title: Analysis of genes, transcripts, and proteins via DNA ligation.
abstract: Analytical reactions in which short DNA strands are used in combination with DNA ligases have proven useful for measuring, decoding, and locating most classes of macromolecules. Given the need to accumulate large amounts of precise molecular information from biological systems in research and in diagnostics, ligation reactions will continue to offer valuable strategies for advanced analytical reactions. Here, we provide a basis for further development of methods by reviewing the history of analytical ligation reactions, discussing the properties of ligation reactions that render them suitable for engineering novel assays, describing a wide range of successful ligase-based assays, and briefly considering future directions.
authors.affiliation: Department of Genetics and Pathology, The Rudbeck Lab, Uppsala University, Uppsala, Sweden.
authors.forename: Tim
authors.initials: T
authors.lastname: Conze
authors.forename: Alysha
authors.initials: A
authors.lastname: Shetye
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Jijuan
authors.initials: J
authors.lastname: Gu
authors.forename: Chatarina
authors.initials: C
authors.lastname: Larsson
authors.forename: Jenny
authors.initials: J
authors.lastname: Göransson
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Tavoosidana
authors.forename: Ola
authors.initials: O
authors.lastname: Söderberg
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D011088:DNA Ligases / Q000737:chemistry / Q000378:metabolism*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D008967:Molecular Biology / Q000266:history / Q000379:methods*
chemical_list: D004247:DNA; D011088:DNA Ligases