title: Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells.
abstract: • Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family. • We labelled the tobacco cell line BY-2 and pollen tubes with a fluorescent derivative of phosphatidylcholine and assayed for patterns of fluorescently labelled products. Growth of pollen tubes was analysed. • We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl(3). Investigation of possible metabolic pathways that control DAG generation and consumption during the response to Al showed that DAG originated from the reaction catalysed by PC-PLC. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line. The growth of pollen tubes arrested by Al was rescued by externally added DAG. • Our observation strongly supports the role of DAG generated by PC-PLC in the response of tobacco cells to Al.
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, v. v. i., Rozvojová 263, 165 02 Prague 6, Czech Republic.
authors.forename: Premysl
authors.initials: P
authors.lastname: Pejchar
authors.forename: Martin
authors.initials: M
authors.lastname: Potocký
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Novotná
authors.forename: Stepánka
authors.initials: S
authors.lastname: Veselková
authors.forename: Daniela
authors.initials: D
authors.lastname: Kocourková
authors.forename: Olga
authors.initials: O
authors.lastname: Valentová
authors.forename: Katerina
authors.initials: K
authors.lastname: Schwarzerová
authors.forename: Jan
authors.initials: J
authors.lastname: Martinec
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004075:Diglycerides / Q000096:biosynthesis*; D007477:Ions; D010713:Phosphatidylcholines / Q000378:metabolism*; D053205:Pollen Tube / Q000254:growth & development / Q000378:metabolism; D011162:Porphobilinogen / Q000031:analogs & derivatives / Q000378:metabolism; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: C011439:1,2-diacylglycerol; C538930:3-anilino difluoroboron dipyrromethene; D004075:Diglycerides; D007477:Ions; D010713:Phosphatidylcholines; D011162:Porphobilinogen; D000535:Aluminum; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C