title: Allele distributions and frequencies of the six prion protein gene (PRNP) polymorphisms in Asian native cattle, Japanese breeds, and mythun (Bos frontalis).
abstract: Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun x cattle composite animals. At the 23-bp indel site, a deletion (23-) allele was a major allele in all populations except mythun. At the 12-bp indel site, an insertion (12+) allele was a major allele in all populations. The 14-bp indel site was polymorphic in all Asian native cattle. In the octapeptide repeat region, a six-repeat allele was a major allele in all populations, and 5/5 and 4/6 genotypes were detected in Japanese Black and Mongolian cattle and in mythun, respectively. Two nonsynonymous single nucleotide polymorphisms (SNPs) (K3T and S154N) were detected in Asian native cattle and mythun. Haplotype analysis using the genotypes of the six sites estimated 33 different haplotypes. The haplotype 23- 12- K 6 S 14+ was found in all populations.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan, simogiri@agri.kagoshima-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimogiri
authors.forename: George
authors.initials: G
authors.lastname: Msalya
authors.forename: Si Lhyam
authors.initials: SL
authors.lastname: Myint
authors.forename: Shin
authors.initials: S
authors.lastname: Okamoto
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kawabe
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Tanaka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Mannen
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Minezawa
authors.forename: Takao
authors.initials: T
authors.lastname: Namikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Amano
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yoshizane
authors.initials: Y
authors.lastname: Maeda
mesh_terms: D000818:Animals; D002417:Cattle; D005787:Gene Frequency; D006239:Haplotypes; D006824:Hybridization, Genetic; D007438:Introns / Q000235:genetics; D009842:Oligopeptides / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D011328:Prions / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D009842:Oligopeptides; D011328:Prions