Evaluation of human osteosarcoma cell line genotoxicity effects of mineral trixoide aggregate and calcium silicate cements.
BACKGROUND
Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63).


METHODS
A mitochondrial colorimetric assay was used to evaluate the MG63 survival rate. A DNA precipitation assay was used to detect the MG63 DNA damage after contact with MTA or CS cement extracts.


RESULTS
The results showed that MTA and CS are cytocompatible with MG63 cells. There was no significant difference in the survival rate with MTA and CS materials (P > .05). Neither MTA nor CS cements causing MG63 cell DNA damage showed significant genotoxicity (P > .05).


CONCLUSIONS
Both MTA and CS cements are compatible with MG63 cells, and they are not cancer-causing agents.
Ding
Shinn-Jyh
SJ
Institute of Oral Biology and Biomaterials, Chung Shan Medical University, Taichung, Taiwan.
Kao
Chia-Tze
CT
Chen
Chih-Lin
CL
Shie
Ming-You
MY
Huang
Tsui-Hsien
TH
D017607:Aluminum Compounds / Q000633:toxicity*; D000537:Aluminum Oxide / Q000032:analysis; D001672:Biocompatible Materials / Q000633:toxicity; D017610:Calcium Compounds / Q000032:analysis / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004247:DNA / Q000032:analysis; D004249:DNA Damage; D004338:Drug Combinations; D005290:Ferric Compounds / Q000032:analysis; D006801:Humans; D008277:Magnesium Oxide / Q000032:analysis; D009153:Mutagens / Q000633:toxicity*; D012516:Osteosarcoma / Q000473:pathology*; D010087:Oxides / Q000032:analysis / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000032:analysis / Q000633:toxicity*; D012822:Silicon Dioxide / Q000032:analysis; D015034:Zinc Oxide / Q000032:analysis
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D005290:Ferric Compounds; D009153:Mutagens; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C000499:ferric oxide; D008277:Magnesium Oxide; D012822:Silicon Dioxide; D004247:DNA; C016538:lime; D000537:Aluminum Oxide; C031293:calcium silicate; D015034:Zinc Oxide