Should South Africa be performing nucleic acid testing on HIV enzyme-linked immunosorbent assay-negative samples?
The frequency of acute HIV infection (AHI) among HIV-1 enzyme-linked immunosorbent assay (ELISA)-negative samples received from general hospital patient admissions was assessed. Of 3,005 samples pooled for nucleic acid testing, a prevalence of 0.13% was found. Pooled nucleic acid testing may be feasible for low-cost identification of AHI in high-prevalence settings.
Gous
Natasha
N
Department of Molecular Medicine and Haematology, School of Pathology, University of Witwatersrand, Parktown, Johannesburg 2193, South Africa.
Scott
Lesley
L
Perovic
Olga
O
Venter
Francios
F
Stevens
Wendy
W
D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013019:South Africa / Q000453:epidemiology
D015483:HIV Antibodies; D012367:RNA, Viral