title: Silencing of cytosolic NADP+-dependent isocitrate dehydrogenase gene enhances ethanol-induced toxicity in HepG2 cells.
abstract: It has been shown that acute and chronic alcohol administrations increase the production of reactive oxygen species, lower cellular antioxidant levels and enhance oxidative stress in many tissues. We recently reported that cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDPc) functions as an antioxidant enzyme by supplying NADPH to the cytosol. Upon exposure to ethanol, IDPc was susceptible to the loss of its enzyme activity in HepG2 cells. Transfection of HepG2 cells with an IDPc small interfering RNA noticeably downregulated IDPc and enhanced the cells' vulnerability to ethanol-induced cytotoxicity. Our results suggest that suppressing the expression of IDPc enhances ethanol-induced toxicity in HepG2 cells by further disruption of the cellular redox status.
authors.affiliation: Kyungpook National University, Taegu, Korea.
authors.forename: Eun Sun
authors.initials: ES
authors.lastname: Yang
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Jeen-Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D003600:Cytosol / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology; D000431:Ethanol / Q000633:toxicity*; D020868:Gene Silencing / Q000187:drug effects* / Q000502:physiology*; D056945:Hep G2 Cells; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000502:physiology; D009249:NADP / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D000431:Ethanol; D009249:NADP; D007521:Isocitrate Dehydrogenase