Gentamicin-loaded wound dressing with polyvinyl alcohol/dextran hydrogel: gel characterization and in vivo healing evaluation.
To develop a gentamicin-loaded wound dressing, cross-linked hydrogel films were prepared with polyvinyl alcohol (PVA) and dextran using the freezing-thawing method. Their gel properties such as gel fraction, swelling, water vapor transmission test, morphology, tensile strength, and thermal property were investigated. In vitro protein adsorption test, in vivo wound healing test, and histopathology were performed. Dextran decreased the gel fraction, maximum strength, and thermal stability of hydrogels. However, it increased the swelling ability, water vapor transmission rate, elasticity, porosity, and protein adsorption. The drug gave a little positive effect on the gel properties of hydrogels. The gentamicin-loaded wound dressing composed of 2.5% PVA, 1.13% dextran, and 0.1% drug was more swellable, flexible, and elastic than that with only PVA because of its cross-linking interaction with PVA. In particular, it could provide an adequate level of moisture and build up the exudates on the wound area. From the in vivo wound healing and histological results, this gentamicin-loaded wound dressing enhanced the healing effect more compared to conventional product because of the potential healing effect of gentamicin. Thus, this gentamicin-loaded wound dressing would be used as a potential wound dressing with excellent forming and improved healing effect in wound care.
Hwang
Ma-Ro
MR
College of Pharmacy, Yeungnam University, 214-1, Dae-Dong, Gyongsan, 712-749, South Korea.
Kim
Jong Oh
JO
Lee
Jeong Hoon
JH
Kim
Yong Il
YI
Kim
Jeong Hoon
JH
Chang
Sun Woo
SW
Jin
Sung Gju
SG
Kim
Jung Ae
JA
Lyoo
Won Seok
WS
Han
Sung Soo
SS
Ku
Sae Kwang
SK
Yong
Chul Soon
CS
Choi
Han-Gon
HG
D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001458:Bandages; D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis*; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology
D000900:Anti-Bacterial Agents; D003911:Dextrans; D004337:Drug Carriers; D005839:Gentamicins; D020100:Hydrogels; D011142:Polyvinyl Alcohol