[Biomimetic synthesis of a novel antibacterial nano-composite materials of hydroxyapatite and gelatin for bone repair and its biocompatibility in vitro].
OBJECTIVE
To evaluate the bioactivity of a new type of antibacterial nano-composite materials of low-crystallinity hydroxyapatite and gelatin for bone repair in vitro.


METHODS
The nanocomposite was prepared by a biomimetic method. A certain amount of acidic solution containing hydroxyapatite and minocycline was added into gelatin solution at 40 degrees centigrade and pH 7-8 to obtain HA-Gel composite containing anti-bacterial drug minocycline. The material was co-cultured with rat bone marrow stromal cells(BMSCs) in vitro.


RESULTS
The results indicated that the nanoscopic low-crystallinity hydroxyapatite was distributed evenly in the fibrils of the composite by controlling crystal growth. The HA-Gel nanocomposite had complicated porous structure. It could promote BMSCs adhesion, proliferation, and differentiation in vitro.


CONCLUSIONS
The BMSCs response to the material considerably shows that it is bioactive. The nanocomposite may be promising as a kind of materials for bone replacement and a carrier for controlled-release therapeutic agents.
Dou
Xiao-chen
XC
Department of Stomatology, Anhui Medical University, Hefei 230032, China. 56461190@qq.com
Li
Quan-li
QL
Zhou
Jian
J
Tang
Jian
J
D000818:Animals; D000900:Anti-Bacterial Agents; D032701:Biomimetics; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D017886:Durapatite; D005780:Gelatin; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D000900:Anti-Bacterial Agents; D005780:Gelatin; D017886:Durapatite