Oxidative stress in hypercholesterolemia and its association with Ala16Val superoxide dismutase gene polymorphism.
OBJECTIVE
To investigate the role of the oxidative stress and the antioxidant system as well as the influence of the manganese superoxide dismutase (Ala16Val) polymorphism on hypercholesterolemia.


METHODS
Levels of glucose, lipid, high-sensitivity C reactive protein (hs-CRP), thiobarbituric acid reactive substances (TBARS), carbonyl protein, thiols, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and vitamin C, vitamin E, as well as the presence of the manganese superoxide dismutase (Ala16Val) polymorphism were determined in 40 subjects with hypercholesterolemia and 40 controls.


RESULTS
Lipid profile, hs-CRP, glucose, TBARS, carbonyl protein, CAT, and vitamin E were significantly higher in subjects with hypercholesterolemia. In contrast, GSH and SOD were lower. TBARS, carbonyl protein, thiols, CAT, and vitamin E were significantly higher in hypercholesterolemic subjects with VV genotype for MnSOD, while GSH, SOD, and vitamin C were lower in these subjects.


CONCLUSIONS
We suggest an association between the genotypes of MnSOD, hypercholesterolemia, and oxidative stress biomarkers.
Duarte
Marta M M F
MM
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. duartmm@hotmail.com
Moresco
Rafael N
RN
Duarte
Thiago
T
Santi
Adriana
A
Bagatini
Margarete D
MD
Da Cruz
Ivana B M
IB
Schetinger
Maria R C
MR
Loro
Vânia L
VL
D000975:Antioxidants; D015415:Biomarkers; D016022:Case-Control Studies; D005838:Genotype; D006801:Humans; D006937:Hypercholesterolemia / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D020125:Mutation, Missense; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics*; D000099156:Superoxide Dismutase 2
D000975:Antioxidants; D015415:Biomarkers; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2