Synthesis and properties of fluorescence probe for detection of peroxides in mitochondria.
In this study, a new type of fluorescence probe, diphenylpyrenylphosphine-conjugated alkyltriphenylphosphonium iodide which was accumulated in mitochondria, has been synthesized. This probe was detected peroxide in living cell. Comparison of the reactivity toward several peroxide indicated that the probe was existed in mitochondrial membrane. Using this probe, generation of peroxide in mitochondria of living cell was successfully visualized.
Shioji
Kosei
K
Department of Chemistry, Fukuoka University, Jonan-Ku, Japan. shioji@fukuoka-u.ac.jp
Oyama
Yu
Y
Okuma
Kentaro
K
Nakagawa
Hiroyuki
H
D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D010545:Peroxides / Q000032:analysis*; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D010545:Peroxides