title: Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-γ and their efficacy against poultry coccidiosis.
abstract: The study describes vaccination experiments with highly immunogenic sporozoite E. acervulina cSZ-2 co-administered with chicken IL-2 (chIL-2) and interferon-γ (chIFN-γ) to determine their efficacies against homologue challenge. The entire coding sequence of cSZ2, chIL-2 and chIFN-γ were cloned into eukaryotic expression vector pVAX1, constructing DNA vaccines pVAX1-cSZ2, pVAX1-chIL-2, pVAX1-chIFN-γ, pVAX1-cSZ2-chIL-2 and pVAX1-cSZ2-chIFN-γ. The expression of target genes in vivo was detected by RT-PCR and Western blot. Chicken experiments were carried out by vaccinating chickens two times at dose rate of 100 μg intramuscularly. At 28 days of age, all chickens were inoculated orally with 1×10(5) sporulated oocysts of E. acervulina except the unchallenged control group. Seven days after challenge, all chickens were weighted and slaughtered for duodenum collection. The results indicated that these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The findings also demonstrated best synergistic effect of IL-2 with this protein which suggested that co-administration of cytokines with this antigen was a powerful method to enhance immunity by alleviating intestinal lesions, body weight loss and oocyst count imparting partial protection against homologous challenge.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Muhammad Ali A
authors.initials: MA
authors.lastname: Shah
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Song
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yan
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology / Q000378:metabolism*; D007371:Interferon-gamma / Q000737:chemistry / Q000378:metabolism*; D007376:Interleukin-2 / Q000737:chemistry / Q000378:metabolism; D033661:Oocysts; D011201:Poultry Diseases / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000276:immunology; D016052:Protozoan Vaccines / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA / Q000276:immunology*; D015430:Weight Gain
chemical_list: D007376:Interleukin-2; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma