MCP-1 production in temporomandibular joint inflammation.
Synovitis, which is characterized by the infiltration of inflammatory cells, often accompanies progression of temporomandibular joint disorder (TMD) symptoms. Because IL-1β is elevated in synovial fluids obtained from TMDs, we hypothesized that IL-1β-responsive genes in synoviocytes may help identify the putative genes associated with synovitis. Using microarray analysis, we found that monocyte chemoattractant protein-1 (MCP-1) mRNA levels were elevated in IL-1β-stimulated synoviocytes. MCP-1 is a member of the chemokine superfamily. The production of MCP-1 was increased in synoviocytes treated with IL-1β. When IL-1β was injected into the cavities of rat TMJs, inflammatory cells and MCP-1-positive cells were detected in the synovial tissues. Furthermore, MCP-1 levels were higher in synovial fluids from individuals with pain compared with those without pain. Inhibitors of MAP-kinases and NF-κB reduced IL-1β-induced MCP-1 production. These results suggest that MCP-1 stimulated by IL-1β is one of the factors associated with the inflammatory progression of TMDs.
Ogura
N
N
Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan.
Satoh
K
K
Akutsu
M
M
Tobe
M
M
Kuyama
K
K
Kuboyama
N
N
Sakamaki
H
H
Kujiraoka
H
H
Kondoh
T
T
D000293:Adolescent; D000328:Adult; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D001324:Autoantigens / Q000032:analysis / Q000187:drug effects; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D018929:Cell Culture Techniques; D018932:Chemokine CCL2 / Q000032:analysis* / Q000187:drug effects; D016207:Cytokines / Q000032:analysis / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D004204:Joint Dislocations / Q000276:immunology; D008297:Male; D046228:Microarray Analysis; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010003:Osteoarthritis / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D013582:Synovial Fluid / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D013583:Synovial Membrane / Q000187:drug effects / Q000276:immunology; D013585:Synovitis / Q000276:immunology; D019224:Temporomandibular Joint Disc / Q000276:immunology; D013705:Temporomandibular Joint Disorders / Q000276:immunology*; D055815:Young Adult
D000873:Anthracenes; D001324:Autoantigens; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D016207:Cytokines; D005419:Flavonoids; D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; C432165:pyrazolanthrone; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one