title: Complete genomic sequence of a border disease virus isolated from Pyrenean chamois.
abstract: This report describes the full-length genome sequence of the pestivirus strain H2121 which was recently isolated from Pyrenean chamois and typed as Border disease virus (BDV) genotype 4. Comparison with full-length genomic sequences of the approved pestivirus species Bovine viral diarrhea virus-1 (BVDV-1), BVDV-2, BDV, and Classical swine fever virus, the tentative species represented by strain Giraffe-1, as well as the atypical pestivirus strain Th/04_KhonKaen confirmed that the chamois pestivirus strain is most similar to BDV. The viral genome of H2121 is 12,305 nucleotides long and contains one large open reading frame. The latter encodes a polyprotein consisting of 3899 amino acids and is flanked with 376 nucleotides long 5' untranslated region (UTR) and 229 nt long 3' UTR. The genome organization of the chamois virus is reminiscent to that of other pestiviruses. Compared to other BDV strains including BDV-1 strain X818 and BDV-2 strain Reindeer-1, the 5' UTR and ORF of the chamois virus are very similar in length, while the 3' UTR of H2121 is 31-44 nucleotides shorter. In contrast to other BDV strains, the genome of the chamois virus contains a unique four amino acid insertion at the N-terminus of NS2.
authors.affiliation: University of Veterinary Medicine and Pharmacy, Dept. of Epizootiology and Parasitology, Komensky 73, Kosice, Slovakia. vilcek@uvm.sk
authors.forename: Stefan
authors.initials: S
authors.lastname: Vilcek
authors.forename: Kim
authors.initials: K
authors.lastname: Willoughby
authors.forename: Peter
authors.initials: P
authors.lastname: Nettleton
authors.forename: Paul
authors.initials: P
authors.lastname: Becher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001882:Border Disease / Q000821:virology*; D018083:Border disease virus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D048608:Rupicapra; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins