title: High-speed atomic force microscopy techniques for observing dynamic biomolecular processes.
abstract: Atomic force microscopy (AFM) enables direct visualization of single-protein molecules in liquids at submolecular resolution. High-speed AFM further makes it possible to visualize dynamic biomolecular processes at subsecond resolution. However, dynamic imaging of biomolecular processes imposes various requirements on "wet techniques" and imaging conditions, which are often different from those for static imaging. This chapter first surveys the imposed requirements, then focuses on practical techniques associated with dynamic imaging, highlighting the preparation of substrate surfaces, and presents examples of the use of these techniques.
authors.affiliation: Department of Physics, Kanazawa University, Kakuma-machi, Kanazawa, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamamoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Uchihashi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kodera
authors.forename: Hayato
authors.initials: H
authors.lastname: Yamashita
authors.forename: Shingo
authors.initials: S
authors.lastname: Nishikori
authors.forename: Teru
authors.initials: T
authors.lastname: Ogura
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Shibata
authors.forename: Toshio
authors.initials: T
authors.lastname: Ando
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D001436:Bacteriorhodopsins / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000538:Aluminum Silicates; D001436:Bacteriorhodopsins; D019809:Streptavidin; C011934:mica