Dimeric bisbenzimidazoles inhibit the DNA methylation catalyzed by the murine Dnmt3a catalytic domain.
When located in the DNA minor groove, dimeric bisbenzimidazoles DB(n) effectively inhibited in vitro the Dnmt3a catalytic domain (IC₅₀ 5-77 μM). The lowest IC₅₀ value was observed for compound DB(11) with an 11-unit methylene linker joining the bisbenzimidazole fragments. Increased time of incubation of DNA with DB(n) as well as the presence of AT-clusters in DNA enhances the inhibitory effect.
Cherepanova
N A
NA
Chemistry Department, Moscow State University, Moscow, Russian Federation.
Ivanov
A A
AA
Maltseva
D V
DV
Minero
A S
AS
Gromyko
A V
AV
Streltsov
S A
SA
Zhuze
A L
AL
Gromova
E S
ES
D000818:Animals; D006690:Bisbenzimidazole / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D020134:Catalytic Domain; D019175:DNA Methylation / Q000187:drug effects*; D015254:DNA Modification Methylases / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure
D015254:DNA Modification Methylases; D006690:Bisbenzimidazole