title: Dietary cholesterol modulates the excitability of rabbit hippocampal CA1 pyramidal neurons.
abstract: Previous work has shown high dietary cholesterol can affect learning and memory including rabbit eyeblink conditioning and this effect may be due to increased membrane cholesterol and enhanced hippocampal amyloid beta production. This study investigated whether dietary cholesterol modulates rabbit hippocampal CA1 neuron membrane properties known to be involved in rabbit eyeblink conditioning. Whole-cell current clamp recordings in hippocampal neurons from rabbits fed 2 percent cholesterol or normal chow for 8 weeks revealed changes including decreased after-hyperpolarization amplitudes (AHPs) - an index of membrane excitability shown to be important for rabbit eyeblink conditioning. This index was reversed by adding copper to drinking water - a dietary manipulation that can retard rabbit eyeblink conditioning. Evidence of cholesterol effects on membrane excitability was provided by application of methyl-beta-cyclodextrin, a compound that reduces membrane cholesterol, which increased the excitability of hippocampal CA1 neurons.
authors.affiliation: Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, WV 26506, United States. dwang@hsc.wvu.edu
authors.forename: Desheng
authors.initials: D
authors.lastname: Wang
authors.forename: Bernard G
authors.initials: BG
authors.lastname: Schreurs
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D019327:Copper Sulfate / Q000494:pharmacology; D008297:Male; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D002791:Cholesterol, Dietary; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D019327:Copper Sulfate