title: Identification of putative biomarkers for toluene-degrading Burkholderia and pseudomonas by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting.
abstract: The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.
authors.affiliation: Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Erica M
authors.initials: EM
authors.lastname: Hartmann
authors.forename: David R
authors.initials: DR
authors.lastname: Colquhoun
authors.forename: Rolf U
authors.initials: RU
authors.lastname: Halden
mesh_terms: D001673:Biodegradation, Environmental; D015415:Biomarkers / Q000032:analysis; D019117:Burkholderia / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010449:Peptide Mapping; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014050:Toluene / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014050:Toluene