title: Laboratory surveillance for prospective plasmid-mediated AmpC beta-lactamases in the Kinki region of Japan.
abstract: Extended-spectrum beta-lactamases, plasmid-mediated AmpC beta-lactamases (PABLs), and plasmid-mediated metallo-beta-lactamases confer resistance to many beta-lactams. In Japan, although several reports exist on the prevalence of extended-spectrum beta-lactamases and metallo-beta-lactamases, the prevalence and characteristics of PABLs remain unknown. To investigate the production of PABLs, a total of 22,869 strains of 4 enterobacterial species, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis, were collected during six 6-month periods from 17 clinical laboratories in the Kinki region of Japan. PABLs were detected in 29 (0.13%) of 22,869 isolates by the 3-dimensional test, PCR analysis, and DNA sequencing analysis. PABL-positive isolates were detected among isolates from 13 laboratories. Seventeen of 13,995 (0.12%) E. coli isolates, 8 of 5,970 (0.13%) K. pneumoniae isolates, 3 of 1,722 (0.17%) K. oxytoca isolates, and 1 of 1,182 (0.08%) P. mirabilis isolates were positive for PABLs. Of these 29 PABL-positive strains, 20 (69.0%), 6 (20.7%), 2 (6.9%), and 1 (3.4%) carried the genes for CMY-2, DHA-1, CMY-8, and MOX-1 PABLs, respectively. Pattern analysis of randomly amplified polymorphic DNA and pulsed-field gel electrophoretic analysis revealed that the prevalence of CMY-2-producing E. coli strains was not due to epidemic strains and that 3 DHA-1-producing K. pneumoniae strains were identical, suggesting their clonal relatedness. In conclusion, the DHA-1 PABLs were predominantly present in K. pneumoniae strains, but CMY-2 PABLs were predominantly present in E. coli strains. The present findings will provide significant information to assist in preventing the emergence and further spread of PABL-producing bacteria.
authors.affiliation: Department of Clinical Laboratory, Wakayama Rosai Hospital, 93-1 Kinomoto, Wakayama City, Wakayama 640-8505, Japan. ktt-yamazaki@wakayamah.rofuku.go.jp
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Masaru
authors.initials: M
authors.lastname: Komatsu
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Abe
authors.forename: Saori
authors.initials: S
authors.lastname: Fukuda
authors.forename: Yugo
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Higuchi
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Ono
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Nishio
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sueyoshi
authors.forename: Kaneyuki
authors.initials: K
authors.lastname: Kida
authors.forename: Kaori
authors.initials: K
authors.lastname: Satoh
authors.forename: Masahiro
authors.initials: M
authors.lastname: Toyokawa
authors.forename: Isao
authors.initials: I
authors.lastname: Nishi
authors.forename: Masako
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Akagi
authors.forename: Isako
authors.initials: I
authors.lastname: Nakai
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kofuku
authors.forename: Tamaki
authors.initials: T
authors.lastname: Orita
authors.forename: Yasunao
authors.initials: Y
authors.lastname: Wada
authors.forename: Takumi
authors.initials: T
authors.lastname: Jikimoto
authors.forename: Shohiro
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Itaru
authors.initials: I
authors.lastname: Hirai
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D010957:Plasmids / Q000032:analysis*; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D047090:beta-Lactams; C048795:AmpC beta-lactamases; D001618:beta-Lactamases