Fast multidimensional localized parallel NMR spectroscopy for the analysis of samples.
A parallel localized spectroscopy (PALSY) method is presented to speed up the acquisition of multidimensional NMR (nD) spectra. The sample is virtually divided into a discrete number of nonoverlapping slices that relax independently during consecutive scans of the experiment, affording a substantial reduction in the interscan relaxation delay and the total experiment time. PALSY was tested for the acquisition of three experiments 2D COSY, 2D DQF-COSY and 2D TQF-COSY in parallel, affording a time-saving factor of 3-4. Some unique advantages are that the achievable resolution in any dimension is not compromised in any way: it uses conventional NMR data processing, it is not prone to generate spectral artifacts, and once calibrated, it can be used routinely with these and other combinations of NMR spectra.
Vega-Vazquez
Marino
M
Unidad de Resonancia Magnética, RIAIDT, Edif. CACTUS, Campus Sur, Universidad de Santiago de Compostela, Santiago de Compostela, A Coruña 15706, Spain.
Cobas
Juan Carlos
JC
Martin-Pastor
Manuel
M
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000737:chemistry; D013997:Time Factors
D009711:Nucleotides; D013395:Sucrose