Electrochemical investigations of cytochrome P450.
In this paper we summarized our experimental data on the electrochemical reduction of cytochrome P450. Electrode/cytochrome P450 systems were analyzed in terms of the mechanisms underlying P450-catalyzed reactions. Bioelectrocatalysis-based screening of potential substrates or inhibitors of cytochrome P450, stoichiometry of the electrocatalytic cycle, redox thermodynamics and the peroxide shunt pathway were described. Characteristics, performance and potential application of cytochrome P450-electrodes are discussed.
Shumyantseva
Victoria V
VV
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10, Moscow 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
Bulko
Tatiana V
TV
Suprun
Elena V
EV
Chalenko
Yaroslava M
YM
Vagin
Michail Yu
MY
Rudakov
Yurii O
YO
Shatskaya
Marina A
MA
Archakov
Alexander I
AI
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D055162:Biocatalysis / Q000187:drug effects; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D000072467:Cytochrome P450 Family 2; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008797:Metyrapone / Q000494:pharmacology; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects; D011817:Rabbits; D013379:Substrate Specificity; D013816:Thermodynamics
D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit); D008797:Metyrapone