title: Kinetic resolution of (R/S)-propranolol (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) catalyzed by immobilized preparations of Candida antarctica lipase B (CAL-B).
abstract: Enzyme preparations of Candida antarctica B lipase (CAL-B) - immobilized on Eupergit C and partially modified Eupergit C supports - were tested for kinetic resolution of (R/S)-propranolol, using vinyl acetate as acyl donor, and toluene as organic solvent. The effects of (R/S)-propranolol concentration, vinyl acetate concentration and biocatalyst loading on the esterification and resolution of propranolol were studied. Additionally, different types of immobilized lipase derivatives were also evaluated in terms of its selectivity on kinetic resolution of (R,S)-propranolol. These derivatives showed different enantiomeric ratios (E), with high enantiomeric ratios (E=57) with CAL-B immobilized on Eupergit C supports.
authors.affiliation: Escuela de Química, Grupo de investigación en Bioquímica e Ingeniería de proteínas (GIBIP), Edificio Camilo Torres 210, Universidad Industrial de Santander, Bucaramanga, Colombia.
authors.forename: Oveimar
authors.initials: O
authors.lastname: Barbosa
authors.forename: Cesar
authors.initials: C
authors.lastname: Ariza
authors.forename: Claudia
authors.initials: C
authors.lastname: Ortiz
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Torres
mesh_terms: D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000378:metabolism*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry; D011433:Propranolol / Q000031:analogs & derivatives* / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D014050:Toluene / Q000737:chemistry / Q000378:metabolism; D014753:Vinyl Compounds / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011108:Polymers; D012997:Solvents; D014753:Vinyl Compounds; D014050:Toluene; C065843:Eupergit C; D011433:Propranolol; D008049:Lipase; C431349:lipase B, Candida antarctica; C011566:vinyl acetate