[The change of periphery and centra CD4(+);CD25(+);Treg, CD8(+);CD28(-);Treg in the MOG induced model of experimental autoimmune encephalomyelitis].
OBJECTIVE
To observe the change of periphery and centra CD4(+); CD25(+); Treg, CD8(+); CD28(-); Treg of MOG₃₅₋₅₅; induced EAE disease in mouse, and to explore the potential mechanism of cellular immunity in the process of EAE.


METHODS
MOG₃₅₋₅₅; were used to establish EAE model on femina C57BL/6 mice.The behavioral changes and the histological scores were recorded. The changes of CD4(+); CD25(+); Treg, CD8(+); CD28(-); Treg on periphery and centra lymphocyte in spleen , brain were analyzed by flow cytometry.


RESULTS
MOG₃₅₋₅₅;-induced EAE group Showed the typical clinical behavior and pathological manifestations, CD4(+); CD25(+); Treg, CD8(+); CD28(-); Treg lymphocytes were detected in the brain and spinal cord of EAE group mice, but they were not detected in the brain of control group. CD8(+); CD28(-); Treg in the spleen of EAE group were lower than those in control group (P < 0.01). CD4(+); CD25(+); Treg lymphocytes were slight higher than the control group.


CONCLUSIONS
CD4(+); CD25(+); Treg, CD8(+); CD28(-); Treg lymphocytes all play important roles in the pathogenesy of EAE. The distribution of CD4(+); CD25(+); Treg, CD8(+); CD28(-); Treg in the CNS and peripheral of EAE is different, suggesting that their entry into the CNS and regulate of local inflammation.
Shang
Xiao-feng
XF
Department of Neurology, the First Affiliated Hospital and Research Institute of Experimental Neurobiology, Wenzhou Medical College, Wenzhou 325000, China.
Xi
Na-na
NN
Wang
Tan
T
Lv
Jin
J
Han
Zhao
Z
Zheng
Rong-yuan
RY
D000818:Animals; D018106:CD28 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000009:adverse effects; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000009:adverse effects; D016176:T-Lymphocyte Subsets / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D018106:CD28 Antigens; D006023:Glycoproteins; D053645:Interleukin-2 Receptor alpha Subunit; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55)