Morphological, morphometrical and ultrastructural characterization of Phrynops geoffroanus' (Testudines: Chelidae) blood cells, in different environments.
The aim of this study was to evaluate the formed elements in the periferical blood of two amostral groups of Phrynops geoffroanus: one from an urban environment under domestic sewage dumping, and another from a non-contaminated environment. Blood samples of 36 animals (females and males) were collected through cardiocentesis. Sixteen specimens were from the urban environment, and 20 were from a control environment. Samples of blood tissue were used for light microscopy analysis, and also for morphometric analysis of red blood cells. For the ultrastructural analysis, blood samples of 2 animals were used. The formed elements found, using morphological and ultrastructural analysis were: nucleated red blood cells; thrombocytes; neutrophils, lymphocytes, monocytes, basophils; eosinophils; heterophils, and azurophils. The morphometric analysis of all red blood cells parameters examinated in females showed a statistically significant difference, but in males just the nuclear area showed difference between the specimens of the two environments. The elements identified by light microscopy were elucidated by electron transmission microscopy. This P. geoffroanus study is the first one that makes a correlation between these environments and the description of turtle's blood cells, thus contributing to the identification of the hematological characteristics of this group.
Zago
Carlos Eduardo Saranz
CE
Departamento de Biologia, Centro de Estudos de Quelônios, UNESP-IBILCE-São José do Rio Preto, SP, Brazil.
da Silva
Tiago Lucena
TL
da Silva
Maria Isabel Afonso
MI
Venancio
Larissa Paola Rodrigues
LP
Mendonça
Priscila Pasqüetto
PP
Junior
Luiz Roberto Falleiros
LR
Vizotto
Luiz Dino
LD
Taboga
Sebastião Roberto
SR
Bonini-Domingos
Claudia Regina
CR
de Azeredo-Oliveira
Maria Tercília Vilela
MT
de Oliveira
Classius
C
D000818:Animals; D001699:Biometry; D001773:Blood Cells / Q000166:cytology* / Q000648:ultrastructure*; D043344:Chordata / Q000502:physiology*; D004781:Environmental Exposure; D005260:Female; D008297:Male; D008853:Microscopy; D014876:Water Pollution