title: Direct typing and molecular evolutionary analysis of field samples of foot-and-mouth disease virus collected in Viet Nam between 2006 and 2007.
abstract: In this study, we used universal or duplex serotype-specific (O and Asia 1) RT-PCR to analyze clinical field samples of foot-and-mouth disease virus (FMDV) or virus isolates collected in Viet Nam between 2006 and 2007. We found viral serotypes O and Asia 1 circulating concurrently during this period. Direct sequencing of type-specific RT-PCR products revealed the existence of three different topotypes of serotype O: Southeast Asia (SEA), Middle East-South Asia (ME-SA), and Cathay. Of these, SEA was most prevalent during the period. All samples of serotype Asia 1 belonged to genetic group V. Based on the rooted maximum likelihood phylogenetic trees inferred from the VP1 region, new lineages in topotype SEA were originating from Viet Nam, and group V strains of Asia 1 have undergone fewer passages from the common ancestor, compared with other genetic groups. The co-circulation of different types of FMDV may complicate the individual or population genomic structures of FMDV and make conventional multiplex diagnostic methods and phylogenetic analyses with relevant evolutionary models essential in Viet Nam.
authors.affiliation: National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 430-824, Republic of Korea.
authors.forename: Kwang-Nyeong
authors.initials: KN
authors.lastname: Lee
authors.forename: Tung
authors.initials: T
authors.lastname: Nguyen
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Hoa T
authors.initials: HT
authors.lastname: Do
authors.forename: Huong T
authors.initials: HT
authors.lastname: Ngo
authors.forename: Duong T
authors.initials: DT
authors.lastname: Mai
authors.forename: Seo-Yong
authors.initials: SY
authors.lastname: Lee
authors.forename: Cam V
authors.initials: CV
authors.lastname: Nguyen
authors.forename: Sook Hee
authors.initials: SH
authors.lastname: Yoon
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity; D014744:Vietnam / Q000453:epidemiology