The UV-derivative spectrophotometry for the determination of doripenem in the presence of its degradation products.
A spectrophotometric method was developed for the quantitative determination of doripenem in pharmaceutical dosage form (DORIBAX) in the presence of its degradation products. The first-derivative with or without the substration technique, depending on formed products degradation was applied (lambda=324 nm). The method was linear in the range concentration (0.42-11.30)x10(-2)mg L(-1) (r=0.9981), the limits of detection and quantification were 7.60 and 45.0 microg L(-1), respectively. Recovery of doripenem ranged from 99.85 to 102.97% in pharmaceutical dosage form. This method had a good precision (RDS from 0.35 to 2.93%). The observed rate constants for doripenem degradation were comparable to those obtained in recommended HPLC method.
Cielecka-Piontek
J
J
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, Poland. jpiontek@ump.edu.pl
Jelińska
A
A
D002138:Calibration; D015780:Carbapenems / Q000737:chemistry* / Q000378:metabolism*; D000077726:Doripenem; D057230:Limit of Detection; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet / Q000379:methods* / Q000592:standards; D014867:Water / Q000737:chemistry
D015780:Carbapenems; D004364:Pharmaceutical Preparations; D012996:Solutions; D014867:Water; D000077726:Doripenem