title: [The C-terminal domain of the Vibrio fischeri transcription activator LuxR is not essential for degradation by Lon protease].
abstract: The Vibrio fischer luxICDABEG genes are activated by autoinducer N-(3-oxohexanoyl)homoserine lactone and the LuxR protein. The LuxR contains 250 aa and consists of two domains. The C-domain, that extends from around residue 162 to the C-terminus, is thought to bind lux regulatory DNA and activate transcription of the luxICDABEG genes. The N-terminal domain, which binds the autoinducer, consists of about 70% residues of LuxR. In E. coli C-terminal domain can activate the lux genes in the absence of autoinducer. Previously it was shown that the ATP-dependent Lon protease of E. coli takes part in the negative regulation of the transcription of the V. fischeri lux operon and that LuxR is a target of Lon protease. Comparative analysis of effects of Lon protease on the V. fischeri luxICDABEG genes expression was made. Special constructed hybrid plasmids which permit the regulation of luxR, luxR 5'-deletion mutation were used and luICDABEG genes were activated independently and quantitatively. We show that the full length LuxR, but not C-terminal domain is a target protein for Lon protease. The transcription activity by full length LuxR protein isobserved when its intracellular concentration is about two order lower than that of its C-terminal domain.
authors.forename: O E
authors.initials: OE
authors.lastname: Mel'kina
authors.forename: I V
authors.initials: IV
authors.lastname: Manukhov
authors.forename: G B
authors.initials: GB
authors.lastname: Zavil'gel'skiĭ
mesh_terms: D048908:Aliivibrio fischeri / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000502:physiology; D009876:Operon / Q000502:physiology; D049070:Protease La / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; D049070:Protease La