Design and evaluation in mice of a broadly protective meningococcal group B native outer membrane vesicle vaccine.
A vaccine based on native outer membrane vesicles (NOMV) that has potential to provide safe, broad based protection against group B strains of Neisseria meningitidis has been developed. Three antigenically diverse group B strains of N. meningitidis were chosen and genetically modified to improve safety and expression of desirable antigens. Safety was enhanced by disabling three genes: synX, lpxL1, and lgtA. The vaccine strains were genetically configured to have three sets of antigens each with potential to induce protective antibodies against a wide range of group B strains. Preliminary immunogenicity studies with combined NOMV from the three strains confirmed the capacity of the vaccine to induce a broad based bactericidal antibody response. Analysis of the bactericidal activity indicated that antibodies to the LOS were responsible for a major portion of the bactericidal activity and that these antibodies may enhance the bactericidal activity of anti-protein antibodies.
Zollinger
Wendell D
WD
Division of Bacterial and Rickettsial Diseases, 503 Robert Grant Ave., Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD 20910, USA. wendell.zollinger@gmail.com
Donets
Mikhail A
MA
Schmiel
Deborah H
DH
Pinto
Valerian B
VB
Labrie
Joseph E
JE
Moran
Elizabeth E
EE
Brandt
Brenda L
BL
Ionin
Boris
B
Marques
Ryan
R
Wu
Max
M
Chen
Ping
P
Stoddard
Mark B
MB
Keiser
Paul B
PB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D055786:Gene Knockout Techniques; D022401:Meningococcal Vaccines / Q000276:immunology*; D051379:Mice; D038541:Neisseria meningitidis, Serogroup B / Q000235:genetics* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D022401:Meningococcal Vaccines