Evaluation of immune response and resistance to diseases in tiger shrimp, Penaeus monodon fed with biofilm of Vibrio alginolyticus.
Immune response in juvenile tiger shrimp, Penaeus monodon fed with biofilm (BF) and free cells (FC) of Vibrio alginolyticus was studied by evaluating the hemocyte count, phenoloxidase activity and antibacterial activity. The above immune responses were higher in BF fed shrimp than that in FC fed or control shrimp. Among the different doses of BF of V. alginolyticus tested, 10(9) cfu g(-1) shrimp day(-1) for two weeks could evoke higher immune response. BF fed shrimp were more resistant to injection challenge with V. alginolyticus and whitespot syndrome virus (WSSV) with significantly higher RPS compared to that with FC fed and control shrimp. Better resistance was also reflected by rapid clearance of V. alginolyticus and WSSV from the hemolymph as confirmed by immunodot and histopathology.
Sharma
S R Krupesha
SR
Fish Pathology & Biotechnology Laboratory, Department of Aquaculture, College of Fisheries, Mangalore 575002, India.
Shankar
K M
KM
Sathyanarayana
M L
ML
Sahoo
A K
AK
Patil
Rajreddy
R
Narayanaswamy
H D
HD
Rao
Suguna
S
D000704:Analysis of Variance; D000818:Animals; D018441:Biofilms; D001772:Blood Cell Count; D004032:Diet; D006434:Hemocytes; D007113:Immunity, Innate / Q000276:immunology*; D007194:India; D007928:Lethal Dose 50; D014442:Monophenol Monooxygenase / Q000378:metabolism; D046849:Nimaviridae / Q000276:immunology*; D033561:Penaeidae / Q000276:immunology*; D044149:Vibrio alginolyticus / Q000276:immunology*
D014442:Monophenol Monooxygenase