Sumoylation of Drosophila transcription factor STAT92E.
STAT92E is an essential transcription factor in Drosophila melanogaster for the development of several organs and the immune system. The JAK/STAT pathway employs different evolutionary conserved regulatory mechanisms to control biological processes. Numerous transcription factors in both mammals and invertebrates have been shown to be either activated or inhibited by a covalent modification with a small ubiquitin-like modifier (Sumo). Here, we show that Drosophila STAT92E is modified by Sumo at a single lysine residue 187 in S2 cells. Mutation of Lys187 increases the transcriptional activity of STAT92E, thus suggesting that sumoylation of STAT92E has a repressive role in the regulation of the JAK/STAT pathway in Drosophila melanogaster.
Grönholm
Juha
J
Institute of Medical Technology, University of Tampere, Tampere, Finland.
Ungureanu
Daniela
D
Vanhatupa
Sari
S
Rämet
Mika
M
Silvennoinen
Olli
O
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics; D038081:Organogenesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D058207:Sumoylation / Q000235:genetics
D029721:Drosophila Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D050791:STAT Transcription Factors; D025842:SUMO-1 Protein; C105675:Stat92E protein, Drosophila