title: Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina.
abstract: The polysaccharide (PAP) from Potentilla anserina was evaluated for modulating effects by using mouse peritoneal macrophage and the immunosuppressed-model cyclophosphamide-induced. Phagocytotic and mononuclear phagocytic system function assays showed that PAP stimulated the phagocytosis of phagocyte. Splenocyte proliferation assay showed that PAP acted the effect combining Con A or LPS in splenocyte proliferation. The parameters detected showed that PAP increased thymus and spleen indices, the levels of LDH and ACP in the spleen, and IL-10 and IFN-γ in serum in immunosuppressed mice. The results suggest that PAP is involved in immunomodulatory effects leading to the exploration for PAP as a potential immunostimulant.
authors.affiliation: Lanzhou Institute of Animal Sciences and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, No. 335 Jiangouyan, Xiaoxihu, Lanzhou, Gansu 730050, PR China.
authors.forename: Jiong-Ran
authors.initials: JR
authors.lastname: Chen
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Ting-Jun
authors.initials: TJ
authors.lastname: Hu
authors.forename: Zuo-Ting
authors.initials: ZT
authors.lastname: Yan
authors.forename: Ting-Xian
authors.initials: TX
authors.lastname: Niu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Dong-An
authors.initials: DA
authors.lastname: Cui
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D056747:Immunomodulation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D031987:Potentilla / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects
chemical_list: D011134:Polysaccharides; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase