title: Development of molecular tools for characterization and genetic diversity analysis in Tunisian fig (Ficus carica) cultivars.
abstract: Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on the combined data show considerable genetic diversity, and the tested markers discriminated all fig genotypes studied. The identification key established on the basis of SSR permitted the unambiguous discrimination of cultivars and confirmed the reliability of SSR for fingerprinting fig genotypes. The study findings are discussed in relation to the establishment of a national reference collection that will aid in the conservation of Tunisian fig resources.
authors.affiliation: Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092, El Manar, Tunis, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Chatti
authors.forename: Ghada
authors.initials: G
authors.lastname: Baraket
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ben Abdelkrim
authors.forename: Olfa
authors.initials: O
authors.lastname: Saddoud
authors.forename: Messaoud
authors.initials: M
authors.lastname: Mars
authors.forename: Mokhtar
authors.initials: M
authors.lastname: Trifi
authors.forename: Amel
authors.initials: A
authors.lastname: Salhi Hannachi
mesh_terms: D030681:Ficus / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005787:Gene Frequency; D005788:Gene Pool; D005819:Genetic Markers / Q000235:genetics; D005821:Genetic Techniques; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers