title: Quantitative DIC microscopy using an off-axis self-interference approach.
abstract: Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self-interference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to a quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirements on polarization optics. Optical sectioning can be obtained through enlarged illumination NA.
authors.affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. danfu@mit.edu
authors.forename: Dan
authors.initials: D
authors.lastname: Fu
authors.forename: Seungeun
authors.initials: S
authors.lastname: Oh
authors.forename: Wonshik
authors.initials: W
authors.lastname: Choi
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Yamauchi
authors.forename: August
authors.initials: A
authors.lastname: Dorn
authors.forename: Zahid
authors.initials: Z
authors.lastname: Yaqoob
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Michael S
authors.initials: MS
authors.lastname: Feld
mesh_terms: D000465:Algorithms; D003952:Diagnostic Imaging; D005069:Evaluation Studies as Topic; D006367:HeLa Cells; D006696:Holography / Q000379:methods; D006801:Humans; D008027:Light; D008029:Lighting; D008857:Microscopy, Interference / Q000379:methods*