title: Synthesis and properties of fluorescence probe for detection of peroxides in mitochondria.
abstract: In this study, a new type of fluorescence probe, diphenylpyrenylphosphine-conjugated alkyltriphenylphosphonium iodide which was accumulated in mitochondria, has been synthesized. This probe was detected peroxide in living cell. Comparison of the reactivity toward several peroxide indicated that the probe was existed in mitochondrial membrane. Using this probe, generation of peroxide in mitochondria of living cell was successfully visualized.
authors.affiliation: Department of Chemistry, Fukuoka University, Jonan-Ku, Japan. shioji@fukuoka-u.ac.jp
authors.forename: Kosei
authors.initials: K
authors.lastname: Shioji
authors.forename: Yu
authors.initials: Y
authors.lastname: Oyama
authors.forename: Kentaro
authors.initials: K
authors.lastname: Okuma
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry*; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D010545:Peroxides / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D010545:Peroxides