A safe and practical procedure for global deprotection of oligoribonucleotides.
We report a practical global deprotection of RNA 2'-O-tert-butyldimethylsilyl (TBS) ethers using commercially available aqueous NH(4)F. The procedure is applicable to both 96-well plate format and large-scale production of RNA. This improved procedure provides a safe, mild, and cost-effective alternative to highly hazardous Et(3)N x 3 HF, a reagent commonly used in the routine synthesis of RNA.
Zewge
Daniel
D
Department of Process Research, Merck Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065, USA. daniel_zewge@merck.com
Gosselin
Francis
F
Sidler
Rick
R
DiMichele
Lisa
L
Cvetovich
Raymond J
RJ
D064751:Ammonium Compounds; D002851:Chromatography, High Pressure Liquid; D005459:Fluorides / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D064751:Ammonium Compounds; D009843:Oligoribonucleotides; D000644:Quaternary Ammonium Compounds; C024822:ammonium fluoride; D005459:Fluorides