title: Evaluation of Zinc-α-2-Glycoprotein and Proteasome Subunit β-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology.
abstract: Prostate cancer (CaP) is a significant cause of illness and death in males. Current detection strategies do not reliably detect the disease at an early stage and cannot distinguish aggressive versus nonaggressive CaP leading to potential overtreatment of the disease and associated morbidity. Zinc-α-2-glycoprotein (ZAG) and proteasome subunit β-Type 6 (PSMB-6) were found to be up-regulated in the serum of CaP patients with higher grade tumors after 2-dimensional difference gel electrophoresis analysis. The aim of this study was to investigate if ZAG and PSMB-6 were also overexpressed in prostatic tumor tissue of CaP patients. Immunohistochemical analysis was performed on CaP tissue microarrays with samples from 199 patients. Confirmatory gene expression profiling for ZAG and PSMB-6 were performed on 4 cases using Laser Capture Microdissection and TaqMan real-time polymerase chain reaction. ZAG expression in CaP epithelial cells was inversely associated with Gleason grade (benign prostatic hyperplasia>G3>G4/G5). PSMB-6 was not expressed in either tumor or benign epithelium. However, strong PSMB-6 expression was noted in stromal and inflammatory cells. Our results indicate ZAG as a possible predictive marker of Gleason grade. The inverse association between grade and tissue expression with a rising serum protein level is similar to that seen with prostate-specific antigen. In addition, the results for both ZAG and PSMB-6 highlight the challenges in trying to associate the protein levels in serum with tissue expression.
authors.affiliation: Department of Pathology, RCSI ERC Beaumont Hospital. gillianohurley@rcsi.ie
authors.forename: Gillian
authors.initials: G
authors.lastname: O'Hurley
authors.forename: Anthony
authors.initials: A
authors.lastname: O'Grady
authors.forename: Paul
authors.initials: P
authors.lastname: Smyth
authors.forename: Jennifer
authors.initials: J
authors.lastname: Byrne
authors.forename: John J
authors.initials: JJ
authors.lastname: O'Leary
authors.forename: Orla
authors.initials: O
authors.lastname: Sheils
authors.forename: R
authors.initials: R
authors.lastname: William
authors.forename: G
authors.initials: G
authors.lastname: Watson
authors.forename: Elaine W
authors.initials: EW
authors.lastname: Kay
mesh_terms: D000328:Adult; D000368:Aged; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D046888:Tissue Array Analysis; D000090203:Zn-Alpha-2-Glycoprotein
chemical_list: D029589:Seminal Plasma Proteins; D000090203:Zn-Alpha-2-Glycoprotein; D046988:Proteasome Endopeptidase Complex