Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1.
In plant innate immunity, the leucine-rich repeat receptor kinase FLS2 recognizes the bacterial pathogen-associated molecular pattern (PAMP) flagellin. The molecular mechanisms underlying PAMP perception are not fully understood. Here, we reveal that the gaseous phytohormone ethylene is an integral part of PAMP-triggered immunity. Plants mutated in the key ethylene-signaling protein EIN2 are impaired in all FLS2-mediated responses, correlating with reduced FLS2 transcription and protein accumulation. The EIN3 and EIN3-like transcription factors, which depend on EIN2 activity for their accumulation, directly control FLS2 expression. Our results reveal a direct role for ethylene in regulation of an innate immune receptor.
Boutrot
Freddy
F
The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.
Segonzac
Cécile
C
Chang
Katherine N
KN
Qiao
Hong
H
Ecker
Joseph R
JR
Zipfel
Cyril
C
Rathjen
John P
JP
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics*; D004268:DNA-Binding Proteins; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics*; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C494589:EIL1 protein, Arabidopsis; C106987:EIN3 protein, Arabidopsis; D005030:Ethylenes; D009687:Nuclear Proteins; D011971:Receptors, Immunologic; D014157:Transcription Factors; C036216:ethylene; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis