A clear correlation between WT1-specific Th response and clinical response in WT1 CTL epitope vaccination.
Clinical studies of WT1-targeted cancer vaccine are being performed. However, WT1-specific Th response in cancer patients remains unclear. Using quantitative real-time RT-PCR, we investigated IFN-gamma and IL-10 mRNA expression from Th cells by stimulation with helper peptide WT1(332). Seventeen patients, of whom 10 had achieved stable disease and the remaining 7 had progressive disease, were weekly vaccinated with WT1 CTL epitope (modified WT1(235)) and examined for WT1(332)-specific Th response. A clear correlation between WT1(332)-specific Th response and clinical response was observed at 4 weeks post-vaccination. In patients who responded, a clear inverse correlation between IL-10-type and IFN-gamma-type WT1(332)-specific Th response was detected at pre- and 4 weeks post-vaccination, and the shift of the Th response from IL-10-type dominancy at early phase to IFN-gamma-type dominancy at late phase was observed. From this study we concluded that occurrence of WT1(332)-specific Th response could predict good clinical response of WT1 CTL epitope vaccination.
Fujiki
Fumihiro
F
Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, 1-7, Yamada-Oka, Suita City, Osaka 565-0871, Japan.
Oka
Yoshihiro
Y
Kawakatsu
Mai
M
Tsuboi
Akihiro
A
Tanaka-Harada
Yukie
Y
Hosen
Naoki
N
Nishida
Sumiyuki
S
Shirakata
Toshiaki
T
Nakajima
Hiroko
H
Tatsumi
Naoya
N
Hashimoto
Naoya
N
Taguchi
Tetsuya
T
Ueda
Satsuki
S
Nonomura
Norio
N
Takeda
Yutaka
Y
Ito
Toshinori
T
Myoui
Akira
A
Izumoto
Shuichi
S
Maruno
Motohiko
M
Yoshimine
Toshiki
T
Noguchi
Shinzaburo
S
Okuyama
Akihiko
A
Kawase
Ichiro
I
Oji
Yusuke
Y
Sugiyama
Haruo
H
D000328:Adult; D000368:Aged; D019496:Cancer Vaccines / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014611:Vaccination; D025721:WT1 Proteins / Q000276:immunology*
D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D012333:RNA, Messenger; D025721:WT1 Proteins; D016753:Interleukin-10; D007371:Interferon-gamma