Transcriptome analysis of Hpa1Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance.
The transcriptome profile in leaves and roots of the transgenic cotton line T-34 expressing hpa1(Xoo) from Xanthomonas oryzae pv. oryzae was analysed using a customized 12k cotton cDNA microarray. A total of 530 cDNA transcripts involved in 34 pathways were differentially expressed in the transgenic line T-34, in which 123 differentially expressed genes were related to the cotton defence responses including the hypersensitive reaction, defence responses associated with the recognition of pathogen-derived elicitors, and defence signalling pathways mediated by salicylic acid, jasmonic acid, ethylene, auxin, abscicic acid, and Ca(2+). Furthermore, transcripts encoding various leucine-rich protein kinases and mitogen-activated protein kinases were up-regulated in the transgenic line T-34 and expression of transcripts related to the energy producing and consuming pathway was also increased, which suggested that the enhanced metabolism related to the host defence response in the transgenic line T-34 imposed an increased energy demand on the transgenic plant.
Miao
Weiguo
W
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
Wang
Xiben
X
Song
Congfeng
C
Wang
Yu
Y
Ren
Yonghong
Y
Wang
Jinsheng
J
D001426:Bacterial Proteins / Q000378:metabolism*; D016000:Cluster Analysis; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D003368:Gossypium / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D007113:Immunity, Innate / Q000235:genetics*; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D014170:Transformation, Genetic; D014974:Xanthomonas / Q000378:metabolism
D001426:Bacterial Proteins; D012333:RNA, Messenger