title: Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
abstract: The in vivo immunoadjuvant effects of CpG oligodeoxynucleotides (CpG-ODN) have been studied extensively in mice and relatively fewer studies have been done in piglets. But so far, the innate immunostimulatory effects of CpG-ODN combination with innate defense-regulator peptides (IDRs) have not been demonstrated. The purpose of this study is to determine the potential effects of CpG-ODN with IDR in newborn piglets. The immunostimulatory abilities of four selected IDRs were compared, among them HH2 showed best immunostimulatory effects in newborn piglets. Hereafter, the abilities of CpG-ODN combined with HH2 to enhance innate immune responses were examined in newborn piglets. The complex of HH2 and CpG-ODN could induce much stronger Th1 cytokine and chemokine responses than HH2 or CpG-ODN alone. HH2-CpG-ODN immunized piglets showed higher B cell percentage in PBMCs than CpG-ODN alone. These in vivo data demonstrated for the first time that subcutaneously (SC) administration of CpG-ODN combined with HH2 is efficient to stimulate innate immune system in newborn piglets.
authors.affiliation: College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Cao
authors.forename: Huazhou
authors.initials: H
authors.lastname: Li
authors.forename: Zhenggu
authors.initials: Z
authors.lastname: Jiang
authors.forename: Chenchao
authors.initials: C
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Cheng
authors.forename: Zhaihan
authors.initials: Z
authors.lastname: Yang
authors.forename: Guangjun
authors.initials: G
authors.lastname: Cao
authors.forename: Linghua
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000097:blood / Q000276:immunology; D007113:Immunity, Innate; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology* / Q000494:pharmacology; D013552:Swine
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D016207:Cytokines; D009838:Oligodeoxyribonucleotides