title: Evaluation of antiproliferative, anti-type 2 diabetes, and antihypertension potentials of ellagitannins from strawberries (Fragaria × ananassa Duch.) using in vitro models.
abstract: Strawberries represent the main source of ellagic acid derivatives in the Brazilian diet, corresponding to more than 50% of all phenolic compounds found in the fruit. There is a particular interest in the determination of the ellagic acid content in fruits because of possible chemopreventive benefits. In the present study, the potential health benefits of purified ellagitannins from strawberries were evaluated in relation to the antiproliferative activity and in vitro inhibition of α-amylase, α-glucosidase, and angiotensin I-converting enzyme (ACE) relevant for potential management of hyperglycemia and hypertension. Therefore, a comparison among ellagic acid, purified ellagitannins, and a strawberry extract was done to evaluate the possible synergistic effects of phenolics. In relation to the antiproliferative activity, it was observed that ellagic acid had the highest percentage inhibition of cell proliferation. The strawberry extract had lower efficacy in inhibiting the cell proliferation, indicating that in the case of this fruit there is no synergism. Purified ellagitannins had high α-amylase and ACE inhibitory activities. However, these compounds had low α-glucosidase inhibitory activity. These results suggested that the ellagitannins and ellagic acid have good potential for the management of hyperglycemia and hypertension linked to type 2 diabetes. However, further studies with animal and human models are needed to advance the in vitro assay-based biochemical rationale from this study.
authors.affiliation: Department of Food and Experimental Nutrition, University of São Paulo, São Paulo, Brazil.
authors.forename: Marcia da Silva
authors.initials: Mda S
authors.lastname: Pinto
authors.forename: Joao Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.forename: Franco Maria
authors.initials: FM
authors.lastname: Lajolo
authors.forename: Maria Inés
authors.initials: MI
authors.lastname: Genovese
authors.forename: Kalidas
authors.initials: K
authors.lastname: Shetty
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D031985:Fragaria / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D008517:Phytotherapy; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D047348:Hydrolyzable Tannins; D000516:alpha-Amylases