Staphylococcus aureus promoter-lux reporters for drug discovery.
We describe a collection of antibiotic-activated Staphylococcus aureus promoter-lux reporter strains that can be used to discriminate among antibiotic classes on the basis of their light production response profile. We screened over 400 culture supernatants from previously uncharacterized actinomycetes from soil for the production of aminocoumarin-type compounds and DNA-damaging agents. Novobiocin production was determined in three isolates of Streptomyces, and streptonigrin, a DNA-damaging agent, together with several other bioactive compounds (oxopropaline D and G), was identified from a novel Kitasatospora isolate. This array provides an effective and specific whole-cell approach to search for classes of antimicrobial compounds in unfractionated culture broths.
Mesak
Lili R
LR
Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.
Qi
Shuhua
S
Villanueva
Ivan
I
Miao
Vivian
V
Davies
Julian
J
D039903:Actinobacteria / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D055808:Drug Discovery / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D013302:Streptomyces / Q000378:metabolism
D000900:Anti-Bacterial Agents; D008156:Luciferases