The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1.
The positive-stranded RNA genome of classical swine fever virus (CSFV) encodes 12 known proteins. The first protein to be translated is the N-terminal protease (N(pro)). N(pro) helps evade the innate interferon response by targeting interferon regulatory factor-3 for proteasomal degradation and also participates in the evasion of dsRNA-induced apoptosis. To elucidate the mechanisms by which N(pro) functions, we performed a yeast two-hybrid screen in which the anti-apoptotic protein HAX-1 was identified. The N(pro)-HAX-1 interaction was confirmed using co-precipitation assays. A dramatic redistribution of both N(pro) and HAX-1 was observed in co-transfected cells, as well as in transfected cells infected with wild-type CSFV, but not in cells infected with an N(pro)-deleted CSFV strain.
Johns
Helen L
HL
Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, UK.
Doceul
Virginie
V
Everett
Helen
H
Crooke
Helen
H
Charleston
Bryan
B
Seago
Julian
J
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000472:pathogenicity*; D010450:Endopeptidases / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D013552:Swine; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C104759:HAX1 protein, human; D014764:Viral Proteins; D010450:Endopeptidases; C505229:N(pro) protein, swine fever virus