title: Seroepidemiology of porcine enteric sapovirus in pig farms in Venezuela.
abstract: Porcine enteric sapovirus (PES) has been shown to cause diarrhea under experimental conditions in gnotobiotic piglets. However, the role of PES as enteric pathogens in porcine farms remains unclear. To further understand the PES-host interactions under field conditions, a serological survey was carried out. To this end the capsid gene of a PES isolate was cloned in the baculovirus expression system and an ELISA was developed based on virus-like particles from the baculovirus-expressed PES capsid protein. A total of 85 serum samples collected from pigs ranging from 8 weeks to over 54 weeks of age were analyzed. An overall seroprevalence to PESs of 62% was found, with significant differences (p<0.05) found between ages. Pigs younger than 10 weeks old and older than 12 weeks old showed high seroprevalences (70-100%), while pigs aged 10-12 weeks showed no detectable serum antibodies levels. Our results suggest that PES infections are common in pigs and that passively acquired maternal antibodies are soon replaced by actively acquired antibodies, whose titers increase gradually with age and that probably are maintained during lifetime.
authors.affiliation: Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Alcalá
authors.forename: Jesús
authors.initials: J
authors.lastname: Rodríguez-Díaz
authors.forename: Morella
authors.initials: M
authors.lastname: de Rolo
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Vizzi
authors.forename: Javier
authors.initials: J
authors.lastname: Buesa
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Liprandi
authors.forename: Juan E
authors.initials: JE
authors.lastname: Ludert
mesh_terms: D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016367:Baculoviridae / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D029341:Sapovirus / Q000737:chemistry* / Q000302:isolation & purification; D016036:Seroepidemiologic Studies; D013552:Swine / Q000821:virology*; D014771:Virion / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins