title: Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244.
abstract: The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. alisa.v@chula.ac.th
authors.forename: A S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: W
authors.initials: W
authors.lastname: Promden
authors.forename: W
authors.initials: W
authors.lastname: De-Eknamkul
authors.forename: K
authors.initials: K
authors.lastname: Matsushita
authors.forename: H
authors.initials: H
authors.lastname: Toyama
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D020600:Gluconobacter oxydans / Q000145:classification / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012765:Shikimic Acid / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D012765:Shikimic Acid; D000429:Alcohol Oxidoreductases; C022511:quinate dehydrogenase