Erythroid induction of chronic myelogenous leukemia K562 cells following treatment with a photoproduct derived from the UV-A irradiation of 5-methoxypsoralen.
Induction of terminal erythroid differentiation can be an efficient strategy to inhibit proliferation of chronic myelogenous leukemia cells. Psoralens, well-known photo-chemotherapeutic agents, were found to be efficient at inducing erythroid differentiation of K562 cells, an in vitro cell line isolated from the pleural effusion of a patient with chronic myelogenous leukemia in blast crisis. The effects of crude pre-irradiated solutions of 5-methoxypsoralen on erythroid differentiation of human leukemic K-562 cells were evaluated. The major photoproduct was characterized and analyzed, and it was found to induce erythroid differentiation of K562 cells and inhibit NF-kappaB/DNA interactions.
Salvador
Alessia
A
Department of Pharmaceutical Sciences, University of Padova, Via Marzolo, 5, 35151, Padova, Italy.
Dall'Acqua
Stefano
S
Sardo
Manlio Sutera
MS
Caffieri
Sergio
S
Vedaldi
Daniela
D
Dall'Acqua
Francesco
F
Borgatti
Monica
M
Zuccato
Cristina
C
Bianchi
Nicoletta
N
Gambari
Roberto
R
D000078223:5-Methoxypsoralen; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D002454:Cell Differentiation; D041905:Erythroid Cells / Q000166:cytology* / Q000187:drug effects; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D007536:Isomerism; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D008968:Molecular Conformation; D016328:NF-kappa B / Q000378:metabolism; D010782:Photolysis; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D006576:Heterocyclic Compounds, 4 or More Rings; D016328:NF-kappa B; D000078223:5-Methoxypsoralen; D008730:Methoxsalen