The rolD oncogene promotes axillary bud and adventitious root meristems in Arabidopsis.
The expression of the Agrobacterium rhizogenes rolD oncogene induces precocious floral transition and strong flowering potential in tobacco and tomato. Here, we describe specific developmental effects induced by expression of rolD in Arabidopsis. We show that floral transition, as histologically monitored, occurred in rolD- plants earlier than in wild type, and this was coupled with a premature and enhanced formation of vegetative and reproductive axillary bud meristems. Furthermore, CYP79F1/SUPERSHOOT/BUSHY (SPS), a gene that negatively controls shoot branching in Arabidopsis and involved in glucosinolate metabolism and in cytokinin and auxin homeostasis, was down-regulated in rolD plants. The multiplication of post-embryonic meristems was also observed in the root system, with enhanced adventitious root formation. This result was confirmed by thin cell layer response in vitro, both under hormone-free and standard rooting conditions. However, the formation of lateral root meristems was not affected by rolD expression. Our results show that rolD accelerates and enhances specific post-embryonic meristems in Arabidopsis.
Falasca
Giuseppina
G
Dipartimento di Biologia Vegetale, Sapienza Università di Roma, 00185 Rome, Italy.
Altamura
Maria Maddalena
MM
D'Angeli
Simone
S
Zaghi
Daniela
D
Costantino
Paolo
P
Mauro
Maria Luisa
ML
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics
D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System; C089817:cytochrome P450TYR