[Interleukin-27 affects IFN-gamma and IL-4 activities in children with asthma].
OBJECTIVE
To study the effect of interleukin (IL)-27 on INF and IL-4 activities in children with asthma.


METHODS
The levels of IFN-gamma and IL-4 in culture supernatants of peripheral blood mononuclear cells (PBMCs) from 23 asthmatic children were measured using ELISA. PBMCs were cultured with recombinant IL-27 (5 ng/mL or 0.5 ng/mL) for 12 hrs in vitro.


RESULTS
IFN-gamma levels in both 5 ng/mL and 0.5 ng/mL IL-27 treatment groups (85.9+/-12.2 and 8.9+/-2.3 microg/L, respectively) increased compared with those in the cytokine stimulated control group (P<0.01, P<0.05 respectively). The group treated with 5 ng/mL IL-27 had decreased significantly IL-4 levels compared with the cytokine stimulated control group (15.0+/-1.9 microg/L vs 77.0+/-15.6 microg/L; P<0.01).


CONCLUSIONS
IL-27 may be involved in the pathogenesis of asthma. It may affect Th1/Th2 cell activities and might be a new option for the treatment of asthma.
Chen
Jie
J
Clinical Medical School of Yangtze University, Jingzhou, Hubei 434000, China.
Yuan
Yue-Sha
YS
Li
Qi
Q
Liu
Mei
M
Zhou
Shao-Hua
SH
Jin
Wei
W
D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis*; D020381:Interleukin-17 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology
D020381:Interleukin-17; D011994:Recombinant Proteins; D015847:Interleukin-4; D007371:Interferon-gamma