Enzymatic and mRNA transcript response of ovine 6-phosphogluconate dehydrogenase (6PGD) in respect to different weights from weaning to four months of age.
Ovine 6-phosphogluconate dehydrogenase (6PGD), an enzyme of the pentose phosphate pathway, provides the necessary compounds of NADPH for the synthesis of fatty acids. Much of research has been conducted not only on the enzymatic level, but also on molecular level elucidating its cDNA sequence. Herein, we tried to elucidate if any correlation between enzymatic activity and expression of ovine 6PGD gene exists, in respect to two different weights from weaning to 4 months old. 18 male and 16 female lambs of Chios breed were randomly selected after weaning and assigned to two groups based on sex in a different experimental open-plan shed. Two subgroups were defined in each sex and they were slaughtered at 25 kg and 30 kg, respectively. Samples of adipose tissue (tail, perirenal and shoulder site) were collected and 6PGD enzymatic activity, gene expression, and characteristics of adipocytes were determined. According to the determined data, tail subcutaneous adipose tissue matures later than the others examined tissues and has a diminished lipogenic activity. A 6PGD gene expression pattern was not followed by analogous changes of its enzymatic activity, suggesting that other mechanisms such as post transcription or/and post translation regulation may be involved.
Laliotis
George P
GP
Department of Animal Science, Agricultural University of Athens, Greece.
Trivizaki
Stamatina
S
Bizelis
Iosif
I
Charismiadou
Maria A
MA
Rogdakis
Emmanuel
E
D017667:Adipocytes / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D001835:Body Weight / Q000502:physiology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D016018:Least-Squares Analysis; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology / Q000201:enzymology*
D012333:RNA, Messenger; D010734:Phosphogluconate Dehydrogenase