title: [Dextran sedimentation for study of neutrophil polarization].
abstract: OBJECTIVE
  To determine the optimal method for separating neutrophils for studying neutrophil polarization.
  
  
  METHODS
  Human neutrophil was separated from healthy human peripheral blood by Percoll density gradient centrifugation and Dextran sedimentation. The cell polarization, purity and activity of the neutrophils were determined, and F-actin polymerization and [Ca2+]i were analyzed.
  
  
  RESULTS
  No significant difference was found in cell polarization, purity and activity of the human neutrophils separated by Dextran sedimentation and Percoll density gradient centrifugation (P>0.05), but F-actin polymerization was inhibited in PMNs separated by Dextran sedimentation, and the peak value of [Ca2+]i was decreased by 25% in PMNs separated by Dextran sedimentation compared to the cells separated by Percoll density gradient centrifugation.
  
  
  CONCLUSIONS
  Both Percoll density gradient centrifugation and Dextran sedimentation can be used for isolating human neutrophils to study cell polarization, but the former method allows better isolation. Dextran sedimentation can be considered when a large number of neutrophils need to be separated.
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China. luoyy1982@fimmu.com
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.forename: Chun-qing
authors.initials: CQ
authors.lastname: Cai
authors.forename: Xui-hua
authors.initials: XH
authors.lastname: Xie
authors.forename: Xin-wei
authors.initials: XW
authors.lastname: Chu
authors.forename: Qing-yi
authors.initials: QY
authors.lastname: Wu
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Meng
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D000199:Actins; D016764:Cell Polarity; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000166:cytology*; D011205:Povidone; D012822:Silicon Dioxide
chemical_list: D000199:Actins; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone