Real-time fluorescent quantitative immuno-PCR method for determination of fluoranthene in water samples with a molecular beacon.
A reliable and sensitive competitive real-time fluorescent quantitative immuno-PCR (RTFQ-IPCR) assay using a molecular beacon was developed for the determination of trace fluoranthene (FL) in the environment. Under optimized assay conditions, FL can be determined in the concentration range from 1 fg/mL to 100 ng/mL, withy = 0.194x + 7.859, and a correlation coefficient of 0.967 was identified, with a detection limit of 0.6 fg/mL. Environmental water samples were successfully analyzed, recovery was between 90% and 116%, with intra-day relative standard deviation (RSD) of 6.7%-12.8% and inter-day RSD of 8.4%-15.2%. The results obtained from RTFQ-IPCR were confirmed by ELISA, showing good accuracy and suitability to analyze FL in field samples. As a highly sensitive method, the molecular beacon-based RTFQ-IPCR is acceptable and promising for providing reliable test results to make environmental decisions.
Ye
Qiyan
Q
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China. yeqy76@163.com
Zhuang
Huisheng
H
Zhou
Chun
C
Wang
Qiong'e
Q
D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005449:Fluorenes / Q000032:analysis*; D005453:Fluorescence; D015335:Molecular Probes / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D005449:Fluorenes; D015335:Molecular Probes; D014867:Water; C007738:fluoranthene