An easy-to-use tool for planning and modeling a calorimetric titration.
Isothermal titration calorimetry (ITC) is widely employed to measure thermodynamic properties of binding interactions between two macromolecules or a macromolecule and a small ligand. No labeling of interacting species is required for ITC, but this advantage is offset by potentially material-consuming experimental optimization complicated by an indirect readout of an ITC titration. Here we present a simple, practical, and portable spreadsheet-based tool for planning and modeling an ITC titration experiment accompanied by basic guidelines.
Biswas
Tapan
T
Department of Medicinal Chemistry, University of Michigan, Ann Arbor, 48109, USA. tbiswas@umich.edu
Tsodikov
Oleg V
OV
D002151:Calorimetry / Q000379:methods*; D008962:Models, Theoretical; D012984:Software; D013223:Statistics as Topic / Q000379:methods*