A correlation between polyomavirus JC virus quantification and genotypes in renal transplantation.
OBJECTIVE
To determine whether the John Cunningham virus (JCV) viral load and the multigenotypes in viruria are correlated with transplant patients.


METHODS
The urine of 60 renal transplant patients and 60 healthy controls were screened. We used quantitative real-time polymerase chain reaction and capillary electrophoresis to assess viral load and genotype respectively.


RESULTS
The incidence of viruria and viral load were higher in transplant patients with P = .0092 and P = .0094, respectively. The incidence of different genotype in transplant patients versus controls was 8.3% versus 13.3% for single genotype, 26.7% versus 5% for 2 genotypes, and 5% versus 0% for multigenotypes (P = .0004). The incidence of more than 2 genotypes was high in people with a high viral load and closely related with the transplant patients (P = .007).


CONCLUSIONS
Not only viral load but also genotypes are important as a screening parameter to understand the immune milieu of the patients to prevent subsequent complications like polyomavirus nephropathy, infection, and malignancy.
Yin
Wen-Yao
WY
Department of General Surgery, Buddhist Dalin Tzu Chi General Hospital, 2 Min-Sheng Road, Dalin town, Chia-Yi, Taiwan. yaowen@mail.tcu.edu.tw
Lu
Ming-Chi
MC
Lee
Ming-Che
MC
Liu
Su-Chin
SC
Lin
Teng-Yi
TY
Lai
Ning-Sheng
NS
D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics / Q000652:urine*; D005260:Female; D005838:Genotype; D006801:Humans; D007165:Immunosuppression Therapy; D015994:Incidence; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D027601:Polyomavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D019562:Viral Load
D004279:DNA, Viral