title: ADAMTS-1: a new human ovulatory gene and a cumulus marker for fertilization capacity.
abstract: ADAM-metallopeptidase with thrombospondin type 1 motifs-1 (ADAMTS-1) null female mice show impaired follicular development and ovulatory processes. However, ADAMTS-1 expression and function in human normal ovulation and folliculogenesis have not yet been determined. The objective of this study is to study the expression patterns of ADAMTS-1 in human granulosa cells (GCs) obtained from follicles aspirated during in vitro maturation (IVM) and in vitro fertilization (IVF) procedures. We found that ADAMTS-1 expression is a luteinizing hormone/human chorionic gonadotropin (LH/hCG)-induced gene whose expression in the mural GCs directly correlated with antral follicular growth. Interestingly, we were able to show a significant correlation between ADAMTS-1 expression in cumulus cells and the fertilization capacity of the related oocyte. In conclusion, human ADAMTS-1 is an ovulatory gene and its expression is LH/hCG- and follicle-size dependent. The correlation between its expression in cumulus GCs and oocyte fertilization capacity suggests a role for ADAMTS-1 in human cumulus function.
authors.affiliation: IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. yuval.yung@sheba.health.gov.il
authors.forename: Yuval
authors.initials: Y
authors.lastname: Yung
authors.forename: Ettie
authors.initials: E
authors.lastname: Maman
authors.forename: Sarah
authors.initials: S
authors.lastname: Konopnicki
authors.forename: Berta
authors.initials: B
authors.lastname: Cohen
authors.forename: Masha
authors.initials: M
authors.lastname: Brengauz
authors.forename: Irina
authors.initials: I
authors.lastname: Lojkin
authors.forename: Mariabeatrice
authors.initials: M
authors.lastname: Dal Canto
authors.forename: Rubens
authors.initials: R
authors.lastname: Fadini
authors.forename: Jehoshua
authors.initials: J
authors.lastname: Dor
authors.forename: Ariel
authors.initials: A
authors.lastname: Hourvitz
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000071097:ADAMTS1 Protein; D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D048429:Cell Size / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology / Q000627:therapeutic use; D054885:Cumulus Cells / Q000378:metabolism*; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology / Q000627:therapeutic use; D005306:Fertilization / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development; D010060:Ovulation / Q000235:genetics*; D010062:Ovulation Induction / Q000379:methods; D011379:Prognosis
chemical_list: D015415:Biomarkers; D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human