title: The -A162G polymorphism of the PON1 gene and the risk of sporadic amyotrophic lateral sclerosis.
abstract: OBJECTIVE
  Sporadic amyotrophic lateral sclerosis (sALS) is a devastating neurodegenerative disease, which results from complex genetic and environmental interactions. Recent studies have reported an association between several polymorphisms of the PON1 and PON2 genes and risk of sALS. The aim of the present study was to identify an association between the -A162G polymorphism of the promoter region of the human PON1 gene and the risk of sALS in a Polish population.
  
  
  METHODS
  We included 259 patients with a diagnosis of definite or probable sALS (76 bulbar onset, 183 limb onset) and 694 healthy controls matched for age and sex. The diagnosis of ALS was established according to El Escorial criteria. The polymorphism was studied by Single Nucleotide Polymorphism Real-Time Polymerase Chain Reaction analysis.
  
  
  RESULTS
  No overall difference in the PON1 -A162G geno-type and allele distribution was seen between cases and controls (all p > 0.05). There was, however, a difference in the A allele frequency when the bulbar onset group was compared to the controls (p = 0.03), but this significance disappeared after the Bonferroni correction.
  
  
  CONCLUSIONS
  The results did not show that the -A162G polymorphism of the PON1 gene is a risk factor of sALS in a Polish population; it may affect, however, bulbar onset of the disease.
authors.affiliation: Department of Neurology, Jagiellonian University College of Medicine, Krakow. zawislak13@wp.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Zawiślak
authors.forename: Monika
authors.initials: M
authors.lastname: Ostrowska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Golenia
authors.forename: Monika
authors.initials: M
authors.lastname: Marona
authors.forename: Barbara
authors.initials: B
authors.lastname: Tomik
authors.forename: Paweł
authors.initials: P
authors.lastname: Wołkow
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Gryz-Kurek
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szczudlik
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Słowik
mesh_terms: D000328:Adult; D000483:Alleles; D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D043303:Aryldialkylphosphatase / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: D043303:Aryldialkylphosphatase; C468411:PON2 protein, human