An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells.
To reduce the interference in MTT reduction assay, we developed an improved protocol for evaluating the viability of Escherichia coli cells by conducting MTT reduction in 1.5-mL centrifuge tubes and formazan dissolution in test tubes. Our study is helpful in developing protocols for measuring the viability of other gram-negative bacteria.
Wang
Hengwei
H
Newworld Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China.
Cheng
Hairong
H
Wang
Fengqing
F
Wei
Dongzhi
D
Wang
Xuedong
X
D001431:Bacteriological Techniques / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism*
D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue