Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina officinalis L.
The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L. to different dosages of UV-B irradiation was investigated. Results showed that superoxide dimutase (SOD) and peroxidase (POX) increased and then maintained at a relatively stable level when subjected to UV-B irradiation. Catalase (CAT) activity under medium dosage of UV-B irradiation (Muv) and high dosage of UV-B irradiation (Huv) treatments were significantly decreased. Ascorbate peroxidase (APX) activity first remained unaltered and then increased in Huv treatment. In addition, the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis (PAGE). The activities of some SOD isoforms were altered by UV-B. Two new bands (POX V and POX VII) appeared upon exposure to all three UV-B dosages. CAT III activity was increased by low dosage of UV-B irradiation (Luv), whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and Huv. Two bands of APX (APX VI and APX VII) were increased and a new band (APX X) was observed under Huv exposure. H2O2 and thiobarbituric acid reacting substance (TBARS) increased under Muv and Huv treatments. Overall, UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in C. officinalis.
Li
Lixia
L
College of Marine Life Science, Ocean University of China, Qingdao 266003, China. lilixianet@126.com
Zhao
Jiqiang
J
Tang
Xuexi
X
D000975:Antioxidants / Q000378:metabolism*; D056890:Eukaryota / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D055871:Tidal Waves; D014466:Ultraviolet Rays
D000975:Antioxidants; D007527:Isoenzymes; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide