Biochemical characterisation of Murray Valley encephalitis virus proteinase.
Murray Valley encephalitis virus (MVEV) is a member of the flavivirus group, a large family of single stranded RNA viruses, which cause serious disease in all regions of the world. Its genome encodes a large polyprotein which is processed by both host proteinases and a virally encoded serine proteinase, non-structural protein 3 (NS3). NS3, an essential viral enzyme, requires another virally encoded protein cofactor, NS2B, for proteolytic activity. The cloning, expression and biochemical characterisation of a stable MVEV NS2B-NS3 fusion protein is described.
Joy
Joma
J
Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*Star), Singapore, Singapore.
Mee
Ng Fui
NF
Kuan
Wee Liang
WL
Perlyn
Kwek Zekui
KZ
Wen
Then Siew
TS
Hill
Jeffrey
J
D018068:Encephalitis Virus, Murray Valley / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005416:Flavivirus / Q000235:genetics / Q000378:metabolism*; D020815:Polyproteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D057057:Serine Proteases; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020815:Polyproteins; D014764:Viral Proteins; D010450:Endopeptidases; D057057:Serine Proteases