Effect of preserving condition of porcine ovaries on the development of in vitro matured oocytes.
The effect of preservation condition of ovaries on the in vitro maturation of the porcine oocytes was studied. Cumulus-oocyte complexes (COCs) were obtained from the ovaries preserved in Dulbecco's phosphate buffered saline (PBS) solution at various temperatures for different time intervals, and cultured in M199 maturation medium. Matured oocytes were obtained from the ovaries preserved in PBS for 8 h and electrically activated. The activated oocytes were then cultured in NCSU23 embryo culture medium for 16 h to observe activation or 144 h to observe embryo development. It was found that the preservation temperature affected the maturation of porcine oocytes greatly. The effect was described as a compromise of the suppressions of autolysis at physiological temperature and frostbite because of low temperature. A preservation temperature of approximately 25°C showed the maximum maturation rate for a preservation time of 8 h. Preservation temperature also affected the activation and embryo development of porcine oocytes greatly, following a trend similar to the effect of preservation temperature on the maturation. Based on maturation rate, activation rate and cleavage rate, a preservation temperature of approximately 25°C would be optimum for a preservation time of 8 h.
Lin
Y-A
YA
Department of Tropical Agriculture and International Cooperation, National Pingtung, Taiwan.
Tsai
H-B
HB
Liao
M-H
MH
Chen
M-C
MC
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary*; D005260:Female; D009865:Oocytes / Q000502:physiology*; D010053:Ovary / Q000502:physiology*; D013552:Swine / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods / Q000662:veterinary*