Characterization and in vitro cellular uptake of PEG coated iron oxide nanoparticles as MRI contrast agent.
Monodisperse magnetic iron oxide nanoparticles (MNPs), coated with PEG at different molecular weight, were prepared via self-assembly method. The particle diameters were measured by dynamic light scattering and transmission electron microscope. Increasing the molecular weight of PEG in the coating polymer increased the overall particles diameter. As coating thickness increased, the saturation magnetization (Ms) and T2 relaxivity decreased. The interactions of these MNPs with macrophage cells were also investigated. The results showed that cellular uptakes of MNPs depended on nanoparticle concentration and surface chemistry. The results of this study will have implications on the chemical design of nanomaterials for bio-imaging and bio-detection.
Chen
Y J
YJ
Pharmaceutical Research Institute, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
Tao
J
J
Xiong
F
F
Zhu
J B
JB
Gu
N
N
Geng
K K
KK
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005290:Ferric Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D006801:Humans; D007624:KB Cells; D008264:Macrophages / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D008970:Molecular Weight; D053758:Nanoparticles; D019301:Oleic Acid / Q000737:chemistry; D010592:Pharmaceutic Aids; D011092:Polyethylene Glycols; D013778:Tetrazolium Salts; D013844:Thiazoles
D003287:Contrast Media; D005290:Ferric Compounds; D010592:Pharmaceutic Aids; D013778:Tetrazolium Salts; D013844:Thiazoles; C000499:ferric oxide; D019301:Oleic Acid; D011092:Polyethylene Glycols; C022616:thiazolyl blue