Antiviral effect of strictinin on influenza virus replication.
Strictinin, which is a member of the ellagitanin family of hydrolyzable tannins, prevented replication of human, duck and swine influenza A viruses (IAVs) in vitro at non-toxic concentrations. The addition of strictinin at the same time as IAV inoculation to MDCK cells inhibited viral replication in a dose-dependent manner. Strictinin showed 50% inhibitory concentrations for IAVs from 0.09±0.021 to 0.28±0.037μM (mean±S.E.M.) by the focus-forming assay. Treatment of MDCK cells with strictinin before and after viral inoculation resulted in no significant antiviral activity. Further studies showed that strictinin inhibited IAV-induced hemifusion. However, strictinin exhibited no inhibitory effect against receptor binding, sialidase activity. Strictinin also showed an antiviral effect on influenza B virus and human parainfluenza virus type-1 in vitro. The results indicate that strictinin is a useful antiviral agent.
Saha
Repon Kumer
RK
Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Takahashi
Tadanobu
T
Kurebayashi
Yuuki
Y
Fukushima
Keijo
K
Minami
Akira
A
Kinbara
Noriaki
N
Ichitani
Masaki
M
Sagesaka
Yuko M
YM
Suzuki
Takashi
T
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects* / Q000502:physiology; D009981:Influenza B virus / Q000187:drug effects* / Q000502:physiology; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology; D053585:Virus Attachment / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D010636:Phenols; D013634:Tannins; C496786:strictinin; D009439:Neuraminidase