title: Randomized comparison of different ovarian stimulation regimens for assisted reproductive technology in baboons (Papio anubis).
abstract: OBJECTIVE
  To compare different methods of ovarian stimulation (OS) for assisted reproductive technology in baboons.
  
  
  METHODS
  Prospective randomized study.
  
  
  METHODS
  Institute of primate research.
  
  
  METHODS
  Baboons (n = 10) were randomized into two groups (of five animals each) during three different cycles to compare six protocols of OS.
  
  
  METHODS
  Cycle 1: clomiphene citrate (CC) alone (group CC) versus CC and GnRH agonist (group CC-Ag); cycle 2: recombinant gonadotropins (GON) without GnRH agonist (group GON) versus GON and depot GnRH agonist (group GON-AgDepo-1); cycle 3: GON and depot GnRH agonist (group GON-AgDepo-2) versus GON and daily GnRH agonist in a classic long protocol (group GON-Ag). Oocyte aspiration was performed 34-36 hours after injecting 5,000 IU rhCG, followed by fertilization via intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Number and quality of oocytes retrieved and their fertilization rate.
  
  
  RESULTS
  More metaphase II (MII) oocytes were retrieved using the GON-AgDepo-1 (n = 12; 64% MII), GON-AgDepo-2 (n = 9; 79% MII), GON-Ag (n = 16; 88% MII), and GON (n = 6; 59% MII) protocols compared with the CC (n = 9; 15% MII) and CC-Ag (n = 14; 20% MII) protocols. Fertilization by ICSI varied between 43% and 71%.
  
  
  CONCLUSIONS
  In baboons, long and depot protocols yield similar numbers of MII oocytes; however, depot protocol may be preferable because only one injection of GnRH agonist is needed.
authors.affiliation: Leuven University Fertility Center, University Hospital Gasthuisberg, Leuven, Belgium.
authors.forename: Atunga
authors.initials: A
authors.lastname: Nyachieo
authors.forename: Carl
authors.initials: C
authors.lastname: Spiessens
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Chai
authors.forename: Sophie
authors.initials: S
authors.lastname: Debrock
authors.forename: Jason M
authors.initials: JM
authors.lastname: Mwenda
authors.forename: Thomas M
authors.initials: TM
authors.lastname: d'Hooghe
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000819:agonists; D008297:Male; D008597:Menstrual Cycle / Q000187:drug effects / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods*; D048530:Papio anubis / Q000502:physiology*; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted
chemical_list: D003692:Delayed-Action Preparations; D007987:Gonadotropin-Releasing Hormone