title: [Study on the anti-NTHi infection of Hap recombinant protein in vivo].
abstract: OBJECTIVE
  To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi, and explore the mechanism about the anti-NTHi infection.
  
  
  METHODS
  The C57BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads. The immunifaction of anti-infection was observed through encocyte counting of BALF, bacteria detection of lung and the pathologyical change of lung tissue.
  
  
  RESULTS
  In the challenge with NTHi experiment, the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B, and quantity of NTHi in lung of the infected murine was reduced obviously.
  
  
  CONCLUSIONS
  The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia. This study could offer the oretical and experimental basis for development of new vaccine against NTHi.
authors.affiliation: Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. hxliwan@126.com
authors.forename: Wan-yi
authors.initials: WY
authors.lastname: Li
authors.forename: Bao-ning
authors.initials: BN
authors.lastname: Wang
authors.forename: Feng-qiong
authors.initials: FQ
authors.lastname: Zuo
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Yu
authors.initials: Y
authors.lastname: Kuang
authors.forename: Zhong-hua
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Li
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006192:Haemophilus Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D006193:Haemophilus influenzae / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007114:Immunization; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D012697:Serine Endopeptidases / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C091552:Hap protein, Hemophilus influenzae; D012697:Serine Endopeptidases