title: Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles.
abstract: The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a significant and concomitant increase in the percent of intermediate and primary follicles in all treatments tested after 7 days of culture when compared to non-cultured tissue. After 7 days of culture, the highest percent of intermediate follicles was observed with IGF-I/GH (61.3%), and the highest percent of primary follicles was achieved with IGF-I (57.7%). After 7 days of culture in MEM+ containing GDF-9, IGF-I and GH alone or in all associations, a significant increase in follicular diameter was observed when compared to MEM+ alone and non-cultured tissue. In conclusion, GDF-9, IGF-I and GH alone or in combination maintain preantral follicle survival and promote primordial follicle activation. Nevertheless, the data showed that IGF-I/GH and IGF-I alone are efficient in promoting the transition from primordial to intermediate follicles and from intermediate to primary follicles, respectively.
authors.affiliation: Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais, Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, CE, Brasil. fabricio@netbandalarga.com.br
authors.forename: F S
authors.initials: FS
authors.lastname: Martins
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.forename: R
authors.initials: R
authors.lastname: Rossetto
authors.forename: C M G
authors.initials: CM
authors.lastname: Silva
authors.forename: I B
authors.initials: IB
authors.lastname: Lima-Verde
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D005260:Female; D006041:Goats; D055429:Growth Differentiation Factor 9 / Q000494:pharmacology*; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D046509:Tissue Culture Techniques
chemical_list: D055429:Growth Differentiation Factor 9; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone