[Study on the counting of Streptococcus mutans, Streptococcus sanguis, Haemophilus actinomycetemcomitans by methyl thiazolyl tetrazolium colorimetric method].
OBJECTIVE
To explore the feasibility of methyl thiazolyl tetrazolium (MTT) colorimetric method and the applied condition for the normal bacteria in the mouth, as Streptococcus mutans (S. mutans), Streptococcus sanguis (S. sanguis), Haemophilus actinomycetemcomitans (H. actinomycetemcomitans).


METHODS
Colony forming units (CFU) which was the standard antitheses was used to count bacteria. This study would gain some parameters by changing wavelength, reactive time, dosage and so on. MTT colorimetric method was applied in the counting of S. mutans, S. sanguis and H. actinomycetemcomitans.


RESULTS
When counting S. mutans, the best wavelength was 510 nm, the best range was 1.5 x 10(5) - 1.0 x 10(7) CFU x mL(-1). When counting S. sanguis, the best wavelength was 545 nm, the best range was 1.5 x 10(5) - 2.0 x 10(7) CFU x mL(-1). When counting H. actinomycetemcomitans, the best wavelength was 557 nm, the best range was 1.0 x 10(6) - 5.0 x 10(7) CFU x mL(-1). MTT colorimetric method can be used for different aged S. mutans, S. sanguis and H. actinomycetemcomitans.


CONCLUSIONS
Oral bacteria could be counted by MTT colorimetric method, which is fast and convenient.
Wang
Zhong-Chao
ZC
Dept. of Endodontics, Stomatological Hospital of Luzhou Medical College, Luzhou 646000, China.
Fan
Li-Yuan
LY
Jiang
Jun-Qiang
JQ
Cai
Wei
W
Ding
Yi
Y
D016976:Aggregatibacter actinomycetemcomitans; D001419:Bacteria; D006801:Humans; D012463:Saliva; D013295:Streptococcus mutans; D013298:Streptococcus sanguis