Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina.
The polysaccharide (PAP) from Potentilla anserina was evaluated for modulating effects by using mouse peritoneal macrophage and the immunosuppressed-model cyclophosphamide-induced. Phagocytotic and mononuclear phagocytic system function assays showed that PAP stimulated the phagocytosis of phagocyte. Splenocyte proliferation assay showed that PAP acted the effect combining Con A or LPS in splenocyte proliferation. The parameters detected showed that PAP increased thymus and spleen indices, the levels of LDH and ACP in the spleen, and IL-10 and IFN-γ in serum in immunosuppressed mice. The results suggest that PAP is involved in immunomodulatory effects leading to the exploration for PAP as a potential immunostimulant.
Chen
Jiong-Ran
JR
Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, No. 335 Jiangouyan, Xiaoxihu, Lanzhou, Gansu 730050, PR China.
Yang
Zhi-Qiang
ZQ
Hu
Ting-Jun
TJ
Yan
Zuo-Ting
ZT
Niu
Ting-Xian
TX
Wang
Ling
L
Cui
Dong-An
DA
Wang
Meng
M
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D056747:Immunomodulation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D031987:Potentilla / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects
D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase