Oxidative stress and inflammatory parameters after an Ironman race.
OBJECTIVE
The aim of the present study was to investigate oxidative stress markers and inflammatory response in triathletes after an Ironman race (IR).


METHODS
Descriptive research.


METHODS
Eighteen well-trained male triathletes (mean age, 34.7 +/- 2.15 years; weight, 69.3 +/- 1.9 kg; height, 1.81 +/- 0.58 cm) participated in the study.


METHODS
Ironman Triathlon (3.8-km swim, 180-km cycle, 42.2-km run). Mean environmental conditions ranged from 20 to 25 degrees C and from 79% to 85% relative humidity.


METHODS
None.


METHODS
Before the race and up to 20 minutes after completing the full race, the weights and heights of volunteers were measured and a 10 mL blood sample was drawn from an antecubital vein. Aliquots of washed/lysed red blood cells and plasma/serum samples were stored at -80 degrees C. Lipid peroxidation, protein carbonylation, superoxide dismutase and catalase activities, and cytokines levels [tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, IL-10, and IL-1ra] were determined.


RESULTS
After the IR, the results showed a significant increase in TBARS levels (prerace = 1.15 +/- 0.11; postrace = 1.98 +/- 0.27), lipid hydroperoxide content (prerace = 0.75 +/- 0.03; postrace = 1.46 +/- 0.18), protein carbonylation (prerace = 0.67 +/- 0.12; postrace = 2 0.15 +/- 0.60), superoxide dismutase (prerace = 2.67 +/- 0.62; postrace = 3.97 +/- 1.48), and catalase (prerace = 1.48 +/- 0.18; postrace = 2.84 +/- 0.39). TNF-alpha, IL-6, and IL-10 were not detected at basal conditions, but all markers were significantly increased after the IR (TNF-alpha: prerace = ND and postrace = 67.47 +/- 10.34; IL-6: prerace = ND and postrace = 55.41 +/- 3.45; IL-10: prerace = ND and postrace = 122.53 +/- 9.69; IL-1ra: prerace = 127.79 +/- 25.65 and postrace = 259.51 +/- 32.9).


CONCLUSIONS
An Ironman race provokes significant alterations in oxidative stress and inflammatory parameters. Thus, more studies with other markers and different designs are needed to elucidate the cellular alterations induced by an IR.
Pinho
Ricardo A
RA
Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil. pinho@unesc.net
Silva
Luciano A
LA
Pinho
Cleber A
CA
Scheffer
Débora L
DL
Souza
Cláudio T
CT
Benetti
Magnus
M
Carvalho
Tales
T
Dal-Pizzol
Felipe
F
D000328:Adult; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D016207:Cytokines / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D010807:Physical Endurance / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology; D050050:Protein Carbonylation; D013177:Sports / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D015415:Biomarkers; D016207:Cytokines; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase