Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe.
We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.
Liu
Gangjun
G
Beckman Laser Institute, University of California, Irvine, Irvine, California 92612, USA. gangjun@gmail.com
Kieu
Khanh
K
Wise
Frank W
FW
Chen
Zhongping
Z
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D057054:Molecular Imaging; D017785:Photons; D051381:Rats; D013710:Tendons / Q000166:cytology; D013997:Time Factors
D005456:Fluorescent Dyes