title: Detection of ciguatoxin-like and paralysing toxins in Trichodesmium spp. from New Caledonia lagoon.
abstract: Marine pelagic cyanobacteria Trichodesmium are widespread in the New Caledonia lagoon. Blooms of these Oscillatoriales are suspected to be a potential source of toxins in the ciguatera food chain and were previously reported to contain certain types of paralysing toxins. In the present study, toxicity experiments were conducted on lipid- and water-soluble extracts of freeze-dried samples of these cyanobacteria. Lipid-soluble fractions revealed a ciguatoxin-like activity in both in vivo (mouse bioassay) and in vitro (mouse neuroblastoma cells assay and receptor binding assay using tritiated brevetoxin-3) assays. The water-soluble fractions tested on mice exhibited neurotoxicity with paralytic symptoms. These toxicities have also been observed with benthic filamentous cyanobacteria within the Oscillatoriales order, also collected in New Caledonia. This study provides an unprecedented evidence of the toxicity of Trichodesmium species from the New Caledonia lagoon. This survey also demonstrates the possible role of these cyanobacteria in ciguatera fish poisoning.
authors.affiliation: Laboratoire Pharmacochimie des Substances Naturelles et Pharmacophores Redox, Université de Toulouse, UPS, UMR152, 118 Route de Narbonne, F-31062 Toulouse Cedex 9, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Kerbrat
authors.forename: H Taiana
authors.initials: HT
authors.lastname: Darius
authors.forename: Serge
authors.initials: S
authors.lastname: Pauillac
authors.forename: Mireille
authors.initials: M
authors.lastname: Chinain
authors.forename: Dominique
authors.initials: D
authors.lastname: Laurent
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D002922:Ciguatoxins / Q000302:isolation & purification / Q000633:toxicity*; D000458:Cyanobacteria / Q000737:chemistry*; D000087522:Cyanobacteria Toxins; D017753:Ecosystem; D005260:Female; D008297:Male; D008387:Marine Toxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D051379:Mice; D052998:Microcystins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D009498:Neurotoxins / Q000633:toxicity; D009510:New Caledonia; D018675:Toxicity Tests
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D008387:Marine Toxins; D052998:Microcystins; D009498:Neurotoxins; D002922:Ciguatoxins