Direct evidence for postmeiotic transcription during Drosophila melanogaster spermatogenesis.
Extensive gene expression during meiosis is a hallmark of spermatogenesis. Although it was generally accepted that RNA transcription ceases during meiosis, recent observations suggest that some transcription occurs in postmeiosis. To further resolve this issue, we provide direct evidence for the de novo transcription of RNA during the postmeiotic phases. These results strengthen the newly emerging notion that postmeiotic transcription is dynamic and integral to the overall process of spermatogenesis.
Vibranovski
Maria D
MD
Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, 60637, USA.
Chalopin
Domitille S
DS
Lopes
Hedibert F
HF
Long
Manyuan
M
Karr
Timothy L
TL
D000818:Animals; D001976:Bromouracil / Q000031:analogs & derivatives; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D008540:Meiosis; D057054:Molecular Imaging; D012333:RNA, Messenger / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic; D014529:Uridine / Q000031:analogs & derivatives / Q000378:metabolism
D012333:RNA, Messenger; D001976:Bromouracil; C006824:5-bromouridine; D014529:Uridine