Reduction of ischemic and oxidative damage to the hypothalamus by hyperbaric oxygen in heatstroke mice.
The aims of the present paper were to ascertain whether the heat-induced ischemia and oxidative damage to the hypothalamus and lethality in mice could be ameliorated by hyperbaric oxygen therapy. When normobaric air-treated mice underwent heat treatment, the fractional survival and core temperature at 4 hours after heat stress were found to be 0 of 12 and 34 degrees C +/- 0.3 degrees C, respectively. In hyperbaric oxygen-treated mice, when exposed to the same treatment, both fractional survival and core temperature values were significantly increased to new values of 12/12 and 37.3 degrees C +/- 0.3 degrees C, respectively. Compared to normobaric air-treated heatstroke mice, hyperbaric oxygen-treated mice displayed lower hypothalamic values of cellular ischemia and damage markers, prooxidant enzymes, proinflammatory cytokines, inducible nitric oxide synthase-dependent nitric oxide, and neuronal damage score. The data indicate that hyperbaric oxygen may improve outcomes of heatstroke by normalization of hypothalamic and thermoregulatory function in mice.
Tai
Po-An
PA
Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan.
Chang
Chen-Kuei
CK
Niu
Ko-Chi
KC
Lin
Mao-Tsun
MT
Chiu
Wen-Ta
WT
Lin
Jia-Wei
JW
D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D018883:Heat Stroke / Q000150:complications* / Q000473:pathology / Q000628:therapy*; D006931:Hyperbaric Oxygenation; D007031:Hypothalamus / Q000201:enzymology / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress; D016019:Survival Analysis
D016207:Cytokines; D018836:Inflammation Mediators; D052247:Nitric Oxide Synthase Type II