title: Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: evidence for opioid-immune crosstalk.
abstract: Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.
authors.affiliation: Department of Pharmacology and Physiology, Oklahoma State University-Center for Health Sciences, 1111 West 17(th) Street, Tulsa, OK 74107, USA.
authors.forename: Shekher
authors.initials: S
authors.lastname: Mohan
authors.forename: Randall L
authors.initials: RL
authors.lastname: Davis
authors.forename: Udaya
authors.initials: U
authors.lastname: DeSilva
authors.forename: Craig W
authors.initials: CW
authors.lastname: Stevens
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D009020:Morphine / Q000378:metabolism / Q000494:pharmacology*; D009447:Neuroblastoma / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D020239:Receptor Cross-Talk / Q000187:drug effects / Q000276:immunology*; D053573:Receptors, Interleukin-1 Type I / Q000037:antagonists & inhibitors / Q000502:physiology; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*
chemical_list: C508590:IL1R1 protein, human; D053583:Interleukin-1beta; D012333:RNA, Messenger; D053573:Receptors, Interleukin-1 Type I; D017450:Receptors, Opioid, mu; D009020:Morphine