title: Multilineage differentiation of muscle-derived stem cells from GFP transgenic mice.
abstract: It is unclear whether green fluorescent protein (GFP) expression is maintained during the course of multilineage differentiation of muscle-derived stem cells (MDSCs). We isolated MDSCs from GFP-transgenic mice and transferred them to chondrogenic, neurogenic or myogenic media. Multilineage differentiation was examined by morphological observation, histological staining, immunocytochemical staining, real-time RT-PCR and Western blot. Both differentiated cells and non-differentiated cells maintained stable GFP expression until the cells exhibited a senescent phenotype. Thus, MDSCs from GFP-transgenic mice have multilineage potential in vitro and that GFP expression does not influence the multilineage potential of MDSCs (or vice versa).
authors.affiliation: Department of Orthopaedics, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China. yechuanchina@hotmail.com
authors.forename: Chuan
authors.initials: C
authors.lastname: Ye
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Li
authors.forename: Zixu
authors.initials: Z
authors.lastname: He
authors.forename: Xu
authors.initials: X
authors.lastname: Nin
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianwen
authors.initials: X
authors.lastname: Shang
authors.forename: Riguang
authors.initials: R
authors.lastname: Liu
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003470:Culture Media / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000166:cytology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins