title: Facile synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and application for lipase immobilization.
abstract: In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process.
authors.affiliation: College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Cui
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Lei
authors.forename: Lincheng
authors.initials: L
authors.lastname: Zhou
authors.forename: Fei
authors.initials: F
authors.lastname: Pan
mesh_terms: D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D017743:Equipment Reuse; D005976:Glutaral / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D012821:Silanes / Q000737:chemistry; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D000069463:Olive Oil; D010938:Plant Oils; D012821:Silanes; C040733:tetraethoxysilane; D008049:Lipase; D005976:Glutaral