title: Plant-based food and feed protein structure changes induced by gene-transformation, heating and bio-ethanol processing: a synchrotron-based molecular structure and nutrition research program.
abstract: Unlike traditional "wet" analytical methods which during processing for analysis often result in destruction or alteration of the intrinsic protein structures, advanced synchrotron radiation-based Fourier transform infrared microspectroscopy has been developed as a rapid and nondestructive and bioanalytical technique. This cutting-edge synchrotron-based bioanalytical technology, taking advantages of synchrotron light brightness (million times brighter than sun), is capable of exploring the molecular chemistry or structure of a biological tissue without destruction inherent structures at ultra-spatial resolutions. In this article, a novel approach is introduced to show the potential of the advanced synchrotron-based analytical technology, which can be used to study plant-based food or feed protein molecular structure in relation to nutrient utilization and availability. Recent progress was reported on using synchrotron-based bioanalytical technique synchrotron radiation-based Fourier transform infrared microspectroscopy and diffused reflectance infrared Fourier transform spectroscopy to detect the effects of gene-transformation (Application 1), autoclaving (Application 2), and bio-ethanol processing (Application 3) on plant-based food and feed protein structure changes on a molecular basis. The synchrotron-based technology provides a new approach for plant-based protein structure research at ultra-spatial resolutions at cellular and molecular levels.
authors.affiliation: College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, Canada. peiqiang.yu@usask.ca
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Yu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D016000:Cluster Analysis; D004044:Dietary Proteins / Q000032:analysis*; D015394:Molecular Structure; D015999:Multivariate Analysis; D052756:Nutritional Sciences; D010940:Plant Proteins / Q000737:chemistry*; D018514:Plant Structures; D010945:Plants, Edible / Q000737:chemistry*; D025341:Principal Component Analysis; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D017356:Synchrotrons; D014170:Transformation, Genetic
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins