Increase of reactive oxygen species by desferrioxamine during experimental Chagas' disease.
Oxidative stress is common in inflammatory processes associated with many diseases including Chagas' disease. The aim of the present study was to evaluate, in a murine model, biomarkers of oxidative stress together with components of the antioxidant system in order to provide an overview of the mechanism of action of the iron chelator desferrioxamine (DFO). The study population comprised 48 male Swiss mice, half of which were treated daily by intraperitoneal injection of DFO over a 35-day period, while half were administered sterile water in a similar manner. On the 14th day of the experiment, 12 DFO-treated mice and an equal number of untreated mice were experimentally infected with Trypanosoma cruzi. Serum concentrations of nitric oxide and superoxide dismutase and hepatic levels of total glutathione, thiobarbituric acid reactive species and protein carbonyl, were determined on days 0, 7, 14 and 21 post-infection. The results obtained revealed that DFO enhances antioxidant activity in the host but also increases oxidative stress, indicating that the mode of action of the drug involves a positive contribution to the host together with an effect that is not beneficial to the parasite.
Francisco
Amanda Fortes
AF
Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.
de Abreu Vieira
Paula Melo
PM
Arantes
Jerusa Marilda
JM
Silva
Maisa
M
Pedrosa
Maria Lúcia
ML
Elói-Santos
Silvana Maria
SM
Martins-Filho
Olindo Assis
OA
Teixeira-Carvalho
Andréa
A
Araújo
Márcio Sobreira Silva
MS
Tafuri
Washington Luiz
WL
Carneiro
Cláudia Martins
CM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D014355:Chagas Disease / Q000188:drug therapy* / Q000378:metabolism* / Q000469:parasitology; D003676:Deferoxamine / Q000494:pharmacology*; D004195:Disease Models, Animal; D007502:Iron Chelating Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017262:Siderophores / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000502:physiology
D000975:Antioxidants; D007502:Iron Chelating Agents; D017382:Reactive Oxygen Species; D017262:Siderophores; D013482:Superoxide Dismutase; D003676:Deferoxamine