title: Pseudo-template molecularly imprinted polymer for selective screening of trace beta-lactam antibiotics in river and tap water.
abstract: To assess the potential risks associated with the environmental exposure of beta-lactam antibiotics (BLAs), the monitoring of the occurrence, distribution, and fate of these emerging contaminants in the environment is required. Herein, we demonstrate a molecularly imprinted solid-phase extraction (MISPE) method for selective and reliable screening of trace BLAs in river and tap water. By developing a low-temperature photopolymerization, highly selective molecularly imprinted polymers (MIPs) for five BLAs (penicillin G, amoxicillin, ampicillin, nafcillin and mezlocillin) were synthesized. Nafcillin was chosen as a pseudo template to make the MIP sorbent (Nafc-MIP), which was used in pseudo-template MISPE for preconcentration of the other four BLAs from river and tap water. The application of pseudo-template MISPE overcomes the template bleeding, which significantly elevates the sample background and restricts the application of MIP for detection of the target BLA below 2 microg/L. The average recoveries of BLAs are in the range of 60-90% when Nafc-MIP was adopted as the selective MISPE sorbent. The developed method was validated, and applied to the screening of trace beta-lactam antibiotics in river and tap water. The linearity of the calibration curve for each BLA was observed over the range of 0.1-20 microg/L (r>0.998). The beta-lactam antibiotics were found within the range of 0-9.56 microg/L in river water at the downstream of antibiotics manufacturers, and none were detected in the tap water.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Junfa
authors.initials: J
authors.lastname: Yin
authors.forename: Zihui
authors.initials: Z
authors.lastname: Meng
authors.forename: Mingjun
authors.initials: M
authors.lastname: Du
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.forename: Maoyong
authors.initials: M
authors.lastname: Song
authors.forename: Hailin
authors.initials: H
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid; D016014:Linear Models; D054802:Molecular Imprinting; D010406:Penicillins / Q000737:chemistry / Q000302:isolation & purification*; D010777:Photochemistry / Q000379:methods; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014881:Water Supply / Q000032:analysis*
chemical_list: D010406:Penicillins