Effect of reducing the period of follicle dominance in a timed artificial insemination protocol on reproduction of dairy cows.
Objectives were to determine the effect of reducing the period of follicle dominance in a timed artificial insemination (AI) protocol on pregnancy per AI (P/AI) in Holstein cows. In experiment 1, 165 cows received 2 injections of PGF(2alpha) at 36 and 50 d in milk (DIM). At 61 DIM, cows were assigned randomly to Cosynch 72 h (CoS72: d 61 GnRH, d 68 PGF(2alpha), d 71 GnRH) or to a 5-d Cosynch 72 h with 1 (5dCoS1: d 61 GnRH, d 66 PGF(2alpha), d 69 GnRH) or 2 injections of PGF(2alpha) (5dCoS2: d 61 GnRH, d 66 and 67 PGF(2alpha), d 69 GnRH). Blood was sampled at the first GnRH, first PGF(2alpha), and at the second GnRH of the protocols and assayed for progesterone. Ovulatory responses to GnRH were evaluated by ultrasonography. Cows were considered synchronized if they had concentrations of progesterone >or=1 ng/mL and <1 ng/mL on the days of the PGF(2alpha), and the second GnRH of the protocols, respectively, and if they ovulated within 48 h of the second GnRH injection. In experiment 2, 933 cows were assigned randomly to CoS72 or 5dCoS2. Blood was assayed for progesterone and ovaries were scanned as in experiment 1. Plasma on the days of the first PGF(2alpha) and final GnRH of the timed AI protocols was assayed for estradiol in 75 cows. Pregnancy was diagnosed on d 38 and 66 after AI. In experiment 1, the proportions of cows with corpora lutea (CL) regression on the day of AI differed and were 79.0, 59.1, and 95.7% for CoS72, 5dCoS1, and 5dCoS2, respectively. Cows that ovulated to the first GnRH of the Cosynch tended to have lesser CL regression than cows that did not ovulate (73.0 vs. 86.4%). Protocol synchronization differed between treatments and they were greater for CoS72 (69.4%) and 5dCoS2 (78.4%) than for 5dCoS1 (42.3%). In experiment 2, CL regression was lesser (91.5 vs. 96.3%) but detection of estrus at timed AI (30.9 vs. 23.6%) was greater for CoS72 than 5dCoS2, and cows in estrus had increased P/AI (46.2 vs. 31.9%). Cows in CoS72 ovulated a larger follicle and had greater concentrations of estradiol on the day of AI than cows in 5dCoS2, but protocol synchronization tended to increase in cows receiving the 5dCoS2. When all 933 cows were evaluated, P/AI was greater for 5dCoS2 than for CoS72 (37.9 vs. 30.9%). Similarly, when only cows with progesterone <1 ng/mL on the day of AI were evaluated, P/AI was greater for 5dCoS2 than for CoS72 (39.3 vs. 33.9%). Treatment with PGF(2alpha) on d 5 and 6 after GnRH resulted in increased luteolysis and allowed for reducing the interval from GnRH to timed AI, which increased P/AI. Reducing time of follicle dominance in timed AI protocols improves fertility of lactating dairy cows.
Santos
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JE
Department of Animal Sciences, University of Florida, Gainesville, Florida, USA. jepsantos@ufl.edu
Narciso
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Rivera
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Thatcher
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Chebel
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D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005300:Fertility Agents, Female / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D007774:Lactation / Q000187:drug effects; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects*; D010053:Ovary / Q000000981:diagnostic imaging / Q000187:drug effects; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011897:Random Allocation; D013997:Time Factors; D014463:Ultrasonography
D005300:Fertility Agents, Female; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone