HPLC and anti-inflammatory studies of the flavonoid rich chloroform extract fraction of Orthosiphon stamineus leaves.
The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3'-hydroxy-5,6,7,4'-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC(50) of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.
Yam
Mun Fei
MF
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia. yammunfei@yahoo.com
Lim
Vuanghao
V
Salman
Ibrahim Muhammad
IM
Ameer
Omar Ziad
OZ
Ang
Lee Fung
LF
Rosidah
Noersal
N
Abdulkarim
Muthanna Fawzy
MF
Abdullah
Ghassan Zuhair
GZ
Basir
Rusliza
R
Sadikun
Amirin
A
Asmawi
Mohd Zaini
MZ
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000627:therapeutic use; D002351:Carrageenan / Q000633:toxicity; D002725:Chloroform / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D005419:Flavonoids / Q000737:chemistry*; D008297:Male; D031342:Orthosiphon / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats
D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010936:Plant Extracts; D002725:Chloroform; D002351:Carrageenan