title: Effect of bioaggregate on mineral-associated gene expression in osteoblast cells.
abstract: BACKGROUND
  This study investigated the cytotoxicity of bioaggregate (BA) and the effect of BA on mineral associated gene expression in osteoblast cells.
  
  
  METHODS
  The cytotoxicity of BA to mouse MC3T3-E1 osteoblast cells was evaluated via the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 1, 2, and 3 days of culture. The expression of mineral associated genes (collagen type 1, osteocalcin, and osteopontin) was assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and compared expression after exposure to BA or mineral trioxide aggregate (MTA). The data were analyzed by one-way analysis of variance and the Tukey test.
  
  
  RESULTS
  BA was essentially nontoxic to osteoblast cells. The expression of collagen type 1, osteocalcin, and osteopontin genes significantly increased in the BA group compared with that in the MTA group on the second or third day of culture.
  
  
  CONCLUSIONS
  BA appears to be a novel nontoxic root-end filling biomaterial and be able to induce mineralization-associated gene expression in osteoblast cells.
authors.affiliation: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Zhenglin
authors.initials: Z
authors.lastname: Yuan
authors.forename: Bin
authors.initials: B
authors.lastname: Peng
authors.forename: Han
authors.initials: H
authors.lastname: Jiang
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Bian
authors.forename: Ping
authors.initials: P
authors.lastname: Yan
mesh_terms: D016475:3T3 Cells; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D004396:Coloring Agents; D004338:Drug Combinations; D017886:Durapatite / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008903:Minerals / Q000032:analysis; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D010087:Oxides / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D024042:Collagen Type I; D004396:Coloring Agents; D004338:Drug Combinations; D008903:Minerals; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C506896:Spp1 protein, mouse; D013778:Tetrazolium Salts; D013844:Thiazoles; C086631:mineral trioxide aggregate; D015675:Osteocalcin; D053495:Osteopontin; D017886:Durapatite; C022616:thiazolyl blue; D002126:Calcium Hydroxide; C031293:calcium silicate