The requirement of natural killer T-cells in tolerogenic APCs-mediated suppression of collagen-induced arthritis.
TGF-beta-induced tolerogenic-antigen presenting cells (Tol-APCs) could induce suppression of autoimmune diseases such as collagen-induced arthritis (CIA) and allergic asthma. In contrast, many studies have shown that NKT cells are involved in the pathogenesis of Th1-mediated autoimmune joint inflammation and Th2-mediated allergic pulmonary inflammation. In this study, we investigated the effect of CD1d-restricted NKT cells in the Tol-APCs-mediated suppression of autoimmune disease using a murine CIA model. When CIA-induced mice were treated with Tol-APCs obtained from CD1d+/- or CD1d-/- mice, unlike CD1d+/- APCs, CD1d-/- Tol-APCs failed to suppress CIA. More specifically, CD1d-/- Tol-APCs failed to suppress the production of inflammatory cytokines and the induction of Th2 responses by antigen-specific CD4 T cells both in vitro and in vivo. Our results demonstrate that the presence of CD1d-restricted NKT cells is critical for the induction of Tol-APCs-mediated suppression of CIA.
Jung
Sundo
S
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Park
Yoon-Kyung
YK
Shin
Jung Hoon
JH
Lee
Hyunji
H
Kim
Soo-Young
SY
Lee
Gap Ryol
GR
Park
Se-Ho
SH
D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology*; D055610:Antigens, CD1d / Q000276:immunology; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology* / Q000517:prevention & control*; D024043:Collagen Type II / Q000276:immunology; D016207:Cytokines / Q000097:blood; D007108:Immune Tolerance / Q000276:immunology*; D018836:Inflammation Mediators / Q000097:blood; D051379:Mice; D055611:Natural Killer T-Cells / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
D000906:Antibodies; D055610:Antigens, CD1d; D024043:Collagen Type II; D016207:Cytokines; D018836:Inflammation Mediators