Optimization and scale-up of propionic acid production by propionic acid-tolerant Propionibacterium acidipropionici with glycerol as the carbon source.
Propionic acid production by Propionibacterium acidipropionici with glycerol as sole carbon source was studied in 7-L batch and 10m(3) bioreactor cultures. In batch cultures, propionic acid production increased and specific cell growth rate decreased with increasing glycerol concentrations. Maximum propionic acid production and productivity reached 44.62+/-1.12g/L and 0.20+/-0.0075gL(-1)h(-1)at 220h, respectively, when glycerol was fed at a constant rate of 0.01L/h from 72 to 120h with an initial glycerol concentration of 30g/L. In the 10m(3) bioreactor, maximum propionic acid production reached 47.28+/-0.12g/L at 240h. This glycerol feeding approach may be useful for propionic acid production on an industrial scale.
Zhu
Yunfeng
Y
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
Li
Jianghua
J
Tan
Ming
M
Liu
Long
L
Jiang
Lili
L
Sun
Jun
J
Lee
Pengsoon
P
Du
Guocheng
G
Chen
Jian
J
D000192:Actinomycetales / Q000187:drug effects / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D010865:Pilot Projects; D011422:Propionates / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
D011422:Propionates; D002244:Carbon; C029658:propionic acid; D005990:Glycerol