Beta-Catenin mediates the anti-adipogenic effect of baicalin.
beta-Catenin reportedly inhibits adipogenesis through the down-regulations of peroxisome proliferator-activated receptor (PPAR)gamma and CCAAT/enhancer binding protein (C/EBP)alpha. We report that baicalin, a natural flavonoid compound, inhibits adipogenesis by modulating beta-Catenin. During 3T3-L1 cell adipogenesis, beta-Catenin was down-regulated, but baicalin treatment maintained beta-Catenin expression. Anti-adipogenic effects of baicalin were significantly attenuated by beta-Catenin siRNA transfection. beta-Catenin siRNA rescued the reduced expressions of PPARgamma, C/EBPalpha, fatty acid binding protein 4 and lipoprotein lipase by baicalin. Furthermore, baicalin modulated members of the WNT/beta-Catenin pathway by maintaining the expressions of low-density lipoprotein receptor-related protein 6, disheveled (DVL)2 and DVL3. These findings suggest that beta-Catenin mediates the anti-adipogenic effects of baicalin.
Lee
Haeyong
H
Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Republic of Korea.
Bae
Sungmin
S
Kim
Kijeong
K
Kim
Wonyong
W
Chung
Sang-In
SI
Yoon
Yoosik
Y
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*
D022763:CCAAT-Enhancer-Binding Protein-alpha; D005419:Flavonoids; D047495:PPAR gamma; D051153:Wnt Proteins; D051176:beta Catenin; C038044:baicalin