Determination of human serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) by HPLC-ECD combined with solid phase extraction (SPE).
Human serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured by HPLC-ECD method combined with solid phase extraction (SPE) developed by our group: (our proprietary kit, named 8-OHdG Pre-treatment Kit (TANITA Corporation)). The major interfering substances and proteins in serum were removed by 8-OHdG Pre-treatment Kit. This measurement method was highly reproducible (CV=2.2-7.1%) and demonstrated the lower detection limit for control serum sample of less than 10 pg/ml without the sample evaporation. The other hand 8-OHdG concentration in serum for healthy people was in the range of 0-70 pg/ml (25.5+/-13.8 pg/ml, n=37). Secondary a relationship between the HPLC-ECD and ELISA methods was investigated. ELISA method could not detect 8-OHdG concentration in serum for healthy people, because the detection limit of 130 pg/ml was higher than the normal range for healthy people. These results show our SPE method has high sensitivity and quantitative accuracy for 8-OHdG analysis.
Koide
Satoshi
S
Biotechnology Industrialization Center, National Institute of Advanced Industrial Science and Technology, c/o Katayanagi Advanced Research Laboratories, Tokyo University of Technology, 1404-1 Katakura-Machi, Hachioji, Tokyo 192-0982, Japan. satoshi.koide@tanita.co.jp
Kinoshita
Yuri
Y
Ito
Narushi
N
Kimura
Jun
J
Yokoyama
Kenji
K
Karube
Isao
I
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D055815:Young Adult
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine