A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens.
A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.
Wang
Ya-Fen
YF
Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chungli, 320, Taiwan.
Chao
How-Ran
HR
Wu
Chia-Hsin
CH
Tseng
Chu-Han
CH
Kuo
Ya-Ting
YT
Tsou
Tsui-Chun
TC
D050156:Adipogenesis; D001681:Biological Assay / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000032:analysis*; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis*; D020025:Peroxisome Proliferators / Q000378:metabolism*; D020218:Response Elements
D004785:Environmental Pollutants; D020025:Peroxisome Proliferators; D008156:Luciferases