title: Novel single nucleotide polymorphisms of the caprine PC1 gene and association with growth traits.
abstract: Proprotein convertase 1 (PC1) is an endopeptidase involved in proteolytic processing of peptide hormone precursors in granules of the regulated secretory pathway in endocrine cells. Mutations in the PC1 gene are thought to cause obesity, and multiple alternatively spliced transcript variants have been described for this gene. In this study, based on PCR-SSCP and DNA sequencing methods, polymorphisms of the PC1 gene were detected in 447 individuals from three breeds. Only the P1, P2, P3, P9, and P10 loci showed polymorphisms, and 12 SNPs in the PC1 gene were identified. Additionally, an association analysis between mutations in the 5'-UTR and four growth traits indicated that the polymorphisms were significantly associated with caprine body height (P < 0.05) and chest circumference (P < 0.01).
authors.affiliation: Institute of Cellular and Molecular Biology, Xuzhou Normal University, Jiangsu, 221116, Xuzhou, China.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Sun
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Xingtang
authors.initials: X
authors.lastname: Fang
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D005091:Exons / Q000235:genetics; D006041:Goats / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*; D007438:Introns / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D045664:Proprotein Convertase 1 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D045664:Proprotein Convertase 1