title: Study of effect of molecular mobility in chromatophore membranes of the bacterium E. shaposhnikovii on processes of photoinduced electron transport using the NMR-spin-echo method with isotope substitution and dehydration.
abstract: The effect of dehydration and (2)H2O/H2O isotope substitution on electron transport reactions and relaxation of proton-containing groups was studied in chromatophore membranes of Ectothiorhodospira shaposhnikovii. During dehydration (including isotope substitution of hydrate water) of preliminarily dehydrated isolated photosynthetic membranes there was a partial correlation between hydration intervals within which activation of electron transport from high-potential cytochrome c to photoactive bacteriochlorophyll dimer P890 of photosynthetic reaction center and variation of spin-lattice and spin-spin proton relaxation time was observed. Partial correlation between hydration intervals can be considered as evidence of correlation between mobility of non-water proton-containing groups with proton relaxation frequency approximately 10(8) sec(-1) with efficiency of electron transfer at the donor side of the chain.
authors.affiliation: Department of Biophysics, Biology Faculty, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: C S
authors.initials: CS
authors.lastname: Chamorovsky
authors.forename: S K
authors.initials: SK
authors.lastname: Chamorovsky
authors.forename: P P
authors.initials: PP
authors.lastname: Knox
mesh_terms: D020130:Bacterial Chromatophores / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D003903:Deuterium / Q000737:chemistry; D020608:Ectothiorhodospira shaposhnikovii / Q000378:metabolism*; D004579:Electron Transport; D024202:Electrophoretic Mobility Shift Assay; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010084:Oxidation-Reduction; D011789:Quantum Theory
chemical_list: D045304:Cytochromes c; D003903:Deuterium