title: Skin-derived mesenchymal stem cells (S-MSCs) induce endothelial cell activation by paracrine mechanisms.
abstract: The mesenchymal stem cells (MSCs) are able to accumulate at the site of tissue damage. For this reason, they must transmigrate across the endothelium. In this study, we focused on skin-derived MSCs (S-MSCs), because the skin represents a useful stem cell source, and we analysed the VEGF released by S-MSCs, because it is known to promote endothelial cell proliferation and vascular permeability. Moreover, we evaluated the influence of S-MSC-conditioned medium on human aortic endothelial cell intracellular calcium concentration ([Ca(2+)](i)) and nitric oxide (NO) production, given their important role in endothelial permeability modulation. Our results suggest that human S-MSCs may interact with the endothelium via paracrine mechanisms, probably leading to an alteration of the endothelial barrier. Consequently, we could hypothesize that a therapeutic approach based on human skin-derived MSCs may have a positive effect on tissue repair.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Salvolini
authors.forename: Monia
authors.initials: M
authors.lastname: Orciani
authors.forename: Arianna
authors.initials: A
authors.lastname: Vignini
authors.forename: Monica
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.forename: Laura
authors.initials: L
authors.lastname: Mazzanti
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Primio
mesh_terms: D000328:Adult; D001011:Aorta / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019899:Paracrine Communication; D012867:Skin / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide; D002118:Calcium