title: Transient and permanent gene transfer into the brain of the teleost fish medaka (Oryzias latipes) using human adenovirus and the Cre-loxP system.
abstract: In this study, we demonstrated that human type-5 adenovirus infected the brain of the teleost fish, medaka (Oryzias latipes), in vivo. Injection of adenoviral vector into the mesencephalic ventricle of medaka larvae induced the expression of reporter genes in some parts of the telencephalon, the periventricular area of the mesencephalon and diencephalon, and the cerebellum. Additionally, the Cre-loxP system works in medaka brains using transgenic medaka carrying a vector containing DsRed2, flanked by loxP sites under control of the beta-actin promoter and downstream promoterless enhanced green fluorescent protein (EGFP). We demonstrated that the presence of green fluorescence depended on injection of adenoviral vector expressing the Cre gene and confirmed that EGFP mRNA was transcribed in the virus-injected larvae.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Suehiro
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Okuyama
authors.forename: Atsuko
authors.initials: A
authors.lastname: Shimada
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Naruse
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takeda
authors.forename: Takeo
authors.initials: T
authors.lastname: Kubo
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takeuchi
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism* / Q000821:virology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D019426:Integrases; D008164:Luminescent Proteins / Q000235:genetics; D009990:Oryzias / Q000033:anatomy & histology / Q000235:genetics* / Q000821:virology*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; C413663:fluorescent protein 583; C045073:Cre recombinase; D019426:Integrases