title: [Bacteriology of occluded nasolacrimal ducts in infants].
abstract: BACKGROUND
  Connatal lacrimal duct stenosis (cLDO) commonly causes purulent bacterial dacyrocystitis. The recommended treatment of choice is up to the sixth month of life a conservative antimicrobiological therapy. After the sixth month lacrimal duct irrigation with silicone tube intubation remains the gold standard. Our purpose was to analyse the current bacterial spectrum in cLDO and to compile a bacterial resistogram in order to specify antimicrobiological therapy.
  
  
  METHODS
  66 samples from the lacrimal duct of 6- to 16-month-old children (41 female, 25 male) were obtained by collecting the refluxing liquids with cotton wool swabs after irrigation of the lacrimal drainage system with sterile saline during lacrimal duct surgery. Cultures were incubated aerobically and anaerobically and the infectious agents were isolated. Sensitivity testing was performed for each isolate, testing 8 different commonly used local antibiotics. Data were statistically analysed using SPSS.
  
  
  RESULTS
  Cultures were positive in cLDO in 97 %, showing co-colonisation in 87 % with up to five bacterial strains. Gram-positive bacteria were seen in 72 % of the isolates in cLDO with Streptococcus pneumoniae (31 %) being the most abundant strain, followed by Staphylococcus aureus (13 %) and S. epidermidis (13 %). In 85 % of the samples at least one Gram-negative rod was present, most often Branhamella (12 %), followed by Haemophilus influenzae (11 %). Sensitivity testing revealed chloramphenicol, fusidic acid and ciprofloxacin/levofloxacin to be the most effective drugs in cLDO, whereas erythromycin and gentamycin turned out to be insufficient in treating cLDO.
  
  
  CONCLUSIONS
  Bacterial colonisation plays a crucial role in cLDO, showing a positive culture in 97 % with frequent co-colonisation of several bacterial strains (often in combination with at least one Gram-negative strain). The sampling of a microbiological probe of the lacrimal duct is recommended at least in therapy-refractory cases. Current bacteria in cLDO can be effectively treated with chloramphenicol, fusidic acid and ciprofloxacin. The commonly used antibiotics erythromycin and gentamicin are inappropriate as monotherapeutics.
authors.affiliation: Universitätsaugenklinik, Universität Münster. verena.prokosch@ukmuenster.de
authors.forename: V
authors.initials: V
authors.lastname: Prokosch
authors.forename: J-E
authors.initials: JE
authors.lastname: Prokosch
authors.forename: J
authors.initials: J
authors.lastname: Promesberger
authors.forename: S
authors.initials: S
authors.lastname: Thanos
authors.forename: T
authors.initials: T
authors.lastname: Stupp
mesh_terms: D003607:Dacryocystitis / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000382:microbiology*; D005260:Female; D006801:Humans; D007767:Lacrimal Duct Obstruction / Q000382:microbiology*; D008297:Male; D009301:Nasolacrimal Duct / Q000382:microbiology*