title: Preparation and characterization of iron oxide-silica composite particles using mesoporous SBA-15 silica as template and their internalization into mesenchymal stem cell and human bone cell lines.
abstract: A new procedure for preparing iron oxide-silica nanocomposite particles using SBA-15 mesoporous silica as a template is described. These composite materials retained the 2-D hexagonal structure of the SBA-15 template. Transmission electron micrograms of the particles depicted the formation of iron oxide nanocrystals inside the mesochannels of SBA-15 silica framework. Powder x-ray diffraction showed that the iron oxide core of the composite particles consists of a mixture of maghemite (gamma-Fe(2)O(3)) and heamatite (alpha-Fe(2)O(3)), which is the predominant component. Superconducting quantum interference device (SQUID) magnetometry studies showed that these iron oxide-silica composite materials exhibit superparamagnetic properties. On increasing the iron oxide content, the composite particles exhibited a stronger response to magnetic fields but a less homogeneous core, with some large iron oxide particles which were thought to be formed outside the mesochannels of the SBA-15 template. Internalization of these particles into human cell lines (mesenchymal stem cells and human bone cells), which indicates their potential in medicine and biotechnology, is also discussed.
authors.affiliation: Institute of Science and Technology in Medicine, Keele University, Stoke-on-Trent, ST4 7QB, UK. h.yiu@liv.ac.uk
authors.forename: Humphrey H P
authors.initials: HH
authors.lastname: Yiu
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Maple
authors.forename: Martin R
authors.initials: MR
authors.lastname: Lees
authors.forename: Iryna
authors.initials: I
authors.lastname: Palona
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: El Haj
authors.forename: Jon
authors.initials: J
authors.lastname: Dobson
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005290:Ferric Compounds / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry* / Q000493:pharmacokinetics*; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C509969:SBA-15; C000499:ferric oxide; D012822:Silicon Dioxide