Determination of indoxacarb enantiomer residues in vegetables, fruits, and soil by high-performance liquid chromatography.
An effective chiral analytical method was developed for the resolution and determination of indoxacarb enantiomers in cucumber, tomato, apple, pear, and soil samples. Indoxacarb enantiomers were separated on a Chiralpak AS-H column with n-hexane-ethanol (95 + 5, v/v) as the mobile phase. Validation involved complete resolution of each enantiomer, plus determination of linearity, precision, LOD, and LOQ. The estimated LOD was 0.025-0.035 mg/kg, and the LOQ was 0.05 mg/kg for each indoxacarb enantiomer in different matrixes. The average recoveries of the pesticide from all matrixes ranged from 87.0 to 116.9% for fortification levels of 0.05, 0.1, and 5 mg/kg. The precision values associated with the analytical method, expressed as RSD(r) values, were < 10.1% for the pesticide in all matrixes. This method can be used to evaluate environmental residues and the safety of agricultural products.
Cheng
Li
L
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Beijing 100193, China.
Dong
Feng-Shou
FS
Liu
Xingang
X
Chen
Wuwing
W
Li
Yuanbo
Y
Zheng
Yongquan
Y
Qin
Dongmei
D
Gong
Yong
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D057230:Limit of Detection; D010078:Oxazines / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012987:Soil / Q000032:analysis*; D052616:Solid Phase Extraction; D013237:Stereoisomerism; D014675:Vegetables / Q000737:chemistry*
D010078:Oxazines; D010573:Pesticide Residues; D012987:Soil; C401104:indoxacarb