title: Characterisation of allergen-specific responses of IL-10-producing regulatory B cells (Br1) in Cow Milk Allergy.
abstract: CD19+CD5+ regulatory B cells regulate immune responses by producing IL-10. IL-10-producing regulatory B cell (Br1) responses by allergen stimulation were investigated in human food allergy. Six milk allergy patients and eight milk-tolerant subjects were selected according to DBPCFC. PBMCs were stimulated by casein in vitro and stained for intracellular IL-10 and apoptosis. In response to allergen stimulation, Br1 decreased from 26.2+/-18.3 to 15.5+/-8.9% (p=0.031, n=6) in the milk allergy group and increased from 15.4+/-9.0 to 23.7+/-11.2% (p=0.023, n=8) in the milk-tolerant group. Apoptotic non-IL-10-producing regulatory B cells increased from 21.8+/-9.3 to 38.0+/-16.1% (p=0.031, n=6) in the milk allergy group and unchanged from 28.8+/-13.8 to 28.0+/-15.0% (p=0.844, n=8) in the milk-tolerant group. Br1 may be involved in the immune tolerance of food allergies by producing IL-10 and simultaneously undergoing apoptosis in humans. The exact roles for Br1 in immune tolerance needs to be further investigated.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Joonyong
authors.initials: J
authors.lastname: Noh
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Geunwoong
authors.initials: G
authors.lastname: Noh
authors.forename: So Young
authors.initials: SY
authors.lastname: Bang
authors.forename: Hyuk Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Sunheui
authors.initials: S
authors.lastname: Cho
authors.forename: Sang Sun
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology; D000818:Animals; D018941:Antigens, CD19 / Q000096:biosynthesis; D017209:Apoptosis / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D018956:CD5 Antigens / Q000096:biosynthesis; D002364:Caseins / Q000276:immunology; D002417:Cattle; D002452:Cell Count; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D056747:Immunomodulation; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D016269:Milk Hypersensitivity / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology
chemical_list: D000485:Allergens; D018941:Antigens, CD19; D018956:CD5 Antigens; D002364:Caseins; D016753:Interleukin-10