Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles.
The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a significant and concomitant increase in the percent of intermediate and primary follicles in all treatments tested after 7 days of culture when compared to non-cultured tissue. After 7 days of culture, the highest percent of intermediate follicles was observed with IGF-I/GH (61.3%), and the highest percent of primary follicles was achieved with IGF-I (57.7%). After 7 days of culture in MEM+ containing GDF-9, IGF-I and GH alone or in all associations, a significant increase in follicular diameter was observed when compared to MEM+ alone and non-cultured tissue. In conclusion, GDF-9, IGF-I and GH alone or in combination maintain preantral follicle survival and promote primordial follicle activation. Nevertheless, the data showed that IGF-I/GH and IGF-I alone are efficient in promoting the transition from primordial to intermediate follicles and from intermediate to primary follicles, respectively.
Martins
F S
FS
Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais, Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, CE, Brasil. fabricio@netbandalarga.com.br
Celestino
J J H
JJ
Saraiva
M V A
MV
Chaves
R N
RN
Rossetto
R
R
Silva
C M G
CM
Lima-Verde
I B
IB
Lopes
C A P
CA
Campello
C C
CC
Figueiredo
J R
JR
D000818:Animals; D049109:Cell Proliferation; D005260:Female; D006041:Goats; D055429:Growth Differentiation Factor 9 / Q000494:pharmacology*; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D046509:Tissue Culture Techniques
D055429:Growth Differentiation Factor 9; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone