[Extracts of danshen and chuanxinlian on human monocytic line THP-1 induced by TNF-alpha].
OBJECTIVE
To investigate whether extracts of Danshen and Chuanxinlian (SL) could promote the function recovery in pre-monocytic cell line THP-1 induced by TNF-alpha.


METHODS
SL extracts (0.125-2 g x L(-1)) were used to incubate THP-1 for 24 h before stimulation with TNF-alpha (20 microg x L(-1)), the adhesion, migration, lipid uptake and secretion of THP-1 were observed.


RESULTS
SL (0.5-2 g x L(-1)) had obvious effect on decreasing the THP-1 adhesion. The number of passed membrane was much fewer than that of control cells in SL (0.125-2 g x L(-1)). SL (0.125-2 g x L(-1)) reduced the total cholesterol content significantly. The levels of IL-6 in SL (2 g x L(-1)) were significantly decreased,and IL-10 was increased than that before the treatment.


CONCLUSIONS
SL extracts could promote the function recovery such as adhesion, migration, lipid uptake and secretion of THP-1 induced by TNF-alpha, which probably is one of the mechanisms of inhibit the inflammatory reaction in initiation and development of AS.
Li
Yujie
Y
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine Science, Beijing 100700, China.
Yang
Qing
Q
Weng
Xiaogang
X
Zhu
Xiaoxing
X
Wang
Yiwei
Y
Liu
Xiaoni
X
Han
Xiao
X
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha