title: Effects of mycotoxins on chemiluminescent response and cytokine mRNA expression of bovine neutrophils.
abstract: The effects of aflatoxin B₁ (AFB₁), aflatoxin M₁ (AFM₁), deoxynivalenol (DON) and zearalenone (ZEA) on the viability, chemiluminescent (CL) response and expression of cytokine mRNA of bovine neutrophils (PMNs) were evaluated. The opsonized zymosan (OPZ)-stimulated CL response of PMNs was significantly (P < 0.05) decreased by AFB₁ ( > 50 pg/ml), AFM₁ ( > 50 pg/ml) and ZEA (>50 pg/ml). The phorbol myristate acetate (PMA)-stimulated CL response PMNs was significantly (P < 0.05) decreased by AFB₁ (> 0.5 pg/ml), AFM₁ (> 50 pg/ml), ZEA (> 500 pg/ml) and DON ( > 5 pg/ml). Treatment with AFB₁ resulted in reduction in the mRNA expression of interleukin-1β and tumor necrosis factor-α of PMNs stimulated with OPZ and PMA. These results suggest that these four mycotoxins have inhibitory effects on the function of bovine PMNs.
authors.affiliation: Yamagata Prefecutural Federation of Agricultural Mutual Aid Associations, Yamagata, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Wada
authors.forename: Akiko
authors.initials: A
authors.lastname: Nabae
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Nagahata
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology; D016607:Aflatoxin M1 / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D007700:Kinetics; D049449:Luminescence; D009183:Mycotoxins / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D014255:Trichothecenes / Q000494:pharmacology; D015025:Zearalenone / Q000494:pharmacology
chemical_list: D016207:Cytokines; D009183:Mycotoxins; D012333:RNA, Messenger; D014255:Trichothecenes; D015025:Zearalenone; D016607:Aflatoxin M1; D016604:Aflatoxin B1; C007262:deoxynivalenol