Controlling thermo-reversibility of gelatin gels through a peroxidase-catalyzed reaction under mild conditions for mammalian cells.
A variety of cross-linking methods is used for obtaining gelatin gels having a tolerance to thermo-reversible gel-sol transition at physiological temperature. In this paper, we investigated the applicability of horseradish peroxidase-catalyzed cross-linking of tyrosine residues originally contained in native gelatin molecules for preparing such gelatin gels. The gelatin gels obtained through exposure to the enzymatic reaction showed a higher resistance to thermo-reversibility at 37°C than gels obtained through a thermally-induced gelation alone. In addition, the resistance property to thermo-reversible gel-sol transition was tunable by controlling enzymatic reaction conditions: higher peroxidase concentration and thermally-induced pre-gelation accomplished by cooling the gelatin solution prior to the enzymatic reaction produced gels with higher resistance to thermo-reversibility. Fibroblast cells enclosed in the gelatin gels obtained through the enzymatic reaction with thermally-induced pre-gelation showed 93% viability. These results demonstrate the feasibility of peroxidase-catalyzed reaction for obtaining gelatin gels having a tolerance to thermo-reversible gel-to-sol transition at physiological temperature toward applications in biomedical and biopharmaceutical fields.
Sakai
Shinji
S
a Division of Chemical Engineering, Department of Materials Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Moriyama
Kousuke
K
Kawakami
Koei
K
D000818:Animals; D002384:Catalysis; D002460:Cell Line; D002470:Cell Survival; D005240:Feasibility Studies; D005347:Fibroblasts / Q000502:physiology; D005780:Gelatin / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D013552:Swine; D013696:Temperature; D014867:Water / Q000737:chemistry
D020100:Hydrogels; D014867:Water; D005780:Gelatin; D006735:Horseradish Peroxidase