title: Nicotinic acetylcholine receptor activation reduces skeletal muscle inflammation of mdx mice.
abstract: Mdx mice develop an inflammatory myopathy characterized at different ages by myonecrosis with scattered inflammatory infiltrates followed by muscular regeneration and later persistent fibrosis. This work aimed to verify the putative anti-inflammatory role of nicotinic acetylcholine receptor (nAChR) in the mdx muscular lesion. Mitigation of myonecrosis and decreased TNFα production were accompanied by increased numbers of F4/80 macrophages expressing nAChRα7. In vivo treatment with nicotine attenuated muscular inflammation characterized by reduced metalloprotease MMP-9 activity, TNFα and NFkB content and increased muscular regeneration. Our data indicate that nAChR activation influences local inflammatory responses in the muscular lesion of mdx mice.
authors.affiliation: Laboratório de Patologia Celular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Paulo Emílio Corrêa
authors.initials: PE
authors.lastname: Leite
authors.forename: Jussara
authors.initials: J
authors.lastname: Lagrota-Candido
authors.forename: Louise
authors.initials: L
authors.lastname: Moraes
authors.forename: Livia
authors.initials: L
authors.lastname: D'Elia
authors.forename: Douglas Florindo
authors.initials: DF
authors.lastname: Pinheiro
authors.forename: Rafael Ferreira
authors.initials: RF
authors.lastname: da Silva
authors.forename: Edna N
authors.initials: EN
authors.lastname: Yamasaki
authors.forename: Thereza
authors.initials: T
authors.lastname: Quirico-Santos
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D020388:Muscular Dystrophy, Duchenne / Q000276:immunology / Q000473:pathology / Q000628:therapy; D009220:Myositis / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D009336:Necrosis; D021122:Protein Subunits / Q000378:metabolism / Q000502:physiology; D011978:Receptors, Nicotinic / Q000378:metabolism* / Q000502:physiology; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: C579166:Chrna7 protein, mouse; D018836:Inflammation Mediators; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor