Characterization of seeds of selected wild species of rice (Oryza) stored under high temperature and humidity conditions.
Wild progenitors of rice (Oryza) are an invaluable resource for restoring genetic diversity and incorporating useful traits back into cultivars. Studies were conducted to characterize the biochemical changes, including SDS-PAGE banding pattern of storage proteins in seeds of six wild species (Oryza alta, O. grandiglumis, O. meridionalis, O. nivara, O. officinalis and O. rhizomatis) of rice stored under high temperature (45 degrees C) and humidity (approixmately 100%) for 15 days, which facilitated accelerated deterioration. Under the treated conditions, seeds of different wild rice species showed decrease in per cent germination and concentrations of protein and starch, but increase in conductivity of leachate and content of sugar. The SDS-PAGE analysis of seed proteins showed that not only the total number of bands, but also their intensity in terms of thickness differed for each species under storage. The total number of bands ranged from 11 to 22, but none of the species showed all the bands. Similarity index for protein bands between the control and treated seeds was observed to be least in O. rhizomatis and O. alta, while the indices were 0.7 and 0.625 for O. officinalis and O. nivara, respectively. This study clearly showed that seed deterioration led to distinctive biochemical changes, including the presence or absence as well as altered levels of intensity of proteins. Hence, SDS-PAGE protein banding pattern can be used effectively to characterize deterioration of seeds of different wild species of rice.
Das
Smruti
S
Division of Biochemistry, Plant Physiology and Environmental Sciences, Central Rice Research Institute, Cuttack 753006.
Nayak
Monalisa
M
Patra
B C
BC
Ramakrishnan
B
B
Krishnan
P
P
D000383:Agriculture; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D018525:Germination; D006358:Hot Temperature; D006813:Humidity; D012275:Oryza / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D014674:Plant Proteins, Dietary / Q000032:analysis; D012639:Seeds / Q000737:chemistry / Q000254:growth & development; D013045:Species Specificity; D013213:Starch / Q000032:analysis
D002241:Carbohydrates; D014674:Plant Proteins, Dietary; D013213:Starch