The structure of the carbohydrate backbone of the lipooligosaccharide from an alkaliphilic Halomonas sp.
The structure of the carbohydrate backbone of the core-lipid A region of the lipooligosaccharide (LOS) of the alkaliphilic and slightly halophilic bacterium Halomonas sp. has been elucidated. The LOS was fully deacylated, dephosphorylated, and reduced at the free reducing end. The structure, obtained by means of compositional analysis, 2D NMR spectroscopy, and MALDI mass spectrometry, was determined as the following: [ structure: see text ]. All sugars are d-pyranoses; Hep is l-glycero-d-manno-heptose and Kdo is 3-deoxy-d-manno-oct-2-ulosonic acid.
Silipo
Alba
A
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy.
Gargiulo
Valentina
V
Sturiale
Luisa
L
Marchetti
Roberta
R
Prizeman
Paul
P
Grant
William D
WD
De Castro
Cristina
C
Garozzo
Domenico
D
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
Molinaro
Antonio
A
D002240:Carbohydrate Sequence; D020632:Halomonas / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides