title: Mesenchymal stromal cells use PGE2 to modulate activation and proliferation of lymphocyte subsets: Combined comparison of adipose tissue, Wharton's Jelly and bone marrow sources.
abstract: Due to their immunomodulatory properties, adipose tissue (AT) and Wharton's Jelly (WJ) constitute valuable alternatives to BM as sources of MSCs for managing graft-versus-host disease. To ensure the efficiency of AT- and WJ-MSCs implies the characterization of their immunomodulatory functions in comparison to those of BM. In this study, we investigated the capacity of AT- and WJ-MSCs to modulate lymphocyte reactions in response to different stimuli as well as the specificity of this immunomodulation. AT- and WJ-MSC displayed potent immunosuppressive effects on lymphocyte responses in a dose-dependent manner. These effects included the prevention of lymphocyte activation as well as the suppression of T-cell proliferation regardless of the stimuli used to activate lymphocytes. These effects were mediated through the expression of COX1/COX2 enzymes and by the production of PGE2. CD4(+) and CD8(+) T-lymphocytes were equally targeted by MSCs demonstrating that the immunomodulation was not restricted to a specific T-cell subpopulation.
authors.affiliation: Laboratory of Experimental Hematology, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, Belgium. mnajar@ulb.ac.be
authors.forename: Mehdi
authors.initials: M
authors.lastname: Najar
authors.forename: Gordana
authors.initials: G
authors.lastname: Raicevic
authors.forename: Hicham Id
authors.initials: HI
authors.lastname: Boufker
authors.forename: Hussein
authors.initials: H
authors.lastname: Fayyad Kazan
authors.forename: Cécile
authors.initials: C
authors.lastname: De Bruyn
authors.forename: Nathalie
authors.initials: N
authors.lastname: Meuleman
authors.forename: Dominique
authors.initials: D
authors.lastname: Bron
authors.forename: Michel
authors.initials: M
authors.lastname: Toungouz
authors.forename: Laurence
authors.initials: L
authors.lastname: Lagneaux
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D001853:Bone Marrow / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D056747:Immunomodulation; D007213:Indomethacin / Q000494:pharmacology; D007231:Infant, Newborn; D008213:Lymphocyte Activation / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014470:Umbilical Cord / Q000473:pathology*
chemical_list: D015703:Antigens, CD; D015232:Dinoprostone; D007213:Indomethacin