A convenient colony-based transformation method for high-throughput changing of plasmid hosts.
We have developed a convenient Escherichia coli transformation method, termed colony-based transformation, in which a single fresh colony of plasmid-containing donor strain is used instead of extracted plasmid to transform E. coli recipient cell. Thus the need for plasmid extraction and competent cell preparation is avoided. Additionally, a high-throughput transformation process based on this method was designed in which samples are prepared in a 96-well PCR plate and hundreds of transformations can be performed simultaneously in a thermocycler. We therefore suggest that this method may serve as a substitute of current transformation methods based on plasmid extraction.
An
Yingfeng
Y
Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang 110016, People's Republic of China.
Lv
Anguo
A
Wu
Wenfang
W
D004926:Escherichia coli / Q000235:genetics*; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids; D014169:Transformation, Bacterial