Effects of 7-epiclusianone on Streptococcus mutans and caries development in rats.
The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on surface-adsorbed glucosyltransferase (Gtf) B activity, acid production, and viability of S. MUTANS in biofilms, as well as on caries development using a rodent model. 7-Epi (100 µg/mL) significantly reduced the activity of surface-adsorbed GtfB (up to 48.0 ± 1.8 of inhibition at 100 µg/mL) and glycolytic pH-drop by S. mutans in biofilms (125 and 250 µg/mL) (vs. vehicle control, p < 0.05). In contrast, the test compound did not significantly affect the bacterial viability when compared to vehicle control (15 % ethanol, p > 0.05). Wistar rats treated topically with 7-epi (twice daily, 60-s exposure) showed significantly smaller number of and less severe smooth- and sulcal-surface carious lesions (p < 0.05), without reducing the S. mutans viable population from the animals' dental biofilms. In conclusion, the natural compound 7-epiclusianone may be a potentially novel pharmacological agent to prevent and control dental caries disease.
Branco-de-Almeida
Luciana Salles
LS
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas, SP, Brazil.
Murata
Ramiro Mendonça
RM
Franco
Eliane Melo
EM
dos Santos
Marcelo Henrique
MH
de Alencar
Severino Matias
SM
Koo
Hyun
H
Rosalen
Pedro Luiz
PL
D000818:Animals; D001577:Benzophenones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016227:Benzoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D028084:Clusiaceae / Q000737:chemistry; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051381:Rats; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
C118110:7-epiclusianone; D001577:Benzophenones; D016227:Benzoquinones; D005936:Glucans; D005964:Glucosyltransferases