title: FgTep1p is linked to the phosphatidylinositol-3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat.
abstract: Both mammalian tensin-like phosphatase 1 [TEP1; also known as phosphatase deleted on chromosome 10 (PTEN) or mutated in multiple advanced cancer 1 (MMAC1)] and Saccharomyces cerevisiae ScTep1p are involved in the phosphatidylinositol pathway. In this study, we identified the Fusarium graminearum locus FGSG_04982.3 (named FgTEP1) as the functional homologue of ScTEP1 in the sensitivity of S. cerevisiae cells to wortmannin, the phosphatidylinositol-3 kinase inhibitor. Deletion of FgTEP1 causes F. graminearum mycelial growth to become sensitive to lithium and reduces the production of conidia. Although conidia lacking FgTEP1 germinate normally, they show reduced germination efficiency in the presence of wortmannin. In addition, we showed that deletion of FgTEP1 reduces the virulence of F. graminearum on wheat. These results indicate that FgTep1p is linked to the phosphatidylinositol-3 kinase signalling pathway in this plant fungal pathogen.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Dajun
authors.initials: D
authors.lastname: Zhang
authors.forename: Feiyu
authors.initials: F
authors.lastname: Fan
authors.forename: Jingran
authors.initials: J
authors.lastname: Yang
authors.forename: Xuli
authors.initials: X
authors.lastname: Wang
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D008094:Lithium / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D014908:Triticum / Q000382:microbiology*; D014774:Virulence / Q000235:genetics / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D008094:Lithium; D019869:Phosphatidylinositol 3-Kinases