title: Genotype variation of Trypanosoma cruzi isolates from different Brazilian biomes.
abstract: Chagas disease is an enzootic disease, in which the flagellate Trypanosoma cruzi infects a large variety of animals. Humans are accidentally infected due to the migration into wild environments. To identify T. cruzi discrete typing units (DTUs), 19 Brazilian isolates from different biomes and hosts were analyzed by PCR amplification of 24Sα rRNA, 18S rRNA and mini-exon gene sequences. The majority of the isolates was classified as TcIIb (TcII) but subtypes TcIIc (TcIII) and TcIId (TcV) were also identified. In addition, in monkeys TcI was detected.
authors.affiliation: Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21045-900, Brazil. cacaraujo@hotmail.com
authors.forename: C A C
authors.initials: CA
authors.lastname: Araújo
authors.forename: P J
authors.initials: PJ
authors.lastname: Waniek
authors.forename: S C C
authors.initials: SC
authors.lastname: Xavier
authors.forename: A M
authors.initials: AM
authors.lastname: Jansen
mesh_terms: D000818:Animals; D001938:Brazil; D014355:Chagas Disease / Q000469:parasitology*; D048293:Didelphis / Q000469:parasitology; D005091:Exons / Q000235:genetics; D005838:Genotype; D006801:Humans; D051039:Leontopithecus / Q000469:parasitology; D016133:Polymerase Chain Reaction; D018419:Primate Diseases / Q000469:parasitology; D011323:Primates; D012335:RNA, Ribosomal / Q000235:genetics; D012376:Rodent Diseases / Q000469:parasitology; D012377:Rodentia; D014225:Triatominae / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal