Rhodamine-based chemosensor for Hg(2+) in aqueous solution with a broad pH range and its application in live cell imaging.
A new fluorescent probe, rhodamine B derivative (1) bearing an 8-hydroxyquinoline group, was synthesized and displayed highly selective and sensitive Hg(2+)-amplified absorbance and fluorescence emission above 500 nm in aqueous solution with a broad pH range 4-9. It was found that mercury ions coordinate reversibly to 1 and the spirolactam ring of 1 was opened, forming a 1 : 1 metal-ligand complex. Furthermore, this sensor was applied for in vivo imaging in HeLa cells to confirm that 1 can be used as a fluorescent probe for monitoring Hg(2+) in living cells.
Zhao
Yun
Y
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Sun
Yue
Y
Lv
Xin
X
Liu
Yunlong
Y
Chen
Maliang
M
Guo
Wei
W
D002470:Cell Survival; D002478:Cells, Cultured; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008628:Mercury / Q000032:analysis*; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D015125:Oxyquinoline / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D012996:Solutions; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D008024:Ligands; D012235:Rhodamines; D012996:Solutions; D014867:Water; D015125:Oxyquinoline; D008628:Mercury; C029773:rhodamine B