title: Sonodynamically induced cell damage and membrane lipid peroxidation by novel porphyrin derivative, DCPH-P-Na(I).
abstract: BACKGROUND
  Ultrasonically induced cell damage and active oxygen generation with a novel porphyrin derivative DCPH-P-Na(I), were compared in the same in vitro insonation setup.
  
  
  METHODS
  Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of DCPH-P-Na(I). Cell viability was determined with the trypan blue exclusion test. Lipid peroxidation in cell membranes was estimated by measuring the amount of reactive substance produced immediately following the addition of thiobarbituric acid.
  
  
  RESULTS
  Significant enhancement of the rates of both ultrasonically induced cell damage and lipid peroxidation was observed in the presence of 2-8 muM DCPH-P-Na(I). Both rates correlated very well.
  
  
  CONCLUSIONS
  The enhancement of both rates with DCPH-P-Na(I) was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of DCPH-P-Na(I).
authors.affiliation: Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan. n.yumita@hamayaku.ac.jp
authors.forename: Nagahiko
authors.initials: N
authors.lastname: Yumita
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Iwase
authors.forename: Koji
authors.initials: K
authors.lastname: Nishi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ikeda
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Umemura
authors.forename: Isao
authors.initials: I
authors.lastname: Sakata
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Momose
mesh_terms: D000818:Animals; D016166:Free Radical Scavengers / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D011166:Porphyrins / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000378:metabolism; D014464:Ultrasonic Therapy
chemical_list: C521512:13,17-bis(1-carboxyethyl)-8-(2-(2,4-dichlorophenylhydrazono)ethylidene)-3-ethenyl-7-hydroxy-2,7,12,18-tetramethylchlorin; D016166:Free Radical Scavengers; D008563:Membrane Lipids; D011166:Porphyrins; D008353:Mannitol; D006639:Histidine