Skin incision induces expression of axonal regeneration-related genes in adult rat spinal sensory neurons.
Skin incision and nerve injury both induce painful conditions. Incisional and postsurgical pain is believed to arise primarily from inflammation of tissue and the subsequent sensitization of peripheral and central neurons. The role of axonal regeneration-related processes in development of pain has only been considered when there has been injury to the peripheral nerve itself, even though tissue damage likely induces injury of resident axons. We sought to determine if skin incision would affect expression of regeneration-related genes such as activating transcription factor 3 (ATF3) in dorsal root ganglion (DRG) neurons. ATF3 is absent from DRG neurons of the normal adult rodent, but is induced by injury of peripheral nerves and modulates the regenerative capacity of axons. Image analysis of immunolabeled DRG sections revealed that skin incision led to an increase in the number of DRG neurons expressing ATF3. RT-PCR indicated that other regeneration-associated genes (galanin, GAP-43, Gadd45a) were also increased, further suggesting an injury-like response in DRG neurons. Our finding that injury of skin can induce expression of neuronal injury/regeneration-associated genes may impact how clinical postsurgical pain is investigated and treated.


CONCLUSIONS
Tissue injury, even without direct nerve injury, may induce a state of enhanced growth capacity in sensory neurons. Axonal regeneration-associated processes should be considered alongside nerve signal conduction and inflammatory/sensitization processes as possible mechanisms contributing to pain, particularly the transition from acute to chronic pain.
Hill
Caitlin E
CE
University of Miami, The Miami Project to Cure Paralysis, Miami, Florida, USA.
Harrison
Benjamin J
BJ
Rau
Kris K
KK
Hougland
M Tyler
MT
Bunge
Mary Bartlett
MB
Mendell
Lorne M
LM
Petruska
Jeffrey C
JC
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000473:pathology*; D062109:Dermatologic Surgical Procedures; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D009416:Nerve Regeneration / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D012867:Skin / Q000293:injuries*; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology / Q000601:surgery*; D014158:Transcription, Genetic