Aptamer-based electrochemical approach to the detection of thrombin by modification of gold nanoparticles.
This paper presents a simple electrochemical approach for the detection of thrombin, using aptamer-modified electrodes. The use of gold nanoparticles results in significant signal enhancement for subsequent detection. 1,6-Hexanedithiol was used as the medium to link Au nanoparticles to a bare gold electrode. Anti-thrombin aptamers were immobilized on the gold nanoparticles' surfaces by self-assembly. The packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH(3))(6)](3+)) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode, leading to increased sensitivity of the aptasensor and a detection limit of 1 pmol L(-1). Based on the Langmuir model, the sensor signal displayed an almost perfect linear relationship over the range of 1 pmol L(-1) to 30 nmol L(-1). Moreover, the proposed aptasensor is highly selective and stable. In summary, this biosensor is simple, highly sensitive, and selective, which is beneficial to the ever-growing interest in fabricating portable bio-analytical devices with simple electrical readout procedures.
Li
Lidong
L
School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing 100191, China. lilidong@buaa.edu.cn
Zhao
Hongtao
H
Chen
Zhengbo
Z
Mu
Xiaojiao
X
Guo
Lin
L
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis*
D052157:Aptamers, Nucleotide; D013438:Sulfhydryl Compounds; C081676:thrombin aptamer; D006046:Gold; D013917:Thrombin; C501433:1,6-hexanedithiol