[Induction of apoptosis and related genes by five kinds of dental materials on L929 cell].
OBJECTIVE
To evaluate a new type of diatomite-based machinable ceramic biocompatibility by studying its induced apoptosis on L929 cell in contrasted with other prosthodontics materials.


METHODS
Cell line was treated with extracting liquid containing different concentrations of diatomite-based machinable ceramic and other materials. Flow cytometry tested cell cycle progression and induced cell apoptosis. Annexin V-FITC/PI apoptosis staining kit quantitative detected cell death patterns. The expression of Bcl-2 and Bax mRNA were determined by reverse transcription-polymerase chain reaction.


RESULTS
The experimental groups had no special influence on cell cycle. Apoptosis rates of the new ceramic closed to the negative group (P > 0.05). The apoptosis rate of resin was the highest, and the cell necrosis level of resin was increased, which had significant difference to the new ceramic (P < 0.05). The Bcl-2 and Bax mRNA levels of the new ceramic and the negative group were closed to each other, which had no statistical significance (P > 0.05).


CONCLUSIONS
The new diatomite-based machinable ceramic has no apparent cytotoxicity, which is consistent with the clinical application of the basic requirements of biocompatibility.
Wang
Xue
X
Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Zhang
Fei-Min
FM
Liu
Mei
M
Yin
Xue-Meng
XM
Gu
Ning
N
Guang
Han-Bing
HB
D000818:Animals; D017304:Annexin A5; D017209:Apoptosis; D002460:Cell Line; D003764:Dental Materials; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D051379:Mice; D009336:Necrosis
D017304:Annexin A5; D003764:Dental Materials; C492760:FITC-annexin A5; D016650:Fluorescein-5-isothiocyanate