title: New steroidal glycosides isolated as CDL inhibitors of activated platelets.
abstract: Three new compounds were isolated from the dried bulbs of Allium macrostemon Bunge. Their structures were elucidated from their spectral data as (25R)-26-O-beta-D-glucopyranosyl-5alpha-furostane-3beta,12beta,22,26-tetraol-3-O-beta-D-glucopyranos-yl (1-->2) [beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl (1-->4)-beta-D-galactopyranoside (1), (25R)-26-O-beta-D-glucopyranosyl-5alpha-furostane-3beta,12alpha,22,26-tetraol-3-O-beta-D-glucopyranosyl (1-->2) [beta-D-glucopyranosyl (1-->3)]-beta-D-glucopyranosyl (1-->4)-beta-D-galacto- pyranoside (2) and (25R)-26-O-beta-D-glucopyranosyl-5beta-furostane-3beta,12alpha,22,26-tetraol-3-O-beta-D-glucopyranosyl (1-->2)-beta-D-galactopyranoside (3), respectively. The inhibition effect of all compounds on CD40 ligand (CD40L) expression on the membrane of activated platelets stimulated by ADP was tested. Compounds 1 and 2 exhibited significant inhibitory activities in a dose dependent manner (P < 0.05), suggesting their potential application as CD40L inhibitors.
authors.affiliation: Institute for Biomedical Research, Xiamen University, Xiamen 361005, China. haifeng@xmu.edu.cn
authors.forename: Haifeng
authors.initials: H
authors.lastname: Chen
authors.forename: Wenchao
authors.initials: W
authors.lastname: Ou
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wang
authors.forename: Naili
authors.initials: N
authors.lastname: Wang
authors.forename: Linnan
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000490:Allium / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects; D023201:CD40 Ligand / Q000037:antagonists & inhibitors*; D002236:Carbohydrate Conformation; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D015539:Platelet Activation / Q000187:drug effects*; D013057:Spectrum Analysis; D013256:Steroids
chemical_list: D006027:Glycosides; D013256:Steroids; D023201:CD40 Ligand; D000244:Adenosine Diphosphate