title: Proteome insights into the symbiotic relationship between a captive colony of Nasutitermes corniger and its hindgut microbiome.
abstract: We analyzed the metaproteome of the bacterial community resident in the hindgut paunch of the wood-feeding 'higher' termite (Nasutitermes) and identified 886 proteins, 197 of which have known enzymatic function. Using these enzymes, we reconstructed complete metabolic pathways revealing carbohydrate transport and metabolism, nitrogen fixation and assimilation, energy production, amino-acid synthesis and significant pyruvate ferredoxin/flavodoxin oxidoreductase protein redundancy. Our results suggest that the activity associated with these enzymes may have more of a role in the symbiotic relationship between the hindgut microbial community and its termite host than activities related to cellulose degradation.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Burnum
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Callister
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.forename: Samuel O
authors.initials: SO
authors.lastname: Purvine
authors.forename: Philip
authors.initials: P
authors.lastname: Hugenholtz
authors.forename: Falk
authors.initials: F
authors.lastname: Warnecke
authors.forename: Rudolf H
authors.initials: RH
authors.lastname: Scheffrahn
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Mary S
authors.initials: MS
authors.lastname: Lipton
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000378:metabolism*; D004064:Digestive System / Q000382:microbiology; D020049:Isoptera / Q000382:microbiology*; D054892:Metagenome; D020543:Proteome; D013559:Symbiosis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome