title: KIAA1718 is a histone demethylase that erases repressive histone methyl marks.
abstract: The methylation states of histone lysine residues are regarded as significant epigenetic marks governing transcriptional regulation. A number of histone demethylases containing a jumonji C (JmjC) domain have been recognized; however, their properties remain to be investigated. Here, we show that KIAA1718, a PHF2/PHF8 subfamily member, possesses histone demethylase activity specific for H3K9 and H3K27, transcriptionally repressive histone marks. Biochemical purification of the KIAA1718 interactants reveals that KIAA1718 forms complexes with several factors including KAP1, a transcriptional co-activator. Consistent with these findings, KIAA1718 shows a transcriptional activation function in the chromatin context. Thus, our study identifies KIAA1718 as a histone demethylase for repressive methyl marks and shows that it is involved in transcriptional activation.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yokoyama
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Okuno
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Chikanishi
authors.forename: Waka
authors.initials: W
authors.lastname: Hashiba
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sekine
authors.forename: Ryoji
authors.initials: R
authors.lastname: Fujiki
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D056466:Histone Demethylases / Q000737:chemistry / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D051379:Mice
chemical_list: D006657:Histones; D056466:Histone Demethylases; C555910:KIAA1718 protein, mouse; D008239:Lysine