Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis.
Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPAR/ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPAR/, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.
Huang
Jianfeng
J
Huazhong Agricultural University, Wuhan, P R China.
Zhang
Jian
J
Lei
Ting
T
Chen
Xiaodong
X
Zhang
Yan
Y
Zhou
Lulu
L
Yu
An
A
Chen
Zhilong
Z
Zhou
Ronghua
R
Yang
Zaiqing
Z
D054392:Adipokines / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D050155:Lipogenesis; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000378:metabolism; D019707:Receptors, Chemokine / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013552:Swine; D014018:Tissue Distribution
D054392:Adipokines; D051741:Kruppel-Like Transcription Factors; D047495:PPAR gamma; D019707:Receptors, Chemokine; D043562:Receptors, G-Protein-Coupled