title: Development of fluoroapatite chromatography for the purification of monoclonal antibody.
abstract: Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.
authors.affiliation: Process R&D Applications, Life Science Group, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com
authors.forename: Paul K
authors.initials: PK
authors.lastname: Ng
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshitake
authors.forename: Mark A
authors.initials: MA
authors.lastname: Snyder
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001031:Apatites / Q000737:chemistry*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D001031:Apatites; D007074:Immunoglobulin G; D008670:Metals; C025105:fluorapatite