Quantitative analysis of six polyynes and one polyene in Oplopanax horridus and Oplopanax elatus by pressurized liquid extraction and on-line SPE-HPLC.
A pressurized liquid extraction and on-line SPE-HPLC method was developed for simultaneous determination of six polyynes, including falcarindiol, oplopandiol, (11S,16S,9Z)-9,17-octadecadiene-12,14-diyne-1,11,16-triol,1-acetate, oplopandiol acetate, oplopantriol A, oplopantriol B, and one polyene, (S,E)-nerolidol, in Oplopanax horridus and Oplpanax elatus. The analysis was conducted on a Grace Prevail C(18) column (3 microm, 7 mm x 33 mm) with gradient elution of acetonitrile and water after the sample loaded and washed with 42%ACN in 0.3 min on a phenomenex Strata-X on-line Extraction Cartridge SPE column (2.5 microm, 2.0 x 20 mm). All calibration curves of seven analytes showed good linearity within the test ranges. The validated method was successfully applied to quantify six polyynes and one polyene in two species of Oplopanax, O. horridus and O. elatus.
Huang
Weihua
W
Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
Yang
Jing
J
Zhao
Jing
J
Wang
Chong-zhi
CZ
Yuan
Chun-su
CS
Li
Shao-ping
SP
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028002:Oplopanax / Q000737:chemistry*; D011090:Polyenes / Q000032:analysis*; D053279:Polyynes / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*
D011090:Polyenes; D053279:Polyynes