title: Accumulation of selenium and changes in the activity of inulinase and catalase in the cells of Kluyveromyces marxianus on pulsed electric field treatment.
abstract: Pulsed electric field (PEF) of 1Hz, 1.5 kV, and 1 ms increased the activities of catalase and inulinase over the whole range of applied Se concentrations compared with the non-treated cultures. A significant effect of selenium concentration (in the range of 5-14 microg/ml) on both intra- and extracellular enzyme activities was noted. At a Se concentration of 10 microg/ml, the activities of intra- and extracellular inulinases and extracellular catalase in the PEF-treated cultures reached the maximum of 71 U/g d.m., 46 U/g d.m., and approx. 8 U/ml, respectively. The maximum activity of intracellular catalase of approx. 6 U/ ml (with and without PEF) was recorded at 5 microg Se/ml. Further increasing of selenium concentration caused a decrease in the activity of the enzymes.
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, Lublin, Poland. urszula.pankiewicz@up.lublin.pl
authors.forename: Urszula
authors.initials: U
authors.lastname: Pankiewicz
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jamroz
mesh_terms: D002374:Catalase / Q000032:analysis / Q000378:metabolism*; D018274:Electroporation; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007716:Kluyveromyces / Q000201:enzymology / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*
chemical_list: D002374:Catalase; D006026:Glycoside Hydrolases; C023086:inulinase; D012643:Selenium