title: Functional characterization and quantification of the alternative PsbA copies in Thermosynechococcus elongatus and their role in photoprotection.
abstract: The D1 protein (PsbA) of photosystem II (PSII) from Thermosynechococcus elongatus is encoded by a psbA gene family that is typical of cyanobacteria. Although the transcription of these three genes has been studied previously (Kós, P. B., Deák, Z., Cheregi, O., and Vass, I. (2008) Biochim. Biophys. Acta 1777, 74-83), the protein quantification had not been possible due to the high sequence identity between the three PsbA copies. The successful establishment of a method to quantify the PsbA proteins on the basis of reverse phase-LC-electrospray mass ionization-MS/MS (RP-LC-ESI-MS/MS) enables an accurate comparison of transcript and protein level for the first time ever. Upon high light incubation, about 70% PsbA3 could be detected, which closely corresponds to the transcript level. It was impossible to detect any PsbA2 under all tested conditions. The construction of knock-out mutants enabled for the first time a detailed characterization of both whole cells and also isolated PSII complexes. PSII complexes of the ΔpsbA1/psbA2 mutant contained only copy PsbA3, whereas only PsbA1 could be detected in PSII complexes from the ΔpsbA3 mutant. In whole cells as well as in isolated complexes, a shift of the free energy between the redox pairs in the PsbA3 complexes in comparison with PsbA1 could be detected by thermoluminescence and delayed fluorescence measurements. This change is assigned to a shift of the redox potential of pheophytin toward more positive values. Coincidentally, no differences in the Q(A)-Q(B) electron transfer could be observed in flash-induced fluorescence decay or prompt fluorescence measurements. In conclusion, PsbA3 complexes yield a better protection against photoinhibition due to a higher probability of the harmless dissipation of excess energy.
authors.affiliation: Plant Biochemistry, Ruhr-University Bochum, D-44780 Bochum, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Sander
authors.forename: Marc
authors.initials: M
authors.lastname: Nowaczyk
authors.forename: Joachim
authors.initials: J
authors.lastname: Buchta
authors.forename: Holger
authors.initials: H
authors.lastname: Dau
authors.forename: Imre
authors.initials: I
authors.lastname: Vass
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Deák
authors.forename: Márta
authors.initials: M
authors.lastname: Dorogi
authors.forename: Masako
authors.initials: M
authors.lastname: Iwai
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport / Q000502:physiology; D005453:Fluorescence; D018628:Gene Dosage; D055785:Gene Knockdown Techniques; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; C422369:photosystem II, psbA subunit