title: Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.
abstract: Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs ("TiPS") retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages.
authors.affiliation: Department of Research and Development, Cellular Dynamics International, Inc., Madison, Wisconsin, United States of America.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Brown
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Rondon
authors.forename: Deepika
authors.initials: D
authors.lastname: Rajesh
authors.forename: Amanda
authors.initials: A
authors.lastname: Mack
authors.forename: Rachel
authors.initials: R
authors.lastname: Lewis
authors.forename: Xuezhu
authors.initials: X
authors.lastname: Feng
authors.forename: Laura Jo
authors.initials: LJ
authors.lastname: Zitur
authors.forename: Randall D
authors.initials: RD
authors.lastname: Learish
authors.forename: Emile F
authors.initials: EF
authors.lastname: Nuwaysir
mesh_terms: D000328:Adult; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D013601:T-Lymphocytes / Q000166:cytology*