title: Hedgehog signaling regulates segment formation in the annelid Platynereis.
abstract: Annelids and arthropods share a similar segmented organization of the body whose evolutionary origin remains unclear. The Hedgehog signaling pathway, prominent in arthropod embryonic segment patterning, has not been shown to have a similar function outside arthropods. We show that the ligand Hedgehog, the receptor Patched, and the transcription factor Gli are all expressed in striped patterns before the morphological appearance of segments in the annelid Platynereis dumerilii. Treatments with small molecules antagonistic to Hedgehog signaling disrupt segment formation. Platynereis Hedgehog is not necessary to establish early segment patterns but is required to maintain them. The molecular similarity of segment patterning functions of the Hedgehog pathway in an annelid and in arthropods supports a common origin of segmentation in protostomes.
authors.affiliation: Centre de Génétique Moléculaire du CNRS, FRE 3144, Avenue de la Terrasse, 91189 Gif-sur-Yvette, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Dray
authors.forename: Kristin
authors.initials: K
authors.lastname: Tessmar-Raible
authors.forename: Martine
authors.initials: M
authors.lastname: Le Gouar
authors.forename: Laura
authors.initials: L
authors.lastname: Vibert
authors.forename: Foteini
authors.initials: F
authors.lastname: Christodoulou
authors.forename: Katharina
authors.initials: K
authors.lastname: Schipany
authors.forename: Aurélien
authors.initials: A
authors.lastname: Guillou
authors.forename: Juliane
authors.initials: J
authors.lastname: Zantke
authors.forename: Heidi
authors.initials: H
authors.lastname: Snyman
authors.forename: Julien
authors.initials: J
authors.lastname: Béhague
authors.forename: Michel
authors.initials: M
authors.lastname: Vervoort
authors.forename: Detlev
authors.initials: D
authors.lastname: Arendt
authors.forename: Guillaume
authors.initials: G
authors.lastname: Balavoine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005075:Biological Evolution; D019521:Body Patterning / Q000187:drug effects; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D000072079:Patched Receptors; D010802:Phylogeny; D010879:Piperazines / Q000494:pharmacology; D011077:Polychaeta / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014704:Veratrum Alkaloids / Q000494:pharmacology
chemical_list: D053823:Hedgehog Proteins; D000072079:Patched Receptors; D010879:Piperazines; D011720:Pyrazoles; D011956:Receptors, Cell Surface; C468919:SANT-1 compound; D014157:Transcription Factors; D014704:Veratrum Alkaloids; C000541:cyclopamine