title: C677T polymorphism of the 5,10 MTHFR gene in young Mexican subjects with ST-elevation myocardial infarction.
abstract: OBJECTIVE
  The C677T polymorphism of 5,10 methylenetetrahydrofolate reductase (MTHFR) gene has been associated with hypertension and coronary artery disease in several populations worldwide, but results are still controversial. The aim of this study was to examine the possible association of C677T polymorphism with ST-elevation myocardial infarction (STEMI) in young Mexican subjects.
  
  
  METHODS
  In a case-control study, 167 unrelated patients < or = 45 years of age with diagnosis of STEMI who were admitted to a cardiovascular intense care unit and 167 unrelated controls subjects matched by age and gender were recruited from January 2006 and June 2009. The C677T polymorphism was determined in all participants by a polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP).
  
  
  RESULTS
  There was no significant difference in the genotype distribution between groups (p = 0.69) or allele frequency (p = 0.40). There were independent factors for STEMI: smoking (OR 4.9, 95% CI 3.0-8.1, p = 0.001), hypertension (OR 1.8, 95% CI 1.0-3.3, p = 0.03), family history of atherothrombotic disease (OR 2.3, 95% CI 2.0-4.6, p = 0.02), and dyslipidemia (OR 3.2, 95% CI 1.8-5.6, p <0.001). Diabetes mellitus did not represent an independent risk factor for STEMI (OR 1.2, 95% CI 0.2-2.2, p = 0.82).
  
  
  CONCLUSIONS
  The TT genotype from the C677T of 5,10 MTHFR gene is not an independent risk factor for STEMI in the Mexican population. However, more studies are needed to determine the possible "protective effect" of the C677T polymorphism in our population.
authors.affiliation: Unidad de Investigación Médica en Trombosis, Hemostasia y Aterogénesis, H.G.R. No. 1, Dr. Carlos MacGregor Sánchez Navarro, Instituto Mexicano del Seguro Social, México, D.F., México. irmaisordia@yahoo.com.mx
authors.forename: Irma
authors.initials: I
authors.lastname: Isordia-Salas
authors.forename: Adriana
authors.initials: A
authors.lastname: Trejo-Aguilar
authors.forename: M Guadalupe
authors.initials: MG
authors.lastname: Valadés-Mejía
authors.forename: David
authors.initials: D
authors.lastname: Santiago-Germán
authors.forename: Alfredo
authors.initials: A
authors.lastname: Leaños-Miranda
authors.forename: Lorena
authors.initials: L
authors.lastname: Mendoza-Valdéz
authors.forename: Ricardo
authors.initials: R
authors.lastname: Jáuregui-Aguilar
authors.forename: Gabriela
authors.initials: G
authors.lastname: Borrayo-Sánchez
authors.forename: Abraham
authors.initials: A
authors.lastname: Majluf-Cruz
mesh_terms: D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008800:Mexico; D009203:Myocardial Infarction / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; D042965:Methylenetetrahydrofolate Reductase (NADPH2)