title: Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method.
abstract: A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of 350 samples (29.4%) by the original CMV LAMP procedure and 148 of 350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.
authors.affiliation: Department of Pharmacy, Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Suzuki
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Enomoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yano
authors.forename: Fumio
authors.initials: F
authors.lastname: Maruyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Emi
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral