Intracellular imaging of HeLa cells by non-functionalized NaYF4 : Er3+, Yb3+ upconverting nanoparticles.
We report on the efficient incorporation of non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles inside HeLa live cancer cells by direct endocytosis. The efficient two-photon excited near-infrared-to-visible upconversion fluorescence of these nanoparticles is then used to obtain high-contrast intracellular fluorescence images of single cells. These images reveal a redistribution of the nanoparticles inside the cell as the incubation time increases. Thus, non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles emerge as very promising fluorescence probes for real-time imaging of cellular dynamics.
Vetrone
Fiorenzo
F
Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada.
Naccache
Rafik
R
Juarranz de la Fuente
Angeles
A
Sanz-Rodríguez
Francisco
F
Blazquez-Castro
Alfonso
A
Rodriguez
Emma Martin
EM
Jaque
Daniel
D
Solé
José García
JG
Capobianco
John A
JA
D004871:Erbium / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
D015019:Yttrium; D004871:Erbium; D015018:Ytterbium