Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids.
Assays based on real-time PCR (TaqMan) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd.
Monger
Wendy
W
Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK. wendy.monger@fera.gsi.gov.uk
Tomlinson
Jenny
J
Booonham
Neil
N
Marn
Mojca Virscek
MV
Plesko
Irena Mavric
IM
Molinero-Demilly
Valérie
V
Tassus
Xavier
X
Meekes
Ellis
E
Toonen
Marcel
M
Papayiannis
Lambros
L
Perez-Egusquiza
Zoila
Z
Mehle
Natasa
N
Jansen
Claudia
C
Nielsen
Steen Lykke
SL
D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D019657:Solanaceae / Q000821:virology*; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*
D012367:RNA, Viral