p53 codon 72 polymorphism association with head and neck squamous cell carcinoma.
p53 tumoral suppressor gene harbors a functional polymorphism which codes either arginine (Arg) or proline (Pro) in the protein p53 of codon 72. Such polymorphism has been associated with the development or prognosis of head and neck squamous cell carcinoma (HNSCC).


OBJECTIVE
we assessed codon 72 p53 allelic frequencies and genotypes in HNSCC Iranian patients.


METHODS
Case Study.


METHODS
a total of 132 HNSCC patients and 123 healthy controls were genotyped. DNA source was from mononuclear cells of the peripheral blood. DNA amplification was done by means of the allele-specific polymerase chain reaction.


RESULTS
genotypes and allele distribution were not significantly different between patients and controls. Moreover, no statistically significant association was found between the 72 and p53 codon tumor location, gender or age at the time of diagnosis. However, the Pro/Pro genotype was significantly increase in stage IV patients (30.8%) when compared to stages I-III of the disease (11.1%) (p=0.03), and a significantly higher percentage of patients with the Pro allele had and a risk increase in stage IV disease (OR=2.2, 95% CI=1.2-4.2, p=0.01).


CONCLUSIONS
data revealed that the p53 polymorphism do not impact the risk of HNSCC in Iranians, nonetheless, it can affect tumor progression to a higher tumor stage.
Mojtahedi
Zahra
Z
Shiraz Insttute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Hashemi
Seyed Basir
SB
Khademi
Bijan
B
Karimi
Morteza
M
Haghshenas
Mohammad Reza
MR
Fattahi
Mohammad Javad
MJ
Ghaderi
Abbas
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
D003062:Codon