[Pharmacokinetic evaluation of danshensu with in vivo microdialysis in freely moving rat's jugular vein].
OBJECTIVE
To investigate the pharmacokinetics of Danshensu with in vivo microdialysis in freely moving rat's jugular vein.


METHODS
three days after a microdialysis probe introducer was implanted into the jugular vein, a microdialysis probe was introduced to the blood vessel, and began to sample following a single intravenous injection (40 mg x kg(-1)) or a single oral dose (40 mg x kg(-1)) of Danshensu. All the samples were analyzed with HPLC. The concentration of Danshensu in blood were calculated according to the recovery of microdialysis probe and the concentration in dialysates. Pharmacokinetic parameters were than calculated with the concentration-time curve.


RESULTS
For intravenous administration, t(1/2 zeta) = (69.62 +/- 33.42) min, AUC(0-infinity) = (3416.24 +/- 779.80) min x mg x L(-1), MRT(0-infinity) = (38.15 +/- 8.61) min, and for oral administration, Cmax = (7.42 +/- 3.08) mg x L(-1), tmax = (31.50 +/- 8.57) min, t(1/2 zeta) = (83.25 +/- 37.35) min, AUC(0-infinity) = (793.19 +/- 101.32) min x mg x L(-1), MRT(0-infinity) = (125.89 +/- 58.27) min. The oral bioavailability of Danshensu F = 22.56%.


CONCLUSIONS
In vivo microdialysis in freely moving rat's jugular vein is a useful tool to obtain a complete set of free drug concentrations to determine reliable pharmacokinetic parameters.
Zhang
Hengyi
H
College of Biomedical Engineering & Instrumental Science, Zhejiang University, Hangzhou 310027, China. zhy@mail.bme.zju.edu.cn
Lv
Liang
L
Cong
Linlin
L
D000818:Animals; D019540:Area Under Curve; D007601:Jugular Veins / Q000166:cytology* / Q000378:metabolism*; D007773:Lactates / Q000493:pharmacokinetics*; D008297:Male; D017551:Microdialysis; D009043:Motor Activity; D051381:Rats; D017207:Rats, Sprague-Dawley
D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid