title: Layer-by-layer assembly sensitive electrochemical sensor for selectively probing L-histidine based on molecular imprinting sol-gel at functionalized indium tin oxide electrode.
abstract: A novel sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of L-histidine by combination of a molecular imprinting film and multi-walled carbon nanotubes (MWNTs). The sensor was fabricated onto an indium tin oxide (ITO) electrode via stepwise modification of MWNTs and a thin film of molecularly imprinted polymers (MIPs) via sol-gel technology. The introduced MWNTs exhibited noticeable enhancement on the sensitivity of the MIPs sensor. Meanwhile, the molecularly imprinted film displayed high sensitivity and excellent selectivity for the target molecule L-histidine. The proposed imprinted sensor was characterized by using scanning electron microscope (SEM) and electrochemical methods involving cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometric i-t curve. A linear ranging from 2.0 μmol L(-1) to 1.0 mmol L(-1) for the detection of L-histidine was observed with the detection limit of 5.8×10(-9) mol L(-1) for S/N=3. This imprinted electrochemical sensor was successfully employed to detect L-histidine in human blood serum.
authors.affiliation: College of Chemistry and Chemical Engineering, Jishou University, Hunan 416000, PR China. zhaohuizhang77@hotmail.com
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yufang
authors.initials: Y
authors.lastname: Hu
authors.forename: Huabin
authors.initials: H
authors.lastname: Zhang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Luo
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D003460:Crystallization / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006639:Histidine / Q000032:analysis*; D008839:Microelectrodes; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D017971:Tin Compounds; D006639:Histidine; C109984:indium tin oxide