[Significance of IL-1beta-induced ectopic expression of CDX2 in the intestinal metaplasia of gastric epithelium].
OBJECTIVE
To evaluate the effect of IL-1beta on the expression of CDX2 in human gastric epithelial cell line GES-1 and its role in the intestinal metaplasia.


METHODS
GES-1 cells were treated with IL-1beta in different concentrations and the expressions of CDX2 mRNA and protein were detected by real-time PCR, immunocytochemistry and Western blot at different time points. GES-1 cells were then pre-treated with NF-KappaB pathway inhibitor PDTC, and the expression of CDX2 mRNA and protein induced by IL-1beta were detected. The cell ultra-structure of GES-1 cells was observed by electronic microscope after GES-1 being treated with IL-1beta for 25 days.


RESULTS
Levels of CDX2 mRNA and protein were 0.0749 + or - 0.0021 and 0.56 + or - 0.04 in the cells treated with 1 microg/L IL-1beta(P<0.05). After pre-treatment with PDTC, levels of CDX2 mRNA and protein were 0.0006 + or - 0.0002 and 0.40 + or - 0.06(P<0.05). Some changes in the cell ultra-structure of GES-1 were found by electronic microscope when GES-1 was treated with IL-1beta for 25 days.


CONCLUSIONS
IL-1beta can stimulate CDX2 mRNA and protein expression in GES-1 cells through the NF-KappaB signal pathway, indicating that IL-1beta plays an important role in the intestinal metaplasia.
Li
Jiang
J
Department of Laparoscopic Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China.
Wang
Guo-bin
GB
Sun
Ren-hu
RH
Tao
Kai-xiong
KX
D000071616:CDX2 Transcription Factor; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism* / Q000473:pathology; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D008679:Metaplasia; D012333:RNA, Messenger / Q000235:genetics
D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; D018398:Homeodomain Proteins; D053583:Interleukin-1beta; D012333:RNA, Messenger