title: Crystallization and preliminary X-ray crystallographic analysis of BxlA, an intracellular beta-D-xylosidase from Streptomyces thermoviolaceus OPC-520.
abstract: BxlA from Streptomyces thermoviolaceus OPC-520, together with the extracellular BxlE and the integral membrane proteins BxlF and BxlG, constitutes a xylanolytic system that participates in the intracellular transport of xylan-degradation products and the production of xylose. To elucidate the mechanism of the hydrolytic degradation of xylooligosaccharides to xylose at the atomic level, X-ray structural analysis of BxlA was attempted. The recombinant BxlA protein (molecular weight 82 kDa) was crystallized by the hanging-drop vapour-diffusion method at 289 K. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 142.2, b = 129.5, c = 101.4 A, beta = 119.8 degrees , and contained two molecules per asymmetric unit (V(M) = 2.47 A(3) Da(-1)). Diffraction data were collected to a resolution to 2.50 A and provided a data set with an overall R(merge) of 8.3%.
authors.affiliation: Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Morioka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miki
authors.forename: Kei
authors.initials: K
authors.lastname: Saito
authors.forename: Koji
authors.initials: K
authors.lastname: Tomoo
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Ishida
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Akihito
authors.initials: A
authors.lastname: Yamano
authors.forename: Chiaki
authors.initials: C
authors.lastname: Takada
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D013302:Streptomyces / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry*
chemical_list: D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase