A mouse model to study infection against porcine circovirus type 2: viral distribution and lesions in mouse.
BACKGROUND
Little information is known about viral distribution and transmission of porcine circovirus type 2 (PCV2) in species other than swine. It is still a debated topic whether the PCV2 could be infected and caused clinical lesions. Our study is aimed to estimate the susceptibility of Kunming mouse to PCV2. Forty-eight, 6-week-old Kunming mice were randomly divided into four groups. Group A (C1-C12) was inoculated with PK-15 cell culture as a control group. Group B (sPCV1-12) was inoculated orally and intramuscularly with PCV2 (10(6.2)TCID50/ml). Group C (mPCV1-12) was inoculated orally and intramuscularly with PCV2 (10(6.2)TCID50/ml) and a booster inoculation at days 14 and 28 after the first inoculation. Group D (MixPCV1-12) was unvaccinated but released into Group C. Each group was sacrificed at 7, 14, 28, and 42 days post-inoculation, respectively. Necropsy was checked on every mouse. Sera samples were collected for the test of PCV2 specific antibody. Tissues were collected for histopathology study and polymerase chain reaction (PCR).


RESULTS
The results showed that viral replication, seroconversion, and microscopic lesions were found in inoculated mice. Continuous existence of PCV2 viruses in lymph nodes have been confirmed by PCR, which took at least seven days for the virus to be transferred into other organs from the primary interface, and the diffusion to thymus had been retarded for seven days. Special PCV2 antibody could be found in PCV2 inoculation mice and was significantly higher than that in the control. Further more, microscopic lesions and the main target of PCV2 focused in the lymph nodes with a characteristic depletion and occasional necrosis of lymphocytes in the cortex and paracortex were found in inoculated mice.


CONCLUSIONS
The Kunming mouse could be infected by PCV2 virus and used as a PCV2 infected experimental model.
Li
Jun
J
College of Life Sciences, Key Laboratory of Animal Resistance of Shandong Province, Shandong Normal University, Jinan, 250014, China.
Yuan
Xiaoyuan
X
Zhang
Chaofan
C
Miao
Lanfei
L
Wu
Jiaqiang
J
Shi
Jianli
J
Xu
Shaojian
S
Cui
Shangjin
S
Wang
Jinbao
J
Ai
Hongbin
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018173:Circoviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D004195:Disease Models, Animal; D008168:Lung / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D013553:Swine Diseases / Q000276:immunology / Q000473:pathology* / Q000821:virology
D000914:Antibodies, Viral