Determination of mitoxantrone in rat plasma by liquid chromatography-tandem mass spectrometry method: Application to a pharmacokinetic study.
A rapid, sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method has been developed for quantification of mitoxantrone in rat plasma. The analyte and palmatine (internal standard) were extracted from plasma samples with diethyl ether-dichloromethane (3:2, v/v) and separated on a C(18) column. The chromatographic separation was achieved within 2.5min using methanol-10mM ammonium acetate containing 0.1% acetic acid as the mobile phase at a flow rate of 0.2mL/min. The method was linear over the range of 0.5-500ng/mL. The lower limit of quantification (LLOQ) was 0.5ng/mL. Finally, the method was successfully applied to a pharmacokinetic study of mitoxantrone in rats following intravenous administration.
Zhang
Peng
P
School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
Ling
Guixia
G
Sun
Jin
J
Sun
Yongbing
Y
Pu
Xiaohui
X
Wang
Zhiyuan
Z
He
Zhonggui
Z
D000704:Analysis of Variance; D000818:Animals; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D008942:Mitoxantrone / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001600:Berberine Alkaloids; D008942:Mitoxantrone; C005413:palmatine