title: Two dogs with juvenile-onset skin diseases with involvement of extremities.
abstract: Two dogs of juvenile-onset skin diseases with involvement of extremities were examined by histopathological, immunohistochemical and ultrastructural analyses. Clinically, both cases showed alopecia and crusts on the face and extremities. Case 1 showed histopathology of dermo-epidermal separation. Ultrastructural analysis revealed that the clefts were recognized between hemidesmosomes and lamina densa. In case 2, histopathology showed follicular atrophy, vacuolar degeneration at lower epidermis and masseter muscle degeneration, without remarkable ultrastructural abnormalities in basement membrane zone of the skin. Thus, the findings in case 1 were compatible to those in junctional epidermolysis bullosa, while those in case 2 were compatible to dermatomyositis-like disease. Combination of histopathological and ultrastructural analyses was useful to distinguish the diseases in two dogs.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Yoon
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Minami
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Takizawa
authors.forename: Maiko
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yabuzoe
authors.forename: Kaori
authors.initials: K
authors.lastname: Ide
authors.forename: Koji
authors.initials: K
authors.lastname: Nishifuji
authors.forename: Toshiroh
authors.initials: T
authors.lastname: Iwasaki
mesh_terms: D017668:Age of Onset; D000505:Alopecia / Q000473:pathology / Q000662:veterinary; D000818:Animals; D017931:DNA Primers; D020405:Dermis / Q000473:pathology / Q000648:ultrastructure; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D004817:Epidermis / Q000473:pathology / Q000648:ultrastructure; D016109:Epidermolysis Bullosa, Junctional / Q000473:pathology / Q000662:veterinary; D007797:Laminin / Q000235:genetics; D008406:Masseter Muscle / Q000473:pathology / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D012871:Skin Diseases / Q000473:pathology / Q000662:veterinary*
chemical_list: D017931:DNA Primers; D007797:Laminin