title: Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the blaVIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae.
abstract: The nucleotide sequence of pNL194, a VIM-1-encoding plasmid, is described in this study. pNL194 (79,307 bp) comprised an IncN-characteristic segment (38,940 bp) and a mosaic structure (40,367 bp) including bla(VIM-1), aacA7, aadA1, aadA2, dfrA1, dfrA12, aphA1, strA, strB, and sul1. Tn1000 or Tn5501 insertion within fipA probably facilitated recruitment of additional mobile elements carrying resistance genes.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens 11521, Greece. miriagou@pasteur.gr
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.forename: C C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.forename: S D
authors.initials: SD
authors.lastname: Kotsakis
authors.forename: A
authors.initials: A
authors.lastname: Loli
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: N J
authors.initials: NJ
authors.lastname: Legakis
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases