[Blood monocytic L-arginine metabolic changes in diabetic foot syndrome].
An inhibition test was used to study mechanisms responsible for L-arginine metabolic disturbances in the blood monocytes of patients with diabetic foot syndrome (DFS). It showed enhanced baseline iNOS activity and inhibition of the arginase pathway with lower nitrite production in response to the administration of lipopolysaccharide in the monocytes of patients with DFS. Impaired L-arginine metabolism was related to the higher activities of protein kinase C (PKC), phosphodiesterase (PDE), and 5-lipoxygenase (5-LO) along with decreased cyclooxygenase activity and drastic protein kinase A (PKA) inhibition. Within the first week, no changes in the wound process were associated with persistent metabolic disturbances of arachidonic acid and serine-threonine kinases with the higher sensitivity of AT1 receptors. In patients with DFS, the condition for wound process termination was decreased baseline iNOS activity and enhanced arginase-1 activity during PKA stimulation with the lower activity of 5-LO, PDE, and PKS. However, impaired mechanisms in the regulation of monocytic L-arginine metabolism persisted even a month later, which predetermines skin remodeling disturbance and the likelihood of recurrent DFS
Barinov
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EF
Sulaeva
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ON
Barinova
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ME
D000328:Adult; D000368:Aged; D001094:Arachidonate 5-Lipoxygenase / Q000097:blood; D016718:Arachidonic Acid / Q000097:blood; D001119:Arginase / Q000097:blood; D001120:Arginine / Q000097:blood*; D017719:Diabetic Foot / Q000097:blood* / Q000473:pathology; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000097:blood; D010727:Phosphoric Diester Hydrolases / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D017346:Protein Serine-Threonine Kinases / Q000097:blood; D044140:Receptor, Angiotensin, Type 1 / Q000097:blood
D008070:Lipopolysaccharides; D044140:Receptor, Angiotensin, Type 1; D016718:Arachidonic Acid; D001120:Arginine; D001094:Arachidonate 5-Lipoxygenase; D052247:Nitric Oxide Synthase Type II; D011451:Prostaglandin-Endoperoxide Synthases; D017346:Protein Serine-Threonine Kinases; D010727:Phosphoric Diester Hydrolases; D001119:Arginase