title: Increased levels of IgA antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi differentiate digestive forms of Chagas disease.
abstract: In the chronic phase of Chagas disease, individuals infected by Trypanosoma cruzi may be asymptomatic or may present cardiac and/or digestive complications. Our aim here was to analyze the relationship between the presence of specific immunoglobulin A antibodies and the different chronic clinical forms of Chagas disease using two recombinant antigens of Trypanosoma cruzi, cytoplasmatic repetitive antigen and flagellar repetitive antigen. The association of this immunoglobulin isotype with the digestive and cardio-digestive forms of the disease determined by indirect enzyme-linked immunosorbent assay, strongly suggests that IgA antibodies against these recombinant antigens of T. cruzi can be used as an immunological marker of the digestive alterations caused by Chagas disease. The tests performed in this study show that it is possible to differentiate digestive forms of Chagas disease. The knowledge provided by these results may help physicians to manage early alterations in the digestive tract of patients with the indeterminate or cardiac forms of Chagas disease. Prospective studies, however, with follow-up of the patients that presenting with high levels of immunoglobulin A against cytoplasmatic repetitive antigen and flagellar repetitive antigen recombinant antigens, need to be conducted to confirm this hypothesis.
authors.affiliation: Departamento de Imunologia, Instituto Aggeu Magalhães/Fiocruz, Recife, Pernambuco, Brazil.
authors.forename: Romero H T
authors.initials: RH
authors.lastname: Vasconcelos
authors.forename: Fábio N
authors.initials: FN
authors.lastname: Amaral
authors.forename: Maria G A M
authors.initials: MG
authors.lastname: Cavalcanti
authors.forename: Edimilson D
authors.initials: ED
authors.lastname: Silva
authors.forename: Antonio G P
authors.initials: AG
authors.lastname: Ferreira
authors.forename: Clarice N L
authors.initials: CN
authors.lastname: Morais
authors.forename: Yara M
authors.initials: YM
authors.lastname: Gomes
mesh_terms: D000328:Adult; D000368:Aged; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D014355:Chagas Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000503:physiopathology; D002908:Chronic Disease; D003937:Diagnosis, Differential; D004066:Digestive System Diseases; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000953:Antigens, Protozoan; D015415:Biomarkers; D007070:Immunoglobulin A; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C060967:cytoplasmic antigen, Trypanosoma; C052356:flagellum-specific antigen, Trypanosoma