title: [Expression of human beta-defensin after endoscopic sinus surgery for chronic sinusitis].
abstract: OBJECTIVE
  To observe the expressions of human beta-defensin 1 and 2 (hBD-1 and hBD-2) in nasal mucosa before and after the endoscopic sinus surgery and investigate the effects of hBD-1 and hBD-2 on the healing process after the surgery.
  
  
  METHODS
  The patients undergoing endoscopic sinus surgery for chronic sinusitis were divided into 3 groups according to the response to the surgery, namely cured group, response group and non-response group. With those from healthy control subjects as the control, nasal mucosa samples were collected from the patients at 1 week, 2 weeks, 1 month, 3 months and 6 months after the surgery for detection of hBD-1 and hBD-2 mRNA and protein expressions by RT-PCR and Western blotting.
  
  
  RESULTS
  hBD-1 and hBD-2 were expressed in both normal and chronic sinusitis mucosa, but the expression levels varied significantly between the individuals. The expression levels of hBD-2 was significantly correlated to the patients' response to the surgical treatment (P<0.05). hBD-1 showed slight differences between the individuals, but was not associated with the patients' prognosis.
  
  
  CONCLUSIONS
  The expressions of hBD-2 mRNA and protein are significantly increased in patients with good response to endoscopic sinus surgery for chronic sinusitis, suggesting the value of hBD-2 as an indicator of the patients' prognosis.
authors.affiliation: Department of Otolaryngology and Head-Neck Surgery, Yangang Hospital, Shenzhen 518083, China. xcz1962@126.com
authors.forename: Chu-zhi
authors.initials: CZ
authors.lastname: Xiao
authors.forename: Guang-xiang
authors.initials: GX
authors.lastname: He
authors.forename: Wei-guang
authors.initials: WG
authors.lastname: Deng
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
mesh_terms: D016022:Case-Control Studies; D002908:Chronic Disease; D004724:Endoscopy; D006801:Humans; D009297:Nasal Mucosa / Q000378:metabolism*; D011184:Postoperative Period; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012852:Sinusitis / Q000378:metabolism* / Q000601:surgery; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins