title: Recombinant peroxidase production in species of lepidoptera frequently found in Argentina.
abstract: Horseradish peroxidase isozyme C (HRPC) is an important commercial biocatalyst. In this study, a screening of different lepidopteran species frequently found in Argentina to produce this protein was carried out. Two recombinant viruses were constructed: AcMNPV HRPC polyhedrin-minus (occ-), an intrahemocelical infective virus; and AcMNPV HRPC polyhedrin-plus (occ+), to achieve an oral infective baculovirus. Each lepidopteran species was infected either with AcMNPV HRPC occ- or AcMNPV HRPC occ+ and the harvesting days post-infection (dpi) were optimized. All species were susceptible to AcMNPV HRPC occ- infection, giving Spodoptera frugiperda the best yield: 41 μg per larva. Rachiplusia nu was highly susceptible to oral infection, reaching 22 μg per larva at 4 dpi. HRPC was purified by IMAC from S. frugiperda extracts with a yield of 86% and a purification factor of 29.
authors.affiliation: Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica (UBA), Junín 956, 1113, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Romero
authors.forename: A
authors.initials: A
authors.lastname: Targovnik
authors.forename: F
authors.initials: F
authors.lastname: Wolman
authors.forename: M
authors.initials: M
authors.lastname: Fogar
authors.forename: M
authors.initials: M
authors.lastname: Simonella
authors.forename: O
authors.initials: O
authors.lastname: Cascone
authors.forename: M
authors.initials: M
authors.lastname: Miranda
mesh_terms: D000818:Animals; D001118:Argentina; D006735:Horseradish Peroxidase / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000201:enzymology / Q000821:virology; D007915:Lepidoptera / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics / Q000821:virology*
chemical_list: D019476:Insect Proteins; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase