Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244.
The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.
Vangnai
A S
AS
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. alisa.v@chula.ac.th
Promden
W
W
De-Eknamkul
W
W
Matsushita
K
K
Toyama
H
H
D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D020600:Gluconobacter oxydans / Q000145:classification / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012765:Shikimic Acid / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; C022511:quinate dehydrogenase