[An in vitro study of anti-human cytomegalovirus effect of Forsythia suspensa and its main active ingredient quercetin].
OBJECTIVE
To study the in vitro anti-human cytomegalovirus effect and the cytotoxicity of Forsythia suspensa and its main active ingredient quercetin.


METHODS
The 0% toxic dose (TD0), minimum effective concentration (MEC) and therapeutic index (TI) of anti-human cytomegalovirus activity by F. suspensa and quercetin were detected with the cytopathic assay and MTT method. Ganciclovir was used as the control drug for comparison.


RESULTS
The TD0 of ganciclovir, F. suspensa and quercetin were 10, 30, 30 mg L(-1), the MEC were 10, 30, 0.3 mg x L(-1), TI were 1, 1 and 100, respectively.


CONCLUSIONS
The anti-human cytomegalovirus effect of quercetin is much higher than ganciclovir and F. suspensa, and the cytotoxicity is equivalent to F. suspensa but lower than ganciclovir. Therefore, quercetin has the potential advantages of anti-human cytomegalovirus effect.
Zhang
Dandan
D
Department of Obstetrics and Gynecology, Tongli Hospital Affiliated with Tongfi Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. beautyzdandan@yahoo.com.cn
Fang
Jianguo
J
Chen
Juanjuan
J
Wan
Jin
J
Zhang
Yawei
Y
Yin
Zongzhi
Z
Wang
Nan
N
Chen
Suhua
S
D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000633:toxicity; D031657:Forsythia / Q000737:chemistry*; D006801:Humans; D011794:Quercetin / Q000494:pharmacology* / Q000633:toxicity
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D011794:Quercetin