Oxidative stress induced by 2,4-phenoxyacetic acid in liver of female rats and their progeny: biochemical and histopathological studies.
The 2,4-Dichlorophenoxyacetic acid (2,4-D) was used in agriculture as an herbicide in many countries including Tunisia. The aim of this study was to evaluate the effects of 2,4-D on liver function of adult rats and their progeny. Female Wistar rats were divided into two groups: the controls and the treated rats which received 600 ppm of 2,4-D in their drinking water from the 14th day of pregnancy until day 14 after delivery. In 2,4-D group, a significant decrease in body weight of pups was noted, when compared to controls. Liver antioxidant enzyme activities, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) decreased, while malondialdehyde (MDA) levels increased in dams and pups. Moreover, plasma aminotransferases (ALT, AST), gamma glutamil transpeptidase (GGT), lactate dehydrogenase (LDH), bilirubin and albumin levels were increased significantly. The biochemical modifications were correlated with histopathological studies. We concluded that 2,4-D induced hepatotoxicity in adult and suckling rats.
Troudi
Afef
A
Animal Physiology Laboratory, UR 08-73 Sfax Faculty of Sciences, BP 1171, Sfax 3000, Tunisia.
Ben Amara
Ibtissem
I
Samet
Amira Mahjoubi
AM
Zeghal
Najiba
N
D015084:2,4-Dichlorophenoxyacetic Acid / Q000008:administration & dosage / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002374:Catalase / Q000378:metabolism; D060766:Drinking Water / Q000737:chemistry; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014416:Tunisia
D060766:Drinking Water; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000410:Alanine Transaminase