title: Solid-phase synthesis of lipidated Ras peptides employing the Ellman sulfonamide linker.
abstract: A detailed study on the solid-phase synthesis of lipidated peptides of the Ras family employing the Ellman sulfonamide linker is reported. Using the C-terminal N-Ras sequence, critical issues such as lipidated amino acid resin loading, peptide elongation in the presence of labile groups and optimized conditions for release of the peptides were investigated. A versatile methodology for the synthesis of peptides with diverse lipid motifs and C-terminal methyl esters has accordingly been established.
authors.affiliation: Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
authors.forename: Gemma
authors.initials: G
authors.lastname: Triola
authors.forename: Marc
authors.initials: M
authors.lastname: Gerauer
authors.forename: Kristina
authors.initials: K
authors.lastname: Görmer
authors.forename: Lucas
authors.initials: L
authors.lastname: Brunsveld
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000235:genetics; D013449:Sulfonamides / Q000737:chemistry*; D018631:ras Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D000596:Amino Acids; D008055:Lipids; D010455:Peptides; D013449:Sulfonamides; D018631:ras Proteins