A surface protein of Streptococcus suis serotype 2 identified by proteomics protects mice against infection.
Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection.
Mandanici
Francesca
F
The Elie Metchnikoff Department, University of Messina, Messina, Italy.
Gómez-Gascón
Lidia
L
Garibaldi
Manuela
M
Olaya-Abril
Alfonso
A
Luque
Inmaculada
I
Tarradas
Carmen
C
Mancuso
Giuseppe
G
Papasergi
Salvatore
S
Bárcena
J Antonio
JA
Teti
Giuseppe
G
Beninati
Concetta
C
Rodríguez-Ortega
Manuel J
MJ
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000627:therapeutic use; D008565:Membrane Proteins / Q000276:immunology* / Q000627:therapeutic use; D051379:Mice; D040901:Proteomics; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology / Q000627:therapeutic use*; D017011:Streptococcus suis / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*
D001426:Bacterial Proteins; D008565:Membrane Proteins; D022541:Streptococcal Vaccines