title: Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells.
abstract: Mesenchymal stromal/stem cells (MSC), when used in combination with biomaterial scaffolds, have been shown to contribute at varying efficiencies to bone and cartilage regeneration in preclinical large animal models and human clinical trials. In an orthopedic context, identification of the optimal scaffold, which is capable of inducing tissue regeneration, has been the subject of numerous studies. In the present study, we show that ex vivo-expanded MSC from human and ovine bone marrow display similar phenotypic properties, but exhibit differences in their ability to form bone in vivo when transplanted with different biocompatible scaffold composites. We found that the ovine MSC formed ectopic bone on all scaffolds tested with the exception of collagen-based demineralized bone matrix. In contrast, human MSC in general formed less bone and only on those biomaterials composed of ceramic particles containing at least 15% hydroxyapatite. This study demonstrates the differences in bone formation potential between human and ovine MSC in vivo based on the osteoconductive properties of different bioscaffolds currently being used for orthopedic clinical applications.
authors.affiliation: Myeloma Research Laboratory, Department of Haematology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
authors.forename: Andrew C W
authors.initials: AC
authors.lastname: Zannettino
authors.forename: Sharon
authors.initials: S
authors.lastname: Paton
authors.forename: Silviu
authors.initials: S
authors.lastname: Itescu
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
mesh_terms: D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D016513:Mice, SCID; D010012:Osteogenesis / Q000502:physiology; D012756:Sheep; D017154:Stromal Cells / Q000166:cytology* / Q000502:physiology; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite