Evolutionary origin of rhopalia: insights from cellular-level analyses of Otx and POU expression patterns in the developing rhopalial nervous system.
In Cnidaria, the medusae of Scyphozoa and its sister-group Cubozoa uniquely possess rhopalia at their bell margin. These sensory centers coordinate behavior and development. We used fluorescent in situ hybridization and confocal microscopy to examine mRNA expression patterns in Aurelia sp.1 (Cnidaria, Scyphozoa) during early medusa formation, while simultaneously visualizing the developing nervous system by immunofluorescence. The genes investigated include AurOtx1, and the POU genes, AurPit1, and AurBrn3, homologs of genes known to function in cephalar neural organization and sensory cell differentiation across Bilateria. Our results show that AurOtx1 expression defines the major part of the oral neuroectodermal domain of the rhopalium, within which distinct populations of AurBrn3- and AurPit1-expressing sensory cells develop. Thus, despite the unique attributes of rhopalial evolution, we suggest that the rhopalial nervous system of scyphozoan medusae involves similar patterns of differential expression of genes that function in bilaterian cephalic structure and neuroendocrine system development. We propose that rhopalia evolved from preexisting sensory structures that developed distinct populations of sensory cells differentially expressing POU genes within Otx oral-neuroectodermal domains. This implies some commonality of developmental genetic functions involving these genes in the still poorly constrained common ancestor of bilaterians and cnidarians.
Nakanishi
Nagayasu
N
Department of Ecology and Evolutionary Biology, UCLA, 621 Young Drive South, Los Angeles, CA 90095-1606, USA. nagayasu.nakanishi@sars.uib.no
Yuan
David
D
Hartenstein
Volker
V
Jacobs
David K
DK
D000818:Animals; D005075:Biological Evolution; D003063:Cnidaria / Q000033:anatomy & histology* / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000378:metabolism; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D009420:Nervous System / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D051857:Otx Transcription Factors / Q000378:metabolism*; D050809:POU Domain Factors / Q000378:metabolism*; D012333:RNA, Messenger; D037401:Scyphozoa / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D050818:Transcription Factor Brn-3 / Q000378:metabolism; D050817:Transcription Factor Pit-1 / Q000378:metabolism
D018398:Homeodomain Proteins; D051857:Otx Transcription Factors; D050809:POU Domain Factors; D012333:RNA, Messenger; D050818:Transcription Factor Brn-3; D050817:Transcription Factor Pit-1