[Association between beta3-adrenergic receptor and oxidative stress in chronic heart failure rats].
OBJECTIVE
To investigate the association between beta(3)-adrenergic receptor (beta(3)-AR) and oxidative stress in isoproterenol (ISO)-induced chronic heart failure (HF) rats.


METHODS
Seven weight-matched normal adult rats (control), 18 ISO induced heart failure rats and 21 ISO induced heart failure rats treated with specific beta(3)-AR inhibitor, SR59230A for 6 weeks were included in this study. Echocardiography was performed at the end of the study and the myocardial levels of total superoxide dismutase (T-SOD) and lipid peroxidation (LPO) were measured by colorimetry, myocardial expression of beta(3)-AR was detected by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Compared with control group, the cardiac function was significantly reduced and myocardial beta(3)-AR mRNA expression was significantly increased, LPO level was also significantly enhanced while T-SOD level was significantly reduced in ISO group and these changes could be significantly attenuated by treatment with SR59230A.


CONCLUSIONS
Our results showed that myocardial upregulation of beta(3)-AR is associated with increased oxidative stress in this model and beta(3)-AR inhibitor may be a new therapeutic agent for heart failure treatment.
Kong
Yi-hui
YH
Department of Cardiology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Zhang
Ying
Y
Li
Na
N
Zhang
Li
L
Gao
Yan-hui
YH
Xue
Hong-jie
HJ
Li
Yue
Y
Li
Wei-min
WM
D000818:Animals; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000378:metabolism* / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D018384:Oxidative Stress; D011412:Propanolamines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D011412:Propanolamines; D022702:Receptors, Adrenergic, beta-3; D013482:Superoxide Dismutase