title: Modeling the electrophoresis and transport of peptides: the effective sphere model and complex formation.
abstract: Modeling the electrophoretic mobility of peptides is examined in this study using a "coarse grained" bead model, or B model for short 8 and also a simpler "effective sphere" (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013 M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA 30302-4098, USA sallison@gsu.edu
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Allison
authors.forename: Hongxia
authors.initials: H
authors.lastname: Pei
authors.forename: Michelle
authors.initials: M
authors.lastname: Allen
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Brown
authors.forename: Chang-Il
authors.initials: CI
authors.lastname: Kim
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhen
mesh_terms: D001692:Biological Transport; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010455:Peptides / Q000302:isolation & purification*
chemical_list: D010455:Peptides