title: [2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins].
abstract: Oligonucleotides with 1,2-diol grouping were prepared from 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxo-ethyl]uridine 3'-phosphoramidite. The thermal stability of modified DNA duplexes and their ability to form complexes with the p50 subunit of the NF-kappaB transcription factor and (cytosine-5)-DNA methyltransferase SsoII were studied. The periodate oxidation of the l,2-diol grouping of the oligonucleotides resulted in reactive 2'-aldehyde derivatives. The opportunity of their use for the affinity modification of DNA-recognizing proteins was studied.
authors.forename: E A
authors.initials: EA
authors.lastname: Khomiakova
authors.forename: E V
authors.initials: EV
authors.lastname: Kazanova
authors.forename: E M
authors.initials: EM
authors.lastname: Zubin
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: N V
authors.initials: NV
authors.lastname: Molochkov
authors.forename: E M
authors.initials: EM
authors.lastname: Riazanova
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaia
mesh_terms: D000447:Aldehydes / Q000138:chemical synthesis* / Q000737:chemistry; D015257:DNA-Cytosine Methylases / Q000737:chemistry*; D008024:Ligands; D052002:NF-kappa B p50 Subunit / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000447:Aldehydes; D008024:Ligands; D052002:NF-kappa B p50 Subunit; D009841:Oligonucleotides; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases; D014529:Uridine