title: Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.
abstract: Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.
authors.affiliation: Laboratory of Genetics and Gene Transfer, Core Research Laboratory, Istituto Toscano Tumori (ITT), AOU Careggi, Firenze, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Peruzzi
authors.forename: Martina
authors.initials: M
authors.lastname: Serra
authors.forename: Chiara
authors.initials: C
authors.lastname: Pescucci
authors.forename: Michela
authors.initials: M
authors.lastname: Sica
authors.forename: Sonia
authors.initials: S
authors.lastname: Lastraioli
authors.forename: Tommaso
authors.initials: T
authors.lastname: Rondelli
authors.forename: Simona
authors.initials: S
authors.lastname: Pedemonte
authors.forename: Antonio Maria
authors.initials: AM
authors.lastname: Risitano
authors.forename: Maria
authors.initials: M
authors.lastname: De Angioletti
authors.forename: Patrizia
authors.initials: P
authors.lastname: Piccioli
authors.forename: Rosario
authors.initials: R
authors.lastname: Notaro
mesh_terms: D000483:Alleles; D002460:Cell Line; D003176:Complement C3 / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D003176:Complement C3; D017931:DNA Primers