Development of a UPLC-ESI-MS/MS assay for 20(S)-protopanaxadiol and pharmacokinetic application of its two formulations in rats.
An ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed to investigate 20(S)-protopanaxadiol (PPD) pharmacokinetics in rats. Rat plasma samples were treated using a solid-phase extraction with satisfactory recovery (> 81%). The method showed an excellent sensitivity that the limit of detection (LOD) and the lower limit of quantitation (LLOQ) of PPD were 0.5 and 2 ng/mL, respectively. The method was applied to the evaluation of pharmacokinetics from two types of PPD formulations. The PPD emulsion showed more rapid and efficient drug absorption, and higher and more persistent plateau concentration of PPD in plasma than PPD oil solution. PPD emulsion was demonstrated to be a promising dosage form. In spite of lower plateau plasma drug concentration, PPD oil solution was characterized by the easiness in preparation and the persistent, durative plateau plasma concentration of PPD, there is room to further improve its bioavailability.
Han
Meihua
M
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing 100193, P. R. China.
Chen
Jing
J
Chen
Shilin
S
Wang
Xiangtao
X
D000818:Animals; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012502:Sapogenins / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D012502:Sapogenins; C062916:protopanaxadiol