Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach.
A heterotrophic, gram-negative, prosthecate bacterium, designated strain G5(T), was isolated from a sandy beach of Taean in South Korea. Cells of strain G5(T) were aerobic, catalase- and oxidase-positive, straight to slightly curved motile rods with a single flagellum and formed yellow-orange colonies on agar. Growth occurred at 15-40 °C (optimum 25-30 °C) and pH 6-9 (optimum pH 7-8). The major cellular fatty acids were C(18 : 1)ω7c, C(17 : 0), C(16 : 0), 11-methyl C(18 : 1)ω7c, C(17 : 1)ω8c and C(17 : 1)ω6c. The polar lipid pattern indicated the presence of phosphatidylglycerol, monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids. The G+C content of the genomic DNA was 63.6 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analysis showed that strain G5(T) belonged to the branch containing the genera Hellea, Robiginitomaculum and Hypomonas within the family Hyphomonadaceae. Within this group, strain G5(T) was most closely related to Hellea balneolensis 26III/A02/215(T) with 95.8 % 16S rRNA gene sequence similarity. Based on its phylogenetic position and its phenotypic, chemotaxonomic and molecular properties, strain G5(T) represents a novel species of a novel genus of the family Hyphomonadaceae, for which the name Litorimonas taeanensis gen. nov., sp. nov. is proposed. The type strain is G5(T) ( = KACC 13701(T)  = DSM 22008(T)).
Jung
Ji Young
JY
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Kim
Jeong Myeong
JM
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Jin
Hyun Mi
HM
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Kim
Sun Young
SY
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Park
Woojun
W
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-701, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001493:Bathing Beaches; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D052836:Heterotrophic Processes; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D010088:Oxidoreductases; C025204:Ubiquinone Q2