title: Determinants of the proinflammatory action of ambient particulate matter in immortalized murine macrophages.
abstract: BACKGROUND
  Proximity to traffic-related pollution has been associated with poor respiratory health in adults and children.
  
  
  OBJECTIVE
  We wished to test the hypothesis that particulate matter (PM) from high-traffic sites would display an enhanced capacity to elicit inflammation.
  
  
  METHODS
  We examined the inflammatory potential of coarse [2.5-10 µm in aerodynamic diameter (PM(2.5-10))] and fine [0.1-2.5 µm in aerodynamic diameter (PM(0.1-2.5))] PM collected from nine sites throughout Europe with contrasting traffic contributions. We incubated murine monocytic-macrophagic RAW264.7 cells with PM samples from these sites (20 or 60 µg/cm²) and quantified their capacity to stimulate the release of arachidonic acid (AA) or the production of interleukin-6 and tumor necrosis factor-α (TNFα) as measures of their inflammatory potential. Responses were then related to PM composition: metals, hydrocarbons, anions/cations, and endotoxin content.
  
  
  RESULTS
  Inflammatory responses to ambient PM varied markedly on an equal mass basis, with PM(2.5-10) displaying the largest signals and contrasts among sites. Notably, we found no evidence of enhanced inflammatory potential at high-traffic sites and observed some of the largest responses at sites distant from traffic. Correlation analyses indicated that much of the sample-to-sample contrast in the proinflammatory response was related to the content of endotoxin and transition metals (especially iron and copper) in PM(2.5-10). Use of the metal chelator diethylene triamine pentaacetic acid inhibited AA release, whereas recombinant endotoxin-neutralizing protein partially inhibited TNFα production, demonstrating that different PM components triggered inflammatory responses through separate pathways.
  
  
  CONCLUSIONS
  We found no evidence that PM collected from sites in close proximity to traffic sources displayed enhanced proinflammatory activity in RAW264.7 cells.
authors.affiliation: Department of Environment and Primary Prevention, Instituto Superiore di Sanita, Rome, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Guastadisegni
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Kelly
authors.forename: Flemming R
authors.initials: FR
authors.lastname: Cassee
authors.forename: Miriam E
authors.initials: ME
authors.lastname: Gerlofs-Nijland
authors.forename: Nicole A H
authors.initials: NA
authors.lastname: Janssen
authors.forename: Roberta
authors.initials: R
authors.lastname: Pozzi
authors.forename: Bert
authors.initials: B
authors.lastname: Brunekreef
authors.forename: Thomas
authors.initials: T
authors.lastname: Sandström
authors.forename: Ian
authors.initials: I
authors.lastname: Mudway
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D004369:Pentetic Acid / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D018836:Inflammation Mediators; D052638:Particulate Matter; D014409:Tumor Necrosis Factor-alpha; D016718:Arachidonic Acid; D004369:Pentetic Acid