title: 1H-HRMAS NMR study of smoked Atlantic salmon (Salmo salar).
abstract: High-resolution magic angle spinning (HRMAS) NMR spectroscopic data of smoked Atlantic salmon (Salmo salar) were fully assigned by combination of one- and two-dimensional-HRMAS experiments. Complete representative spectra, obtained after few minutes of analysis time, revealed a large number of minor and major compounds in the sample. The methodology is limited by the low sensitivity of NMR, and therefore HRMAS only enables the determination of the most relevant components. These were fatty acids (FAs), carbohydrates, nucleoside derivatives, osmolytes, amino acids, dipeptides and organic acids. For the first time, spectra were resolved sufficiently to allow semiquantitative determination in intact muscle of the highly polyunsaturated FA 22:6 omega-3. Additionally, the feasibility of (1)H-HRMAS NMR metabolite profiling was tested to identify some bioactive compounds during storage. This profiling was carried out by the non-destructive and direct analysis (i.e. without requiring sample preparation and multiple step procedures) of intact salmon muscle. The proposed procedure can be applied to a large number of samples with high throughput due to the short time of analysis and quick evaluation of the data.
authors.affiliation: CAI-RMN, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Castejón
authors.forename: Palmira
authors.initials: P
authors.lastname: Villa
authors.forename: Marta M
authors.initials: MM
authors.lastname: Calvo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Santa-María
authors.forename: Marta
authors.initials: M
authors.lastname: Herraiz
authors.forename: Antonio
authors.initials: A
authors.lastname: Herrera
mesh_terms: D000143:Acids / Q000032:analysis; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D004151:Dipeptides / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis / Q000737:chemistry; D009995:Osmosis; D011522:Protons; D012015:Reference Standards; D020317:Salmo salar; D017747:Seafood / Q000032:analysis*; D012906:Smoke / Q000032:analysis
chemical_list: D000143:Acids; D000596:Amino Acids; D002241:Carbohydrates; D004151:Dipeptides; D005227:Fatty Acids; D009705:Nucleosides; D011522:Protons; D012906:Smoke