Nicotinic acetylcholine receptor activation reduces skeletal muscle inflammation of mdx mice.
Mdx mice develop an inflammatory myopathy characterized at different ages by myonecrosis with scattered inflammatory infiltrates followed by muscular regeneration and later persistent fibrosis. This work aimed to verify the putative anti-inflammatory role of nicotinic acetylcholine receptor (nAChR) in the mdx muscular lesion. Mitigation of myonecrosis and decreased TNFα production were accompanied by increased numbers of F4/80 macrophages expressing nAChRα7. In vivo treatment with nicotine attenuated muscular inflammation characterized by reduced metalloprotease MMP-9 activity, TNFα and NFkB content and increased muscular regeneration. Our data indicate that nAChR activation influences local inflammatory responses in the muscular lesion of mdx mice.
Leite
Paulo Emílio Corrêa
PE
Laboratório de Patologia Celular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Lagrota-Candido
Jussara
J
Moraes
Louise
L
D'Elia
Livia
L
Pinheiro
Douglas Florindo
DF
da Silva
Rafael Ferreira
RF
Yamasaki
Edna N
EN
Quirico-Santos
Thereza
T
D000818:Animals; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D020388:Muscular Dystrophy, Duchenne / Q000276:immunology / Q000473:pathology / Q000628:therapy; D009220:Myositis / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D009336:Necrosis; D021122:Protein Subunits / Q000378:metabolism / Q000502:physiology; D011978:Receptors, Nicotinic / Q000378:metabolism* / Q000502:physiology; D064569:alpha7 Nicotinic Acetylcholine Receptor
C579166:Chrna7 protein, mouse; D018836:Inflammation Mediators; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor