title: Repeated fed-batch lactic acid production in a packed bed-stirred fermentor system using a pH feedback feeding method.
abstract: Lactic acid production by repeated fed-batch fermentation using free and immobilized cells of Lactobacillus lactis-11 in a packed bed-stirred fermentor (PBSF) system filled with different support materials including ceramic beads, macro-activated carbon cylinders and glass fiber balls was investigated. The results showed that the optimal support materials were the ceramic beads with diameters of 1-2 mm. Compared with the free cell fermentation system, lactic acid production and volumetric productivity in the PBSF system increased by 16.6 and 12.5%, respectively. Though the concentration of free cells decreased sharply, lactic acid production remained stable in five consecutive fed-batch runs using the PBSF system. pH gradients, immobilized cell concentration and mass diffusion in the packed bed were all affected by the recirculation rate of the culture broth. Maximum lactic acid production, productivity and yield occurred at a recirculation rate of 50 mL min(-1).
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, PO Box 353, Beijing 100190, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Cong
authors.forename: Shao Yuan
authors.initials: SY
authors.lastname: Shi
mesh_terms: D019149:Bioreactors; D018914:Cells, Immobilized / Q000378:metabolism*; D002516:Ceramics; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000096:biosynthesis*; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*
chemical_list: D019344:Lactic Acid