title: Expression of heat shock proteins in osteosarcomas.
abstract: OBJECTIVE
  Heat shock proteins (HSPs) protect cells against stress-associated injuries and are overexpressed in several malignant tumours. We investigated the potential roles of HSP27, HSP60, and HSP70 in conventional and low grade central osteosarcoma.
  
  
  METHODS
  Expressions of HSP27, HSP60, and HSP70 were analysed using immunohistochemistry on tissue sections from 52 cases of conventional osteosarcoma and 21 cases of low grade central osteosarcoma. We evaluated the expression of each protein and examined its relationship with clinicopathological parameters.
  
  
  RESULTS
  We found significantly different expressions of HSP27 and HSP70 between conventional and low grade central osteosarcoma [34.6% versus 4.8% (p = 0.008), 88.5% versus 14.3% (p < 0.001)]. However, HSP60 was highly expressed in both kinds of osteosarcoma (92.3% versus 85.7%). In conventional osteosarcoma, a higher expression of HSP27 was significantly related to distant metastasis (p = 0.034) and histological subtype [osteoblastic versus non-osteoblastic (p = 0.041)]. The expressions of HSP60 and HSP70 were not significantly related to any tested clinicopathological parameter.
  
  
  CONCLUSIONS
  HSP27 and HSP70 may be used as differential markers to distinguish conventional and low grade central osteosarcoma. HSP27 may be used as a possible prognostic marker in conventional osteosarcoma cases.
authors.affiliation: Department of Pathology, School of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Ahrim
authors.initials: A
authors.lastname: Moon
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bacchini
authors.forename: Franco
authors.initials: F
authors.lastname: Bertoni
authors.forename: Liliana G
authors.initials: LG
authors.lastname: Olvi
authors.forename: Eduardo
authors.initials: E
authors.lastname: Santini-Araujo
authors.forename: Youn Wha
authors.initials: YW
authors.lastname: Kim
authors.forename: Yong-Koo
authors.initials: YK
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism* / Q000401:mortality; D002648:Child; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D011379:Prognosis; D056910:Republic of Korea / Q000453:epidemiology; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D006360:Heat-Shock Proteins