title: [Molecular characteristics of the full-length genome of two new Japanese encephalitis virus isolated from mosquitoes in Hubei province].
abstract: OBJECTIVE
  To sequence and analyze the complete genome of two new Japanese encephalitis virus (JEV) strains isolated from mosquitoes collected in Hubei province in 2008, and to understand the molecular biological characteristics of JEV in this area.
  
  
  METHODS
  RT-PCR was used to amplify the fragments of HBZG08-09 strain and HBZG08-55 strain with 16 pairs overlapping primers after they had been recovered and identified, then the full-length genome was obtained by sequencing and splicing. Biological sequence alignment, nucleotide and amino acid sequence analysis, phylogenetic analysis and analysis of amino acid differences were performed by the software of Clustal X (1.83), MegAlign, Mega (4.0) and Genedoc (3.2).
  
  
  RESULTS
  The genome of two new strains were both 10 965 nucleotides in length with a single open reading frame from 96 to 10 392 coding for a 3432 amino acid poly-protein, the homology of nucleotide and amino acid sequence between two isolates were 98.2% and 99.7% respectively. Further study showed that the new strains were both belonging to genotype I. Two new strains were most closely related to isolates obtained from Henan and Zhejiang province in recent years. Compared with the live attenuated vaccine strain SA-14-14-2 in China, HBZG08-09 strain had 82 amino acid divergence; HBZG08-55 had 84 amino acid divergences. But the amino acid difference occurred in sites were not the key ones affecting the toxicity or antigenic of JEV.
  
  
  CONCLUSIONS
  Two new JEV isolates were both belonging to genotype I, and the key sites of amino acid were not changed.
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Hong-Yue
authors.initials: HY
authors.lastname: Jiang
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Yang
authors.forename: Pei-Long
authors.initials: PL
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Feng-Xia
authors.initials: FX
authors.lastname: Meng
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Guo
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Mei
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: He
authors.forename: Huan-Yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Zi-Jun
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Yang
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D002681:China; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral