title: [The expression of sonic hedgehog in rat vein grafts].
abstract: OBJECTIVE
  To study the expression of cell cycle related factor sonic hedgehog (SHH) in autogenous vein graft and its relation with neointima formation.
  
  
  METHODS
  Autogenous vein graft model were established in 24 male Wistar rats of 8 weeks old and 140 g weight, by transplanting the left jugular vein to intra renal abdominal aorta with microsurgical technique. Graft veins were harvested at 14 d and 28 d after transplantation. The immunohistochemistry and Western blot were used to detect the SHH and PCNA expression in the vein graft. At the same time SHH mRNA was measured by quantitative real-time PCR. The opposite normal veins served as control.
  
  
  RESULTS
  Histological staining showed that the percent of SHH+ cells was only (2.0 +/- 0.5)% in the normal vein, but was much more in the vein graft after surgery, as (39.4 +/- 0.4)% and (63.0 +/- 0.3)% respectively (P < 0.01). The expression of SHH and PCNA were both elevated in the vein graft. There was a positive correlation between them which indicated by Western blot (r = 0.808, P < 0.01). The SHH mRNA content also increased in vein graft to 9.5 and 23.8 folds of that in control.
  
  
  CONCLUSIONS
  SHH is upregulated in autogenous vein grafts and may correlated with the proliferation of vascular smooth muscle cells.
authors.affiliation: Department of Vascular Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors.forename: Feng-he
authors.initials: FH
authors.lastname: Li
authors.forename: Shi-jie
authors.initials: SJ
authors.lastname: Xin
authors.forename: Lin-ping
authors.initials: LP
authors.lastname: Hui
authors.forename: Ze-shi
authors.initials: ZS
authors.lastname: Cui
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-quan
authors.initials: ZQ
authors.lastname: Duan
mesh_terms: D000818:Animals; D053823:Hedgehog Proteins / Q000378:metabolism*; D008297:Male; D058426:Neointima / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000378:metabolism; D014680:Veins / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation
chemical_list: D053823:Hedgehog Proteins