title: A clinical case-control study on the association between mannose-binding lectin and susceptibility to HIV-1 infection among northern Han Chinese population.
abstract: Mannose-binding lectin (MBL) is a key molecule of the innate immune system and a competent to bind carbohydrates of a variety of microorganisms, resulting in complement activation and opsonophagocytosis against various pathogens. However, there is no systemic investigation on the MBL's role in innate immune responses against HIV-1 infection among northern Han Chinese. This study investigated the association between MBL and HIV-1 infection susceptibility among northern Han Chinese. A total of 91 HIV-1 infected patients and 91 HIV-1 seronegative healthy individuals were recruited. Six polymorphisms of MBL2 gene were genotyped by pyrosequencing. The quantitative measurement of serum MBL concentration and MBL complex activity were performed by ELISA. The CD4+ T-cell counts were determined by flow cytometry. The plasma viral loads of 91 HIV-1 infected patients were determined by bDNA method. The results show that there is an association between MBL and HIV-1 infection susceptibility among northern Han Chinese. The individuals with B variant, low serum MBL concentration and low MBL complex activity are more susceptible to HIV-1 infection.
authors.affiliation: School of Public Health and Family Medicine, Capital Medical University, Beijing, China.
authors.forename: A
authors.initials: A
authors.lastname: Sheng
authors.forename: J
authors.initials: J
authors.lastname: Lan
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Chu
authors.forename: Z
authors.initials: Z
authors.lastname: Jia
authors.forename: M
authors.initials: M
authors.lastname: Song
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Wang
mesh_terms: D000328:Adult; D044466:Asian People; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D016022:Case-Control Studies; D002681:China; D004198:Disease Susceptibility; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D015497:HIV-1; D006239:Haplotypes; D006801:Humans; D007113:Immunity, Innate; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D008875:Middle Aged; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D019562:Viral Load; D055815:Young Adult
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin