Molecular epidemiology of Japanese avian Pasteurella multocida strains by the single-enzyme amplified fragment length polymorphism and pulsed-field gel electrophoresis.
Molecular epidemiology analyses of the 36 clinical isolates of Pasteurella multocida from various avian hosts in Japan between 1976 to 2007 including 5 reference strains from the U.S.A., Taiwan and Indonesia were performed by employing the single-enzyme amplified fragment length polymorphism (SE-AFLP) comparison with the classical ApaI-based pulsed-field gel electrophoresis (PFGE). As the results, SE-AFLP gave 21 profiles while PFGE gave 20 profiles. The Simpson's index of diversity analysis indicated that SE-AFLP gave a high discrimination power than PFGE. This concluded that SE-AFLP is a higher discrimination power than PFGE to differentiate avian P. multocida isolates in Japan. In addition, the genetical profiles suggested that there is the evolution of somatic serotype 3 strain in the indigenous host of Japan.
Sthitmatee
Nattawooti
N
Laboratory of Veterinary Microbiology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. drneaw@gmail.com
Kataoka
Yasushi
Y
Sawada
Takuo
T
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000235:genetics*; D001717:Birds; D002645:Chickens; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D019143:Evolution, Molecular; D005260:Female; D005784:Gene Amplification; D014644:Genetic Variation; D007564:Japan / Q000453:epidemiology; D010326:Pasteurella Infections / Q000235:genetics* / Q000662:veterinary; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D012703:Serotyping; D014422:Turkeys; D014481:United States / Q000453:epidemiology