[Cardioprotective effects of St. Thomas solution containing PolyHb on isolated rat heart during cold preservation].
OBJECTIVE
To investigate the cardioprotective effects of St. Thomas solution containing PolyHb on ischemia-reperfusion injury to isolated rat heart after eight-hour cold storage.


METHODS
Sixty male inbred SD rats were randomly divided into 3 groups (n = 20 in each group): sham group, St. Thomas solution (STS) group and STS with PolyHb group. The Langendoff isolated rat heart model was used. The hearts were stored in 4 degrees C St. Thomas solution with or without PolyHb for 8 h followed by 120 min of reperfusion. The recovery of cardiac function was observed. Myocardial enzyme leakage and infarct size were measured after reperfusion.


RESULTS
In comparison with STS group, St. Thomas solution containing PolyHb improved the recovery of LVDP (P < 0.001) and +/- dp/dt (P < 0.001), decreased the release of CK and LDH (P < 0.001), reduced the percentage of the infarct size (P < 0.001) of the heart during reperfusion as well.


CONCLUSIONS
Adding PolyHb to St. Thomas solution can protect myocardium from ischemia-reperfusion injury after eight-hour cold preservation.
Zhang
Pu
P
Department of Pharmacology & Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Li
Tao
T
Liu
Jin
J
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D006321:Heart / Q000503:physiopathology*; D006324:Heart Arrest, Induced; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology
D001639:Bicarbonates; D002316:Cardiotonic Agents; D006454:Hemoglobins; D011108:Polymers; C041711:St. Thomas' Hospital cardioplegic solution; C080663:polymerized bovine hemoglobin; D012965:Sodium Chloride; D011189:Potassium Chloride; D008274:Magnesium; D002122:Calcium Chloride