SELDI-TOF serum proteomics and colorectal cancer: a current overview.
Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use.
Gemoll
Timo
T
Laboratory for Surgical Research, Department of Surgery, University Clinic Schleswig-Holstein, Campus Lübeck, D-23538 Lübeck, Germany.
Roblick
Uwe Johannes
UJ
Auer
Gert
G
Jörnvall
Hans
H
Habermann
Jens Karsten
JK
D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000097:blood / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D015415:Biomarkers; D001798:Blood Proteins; D009363:Neoplasm Proteins