Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis.
We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.
Won
Helen
H
Johns Hopkins University Department of Emergency Medicine, 5801 Smith Avenue, Suite 3220, Davis Building, Baltimore, MD 21209, USA.
Rothman
Richard
R
Ramachandran
Padmini
P
Hsieh
Yu-Hsiang
YH
Kecojevic
Aleksandar
A
Carroll
Karen C
KC
Aird
Deborah
D
Gaydos
Charlotte
C
Yang
Samuel
S
D016470:Bacteremia / Q000175:diagnosis*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors; D044366:Transition Temperature
D004269:DNA, Bacterial