title: miR-126 functions as a tumour suppressor in human gastric cancer.
abstract: MicroRNAs have emerged as important gene regulators and are recognised as key players in carcinogenesis. In the present study, we show that miR-126 was significantly down-regulated in gastric cancer tissues compared with matched normal tissues and was associated with clinicopathological features, including tumour size, lymph node metastasis, local invasion and tumour-node-metastasis (TNM) stage. Ectopic expression of miR-126 in SGC-7901 gastric cancer cells potently inhibited cell growth by inducing cell cycle arrest in G0/G1 phase, migration and invasion in vitro as well as tumorigenicity and metastasis in vivo. Mechanistically, we identified the adaptor protein Crk as a target of miR-126. Taken together, our results suggest that miR-126 may function as a tumour suppressor in gastric cancer, with Crk as a direct target.
authors.affiliation: Department of Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui Jin Er Road, Shanghai 20025, People's Republic of China.
authors.forename: Runhua
authors.initials: R
authors.lastname: Feng
authors.forename: Xuehua
authors.initials: X
authors.lastname: Chen
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Liping
authors.initials: L
authors.lastname: Su
authors.forename: Beiqin
authors.initials: B
authors.lastname: Yu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Li
authors.forename: Qu
authors.initials: Q
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D050737:Proto-Oncogene Proteins c-crk / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
chemical_list: C485285:CRK protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D050737:Proto-Oncogene Proteins c-crk