New steroidal glycosides isolated as CDL inhibitors of activated platelets.
Three new compounds were isolated from the dried bulbs of Allium macrostemon Bunge. Their structures were elucidated from their spectral data as (25R)-26-O-beta-D-glucopyranosyl-5alpha-furostane-3beta,12beta,22,26-tetraol-3-O-beta-D-glucopyranos-yl (1-->2) [beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl (1-->4)-beta-D-galactopyranoside (1), (25R)-26-O-beta-D-glucopyranosyl-5alpha-furostane-3beta,12alpha,22,26-tetraol-3-O-beta-D-glucopyranosyl (1-->2) [beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl (1-->4)-beta-D-galacto- pyranoside (2) and (25R)-26-O-beta-D-glucopyranosyl-5beta-furostane-3beta,12alpha,22,26-tetraol-3-O-beta-D-glucopyranosyl (1-->2)-beta-D-galactopyranoside (3), respectively. The inhibition effect of all compounds on CD40 ligand (CD40L) expression on the membrane of activated platelets stimulated by ADP was tested. Compounds 1 and 2 exhibited significant inhibitory activities in a dose dependent manner (P < 0.05), suggesting their potential application as CD40L inhibitors.
Chen
Haifeng
H
Institute for Biomedical Research, Xiamen University, Xiamen 361005, China. haifeng@xmu.edu.cn
Ou
Wenchao
W
Wang
Guanghui
G
Wang
Naili
N
Zhang
Linnan
L
Yao
Xinsheng
X
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000490:Allium / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects; D023201:CD40 Ligand / Q000037:antagonists & inhibitors*; D002236:Carbohydrate Conformation; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D015539:Platelet Activation / Q000187:drug effects*; D013057:Spectrum Analysis; D013256:Steroids
D006027:Glycosides; D013256:Steroids; D023201:CD40 Ligand; D000244:Adenosine Diphosphate