title: mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.
abstract: The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) were studied in the mouse mammary gland from pregnancy to lactation. The relative mRNA abundance and percent change in real-time PCR were determined. mRNA expression of SLC27A3 and SLC27A4 was 37- and 1.4-fold more upregulated at 12 days of lactation, respectively (P < 0.01). Transcripts of SCD isoforms were the most abundant, accounting for 59% of all genes measured, and PPARGC1 isoforms were the least (0.06% of all genes measured). The mRNA abundance from ACC, FADS and LPIN accounted for 29, 9 and 2.6%, respectively. INSIG1 mRNA expression was 32-fold more upregulated (P < 0.05), while PPARGC1B was 0.18-fold downregulated at 18 days of lactation (P < 0.01). We concluded that mRNA abundance and expression of these isoforms are affected by the stage of lactation.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Han
authors.forename: H J
authors.initials: HJ
authors.lastname: Li
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wang
authors.forename: H S
authors.initials: HS
authors.lastname: Zhu
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Guo
authors.forename: W F
authors.initials: WF
authors.lastname: Lu
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
authors.forename: G Y
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D044943:Fatty Acid Desaturases / Q000235:genetics*; D050612:Fatty Acid Transport Proteins / Q000235:genetics*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010711:Phosphatidate Phosphatase; D011247:Pregnancy; D011270:Pregnancy, Animal; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D050612:Fatty Acid Transport Proteins; C506119:Insig1 protein, mouse; D008565:Membrane Proteins; D009687:Nuclear Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger; C493569:Slc27a1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C418978:Lpin1 protein, mouse; D010711:Phosphatidate Phosphatase; D000103:Acetyl-CoA Carboxylase