title: Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs.
abstract: Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture.
authors.affiliation: Novartis Vaccines and Diagnostics, Cambridge, MA, USA.
authors.forename: Pirada
authors.initials: P
authors.lastname: Suphaphiphat
authors.forename: Michael
authors.initials: M
authors.lastname: Franti
authors.forename: Armin
authors.initials: A
authors.lastname: Hekele
authors.forename: Anders
authors.initials: A
authors.lastname: Lilja
authors.forename: Terika
authors.initials: T
authors.lastname: Spencer
authors.forename: Ethan
authors.initials: E
authors.lastname: Settembre
authors.forename: Gene
authors.initials: G
authors.lastname: Palmer
authors.forename: Stefania
authors.initials: S
authors.lastname: Crotta
authors.forename: Annunziata B
authors.initials: AB
authors.lastname: Tuccino
authors.forename: Bjoern
authors.initials: B
authors.lastname: Keiner
authors.forename: Heidi
authors.initials: H
authors.lastname: Trusheim
authors.forename: Kara
authors.initials: K
authors.lastname: Balabanis
authors.forename: Melissa
authors.initials: M
authors.lastname: Sackal
authors.forename: Mithra
authors.initials: M
authors.lastname: Rothfeder
authors.forename: Christian W
authors.initials: CW
authors.lastname: Mandl
authors.forename: Philip R
authors.initials: PR
authors.lastname: Dormitzer
authors.forename: Peter W
authors.initials: PW
authors.lastname: Mason
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004196:Disease Outbreaks; D004285:Dogs; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016415:Sequence Alignment; D014779:Virus Replication
chemical_list: C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus