Release of genotype 1 hepatitis E virus from cultured hepatoma and polarized intestinal cells depends on open reading frame 3 protein and requires an intact PXXP motif.
Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3 (ORF3) protein, and a proline-rich sequence in ORF3 was important for egress from cultured cells and for infection of macaques. Both intracellular ORF3 protein accumulation and virus release occurred at the apical membrane of polarized Caco-2 cells. ORF3 protein and lipids were intimately associated with virus particles produced in either cell line; ORF2 epitopes were masked in these particles and could not be immunoprecipitated with anti-ORF2.
Emerson
Suzanne U
SU
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive, MSC 8009, Bethesda, MD 20892, USA. semerson@niaid.nih.gov
Nguyen
Hanh T
HT
Torian
Udana
U
Burke
Danielle
D
Engle
Ronald
R
Purcell
Robert H
RH
D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D016751:Hepatitis E / Q000821:virology; D016752:Hepatitis E virus / Q000472:pathogenicity / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D008253:Macaca mulatta; D008992:Monkey Diseases; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D057074:Virus Release
C400973:ORF3 protein, Hepatitis E virus; D014764:Viral Proteins