1H, 13C and 15N resonance assignments of the human mesencephalic astrocyte-derived neurotrophic factor.
Parkinson's disease (PD) is a chronic, progressive neurodegenerative disease, where dopaminergic cells die most prominently in the area of substantia nigra. Neurotrophic factors (NTFs) are secreted proteins, which upon binding to their target receptors trigger survival pathways to prevent neuronal loss. Recently discovered NTFs mesencephalic astrocyte-derived neurotrophic factor (MANF) and conserved dopamine neurotrophic factor (CDNF) most efficiently protect and repair the dopaminergic neurons in the animal 6-OHDA models of PD. However, the neuroprotective mechanism of MANF/CDNF is currently elusive. To this end, we have employed high-resolution NMR spectroscopy to determine three-dimensional structure of full-length human MANF in solution and characterized C-terminal domain as structural unit of MANF protein.
Hellman
Maarit
M
Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland. Maarit.Hellman@helsinki.fi
Peränen
Johan
J
Saarma
Mart
M
Permi
Perttu
P
D002247:Carbon Isotopes; D006801:Humans; D006859:Hydrogen; D009414:Nerve Growth Factors / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary
D002247:Carbon Isotopes; C099695:MANF protein, human; D009414:Nerve Growth Factors; D009587:Nitrogen Isotopes; D006859:Hydrogen