Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay.
Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.
Saito
Takashi
T
Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.
Chibazakura
Taku
T
Takahashi
Kiwamu
K
Yoshikawa
Hirofumi
H
Sekine
Yasuhiko
Y
D002469:Cell Separation; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics*
D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins