The reaction mechanism of allene oxide synthase: Interplay of theoretical QM/MM calculations and experimental investigations.
A combined theoretical and experimental study highlights the reaction mechanism of allene oxide synthase (AOS) and its possible link to hydroperoxide lyase (HPL) pathway. A previously published study (Lee et al., Nature 455 (2008) 363) has shown that the F137 residue is of central importance in differentiating between the AOS and HPL pathways after initial identical steps. In the experimental part of this study, we show that wild-type AOS from Arabidopsis or rice in fact produces both AOS and HPL products in a ratio of about 80:15, something that was found only in trace amounts before. Theoretical calculations successfully map the whole AOS pathway with 13(S)-hydroperoxy linolenic and linoleic acid as substrates. Subsequent calculations investigated the effects of in silico F137L mutation at the suggested diverging point of the two pathways. The results show that QM/MM calculations can reasonably reproduce three out of four experimentally available cases, and confirm that the pathways are energetically very close to each other, thus making a switch from one path to other plausible under different circumstances.
Cho
Kyung-Bin
KB
Institute of Chemistry and The Lise Meitner-Minerva Center for Quantum Computational Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.
Lai
Wenzhen
W
Hamberg
Mats
M
Raman
C S
CS
Shaik
Sason
S
D000446:Aldehyde-Lyases / Q000378:metabolism; D000466:Alkadienes / Q000378:metabolism; D017360:Arabidopsis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008041:Linoleic Acids / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D012275:Oryza / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D010087:Oxides / Q000378:metabolism; D011789:Quantum Theory
D000466:Alkadienes; D004852:Epoxy Compounds; D008041:Linoleic Acids; D010087:Oxides; C025947:propadiene; D003577:Cytochrome P-450 Enzyme System; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase