title: Glycodelin A suppresses the cytolytic activity of CD8+ T lymphocytes.
abstract: Maternal tolerance to the semi-allogenic fetus is brought about by several mechanisms in humans. Glycodelin A (GdA), secreted by the uterine mucosa and decidua is induced to high levels by progesterone between 12 and 16 weeks of pregnancy. The glycoprotein, an immunomodulator has been shown to be inhibitory to the survival and functions of almost all the immune cells. CD8(+) T cells which predominate the T lymphocyte population in the decidua are relatively less studied. We attempted to find out the possible mechanism, if any, of regulation of the cytolytic function of CD8(+) T cells during pregnancy. Alloactivated CD8(+) T cells harbouring specific cytolytic activity against target cells exhibited compromised activity upon treatment with high concentrations of GdA. Interestingly, unlike the CD4(+) T cells, CD8(+) T cells were resistant to GdA-induced apoptosis. The inhibition of cytotoxic T lymphocyte activity was brought about by the downregulation of transcription of the cytolytic effector molecules, granzyme B and perforin and the degranulation of cytolytic vesicles. These results suggest a protective role played by GdA during pregnancy by regulating the cytolytic activity of CD8(+) T cells.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India.
authors.forename: Chetna
authors.initials: C
authors.lastname: Soni
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Karande
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D017209:Apoptosis; D045744:Cell Line, Tumor / Q000528:radiation effects; D018920:Coculture Techniques; D003594:Cytoplasmic Granules / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D003656:Decidua / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000276:immunology; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000276:immunology; D000071816:Glycodelin; D006023:Glycoproteins / Q000502:physiology*; D053804:Granzymes / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology; D008213:Lymphocyte Activation; D010051:Ovarian Neoplasms / Q000473:pathology; D054353:Perforin / Q000096:biosynthesis / Q000235:genetics; D011247:Pregnancy / Q000276:immunology*; D011257:Pregnancy Proteins / Q000502:physiology*; D011374:Progesterone / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014599:Uterus / Q000378:metabolism
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D054353:Perforin; D011374:Progesterone; D053804:Granzymes