Filtration assay for arachidonic acid release.
Arachidonic acid (AA) release is a central message in cell signaling. Fatty acid release is generally assessed by manual sampling of radioactivity release from cells prelabeled with a radiolabeled fatty acid. The assay is laborious, time-consuming, and susceptible to high noise. Here we present a fast and reproducible method for 96-well filter plates and cells in suspension, a method that is best suited for agonist concentration-response studies and, thus, for ligand screening. The method offers tremendous time and effort savings and enables execution of large experimental series previously unattainable for AA release studies.
Turunen
Pauli M
PM
Biochemistry and Cell Biology, Department of Veterinary Biosciences, University of Helsinki, FIN-00014 Helsinki, Finland.
Putula
Jaana
J
Kukkonen
Jyrki P
JP
D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005374:Filtration / Q000379:methods*; D007553:Isotope Labeling; D013997:Time Factors
D016718:Arachidonic Acid