title: Functional impact of transposable elements using bioinformatic analysis and a comparative genomic approach.
abstract: A dual coding event, which is the translation of different isoforms from a single gene, is one of the special patterns among the alternative splicing events. This is an important mechanism for the regulation of protein diversity in human and mouse genomes. Although the regulation for dual coding events has been characterized in a few genes, the individual mechanism remains unclear. Numerous studies have described the exonization of transposable elements, which is the splicing mediated insertion of transposable element sequence fragments into mature mRNAs. Therefore, in this study, we investigated the number of transposable element (TE)-derived dual coding genes in human, chimpanzee and mouse genomes. TE fusion exons appeared in the dual coding regions of 309 human genes. Functional protein domain alterations by TE-derived dual coding events were observed in 129 human genes. Comparative TE-derived dual coding events were also analyzed in chimpanzee and mouse orthologs. Seventy chimpanzee orthologs had TE-derived dual coding events, but mouse orthologs did not have any TE-derived dual coding events. Taken together, our analyses listed the number of TE-derived dual coding genes which could be investigated by experimental analysis and suggested that TE-derived dual coding events were major sources for the functional diversity of human genes, but not mouse genes.
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 363-883, Korea.
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Huh
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Kim
authors.forename: Sang-Je
authors.initials: SJ
authors.lastname: Park
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D004251:DNA Transposable Elements; D005091:Exons; D016678:Genome; D023281:Genomics / Q000379:methods*; D006801:Humans; D051379:Mice; D002679:Pan troglodytes
chemical_list: D004251:DNA Transposable Elements