[Genetic diversity of eukaryotic picoplankton of eight lakes in Nanjing].
The method of terminal restriction fragment length polymorphism (T-RFLP) was used to study the genetic diversity of eukaryotic picoplankton (0.2-5.0 microm) in the pelagic and littoral zones in 8 lakes with different trophic status in Nanjing. The objectives of this study were to confirm the difference of the genetic diversity of eukaryotic picoplankton among lakes and the main factors affecting this difference. T-RFLP indicated that there were various fingerprints among lakes and zones. The average terminal restriction fragments (T-RFs) in the littoral and pelagic zones were 16.4 and 15.9, respectively. The littoral zone in Lake Nan and the pelagic zone in Lake Mochou had 30 T-RFs and 27 T-RFs, respectively. The T-RFs were the least abundant (10) in the pelagic zone in Lake Baijia with relatively low trophic status. The genetic diversity of eukaryotic picoplankton was higher in the littoral zone than that in the pelagic zone except Lake Pipa and Mochou. The cluster analysis indicated that the similarities of the littoral zones and the pelagic zones were very high except Lake Baijia, Qian and Nan. The canonical correspondence analysis between the genetic diversity of eukaryotic picoplankton and environmental factors revealed the concentration of chlorophyll a had the most important impact on the eukaryotic picoplankton communities (p = 0.004). The results indicated that the genetic diversity of eukaryotic picoplankton is affected by the trophic status and has the difference in the pelagic and littoral zones.
Zhao
Bi-ying
BY
College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. zby5843@hotmail.com
Chen
Mei-jun
MJ
Sun
Ying
Y
Chen
Fei-zhou
FZ
Yang
Jia-xin
JX
D002681:China; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D005618:Fresh Water / Q000032:analysis*; D014644:Genetic Variation; D010933:Plankton / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014871:Water Microbiology
D002734:Chlorophyll; D000077194:Chlorophyll A