Stability of 0.5% cidofovir stored under various conditions for up to 6 months.
OBJECTIVE
To evaluate the effect of time, temperature and storage vial material on the antiviral activity of 0.5% cidofovir solution.


METHODS
Commercial 7.5% cidofovir solution for injection was diluted with normal saline to a 0.5% concentration. Aliquots were stored in plastic and glass vials at 4, -20, and -80 degrees C for 30, 60, 120, and 180 days. Antiviral activity against feline herpesvirus was evaluated in a virus titration assay at time zero (baseline) and at each subsequent time point.


RESULTS
Cidofovir caused a fourfold log reduction in virus titer at baseline and at each time point and for each storage condition (P < 0.001).


CONCLUSIONS
0.5% cidofovir demonstrated stable antiviral activity when stored for up to 6 months in glass or plastic, at 4, -20, and -80 degrees C.
Stiles
Jean
J
Department of Veterinary Clinical Sciences, Animal Disease Diagnostic Laboratory, Purdue University School of Veterinary Medicine, West Lafayette, IN, USA. stilesj@purdue.edu
Gwin
Wilson
W
Pogranichniy
Roman
R
D000998:Antiviral Agents; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives*; D004355:Drug Stability; D004356:Drug Storage / Q000379:methods; D006564:Herpesviridae / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D063065:Organophosphonates; D013696:Temperature; D013997:Time Factors
D000998:Antiviral Agents; D063065:Organophosphonates; D003596:Cytosine; D000077404:Cidofovir