title: Activation of fibroblast and papilla cells by glycolipid biosurfactants, mannosylerythritol lipids.
abstract: Mannosylerythritol lipids (MELs), the extracellular glycolipids produced from feedstock by yeasts belonging to the genus Pseudozyma, are the most promising biosurfactants known due to its versatile interfacial and biochemical actions. In order to broaden the application in cosmetics, the cell activating property of MELs was investigated using cultured fibroblast and papilla cells, and a three-dimensional cultured human skin model. The di-acetylated MEL (MEL-A) produced from soybean oil significantly increased the viability of the fibroblast cells over 150% compared with that of control cells. On the other hand, no cell activation was observed by the treatment with MEL-A produced from olive oil. The mono-acetylated MEL (MEL-B) hardly increased the cell viability. The viability of the fibroblast cells decreased with the addition of more than 1 microg/L of MELs, whereas the cultured human skin cells showed high viability with 5 microg/L of MELs. Interestingly, the papilla cells were dramatically activated with 0.001 microg/L of MEL-A produced from soybean oil: the cell viability reached at 150% compared with that of control cells. Consequently, the present MEL-A produced from soybean oil should have a potential as a new hair growth agent stimulating the papilla cells.
authors.affiliation: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Masaru
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Michiko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sogabe
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D000107:Acetylation; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006017:Glycolipids / Q000737:chemistry / Q000494:pharmacology*; D018859:Hair Follicle / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid