title: Measurement of anti-beta amyloid antibodies in human blood.
abstract: The human IgG repertoire contains endogenous antibodies against beta amyloid (Aβ) that may be relevant to the pathogenesis and treatment of Alzheimer's disease. There have been widely disparate estimates of the levels of these antibodies in human plasma. We identify factors that have contributed to these disparities and describe improved methods for measuring anti-Aβ antibodies in blood. These methods include isolating immunoglobulin by thiophilic chromatography and using chaotropic salts to dislodge weakly bound antibodies without significantly reducing the binding of specific anti-Aβ antibodies. Using these methods, we show that human blood contains polyvalent IgG antibodies that bind to Aβ with relatively low avidity and specificity, as well as IgG antibodies that bind to linear and conformational epitopes on amyloid monomers and aggregates with moderate to high avidity.
authors.affiliation: Department of Neurology and Neurosciences, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Szabo
authors.forename: Diana M
authors.initials: DM
authors.lastname: Mujalli
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Rotondi
authors.forename: Rajal
authors.initials: R
authors.lastname: Sharma
authors.forename: Alfred
authors.initials: A
authors.lastname: Weber
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Schwarz
authors.forename: Marc E
authors.initials: ME
authors.lastname: Weksler
authors.forename: Norman
authors.initials: N
authors.lastname: Relkin
mesh_terms: D000544:Alzheimer Disease / Q000097:blood / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000097:blood* / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood*; D011485:Protein Binding / Q000276:immunology
chemical_list: D016229:Amyloid beta-Peptides; D007074:Immunoglobulin G