Determination of trace deoxyribonucleic acid by using fluorescein isothiocyanate-phenosafranine as a double-luminescent phosphorescence probe.
Using Pb(2+) as ion perturber, phenosafranine (PF) and fluorescein isothiocyanate (FITC) could emit strong and stable room temperature phosphorescence (RTP) signal on the filter paper, respectively. When they were mixed, the phenomenon that the RTP signal of PF and FITC enhanced significantly was found. And 1.12 ag DNA spot(-1) (sample volume was 0.40 μL, corresponding concentration was 2.8 × 10(-15) g mL(-1)) could cause the RTP signal of both PF and FITC to enhance sharply. The content of DNA was proportional to the ΔI(p) of PF and FITC in the system at 634 and 659 nm. Thus, a new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace DNA was established by using FITC-PF as double-luminescent phosphorescence probe. The detection limit (LD) of this method calculated by 3S(b)/k was 14 zg DNA spot(-1) for PF and 18 zg DNA spot(-1) for FITC, respectively, showing high sensitivity. It has been applied to the determination of trace DNA in practical samples and the analysis results were in accordance with those of fluorescence probe. The reaction mechanism of SSRTP for the determination of trace DNA was also discussed.
Liu
Jia-Ming
JM
Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, People's Republic of China. zzsyliujiaming@163.com
Huang
Xiao-Mei
XM
Liu
Zhen-Bo
ZB
Li
Fei-Ming
FM
Lin
Li-Ping
LP
Wang
Xin-Xing
XX
Lin
Chang-Qing
CQ
Huang
Ya-Hong
YH
Li
Zhi-Ming
ZM
Lin
Shao-Qin
SQ
D004247:DNA / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D015335:Molecular Probes; D010619:Phenazines / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
D015335:Molecular Probes; D010619:Phenazines; C011977:phenosafranine; D004247:DNA; D016650:Fluorescein-5-isothiocyanate