title: [Analysis of mtDNA 12SrRNA A1555G mutations of Uigur patients with nonsyndromic hereditary hearing loss in Xinjiang].
abstract: OBJECTIVE
  To explore the incidence of Uigur patients with nonsyndromic hereditary hearing loss in Xinjiang, and to provide the basis for preventing deafness caused by aminoglycoside antibiotics.
  
  
  METHODS
  The medical history of 51 Uigur deaf patients as the study group was collected in Xinjiang. Fifty-three Uigur normal people were selected as the control group in Xinjiang. Blood samples were obtained from them with informed consents. Genomic DNA was extracted from isolated leukocytes. The mitochondrial DNA fragments were amplified by polymerase chain reaction. mtDNA 12SrRNA A1555G mutation was detected using A1w26I restriction endonuclease digestion, followed by direct sequencing to identify the A1555G mutation.
  
  
  RESULTS
  The mtDNA A1555G mutation was detected in 2 Uigur patients, and both of them had used aminoglycoside antibiotics.
  
  
  CONCLUSIONS
  There is no statistically significant difference between patients and normal people in Xinjiang. The mtDNA A1555G mutation is related to aminoglycoside antibiotics-induced deafness, which can cause genetic stisceptibility to aminoglycoside antibiotics ototoxicity. The incidence of mtDNA A1555G is lower than the average level of the overall Chinese deaf population.
authors.affiliation: Department of Otolaryngology, Affiliated Chinese Medicine Xinjiang Hospital, Medical University, Urumqi, 830000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Sheng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yang
authors.forename: Huiwu
authors.initials: H
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Luo
authors.forename: Changwei
authors.initials: C
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D017630:Connexins; D004272:DNA, Mitochondrial / Q000235:genetics*; D034381:Hearing Loss / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000235:genetics; D009154:Mutation; D010375:Pedigree; D012335:RNA, Ribosomal / Q000235:genetics*; D055815:Young Adult
chemical_list: D017630:Connexins; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S