Effects of four Bifidobacteria on obesity in high-fat diet induced rats.
OBJECTIVE
To compare the effects of four Bifidobacteria strains (Bifidobacteria L66-5, L75-4, M13-4 and FS31-12, originated from normal human intestines) on weight gain, lipid metabolism, glucose metabolism in an obese murine model induced by high-fat diet.


METHODS
Forty-eight Sprague-Dawley rats were randomly divided into six groups. Control group received standard chow, model group received high-fat diet, and intervention groups received high-fat diet added with different Bifidobacteria strains isolated from healthy volunteers' fresh feces. All rats were executed at the 6th weekend. Body weight (BW), obese indexes, oral glucose tolerance test, serum and liver lipid and serum insulin (INS) were tested. Liver lipid deposition was classified pathologically.


RESULTS
Compared with the model group, B. M13-4 improved BW gains (264.27 +/- 26.91 vs 212.55 +/- 18.54, P = 0.001) while B. L66-5 induced a decrease in BW (188.47 +/- 11.96 vs 212.55 +/- 18.54, P = 0.043). The rest two strains had no significant change in BW. All the four strains can reduce serum and liver triglyceride and significantly alleviate the lipid deposition in liver. All strains showed a trend of lowing serum and liver total cholesterol while B. L66-5 and B. FS31-12 did so more significantly. In addition, all the four strains showed no significant differences in serum INS and glucose level.


CONCLUSIONS
The response of energy metabolism to administration of Bifidobacteria is strain dependent. Different strains of Bifidobacteria might drive different directions of fat distribution.
Yin
Ya-Ni
YN
Department of Gastroenterology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
Yu
Qiong-Fen
QF
Fu
Nian
N
Liu
Xiao-Wei
XW
Lu
Fang-Gen
FG
D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004032:Diet; D004041:Dietary Fats; D004195:Disease Models, Animal; D005951:Glucose Tolerance Test; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
D001786:Blood Glucose; D004041:Dietary Fats; D008055:Lipids; D014280:Triglycerides; D002784:Cholesterol