title: Cross immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus (Boophilus) microplus infestation.
abstract: Recombinant Glutathione S-transferase of Haemaphysalis longicornis (rGST-Hl) was expressed in Escherichia coli, purified by affinity chromatography and used in the immunization of cattle. Western blot analysis showed positive antibody response in cattle immunized with rGST-Hl. The tests also showed that immunized bovine sera recognize native Rhipicephalus microplus proteins in different tissue extracts. Furthermore, the vaccine potential of rGST-Hl was investigated against infestation of Hereford cattle by R. microplus. Vaccination of cattle with rGST-Hl conferred partial cross-protection immunity against R. microplus. Considering the effect on number of engorged ticks, egg laying capacity and egg fertility, the overall efficacy of vaccination was of 57%, as compared with control group.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500 Prédio 43421, Porto Alegre, RS 91501-970, Brazil.
authors.forename: Luís Fernando
authors.initials: LF
authors.lastname: Parizi
authors.forename: Kiyoko Uemura
authors.initials: KU
authors.lastname: Utiumi
authors.forename: Saiki
authors.initials: S
authors.lastname: Imamura
authors.forename: Misao
authors.initials: M
authors.lastname: Onuma
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
authors.forename: Itabajara
authors.initials: I
authors.lastname: da Silva Vaz
mesh_terms: D000465:Algorithms; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D026863:Ixodidae / Q000201:enzymology / Q000276:immunology*; D048494:Rhipicephalus / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D007106:Immune Sera; D014614:Vaccines, Synthetic; D005982:Glutathione Transferase