title: Associations between matrix metalloproteinase-8 and -14 and myeloperoxidase in gingival crevicular fluid from subjects with progressive chronic periodontitis: a longitudinal study.
abstract: BACKGROUND
  Matrix metalloproteinase (MMP)-8 is a central mediator in chronic periodontitis. MMP-8 can be activated by the cooperative action of other MMPs such as MMP-14, reactive oxygen species, and microbial proteases. The aim of this study is to associate the levels, molecular forms, isoenzyme distribution, and degree of activation of MMP-8 and -14, myeloperoxidase (MPO), and tissue inhibitor of MMP (TIMP)-1 in gingival crevicular fluid (GCF) from patients with progressive periodontitis at baseline and after periodontal therapy.
  
  
  METHODS
  In this longitudinal study, GCF samples from active (n = 25) and inactive (n = 25) sites of subjects with periodontitis were screened at baseline for GCF levels of MMP-8 by immunofluorometric assay, of MMP-14 by specific activity assay, and of MPO and TIMP-1 by enzyme-linked immunosorbent assay. MMP-8 and MPO were also measured after periodontal treatment. Molecular forms were determined by immuno-Western blot analyses and subjected to densitometric scanning and statistical analyses.
  
  
  RESULTS
  High MMP-8 and MPO levels and a strong MPO/MMP-8 positive correlation were found in active and inactive sites at baseline. After treatment, decreases in MPO and MMP-8 were seen, except for active sites in which MMP-8 differences were not significant (P <0.05).
  
  
  CONCLUSIONS
  We present initial data that show that GCF levels and associations between MPO and MMP-8 are related to progression episodes and treatment responses in patients with chronic periodontitis. Our results suggest an interaction between the MPO oxidative pathway and MMP-8 activation, and this cascade might be useful as a potential biomarker for treatment outcomes.
authors.affiliation: Laboratory of Periodontal Biology, Faculty of Dentistry, University of Chile, Santiago, Chile. mhernandezrios@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: J
authors.initials: J
authors.lastname: Gamonal
authors.forename: T
authors.initials: T
authors.lastname: Tervahartiala
authors.forename: P
authors.initials: P
authors.lastname: Mäntylä
authors.forename: O
authors.initials: O
authors.lastname: Rivera
authors.forename: A
authors.initials: A
authors.lastname: Dezerega
authors.forename: N
authors.initials: N
authors.lastname: Dutzan
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000201:enzymology; D055113:Chronic Periodontitis / Q000201:enzymology* / Q000628:therapy; D012534:Dental Scaling; D018450:Disease Progression; D004789:Enzyme Activation; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D008137:Longitudinal Studies; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000032:analysis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D008648:Mesoderm / Q000201:enzymology; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D009910:Oral Hygiene; D017622:Periodontal Attachment Loss / Q000201:enzymology; D010514:Periodontal Pocket / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis*; D016745:Root Planing; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis
chemical_list: D007527:Isoenzymes; D019715:Tissue Inhibitor of Metalloproteinase-1; D009195:Peroxidase; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14