Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate.
The aim of this study was to evaluate the effect of different concentrations of triethylene glycol dimethacrylate (TEGDMA) on the inhibition of matrix metalloproteinase 2 (MMP-2). Mouse gingival explants were cultured overnight in DMEM and the expression of secreted enzymes was analyzed by gelatin zymography in buffers containing 5 mM CaCl(2) (Tris-CaCl(2)) in 50 mM Tris-HCl buffer with the addition of TEGDMA at different concentrations (0.62%, 1.25%, 2.5%, or 5.0% (v/v)). The gelatinolytic proteinase present in the conditioned media was characterized as matrix metalloproteinase by means of specific chemical inhibition. The matrix metalloproteinases present in the conditioned media were characterized as MMP-2 by immunoprecipitation. The eletrophoretic bands were scanned and the transmittance values were analyzed. Data was plotted and submitted to linear regression to investigate MMP-2 inhibition as a function of TEGDMA concentration. Three major bands were detected in the zymographic assays. These bands were characterized as MMP-2. Zymogene (72 kDa), intermediate (66 kDa) and active forms of MMP-2 (62 kDa) were inhibited by TEGDMA in a dose-dependent way. These findings suggest that TEGDMA could inhibit MMP-2 expression even at small concentrations.
de Carvalho
Rodrigo Varella
RV
Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves, 457, Pelotas, RS, CEP: 96015-560, Brazil. rodrigo.varella@gmail.com
Ogliari
Fabrício Aulo
FA
Marques
Marcelo Rocha
MR
de Souza
Ana Paula
AP
Petzhold
Cesar Liberato
CL
Line
Sergio Roberto Peres
SR
Piva
Evandro
E
Etges
Adriana
A
D000818:Animals; D003188:Composite Resins / Q000008:administration & dosage / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D003764:Dental Materials / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005881:Gingiva / Q000201:enzymology*; D047468:Immunoprecipitation; D007202:Indicators and Reagents; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D012394:Rosaniline Dyes; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques
D003188:Composite Resins; D017077:Culture Media, Conditioned; D003764:Dental Materials; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D061965:Matrix Metalloproteinase Inhibitors; D011109:Polymethacrylic Acids; D012394:Rosaniline Dyes; D015842:Serine Proteinase Inhibitors; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D004492:Edetic Acid; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; C004692:coomassie Brilliant Blue; D005033:Ethylmaleimide