Photothermolysis of glioblastoma stem-like cells targeted by carbon nanotubes conjugated with CD133 monoclonal antibody.
CD133(+) cells in glioblastoma (GBM) display cancer stem cell-like properties and have been considered as the culprit of tumor recurrence, justifying exploration of potential therapeutic modalities targeting CD133(+) cancer stem-like cells (CSCs). For photothermolysis studies, GBM-CD133(+) and GBM-CD133(-) cells mixed with various ratios were challenged with single-walled carbon nanotubes (SWNTs) conjugated with CD133 monoclonal antibody (anti-CD133) and then irradiated with near-infrared laser light. Results show that GBM-CD133(+) cells were selectively targeted and eradicated, whereas GBM-CD133(-) cells remained viable. In addition, in vitro tumorigenic and self-renewal capability of GBM-CD133(+) treated with localized hyperthermia was significantly blocked. Furthermore, GBM-CD133(+) cells pretreated with anti-CD133-SWNTs and irradiated by near-infrared laser 2 days after xenotransplantation in nude mice did not exhibit sustainability of CSC features for tumor growth. Taken altogether, our studies demonstrated that anti-CD133-SWNTs have the potential to be utilized as a thermal-coupling agent to effectively target and destroy GBM CSCs in vitro and in vivo.


BACKGROUND
Glioblastoma remains one of the most notorious cancer from the standpoint of recurrence and overall resistance to therapy. CD133+ stem cells occur among GBM cells, and may be responsible for the huge recurrence risk. This paper discusses a targeted elimination method of these cells, which may enable more efficient therapy in an effort to minimize or prevent recurrence.
Wang
Chung-Hao
CH
Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
Chiou
Shih-Hwa
SH
Chou
Chen-Pin
CP
Chen
Yu-Chih
YC
Huang
Yao-Jhang
YJ
Peng
Ching-An
CA
D000071916:AC133 Antigen; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D005909:Glioblastoma / Q000628:therapy*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D037742:Nanotubes, Carbon / Q000737:chemistry*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010455:Peptides / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006023:Glycoproteins; D037742:Nanotubes, Carbon; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse