Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae.
The study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. The synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (HPLC). According to the reaction of s-6-a catalyzed by fructosyltransferase from Aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, pH, substrate and enzyme concentration in the reaction, were investigated. All results indicated that the fructosyltransferase could catalyze the s-6-a synthesis, and the optimal conditions of fructosyltransferase in reaction were 50°C, pH 6.2, 48h reaction time, 60% sucrose, 1:3 ratio of g-6-a to sucrose and 4.0mg/L concentration of enzyme. This study plays the important role in sucralose synthesis, because it is very cumbersome in the reported methods.
Han
Yawei
Y
School of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan Province, 450002, China.
Liu
Guoming
G
Huang
Dongye
D
Qiao
Baojian
B
Chen
Liping
L
Guan
Lihong
L
Mao
Duobin
D
D001236:Aspergillus oryzae / Q000201:enzymology*; D055162:Biocatalysis; D002851:Chromatography, High Pressure Liquid; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D013379:Substrate Specificity; D013395:Sucrose / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D001426:Bacterial Proteins
D006602:Hexosyltransferases; D013395:Sucrose; C556997:sucrose-6-acetate; C021451:inulosucrase; D001426:Bacterial Proteins