title: Diverse mechanisms of endothelium-derived hyperpolarizing factor-mediated dilatation in small myometrial arteries in normal human pregnancy and preeclampsia.
abstract: This ex vivo study focuses on the mechanisms of endothelium-dependent dilatation in the uterine circulation of normal pregnancy (n = 12) and in women with preeclampsia (n = 12). Arteries (internal diameter, ∼250 μm) isolated by myometrial biopsy from women undergoing planned cesarean delivery or delivery as a result of the deterioration of preeclampsia were studied using a wire myograph. Bradykinin-induced dilatation was assessed in the presence and/or absence of pharmacological inhibitors to determine the contribution of nitric oxide and endothelium-derived hyperpolarizing factor (EDHF), as well as that of EDHF-mediated pathways such as myoendothelial gap junctions (MEGJs) and products of arachidonic acid, H(2)O(2) and cytochrome P450 2C9 (CYP2C9). Transmission electron microscopy was used to visualize morphological prerequisites for MEGJs. In normal pregnancy, EDHF through MEGJs appeared to be a predominant mediator conferring endothelium-dependent relaxation in small myometrial arteries. In preeclampsia, bradykinin-induced relaxation was reduced via compromised EDHF-type responses, in which the contribution of MEGJs became negligible. The attenuated role of MEGJs to endothelium-dependent relaxation was partly compensated through the contribution of H(2)O(2) or other endothelium-derived relaxing factors. CYP2C9 products of arachidonic acid had no effect on EDHF-type relaxation in arteries of women with normal pregnancy or with preeclampsia. We suggest that EDHF-type responses via MEGJs are primarily targeted in small myometrial arteries in women with preeclampsia. This could significantly contribute to the impaired uteroplacental blood flow in this disorder.
authors.affiliation: Institution of Clinical Science, Technology and Intervention, Department of Obstetrics and Gynecology, Karolinska Institute, Karolinska University Hospital, Huddinge Campus, Stockholm, Sweden.
authors.forename: Leanid
authors.initials: L
authors.lastname: Luksha
authors.forename: Natallia
authors.initials: N
authors.lastname: Luksha
authors.forename: Marius
authors.initials: M
authors.lastname: Kublickas
authors.forename: Henry
authors.initials: H
authors.lastname: Nisell
authors.forename: Karolina
authors.initials: K
authors.lastname: Kublickiene
mesh_terms: D000328:Adult; D001158:Arteries / Q000187:drug effects / Q000503:physiopathology*; D001685:Biological Factors / Q000037:antagonists & inhibitors / Q000502:physiology*; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure; D005260:Female; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D009215:Myometrium / Q000098:blood supply*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009994:Osmolar Concentration; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D055815:Young Adult
chemical_list: D001685:Biological Factors; D016861:Cyclooxygenase Inhibitors; D014665:Vasodilator Agents; C067410:endothelium-dependent hyperpolarization factor; D019001:Nitric Oxide Synthase; D001920:Bradykinin