title: Increased expression of ganglioside GM1 in peripheral CD4+ T cells correlates soluble form of CD30 in Systemic Lupus Erythematosus patients.
abstract: Gangliosides GM1 is a good marker of membrane microdomains (lipid rafts) with important function in cellular activation processes. In this study we found that GM1 expression on CD4+ T cells and memory T cells (CD45RO/CD4) were dramatic increased after stimulation with phytohaemagglutinin in vitro. Next, we examined the GM1 expression on peripheral blood CD4+ T cells and CD8+ T cells from 44 patients with SLE and 28 healthy controls by flow cytometry. GM1 expression was further analyzed with serum soluble CD30 (sCD30), IL-10, TNF-alpha and clinical parameters. The mean fluorescence intensity of GM1 on CD4+ T cells from patients with SLE was significantly higher than those from healthy controls, but not on CD8+ T cells. Increased expression of GM1 was more marked on CD4+/CD45RO+ memory T cells from active SLE patients. Patients with SLE showed significantly elevated serum sCD30 and IL-10, but not TNF-alpha levels. In addition, we found that enhanced GM1 expression on CD4+ T cells from patients with SLE positively correlated with high serum levels of sCD30 and IgG as well as disease activity (SLEDAI scores). Our data suggested the potential role of aberrant lipid raft/GM1 on CD4+ T cells and sCD30 in the pathogenesis of SLE.
authors.affiliation: Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. dongll@tjh.tjmu.edu.cn
authors.forename: Lingli
authors.initials: L
authors.lastname: Dong
authors.forename: Shaoxian
authors.initials: S
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Lei
authors.forename: Wei
authors.initials: W
authors.lastname: Tu
authors.forename: Yikai
authors.initials: Y
authors.lastname: Yu
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Takuro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Masaki
authors.forename: Hisanori
authors.initials: H
authors.lastname: Umehara
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005677:G(M1) Ganglioside / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007156:Immunologic Memory / Q000187:drug effects; D016753:Interleukin-10 / Q000097:blood; D017730:Ki-1 Antigen / Q000097:blood / Q000276:immunology*; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D021962:Membrane Microdomains / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D012995:Solubility / Q000187:drug effects; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D007074:Immunoglobulin G; D017730:Ki-1 Antigen; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D005677:G(M1) Ganglioside