Aspergillus DNA contamination in blood collection tubes.
Fungal polymerase chain reaction (PCR)-based diagnostic methods are at risk for contamination. Sample collection containers were investigated for fungal DNA contamination using real-time PCR assays. Up to 18% of blood collection tubes were contaminated with fungal DNA, probably Aspergillus fumigatus. Lower proportions of contamination in other vessels were observed.
Harrison
Elizabeth
E
Manchester Academic Health Science Centre, University Hospital of South Manchester, The University of Manchester, Southmoor Road, Manchester M23 9LT, United Kingdom. liz.harrison@manchester.ac.uk
Stalhberger
Thomas
T
Whelan
Ruth
R
Sugrue
Michele
M
Wingard
John R
JR
Alexander
Barbara D
BD
Follett
Sarah A
SA
Bowyer
Paul
P
Denning
David W
DW
D001230:Aspergillus / Q000235:genetics*; D001769:Blood / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D013048:Specimen Handling
D004271:DNA, Fungal