[Characterization of stem cells derived from the neonatal auditory sensory epithelium].
BACKGROUND
In contrast to regenerating hair cell-bearing organs of nonmammalian vertebrates the adult mammalian organ of Corti appears to have lost its ability to maintain stem cells. The result is a lack of regenerative ability and irreversible hearing loss following auditory hair cell death. Unexpectedly, the neonatal auditory sensory epithelium has recently been shown to harbor cells with stem cell features. The origin of these cells within the cochlea's sensory epithelium is unknown.


METHODS
We applied a modified neurosphere assay to identify stem cells within distinct subregions of the neonatal mouse auditory sensory epithelium. Sphere cells were characterized by multiple markers and morphologic techniques.


RESULTS
Our data reveal that both the greater and the lesser epithelial ridge contribute to the sphere-forming stem cell population derived from the auditory sensory epithelium. These self-renewing sphere cells express a variety of markers for neural and otic progenitor cells and mature inner ear cell types.


CONCLUSIONS
Stem cells can be isolated from specific regions of the auditory sensory epithelium. The distinct features of these cells imply a potential application in the development of a cell replacement therapy to regenerate the damaged sensory epithelium.
Diensthuber
M
M
Klinik für Hals-Nasen-Ohren-Heilkunde, Kopf- und Halschirurgie, Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Deutschland. Marc.Diensthuber@kgu.de
Heller
S
S
D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000166:cytology*; D004848:Epithelium / Q000033:anatomy & histology; D006198:Hair Cells, Auditory / Q000166:cytology*; D007760:Labyrinth Supporting Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000166:cytology*