Compounds from Viburnum sargentii Koehne and evaluation of their cytotoxic effects on human cancer cell lines.
Compounds were isolated from a methanol extract of the dried stem barks of Viburnum sargentii Koehne. The structures of the compounds, namely 9'-O-methylvibsanol (3), furcatoside A (4) and lareciresinol (5) were elucidated by analysis of spectroscopic data and comparison with values for previously known analogues. In addition, (+)-catechin (1), (+)-epicatechin (2) were also isolated. This work also examined the cytotoxic effects of three compounds 3-5 (25-100 microM) in MCF-7 and A549 cells after 24, 48 and 72 h of exposure. Our results showed that 9'-O-methylvibsanol (3) exhibited strong concentration-dependent anticancer effects according to the MTT assay and produced morphological changes consistent with apoptosis, as confirmed by Ho3342 staining analysis revealed that more apoptotic cells were observed after 9'-Omethylvibsanol (3) treatment.
Bae
Ki-Eun
KE
Department of Forest Products and Biotechnology, College of Forest Sciences, Kookmin University, Seoul, 136-702, Korea. kieun@kookmin.ac.kr
Chong
Han-Soo
HS
Kim
Dong-Sup
DS
Choi
Young-Woong
YW
Kim
Young-Sook
YS
Kim
Young-Kyoon
YK
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D013057:Spectrum Analysis; D013997:Time Factors; D027925:Viburnum / Q000737:chemistry*
D000970:Antineoplastic Agents; D010936:Plant Extracts