title: Stearoyl-CoA desaturase (SCD) gene polymorphism in goat breeds.
abstract: There is evidence that stearoyl-CoA desaturase (SCD) is a key enzyme for lipid metabolism. Changes in enzyme activity that depends on SCD gene polymorphism and regulation could cause variations of fatty acid composition of meat and milk. We investigated genetic variability in caprine SCD, analyzing 335 animals belonging to three goat breeds by single-strand conformation polymorphism and DNA sequencing. Six single nucleotide polymorphism were identified, in intron 3 (585T-->A and 601A-->G; Ref. AF422168), intron 4 (719T-->A; AF422169), and exon 6 (690 A-->G, 718 C-->G, and 802 A-->C; AF422171). The less polymorphic SNP 601A-->G displayed variability only in Xuhuai and Boer breeds. Across breeds, the average frequency for the least frequent alleles ranged from 0.1158 to 0.2532. The three SNPs in exon 6 resulted in variations of amino acids 313 tyr --> cys, 322 phe --> leu, and 350 arg --> ser. The allelic distribution in exon 6 of Xuhuai was significantly different from Boer and Haimen.
authors.affiliation: Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, China.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Gao
authors.forename: Ru-Ying
authors.initials: RY
authors.lastname: Shao
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Xing-Tang
authors.initials: XT
authors.lastname: Fang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D006041:Goats / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D008460:Meat; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D013230:Stearoyl-CoA Desaturase