Influence of a time-restricted feeding schedule on the daily rhythm of abcb1a gene expression and its function in rat intestine.
P-glycoprotein (P-gp) is one of the ATP-binding cassette transporters and acts as an efflux pump for cytotoxic substances. P-gp mRNA expression and transporting activity show the daily rhythm and contribute to the chrono-pharmacokinetic profiles of many drugs. It is reported that the daily rhythm of abcb1a mRNA is regulated by a circadian clock-controlled output pathway. Time-restricted feeding is well known to shift the peripheral circadian phase of clock gene expression without changing the central clock function. This study was undertaken to examine the influence of a time-restricted feeding procedure during the light phase on the daily rhythms of abcb1a mRNA expression and P-gp activity. The abcb1a mRNA and P-gp activity showed a daily rhythm with a peak early in the dark phase in rat intestine under ad libitum feeding. Time-restricted feeding during the light phase shifted these rhythms to 12-h advance. The mRNA expression of clock genes (DBP and HLF, the transcript activators of abcb1a) also showed daily rhythms, and their phases were shifted by the time-restricted feeding procedure. The peak time of DBP mRNA expression was similar to that of abcb1a mRNA expression under ad libitum feeding and time-restricted feeding conditions. These results indicate that a time-restricted feeding procedure changes DBP mRNA expression, which in turn influences abcb1a mRNA expression and P-gp activity.
Hayashi
Yohei
Y
Division of Clinical Pharmacology, Department of Pharmacology, Jichi Medical University, Tochigi 329-0498.
Ushijima
Kentarou
K
Ando
Hitoshi
H
Yanagihara
Hayato
H
Ishikawa
Eiko
E
Tsuruoka
Shu-Ichi
S
Sugimoto
Ko-Ichi
K
Fujimura
Akio
A
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001692:Biological Transport; D002940:Circadian Rhythm / Q000235:genetics*; D003345:Corticosterone / Q000097:blood; D004077:Digoxin / Q000493:pharmacokinetics; D004435:Eating / Q000235:genetics*; D015870:Gene Expression; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity
D018435:ATP Binding Cassette Transporter, Subfamily B; D012333:RNA, Messenger; D004077:Digoxin; C095122:multidrug resistance protein 3; D003345:Corticosterone