Targeting of Pex8p to the peroxisomal importomer.
Pex8p of Saccharomyces cerevisiae is located at the inner surface of the peroxisomal membrane and is essential for the assembly of the importomer, a multi-protein complex of the peroxisomal protein import machinery. By means of the yeast two-hybrid system as well as in vitro binding studies, we demonstrate that Pex8p is capable to interact with the PTS2-receptor Pex7p and the docking complex component Pex13p with its C-terminal SH3-domain providing the binding site. Analysis of the importomer composition of pex-mutants revealed that both PTS-receptors as well as the auxiliary proteins Pex18p and Pex21p are neither required for Pex8p association with the importomer nor for its function as an organizer of the peroxisomal protein import machinery.
Deckers
Markus
M
Abteilung für Systembiochemie, Institut für Physiologische Chemie, Medizinische Fakultät der Ruhr-Universität Bochum, D-44780 Bochum, Germany.
Emmrich
Kerstin
K
Girzalsky
Wolfgang
W
Awa
Wendy L
WL
Kunau
Wolf-Hubert
WH
Erdmann
Ralf
R
D002352:Carrier Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D000074401:Peroxins; D000074437:Peroxisomal Targeting Signal 2 Receptor; D011485:Protein Binding; D021381:Protein Transport / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C491105:PEX13 protein, S cerevisiae; C116845:PEX18 protein, S cerevisiae; C116846:PEX21 protein, S cerevisiae; D000074401:Peroxins; D000074437:Peroxisomal Targeting Signal 2 Receptor; C404515:Pex8 protein, S cerevisiae; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins