Adult exposure to sasanguasaponin induces spermatogenic cell apoptosis in vivo through increased oxidative stress in male mice.
Effects of sasanguasaponin (SGS), a compound from defatted seeds of Camellia oleifera, on abnormal spermatogenesis were studied in adult male mice using daily intragastric administration of 100, 200 and 400 mg/kg body weight (BW) for 6 weeks. The sperm counts decreased and the number of abnormal spermatozoa increased in mice treated with SGS at 400 mg/kg dose level. Testicular weight and seminiferous tubular area gradually decreased as dosage increased to 400 mg/kg SGS. Germ cells were rarefied and showed irregular distribution in seminiferous tubules (STs). Apoptosis was pronounced among spermatocytes and spermatids at the 400 mg/kg dose level. Antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxide capacity (T-AOC) markedly decreased and malondialdehyde (MDA) concentration increased in the testes. These results suggest that adult exposure to SGS (400 mg/kg) induces spermatogenic apoptosis through increased oxidative stress in male mice.
Li
Jian
J
Laboratory of Veterinary Anatomy, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Wang
Zixu
Z
Shi
Dazhao
D
Chen
Yaoxing
Y
D000818:Animals; D017209:Apoptosis; D004365:Drugs, Chinese Herbal / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress; D012503:Saponins / Q000633:toxicity*; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
C497504:22-O-angeloylcamelliagenin C-3-O-(glucopyranosyl-1-2)(glucopyranosyl-1-2-O-arabinopyranosyl-1-3-)glucopyranosiduronic acid; D004365:Drugs, Chinese Herbal; D012503:Saponins