title: Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones.
abstract: Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of Haemophilus influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants.
authors.affiliation: Pathogen Functional Genomics Resource Center (PFGRC), The J. Craig Venter Institute (JCVI), 9712 Medical Center Drive, Rockville, MD 20850, USA.
authors.forename: Leka
authors.initials: L
authors.lastname: Papazisi
authors.forename: Shashikala
authors.initials: S
authors.lastname: Ratnayake
authors.forename: Brian G
authors.initials: BG
authors.lastname: Remortel
authors.forename: Geoffrey R
authors.initials: GR
authors.lastname: Bock
authors.forename: Wei
authors.initials: W
authors.lastname: Liang
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Saeed
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Robert D
authors.initials: RD
authors.lastname: Fleischmann
authors.forename: Mogens
authors.initials: M
authors.lastname: Kilian
authors.forename: Scott N
authors.initials: SN
authors.lastname: Peterson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil; D055028:Comparative Genomic Hybridization; D005334:Fever / Q000382:microbiology*; D014644:Genetic Variation; D016680:Genome, Bacterial; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D011693:Purpura / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors