A microelectrochemical sensing system for the determination of Epstein-Barr virus antibodies.
An electrochemical method for the detection of Epstein-Barr virus (EBV) infections is described. The method relies on an immunoassay with electrochemical read-outs based on recombinant antigens. The antigens are immobilised on an Au electrode surface and used to complementarily bind antibodies from serum samples found during different stages of infection with EBV. Thiol chemistry under formation of self-assembled monolayers functions as a means to immobilise the antigens at the Au electrodes. A reporter system consisting of a secondary antibody labelled with alkaline phosphatase is used for electrochemical detection. The feasibility of the assay design is demonstrated and the assay performance is tested against the current gold standard in EBV detection. Close correlation is obtained for the results found for the developed electrochemical immunoassay and a standard line assay. Moreover, the electrochemical immunoassay is combined with a nanoporous electrode system allowing signal amplification by means of redox recycling. An amplification factor of 24 could be achieved.
Bandilla
Michael
M
FRIZ Biochem, Floriansbogen 2-4, 82061 Neuried, Germany.
Zimdars
Andreas
A
Neugebauer
Sebastian
S
Motz
Manfred
M
Schuhmann
Wolfgang
W
Hartwich
Gerhard
G
D000814:Aniline Compounds / Q000737:chemistry; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D058608:Nanopores; D009943:Organophosphorus Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry; D013379:Substrate Specificity; D013499:Surface Properties
D000814:Aniline Compounds; D000914:Antibodies, Viral; D000956:Antigens, Viral; D009943:Organophosphorus Compounds; D011994:Recombinant Proteins; C068798:4-aminophenylphosphate; D006046:Gold