The epitope of the VP1 protein of porcine parvovirus.
Porcine parvovirus (PPV) is the major causative agent in a syndrome of reproductive failure in swine. Much has been learned about the structure and function of PPV in recent years, but nothing is known about the epitopes of the structural protein VP1, which is an important antigen of PPV. In this study, the monoclonal antibody C4 against VP1 of PPV was prepared and was used to biopan a 12-mer phage peptide library three times. The selected phage clones were identified by ELISA and then sequencing. The amino acid sequences detected by phage display were analyzed, and a mimic immuno-dominant epitope was identified. The epitope of VP1 is located in the N-terminal and contains the role amino acid sequence R-K-R. Immunization of mice indicated that the phage-displayed peptide induces antibodies against PPV. This study shows that peptide mimotopes have potential as alternatives to the complex antigens currently used for diagnosis of PPV infection or for development of vaccines.
Xie
Hong-ling
HL
Division of Swine Infection Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Wang
Zhao
Z
Cui
Shang-jin
SJ
Zhang
Chao-fan
CF
Cui
Yu-dong
YD
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018604:Epitope Mapping; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D028323:Parvovirus, Porcine / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology*; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D019151:Peptide Library; D015678:Viral Structural Proteins