Multiplex immunoassays of equine virus based on fluorescent encoded magnetic composite nanoparticles.
A new detection format for multiplexed analysis based on fluorescent encoded magnetic composite nanoparticles is presented. Two kinds of virus were analyzed by this new method: equine influenza virus (EIV) and equine infectious anemia virus (EIAV). Firstly, EIV antigen and EIAV antigen were conjugated to two kinds of fluorescent encoded magnetic composite nanoparticles, while the green-emitting CdTe quantum dots (QDs) were attached to the antibody of EIV and EIAV. Then both green-emitting CdTe QD-labeled antibodies and antigens labeled with fluorescent encoded magnetic composite nanoparticles were used to form an immunoassay system for the detection of EIV and EIAV antigens. The method is time-saving and has higher sensitivity (1.3 ng mL(-1) for EIV antigens and 1.2 ng mL(-1) for EIAV antigens) than the conventional methods. A competitive immunoassay method based on this analysis system was used to detect EIV and EIAV antigens in spiked serum samples with satisfactory results.
Wang
Guannan
G
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Gao
Yuan
Y
Huang
Hui
H
Su
Xingguang
X
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D019187:Cadmium Compounds / Q000737:chemistry; D004859:Equine Infectious Anemia / Q000175:diagnosis; D005456:Fluorescent Dyes / Q000737:chemistry; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D004860:Infectious Anemia Virus, Equine / Q000276:immunology / Q000302:isolation & purification*; D053123:Influenza A Virus, H3N8 Subtype / Q000276:immunology / Q000302:isolation & purification*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009976:Orthomyxoviridae Infections / Q000175:diagnosis; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000191:economics / Q000379:methods; D013691:Tellurium / Q000737:chemistry
D000906:Antibodies; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D013691:Tellurium; C028337:cadmium telluride