title: Enhancement of thermal stabilization of formaldehyde dehydrogenase from Pseudomonas putida by directed evolution.
abstract: We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 degrees C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 degrees C), the variants obtained retained <80% activity for at least 500 h (21 d).
authors.affiliation: Toyota Central R&D Labs., Inc., Aichi-gun, Aichi.
authors.forename: Chie
authors.initials: C
authors.lastname: Imamura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shigemori
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019020:Directed Molecular Evolution; D004789:Enzyme Activation; D004795:Enzyme Stability; D016958:Pseudomonas putida / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent