title: Fluorescence in situ hybridization analysis with a tissue microarray: 'FISH and chips' analysis of pathology archives.
abstract: Practicing pathologists expect major somatic genetic changes in cancers, because the morphological deviations in the cancers they diagnose are so great that the somatic genetic changes to direct these phenotypes of tumors are supposed to be correspondingly tremendous. Several lines of evidence, especially lines generated by high-throughput genomic sequencing and genome-wide analyses of cancer DNAs are verifying their preoccupations. This article reviews a comprehensive morphological approach to pathology archives that consists of fluorescence in situ hybridization with bacterial artificial chromosome (BAC) probes and screening with tissue microarrays to detect structural changes in chromosomes (copy number alterations and rearrangements) in specimens of human solid tumors. The potential of this approach in the attempt to provide individually tailored medical practice, especially in terms of cancer therapy, is discussed.
authors.affiliation: Department of Pathology, Hamamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ward, Hamamatsu 431-3192, Japan. hsugimur@hama-med.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mori
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Nagura
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kiyose
authors.forename: Hong
authors.initials: H
authors.lastname: Tao
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Masaru
authors.initials: M
authors.lastname: Isozaki
authors.forename: Hisaki
authors.initials: H
authors.lastname: Igarashi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shinmura
authors.forename: Akio
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kitayama
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Tanioka
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009369:Neoplasms / Q000235:genetics*; D046888:Tissue Array Analysis