Effect of ompR gene mutation in expression of ompC and ompF of Salmonella typhi.
In the present investigation, a total of 50 stool samples were collected from the food handlers to screen the typhoid asymptomatic carriers, positive result was yielded for 2 out of the 50 samples. Salmonella typhi was isolated and identified based on the cultural characteristics on BSA, Macconkey agar, XLD and phylogenetic analysis. The ompR region of these two strains was amplified, sequenced and a Neighbor-Joining algorithm tree of ompR was constructed. The isolates were designated as (Salmonella Strain) SS-3 and SS-5 respectively. The isolates were subjected to mutation using sodium chloride at various osmolarity concentrations in LB broth. Both the wild and mutant Salmonella typhi were used for the isolation of outer membrane protein. The outer membrane protein was isolated and compared with both the wild and mutated Salmonella typhi. The expression of outer membrane protein was showing the variation in the expression which were noticed by using SDS-PAGE. On the basis of the results, it was concluded that the ompR-envZ two component regulatory systems play an important role on the regulation of Vi polysaccharide synthesis in S. typhi, and that one of the environmental signals for this regulation may be osmolarity.
Malickbasha
Mahaboobbasha
M
Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, TN, India.
Arunachalam
Ramaiah
R
Senthilkumar
Balakrishnan
B
Rajasekarapandian
Moses
M
Annadurai
Gurusamy
G
D000465:Algorithms; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005786:Gene Expression Regulation; D008957:Models, Genetic; D009154:Mutation; D010802:Phylogeny; D011134:Polysaccharides / Q000737:chemistry; D018272:Porins / Q000737:chemistry / Q000235:genetics*; D012485:Salmonella typhi / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics*; D014510:Urease / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C113724:OmpC protein; C109237:OmpF protein; D011134:Polysaccharides; D018272:Porins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; D012965:Sodium Chloride; D014510:Urease