[Search for protective antigens in Ixodes persulcatus (ixodidae) salivary gland extracts].
RT-PCR evaluation of the activity of eight Ixodes persulcatus salivary gland genes shows clear distinctions in their expression depending of the stage of tick feeding. Out of them, only Salp 10 and Salp 15 proteins may be regarded as candidates for protective antigens to develop anti-tick and anti-Borrelia vaccines. Firstly they play an important role in feeding a tick and modifying a host's immune response. Secondly, the increasing expression of the salp 10 and salp 10 genes begins at early tick feeding stages. Thirdly, the activity of these genes increases with the beginning of feeding by tens and hundreds times and keeps at this level until the third tick feeding stage is over.
Shtannikov
A V
AV
Reshetniak
T V
TV
Repolovskaia
T V
TV
Panfertsev
E A
EA
Perovskaia
O N
ON
Gutova
V P
VP
Vasil'eva
I S
IS
Ershova
A S
AS
Prilipov
A G
AG
Biketov
S F
SF
Zeidner
N
N
D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D016056:Immunodominant Epitopes / Q000235:genetics*; D018884:Ixodes / Q000235:genetics* / Q000502:physiology; D008193:Lyme Disease / Q000517:prevention & control; D022123:Lyme Disease Vaccines / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D017778:Vaccines, Combined / Q000235:genetics
D016056:Immunodominant Epitopes; D022123:Lyme Disease Vaccines; D012471:Salivary Proteins and Peptides; D017778:Vaccines, Combined