title: Importance of non-selective cation channel TRPV4 interaction with cytoskeleton and their reciprocal regulations in cultured cells.
abstract: BACKGROUND
  TRPV4 and the cellular cytoskeleton have each been reported to influence cellular mechanosensitive processes as well as the development of mechanical hyperalgesia. If and how TRPV4 interacts with the microtubule and actin cytoskeleton at a molecular and functional level is not known.
  
  
  RESULTS
  We investigated the interaction of TRPV4 with cytoskeletal components biochemically, cell biologically by observing morphological changes of DRG-neurons and DRG-neuron-derived F-11 cells, as well as functionally with calcium imaging. We find that TRPV4 physically interacts with tubulin, actin and neurofilament proteins as well as the nociceptive molecules PKCepsilon and CamKII. The C-terminus of TRPV4 is sufficient for the direct interaction with tubulin and actin, both with their soluble and their polymeric forms. Actin and tubulin compete for binding. The interaction with TRPV4 stabilizes microtubules even under depolymerizing conditions in vitro. Accordingly, in cellular systems TRPV4 colocalizes with actin and microtubules enriched structures at submembranous regions. Both expression and activation of TRPV4 induces striking morphological changes affecting lamellipodial, filopodial, growth cone, and neurite structures in non-neuronal cells, in DRG-neuron derived F11 cells, and also in IB4-positive DRG neurons. The functional interaction of TRPV4 and the cytoskeleton is mutual as Taxol, a microtubule stabilizer, reduces the Ca2+-influx via TRPV4.
  
  
  CONCLUSIONS
  TRPV4 acts as a regulator for both, the microtubule and the actin. In turn, we describe that microtubule dynamics are an important regulator of TRPV4 activity. TRPV4 forms a supra-molecular complex containing cytoskeletal proteins and regulatory kinases. Thereby it can integrate signaling of various intracellular second messengers and signaling cascades, as well as cytoskeletal dynamics. This study points out the existence of cross-talks between non-selective cation channels and cytoskeleton at multiple levels. These cross talks may help us to understand the molecular basis of the Taxol-induced neuropathic pain development commonly observed in cancer patients.
authors.affiliation: Signal Transduction in Pain and Mental Retardation, Department for Molecular Human Genetics Max Planck Institute for Molecular Genetics, Berlin, Germany. goswami@molgen.mpg.de
authors.forename: Chandan
authors.initials: C
authors.lastname: Goswami
authors.forename: Julia
authors.initials: J
authors.lastname: Kuhn
authors.forename: Paul A
authors.initials: PA
authors.lastname: Heppenstall
authors.forename: Tim
authors.initials: T
authors.lastname: Hucho
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D016900:Neurofilament Proteins / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D050916:TRPV Cation Channels / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D016900:Neurofilament Proteins; D050916:TRPV Cation Channels; D014404:Tubulin