title: Association of glutathione S-transferase M1 and T1 gene polymorphisms and oxidative stress markers in preterm labor.
abstract: OBJECTIVE
  Oxidative stress and related gene polymorphism may be associated with the etiology of preterm labor (PTL). The present study was designed to investigate association of GSTM1 and GSTT1 gene polymorphisms with PTL and their relationship with oxidative stress markers.
  
  
  METHODS
  Sixty cases of PTL and sixty three subjects of full term labor (FTL) were included in the study. Multiplex PCR was performed for GSTM1 and GSTT1 genes polymorphism and oxidative stress markers were analyzed.
  
  
  RESULTS
  MDA and 8-OHdG levels were increased, while GSH was decreased in PTL than FTL subjects. Frequency of GSTM1-/GSTT1-(null) was significantly higher in PTL in comparison to FTL (p=0.028, OR=3.4). Subjects with GSTM1-/GSTT1+, GSTM1+/GSTT1-, GSTM1-/GSTT1- have significant differences of oxidative stress markers as compared to GSTM1+/GSTT1+ genotype.
  
  
  CONCLUSIONS
  GSTM1-/GSTT1- (null) genotype may be one of the associated genetic factor for the increased risk of PTL.
authors.affiliation: Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, Dilshad Garden, Delhi 110 095, India.
authors.forename: M D
authors.initials: MD
authors.lastname: Mustafa
authors.forename: Rahul
authors.initials: R
authors.lastname: Pathak
authors.forename: Tanzeel
authors.initials: T
authors.lastname: Ahmed
authors.forename: Rafat S
authors.initials: RS
authors.lastname: Ahmed
authors.forename: A K
authors.initials: AK
authors.lastname: Tripathi
authors.forename: Kiran
authors.initials: K
authors.lastname: Guleria
authors.forename: B D
authors.initials: BD
authors.lastname: Banerjee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D004252:DNA Mutational Analysis; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007752:Obstetric Labor, Premature / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1