Comparative study on the anti-inflammatory and immune suppressive effect of Wilforlide A.
Tripterygium Glycosides (TG) is effective in the treatment of patients with a variety of inflammatory and autoimmune diseases, especially rheumatoid arthritis (RA) in clinical. Wilforlide A (T(1)) serves as a quality control standard of TG that be listed in Drug Standard of Ministry of Public Health of the People's Republic of China. The pharmacologic actions of T(1) remain to be unidentified. In this paper, we studied the anti-inflammatory and immune suppressive effect of T(1), and compared it with Triptolide (TP), which believed to be the major active component of TG. Carrageenan-induced rat pedal swelling, tampon-induced rat granulation, and mice ear inhibition rate of swelling trail results show that high-dose T(1) has obvious anti-inflammatory effect. Colorimetric detection contents of hemolysin, carbon elimination from plasma of mice, mice delayed hypersensitivity immune, and organ index were measured. The results show that T(1) has no significant immune suppressive activity, and there has a significant difference with TP and TG. Therefore, we think the content of TP also should be controlled as a supplement standard in order to ensure safe and effective medication.
Xue
Mei
M
Jiangsu Center for Drug Screening Laboratory, China Pharmaceutical University No.24, Tongjiaxiang, Nanjing, Jiangsu Province 210009, China.
Jiang
Zhen-zhou
ZZ
Liu
Ji-ping
JP
Zhang
Lu-yong
LY
Wang
Tao
T
Wang
Hao
H
Liu
Li
L
Zhou
Zhi-xing
ZX
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000737:chemistry; D014992:Xylenes / Q000633:toxicity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007166:Immunosuppressive Agents; D014992:Xylenes; D009828:Oleanolic Acid; C052981:wilforlide A; D002351:Carrageenan