title: Quantitative 1H-NMR spectrometry method for quality control of Aloe vera products.
abstract: An 1H-NMR spectrometry method was developed and validated to quantify acetylated polysaccharides, glucose, malic acid, lactic acid, and acetic acid in Aloe vera products. Nicotinamide was selected as an internal standard (IS) in this quantification method. The quantity of marker compounds was calculated by the relative ratio of the integrals of each compound to the IS. The method validation included specificity, linearity, accuracy, robustness, reproducibility, LOD, and LOQ on two samples--A. vera inner leaf standard and decolorized aloe whole leaf Standard from the International Aloe Science Council. Eighteen A. vera commercial products were analyzed with this NMR spectrometry method.
authors.affiliation: Unigen, Inc., 2660 Willamette Dr NE, Lacey, WA 98516, USA. pingj@unigen.net
authors.forename: Ping
authors.initials: P
authors.lastname: Jiao
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.forename: Gabriele
authors.initials: G
authors.lastname: Randel
authors.forename: Bernd
authors.initials: B
authors.lastname: Diehl
authors.forename: Stacy
authors.initials: S
authors.lastname: Weaver
authors.forename: Gregory
authors.initials: G
authors.lastname: Milligan
mesh_terms: D000504:Aloe / Q000737:chemistry*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011786:Quality Control; D012680:Sensitivity and Specificity