Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.
This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).
Holleran
John
J
Department of Biological Sciences, Technology Center for Networks and Pathways, Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Brown
Dara
D
Fuhrman
Margaret H
MH
Adler
Sally A
SA
Fisher
Gregory W
GW
Jarvik
Jonathan W
JW
D058666:Adrenergic beta-2 Receptor Agonists; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D017434:Protein Structure, Tertiary; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism
D058666:Adrenergic beta-2 Receptor Agonists; C505032:CFTR protein, human; D005456:Fluorescent Dyes; D051275:Glucose Transporter Type 4; D008565:Membrane Proteins; D018343:Receptors, Adrenergic, beta-2; C495692:SLC2A4 protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator