Sonodynamically induced cell damage and membrane lipid peroxidation by novel porphyrin derivative, DCPH-P-Na(I).
BACKGROUND
Ultrasonically induced cell damage and active oxygen generation with a novel porphyrin derivative DCPH-P-Na(I), were compared in the same in vitro insonation setup.


METHODS
Sarcoma 180 cells suspended in air-saturated PBS were exposed to ultrasound at 2 MHz for up to 60 s in the presence and absence of DCPH-P-Na(I). Cell viability was determined with the trypan blue exclusion test. Lipid peroxidation in cell membranes was estimated by measuring the amount of reactive substance produced immediately following the addition of thiobarbituric acid.


RESULTS
Significant enhancement of the rates of both ultrasonically induced cell damage and lipid peroxidation was observed in the presence of 2-8 muM DCPH-P-Na(I). Both rates correlated very well.


CONCLUSIONS
The enhancement of both rates with DCPH-P-Na(I) was suppressed by 10 mM histidine. These results suggest that ultrasonically generated active oxygen plays a primary role in the ultrasonically induced cell damage in the presence of DCPH-P-Na(I).
Yumita
Nagahiko
N
Yokohama College of Pharmacy, 601, Matano-cho, Totsuka-ku, Yokohama, Kanagawa 245-0066, Japan. n.yumita@hamayaku.ac.jp
Iwase
Yumiko
Y
Nishi
Koji
K
Ikeda
Toshihiko
T
Umemura
Shin-Ichiro
S
Sakata
Isao
I
Momose
Yasunori
Y
D000818:Animals; D016166:Free Radical Scavengers / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D011166:Porphyrins / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000378:metabolism; D014464:Ultrasonic Therapy
C521512:13,17-bis(1-carboxyethyl)-8-(2-(2,4-dichlorophenylhydrazono)ethylidene)-3-ethenyl-7-hydroxy-2,7,12,18-tetramethylchlorin; D016166:Free Radical Scavengers; D008563:Membrane Lipids; D011166:Porphyrins; D008353:Mannitol; D006639:Histidine