title: An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells.
abstract: To reduce the interference in MTT reduction assay, we developed an improved protocol for evaluating the viability of Escherichia coli cells by conducting MTT reduction in 1.5-mL centrifuge tubes and formazan dissolution in test tubes. Our study is helpful in developing protocols for measuring the viability of other gram-negative bacteria.
authors.affiliation: Newworld Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Hengwei
authors.initials: H
authors.lastname: Wang
authors.forename: Hairong
authors.initials: H
authors.lastname: Cheng
authors.forename: Fengqing
authors.initials: F
authors.lastname: Wang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
authors.forename: Xuedong
authors.initials: X
authors.lastname: Wang
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue