title: Functional F11L and K1L genes in modified vaccinia virus Ankara restore virus-induced cell motility but not growth in human and murine cells.
abstract: Modified vaccinia virus Ankara (MVA) was generated by serial passaging in chicken embryo fibroblasts. During this attenuation, MVA lost the capacity to productively grow in human and most other mammalian cell lines, as well as acquiring a multitude of deletions and mutations in the MVA genome. This means that the precise molecular basis for the MVA host-range restriction is still unknown. The vaccinia virus (VACV) genes F11L and K1L are mutated or truncated in MVA. F11L was previously implicated in VACV-induced cell motility and virion maturation. Here, we demonstrate that the restoration of F11L gene expression in MVA rescued virus-induced cell motility, but had no impact on MVA virion maturation and host-range restriction. Additional insertion of the K1L gene, which restores MVA replication in RK-13 cells, was not sufficient to extend MVA growth capacity to other mammalian cells.
authors.affiliation: Abteilung für Virologie, Paul-Ehrlich Institut, 63225 Langen, Germany. zwijo@pei.de
authors.forename: Joachim
authors.initials: J
authors.lastname: Zwilling
authors.forename: Katja
authors.initials: K
authors.lastname: Sliva
authors.forename: Astrid
authors.initials: A
authors.lastname: Schwantes
authors.forename: Barbara
authors.initials: B
authors.lastname: Schnierle
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D002642:Chick Embryo; D006224:Cricetinae; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009154:Mutation; D011817:Rabbits; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000502:physiology*
chemical_list: C533030:F11L protein, vaccinia virus; C083205:K1L protein, Vaccinia virus; D014764:Viral Proteins