Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles.
The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37°C for 6h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL).
Sener
Gulsu
G
Hacettepe University, Department of Chemistry, Ankara, Turkey.
Ozgur
Erdogan
E
Yılmaz
Erkut
E
Uzun
Lokman
L
Say
Ridvan
R
Denizli
Adil
A
D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D009113:Muramidase / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000295:instrumentation*; D013499:Surface Properties
D009113:Muramidase