A new source of cytoplasmic male sterility found in wild beet and its relationship to other CMS types.
We found a number of male-sterile plants in a wild beet (Beta vulgaris L. subsp. maritima) accession line, FR4-31. The inheritance study of the male sterility indicated the trait to be of the cytoplasmic type. The mitochondrial genome of FR4-31 proved to lack the male-sterility-associated genes preSatp6 and orf129, which are characteristic of the Owen CMS and I-12CMS(3) cytoplasms of beets, respectively. Instead, the truncated cox2 gene involved in G CMS originating from wild beets was present in the FR4-31 mitochondrial genome. In Southern hybridization using four mitochondrial gene probes, the FR4-31 cytoplasm showed patterns similar to those typical of the G cytoplasm. It is thus likely that the FR4-31 cytoplasm has a different CMS mechanism from both Owen CMS and I-12CMS(3), and that the FR4-31 and G cytoplasms resemble each other closely. A restriction map of the FR4-31 mitochondrial DNA was generated and aligned with those published for the Owen and normal fertile cytoplasms. The FR4-31 mitochondrial genome was revealed to differ extensively in arrangement from the Owen and normal genomes, and the male-sterile Owen and FR4-31 genomes seem to be derived independently from an ancestral genome.
Kawanishi
Yuki
Y
Laboratory of Genetic Engineering, Research Faculty of Agriculture, Hokkaido University, N-9, W-9, Kita-ku, Sapporo 060-8589, Japan.
Shinada
Hiroshi
H
Matsunaga
Muneyuki
M
Masaki
Yusuke
Y
Mikami
Tetsuo
T
Kubo
Tomohiko
T
D027461:Beta vulgaris / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D015153:Blotting, Western; D003593:Cytoplasm / Q000235:genetics; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D023061:Gene Order; D054629:Genome, Mitochondrial / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D004272:DNA, Mitochondrial; D018744:DNA, Plant; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D003576:Electron Transport Complex IV