Selective fluorescence detection of cysteine and N-terminal cysteine peptide residues.
A new fluorogenic fluorescein derivative containing an alpha,beta-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.
Lim
Soojin
S
Department of Chemistry, Portland State University, Portland, Oregon 97207, USA.
Escobedo
Jorge O
JO
Lowry
Mark
M
Xu
Xiangyang
X
Strongin
Robert
R
D000447:Aldehydes / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000447:Aldehydes; D010455:Peptides; D003545:Cysteine