title: Saturnispora serradocipensis sp. nov. and Saturnispora gosingensis sp. nov., two ascomycetous yeasts from ephemeral habitats.
abstract: Two novel ascomycetous yeast species, Saturnispora serradocipensis and Saturnispora gosingensis, were isolated from leaf detritus in a tropical stream of Southeastern Brazil and a mushroom collected in Taiwan, respectively. Analysis of the D1/D2 domains of the large-subunit of the rRNA gene of these strains showed that these species are related to Saturnispora hagleri, their closest relative. Saturnispora serradocipensis and S. gosingensis differed from S. hagleri, respectively, by seven nucleotide substitutions and two indels and three nucleotide substitutions and three indels in D1/D2 rRNA sequences. The two new species differ from each another by four nucleotide substitutions and one indel in D1/D2 rRNA sequences. However, the ITS sequences of S. serradocipensis, S. gosingensis and S. hagleri were quite divergent, showing that they are genetically separate species. The type strain of S. serradocipensis is UFMG-DC-198(T) (=CBS 11756(T) = NRRL Y-48717(T)), and of S. gosingensis GA4M05(T) is (CBS 11755(T) = NRRL Y-48718(T)).
authors.affiliation: Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Monica R
authors.initials: MR
authors.lastname: Canelhas
authors.forename: Anne C
authors.initials: AC
authors.lastname: Barbosa
authors.forename: Adriana O
authors.initials: AO
authors.lastname: Medeiros
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.forename: Li-Yin
authors.initials: LY
authors.lastname: Huang
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
mesh_terms: D001938:Brazil; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S