High-throughput in vivo vertebrate screening.
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Pardo-Martin
Carlos
C
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.
Chang
Tsung-Yao
TY
Koo
Bryan Kyo
BK
Gilleland
Cody L
CL
Wasserman
Steven C
SC
Yanik
Mehmet Fatih
MF
D000818:Animals; D001331:Automation; D001369:Axons; D004625:Embryo, Nonmammalian; D004867:Equipment Design; D053685:Laser Therapy; D008403:Mass Screening / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D023421:Models, Animal; D009154:Mutation; D012160:Retina / Q000166:cytology*; D015027:Zebrafish