Expression of dopamine pathway genes in the midbrain is independent of known ETS transcription factor activity.
In nematodes, the ETS-family transcription factor ast-1 regulates multiple genes comprising the dopamine (DA) neuron phenotype, including biosynthetic enzymes and transporters. ETS transcription factors are hypothesized to play a similar role in vertebrates, and based on its expression in the adult mouse midbrain, Etv5/ERM has been proposed as a regulator of DA gene expression in the substantia nigra (SN) and ventral tegmental area (VTA). Here we show that Etv5 expression is not detectable until postnatal stages in the midbrain, well after development of the DA system, and that Etv5 knock-out and control mice show comparable tyrosine hydroxylase and dopamine transporter expression in the embryonic and adult midbrain. Other known members of the ETS family do not have expression patterns that are consistent with a role in DA gene regulation in the SN/VTA. These findings suggest that the ETS factors, while required for the generation of the DA phenotype in nematodes, do not play such a role in the mouse midbrain.
Wang
Shirong
S
Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington 98101, USA.
Turner
Eric E
EE
D000818:Animals; D000831:Animals, Newborn; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D004298:Dopamine / Q000235:genetics / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008636:Mesencephalon / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000172:deficiency / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism; D050493:Vesicular Monoamine Transport Proteins / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; C513242:Etv5 protein, mouse; D012333:RNA, Messenger; C493454:Slc18a2 protein, rat; C493216:Slc6a3 protein, rat; D014157:Transcription Factors; D050493:Vesicular Monoamine Transport Proteins; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine