MALDI-TOF MS analysis of condensed tannins with potent antioxidant activity from the leaf, stem bark and root bark of Acacia confusa.
The structures of the condensed tannins from leaf, stem bark and root bark of Acacia confusa were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and their antioxidant activities were measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing/antioxidant power (FRAP) assays. The results showed that the condensed tannins from stem bark and root bark include propelargonidin and procyanidin, and the leaf condensed tannins include propelargonidin, procyanidin and prodelphinidin, all with the procyanidin dominating. The condensed tannins had different polymer chain lengths, varying from trimers to undecamers for leaf and root bark and to dodecamers for stem bark. The condensed tannins extracted from the leaf, stem bark and root bark all showed a very good DPPH radical scavenging activity and ferric reducing power.
Wei
Shu-Dong
SD
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
Zhou
Hai-Chao
HC
Lin
Yi-Ming
YM
Liao
Meng-Meng
MM
Chai
Wei-Ming
WM
D000045:Acacia / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013634:Tannins / Q000737:chemistry*
D000975:Antioxidants; D013634:Tannins