Antioxidant activity and cytotoxicity of the traditional Indonesian medicine Tahongai (Kleinhovia hospita L.) extract.
We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50 microg/mL, 87.5 microg/mL, and 125 microg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.
Arung
Enos Tangke
ET
Wood Chemistry Laboratory, Department of Forest Product Technology, Forestry Faculty, Mulawarman University, Samarinda, East Kalimantan, Indonesia. tangkearung@yahoo.com
Kusuma
Irawan Wijaya
IW
Purwatiningsih
Sri
S
Roh
Seong-Soo
SS
Yang
Chae Ha
CH
Jeon
Soohyeon
S
Kim
Yong-Ung
YU
Sukaton
Edi
E
Susilo
Joko
J
Astuti
Yuli
Y
Wicaksono
Britanto Dani
BD
Sandra
Ferry
F
Shimizu
Kuniyoshi
K
Kondo
Ryuichiro
R
D000975:Antioxidants / Q000032:analysis* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D007214:Indonesia; D019660:Malvaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts