Antioxidant and free radical-scavenging activity of the extracellular death factor in Escherichia coli.
The extracellular death factor (EDF) is a newly discovered linear pentapeptide with the sequence of H-Asn-Asn-Trp-Asn-Asn-OH (NNWNN). It could act as a quorum-sensing molecule and participate in the mazEF-mediated cell death in Escherichia coli. In the present study, we firstly studied the antioxidant and free radical-scavenging activities of EDF. EDF could scavenge hydroxyl radicals in vitro and protect protein, lipid and DNA from being damaged by hydroxyl radicals. Our results indicated that this extracellular death factor might have dual effects during the programmed cell death process of Escherichia coli.
Gao
Yanfeng
Y
Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China. gaoyf@zzu.edu.cn
Chen
Kun
K
Zhang
Bin
B
Li
Xiang
X
Chen
Lu
L
Li
Yuanjun
Y
Jia
Xiangjiao
X
Lei
Yongxing
Y
Yan
Zhongyi
Z
Kong
Liangliang
L
Wang
Nan
N
Liu
Wei
W
Qi
Yuanming
Y
D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage; D004926:Escherichia coli / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D053038:Quorum Sensing; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D009842:Oligopeptides; D017392:Thiobarbituric Acid Reactive Substances; C528802:asparagyl-aspargyl-tryptophyl-asparagyl-asparagine; D013481:Superoxides; D017665:Hydroxyl Radical