Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.
The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 A resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.23, b = 124.90, c = 164.80 A. The structure was solved by molecular replacement using the structure of T. brockii SADH (PDB entry 1ykf).
Protsko
Carla
C
Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Vieille
Claire
C
Laivenieks
Maris
M
Prasad
Lata
L
Sanders
David A R
DA
Delbaere
Louis T J
LT
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D007532:Isoleucine / Q000235:genetics; D009154:Mutation; D046968:Thermoanaerobacter / Q000201:enzymology*
D007532:Isoleucine; D000429:Alcohol Oxidoreductases; C025192:isopropanol dehydrogenase (NADP)