title: Increased programmed death-ligand-1 expression in human gastric epithelial cells in Helicobacter pylori infection.
abstract: B7-H1 [programmed death-ligand-1 (PD-L1)] is a B7-family member that binds to programmed death-1 (PD-1). Recently, deficiency of PD-L1 has been demonstrated to result in accelerated gastric epithelial cell damage in gastritis, and PD-L1 is suggested to play a critical role in regulating T cell homeostasis. Here, we aimed to gain more insight into gastric PD-L1 expression, regulation and function during Helicobacter pylori infection. PD-L1 expression in human gastric epithelial cells was analysed using Western blotting, quantitative polymerase chain reaction and fluorescence activated cell sorter analysis. Furthermore, co-culture experiments of human gastric epithelial cells with primary human T cells or Jurkat T cells were conducted. PD-L1 expression in primary human gastric epithelial cells was strongly enhanced by H. pylori infection and activated T cells, and augmented markedly by further stimulation with interferon-γ or tumour necrosis factor-α. Moreover, PD-L1 expression in gastric epithelial cells significantly induced apoptosis of T cells. Our results indicate that a novel bidirectional interaction between human gastric epithelial cells and lymphocytes modulates PD-L1 expression in human gastric epithelial cells, contributing to the unique immunological properties of the stomach.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University and Hospital, Taichung, Taiwan.
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Wu
authors.forename: C-W
authors.initials: CW
authors.lastname: Lin
authors.forename: K-S
authors.initials: KS
authors.lastname: Cheng
authors.forename: C
authors.initials: C
authors.lastname: Lin
authors.forename: Y-M
authors.initials: YM
authors.lastname: Wang
authors.forename: I-T
authors.initials: IT
authors.lastname: Lin
authors.forename: Y-H
authors.initials: YH
authors.lastname: Chou
authors.forename: P-N
authors.initials: PN
authors.lastname: Hsu
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D060890:B7-H1 Antigen; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D016480:Helicobacter pylori / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D011706:Pyloric Antrum / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma