title: Increase of reactive oxygen species by desferrioxamine during experimental Chagas' disease.
abstract: Oxidative stress is common in inflammatory processes associated with many diseases including Chagas' disease. The aim of the present study was to evaluate, in a murine model, biomarkers of oxidative stress together with components of the antioxidant system in order to provide an overview of the mechanism of action of the iron chelator desferrioxamine (DFO). The study population comprised 48 male Swiss mice, half of which were treated daily by intraperitoneal injection of DFO over a 35-day period, while half were administered sterile water in a similar manner. On the 14th day of the experiment, 12 DFO-treated mice and an equal number of untreated mice were experimentally infected with Trypanosoma cruzi. Serum concentrations of nitric oxide and superoxide dismutase and hepatic levels of total glutathione, thiobarbituric acid reactive species and protein carbonyl, were determined on days 0, 7, 14 and 21 post-infection. The results obtained revealed that DFO enhances antioxidant activity in the host but also increases oxidative stress, indicating that the mode of action of the drug involves a positive contribution to the host together with an effect that is not beneficial to the parasite.
authors.affiliation: Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil.
authors.forename: Amanda Fortes
authors.initials: AF
authors.lastname: Francisco
authors.forename: Paula Melo
authors.initials: PM
authors.lastname: de Abreu Vieira
authors.forename: Jerusa Marilda
authors.initials: JM
authors.lastname: Arantes
authors.forename: Maisa
authors.initials: M
authors.lastname: Silva
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Pedrosa
authors.forename: Silvana Maria
authors.initials: SM
authors.lastname: Elói-Santos
authors.forename: Olindo Assis
authors.initials: OA
authors.lastname: Martins-Filho
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: Márcio Sobreira Silva
authors.initials: MS
authors.lastname: Araújo
authors.forename: Washington Luiz
authors.initials: WL
authors.lastname: Tafuri
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D014355:Chagas Disease / Q000188:drug therapy* / Q000378:metabolism* / Q000469:parasitology; D003676:Deferoxamine / Q000494:pharmacology*; D004195:Disease Models, Animal; D007502:Iron Chelating Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017262:Siderophores / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000502:physiology
chemical_list: D000975:Antioxidants; D007502:Iron Chelating Agents; D017382:Reactive Oxygen Species; D017262:Siderophores; D013482:Superoxide Dismutase; D003676:Deferoxamine