Therapeutic antibodies: Discovery and development using the ProteOn XPR36 biosensor interaction array system.
Therapeutic monoclonal antibodies are becoming a significant and rapidly growing class of therapeutic pharmaceuticals. Their discovery and development requires fast and high-throughput methodologies for screening and selecting appropriate candidate antibodies having high affinity for the target as well as high specificity and low cross-reactivity. This study demonstrates the use of the ProteOn XPR36 protein interaction array system and its novel approach, termed One-Shot Kinetics, for the rapid screening and selection of high-affinity antibodies. This approach allows multiple quantitative protein binding analyses in parallel, providing association, dissociation, and affinity constants for several antibodies or supernatants simultaneously in one experiment. We show that the ProteOn XPR36 system is a valuable tool for use across multiple stages of the therapeutic antibody discovery and development process, enabling efficient and rapid screening after panning, affinity maturation, assay validation, and clone selection.
Bronner
Vered
V
Bio-Rad Laboratories, Gutwirth Park, Technion, Haifa 32000, Israel.
Denkberg
Galit
G
Peled
Mira
M
Elbaz
Yael
Y
Zahavi
Efrat
E
Kasoto
Harel
H
Reiter
Yoram
Y
Notcovich
Ariel
A
Bravman
Tsafrir
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D000915:Antibody Affinity / Q000276:immunology; D000941:Antigens / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D016466:CHO Cells; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D017452:Receptors, IgG / Q000276:immunology; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D017452:Receptors, IgG