title: The polyphenolics in the aqueous extract of Psidium guajava kinetically reveal an inhibition model on LDL glycation.
abstract: Guava [Psidium guajava L. (Myrtaceae)] budding leaf extract (PE) has shown tremendous bioactivities. Previously, we found seven major compounds in PE, i.e., gallic acid, catechin, epicatechin, rutin, quercetin, naringenin, and kaempferol. PE showed a potentially active antiglycative effect in an LDL (low density lipoprotein) mimic biomodel, which can be attributed to its large content of polyphenolics. The glycation and antiglycative reactions showed characteristic distinct four-phase kinetic patterns. In the presence of PE, the kinetic coefficients were 0.000438, 0.000060, 0.000, and -0.0001354 ABS-mL/mg-min, respectively, for phases 1 to 4. Computer simulation evidenced the dose-dependent inhibition model. Conclusively, PE contains a large amount of polyphenolics, whose antiglycative bioactivity fits the inhibition model.
authors.affiliation: Department of Urology, Taipei Medical University Shuang-Ho Hospital, Taipei, Taiwan.
authors.forename: Kuan-Chou
authors.initials: KC
authors.lastname: Chen
authors.forename: Chao-Ming
authors.initials: CM
authors.lastname: Chuang
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Lin
authors.forename: Wen-Ta
authors.initials: WT
authors.lastname: Chiu
authors.forename: Hui-Er
authors.initials: HE
authors.lastname: Wang
authors.forename: Chiu-Lan
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Tsuimin
authors.initials: T
authors.lastname: Tsai
authors.forename: Robert Y
authors.initials: RY
authors.lastname: Peng
mesh_terms: D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000493:pharmacokinetics*; D017127:Glycation End Products, Advanced; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008954:Models, Biological; D010636:Phenols / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000302:isolation & purification / Q000493:pharmacokinetics*; D018515:Plant Leaves; D059808:Polyphenols; D031647:Psidium; D014867:Water / Q000378:metabolism*; D000093844:Glycated Proteins
chemical_list: D005419:Flavonoids; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins