title: The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration.
abstract: This study developed a bioactive knitted silk-collagen sponge scaffold by incorporation of exogenous SDF-1 alpha, to enable selective migration and homing of cells for in situ tendon regeneration. With in vitro studies, it was observed that CXCR4 gene expression and migration of bone mesenchymal stromal cells and hypo-dermal fibroblasts were more sensitive to exogenous SDF-1 alpha, while expression of tendon repair gene markers by hypo-dermal fibroblasts and Achilles tendon fibroblasts were more sensitive to exogenous SDF-1 alpha. With a rat Achilles tendon injury model, exogenous SDF-1 alpha was shown to reduce infiltration of inflammatory cells and enhance migration of fibroblast-like cells into the scaffold at 4 days and 1 week post-surgery. After 4 weeks, SDF-1 alpha treated tendon had increased expression of tendon repair gene markers and endogenous SDF-1 alpha, exhibited more physiological microstructures with larger diameter collagen fibrils, and had better biomechanical properties than the control group. Hence, our bioactive scaffold improved efficacy of tendon regeneration by increasing the recruitment of fibroblast-like cells, enhancing local endogenous SDF-1 alpha and tendon extracellular matrix production, and decreasing accumulation of inflammatory cells. Incorporation of SDF-1 alpha within a knitted silk-collagen sponge scaffold can therefore be a practical application for tendon tissue engineering.
authors.affiliation: Department of Orthopaedic Surgery, Zhejiang University, Hangzhou 310009, China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Shen
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Jialin
authors.initials: J
authors.lastname: Chen
authors.forename: Zi
authors.initials: Z
authors.lastname: Yin
authors.forename: Boon Chin
authors.initials: BC
authors.lastname: Heng
authors.forename: Weishan
authors.initials: W
authors.lastname: Chen
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D008422:Materials Testing; D051381:Rats; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D047011:Silk / Q000737:chemistry*; D013708:Tendon Injuries / Q000188:drug therapy; D013710:Tendons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D054377:Chemokine CXCL12; D047011:Silk; D003094:Collagen