[Studies on mechanism of myricetin-induced apoptosis in human hepatocellular carcinoma HepG-2 cells].
OBJECTIVE
To study the mechanism of myricetin inducing the HepG-2 cell line apoptosis.


METHODS
The MTT method was employed to study myricetin pharmacodynamics in HepG-2. The light microscope and transmission was used to identify the tumor cell apoptosis in the morphology. The FCM method and the kit of caspase 3, caspase 9 were hired to detect the apoptosis rates, the content of mitochondrial membrane electric potential and the activity of caspase in cancer cells.


RESULTS
Myricetin significantly inhibits the proliferation and induces the apoptosis of HepG-2 in a dose-dependent manner, which is accompanied with G2/M and S phase arrest. In addition, myricetin also increases the activation of caspase 3,9 and results in a depolarization and delta psi m collapse in a dose-dependent manner.


CONCLUSIONS
The molecular pathway of apoptosis of human hepatocellular carcinoma cell lines induced by myricetin might deal with the mitochondria-mediated pathway.
Zhang
Xiujuan
X
Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. zxj1717@yahoo.com.cn
Ling
Yun
Y
Yu
Hua
H
Ji
Yubin
Y
D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects
D005419:Flavonoids; C040015:myricetin