Pattern formation and molecular transport of histidine-tagged GFPs using supported lipid bilayers.
We fabricated a heterogeneous supported lipid bilayer (SLB) by employing binary lipid mixtures comprising a saturated acyl chain DSPC and an unsaturated acyl chain nickel-chelating lipid. By using the specific adsorption properties of histidine-tagged proteins (His-tagged GFPs) in relation to nickel-chelating lipids, we demonstrated protein pattern formation on the SLB corresponding to the phase separation pattern of the SLB. In addition, by using a lipid mixture consisting of an unsaturated acyl chain DOPC and a nickel-chelating lipid, and His-tagged GFPs, we succeeded in transporting the proteins along a hydrophilic micropattern on a SiO(2) substrate. The protein transport is induced by the self-spreading behavior of a fluid SLB with a kinetic spreading coefficient beta = 10.4 microm(2) s(-1). This method provides a guide for strategically carrying various biomolecules to specific positions by using a soft biointerface on a solid surface. In addition, the results demonstrate the importance of using techniques that allow the controlled manipulation of biomolecules based on the static or dynamic properties of the SLB platform.
Nakashima
Hiroshi
H
Materials Science Research Laboratory, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan. nakasima@nttbrl.jp
Furukawa
Kazuaki
K
Kashimura
Yoshiaki
Y
Sumitomo
Koji
K
Shinozaki
Youichi
Y
Torimitsu
Keiichi
K
D002614:Chelating Agents / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D021381:Protein Transport
D002614:Chelating Agents; D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines; D049452:Green Fluorescent Proteins; D006639:Histidine; D009532:Nickel