Protective effects of kappa-ca3000+CP against ultraviolet-induced damage in HaCaT and MEF cells.
In this study, the complex kappa-ca3000+CP combined collagen peptide with kappa-carrageenan oligosaccharide was tested for its ability to moderate UV-induced damage and investigated for its protective mechanism against UV radiation. Human keratinocytes (HaCaT) and mouse embryonic fibroblasts (MEF) were used to monitor the effects of kappa-ca3000+CP on cell viability, apoptosis, level of collagen I and MMP-1, MAPKs activation and intracellular ROS production after UV-irradiation. The results indicated that application of the kappa-ca3000+CP (100 microg/ml) could significantly attenuate UV-induced HaCaT and MEF death, as well as inhibit the UV-induced apoptosis of HaCaT cells. The decreased collagen I synthesis and the increased MMP-1 expression of MEF by UV radiation were almost restored back to normal level after treatment with kappa-ca3000+CP. Moreover, kappa-ca3000+CP could significantly suppress UV-induced MAPKs activation and intracellular ROS production. Taken together, these results showed that antioxidant property of kappa-ca3000+CP can effectively attenuate UV-caused cell damage and skin photoaging by suppressing cell apoptosis and expression of MMP-1 through the MAPKs signaling pathways. Thus, kappa-ca3000+CP has potential antiaging effects and prominent protective effects on UV-induced skin cell damages, which might be used in pharmaceutical and cosmetic industries.
Ren
Shu-Wen
SW
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, People's Republic of China.
Li
Jing
J
Wang
Wei
W
Guan
Hua-Shi
HS
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence; D003094:Collagen / Q000494:pharmacology*; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays
D024042:Collagen Type I; D017382:Reactive Oxygen Species; D002351:Carrageenan; D003094:Collagen; D020929:Mitogen-Activated Protein Kinase Kinases; D020781:Matrix Metalloproteinase 1