Bonghan ducts as possible pathways for cancer metastasis.
OBJECTIVE
The present study has been designed to find a possible new route for the metastasis of cancer cells on the fascia surrounding tumor tissue using a novel technique of trypan blue staining.


METHODS
Tumor tissues were grown in the skin of nude mice after sub-cutaneous inoculation with human lung cancer cells. Trypan blue was recently identified as a dye with specificity for Bonghan ducts (BHDs) and not other tissues, such as blood or lymph vessels or nerves.


RESULTS
We demonstrate that the trypan blue staining technique allows the first visualization of BHDs which are connected to tumor tissues.


CONCLUSIONS
Since BHDs are known to make up a circulatory system corresponding to acupuncture meridians or collaterals, we propose that, in addition to the currently known blood or lymph vessels, BHDs on tumor tissue fascia may be a novel pathway for metastasis.
Yoo
Jung Sun
JS
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, Seoul, Korea.
Kim
Hong Bae
HB
Ogay
Vyacheslav
V
Lee
Byung-Cheon
BC
Ahn
Saeyoung
S
Soh
Kwang-Sup
KS
D000818:Animals; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology*; D016740:Meridians; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D013194:Staining and Labeling; D014343:Trypan Blue / Q000032:analysis
D014343:Trypan Blue