title: Genetic variation of 15 autosomal STR loci in a population sample from Poland.
abstract: Fifteen autosomal STR loci included in AmpFlSTR NGM kit were analyzed in 154 unrelated individuals from Poland. This multiplex kit enables simultaneous amplification of 10 standard STR loci included in AmpFlSTR SGM Plus kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D19S433, TH01, FGA, D21S11 and D18S51) and five new mini- and midi-STR loci (D10S1248, D22S1045, D2S441, D1S1656 and D12S391). Population study was conducted to evaluate usefulness of the loci (especially the five new microsatellite systems) in forensic genetic identification examinations. All 15 markers were found to be in Hardy-Weinberg equilibrium. The combined probability of match for the 15 studied STR loci was 3.998 x 10(-19). The same parameter calculated for five new microsatellite loci equaled 8.83 x 10(-7). Discrimination power was particularly high in case of D1S1656 (0.975) and D12S391 (0.972) STR loci.
authors.affiliation: Institute of Forensic Research, Section of Forensic Genetics, Westerplatte 9, 31-033 Krakow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Parys-Proszek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kupiec
authors.forename: Paulina
authors.initials: P
authors.lastname: Wolańska-Nowak
authors.forename: Wojciech
authors.initials: W
authors.lastname: Branicki
mesh_terms: D000483:Alleles; D053803:Forensic Genetics; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D011044:Poland; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics*; D044465:White People / Q000235:genetics*