Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice.
BACKGROUND
Mulberry therapies on type 2 diabetic patients or streptozotocin-induced diabetic rats have been reported to improve fasting blood glucose levels. We investigated the effects of dietary consumption of mulberry-leaf powder and purified quercetin 3-(6-malonylglucoside), the quantitatively major flavonol glycoside in mulberry leaves, on glucose and lipid metabolism in high-fat diet-induced obese mice. Male C57BL/6J mice aged 8 weeks were assigned to three groups (control, mulberry leaf powder (MLP), and quercetin 3-(6-malonylglucoside) (Q3MG)) and treated with their respective diets for 8 weeks.


RESULTS
We found that dietary supplementation of 10 g MLP kg(-1) or 1 g Q3MG kg(-1) in high-fat diet effectively suppressed blood glucose levels. We also noted increased expression of glycolysis-related genes and suppression of thiobarbituric acid reactive substances concentrations in the liver of Q3MG group compared to control mice.


CONCLUSIONS
Dietary consumption of Q3MG, the quantitatively major flavonol glycoside in mulberry leaves, improved hyperglycemia in obese mice and reduced oxidative stress in the liver.
Katsube
Takuya
T
Shimane Institute for Industrial Technology, Matsue City, Shimane 690-0816, Japan. katsubet@icv.ne.jp
Yamasaki
Masayuki
M
Shiwaku
Kuninori
K
Ishijima
Tomoko
T
Matsumoto
Ichiro
I
Abe
Keiko
K
Yamasaki
Yukikazu
Y
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects; D006019:Glycolysis / Q000235:genetics; D006027:Glycosides; D006943:Hyperglycemia / Q000150:complications / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D031625:Morus / Q000737:chemistry*; D009765:Obesity / Q000150:complications / Q000188:drug therapy / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001786:Blood Glucose; D004041:Dietary Fats; D006027:Glycosides; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C500753:quercetin 3-(6-malonylglucoside); D011794:Quercetin