title: Characterization of the AP-1 μ1A and μ1B adaptins in zebrafish (Danio rerio).
abstract: Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins γ1, β1, μ1, σ1. Mammalia express μ1A ubiquitously and isoform μ1B in polarized epithelia. Mouse γ1 or μ1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated μ1A and μ1B from Danio rerio. Analysis of μ1A and μ1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. μ1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. μ1B is not expressed in murine liver, indicating loss of μ1B expression and establishment of alternative sorting mechanisms during mammalian development.
authors.affiliation: Department of Biomedical Sciences and Biotechnology, Unit of Biochemistry and Unit of Biology (GB), University of Brescia, Brescia, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Zizioli
authors.forename: Elena
authors.initials: E
authors.lastname: Forlanelli
authors.forename: Michela
authors.initials: M
authors.lastname: Guarienti
authors.forename: Stefania
authors.initials: S
authors.lastname: Nicoli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fanzani
authors.forename: Roberto
authors.initials: R
authors.lastname: Bresciani
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borsani
authors.forename: Augusto
authors.initials: A
authors.lastname: Preti
authors.forename: Franco
authors.initials: F
authors.lastname: Cotelli
authors.forename: Peter
authors.initials: P
authors.lastname: Schu
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000235:genetics / Q000378:metabolism*; D033983:Adaptor Protein Complex mu Subunits / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D016678:Genome; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology*; D010641:Phenotype; D010802:Phylogeny; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014018:Tissue Distribution; D015027:Zebrafish / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics / Q000254:growth & development*; D029961:Zebrafish Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D033961:Adaptor Protein Complex 1; D033983:Adaptor Protein Complex mu Subunits; D020033:Protein Isoforms; D029961:Zebrafish Proteins