Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L.
Present report is aimed to study the batch kinetics of Lantana camara. Dynamic changes of parameters, such as pH, conductivity, wet and dry cell concentrations, consumption of major nutrients, carbon source and agitation speeds were investigated to understand the culture characteristics of suspended cells grown on MS + BAP + 2,4-D + NAA in shake flasks. Results indicated that the consumption of phosphate resulted in the onset of stationary phase in cultures. Maltose as carbon source resulted in production of maximum triterpenoid content (31.08 mg/L) while the least was found on glucose (10.69 mg/L). Notably, both did not support accumulation of betulinic acid. Sucrose, although stood second in terms of quantity (21.6 mg/L), supported the production of all the three triterpenoids-oleanolic, ursolic and betulinic acids. Maximum viable cultures were obtained at a rotation speed of 120 rpm. The present finding will form a background for further scale-up related studies.
Srivastava
Priyanka
P
Department of Biotechnology, Indian Institute of Technology-Guwahati, Guwahati 781039, Assam, India.
Sisodia
Vikash
V
Chaturvedi
Rakhi
R
D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D018929:Cell Culture Techniques; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D032410:Lantana / Q000166:cytology / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D014315:Triterpenes / Q000032:analysis / Q000378:metabolism*
D003470:Culture Media; D014315:Triterpenes; D013395:Sucrose; D008320:Maltose; D002244:Carbon; D005947:Glucose