumors, and can arise through complex chromosomal rearrangements. Here, we report a case of a 5-year-old female with an ES tumor in the thoracic region. G-banding and spectral karyotyping analysis demonstrated 46,XX,t(1;21;7)(q25;q22.3;q22). Metaphase fluorescence in situ hybridization (FISH) using the EWSR1 break-apart probe demonstrated a normal signal on both apparently normal chromosomes 22, and an additional EWSR1-5' signal on the derivative chromosome 21. Reverse-transcriptase polymerase chain reaction analysis of RNA isolated from the tumor demonstrated a EWSR1-ERG fusion transcript, fusing exon 7 of EWSR1 and exon 11 of ERG. These results are consistent with an additional copy of the 5' portion of EWSR1, which inverted and then inserted on chromosome 21 and fused to the 3' end of ERG. To our knowledge, this is the first report of a EWSR1-ERG fusion in an ES tumor with an apparently duplicated 5' portion of EWSR1, and with a complex translocation involving chromosomes 1, 7, and 21. This case adds to the spectrum of genetic rearrangements identified in ES tumors.
authors.affiliation: Institute of Genetic Medicine, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, MD 21287, USA.
authors.forename: Natini
authors.initials: N
authors.lastname: Jinawath
authors.forename: Laura
authors.initials: L
authors.lastname: Morsberger
authors.forename: Alexis
authors.initials: A
authors.lastname: Norris-Kirby
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Williams
authors.forename: Raluca
authors.initials: R
authors.lastname: Yonescu
authors.forename: Pedram
authors.initials: P
authors.lastname: Argani
authors.forename: Constance A
authors.initials: CA
authors.lastname: Griffin
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Murphy
mesh_terms: D001483:Base Sequence; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D002675:Child, Preschool; D002871:Chromosome Banding; D002877:Chromosomes, Human; D017931:DNA Primers; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000235:genetics*; D032681:Spectral Karyotyping; D014178:Translocation, Genetic
chemical_list: D002148:Calmodulin-Binding Proteins; D017931:DNA Primers; C500986:EWSR1 protein, human; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins