title: Assessment of physical process conditions for enhanced production of novel glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428.
abstract: Statistically based experimental design was applied to maximize the production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428. The effect of physical process parameters (initial pH of the medium, temperature, rpm of the shaking incubator, and inoculum size) on the production of L-asparaginase from P. carotovorum MTCC 1428 was studied using central composite design technique. The individual optimum levels of initial pH of the medium, temperature, rpm of shaking incubator, and inoculum size were found to be 6.90, 29.8 °C, 157 rpm, and 2.61% (v/v), respectively, for the production of L-asparaginase. After physical process parameters optimization, the production and productivity of L-asparaginase was enhanced by 26.39% (specific activity) and 10.19%, respectively. Maximization of L-asparaginase production was achieved at 12 h under optimal levels of physical process parameters in shake flask level.
authors.affiliation: Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Venkata Dasu
authors.initials: VD
authors.lastname: Veeranki
authors.forename: Kannan
authors.initials: K
authors.lastname: Pakshirajan
mesh_terms: D000704:Analysis of Variance; D001215:Asparaginase / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D005972:Glutaminase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D016973:Pectobacterium carotovorum / Q000201:enzymology*; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D001215:Asparaginase; D005972:Glutaminase