Role of hepatocyte nuclear factor-4alpha in transcriptional regulation of C1qTNF-related protein 5 in the liver.
Recently, we found that C1qTNF-related protein 5 (CTRP5) is involved in glucose and lipid metabolism. However, the transcriptional regulation of CTRP5 is largely unknown. Here we demonstrate the mechanism by which the CTRP5 gene is transcriptionally activated in the liver. CTRP5 is activated by HNF4alpha via the region -206/-167 of the human CTRP5 promoter. Deletion of this region significantly decreased HNF4alpha-induced CTRP5 promoter activity. Gel shift and chromatin immunoprecipitation analysis showed that HNF4alpha directly binds to HNF4alpha-responsive element at the region -202/-177 of the CTRP5 promoter. Taken together, these results show that hepatocyte nuclear factor-4alpha is involved in transcriptional regulation of CTRP5 in the liver.
Kim
Mi-Jin
MJ
Department of Biochemistry, School of Medicine, Dongguk University, Kyungju, Republic of Korea.
Lee
Wan
W
Park
Eun-Ju
EJ
Park
Seung-Yoon
SY
D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D005947:Glucose / Q000235:genetics / Q000378:metabolism; D051536:Hepatocyte Nuclear Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators
D051536:Hepatocyte Nuclear Factors; D011506:Proteins; D015534:Trans-Activators; C095901:transcription factor AP-5; D005947:Glucose