title: Effects of Alchornea cordifolia on elastase and superoxide anion produced by human neutrophils.
abstract: The ability of Alchornea cordifolia (Schum. and Thonn.) Müll. Arg. (Euphorbiaceae) leaves to inhibit human neutrophil elastase (HNE) and superoxide anion (O(2)(*-)) activities was evaluated on aqueous and ethyl acetate extracts as they allow for a targeted extraction of polyphenols. The direct effect of A. cordifolia extracts on HNE and O(2)(*-) was assessed in an acellular system. Results showed that extracts scavenge HNE and O(2)(*-) in a dose-dependent manner. Better activity was exhibited by the ethyl acetate extract with lower IC(50) (2.2 and 4. 1 mg/L for HNE and O(2)(*-), respectively) than for the aqueous extract. Cellular systems including isolated human polymorphonuclear neutrophils (PMN) were investigated to assess the effect of extracts on PMN metabolism. PMN were stimulated with 4beta-phorbol-12-myristate-13-acetate (PMA), calcium ionophore (CaI), or N-formyl-methionyl-leucine-phenylalanine (fMLP), each stimulant having its own stimulation pathway. From the IC(50) obtained, it can be concluded that A. cordifolia reduces HNE and O(2)(*-) liberation. Furthermore it was demonstrated that A. cordifolia extracts have no cytotoxic activity on PMN by measuring release of the cytosolic enzyme lactate dehydrogenase. As the ethyl acetate extract offers a higher rate of total phenols than the aqueous extract as well as better scavenging activity, it can be supposed that polyphenols, which are well known for their potent antioxidant and antielastase activity, are implicated in the activity of the plant. Phenolic substances such as quercetin, myricetin-3-glucopyranoside, myricetin-3-rhamnopyranoside, and proanthocyanidin A2 were identified in the ethyl acetate extract. In conclusion, the study provides proof of ethnomedical claims and partly explains the mechanisms of the anti-inflammatory action of A. cordifolia leaves.
authors.affiliation: Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, Lille, France.
authors.forename: Gisèle
authors.initials: G
authors.lastname: Kouakou-Siransy
authors.forename: Sevser
authors.initials: S
authors.lastname: Sahpaz
authors.forename: G Irié
authors.initials: GI
authors.lastname: Nguessan
authors.forename: Jacques Yao
authors.initials: JY
authors.lastname: Datté
authors.forename: Jérome Kablan
authors.initials: JK
authors.lastname: Brou
authors.forename: Bernard
authors.initials: B
authors.lastname: Gressier
authors.forename: François
authors.initials: F
authors.lastname: Bailleul
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D019665:Euphorbiaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D019272:Leukocyte Elastase / Q000378:metabolism*; D016488:Medicine, African Traditional; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols; D012995:Solubility; D013481:Superoxides / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013481:Superoxides; D019272:Leukocyte Elastase