title: Anti-obesity effect of Morus bombycis root extract: anti-lipase activity and lipolytic effect.
abstract: OBJECTIVE
  This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues.
  
  
  METHODS
  Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured.
  
  
  RESULTS
  Morus bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07 microg/mL.In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner.
  
  
  CONCLUSIONS
  The present study suggests that Morus bombycis root extract might be of therapeutic interest with respect to the treatment of obesity.
authors.affiliation: Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Hyojun
authors.initials: H
authors.lastname: Kim
authors.forename: Junghyun
authors.initials: J
authors.lastname: Kim
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.forename: Joo Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005990:Glycerol / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D031625:Morus / Q000737:chemistry*; D009765:Obesity / Q000188:drug therapy; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019440:Anti-Obesity Agents; D010726:Phosphodiesterase Inhibitors; D010936:Plant Extracts; D014280:Triglycerides; D010727:Phosphoric Diester Hydrolases; D005990:Glycerol