title: Canine leishmaniasis. Immunophenotypic profile of leukocytes in different compartments of symptomatic, asymptomatic and treated dogs.
abstract: Canine visceral leishmaniasis (CanL) is an emerging disease, expanding in various parts of the world. The infection caused by Leishmania, an intracellular protozoan parasite, can show different clinical manifestations, from asymptomatic or subclinical to symptomatic dogs, in which a wide spectrum of clinical signs is evident. The fact that the parasite replicates in different organs raises the hypothesis that each organ may have a specific immune response. The local immune responses should be evaluated and taken into consideration when developing prophylactic tools. Therefore, phenotypic characterization of peripheral blood, lymph node and bone marrow lymphocyte populations and the expression of class II molecules of major histocompatibility complex (MHCII) were performed in asymptomatic and symptomatic dogs and in dogs that had been diagnosed and treated for leishmaniasis. Our findings showed that blood and bone marrow lymphocytes from symptomatic dogs were highly activated. In bone marrow of asymptomatic and treated dogs, a high frequency of MHCII(+) lymphocytes was observed, as well as MHCII(+) monocytes in the treated group. These results show increased expression of MHCII molecules giving evidence for antigenic presentation mainly by lymphocytes. The symptomatic and treated dogs showed an expansion of CD4(+) T cells subpopulations in lymph nodes, revealing an important contribution of these cells in controlling local parasite replication. This study also underlines the eventual importance of CD3(+)CD4(-)CD8(-) (double negative) and CD3(+)CD4(+)CD8(+) (double positive) T cell subsets in sensing and controlling latent infections and their possible function in the immune dynamics during CanL. The specific cellular immune responses raised in different compartments where the parasite replicates seem to have variable effects on local parasite control, highlighting the complexity of the cellular immune response developed by the dog infected by Leishmania infantum.
authors.affiliation: CIISA, Faculdade de Medicina Veterinária, Av. Universidade Técnica, 1300-477 Lisboa, Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Alexandre-Pires
authors.forename: Maria Teresa Villa
authors.initials: MT
authors.lastname: de Brito
authors.forename: Cármen
authors.initials: C
authors.lastname: Algueró
authors.forename: Catarina
authors.initials: C
authors.lastname: Martins
authors.forename: Olivia Roos
authors.initials: OR
authors.lastname: Rodrigues
authors.forename: Isabel Pereira
authors.initials: IP
authors.lastname: da Fonseca
authors.forename: Gabriela
authors.initials: G
authors.lastname: Santos-Gomes
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D056724:Immunity, Humoral; D016130:Immunophenotyping; D018314:Leishmania infantum / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000949:Histocompatibility Antigens Class II