title: [Analysis of mutations in DNA polymerase and thymidine kinase genes of herpes simplex virus clinical isolates resistant to antiherpetic drugs].
abstract: The primary structures of DNA-polymerase (ul30) and thymidine kinase (ul23) genes from several herpes simplex virus type 1 (HSV-1) clinical isolates which differed in sensitivity for a number of antiherpetic drugs were determined and compared with those for two laboratory HSV-1 strains one of which was ACV-sensitive (L2), while the another was resistant (L2) to ACV. The phylogenetic analysis of the sequences showed that conserved regions of ul30 gene of HSV-1 clinical isolates and L2 strain were homologous with the exception of point mutations and degenerated substitutions. Several new mutations in the HSV-1 DNA-polymerase and thymidine kinase functional domains were established and identified as the substitutions associated with the strain-resistance to ACV and other drugs.
authors.forename: A N
authors.initials: AN
authors.lastname: Korovina
authors.forename: A A
authors.initials: AA
authors.lastname: Gus'kova
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Skoblov
authors.forename: V L
authors.initials: VL
authors.lastname: Andronova
authors.forename: G A
authors.initials: GA
authors.lastname: Galegov
authors.forename: S N
authors.initials: SN
authors.lastname: Kochetkov
authors.forename: M K
authors.initials: MK
authors.lastname: Kukhanova
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Skoblov
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D024882:Drug Resistance, Viral / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D006561:Herpes Simplex / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017354:Point Mutation; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D013937:Thymidine Kinase; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases; C043417:DNA polymerase, Simplexvirus; D000212:Acyclovir