title: Highly efficient chemoenzymatic synthesis of beta1-4-linked galactosides with promiscuous bacterial beta1-4-galactosyltransferases.
abstract: Two bacterial beta1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous and complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, and their derivatives including those containing negatively charged 6-O-sulfated GlcNAc and C2-substituted GlcNAc or Glc, from monosaccharide derivatives and inexpensive Glc-1-P.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Musleh M
authors.initials: MM
authors.lastname: Muthana
authors.forename: Denton
authors.initials: D
authors.lastname: Wong
authors.forename: Ronald
authors.initials: R
authors.lastname: Huang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005697:Galactosides / Q000138:chemical synthesis* / Q000737:chemistry; D016480:Helicobacter pylori / Q000201:enzymology*; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry* / Q000235:genetics; D009345:Neisseria meningitidis / Q000201:enzymology*; D016979:Pasteurella multocida / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D005697:Galactosides; D011993:Recombinant Fusion Proteins; D009237:N-Acetyllactosamine Synthase