title: Structure-activity relationship of lupane-triterpene glycosides from Acanthopanax koreanum on spleen lymphocyte IL-2 and IFN-gamma.
abstract: Phytochemical investigation resulted in the isolation of three new lupane-triterpene glycosides acankoreosides M-O (1, 2 and 8) together with eight known lupane-triterpene glycosides (3-7, 9-11) from the leaves of Acanthopanax koreanum (Araliaceae). Their chemical structures were elucidated by mass, 1D and 2D NMR spectroscopy. The effect of eleven lupane-triterpene glycosides on Con A-induced splenolytic production of IL-2 and IFN-gamma were measured as markers of acquired immune responses. Compounds 4, 5, 7, and 11 (5, 25, and 100 microM) significantly increased IFN-gamma and IL-2 release in spleen cells.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Nguyen Huu
authors.initials: NH
authors.lastname: Tung
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Ha
authors.forename: Kwang Su
authors.initials: KS
authors.lastname: Soung
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D028001:Eleutherococcus / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry; D053978:Pentacyclic Triterpenes
chemical_list: D006027:Glycosides; D007371:Interferon-gamma; D007376:Interleukin-2; D012503:Saponins; D014315:Triterpenes; C553345:3,11,30-trihydroxylup-20(29)-en-23,28-dioic acid 28-O-(1-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl)ester; C553347:3,19-dihydroxylup-20(29)-en-23,28-dioic acid 28-O-(1-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl)ester; C553346:3-O-glucopyranosyl 3,11,30-trihydroxylup-20(29)-en-23,28-dioic acid 28-O-(1-rhamnopyranosyl-1-4-glucopyranosyl-1-6-glucopyranosyl)ester; C480546:lupane; D053978:Pentacyclic Triterpenes