Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate.
With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer-ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules.
Gopinath
Subash C B
SC
National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
Kumaresan
Ramanujam
R
Awazu
Koichi
K
Fujimaki
Makoto
M
Mizuhata
Minoru
M
Tominaga
Junji
J
Kumar
Penmetcha K R
PK
D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009696:Nucleic Acids / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D009696:Nucleic Acids; D011506:Proteins; D006046:Gold; D012825:Silicon