[Human leukocyte antigen-G 14 bp deletion polymorphism in severe pre-eclampsia].
OBJECTIVE
To investigate the relationship between human leukocyte antigen-G (HLA-G) gene Exon 8 14 bp deletion polymorphism and the pathogenesis of severe pre-eclampsia.


METHODS
Forty-two pregnant women with severe pre-eclampsia, who admitted to the Third Affiliated Hospital of Zhengzhou University from October 2008 to February 2009, and their newborns were chosen as the severe pre-eclampsia group. Another 45 healthy gravidas at the third trimester and their newborns were chosen as the control. All gravidas in both groups were Han Nationality. HLA-G Exon 8 genotyping was detected by PCR in both groups and the allele frequencies and genotype frequencies were compared between the two groups. The genotype frequencies of maternal-neonatal pairs were also analyzed.


RESULTS
(1) In the severe pre-eclampsia group, 14% of the maternal-neonatal pairs were homozygote of 14 bp deletion, and significantly higher frequency 33% (15/45) was found in the control group (P=0.038). (2) No significant difference was found in the allele frequencies and genotype frequencies of HLA-G 14 bp deletion polymorphism among all the mothers between the two groups (P>0.05). (3) The +14 bp and -14 bp allele frequencies of HLA-G 14 bp deletion polymorphism in newborns in the severe pre-eclampsia group were 44% (37/84) and 56% (47/84), respectively, and 30% (27/90) and 70% (63/90) in the control group. Although there was no significant difference between the two groups, but differences in trends was identified (chi2=3.678 P=0.055); The genotype (-14 bp/-14 bp) frequency of neonates in the severe pre-eclampsia group showed no difference compared with that in the control group [29% (12/42) vs 49% (22/45)], but differences in trends was also found (P=0.052).


CONCLUSIONS
HLA-G 14 bp deletion polymorphism is associated with the susceptibility of severe pre-eclampsia in Chinese Han nationality. Maternal-fetal genotype pairs of -14 bp/-14 bp may have reduced risk of severe pre-eclampsia.
Zhang
Zhan
Z
Department of Obstertrics and Gynecology, Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. zhangzhanmd@ yahoo.com.cn
Wang
Jing-yan
JY
Zhang
Lin-lin
LL
Guo
Shang
S
Jia
Li-ting
LT
Li
Hui
H
Li
Jing
J
Ju
Juan
J
Rong
Shou-hua
SH
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002681:China / Q000208:ethnology; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D011247:Pregnancy; D017384:Sequence Deletion; D012720:Severity of Illness Index
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I