[Effects of electroacupuncture on glucose transporter-1 expression of hippocampal microvascular endothelial cells in rats with focal cerebral ischemia].
OBJECTIVE
To observe the effect of electroacupuncture (EA) on the expression of glucose transporter-i (GLUT-1) of hippocampal microvascular endothelial cells in focal cerebral ischemia (CI) rats.


METHODS
Sixty male Wistar rats were randomly divided into sham-operation, model and electroacupuncture (EA) groups. CI model was established by middle cerebral artery occlusion with a piece of thread embolus. EA (4 Hz/20 Hz, 1 mA) was applied to bilateral "Neiguan" (PC 6) for 30 min, once daily for 15 days. GLUT-1 expression in the hippocampus was detected with immunohistochemistry and Western blot, respectively.


RESULTS
After the treatment, compared with the sham-operation group, the expression of GLUT-1 in microvascular endothelial cells of the hippocampus in model group shown by both histochemical and Western blot techniques decreased apparently (P < 0.05). While in comparison with model group, the expression of GLUT-1 in EA group was upregulated significantly (P < 0.05).


CONCLUSIONS
EA of PC 6 can effectively upregulate GLUT-1 expression in the hippocampus in cerebral ischemia rats, which may contribute to its effect in improving CI by raising the glucose transportation of microvascular endothelial cells.
Lu
Yan
Y
College of Acu-moxibustion and Massage, Shandong University of Chinese Medicine, Jinan 250355, China.
Wang
Shi-jun
SJ
Song
Xiu-tao
XT
D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D004195:Disease Models, Animal; D015671:Electroacupuncture; D042783:Endothelial Cells / Q000378:metabolism*; D015870:Gene Expression; D051272:Glucose Transporter Type 1 / Q000235:genetics* / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D051272:Glucose Transporter Type 1