title: DHA-1-producing Klebsiella pneumoniae in a teaching hospital in the Czech Republic.
abstract: Thirty-eight AmpC-producing Klebsiella pneumoniae isolates identified from January to October 2006 in a large teaching hospital in the Czech Republic were analyzed. The AmpC cephalosporinase was identified as DHA-1, encoded by a plasmid-located complex class 1 integron, previously observed in a K. pneumoniae isolate from the Parisian region. The DHA-1 expression was inducible, and although in two isolates with higher resistance, the induction effect was masked at the phenotypic level. All of the isolates belonged to the international K. pneumoniae clone sequence type 11, split into two disseminated pulsed-field gel electrophoresis types. This is the first report on enterobacteriaceae with acquired AmpCs in the Czech Republic and possibly the first description of organisms with DHA-1 in the Central and Eastern Europe.
authors.affiliation: National Medicines Institute, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Empel
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Hrabák
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kozińska
authors.forename: Tamara
authors.initials: T
authors.lastname: Bergerová
authors.forename: Pavla
authors.initials: P
authors.lastname: Urbášková
authors.forename: Izabela
authors.initials: I
authors.lastname: Kern-Zdanowicz
authors.forename: Marek
authors.initials: M
authors.lastname: Gniadkowski
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D018153:Czech Republic / Q000453:epidemiology; D016521:Electrophoresis, Gel, Pulsed-Field; D006784:Hospitals, Teaching; D006801:Humans; D032023:Integrons / Q000235:genetics; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases