title: Interphase fluorescence in situ hybridization on selected plasma cells is superior in the detection of cytogenetic aberrations in plasma cell dyscrasia.
abstract: Interphase fluorescence in situ hybridization (FISH) detects nonrandom cytogenetic abnormalities in plasma cell (PC) dyscrasia according to PC burden. However, when performed on cultured whole bone marrow (BM), it often fails to detect these aberrations. We have compared this interphase FISH technique with FISH after PC purification or identification to detect recurrent aberrations. In this study, 235 BM samples were collected from patients with multiple myeloma (MM) or related PC disorders regardless of disease status. All samples were analyzed in parallel. Clonal abnormalities were detected in 34.9% of cultured samples compared with 71.0% PC selected samples (P < 0.001). Moreover, FISH on PCs allowed to detect more abnormalities per case (P < 0.001) and identified higher percentages of abnormal nuclei (P < 0.001). This study indicates that FISH on PCs is the preferred technique for routine cytogenetic investigation of MM.
authors.affiliation: Centrum voor Menselijke Erfelijkheid, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Natalie
authors.initials: N
authors.lastname: Put
authors.forename: Heidi
authors.initials: H
authors.lastname: Lemmens
authors.forename: Iwona
authors.initials: I
authors.lastname: Wlodarska
authors.forename: Peter
authors.initials: P
authors.lastname: Konings
authors.forename: Yves
authors.initials: Y
authors.lastname: Moreau
authors.forename: Anne
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenberghe
authors.forename: Lucienne
authors.initials: L
authors.lastname: Michaux
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008875:Middle Aged; D010265:Paraproteinemias / Q000235:genetics*