title: miR-29b and miR-125a regulate podoplanin and suppress invasion in glioblastoma.
abstract: Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation and invasion. Recently, it was demonstrated that podoplanin membrane sialo-glycoprotein encoded by PDPN gene is over-expressed and related to cellular invasion in astrocytic tumors; however the mechanisms of regulation are still unknown. MicroRNAs are noncoding RNAs that regulate gene expression and several biological processes and diseases, including cancer. Nevertheless, their roles in invasion, proliferation, and apoptosis of glioblastoma are not completely understood. In this study, we focused on miR-29b and miR-125a, which were predicted to regulate PDPN, and demonstrated that these microRNAs directly target the 3' untranslated region of PDPN and inhibit invasion, apoptosis, and proliferation of glioblastomas. Furthermore, we report that miR-29b and miR-125a are downregulated in glioblastomas and also in CD133-positive cells. Taken together, these results suggest that miR-29b and miR-125a represent potential therapeutic targets in glioblastoma.
authors.affiliation: Department of Experimental Therapeutics and Center for RNA Interference and Non-coding RNAs, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: Maria Angelica
authors.initials: MA
authors.lastname: Cortez
authors.forename: Milena Sabrina
authors.initials: MS
authors.lastname: Nicoloso
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Simona
authors.initials: S
authors.lastname: Rossi
authors.forename: Gopal
authors.initials: G
authors.lastname: Gopisetty
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Molina
authors.forename: Carlos
authors.initials: C
authors.lastname: Carlotti
authors.forename: Daniela
authors.initials: D
authors.lastname: Tirapelli
authors.forename: Luciano
authors.initials: L
authors.lastname: Neder
authors.forename: Maria Sol
authors.initials: MS
authors.lastname: Brassesco
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Scrideli
authors.forename: Luiz Gonzaga
authors.initials: LG
authors.lastname: Tone
authors.forename: Maria-Magdalena
authors.initials: MM
authors.lastname: Georgescu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Vinay
authors.initials: V
authors.lastname: Puduvalli
authors.forename: George Adrian
authors.initials: GA
authors.lastname: Calin
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000502:physiology*; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D035683:MicroRNAs; C117468:PDPN protein, human; D000098853:Podoplanin