title: Ultrasonication extraction and gel permeation chromatography clean-up for the determination of polycyclic aromatic hydrocarbons in edible oil by an isotope dilution gas chromatography–mass spectrometry.
abstract: An analytical method for the determination of US EPA priority pollutant 16 polycyclic aromatic hydrocarbons (PAHs) in edible oil was developed by an isotope dilution gas chromatography-mass spectrometry (GC-MS). Extraction was performed with ultrasonication mode using acetonitrile as solvent, and subsequent clean-up was applied using narrow gel permeation chromatographic column. Three deuterated PAHs surrogate standards were used as internal standards for quantification and analytical quality control. The limits of quantification (LOQs) were globally below 0.5 ng/g, the recoveries were in the range of 81-96%, and the relative standard deviations (RSDs) were lower than 20%. Further trueness assessment of the method was also verified through participation in international cocoa butter proficiency test (T0638) organised by the FAPAS with excellent results in 2008. The results obtained with the described method were satisfying (z ≤ 2). The method has been applied to determine PAH in real edible oil samples.
authors.affiliation: Shandong Exit & Entry Inspection and Quarantine Bureau, State Key Laboratory of Food Safety Testing, Qingdao, Shandong, China. whywrs@yahoo.com.cn
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Cui
authors.initials: C
authors.lastname: Guo
mesh_terms: D000097:Acetonitriles; D002850:Chromatography, Gel / Q000379:methods*; D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D005224:Fats, Unsaturated / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007554:Isotopes; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D014465:Ultrasonics / Q000379:methods
chemical_list: D000097:Acetonitriles; D004042:Dietary Fats, Unsaturated; D005224:Fats, Unsaturated; D007554:Isotopes; D011084:Polycyclic Aromatic Hydrocarbons; C032159:acetonitrile