The regulatory mechanism of β-eudesmol is through the suppression of caspase-1 activation in mast cell-mediated inflammatory response.
β-Eudesmol is sesquiterpenoid alcohol which contains the rhizome of Atractylodes lancea. Although it has multiple pharmacological effects, the anti-inflammatory effect of β-eudesmol and its molecular mechanisms are poorly elucidated. In this study, we investigated the regulatory mechanism of β-eudesmol on mast cell-mediated inflammatory response. The results indicated that β-eudesmol inhibited the production and expression of interleukin (IL)-6 on phorbol 12-myristate 13-acetate and calcium ionophore A23187-stimulated human mast cell (HMC). In activated HMC-1 cells, β-eudesmol suppressed activation of p38 mitogen-activated protein kinase (MAPKs) and nuclear factor-κB. In addition, β-eudesmol suppressed the activation of caspase-1 and expression of receptor-interacting protein-2. These results provide new insights into the pharmacological actions of β-eudesmol as a potential molecule for use in therapy in mast cell-mediated inflammatory diseases.
Seo
Min-Jun
MJ
Department of Medicinal Herb Development, Gyeongju University, Gyeongbuk, Republic of Korea.
Kim
Su-Jin
SJ
Kang
Tae-Hee
TH
Rim
Hong-Kun
HK
Jeong
Hyun-Ja
HJ
Um
Jae-Young
JY
Hong
Seung-Heon
SH
Kim
Hyung-Min
HM
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D061945:Caspase Inhibitors; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology; D010766:Phosphorylation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045787:Sesquiterpenes, Eudesmane / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D061945:Caspase Inhibitors; D015850:Interleukin-6; D016328:NF-kappa B; D045787:Sesquiterpenes, Eudesmane; D000001:Calcimycin; C051082:beta-eudesmol; D048051:p38 Mitogen-Activated Protein Kinases; D013755:Tetradecanoylphorbol Acetate