[Down-regulation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway].
OBJECTIVE
To investigate the changes in SiHa cell apoptosis after inhibition of CD147 expression.


METHODS
RNA interference (RNAi) technique was used to down-regulate CD147 expression in SiHa cells, and RT-PCR and Western blotting were used to detect expression of CD147, Bcl-2, Bim and caspase-3; the percentage of cell apoptosis were detected by flow cytometry.


RESULTS
SiRNA sequence 1, 2 inhibited CD147 expression in SiHa cells effectively (P<0.05), resulting also in down-regulated expression of Bcl-2 (P<0.05) and up-regulated expression of caspase-3 and Bim(P<0.05). The percentage of apoptotic cells increased significantly, and early apoptosis was the most obvious in the cells (P<0.05).


CONCLUSIONS
Silencing of CD147 expression induces SiHa cell apoptosis partially through the Bcl-2 pathway .
Zhou
Xi
X
Department of Obstetrics and Gynecology, People's Hospital Affiliated to Yunyang Medical College, Shiyan 442000, China. xzho98@yahoo.com.cn
Lv
Jie-yu
JY
Gong
Bao-lan
BL
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D051926:Basigin / Q000378:metabolism*; D000072224:Bcl-2-Like Protein 11; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D034622:RNA Interference
D051017:Apoptosis Regulatory Proteins; C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051926:Basigin; C505407:CASP3 protein, human; D053148:Caspase 3