Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice.
It has been speculated that the mandibular condyle develops via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes; however, the developmental mechanisms behind this process have not been revealed. We used laser-capture microdissection and cDNA microarray analysis to elucidate the genes that are highly expressed in these fibroblast-like cells. Among these genes, the transcription of Ten-m/Odz3 was significantly increased in the fibroblast-like cells compared with other cartilage tissues. For the first time, we describe the temporal and spatial expression of Ten-m/Odz3 mRNA in relation to the expression of type I, II, and X collagen mRNA, as determined by in-situ hybridization in mouse mandibular condylar cartilage and mouse femoral cartilage during the early stages of development. Ten-m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, which expressed type I and II collagen, respectively, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in-vitro expression of Ten-m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.
Murakami
Takashi
T
Department of Orthodontics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Fukunaga
Tomohiro
T
Takeshita
Nobuo
N
Hiratsuka
Koichi
K
Abiko
Yoshimitsu
Y
Yamashiro
Takashi
T
Takano-Yamamoto
Teruko
T
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008335:Mandibular Condyle / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C525233:Tenm3 protein, mouse; D003094:Collagen