Influence of polyphenolic extracts from Enydra fluctuans on oxidative stress induced by acephate in rats.
Acephate, an organophosphorus pesticide, has been proved to play an important role in tissue damage by inducing oxidative stress through the release of free radicals. The aim of this study was to evaluate the protective effect of the plant phenolic compounds present in Enydra fluctuans against acephate toxicity based on lipid peroxidation and antioxidant enzymes profile in rats. An oral dose of acephate at 30 mg/kg of body weight has been given against the extracts containing 20mg of polyphenolic compounds (expressed as gallic acid equivalents)/kg body weight for 14 days. The results showed that under the influence of the pesticides, there was significant decrease in the activities of the antioxidant enzymes SOD, Catalase and Glutathione peroxidase (GPx) and an increase in the non-enzymatic Glutathione, with respect to the normal and the plant extract gavaged groups. Also that there was an increase in the plasma and erythrocyte membrane lipid peroxidation levels in the pesticide treated group compared to the normal or the group treated with the plant extract. The present study thus gives an insight into the ill-effects of this organophosphate and the protective role of plant polyphenols in minimizing those effects.
Datta
Sanjukta
S
Department of Chemical Technology, University College of Science and Technology, University of Calcutta, 92 APC Road, Kolkata 700 009, India. sanj_9231@yahoo.co.in
Dhar
Pubali
P
Mukherjee
Aniruddha
A
Ghosh
Santinath
S
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D007306:Insecticides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009946:Organothiophosphorus Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D063088:Phosphoramides; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005419:Flavonoids; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D010636:Phenols; D063088:Phosphoramides; D010936:Plant Extracts; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances; C001969:acephate