[Study on the anti-NTHi infection of Hap recombinant protein in vivo].
OBJECTIVE
To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi, and explore the mechanism about the anti-NTHi infection.


METHODS
The C57BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads. The immunifaction of anti-infection was observed through encocyte counting of BALF, bacteria detection of lung and the pathologyical change of lung tissue.


RESULTS
In the challenge with NTHi experiment, the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B, and quantity of NTHi in lung of the infected murine was reduced obviously.


CONCLUSIONS
The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia. This study could offer the oretical and experimental basis for development of new vaccine against NTHi.
Li
Wan-yi
WY
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. hxliwan@126.com
Wang
Bao-ning
BN
Zuo
Feng-qiong
FQ
Zeng
Wei
W
Feng
Feng
F
Kuang
Yu
Y
Jiang
Zhong-hua
ZH
Li
Ming-yuan
MY
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006192:Haemophilus Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D006193:Haemophilus influenzae / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007114:Immunization; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C091552:Hap protein, Hemophilus influenzae; D012697:Serine Endopeptidases