Quantitation of fatty acid ethyl esters in human meconium by an improved liquid chromatography/tandem mass spectrometry.
This paper reports the development and validation of an improved assay for quantitation of fatty acid ethyl esters (FAEEs) in human meconium using liquid chromatography/tandem mass spectrometry (LC-MS/MS). FAAEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate, ethyl linolenate, and ethyl arachidonate) and the internal standard (I.S.), ethyl heptadecanoate, were separated by reverse phase HPLC and quantified by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the positive ionization mode. The absolute recovery of FAEEs varied from 55+/-10% for 0.33 nmol/g (100 ng/g) of ethyl linoleate up to 86+/-8% for 1.55 nmol/g (500 ng/g) of ethyl miristate. The LODs and LOQs varied from 0.01 to 0.08 nmol/g and from 0.02 to 0.27 nmol/g, respectively. The assay has been successfully applied to examine the FAEE levels in 81 meconium samples from babies born to mothers reporting alcohol consumption, to varying degrees, during pregnancy.
Kwak
Ho-Seok
HS
Division of Endocrinology, Department of Internal Medicine, Cheil General Hospital and Women's Healthcare Center, Kwandong University School of Medicine, Seoul, Republic of Korea.
Kang
Young-Sun
YS
Han
Ki-Ok
KO
Moon
Jong-Taek
JT
Chung
Young-Chul
YC
Choi
June-Seek
JS
Han
Jung-Yeol
JY
Kim
Moon-Young
MY
Velázquez-Armenta
E Yadira
EY
Nava-Ocampo
Alejandro A
AA
D000437:Alcoholism; D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D008470:Meconium / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004952:Esters; D005227:Fatty Acids