The allele frequencies of HPA 1-16 determined by PCR-SSP in Chinese Cantonese donors.
BACKGROUND
Typing of human platelet antigens (HPA) has proven to be useful in some clinical situations related to platelet alloimmunization.


OBJECTIVE
The objective of this study was to investigate HPA 1-16 and to determine genotype and allele frequencies by polymerase chain reaction-sequence specific primer (PCR-SSP) in apheresis platelet donors in Guangzhou Blood Center.


METHODS
A total of 200 random samples from donors were involved in the study. Genotype and allele frequencies of HPA 1 to 16 were detected by PCR-SSP method.


RESULTS
The frequencies obtained from these donors were 99·50 and 0·50% for HPA-1a and -1b; 96·25 and 3·75% for HPA-2a and -2b; 54·25 and 45·75% for HPA-3a and -3b; 99·50 and 0·50% for HPA-4a and -4b; 99·00 and 1·00% for HPA-5a and -5b; 97·00 and 3·00% for HPA-6a and -6b and 42·25 and 57·75% for HPA-15a and -15b. There is only a/a homozygosis detected in HPA-7, -8, -9, -10, -11, -12, -13, -14 and -16. In this study, none of HPA-1b/ 1b, -2b/2b, -5b/5b homozygosis were detected which were found in other racial groups. One homozygosis of HPA-6b/6b in 200 individuals was detected which was not found in a study involving 1000 Chinese (Feng et al., 2006).


CONCLUSIONS
The HPA-3 and -15 appear to the highest priority and HPA-2, -6, -5 -1 and -4 to be the second priority in Chinese Cantonese when it comes to the diagnosis of neonatal alloimmune thrombocytopenia and to provide the HPA-matched platelet for patients with platelet transfusion refractoriness. The PCR-SSP method makes it possible to detect genotype of HPA-1 to -16 in less than 4 h and to establish a donor database for HPA genotype in a blood bank.
Nie
Y-M
YM
Institute of Clinical Blood Transfusion, Guangzhou Blood Center Division of Gastrointestinal & Pancreatic Surgery, The First Affiliated Hospital of Sun Yet-sen University, Guangzhou, China.
Zhou
H-J
HJ
Fu
Y-S
YS
Wang
C-X
CX
Ma
J-P
JP
D000328:Adult; D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001782:Blood Donors; D002681:China; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012494:Sampling Studies; D055815:Young Adult
D016824:Antigens, Human Platelet; D017931:DNA Primers