Association of p53 codon 72 genetic polymorphism with the risk of ulcerative colitis in northern Iran.
OBJECTIVE
Ulcerative colitis (UC) is a chronic inflammatory condition of the large bowel of unknown etiology, characterized by the presence of bloody diarrhea and mucus associated with a negative stool culture for bacteria, ova, or parasites. The aim of this study was to investigate the association of p53 codon 72 genetic polymorphism with the risk of UC in northern Iran.


METHODS
We evaluated the association of the p53 codon 72 genetic polymorphism with UC in northern Iran. The genotype of 190 patients with UC (115 men, 75 women; mean age, 32 ± 8.6 years) and 220 healthy control subjects (123 men, 97 women; mean age, 33 ± 2.5 years) were compared. Genomic DNA was extracted from colonic bioptic tissues of patients and blood samples of healthy individuals. Genotypes and allele frequencies were determined in patients and controls using allele-specific PCR (AS-PCR).


RESULTS
There were significant differences in the distribution of the polymorphism between the control subjects and the UC patients (P < 0.0001). Significantly increased frequencies of the Pro allele and the Pro/Pro genotype were observed in patients with UC compared with controls (Pro allele: P < 0.0001; odds ratio, 7.87; 95% confidence interval, 4.03-15.35; Pro/Pro: P < 0.0001; odds ratio, 35.21; 95% confidence interval, 12.56-98.73).


CONCLUSIONS
The p53 codon 72 genetic polymorphism is associated with UC in northern Iran.
Vaji
Salaheddin
S
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Salehi
Zivar
Z
Aminian
Keyvan
K
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
D003062:Codon; D016159:Tumor Suppressor Protein p53