Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization.
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 microM, 0.96 microM and 18 nM, respectively.
Rakthong
Pakavadee
P
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Intaramat
Akarin
A
Ratanabanangkoon
Kavi
K
D000918:Antibody Specificity / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D019921:Biotinylation; D005240:Feasibility Studies; D055767:Immobilized Proteins / Q000737:chemistry; D008027:Light; D008081:Liposomes / Q000737:chemistry*; D008165:Luminol / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012542:Scattering, Radiation; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
D000936:Antigen-Antibody Complex; D055767:Immobilized Proteins; D008081:Liposomes; D009841:Oligonucleotides; D010714:Phosphatidylethanolamines; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D008165:Luminol; D019809:Streptavidin