Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection.
To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH(2)) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH(2) and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH(2) /DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.
Lim
Eun-Kyung
EK
Dept. of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Korea.
Yang
Jaemoon
J
Suh
Jin-Suck
JS
Huh
Yong-Min
YM
Haam
Seungjoo
S
D000586:Amination; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D015394:Molecular Structure; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry; D011136:Polysorbates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014162:Transfection
D000970:Antineoplastic Agents; D004337:Drug Carriers; D011136:Polysorbates; D049452:Green Fluorescent Proteins; D004247:DNA