title: Genotyping of Porcine teschovirus from nervous tissue of pigs with and without polioencephalomyelitis in Indiana.
abstract: Porcine teschovirus (PTV) was isolated in cell culture and/or demonstrated by polymerase chain reaction in samples of brain and/or spinal cord in pigs in Indiana during the 2002-2007 period. Testing was initiated on pigs originating from populations exhibiting nervous clinical disease and/or pigs with microscopic lesions in central nervous tissues, indicating viral encephalitis and/or myelitis. Virus was demonstrated in pigs with and without lesions as well as with and without nervous clinical disease. Nucleotide sequence analysis of the 5'-nontranslated region of the viral genome revealed that these isolates had low-level genetic heterogeneity but were homologous to porcine PTV serotype 1 (PTV-1). These findings indicate that low-to-moderate virulence strains of PTV with some homology to PTV-1 are endemic in many swineherds of Indiana and are associated with subclinical and clinical nervous disease in weaned pigs.
authors.affiliation: Animal Disease Diagnostic Laboratory and Department of Comparative Pathobiology, Purdue University, 406 South University Street, West Lafayette, IN 47907, USA.
authors.forename: Dinesh S
authors.initials: DS
authors.lastname: Bangari
authors.forename: Roman M
authors.initials: RM
authors.lastname: Pogranichniy
authors.forename: Tom
authors.initials: T
authors.lastname: Gillespie
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology*; D004679:Encephalomyelitis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005838:Genotype; D007196:Indiana / Q000453:epidemiology; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D013116:Spinal Cord / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D052700:Teschovirus / Q000235:genetics*