title: Contribution of mevalonate and methylerythritol phosphate pathways to polyisoprenoid biosynthesis in the rubber-producing plant Eucommia ulmoides oliver.
abstract: The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the first time by feeding experiments using 13C-labeled isotopomers of (RS)-mevalonate, 1-deoxy-D-xylulose-3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13C NMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopentenyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisoprenoid; therefore, it was observed in the formation of low-molecular weight isoprenoids.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. bamba@bio.eng.osaka-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
authors.forename: Michiko
authors.initials: M
authors.lastname: Murayoshi
authors.forename: Koichirou
authors.initials: K
authors.lastname: Gyoksen
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okumoto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Katto
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
mesh_terms: D002247:Carbon Isotopes; D031283:Eucommiaceae / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008798:Mevalonic Acid / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D012408:Rubber / Q000138:chemical synthesis*; D036226:Seedlings / Q000378:metabolism; D012997:Solvents
chemical_list: D002247:Carbon Isotopes; D011106:Polyisoprenyl Phosphates; D012997:Solvents; D012408:Rubber; D008798:Mevalonic Acid