Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerised acrylic resin.
OBJECTIVE
This study evaluated the efficacy of disinfectants on the internal aspect of heat-polymerised acrylic resin contaminated with microbial strains.


BACKGROUND
Dentures absorb oral fluids and become contaminated by different microorganisms.


METHODS
Two hundred and fifty rectangular specimens were made of heat-polymerised acrylic resin, and then divided into five groups corresponding to the microbial strains (Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, S. mutans and Enterococcus faecalis). After contamination, the specimens were immersed in 1 and 2% sodium hypochlorite and 2% glutaraldehyde for periods of 5, 10 and 15 min. The specimens were placed into tubes containing different broths and incubated at 35°C and then visually analysed. Turbidity in the medium indicated microbial growth. The Fisher's exact test was used in the analysis of the results.


RESULTS
The strain E. faecalis was the most resistant to the disinfectant solutions, and among them, glutaraldehyde was more effective than 2 and 1% hypochlorite for disinfection for 5 min; in the 10-min period there were no differences between the disinfectants. In 15 min of immersion, 1% hypochlorite and glutaraldehyde were more effective than 2% hypochlorite.


CONCLUSIONS
Disinfection for 10 min with 1% hypochlorite and glutaraldehyde is effective in disinfecting the internal aspect of heat-polymerised acrylic resin.
Orsi
Iara Augusta
IA
Fixed Partial Dentures, Department of Dental Materials and Prosthodontics, Dental School of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. iaraorsi@forp.usp.br
Junior
Adalton Garcia
AG
Villabona
Camilo Andrés
CA
Fernandes
Flávio Henrique Carriço Nogueira
FH
Ito
Isabel Yoko
IY
D000180:Acrylic Resins; D003470:Culture Media; D019551:Dental Disinfectants; D003778:Dentures / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D005976:Glutaral; D006801:Humans; D012973:Sodium Hypochlorite
D000180:Acrylic Resins; D003470:Culture Media; D019551:Dental Disinfectants; D012973:Sodium Hypochlorite; D005976:Glutaral