[2'-aldehyde oligonucleotides: synthesis and use for affinity modification of DNA-recognizing proteins].
Oligonucleotides with 1,2-diol grouping were prepared from 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxo-ethyl]uridine 3'-phosphoramidite. The thermal stability of modified DNA duplexes and their ability to form complexes with the p50 subunit of the NF-kappaB transcription factor and (cytosine-5)-DNA methyltransferase SsoII were studied. The periodate oxidation of the l,2-diol grouping of the oligonucleotides resulted in reactive 2'-aldehyde derivatives. The opportunity of their use for the affinity modification of DNA-recognizing proteins was studied.
Khomiakova
E A
EA
Kazanova
E V
EV
Zubin
E M
EM
Kubareva
E A
EA
Molochkov
N V
NV
Riazanova
E M
EM
Oretskaia
T S
TS
D000447:Aldehydes / Q000138:chemical synthesis* / Q000737:chemistry; D015257:DNA-Cytosine Methylases / Q000737:chemistry*; D008024:Ligands; D052002:NF-kappa B p50 Subunit / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry
D000447:Aldehydes; D008024:Ligands; D052002:NF-kappa B p50 Subunit; D009841:Oligonucleotides; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases; D014529:Uridine