title: Short communication: ketone body concentration in milk determined by Fourier transform infrared spectroscopy: value for the detection of hyperketonemia in dairy cows.
abstract: The objective of this study was to evaluate Fourier transform infrared (FTIR) spectrometry to measure milk ketone bodies to detect hyperketonemic cows and compare this method with milk fat to protein ratio to detect hyperketonemia. Plasma and milk samples were obtained weekly from calving to wk 9 postpartum from 69 high-producing dairy cows. The reference test for hyperketonemia was defined as plasma concentration of beta-hydroxybutyrate (BHBA) >or=1,200 micromol/L. The weekly prevalence of hyperketonemia during the first 9 wk of lactation was, on average, 7.1%. Both BHBA and acetone in milk, determined by FTIR, had a higher sensitivity (80%) to detect hyperketonemia compared with milk fat to protein ratio (66%). Specificity was similar for the 3 diagnostic tests (71, 70, and 71%). In conclusion, FTIR predictions of BHBA or acetone in milk can detect cows with hyperketonemia in early lactation with a higher accuracy compared with the use of milk fat to protein ratio. Because of the high proportion of false-positive tests, there are concerns about the practical applicability of FTIR predictions of acetone, BHBA, and fat to protein ratio in milk to detect hyperketonemic cows.
authors.affiliation: Adaptation Physiology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands. Ariette.vanKnegsel@wur.nl
authors.forename: A T M
authors.initials: AT
authors.lastname: van Knegsel
authors.forename: S G A
authors.initials: SG
authors.lastname: van der Drift
authors.forename: M
authors.initials: M
authors.lastname: Horneman
authors.forename: A P W
authors.initials: AP
authors.lastname: de Roos
authors.forename: B
authors.initials: B
authors.lastname: Kemp
authors.forename: E A M
authors.initials: EA
authors.lastname: Graat
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003612:Dairying / Q000379:methods*; D005223:Fats / Q000032:analysis; D066298:In Vitro Techniques; D007659:Ketones / Q000032:analysis*; D007662:Ketosis / Q000175:diagnosis / Q000662:veterinary*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005223:Fats; D007659:Ketones; D008894:Milk Proteins