title: Serotypes and susceptibilities of paediatric clinical isolates of Streptococcus pneumoniae in Crete, Greece, before and after the heptavalent pneumococcal conjugate vaccine.
abstract: All Streptococcus pneumoniae strains isolated from paediatric clinical samples at Heraklion University General Hospital in the 10-year period 2000-2009 were tested for serotype and susceptibility to antimicrobials. Among a total of 258 strains, 159 were isolated in the 5-year period 2000-2004, before the introduction of the heptavalent pneumococcal conjugate vaccine (PCV7), and 99 in the post-PCV7 5-year period 2005-2009. The prevalence of PCV7-included serotypes decreased in the post-PCV7 period (p = 0.0002), but an increase was observed for serotypes 7F (p = 0.002) and 19A (p = 0.004). Pan-susceptibility rates and susceptibility to cotrimoxazole increased in the post-PCV7 period (p = 0.01 and p = 0.008, respectively), but serotype 19A emerged as a contributor to multi-resistance (p = 0.007). PCV7 was followed by decreased S. pneumoniae resistance and prevalence of vaccine-related serotypes but increased prevalence of serotypes 7F and 19A. Continuing surveillance is required after the recent introduction of PCV10 and PCV13.
authors.affiliation: Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographic Medicine, University Hospital of Heraklion, Heraklion, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Maraki
authors.forename: G
authors.initials: G
authors.lastname: Samonis
authors.forename: E
authors.initials: E
authors.lastname: Galanakis
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D002701:Chloramphenicol / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D006115:Greece; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D049674:History, 21st Century; D006801:Humans; D017589:Immunization Programs; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D022242:Pneumococcal Vaccines; D015363:Quinolones / Q000494:pharmacology; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification; D013752:Tetracycline / Q000494:pharmacology; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology; D018074:Vaccines, Conjugate / Q000276:immunology; D014640:Vancomycin / Q000494:pharmacology; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D018942:Macrolides; D022242:Pneumococcal Vaccines; D015363:Quinolones; D018074:Vaccines, Conjugate; D047090:beta-Lactams; D002981:Clindamycin; D002701:Chloramphenicol; D014640:Vancomycin; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D013752:Tetracycline