New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction coupled to fast gas chromatography/negative chemical-electron ionization/mass spectrometry.
The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.
Pacenti
Marco
M
Occupational Health Division, Department of Public Health, Viale Morgagni 48, University of Florence, 50100 Florence, Italy.
Dugheri
Stefano
S
Traldi
Pietro
P
Degli Esposti
Filippo
F
Perchiazzi
Nicola
N
Franchi
Elena
E
Calamante
Massimo
M
Kikic
Ireneo
I
Alessi
Paolo
P
Bonacchi
Alice
A
Salvadori
Edoardo
E
Arcangeli
Giulio
G
Cupelli
Vincenzo
V