Selective culture of different types of human parotid gland cells.
BACKGROUND
Advances in salivary gland tissue engineering can benefit patients diagnosed with xerostomia. Complexity of the gland explains the urgent demand for a reliable protocol to isolate and expand various gland cells that can be used for further study.


METHODS
Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level.


RESULTS
Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique.


CONCLUSIONS
The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies.
Chan
Yen-Hui
YH
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
Huang
Tsung-Wei
TW
Young
Tai-Horng
TH
Lou
Pei-Jen
PJ
D000704:Analysis of Variance; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010306:Parotid Gland / Q000166:cytology* / Q000648:ultrastructure*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D023822:Tissue Engineering; D014987:Xerostomia / Q000209:etiology / Q000473:pathology / Q000628:therapy