beta-aminobutyric acid primes an NADPH oxidase-dependent reactive oxygen species production during grapevine-triggered immunity.
The molecular mechanisms underlying the process of priming are poorly understood. In the present study, we investigated the early signaling events triggered by beta-aminobutyric acid (BABA), a well-known priming-mediated plant resistance inducer. Our results indicate that, in contrast to oligogalacturonides (OG), BABA does not elicit typical defense-related early signaling events nor defense-gene expression in grapevine. However, in OG-elicited cells pretreated with BABA, production of reactive oxygen species (ROS) and expression of the respiratory-burst oxidase homolog RbohD gene were primed. In response to the causal agent of downy mildew Plasmopara viticola, a stronger ROS production was specifically observed in BABA-treated leaves. This process was correlated with an increased resistance. The NADPH oxidase inhibitor diphenylene iodonium (DPI) abolished this primed ROS production and reduced the BABA-induced resistance (BABA-IR). These results suggest that priming of an NADPH oxidase-dependent ROS production contributes to BABA-IR in the Vitis-Plasmopara pathosystem.
Dubreuil-Maurizi
Carole
C
Universite de Bourgogne, Dijon, France.
Trouvelot
Sophie
S
Frettinger
Patrick
P
Pugin
Alain
A
Wendehenne
David
D
Poinssot
Benoît
B
D000613:Aminobutyrates / Q000494:pharmacology*; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D002118:Calcium / Q000378:metabolism; D017931:DNA Primers; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D019255:NADPH Oxidases / Q000378:metabolism*; D010838:Phytophthora / Q000276:immunology / Q000472:pathogenicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000382:microbiology; D027843:Vitis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology
D000613:Aminobutyrates; D017931:DNA Primers; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D002118:Calcium