title: Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions.
abstract: Chromatography technology was employed to clarify the mechanism of interaction between multi-wall carbon nanotubes (MWCNT) and proteins. A column (16x100 mm) was packed with purified MWCNT, and various proteins were eluted with phosphate buffered saline (PBS) with and without gradient systems. It was found that albumin in bovine serum was eluted immediately from the column without any adsorption to MWCNT. Conversely, the non-albumin proteins, including a protein of 85 kDa molecular mass and a group of proteins with molecular masses higher than 115 kDa, exhibited considerably high affinity towards MWCNT. A sample of pure bovine serum albumin was also eluted immediately from the column, while lysozyme did not elute as a peak with PBS, but eluted with 0.6 M NaCl. Fundamentally, carbon nanotubes are devoid of any electrical charge. Therefore, other forces including the hydrogen bonds, hydrophilic interactions, and van der Waals forces were most probably responsible for the differential elution behaviors. In conclusion, this chromatographic method provided a simple and direct analysis of the interactions between carbon nanotubes and the various proteins.
authors.affiliation: Hokkaido University, Kita-ku, Sapporo, Japan. kuboki.yoshinori@gmail.com
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kuboki
authors.forename: Takamitu
authors.initials: T
authors.lastname: Koshikawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takita
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Fujisawa
authors.forename: Min-ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Abe
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Akasaka
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.forename: Fumio
authors.initials: F
authors.lastname: Watari
authors.forename: Rachel
authors.initials: R
authors.lastname: Sammons
mesh_terms: D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D002417:Cattle; D055598:Chemical Phenomena; D002845:Chromatography / Q000295:instrumentation*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006860:Hydrogen Bonding; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010710:Phosphates; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D012965:Sodium Chloride; D017155:Wettability
chemical_list: D001798:Blood Proteins; D002021:Buffers; D037742:Nanotubes, Carbon; D010710:Phosphates; D012709:Serum Albumin; D012965:Sodium Chloride; D009113:Muramidase