[The expression and role of Cathepsin B in intracranial aneurysm wall].
OBJECTIVE
To detect the expression of Cathepsin B (CatB) in the intracranial aneurysm wall and its effect to the apoptosis of vascular smooth muscle cells, aimed at clarifying the pathological formation mechanism of intracranial aneurysm.


METHODS
From November 2006 to February 2009, 20 intracranial aneurysm samples were collected as the experimental group, and 6 cases of normal pallium artery samples were collected as the control group. Immunohistochemical technique was used to evaluate the expressions of CatB, alpha-smooth muscle actin (alpha-SMA) and Caspase-3. The expression of CatB mRNA was evaluated by real-time PCR. The ultrastructure of intracranial aneurysms were observed by using the transmission electronic microscope.


RESULTS
Compared with the normal pallium artery specimens, the expression of CatB and Caspase-3 both significantly increased in the intracranial aneurysm walls where alpha-SMA decreased (P < 0.05). The mean expression of CatB mRNA in intracranial aneurysm samples was about 3.8-folds than that in control group (P < 0.01). There were excessive apoptotic vascular smooth muscle cells (VSMCs) in the tunica median, and typical apoptotic body were observed in some aneurysm walls.


CONCLUSIONS
Cathepsin B may be involved in the formation and the progression of intracranial aneurysm.
Yu
Nian-zu
NZ
Department of Surgery, the Childzen's Hospital of Jiangxi Province, Nanchang 330006, China.
Wang
Zhong
Z
Zhao
Hong-xiang
HX
Wu
Yu
Y
Zhou
Peng
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D016022:Case-Control Studies; D002401:Cathepsin B / Q000378:metabolism*; D005260:Female; D006801:Humans; D002532:Intracranial Aneurysm / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D055815:Young Adult
D002401:Cathepsin B