Water-splitting manganese complex controls light-induced redox changes of cytochrome b559 in photosystem II.
The effect of water-splitting Mn complex on light-induced redox changes of cytochrome b(559) (cyt b(559)) was studied in spinach photosystem II (PSII) membranes. Photoreduction of the heme iron in the intact PSII membranes was completely suppressed by DCMU, whereas photoreduction and photooxidation of the heme iron in the Mn-depleted PSII membranes were unaffected by DCMU. Interestingly, photoreduction and photooxidation of the heme iron in the Mn-depleted PSII membranes were completely diminished by exogenous superoxide dismutase (SOD), whereas no effect of SOD on photoreduction of the heme iron was observed in the intact PSII membranes. The current work shows that the light-induced redox changes of cyt b(559) proceed via a different mechanism in the both types of PSII membranes. In the intact PSII membranes, photoreduction of the heme iron is mediated by plastoquinol. However, in the Mn-depleted PSII membranes, photoreduction and photooxidation of the heme iron are mediated by superoxide anion radical formed in PSII.
Sinha
Rakesh Kumar
RK
Department of Experimental Physics, Faculty of Science, Palacký University, Olomouc, Czech Republic.
Tiwari
Arjun
A
Pospísil
Pavel
P
D003573:Cytochrome b Group / Q000737:chemistry* / Q000378:metabolism*; D008345:Manganese / Q000172:deficiency / Q000378:metabolism*; D010084:Oxidation-Reduction; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D011487:Protein Conformation; D018724:Spinacia oleracea / Q000378:metabolism; D014867:Water / Q000378:metabolism*
D003573:Cytochrome b Group; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D014867:Water; D008345:Manganese; C019313:cytochrome b559