[Leukemia inhibitory factor suppresses renal interstitial fibroblast activation induced by transforming growth factor].
OBJECTIVE
To investigate the effects of leukemia inhibitory factor (LIF) on renal interstitial fibroblast activation following induction by transforming growth factor beta 1 (TGF-beta1).


METHODS
Normal rat interstitial fibroblast cells (NRK/49F) were treated with TGF-beta1 and TGF-beta1, combining with LIF respectively for different duration with different concentration. Changes in cell morphology and expression of alpha-SMA were evaluated with electronic microscope and Western blot respectively. The collagen I in the supernatant was detected with ABC-ELISA.


RESULTS
TGF-beta1 induced renal interstitial fibroblast activation, and this was accompanied by significant morphological transformations and secretion of collagen I. Co-culturing of cells with LIF blocked the morphological transformation. In addition, LIF inhibited TGF-beta1-induced expression of alpha-SMA mRNA and protein (P < 0.01), and decreased the levels of collagen I (P < 0.01) in a dose-dependent manner.


CONCLUSIONS
LIF suppresses TGF-beta1-induced activation and collagen I secretion of cultured renal interstitial fibroblasts.
Liu
Chang
C
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Wang
Li
L
Xie
Xi-Sheng
XS
Xie
Lin-Shen
LS
Li
Fei-Yan
FY
Wang
Feng-Ping
FP
Zhang
Lei
L
Zhang
He-Ping
HP
Fan
Jun-Ming
JM
D000199:Actins / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D005355:Fibrosis / Q000517:prevention & control; D007668:Kidney / Q000166:cytology* / Q000473:pathology; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051381:Rats; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D000199:Actins; D024042:Collagen Type I; D053605:Leukemia Inhibitory Factor; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat