Preparation of silver nanoparticles with antimicrobial activities and the researches of their biocompatibilities.
Silver nanoparticles were prepared by chemical reduction method using chitosan as stabilizer and ascorbic acid as reducing agent in this work. The silver/chitosan nanocomposites were characterized in terms of their particle sizes and morphology by using UV spectrophotometer, nano-grainsize analyzer, and transmission electron microscopy. Antibacterial activities of these nanocomposites were carried out for Staphylococcus aureus and Escherichia coli. The silver nanoparticles exhibited significantly inhibition capacity towards these bacteria. Detailed studies on the biocompatibility of the silver/chitosan nanocomposites were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell adhesion test. The results indicated that these silver/chitosan nanocomposites were benefit for the proliferation and adhesion of L-929 cells, and the biocompatibilities between the nanocomposites and the cells would become better with the culturing days. We anticipated that these silver/chitosan nanocomposites could be a promising candidate as coating material in biomedical engineering and food packing fields wherein antibacterial properties and biocompatibilities are crucial.
Cao
X L
XL
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, ChengDu, China.
Cheng
C
C
Ma
Y L
YL
Zhao
C S
CS
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000633:toxicity; D004926:Escherichia coli / Q000187:drug effects; D066298:In Vitro Techniques; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D051379:Mice; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D012834:Silver; D048271:Chitosan