title: Antioxidant activity and cytotoxicity of the traditional Indonesian medicine Tahongai (Kleinhovia hospita L.) extract.
abstract: We investigated the leaves of Kleinhovia hospita, a plant which has been traditionally used in Indonesia as phytotherapy to cure liver disease, to describe antioxidant materials from plant sources. K. hospita leaves were extracted with methanol and further partitioned into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and their cytotoxicity on HepG2 liver cancer cells was determined by a MTT assay. The K. hospita leaf methanol extract showed strong antioxidant activity (96%) compared with vitamin C (98%) by the DPPH method and the measured activity from the subsequent extracts of the methanol extract were 48.9% for n-hexane, 74.0% for diethyl ether, 84.3% for ethyl acetate, and 77.1% for the residue. The MTT assay showed the cytotoxicity of the methanol extract on HepG2 cells at 14%, 76%, and 80% at concentrations of 50 microg/mL, 87.5 microg/mL, and 125 microg/mL, respectively. Leaf extracts of the medicinal plant K. hospita showed potent antioxidant activity and moderate cytotoxicity on HepG2 liver cancer cells.
authors.affiliation: Wood Chemistry Laboratory, Department of Forest Product Technology, Forestry Faculty, Mulawarman University, Samarinda, East Kalimantan, Indonesia. tangkearung@yahoo.com
authors.forename: Enos Tangke
authors.initials: ET
authors.lastname: Arung
authors.forename: Irawan Wijaya
authors.initials: IW
authors.lastname: Kusuma
authors.forename: Sri
authors.initials: S
authors.lastname: Purwatiningsih
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Roh
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
authors.forename: Soohyeon
authors.initials: S
authors.lastname: Jeon
authors.forename: Yong-Ung
authors.initials: YU
authors.lastname: Kim
authors.forename: Edi
authors.initials: E
authors.lastname: Sukaton
authors.forename: Joko
authors.initials: J
authors.lastname: Susilo
authors.forename: Yuli
authors.initials: Y
authors.lastname: Astuti
authors.forename: Britanto Dani
authors.initials: BD
authors.lastname: Wicaksono
authors.forename: Ferry
authors.initials: F
authors.lastname: Sandra
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D007214:Indonesia; D019660:Malvaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts