Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B.
The producers of glycolipid biosurfactant, mannosylerythritol lipid-B (MEL-B), were isolated from leaves of Perilla frutescens on Ibaraki in Japan. Four isolates, 1D9, 1D10, 1D11, and 1E5, were identified as basidiomycetous yeast Pseudozyma tsukubaensis by rDNA sequence and biochemical properties. The structure of MEL-B produced by these strains was analyzed by (1)H nuclear magnetic resonance and gas chromatography-mass spectrometry methods, and was determined to be the same as the diastereomer MEL-B produced by P. tsukubaensis NBRC 1940. Of these isolates, P. tsukubaensis 1E5 (JCM 16987) is capable of producing the largest amount of the diastereomer MEL-B from vegetable oils. In order to progress the diastereomer MEL-B production by strain 1E5, factors affecting the production, such as carbon and organic nutrient sources, were further examined. Olive oil and yeast extract were the best carbon and nutrient sources, respectively. Under the optimal conditions, a maximum yield, productivity, and yield coefficient of 73.1 g/L, 10.4 g L(-1) day(-1), and 43.5 g/g were achieved by feeding of olive oil in a 5-L jar-fermenter culture using strain 1E5.
Morita
Tomotake
T
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1, Tsukuba, Ibaraki 305-8565, Japan.
Takashima
Masako
M
Fukuoka
Tokuma
T
Konishi
Masaaki
M
Imura
Tomohiro
T
Kitamoto
Dai
D
D001487:Basidiomycota / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D006017:Glycolipids / Q000096:biosynthesis*; D007218:Industrial Microbiology; D009682:Magnetic Resonance Spectroscopy; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D013501:Surface-Active Agents / Q000378:metabolism*; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D006017:Glycolipids; D012331:RNA, Fungal; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid