title: Handheld impedance biosensor system using engineered proteinaceous receptors.
abstract: We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.
authors.affiliation: Department of Electrical and Computer Engineering, McGill University, Montreal, Canada. ebrahim.ghafar-zadeh@polymtl.ca
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Ghafar-Zadeh
authors.forename: Shafinaz F
authors.initials: SF
authors.lastname: Chowdhury
authors.forename: Amir
authors.initials: A
authors.lastname: Aliakbar
authors.forename: Vamsy
authors.initials: V
authors.lastname: Chodavarapu
authors.forename: Rose
authors.initials: R
authors.lastname: Lumbroso
authors.forename: Rosemond
authors.initials: R
authors.lastname: Lambrose
authors.forename: Lenore K
authors.initials: LK
authors.lastname: Beitel
authors.forename: Lenord
authors.initials: L
authors.lastname: Beital
authors.forename: Mohamad
authors.initials: M
authors.lastname: Sawan
authors.forename: Mark
authors.initials: M
authors.lastname: Trifiro
mesh_terms: D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004867:Equipment Design; D005941:Glucokinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering; D012984:Software; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D005941:Glucokinase; D005947:Glucose