[Biological characters of a coxsackievirus B3 variant strain isolated from a hand-foot-mouth disease patient with severe clinical symptoms].
OBJECTIVE
To analyze the genetic and biological characters of a new isolate of coxsackievirus B3 (CoxB3), i.e. FY-19 strain, and investigate its mechanistic role in causing different clinical symptoms of hand-foot-mouth disease (HFMD).


METHODS
FY-19 strain, isolated from a patient with severe clinical symptoms from Fuyang, China in 2008, was identified by the serological parameters via the Lim Benyesh-Melnick (LBM) antiserum pools. Its genotype was further characterized by sequencing the whole genome. And its biological characters were also examined by proliferation kinetic and pathogenetic analysis.


RESULTS
FY-19 strain was identified as CoxB3 showing 23.0%, 16.5% and 32.1% difference with Nancy strain in 3'-, 5'-noncoding and coding regions respectively. FY-19 also showed a high homology with other HFMD-related CoxB3 isolates in China. But its homology with non-HFMD-related CoxB3 isolates was lower (13.5% and 25.0% difference in 3'-NCR and coding region respectively). The viral replication kinetic analysis suggested that the FY-19 proliferation increased rapidly and peaked at 14 hours post-infection. In pathological analysis, FY-19 strain induced mortal pathology in sucking mice.


CONCLUSIONS
Differences in genetic and biological characters exist between FY-19 and Nancy strains. Further analysis on the pathogenesis of this variant may aid in elucidating the mechanisms of HFMD.
Shi
Hai-jing
HJ
Department of Virus Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
Liu
Jian-sheng
JS
Wang
Li-chun
LC
Zhang
Xue-mei
XM
Liu
Long-ding
LD
Liao
Yun
Y
Na
Rui-xiong
RX
Li
Qi-han
QH
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D051379:Mice; D012367:RNA, Viral; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins