title: An MRI-based atlas and database of the developing mouse brain.
abstract: The advent of mammalian gene engineering and genetically modified mouse models has led to renewed interest in developing resources for referencing and quantitative analysis of mouse brain anatomy. In this study, we used diffusion tensor imaging (DTI) for quantitative characterization of anatomical phenotypes in the developing mouse brain. As an anatomical reference for neuroscience research using mouse models, this paper presents DTI based atlases of ex vivo C57BL/6 mouse brains at several developmental stages. The atlas complements existing histology and MRI-based atlases by providing users access to three-dimensional, high-resolution images of the developing mouse brain, with distinct tissue contrasts and segmentations of major gray matter and white matter structures. The usefulness of the atlas and database was demonstrated by quantitative measurements of the development of major gray matter and white matter structures. Population average images of the mouse brain at several postnatal stages were created using large deformation diffeomorphic metric mapping and their anatomical variations were quantitatively characterized. The atlas and database enhance our ability to examine the neuroanatomy in normal or genetically engineered mouse strains and mouse models of neurological diseases.
authors.affiliation: Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Nelson
authors.initials: N
authors.lastname: Chuang
authors.forename: Susumu
authors.initials: S
authors.lastname: Mori
authors.forename: Akira
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hangyi
authors.initials: H
authors.lastname: Jiang
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Richards
authors.forename: Jeremy
authors.initials: J
authors.lastname: Nathans
authors.forename: Michael I
authors.initials: MI
authors.lastname: Miller
authors.forename: Arthur W
authors.initials: AW
authors.lastname: Toga
authors.forename: Richard L
authors.initials: RL
authors.lastname: Sidman
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000254:growth & development*; D001931:Brain Mapping / Q000379:methods*; D003391:Cranial Nerves / Q000033:anatomy & histology / Q000254:growth & development; D056324:Diffusion Tensor Imaging / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure