Root cadmium desorption methods and their evaluation with compartmental modeling.
• Desorption of plant roots is often employed in studies of plant physiology and nutrition; however, there have been few studies on the validity of desorption procedures. • Branched and in-line kinetic models with five compartments - cadmium (Cd)-chelate, Cd(2+), root apoplast, root symplast and vacuole - were developed to evaluate the efficacy of diethylenetriaminepentaacetic acid (DTPA) and CaCl(2) methods for the desorption of Cd from roots of durum wheat seedlings. Solution Cd(2+) could exchange with apoplast and symplast Cd simultaneously in the branched model and sequentially in the in-line model. • A 10-min desorption with 1 × 10(-6 )M DTPA at room temperature or cold (0°C) 5 × 10(-3) M CaCl(2) was required to achieve 99% recovery of apoplast-bound (109)Cd when experimental results were interpreted with the branched model. However, when the same data sets were analysed with the in-line model, only partial desorption was achieved. Arguments are presented that suggest that the branched model is correct. • It is suggested that compartmental modeling is a suitable tool for the study of plant root uptake and desorption kinetics, and that there are advantages over more commonly used calculation procedures.
Buckley
Wayne T
WT
Brandon Research Centre, Agriculture and Agri-Food Canada, Brandon, MB, Canada. wayne.buckley@agr.gc.ca
Buckley
Katherine E
KE
Huang
Jianzhong John
JJ
D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism*; D002451:Cell Compartmentation; D007700:Kinetics; D008954:Models, Biological; D010827:Physiology / Q000379:methods*; D018517:Plant Roots / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*
D002104:Cadmium