Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8.
Aquaporin (AQP) family plays a fundamental role in transmembrane water and small solutes movement. Within this family, aquaporin 8 (AQP8), showed to be widely distributed in the digestive system especially colon. To investigate the possible protein alterations involved in AQP8 regulation and trafficking, we extensively compared between wild type and AQP8 knockout mouse colon using semi-quantitative fluorescence- stained two dimensional gel electrophoresis (2-DE) coupled with nano LC-Ms/Ms. Our analysis revealed identification and regulation of 21 proteins, most notably, actin-related family which suggests its possible involvement in regulating AQP8 secretory vesicles migration to be integrated as a cell membrane protein.
Magdeldin
Sameh
S
Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-dori, Japan. samehmagd@med.niigata-u.ac.jp
Li
Huiping
H
Yoshida
Yutaka
Y
Enany
Shymaa
S
Zhang
Ying
Y
Xu
Bo
B
Fujinaka
Hidehiko
H
Yaoita
Eishin
E
Yamamoto
Tadashi
T
D000818:Animals; D020346:Aquaporins / Q000235:genetics*; D003106:Colon / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D007525:Isoelectric Focusing; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020543:Proteome / Q000378:metabolism
D020346:Aquaporins; D020543:Proteome; C108330:aquaporin 8