The activation potential of MOF is constrained for dosage compensation.
The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5' or 3' enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.
Prestel
Matthias
M
Adolf-Butenandt-Institute and Centre for Integrated Protein Science, Ludwig-Maximilians-University, 80336 Munich, Germany.
Feller
Christian
C
Straub
Tobias
T
Mitlöhner
Heike
H
Becker
Peter B
PB
D000107:Acetylation; D000818:Animals; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012737:Sex Factors; D015533:Transcriptional Activation; D014960:X Chromosome / Q000378:metabolism
D029721:Drosophila Proteins; D009687:Nuclear Proteins; D051548:Histone Acetyltransferases; C111063:mof protein, Drosophila