[The effect of temperature on the postmortem redistribution of bupivacaine in epidural anesthesia death dogs].
OBJECTIVE
To establish the models of postmortem redistribution(PMR) in dogs with epidural anesthesia and to investigate the effect of temperature on the PMR of Bupivacaine.


METHODS
Eighteen male dogs were executed by epidural anesthesia with a dose of 5 mg/kg bupivacaine hydrochloride and randomly divided into three groups, room temperature (20-23 degrees C) group, 4 degrees C group and -20 degrees C group. The cardiac blood, peripheral blood, liver and cerebrum were collected at 0, 2, 4, 8, 24, 48, 72, 96, 120h postmortem. The contents of bupivacaine in those samples were analyzed by GC-NPD and GC-MS, the difference among three groups were compared.


RESULTS
The bupivacaine PMR of room temperature group was evident and complex in cardiac blood, peripheral blood and cerebrum. The PMR of 4 degrees C group was weaker and slower than that of normal temperature group. The bupivacaine PMR of the -20 degrees C group was the weakest in three groups.


CONCLUSIONS
PMR of bupivacaine will happen in epidural anesthesia death dogs, but it could be delayed or prevent by low temperature storage.
Zhang
Gao-qin
GQ
School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
Wang
Da-li
DL
Yun
Ke-ming
KM
Zhang
Da-ming
DM
D015360:Analgesia, Epidural; D000779:Anesthetics, Local / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002045:Bupivacaine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D004285:Dogs; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008099:Liver / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D011180:Postmortem Changes; D013696:Temperature; D013997:Time Factors; D014018:Tissue Distribution
D000779:Anesthetics, Local; D002045:Bupivacaine