title: Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their "stemness".
abstract: Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called "mesenchymal stem cells") to monitor their fate by in vivo MRI, and by histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitro differentiation of BMSCs, however, we chose to resolve the question of the effect of labeling on maintaining the "stemness" of cells within the BMSC population in vivo. Assays performed include colony forming efficiency, CD146 expression, gene expression profiling, and the "gold standard" of evaluating bone and myelosupportive stroma formation in vivo in immuncompromised recipients. SPION-labeling did not alter these assays. Comparable abundant bone with adjoining host hematopoietic cells were seen in cohorts of mice that were implanted with SPION-labeled or unlabeled BMSCs. PB+ adipocytes were noted, demonstrating their donor origin, as well as PB+ pericytes, indicative of self-renewal of the stem cell in the BMSC population. This study confirms that SPION labeling does not alter the differentiation potential of the subset of stem cells within BMSCs.
authors.affiliation: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, United States of America. balakumarana@nidcr.nih.gov
authors.forename: Arun
authors.initials: A
authors.lastname: Balakumaran
authors.forename: Edyta
authors.initials: E
authors.lastname: Pawelczyk
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Ren
authors.forename: Brian
authors.initials: B
authors.lastname: Sworder
authors.forename: Aneeka
authors.initials: A
authors.lastname: Chaudhry
authors.forename: Marianna
authors.initials: M
authors.lastname: Sabatino
authors.forename: David
authors.initials: D
authors.lastname: Stroncek
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Frank
authors.forename: Pamela G
authors.initials: PG
authors.lastname: Robey
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D051929:CD146 Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D052203:Ferrosoferric Oxide / Q000009:adverse effects / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D051929:CD146 Antigen; D052203:Ferrosoferric Oxide