Single-molecule two-colour coincidence detection to probe biomolecular associations.
Two-colour coincidence detection (TCCD) is a form of single-molecule fluorescence developed to sensitively detect and characterize associated biomolecules without any separation, in solution, on the cell membrane and in live cells. In the present short review, we first explain the principles of the method and then describe the application of TCCD to a range of biomedical problems and how this method may be developed further in the future to try to monitor biological processes in live cells.
Orte
Angel
A
Department of Physical Chemistry, Faculty of Pharmacy, Campus Cartuja, Granada 18071, Spain.
Clarke
Richard
R
Klenerman
David
D
D000818:Animals; D003116:Color; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D046911:Macromolecular Substances