title: Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells.
abstract: Campylobacter jejuni, an important food-borne bacterial pathogen in industrialized countries and in the developing world, is one of the major causes of bacterial diarrhoea. To identify genes which are important for the invasion of host cells by the pathogen, we screened altogether 660 clones of a transposon-generated mutant library based on the clinical C. jejuni isolate B2. Thereby, we identified a clone with a transposon insertion in gene cj0952c. As in the well-characterized C. jejuni strain NCTC 11168, the corresponding protein together with the gene product of the adjacent gene cj0951c consists of two transmembrane domains, a HAMP domain and a putative MCP domain, which together are thought to act as a chemoreceptor, designated Tlp7. In this report we show that genes cj0952c and cj0951c (i) are important for the host cell invasion of the pathogen, (ii) are not translated as one protein in C. jejuni isolate B2, contradicting the idea of a postulated read-through mechanism, (iii) affect the motility of C. jejuni, (iv) alter the chemotactic behaviour of the pathogen towards formic acid, and (v) are not related to the utilization of formic acid by formate dehydrogenase.
authors.affiliation: University Medical Center Göttingen, Institute for Medical Microbiology, Kreuzbergring 57, 37075 Göttingen, Germany.
authors.forename: A Malik
authors.initials: AM
authors.lastname: Tareen
authors.affiliation: University Medical Center Göttingen, Institute for Medical Microbiology, Kreuzbergring 57, 37075 Göttingen, Germany.
authors.forename: Javid Iqbal
authors.initials: JI
authors.lastname: Dasti
authors.affiliation: University Medical Center Göttingen, Institute for Medical Microbiology, Kreuzbergring 57, 37075 Göttingen, Germany.
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Zautner
authors.affiliation: University Medical Center Göttingen, Institute for Medical Microbiology, Kreuzbergring 57, 37075 Göttingen, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Groß
authors.affiliation: University Medical Center Göttingen, Institute for Medical Microbiology, Kreuzbergring 57, 37075 Göttingen, Germany.
authors.forename: Raimond
authors.initials: R
authors.lastname: Lugert
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005561:Formates / Q000737:chemistry*; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005561:Formates; C030544:formic acid