A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein.
A new kind of aptamer-based immunomagnetic electrochemiluminescence (IM-ECL) assay for quantitative detection of protein is developed. The assay consists of a double aptamer sandwich format in which a biotin-labeled aptamer is used for rapid and specific capture of target protein, and a [Ru(bpy)(3)](2+) (TBR)-labeled aptamer is used for ECL detection. As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method. Experimental results show that the detection limit of the assay is 80 pmol/L of PDGF-BB. A calibration curve with a linearity range from 0.1 to 1000 nmol/L is established, thus, make quantitative analysis possible. The method has been used to detect PDGF-BB in fetal calf serum with minimum background interference. Due to the wide availability of aptamer for numerous proteins, this new method holds great promise in protein detection.
Zhu
Debin
D
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Zhou
Xiaoming
X
Xing
Da
D
D052158:Aptamers, Peptide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D052158:Aptamers, Peptide; D011506:Proteins