Characterization of collagen fibrils films formed on polydimethylsiloxane surfaces for microfluidic applications.
Type I collagen fibrillar thin films have been prepared on hydrophobic recovered poly(dimethylsiloxane) (PDMS) surfaces and inside of irreversibly sealed PDMS microfluidic devices. Fibrillar films prepared on PDMS surfaces have been characterized with optical microscopy and atomic force microscopy and compared with films prepared using more traditional bulk methods on thiol-coated gold substrates. Collagen fibril films formed after 18 h of incubation on PDMS surfaces were observed to have similar underlying film thicknesses (15 nm), fibril size (67 nm), fibril coverage (45%), and physiologically supermolecular structure when compared to films on gold substrates. Collagen fibrils formed within devices were also determined to be usable across physiologically relevant cell perfusion rates. To validate the utility of these collagen fibril thin films for cell culture applications, vascular smooth muscle cells are shown to attach to collagen fibrils and exhibit cell spread areas equivalent to those seen on collagen fibrils created via bulk cell culture methods on thiol-coated gold substrates. These results extend the use and benefits of collagen fibril thin films into microfluidic-based cellular studies.
Spurlin
Tighe A
TA
Biochemical Science Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, Maryland 20899, USA. tighe.spurlin@nist.gov
Forry
Samuel P
SP
Cooksey
Gregory A
GA
Plant
Anne L
AL
D024042:Collagen Type I / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
D024042:Collagen Type I; D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial; C013830:baysilon