Transcriptome analysis of the phytobacterium Xylella fastidiosa growing under xylem-based chemical conditions.
Xylella fastidiosa is a xylem-limited bacterium responsible for important plant diseases, like citrus-variegated chlorosis (CVC) and grapevine Pierce's disease (PD). Interestingly, in vitro growth of X. fastidiosa in chemically defined media that resemble xylem fluid has been achieved, allowing studies of metabolic processes used by xylem-dwelling bacteria to thrive in such nutrient-poor conditions. Thus, we performed microarray hybridizations to compare transcriptomes of X. fastidiosa cells grown in 3G10-R, a medium that resembles grape sap, and in Periwinkle Wilt (PW), the complex medium traditionally used to cultivate X. fastidiosa. We identified 299 transcripts modulated in response to growth in these media. Some 3G10R-overexpressed genes have been shown to be upregulated in cells directly isolated from infected plants and may be involved in plant colonization, virulence and environmental competition. In contrast, cells cultivated in PW show a metabolic switch associated with increased aerobic respiration and enhanced bacterial growth rates.
Ciraulo
Maristela Boaceff
MB
Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Avenida Dr. Cândido Xavier de Almeida Souza 200, Mogi das Cruzes SP, Brazil.
Santos
Daiene Souza
DS
Rodrigues
Ana Claudia de Freitas Oliveira
AC
de Oliveira
Marcus Vinícius
MV
Rodrigues
Tiago
T
de Oliveira
Regina Costa
RC
Nunes
Luiz R
LR
D000332:Aerobiosis / Q000187:drug effects / Q000235:genetics; D003470:Culture Media / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D044167:Xylella / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D052584:Xylem / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*
D003470:Culture Media