Simultaneous HPLC determination of three bioactive alkaloids in the Asian medicinal plant Stephania rotunda.
A reliable high-performance liquid chromatography (HPLC) method coupled with photodiode array detection has been developed and validated for the determination of three major alkaloids: cepharanthine, tetrahydropalmatine and xylopinine in Stephania rotunda Lour. (Menispermaceae) collected in Cambodia. The chromatographic separation was carried out on a Symmetry C8 column (250 mm x 4.6 mm, 5 microm, Waters), with an isocratic solvent system of 25 mM potassium phosphate buffer (pH 3.5) - acetonitrile. UV detection was performed at 282 nm. Good linear behavior over the investigated concentration ranges was observed with values of r2 > 0.9964 for all the analytes. The method was reproducible with intra- and inter-day variations of less than 3.91%. The mean recoveries of the analytes ranged from 95.7 to 104.6%. The proposed method was linear, accurate, precise and specific. The validated method was successfully applied to quantify the three alkaloids in various parts of Stephania rotunda and in tubers collected from different Cambodian regions. The results indicated that the developed HPLC method could be used for the quality control of S. rotunda.
Bory
Sothavireak
S
Laboratory of Pharmacognosy and Ethnopharmacology, UMR-MD3, Faculty of Pharmacy, University of the Mediterranean Aix-Marseille II, 27 Bad Jean Moulin, F-13385 Marseille cedex 5, France.
Bun
Sok-Siya
SS
Baghdikian
Béatrice
B
Mabrouki
Fathi
F
Cheng
Sun Kaing
SK
Elias
Riad
R
Bune
Hot
H
Ollivier
Evelyne
E
D044182:Benzylisoquinolines / Q000737:chemistry*; D001600:Berberine Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D031607:Stephania / Q000737:chemistry*; D052117:Benzodioxoles
D044182:Benzylisoquinolines; D001600:Berberine Alkaloids; C453820:xylopinine; C014215:tetrahydropalmatine; C006947:cepharanthine; D052117:Benzodioxoles