Lack of effect of intranigral transplants of a GABAergic cell line on absence seizures.
The substantia nigra pars reticulata (SNpr) is involved in controlling a variety of seizure phenomena. Intranigral transplants of GABAergic cells have been shown to decrease the severity of already established epileptic seizures, but the effects observed have been short-lived. This study evaluated the ability of intranigral transplants of GABA-producing cells to reduce spontaneous absence seizures in a genetic animal model for periods up to 3 months after transplantation. Intranigral transplants did not induce any behavioral deficits in the animals, and they did not form tumors; however, the transplants failed to decrease absence seizures in the genetic model. The assumed increase in intranigral levels of GABA after the transplants may be insufficient to counteract all the factors involved in generating the absence seizures; in this animal model, it may be necessary to further decrease nigral activity by implanting GABAergic cells in another area. These results bear down on the fact that cell transplants need to be tailored for each type of convulsive disorder in terms of the type of cells delivered and the location of the transplants.
Castillo
Claudia G
CG
Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, México.
Mendoza
Soledad
S
Saavedra
Josie
J
Giordano
Magda
M
D000818:Animals; D001835:Body Weight; D002460:Cell Line; D017690:Cell Transplantation / Q000502:physiology*; D004195:Disease Models, Animal; D004832:Epilepsy, Absence / Q000503:physiopathology / Q000601:surgery*; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D008124:Locomotion / Q000502:physiology; D008297:Male; D009474:Neurons / Q000378:metabolism; D011597:Psychomotor Performance / Q000502:physiology; D051381:Rats; D013378:Substantia Nigra / Q000378:metabolism / Q000473:pathology; D014162:Transfection / Q000379:methods; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1