The oocyte-specific transcription factor, Nobox, regulates the expression of Pad6, a peptidylarginine deiminase in the oocyte.
Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 (Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.
Choi
Mikyung
M
Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea.
Lee
Ok-Hee
OH
Jeon
Sanghyun
S
Park
Miseon
M
Lee
Dong Ryul
DR
Ko
Jeong-Jae
JJ
Yoon
Tae Ki
TK
Rajkovic
Aleksandar
A
Choi
Youngsok
Y
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D010053:Ovary / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D000080065:Protein-Arginine Deiminase Type 6; D000076342:Protein-Arginine Deiminases; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D017931:DNA Primers; D018398:Homeodomain Proteins; D006867:Hydrolases; D000080065:Protein-Arginine Deiminase Type 6; D000076342:Protein-Arginine Deiminases; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C456265:Og2x protein, mouse; C523190:PADI6 protein, mouse