title: Drosophila lacks C20 and C22 PUFAs.
abstract: Drosophila melanogaster has been considered a model organism for investigating human diseases and genetic pathways. Whether Drosophila is an ideal model for nutrigenomics, especially for FA metabolism, however, remains to be illustrated. The aim of this study was to examine the metabolism of C20 and C22 PUFAs in Drosophila. Analysis of FA composition revealed a complete lack of C20 and C22 PUFAs in the body tissue of larvae, pupae, and adult flies fed either a base or supplemented diet abundant in the PUFA precursors linoleic acid and α-linolenic acid. PUFA with >C20 could only be found in flies supplemented with specific FAs. Interestingly, the supplemented C22 PUFAs docosahexaenoic acid (22:6n-3) and docosatetraenoic acid (22:4n-6) were largely converted to the shorter chain C20 PUFAs eicosapentaenoic acid (20:5n-3) and arachidonic acid (20:4n-6), respectively. Furthermore, a genome sequence scan indicated that no gene encoding Δ-6/ Δ-5 desaturases, the key enzymes for the synthesis of C20/C22 PUFA, was present in Drosophila. These findings demonstrate that Drosophila lacks the capability to synthesize the biologically important C20 and C22 PUFAs, and thereby argue that Drosophila is not a valid model for the study of lipid metabolism and related diseases.
authors.affiliation: Department of Food Science & Nutrition, Zhejiang University, Hangzhou, China.
authors.forename: Li Rong
authors.initials: LR
authors.lastname: Shen
authors.forename: Chao Qiang
authors.initials: CQ
authors.lastname: Lai
authors.forename: Xiang
authors.initials: X
authors.lastname: Feng
authors.forename: Laurence D
authors.initials: LD
authors.lastname: Parnell
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Wan
authors.forename: Jing D
authors.initials: JD
authors.lastname: Wang
authors.forename: Duo
authors.initials: D
authors.lastname: Li
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.forename: Jing X
authors.initials: JX
authors.lastname: Kang
mesh_terms: D000818:Animals; D004330:Drosophila / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases