title: Validation of short-term handling and storage conditions for marrow and peripheral blood stem cell products.
abstract: BACKGROUND
  Allogeneic hematopoietic stem cell transplants from unrelated donors are routinely used in the treatment of patients with hematologic malignancies. These cellular products are often collected off-site and require transport from the collection site to transplantation centers. However, the effects of transport conditions and media on stem cell graft composition during short-term storage have not been well described.
  
  
  METHODS
  Five bone marrow (BM), four filgrastim-mobilized peripheral blood stem cell (PBSC), and four nonmobilized peripheral blood mononuclear cell (PBMNC) products were collected from healthy volunteer donors and stored at 4 or 20°C for up to 72 hours in 10% PlasmaLyte A plus anticoagulants such as 10% acid citrate dextran-A (ACD-A) and/or 10 IU/mL heparin. Products were evaluated at 0, 24, 48, and 72 hours for cellular content, viability, and metabolic activities.
  
  
  RESULTS
  BM products maintained equivalent cell viability when stored at either 4 or 20°C over 72 hours, but cell viability was better maintained for PBSC products stored at 4°C. The mean viable CD34+ cell recovery for PBSC and BM products stored over 72 hours at 4°C was higher than 75%. Significantly lower CD34+ cell and colony-forming unit recoveries were seen in PBSC products but not BM products stored at room temperature. Faster lactic acid accumulation was observed in PBMNC and PBSC products stored without ACD-A.
  
  
  CONCLUSIONS
  Seventy-two-hour storage of BM, PBSC, and PBMNC products at refrigerated temperature maintains optimal cell viability and recovery. Anticoagulation with ACD-A is preferred over heparin to reduce lactic acid accumulation in the product media.
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. grace_kao@dfci.harvard.edu
authors.forename: Grace S
authors.initials: GS
authors.lastname: Kao
authors.forename: Haesook T
authors.initials: HT
authors.lastname: Kim
authors.forename: Heather
authors.initials: H
authors.lastname: Daley
authors.forename: Jerome
authors.initials: J
authors.lastname: Ritz
authors.forename: Scott R
authors.initials: SR
authors.lastname: Burger
authors.forename: Linda
authors.initials: L
authors.lastname: Kelley
authors.forename: Cynthia
authors.initials: C
authors.lastname: Vierra-Green
authors.forename: Sue
authors.initials: S
authors.lastname: Flesch
authors.forename: Stephen
authors.initials: S
authors.lastname: Spellman
authors.forename: John
authors.initials: J
authors.lastname: Miller
authors.forename: Dennis
authors.initials: D
authors.lastname: Confer
mesh_terms: D002470:Cell Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous