[Detection of platelet-activating factor in unstimulated mixed saliva and gingival crevicular fluid of periodontitis patients with coronary heart disease].
OBJECTIVE
To explore the correlation between periodontitis and coronary heart diseases(CHD) by comparing the levels of platelet-activating factor (PAF) in saliva and gingival crevicular fluid (GCF) among 4 groups.


METHODS
One hundred and twenty subjects were divided into 4 groups according to the diagnostic angiography and periodontal examination as group (C+P), group C, group P and group H.Group (C+P) was made up of 24 CHD patients with moderate to advanced periodontitis.36 CHD patients without periodontitis were as group C.Group P was made up of 32 patients suffering moderate to advanced periodontitis.Group H was 28 healthy volunteers. The general information and periodontal status of all the subjects were recorded and unstimulated mixed saliva and GCF samples were collected.The PAF level was detected by ELISA. Then the difference in PAF level of each group was analyzed with SPSS13.0 software package.


RESULTS
One way analysis of variance showed that the levels of PAF in saliva and GCF from the patients of group (C+P) and P were significantly higher than the group C and group H. The results of Pearson correlation analysis showed that the levels of PAF in saliva and GCF positively correlated with pocket depth (PD) and attachment loss (AL) (P<0.05).


CONCLUSIONS
Inflammation of periodontal tissues can increase the level of PAF which may be a risk factor of coronary heart diseases. It indicates that the raising level of PAF may be another significant factor in the process of periodontitis affecting the progress of coronary heart disease.
Feng
Wei
W
Department of Periodontics, Stomatological Hospital of Shandong University, The Key Laboratory of Oral Biomedicine of Shandong Province, Jinan 250012, China. maomaochong1003@163.com
Wu
Ting
T
Sun
Qin-feng
QF
Yang
Pi-shan
PS
Liu
Xiao-ling
XL
D000368:Aged; D001792:Blood Platelets; D003327:Coronary Disease; D005883:Gingival Crevicular Fluid; D006801:Humans; D010514:Periodontal Pocket; D010518:Periodontitis; D010972:Platelet Activating Factor; D012307:Risk Factors; D012463:Saliva
D010972:Platelet Activating Factor