title: Expression of yeast cyclophilin A (Cpr1) provides improved stress tolerance in Escherichia coli.
abstract: Cyclophilins contain the conserved activity of cis-trans peptidyl-prolyl isomerase that is implicated in protein folding and function as molecular chaperones. The yeast cyclophilin A gene (cpr1) was subcloned to the prokaryotic expression vector pKM260. It was found that the expression of Cpr1 drastically increased the cell viability of E. coli BL21 in the presence of abiotic stress conditions, such as cadmium, copper, hydrogen peroxide, heat, and SDS. Thus, this study illustrates the importance of Cpr1 as a molecular chaperone that improved cellular stress responses when E. coli cells were exposed to adverse conditions, and it also shows the possibility of increasing the stability of E. coli strains utilized for the production of recombinant proteins.
authors.affiliation: Department of Biology, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Il-Sup
authors.initials: IS
authors.lastname: Kim
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Shin
authors.forename: Young-Saeng
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyun-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Kyung Moc
authors.initials: KM
authors.lastname: Park
authors.forename: Ho-Sung
authors.initials: HS
authors.lastname: Yoon
authors.forename: Ingnyol
authors.initials: I
authors.lastname: Jin
mesh_terms: D021984:Cyclophilin A / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D021984:Cyclophilin A