Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.
Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound, doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.
Andersson
Henrik
H
Division of Biotechnology/IFM, Linköping University, 581 83 Linköping, Sweden.
Steel
Daniella
D
Asp
Julia
J
Dahlenborg
Kerstin
K
Jonsson
Marianne
M
Jeppsson
Anders
A
Lindahl
Anders
A
Kågedal
Bertil
B
Sartipy
Peter
P
Mandenius
Carl-Fredrik
CF
D015415:Biomarkers / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002452:Cell Count; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000378:metabolism*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006331:Heart Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D020349:Surface Plasmon Resonance; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D020107:Troponin T / Q000378:metabolism
D015415:Biomarkers; C493408:FABP3 protein, human; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D020107:Troponin T; D004317:Doxorubicin