[Level of Th17 cell and CD4(+);CD25(+); Foxp3(+); regulatory T cell in peripheral blood mononuclear cells of primary nephrotic syndrome in children].
OBJECTIVE
To investigate the levels and functions of Th17 cells and CD4(+); CD25(+); Foxp3(+); regulate cells(Treg) and explore their role in pathogenesis of primary nephrotic syndrome (PNS) in children.


METHODS
Children with PNS were divided into simple type nephritic syndrome group (SNS) (n = 20), and nephritic type nephritic syndrome group (NNS) (n = 15). 20 healthy subjects were selected as control group. The circulating frequencies of Th17 cells and Treg were measured by FCM. Real-time PCR were used to analyze the mRNA expressions of RORC, IL-23p19 and Foxp3 in peripheral blood mononuclear cells. The serum of IL-1ß, IL-6, TGF-ß1 were measured by ELISA.


RESULTS
Circulating frequencies of Th17 cells, the mRNA levels of RORC, IL-23p19 and the serum of IL-1ß, IL-6 were higher in SNS and NNS groups than control group (P < 0.05), and they were higher in NNS group than SNS group (P < 0.05). Compared with control group, the circulating frencencies of Treg cells, the Foxp3mRNA levels and the serum levels of TGF-ß1 were lower in NNS and SNS groups (P < 0.05), and the circulating frencencies of Treg cells, the Foxp3mRNA levels were lower in NNS group than SNS group (P < 0.05), but NNS and SNS group had no statistical difference in the serum levels of TGF-ß1 (P > 0.05).


CONCLUSIONS
Imbalance of Th17 and Treg cells might contribute to the pathogenesis of PNS in children and have associated with clinical presentation, pathological type, glucocorticoid sensitivity and prognosis of the disease.
Wang
Li
L
Department of Nephroimmunology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
Li
Qiu
Q
Wang
Li-jia
LJ
Li
Xin
X
D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D009404:Nephrotic Syndrome / Q000235:genetics / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit