Subcellular controls of mercury trophic transfer to a marine fish.
Different behaviors of inorganic mercury [Hg(II)] and methylmercury (MeHg) during trophic transfer along the marine food chain have been widely reported, but the mechanisms are not fully understood. The bioavailability of ingested mercury, quantified by assimilation efficiency (AE), was investigated in a marine fish, the grunt Terapon jarbua, based on mercury subcellular partitioning in prey and purified subcellular fractions of prey tissues. The subcellular distribution of Hg(II) differed substantially among prey types, with cellular debris being a major (49-57% in bivalves) or secondary (14-19% in other prey) binding pool. However, MeHg distribution varied little among prey types, with most MeHg (43-79%) in heat-stable protein (HSP) fraction. The greater AEs measured for MeHg (90-94%) than for Hg(II) (23-43%) confirmed the findings of previous studies. Bioavailability of each purified subcellular fraction rather than the proposed trophically available metal (TAM) fraction could better elucidate mercury assimilation difference. Hg(II) associated with insoluble fraction (e.g. cellular debris) was less bioavailable than that in soluble fraction (e.g. HSP). However, subcellular distribution was shown to be less important for MeHg, with each fraction having comparable MeHg bioavailability. Subcellular distribution in prey should be an important consideration in mercury trophic transfer studies.
Dang
Fei
F
Department of Biology, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong.
Wang
Wen-Xiong
WX
D000704:Analysis of Variance; D000818:Animals; D001682:Biological Availability; D004785:Environmental Pollutants / Q000493:pharmacokinetics*; D020387:Food Chain; D006723:Hong Kong; D008628:Mercury / Q000493:pharmacokinetics*; D008631:Mercury Radioisotopes / Q000032:analysis; D008767:Methylmercury Compounds / Q000493:pharmacokinetics*; D010473:Perciformes / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D004785:Environmental Pollutants; D008631:Mercury Radioisotopes; D008767:Methylmercury Compounds; D008628:Mercury