Subcellular localisation of the p40phox component of NADPH oxidase involves direct interactions between the Phox homology domain and F-actin.
Cytosolic components of the NADPH oxidase interact with the actin cytoskeleton. These interactions are thought to be important for the activation of this enzyme system but they are poorly characterised at the molecular level. Here we have explored the interaction between the actin cytoskeleton and p40(phox), one of the cytosolic components of NADPH oxidase. Full length p40(phox) expressed in COS cells co-localised with F-actin in a peripheral lamellar compartment. The co-localisation was lost after deletion of the Phox homology (PX) domain and the PX domain in isolation (p40PX) showed the same F-actin co-localisation as the full length protein. PX domains are known lipid-binding modules however, a mutant p40PX which did not bind lipids still co-localised with F-actin suggesting that lipid-independent interactions underlie the localisation. Affinity chromatography identified actin as a binding partner for p40PX in neutrophil extracts. Pure actin interacted with both p40(phox) and with p40PX suggesting it is a direct interaction. Disruption of the actin cytoskeleton with cytochalasin D resulted in actin rearrangement and concomitantly the localisation of full length p40(phox) proteins and that of p40PX changed. Thus p40PX is a dual F-actin/lipid-binding module and F-actin interactions with the PX domain dictate at least in part the intracellular localisation of the cytosolic p40(phox) subunit of the NADPH oxidase.
Shao
Dongmin
D
Department of Medicine, University College London, London, UK.
Segal
Anthony W
AW
Dekker
Lodewijk V
LV
D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D002457:Cell Extracts; D002522:Chlorocebus aethiops; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics
D000199:Actins; D002457:Cell Extracts; D010750:Phosphoproteins; C085072:neutrophil cytosol factor 40K; D015638:Cytochalasin D; D019255:NADPH Oxidases