In vitro lens capsule model for investigation of posterior capsule opacification.
A modified dissection technique of donor eyes for investigating posterior capsule opacification (PCO) that preserves normal capsule and zonule architecture is described. The intact crystalline lens-zonule-ciliary body complex is dissected from the globe in one piece and pinned with 8 entomological pins through the ciliary body to a soft silicone ring with an internal diameter of 12.7 mm. The specimen is iridectomized and cataract extraction performed; the specimen is then placed in culture. The capsule is supported by the native zonules and suspended freely within culture medium. The entire capsular bag is visible, allowing observation of lens epithelial cell (LEC) growth. In 15 eyes of 13 donors, the mean time to LEC confluence was 10.4 days +/- 1.4 [SD]. This technique builds on previous capsular bag models, providing a more physiological model for observation of PCO in vitro.


BACKGROUND
No author has a financial or proprietary interest in any material or method mentioned.
Cleary
Georgia
G
Department of Ophthalmology, St. Thomas' Hospital, London SE1 7EH, United Kingdom.
Spalton
David J
DJ
Zhang
Jin-Jun
JJ
Marshall
John
J
D000368:Aged; D002386:Cataract / Q000473:pathology*; D004210:Dissection / Q000379:methods; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D007903:Lens Capsule, Crystalline / Q000473:pathology*; D008022:Ligaments / Q000473:pathology; D008858:Microscopy, Phase-Contrast; D008954:Models, Biological; D009924:Organ Culture Techniques; D013048:Specimen Handling; D014019:Tissue Donors