title: Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.
abstract: OBJECTIVE
  To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models.
  
  
  METHODS
  Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation.
  
  
  METHODS
  Collaborative investigation in an academic research environment.
  
  
  METHODS
  Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control.
  
  
  METHODS
  Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls.
  
  
  METHODS
  Mitochondrial protein expression.
  
  
  RESULTS
  Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%.
  
  
  CONCLUSIONS
  We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis.
authors.affiliation: Department of Gynecology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ding
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Yuefang
authors.initials: Y
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D016022:Case-Control Studies; D003944:Diagnostic Techniques, Obstetrical and Gynecological; D004715:Endometriosis / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014591:Uterine Diseases / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D024101:Mitochondrial Proteins