Characterization of the envelope glycoproteins associated with infectious hepatitis C virus.
Hepatitis C is caused by an enveloped virus whose entry is mediated by two glycoproteins, namely, E1 and E2, which have been shown to assemble as a noncovalent heterodimer. Despite extensive research in the field of such an important human pathogen, hepatitis C virus (HCV) glycoproteins have only been studied so far in heterologous expression systems, and their organization at the surfaces of infectious virions has not yet been described. Here, we characterized the envelope glycoproteins associated with cell-cultured infectious virions and compared them with their prebudding counterparts. Viral particles were analyzed by ultracentrifugation, and the envelope glycoproteins were characterized by coimmunoprecipitation and receptor pulldown assays. Furthermore, their oligomeric state was determined by sedimentation through sucrose gradients and by separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions. In sucrose gradient analyses, HCV envelope glycoproteins were associated with fractions containing the most infectious viral particles. Importantly, besides maturation of some of their glycans, HCV envelope glycoproteins showed a dramatic change in their oligomeric state after incorporation into the viral particle. Indeed, virion-associated E1 and E2 envelope glycoproteins formed large covalent complexes stabilized by disulfide bridges, whereas the intracellular forms of these proteins assembled as noncovalent heterodimers. Furthermore, the virion-associated glycoprotein complexes were recognized by the large extracellular loop of CD81 as well as conformation-sensitive antibodies, indicating that these proteins are in a functional conformation. Overall, our study fills a gap in the description of HCV outer morphology and should guide further investigations into virus entry and assembly.
Vieyres
Gabrielle
G
Institut Pasteur de Lille, Center for Infection & Immunity of Lille, F-59019 Lille, France.
Thomas
Xavier
X
Descamps
Véronique
V
Duverlie
Gilles
G
Patel
Arvind H
AH
Dubuisson
Jean
J
D015703:Antigens, CD / Q000502:physiology; D002460:Cell Line; D004220:Disulfides / Q000737:chemistry; D006031:Glycosylation; D016174:Hepacivirus / Q000737:chemistry* / Q000472:pathogenicity / Q000502:physiology; D006493:Heparin / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000502:physiology; D006801:Humans; D008358:Mannose / Q000737:chemistry; D046912:Multiprotein Complexes / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011487:Protein Conformation; D055503:Protein Multimerization; D011991:Receptors, Virus / Q000502:physiology; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology
D015703:Antigens, CD; C554798:CD81 protein, human; D004220:Disulfides; C087035:E1 protein, Hepatitis C virus; D046912:Multiprotein Complexes; D011134:Polysaccharides; D011991:Receptors, Virus; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus; D006493:Heparin; D008358:Mannose