[Morphological characteristics in corneal smear of acanthamoeba keratitis].
OBJECTIVE
To study the morphological characteristics in corneal smear of acanthamoeba keratitis.


METHODS
Experimental study. From May 1991 to December 2007, in Department of Microbiology, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, 159 cases with acanthamoeba keratitis were analyzed for the laboratory results and clinical data. Giemsa's stained smear and wet-mount preparation of 159 cases were observed, all the results of cytology be photographed as the records.


RESULTS
Among the 159 cases with acanthamoeba keratitis, 131 patients (82.4%) were positive on smear, and 110 cases (69.2%) were positive in culture of amoeba. The detection positive rate of smear was apparently higher than culture. Typical trophozoites, pre-encystment trophozoites, mature cysts and empty cysts could be observed by smear. So it is an important method for the clinical diagnosis. The edema and dissolved corneal epithelial cells, exudate cells, arthrospores or chlamydoconidium of mycelial fungus, vacuoles, lipid droplet and medicine crystallization should be differentiated with the pathogen. The movement of trophozoites could mainly be observed through wet-mount preparation.


CONCLUSIONS
The detection of typical features of trophozoites, pre-encystment trophozoites, mature cysts and empty cysts by corneal smear is important for the etiological diagnosis of acanthamoeba keratitis.
Wang
Zhi-qun
ZQ
Beijing Institute of Ophthalmology, Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China.
Li
Ran
R
Zhang
Chen
C
Luo
Shi-yun
SY
Sun
Xu-guang
XG
Jin
Xiu-ying
XY
D000048:Acanthamoeba; D015823:Acanthamoeba Keratitis / Q000175:diagnosis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D003315:Cornea / Q000469:parasitology*; D005260:Female; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult