Study on poly(L-lactide-co-trimethylene carbonate): synthesis and cell compatibility of electrospun film.
The biomedical applications of poly(l-lactide) (PLLA) were limited by its high crystallinity. In this paper, the copolymerization of trimethylene carbonate (TMC) and l-lactide (LLA) was carried out to improve the flexibility of PLLA. The effects of feeding dose, reaction temperature and polymerization time were investigated, and the copolymers were characterized with (1)H nuclear magnetic resonance, Fourier transform infrared reflection, gel permeation chromatography differential scanning calorimetry, thermogravimetric analysis and x-ray diffraction. The copolymers were electrospun to form porous films to study their cell compatibility. The results showed that the composition of the copolymer was nearly the same as that in the feeding dose, and the molecular weight of the copolymer decreased with increasing TMC content. The decrease in the reaction temperature and polymerization time would increase the molecular weight, but the composition deviates from the feeding dose. NIH/3T3 mouse fibroblast cells were cultured on the electrospun films. The morphology and proliferation of the cells were studied. The results implied that the cell compatibility of poly(l-lactide-co-trimethylene carbonate) copolymer was much better than that of the PLLA homopolymer.
Ji
Li-Jie
LJ
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People's Republic of China.
Lai
Kui-Lin
KL
He
Bin
B
Wang
Gang
G
Song
Li-Qing
LQ
Wu
Yao
Y
Gu
Zhong-Wei
ZW
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D004146:Dioxanes / Q000737:chemistry* / Q000494:pharmacology*; D004563:Electrochemistry / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D012399:Rotation
D001672:Biocompatible Materials; D004146:Dioxanes; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C443643:trimethylene carbonate; C033616:poly(lactide)