Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer.
BACKGROUND
The melanoma-associated antigen (MAGE) gene family consists of different expression patterns in various tumor types. They are considered tumor-specific antigens and are ideal targets for cancer immunotherapy. The purpose of this study is to identify the expression profiles of the MAGE family genes in Taiwanese colorectal cancer patients.


METHODS
In this study, a well-constructed chip array platform was used to analyze the expression of the MAGE family genes of 100 colorectal cancer tissues. Statistical analysis of the experimental results and patients' clinical manifestations were also conducted.


RESULTS
The results showed MAGE-A2 (87%), -A7 (83%), -A8 (75%), -A12 (71%), -B2 (75%), -B3 (79%), -D2 (75%), -F1 (79%), and -H1 (70%) were significantly overexpressed genes in colorectal cancer tissues. MAGE-A2 was the most highly overexpressed gene among the MAGE family. MAGE-B3 gene expression is statistically correlated with tumor size, lymph node, and UICC stage. In addition, the overexpression of MAGE-D2 and -H1 genes are statistically correlated to the tumor size and depth, respectively (P < 0.05).


CONCLUSIONS
This is the first comprehensive report to clarify the differential expression profile of whole MAGE family in CRCs, and it might provide some crucial information about the carcinogenesis and progression in Taiwanese patients with CRC.
Chung
Fu-Yen
FY
Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Cheng
Tian-Lu
TL
Chang
Hui-Jen
HJ
Chiu
Hua-Hsien
HH
Huang
Ming-Yii
MY
Chang
Ming-Sung
MS
Chen
Chung-Chi
CC
Yang
Ming-Je
MJ
Wang
Jaw-Yuan
JY
Lin
Shiu-Ru
SR
D000951:Antigens, Neoplasm / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D013624:Taiwan
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins