Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans.
We compared the performance of loop-mediated isothermal amplification (LAMP) with that of a multiplex PCR method for differential detection of human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, with no false positives, showed a higher sensitivity (88.4%) than the multiplex PCR (37.2%). Thus, it is expected that the LAMP method has a high value for molecular diagnosis of taeniasis.
Nkouawa
Agathe
A
Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Hokkaido, Japan.
Sako
Yasuhito
Y
Li
Tiaoying
T
Chen
Xingwang
X
Wandra
Toni
T
Swastika
I Kadek
IK
Nakao
Minoru
M
Yanagida
Tetsuya
T
Nakaya
Kazuhiro
K
Qiu
Dongchuan
D
Ito
Akira
A
D000818:Animals; D005243:Feces / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D012680:Sensitivity and Specificity; D013621:Taenia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology*