Modification of the chiral selectivity of D-glucose oxidase and L-lactate oxidase in a collagen matrix.
A chiral, biocompatible, conducting material comprising collagen I, silica gel and ferrocene was found to modify and invert, the chiral selectivity of D-glucose oxidase and L-lactate oxidase. This result may have implications as collagen matrices are widely used in drug delivery, biosensing and tissue engineering. Investigations were carried out using electrochemical methods, FTIR spectroscopy and circular dichroism.
Kataky
Ritu
R
Department of Chemistry, Durham University, South Road, Durham, UK, DH1 3LE.
Zawawi
Ruzniza Mohamed
RM
D015374:Biosensing Techniques; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D000075163:Metallocenes; D006899:Mixed Function Oxygenases / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013237:Stereoisomerism
D005296:Ferrous Compounds; D000075163:Metallocenes; D003094:Collagen; D006899:Mixed Function Oxygenases; D005949:Glucose Oxidase; C021497:lactate 2-monooxygenase; C004998:ferrocene