title: MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory.
abstract: Since the early 1980s, mass spectrometry has emerged as a particularly powerful tool for analysis and characterization of proteins in research. Recently, bacteriologists have focused their attention on the use of mass spectrometry (MS) for bacterial identification, especially Matrix Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF). Moreover, recent publications have evaluated MALDI-TOF in microbiology laboratory for routine use. MALDI-TOF-MS is a rapid, precise, and cost-effective method for identification of intact bacteria, compared to conventional phenotypic techniques or molecular biology. Furthermore, it allows identification of bacteria directly from clinical samples (blood cultures for example). The goal of this review was to update recent data concerning routine identification of microorganisms by MALDI-TOF in the clinical microbiology laboratory.
authors.affiliation: Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, France. etienne.carbonnelle@egp.aphp.fr
authors.forename: Etienne
authors.initials: E
authors.lastname: Carbonnelle
authors.forename: Cécile
authors.initials: C
authors.lastname: Mesquita
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bille
authors.forename: Nesrine
authors.initials: N
authors.lastname: Day
authors.forename: Brunhilde
authors.initials: B
authors.lastname: Dauphin
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Beretti
authors.forename: Agnès
authors.initials: A
authors.lastname: Ferroni
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutmann
authors.forename: Xavier
authors.initials: X
authors.lastname: Nassif
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*