Transforming growth factor beta1-induced heat shock protein 27 activation promotes migration of mouse dental papilla-derived MDPC-23 cells.
BACKGROUND
Transforming growth factor beta1 (TGFbeta1) regulates cellular functions including cell growth, differentiation, angiogenesis, migration, and metastasis. The TGFbeta1 signal transduction pathways are mostly undefined in mouse dental papilla-derived MDPC-23 cells. In this study, we investigated TGFbeta1-induced migration focusing on heat shock protein 27 (Hsp27) activation.


METHODS
Cellular responses mediated by TGFbeta1 in MDPC-23 cells were measured by Western blot and MTT assays. Cell migration was determined by counting migrated cells using the chemotaxis cell migration assay.


RESULTS
TGFbeta1 induced cell migration and increased the phosphorylation of Hsp27 and p38 MAPK in MDPC-23 cells. However, TGFbeta1 did not affect Akt/NF-kappaB signaling to regulate the migration of MDPC-23 cells. Inhibiting p38 MAPK with SB203580 blocked TGFbeta1-induced Hsp27 activation and cell migration.


CONCLUSIONS
Hsp27 phosphorylation followed by p38 MAPK activation was required for TGFbeta1-induced migration, and Hsp27 itself contributed to MDPC-23 cell migration.
Kwon
Seong-Min
SM
Department of Pathology, School of Dentistry Chosun University, Gwangju 501-759, Korea.
Kim
Soo-A
SA
Yoon
Jung-Hoon
JH
Ahn
Sang-Gun
SG
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D004396:Coloring Agents; D003771:Dental Papilla / Q000166:cytology / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D055551:HSP27 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000187:drug effects
D004396:Coloring Agents; D004791:Enzyme Inhibitors; D055551:HSP27 Heat-Shock Proteins; C486699:Hspb2 protein, mouse; D007093:Imidazoles; D016328:NF-kappa B; D011725:Pyridines; D013778:Tetrazolium Salts; D013844:Thiazoles; D053773:Transforming Growth Factor beta1; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; C022616:thiazolyl blue; C093642:SB 203580