title: Mapping of quantitative trait loci for high level of self-incompatibility in Brassica rapa L.
abstract: The level of self-incompatibility (SI) is important to the purity of F1 seeds produced using the SI system of Brassica vegetables. To analyze the genetic basis of the level of SI, we generated an F2 population derived from a cross between a turnip inbred line showing a high level of SI and a Chinese cabbage inbred line showing a low level, and evaluated the level of SI under insect pollination in two years. We constructed a detailed linkage map of Brassica rapa from the F2 progeny, consisting of SSR, SNP, indel, and CAPS loci segregating into 10 linkage groups covering approximately 700 cM. Five quantitative trait loci (QTL) for high-level SI were identified. The phenotypic variation explained by the QTL ranged between 7.2% and 23.8%. Two QTL were detected in both years. Mapping of SI-related genes revealed that these QTL were co-localized with SLG on R07 and MLPK on R03. This is the first report of QTL for high-level SI evaluated under insect pollination in a Brassica vegetable. Our results could be useful for the marker-assisted selection of parental lines with a stable SI.
authors.affiliation: National Institute of Vegetable and Tea Science (NIVTS), 360, Kusawa, Ano, Tsu, Mie 514-2392, Japan. khatake@affrc.go.jp
authors.forename: Katsunori
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Horisaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Niikura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Narusaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yoshiaki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ishida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukuoka
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D029741:Brassica rapa / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D007178:Inbreeding; D007313:Insecta / Q000502:physiology; D054817:Pollination / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers