CXCR4, a potential predictive marker for docetaxel sensitivity in gastric cancer.
The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer.


METHODS
Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. A panel of 11 genes selected according to microarray analysis were validated and tested further in 11 cancer cell lines, resulting in 4 genes, CXCR4, CDK6, USP15 and CDH1. Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens.


RESULTS
Only CXCR4 mRNA levels in gastric cancer tissues were correlated with docetaxel sensitivity (R(2)=0.23, p=0.019) and significantly higher in resistant specimens (p=0.038). AMD3100, a CXCR4 antagonist, enhanced the docetaxel cytotoxicity in vitro.


CONCLUSIONS
CXCR4 mRNA expression levels may be a potential predictive biomarker in gastric cancer.
Xie
Li
L
The Comprehensive Cancer Center, of Drum Tower Hospital, affiliated to the Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, PR China.
Wei
Jia
J
Qian
Xiaoping
X
Chen
Gang
G
Yu
Lixia
L
Ding
Yitao
Y
Liu
Baorui
B
D001596:Benzylamines; D015415:Biomarkers; D045744:Cell Line, Tumor; D000080027:Cyclams; D000077143:Docetaxel; D019008:Drug Resistance, Neoplasm; D020869:Gene Expression Profiling; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D043823:Taxoids / Q000494:pharmacology*
D001596:Benzylamines; D015415:Biomarkers; C527276:CXCR4 protein, human; D000080027:Cyclams; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; D043823:Taxoids; D000077143:Docetaxel; C088327:plerixafor