Oxidative stress, Helicobacter pylori, and OGG1 Ser326Cys, XPC Lys939Gln, and XPD Lys751Gln polymorphisms in a Turkish population with colorectal carcinoma.
The contribution of polymorphisms of DNA repair genes OGG1 Ser326Cys, XPC Lys939Gln, and XPD Lys751Gln in developing colorectal carcinoma is controversial. Whether the group 1A carcinogen Helicobacter pylori is a risk factor or not in these patients could not be clearly elucidated. One hundred ten colorectal cancer patients and 116 cancer-free individuals constituted the test and control groups, respectively. The association of OGG1 Ser326Cys, XPC Lys939Gln, and XPD Lys751Gln polymorphisms and the susceptibility to colorectal carcinoma with or without oxidative stress were evaluated. DNA was extracted from peripheral blood cells and genotypes were determined using polymerase chain reaction-restriction fragment length polymorphism. For serum nitric oxide and total antioxidant status assay, spectrophotometric analyses were used. Serum albumin measurements were performed using an autoanalyzer. H. pylori IgG was measured by ELISA. The serum albumin concentrations of cancer patients were significantly lower than those of the controls (p < 0.05). The carriers of the variant genotype of OGG1 (odds ratio: 0.963; 95% confidence interval: 0.446-2.079), XPC (0.789, 0.366-1.700), or XPD (0.532, 0.259-1.094) did not associate with the increased risk of cancer progression, despite the increased oxidative stress in cancer patients. Seropositivity of H. pylori IgG has been found to increase the risk of colorectal carcinoma by 2.2-fold.
Engin
Ayse Basak
AB
Department of Toxicology, Faculty of Pharmacy, Gazi University, Ankara, Turkey. abengin@gmail.com
Karahalil
Bensu
B
Engin
Atilla
A
Karakaya
Ali Esat
AE
D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution / Q000235:genetics / Q000502:physiology; D015179:Colorectal Neoplasms / Q000150:complications / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003545:Cysteine / Q000235:genetics; D045647:DNA Glycosylases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005828:Genetics, Population; D005973:Glutamine / Q000235:genetics; D016481:Helicobacter Infections / Q000097:blood / Q000150:complications / Q000235:genetics / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008239:Lysine / Q000235:genetics; D008875:Middle Aged; D020125:Mutation, Missense / Q000502:physiology; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D012694:Serine / Q000235:genetics; D014421:Turkey; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
D004268:DNA-Binding Proteins; D005973:Glutamine; C086984:XPC protein, human; D012694:Serine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human; D008239:Lysine; D003545:Cysteine