title: [Assessment of an immature platelet fraction (IFP%) in the diagnosis of thrombocytopenia].
abstract: Reticulated platelets are young platelets containing mRNA. They reflect the rate of thrombopoesis. The aim of this study was to evaluate the reliability of the percentage of reticulated platelets (IPF%) as a diagnostic test for thrombocytopenia pathogenesis. IPF% was measured using XE 2100 Sysmex. An IPF% reference range in 52 healthy individuals was established as 1-4.5% with a median 2.2%. In all the 13 patients with idiopathic thrombocytopenic purpura IPF% was increased (median 11.8, range 5.3-54.3%). Only 7 out of 18 patients with disseminated intravascular coagulation had high IPF% (median 5.4%, range 2.9-14.1%). Surprisingly, IPF% was increased in 17 out of 22 patients with acute leukaemia (median 9.7%, range 0.9-41.9%). In CIVD, IPF% values correlated with the severity of the illness. Increased values in acute leukaemia could not be explained by non specific staining but by delayed maturation of reticulated platelets. A high IPF% does not substantiate hyperdestructive thrombocytopenia but a diagnosis of idiopathic thrombocytopenic purpura should be questioned if IPF% is not raised.
authors.affiliation: Laboratoire d'hématologie, Hôpital de Cimiez, Nice.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cannavo
authors.forename: Corinne
authors.initials: C
authors.lastname: Ferrero-Vacher
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sudaka
authors.forename: Danielle
authors.initials: D
authors.lastname: Aquaronne
authors.forename: Frédéric
authors.initials: F
authors.lastname: Berthier
authors.forename: Sophie
authors.initials: S
authors.lastname: Raynaud
mesh_terms: D001331:Automation / Q000379:methods; D004396:Coloring Agents; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000175:diagnosis; D006801:Humans; D010976:Platelet Count / Q000379:methods*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000175:diagnosis; D012016:Reference Values; D015203:Reproducibility of Results; D017701:Reticulocyte Count / Q000379:methods; D013921:Thrombocytopenia / Q000097:blood / Q000175:diagnosis*
chemical_list: D004396:Coloring Agents