GCF and serum myeloperoxidase and matrix metalloproteinase-13 levels in renal transplant patients.
OBJECTIVE
The rationale of this study was to address whether local or systemic changes reflect proteolytic (matrix metalloproteinase-13) or oxidative (myeloperoxidase) stress in renal transplant patients receiving cyclosporine-A (CsA) and having gingival overgrowth (GO), in patients receiving CsA therapy and having no GO and patients receiving tacrolimus therapy.


METHODS
Gingival crevicular fluid (GCF) samples were collected from sites with (GO+) and without GO (GO-) in CsA patients having GO; GO- sites in CsA patients having no GO; sites from tacrolimus, gingivitis and healthy subjects. GCF and serum myeloperoxidase (MPO) and matrix metalloproteinase-13 (MMP-13) levels were determined by ELISA.


RESULTS
GO+ sites in CsA patients having GO had elevated GCF MPO levels than those of CsA patients having no GO, tacrolimus and healthy subjects (p<0.005), but comparable to those of gingivitis. GCF MPO levels were higher in GO+ compared to GO- sites in CsA patients having GO (p<0.05). Patient groups had similar, but higher GCF MMP-13 levels than healthy group.


CONCLUSIONS
These results show that CsA and tacrolimus therapy have not a significant effect on GCF MPO and MMP-13 levels, and gingival inflammation seems to be the main reason for their elevations.
Emingil
Gülnur
G
Ege University, School of Dentistry, Department of Periodontology, Bornova, 35100 Izmir, Turkey. gemingil@yahoo.com
Afacan
Beral
B
Tervahartiala
Taina
T
Töz
Hüseyin
H
Atilla
Gül
G
Sorsa
Timo
T
D000328:Adult; D016022:Case-Control Studies; D016572:Cyclosporine / Q000009:adverse effects* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D019214:Gingival Overgrowth / Q000097:blood / Q000139:chemically induced* / Q000201:enzymology; D005891:Gingivitis / Q000201:enzymology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects* / Q000097:blood; D016030:Kidney Transplantation; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D016011:Normal Distribution; D009195:Peroxidase / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D018709:Statistics, Nonparametric; D016559:Tacrolimus / Q000097:blood / Q000627:therapeutic use; D055815:Young Adult
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D009195:Peroxidase; D053509:Matrix Metalloproteinase 13; D016559:Tacrolimus