Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
The in vivo immunoadjuvant effects of CpG oligodeoxynucleotides (CpG-ODN) have been studied extensively in mice and relatively fewer studies have been done in piglets. But so far, the innate immunostimulatory effects of CpG-ODN combination with innate defense-regulator peptides (IDRs) have not been demonstrated. The purpose of this study is to determine the potential effects of CpG-ODN with IDR in newborn piglets. The immunostimulatory abilities of four selected IDRs were compared, among them HH2 showed best immunostimulatory effects in newborn piglets. Hereafter, the abilities of CpG-ODN combined with HH2 to enhance innate immune responses were examined in newborn piglets. The complex of HH2 and CpG-ODN could induce much stronger Th1 cytokine and chemokine responses than HH2 or CpG-ODN alone. HH2-CpG-ODN immunized piglets showed higher B cell percentage in PBMCs than CpG-ODN alone. These in vivo data demonstrated for the first time that subcutaneously (SC) administration of CpG-ODN combined with HH2 is efficient to stimulate innate immune system in newborn piglets.
Cao
Ding
D
College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
Li
Huazhou
H
Jiang
Zhenggu
Z
Xu
Chenchao
C
Cheng
Qing
Q
Yang
Zhaihan
Z
Cao
Guangjun
G
Zhang
Linghua
L
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000097:blood / Q000276:immunology; D007113:Immunity, Innate; D007114:Immunization; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology* / Q000494:pharmacology; D013552:Swine
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D016207:Cytokines; D009838:Oligodeoxyribonucleotides