title: [Effect of Matrine on cell cycle and human telomerase reverse transcriptase of human salivary adenoid cystic carcinoma].
abstract: OBJECTIVE
  To investigate the effect of the traditional Chinese medicine Matrine on cell cycle and human telomerase reverse transcriptase (hTERT) of human ACC-M cell lines.
  
  
  METHODS
  Different concentrations of Matrine were used in the medium of ACC-M cells. Change of cell cycle were detected by flow cytometry after ACC-M cell were cultivated with different concentrations Matrine (0.25, 0.50, 0.75, 1.00 mg x mL(-1)). Expression of hTERT was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescene and flow cytometry quantitative analysis.
  
  
  RESULTS
  Matrine caused obviously the GdG1 phase block and inhibited proliferation of ACC-M cells. At same time, this effect was positive correlation to Matrine concentration and treat time. Matrine can inhibit the expression of hTERT mRNA and protein.
  
  
  CONCLUSIONS
  Matrine can obviously inhibit cell cycle and down-regulate expression of hTERT. Inhibition of cell cycle is possible correlation with down-regulation expression of hTERT.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Zhao
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Xie
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Li
authors.forename: Wan-Tao
authors.initials: WT
authors.lastname: Chen
authors.forename: Ming-Lei
authors.initials: ML
authors.lastname: Sun
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
mesh_terms: D000470:Alkaloids; D003528:Carcinoma, Adenoid Cystic; D002453:Cell Cycle; D006801:Humans; D011807:Quinolizines; D012333:RNA, Messenger; D019098:Telomerase; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D012333:RNA, Messenger; C509186:TERT protein, human; D019098:Telomerase; D000093842:Matrines