Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide.
We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1-CpG) induced IL-12 in the spleen cells of naïve mice. Cry j 1-CpG immunization of BALB/c mice suppressed anti-Cry j 1 IgE response and enhanced anti-Cry j 1 IgG(2a) to subsequent Cry j 1 and alum adjuvant injection. CD4(+)T cells isolated from the spleens in mice immunized with Cry j 1-CpG produced higher IFN-γ levels than did CD4(+)T cells obtained from mice as negative controls. Our results suggested that Cry j 1-CpG immunization can induce Cry j 1-specific Th1 immune responses, thereby inhibiting IgE response to the pollen allergen.
Kaburaki
Y
Y
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Fujimura
T
T
Kurata
K
K
Masuda
K
K
Toda
M
M
Yasueda
H
H
Chida
K
K
Kawarai
S
S
Sakaguchi
M
M
D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D053762:Interleukin-12 Subunit p40 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
D052179:Antigens, Plant; C408982:CPG-oligonucleotide; C065290:Cry j I protein, Cryptomeria japonica; D007074:Immunoglobulin G; D053762:Interleukin-12 Subunit p40; D009838:Oligodeoxyribonucleotides; D010940:Plant Proteins; D007073:Immunoglobulin E