title: Convergent synthesis of a helical, prehairpin HR1 trimer from HIV gp41.
abstract: A helical, prehairpin trimer covering the majority of the HR1 region of human immunodeficiency virus gp41 was achieved by chemically coupling three identical 51 amino acid peptides. A 1,3,5-tris(aminomethyl)-2,4,6-triethylbenzene pinwheel 'cap' was used to trimerize the peptides by taking advantage of the unique property of triacyl fluoride and orthogonal protection and deprotection. The resulting protein is fully helical, highly thermostable and soluble.
authors.affiliation: Trimeris, Inc., 3500 Paramount Parkway, Morrisville, NC 27560, USA. rjlu@frontierbiotech.com
authors.forename: Rong Jian
authors.initials: RJ
authors.lastname: Lu
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Mader
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Schneider
authors.forename: Nicolai
authors.initials: N
authors.lastname: Tvermoes
authors.forename: Myung-Choi
authors.initials: MC
authors.lastname: Kang
authors.forename: John J
authors.initials: JJ
authors.lastname: Dwyer
authors.forename: Karen L
authors.initials: KL
authors.lastname: Wilson
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Matthews
authors.forename: Mary K
authors.initials: MK
authors.lastname: Delmedico
authors.forename: Brian
authors.initials: B
authors.lastname: Bray
mesh_terms: D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments