title: LC-MS/MS studies of ritonavir and its forced degradation products.
abstract: Forced degradation of ritonavir (RTV), under the conditions of hydrolysis (acidic, basic and neutral), oxidation, photolysis and thermal stress as prescribed by ICH was studied using LC-MS/MS. Eight degradation products were formed and their separation was accomplished on Waters XTerra C(18) column (250 mm x 4.6 mm i.d., 5 microm) using water:methanol:acetonitrile as (40:20:40, v/v/v) mobile phase in an isocratic elution mode by LC. The method was extended to LC-MS/MS for characterization of the degradation products and the pathways of decomposition were proposed. No previous reports were found in the literature regarding the characterization of degradation products of ritonavir.
authors.affiliation: Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: B
authors.initials: B
authors.lastname: Ramachandra
authors.forename: R Mastan
authors.initials: RM
authors.lastname: Vali
authors.forename: S Satyanarayana
authors.initials: SS
authors.lastname: Raju
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D017320:HIV Protease Inhibitors / Q000032:analysis* / Q000737:chemistry; D006868:Hydrolysis; D019438:Ritonavir / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017320:HIV Protease Inhibitors; D019438:Ritonavir