New culture technique of human eliminable feeder-assisted target cell sheet production.
Cultured cell sheets for transplantation generally have been co-cultured with animal feeder cells, which carry risks because of different species and results in non-contact culture between the feeder and target cells. We developed a new technique to produce human eliminable feeder-assisted target cell sheets by novel human-derived genetically modified feeder cells. Three genes (human-derived telomerase reverse transcriptase gene, enhanced green fluorescent protein gene, and herpes simplex virus thymidine kinase gene) were transducted into human stromal cells, which enabled genetically modified feeder cells to be immortalized, labeled, and eliminated as needed. A target cell sheet was produced as one sheet by assisting the genetically modified feeder cells and successfully transplanted in vivo without their contamination. Genetically modified human eliminable feeder cells could be a promising tool for cultivated cell sheet transplantation.
Takamatsu
Fumihiko
F
Department of Ophthalmology, Osaka University Medical School, Suita, Japan.
Inoue
Tomoyuki
T
Li
Yingli
Y
Hori
Yuichi
Y
Maeda
Naoyuki
N
Tano
Yasuo
Y
Nishida
Kohji
K
D000818:Animals; D018929:Cell Culture Techniques; D017690:Cell Transplantation; D018920:Coculture Techniques; D019573:Epithelium, Corneal / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D018139:Simplexvirus / Q000201:enzymology; D017154:Stromal Cells / Q000378:metabolism; D019098:Telomerase / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics; D014161:Transduction, Genetic
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D013937:Thymidine Kinase; D019098:Telomerase