Quantitative analysis and toxicity determination of artifacts originated in a Thai traditional medicine Prasaplai.
Prasaplai is a Thai traditional medicine for relieving dysmenorrhea and adjusting the menstrual cycle. Three fatty acid esters, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-yl linoleate (1), (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-yl oleate (2) and (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-yl palmitate (3) are formed during storage from the reaction of chemical components in two herbs, i.e., fatty acids in Nigella sativa (L.) (Ranunculaceae) and (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (compound D) in Zingiber cassumunar (Roxb.) (Zingiberaceae). The formations of these artifacts were monitored for 1 year and their amounts were analyzed by HPLC at certain periods of time. The results showed that artifact formation was saturated after 73 days of storage. The amount of each artifact in the saturation period ranged from 3.93 +/- 0.06 to 4.30 +/- 0.18% w/w for compound 1, 1.69 +/- 0.08 to 1.9 +/- 0.13% w/w for compound 2 and 0.09 +/- 0.003 to 0.1 +/- 0.005% w/w for compound 3. Cytotoxicity of the artifacts was evaluated using NCI-H187, KB, and BC cancer cell lines and found that the IC(50) of all artifacts in all tests were higher than 20 microg/mL. For acute toxicity in mice, the LD(50) of each artifact was more than 300 mg/kg.
Tangyuenyongwatana
Prasan
P
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Gritsanapan
Wandee
W
D000818:Animals; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D031881:Nigella sativa; D010936:Plant Extracts / Q000302:isolation & purification* / Q000633:toxicity*; D027343:Rhizome; D012639:Seeds; D013785:Thailand; D030521:Zingiberaceae
D010936:Plant Extracts