Therapeutic effects of hybrid liposomes on gastric carcinoma involve apoptosis.
Hybrid liposomes (HLs) composed of 90 mol% dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene(23)dodecyl ether (C(12)(EO)(23)), having a hydrodynamic diameter under 100 nm, were preserved for a period of one month. The inhibitory effects of HLs on the growth of gastric carcinoma (CoRa 622 G6) cells in vitro were investigated. Decrease in mitochondrial membrane potential and activation of caspase-8, caspase-9 and caspase-3 were observed, indicating that apoptotic signals induced by HLs should pass through mitochondria and these caspases. Remarkable inhibitory effects on the metastasis of gastric carcinoma along with apoptosis and prolonged survival were obtained for mouse models of gastric carcinoma with peritoneal dissemination after the treatment with HLs in vivo.
Ichihara
Hideaki
H
Division of Applied Life Science, Graduate School of Engineering, Sojo University 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Matsuoka
Yusuke
Y
Matsumoto
Yoko
Y
Ueoka
Ryuichi
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
D008081:Liposomes; D011092:Polyethylene Glycols; D020169:Caspases; C515901:Brij 35; D004134:Dimyristoylphosphatidylcholine