title: Tegumental surface changes in adult Fasciola hepatica in response to treatment in vivo with triclabendazole in the sheep host.
abstract: Eight indoor-reared crossbred sheep with no pre-exposure to Fasciola hepatica were infected by oral gavage with 200 metacercarial cysts of the triclabendazole (TCBZ)-susceptible Cullompton isolate of F. hepatica. At 12 weeks post-infection, sheep were dosed with 10mg/kg triclabendazole. Two sheep per time period were euthanized at 48h, 72h and 96h post-treatment. Two control sheep were euthanized alongside the 96h triclabendazole-treated sheep. Flukes were recovered from each of the sheeps' liver and, if present, from the gall bladder, and they were processed for scanning electron microscopy (SEM). Flukes recovered 48h post-treatment were active. Disruption to the tegument took the form of swelling, widespread blebbing and some loss of the tegument covering the spines. By 72h post-treatment, all flukes recovered were dead and a number were recovered from the gall bladder. Typically, the posterior end of the flukes was elongated and in this region the tegumental syncytium had sloughed away. Tegumental loss was more widespread on flukes recovered from the gall bladder. At 96h post-treatment, only one fluke was recovered from the liver and three from the gall bladder. All the flukes were dead and they were totally devoid of tegumental syncytium.
authors.affiliation: Parasite Therapeutics Research Group, Medical Biology Centre, The Queen's University of Belfast, Belfast, BT9 7BL, UK.
authors.forename: E
authors.initials: E
authors.lastname: Toner
authors.forename: G P
authors.initials: GP
authors.lastname: Brennan
authors.forename: R E B
authors.initials: RE
authors.lastname: Hanna
authors.forename: H W
authors.initials: HW
authors.lastname: Edgar
authors.forename: I
authors.initials: I
authors.lastname: Fairweather
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology* / Q000627:therapeutic use*; D005210:Fasciola hepatica / Q000187:drug effects* / Q000648:ultrastructure; D005211:Fascioliasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D005704:Gallbladder / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008855:Microscopy, Electron, Scanning; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology*; D000077682:Triclabendazole
chemical_list: D001562:Benzimidazoles; D000077682:Triclabendazole