A genomic diagnostic tool for human endometrial receptivity based on the transcriptomic signature.
OBJECTIVE
To create a genomic tool composed of a customized microarray and a bioinformatic predictor for endometrial dating and to detect pathologies of endometrial origin. To define the transcriptomic signature of human endometrial receptivity.


METHODS
Two cohorts of endometrial samples along the menstrual cycle were used: one to select the genes to be included in the customized microarray (endometrial receptivity array [ERA]), and the other to be analyzed by ERA to train the predictor for endometrial dating and to define the transcriptomic signature. A third cohort including pathological endometrial samples was used to train the predictor for pathological classification.


METHODS
Healthy oocyte donors and patients.


METHODS
Healthy fertile women (88) and women with implantation failure (5) or hydrosalpinx (2).


METHODS
Human endometrial biopsies.


METHODS
The gene expression of endometrial biopsies.


RESULTS
The ERA included 238 selected genes. The transcriptomic signature was defined by 134 genes. The predictor showed a specificity of 0.8857 and sensitivity of 0.99758 for endometrial dating, and a specificity of 0.1571 and a sensitivity of 0.995 for the pathological classification.


CONCLUSIONS
This diagnostic tool can be used clinically in reproductive medicine and gynecology. The transcriptomic signature is a potential endometrial receptivity biomarkers cluster.
Díaz-Gimeno
Patricia
P
Fundación IVI-Instituto Universitario IVI, University of Valencia, and Instituto de Investigación, Sanitaria del Hospital Clinico de Valencia, Valencia, Spain.
Horcajadas
José A
JA
Martínez-Conejero
José A
JA
Esteban
Francisco J
FJ
Alamá
Pilar
P
Pellicer
Antonio
A
Simón
Carlos
C
D016000:Cluster Analysis; D004717:Endometrium / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D023281:Genomics / Q000379:methods* / Q000592:standards; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics; D008597:Menstrual Cycle / Q000502:physiology; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards; D012680:Sensitivity and Specificity; D014591:Uterine Diseases / Q000175:diagnosis* / Q000235:genetics*