Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein.
In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L. japonicus and Mesorhizobium loti. When TARF was silenced by virus-induced gene silencing (VIGS) method, TMV RNA accumulation as well as the number of foci formed by GFP-tagged TMV increased drastically. Transient overexpression of TARF reduced the accumulation of TMV. Moreover, TARF transcription was rapidly upregulated by the inoculation of TMV in tobacco plants. These results indicated that TARF is a RING finger protein that inhibits the accumulation of TMV via the interaction of TMV RdRp.
Yamaji
Yasuyuki
Y
Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ayyamaji@mail.ecc.u-tokyo.ac.jp
Hamada
Koji
K
Yoshinuma
Toshio
T
Sakurai
Keitaro
K
Yoshii
Atsushi
A
Shimizu
Takumi
T
Hashimoto
Masayoshi
M
Suzuki
Masashi
M
Namba
Shigetou
S
Hibi
Tadaaki
T
D054884:Host-Pathogen Interactions; D000070116:Lotus / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000276:immunology* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development / Q000276:immunology*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
D010940:Plant Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase