Fe(3)O(4) nanoparticles-loaded PEG-PLA polymeric vesicles as labels for ultrasensitive immunosensors.
A class of immuno-labels based on poly(ethylene glycol)-poly(lactic acid) (PEG-PLA) polymeric vesicles was developed in this study. To fabricate these immune-labels, the uniform Fe(3)O(4) nanoparticles (NPs) were loaded into the vesicles followed by conjugating secondary antibody (Ab(2)) onto the vesicles surface, named Ab(2)-PEG-PLA-Fe(3)O(4). The resulting Ab(2)-PEG-PLA-Fe(3)O(4) demonstrated high catalytic activity towards H(2)O(2), and the sensitivity of the sandwich-type immunosensor using this label for prostate specific antigen (PSA) detection increased greatly. The immunosensor based on this label exhibited high sensitivity, wide linear range (0.005-10 ng/mL), low detection limit (2 pg/mL), good reproducibility, selectivity and stability. These labels for immunosensors may provide many potential applications for the ultrasensitive detection of different cancer biomarkers. In addition, this technique also has the potential to be extended to the loading of other interesting material for preparing various kinds of labels to meet the different requirements in immunoassays.
Wei
Qin
Q
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, People's Republic of China.
Li
Ting
T
Wang
Gaolei
G
Li
He
H
Qian
Zhiyong
Z
Yang
Minghui
M
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D004337:Drug Carriers / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D000906:Antibodies; D055910:Antibodies, Immobilized; D001672:Biocompatible Materials; D004337:Drug Carriers; D005290:Ferric Compounds; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide)