title: A surface protein of Streptococcus suis serotype 2 identified by proteomics protects mice against infection.
abstract: Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection.
authors.affiliation: The Elie Metchnikoff Department, University of Messina, Messina, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Mandanici
authors.forename: Lidia
authors.initials: L
authors.lastname: Gómez-Gascón
authors.forename: Manuela
authors.initials: M
authors.lastname: Garibaldi
authors.forename: Alfonso
authors.initials: A
authors.lastname: Olaya-Abril
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Luque
authors.forename: Carmen
authors.initials: C
authors.lastname: Tarradas
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mancuso
authors.forename: Salvatore
authors.initials: S
authors.lastname: Papasergi
authors.forename: J Antonio
authors.initials: JA
authors.lastname: Bárcena
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Teti
authors.forename: Concetta
authors.initials: C
authors.lastname: Beninati
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Rodríguez-Ortega
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000627:therapeutic use; D008565:Membrane Proteins / Q000276:immunology* / Q000627:therapeutic use; D051379:Mice; D040901:Proteomics; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology / Q000627:therapeutic use*; D017011:Streptococcus suis / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D022541:Streptococcal Vaccines