[Effects of simvastatin on proliferation and transition of phenotype in PDGF-BB-induced pulmonary artery smooth muscle cells].
OBJECTIVE
To investigate the effects of simvastatin on inhibiting cell phenotype and potential mechanism in PASMC induced by PDGF-BB.


METHODS
PASMC isolated from SD rats and cultured in vitro were induced by PDGF-BB and then intervened by simvastatin and Y-27632. Flow cytometry were performed to detect cell proliferation. Fluorescence quantitative RT-PCR was performed to detect alpha-SM-actin mRNA expression and Western blot was applied to detect the expression of alpha-SM-actin, SM22alpha and RhoA protein.


RESULTS
Simvastatin and Y-27632 obviously increased the level of mRNA of alpha-SM-actin and promoted the protein expression of alpha-SM-actin, SM22alpha while inhibiting the proliferation of PASMC induced by PDGF-BB. Simultaneously, inhibited expression of RhoA induced by simvastatin and Y-27632 was also observed.


CONCLUSIONS
The inhibited effect of simvastatin on the proliferation of PASMC induced by PDGF-BB may be achieved by inhibiting conversion of cell phenotype. And the potential mechanism may through the RhoA/Rho kinase signaling pathway.
Wang
Xiao-Qin
XQ
Department of Padiatric Cardiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Tong-Fu
TF
Liu
Han-Min
HM
Wang
Juan
J
Wang
Xian-Min
XM
Liu
Bin
B
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D000818:Animals; D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D011651:Pulmonary Artery / Q000166:cytology*; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019821:Simvastatin / Q000494:pharmacology*; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*
D000199:Actins; D000577:Amides; D000077214:Becaplermin; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011725:Pyridines; D012333:RNA, Messenger; D019821:Simvastatin; D020742:rhoA GTP-Binding Protein; C498862:Acta2 protein, rat; C108830:Y 27632