title: Nitrifying bacterial growth inhibition in the presence of algae and cyanobacteria.
abstract: Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global nitrogen cycling. A continuous flow autotrophic bioreactor was initially designed for nitrifying bacterial growth only. In the presence of cyanobacteria and algae, we monitored both the microbial activity by measuring specific oxygen production rate (SOPR) for microalgae and cyanobacteria and specific oxygen uptake rate (SOUR) for nitrifying bacteria. The growth of cyanobacteria and algae inhibited the maximum nitrification rate by a factor of 4 although the ammonium nitrogen fed to the reactor was almost completely removed. Terminal restriction fragment length polymorphism (T-RFLP) analysis indicated that the community structures of nitrifying bacteria remained unchanged, containing the dominant Nitrosospira, Nitrospira, and Nitrobacter species. PCR amplification coupled with cloning and sequencing analysis resulted in identifying Chlorella emersonii and an uncultured cyanobacterium as the dominant species in the autotrophic bioreactor. Notwithstanding their fast growth rate and their toxicity to nitrifiers, microalgae and cyanobacteria were more easily lost in effluent than nitrifying bacteria because of their poor settling characteristics. The microorganisms were able to grow together in the bioreactor with constant individual biomass fractions because of the uncoupled solids retention times for algae/cyanobacteria and nitrifiers. The results indicate that compared to conventional wastewater treatment systems, longer solids retention times (e.g., by a factor of 4) should be considered in phototrophic bioreactors for complete nitrification and nitrogen removal.
authors.affiliation: Department of Civil and Environmental Engineering, University of Missouri, Columbia, Missouri, USA.
authors.forename: Okkyoung
authors.initials: O
authors.lastname: Choi
authors.forename: Atreyee
authors.initials: A
authors.lastname: Das
authors.forename: Chang-Ping
authors.initials: CP
authors.lastname: Yu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000898:Antibiosis; D019149:Bioreactors / Q000382:microbiology*; D000460:Chlorophyta / Q000254:growth & development*; D016172:DNA Fingerprinting; D058465:Nitrification; D043369:Nitrosomonadaceae / Q000254:growth & development* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D014871:Water Microbiology; D018508:Water Purification
chemical_list: D010100:Oxygen