title: Screening of pectinase-producing microorganisms with polygalacturonase activity.
abstract: The aim of this work was to perform the screening of microorganisms, previously isolated from samples of agro-industrial waste and belonging to the culture collection of our laboratory, able to produce polygalacturonases (PG). A total of 107 microorganisms, 92 newly isolated and 15 pre-identified, were selected as potential producers of enzymes with PG activity. From these microorganisms, 20 strains were able to synthesize PG with activities above 3 U mL(-1). After the kinetic study, the enzyme activity was increased up to 13 times and the microorganism identified as Aspergillus niger ATCC 9642 and the newly isolated W23, W43, and D2 (Penicillium sp.) after 24 h of fermentation led to PG activities of 30, 41, 43, and 45 U mL(-1), respectively. The RAPD analysis demonstrated that the selected strains differs genetically, indicating that no duplication of strains among them in the experiments for polygalacturonases production was verified.
authors.affiliation: Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim, Rio Grande do Sul, Brazil.
authors.forename: Jamile
authors.initials: J
authors.lastname: Zeni
authors.forename: Karine
authors.initials: K
authors.lastname: Cence
authors.forename: Camila Elis
authors.initials: CE
authors.lastname: Grando
authors.forename: Lídia
authors.initials: L
authors.lastname: Tiggermann
authors.forename: Rosicler
authors.initials: R
authors.lastname: Colet
authors.forename: Lindomar A
authors.initials: LA
authors.lastname: Lerin
authors.forename: Rogério L
authors.initials: RL
authors.lastname: Cansian
authors.forename: Geciane
authors.initials: G
authors.lastname: Toniazzo
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: Eunice
authors.initials: E
authors.lastname: Valduga
mesh_terms: D005285:Fermentation; D005658:Fungi / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011096:Polygalacturonase