A new A431/cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening epidermal growth factor receptor antagonists from Radix sophorae flavescentis.
The intracellular kinase domains of epidermal growth factor receptor (EGFR) in some tumor cells such as human epidermal squamous cells (A(431) cells) are an important target for drug discovery. We have developed a new A(431)/cell membrane chromatography (A(431)/CMC)-online-high performance liquid chromatography/mass spectrometry (HPLC/MS) method for screening EGFR antagonists from medicinal herbs such as traditional Chinese medicines (TCMs). In this study, A(431) cells with high EGFR expression levels were used to prepare cell membrane stationary phase (CMSP) in an A(431)/CMC model. The retention fractions eluted from the CMSP column were enriched onto an ODS pre-column and then switched into an HPLC/MS system by combining a 10 port columns switching valve. The screening results found that oxymatrine and matrine from Radix sophorae flavescentis (RSF) were the targeted components which could act on EGFR in similar manner of gefitinib as a control drug. There was a good relationship of their inhibiting effects on EGFR secretion and A(431) cell growth in vitro. This new A(431)/CMC-online-HPLC/MS method can be applied for screening EGFR antagonists from TCMs such as RSF. It will be a useful method for drug discovery with natural medicinal herbs as a leading compound resource.
Wang
Sicen
S
Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, China.
Sun
Meng
M
Zhang
Yanmin
Y
Du
Hui
H
He
Langchong
L
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology; D001667:Binding, Competitive; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000077156:Gefitinib; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology; D011807:Quinolizines / Q000737:chemistry / Q000494:pharmacology; D015203:Reproducibility of Results; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D047428:Protein Kinase Inhibitors; D011799:Quinazolines; D011807:Quinolizines; C037573:oxymatrine; D066246:ErbB Receptors; D000077156:Gefitinib; D000093842:Matrines