Single-allele analysis of transcription kinetics in living mammalian cells.
We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
Yunger
Sharon
S
The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.
Rosenfeld
Liat
L
Garini
Yuval
Y
Shav-Tal
Yaron
Y
D000483:Alleles; D019938:Cyclin D1 / Q000235:genetics; D004261:DNA Replication; D006801:Humans; D007700:Kinetics; D014158:Transcription, Genetic
D019938:Cyclin D1