title: High throughput analysis of 150 pesticides in fruits and vegetables using QuEChERS and low-pressure gas chromatography-time-of-flight mass spectrometry.
abstract: A higher monitoring rate is highly desirable in the labs, but this goal is typically limited by sample throughput. In this study, we sought to assess the real-world applicability of fast, low-pressure GC-time-of-flight MS (LP-GC/TOFMS) for the identification and quantification of 150 pesticides in tomato, strawberry, potato, orange, and lettuce samples. Buffered and unbuffered versions of QuEChERS (which stands for "quick, easy, cheap, effective, rugged, and safe") using dispersive solid-phase extraction (d-SPE) and disposable pipette extraction (DPX) for clean-up were compared for sample preparation. For clean-up of all sample types, a combination of 150 mg MgSO₄, 50mg primary secondary amine (PSA), 50 mg C₁₈, and 7.5 mg graphitized carbon black (GCB) per mL extract was used. No significant differences were observed in the results between the different sample preparation versions. QuEChERS took < 10 min per individual sample, or < 1 h for two chemists to prepare 32 pre-homogenized samples, and using LP-GC/TOFMS, < 10 min run time and < 15 min cycle time allowed > 32 injections in 8 h. Overall, > 126 analytes gave recoveries (3 spiking levels) in the range of 70-120% with < 20% RSD. The results indicate that LP-GC/TOFMS for GC-amenable analytes matches UHPLC-MS/MS in terms of sample throughput and turnaround time for their routine, concurrent use in the analysis of a wide range of analytes in QuEChERS extracts to achieve reliable quantification and identification of pesticide residues in foods.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
authors.forename: Urairat
authors.initials: U
authors.lastname: Koesukwiwat
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Lehotay
authors.forename: Shui
authors.initials: S
authors.lastname: Miao
authors.forename: Natchanun
authors.initials: N
authors.lastname: Leepipatpiboon
mesh_terms: D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D016014:Linear Models; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues