[Expression of SSB gene in rats with early isehemic myocardium and its implications].
OBJECTIVE
To examine the expression of Sjögren's syndrome antigen B (SSB) gene in rats with early ischemic myocardium.


METHODS
Adult healthy Sprague-Dawley rats were randomly divided into four groups (ischemia (MI), non-ischemia (NI), sham-operated (SO) and normal control (NO) groups). The expression of SSB and beta-actin gene in the myocardium was examined by real time PCR at 0 min, 5 min, 15 min, 30 min, 60 min, 120 min after ligation of the left anterior descending coronary artery (LAD).


RESULTS
The expression of SSB gene was down-regulated after ischemia. Significantly lower expression of the gene in the rats with myocardial ischemia was found at 120 min after ligation compared with those before ligation and the control group at 120 min after ligation (P < 0.05 ). However, no significant differences were found between other groups (P > 0.05).


CONCLUSIONS
SSB gene may be involved in the pathophysiologic regulating process in myocardial cells at the early stage of myocardial ischemia in rats.
Chen
Tian-Yi
TY
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Gao
Lin-Bo
LB
Liang
Wei-Bo
WB
Wang
Yan-Yun
YY
Xue
Hui
H
Liu
Yun
Y
Pan
Xin-Min
XM
Fang
Wen-Liang
WL
Zhang
Lu-Shun
LS
Zhang
Lin
L
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D000097585:SS-B Antigen
D000199:Actins; D001324:Autoantigens; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D000097585:SS-B Antigen