title: Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells.
abstract: Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound, doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.
authors.affiliation: Division of Biotechnology/IFM, Linköping University, 581 83 Linköping, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Andersson
authors.forename: Daniella
authors.initials: D
authors.lastname: Steel
authors.forename: Julia
authors.initials: J
authors.lastname: Asp
authors.forename: Kerstin
authors.initials: K
authors.lastname: Dahlenborg
authors.forename: Marianne
authors.initials: M
authors.lastname: Jonsson
authors.forename: Anders
authors.initials: A
authors.lastname: Jeppsson
authors.forename: Anders
authors.initials: A
authors.lastname: Lindahl
authors.forename: Bertil
authors.initials: B
authors.lastname: Kågedal
authors.forename: Peter
authors.initials: P
authors.lastname: Sartipy
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Mandenius
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002452:Cell Count; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000378:metabolism*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006331:Heart Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D020349:Surface Plasmon Resonance; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D020107:Troponin T / Q000378:metabolism
chemical_list: D015415:Biomarkers; C493408:FABP3 protein, human; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D020107:Troponin T; D004317:Doxorubicin