title: Detection of six genetically modified maize lines using optical thin-film biosensor chips.
abstract: As more and more genetically modified organisms (GMO) are commercialized, efficient and inexpensive assays are required for their quick detection. An event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. This study developed a system for detecting six GM maize lines (Bt11, Bt176, GA21, MON810, NK603, and T25) using optical silicon thin-film biosensor chips. Aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface. Biotinylated PCR amplicons were then hybridized with the probes. After washing and brief incubation with an anti-biotin IgG horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated PCR amplicons perfectly matched with the probes can be visualized by the color change on the chip surface (gold to blue/purple). This assay is extremely robust, exhibits high sensitivity and specificity, and is flexible from low through moderate to high throughput.
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.
authors.forename: Sulan
authors.initials: S
authors.lastname: Bai
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Shucheng
authors.initials: S
authors.lastname: Li
authors.forename: Haodong
authors.initials: H
authors.lastname: Chen
authors.forename: William
authors.initials: W
authors.lastname: Terzaghi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiurong
authors.initials: X
authors.lastname: Chi
authors.forename: Jin
authors.initials: J
authors.lastname: Tian
authors.forename: Hongxia
authors.initials: H
authors.lastname: Luo
authors.forename: Wensheng
authors.initials: W
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Yaochuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*