title: Frequency and phenotype of T helper 17 cells in peripheral blood and synovial fluid of patients with reactive arthritis.
abstract: OBJECTIVE
  To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA).
  
  
  METHODS
  T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry.
  
  
  RESULTS
  The percentages of interleukin 17 (IL-17)-positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10.
  
  
  CONCLUSIONS
  These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.
authors.affiliation: First Affiliated Hospital of China Medical University - Rheumatology, Shen Yang, China. shenhuicam@sohu.com
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.forename: Jane C
authors.initials: JC
authors.lastname: Goodall
authors.forename: J S Hill
authors.initials: JS
authors.lastname: Gaston
mesh_terms: D016918:Arthritis, Reactive / Q000097:blood / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology*; D010641:Phenotype; D000091402:Prohibitins; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D020381:Interleukin-17; C000717331:PHB2 protein, human; D000091402:Prohibitins