title: In vivo measurement of the human epidermal thickness in different localizations by multiphoton laser tomography.
abstract: BACKGROUND
  The in vivo measurement of epidermal thickness is still challenging. While ultrasound, optical coherence tomography and confocal laser microscopy are used with moderate success, this issue has not been addressed by multiphoton laser tomography.
  
  
  OBJECTIVE
  In the present study, an in vivo measurement of four different morphometric epidermal parameters is performed.
  
  
  METHODS
  Thirty healthy volunteers aged 21-82 years were included in the study after informed consent and approval of the local ethics committee. At the dorsal forearm and the dorsum of the hand, the thicknesses of the total epidermis, viable epidermis and stratum corneum and the depth of the papillary dermis were calculated from depth-resolved intensity curves after correlation with multiphoton images.
  
  
  RESULTS
  We have shown consistently that in all age groups, the four morphometric parameters are significantly higher at the hand compared with the forearm, while there were no differences between age groups. This is consistent with most previous findings.
  
  
  CONCLUSIONS
  The method presented here provides a novel in vivo investigation tool for the measurement of epidermal morphometric parameters that may be useful for the observation of epidermal changes over time in skin disorders, therapy side effects or in cosmetic science.
authors.affiliation: Department of Dermatology and Allergology, Friedrich Schiller University, Jena, Germany. johannes.koehler@med.uni-jena.de
authors.forename: Martin Johannes
authors.initials: MJ
authors.lastname: Koehler
authors.forename: Tanja
authors.initials: T
authors.lastname: Vogel
authors.forename: Peter
authors.initials: P
authors.lastname: Elsner
authors.forename: Karsten
authors.initials: K
authors.lastname: König
authors.forename: Rainer
authors.initials: R
authors.lastname: Bückle
authors.forename: Martin
authors.initials: M
authors.lastname: Kaatz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D046169:Dermoscopy / Q000295:instrumentation* / Q000379:methods; D004817:Epidermis / Q000033:anatomy & histology* / Q000473:pathology*; D005542:Forearm; D006225:Hand; D006801:Humans; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008875:Middle Aged; D015595:Skin Aging / Q000473:pathology*; D055815:Young Adult