Effect of storage and sodium chloride on excision of CTXPhi or pre-CTXPhi and CTXPhi from Vibrio cholerae O139 strains.
We examined the effect of storage and sodium chloride on excision of CTXPhi or pre-CTXPhi and CTXPhi from Vibrio cholerae O139 strains. We found that one strain of V. cholerae O139 VO146P showed loss of the complete phage array, and other strain VO170P showed partial loss of the phage array giving rise to altered strains designated as VO146N and VO170N. Results of PCR and RFLP analysis revealed that both strains (VO146P and VO170P) possessed a single copy of pre-CTX(ET)Phi and two copies of CTXPhi comprising CTX(Class)Phi and CTX(Calc)Phi arranged in tandem, and integrated in the large chromosome. The presence of classical ctxB was detected in CTX(Calc)Phi of both V. cholerae O139 strains. Nucleotide sequencing of three housekeeping genes showed no difference between parent and altered strains of V. cholerae O139.
Mantri
Chinmay K
CK
Infectious Disease Biology, Institute of Life Sciences, Nalco Square, Bhubaneswar, Orrisa 751023, India.
Mohapatra
Saswat S
SS
Singh
Durg V
DV
D002772:Cholera Toxin / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D014644:Genetic Variation; D016680:Genome, Bacterial; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D011309:Preservation, Biological / Q000379:methods; D039002:Prophages / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors; D041242:Vibrio cholerae O139 / Q000145:classification / Q000187:drug effects / Q000821:virology*; D014775:Virus Activation / Q000187:drug effects / Q000502:physiology*
D004279:DNA, Viral; D012965:Sodium Chloride; D002772:Cholera Toxin