High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor.
A common method to minimize overflow metabolism and to enable high cell-density is to operate microbial processes in fed-batch mode under carbon-limiting conditions. This requires sophisticated process control schemes with expensive hardware equipment and software and well-characterized processes parameters. To generate high-cell density, a more simplified strategy would be beneficial. Therefore, a genetically engineered Escherichia coli strain with a modified glucose uptake system was cultivated in batch mode. In the applied strain, the usual phosphotransferase system of a K12-derived strain was inactivated, while the galactose permease system was amplified. Upon cultivating this E. coli strain in pure minimal media, the acetate concentration did not exceed values of 0.35 g L(-1), even when the batch fermentation was started with a glucose concentration of 130 g L(-1). Finally, maximum biomass concentrations of 48 g L(-1) dry cell weight and maximum space-time yields of 2.10 g L(-1) h(-1) were reached. To provide an unlimited growth under fully aerobic conditions (DOT>30%) at comparatively low values for specific power input (3-4 kW m(-3)), a pressurized bioreactor was used. Consequentially, to our knowledge, this study using a bioreactor with elevated headspace pressure generate the highest oxygen transfer rate (451 mmol L(-1) h(-1)) ever reached in batch cultivations.
Knabben
Ingo
I
RWTH Aachen, AVT--Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany.
Regestein
Lars
L
Marquering
Frank
F
Steinbusch
Sven
S
Lara
Alvaro R
AR
Büchs
Jochen
J
D000085:Acetates / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D011312:Pressure; D013696:Temperature
D000085:Acetates; D005947:Glucose