The correlations among serum tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and sialic acids with peripheral lymphocytes in bovine tropical theileriosis.
The infection with protozoan parasite Theileria annulata induces changes triggering the activation and/or proliferation of the host lymphocytes. In order to find out the possible correlations among peripheral circulatory lymphocytes, cytokine activities and the level of sialic acids, 50 dairy Holstein cattle, naturally infected with T. annulata, were divided into 4 subgroups according to their parasitemia rates (<1%, 1-3%, 3-5% and >5%). Also, ten non-infected cattle were sampled as control group. Blood samples were taken from jugular vein into acid citrate dextrose-containing tubes for measuring hematological parameters and B and T (CD(4) and CD(8)) cell populations and without anticoagulant for TNF-alpha, IFN-gamma and sialic acid concentrations. Remarkable decreases observed in red blood cells (RBCs), white blood cells (WBCs) and packed cell volume (PCV) in infected cattle compared to healthy ones (P < 0.05). Also, with increase in parasitemia rate, total lymphocytes and monocytes alleviated in the diseased groups. By contrast, total neutrohpils and the concentrations of TNF-alpha, IFN-gamma and total sialic acids were significantly elevated (P < 0.05) in infected animals. Accordingly, the circulatory populations of CD(4) and CD(8) T cells and B cells showed a substantial decrease, while a significant increase was observed in T (CD(4) and CD(8)) cells in cattle infected with <1% parasitemia rates. Decreased circulatory T cell population shows the ineffective responses of T cells to the stimulatory cytokines such as IFN-gamma or TNF-alpha. On the other hand, the elevation of cytokines (particularly IFN-gamma) and sialic acids have presumably an inhibitory role on circulatory B cell population in infected cattle. In addition, a high level of sialic acid concentration indicates the probable role of sialic acid to regulate the parasite-host cell adhesion during sporozoites invasion.
Razavi
Seyed Mostafa
SM
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz, 71345, Iran. mrazavi@shirazu.ac.ir
Nazifi
Saeed
S
Emadi
Mahboobeh
M
Rakhshandehroo
Ehsan
E
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000469:parasitology; D002417:Cattle; D003612:Dairying; D005260:Female; D007371:Interferon-gamma / Q000097:blood*; D012794:Sialic Acids / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism* / Q000469:parasitology; D013801:Theileriasis / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D012794:Sialic Acids; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma