title: Bacillus anthracis spore entry into epithelial cells is an actin-dependent process requiring c-Src and PI3K.
abstract: Dissemination of Bacillus anthracis from the respiratory mucosa is a critical step in the establishment of inhalational anthrax. Recent in vitro and in vivo studies indicated that this organism was able to penetrate the lung epithelium by directly entering into epithelial cells of the lung; however the molecular details of B. anthracis breaching the epithelium were lacking. Here, using a combination of pharmacological inhibitors, dominant negative mutants, and colocalization experiments, we demonstrated that internalization of spores by epithelial cells was actin-dependent and was mediated by the Rho-family GTPase Cdc42 but not RhoA or Rac1. Phosphatidylinositol 3-kinase (PI3K) activity was also required as indicated by the inhibitory effects of PI3K inhibitors, wortmannin and LY294002, and a PI3K dominant negative (DN) mutant Deltap85alpha. In addition, spore entry into epithelial cells (but not into macrophages) required the activity of Src as indicated by the inhibitory effect of Src family kinase (SFK) inhibitors, PP2 and SU6656, and specific siRNA knockdown of Src. Enrichment of PI3K and F-actin around spore attachment sites was observed and was significantly reduced by treatment with SFK and PI3K inhibitors, respectively. Moreover, B. anthracis translocation through cultured lung epithelial cells was significantly impaired by SFK inhibitors, suggesting that this signaling pathway is important for bacterial dissemination. The effect of the inhibitor on dissemination in vivo was then evaluated. SU6656 treatment of mice significantly reduced B. anthracis dissemination from the lung to distal organs and prolonged the median survival time of mice compared to the untreated control group. Together these results described a signaling pathway specifically required for spore entry into epithelial cells and provided evidence suggesting that this pathway is important for dissemination and virulence in vivo.
authors.affiliation: Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas, USA.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xue
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Jenkins
authors.forename: Chunfang
authors.initials: C
authors.lastname: Gu
authors.forename: Emanuel
authors.initials: E
authors.lastname: Smeds
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Ranga
authors.initials: R
authors.lastname: Vasan
authors.forename: Brooke H
authors.initials: BH
authors.lastname: Russell
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000378:metabolism*; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001408:Bacillus anthracis / Q000254:growth & development / Q000502:physiology*; D015153:Blotting, Western; D000081247:CSK Tyrosine-Protein Kinase; D002460:Cell Line; D002470:Cell Survival; D002867:Chromones / Q000494:pharmacology; D004847:Epithelial Cells / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D008856:Microscopy, Fluorescence; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011743:Pyrimidines / Q000494:pharmacology; D034741:RNA, Small Interfering; D013171:Spores, Bacterial / Q000254:growth & development / Q000502:physiology*; D013449:Sulfonamides / Q000494:pharmacology; D000077191:Wortmannin; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D000730:Androstadienes; D000081247:CSK Tyrosine-Protein Kinase; D002867:Chromones; D007211:Indoles; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases; D011743:Pyrimidines; D034741:RNA, Small Interfering; D013449:Sulfonamides; D000077191:Wortmannin; D019061:src-Family Kinases; C412373:AG 1879; C416927:SU 6656; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; C000631346:CSK protein, human