title: Comparison of the analytic sensitivities of a recombinant immunoblot assay and the radioimmune precipitation assay for the detection of antibodies to Trypanosoma cruzi in patients with Chagas disease.
abstract: The diagnosis of chronic Chagas disease usually is made by detecting antibodies to Trypanosoma cruzi, the protozoan parasite that causes this illness. A highly sensitive and specific immunoblot assay developed by us showed a higher analytic sensitivity than the radioimmune precipitation assay, which is used widely as a confirmatory test.
authors.affiliation: Emerging Pathogens and Infectious Diseases R&D, Abbott Diagnostics Division, Abbott Laboratories, IL 60064, USA. dinesh.shah@abbott.com
authors.forename: Dinesh O
authors.initials: DO
authors.lastname: Shah
authors.forename: Chi-Deu
authors.initials: CD
authors.lastname: Chang
authors.forename: Kevin Y
authors.initials: KY
authors.lastname: Cheng
authors.forename: Vince A
authors.initials: VA
authors.lastname: Salbilla
authors.forename: Neeraj
authors.initials: N
authors.lastname: Adya
authors.forename: Benedict A
authors.initials: BA
authors.lastname: Marchlewicz
authors.forename: Louis V
authors.initials: LV
authors.lastname: Kirchhoff
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D014355:Chagas Disease / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D011863:Radioimmunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan