Identification of the MNN3 gene of Saccharomyces cerevisiae.
The MNN3 gene of Saccharomyces cerevisiae has been identified as a synonym of VPS74. We have compared phenotype characteristics of the original mnn3 mutant, including low dye binding phenotype, size of external invertase, clump formation, and sodium orthovanadate resistance and found these to be identical to those shown by vps74Δ. Mating of both haploid strains resulted in non-complementation of mutant phenotypes. Finally, a vector containing wild-type VPS74 complemented the defects of both vps74Δ and mnn3. This work completes the identification of the entire collection of genes that are defective in mnn mutants. In addition, we have identified the mnn3 mutation by sequencing the VPS74 gene from the original mnn3 strain. We found a single amino acid change of Arg97 to Cys. This unique alteration seems to be sufficient to account for the phenotype of mnn3.
Corbacho
Isaac
I
Department of Biomedical Sciences, Microbiology, University of Extremadura, 06006 Badajoz, Spain.
Olivero
Isabel
I
Hernández
Luis M
LM
D002352:Carrier Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006238:Haploidy; D009154:Mutation; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; C528905:Vps74 protein, S cerevisiae