Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila.
Farnesylation involves covalent linkage of eukaryotic proteins to a lipid moiety to anchor them into membranes, which is essential for the biological function of Ras and other proteins. A large cadre of bacterial effectors is injected into host cells by intravacuolar pathogens through elaborate type III-VII translocation machineries, and many of these effectors are incorporated into the pathogen-containing vacuolar membrane by unknown mechanisms. The Dot/Icm type IV secretion system of Legionella pneumophila injects into host cells the F-box effector Ankyrin B (AnkB), which functions as platforms for the docking of polyubiquitinated proteins to the Legionella-containing vacuole (LCV) to enable intravacuolar proliferation in macrophages and amoeba. We show that farnesylation of AnkB is indispensable for its anchoring to the cytosolic face of the LCV membrane, for its biological function within macrophages and Dictyostelium discoideum, and for intrapulmonary proliferation in mice. Remarkably, the protein farnesyltransferase, RCE-1 (Ras-converting enzyme-1), and isoprenyl cysteine carboxyl methyltransferase host farnesylation enzymes are recruited to the LCV in a Dot/Icm-dependent manner and are essential for the biological function of AnkB. In conclusion, this study shows novel localized recruitment of the host farnesylation machinery and its anchoring of an F-box effector to the LCV membrane, and this is essential for biological function in vitro and in vivo.
Price
Christopher T D
CT
Department of Microbiology and Immunology, School of Medicine and 2 Department of Biology, University of Louisville, Louisville, KY 40292, USA.
Al-Quadan
Tasneem
T
Santic
Marina
M
Jones
Snake C
SC
Abu Kwaik
Yousef
Y
D000818:Animals; D017487:Ankyrins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D004023:Dictyostelium / Q000378:metabolism / Q000382:microbiology; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000382:microbiology*; D051231:Farnesyltranstransferase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005260:Female; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007425:Intracellular Membranes / Q000378:metabolism; D016952:Legionella pneumophila / Q000166:cytology / Q000502:physiology*; D007877:Legionnaires' Disease / Q000382:microbiology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D011496:Protein Methyltransferases / Q000235:genetics / Q000378:metabolism; D017368:Protein Prenylation / Q000187:drug effects / Q000502:physiology*; D021381:Protein Transport / Q000187:drug effects / Q000235:genetics; D034622:RNA Interference; D014162:Transfection; D020298:U937 Cells; D057149:Ubiquitinated Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism / Q000382:microbiology
D017487:Ankyrins; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D020662:Guanine Nucleotide Exchange Factors; D026901:Membrane Transport Proteins; C418483:SIL1 protein, human; D057149:Ubiquitinated Proteins; D011496:Protein Methyltransferases; C056501:protein-S-isoprenylcysteine O-methyltransferase; D051231:Farnesyltranstransferase; D010450:Endopeptidases; C491326:RCE1 protein, human