Simultaneous dual-tracer PET imaging of the rat brain and its application in the study of cerebral ischemia.
OBJECTIVE
This study evaluates the performance of simultaneous dual-tracer technique (SDTT) in static positron emission tomography (PET) studies using 2-deoxy-2-[¹⁸F]fluoro-D-glucose and [¹³N]ammonium as radiotracers.


METHODS
The effects of applying SDTT either to the reconstructed image or directly to the sinogram, different rebinning algorithms, total acquisition time, and frame duration were investigated; first, using a specific phantom and later using an in vivo application of the study of cerebral ischemia.


RESULTS
The best results were obtained using the image method with single-slice rebinning and a total acquisition time of at least 20 min. Frame duration did not affect SDTT performance. The method was also applied in rats with transient cerebral ischemia to simultaneously study cerebral blood flow and cerebral glucose metabolism.


CONCLUSIONS
The results encourage the use of SDTT as it has very good potential for examining two different biological processes at the same time utilising rodent PET scanners.
Figueiras
Francisca P
FP
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
Jiménez
Xavier
X
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
Pareto
Deborah
D
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
Gómez
Vanessa
V
CIC-BiomaGUNE, Unidad de Imagen, San Sebastián, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
Llop
Jordi
J
CIC-BiomaGUNE, Unidad de Imagen, San Sebastián, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
Herance
Raul
R
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
Rojas
Santiago
S
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
Gispert
Juan D
JD
Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain. jgispert@crccorp.es.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain. jgispert@crccorp.es.
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000473:pathology*; D002545:Brain Ischemia / Q000000981:diagnostic imaging*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D008297:Male; D019047:Phantoms, Imaging; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18