title: CfLGBP, a pattern recognition receptor in Chlamys farreri involved in the immune response against various bacteria.
abstract: Lipopolysaccharide and beta-1, 3-glucan binding protein (LGBP) is a kind of pattern recognition receptor, which can recognize and bind LPS and beta-1, 3-glucan, and plays curial roles in the innate immune defense against Gram-negative bacteria and fungi. In this study, the functions of LGBP from Zhikong scallop Chlamys farreri performed in innate immunity were analyzed. Firstly, the mRNA expression of CfLGBP in hemocytes toward three typical PAMPS stimulation was examined by realtime PCR. It was up-regulated extremely (P < 0.01) post stimulation of LPS and beta-glucan, and also exhibited a moderate up-regulation (P < 0.01) after PGN injection. Further PAMPs binding assay with the polyclonal antibody specific for CfLGBP proved that the recombinant CfLGBP (designated as rCfLGBP) could bind not only LPS and beta-glucan, but also PGN in vitro. More importantly, rCfLGBP exhibited obvious agglutination activity towards Gram-negative bacteria Escherichia coli, Gram-positive bacteria Bacillus subtilis and fungi Pichia pastoris. Taking the results of immunofluorescence assay into account, which displayed CfLGBP was expressed specifically in the immune cells (hemocytes) and vulnerable organ (gill and mantle), we believed that LGBP in C. farreri, serving as a multi-functional PRR, not only involved in the immune response against Gram-negative and fungi as LGBP in other invertebrates, but also played significant role in the event of anti-Gram-positive bacteria infection. As the first functional research of LGBP in mollusks, our study provided new implication into the innate immune defense mechanisms of C. farreri and mollusks.
authors.affiliation: The Key laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd, Qingdao 266071, China.
authors.forename: Jialong
authors.initials: J
authors.lastname: Yang
authors.forename: Limei
authors.initials: L
authors.lastname: Qiu
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wei
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Linsheng
authors.initials: L
authors.lastname: Song
mesh_terms: D000371:Agglutination / Q000502:physiology; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D019085:Fluorescent Antibody Technique, Direct; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006434:Hemocytes / Q000378:metabolism; D007113:Immunity, Innate / Q000276:immunology*; D037102:Lectins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D049897:Pectinidae / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D010843:Pichia / Q000378:metabolism; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047071:beta-Glucans / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D037102:Lectins; D008070:Lipopolysaccharides; D051192:Receptors, Pattern Recognition; D047071:beta-Glucans