Metabolic alterations of DHEA and cholesterol sulphates in the hair of patients with acne measured by liquid chromatography-mass spectrometry.
As the hormonal levels in scalp hair reflects the condition of skin appendage, the level of dehydroepiandrosterone-3-sulphate (DHEAS) and cholesterol sulphate (CS) was evaluated in scalp hair obtained from patients with acne. The hair samples were extracted by alkaline solution and were analysed by liquid chromatography-mass spectrometry coupled to column switching system. The levels of DHEAS in scalp hair of women with acne were higher (P < 0.001) than controls, while the levels of CS in scalp hair of women and men with acne were higher (P < 0.001) than both control subjects. Increased hair levels of both DHEAS and CS could indicate acne development because of its direct action or stimulatory effect on local enzyme activity. It may be also helpful to understand the pathogenesis of acne based on follicular retention hyperkeratosis and increased sebum production of both steroid sulphates.
Cho
Sung-Hee
SH
Choi
Man Ho
MH
Sim
Woo Y
WY
Lee
Won-Yong
WY
Chung
Bong Chul
BC
D000152:Acne Vulgaris / Q000097:blood / Q000209:etiology / Q000378:metabolism*; D000328:Adult; D016022:Case-Control Studies; D002788:Cholesterol Esters / Q000378:metabolism*; D002853:Chromatography, Liquid; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood / Q000378:metabolism*; D005260:Female; D006197:Hair / Q000378:metabolism*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D012727:Sex Characteristics; D055815:Young Adult
D002788:Cholesterol Esters; D019314:Dehydroepiandrosterone Sulfate; C007045:cholesteryl sulfate