Rapid detection of trace amounts of surfactants using nanoparticles in fluorometric assays.
Rapid microtiter assays that utilize the time-resolved fluorescence resonance energy transfer or quenching of dye-labeled proteins adsorbed onto the surfaces of polystyrene or maghemite nanoparticles have been developed for the detection and quantification of trace amounts of surfactants at concentrations down to 10 nM.
Härmä
Harri
H
Laboratory of Biophysics and Medicity, University of Turku, Tykistökatu 6A, Turku, Finland. harri.harma@utu.fi
Laakso
Susana
S
Pihlasalo
Sari
S
Hänninen
Pekka
P
Faure
Bertrand
B
Rana
Subhasis
S
Bergström
Lennart
L
D004396:Coloring Agents / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005470:Fluorometry / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013501:Surface-Active Agents / Q000032:analysis*
D004396:Coloring Agents; D005290:Ferric Compounds; D011137:Polystyrenes; D011506:Proteins; D013501:Surface-Active Agents; C000499:ferric oxide