title: The combination of fructooligosaccharides and resistant starch shows prebiotic additive effects in rats.
abstract: Different types of dietary fiber can be distinguished considering their rate of fermentability, thus determining the location of the large intestine where they exert their beneficial effect. Their combination could be interesting to obtain health-promoting effects throughout the entire colon. The aim of the present study was to evaluate the synergistic effect of two dietary fibers with different fermentation patterns, fructooligosaccharides (FOS) (Beneo(®)-95) and resistant starch (Fibersol(®)-2), after their administration to healthy rats or in trinitrobenzenesulphonic acid-(TNBS) colitic rats, with an altered colonic immune response. In healthy rats, the administration of the combination of FOS and resistant starch induced changes in the intestinal microbiota, by increasing lactobacilli and bifidobacteria in caecum and colonic contents. Furthermore, its administration up-regulated the expression of the trefoil factor-3 and MUC-2 in comparison with untreated rats, thus improving the intestinal barrier function. The beneficial effects observed with this combination were confirmed in the TBNS model of rat colitis, since it was able to exert intestinal anti-inflammatory effect, associated with an increase of protective bacteria and up-regulation of epithelial defense mechanisms. In conclusion, the combination of two different dietary fibers may result in a synergistic prebiotic effect, and may confer greater health benefits to the host.
authors.affiliation: Centro de Investigaciones Biomédicas en Red - Enfermedades Hepáticas y Digestivas (CIBER-EHD), Department of Pharmacology, Center for Biomedical Research, University of Granada, Granada, Spain.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Rodríguez-Cabezas
authors.forename: Desirée
authors.initials: D
authors.lastname: Camuesco
authors.forename: Belén
authors.initials: B
authors.lastname: Arribas
authors.forename: Natividad
authors.initials: N
authors.lastname: Garrido-Mesa
authors.forename: Mònica
authors.initials: M
authors.lastname: Comalada
authors.forename: Elvira
authors.initials: E
authors.lastname: Bailón
authors.forename: Margarita
authors.initials: M
authors.lastname: Cueto-Sola
authors.forename: Pilar
authors.initials: P
authors.lastname: Utrilla
authors.forename: Eduardo
authors.initials: E
authors.lastname: Guerra-Hernández
authors.forename: Carlos
authors.initials: C
authors.lastname: Pérez-Roca
authors.forename: Julio
authors.initials: J
authors.lastname: Gálvez
authors.forename: Antonio
authors.initials: A
authors.lastname: Zarzuelo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003092:Colitis / Q000378:metabolism / Q000382:microbiology*; D003106:Colon / Q000378:metabolism / Q000382:microbiology; D004043:Dietary Fiber / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005285:Fermentation; D007778:Lactobacillus / Q000302:isolation & purification; D009844:Oligosaccharides / Q000378:metabolism*; D056692:Prebiotics / Q000382:microbiology*; D051381:Rats; D017208:Rats, Wistar; D013213:Starch / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000893:Anti-Inflammatory Agents; D004043:Dietary Fiber; D009844:Oligosaccharides; D056692:Prebiotics; C116580:fructooligosaccharide; D013213:Starch