Preparation and in-vitro/in-vivo evaluation of surface-modified poly (lactide-co-glycolide) fluorescent nanoparticles.
OBJECTIVE
The aim was to develop biodegradable nanoparticles suitable for cellular delivery of chemotherapeutic drugs.


METHODS
Poly (lactide-co-glycolide) (PLGA) nanoparticles were prepared using a modified solvent evaporation method. Chitosan and calcium chloride were tested as surface modifiers. Coumarin-6 was incorporated into some formulations as a fluorescent marker.


RESULTS
The median size of the particles was between 400 nm and 7 microm, and scanning electron microscope pictures showed that the particles were smooth and spherical. The zeta potentials of the particles with and without surface modifier ranged between -25.7 mV and -7.0 mV, respectively. Fluorescence microscopy and flow cytometry (FACS) analysis showed that smaller surface-modified particles were efficiently internalised by neoplastic 4T1 cells. Image analysis of frozen tissue sections from Balb/c mice given nanoparticles via the tail vein showed that the particles were distributed preferentially into the lungs, followed by the liver, spleen, kidney and heart.


CONCLUSIONS
Chitosan-modified PLGA nanoparticles showed significant uptake by neoplastic 4T1 cells, and were distributed to several major organs frequently seen as sites of cancer metastasis in mice.
Pamujula
Sarala
S
Center for Nanomedicine and Drug Delivery, Xavier University College of Pharmacy, New Orleans, LA 70125-1098, USA.
Hazari
Sidhartha
S
Bolden
Gevoni
G
Graves
Richard A
RA
Chinta
Dakshina M Devanga
DM
Dash
Srikanta
S
Kishore
Vimal
V
Mandal
Tarun K
TK
D000818:Animals; D001692:Biological Transport; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D048271:Chitosan; D003374:Coumarins; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics*; D005260:Female; D005434:Flow Cytometry; D005453:Fluorescence; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods*; D013844:Thiazoles; D014018:Tissue Distribution
D003374:Coumarins; D004337:Drug Carriers; D013844:Thiazoles; C517282:coumarin 6; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan