Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein.
HSV-1 viral capsid maturation and egress from the nucleus constitutes a self-controlled process of interactions between host cytoplasmic membrane proteins and viral capsid proteins. In this study, a member of the tetraspanin superfamily, CTMP-7, was shown to physically interact with HSV-1 protein VP26, and the VP26-CTMP-7 complex was detected both in vivo and in vitro. The interaction of VP26 with CTMP-7 plays an essential role in normal HSV-1 replication. Additionally, analysis of a recombinant virus HSV-1-UG showed that mutating VP26 resulted in a decreased viral replication rate and in aggregation of viral mutant capsids in the nucleus. Together, our data support the notion that biological events mediated by a VP26 - CTMP-7 interaction aid in viral capsid enveloping and egress from the cell during the HSV-1 infectious process.
Wang
Lei
L
Institute Of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming 650118, P. R. China.
Liu
Longding
L
Che
Yanchun
Y
Wang
Lichun
L
Jiang
Li
L
Dong
Chenghong
C
Zhang
Ying
Y
Li
Qihan
Q
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D059470:Tetraspanins; D057074:Virus Release; D014779:Virus Replication
D036022:Capsid Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C404405:TSPAN7 protein, human; D059470:Tetraspanins; C077409:capsid protein VP26, herpes simplex virus type 1