title: Effects of sizes and conformations of fish-scale collagen peptides on facial skin qualities and transdermal penetration efficiency.
abstract: Fish-scale collagen peptides (FSCPs) were prepared using a given combination of proteases to hydrolyze tilapia (Oreochromis sp.) scales. FSCPs were determined to stimulate fibroblast cells proliferation and procollagen synthesis in a time- and dose-dependent manner. The transdermal penetration capabilities of the fractionationed FSCPs were evaluated using the Franz-type diffusion cell model. The heavier FSCPs, 3500 and 4500 Da, showed higher cumulative penetration capability as opposed to the lighter FSCPs, 2000 and 1300 Da. In addition, the heavier seemed to preserve favorable coiled structures comparing to the lighter that presents mainly as linear under confocal scanning laser microscopy. FSCPs, particularly the heavier, were concluded to efficiently penetrate stratum corneum to epidermis and dermis, activate fibroblasts, and accelerate collagen synthesis. The heavier outweighs the lighter in transdermal penetration likely as a result of preserving the given desired structure feature.
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Huey-Jine
authors.initials: HJ
authors.lastname: Chai
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Li
authors.forename: Han-Ning
authors.initials: HN
authors.lastname: Huang
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Li
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Chan
authors.forename: Chyuan-Yuan
authors.initials: CY
authors.lastname: Shiau
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000328:Adult; D000825:Animal Structures / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D020405:Dermis / Q000187:drug effects / Q000502:physiology; D004058:Diffusion / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005399:Fishes / Q000033:anatomy & histology*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D011487:Protein Conformation; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D012869:Skin Absorption / Q000187:drug effects*; D055815:Young Adult
chemical_list: D010455:Peptides; D003094:Collagen; D016650:Fluorescein-5-isothiocyanate