[Anticancer effects of Pingyangmycin-activated carbon nanoparticles against human oral squamous carcinoma Tca8113 and BcaCD885 cell lines in vitro].
OBJECTIVE
The cytotoxic effects of a new formulation of Pingyangmycin-activated carbon nanoparticles (PYM-CH-NP) on two human oral squamous cell carcinoma Tca8113 and BcaCD885 cell lines were studied in vitro.


METHODS
The inhibitory effects of PYM-CH-NP and Pingyangmycin (PYM) were evaluated by methyl thiazolyl tetrazolium (MTT) assay at 1-7 days. The 50% inhibition concentration values (IC50) and relative antitumor activity (RAA) of PYM-CH-NP and PYM against Tca8113 and BcaCD885 with different drug concentration were evaluated. The time-dependent cytotoxic effects of PYM-CH-NP and PYM were during 1-5 days, so the doseeffect relationship was investigated at 5th day.


RESULTS
Both PYM-CH-NP and PYM had high anticancer effects on Tca8113 and BcaCD885, and the cytotoxic effects were dose-dependent and time-dependent.


CONCLUSIONS
The activated carbon nanoparticles (CH-NP) may serve as a new drug delivery carrier of PYM. The new formulation PYM-CH-NP could slow down drug release, prolonged the drug concentration and its acting time, so more effective anticancer efficacy could be achieved.
Sun
Ming-Lei
ML
Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Wang
Chang-Mei
CM
Wen
Yu-Ming
YM
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin / Q000031:analogs & derivatives; D002244:Carbon; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D066298:In Vitro Techniques; D009062:Mouth Neoplasms; D053758:Nanoparticles
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C025703:bleomycetin; D002244:Carbon