[The effect of smoking and smoking cessation on the phosphorylation of IKK-ß in type 2 diabetic rats].
OBJECTIVE
To investigate the effect of smoking and smoking cessation on the phosphorylation of IKK-ß in type 2 diabetic rats.


METHODS
Forty-two six-week-old Wistar rats were randomly divided into 4 groups: normal control (NC, n = 7), diabetes control (DC, n = 7), diabetes with smoking (DS, n = 14) and diabetes with smoking cessation (SC, n = 14). Rats in DS and SC groups were further assigned randomly into 8w and 12w subgroups. DS group was given passive smoking twice a day for 8 or 12 weeks, while SC group ceased passive smoking for 4 weeks after 8 or 12 weeks of smoking. Western blot method was used to detect the level of IKK-ß phosphorylation in skeletal muscle.


RESULTS
Compared with the NC group, the phosphorylation of IKK-ß protein in DC group was increased (0.16 ± 0.05 vs 0.30 ± 0.08, P < 0.01). There was an increasing trend with the phosphorylation level of IKK-ß in the DS (8w) subgroup, but there was no statistical difference between the DC group and SC (8w) subgroup (0.40 ± 0.09 vs 0.30 ± 0.08, 0.36 ± 0.10, P > 0.05). The phosphorylation level of IKK-ß in DS (12w) group increased obviously, being significantly higher than that in the DC group and SC (12w) subgroup (0.74 ± 0.11 vs 0.30 ± 0.08, 0.35 ± 0.07, P < 0.01).


CONCLUSIONS
With the prolongation of smoking duration, the phosphorylation of IKK-ß in type 2 diabetic rats increased. After smoking cessation, the phosphorylation of IKK-ß decreased. The phosphorylation of IKK-ß may be involved in the mechanism by which smoking causes type 2 diabetes.
Liu
Hong
H
Department of Geriatric Endocrinology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. hongmm@163.net
Fang
Dan
D
Yue
Hui-fen
HF
Deng
Hong-ming
HM
Meng
Bi-hui
BH
Wen
Zhong-wei
ZW
Sun
Xiao-fei
XF
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D051550:I-kappa B Kinase / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking; D016540:Smoking Cessation
D051550:I-kappa B Kinase