[Sequencing of mitochondrial DNA CO II gene in carrion-breeding flies and larvae in Zhengzhou].
OBJECTIVE
Using CO II sequences to identify common species of carrion-breeding flies and larvae.


METHODS
flies and larvae were collected on the corpses of rats in Zhengzhou district, DNA was extracted, CO II sequences were amplified and sequenced. Clustalx and MEGA 4.0 software were used to analyze the gene sequences and to construct the phylogenetic trees.


RESULTS
There was no significant gene difference between adults and larvae. COII gene sequences could be used to identify Boettcherisca peregrina, Aldrichina grahami and Lucilia illustris but they could not distinguish Lucilia cuprina from the Lucilia sericata because of their close evolutionary distance and single nucleotide polymorphisms in aldrichina grahami and Lucilia illustris populations were found.


CONCLUSIONS
CO II sequence of mtDNA in Zhengzhou district can be used effectively to identify some common species of carrion-breeding fly. The method is simple and accurate.
Zhao
Zu-liang
ZL
Department of Forensic Medicine, Zhengzhou University, Zhengzhou 450001, China. zhaozuliang_5352@126.com
Yan
Hong-tao
HT
Li
Shi-cai
SC
Liu
Guang-xu
GX
Shen
Xin-tang
XT
D000818:Animals; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004772:Entomology; D005554:Forensic Medicine / Q000379:methods; D017344:Genes, Insect; D007814:Larva / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV