Collective chemotaxis requires contact-dependent cell polarity.
Directional collective migration is now a widely recognized mode of migration during embryogenesis and cancer. However, how a cluster of cells responds to chemoattractants is not fully understood. Neural crest cells are among the most motile cells in the embryo, and their behavior has been likened to malignant invasion. Here, we show that neural crest cells are collectively attracted toward the chemokine Sdf1. While not involved in initially polarizing cells, Sdf1 directionally stabilizes cell protrusions promoted by cell contact. At this cell contact, N-cadherin inhibits protrusion and Rac1 activity and in turn promotes protrusions and activation of Rac1 at the free edge. These results show a role for N-cadherin during contact inhibition of locomotion, and they reveal a mechanism of chemoattraction likely to function during both embryogenesis and cancer metastasis, whereby attractants such as Sdf1 amplify and stabilize contact-dependent cell polarity, resulting in directional collective migration.
Theveneau
Eric
E
Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK.
Marchant
Lorena
L
Kuriyama
Sei
S
Gull
Mazhar
M
Moepps
Barbara
B
Parsons
Maddy
M
Mayor
Roberto
R
D000818:Animals; D030801:Animals, Genetically Modified; D015820:Cadherins / Q000235:genetics / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology*; D003260:Contact Inhibition / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology; D008954:Models, Biological; D009432:Neural Crest / Q000166:cytology / Q000196:embryology; D019718:Receptors, CXCR4 / Q000235:genetics / Q000502:physiology; D014981:Xenopus / Q000196:embryology / Q000235:genetics / Q000502:physiology; D029867:Xenopus Proteins / Q000235:genetics / Q000502:physiology
C449185:CXCL12 protein, Xenopus; D015820:Cadherins; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D029867:Xenopus Proteins