Oral vaccination with the porcine rotavirus VP4 outer capsid protein expressed by Lactococcus lactis induces specific antibody production.
The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An approximately 27 kDa VP4 protein was confirmed by SDS-PAGE , Western blot and immunostaining analysis. BALB/c mice were immunized orally with VP4-expression recombinant L. lactis and cellular, mucosal and systemic humoral immune responses were examined. Specific anti-VP4 secretory IgA and IgG were found in feces, ophthalmic and vaginal washes and in serum. The induced antibodies demonstrated neutralizing effects on porcine rotavirus infection on MA104 cells. Our findings suggest that oral immunization with VP4-expressing L. lactis induced both specific local and systemic humoral and cellular immune responses in mice.
Li
Yi-jing
YJ
College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. yijingli@yahoo.com
Ma
Guang-peng
GP
Li
Gui-wei
GW
Qiao
Xin-yuan
XY
Ge
Jun-wei
JW
Tang
Li-jie
LJ
Liu
Min
M
Liu
Li-wei
LW
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007118:Immunoassay; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D013294:Lactococcus lactis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control; D022243:Rotavirus Vaccines / Q000276:immunology*; D034421:Sus scrofa / Q000821:virology*; D014611:Vaccination
D036022:Capsid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; C060339:VP4 protein, Rotavirus