title: Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system.
abstract: We have developed a filter-chip and optical detection system for rapid antibiotic efficacy screening. The filter-chip consisted of a 1-mL reservoir and an anodic aluminum oxide (AAO) nanoporous membrane. Sample solution with liquid growth media, bacteria, and antibiotics was incubated in the reservoir for a specific period of time. The number of live bacteria on the surface of membrane was counted after the incubation with antibiotics and filtration. Using this biosensing system, we have demonstrated a 1-h antibiotic screening for patients' clinical samples, significantly faster than the conventional antibiotic susceptibility tests that typically take more than 24h. This rapid screening nature makes the filter-chip and detection system ideal for tailoring antibiotic treatment to individual patients by reducing the microbial antibiotic resistance, and improving the survival rate for patients suffering from postoperative infections.
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, Zachry Engineering Center, College Station, TX 77843, USA.
authors.forename: Pei-Hsiang
authors.initials: PH
authors.lastname: Tsou
authors.forename: Harini
authors.initials: H
authors.lastname: Sreenivasappa
authors.forename: Sungmin
authors.initials: S
authors.lastname: Hong
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yasuike
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Keiyo
authors.initials: K
authors.lastname: Nakano
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Misawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kameoka
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D055096:Optical Devices; D014462:Ultrafiltration / Q000295:instrumentation*
chemical_list: D000900:Anti-Bacterial Agents; D000537:Aluminum Oxide