title: The possible involvement of copper-containing nitrite reductase (NirK) and flavohemoglobin in denitrification by the fungus Cylindrocarpon tonkinense.
abstract: The occurrence of denitrification and nitrate respiration among eukaryotes has been established during the last few decades. However, denitrification-related eukaryotic genes have been isolated from only a few fungi, and eukaryotic denitrification (or nitrate respiration) is still inadequately understood. In this study, we identified genes that were up-regulated under denitrifying conditions in the fungus Cylindrocarpon tonkinense using the suppression subtraction hybridization technique, and the expression patterns of these genes were characterized by Northern analysis. We identified copper-containing nitrite reductase, cytochrome P450 nitric oxide reductase, flavohemoglobin (Fhb), and formate/nitrite transporter homolog genes as possibly involved in fungal denitrification. Our results concerning the involvement of Fhb and formate/nitrite transporter perhaps provide new insight into the fungal denitrification system.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo.
authors.forename: Sang-Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Shengmin
authors.initials: S
authors.lastname: Zhou
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
mesh_terms: D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D009572:Nitrite Reductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins; D003300:Copper; D009572:Nitrite Reductases