Suppression of procollagen {alpha}1 type 1 by long-term low-dose exposure to 2-hydroxyethylmethacrylate in human gingival fibroblasts in vitro.
The purpose of this study was to evaluate the cytotoxicity of low doses and long-term exposure to 2-hydroxyethylmethacrylate (HEMA) on the protein expression of human gingival fibroblasts (HGFs). Human gingival fibroblasts were exposed to different concentrations of HEMA ranging from 0.5 mmol/L to 3 mmol/L for periods of time from 72 hours to 2 weeks. A significant decrease in the expression of procollagen α1 type I protein was observed 72 hours after treatment of cells with 3 mmol/L HEMA. Although low concentrations of the monomer after 2 weeks of exposure to HEMA did not appear to induce any marked changes in the morphology or viability of cells, the expression of procollagen α1 type I protein and its messenger RNA (mRNA) markedly decreased. In conclusion, our data demonstrated that cell viability and morphology assays could be deficient parameters in evaluating the biocompatibility of dental resin materials.
Falconi
Mirella
M
Department of Human Anatomical Sciences, University of Bologna, via Irnerio, 48-40126 Bologna, Italy. mirella.falconi@unibo.it
Ortolani
Michela
M
Teti
Gabriella
G
Zago
Michela
M
Orsini
Giovanna
G
Selan
Laura
L
Mazzotti
Giovanni
G
D001672:Biocompatible Materials / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D000090602:Collagen Type I, alpha 1 Chain; D015536:Down-Regulation / Q000187:drug effects*; D005881:Gingiva / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*; D008856:Microscopy, Fluorescence; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D012117:Resins, Synthetic / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D018675:Toxicity Tests / Q000379:methods
D001672:Biocompatible Materials; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D008689:Methacrylates; D012333:RNA, Messenger; D012117:Resins, Synthetic; C005044:hydroxyethyl methacrylate