title: Elimination of carbamazepine by repeated treatment with laccase in the presence of 1-hydroxybenzotriazole.
abstract: Carbamazepine (CBZP) is used as an antiepileptic drug and is highly persistent. In this study, CBZP was treated with laccase from white rot fungus Trametes versicolor in the presence of a redox mediator 1-hydroxybenzotriazole (HBT). A single treatment with laccase and HBT eliminated CBZP by about 22% after 24h, and repeated treatments with laccase and HBT, which were added to the reaction mixture every 8h, helped eliminate about 60% of CBZP after 48h. This suggests that repeated treatment is effective in eliminating CBZP. Mass spectrometric analyses demonstrated that two degradation products of CBZP, 10,11-dihydro-10,11-epoxycarbamazepine and 9(10H)-acridone, were formed via repeated treatment with laccase and HBT.
authors.affiliation: Faculty of Agriculture, Shizuoka University, Surugaku, Shizuoka, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hata
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shintate
authors.forename: Shingo
authors.initials: S
authors.lastname: Kawai
authors.forename: Hideo
authors.initials: H
authors.lastname: Okamura
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000927:Anticonvulsants / Q000378:metabolism; D002220:Carbamazepine / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D055454:Trametes / Q000201:enzymology; D014230:Triazoles
chemical_list: D000927:Anticonvulsants; D014230:Triazoles; D002220:Carbamazepine; C011852:1-hydroxybenzotriazole; D042845:Laccase