title: Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise.
abstract: High-intensity exercise shares similarities with acute phase responses of inflammatory diseases. We investigated the influences of acute exercise on inflammatory markers, plasma pentraxin3 (PTX3) and serum high-sensitive C-reactive protein (CRP) (hsCRP). Nine healthy male subjects (41 ± 3 years old) participated. Each subject performed three types of exercise; ergometer exercise at 70% workload of anaerobic threshold (AT) for 30 min (70% AT exercise), peak ergometer exercise (peak EX, 20 watt increase/min until fatigue) and resistance exercises of 70% 1 RM (70% RE) until exhaustion. We measured plasma PTX3, serum hsCRP, lactate, noradrenaline (NOR), white blood cells (WBC), interleukin-6 (IL-6) and myeloperoxidase (MPO), a marker of neutrophil degranulation. The effects of exercise on intracellular PTX3 and MPO in neutrophils were also investigated, by using flow cytometry analysis. Circulating PTX3 and hsCRP significantly increased immediately after 70% RE and peak EX, while they did not increase after 70% AT exercise. The exercise-induced fold increase in PTX3 and hsCRP relative to the resting level was positively correlated with the changes in WBC, NOR, lactate and MPO. The exercise-induced fold increase in IL-6 was positively correlated with that in NOR, but not with that in PTX3 and hsCRP. Neutrophils isolated immediately after 70% RE, but not 70% AT exercise, exhibited lower mean fluorescence for PTX3 and MPO than those from pre-exercise blood. These results provide the evidence that high-intensity exercises significantly increase circulatory PTX3 as well as hsCRP. The release from peripheral neutrophils is suggested to be involved in the exercise-induced plasma PTX3 increase.
authors.affiliation: Department of Ischemic Circulatory Physiology, KAATSU Training, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. masamasa@pb4.so-net.ne.jp
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakajima
authors.forename: Miwa
authors.initials: M
authors.lastname: Kurano
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Haruhito
authors.initials: H
authors.lastname: Takano
authors.forename: Haruko
authors.initials: H
authors.lastname: Iida
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Taira
authors.initials: T
authors.lastname: Fukuda
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Madarame
authors.forename: Kansei
authors.initials: K
authors.lastname: Uno
authors.forename: Kentaro
authors.initials: K
authors.lastname: Meguro
authors.forename: Taro
authors.initials: T
authors.lastname: Shiga
authors.forename: Mina
authors.initials: M
authors.lastname: Sagara
authors.forename: Taiji
authors.initials: T
authors.lastname: Nagata
authors.forename: Koji
authors.initials: K
authors.lastname: Maemura
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Hirata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yamasoba
authors.forename: Ryozo
authors.initials: R
authors.lastname: Nagai
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D016552:Ergometry; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000503:physiopathology*; D015850:Interleukin-6 / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000737:chemistry; D009638:Norepinephrine / Q000097:blood; D009195:Peroxidase / Q000097:blood; D055070:Resistance Training; D000683:Serum Amyloid P-Component / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D000099153:Pentraxins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D015850:Interleukin-6; D019344:Lactic Acid; D009638:Norepinephrine; D009195:Peroxidase; D000683:Serum Amyloid P-Component; D000099153:Pentraxins