Effects of fibrous and non-fibrous dusts on Chinese hamster V79 cells: Cytotoxicity or cytostasis?
Chinese hamster V79 (CHV79) cells cultured in vitro were used to study the cytopathic effects of seven mineral dusts (four of which were fibrous). Lactate dehydrogenase release and the incorporation of [(3)H]thymidine into DNA were used to study toxic and static interactions, respectively. Crystalline silica dusts had a toxic effect on the cells. In contrast, Canadian chrysotile (chrysotile 'B') was relatively non-toxic but principally had a static effect upon CHV79 cells. Amphibole asbestos dusts demonstrated mainly static properties, albeit less marked than with chrysotile, and some toxic properties. Titanium dioxide was neither toxic nor static in vitro.
Lock
S O
SO
Environmental Safety Laboratory, Unilever Research & Engineering, Colworth House, Sharnbrook, Bedford MK44 1LQ, England.
Rockley
J
J
Chamberlain
M
M
Friend
J V
JV