Proteomic analysis of bovine omental, subcutaneous and intramuscular preadipocytes during in vitro adipogenic differentiation.
Given the substantial rise in obesity, depot-specific fat accumulation and its associated diseases like diabetes, it is important to understand the molecular basis of depot-specific adipocyte differentiation. Many studies have successfully exploited the adipocyte differentiation, but most of them were not related to depot-specificity, particularly using freshly isolated primary preadipocytes. Using 2-dimensional polyacrylamide gel electrophoresis coupled with sequencing mass spectrometry, we searched and compared the proteins differentially expressed in undifferentiated and differentiated preadipocytes from bovine omental, subcutaneous and intramuscular adipose depots. Our proteome mapping strategy to identify differentially expressed intracellular proteins during adipogenic conversion revealed 65 different proteins that were found to be common for the three depots. Further, we validated the differential expression for a subset of proteins by immunoblotting analyses. The results demonstrated that many structural proteins were down-regulated during differentiation of preadipocytes from all the depots. Most up-regulated proteins like Ubiquinol-cytochrome-c reductase complex core protein I (UQCRC1), ATP synthase D chain, Superoxide dismutase (SOD), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Sulfotransferase 1A1 (SULT1A1), Carnitine O-palmitoyltransferase 2 (CPT2) and Heat-shock protein beta 1 (HSPB1) across the three depots were found to be associated with lipid metabolism and metabolic activity. Further, all the up-regulated proteins were found to have higher protein expression in omental than subcutaneous or intramuscular depots.
Rajesh
Ramanna Valmiki
RV
Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration, Suwon, 441-706, Republic of Korea.
Heo
Gang-Nyung
GN
Park
Mi-Rim
MR
Nam
Jin-Seon
JS
Kim
Nam-Kuk
NK
Yoon
Duhak
D
Kim
Tae-Hun
TH
Lee
Hyun-Jeong
HJ
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D009132:Muscles / Q000166:cytology; D009852:Omentum / Q000166:cytology; D011506:Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation
D011506:Proteins