Antimicrobial activity of terpenoids from Copaifera langsdorffii Desf. against cariogenic bacteria.
In the present work, the anticariogenic activities of nine labdane type-diterpenes and four sesquiterpenes were investigated. Among these metabolites, (-)-copalic acid (CA) was the most active compound displaying MIC values very promising (ranging from 2.0 to 6.0 µg/mL) against the main microorganisms responsible for dental caries: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis and Lactobacillus casei. Time kill assays performed with CA against the primary causative agent (S. mutans) revealed that, in the first 12 h, this compound only inhibits the growth of the inoculum (bacteriostatic effect). However, its bactericidal effect is clearly noted thereafter (between 12 and 24 h). Also, CA did not show a synergistic effect when combined with the anticariogenic gold standard (chlorhexidine, CHD) in the checkerboard assays against S. mutans. In conclusion, the results points out CA as an important metabolite in the search for new effective anticariogenic agents.
Souza
Ariana B
AB
Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil.
Martins
Carlos H G
CH
Souza
Maria G M
MG
Furtado
Niege A J C
NA
Heleno
Vladimir C G
VC
de Sousa
João P B
JP
Rocha
Erilda M P
EM
Bastos
Jairo K
JK
Cunha
Wilson R
WR
Veneziani
Rodrigo C S
RC
Ambrósio
Sérgio R
SR
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology; D004224:Diterpenes / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D012717:Sesquiterpenes / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010936:Plant Extracts; D012717:Sesquiterpenes; C556511:copalic acid