Bile salt-reinforced alginate-chitosan beads.
A polymeric delayed release protein delivery system was investigated with albumin as the model drug. The polysaccharide chitosan was reacted with sodium alginate in the presence of calcium chloride to form beads with a polyelectrolyte. In this study, attempts were made to extend albumin release in the phosphate buffer at pH 6.8 from the alginate-chitosan beads by reinforcing the matrix with bile salts. Sodium taurocholate was able to prevent albumin release at pH 1.2, protecting the protein from the acidic environment and extending the total albumin release at pH 6.8. This effect was explained by an interaction between the permanent negatively charged sulfonic acid of sodium taurocholate with the amino groups of chitosan. Mild formulation conditions, high bovine serum albumin (BSA) entrapment efficiency, and resistance to gastrointestinal release seem to be synergic and promising factors toward the development of an oral protein delivery form.
Takka
Sevgi
S
Pharmaceutical Technology Department, Faculty of Pharmacy, University of Gazi, Etiler, Ankara. sevgitakka@yahoo.com
Cali
Aybige Gürel
AG
D000464:Alginates / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents; D003840:Deoxycholic Acid; D004339:Drug Compounding; D016503:Drug Delivery Systems; D041981:Gastrointestinal Tract / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry; D012995:Solubility; D013656:Taurocholic Acid
D000464:Alginates; D001647:Bile Acids and Salts; D003432:Cross-Linking Reagents; D003840:Deoxycholic Acid; D012710:Serum Albumin, Bovine; D013656:Taurocholic Acid; D048271:Chitosan