[Validation of the modified algorithm for predicting host susceptibility to viruses taking into account susceptibility parameters of primary target cell cultures and natural immunity factors].
The paper presents results of testing a modified algorithm for predicting virus ID50 values in a host of interest by extrapolation from a model host taking into account immune neutralizing factors and thermal inactivation of the virus. The method was tested for A/Aichi/2/68 influenza virus in SPF Wistar rats, SPF CD-1 mice and conventional ICR mice. Each species was used as a host of interest while the other two served as model hosts. Primary lung and trachea cells and secretory factors of the rats' airway epithelium were used to measure parameters needed for the purpose of prediction. Predicted ID50 values were not significantly different (p = 0.05) from those experimentally measured in vivo. The study was supported by ISTC/DARPA Agreement 450p.
Zhukov
V A
VA
Shishkina
L N
LN
Safatov
A S
AS
Sergeev
A A
AA
P'iankov
O V
OV
Petrishchenko
V A
VA
Zaĭtsev
B N
BN
Toporkov
V S
VS
Sergeev
A N
AN
Nesvizhskiĭ
Iu V
IuV
Vorob'ev
A A
AA
D000465:Algorithms; D000818:Animals; D002478:Cells, Cultured; D004198:Disease Susceptibility; D005260:Female; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D053122:Influenza A Virus, H3N2 Subtype / Q000472:pathogenicity* / Q000502:physiology; D008168:Lung / Q000276:immunology / Q000821:virology; D051379:Mice; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000276:immunology* / Q000821:virology*; D013045:Species Specificity; D014132:Trachea / Q000276:immunology / Q000821:virology