Preparation and characterization of 5-Fluorouracil loaded chitosan microspheres by a two-step solidification method.
Chitosan, as a natural biopolymer, has potential applications in controlling the release of drugs due to its unique natural and physiochemical properties. Conventionally, an emulsion crosslinking method was used to prepare chitosan microspheres. In this study, a novel method of preparing 5-fluorouracil-loaded chitosan microspheres was established by combining the emulsification technique and a two-step solidification method using both glutaraldehyde (GTA) and tripolyphosphate (TPP) as crosslinking agents. The microspheres were characterized by surface morphology, encapsulation efficiency, yield of microspheres and drug release profile in vitro. The optimal chitosan microspheres were achieved by crosslinking chitosan with both TPP (0.1%, pH 4.0) and GTA (1.0%, v/v) with span-80 additive under a crosslinking time of 1 h with TPP and GTA, respectively.
Sun
Yi
Y
Department of Pharmaceutics, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Gu
Lei
L
Gao
Yuan
Y
Gao
Feng
F
D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage*; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations; D005472:Fluorouracil / Q000008:administration & dosage*; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D011122:Polyphosphates / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013678:Technology, Pharmaceutical / Q000379:methods*
D000964:Antimetabolites, Antineoplastic; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D011122:Polyphosphates; D048271:Chitosan; C005692:triphosphoric acid; D005976:Glutaral; D005472:Fluorouracil