title: Primo-vessels and primo-nodes in rat brain, spine and sciatic nerve.
abstract: We report a method using Trypan blue staining to detect primo-vessels in the nervous system on internal organs or in the skin of rat. We applied this technique to visualize the primo-vessels and primo-nodes in the brain, spinal cord and sciatic nerve of a rat. Primo-vessels and primo-nodes were preferentially stained at nerves, blood vessels, or fascia-like membranes and turned blue after the spread and washing of Trypan blue. The physiological role of the primo-vessels within the nervous system is an important question warranting further investigation.
authors.affiliation: Biomedical Physics Laboratory for Korean Medicine, Department of Physics and Astronomy, Seoul National University, Korea. donboscolee@gmail.com
authors.forename: Byung-Cheon
authors.initials: BC
authors.lastname: Lee
authors.forename: Ki-Hoon
authors.initials: KH
authors.lastname: Eom
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D001808:Blood Vessels / Q000737:chemistry*; D001921:Brain / Q000098:blood supply*; D001923:Brain Chemistry; D008297:Male; D016740:Meridians; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000098:blood supply* / Q000737:chemistry; D013116:Spinal Cord / Q000098:blood supply* / Q000737:chemistry; D013194:Staining and Labeling; D014343:Trypan Blue / Q000032:analysis
chemical_list: D014343:Trypan Blue