Adiponectin is produced by lymphocytes and is a negative regulator of granulopoiesis.
Lymphocytes have long been established to play an important role in the regulation of hematopoiesis and produce many cytokines that act on hematopoietic progenitor cells. Previous studies by our group have shown that normal, unstimulated lymphocytes produce a protein that inhibits normal bone marrow GM colony formation. Adiponectin is an adipokine that has been demonstrated to act as a negative regulator of hematopoiesis and immune function. This study aimed to determine if the inhibitory molecule that we described previously was adiponectin. Here, we show transcription, translation, and secretion of adiponectin from lymphocytes and demonstrate that its receptors, AdipoR1 and AdipoR2, are expressed by bone marrow MNCs. We show that although the adiponectin expression is low in lymphocytes, it is sufficient to induce a significant inhibitory effect on GM precursors (CFU-GM) and activate the AMPK pathway in these cells. The regulation of adiponectin production by lymphocytes and its detailed function in suppressing GM colony formation need to be elucidated now. Our findings suggest a functional role for adiponectin as a negative regulator of granulopoiesis.
Crawford
Lisa J A
LJ
Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, Northern Ireland.
Peake
Roy
R
Price
Susan
S
Morris
Treen C M
TC
Irvine
Alexandra E
AE
D052242:Adiponectin / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D019891:Leukopoiesis / Q000502:physiology*; D008214:Lymphocytes / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D052242:Adiponectin; D054419:Receptors, Adiponectin