title: Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids.
abstract: Assays based on real-time PCR (TaqMan) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd.
authors.affiliation: Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK. wendy.monger@fera.gsi.gov.uk
authors.forename: Wendy
authors.initials: W
authors.lastname: Monger
authors.forename: Jenny
authors.initials: J
authors.lastname: Tomlinson
authors.forename: Neil
authors.initials: N
authors.lastname: Booonham
authors.forename: Mojca Virscek
authors.initials: MV
authors.lastname: Marn
authors.forename: Irena Mavric
authors.initials: IM
authors.lastname: Plesko
authors.forename: Valérie
authors.initials: V
authors.lastname: Molinero-Demilly
authors.forename: Xavier
authors.initials: X
authors.lastname: Tassus
authors.forename: Ellis
authors.initials: E
authors.lastname: Meekes
authors.forename: Marcel
authors.initials: M
authors.lastname: Toonen
authors.forename: Lambros
authors.initials: L
authors.lastname: Papayiannis
authors.forename: Zoila
authors.initials: Z
authors.lastname: Perez-Egusquiza
authors.forename: Natasa
authors.initials: N
authors.lastname: Mehle
authors.forename: Claudia
authors.initials: C
authors.lastname: Jansen
authors.forename: Steen Lykke
authors.initials: SL
authors.lastname: Nielsen
mesh_terms: D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D019657:Solanaceae / Q000821:virology*; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral