title: Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection.
abstract: To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH(2)) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH(2) and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH(2) /DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.
authors.affiliation: Dept. of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
mesh_terms: D000586:Amination; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D015394:Molecular Structure; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry; D011136:Polysorbates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D011136:Polysorbates; D049452:Green Fluorescent Proteins; D004247:DNA