Comprehensive analysis of DNA-methylation in mammalian tissues using MeDIP-chip.
Genome-wide mapping of epigenetic changes is essential for understanding the mechanisms involved in gene regulation during cell differentiation and embryonic development. DNA-methylation is one of these key epigenetic marks that is directly linked to gene expression is. Methylated DNA immunoprecipitation (MeDIP) is a recently devised method used to determine the distribution of DNA-methylation within functional regions (e.g., promoters) or in the entire genome robustly and cost-efficiently. This approach is based on the enrichment of methylated DNA with an antibody that specifically binds to 5-methyl-cytosine and can be combined with PCR, microarrays or high-throughput sequencing. This article outlines the experimental procedure of MeDIP-chip and provides a comprehensive summary of quality control strategies and primary data analysis.
Pälmke
Nina
N
Institut für Genetik, FB Biowissenschaften, Universität des Saarlandes, Saarbrücken, Germany.
Santacruz
Diana
D
Walter
Jörn
J
D000818:Animals; D004247:DNA / Q000737:chemistry*; D053938:DNA Fragmentation; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D047468:Immunoprecipitation / Q000379:methods*; D008322:Mammals / Q000235:genetics; D013010:Sonication
D004247:DNA