Laboratory-scale protein striping system for patterning biomolecules onto paper-based immunochromatographic test strips.
A method for patterning narrow lines of biomolecules onto nitrocellulose membranes using laboratory syringe pumps is described. One syringe pump is used to drive the biomolecule solution through a needle, while another modified syringe pump acts as a one-dimensional translation stage, moving the needle across the membrane much like a pen. This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips.
Nash
Michael A
MA
University of Washington, Department of Bioengineering, Box 355061, 3720 15th Ave NE, Seattle, WA 98195-5061, USA. mikenash@uw.edu
Hoffman
John M
JM
Stevens
Dean Y
DY
Hoffman
Allan S
AS
Stayton
Patrick S
PS
Yager
Paul
P
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D019411:Clinical Laboratory Techniques; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D010209:Paper; D011506:Proteins / Q000737:chemistry* / Q000276:immunology; D011934:Reagent Strips; D019809:Streptavidin / Q000737:chemistry / Q000276:immunology; D013594:Syringes; D013997:Time Factors
D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D011506:Proteins; D011934:Reagent Strips; D006046:Gold; D019809:Streptavidin