Strain-promoted double-click reaction for chemical modification of azido-biomolecules.
The strain-promoted "double-click" (SPDC) reaction using Sondheimer diyne, a novel convergent method conjugating three molecules spontaneously, has enabled us to readily modify an azido-biomolecule with a small reporter azido-molecule.
Kii
Isao
I
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. ikii@bio.titech.ac.jp
Shiraishi
Akira
A
Hiramatsu
Toshiyuki
T
Matsushita
Takeshi
T
Uekusa
Hidehiro
H
Yoshida
Suguru
S
Yamamoto
Makoto
M
Kudo
Akira
A
Hagiwara
Masatoshi
M
Hosoya
Takamitsu
T
D001386:Azides / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D002460:Cell Line; D057930:Click Chemistry / Q000379:methods*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D001386:Azides; D002241:Carbohydrates; D005456:Fluorescent Dyes; D008055:Lipids; D009711:Nucleotides; D011506:Proteins