title: Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells.
abstract: The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp cells (hDPCs), because they reside in a low-oxygen environment in vivo. Therefore, we presently examined the effects of hypoxia on the proliferation and differentiation of hDPCs in vitro. hDPCs grown under 3% O(2) showed a significantly higher proliferation rate than those under 21% O(2). Then, we prepared hypoxic cultures of hDPCs from older patients' teeth having inflammation and succeeded in recovering and expanding a small number of hDPCs. These cells were confirmed to have capability for osteo/odontogenic differentiation. Hypoxia suppressed the osteo/odontogenic differentiation of hDPCs in vitro and increased the number of cells expressing STRO-1, an early mesenchymal stem cell marker. This simple method will increase the possibility to obtain living hDPCs from damaged and/or aged tissues, from which it is ordinarily difficult to isolate living stem cells with differentiation capability.
authors.affiliation: Department of Oral and Maxillofacial Science, Gifu University Graduate School of Medicine, Gifu City, Gifu 501-1194, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Iida
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takeda-Kawaguchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kunisada
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tezuka
mesh_terms: D000293:Adolescent; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000368:Aged; D000954:Antigens, Surface / Q000096:biosynthesis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008875:Middle Aged; D015675:Osteocalcin / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis; D000099311:Dentin Sialophosphoprotein
chemical_list: D000954:Antigens, Surface; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C525591:STRO-1 antigen, human