A strategy for direct mapping and identification of mutations by whole-genome sequencing.
Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.
Zuryn
Steven
S
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Institut National de Santé et de Recherche Médicale (INSERM) U964/Centre National de Recherche Scientifique (CNRS) UMR 1704/Université de Strasbourg, 67404 Illkirch CU Strasbourg, France.
Le Gras
Stéphanie
S
Jamet
Karine
K
Jarriault
Sophie
S
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D016296:Mutagenesis; D009154:Mutation