Review: role of tubal environment in preimplantation embryogenesis: application to co-culture assays.
The culture of early preimplantation stage embryo is still delicate and the metabolic pathways of embryos are not completely understood. Embryo needs are evolutionary during the preimplantation development, consequently it is difficult to meet embryo needs in vitro. Culture conditions have to respect several physical and chemical equilibria: such as redox potential, pH, osmotic pressure, metabolic flux of energetic compounds, endogenous pools of amino acids and transcripts, etc. Embryo culture media are generally supplemented with amino acids, glucose, other energetic metabolites and antioxidant compounds, vitamin, and growth factors etc. Furthermore autocrine and paracrine regulation of embryo development probably exist. In fact embryo culture conditions have to be as non-toxic as possible. Various types of co-culture systems have been devised to overcome these problems. Complex interrelations exist between embryos and co-cultured cells. The beneficial effects of co-cultured cells may be due to continuous modifications of the culture medium, i.e. the elimination of toxic compounds and/or the supply of embryotrophic factors.
Guérin
Pierre
P
p.guerin@vet-lyon.fr
Ménézo
Yves
Y
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D018920:Coculture Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D009997:Osmotic Pressure
D000596:Amino Acids; D003470:Culture Media