[Variation of endogenous hormones in formation of microtubers of Pinellia ternata in vitro].
OBJECTIVE
Through analysis of variation and function of five endogenous hormones (IAA, GA3, ABA, ZR and JA) in the formation of microtubers of Pinellia ternata in vitro to explore the physiological and biochemical mechanism of tubers' development.


METHODS
The endogenous hormones of microtubers were isolated in different stages of tubers expansion when the leaf stalks of P. ternata were cultured on MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 5% medium to induce the formation of microtubers, then them and stems were detected with Enzyme-linked Immunosorbent Assays (ELISA).


RESULTS
The results showed that the content of IAA, ABA, JA and ZR in the intumescentia period of microtubers were rapidly rising. The curve of GA3 appeared the type of anti-S.


CONCLUSIONS
IAA, ABA, JA, ZR and GA3 played an important role in controlling formation of microtubers in P. ternata.
Sheng
Wei
W
School of Life Science, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
Xue
Jianping
J
Zhang
Aimin
A
Chang
Li
L
Fan
Chengcheng
C
D000704:Analysis of Variance; D006728:Hormones / Q000378:metabolism*; D030021:Pinellia / Q000254:growth & development / Q000378:metabolism*; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D006728:Hormones