A novel chemoattractant lectin, karatoxin, from the dorsal spines of the small scorpionfish Hypodytes rubripinnis.
A chemoattractant lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, was isolated using a combination of affinity chromatography techniques. The glycoprotein, with a molecular mass of 110 kDa, is named Karatoxin. Karatoxin caused agglutination of rabbit erythrocytes. This agglutination was effectively inhibited by D-mannose. In addition, Karatoxin exhibited not only mitogenic activity in the presence of murine splenocytes, but also chemotactic activity in the presence of guinea-pig neutrophils and macrophages. Thus, Karatoxin appears to be a novel chemoattractant lectin. These results suggest that the redfin velvetfish Hypodytes rubripinnis may be a novel source of biologically active substances.
Shinohara
Mitsuko
M
Depatment of Pharmacology, Osaka Dental University, Hirakata, Japan. shino@cc.osaka-dent.ac.jp
Nagasaka
Kuniko
K
Nakagawa
Hideyuki
H
Edo
Kozue
K
Sakai
Hitomi
H
Kato
Koji
K
Iwaki
Futoshi
F
Ohura
Kiyoshi
K
Sakuraba
Haruhiko
H
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002846:Chromatography, Affinity; D005399:Fishes; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D011817:Rabbits
D006023:Glycoproteins; D037102:Lectins; C554065:karatoxin, Hypodytes rubripinnis