A sandwiched biological fluorescent probe for the diagnosis of human ovarian tumor based on TiO2 nanoparticles.
In this paper, we report a novel biological fluorescent probe for the diagnosis of human ovarian tumor based on sandwiched TiO(2) nanoparticles. The fluorescence nanoparticles consist of a fluorescent molecule, tetramethyl rhodamine isothiocyanate (TRITC), sandwiched between titanium dioxide (TiO(2)) nanoparticles and nano-gold via reacting with each other. The antibodies HER2, labeled on the surface of the biofluorescence nanoparticles, have granted nanoparticles the privilege of aiming at peculiar tumor antigen. The specificity of antibody-nanoparticles interacting with cells was characterized by Laser Scanning Confocal Microscope. The results showed that these sandwiched nanoparticles were innocuous and stable, and the method offered potential advantages of sensitivity and simplicity due to high combing efficiency between nanoparticles and cells and provided an alternative method for the diagnosis of human ovarian tumor (HOT).
Zhu
Peisi
P
College of Life and Environment, Shanghai Normal University, Shanghai, China.
Huang
Shasheng
S
Li
Mengyao
M
Ding
Na
N
Peng
Bing
B
Kong
Lingmi
L
Bo
Yang
Y
D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology; D014025:Titanium
D005456:Fluorescent Dyes; C009495:titanium dioxide; D014025:Titanium