title: The nitric oxide production in monocytes and macrophages of the Chlamydia trachomatis-infected patients depends on the IFN gamma and tnf alpha amount.
abstract: We studied the NO production in the peripheral blood monocytes in the Chlamydia trachomatis infected patients and in the macrophages located in the Chlamydia-caused inflammation centers. With this aim we have investigated 132 patients with Chlamydios and 85 probands for control group. The infections were identified by IFA, IFU and PCR. We have studied 20 ml venal blood and excreted monocytes by "Versan" solution. We also took the secretion from urethra and cervix areas and excreted macrophages. We distinguished monocytal and macrophage cultures and distributed them through the different schemes. The statistic elaboration of the results showed that the monocytes in the peripheral blood and the macrophages in the infected area actually secrete NO. Its inducing depends on IFN gamma and TNF alpha amounts (correlation coefficients: 0.98, p<0.05). The process increases especially under the joint influence of these cytokine (correlation coefficients: 0.91, p<0.001). Though the process seems considerably subdued in the Chlamydia-infected patients (correlation coefficients: -0.97, p<0.05). The patients seem to be affected significantly by the additional factors.
authors.affiliation: Medical Centre XXI Century, A. Tsereteli State University, Kutaisi, Georgia.
authors.forename: N
authors.initials: N
authors.lastname: Shavlakadze
authors.forename: B
authors.initials: B
authors.lastname: Gorgoshidze
mesh_terms: D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000097:blood*; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma