title: Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species.
abstract: We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.
authors.affiliation: Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.
authors.forename: Lindsay G
authors.initials: LG
authors.lastname: Stevenson
authors.forename: Steven K
authors.initials: SK
authors.lastname: Drake
authors.forename: Yvonne R
authors.initials: YR
authors.lastname: Shea
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Zelazny
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Murray
mesh_terms: D006801:Humans; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry* / Q000145:classification*