Adhesive properties, extracellular protein production, and metabolism in the Lactobacillus rhamnosus GG strain when grown in the presence of mucin.
This paper examines the probiotic bacterium Lactobacillus rhamnosus GG, and how it reacts to the presence of mucin in its extracellular milieu. Parameters studied included cell clustering, adhesion to mucin, extracellular protein production, and formation of final metabolites. L. rhamnosus GG was found to grow efficiently in the presence of glucose, N-acetylglucosamine, or mucin (partially purified or purified) as sole carbon sources. However, it was unable to grow using other mucin constituents, such as fucose or glucuronic acid. Mucin induced noticeable changes in all the parameters studied when compared with growth using glucose, including in the formation of cell clusters, which were easily disorganized with trypsin. Mucin increased adhesion of the bacterium, and modulated the production of extracellular proteins. SDS-PAGE revealed that mucin was not degraded during L. rhamnosus GG growth, suggesting that this bacterium is able to partially use the glucidic moiety of glycoprotein. This study goes some way towards developing an understanding of the metabolic and physiological changes that L. rhamnosus GG undergoes within the human gastrointestinal tract.
Sanchez
Borja
B
Universite de Bordeaux, UMR 5248 CNRS, UBX1-ENITAB, ENITAB, 1 cours du General de Gaulle, 33175 Gradignan Cedex, France. borja@ipla.csic.es
Saad
Naima
N
Schmitter
Jean-Marie
JM
Bressollier
Philippe
P
Urdaci
Maria C
MC
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry / Q000254:growth & development / Q000502:physiology*; D008954:Models, Biological; D009077:Mucins / Q000378:metabolism*; D013552:Swine
D001426:Bacterial Proteins; D009077:Mucins