Ciprofloxacin-modified electrosynthesized hydrogel coatings to prevent titanium-implant-associated infections.
New promising and versatile materials for the development of in situ sustained release systems consisting of thin films of either poly(2-hydroxyethyl methacrylate) or a copolymer based on poly(ethylene-glycol diacrylate) and acrylic acid were investigated. These polymers were electrosynthesized directly on titanium substrates and loaded with ciprofloxacin (CIP) either during or after the synthesis step. X-ray photoelectron spectroscopy was used to check the CIP entrapment efficiency as well as its surface availability in the hydrogel films, while high-performance liquid chromatography was employed to assess the release property of the films and to quantify the amount of CIP released by the coatings. These systems were then tested to evaluate the in vitro inhibition of methicillin-resistant Staphylococcus aureus (MRSA) growth. Moreover, a model equation is proposed which can easily correlate the diameter of the inhibition haloes with the amount of antibiotic released. Finally, MG63 human osteoblast-like cells were employed to assess the biocompatibility of CIP-modified hydrogel coatings.
De Giglio
E
E
Department of Chemistry, University of Bari, Aldo Moro, Via E. Orabona 4, I-70126 Bari, Italy. e.degiglio@chimica.uniba.it
Cometa
S
S
Ricci
M A
MA
Cafagna
D
D
Savino
A M
AM
Sabbatini
L
L
Orciani
M
M
Ceci
E
E
Novello
L
L
Tantillo
G M
GM
Mattioli-Belmonte
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis*; D007700:Kinetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control*; D014025:Titanium / Q000009:adverse effects*
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002939:Ciprofloxacin; D014025:Titanium