Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells.
Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5-week-old Wistar rats, were placed in alpha-modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and beta-glycerophosphate (beta-GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days. Conventional and real-time Polymerase Chain Reaction (PCR) was performed to determine the expression of Notch-related genes and dentin sialophosphoprotein as a marker of odontoblast differentiation. Dentin sialophosphoprotein and Hey1 expression was significantly increased and decreased in the presence of AA + beta-GP compared with controls, respectively. These findings suggest that Hey1 may be a negative regulator in odontoblast differentiation.
Sun
Hantang
H
Department of Operative Dentistry and Endodontics, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Kawashima
Nobuyuki
N
Xu
Jing
J
Takahashi
Satomi
S
Suda
Hideaki
H
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D051792:Basic Helix-Loop-Helix Proteins / Q000032:analysis* / Q000235:genetics; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D024042:Collagen Type I; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D003907:Dexamethasone / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005938:Glucocorticoids / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D018257:Helix-Loop-Helix Motifs / Q000235:genetics*; D018398:Homeodomain Proteins / Q000032:analysis; D008297:Male; D009804:Odontoblasts / Q000502:physiology; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D051881:Receptor, Notch1 / Q000032:analysis; D051883:Receptor, Notch2 / Q000032:analysis; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000032:analysis / Q000235:genetics*; D012097:Repressor Proteins / Q000032:analysis* / Q000235:genetics; D012795:Sialoglycoproteins / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics*; D000072056:Transcription Factor HES-1; D000099311:Dentin Sialophosphoprotein
D001205:Ascorbic Acid; D051792:Basic Helix-Loop-Helix Proteins; D024042:Collagen Type I; D003470:Culture Media; D003907:Dexamethasone; D016326:Extracellular Matrix Proteins; D005938:Glucocorticoids; D005994:Glycerophosphates; D018398:Homeodomain Proteins; D010750:Phosphoproteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000071656:Receptor, Notch3; D051880:Receptors, Notch; D012097:Repressor Proteins; D012795:Sialoglycoproteins; D000072056:Transcription Factor HES-1; D000099311:Dentin Sialophosphoprotein; C405694:Hairy, HRT1 protein; C489735:Hes1 protein, rat; C489686:Hes5 protein, rat; C497763:Notch1 protein, rat; C497766:Notch2 protein, rat; C498556:Notch3 protein, rat; C031463:beta-glycerophosphoric acid