Research resource: Comprehensive expression atlas of the fibroblast growth factor system in adult mouse.
Although members of the fibroblast growth factor (FGF) family and their receptors have well-established roles in embryogenesis, their contributions to adult physiology remain relatively unexplored. Here, we use real-time quantitative PCR to determine the mRNA expression patterns of all 22 FGFs, the seven principal FGF receptors (FGFRs), and the three members of the Klotho family of coreceptors in 39 different mouse tissues. Unsupervised hierarchical cluster analysis of the mRNA expression data reveals that most FGFs and FGFRs fall into two groups the expression of which is enriched in either the central nervous system or reproductive and gastrointestinal tissues. Interestingly, the FGFs that can act as endocrine hormones, including FGF15/19, FGF21, and FGF23, cluster in a third group that does not include any FGFRs, underscoring their roles in signaling between tissues. We further show that the most recently identified Klotho family member, Lactase-like, is highly and selectively expressed in brown adipose tissue and eye and can function as an additional coreceptor for FGF19. This FGF atlas provides an important resource for guiding future studies to elucidate the physiological functions of FGFs in adult animals.
Fon Tacer
Klementina
K
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Bookout
Angie L
AL
Ding
Xunshan
X
Kurosu
Hiroshi
H
John
George B
GB
Wang
Lei
L
Goetz
Regina
R
Mohammadi
Moosa
M
Kuro-o
Makoto
M
Mangelsdorf
David J
DJ
Kliewer
Steven A
SA
D000818:Animals; D001271:Atlases as Topic; D016000:Cluster Analysis; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice / Q000196:embryology / Q000502:physiology*; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000502:physiology*; D014018:Tissue Distribution
C000717427:FGF23 protein, human; C000717428:Fgf23 protein, mouse; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23