Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples.
The aim of this study was to compare the detection of Ehrlichia canis morulae and DNA by nPCR in whole blood and spleen aspiration. The sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears. E. canis DNA was detected in 29/40 blood samples. We verified that morulae detection is more efficient in cytological preparations from spleen aspiration. On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis.
Faria
Joice Lara Maia
JL
Medicina Veterinária, Universidade de Uberaba – UNI. mirelatc@fcav.unesp.br
Dagnone
Ana Sílvia
AS
Munhoz
Thiago Demarchi
TD
João
Carolina Franchi
CF
Pereira
Wanderson Adriano Biscola
WA
Machado
Rosângela Zacarias
RZ
Tinucci-Costa
Mirela
M
D000818:Animals; D004269:DNA, Bacterial / Q000097:blood*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000469:parasitology*; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics*; D016873:Ehrlichiosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000382:microbiology*; D013396:Suction
D004269:DNA, Bacterial