title: Role of hepatocyte nuclear factor-4alpha in transcriptional regulation of C1qTNF-related protein 5 in the liver.
abstract: Recently, we found that C1qTNF-related protein 5 (CTRP5) is involved in glucose and lipid metabolism. However, the transcriptional regulation of CTRP5 is largely unknown. Here we demonstrate the mechanism by which the CTRP5 gene is transcriptionally activated in the liver. CTRP5 is activated by HNF4alpha via the region -206/-167 of the human CTRP5 promoter. Deletion of this region significantly decreased HNF4alpha-induced CTRP5 promoter activity. Gel shift and chromatin immunoprecipitation analysis showed that HNF4alpha directly binds to HNF4alpha-responsive element at the region -202/-177 of the CTRP5 promoter. Taken together, these results show that hepatocyte nuclear factor-4alpha is involved in transcriptional regulation of CTRP5 in the liver.
authors.affiliation: Department of Biochemistry, School of Medicine, Dongguk University, Kyungju, Republic of Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Wan
authors.initials: W
authors.lastname: Lee
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Park
authors.forename: Seung-Yoon
authors.initials: SY
authors.lastname: Park
mesh_terms: D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D005947:Glucose / Q000235:genetics / Q000378:metabolism; D051536:Hepatocyte Nuclear Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators
chemical_list: D051536:Hepatocyte Nuclear Factors; D011506:Proteins; D015534:Trans-Activators; C095901:transcription factor AP-5; D005947:Glucose