title: Structured triacylglycerol containing behenic and oleic acids suppresses triacylglycerol absorption and prevents obesity in rats.
abstract: BACKGROUND
  Dietary 1(3)-behenoyl-2,3(1)-dioleoyl-rac-glycerol (BOO) has been reported to inhibit pancreatic lipase activity in vitro and suppress postprandial hypertriacylglycerolemia in humans. In the present study, the anti-obesity activities of BOO and its inhibitory effects on lymphatic triacylglycerol (TAG) absorption were investigated in rats.
  
  
  METHODS
  In Experiment 1, rats were fed either BOO or soybean oil (SO) diet for 6 weeks. In the BOO diet, 20% of SO was replaced with an experimental oil rich in BOO. In Experiments 2 and 3, rats cannulated in the thoracic duct were administered an emulsions containing trioleoylglycerol (OOO) or an oil mixture (OOO:BOO, 9:1). Tri[1-14C]oleoylglycerol (14C-OOO) was added to the emulsions administered in Experiment 3.
  
  
  RESULTS
  No observable differences were detected in food intake or body weight gain between the BOO and SO groups in Experiment 1. Plasma and liver TAG concentrations and visceral fat weights were significantly lower in the BOO group than in the SO group. The apparent absorption rate of fat was significantly lower in the BOO group than in the SO group. In Experiment 2, the lymphatic recovery of oleic and behenic acids was significantly lower at 5 and 6 h after BOO administration than after OOO administration. In Experiment 3, the lymphatic recovery of 14C-OOO was significantly lower at 5 and 6 h after BOO administration than after OOO administration.
  
  
  CONCLUSIONS
  These results suggest that BOO prevents deposition of visceral fat and hepatic TAG by lowering and delaying intestinal absorption of TAG.
authors.affiliation: Food Science Research Institute, Fuji Oil Co., Ltd., 4-3 Kinunodai, Tsukubamirai-shi, Ibaraki 300-2497, Japan. kojima.makiko@so.fujioil.co.jp
authors.forename: Makiko
authors.initials: M
authors.lastname: Kojima
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tachibana
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamahira
authors.forename: Satoshi
authors.initials: S
authors.lastname: Seino
authors.forename: Ayako
authors.initials: A
authors.lastname: Izumisawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sagi
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Arishima
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Kohno
authors.forename: Kiyoharu
authors.initials: K
authors.lastname: Takamatsu
authors.forename: Motohiko
authors.initials: M
authors.lastname: Hirotsuka
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000138:chemical synthesis / Q000627:therapeutic use*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D004435:Eating; D019358:Fat Substitutes / Q000138:chemical synthesis / Q000627:therapeutic use; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D015228:Hypertriglyceridemia / Q000097:blood / Q000517:prevention & control; D007408:Intestinal Absorption; D050152:Intra-Abdominal Fat / Q000473:pathology; D007700:Kinetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008196:Lymph / Q000378:metabolism; D008208:Lymphatic System / Q000502:physiology; D008297:Male; D009765:Obesity / Q000097:blood / Q000178:diet therapy / Q000473:pathology / Q000517:prevention & control*; D019301:Oleic Acid / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood / Q000138:chemical synthesis / Q000378:metabolism* / Q000627:therapeutic use*; D015430:Weight Gain
chemical_list: D019440:Anti-Obesity Agents; D019358:Fat Substitutes; D005227:Fatty Acids; D014280:Triglycerides; D019301:Oleic Acid; D002784:Cholesterol; C007547:behenic acid