title: Multiplexed immunoassays for the analysis of breast cancer biopsies.
abstract: Within the last decade, protein microarray technology has been successfully used for the simultaneous quantification of target proteins from minimal amounts of samples in basic and applied proteome research. The robustness and appropriate sensitivity of these miniaturized assays have been demonstrated and thus the transfer to routine and high-throughput applications is now possible. In this study, multiplexed bead-based sandwich immunoassays were used to determine the concentrations of 54 protein analytes, including HER 2 and the estrogen receptor, from ultrasound-guided large-core needle biopsies (LCNBs) from breast cancer patients. Expression levels for HER 2, estrogen receptors and progesterone receptors were also assessed by immunohistochemical routine staining, performed in the clinic on corresponding biopsy samples. The high concordance of the data sets generated with the bead-based protein arrays and by conventional immunohistochemical assessment of HER 2 and the estrogen receptor expressed by breast cancer cells present in the biopsies was demonstrated.
authors.affiliation: NMI, Natural and Medical Sciences Institute, University of Tuebingen, Markwiesenstrasse 55, 72770 Reutlingen, Germany. schneiderhan@nmi.de
authors.forename: Nicole
authors.initials: N
authors.lastname: Schneiderhan-Marra
authors.forename: Georg
authors.initials: G
authors.lastname: Sauer
authors.forename: Cornelia
authors.initials: C
authors.lastname: Kazmaier
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
authors.forename: Karin
authors.initials: K
authors.lastname: Koretz
authors.forename: Helmut
authors.initials: H
authors.lastname: Deissler
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Joos
mesh_terms: D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D011506:Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human