Chemotherapy-induced myelotoxicity and incidence of lung metastasis in an animal model.
We examined how chemotherapy-induced myelotoxicity and the associated leukopaenia affect cancer metastasis in an animal model. Myelotoxicity was induced by a single injection of 5-fluorouracil (5-FU) or Cisplatin, administered to 7-week-old BALB/c mice. CT-26 murine colon carcinoma cells were injected into the lateral tail vein on days 0, 1, 3, 5, and 7 after anticancer drug injection. On day 14 after cancer cell injection, the number of pulmonary colonies was measured in a double-blind setting. Compared with Cisplatin, 5-FU induced severe leukopaenia and bone marrow suppression, while on day 5, both drugs induced severe myelotoxicity. The number of pulmonary colonies did not correlate with the severity of leukopaenia, regardless of the type or time of drug injection, except in the group pretreated with Cisplatin (3 days prior to cancer cell injection). Our results suggest that chemotherapy-induced myelotoxicity does not increase the incidence of cancer metastasis in this animal model.
Son
Jin-Young
JY
Liver and Immunology Research Center, Institute of Traditional Medicine and Bioscience of Daejeon University, Daejeon, Republic of Korea.
Shin
Jang-Woo
JW
Wang
Jing-Hua
JH
Park
Hye-Jung
HJ
Kim
Hyeong-Geug
HG
Raghavendran
Hanumantha Rao Balaji
HR
Son
Chang-Gue
CG
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000633:toxicity*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D005472:Fluorouracil / Q000633:toxicity*; D007970:Leukopenia / Q000139:chemically induced / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D002945:Cisplatin; D005472:Fluorouracil