title: Molecular comparison of Quebec and Newfoundland populations of the blackfly, Simulium vittatum, species complex.
abstract: Specific haplotypes at five positions in the COI and COII mitochondrial genes allowed a partial differentiation of Simulium vittatum Zetterstedt (Diptera: Simuliidae) populations from Quebec-Ontario and Newfoundland, respectively. This geographical signature was superimposed on about 40 other polymorphic sites such that sequence divergence alone did not enable a clear-cut distinction between the two populations. Together with the sporadic occurrence of haplotypes intermediate to the Newfoundland and Quebec-Ontario consensus, this suggested that one peculiar sequence among many found in populations from the North American landmass predominates in Newfoundland as a result of a founder effect. The internal transcribed spacer (ITS1) sequence from the nuclear rDNA transcription unit was no more able to resolve populations along geographical lines than the COI/COII criteria.
authors.affiliation: Département de Chimie-biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Quebec G9A 5H7, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Gaudreau
authors.forename: B
authors.initials: B
authors.lastname: LaRue
authors.forename: G
authors.initials: G
authors.lastname: Charpentier
mesh_terms: D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D008969:Molecular Sequence Data; D009523:Newfoundland and Labrador; D011792:Quebec; D012843:Simuliidae / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV