Quantification of urinary 8-iso-prostaglandin F2alpha using liquid chromatography-tandem mass spectrometry during cardiac valve surgery.
Oxidative stress is an unavoidable event during many complex surgical procedures. 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) is a reliable biomarker for the evaluation of oxidative stress in vivo. The aim of this study is to develop simple and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for the detection of urinary 8-iso-PGF(2alpha) in samples collected from patients who received a cardiopulmonary bypass (CPB) during cardiac valve surgery. Urine samples of 14 patients with cardiac valve diseases were collected before, during, and after CPB. The level of 8-iso-PGF(2alpha) was detected via selected-reaction monitoring triple quadrupole MS/MS and the result was compared with 12 healthy volunteers. The method's detection limit (3S/N) was 0.25 pg for 8-iso-PGF(2alpha), with a linear working range of 0.25-20 ng/ml. For patients with cardiac valve disease, the 8-iso-PGF(2alpha) levels before the bypasses were the same as those of healthy individuals (P>0.05) and the 8-iso-PGF(2alpha) levels during and after CPB were significantly higher than those before the bypasses (P<0.05). In conclusion, we present a simple and specific protocol for LC-MS/MS quantification of urinary 8-iso-PGF(2alpha) collected during CPB. Using this technique, it would be feasible to assess the levels of oxidative stress during cardiac surgery and thereby helpful for the management of oxidative injury.
Teng
Yun-Hui
YH
Department of Anesthesiology, Chang Gung Memorial Hospital, Chang Gung University School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
Wang
Chih-Wei
CW
Liao
Yun-Tsan
YT
Yang
Min-Wen
MW
Liu
Tsung-Yun
TY
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006351:Heart Valves / Q000601:surgery*; D006801:Humans; D016014:Linear Models; D008297:Male; D018384:Oxidative Stress; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost