Myelin ingestion by macrophages promotes their motility and capacity to recruit myeloid cells.
Myelin-laden macrophages reside within the CNS, the CSF and in the CNS-draining lymph nodes during MS and EAE, suggesting migration of these macrophages between these compartments and interaction with other cells. Since chemokines and their receptors are pivotal for leukocyte trafficking, we addressed whether myelin ingestion affects chemotaxis of mouse macrophages in vitro. Myelin ingestion enhanced expression of CCR7 and CXCR3 on macrophages and migration towards CCL21 and CXCL10. Furthermore, myelin-laden macrophages released chemoattractants resulting in enhanced migration of myeloid cells in vitro. Our data demonstrate that myelin-laden macrophages have increased motility and suggest trafficking between anatomical compartments in vivo.
van Zwam
Marloes
M
Department of Immunology, Erasmus MC, Rotterdam, The Netherlands.
Wierenga-Wolf
Annet F
AF
Melief
Marie-José
MJ
Schrijver
Benjamin
B
Laman
Jon D
JD
Boven
Leonie A
LA
D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009186:Myelin Sheath / Q000378:metabolism*; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism*; D054400:Receptors, CCR7 / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000378:metabolism; D018709:Statistics, Nonparametric; D013997:Time Factors
C515371:Ccr7 protein, mouse; C515188:Cxcr3 protein, mouse; D054400:Receptors, CCR7; D054367:Receptors, CXCR3