title: Differential profiles of c-fos expression within primary cultures of mouse cerebellar granule cells stimulated with the excitatory amino acid l-glutamate: Correlation with excitotoxic outcome and derivation of a predictive index.
abstract: Exposure of mouse cerebellar granule cells to l-glutamate (Glu) at low non-excitotoxic concentrations results in a transient expression of the c-fos proto-oncogene. Higher excitotoxic concentrations of Glu result in a change in the temporal profile of c-fos expression to a delayed but sustained response. We have derived a ratio for the steady-state levels of c-fos mRNA after 30 min and 240 min of exposure to Glu, which suggests that a 240 min/30 min ratio of greater than 1 correlates with excitotoxicity (as measured by elevated LDH release), whereas a ratio of less than 1 correlates with a non-excitotoxic outcome.
authors.affiliation: Immunotoxicology Department, BIBRA International, Woodmansterne Road, Carshalton, Surrey SM5 4DS UK.
authors.forename: C
authors.initials: C
authors.lastname: Malcolm
authors.forename: R
authors.initials: R
authors.lastname: Griffiths
authors.forename: P
authors.initials: P
authors.lastname: Rumsby
authors.forename: C
authors.initials: C
authors.lastname: Meredith