title: A novel chemoattractant lectin, karatoxin, from the dorsal spines of the small scorpionfish Hypodytes rubripinnis.
abstract: A chemoattractant lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, was isolated using a combination of affinity chromatography techniques. The glycoprotein, with a molecular mass of 110 kDa, is named Karatoxin. Karatoxin caused agglutination of rabbit erythrocytes. This agglutination was effectively inhibited by D-mannose. In addition, Karatoxin exhibited not only mitogenic activity in the presence of murine splenocytes, but also chemotactic activity in the presence of guinea-pig neutrophils and macrophages. Thus, Karatoxin appears to be a novel chemoattractant lectin. These results suggest that the redfin velvetfish Hypodytes rubripinnis may be a novel source of biologically active substances.
authors.affiliation: Depatment of Pharmacology, Osaka Dental University, Hirakata, Japan. shino@cc.osaka-dent.ac.jp
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Shinohara
authors.forename: Kuniko
authors.initials: K
authors.lastname: Nagasaka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Kozue
authors.initials: K
authors.lastname: Edo
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sakai
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.forename: Futoshi
authors.initials: F
authors.lastname: Iwaki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohura
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002846:Chromatography, Affinity; D005399:Fishes; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D011817:Rabbits
chemical_list: D006023:Glycoproteins; D037102:Lectins; C554065:karatoxin, Hypodytes rubripinnis