Enhanced expression of adenovirus encoding rhEPO assisted by BAPTA.
Infection efficiency is the key issue for gene delivery using adenovirus vector and usually unsatisfactory. In this study, recombinant adenoviruses encoding recombinant human EPO were prepared using the Adeasy system, and injected into the mammary gland of goats via the teat canal. BAPTA was used to treat the mammary gland to facilitate adenoviruses infection compared with EGTA. Milk serum was collected from the infected mammary gland and characterized by ELISAs and Western blotting. Expression level of rhEPO from the group treated by BAPTA was higher than that treated by EGTA.
Liu
Zhi-Bin
ZB
Northwest A&F University, Yangling, Shaanxi, China.
Han
Zeng-Sheng
ZS
Li
Qing-Wang
QW
Yang
Hai
H
Lu
Wen-Zong
WZ
Li
Wen-Ye
WY
D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002614:Chelating Agents / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D008892:Milk / Q000378:metabolism*; D011897:Random Allocation; D011994:Recombinant Proteins
D002614:Chelating Agents; D011994:Recombinant Proteins; D004921:Erythropoietin; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid