Study on construction of nano tPA plasmid to prevent thrombosis after mechanical valve replacement in dogs.
BACKGROUND
Mechanical valve is inclined to induce thrombosis. We intend to elucidate the transfection of nano tissue-type plasminogen activator (tPA) gene plasmid to prevent thrombosis after tricuspid mechanical valve replacement in dogs.


METHODS
A dog model of mechanical tricuspid valve replacement was constructed. A constructed chitosan nano tPA gene plasmid was used to transfect the dog cardiocytes at the same time of tricuspid valve replacement. The effect of this gene on the survival of dogs, anticoagulation (prothrombin time, INR and D-dimer contents, thrombosis in heart), and the expressions of tPA were observed.


RESULTS
Nano tPA gene plasmid was successfully constructed and transfected into cardiocytes. The gene could significantly increase the survival of animals and the contents of D-dimer (P < 0.01), and could prevent thrombus formation on mechanical valves, but there was no significant difference of prothrombin time and INR between two groups (P > 0.05).


CONCLUSIONS
The constructed nano tPA gene plasmid could prevent thrombus formation after mechanical valve replacement, which provides a clue for clinical use.
Ji
Shangyi
S
Shenzhen Sun Yat-sen Cardiovascular Hospital, Shenzhen Heart Institute, Guangdong, China. gelinggeli@hotmail.com
Jun
Ji
J
Xiaohan
Yang
Y
Xia
He
H
Xiaolei
Wang
W
Xiaoqing
Ye
Y
Jianzhou
Guo
G
Wenping
Ling
L
Yanhui
Zhang
Z
D000818:Animals; D004285:Dogs; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D006350:Heart Valve Prosthesis / Q000009:adverse effects*; D008297:Male; D023421:Models, Animal; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D053758:Nanoparticles; D010957:Plasmids / Q000235:genetics*; D011517:Prothrombin Time; D013927:Thrombosis / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D010959:Tissue Plasminogen Activator / Q000235:genetics* / Q000378:metabolism / Q000627:therapeutic use; D014162:Transfection; D014261:Tricuspid Valve / Q000601:surgery*
D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; D010959:Tissue Plasminogen Activator