Interfacing living unicellular algae cells with biocompatible polyelectrolyte-stabilised magnetic nanoparticles.
Green algae are a promising platform for the development of biosensors and bioelectronic devices. Here we report a reliable single-step technique for the functionalisation of living unicellular green algae Chlorella pyrenoidosa with biocompatible 15 nm superparamagnetic nanoparticles stabilised with poly(allylamine hydrochloride). The magnetised algae cells can be manipulated and immobilised using external permanent magnets. The distribution of the nanoparticles on the cell walls of C. pyrenoidosa was studied by optical and fluorescence microscopy, TEM, SEM and EDX spectroscopy. The viability and the magnetic properties of the magnetised algae are studied in comparison with the native cells. The technique may find a number of potential applications in biotechnology and bioelectronics.
Fakhrullin
Rawil F
RF
Biomaterials and Nanomaterials Group, Department of Biochemistry, Kazan (Idel-Ural) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan.
Shlykova
Lubov V
LV
Zamaleeva
Alsu I
AI
Nurgaliev
Danis K
DK
Osin
Yuri N
YN
García-Alonso
Javier
J
Paunov
Vesselin N
VN
D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D002708:Chlorella / Q000166:cytology*; D008280:Magnetics; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D001672:Biocompatible Materials; D011108:Polymers