title: Lymphocyte calcium influx characteristics and their modulation by Kv1.3 and IKCa1 channel inhibitors in healthy pregnancy and preeclampsia.
abstract: OBJECTIVE
  Calcium handling of T lymphocytes is altered in healthy pregnancy (HP) and preeclampsia (PE) compared to non-pregnant (non-P) women. We compared the activation-elicited calcium influx in T lymphocytes in HP, PE and non-P women and tested its alteration upon inhibition of Kv1.3 and IKCa1 potassium channels.
  
  
  METHODS
  The alteration of calcium influx was measured in major T-lymphocyte subsets of 9 non-P, HP and PE women with flow cytometry with or without treatment of cells with potassium channel inhibitors.
  
  
  RESULTS
  The elicited calcium response was lower in HP compared to non-P. In HP, calcium influx was sensitive to potassium channel inhibition in CD8 and Th1, but not in Th2 cells. In PE, calcium influx and its sensitivity to inhibition were comparable to non-P.
  
  
  CONCLUSIONS
  There is a characteristic pattern of calcium influx in T lymphocytes and its sensitivity to potassium channel inhibition in HP that is missing in PE, raising the notion that T-lymphocyte calcium handling may have a role in the characteristic immune status of HP.
authors.affiliation: First Department of Pediatrics, Semmelweis University, Budapest, Hungary. toldigergely@yahoo.com
authors.forename: Gergely
authors.initials: G
authors.lastname: Toldi
authors.forename: Balázs
authors.initials: B
authors.lastname: Stenczer
authors.forename: András
authors.initials: A
authors.lastname: Treszl
authors.forename: Szonja
authors.initials: S
authors.lastname: Kollár
authors.forename: Attila
authors.initials: A
authors.lastname: Molvarec
authors.forename: Tivadar
authors.initials: T
authors.lastname: Tulassay
authors.forename: János
authors.initials: J
authors.lastname: Rigó
authors.forename: Barna
authors.initials: B
authors.lastname: Vásárhelyi
mesh_terms: D000328:Adult; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051664:Kv1.3 Potassium Channel / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D011225:Pre-Eclampsia / Q000276:immunology; D011247:Pregnancy / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000502:physiology
chemical_list: D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C496923:KCNN4 protein, human; D051664:Kv1.3 Potassium Channel; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D002118:Calcium