title: Picosecond primary structural transition of the heme is retarded after nitric oxide binding to heme proteins.
abstract: We investigated the ultrafast structural transitions of the heme induced by nitric oxide (NO) binding for several heme proteins by subpicosecond time-resolved resonance Raman and femtosecond transient absorption spectroscopy. We probed the heme iron motion by the evolution of the iron-histidine Raman band intensity after NO photolysis. Unexpectedly, we found that the heme response and iron motion do not follow the kinetics of NO rebinding. Whereas NO dissociation induces quasi-instantaneous iron motion and heme doming (<0.6 ps), the reverse process results in a much slower picosecond movement of the iron toward the planar heme configuration after NO binding. The time constant for this primary domed-to-planar heme transition varies among proteins (approximately 30 ps for myoglobin and its H64V mutant, approximately 15 ps for hemoglobin, approximately 7 ps for dehaloperoxidase, and approximately 6 ps for cytochrome c) and depends upon constraints exerted by the protein structure on the heme cofactor. This observed phenomenon constitutes the primary structural transition in heme proteins induced by NO binding.
authors.affiliation: Laboratoire d'Optique et Biosciences, Institut National de la Santé et de la Recherche Médicale, Ecole Polytechnique, 91128 Palaiseau, France. sergei.kruglik@upmc.fr
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Kruglik
authors.forename: Byung-Kuk
authors.initials: BK
authors.lastname: Yoo
authors.forename: Stefan
authors.initials: S
authors.lastname: Franzen
authors.forename: Marten H
authors.initials: MH
authors.lastname: Vos
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Martin
authors.forename: Michel
authors.initials: M
authors.lastname: Negrerie
mesh_terms: D000494:Allosteric Regulation; D006418:Heme / Q000737:chemistry* / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D007700:Kinetics; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013997:Time Factors
chemical_list: D006454:Hemoglobins; D009211:Myoglobin; D009569:Nitric Oxide; D006418:Heme