title: [Culture and polymerase chain reaction of Helicobacter pylori from rectal and terminal ileal fluid after polyethylene glycol (colyte) ingestion in healthy adults with positive urea breath test].
abstract: OBJECTIVE
  Helicobacter pylori (H. pylori) transmission route is not yet clearly understood. Isolating H. pylori from stool, saliva, and vomitus is very difficult. However, H. pylori could be cultured from feces in the setting of rapid gastrointestinal tract transit. The aim of this study was to isolate H. pylori by culture and PCR in the rectum and terminal ileum during colonoscopy.
  
  
  METHODS
  Twenty subjects with positive UBT (urea breath test) were included. We performed polymerase chain reaction (PCR) test and culture of H. pylori with the rectal fluid and terminal ileal fluid during colonoscopy.
  
  
  RESULTS
  H. pylori was cultured with rectal fluid from 9 (45.0%) of 20 subjects and with ileal fluid from 11 (55.0%) of 20 subjects. H. pylori was a little more frequently cultured from the terminal ileal fluid than the rectal fluid without statistical significance (p>0.05). PCR test detected flaA (16/20, 80.0% and 17/20, 85.0%), 16S rRNA gene (16/20, 80.0% and 17/20, 85.0%), cagA (10/20, 50.0% and 12/20, 60.0%), and ureC (9/20, 45% and 11/20, 54.5%) from the rectal fluid and the terminal ileal fluid, respectively. The specificity and sensitivity of ureC were 100%.
  
  
  CONCLUSIONS
  H. pylori could be cultured from the rectal fluid and terminal ileal fluid in the setting of rapid gastrointestinal tract transit. These results suggest of fecal-oral transmission of H. pylori.
authors.affiliation: Department of Internal Medicine, Saint Carollo Hosipital, Suncheon, Korea.
authors.forename: Do Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Hong Myong
authors.initials: HM
authors.lastname: Jung
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Hwang
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Ahn
authors.forename: Jang Sik
authors.initials: JS
authors.lastname: Mun
authors.forename: Bo Hyun
authors.initials: BH
authors.lastname: Myoung
authors.forename: Hyeuk
authors.initials: H
authors.lastname: Park
authors.forename: Eun Joo
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Im
authors.forename: Hyun Min
authors.initials: HM
authors.lastname: Oh
authors.forename: Hui Yeong
authors.initials: HY
authors.lastname: Jeong
authors.forename: Chul
authors.initials: C
authors.lastname: Park
authors.forename: Hyung Rag
authors.initials: HR
authors.lastname: Kim
authors.forename: Eun Hae
authors.initials: EH
authors.lastname: Cho
authors.forename: Ho Dong
authors.initials: HD
authors.lastname: Kim
authors.forename: Young Do
authors.initials: YD
authors.lastname: Jun
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001944:Breath Tests; D004573:Electrolytes / Q000008:administration & dosage; D005243:Feces / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000635:transmission; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007082:Ileum / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000008:administration & dosage; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014508:Urea / Q000032:analysis; D014510:Urease / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004573:Electrolytes; C033608:Golytely; D012336:RNA, Ribosomal, 16S; C080866:cagA protein, Helicobacter pylori; D011092:Polyethylene Glycols; D014508:Urea; D014510:Urease