title: A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens.
abstract: A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.
authors.affiliation: Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chungli, 320, Taiwan.
authors.forename: Ya-Fen
authors.initials: YF
authors.lastname: Wang
authors.forename: How-Ran
authors.initials: HR
authors.lastname: Chao
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Wu
authors.forename: Chu-Han
authors.initials: CH
authors.lastname: Tseng
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Kuo
authors.forename: Tsui-Chun
authors.initials: TC
authors.lastname: Tsou
mesh_terms: D050156:Adipogenesis; D001681:Biological Assay / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000032:analysis*; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis*; D020025:Peroxisome Proliferators / Q000378:metabolism*; D020218:Response Elements
chemical_list: D004785:Environmental Pollutants; D020025:Peroxisome Proliferators; D008156:Luciferases