title: Fibroblast growth factor-2 stimulates the proliferation of mesenchyme-derived progenitor cells from aging mouse and human bone.
abstract: The potential of fibroblast growth factor-2 (FGF-2) to stimulate osteoprogenitors in aging bone was investigated. Previous work showed a decrease in bone formation in cell cultures derived from bone of elderly female patients, but not in cells from age-matched male or younger female patients, with transforming growth factor β increasing bone formation but not increasing osteoprogenitors. In the present study, FGF-2 was shown to significantly stimulate, in a dose-dependent manner, proliferation of mesenchyme-derived progenitor cells from bones of young and old mouse and humans. In proliferation assays, human cells were more responsive to lower concentrations (0.0016 ng/mL) of FGF-2 than mouse cells, but proliferation was less in cells from older bone. Immunofluorescence microscopy revealed that FGF-2 increased and prevented the decline in cells expressing activated leukocyte cell adhesion molecule, a novel marker for early lineage osteoblasts, but not α-smooth muscle actin. FGF-2 may have therapeutic potential for stimulating osteoblast progenitors in aging.
authors.affiliation: Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington 06030-3105, USA.
authors.forename: Guomin
authors.initials: G
authors.lastname: Ou
authors.forename: Lyndon
authors.initials: L
authors.lastname: Charles
authors.forename: Seth
authors.initials: S
authors.lastname: Matton
authors.forename: Craig
authors.initials: C
authors.lastname: Rodner
authors.forename: Marja
authors.initials: M
authors.lastname: Hurley
authors.forename: Liisa
authors.initials: L
authors.lastname: Kuhn
authors.forename: Gloria
authors.initials: G
authors.lastname: Gronowicz
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D055815:Young Adult
chemical_list: D016222:Fibroblast Growth Factor 2