[The effects of hydroxyethyl starch on lipopolysaccharide-induced endothelial injury in rat mesenteric venules].
OBJECTIVE
To investigate the effect of hydroxyethyl starch (HES, 130/0.4) on lipopolysaccharide (LPS)-induced endothelial injury in rat mesenteric venules.


METHODS
Thirty-six Wistar rats weighing 200 - 250 g were randomly divided into 3 groups, n = 12 for each group. Rats were given LPS (2 mg/kg) and followed by normal saline 16 ml x kg(-1) x h(-1) in LPS group or HES 16 ml x kg(-1) x h(-1) in HES group for 60 min. Rats in control group received equal volume of saline. The dynamic changes in diameters of postcapillary venules, red blood cell velocities in venules, and hydrogen peroxide released from venules were evaluated. The ultrastructure of postcapillary venule was observed by electron microscopy.


RESULTS
Mesenteric venular RBC velocities, vascular diameters and shear rates on the venular wall remained constant in any of the groups during the observation (P > 0.05). The hydrogen peroxide generation from venules increased in both HES and LPS groups (P < 0.05). In HES group, the hydrogen peroxide generation and cytoplasmic caveolae in postcapillary venule induced by LPS were significantly inhibited compared with LPS group (P < 0.05).


CONCLUSIONS
Hydroxyethyl starch (130/0.4) could reduce the LPS-induced endothelial injury in rat mesentery. The effect was related to the inhibition of hydrogen peroxide generation on venular wall.
Yao
Tong
T
Department of Anesthesiology, First Hospital of Peking University, Beijing, China. yaotong21cn@hotmail.com
Liu
Yu-ying
YY
Hu
Bai-he
BH
Chang
Xin
X
Wu
Xin-min
XM
Han
Jing-yan
JY
D000818:Animals; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D008643:Mesentery / Q000098:blood supply; D051381:Rats; D017208:Rats, Wistar; D014699:Venules / Q000187:drug effects*
D006895:Hydroxyethyl Starch Derivatives; D008070:Lipopolysaccharides