title: Design and evaluation in mice of a broadly protective meningococcal group B native outer membrane vesicle vaccine.
abstract: A vaccine based on native outer membrane vesicles (NOMV) that has potential to provide safe, broad based protection against group B strains of Neisseria meningitidis has been developed. Three antigenically diverse group B strains of N. meningitidis were chosen and genetically modified to improve safety and expression of desirable antigens. Safety was enhanced by disabling three genes: synX, lpxL1, and lgtA. The vaccine strains were genetically configured to have three sets of antigens each with potential to induce protective antibodies against a wide range of group B strains. Preliminary immunogenicity studies with combined NOMV from the three strains confirmed the capacity of the vaccine to induce a broad based bactericidal antibody response. Analysis of the bactericidal activity indicated that antibodies to the LOS were responsible for a major portion of the bactericidal activity and that these antibodies may enhance the bactericidal activity of anti-protein antibodies.
authors.affiliation: Division of Bacterial and Rickettsial Diseases, 503 Robert Grant Ave., Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA. wendell.zollinger@gmail.com
authors.forename: Wendell D
authors.initials: WD
authors.lastname: Zollinger
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Donets
authors.forename: Deborah H
authors.initials: DH
authors.lastname: Schmiel
authors.forename: Valerian B
authors.initials: VB
authors.lastname: Pinto
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Labrie
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Moran
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Brandt
authors.forename: Boris
authors.initials: B
authors.lastname: Ionin
authors.forename: Ryan
authors.initials: R
authors.lastname: Marques
authors.forename: Max
authors.initials: M
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Mark B
authors.initials: MB
authors.lastname: Stoddard
authors.forename: Paul B
authors.initials: PB
authors.lastname: Keiser
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D055786:Gene Knockout Techniques; D022401:Meningococcal Vaccines / Q000276:immunology*; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000235:genetics* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022401:Meningococcal Vaccines