[Effect of PTU on the expression of thyroid TPO, Tg mRNA in rats].
OBJECTIVE
To explore the mechanism and the compensation proceeding of thyroid hyperplasia caused by PTU.


METHODS
PTU was administered to rats by gavage at a dose of 5.0 mg/kg B. W for 3, 6, 9, 12, 15, 18, 21 days respectively. All animals were sacrificed after the last dosage, and the expression of TPO and Tg in thyroid was detected by RT-PCR. The serum thyroid hormones were measured by chemoluminescence.


RESULTS
Compared with the control group, the TPO mRNA levels of PTU groups were increased, while the Tg mRNA levels were decreased significantly. Serum TT3, TT4 in rats treated with PTU demonstrated a descending trend, while serum TSH showed an ascending trend, and the significant differences were observed after 6 days treating with PTU.


CONCLUSIONS
The interference of PTU on thyroid may relate to inhibiton of Tg gene transcription. The enhancement of TPO gene transcription can't compensate the thyroid function sufficiently.
Shang
Wei-Wei
WW
Department of Patholgy, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Luo
Jian
J
Cheng
Wei-Bo
WB
Huang
Qin
Q
Wang
Wen-Dong
WD
D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D006965:Hyperplasia / Q000139:chemically induced; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D011441:Propylthiouracil / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013954:Thyroglobulin / Q000235:genetics / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology
D052244:Endocrine Disruptors; D012333:RNA, Messenger; D011441:Propylthiouracil; D013954:Thyroglobulin; D007453:Iodide Peroxidase