title: Deregulation of Aurora kinase gene expression in human testicular germ cell tumours.
abstract: The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.17 +/- 0.30 fold (P < 0.05) and reduced in 9 to 0.38 +/- 0.10 (P < 0.01). Aurora-B mRNA was increased in 11 tumour tissues by 4.33 +/- 0.82 fold (P < 0.01) and reduced in 3 to 0.41 +/- 0.11 fold. Aurora-C mRNA was reduced to 0.20 +/- 0.32 fold (P < 0.01) in 13 seminomas and up-regulated in one case. Western blot experiments, performed on protein extracts of nine seminomas and six normal testes, showed an up-regulation of Aurora-B protein by 10.14 +/- 3.51 fold (P < 0.05), while Aurora-A protein was found increased in four seminomas by 2.16 +/- 0.43 (P < 0.05), unchanged in three and reduced in two tumour tissues. Aurora-C protein was increased by 9.2 +/- 2.90 fold (P < 0.05), suggesting that post-transcriptional mechanisms modulate its expression. In conclusion, we demonstrated that expression of Aurora kinases is deregulated in seminomas, suggesting that they may play a role in the progression of testicular cancers.
authors.affiliation: Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Baldini
authors.forename: Y
authors.initials: Y
authors.lastname: Arlot-Bonnemains
authors.forename: M
authors.initials: M
authors.lastname: Mottolese
authors.forename: S
authors.initials: S
authors.lastname: Sentinelli
authors.forename: B
authors.initials: B
authors.lastname: Antoniani
authors.forename: S
authors.initials: S
authors.lastname: Sorrenti
authors.forename: M
authors.initials: M
authors.lastname: Salducci
authors.forename: E
authors.initials: E
authors.lastname: Comini
authors.forename: S
authors.initials: S
authors.lastname: Ulisse
authors.forename: M
authors.initials: M
authors.lastname: D'Armiento
mesh_terms: D000328:Adult; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger; D018239:Seminoma / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D013737:Testis / Q000201:enzymology*; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; C576921:AURKB protein, human; C576927:AURKC protein, human; D064107:Aurora Kinase B; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases