Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles.
On the basis of copper-enhanced gold nanoparticle tags as an amplification approach, we introduced, in this paper, magnetic nanoparticles for further improving performance of electrochemical immunoassay by anodic stripping voltammetry (ASV) at a glassy-carbon electrode. Due to the use of antibody-immobilized magnetic nanoparticles, the immunoreaction between antibody and antigen takes place in a homogeneous bulk solution phase. Compared with traditional solid interface reaction, the proposed strategy can provide some advantages such as easy of separation, shorter analytical time, wider linear range, and lower detection limit. It was also successfully applied to HBsAg determination in a linear range of 0.1-1500 ng mL(-1) with a detection limit of 87 pg mL(-1). The proposed analytical strategy holds good selectivity, sensitivity and repeatability and also great promise for the extended application in the fields of clinical diagnosis, bio-affinity assay and environmental monitoring.
Shen
Guangyu
G
College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, 138 Dong-Ting Road, Changde 415000, Hunan, China. sgyrab@yahoo.com.cn
Zhang
Yun
Y
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002244:Carbon / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D013499:Surface Properties
D000906:Antibodies; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D002244:Carbon; D006046:Gold; D003300:Copper