Comparative transmembrane transports of four typical lipophilic organic chemicals.
Transmembrane transports of four kinds of lipophilic organic chemicals (LOCs) on suspending multilamellar liposomes (SML) and Escherichia coli (E. coli) were investigated, where both anthracene and phenanthrene were accorded to the lipid-water partition law and Sudan I and III to the Langmuir isothermal adsorption. Less than half of phenanthrene is transported into E. coli, where more than 60% are located in the cytoplasm. About 60% of anthracene entered the E. coli where only 10% was released into the cytoplasm. The partition coefficients of phenanthrene and anthracene partitioning from the extracellular liquid into membrane are 502 and 1190L/kg but their inverse partition coefficients are only 0.180 and 0.018kg/L. Over 60% of Sudan I and less than 40% of Sudan III accumulated on E. coli where most of them remained on the membrane. The transmembrane impedance effect (TMIE) is proposed for evaluating the cell-transport of polar LOCs.
Ren
Jiao-Rong
JR
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, China.
Zhao
He-Ping
HP
Song
Chao
C
Wang
Shi-Long
SL
Li
Lu
L
Xu
Ya-Tong
YT
Gao
Hong-Wen
HW
D001693:Biological Transport, Active / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D054709:Lecithins / Q000493:pharmacokinetics*; D008081:Liposomes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics*
D054709:Lecithins; D008081:Liposomes; D011084:Polycyclic Aromatic Hydrocarbons