Resistance of neonatal porcine Sertoli cells to human xenoantibody and complement-mediated lysis is associated with low expression of alpha-Gal and high production of clusterin and CD59.
BACKGROUND
Porcine Sertoli cells have an inherent resistance to human xenoreactive antibody and complement-mediated lysis, however, the mechanisms of protection are still unclear.


METHODS
Neonatal porcine Sertoli cells (NPSCs) were isolated from testes of 10 to 15-day-old piglets. Immortalized porcine aortic endothelial cells (iPECs) were used as control cells. An in vitro humoral injury model was used to assess whether NPSCs could resist human xenoantibody and complement-mediated lysis. Expressions of alpha-Gal, clusterin, CD59, CD46, and CD55 were examined to investigate the possible mechanisms of the immunoprotection of NPSCs.


RESULTS
Compared with iPECs, NPSCs significantly resisted human natural antibody and complement-mediated lysis when incubated with 20% normal human serum (NHS) in vitro (24.38 +/- 0.50 vs. 53.13 +/- 14.53%, P < 0.01). Mechanistically, NPSCs expressed lower level of alpha-Gal (Gmean: 41.78 +/- 2.39 vs. 95.17 +/- 2.39, P < 0.01), which was correlated with decreased binding of human xenoreactive IgG and IgM. Additionally, NPSCs expressed higher level of clusterin measured by both Western blot and fluorescence-activated cell sorter analysis and expressed more CD59 mRNA detected by reverse-transcription polymerase chain reaction.


CONCLUSIONS
These data demonstrate that the resistance of NPSCs to human xenoantibody and complement-mediated lysis is associated with low expression of xenoantigen alpha-Gal and high production of the complement inhibitors clusterin and CD59.
Yin
Zhuzeng
Z
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Wang
Lu
L
Xiang
Ying
Y
Ruan
Yongle
Y
Li
Junhua
J
Wang
Ximo
X
Ichim
Thomas E
TE
Chen
Shi
S
Chen
Gang
G
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology*; D018957:CD59 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D051152:Clusterin / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology*; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D008297:Male; D012708:Sertoli Cells / Q000276:immunology*; D013552:Swine; D014312:Trisaccharides / Q000276:immunology*
D000910:Antibodies, Heterophile; D018957:CD59 Antigens; D051152:Clusterin; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D003165:Complement System Proteins