title: Dynamic changes in functional gene copy numbers and microbial communities during degradation of pyrene in soils.
abstract: This study investigates the dynamics of pyrene degradation rates, microbial communities, and functional gene copy numbers during the incubation of pyrene-spiked soils. Spiking pyrene to the soil was found to have negligible effects on the bacterial community present. Our results demonstrated that there was a significant difference in nidA gene copy numbers between sampling dates in QZ soil. Mycobacterium 16S rDNA clone libraries showed that more than 90% mycobacteria detected were closely related to fast-growing PAH-degrading Mycobacterium in pyrene-spiked soil, while other sequences related to slow-growing Mycobacterium were only detected in the control soil. It is suggested that nidA gene copy number and fast-growing PAH-degrading Mycobacterium could be used as indicators to predict pyrene contamination and its degradation activity in soils.
authors.affiliation: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Peng
authors.forename: Chao
authors.initials: C
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: Qiao
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yong-Guan
authors.initials: YG
authors.lastname: Zhu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D018628:Gene Dosage / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial; D009161:Mycobacterium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011721:Pyrenes / Q000378:metabolism / Q000633:toxicity*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D011721:Pyrenes; D012989:Soil Pollutants; C030984:pyrene