title: Specific formation of trypsin-resistant micelles on a hydrophobic peptide observed with Triton X-100 but not with octylglucoside.
abstract: The manner of interaction of the coat peptide of the Pf3 phage (Pf3 peptide) with lipid bilayers has been extensively studied. Presently, we designed a derivative of the Pf3 peptide, referred to as the DDRK peptide, and subjected it to trypsin digestion to understand its physicochemical properties. In the presence of Triton X-100 used for solubilization of the peptide, digestion of DDRK with trypsin caused specific cleavage at the lysine (Lys) residue in its N-terminal region but not at other Lys residues or at the arginine residue. As the N-terminal region of the DDRK peptide is relatively hydrophilic, but its remaining region is hydrophobic, this hydrophobic region of the peptide would be expected to be coated by Triton micelles. Thus, we propose that the presence of such micelles protected against cleavage there, leading to selective cleavage by trypsin of the DDRK peptide at its hydrophilic Lys residue in the N-terminal part of the molecule. However, such a protective effect on the DDRK peptide against trypsin digestion was not observed with octylglucoside. The observed results are important for better understanding of the manner of interaction between detergents and hydrophobic peptides.
authors.affiliation: Institute for Genome Research, University of Tokushima, Kuramotocho-3, Tokushima 770-8503, Japan.
authors.forename: Chie
authors.initials: C
authors.lastname: Katsuda
authors.forename: Kanami
authors.initials: K
authors.lastname: Niiyama
authors.forename: Eriko
authors.initials: E
authors.lastname: Obana
authors.forename: Takenori
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Katou
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kataoka
authors.forename: Kazuto
authors.initials: K
authors.lastname: Ohkura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000595:Amino Acid Sequence; D005960:Glucosides / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D008969:Molecular Sequence Data; D017830:Octoxynol / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry*; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D008823:Micelles; D010455:Peptides; C018619:octyl-beta-D-glucoside; D017830:Octoxynol; D014357:Trypsin