[Antitumor effect by combination of recombinant endostatin adenovirus with carboplatin].
OBJECTIVE
To investigate the antitumor effect of recombinant Endostatin adenovirus (Ad-E) with carboplatin in mice with Lewis lung cancer.


METHODS
C57BL/6 mice bearing Lewis lung cancer received Ad-E and carboplatin respectively or in combination. The tumor volume, tumor net weight, survival time and side effects were observed. Histological analysis was conducted to assess microvessel density (MVD) within tumor tissue and apoptotic cells were identified using the TUNEL assay.


RESULTS
Compared with the control groups, the combination treatment significantly suppressed the tumor growth and prolonged survival time of the mice without obvious side-effects. The histological analysis revealed the combination treatment led to a decreased MVD and increased percent apoptosis in LL/2 tumors.


CONCLUSIONS
Our studies indicate that a combination of Ad-E with carboplatin can present better antitumor effects without obvious side-effect.
Liao
Jia-Qun
JQ
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Su
Xiao-Qing
XQ
Peng
Xing-Chen
XC
Shi
Hua-Shan
HS
Zhang
Hai-Long
HL
Yang
Li
L
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D016190:Carboplatin / Q000627:therapeutic use*; D018827:Carcinoma, Lewis Lung / Q000628:therapy*; D003131:Combined Modality Therapy; D043169:Endostatins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use
D000970:Antineoplastic Agents; D043169:Endostatins; D011994:Recombinant Proteins; D016190:Carboplatin