[Effect of the compound of poly lactic-co-glycolic acid and bone marrow stromal cells modified by osteoprotegerin gene on the periodontal regeneration in Beagle dog periodontal defects].
OBJECTIVE
To evaluate the effect of the osteoprotegerin (OPG) gene-modified autologous bone marrow stromal cells (BMSCs) on regeneration of periodontal defects, and to provide new experimental evidence to explore the gene therapy for periodontal disease.


METHODS
pSecTag2/B-opg was transduced into BMSCs by lipofectamine 2000. The expression of OPG protein in the BMSCs was detected by immunocytochemistry and Western blot. Inverted phase contrast microscope and scanning electron microscopy (SEM) were used to observe the morphology and proliferation of the BMSCs(OPG) on on the surface of the poly lactic-co-glycolic (PLGA). Horizontal alveolar bone defect (4 mmx4 mmx 3 mm) were surgically created in the buccal aspect of the mandibular premolar, and were randomly assigned to receive BMSCs(OPG)-PLGA (cells/material/OPG), BMSCs-PLGA (cells/material), PLGA (material), or root planning only (blank control). The animals were euthanized at 6 weeks post surgery for histological analysis. The height of new alveolar bone and cementum and the formation of new connective tissue were analyzed and compared. All data were statistically analyzed using the q test.


RESULTS
The BMSCs transfected by human OPG gene can highly express OPG protein. SEM observations demonstrated that BMSCs(OPG) were able to proliferate and massively colonize on the scaffolds structure. After 6 weeks, the height of new alveolar bone and cementum and the formation of new connective tissue were significantly greater in the experimental group than in the control groups (P < 0.05).


CONCLUSIONS
BMSCs(OPG)-PLGA can significantly promote the regeneration of dog's periodontal bone defects. Gene therapy utilizing OPG may offer the potential for periodontal tissue engineering applications.
Zhou
Wei
W
Dept. of Oral Medicine, The Affiliated Hospital of Stomatology, Anhui Medical University, Hefei 230032, China.
Zhao
Chun-Hui
CH
Mei
Ling-Xuan
LX
D000818:Animals; D001861:Bone Regeneration; D003739:Dental Cementum; D004285:Dogs; D006016:Glycolates; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells; D053244:Osteoprotegerin; D011091:Polyesters; D011108:Polymers; D012038:Regeneration; D023822:Tissue Engineering
D006016:Glycolates; D053244:Osteoprotegerin; D011091:Polyesters; D011108:Polymers; C031149:glycolic acid; D019344:Lactic Acid; C033616:poly(lactide)