[Determination of human plasma concentration of asymmetric dimethylarginine by high performance liquid chromatography using an electrochemical detector].
Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of NO synthetase. Elevated ADMA levels are accompanied by impaired endothelium-dependent vasodilation in the brachial artery and increased intima-media thickness in the elastic arteries. The purpose of the study was to develop a procedure for qualitative and quantitative determination of the plasma content of free ADMA and symmetric dimethylarginine by reverse-phase high performance liquid chromatography in an isocratic elution mode, by applying an electrochemical detector. Ortho-phthalic aldehyde with the sulfur-containing component sodium sulfite was used as a derivation reagent. The mobile phase is a system that consists of acetonitrile, sodium hydrophosphate, and sodium dihydrophosphate. The retention time for the detectable substance on the column was 21.7 min. The total time of the analysis was 31 min. In patients with the clinical manifestations of atherosclerosis and in healthy individuals, the plasma ADMA concentration measured by the devised method was 0.69-0.30 and 0.13-0.04 microM, respectively.
Liadoshchuk
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Denisenko
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D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004563:Electrochemistry; D006801:Humans
C487735:dimethylarginine; D001120:Arginine