Atractylodes japonica Koidzumi inhibits the production of proinflammatory cytokines through inhibition of the NF-kappaB/IkappaB signal pathway in HMC-1 human mast cells.
The rhizome of Atractylodes japonica Koidzumi (AJK) has been used in traditional medicine for treatment of arthritis, bronchitis and respiratory infectious disease, whereas its effects on inflammatory reactions have not been unknown recently. In this study, the effects of AJK on allergic inflammation and its signaling were investigated in the induced human mast cells and animal model. This study showed that ethanol extract of AJK interestingly suppressed the production and mRNA expression of TNF-alpha, IL-6 and IL-8, as important inflammatory cytokines. Furthermore, AJK inhibited the nuclear translocation of nuclear factor (NF)-kappaB through inhibition of the phosphorylation of IB-kappa, which was additionally elucidated by NF-kappaB promoter-mediated luciferase activity. In addition, the phosphorylation of ERK was increased in pretreatment with AJK, whereas there was no change in JNK and p38 MAPK. However, AJK showed no effects on anti-DNP IgE-mediated in vivo PCA reaction and histamine release, as key events of mast cell-mediated immediate allergic reactions. These results suggest that AJK might be involved in not early-phase but transition to late-phase reactions of allergic inflammation and could modulate through other signal pathways. Taken together, AJK could be used as a treatment for mast cell mediated late-phase/chronic allergic inflammatory reactions.
Hong
Myung Hee
MH
Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University, Seoul, Korea.
Kim
Jeong-Hyun
JH
Bae
Hyunsu
H
Lee
Na-Youn
NY
Shin
Yong-Cheol
YC
Kim
Sung-Hoon
SH
Ko
Seong-Gyu
SG
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D031174:Atractylodes / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism*; D007783:Lactones / Q000032:analysis; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008518:Medicine, East Asian Traditional; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D020672:I-kappa B Proteins; D007783:Lactones; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D012717:Sesquiterpenes; C424802:atractylenolide III