mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle.
The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) were studied in the mouse mammary gland from pregnancy to lactation. The relative mRNA abundance and percent change in real-time PCR were determined. mRNA expression of SLC27A3 and SLC27A4 was 37- and 1.4-fold more upregulated at 12 days of lactation, respectively (P < 0.01). Transcripts of SCD isoforms were the most abundant, accounting for 59% of all genes measured, and PPARGC1 isoforms were the least (0.06% of all genes measured). The mRNA abundance from ACC, FADS and LPIN accounted for 29, 9 and 2.6%, respectively. INSIG1 mRNA expression was 32-fold more upregulated (P < 0.05), while PPARGC1B was 0.18-fold downregulated at 18 days of lactation (P < 0.01). We concluded that mRNA abundance and expression of these isoforms are affected by the stage of lactation.
Han
L Q
LQ
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Li
H J
HJ
Wang
Y Y
YY
Zhu
H S
HS
Wang
L F
LF
Guo
Y J
YJ
Lu
W F
WF
Wang
Y L
YL
Yang
G Y
GY
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D044943:Fatty Acid Desaturases / Q000235:genetics*; D050612:Fatty Acid Transport Proteins / Q000235:genetics*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010711:Phosphatidate Phosphatase; D011247:Pregnancy; D011270:Pregnancy, Animal; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors
D050612:Fatty Acid Transport Proteins; C506119:Insig1 protein, mouse; D008565:Membrane Proteins; D009687:Nuclear Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D020033:Protein Isoforms; D012333:RNA, Messenger; C493569:Slc27a1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; C418978:Lpin1 protein, mouse; D010711:Phosphatidate Phosphatase; D000103:Acetyl-CoA Carboxylase