title: Temporal and spatial expression of TGF-beta1 in an Achilles tendon section model after application of platelet-rich plasma.
abstract: BACKGROUND
  To investigate the effect of platelet-rich plasma (PRP) on TGF-beta1 expression during tendon healing.
  
  
  METHODS
  We used 48 skeletally mature New Zealand White rabbits. 24 rabbits received the PRP, and 24 rabbits served as an untreated control group. Equal numbers of animals were sacrificed at 1st, 2nd, 3rd, and 4th week. The surgical procedure involved a transverse incision to transect the Achilles tendon. A volume of 1ml of PRP was then injected into the tendon mass in the PRP group. Histological and immunohistochemical evaluations with an anti-TGF-beta primary antibody were performed.
  
  
  RESULTS
  The pattern of expression of TGF-beta1 in the PRP group was characterized by a significant upregulation during the first 2 weeks and subsequently significant downregulation in the 3rd and 4th week in comparison with the controls.
  
  
  CONCLUSIONS
  Our results suggest that PRP may affect the tendon healing process by altering the expression of TGF-beta1.
authors.affiliation: Democritus University of Thrace, Department of Orthopaedic and Trauma Surgery, Dragana, BO 84100, Greece.
authors.forename: Dimitrios N
authors.initials: DN
authors.lastname: Lyras
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Kazakos
authors.forename: Marios
authors.initials: M
authors.lastname: Tryfonidis
authors.forename: George
authors.initials: G
authors.lastname: Agrogiannis
authors.forename: Sotirios
authors.initials: S
authors.lastname: Botaitis
authors.forename: Anna
authors.initials: A
authors.lastname: Kokka
authors.forename: George
authors.initials: G
authors.lastname: Drosos
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Tilkeridis
authors.forename: Dionysios
authors.initials: D
authors.lastname: Verettas
mesh_terms: D000125:Achilles Tendon / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D016512:Ankle Injuries / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012421:Rupture; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D053773:Transforming Growth Factor beta1