title: Cell compatibility of fibrin sealants: in vitro study with cells involved in soft tissue repair.
abstract: Fibrin sealants can be used to support tissue regeneration or as vehicles for delivery of cells in tissue engineering. Differences in the composition of fibrin sealants, however, could determine the success of such applications. The results presented in this article show clear differences between Fibrin sealant A (FS A) clots and Fibrin sealant B (FS B) clots with respect to their compatibility with primary human cells involved in soft tissue repair. FS A clots, which are characterized by a physiological coarse fibrin structure, promoted attachment, spreading, and proliferation of keratinocytes, fibroblasts, and endothelial cells. In contrast, FS B clots displaying a fine to medium clot structure failed to support spreading of all three cell types. Adhesion of keratinocytes was decreased on FS B clots compared to FS A clots after 3 h incubation, whereas number of attached fibroblasts and endothelial cells was initially comparable between the two fibrin sealants. However, all three cell types proliferated on FS A clots but no sustained proliferation was detected on FS B clots. We further demonstrate that the observed differences between FS A and B clots are partly based upon 1 M sodium chloride extractable constituents, like thrombin, and partly on nonextractable constituents or the fibrin structure. In conclusion, our in vitro results demonstrate that FS A clots serve as a provisional matrix that encourages adhesion and growth of keratinocytes, fibroblasts, and endothelial cells. Therefore, FS A seems to be well suited for applications in tissue engineering.
authors.affiliation: Baxter Innovations GmbH, Biosurgery Division Industriestrasse, Vienna, Austria.
authors.forename: Diana
authors.initials: D
authors.lastname: Macasev
authors.forename: James P
authors.initials: JP
authors.lastname: Diorio
authors.forename: Alfred
authors.initials: A
authors.lastname: Gugerell
authors.forename: Andreas
authors.initials: A
authors.lastname: Goppelt
authors.forename: Heinz
authors.initials: H
authors.lastname: Gulle
authors.forename: Michaela
authors.initials: M
authors.lastname: Bittner
mesh_terms: D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D015718:Fibrin Tissue Adhesive / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008422:Materials Testing; D023822:Tissue Engineering; D014945:Wound Healing
chemical_list: D015718:Fibrin Tissue Adhesive