title: Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation.
abstract: In eukaryotic cells, autophagy is a highly conserved self-digestion process to promote cell survival in response to nutrient starvation and other metabolic stresses. Autophagy is regulated by cell signalling such as the mTOR (mammalian target of rapamycin) pathway. However, the significance of autophagy in modulation of signal transduction is unclear. Here we show that autophagy negatively regulates Wnt signalling by promoting Dishevelled (Dvl) degradation. Von Hippel-Lindau protein-mediated ubiquitylation is critical for the binding of Dvl2 to p62, which in turn facilitates the aggregation and the LC3-mediated autophagosome recruitment of Dvl2 under starvation; the ubiquitylated Dvl2 aggregates are ultimately degraded through the autophagy-lysosome pathway. Moreover, a reverse correlation between Dvl expression and autophagy is observed in late stages of colon cancer development, indicating that autophagy may contribute to the aberrant activation of Wnt signalling in tumour formation.
authors.affiliation: The State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Gao
authors.forename: Weipeng
authors.initials: W
authors.lastname: Cao
authors.forename: Lan
authors.initials: L
authors.lastname: Bao
authors.forename: Wei
authors.initials: W
authors.lastname: Zuo
authors.forename: Guoming
authors.initials: G
authors.lastname: Xie
authors.forename: Tiantian
authors.initials: T
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Fu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Ye-Guang
authors.initials: YG
authors.lastname: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology; D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D000071186:Beclin-1; D002460:Cell Line; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism; D000072261:Dishevelled Proteins; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D016253:Microscopy, Immunoelectron; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008954:Models, Biological; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071456:Sequestosome-1 Protein; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D054875:Ubiquitination; D051153:Wnt Proteins / Q000378:metabolism*; D060508:Wnt3 Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; C000606581:DVL2 protein, human; D000072261:Dishevelled Proteins; C116741:GABARAP protein, human; C477012:MAP1LC3A protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; D051153:Wnt Proteins; D060508:Wnt3 Protein