[In vivo experimental study on nasopharyngeal carcinoma with combination of pAdKDR-tk/GCV suicide gene therapy system and 60Co radiotherapy].
OBJECTIVE
To explore the killing effect of pAdKDR-tk/GCV suicide gene therapy system combined with 60Co radiotherapy on human nasopharyngeal carcinoma in vivo.


METHODS
The pAdKDR-tk/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination for human nasopharyngeal carcinoma in vivo to compare their effects. The tumor growth curve and inhibition rate of tumor to the cure effects of the combination of the pAdKDR-tk/GCV suicide gene therapy system and 60Co radiotherapy.


RESULTS
The inhibition rate of gene therapy alone and radiotherapy alone in curing the transplanted tumor in nude mouse subcutaneously was 58.43% and 70.88% respectively, and the combined application of gene therapy and radiotherapy exhibited stronger therapeutic effects (the inhibition rate was 84.39%, P<0.01), the mean tumor volume of the combination group was only 13.5% of the mean tumor volume of compared group, and it was obviously lower than the mean tumor volume of gene therapy alone and radiotherapy alone in the twenty-first day.


CONCLUSIONS
The combined application of gene therapy and radiotherapy has an obviously higher curative effect than simple therapy. This method would establish a theoretic and clinical basis for the research of combination of suicide gene system tumor vascular targeting treating and radiotherapy.
Qiu
Qianhui
Q
Department of Otorhinolaryngology-Head and Neck Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China. qiuqianhui@hotmail.com
Sun
Wei
W
Chen
Shaohua
S
Huang
Xiaoming
X
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000532:radiotherapy / Q000628:therapy*; D018139:Simplexvirus / Q000235:genetics; D014162:Transfection
D015774:Ganciclovir