title: Biochemical and physiological responses in liver and muscle of rainbow trout after long-term exposure to propiconazole.
abstract: In this study, the chronic toxic effects of PCZ, a triazole fungicide commonly present in surface and ground water, on morphological indices, ROS generation and RNA/DNA ratio in liver and white muscle of rainbow trout were investigated. Fish were exposed at sublethal concentrations of PCZ (0.2, 50 and 500 microg L(-1)) for 7, 20 and 30d. Compared with the control, there were significant lower CF and HSI in fish exposed at the highest concentration of PCZ. ROS levels in both tissues increased significantly at higher PCZ concentrations (50 and 500 microg L(-1)) after 20 d and above, as well as in muscle of fish exposed at lowest PCZ concentration (0.2 microg L(-1)) after 30 d. The hepatic antioxidant enzymes (SOD and CAT) activities were induced significantly at higher concentrations (50, 500 microg L(-1)) of PCZ after 20 d and at 50 microg L(-1) after 30 d. Additional, hepatic SOD activity was significantly induced at 0.2 microg L(-1) after 30 d. Compared with the hepatic antioxidant enzymes activities in fish exposed to 50 microg L(-1) of PCZ, there was a decreasing trend in those exposed to 500 microg L(-1) after 30 d exposure. However, both the antioxidant enzymes activities were significantly inhibited in muscle of fish exposed to 500 microg L(-1) PCZ after 30 d. Moreover there was significant lower RNA/DNA ratio in both tissues after long-term exposure to higher concentration of PCZ. In short, environmental concentrations of PCZ could not induce obvious impacts on fish, but long-term exposure to higher concentrations of CBZ could affect seriously the health status of fish.
authors.affiliation: University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, Zatisi 728/II, 389 25 Vodnany, Czech Republic. zhihuali06@yahoo.com
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zlabek
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Roman
authors.initials: R
authors.lastname: Grabic
authors.forename: Josef
authors.initials: J
authors.lastname: Velisek
authors.forename: Jana
authors.initials: J
authors.lastname: Machova
authors.forename: Tomas
authors.initials: T
authors.lastname: Randak
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000378:metabolism / Q000502:physiology; D012313:RNA / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D014230:Triazoles; D014874:Water Pollutants, Chemical; C045950:propiconazole; D012313:RNA; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase