Application of siRNA in targeting protein expression in kidney disease.
Although it is one of the major targeted organs by systemically administered siRNA, when compared to other tissues the kidney receives only moderate interest regarding therapeutic siRNA delivery. Here we review recent approaches to target renal protein expression under normal and pathological conditions. Experimental evidence to support the clinical relevance of siRNA administration in the treatment of renal disease is discussed. High-throughput screening using recently available genome-wide RNA interference libraries provides a new, powerful tool that can be applied to conventional and 3D in vitro culture models for lead finding or the identification of signal pathway involvement in renal disease.
Stokman
Geurt
G
Department of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Qin
Yu
Y
Rácz
Zsuzsanna
Z
Hamar
Peter
P
Price
Leo S
LS
D000818:Animals; D004195:Disease Models, Animal; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005822:Genetic Vectors; D006801:Humans; D007674:Kidney Diseases / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*
D004337:Drug Carriers; D011506:Proteins; D034741:RNA, Small Interfering