title: Array comparative genomic hybridization analysis revealed four genomic prognostic biomarkers for primary gastric cancers.
abstract: Unlike the case with some other solid tumors, whole genome array screening has not revealed prognostic genetic aberrations in primary gastric cancer. Comparative genomic hybridization (CGH) using bacterial artificial chromosome (BAC) arrays for 56 primary gastric cancers resulted in identification of four prognostic loci in this study: 6q21 (harboring FOXO3A; previously FKHRL1), 9q32 (UGCG), 17q21.1 approximately q21.2 (CASC3), and 17q21.32 (HOXB3 through HOXB9). If any one of these four loci was deleted, the prognosis of the patient was significantly worse (P = 0.0019). This was true even for advanced tumors (stage IIIA, IIB, or IV, n = 39) (P = 0.0113), whereas only 1 of the 17 patients with less advanced tumors (stage IA, IB, or II; n = 17) died of disease after surgery. Multivariate analysis according to the status of four BACs or pathological stage based on the Japanese Classification of Gastric Carcinoma (stages IA, IB, and II vs. stages IIIA, IIIB, and IV) demonstrated that the BAC clone status was also an independent prognostic factor (P = 0.006). These findings may help predict which patients with malignant potential need more intensive therapy, or may point to new therapeutic approaches especially for advanced tumors. The parameter here termed the integrated genomic prognostic biomarker may therefore be of clinical utility as a prognostic biomarker.
authors.affiliation: Department of General Surgery, Hokkaido University Graduate School of Medicine, N-15 W-7 Kita-ku, Sapporo, Japan. ntomioka@sapporo-shaho.jp
authors.forename: Nobumoto
authors.initials: N
authors.lastname: Tomioka
authors.forename: Keiko
authors.initials: K
authors.lastname: Morita
authors.forename: Nozomi
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Tada
authors.forename: Tomoo
authors.initials: T
authors.lastname: Itoh
authors.forename: Soichiro
authors.initials: S
authors.lastname: Saitoh
authors.forename: Masao
authors.initials: M
authors.lastname: Kondo
authors.forename: Norihiko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kataoka
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Masato
authors.initials: M
authors.lastname: Takahashi
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kamiyama
authors.forename: Michitaka
authors.initials: M
authors.lastname: Ozaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.forename: Satoru
authors.initials: S
authors.lastname: Todo
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers