The enrichment of trophoblast giant cells from mid-gestation bovine placentae using fluorescence-activated cell sorting.
The objective of this work was to determine if fluorescence-activated cell sorting (FACS) can be used to isolate trophoblast giant cells (TGCs) from bovine placentae. Cotyledons were harvested, minced and digested with dispase/pancreatin. Tissue homogenates were incubated with Vybrant Dye Cycle-Green. FACS was completed, and cells with DNA content 2- to 4-times greater than cells within the diploid peak contained between 65 and 84% TGCs. The relative abundance of CSH1 and PAG1 transcripts were greater (P < 0.05) in TGC-enriched fractions than cells within the diploid peak. These observations indicate that FACS can effectively enrich TGCs from bovine placentae.
Pennington
K A
KA
Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611, USA.
Oliveira
L J
LJ
Yang
Q E
QE
Ealy
A D
AD
D000818:Animals; D002417:Cattle; D005260:Female; D005434:Flow Cytometry; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology*