Enhancement of thermal stabilization of formaldehyde dehydrogenase from Pseudomonas putida by directed evolution.
We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 degrees C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 degrees C), the variants obtained retained <80% activity for at least 500 h (21 d).
Imamura
Chie
C
Toyota Central R&D Labs., Inc., Aichi-gun, Aichi.
Shigemori
Yasushi
Y
D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019020:Directed Molecular Evolution; D004789:Enzyme Activation; D004795:Enzyme Stability; D016958:Pseudomonas putida / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent