A graphene-enhanced molecular beacon for homogeneous DNA detection.
In this work, we report the design of a novel graphene-based molecular beacon (MB) that could sensitively and selectively detect specific DNA sequences. The ability of water-soluble graphene oxide (GO) to differentiated hairpin and dsDNA offered a new approach to detect DNA. We found that the background fluorescence of MB was significantly suppressed in the presence of GO, which increased the signal-to-background ratio, hence the sensitivity. Moreover, the single-mismatch differentiation ability of hairpin DNA was maintained, leading to high selectivity of this new method.
Li
Fan
F
Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China.
Huang
Yan
Y
Yang
Qing
Q
Zhong
Zentao
Z
Li
Di
D
Wang
Lihua
L
Song
Shiping
S
Fan
Chunhai
C
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000737:chemistry; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D010087:Oxides; D002244:Carbon; D004247:DNA