title: Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay.
abstract: Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.
authors.affiliation: Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Saito
authors.forename: Taku
authors.initials: T
authors.lastname: Chibazakura
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sekine
mesh_terms: D002469:Cell Separation; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins