title: Proteomic study of Carissa spinarum in response to combined heat and drought stress.
abstract: Carissa spinarum is one of the secondary advantage plants grown in dry-hot valleys in China, which can survive under stress conditions of high temperature and extreme low humidity. Here, we studied the physiological and proteomic changes of C. spinarum in response to 42 degrees C heat stress treatment in combination with drought stress. Dynamic changes in the leaf proteome were analyzed at four time points during the stress treatment and recovery stages. Approximately, 650 protein spots were reproducibly detected in each gel. Forty-nine spots changed their expression levels upon heat and drought treatment, and 30 proteins were identified by MS and 2-D Western blot. These proteins were classified into several categories including HSP, photosynthesis-related protein, RNA-processing protein and proteins involved in metabolism and energy production. The potential roles of these stress-responsive proteins are discussed.
authors.affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, PR China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Zhang
authors.forename: Guowei
authors.initials: G
authors.lastname: Li
authors.forename: Gwowei
authors.initials: G
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Ting
authors.initials: T
authors.lastname: Bi
authors.forename: Genyun
authors.initials: G
authors.lastname: Chen
authors.forename: Zhangcheng
authors.initials: Z
authors.lastname: Tang
authors.forename: Weiai
authors.initials: W
authors.lastname: Su
authors.forename: Weining
authors.initials: W
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D029062:Apocynaceae / Q000378:metabolism / Q000502:physiology*; D018833:Chaperonins / Q000737:chemistry / Q000378:metabolism; D016000:Cluster Analysis; D055864:Droughts; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D020543:Proteome; D018833:Chaperonins