title: Establishment and characterization of an experimental model of coronary thrombotic microembolism in rats.
abstract: To establish a model of coronary thrombotic microembolism in rats, either automicrothrombotic particulates (CM group) or saline control (SHAM group) was injected into temporarily clamped aortas of male Sprague-Dawley rats. After automicrothrombotic particulate injection, serum c-troponin I and von Willebrand factor levels, the no-flow area as evaluated by Thioflavin S, myocardial leukocyte infiltration levels, myocardial expressions of tumor necrosis factor alpha and interleukin-6, the percentage of arterioles obstructed by thrombosis, and myocardial fibrosis were all significantly increased whereas cardiac function as evaluated by echocardiography and hemodynamic measurements were significantly reduced compared with the sham group. Thus, aortic automicrothrombotic particulate injection could induce coronary microembolism in rats, and this model could be of value in improving the understanding of pathophysiology of coronary microembolism.
authors.affiliation: Department of Cardiology, Puai Hospital, Huazhong University of Science and Technology, Wuhan, Hubei Province, China. yegu2003cn@163.com
authors.forename: Ye
authors.initials: Y
authors.lastname: Gu
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Bai
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Liqun
authors.initials: L
authors.lastname: Hu
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003327:Coronary Disease / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013923:Thromboembolism / Q000378:metabolism / Q000473:pathology*; D019210:Troponin I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D019210:Troponin I; D014409:Tumor Necrosis Factor-alpha