Upregulation of TLR2/4 expression in mononuclear cells in postoperative systemic inflammatory response syndrome after liver transplantation.
BACKGROUND
To explore the relationship between Toll-like rpheral blood mononuclear cells (PBMC) and systemic inflammatory response syndrome (SIRS) in postoperative patients of liver transplantation (LT).


METHODS
Blood samples of 27 patients receiving LT were collected at T1 (after induction of anaesthesia), T2 (25 minutes after the beginning of anhepatic phase), T3 (3 hours after graft reperfusion), and T4 (24 hours after graft reperfusion). The expression of TLR2/4 on PBMC and serum concentration of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-8 were measured. The patients were divided into SIRS group (n = 12) and non-SIRS group (n = 15) for analysis.


RESULTS
Blood loss and transfusion were higher in the SIRS group than in the non-SIRS group. Both the preanhepatic and anhepatic phase were significantly longer in the SIRS group. The TLR2/4 expression on PBMC as well as serum TNF-alpha, IL-1beta, and IL-8 were significantly higher at T3 and T4 than that at T1 and T2 in the SIRS patients. The expression of TLR4 on PBMC is positively correlated to serum TNF-alpha, IL-8. Expression of TLR2/4 on PBMC and serum concentrations of TNF-alpha, IL-1beta, did not differ among the 4-time points in non-SIRS patients.


CONCLUSIONS
Upregulation of TLR2/4 expression on PBMC may contribute to the development of postoperative SIRS during perioperative period of LT.
Hei
Ziqing
Z
Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. heiziqing0530@hotmail.com
Chi
Xinjin
X
Cheng
Nan
N
Luo
Gangjian
G
Li
Shangrong
S
D000328:Adult; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000276:immunology; D016209:Interleukin-8 / Q000097:blood / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D016031:Liver Transplantation / Q000009:adverse effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000209:etiology* / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
D053583:Interleukin-1beta; D016209:Interleukin-8; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha