title: Translational repression by deadenylases.
abstract: The CCR4-CAF1-NOT complex is a major cytoplasmic deadenylation complex in yeast and mammals. This complex associates with RNA-binding proteins and microRNAs to repress translation of target mRNAs. We sought to determine how CCR4 and CAF1 participate in repression and control of maternal mRNAs using Xenopus laevis oocytes. We show that Xenopus CCR4 and CAF1 enzymes are active deadenylases and repress translation of an adenylated mRNA. CAF1 also represses translation independent of deadenylation. The deadenylation-independent repression requires a 5' cap structure on the mRNA; however, deadenylation does not. We suggest that mere recruitment of CAF1 is sufficient for repression, independent of deadenylation.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Cooke
authors.forename: Andrew
authors.initials: A
authors.lastname: Prigge
authors.forename: Marvin
authors.initials: M
authors.lastname: Wickens
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D012315:RNA Caps / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D005095:Exoribonucleases; D012315:RNA Caps; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins