title: Single-molecule dual-beam optical trap analysis of protein structure and function.
abstract: Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Sung
authors.forename: Sivaraj
authors.initials: S
authors.lastname: Sivaramakrishnan
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Dunn
authors.forename: James A
authors.initials: JA
authors.lastname: Spudich
mesh_terms: D009218:Myosins / Q000737:chemistry* / Q000378:metabolism*; D052898:Optical Tweezers
chemical_list: D009218:Myosins