Temporal and spatial expression of Muc1 during implantation in sows.
Recent evidence points to an important role for Muc1 in embryo implantation. In this study, Real-time PCR and immunohistochemistry were used to study mRNA and protein levels at, and between, the attachment sites of the endometrium of Day 13, 18 and 24 pregnant sows. The results indicate that Muc1 mRNA expression was higher between attachment sites than at attachment sites during implantation and this effect was significant on Day 13 (P < 0.01) and 24 (P < 0.01). Intense Muc1 immunostaining was observed in luminal epithelium and stroma and the staining between attachment sites was stronger than at attachment sites on Days 13 and 18. Collectively, these results suggest the crucial role of Muc1 in successful implantation and embryo survival.
Ren
Qian
Q
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; E-Mails: qianer0101@gmail.com (Q.R.); guanshu8@gmail.com (S.G.).
Guan
Shu
S
Fu
Jinluan
J
Wang
Aiguo
A
D000818:Animals; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D018396:Mucin-1 / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013997:Time Factors
D018396:Mucin-1