title: Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus.
abstract: A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in five of 390 A. fumigatus isolates screened. Analysis of purified dsRNA revealed four distinct species 3560, 3159, 3006 and 2863 base pairs in length (dsRNAs 1-4) which were cloned and sequenced. Each dsRNA contains a single open reading frame (ORF) with short 5' and 3' untranslated regions containing strictly conserved termini. The deduced 1114 amino acid (aa) protein (molecular mass=128 kDa) encoded by the dsRNA1 ORF showed homology to the RNA-dependent RNA polymerase (RdRP) of viruses belonging to the Chrysoviridae. Eight motifs characteristic of RdRPs were identified. The dsRNA2 ORF encodes the putative coat protein subunit (953aa; molecular mass=107 kDa). The dsRNA3 and dsRNA4 ORFs respectively encode putative proteins (891aa, molecular mass=99 kDa) and (847aa, molecular mass=95 kDa), both of which have significant similarity to proteins encoded by comparable chrysovirus dsRNAs. The dsRNA profile, amino acid sequence alignments, and phylogenetic analyses all indicate that AfuCV is a new species within the family Chrysoviridae.
authors.affiliation: Division of Biology, Faculty of Natural Sciences Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, United Kingdom.
authors.forename: Atif
authors.initials: A
authors.lastname: Jamal
authors.forename: Elaine M
authors.initials: EM
authors.lastname: Bignell
authors.forename: Robert H A
authors.initials: RH
authors.lastname: Coutts
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase