[Role of platelet NO synthase in insulin-mediated antiaggregability in patients with 2 diabetes mellitus].
It was established that insulin caused a reduction in the parameters of collagen-induced platelet aggregability in healthy donors; this effect of insulin on platelets was not found in patients with type 2 diabetes mellitus. In the healthy donors, joint incubation of platelets with insulin and L-arginine (a NO synthase substrate) resulted in an even more pronounced decrease in collagen-induced platelet aggregability while the group of patient showed a reduction in the degree and rate of the aggregability only along the light scattering curve. In the healthy individuals, addition of the NO synthase blocker LNMMA to the incubation medium containing platelets and insulin, as well as that of insulin and L-arginine eliminated insulin antiaggregability. The effect of insulin on platelet aggregation seems to be displayed with the involvement of platelet NO synthase and NO-dependent mechanisms. The change in the antiaggregability of insulin in patients with type 2 diabetes mellitus may be due to both lowered platelet NO synthase activity and insulin receptor dysfunction.
Gruzdeva
O V
OV
Suslova
T E
TE
Kremeno
S V
SV
Levashkina
E A
EA
D001792:Blood Platelets / Q000201:enzymology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
D007328:Insulin; D010975:Platelet Aggregation Inhibitors; D019001:Nitric Oxide Synthase