title: Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.
abstract: The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 A resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.23, b = 124.90, c = 164.80 A. The structure was solved by molecular replacement using the structure of T. brockii SADH (PDB entry 1ykf).
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Carla
authors.initials: C
authors.lastname: Protsko
authors.forename: Claire
authors.initials: C
authors.lastname: Vieille
authors.forename: Maris
authors.initials: M
authors.lastname: Laivenieks
authors.forename: Lata
authors.initials: L
authors.lastname: Prasad
authors.forename: David A R
authors.initials: DA
authors.lastname: Sanders
authors.forename: Louis T J
authors.initials: LT
authors.lastname: Delbaere
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D007532:Isoleucine / Q000235:genetics; D009154:Mutation; D046968:Thermoanaerobacter / Q000201:enzymology*
chemical_list: D007532:Isoleucine; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP)