A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor.
An adherently growing MDCK cell line was adapted in a two-step process in a fully defined medium and in suspension. The resulting MDCK.SUS2 cells were subsequently evaluated for their potential as host cells for influenza vaccine production in two lab-scale bioreactors (wave and stirred-tank). Cell concentrations up to 2.3 x 10(6)cells/mL were obtained after 96 h, which is slightly higher than cell concentrations obtained with adherent MDCK cells cultivated on microcarriers (2g/L). Infections with influenza A/PR/8/34 and B/Malaysia resulted in high virus titers (2.90 and 2.75 log HA units/100 microL, respectively). The monitoring of extracellular metabolites, including amino acids, revealed a change in some of the metabolite consumption or release profiles, which indicates changes in metabolism during the adaptation process. Overall, the MDCK.SUS2 cell line represents a new cell substrate for a robust influenza vaccine production in a fully defined process.
Lohr
V
V
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Sandtorstr. 1, 39106 Magdeburg, Germany. lohr@mpi-magdeburg.mpg.de
Genzel
Y
Y
Behrendt
I
I
Scharfenberg
K
K
Reichl
U
U
D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D003470:Culture Media; D004285:Dogs; D009975:Orthomyxoviridae / Q000254:growth & development; D014776:Virus Cultivation
D003470:Culture Media