Single-molecule dual-beam optical trap analysis of protein structure and function.
Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.
Sung
Jongmin
J
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
Sivaramakrishnan
Sivaraj
S
Dunn
Alexander R
AR
Spudich
James A
JA
D009218:Myosins / Q000737:chemistry* / Q000378:metabolism*; D052898:Optical Tweezers
D009218:Myosins