title: Genotoxic evaluation of the insecticide endosulfan based on the induced GADD153-GFP reporter gene expression.
abstract: Endosulfan is one of the few organochlorine insecticides still in use in China for protecting crops from a variety of insects. Endosulfan is toxic in fishes and rodents in the in vivo assays, but its genotoxicity in mammalian cells has not been well tested. In this work, a genotoxic testing system has been developed based on the induction of a HepG2/GADD153-GFP reporter gene expression in response to the DNA-damaging agents. Methyl methanesulfonate, a known carcinogenic and genotoxic agent, was used to test the effects of damage dose and post-treatment incubation time on GADD153-GFP expression. Subsequently, the system was applied to the genotoxicity evaluation of endosulfan. Endosulfan was able to cause the increase of GADD153-GFP expression at a sublethal dose (0.02-20 mg/L). In particular, it induced a maximum green fluorescent protein expression at the tested concentration of 0.2 mg/L, with 4.07-fold inflorescence relative to untreated cells. The results suggest that endosulfan has the potential genotoxicity for HepG2 cell line by inducing DNA damage. The study also confirms that the induced GADD153-GFP expression system is an appropriate and sensitive method for the assessment of genotoxicity from a broad range of pesticides with the DNA-damaging potential.
authors.affiliation: College of Life Sciences, Graduate University of Chinese Academy of Sciences, Beijing, 100049, China. ldh_cas@yahoo.com.cn
authors.forename: Dahui
authors.initials: D
authors.lastname: Li
authors.forename: Jianzhang
authors.initials: J
authors.lastname: Liu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Li
mesh_terms: D004726:Endosulfan / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism
chemical_list: C497249:DDIT3 protein, human; D007306:Insecticides; D051742:Transcription Factor CHOP; D004726:Endosulfan