title: Modules for C-terminal epitope tagging of Tetrahymena genes.
abstract: Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.
authors.affiliation: Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohr-Gasse 3, A-1030 Vienna, Austria.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kataoka
authors.forename: Ursula E
authors.initials: UE
authors.lastname: Schoeberl
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Mochizuki
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D013768:Tetrahymena / Q000737:chemistry / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins