Increased Siglec-1 expression in monocytes of patients with primary biliary cirrhosis.
OBJECTIVE
To evaluate Siglec-1 protein (CD169) and mRNA levels in peripheral blood monocytes of patients with primary biliary cirrhosis (PBC) and investigate its role in PBC pathogenesis by looking for correlations between Siglec-1 expression and key PBC associated biochemical indices.


METHODS
FACS analysis was used to identify the percentage of peripheral blood monocytes positive for both CD14 and Siglec-1 in (a) 45 PBC patients, (b) 40 patients with liver cirrhosis after hepatitis B infection and (c) 36 healthy controls. Siglec-1 mRNA was measured by real-time RT-PCR and serum biomarkers by routine biochemistry.


RESULTS
The percentage of CD14-Siglec-1 double positive cells was significantly higher (p< 0.01) in PBC patients than in healthy controls or cirrhosis post-hepatitis patients (13.68 +/- 2.44%, 1.0 +/- 0.2 %, and 4.1 +/- 0.5 %, respectively). Siglec-1 mRNA expression in the PBC group was 3.42 times higher than in healthy controls (p < 0.01).


CONCLUSIONS
We investigated the role of Siglec-1 in PBC by assessing its expression in mononuclear cells of PBC patients and levels of secreted cytokines in cell supernatants after Siglec-1 RNA interference. It is possible that elevated Siglec-1 expression in peripheral blood monocytes of PBC patients is correlated with monocyte-mediated inflammatory responses during the development of PBC.
Bao
Guangyu
G
Department of Laboratory Medicine, the First people's Hospital of Yangzhou, Jiangsu 225001, China.
Han
Zhijun
Z
Yan
Zihe
Z
Wang
Qihong
Q
Zhou
Ye
Y
Yao
Dingkang
D
Gu
Mingli
M
Chen
Bo
B
Chen
Sunxiao
S
Deng
Anmei
A
Zhong
Renqian
R
D000368:Aged; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D006509:Hepatitis B / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008105:Liver Cirrhosis, Biliary / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D063267:Sialic Acid Binding Ig-like Lectin 1; D015854:Up-Regulation
D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; D011971:Receptors, Immunologic; C571166:SIGLEC1 protein, human; D063267:Sialic Acid Binding Ig-like Lectin 1