title: Overexpression of rusticyanin in Acidithiobacillus ferrooxidans ATCC19859 increased Fe(II) oxidation activity.
abstract: A wide-host-range plasmid pTRUS containing a homologous rus gene under the control of Ptac promoter was constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer method to generate the engineered strain of A. ferrooxidans(pTRUS). The plasmid-based recombinant rus gene was successfully expressed. According to the results of real-time quantitative PCR assay, not only the transcription level of recombinant rus gene but also the transcription levels of the other genes of rus operon (cyc1, orf, coxB, coxA) were increased in A. ferrooxidans(pTRUS). The ferrous ion (Fe(2+)) oxidation activity was increased in A. ferrooxidans(pTRUS).
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jianqun
authors.initials: J
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Pang
authors.forename: Shuang
authors.initials: S
authors.lastname: Cui
authors.forename: Shuang
authors.initials: S
authors.lastname: Mi
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lin
mesh_terms: D042763:Acidithiobacillus / Q000235:genetics* / Q000378:metabolism*; D001400:Azurin / Q000096:biosynthesis*; D003227:Conjugation, Genetic; D005296:Ferrous Compounds / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D010084:Oxidation-Reduction; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
chemical_list: D005296:Ferrous Compounds; D012333:RNA, Messenger; C012543:rusticyanin; D001400:Azurin