A high prevalence of resistance in new tuberculosis cases of midwestern Brazil.
Tuberculosis (TB) is a serious worldwide public health problem, and drug resistance, particularly multidrug resistance (MDR), is a critical factor involved in TB control. We analyzed Mycobacterium tuberculosis isolates from 132 new TB cases of treatment-naïve patients in Goiás, Brazil by drug susceptibility tests, partial sequencing of the rpoB and katG genes, inhA(C-15T) mutation analysis by PCR, and RFLP-IS6110 genotyping. A high frequency of drug resistance was observed in previously untreated patients (13.6% to at least one antibiotic and 6.1% MDR-TB), and a high rate DNA polymorphism was detected in these strains. These results suggest that the prevalence of resistant TB is underestimated and that resistance in new TB cases was not associated with an outbreak in this region. We recommend routine culture and susceptibility testing for all new TB cases in Goiás for the appropriate treatment and control of this disease.
Santos
Lorena Cristina
LC
Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235 esq./1a Avenida, S/N, Setor Universitário, CEP 74605-050 Goiânia, Brazil. lorenacsantos@yahoo.com.br
Bousquet
Hesther de Macedo
Hde M
Pereira
Alyne Melo
AM
Junqueira-Kipnis
Ana Paula
AP
Kipnis
André
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000494:pharmacology*; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial; D054908:Extensively Drug-Resistant Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult
D000995:Antitubercular Agents