title: Characterization of a cryptic plasmid pPZZ84 from Bacillus pumilus.
abstract: The complete nucleotide sequence of a cryptic plasmid pPZZ84 from Bacillus pumilus strain ZZ84 was determined. Plasmid pPZZ84 is 6817bp long with GC content of 36.7%. Seven putative open reading frames were identified. ORF7 shows 91% and 90% amino acid identity with rep proteins of pSH1452 and pPL1, respectively, members of rolling-circle replication (RCR) pC194-family. A typical pC194-family double strand origin (dso), a single-stranded origin (sso) and rap (regulator aspartate phosphatase) proteins were also identified in the plasmid. These results imply that pPZZ84 belongs to the Bacillus subtilis species group of small rolling circle (BsSRC) replicating plasmids. The plasmid copy number of pPZZ84 in B. pumilus ZZ84 was estimated to be 46 per cell, more than that of other BsSRC plasmids in their hosts.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.forename: Dong-Yang
authors.initials: DY
authors.lastname: Liu
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Liu
authors.forename: Qi-Rong
authors.initials: QR
authors.lastname: Shen
authors.forename: Biao
authors.initials: B
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D004269:DNA, Bacterial