[Permeability of exogenetic recombine human erythropoietin through placenta barrier to fetal rats with transient uteroplacental ischemia].
OBJECTIVE
To investigate whether recombine human erythropoietin can cross the placenta barrier in rats with transient uteroplacental ischemia.


METHODS
Rats on day 19 of pregnancy were divided into ischemia-reperfusion group, sham-operated group and rhEPO treated group. Fetal ischemia was induced by bilateral occlusion of the utero-ovarian artery for 20 minutes. 125I-rhEPO or saline were administered intravenously 30 min before the induction of hypoxic-ischemic injury. Fetal rat organs were removed to measure the radioactivity post injury.


RESULTS
A small amount of radioactive activity (1.26 +/- 0.28) pg/g was detected in the fetal rats with ischemia-reperfusion. 125I-rhEPO radioactivity increased gradually with time in the placenta, amniotic fluid and fetal tissues of vital organs in the rats of sham-operated group and rhEPO treatment group. There were significant differences in 125I-rhEPO between placental organs and other organs (P < 0.05). The permeability of 125I-rhEPO through the blood brain barrier changed with reperfusion time and peaked about 6 h after reperfusion, and significant differences were found between rhEPO treatment group and sham operation group (P < 0.05).


CONCLUSIONS
Exogenous rhEPO can cross the placenta barrier and blood-brain barrier and reach hypoxic-ischemic fetal rats.
Zhou
Jun
J
Department of Anesthesia, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Ma
Yu-Shan
YS
Liu
Hui
H
Lin
Xue-Mei
XM
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects; D004921:Erythropoietin / Q000493:pharmacokinetics*; D005260:Female; D005311:Fetal Hypoxia / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D008431:Maternal-Fetal Exchange / Q000187:drug effects; D010920:Placenta / Q000098:blood supply* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011994:Recombinant Proteins; D015427:Reperfusion Injury / Q000378:metabolism*; D014599:Uterus / Q000098:blood supply
D011994:Recombinant Proteins; D004921:Erythropoietin