[Culture and polymerase chain reaction of Helicobacter pylori from rectal and terminal ileal fluid after polyethylene glycol (colyte) ingestion in healthy adults with positive urea breath test].
OBJECTIVE
Helicobacter pylori (H. pylori) transmission route is not yet clearly understood. Isolating H. pylori from stool, saliva, and vomitus is very difficult. However, H. pylori could be cultured from feces in the setting of rapid gastrointestinal tract transit. The aim of this study was to isolate H. pylori by culture and PCR in the rectum and terminal ileum during colonoscopy.


METHODS
Twenty subjects with positive UBT (urea breath test) were included. We performed polymerase chain reaction (PCR) test and culture of H. pylori with the rectal fluid and terminal ileal fluid during colonoscopy.


RESULTS
H. pylori was cultured with rectal fluid from 9 (45.0%) of 20 subjects and with ileal fluid from 11 (55.0%) of 20 subjects. H. pylori was a little more frequently cultured from the terminal ileal fluid than the rectal fluid without statistical significance (p>0.05). PCR test detected flaA (16/20, 80.0% and 17/20, 85.0%), 16S rRNA gene (16/20, 80.0% and 17/20, 85.0%), cagA (10/20, 50.0% and 12/20, 60.0%), and ureC (9/20, 45% and 11/20, 54.5%) from the rectal fluid and the terminal ileal fluid, respectively. The specificity and sensitivity of ureC were 100%.


CONCLUSIONS
H. pylori could be cultured from the rectal fluid and terminal ileal fluid in the setting of rapid gastrointestinal tract transit. These results suggest of fecal-oral transmission of H. pylori.
Kim
Do Hyun
DH
Department of Internal Medicine, Saint Carollo Hosipital, Suncheon, Korea.
Jung
Hong Myong
HM
Hwang
Young Jun
YJ
Ahn
Yong Soo
YS
Mun
Jang Sik
JS
Myoung
Bo Hyun
BH
Park
Hyeuk
H
Jeong
Eun Joo
EJ
Im
Yun Mi
YM
Oh
Hyun Min
HM
Jeong
Hui Yeong
HY
Park
Chul
C
Kim
Hyung Rag
HR
Cho
Eun Hae
EH
Kim
Ho Dong
HD
Jun
Young Do
YD
D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001944:Breath Tests; D004573:Electrolytes / Q000008:administration & dosage; D005243:Feces / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000635:transmission; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007082:Ileum / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000008:administration & dosage; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014508:Urea / Q000032:analysis; D014510:Urease / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004573:Electrolytes; C033608:Golytely; D012336:RNA, Ribosomal, 16S; C080866:cagA protein, Helicobacter pylori; D011092:Polyethylene Glycols; D014508:Urea; D014510:Urease