Identification of up-regulated genes of Bacillus amyloliquefaciens B55 during the early stage of direct surface contact with rice R109 root.
The early stage of plant-rhizobacteria interaction, affected by plant root exudates and plant-rhizobacteria surface contact, is considered to be critical for plant growth-promoting rhizobacteria colonizing plant roots and initiating the beneficial effects on plant growth. However, little is known about the mechanisms of plant-rhizobacteria surface contact involved in early stage of plant-rhizobacteria interaction. In order to reveal the molecular mechanisms of the surface contact, a rhizobacterium Bacillus amyloliquefaciens B55 was interacted with plant roots of rice R109 and used to perform a cDNA-based suppression-subtractive hybridization. Seven differentially expressed DNA fragments were identified. Except for the two fragments showing no matches to any known sequences in the Genbank, the other five fragments were found to have high homologies with the genes encoding 2-oxoglutarate dehydrogenase E1 component OdhA, aspartate ammonia-lyase AnsB, and hypothetical protein proposed to be involved in surface adhesion, acetolactate decarboxylase AlsD, and DNA mismatch repair protein MutL, respectively. The induced RNA expression levels of two putative genes ansB and odhA and an unmatched DNA fragment BD33 were verified by RT-PCR analysis.
Liu
Jun
J
Department of Plant Pathology, College of Plant Protection, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
He
Dan
D
Ma
Xin
X
Wu
Huijun
H
Gao
Xuewen
X
D001407:Bacillus / Q000235:genetics / Q000502:physiology*; D001422:Bacterial Adhesion; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
D004269:DNA, Bacterial