Crystallography of membrane proteins: from crystallization to structure.
Although crystallographic studies of membrane proteins have progressed in the last 5 years, the field still remains challenging with several severe bottlenecks. The chapter focuses on the crystallization and describes two approaches, the classical vapor diffusion method and the more recent use of lipidic phases. General aspects on the crystallization principles as well as more practical details are given. In a more synthetic way, the chapter also addresses how structures are solved by X-ray crystallography, and highlights aspects that are specific to membrane proteins.
Deniaud
Aurélien
A
European Molecular Biology Laboratory, Grenoble Outstation, B.P. 181, Grenoble, France.
Moiseeva
Ekaterina
E
Gordeliy
Valentin
V
Pebay-Peyroula
Eva
E
D018360:Crystallography, X-Ray / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008962:Models, Theoretical; D017433:Protein Structure, Secondary
D008565:Membrane Proteins