title: Synthesis of sialyllactosamine clusters using carbosilane as core scaffolds by means of chemical and enzymatic approaches.
abstract: An efficient synthesis of sialyllactosamine (SiaLacNAc) clusters using carbosilanes as core scaffolds has been accomplished by means of chemical and enzymatic approaches. N-Acetyl-D-glucosamine (GlcNAc) clusters having O-glycosidic linkage or S-glycosidic linkage were chemically synthesized from known intermediates in high yields. The GlcNAc clusters were first used as substrates for beta1,4 galactosyl transferase using UDP-galactose (UDP-Gal) as a sugar source to provide corresponding N-acetyllactosamine clusters. Further sugar elongation of the LacNAc clusters was demonstrated using alpha2,3 sialyl transferase and CMP-neuraminic acid (CMP-NANA) to yield the corresponding SiaLacNAc clusters.
authors.affiliation: Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan. koji@fms.saitama-u.ac.jp
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Reina
authors.initials: R
authors.lastname: Kaneko
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Koyama
authors.forename: XiaoTao
authors.initials: X
authors.lastname: Ma
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Esumi
authors.forename: Takemichi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Ken
authors.initials: K
authors.lastname: Hatano
authors.forename: Daiyo
authors.initials: D
authors.lastname: Terunuma
mesh_terms: D000117:Acetylglucosamine / Q000096:biosynthesis / Q000737:chemistry; D000606:Amino Sugars / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D051381:Rats; D012821:Silanes / Q000737:chemistry*; D014531:Uridine Diphosphate Galactose / Q000737:chemistry
chemical_list: D000606:Amino Sugars; D012821:Silanes; C504072:carbosilane; C032233:lactosamine; D014531:Uridine Diphosphate Galactose; C000458:N-acetyllactosamine; D009237:N-Acetyllactosamine Synthase; D000117:Acetylglucosamine