Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum).
Metallothionein type 3 (MT3) expression has previously been detected in leaves, fruits, and developing somatic embryos in different plant species. However, specific tissular and cellular localization of MT3 transcripts have remained unidentified. In this study, in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements, mesophyll and guard cells of leaves, vascular tissue of roots and throughout the whole embryo. Changes in FeMT3 mRNA levels in response to drought and oxidative stress, as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected, indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes.
Samardzić
Jelena T
JT
Department of Plant Molecular Biology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia. jelena.samardzic@imgge.bg.ac.rs
Nikolić
Dragana B
DB
Timotijević
Gordana S
GS
Jovanović
Zivko S
ZS
Milisavljević
Mira Đ
MĐ
Maksimović
Vesna R
VR
D055864:Droughts; D019612:Fagopyrum / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D010940:Plant Proteins; D017382:Reactive Oxygen Species; D008668:Metallothionein