title: Enhanced expression of adenovirus encoding rhEPO assisted by BAPTA.
abstract: Infection efficiency is the key issue for gene delivery using adenovirus vector and usually unsatisfactory. In this study, recombinant adenoviruses encoding recombinant human EPO were prepared using the Adeasy system, and injected into the mammary gland of goats via the teat canal. BAPTA was used to treat the mammary gland to facilitate adenoviruses infection compared with EGTA. Milk serum was collected from the infected mammary gland and characterized by ELISAs and Western blotting. Expression level of rhEPO from the group treated by BAPTA was higher than that treated by EGTA.
authors.affiliation: Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Liu
authors.forename: Zeng-Sheng
authors.initials: ZS
authors.lastname: Han
authors.forename: Qing-Wang
authors.initials: QW
authors.lastname: Li
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Wen-Zong
authors.initials: WZ
authors.lastname: Lu
authors.forename: Wen-Ye
authors.initials: WY
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002614:Chelating Agents / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004921:Erythropoietin / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006041:Goats / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D008892:Milk / Q000378:metabolism*; D011897:Random Allocation; D011994:Recombinant Proteins
chemical_list: D002614:Chelating Agents; D011994:Recombinant Proteins; D004921:Erythropoietin; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid