title: HPLC - DAD analysis of lycorine in Amaryllidaceae species.
abstract: Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high-performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut). This method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. (Amaryllidaceae) collected during two different vegetation periods. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate 1 mL min(-1) using diode array detector. Validation procedures showed that the method was specific, accurate and precise. The amount of lycorine in S. sicula samples ranged between 0.10-0.53%, whereas in those of S. lutea and P. maritimum, the range was 0.19-0.40% and 0.05-0.14%, respectively.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ege University, 35100 Bornova-Izmir, Turkey. gulen.irem.kaya@ege.edu.tr
authors.forename: Gulen Irem
authors.initials: GI
authors.lastname: Kaya
authors.forename: Derya
authors.initials: D
authors.lastname: Cicek
authors.forename: Buket
authors.initials: B
authors.lastname: Sarikaya
authors.forename: Mustafa Ali
authors.initials: MA
authors.lastname: Onur
authors.forename: Nehir Unver
authors.initials: NU
authors.lastname: Somer
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019668:Liliaceae / Q000737:chemistry*; D010617:Phenanthridines / Q000737:chemistry*
chemical_list: D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; C015330:lycorine