Bisphenol A modulates expression of gonadotropin subunit genes in the hermaphroditic fish, Kryptolebias marmoratus.
Pituitary gonadotropins (GTHs), follicle stimulating hormone beta (FSH-beta), and luteinizing hormone beta (LH-beta) are the key hormones in the hypothalamus-pituitary-gonad (HPG) axis, and form the heterodimers between a common alpha subunit (gonadotropin-alpha) and FSH-beta and/or LH-beta. To obtain a better understanding on the modulation of gonadotropin subunit genes expression upon bisphenol A (BPA) exposure in hermaphroditic fish, we studied differential regulation of gonadotropin subunit genes from Kryptolebias marmoratus after the exposure of several EDCs. Expression profiles of these three genes when using quantitative real-time RT-PCR revealed that brain/pituitary tissues were highly expressed in these genes compared to other tissues. At different developmental stages, expression of those genes dramatically increased over the course of development but showed a decrease in expression at the secondary male (showing atresia) stage. When adult fish were exposed to BPA (600 microg/L for 96 h), a significant upregulation of these three genes was observed in the brain/pituitary. A time course study also revealed the increased expression of gonadotropin subunit genes over 12 h with a more pronounced effect on the expression of FSH-beta and LH-beta genes, indicating that both genes were associated with the BPA exposure on the transcriptional regulation. This is the first report of gonadotropin subunit genes from K. marmoratus, with particular emphasis on the modulation of their expressions by EDCs. In addition, these findings suggest that EDCs modulate the expression of gonadotropin subunit genes and would act as potential biomarkers upon EDCs exposure.
Rhee
Jae-Sung
JS
Department of Molecular and Environmental Bioscience, Graduate School, Hanyang University, Seoul 133-791, South Korea.
Kim
Ryeo-Ok
RO
Seo
Jung Soo
JS
Kang
Han Seung
HS
Park
Chang-Beom
CB
Soyano
Kiyoshi
K
Lee
Jehee
J
Lee
Young-Mi
YM
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001559:Benzhydryl Compounds; D003532:Cyprinodontiformes / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D037201:Follicle Stimulating Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006065:Gonadotropins, Pituitary / Q000235:genetics* / Q000378:metabolism; D057850:Hermaphroditic Organisms / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010636:Phenols / Q000633:toxicity*; D010802:Phylogeny; D013629:Tamoxifen / Q000633:toxicity; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004958:Estradiol; D004968:Estrogens, Non-Steroidal; D037201:Follicle Stimulating Hormone, beta Subunit; D006065:Gonadotropins, Pituitary; D037101:Luteinizing Hormone, beta Subunit; D010636:Phenols; D013629:Tamoxifen; C006780:bisphenol A; D000098826:Bisphenol A Compounds