Influence of GSTs, CYP2E1 and mEH polymorphisms on 1, 3-butadiene-induced micronucleus frequency in Chinese workers.
1,3-butadiene (BD) has been classified as a human carcinogen, however, the relationship between chromosomal damage and its metabolic polymorphisms is not clear. The present study used the CBMN assay to detect chromosomal damage in the peripheral lymphocytes of 166 exposed workers and 41 non-exposed healthy individuals. PCR and PCR-RFLP were applied to detect GSTT1, GSTM1, CYP2E1 c1c2 and mEH Tyr113His, His139Arg polymorphisms. The results demonstrated that the micronucleus (MN) frequency of the exposed workers was significantly higher than controls (P<0.01). Among the exposed workers, the individuals with high BD exposures are more susceptible to chromosomal damage than those with low exposures (FR=1.30, 95% CI 1.14-1.53; P<0.05). Gender-difference was also found in our study: males got lower micronucleus frequency than females. Workers who carried the genotypes of GSTM1 (+), CYP2E1 (c1c2/c2c2) and mEH intermediate (I) group had significantly higher MN frequency than those carrying the genotypes of GSTM1 (-) (FR=1.29, 95% CI 1.05-1.59; P<0.05), CYP2E1 (c1c1) (FR=1.55, 95% CI 1.24-1.93; P<0.01) or mEH high (H) group (FR=1.57, 95% CI 1.08-2.34; P<0.05), respectively. Our data indicated that the current BD exposure level could cause significantly higher MN frequency in workers than controls. Polymorphisms of GSTM1, CYP2E1 and mEH are susceptible to altered chromosome damage.
Tan
Hongshan
H
Department of Occupational and Environmental medicine, School of Public Health, Fudan University, Shanghai 200032, China.
Wang
Qi
Q
Wang
Aihong
A
Ye
Yunjie
Y
Feng
Nannan
N
Feng
Xiaoqing
X
Lu
Lingeng
L
Au
William
W
Zheng
Yuxin
Y
Xia
Zhaolin
Z
D000328:Adult; D000395:Air Pollutants, Occupational / Q000493:pharmacokinetics / Q000633:toxicity*; D002070:Butadienes / Q000493:pharmacokinetics / Q000633:toxicity*; D016022:Case-Control Studies; D002681:China; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D000072467:Cytochrome P450 Family 2; D004247:DNA / Q000235:genetics; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced* / Q000706:statistics & numerical data; D012150:Polymorphism, Restriction Fragment Length; D012727:Sex Characteristics
D000395:Air Pollutants, Occupational; D002070:Butadienes; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; C110332:CYP2F1 protein, human; D000072467:Cytochrome P450 Family 2; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases; C031763:1,3-butadiene