title: Processing of the dynamin Msp1p in S. pombe reveals an evolutionary switch between its orthologs Mgm1p in S. cerevisiae and OPA1 in mammals.
abstract: Mitochondrial fusion depends on the evolutionary conserved dynamin, OPA1/Mgm1p/Msp1p, whose activity is controlled by proteolytic processing. Since processing diverges between Mgm1p (Saccharomyces cerevisiae) and OPA1 (mammals), we explored this process in another model, Msp1p in Schizosaccharomyces pombe. Generation of the short isoform of Msp1p neither results from the maturation of the long isoform nor correlates with mitochondrial ATP levels. Msp1p is processed by rhomboid and a protease of the matrix ATPase associated with various cellular activities (m-AAA) family. The former is involved in the generation of short Msp1p and the latter in the stability of long Msp1p. These results reveal that Msp1p processing may represent an evolutionary switch between Mgm1p and OPA1.
authors.affiliation: Université de Toulouse, UPS CNRS UMR5241, Laboratoire Métabolisme Plasticité Mitochondries, Toulouse, France.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Leroy
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Khosrobakhsh
authors.forename: Alan
authors.initials: A
authors.lastname: Diot
authors.forename: Marlène
authors.initials: M
authors.lastname: Daloyau
authors.forename: Laetitia
authors.initials: L
authors.lastname: Arnauné-Pelloquin
authors.forename: Cindy
authors.initials: C
authors.lastname: Cavelier
authors.forename: Laurent J
authors.initials: LJ
authors.lastname: Emorine
authors.forename: Pascale
authors.initials: P
authors.lastname: Belenguer
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005075:Biological Evolution; D034281:Dynamins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D008561:Membrane Fusion / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D029241:Optic Atrophy, Autosomal Dominant / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D000251:Adenosine Triphosphatases; D034281:Dynamins