In vitro methods of evaluating antithrombotics and thrombolytics.
Platelets play a crucial role in primary hemostasis by forming hemostatic plugs at sites of vascular injury. There is abundant evidence that platelets also play a pivotal role in the pathogenesis of arterial thrombotic disorders, including unstable angina (UA), myocardial infarction (MI), and stroke. The underlying pathophysiological mechanism of these processes has been recognized as the disruption or erosion of a vulnerable atherosclerotic plaque, leading to local platelet adhesion and subsequent formation of partially or completely occlusive platelet thrombi. A variety of methods have been used to assess platelet aggregation, blood coagulation, and the ex vivo and/or in vitro efficacy of platelet antagonists, anticoagulants, and thrombolytics.
Mousa
Shaker A
SA
Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY, USA.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002850:Chromatography, Gel; D003094:Collagen / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D007834:Lasers; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D039341:Receptors, Fibrinogen / Q000378:metabolism; D012212:Rheology; D013916:Thrombelastography / Q000187:drug effects; D013927:Thrombosis / Q000188:drug therapy / Q000503:physiopathology; D014783:Viscosity
D005343:Fibrinolytic Agents; D039341:Receptors, Fibrinogen; D003094:Collagen