title: Aromatic acid metabolites of Escherichia coli K-12 can induce the marRAB operon.
abstract: MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We also found that a third intermediate involved in ubiquinone biosynthesis, 4-hydroxybenzoate, activates marRAB transcription in the absence of TolC. Of the three, however, only 2,3-dihydroxybenzoate directly binds MarR and affects its activity.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Lon M
authors.initials: LM
authors.lastname: Chubiz
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Rao
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D062385:Hydroxybenzoates / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics; D009876:Operon / Q000187:drug effects* / Q000235:genetics; D010226:Parabens / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D062385:Hydroxybenzoates; C080002:MarA protein, E coli; C080004:MarR protein, E coli; D026901:Membrane Transport Proteins; D010226:Parabens; D012097:Repressor Proteins; C080003:multiple antibiotic resistance protein B; D062367:ortho-Aminobenzoates; C073126:tolC protein, E coli; C031385:anthranilic acid; C009135:2,3-dihydroxybenzoic acid; C038193:4-hydroxybenzoic acid