Genetic variation in natural populations of Stipa tenacissima from Algeria.
Intermicrosatellite PCR [inter-simple sequence repeat (ISSR)-PCR] markers and cytogenetics criteria were used to assess the level of genetic diversity and genetic structure in 17 populations of Stipa tenacissima (Gramineae) from Algeria. All populations sampled in the steppe area were diploids (2n = 2x = 24), and those sampled in the dry area were hexaploids (2n = 6x = 72). The dendrogram based on ISSR-PCR showed homogeneity within populations and large variability among populations. All individuals of the same population were gathered and formed groups clearly separated in all populations. These groups were separated into two clusters related to biotope, one from the steppe area and the other from the dry area. AMOVA indicated low genetic diversity among populations (30% of variation) and high within populations (70%). This variation pattern would constitute an adaptive strategy to grow in various ecological conditions.
Boussaid
Mohamed
M
Laboratoire d'agro biotechnologie et de nutrition en zone semi aride, Université Ibn Khaldoun, BP 78, Zaaroura, Tiaret, Algeria.
Benito
Cesar
C
Harche
Mériem Kaid
MK
Naranjo
Tomás
T
Zedek
Mohamed
M
D000462:Algeria; D032461:Chromosomes, Plant / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004777:Environment; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D018598:Minisatellite Repeats / Q000235:genetics; D010802:Phylogeny; D006109:Poaceae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D017931:DNA Primers; D005819:Genetic Markers