Evaluation of the SimPlate method for enumeration of Escherichia coli in swab samples from beef and lamb carcasses.
Abattoirs have to enumerate Escherichia coli on carcass surfaces as part of compulsory HACCP monitoring and they therefore need rapid and reliable methods. The objective of this study was to compare a conventional plating method with a faster, simpler method for detection and enumeration of E. coli in samples from naturally contaminated carcasses. The two methods were the conventional pour plate method of the Nordic Committee on Food Analysis; NMKL Method No. 125, and the enzymatic method of SimPlate Coliforms &E. coli. Materials were 588 cotton-cloth samples used for swabbing 100 cm(2) areas on four sites on cattle and lamb carcasses in three commercial abattoirs in Norway. E. coli was detected by at least one of the methods in 270 (46%) of the samples. Forty-five samples (8%) were positive only by SimPlate while 28 samples (5%) were positive only by NMKL125. Cohen's kappa value was 0.74 for detection/non-detection results, which showed a high level of agreement between the two methods. E. coli counts determined by the conventional NMKL125 method showed a high concordance correlation (ccc 0.80, slope 0.99) with most probable number (MPN) values obtained with SimPlate. SimPlate is a rapid and reliable method for detection and enumeration of E. coli and a suitable alternative method for use with swab samples from cattle and lamb carcasses.
Hauge
Sigrun J
SJ
Animalia Norwegian Meat and Poultry Research Centre, Oslo, Norway. sigrun.hauge@animalia.no
Nesbakken
Truls
T
Skjerve
Eystein
E
Dommarsnes
Kari
K
Østensvik
Øyvin
Ø
D000003:Abattoirs; D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development* / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000382:microbiology*; D009664:Norway; D012756:Sheep