Evaluation of a simple and low cost potentiometric biosensor for pharmaceutical and in vivo adrenaline determination.
In this work the polyphenol oxidase (PPO) was the main component of a biosensor for adrenaline determination. The activity of this enzyme was measured in several vegetables. Banana (Musa sp.) extracts presented better results with 974 UA (units of activity). The biosensor was constructed with a polyethylene tube (0.8 mm i.d.) filled with: carbon paste containing 50 UA of the PPO in phosphate buffer (pH=7.00) solution and vaseline as agglutinant. When the biosensor was applied in medicine samples it provided a linear range from 8.00×10(-9) to 8.00×10(-4) mol L(-1); the results obtained with the proposed method and the Brazilian Pharmacopoeia method were in agreement (t-test). When it was applied in blood samples, the matrix-matching calibration was used, and the linear range was from 8.00×10(-7) to 8.00×10(-3) mol L(-1). In vivo studies were also done. The obtained results for those electrodes, which were inserted in the jugular vein of Wistar rats, were very promising.
Mataveli
Lidiane Raquel Verola
LR
Departamento de Ciências Exatas, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700, CEP 37130-000, Alfenas, MG, Brazil. lmataveli@iqm.unicamp.br
de Jesus Antunes
Natalícia
N
Brigagão
Maisa Ribeiro Pereira Lima
MR
de Magalhães
Cristiana Schmidt
CS
Wisniewski
Célio
C
Luccas
Pedro Orival
PO
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004837:Epinephrine / Q000097:blood*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051381:Rats
D004837:Epinephrine