Targeting DNA double-strand breaks with TAL effector nucleases.
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
Christian
Michelle
M
Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA.
Cermak
Tomas
T
Doyle
Erin L
EL
Schmidt
Clarice
C
Zhang
Feng
F
Hummel
Aaron
A
Bogdanove
Adam J
AJ
Voytas
Daniel F
DF
D001665:Binding Sites / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D004247:DNA / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018390:Gene Targeting; D005818:Genetic Engineering; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014974:Xanthomonas; D016959:Xanthomonas campestris; D016335:Zinc Fingers / Q000235:genetics
D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004247:DNA; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific