title: [Decreased circulating endothelial progenitor cell function: relationship with serum concentrations of high-sensitivity C-reactive protein in children with Kawasaki disease].
abstract: OBJECTIVE
  To study the function of circulating endothelial progenitor cells and its relationship with serum concentrations of high-sensitivity C-reactive protein (Hs-CRP) in children with Kawasaki disease.
  
  
  METHODS
  Ten children with Kawasaki disease and ten healthy children as a control group were enrolled. The peripheral mononuclear cells were induced into endothelial progenitor cells using Dulbecco's Modified Eagle Medium containing vascular endothelial growth factor and basic fibroblast growth factor. The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells were assessed by MTT methods, modified Boyden chamber methods and cell culture plate adhesion method, respectively. The concentrations of serum Hs-CRP were measured by latex enhanced turbidimetric immunoassay.
  
  
  RESULTS
  The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells in the Kawasaki disease group were significantly lower than those in the control group (P<0.01). The serum concentrations of Hs-CRP in the Kawasaki disease group were significantly higher than those in the control group (87.1+/-30.2 mg/L vs 5.3+/-3.4 mg/L; P<0.01). The function of circulating endothelial progenitor cells was negatively correlated with serum concentrations of Hs-CRP in the Kawasaki disease group.
  
  
  CONCLUSIONS
  The function of circulating endothelial progenitor cells is decreased in children with Kawasaki disease, which may be associated with the abnormal expression of inflammatory mediators.
authors.affiliation: Cardiovascular Center, Shenzhen Children's Hospital, Shenzhen, Guangdong 518026, China. nihaomingguo@yahoo.com.cn
authors.forename: Ming-Guo
authors.initials: MG
authors.lastname: Xu
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Men
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zu
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Hua-Bo
authors.initials: HB
authors.lastname: Cai
authors.forename: Xiang-Chun
authors.initials: XC
authors.lastname: Meng
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis*; D002675:Child, Preschool; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009080:Mucocutaneous Lymph Node Syndrome / Q000097:blood*; D013234:Stem Cells / Q000502:physiology*
chemical_list: D002097:C-Reactive Protein