title: Classical swine fever virus NS3 is an IRES-binding protein and increases IRES-dependent translation.
abstract: To get more evidences for understanding the role of NS3 in viral translation, we observed the promotive effect of CSFV NS3 on IRES-mediated translation by using dicistronic and monocistronic systems containing the precise segment comprising CSFV IRES. The results for affinity chromatography and UV-crosslinking assays indicated that NS3 bound CSFV IRES and that CSFV NS5A and NS5B could reduce the IRES-NS3 interaction. Further experiments showed that the NS5A also bound the IRES and that NS3 and NS5A bound the same binding sites of the IRES, suggesting that NS3 and NS5A competitively bind the same sites in IRES RNA sequence, thus hampering the interaction CSFV NS3 and IRES. But, CSFV NS5B was not found to interact with the IRES. The inhibitive effect of NS5B on binding of CSFV NS3 to IRES was supposed to result from the NS3-NS5B interaction which has been documented.
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Zailing
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yujing
authors.initials: Y
authors.lastname: Wang
authors.forename: Jialin
authors.initials: J
authors.lastname: Yu
authors.forename: Lingzhu
authors.initials: L
authors.lastname: Wan
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001665:Binding Sites; D006692:Classical Swine Fever Virus / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D011485:Protein Binding; D014176:Protein Biosynthesis; D020365:RNA Helicases / Q000037:antagonists & inhibitors / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D020365:RNA Helicases; D012367:RNA, Viral; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C096041:NS5 protein, flavivirus