title: Effects of UV-B radiation in morpho-genetic characters of Gnaphalium luteo-album.
abstract: Genetic analyses of Gnaphalium's DNA showed a high degree of polymorphism in UV-B irradiated plants when compared to controls. Among the five tested primers the four ISSR primers selected for this analysis generated a total of 189 fragments. A high proportion of polymorphic bands ranging from 70% to 28% were found using these ISSR markers. Nei and Li similarity indexes [1] were used to evaluate genetic divergence among plants. The dendrograms obtained using these markers efficiently separate plants from different treatments. A linear relationship was observed between UV-B dose and percentage of dissimilarity which may be related to DNA damage caused by the different UV-B treatments.
authors.affiliation: Universidad de Magallanes, Facultad de Ciencias, P.O. Box 113-D, Punta Arenas, Chile. pedro.cuadra@umag.cl
authors.forename: Pedro
authors.initials: P
authors.lastname: Cuadra
authors.forename: Daniela
authors.initials: D
authors.lastname: Vargas
authors.forename: Víctor
authors.initials: V
authors.lastname: Fajardo
authors.forename: Raúl
authors.initials: R
authors.lastname: Herrera
mesh_terms: D004249:DNA Damage; D018744:DNA, Plant / Q000032:analysis; D036622:Gnaphalium / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D014466:Ultraviolet Rays
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins