Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle.
A novel approach was applied in this study to directly evaluate the effect of muscle fiber type on postmortem protein degradation. Porcine muscle fibers were isolated from longissimus muscle at day 1, 3, and 6 postmortem. Fibers were sorted by immunochemical myosin heavy chain isoform typing. Western blot analysis of fibers pooled separately into type I or IIb showed that the relative amounts of 39- and 50-kDa desmin degradation fragments at day 6, and 28- to 31-kDa fragments of troponin T fast type isoform (fTnT) at day 1 and 6 postmortem were higher in type IIb than in type I fibers. At day 6 troponin T slow type isoform (sTnT) was less degraded than fTnT in type I fibers. These results indicated greater rate and extent of proteolysis in type IIb than in type I fibers and higher susceptibility of fTnT to proteolysis than that of sTnT isoform.
Muroya
Susumu
S
University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. muros@affrc.go.jp
Ertbjerg
Per
P
Pomponio
Luigi
L
Christensen
Mette
M
D000818:Animals; D015153:Blotting, Western; D003893:Desmin / Q000378:metabolism*; D008460:Meat; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D011180:Postmortem Changes; D020033:Protein Isoforms / Q000378:metabolism; D013552:Swine; D020107:Troponin T / Q000378:metabolism*
D003893:Desmin; D020033:Protein Isoforms; D020107:Troponin T; D018995:Myosin Heavy Chains