title: Antioxidant and free radical-scavenging activity of the extracellular death factor in Escherichia coli.
abstract: The extracellular death factor (EDF) is a newly discovered linear pentapeptide with the sequence of H-Asn-Asn-Trp-Asn-Asn-OH (NNWNN). It could act as a quorum-sensing molecule and participate in the mazEF-mediated cell death in Escherichia coli. In the present study, we firstly studied the antioxidant and free radical-scavenging activities of EDF. EDF could scavenge hydroxyl radicals in vitro and protect protein, lipid and DNA from being damaged by hydroxyl radicals. Our results indicated that this extracellular death factor might have dual effects during the programmed cell death process of Escherichia coli.
authors.affiliation: Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China. gaoyf@zzu.edu.cn
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Gao
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Li
authors.forename: Xiangjiao
authors.initials: X
authors.lastname: Jia
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Lei
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Yan
authors.forename: Liangliang
authors.initials: L
authors.lastname: Kong
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D004249:DNA Damage; D004926:Escherichia coli / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D053038:Quorum Sensing; D013481:Superoxides / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D009842:Oligopeptides; D017392:Thiobarbituric Acid Reactive Substances; C528802:asparagyl-aspargyl-tryptophyl-asparagyl-asparagine; D013481:Superoxides; D017665:Hydroxyl Radical