Expression of heat shock proteins in osteosarcomas.
OBJECTIVE
Heat shock proteins (HSPs) protect cells against stress-associated injuries and are overexpressed in several malignant tumours. We investigated the potential roles of HSP27, HSP60, and HSP70 in conventional and low grade central osteosarcoma.


METHODS
Expressions of HSP27, HSP60, and HSP70 were analysed using immunohistochemistry on tissue sections from 52 cases of conventional osteosarcoma and 21 cases of low grade central osteosarcoma. We evaluated the expression of each protein and examined its relationship with clinicopathological parameters.


RESULTS
We found significantly different expressions of HSP27 and HSP70 between conventional and low grade central osteosarcoma [34.6% versus 4.8% (p = 0.008), 88.5% versus 14.3% (p < 0.001)]. However, HSP60 was highly expressed in both kinds of osteosarcoma (92.3% versus 85.7%). In conventional osteosarcoma, a higher expression of HSP27 was significantly related to distant metastasis (p = 0.034) and histological subtype [osteoblastic versus non-osteoblastic (p = 0.041)]. The expressions of HSP60 and HSP70 were not significantly related to any tested clinicopathological parameter.


CONCLUSIONS
HSP27 and HSP70 may be used as differential markers to distinguish conventional and low grade central osteosarcoma. HSP27 may be used as a possible prognostic marker in conventional osteosarcoma cases.
Moon
Ahrim
A
Department of Pathology, School of Medicine, Kyung Hee University, Seoul, Korea.
Bacchini
Patrizia
P
Bertoni
Franco
F
Olvi
Liliana G
LG
Santini-Araujo
Eduardo
E
Kim
Youn Wha
YW
Park
Yong-Koo
YK
D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism* / Q000401:mortality; D002648:Child; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D011379:Prognosis; D056910:Republic of Korea / Q000453:epidemiology; D015996:Survival Rate; D055815:Young Adult
D014408:Biomarkers, Tumor; D006360:Heat-Shock Proteins