title: Effect of chlorogenic acid on mast cell-dependent anaphylactic reaction.
abstract: Chlorogenic acid (CGA), a naturally occurring polyphenol compound, has a number of biological activities. However, roles of CGA in the mast cell-dependent anaphylactic reaction have not been fully examined. In the present study, the effect and mechanism of CGA on mast cell-dependent anaphylactic reaction were investigated using in vivo and in vitro models. CGA inhibited compound 48/80-induced systemic anaphylactic shock in mice and skin vascular permeability in rats. CGA also inhibited anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis (PCA). Moreover, CGA dose-dependently reduced histamine and TNF-alpha release from RBL-2H3 cells activated by anti-DNP IgE. Pretreatment with CGA suppressed IgE-antigen complex induced calcium uptake into RBL-2H3 cells. When CGA was added, the level of intracellular cyclic adenosine monophosphate (cAMP) in RBL-2H3 cells was significantly elevated compared with the untreated cells. Decreased calcium uptake and increased cAMP level might be involved in the inhibitory effect of CGA on mast cell activation. These results suggest a possible therapeutic application of CGA in allergic diseases.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44(#) Wen Hua Xi Road, Jinan, 250012, PR China.
authors.forename: Hui-Di
authors.initials: HD
authors.lastname: Qin
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Shi
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Zhong-Gang
authors.initials: ZG
authors.lastname: Li
authors.forename: Huai-Shan
authors.initials: HS
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Zhao-Ping
authors.initials: ZP
authors.lastname: Liu
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000276:immunology; D015220:Calcium Channels / Q000187:drug effects / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D000242:Cyclic AMP / Q000032:analysis / Q000276:immunology; D004140:Dinitrophenols / Q000633:toxicity; D006636:Histamine Release / Q000187:drug effects / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine / Q000009:adverse effects / Q000276:immunology
chemical_list: D015220:Calcium Channels; D004140:Dinitrophenols; D014409:Tumor Necrosis Factor-alpha; D002726:Chlorogenic Acid; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D002118:Calcium