Rapid detection of BF haplotypes by a semi-nested polymerase chain reaction, which causes resistance/susceptibility to Marek's disease in chicken.
A semi-nested polymerase chain reaction (snPCR) assay was developed for the rapid detection of resistant/susceptible BF haplotypes to Marek's disease (MD) using the cDNA samples from peripheral blood leucocytes, liver, spleen and heart from Xiayan homozygous chickens: A(11), C(23), D(8) and D(12) (resistant to MD), A(5) and B(21) (susceptible to MD). The snPCR was utilized to span alternative splicing-out of the sequence encoding the second segment of the cytoplasmic part of the mature BF molecules (exon 7). This alternative exon 7 splice variant was detected in BF*A(5) and BF*B(21) (susceptible to MD), but not in the MD-resistant BF*A(11), BF*C(23), BF*D(8) and BF*D(12) haplotypes, suggesting a potential role of exon 7 for the detection of resistant/susceptible BF haplotypes to MD.
Jin
Y-C
YC
Poultry Science and Health, Guangxi University, Nanning, China.
Wei
P
P
Wei
X-X
XX
Zhao
Z-Y
ZY
Li
Y
Y
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D008380:Marek Disease / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D000949:Histocompatibility Antigens Class II; D012313:RNA