Imaging targeted-agent binding in vivo with two probes.
An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3 x 10(-5) s(-1)), as compared to the normal pancreas (3.4 x 10(-5) s(-1)). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery.
Pogue
Brian W
BW
Samkoe
Kimberley S
KS
Hextrum
Shannon
S
O'Hara
Julia A
JA
Jermyn
Michael
M
Srinivasan
Subhadra
S
Hasan
Tayyaba
T
D000818:Animals; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007700:Kinetics; D016014:Linear Models; D051379:Mice; D008954:Models, Biological; D010190:Pancreatic Neoplasms / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014183:Transplantation, Heterologous; D051598:Whole Body Imaging
D005456:Fluorescent Dyes; D004815:Epidermal Growth Factor