Detection of six genetically modified maize lines using optical thin-film biosensor chips.
As more and more genetically modified organisms (GMO) are commercialized, efficient and inexpensive assays are required for their quick detection. An event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. This study developed a system for detecting six GM maize lines (Bt11, Bt176, GA21, MON810, NK603, and T25) using optical silicon thin-film biosensor chips. Aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip surface. Biotinylated PCR amplicons were then hybridized with the probes. After washing and brief incubation with an anti-biotin IgG horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate, biotinylated PCR amplicons perfectly matched with the probes can be visualized by the color change on the chip surface (gold to blue/purple). This assay is extremely robust, exhibits high sensitivity and specificity, and is flexible from low through moderate to high throughput.
Bai
Sulan
S
College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.
Zhang
Jie
J
Li
Shucheng
S
Chen
Haodong
H
Terzaghi
William
W
Zhang
Xin
X
Chi
Xiurong
X
Tian
Jin
J
Luo
Hongxia
H
Huang
Wensheng
W
Chen
Ying
Y
Zhang
Yaochuan
Y
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*