title: Phenolic constituents isolated from Fragaria ananassa Duch. inhibit antigen-stimulated degranulation through direct inhibition of spleen tyrosine kinase activation.
abstract: We isolated eight phenolic constituents from Fragaria ananassa Duch. (strawberry) and determined their structures using 1D, 2D-NMR. Among the isolated compounds, linocinnamarin (LN), 1-O-trans-cinnamoyl-beta-d-glucopyranose (CG), and cinnamic acid (CA) exhibited antigen (Ag)-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells. In order to reveal the underlying mechanisms, we examined the effects of LN and CA on cellular responses induced by antigen stimulation. Treatment with both LN and CA markedly inhibited antigen-stimulated elevation of intracellular free Ca(2+) concentration and reactive oxygen species (ROS). Both LN and CA suppressed Ag-stimulated spleen tyrosine kinase (Syk) activation. These results indicate that inhibition of antigen-stimulated degranulation by LN and CA is mainly due to inactivation of Syk/phospholipase Cgamma (PLCgamma) pathways. Our findings suggest that LN and CA isolated from F. ananassa Duch. (strawberry) could be beneficial agents for alleviating symptoms of type I allergy.
authors.affiliation: Department of Materials Science and Technology, Faculty of Engineering, Gifu University, Gifu, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Itoh
authors.forename: Suguru
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Miho
authors.initials: M
authors.lastname: Saiki
authors.forename: Kenji
authors.initials: K
authors.lastname: Narumiya
authors.forename: Masaharu
authors.initials: M
authors.lastname: Yasuda
authors.forename: Kaneyuki
authors.initials: K
authors.lastname: Koshikawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nozawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology; D001491:Basophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D031985:Fragaria / Q000737:chemistry*; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000276:immunology*; D051381:Rats; D000072377:Syk Kinase
chemical_list: D018926:Anti-Allergic Agents; D000941:Antigens; D047908:Intracellular Signaling Peptides and Proteins; D010636:Phenols; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606898:Syk protein, rat