title: Binding of von Willebrand factor to collagen and glycoprotein Ibalpha, but not to glycoprotein IIb/IIIa, contributes to ischemic stroke in mice--brief report.
abstract: OBJECTIVE
  To unravel crucial von Willebrand factor (VWF) interactions that are detrimental in stroke development.
  
  
  RESULTS
  VWF(-/-) mice received gene transfer to express mutants of VWF defective either in binding to fibrillar collagen, glycoprotein (GP)Ibα or GPIIb/IIIa, and underwent 60 minutes of transient middle cerebral artery occlusion. In VWF(-/-) mice reconstituted with VWF mutants defective in binding to collagen or GPIbα, protection against stroke was sustained, whereas VWF lacking the GPIIb/IIIa binding site restored full susceptibility similar to normal VWF.
  
  
  CONCLUSIONS
  VWF-collagen and VWF-GPIbα (but not VWF-GPIIb/IIIa) interactions are instrumental in thrombus formation after transient middle cerebral artery occlusion, and their inhibition could be a promising target for stroke treatment.
authors.affiliation: Laboratory for Thrombosis Research, KULeuven Campus Kortrijk, E Sabbelaan 53, B-8500 Kortrijk, Belgium. simon.demeyer@kuleuven-kortrijk.be
authors.forename: Simon F
authors.initials: SF
authors.lastname: De Meyer
authors.forename: Tobias
authors.initials: T
authors.lastname: Schwarz
authors.forename: Hans
authors.initials: H
authors.lastname: Deckmyn
authors.forename: Cécile V
authors.initials: CV
authors.lastname: Denis
authors.forename: Bernhard
authors.initials: B
authors.lastname: Nieswandt
authors.forename: Guido
authors.initials: G
authors.lastname: Stoll
authors.forename: Karen
authors.initials: K
authors.lastname: Vanhoorelbeke
authors.forename: Christoph
authors.initials: C
authors.lastname: Kleinschnitz
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000209:etiology* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020521:Stroke / Q000209:etiology* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; D011994:Recombinant Proteins; D014841:von Willebrand Factor; D003094:Collagen