Effects of leptin on the expression of alpha1 (I) collagen gene in human osteoblast-like MG63 cells.
The objective of this study was to examine the effects of leptin on alpha (alpha) 1 (I) collagen gene expression in a human osteoblast-like MG63 cell line. MG63 cells were incubated with different doses of leptin (10(-8), 10(-7), and 10(-6) mol x L(-1)) for 24, 48, and 72 h. alpha1 (I) collagen gene expression in MG63 cells was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR), with 17beta-estradiol (17beta-E2) as the positive control. Expression of the alpha1 (I) collagen gene, regulated by leptin, was dose and time dependent, with maximal expression in the 10(-7) mol x L(-1) group at 72 h of incubation. As a positive control, 17beta-E2 reached its maximal effect in the 10(-7) mol x L(-1) group at 24 h. We conclude that leptin has the ability to up-regulate alpha1 (I) collagen gene expression in MG63 cells, with a more potent effect but a less rapid response than 17beta- E2.
Peng
Mian
M
First Affiliated Hospital of Shoutou University Medical College, Shantou, Guangdong, China. pengmian@tom.com
Chen
Shu
S
Fang
Weiqiang
W
Yu
Xuetao
X
D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004958:Estradiol / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
D024042:Collagen Type I; D020738:Leptin; D004958:Estradiol