title: Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats.
abstract: This study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 10(6) CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization.
authors.affiliation: Department of Pediatric Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
authors.forename: Taís Elisabete
authors.initials: TE
authors.lastname: Crivelaro de Menezes
authors.forename: Alberto Carlos
authors.initials: AC
authors.lastname: Botazzo Delbem
authors.forename: Fernanda
authors.initials: F
authors.lastname: Lourenção Brighenti
authors.forename: Ana
authors.initials: A
authors.lastname: Cláudia Okamoto
authors.forename: Elerson
authors.initials: E
authors.lastname: Gaetti-Jardim
mesh_terms: D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D018441:Biofilms / Q000187:drug effects; D001938:Brazil; D002327:Cariostatic Agents / Q000009:adverse effects / Q000627:therapeutic use*; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects; D006244:Hardness / Q000187:drug effects; D008297:Male; D008519:Medicine, Traditional; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D031647:Psidium / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control
chemical_list: D002327:Cariostatic Agents; D010936:Plant Extracts