title: Applications and case studies of the next-generation sequencing technologies in food, nutrition and agriculture.
abstract: The next-generation sequencing technologies are able to produce millions of short sequence reads in a high-throughput, cost-effective fashion. The emergence of these technologies has not only facilitated genome sequencing but also started to change the landscape of life sciences. Here, I survey their major applications ranging from whole-genome sequencing and resequencing, single nucleotide polymorphism (SNP) and structural variation discovery, to mRNA and noncoding RNA profiling and protein-nucleic acid interaction assay. These case studies in structural, functional and comparative genomics, metagenomics, and epigenomics are providing a more complete picture of the genome structures and functions. In the near future, we will witness broad impacts of these next-generation sequencing technologies for solving the complex biological problems in food, nutrition and agriculture. In this article, recent patents based information is also included.
authors.affiliation: USDA, ARS, ANRI, Bovine Functional Genomics Laboratory, Beltsville Agricultural Research Center (BARC) - East, Beltsville, MD 20705, USA. George.Liu@ars.usda.gov
authors.forename: George E
authors.initials: GE
authors.lastname: Liu
mesh_terms: D000383:Agriculture; D044127:Epigenesis, Genetic; D006801:Humans; D056186:Metagenomics; D054647:Nutrigenomics; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*