[Changes of bacterial community structure on reusing domestic sewage of Daoxianghujing Hotel to landscape water].
A 16S rDNA library was used to evaluate the bacterial diversity and identify dominant groups of bacteria in different treatment pools in the domestic sewage system of the Beijing Daoxianghujing Hotel. The results revealed that there were many types of bacteria in the hotel domestic sewage, and the bacterial Shannon-Weaver diversity index was 3.12. In addition, epsilon Proteobacteria was found to be the dominant group with the ratio of 32%. In addition, both the CFB phylum, Fusobacteria, gamma Proteobacteria and Firmicutes were also reached to 9%-15%. After treated with the reclaimed water station, the bacterial Shannon-Weaver diversity index was reduced to 2. 41 and beta Proteobacteria became the dominant group and occupied 73% of the total clones. However, following artificial wetland training, the bacterial Shannon-Weaver diversity index in the sample increased to 3.38, Actinobacteria arrived to 33% and became the most dominant group; Cyanobacteria reached to 26%, and was the second dominant group. But, the control sample comprised 38% Cyanobacteria, and mainly involved in Cyanobium, Synechoccus and Microcystis, with ratios of 47.1%, 17.6% and 8.8%, respectively. Some bacteria of Microcystis aenruginosa were also detected, which probably resulted in the light bloom finally. Therefore, the bacterial diversity and community structures changed in response to treatment of the hotel domestic sewage; there was no cyanobacteria bloom explosion in the treated water. This study will aid in investigation the changes of microbial ecology in different types of water and providing the useful information for enhancing the cyanobacteria blooms control from ecological angle.
Zhu
Jing-nan
JN
College of Life Science, Capital Normal University, Beijing 100048, China. zhujn84ah@yahoo.com.cn
Wang
Xiao-dan
XD
Zhai
Zhen-hua
ZH
Ma
Wen-lin
WL
Li
Rong-qi
RQ
Wang
Xue-lian
XL
Li
Yan-hong
YH
D039903:Actinobacteria / Q000254:growth & development / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D002681:China; D000458:Cyanobacteria / Q000254:growth & development / Q000302:isolation & purification; D011157:Population Dynamics; D020560:Proteobacteria / Q000254:growth & development* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology
D012336:RNA, Ribosomal, 16S; D012722:Sewage