title: MALDI-TOF MS combined with magnetic beads for detecting serum protein biomarkers and establishment of boosting decision tree model for diagnosis of hepatocellular carcinoma.
abstract: No single biologic marker is used in the routine diagnosis of hepatocellular carcinoma (HCC). We screened for potential biomarkers in 142 samples, including samples from 60 patients with HCC, 36 patients with hepatic disease other than HCC, and 46 age- and sex-matched healthy volunteers. Pretreated samples were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cationic exchange magnetic beads. The diagnostic pattern with a panel of 4 potential protein biomarkers of a mass-to-charge (m/z) ratio of 4,471, 8,936, 11,670, and 13,752 could accurately recognize 56 of 60 patients with HCC, 34 of 36 patients with hepatic disease, and 44 of 46 healthy people. The preliminary data suggest a potential application of MALDI-TOF MS combined with magnetic beads as an effective technology to profile serum proteome, and, with pattern analysis, a diagnostic model comprising 4 potential biomarkers was indicated to differentiate people with hepatic disease and healthy control subjects rapidly and precisely.
authors.affiliation: Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, China.
authors.forename: Chibo
authors.initials: C
authors.lastname: Liu
authors.forename: Jianmin
authors.initials: J
authors.lastname: Shen
authors.forename: Chunqin
authors.initials: C
authors.lastname: Pan
authors.forename: Linjun
authors.initials: L
authors.lastname: Yang
authors.forename: Sihua
authors.initials: S
authors.lastname: Mou
authors.forename: Haibao
authors.initials: H
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis*; D003663:Decision Trees; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D001798:Blood Proteins