Modules for C-terminal epitope tagging of Tetrahymena genes.
Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.
Kataoka
Kensuke
K
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohr-Gasse 3, A-1030 Vienna, Austria.
Schoeberl
Ursula E
UE
Mochizuki
Kazufumi
K
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D013768:Tetrahymena / Q000737:chemistry / Q000235:genetics*
D015800:Protozoan Proteins