title: [Generation of monoclonal antibody to GP73 based on antigen epitope].
abstract: OBJECTIVE
  Preparation of monoclonal antibody (mAb) against GP73 protein.
  
  
  METHODS
  The N-terminal peptide (AAAERGAVELK) of GP73 protein was displayed on T7 phage, the recombinant phage was amplified and used as the immunogen to immunize mouse to produce antibody. The titer of the antiserum and the positive hybridoma clones which secreted the mAb against GP73 protein were detected by ELISA. The mAb specificity was assayed by ELISA and Western blot.
  
  
  RESULTS
  The high specificity mAb against GP73 protein was selected from the mouse immunized with the recombinant T7 phages displaying the epitope of GP73 by cell fusion and screening.
  
  
  CONCLUSIONS
  The appropriate protein epitope displayed on T7 phage could be used as alternative antigen to immunize animals to make specific antibody against the corresponding native protein.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing 102206, China. xiningyao3579@yahoo.com.cn
authors.forename: Xi-ning
authors.initials: XN
authors.lastname: Yao
authors.forename: Jing-rui
authors.initials: JR
authors.lastname: Huo
authors.forename: Ying-fu
authors.initials: YF
authors.lastname: Liu
authors.forename: Bao-cai
authors.initials: BC
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Guo-cai
authors.initials: GC
authors.lastname: Fan
authors.forename: Qing-ming
authors.initials: QM
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; C410824:GOLM1 protein, human; D008565:Membrane Proteins