title: New automated and high-throughput quantitative analysis of urinary ketones by multifiber exchange-solid phase microextraction coupled to fast gas chromatography/negative chemical-electron ionization/mass spectrometry.
abstract: The present research is focused on automation, miniaturization, and system interaction with high throughput for multiple and specific Direct Immersion-Solid Phase Microextraction/Fast Gas Chromatography analysis of the urinary ketones. The specific Mass Spectrometry instrumentation, capable of supporting such the automated changeover from Negative Chemical to Electron Ionization mode, as well as the automation of the preparation procedure by new device called MultiFiber Exchange, through change of the fibers, allowed a friendly use of mass spectrometry apparatus with a number of advantages including reduced analyst time and greater reproducibility (2.01-5.32%). The detection limits for the seven ketones were less than 0.004 mg/L. For an innovative powerful meaning in high-throughput routine, the generality of the structurally informative Mass Spectrometry fragmentation patterns together with the chromatographic separation and software automation are also investigated.
authors.affiliation: Occupational Health Division, Department of Public Health, Viale Morgagni 48, University of Florence, 50100 Florence, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Pacenti
authors.forename: Stefano
authors.initials: S
authors.lastname: Dugheri
authors.forename: Pietro
authors.initials: P
authors.lastname: Traldi
authors.forename: Filippo
authors.initials: F
authors.lastname: Degli Esposti
authors.forename: Nicola
authors.initials: N
authors.lastname: Perchiazzi
authors.forename: Elena
authors.initials: E
authors.lastname: Franchi
authors.forename: Massimo
authors.initials: M
authors.lastname: Calamante
authors.forename: Ireneo
authors.initials: I
authors.lastname: Kikic
authors.forename: Paolo
authors.initials: P
authors.lastname: Alessi
authors.forename: Alice
authors.initials: A
authors.lastname: Bonacchi
authors.forename: Edoardo
authors.initials: E
authors.lastname: Salvadori
authors.forename: Giulio
authors.initials: G
authors.lastname: Arcangeli
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cupelli