Treatment of phenol-contaminated soil by Corynebacterium glutamicum and toxicity removal evaluation.
Biodegradation of phenol-contaminated soils using Corynebacterium glutamicum, was optimized in this study. Phenol degradation by C. glutamicum was observed in soil supplemented with 1% yeast extract as a substrate. We determined the optimal inoculation size of C. glutamicum (7.4 log(10) CFU mL(-1)) for the degradation of phenol-contaminated soil. Under optimal conditions (1% yeast extract and 7.4 log(10) CFU mL(-1) of C. glutamicum), the efficiency of phenol degradation in soil was greater than 94% at three days after treatment. The degradation of phenol-contaminated soil by C. glutamicum was evaluated using acute toxicity assays with Daphnia magna. Based on the acute toxicity assays, we found that the residual toxicity of contaminated soil was removed completely after three days of C. glutamicum treatment.
Lee
Soo Youn
SY
Department of Bioprocess Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
Kim
Bit-Na
BN
Han
Ji-Hye
JH
Chang
Suk-Tai
ST
Choi
Young-Woo
YW
Kim
Yang-Hoon
YH
Min
Jiho
J
D002849:Chromatography, Gas; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D010636:Phenols / Q000032:analysis* / Q000633:toxicity; D012989:Soil Pollutants / Q000032:analysis* / Q000633:toxicity
D010636:Phenols; D012989:Soil Pollutants