title: A successful haploidentical bone marrow transplantation method in rabbits: perfusion method plus intra-bone marrow-bone marrow transplantation.
abstract: Graft versus host disease (GVHD), rejection, delayed immune reconstitution and infections have been significant hurdles to haploidentical BMT. In order to improve the outcome of the current haploidentical-related BMT, we performed a novel BMT method consisting of the perfusion method (PM) plus intra-bone marrow-bone marrow transplantation (IBM-BMT) in a rabbit model. The percentages of T cells in BMCs harvested by the PM and the conventional aspiration method (AM) were 6% and 14%, respectively (p<0.01). Conversely, the CFU-C counts of BMCs in the PM group were significantly higher than those in the AM group. When the BMCs were transplanted into lethally irradiated offspring rabbits by IBM-BMT, hemopoietic recovery in the PM group was faster than in the AM group. The cumulative incidence of acute GVHD was 25% in the PM group versus 75% in the AM group (p<0.05). In addition, the survival rate was 75% in the PM group versus 33% in the AM group (p<0.05). Thus, the new method is able to provide rapid hemopoiesis, reduce the cumulative incidence of acute GVHD, and achieve a higher survival rate. This novel strategy paves the way for new dimensions in haploidentical BMT.
authors.affiliation: Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
authors.forename: Yunze
authors.initials: Y
authors.lastname: Cui
authors.forename: Shuji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Wenhao
authors.initials: W
authors.lastname: Cui
authors.forename: Kequan
authors.initials: K
authors.lastname: Guo
authors.forename: Yuming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000379:methods; D005240:Feasibility Studies; D006086:Graft vs Host Disease / Q000517:prevention & control; D006410:Hematopoiesis / Q000276:immunology; D006648:Histocompatibility / Q000276:immunology; D006801:Humans; D008297:Male; D023421:Models, Animal; D010477:Perfusion; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology