Detection of Saint Louis encephalitis virus in dengue-suspected cases during a dengue 3 outbreak.
Arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem. Among the Brazilian arboviruses, Mayaro virus, Dengue virus (DENV), Yellow Fever virus, Rocio virus, Saint Louis Encephalitis virus (SLEV), and Oropouche virus are responsible for most of human cases. All these arboviruses usually produce undistinguishable acute febrile illness, especially in the acute phase of infection. In this study we investigated the presence of arboviruses in sera of 519 patients presenting acute febrile illness, during a dengue outbreak in São José do Rio Preto City (São Paulo, Brazil). A multiplex-nested RT-polymerase chain reaction assay was applied to detect and identify the main Brazilian arboviruses (Flavivirus, Alphavirus, and Orthobunyavirus genera). The molecular analysis showed that 365 samples were positive to DENV-3, 5 to DENV-2, and 8 to SLEV. Among the positive samples, one coinfection was detected between DENV-2 and DENV-3. The phylogenetic analysis of the SLEV envelope gene indicated that the virus circulating in city is related to lineage V strains. These results indicated that during that large DENV-3 outbreak in 2006, different arboviruses cocirculated causing human disease. Thus, it is necessary to have an efficient surveillance system to control the dissemination of these arboviruses in the population.
Terzian
Ana Carolina Bernardes
AC
Laboratório de Pesquisa em Virologia, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, São Paulo, Brazil.
Mondini
Adriano
A
Bronzoni
Roberta Vieira de Moraes
RV
Drumond
Betânia Paiva
BP
Ferro
Bianca Piovezan
BP
Cabrera
Eliana Márcia Sotello
EM
Figueiredo
Luis Tadeu Moraes
LT
Chiaravalloti-Neto
Francisco
F
Nogueira
Maurício Lacerda
ML
D000330:Aedes / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D001103:Arboviruses / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002460:Cell Line; D060085:Coinfection; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics* / Q000302:isolation & purification; D004196:Disease Outbreaks; D004665:Encephalitis Virus, St. Louis / Q000235:genetics* / Q000302:isolation & purification; D004674:Encephalitis, St. Louis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction; D010802:Phylogeny; D011634:Public Health; D012367:RNA, Viral / Q000097:blood*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
D012367:RNA, Viral; D014759:Viral Envelope Proteins