Establishment of an axenic culture of microcystin-producing Microcystis aeruginosa isolated from a Korean reservoir.
In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.
Han
Ah-Won
AW
Department of Environmental Engineering, Chungbuk National University, Cheongju, Republic of Korea.
Oh
Kyoung-Hee
KH
Jheong
Weon-Hwa
WH
Cho
Young-Cheol
YC
D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007723:Korea; D052998:Microcystins / Q000235:genetics / Q000378:metabolism*; D046931:Microcystis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D052998:Microcystins; C078588:microcystin