title: Specific fluorogenic probes for ozone in biological and atmospheric samples.
abstract: Ozone exposure is a growing global health problem, especially in urban areas. While ozone in the stratosphere protects the earth from harmful ultraviolet light, tropospheric or ground-level ozone is toxic and can damage the respiratory tract. It has recently been shown that ozone may be produced endogenously in inflammation and antibacterial responses of the immune system; however, these results have sparked controversy owing to the use of a non-specific colorimetric probe. Here we report the synthesis of fluorescent molecular probes able to unambiguously detect ozone in both biological and atmospheric samples. Unlike other ozone-detection methods, in which interference from different reactive oxygen species is often a problem, these probes are ozone specific. Such probes will prove useful for the study of ozone in environmental science and biology, and so possibly provide some insight into the role of ozone in cells.
authors.affiliation: Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Garner
authors.forename: Claudette M
authors.initials: CM
authors.lastname: St Croix
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Pitt
authors.forename: George D
authors.initials: GD
authors.lastname: Leikauf
authors.forename: Shin
authors.initials: S
authors.lastname: Ando
authors.forename: Kazunori
authors.initials: K
authors.lastname: Koide
mesh_terms: D001272:Atmosphere / Q000032:analysis*; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000379:methods*; D010126:Ozone / Q000032:analysis* / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D012140:Respiratory Tract Diseases / Q000378:metabolism / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D010126:Ozone