[Microbiological contamination of environments and surfaces at commercial restaurants].
This study was carried out to provide subsidies for sanitary actions applied to manipulators, environments and surfaces, assessing levels of microbiological contamination in three commercial restaurants (A, B and C) in Viçosa, Minas Gerais State. Microbiological analysis were performed for presumptive counting of Bacillus cereus and mesophilic aerobic bacteria on surfaces of stainless steel benches, equipments, utensils and hands of the manipulators (swab technique), and air (simple sedimentation). It was observed expressive contamination by mesophilic aerobic bacteria in the samples examined, exceeding limits proposed by the APHA (American Public Health Association). Mesophilic aerobic microorganisms were detected in 100% of air samples, with counts ranging from 4.1 x 10(1) CFU/cm(2)/week to 1.1 x 10(3) CFU/cm(2)/week. Typical colonies of B. cereus were detected in 19% of all air samples, and the presence of such colonies was observed in all restaurants in levels that reached 2.1 x 10(1) CFU/cm(2)/week. In surfaces and hands examined it was also possible to isolate typical colonies of the pathogen in all restaurants. The sanitary situation of the environments studied requires interventions to reduce risks of great magnitude, concerning the occurrence of foodborne diseases.
Coelho
Ana Iris Mendes
AI
Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, MG, Brazil. aicoelho@ufv.br
Milagres
Regina Célia Rodrigues Miranda
RC
Martins
Joice de Fátima Laureano
Jde F
Azeredo
Raquel Monteiro Cordeiro de
RM
Santana
Angela Maria Campos
AM
D000391:Air Microbiology; D004866:Equipment Contamination; D006225:Hand / Q000382:microbiology; D006801:Humans; D012147:Restaurants / Q000592:standards*