Rapid changes in plasma cortisol, osmolality, and respiration in response to salinity stress in tilapia (Oreochromis mossambicus).
We elucidated a time course for cortisol release in tilapia as it corresponds to changes in plasma osmolytes and respiration. Following exposure of freshwater (FW) tilapia to 25 per thousand seawater (SW), we measured plasma osmolality, [Na(+)], [K(+)], [Cl(-)], hematocrit, cortisol concentration, oxygen-consumption rate (MO2), and ventilation frequency over 5days and compared them to FW control fish. Cortisol increased rapidly by 3h and remained elevated for 3days. Plasma osmolality, [Na(+)], and [Cl(-)] were elevated at 6-8h, peaked 24h following SW exposure, and then decreased to near-FW levels by 3days. MO2 increased at 24h post-SW exposure relative to FW, while ventilation frequency increased by 3h. Overall, we interpret changes in cortisol as resulting from a change in salinity, in contrast to changes in plasma solute concentrations that could be due to adjustments resulting from the fish's cortisol response as it faces osmoregulatory distress. Increases in oxygen-consumption rate at 24h and ventilation frequency at 3h are likely as a result of the cellular stress response occurring during salinity stress. No significant changes in blood hematocrit were observed, which suggests that tilapia are capable of rapidly counteracting dehydration during acute hyperosmotic stress.
Kammerer
Brittany D
BD
Physiological Genomics Group, Department of Animal Science, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. Brittany.Kammerer@oregonstate.edu
Cech
Joseph J
JJ
Kültz
Dietmar
D
D000818:Animals; D002712:Chlorides / Q000097:blood; D005618:Fresh Water; D006400:Hematocrit; D006854:Hydrocortisone / Q000097:blood*; D009994:Osmolar Concentration; D010101:Oxygen Consumption; D012119:Respiration / Q000187:drug effects*; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology*; D012623:Seawater; D012964:Sodium / Q000097:blood; D013312:Stress, Physiological / Q000502:physiology*; D017210:Tilapia / Q000097:blood*; D014882:Water-Electrolyte Balance / Q000502:physiology
D002712:Chlorides; D012964:Sodium; D006854:Hydrocortisone