title: The structure of the carbohydrate backbone of the lipooligosaccharide from an alkaliphilic Halomonas sp.
abstract: The structure of the carbohydrate backbone of the core-lipid A region of the lipooligosaccharide (LOS) of the alkaliphilic and slightly halophilic bacterium Halomonas sp. has been elucidated. The LOS was fully deacylated, dephosphorylated, and reduced at the free reducing end. The structure, obtained by means of compositional analysis, 2D NMR spectroscopy, and MALDI mass spectrometry, was determined as the following: [ structure: see text ]. All sugars are d-pyranoses; Hep is l-glycero-d-manno-heptose and Kdo is 3-deoxy-d-manno-oct-2-ulosonic acid.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Valentina
authors.initials: V
authors.lastname: Gargiulo
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Roberta
authors.initials: R
authors.lastname: Marchetti
authors.forename: Paul
authors.initials: P
authors.lastname: Prizeman
authors.forename: William D
authors.initials: WD
authors.lastname: Grant
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D002240:Carbohydrate Sequence; D020632:Halomonas / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
chemical_list: D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides