title: The allele frequencies of HPA 1-16 determined by PCR-SSP in Chinese Cantonese donors.
abstract: BACKGROUND
  Typing of human platelet antigens (HPA) has proven to be useful in some clinical situations related to platelet alloimmunization.
  
  
  OBJECTIVE
  The objective of this study was to investigate HPA 1-16 and to determine genotype and allele frequencies by polymerase chain reaction-sequence specific primer (PCR-SSP) in apheresis platelet donors in Guangzhou Blood Center.
  
  
  METHODS
  A total of 200 random samples from donors were involved in the study. Genotype and allele frequencies of HPA 1 to 16 were detected by PCR-SSP method.
  
  
  RESULTS
  The frequencies obtained from these donors were 99·50 and 0·50% for HPA-1a and -1b; 96·25 and 3·75% for HPA-2a and -2b; 54·25 and 45·75% for HPA-3a and -3b; 99·50 and 0·50% for HPA-4a and -4b; 99·00 and 1·00% for HPA-5a and -5b; 97·00 and 3·00% for HPA-6a and -6b and 42·25 and 57·75% for HPA-15a and -15b. There is only a/a homozygosis detected in HPA-7, -8, -9, -10, -11, -12, -13, -14 and -16. In this study, none of HPA-1b/ 1b, -2b/2b, -5b/5b homozygosis were detected which were found in other racial groups. One homozygosis of HPA-6b/6b in 200 individuals was detected which was not found in a study involving 1000 Chinese (Feng et al., 2006).
  
  
  CONCLUSIONS
  The HPA-3 and -15 appear to the highest priority and HPA-2, -6, -5 -1 and -4 to be the second priority in Chinese Cantonese when it comes to the diagnosis of neonatal alloimmune thrombocytopenia and to provide the HPA-matched platelet for patients with platelet transfusion refractoriness. The PCR-SSP method makes it possible to detect genotype of HPA-1 to -16 in less than 4 h and to establish a donor database for HPA genotype in a blood bank.
authors.affiliation: Institute of Clinical Blood Transfusion, Guangzhou Blood Center Division of Gastrointestinal & Pancreatic Surgery, The First Affiliated Hospital of Sun Yet-sen University, Guangzhou, China.
authors.forename: Y-M
authors.initials: YM
authors.lastname: Nie
authors.forename: H-J
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Y-S
authors.initials: YS
authors.lastname: Fu
authors.forename: C-X
authors.initials: CX
authors.lastname: Wang
authors.forename: J-P
authors.initials: JP
authors.lastname: Ma
mesh_terms: D000328:Adult; D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001782:Blood Donors; D002681:China; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D055815:Young Adult
chemical_list: D016824:Antigens, Human Platelet; D017931:DNA Primers