title: Quantitation of fatty acid ethyl esters in human meconium by an improved liquid chromatography/tandem mass spectrometry.
abstract: This paper reports the development and validation of an improved assay for quantitation of fatty acid ethyl esters (FAEEs) in human meconium using liquid chromatography/tandem mass spectrometry (LC-MS/MS). FAAEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate, ethyl linolenate, and ethyl arachidonate) and the internal standard (I.S.), ethyl heptadecanoate, were separated by reverse phase HPLC and quantified by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the positive ionization mode. The absolute recovery of FAEEs varied from 55+/-10% for 0.33 nmol/g (100 ng/g) of ethyl linoleate up to 86+/-8% for 1.55 nmol/g (500 ng/g) of ethyl miristate. The LODs and LOQs varied from 0.01 to 0.08 nmol/g and from 0.02 to 0.27 nmol/g, respectively. The assay has been successfully applied to examine the FAEE levels in 81 meconium samples from babies born to mothers reporting alcohol consumption, to varying degrees, during pregnancy.
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, Cheil General Hospital and Women's Healthcare Center, Kwandong University School of Medicine, Seoul, Republic of Korea.
authors.forename: Ho-Seok
authors.initials: HS
authors.lastname: Kwak
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kang
authors.forename: Ki-Ok
authors.initials: KO
authors.lastname: Han
authors.forename: Jong-Taek
authors.initials: JT
authors.lastname: Moon
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Chung
authors.forename: June-Seek
authors.initials: JS
authors.lastname: Choi
authors.forename: Jung-Yeol
authors.initials: JY
authors.lastname: Han
authors.forename: Moon-Young
authors.initials: MY
authors.lastname: Kim
authors.forename: E Yadira
authors.initials: EY
authors.lastname: Velázquez-Armenta
authors.forename: Alejandro A
authors.initials: AA
authors.lastname: Nava-Ocampo
mesh_terms: D000437:Alcoholism; D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D008470:Meconium / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004952:Esters; D005227:Fatty Acids