An in vitro study on reproductive toxicology of Deltamethrin on rat spermatozoa.
Recent findings indicate that synthetic pyrethroid insecticide may induce toxic manifestations by enhancing the production of reactive oxygen species and disrupting the balance between pro-oxidants and antioxidants as a result of lipid peroxidation (LP) of cell membranes. The aim of the study was to examine the potency of Deltamethrin (Del) to induce oxidative stress response in rat spermatozoa in vitro. Spermatozoa were incubated with different concentrations (0, 10, 50, 100 and 200 microm) of Del for 3 h at 37 degrees C. After that, sperm parameters (motility, viability and abnormal morphology), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) levels were determined. We found that in vitro exposure to Del caused a significant decline of sperm motility and viability and increase of abnormal sperm morphology, MDA, SOD and CAT levels at different concentrations of Del. This study demonstrated that Del caused deterioration in sperm motility and viability, and induction in LP, abnormal morphology of spermatozoa and antioxidants enzyme activities.
Abdallah
F Ben
FB
Human Pathologies and Oxidative Stress Unit, Superior Institute of Biotechnology of Sfax, Sfax, Tunisia. fatma_isbs@yahoo.fr
Hamden
K
K
Galeraud-Denis
I
I
El Feki
A
A
Keskes-Ammar
L
L
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009570:Nitriles / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D017382:Reactive Oxygen Species; C017180:decamethrin; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase