title: Comparative study on the anti-inflammatory and immune suppressive effect of Wilforlide A.
abstract: Tripterygium Glycosides (TG) is effective in the treatment of patients with a variety of inflammatory and autoimmune diseases, especially rheumatoid arthritis (RA) in clinical. Wilforlide A (T(1)) serves as a quality control standard of TG that be listed in Drug Standard of Ministry of Public Health of the People's Republic of China. The pharmacologic actions of T(1) remain to be unidentified. In this paper, we studied the anti-inflammatory and immune suppressive effect of T(1), and compared it with Triptolide (TP), which believed to be the major active component of TG. Carrageenan-induced rat pedal swelling, tampon-induced rat granulation, and mice ear inhibition rate of swelling trail results show that high-dose T(1) has obvious anti-inflammatory effect. Colorimetric detection contents of hemolysin, carbon elimination from plasma of mice, mice delayed hypersensitivity immune, and organ index were measured. The results show that T(1) has no significant immune suppressive activity, and there has a significant difference with TP and TG. Therefore, we think the content of TP also should be controlled as a supplement standard in order to ensure safe and effective medication.
authors.affiliation: Jiangsu Center for Drug Screening Laboratory, China Pharmaceutical University No.24, Tongjiaxiang, Nanjing, Jiangsu Province 210009, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Zhen-zhou
authors.initials: ZZ
authors.lastname: Jiang
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Liu
authors.forename: Lu-yong
authors.initials: LY
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Zhi-xing
authors.initials: ZX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000737:chemistry; D014992:Xylenes / Q000633:toxicity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007166:Immunosuppressive Agents; D014992:Xylenes; D009828:Oleanolic Acid; C052981:wilforlide A; D002351:Carrageenan