title: Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis.
abstract: We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.
authors.affiliation: Johns Hopkins University Department of Emergency Medicine, 5801 Smith Avenue, Suite 3220, Davis Building, Baltimore, MD 21209, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: Won
authors.forename: Richard
authors.initials: R
authors.lastname: Rothman
authors.forename: Padmini
authors.initials: P
authors.lastname: Ramachandran
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Hsieh
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Kecojevic
authors.forename: Karen C
authors.initials: KC
authors.lastname: Carroll
authors.forename: Deborah
authors.initials: D
authors.lastname: Aird
authors.forename: Charlotte
authors.initials: C
authors.lastname: Gaydos
authors.forename: Samuel
authors.initials: S
authors.lastname: Yang
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D044366:Transition Temperature
chemical_list: D004269:DNA, Bacterial