The contribution of Arabidopsis homologs of L-gulono-1,4-lactone oxidase to the biosynthesis of ascorbic acid.
To clarify the involvement of seven Arabidopsis homologs of rat L-gulono-1,4-lactone (L-GulL) oxidase, AtGulLOs, in the biosynthesis of L-ascorbic acid (AsA), transgenic tobacco cells overexpressing the various AtGulLOs were generated. Under treatment with L-GulL, the levels of total AsA in three transgenic tobacco cell lines, overexpressing AtGulLO2, 3, or 5, were significantly increased as compared with those in control cells.
Maruta
Takanori
T
Department of Advanced Bioscience, Faculty of Agriculture, Kinki University.
Ichikawa
Yaka
Y
Mieda
Takahiro
T
Takeda
Toru
T
Tamoi
Masahiro
M
Yabuta
Yukinori
Y
Ishikawa
Takahiro
T
Shigeoka
Shigeru
S
D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism*; D001205:Ascorbic Acid / Q000096:biosynthesis*; D002460:Cell Line; D050544:L-Gulonolactone Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D051381:Rats; D017385:Sequence Homology; D014026:Nicotiana / Q000166:cytology / Q000235:genetics
D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid