Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity.
Ionizing radiation generates oxidative stress, which is thought to be a major cause of aging. Although living organisms are constantly exposed to low levels of radiation, most studies examining the effect of radiation have focused on accelerated aging and diminished life span that result from high-dose radiation. On the other hand, several studies have suggested that low-dose radiation enhances the longevity of Drosophila melanogaster. Therefore, investigation of the biological effects of low-dose radiation could contribute to a more comprehensive understanding of the aging process. In this study, microarray and quantitative real time-PCR were used to measure genome-wide changes in transcript levels in low-dose irradiated fruit flies that showed enhanced longevity. In response to radiation, approximately 13% of the genome exhibited changes in gene expression, and a number of aging-related genes were significantly regulated. These data were compared with quantitative trait loci affecting life-span to identify candidate genes involved in enhanced longevity induced by low-dose radiation. This genome-wide survey revealed novel information about changes in transcript levels in low-dose irradiated flies and identified 39 new candidate genes for molecular markers of extended longevity induced by ionizing radiation. In addition, this study also suggests a mechanism by which low-dose radiation extends longevity.
Seong
Ki Moon
KM
Division of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., Dobong-Gu, Seoul, Korea.
Kim
Cha Soon
CS
Seo
Sun-Won
SW
Jeon
Ha Yeon
HY
Lee
Byung-Sub
BS
Nam
Seon Young
SY
Yang
Kwang Hee
KH
Kim
Ji-Young
JY
Kim
Chong Soon
CS
Min
Kyung-Jin
KJ
Jin
Young-Woo
YW
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology* / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects*; D020869:Gene Expression Profiling; D016678:Genome / Q000528:radiation effects*; D008136:Longevity / Q000235:genetics* / Q000528:radiation effects; D008297:Male; D046228:Microarray Analysis; D018384:Oxidative Stress / Q000235:genetics / Q000528:radiation effects; D016133:Polymerase Chain Reaction / Q000379:methods; D040641:Quantitative Trait Loci; D011839:Radiation, Ionizing