Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging.
We analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments.
Brasselet
Sophie
S
Institut Fresnel,CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Campus de St Jérôme, 13013 Marseille, France MOSAIC.
Aït-Belkacem
Dora
D
Gasecka
Alicja
A
Munhoz
Fabiana
F
Brustlein
Sophie
S
Brasselet
S
S
D000818:Animals; D001718:Birefringence; D003094:Collagen / Q000737:chemistry*; D003460:Crystallization; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D017711:Nonlinear Dynamics; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley
D003094:Collagen