title: Emergence of dengue virus type 4 (genotype I) in India.
abstract: Dengue is an emerging arboviral disease and currently poses the greatest arboviral threat to human health. In recent decades, there has been a substantial increase in dengue outbreaks in many parts of the world including India. We performed an in-depth investigation of a major dengue outbreak in Andhra Pradesh, southern India in 2007 by serology, virus isolation, RT-PCR and genotyping. The results revealed an unusual emergence of dengue virus type 4 (DENV-4) along with the prevailing DENV-3. Phylogenetic analysis based on complete envelope gene of 182 globally diverse DENV-4 isolates demonstrated the involvement of a unique clade of genotype I of DENV-4 in the outbreak. This study also demonstrated a clear shift in the dominant serotype from DENV-3 to DENV-4 in India. This is the first report regarding the molecular characterization of Indian isolates of DENV-4, which has the potential to be involved in future outbreaks.
authors.affiliation: Division of Virology, Defence R & D Establishment, Jhansi Road, Gwalior, MP, India. pabandash@rediffmail.com
authors.forename: P K
authors.initials: PK
authors.lastname: Dash
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
authors.forename: S R
authors.initials: SR
authors.lastname: Santhosh
authors.forename: M M
authors.initials: MM
authors.lastname: Parida
authors.forename: M
authors.initials: M
authors.lastname: Neeraja
authors.forename: M V S
authors.initials: MV
authors.lastname: Subbalaxmi
authors.forename: V
authors.initials: V
authors.lastname: Lakshmi
authors.forename: P V L
authors.initials: PV
authors.lastname: Rao
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000821:virology*; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D004196:Disease Outbreaks; D005260:Female; D015752:Genes, env / Q000235:genetics; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D055815:Young Adult