title: Upregulation of human selenoprotein H in murine hippocampal neuronal cells promotes mitochondrial biogenesis and functional performance.
abstract: Overexpression of selenoprotein H (SelH) gene provides neuroprotection in neurons against UVB-induced cell death by blocking the mitochondrial-initiated apoptotic cell death pathway. This study examined the effects of SelH on mitochondrial biogenesis and mitochondrial function. The results demonstrated that overexpression of SelH gene in neuronal HT22 cells significantly increased the levels of mitochondrial biogenesis regulators, nuclear respiratory factor-1 (NRF-1), peroxisome proliferator-activated receptor-γ coactivator-1 alpha (PGC-1α) and mitochondrial transcription factor A (Tfam). Mitochondrial cytochrome c content was elevated, mass was increased and respiration was enhanced. SelH transfection ameliorated ultra violet B (UVB)-induced suppression of mitochondrial biogenesis markers and depolarization of mitochondrial membrane potential. Overexpression of SelH promotes mitochondrial biogenesis and improves mitochondrial functional performance.
authors.affiliation: Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technological Enterprise (BRITE) Building 2025, North Carolina Central University, 302 East Lawson Street, Durham, NC 27707, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mendelev
authors.forename: Suresh L
authors.initials: SL
authors.lastname: Mehta
authors.forename: Sam
authors.initials: S
authors.lastname: Witherspoon
authors.forename: Qingping
authors.initials: Q
authors.lastname: He
authors.forename: Jonathan Z
authors.initials: JZ
authors.lastname: Sexton
authors.forename: P Andy
authors.initials: PA
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D019069:Cell Respiration; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051266:Nuclear Respiratory Factor 1 / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D051266:Nuclear Respiratory Factor 1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D051140:Selenoproteins; C081930:Tfam protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C519118:selenoprotein H, human