title: Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lymphocyte and cancer cell interaction by single-cell force spectroscopy.
abstract: The atomic force microscopy (AFM) is a powerful tool to analyze forces generated on cellular interactions on the single-cell level. This highly sensitive device can record changes in force in the pico-Newton range, which equals single molecule bonds. Here, we have used single-cell force spectroscopy by AFM to investigate the interaction between T cells and tumor cells that is induced by the bispecific antibody HEA125xOKT3 (specificity anti-EpCAMxCD3). We show that HEA125xOKT3 induces a specific increase in adhesion force between T cells and cancer cells. The adhesive force that is generated on cell-cell contact is dependent on the applied force on initial contact and the duration of this initial contact. In summary, HEA125xOKT3 has been found to mediate contact formation by distinct processes. It induces direct cell-cell interaction, which results in the activation of T-cell signaling, facilitates the formation of supramolecular activation clusters and ultimately of an immune synapse.
authors.affiliation: Institute of Physiology and Pathophysiology, Division of Cardiovascular Physiology, University of Heidelberg, Germany.
authors.forename: Sabrina C
authors.initials: SC
authors.lastname: Hoffmann
authors.forename: Guido H
authors.initials: GH
authors.lastname: Wabnitz
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Samstag
authors.forename: Gerhard
authors.initials: G
authors.lastname: Moldenhauer
authors.forename: Thomas
authors.initials: T
authors.lastname: Ludwig
mesh_terms: D018033:Antibodies, Bispecific / Q000737:chemistry / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002450:Cell Communication / Q000276:immunology*; D045744:Cell Line, Tumor; D002469:Cell Separation; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry; D006801:Humans; D054992:Immunological Synapses / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D018625:Microscopy, Atomic Force; D009369:Neoplasms / Q000276:immunology*; D013057:Spectrum Analysis; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
chemical_list: D018033:Antibodies, Bispecific; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule