A rapid and reliable bonding process for microchip electrophoresis fabricated in glass substrates.
In this report, we describe a rapid and reliable process to bond channels fabricated in glass substrates. Glass channels were fabricated by photolithography and wet chemical etching. The resulting channels were bonded against another glass plate containing a 50-microm thick PDMS layer. This same PDMS layer was also used to provide the electrical insulation of planar electrodes to carry out capacitively coupled contactless conductivity detection. The analytical performance of the proposed device was shown by using both LIF and capacitively coupled contactless conductivity detection systems. Efficiency around 47,000 plates/m was achieved with good chip-to-chip repeatability and satisfactory long-term stability of EOF. The RSD for the EOF measured in three different devices was ca. 7%. For a chip-to-chip comparison, the RSD values for migration time, electrophoretic current and peak area were below 10%. With the proposed approach, a single chip can be fabricated in less than 30 min including patterning, etching and sealing steps. This fabrication process is faster and easier than the thermal bonding process. Besides, the proposed method does not require high temperatures and provides excellent day-to-day and device-to-device repeatability.
Segato
Thiago Pinotti
TP
Instituto de Química de São Carlos, Universidade de São Paulo, São Paulo, Brazil.
Coltro
Wendell Karlos Tomazelli
WK
Almeida
André Luiz de Jesus
AL
Piazetta
Maria Helena de Oliveira
MH
Gobbi
Angelo Luiz
AL
Mazo
Luiz Henrique
LH
Carrilho
Emanuel
E
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D005898:Glass / Q000737:chemistry; D055616:Microtechnology / Q000379:methods*; D013997:Time Factors
D004129:Dimethylpolysiloxanes