Development of fluorescent array based on sol-gel/chitosan encapsulated acetylcholinesterase and pH sensitive oxazol-5-one derivative.
A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane/chitosan matrix via a microarrayer. The constructed tetraethoxysilane/chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 × 10(-8) M, with a detection limit of 0.27 × 10(-8) M.
Ozturk
Gulsiye
G
Faculty of Arts and Sciences, Department of Chemistry, University of Dokuz Eylul, Izmir, Turkey. gulsiye.ozturk@deu.edu.tr
Feller
Karl-Heinz
KH
Dornbusch
Kay
K
Timur
Suna
S
Alp
Serap
S
Ergun
Yavuz
Y
D000109:Acetylcholine / Q000032:analysis; D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010080:Oxazoles / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D010080:Oxazoles; D048271:Chitosan; D000110:Acetylcholinesterase; D000109:Acetylcholine