title: Adipocyte-derived microvesicles contain RNA that is transported into macrophages and might be secreted into blood circulation.
abstract: We have recently found that 3T3-L1 adipocytes secrete microvesicles, known as adipocyte-derived microvesicles (ADMs), with angiogenic activity. In this study, we found that ADMs contain RNA without typical 28S and 18S ribosomal RNA inside the vesicles. Microarray analysis revealed that ADMs contain approximately 7000 mRNAs and 140 microRNAs. Most of transcripts for adipocyte-specific and dominant genes were contained in the ADMs, and their abundance was mostly correlated with that in the donor cells. Abundance of adipocyte-related microRNAs was also mostly correlated with that in the donor cells. ADMs mediated transport of adiponectin and resistin gene transcripts into RAW264.7 macrophages. Moreover, adipocyte-specific gene transcripts such as adiponectin, resistin, and PPARgamma2 were found in microvesicles isolated from rat serum. Thus, ADM might play a role as a novel intercellular communication tool by transporting RNA in paracrine and possibly endocrine manners.
authors.affiliation: Department of Life Science, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu 514-8507, Japan.
authors.forename: Rumiko
authors.initials: R
authors.lastname: Ogawa
authors.forename: Chie
authors.initials: C
authors.lastname: Tanaka
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sato
authors.forename: Haruka
authors.initials: H
authors.lastname: Nagasaki
authors.forename: Kazuto
authors.initials: K
authors.lastname: Sugimura
authors.forename: Katsuzumi
authors.initials: K
authors.lastname: Okumura
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000097:blood / Q000235:genetics; D000818:Animals; D001692:Biological Transport; D001775:Blood Circulation; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D047495:PPAR gamma / Q000097:blood / Q000235:genetics; D019899:Paracrine Communication; D012333:RNA, Messenger / Q000097:blood* / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB; D052243:Resistin / Q000097:blood / Q000235:genetics; D022142:Secretory Vesicles / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D052242:Adiponectin; D047495:PPAR gamma; D012333:RNA, Messenger; D052243:Resistin