title: Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.
abstract: We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.
authors.affiliation: Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Hokkaido, Japan.
authors.forename: Agathe
authors.initials: A
authors.lastname: Nkouawa
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
authors.forename: Tiaoying
authors.initials: T
authors.lastname: Li
authors.forename: Xingwang
authors.initials: X
authors.lastname: Chen
authors.forename: Toni
authors.initials: T
authors.lastname: Wandra
authors.forename: I Kadek
authors.initials: IK
authors.lastname: Swastika
authors.forename: Minoru
authors.initials: M
authors.lastname: Nakao
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yanagida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaya
authors.forename: Dongchuan
authors.initials: D
authors.lastname: Qiu
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D005243:Feces / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D013621:Taenia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology*