Proteomic study of sera from patients with bladder cancer: usefulness of S100A8 and S100A9 proteins.
To effectively obtain tumour-specific markers, fractionated proteins obtained using reversed-phase high-performance liquid chromatography for patient-matched pre- and postoperative sera from bladder cancer patients were compared by two-dimensional gel electrophoresis. The usefulness of the identified proteins was confirmed immunohistochemically. S100A8 and S100A9 were identified as tumour-associated proteins. The increased immunoreactive expression of S100A8 protein was associated with bladder wall muscle invasion of the tumour and cancer-specific survival (p<0.05), and the increased immunoreactive expression of S100A9 protein was associated with the tumour grade (p<0.05). In addition, increased expressions of both proteins was associated with recurrence-free survival at a median follow-up of 32.9 months (both p<0.05). On multivariate analysis, the expression of S100A8 was a significant predictor of recurrence (p<0.05). These findings may help to identify biologically aggressive tumors and, thus, patients who might benefit from more intensive adjuvant therapy.
Minami
Sho
S
Department of Molecular Diagnosis, Kitasato University Graduate School of Medical Sciences, Sagamihara, Kanagawa 228-8555, Japan.
Sato
Yuichi
Y
Matsumoto
Toshihide
T
Kageyama
Taihei
T
Kawashima
Yusuke
Y
Yoshio
Kodera
K
Ishii
Jun-Ichiro
J
Matsumoto
Kazumasa
K
Nagashio
Ryo
R
Okayasu
Isao
I
D040501:Calgranulin A / Q000097:blood*; D040502:Calgranulin B / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D011379:Prognosis; D040901:Proteomics; D015203:Reproducibility of Results; D001749:Urinary Bladder Neoplasms / Q000097:blood* / Q000175:diagnosis
D040501:Calgranulin A; D040502:Calgranulin B