title: Reversal of age-associated oxidative stress in rats by MRN-100, a hydro-ferrate fluid.
abstract: Aging is associated with an increase in the level of reactive oxygen species (ROS), which cause cellular damage. This study examined the protective effects of MRN-100, an iron-based hydro-ferrate fluid, against age-associated oxidative stress using young (4 months old) and aged (22 months old) rats. The effect of MRN-100 intake on lipid peroxidation, protein oxidation, and antioxidant status was studied in the blood, liver, and brain. Aged rats were treated with tap water or MRN-100 (100%) orally daily for 40 days. Results revealed that in aged rats treated with MRN-100 (100%) there was: (i) an inhibition of the levels of oxidative stress biomarkers: malondialdehyde, nitric oxide, protein carbonyl groups and total free radicals; (ii) an augmentation in glutathione and total thiol contents; (iii) an enhancement in the activity of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase; and (iv) an enhancement in plasma iron and ferritin concentrations, and in transferrin saturation. MRN-100 reversed age-related oxidative changes, bringing levels to within the limits of the young control rats in the blood, liver and brain. MRN-100 showed effectiveness in modulating age-associated oxidative stress in rats, and it may therefore be useful to reduce age-associated disorders where free radicals and oxidative stress are the major cause.
authors.affiliation: Department of Zoology, Faculty of Science, University of Mansoura, Mansoura 35516, Egypt. na_ri_eg@yahoo.com
authors.forename: Nariman K
authors.initials: NK
authors.lastname: Badr El-Din
authors.forename: Eman
authors.initials: E
authors.lastname: Noaman
authors.forename: Salma M Abdel
authors.initials: SM
authors.lastname: Fattah
authors.forename: Mamdooh
authors.initials: M
authors.lastname: Ghoneum
mesh_terms: D000284:Administration, Oral; D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D017382:Reactive Oxygen Species; C017311:ferrate ion; D013972:Thyrotropin; D007501:Iron; D005978:Glutathione