title: MEKC determination of IgG in human serum via a pH-mediated acid stacking method.
abstract: An on-column preconcentration technique, pH-mediated acid stacking, was used in this study to improve the sensitivity of MEKC-UV analysis of IgG in human serum. Various parameters affecting pH-mediated acid stacking were optimized systematically. To eliminate the matrix interferences of human serum and to combine the sample pretreatment procedure with the detection methodology, silica-coated Fe(3)O(4) magnetic nanoparticles modified with N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane were prepared and employed as solid phase extraction adsorbent to remove the abundant HSA from human serum. HSA was quantitatively removed by silica-coated Fe(3)O(4) magnetic nanoparticles modified with N-(2-aminoethyl)-3-aminopropyl-trimethoxysilanes without retaining IgG at pH 9.3. Under the optimum conditions, the sensitivity of IgG was improved 40.3-fold using a 100 s electrokinetic injection as compared with a 6 s hydrodynamic injection. The detection limit of IgG was found to be 0.1 mg/L, and the proposed method was successfully applied for the determination of IgG in human serum with satisfactory results.
authors.affiliation: Department of Chemistry and Environmental Engineering, Hubei Normal University, Huangshi, PR China. chemwuyiwei@tom.com
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Wu
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Liu
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Deng
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.forename: Kun
authors.initials: K
authors.lastname: Xiong
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D005290:Ferric Compounds; D007074:Immunoglobulin G; C000499:ferric oxide