title: Inhibition of mitochondrial function reduces DNA repair in human mononuclear cells.
abstract: BACKGROUND
  Mitochondria provide ATP and Ca(2+) needed for DNA repair, but also produce reactive oxygen species (ROS), which may damage DNA.
  
  
  OBJECTIVE
  To investigate the effect of mitochondrial function inhibition on DNA repair.
  
  
  METHODS
  Five mitochondrial inhibitors acting at various sites of electron transport were studied. Human peripheral blood mononuclear cells, spontaneous and H(2)O(2)-induced DNA repair, as well as %-double-stranded-DNA, were measured.
  
  
  RESULTS
  All mitochondrial inhibitors suppressed spontaneous and H(2)O(2)-induced DNA repair. However, their effect on %-double-stranded-DNA differed, which is partly related to ROS suppression.
  
  
  CONCLUSIONS
  Mitochondrial inhibition may enhance efficacy and reduce toxicity of radiation and cytotoxic drugs therapy.
authors.affiliation: Department of Hematology, Rabin Medical Center, Petah Tikva, Israel.
authors.forename: Anat
authors.initials: A
authors.lastname: Gafter-Gvili
authors.forename: Michal
authors.initials: M
authors.lastname: Herman
authors.forename: Yaacov
authors.initials: Y
authors.lastname: Ori
authors.forename: Asher
authors.initials: A
authors.lastname: Korzets
authors.forename: Avry
authors.initials: A
authors.lastname: Chagnac
authors.forename: Boris
authors.initials: B
authors.lastname: Zingerman
authors.forename: Benaya
authors.initials: B
authors.lastname: Rozen-Zvi
authors.forename: Uzi
authors.initials: U
authors.lastname: Gafter
authors.forename: Tsipora
authors.initials: T
authors.lastname: Malachi
mesh_terms: D004260:DNA Repair / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D017382:Reactive Oxygen Species; D014475:Uncoupling Agents