[Expression of MDR1 and KIT in imatinib-resistant gastrointestinal stromal tumor cells].
OBJECTIVE
To explore the relationship between imatinib resistance and genes MDR1 and KIT in gastrointestinal stromal tumor (GIST) cells.


METHODS
The MDR1 and KIT mRNA level in GIST882-R and GIST882-S cells were detected by RT-PCR. Immunocytochemistry and Western blot were employed to detect P-gp and CD117 expression in GIST882-R and GIST882-S cells.


RESULTS
The relative expression of MDR1 mRNA was 0.321 + or - 0.033 in GIST882-R and 0.157 + or - 0.056 in GIST882-S cells, and the difference was statistically significant (P<0.05). The relative expression of KIT mRNA was 0.389 + or - 0.063 in GIST882-R and 0.339 + or - 0.067 in GIST882-S, and the difference was not statistically significant (P>0.05). The relative density of P-gp was 0.443 + or - 0.058 in GIST882-R and 0.237 + or - 0.094 in GIST882-S, and the difference was statistically significant (P<0.05). The relative density of CD117 was 0.744 + or - 0.123 in GIST882-R and 0.704 + or - 0.094 in GIST882-S, and the difference was not statistically significant (P>0.05).


CONCLUSIONS
Over-expression of gene MDR1 may be associated with imatinib resistance in GIST. KIT may not be involved in imatinib resistance.
Liu
Jing-lei
JL
Department of General Surgery, Zhongshan Hospital, Fudan Universtity, Shanghai 200032, China.
Qin
Jing
J
Yao
Li-qing
LQ
Hou
Ying-yong
YY
Sun
Yi-hong
YH
Shen
Kun-tang
KT
Su
Ping
P
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D001549:Benzamides; D015815:Cell Adhesion Molecules / Q000235:genetics; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics*; D006801:Humans; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D011743:Pyrimidines / Q000494:pharmacology*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001549:Benzamides; D015815:Cell Adhesion Molecules; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; C054291:antigen 117, Dictyostelium; D000068877:Imatinib Mesylate; D019009:Proto-Oncogene Proteins c-kit