Desorption electrospray ionization tissue imaging using heated nebulizing gas and high-resolution accurate mass spectra.
A new method for tissue imaging using desorption electrospray ionization (DESI) mass spectrometry is described. The technique utilizes a DESI source with a heated nebulizing gas and high-resolution accurate mass data acquired with an LTQ-Orbitrap mass spectrometer. The two-dimensional (2D) automated DESI ion source creates images using the ions that are collected under high-resolution conditions. The use of high-resolution mass detection significantly improves the image quality due to exclusion of interfering ions. The use of a heated nebulizing gas increases the signal intensity observed at lower gas pressure. The technique developed is highly compatible with soft tissue imaging due to the minimal surface destruction.
Waldon
Daniel J
DJ
Pharmacokinetics and Drug Metabolism, Amgen Inc., Cambridge, Massachusetts 02142, USA.
Zhao
Zhiyang
Z
Teffera
Yohannes
Y
D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D005740:Gases / Q000737:chemistry; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D005740:Gases