The association of -262C/T polymorphism in the catalase gene and delayed graft function of kidney allografts.
BACKGROUND
Catalase is an intracellular antioxidant enzyme that is mainly located in cellular peroxisomes and in the cytosol. This enzyme plays a significant role in the development of tolerance to oxidative stress in the adaptive response of cells and tissues. The aim of the present study was to examine the association between the -262C/T polymorphism in the catalase gene and delayed graft function (DGF), acute rejection and chronic allograft nephropathy of kidney allografts.


METHODS
One hundred eighty-seven recipients of first renal transplants were included in the study. The histories of the patients were analysed regarding DGF, acute rejection and chronic allograft nephropathy. The polymorphism -262C/T in the catalase gene was analysed using the polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) method.


RESULTS
The risk of DGF was significantly lower in T allele carriers compared with CC homozygotes: odds ratio = 0.34, 95% confidence interval = 0.17-0.67, P = 0.001. There were no statistically significant associations between the studied polymorphism and acute rejection or chronic allograft nephropathy.


CONCLUSIONS
The results of this study suggest that -262C/T polymorphism in the catalase gene is associated with DGF in kidney allograft recipients.
Dutkiewicz
Grazyna
G
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
Domanski
Leszek
L
Binczak-Kuleta
Agnieszka
A
Pawlik
Andrzej
A
Safranow
Krzysztof
K
Ciechanowicz
Andrzej
A
Dziedziejko
Violetta
V
Ciechanowski
Kazimierz
K
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D002374:Catalase / Q000235:genetics*; D051799:Delayed Graft Function / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006084:Graft Rejection / Q000201:enzymology / Q000235:genetics; D006085:Graft Survival / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D011044:Poland; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018570:Risk Assessment; D012307:Risk Factors; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D055815:Young Adult
D002374:Catalase