Proteome profile of the developing maize (Zea mays L.) rachis.
In this study, we performed the first high-throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2-DE and 2-D LC, we identified 967 proteins. A 2-D proteome reference map was established. Functional classification of identified proteins revealed that proteins involved in various cellular metabolisms, response to stimulus and transport, were the most abundant.
Pechanova
Olga
O
Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS 39762, USA.
Pechan
Tibor
T
Ozkan
Seval
S
McCarthy
Fiona M
FM
Williams
W Paul
WP
Luthe
Dawn S
DS
D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry*
D010446:Peptide Fragments; D010940:Plant Proteins; D020543:Proteome; D014357:Trypsin