title: Disruption of the membrane-bound alcohol dehydrogenase-encoding gene improved glycerol use and dihydroxyacetone productivity in Gluconobacter oxydans.
abstract: Dihydroxyacetone (DHA) production from glycerol by Gluconobacter oxydans is an industrial form of fermentation, but some problems exist related to microbial DHA production. For example, glycerol inhibits DHA production and affects its biological activity. G. oxydans produces both DHA and glyceric acid (GA) from glycerol simultaneously, and membrane-bound glycerol dehydrogenase and membrane-bound alcohol dehydrogenases are involved in the two reactions, respectively. We discovered that the G. oxydans mutant DeltaadhA, in which the membrane-bound alcohol dehydrogenase-encoding gene (adhA) was disrupted, significantly improved its ability to grow in a higher concentration of glycerol and to produce DHA compared to a wild-type strain. DeltaadhA grew on 220 g/l of initial glycerol and produced 125 g/l of DHA during a 3-d incubation, whereas the wild-type did not. Resting DeltaadhA cells converted 230 g/l of glycerol aqueous solution to 139.7 g/l of DHA during a 3-d incubation. The inhibitory effect of glycerate sodium salt on DeltaadhA was investigated. An increase in the glycerate concentration at the beginning of growth resulted in decreases in both growth and DHA production.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST). hiroshi.habe@aist.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Keiji
authors.initials: K
authors.lastname: Sakaki
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D002462:Cell Membrane / Q000201:enzymology*; D004098:Dihydroxyacetone / Q000096:biosynthesis*; D020600:Gluconobacter oxydans / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005988:Glyceric Acids / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation
chemical_list: D005988:Glyceric Acids; C042971:glyceric acid; D000426:Alcohol Dehydrogenase; D004098:Dihydroxyacetone; D005990:Glycerol