miR-1/miR-206 regulate Hsp60 expression contributing to glucose-mediated apoptosis in cardiomyocytes.
Hsp60 is an important component of defense mechanisms against diabetic myocardial injury; however, the cause of Hsp60 reduction in the diabetic myocardium remains unknown. After stimulation of cardiomyocytes with high glucose in vivo and in vitro, significant up-regulation of miR-1/miR-206 and post-transcriptional modulation of Hsp 60 were observed. Serum response factor (SRF) and the MEK1/2 pathway were involved in miR-1 and miR-206 expression in cardiomyocytes. miR-1 and miR-206 regulated Hsp60 expression post-transcriptionally and accelerated cardiomyocyte apoptosis through Hsp60. These results revealed that miR-1 and miR-206 regulate Hsp60 expression, contributing to high glucose-mediated apoptosis in cardiomyocytes.
Shan
Zhi-Xin
ZX
Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
Lin
Qiu-Xiong
QX
Deng
Chun-Yu
CY
Zhu
Jie-Ning
JN
Mai
Li-Ping
LP
Liu
Ju-Li
JL
Fu
Yong-Heng
YH
Liu
Xiao-Ying
XY
Li
Yang-Xin
YX
Zhang
You-Yi
YY
Lin
Shu-Guang
SG
Yu
Xi-Yong
XY
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005947:Glucose / Q000494:pharmacology*; D020935:MAP Kinase Signaling System; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D008955:Models, Cardiovascular; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D051381:Rats; D017207:Rats, Sprague-Dawley; D026362:Serum Response Factor / Q000378:metabolism
D018834:Chaperonin 60; C542191:MIRN1 microRNA, rat; D035683:MicroRNAs; D026362:Serum Response Factor; C522220:mirn206 microRNA, rat; D004247:DNA; D005947:Glucose