title: [Histopathological evaluation of bone regeneration using human resorbable demineralized membrane].
abstract: OBJECTIVE
  Filling a bone defect with bone substitution materials is a therapy of choice, but the infiltration of connective tissue from the mucoperiostal flap may compromise a healing of bone substitutions with bony wall defects. Application of membrane as a barrier is indicated as a solution to this problem. The aim of this study was to show a pathohistological view of bone regeneration and the significance of human resorbable demineralized membrane (HRDM), 200 microns thick in bone regeneration regarding mandibular defects in an experiment on dogs.
  
  
  METHODS
  The experiment was performed on six dogs. Bone defects were created in all six dogs on the right side of the mandible after the elevation of the mucoperiostal flap. One defect was filled with human deproteinised bone (HDB), and in between HDB and soft tissue RHDM of 200 microns thick was placed. In the second defect, used as a control one, only HDB without RHDM was placed. Two dogs were sacrificed two months after the surgery, another two dogs four months after the surgery and the last two dogs six months after the surgery. After that, samples of bone tissue were taken for histopathological analysis.
  
  
  RESULTS
  In all the six dogs with defects treated with HDB and RHDM the level of bone regeneration was much higher in comparison with the control defects without RHDM.
  
  
  CONCLUSIONS
  Membrane, as a cover of bony defect, is useful and benefits bone regeneration. Bony de fects covered with RHDM show better bony healing despite the fact that bone regeneration was not fully complete for as long as six months after the RHDM implantation.
authors.affiliation: Vojnomedicinska akademija, Klinika za maksilofacijalnu, oralnu hirurgiju i implantologiju, Beograd, Srbija.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Tatić
authors.forename: Novak
authors.initials: N
authors.lastname: Stamatović
authors.forename: Marija
authors.initials: M
authors.lastname: Bubalo
authors.forename: Snezana
authors.initials: S
authors.lastname: Jancić
authors.forename: Alek
authors.initials: A
authors.lastname: Racić
authors.forename: Nikola
authors.initials: N
authors.lastname: Miković
authors.forename: Natalija
authors.initials: N
authors.lastname: Tatić
authors.forename: Mia
authors.initials: M
authors.lastname: Rakić
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D004285:Dogs; D006801:Humans; D008334:Mandible / Q000473:pathology / Q000601:surgery*
chemical_list: D001672:Biocompatible Materials