title: [Variation of endogenous hormones in formation of microtubers of Pinellia ternata in vitro].
abstract: OBJECTIVE
  Through analysis of variation and function of five endogenous hormones (IAA, GA3, ABA, ZR and JA) in the formation of microtubers of Pinellia ternata in vitro to explore the physiological and biochemical mechanism of tubers' development.
  
  
  METHODS
  The endogenous hormones of microtubers were isolated in different stages of tubers expansion when the leaf stalks of P. ternata were cultured on MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 5% medium to induce the formation of microtubers, then them and stems were detected with Enzyme-linked Immunosorbent Assays (ELISA).
  
  
  RESULTS
  The results showed that the content of IAA, ABA, JA and ZR in the intumescentia period of microtubers were rapidly rising. The curve of GA3 appeared the type of anti-S.
  
  
  CONCLUSIONS
  IAA, ABA, JA, ZR and GA3 played an important role in controlling formation of microtubers in P. ternata.
authors.affiliation: School of Life Science, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Jianping
authors.initials: J
authors.lastname: Xue
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Chang
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Fan
mesh_terms: D000704:Analysis of Variance; D006728:Hormones / Q000378:metabolism*; D030021:Pinellia / Q000254:growth & development / Q000378:metabolism*; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006728:Hormones