Dendritic cells continue to capture and present antigens after maturation in vivo.
Dendritic cell (DC) maturation is critical for the regulation of T cell responses. The downregulation of endocytosis on maturation is considered a key adaptation that dissociates prior Ag capture by DCs from subsequent T cell engagement. To study the dynamics of Ag capture and presentation in situ, we studied the capacity for Ag uptake by DCs matured in their natural tissue environment. We found that after maturation in vivo, mouse DCs retained a robust capacity to capture soluble Ags. Furthermore, Ags internalized by mature DCs were efficiently presented on MHC class II and cross-presented on MHC class I. These results suggest that under inflammatory conditions, mature DCs may contribute to T cell stimulation without exclusively relying on prior exposure to Ags as immature DC precursors.
Drutman
Scott B
SB
Cancer Institute, New York University School of Medicine, New York, NY 10016, USA.
Trombetta
E Sergio
ES
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004705:Endocytosis / Q000276:immunology; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology
D000941:Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D010047:Ovalbumin