title: In vitro and in vivo effects of Laurus nobilis L. leaf extracts.
abstract: The in vitro and in vivo antioxidant activities of different extracts of laurel leaves were studied. Free radical scavenging capacity (RSC) was evaluated measuring the scavenging activity on the DPPH, NO, O(2)(.-) and OH radicals. The effects on lipid peroxidation (LP) were also evaluated. Experimental results indicate that ethyl acetate extract of leaves has exhibited the largest RSC capacity in neutralization of DPPH, NO, O(2)(.-) and OH radicals. The same result was obtained in investigation of extracts impact on LP. The in vivo effects were evaluated on some antioxidant systems (activities of GSHPx, LPx, Px, CAT and XOD, and GSH content) in the mice liver and blood-hemolysate after treatment with the examined laurel extracts, or in combination with carbon tetrachloride (CCl(4)). On the basis of the results obtained it can be concluded that the examined extracts exhibited a certain protective effect, which is more pronounced on the liver than on blood-hemolysate parameters. The results obtained indicate toxicity of CCl(4), probably due to the radicals involved in its metabolism. Combined treatments with CCl(4) and the examined extracts showed both positive and negative synergism. Based on the experimental results, the strongest protective effect was shown by the EtOAc extract.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia. biljana.kaurinovic@dh.uns.ac.rs
authors.forename: Biljana
authors.initials: B
authors.lastname: Kaurinovic
authors.forename: Mira
authors.initials: M
authors.lastname: Popovic
authors.forename: Sanja
authors.initials: S
authors.lastname: Vlaisavljevic
mesh_terms: D000085:Acetates; D000818:Animals; D002251:Carbon Tetrachloride; D016166:Free Radical Scavengers; D006461:Hemolysis / Q000187:drug effects; D027442:Laurus / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D051379:Mice; D016877:Oxidants; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000085:Acetates; D016166:Free Radical Scavengers; D016877:Oxidants; D010936:Plant Extracts; C007650:ethyl acetate; D002251:Carbon Tetrachloride