Hfq regulates anti-oxidative ability in Vibrio parahaemolyticus.
Hfq plays a fundamental role in bacterial cell physiology. It can stimulate or repress the expression of certain target genes, and there is a possibility that Hfq regulates the oxidative stress response. However, how Hfq functions that in Vibrio parahaemolyticus remains speculative. In this paper, we explain the functions Hfq plays in V. parahaemolyticus in the gene expression of superoxide dismutase gene and catalase gene, comparing the hfq deletion mutant strain to the parental strain. The results show that the hfq deletion mutant V. parahaemolyticus has a stronger ability to resist H(2)O(2). Superoxide dismutase (SOD) and catalase (CAT) activities in the hfq deletion mutant were remarkably higher than in the parental strain. Genetic experiments indicated that the gene expression of sod and kat was up-regulated in the mutant strain. These results indicate that Hfq down-regulates CAT and SOD activity, and Hfq is associated with the oxidative stress response.
Su
Zehong
Z
Department of Preventive Environment and Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho 3-18-15, Tokushima City, Tokushima, Japan.
Nakano
Masayuki
M
Koga
Tetsuro
T
Lian
Xin
X
Hamamoto
Akiko
A
Shimohata
Takaaki
T
Harada
Yumi
Y
Mawatari
Kazuaki
K
Harada
Nagakatsu
N
Akutagawa
Masatake
M
Nakaya
Yutaka
Y
Takahashi
Akira
A
D002374:Catalase / Q000235:genetics / Q000378:metabolism; D035001:Host Factor 1 Protein / Q000502:physiology*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
D035001:Host Factor 1 Protein; D002374:Catalase; D013482:Superoxide Dismutase