Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions.
Chromatography technology was employed to clarify the mechanism of interaction between multi-wall carbon nanotubes (MWCNT) and proteins. A column (16x100 mm) was packed with purified MWCNT, and various proteins were eluted with phosphate buffered saline (PBS) with and without gradient systems. It was found that albumin in bovine serum was eluted immediately from the column without any adsorption to MWCNT. Conversely, the non-albumin proteins, including a protein of 85 kDa molecular mass and a group of proteins with molecular masses higher than 115 kDa, exhibited considerably high affinity towards MWCNT. A sample of pure bovine serum albumin was also eluted immediately from the column, while lysozyme did not elute as a peak with PBS, but eluted with 0.6 M NaCl. Fundamentally, carbon nanotubes are devoid of any electrical charge. Therefore, other forces including the hydrogen bonds, hydrophilic interactions, and van der Waals forces were most probably responsible for the differential elution behaviors. In conclusion, this chromatographic method provided a simple and direct analysis of the interactions between carbon nanotubes and the various proteins.
Kuboki
Yoshinori
Y
Hokkaido University, Kita-ku, Sapporo, Japan. kuboki.yoshinori@gmail.com
Koshikawa
Takamitu
T
Takita
Hiroko
H
Fujisawa
Ryuichi
R
Lee
Min-ho
MH
Abe
Shigeaki
S
Akasaka
Tsukasa
T
Uo
Motohiro
M
Watari
Fumio
F
Sammons
Rachel
R
D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D002417:Cattle; D055598:Chemical Phenomena; D002845:Chromatography / Q000295:instrumentation*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006860:Hydrogen Bonding; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010710:Phosphates; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D012965:Sodium Chloride; D017155:Wettability
D001798:Blood Proteins; D002021:Buffers; D037742:Nanotubes, Carbon; D010710:Phosphates; D012709:Serum Albumin; D012965:Sodium Chloride; D009113:Muramidase