Human myeloid dendritic cells for cancer therapy: does maturation matter?
Dendritic cells form the connection between innate and adoptive mechanisms of the immune system. As antigen-presenting cells, dendritic cells are capable of presenting tumour antigen and effectively stimulating immune response targeted against a tumour. A number of preclinical and clinical studies document dendritic cells' potential in anti-cancer treatment. Increasing knowledge of dendritic cell biology is leading to improved methods for their preparation for clinical application. Unfortunately, there is to date no consensus specifying optimal conditions for dendritic cell preparation in vitro. This review summarizes the methods used for preparing myeloid dendritic cells derived from monocytic precursors while focusing on cytokine cocktails used for their growth, maturation, and functional adjustment.
Skalova
Katerina
K
University Cell Immunotherapy Center, Babak Research Institute, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. katka.skalova@gmail.com
Mollova
Klara
K
Michalek
Jaroslav
J
D000951:Antigens, Neoplasm / Q000276:immunology; D018929:Cell Culture Techniques; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology
D000951:Antigens, Neoplasm; D016207:Cytokines