title: [Ultrastructure and TUNEL staining on inhibition of Rubus alceaefolius total alkaloids for apoptosis of liver in rat models of acute hepatitis].
abstract: OBJECTIVE
  To investigate the anti-apoptosis effects of Rubus alceaefolius total alkaloids in rats with hepatic injury.
  
  
  METHODS
  The hepatic injury model of rat was induced by intraperitoneal injection with CCl4. Sixty SD rats were randomly divided into the normal group, the model group, the R. alceaefolius total alkaloids intervened group, and the bifendate intervened group. The expressions of the levels of liver cell apoptosis were determined by TUNEL. Ultrastructures of the liver cells were observed with transmission electron microscope.
  
  
  RESULTS
  Compared with the model group, the degree of hepatic injury and the positive expressions of apoptosis in liver tissues in the R. alceaefolius total alkaloids intervened groups and the bifendate intervened group were significantly lower.
  
  
  CONCLUSIONS
  R. alceaefolius total alkaloids could reduce the pathological changes and degree of hepatic injury in rats, which may be partially through inhibiting the expressions of apoptosis in liver tissue.
authors.affiliation: Key Laboratory of Basics of Integrative Medicine of Fujian Higher Education, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China. chen.wl@126.com
authors.forename: Wenlie
authors.initials: W
authors.lastname: Chen
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Hong
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Li
authors.forename: Jinyan
authors.initials: J
authors.lastname: Zhao
authors.forename: Jiumaol
authors.initials: J
authors.lastname: Lin
authors.forename: Jianheng
authors.initials: J
authors.lastname: Zhou
authors.forename: Meiyau
authors.initials: M
authors.lastname: Huang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D006520:Hepatitis, Animal / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019685:Rosales / Q000737:chemistry*
chemical_list: D000470:Alkaloids