Whole human exome capture for high-throughput sequencing.
We captured the whole human exome by hybridization using synthesized oligonucleotides, based on a high-density microarray design, and we sequenced those captured human exons using high-throughput sequencing on a Genome Sequencer FLX instrument. Of the uniquely mapped reads, 71% fell within target regions, and these corresponded to coverage of 94% of human genes, 87% of exons, and 70% of the total base-pair length of the CCDS set. Our study provides strong evidence for the practical usefulness of this method on a genome-wide scale, showing the resequenced whole human exome database with 501 microRNAs and 307 novel SNPs.
Kim
Dae-Won
DW
Division of Malaria and Parasitic Diseases, National Institute of Health, Seoul, Korea.
Nam
Seong-Hyeuk
SH
Kim
Ryong Nam
RN
Choi
Sang-Haeng
SH
Park
Hong-Seog
HS
D002874:Chromosome Mapping; D005091:Exons / Q000235:genetics*; D015894:Genome, Human; D023281:Genomics; D057166:High-Throughput Screening Assays; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA
D035683:MicroRNAs