Production of cytidine 5'-diphosphorylcholine with high utilization of ATP by whole cells of Saccharomyces cerevisiae.
Cytidine 5'-diphosphorylcholine (CDP-choline) was produced using a high efficiency ATP regeneration system and the Kennedy pathway in whole cells of Saccharomyces cerevisiae As 2.398. Out of eight variables, KH(2)PO(4), glycerol and (NH(4))(2)SO(4) were considered to be the most significant factors by response surface methodology including a Plackett-Burman design, path of steepest accent and central composite design. The optimum levels of the three variables were 20.13g/L KH(2)PO(4), 12.35g/L glycerol and 0.49g/L (NH(4))(2)SO(4), respectively. Energy utilization efficiency increased from 10.59% to 16.72% and choline chloride conversion yields increased from 12.35% to 42.78%. A high efficiency ATP regeneration system improves CDP-choline production.
Tang
Jiapeng
J
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, PR China.
Chen
Yong
Y
Chen
Xiaochun
X
Yao
Yuelan
Y
Ying
Hanjie
H
Xiong
Jian
J
Bai
Jianxin
J
D000255:Adenosine Triphosphate / Q000378:metabolism*; D003566:Cytidine Diphosphate Choline / Q000138:chemical synthesis*; D008657:Metabolic Clearance Rate; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D003566:Cytidine Diphosphate Choline; D000255:Adenosine Triphosphate