Characterization of hematopoietic potential of mesenchymal stem cells.
Mesenchymal and hematopoietic tissues are important reservoirs of adult stem cells. The potential of tissue resident mesenchymal stem cells (MSCs) to differentiate into cells of mesodermal and ectodermal lineages has been reported previously. We examined the hypothesis that adherent adipose tissue resident mesenchymal stem cells (ASCs) are capable of generating cells with hematopoietic characteristics. When cultured in differentiation media, clonally isolated ASCs develop into cells with hematopoietic attributes. The hematopoietic differentiated cells (HD) express early hematopoietic (c-kit, PROM1, CD4) as well as monocyte/macrophage markers (CCR5, CD68, MRC1, CD11b, CSF1R). Additionally, HD cells display functional characteristics of monocyte/macrophages such as phagocytosis and enzymatic activity of α-Naphthyl Acetate Esterase. HD cells are also responsive to stimulation by IL-4 and LPS as shown by increased CD14 and HLA-DRB1 expressions and release of IL-2, IL10, and TNF. Taken together, this study characterizes the potential of ASCs to generate functional macrophages in vitro, and therefore paves way for their possible use in cell therapy applications.
Freisinger
Eva
E
Department of Medicine, Applied Stem Cell Laboratory, Heart and Vascular Institute, Tulane University Health Science Center, New Orleans, Louisiana 70112, USA.
Cramer
Christopher
C
Xia
Xiujin
X
Murthy
Subramanyam N
SN
Slakey
Douglas P
DP
Chiu
Ernest
E
Newsome
Edward R
ER
Alt
Eckhard U
EU
Izadpanah
Reza
R
D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D002999:Clone Cells; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D009283:Naphthol AS D Esterase / Q000378:metabolism; D010587:Phagocytosis; D013997:Time Factors
D015415:Biomarkers; D009283:Naphthol AS D Esterase