The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance.
Farnesol (FOH) is a nonsterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. These isoprenoids inhibit proliferation and induce apoptosis. Here, we show that Aspergillus nidulans AifA encoding the apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase plays a role in the function of the mitochondrial Complex I. Additionally, we demonstrated that ndeA-B and ndiA encode external and internal alternative NADH dehydrogenases, respectively, that have a function in FOH resistance. When exposed to FOH, the ΔaifA and ΔndeA strains have increased ROS production while ΔndeB, ΔndeA ΔndeB, and ΔndiA mutant strains showed the same ROS accumulation than in the absence of FOH. We observed several compensatory mechanisms affecting the differential survival of these mutants to FOH.
Dinamarco
Taísa Magnani
TM
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café S/N, São Paulo, Brazil.
Pimentel
Bárbara de Castro Figueiredo
Bde C
Savoldi
Marcela
M
Malavazi
Iran
I
Soriani
Frederico Marianetti
FM
Uyemura
Sérgio Akira
SA
Ludovico
Paula
P
Goldman
Maria Helena S
MH
Goldman
Gustavo Henrique
GH
D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D005204:Farnesol / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
D051033:Apoptosis Inducing Factor; D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D005204:Farnesol; D042967:Electron Transport Complex I