title: Targeted metabolomics for biomarker discovery.
abstract: Metabolomics is a truly interdisciplinary field of science, which combines analytical chemistry, platform technology, mass spectrometry, and NMR spectroscopy with sophisticated data analysis. Applied to biomarker discovery, it includes aspects of pathobiochemistry, systems biology/medicine, and molecular diagnostics and requires bioinformatics and multivariate statistics. While successfully established in the screening of inborn errors in neonates, metabolomics is now widely used in the characterization and diagnostic research of an ever increasing number of diseases. In this Review we highlight important technical prerequisites as well as recent developments in metabolomics and metabolomics data analysis with special emphasis on their utility in biomarker identification and qualification, as well as targeted metabolomics by employing high-throughput mass spectrometry.
authors.affiliation: Swansea University, School of Medicine, Institute of Mass Spectrometry, Singleton Park, Swansea SA2 8PP, UK.
authors.forename: William J
authors.initials: WJ
authors.lastname: Griffiths
authors.forename: Therese
authors.initials: T
authors.lastname: Koal
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.forename: Matthias
authors.initials: M
authors.lastname: Kohl
authors.forename: David P
authors.initials: DP
authors.lastname: Enot
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Deigner
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods* / Q000639:trends
chemical_list: D015415:Biomarkers; D008055:Lipids