title: The activation potential of MOF is constrained for dosage compensation.
abstract: The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5' or 3' enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.
authors.affiliation: Adolf-Butenandt-Institute and Centre for Integrated Protein Science, Ludwig-Maximilians-University, 80336 Munich, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Prestel
authors.forename: Christian
authors.initials: C
authors.lastname: Feller
authors.forename: Tobias
authors.initials: T
authors.lastname: Straub
authors.forename: Heike
authors.initials: H
authors.lastname: Mitlöhner
authors.forename: Peter B
authors.initials: PB
authors.lastname: Becker
mesh_terms: D000107:Acetylation; D000818:Animals; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors; D015533:Transcriptional Activation; D014960:X Chromosome / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D051548:Histone Acetyltransferases; C111063:mof protein, Drosophila