title: Fractionation of persistent organic pollutants in fish oil by high-performance liquid chromatography on a 2-(1-pyrenyl)ethyl silica column.
abstract: The analysis of persistent organic pollutants in foodstuffs has become necessary for control of their levels in products for human and animal consumption. These analytical procedures usually require a fractionation step in order to separate the different families of pollutants to avoid interferences during the instrumental determination. In this study the separation was carried out on a 2-(1-pyrenyl)ethyl silica column, where analyte fractionation was based on differences in planarity and aromaticity. The fractionation of several types of persistent organic pollutants found in fish oil samples was studied; the pollutants included polychlorinated dibenzo-p-dioxins and dibenzofurans, polychlorinated biphenyls, polybrominated diphenyl ethers, and some organochlorine pesticides. Fractions were analyzed by high-resolution gas chromatography with electron-capture detection and high-resolution gas chromatography-high resolution mass spectroscopy. Finally, the whole method (including the purification, fractionation, and instrumental determination steps) was validated and successfully applied to the analysis of several samples of fish oil.
authors.affiliation: Analytical Chemistry Department, Institut Químic de Sarrià (Ramon Llull University), Via Augusta 390, 08017, Barcelona, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Ortiz
authors.forename: R
authors.initials: R
authors.lastname: Martí
authors.forename: M J
authors.initials: MJ
authors.lastname: Montaña
authors.forename: M
authors.initials: M
authors.lastname: Gasser
authors.forename: L
authors.initials: L
authors.lastname: Margarit
authors.forename: F
authors.initials: F
authors.lastname: Broto
authors.forename: J
authors.initials: J
authors.lastname: Díaz-Ferrero
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004147:Dioxins / Q000302:isolation & purification*; D005395:Fish Oils / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D010575:Pesticides / Q000302:isolation & purification*; D011075:Polybrominated Biphenyls / Q000302:isolation & purification*; D011078:Polychlorinated Biphenyls / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D004147:Dioxins; D005395:Fish Oils; D010575:Pesticides; D011075:Polybrominated Biphenyls; D012822:Silicon Dioxide; D011078:Polychlorinated Biphenyls