Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of Inonotus obliquus by lignocellulose decomposition.
We reported that lignocellulose decomposition can be used to facilitate the production of bioactive polysaccharides from submerged culture of Inonotus obliquus. Exo-polysaccharide (EPS) production and antioxidant activity by Inonotus obliquus was enhanced by employing lignocellulose decomposition in a corn straw-containing submerged fermentation. A significant increase in the EPS production and hydroxyl radical scavenging activity from 1.09 ± 0.01 g/l and 72.3 ± 1.9% in a basal medium to 1.38 ± 0.02 g/l and 82.7 ± 0.5% in a corn straw-containing medium was obtained. A synchronized effect between lignocellulose decomposition and malondialdehyde presenting hydroxyl radical concentration in the fermentation broth was identified. The adding of thiourea, a hydroxyl radical-scavenging reagent, suppressed malondialdehyde generation and lowered the lignocellulose decomposition rate. Correspondingly, the EPS production and hydroxyl radical scavenging activity decreased to 1.26 g/l and 74%. The EPS obtained from the corn straw-containing medium also presented the strongest superoxide radical scavenging activity. The monosaccharide components of the EPS from the corn straw-containing medium are rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar proportions at 3.0, 3.0, 0.9, 46.6, 11.4, and 35.1%, respectively, which are largely different from the molar proportions of the EPS from the basal medium.
Chen
Hui
H
School of Science, Zhejiang Sci-Tech University, Hangzhou, China.
Yan
Mingchao
M
Zhu
Jinwei
J
Xu
Xiangqun
X
D000975:Antioxidants / Q000378:metabolism*; D001487:Basidiomycota / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000032:analysis; D005285:Fermentation; D017665:Hydroxyl Radical / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000096:biosynthesis* / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors; D003313:Zea mays / Q000378:metabolism*
D000975:Antioxidants; D003470:Culture Media; D009005:Monosaccharides; D011134:Polysaccharides; D013481:Superoxides; C036909:lignocellulose; D017665:Hydroxyl Radical; D008315:Malondialdehyde; C007916:hemicellulose; D008031:Lignin