Effects of pinealectomy and melatonin supplementation on endometrial explants in a rat model.
OBJECTIVE
To determine the effects of pinealectomy on endometrial explants in rats and evaluate the activity of superoxide dismutase (SOD) and catalase (CAT) and the levels of malondialdehyde (MDA) in the rat endometriosis model.


METHODS
Rats with experimentally induced endometriosis were randomly divided into three groups after second-look laparotomies. Group 1 (pinealectomy, n = 8) and Group 2 (pinealectomy+melatonin, n = 8) underwent pinealectomies after the second-look laparotomies. Group 3 was presented as control group (vehicle solution+without pinealectomy (n = 6)). Melatonin was administered intraperitoneally for 4 weeks in Group 2, whereas an equal volume of vehicle solution was given to Groups 1 and 3. Evaluation of the volume of the endometrial explants, histopathological examination and preservation of explant epitheliums according to the scoring system were undertaken.


RESULTS
There was a statistically significant increase in spherical explant volumes of Group 1 compared to Groups 2 and 3. In Group 1, the level of MDA was significantly higher and SOD and CAT activity was significantly lower compared to Groups 2 and 3. A statistically significant increase in the epithelial lining scores of explants was noted in Group 1 compared to Groups 2 and 3.


CONCLUSIONS
The effects of pinealectomy on the progression of endometriosis explants were reversed by melatonin.
Koc
Onder
O
Department of Obstetrics and Gynecology, Faculty of Medicine, Abant Izzet Baysal University, Gölköy, Bolu, Turkey. onderkoc1@hotmail.com
Gunduz
Bülent
B
Topcuoglu
Ata
A
Bugdayci
Güler
G
Yilmaz
Fahri
F
Duran
Bülent
B
D000818:Animals; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004715:Endometriosis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism; D004717:Endometrium / Q000637:transplantation*; D005260:Female; D008315:Malondialdehyde / Q000032:analysis; D008550:Melatonin / Q000008:administration & dosage*; D018384:Oxidative Stress; D010870:Pineal Gland / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar
D008315:Malondialdehyde; D008550:Melatonin