title: [Pharmacokinetic evaluation of danshensu with in vivo microdialysis in freely moving rat's jugular vein].
abstract: OBJECTIVE
  To investigate the pharmacokinetics of Danshensu with in vivo microdialysis in freely moving rat's jugular vein.
  
  
  METHODS
  three days after a microdialysis probe introducer was implanted into the jugular vein, a microdialysis probe was introduced to the blood vessel, and began to sample following a single intravenous injection (40 mg x kg(-1)) or a single oral dose (40 mg x kg(-1)) of Danshensu. All the samples were analyzed with HPLC. The concentration of Danshensu in blood were calculated according to the recovery of microdialysis probe and the concentration in dialysates. Pharmacokinetic parameters were than calculated with the concentration-time curve.
  
  
  RESULTS
  For intravenous administration, t(1/2 zeta) = (69.62 +/- 33.42) min, AUC(0-infinity) = (3416.24 +/- 779.80) min x mg x L(-1), MRT(0-infinity) = (38.15 +/- 8.61) min, and for oral administration, Cmax = (7.42 +/- 3.08) mg x L(-1), tmax = (31.50 +/- 8.57) min, t(1/2 zeta) = (83.25 +/- 37.35) min, AUC(0-infinity) = (793.19 +/- 101.32) min x mg x L(-1), MRT(0-infinity) = (125.89 +/- 58.27) min. The oral bioavailability of Danshensu F = 22.56%.
  
  
  CONCLUSIONS
  In vivo microdialysis in freely moving rat's jugular vein is a useful tool to obtain a complete set of free drug concentrations to determine reliable pharmacokinetic parameters.
authors.affiliation: College of Biomedical Engineering & Instrumental Science, Zhejiang University, Hangzhou 310027, China. zhy@mail.bme.zju.edu.cn
authors.forename: Hengyi
authors.initials: H
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Lv
authors.forename: Linlin
authors.initials: L
authors.lastname: Cong
mesh_terms: D000818:Animals; D019540:Area Under Curve; D007601:Jugular Veins / Q000166:cytology* / Q000378:metabolism*; D007773:Lactates / Q000493:pharmacokinetics*; D008297:Male; D017551:Microdialysis; D009043:Motor Activity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid