title: Estrogenic activity of a naringinase-treated extract of Sophora japonica cultivated in Egypt.
abstract: The naringinase-treated methanol extract of Sophora japonica L. (Fabaceae) seeds showed potent estrogen agonist activity. Through bioassay-guided isolation of the main active constituents from the naringinase-treated methanol extract of S. japonica, the aglycones genistein and kaempferol were found to be the main phytoestrogens in the naringinase-treated extract. In addition, kaempferol was nearly equipotent to genistein as an estrogen agonist. Concerning the compounds isolated from the untreated methanol extract, sophoricoside showed weak estrogenic activity on ERbeta only.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Ali M
authors.initials: AM
authors.lastname: El-Halawany
authors.forename: Mi Hwa
authors.initials: MH
authors.lastname: Chung
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Abdallah
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nishihara
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
mesh_terms: D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004063:Digestion; D055808:Drug Discovery; D004534:Egypt; D004965:Estrogen Antagonists / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000235:genetics; D047629:Estrogen Receptor beta / Q000819:agonists / Q000235:genetics; D019833:Genistein / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000378:metabolism*; D009994:Osmolar Concentration; D048789:Phytoestrogens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D029909:Sophora / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001578:Benzopyrans; D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D044949:Kaempferols; D009097:Multienzyme Complexes; D048789:Phytoestrogens; D010936:Plant Extracts; C120642:sophoricoside; C006552:kaempferol; D019833:Genistein; C039827:naringinase; D001617:beta-Glucosidase