title: Effect of a single oral dose of 600,000 IU of cholecalciferol on serum calciotropic hormones in young subjects with vitamin D deficiency: a prospective intervention study.
abstract: BACKGROUND
  Effects of vitamin D repletion in young people with low vitamin D status have not been investigated so far.
  
  
  OBJECTIVE
  We evaluated the effect of a single massive dose of cholecalciferol on calcium metabolism at 3, 15, and 30 d, compared to baseline.
  
  
  METHODS
  We conducted a prospective intervention study in an ambulatory care setting.
  
  
  METHODS
  Forty-eight young subjects with vitamin D deficiency participated in the study.
  
  
  METHODS
  A single oral dose of 600,000 IU of cholecalciferol was administered to each subject.
  
  
  METHODS
  We evaluated serum changes of 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D, calcium, and PTH induced by a single load of cholecalciferol.
  
  
  RESULTS
  The 25(OH)D level was 15.8 ± 6.5 ng/ml at baseline and became 77.2 ± 30.5 ng/ml at 3 d (P < 0.001) and 62.4 ± 26.1 ng/ml at 30 d (P < 0.001). PTH levels concomitantly decreased from 53.0 ± 20.1 to 38.6 ± 17.2 pg/ml at 3 d and to 43.4 ± 14.0 pg/ml at 30 d (P < 0.001 for both). The trends were maintained in a subgroup followed up to 90 d (P < 0.001). Mean serum Ca and P significantly increased compared to baseline, whereas serum Mg decreased at 3 d. 1,25-Dihydroxyvitamin D significantly increased from 46.8 ± 18.9 to 97.8 ± 38.3 pg/ml at 3 d (P < 0.001) and to 59.5 ± 27.3 pg/ml at 60 d (P < 0.05).
  
  
  CONCLUSIONS
  A single oral dose of 600,000 IU of cholecalciferol rapidly enhances 25(OH)D and reduces PTH in young people with vitamin D deficiency.
authors.affiliation: Department of Clinical Sciences, University of Rome Sapienza, Rome, Italy. cristianac@alice.it
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cipriani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Romagnoli
authors.forename: Alfredo
authors.initials: A
authors.lastname: Scillitani
authors.forename: Iacopo
authors.initials: I
authors.lastname: Chiodini
authors.forename: Rita
authors.initials: R
authors.lastname: Clerico
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Carnevale
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Mascia
authors.forename: Claudia
authors.initials: C
authors.lastname: Battista
authors.forename: Raffaella
authors.initials: R
authors.lastname: Viti
authors.forename: Mauro
authors.initials: M
authors.lastname: Pileri
authors.forename: Cristina
authors.initials: C
authors.lastname: Eller-Vainicher
authors.forename: Salvatore
authors.initials: S
authors.lastname: Minisola
mesh_terms: D000284:Administration, Oral; D000328:Adult; D002118:Calcium / Q000097:blood; D002762:Cholecalciferol / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006728:Hormones / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010281:Parathyroid Hormone / Q000097:blood; D011446:Prospective Studies; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood* / Q000188:drug therapy*; D055815:Young Adult
chemical_list: D006728:Hormones; D010281:Parathyroid Hormone; D014807:Vitamin D; D002762:Cholecalciferol; C097949:1,25-dihydroxyvitamin D; C104450:25-hydroxyvitamin D; D002118:Calcium