beta-Glucan oligosaccharide enhances CD8(+) T cells immune response induced by a DNA vaccine encoding hepatitis B virus core antigen.
DNA vaccination can induce specific CD8(+) T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose (beta-glu6), an analogue of Lentinan basic unit, on specific CD8(+) T cell response induced by a DNA vaccine encoding HBcAg (pB144) in mice. We found that beta-glu6 promoted the recruitment and maturation of dendritic cells, enhanced the activation of CD8(+) and CD4(+) T cells and increased the number of specific CD8(+)/IFN-gamma(+) T cells in lymphoid and nonlymphoid tissues in mice immunized by pB144. Immunization with pB144 and beta-glu6 increased the anti-HBc IgG and IgG2a antibody titer. These results demonstrate that beta-glu6 can enhance the virus-specific CTL and Th1 responses induced by DNA vaccine, suggesting beta-glu6 as a candidate adjuvant in DNA vaccination.
Wang
Jing
J
Key laboratory of Medical Molecular Virology of Ministries of Education and Health, Institute of Medical Microbiology and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Dong
Shengfu
S
Liu
Chunhong
C
Wang
Wei
W
Sun
Shuhui
S
Gu
Jianxin
J
Wang
Yuan
Y
Boraschi
Diana
D
Qu
Di
D
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D039521:CD11c Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019556:COS Cells; D002454:Cell Differentiation / Q000276:immunology; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D007109:Immunity / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000276:immunology*; D010957:Plasmids / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D047071:beta-Glucans / Q000276:immunology*
D039521:CD11c Antigen; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D017325:Hepatitis B Vaccines; D009844:Oligosaccharides; D019444:Vaccines, DNA; D047071:beta-Glucans; D007371:Interferon-gamma