[Correlation between human papillomavirus type 16 infection and human leukocyte antigen class I expression in cervical cancers of Uighur women].
OBJECTIVE
To explore the relationship between human papillomavirus(HPV) infection and expression of human leukocyte antigen class I (HLA-I) family genes (HLA-A, B and C) in cervical cancers of Uighur women, and to investigate their effect on cervical cancer progression.


METHODS
Fresh tissue samples of 78 Uighur women with cervical squamous carcinoma, cervical intraepithelial neoplasia (CIN) or benign cervicitis were selected. HLA-A, B and C expression and HPV infection were analyzed using RT-PCR and HPV gene chips, respectively.


RESULTS
There was a tendency of increasing the total loss of HLA-A, B and C mRNA as the cervical lesions became more aggressive. Loss of HLA-I mRNA in CIN (I, II and III) and cervical squamous carcinoma was 70.0% (14/20) and 84.8% (39/46) respectively. Poorly differentiated cervical carcinomas had the highest HLA-I expression loss (90.6%). In contrast, HLA-I mRNA loss was seen in only 8% of cases of cervicitis. Moreover, it was found that high risk HPV 16 infection was strongly correlated with the loss HLA-I mRNA expression (r = 0.803, P < 0.01).


CONCLUSIONS
The loss of HLA-I gene expression is strongly correlated with HPV-16 infection, and may serve as a biomarker of cervical cancer progression in Uighur women.
Ma
Qi
Q
Department of Biology, Xinjiang Medical University, Urumqi 830011, China.
Hasim
Ayshamgul
A
Abudukadeer
Abida
A
Yilike
Xiamixinuer
X
Wu
Gui-zhen
GZ
Abudula
Abulizi
A
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000821:virology; D002681:China / Q000208:ethnology; D005260:Female; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism*; D015234:HLA-A Antigens / Q000235:genetics / Q000378:metabolism; D015235:HLA-B Antigens / Q000235:genetics / Q000378:metabolism; D015236:HLA-C Antigens / Q000235:genetics / Q000378:metabolism; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000821:virology; D012333:RNA, Messenger / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000821:virology; D002575:Uterine Cervicitis / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000208:ethnology / Q000235:genetics / Q000276:immunology / Q000821:virology
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D012333:RNA, Messenger