Molecular comparison of Quebec and Newfoundland populations of the blackfly, Simulium vittatum, species complex.
Specific haplotypes at five positions in the COI and COII mitochondrial genes allowed a partial differentiation of Simulium vittatum Zetterstedt (Diptera: Simuliidae) populations from Quebec-Ontario and Newfoundland, respectively. This geographical signature was superimposed on about 40 other polymorphic sites such that sequence divergence alone did not enable a clear-cut distinction between the two populations. Together with the sporadic occurrence of haplotypes intermediate to the Newfoundland and Quebec-Ontario consensus, this suggested that one peculiar sequence among many found in populations from the North American landmass predominates in Newfoundland as a result of a founder effect. The internal transcribed spacer (ITS1) sequence from the nuclear rDNA transcription unit was no more able to resolve populations along geographical lines than the COI/COII criteria.
Gaudreau
C
C
Département de Chimie-biologie, Université du Québec à Trois-Rivières, Trois-Rivières, Quebec G9A 5H7, Canada.
LaRue
B
B
Charpentier
G
G
D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D008969:Molecular Sequence Data; D009523:Newfoundland and Labrador; D011792:Quebec; D012843:Simuliidae / Q000201:enzymology / Q000235:genetics*; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV