Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis.
Auxin regulates gene expression through direct physical interaction with TIR1/AFB receptor proteins during different processes of growth and development in plants. Here we report the contribution of auxin signaling pathway to the adaptative response against abiotic stress in Arabidopsis. Phenotypic characterization of tir1/afb auxin receptor mutants indicates a differential participation of each member under abiotic stress. In particular, tir1 afb2 and tir1 afb3 mutants resulted more tolerant to oxidative stress. In addition, tir1 afb2 showed increased tolerance against salinity measured as chlorophyll content, germination rate and root elongation compared with wild-type plants. Furthermore, tir1 afb2 displayed a reduced accumulation of hydrogen peroxide and superoxide anion, as well as enhanced antioxidant enzymes activities under stress. A higher level of ascorbic acid was detected in tir1 afb2 compared with wild-type plants. Thus, adaptation to salinity in Arabidopsis may be mediated in part by an auxin/redox interaction.
Iglesias
María José
MJ
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas, UE-CONICET-UNMdP, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600 Mar del Plata, Argentina.
Terrile
María Cecilia
MC
Bartoli
Carlos Guillermo
CG
D'Ippólito
Sebastián
S
Casalongué
Claudia Anahí
CA
D000222:Adaptation, Physiological / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D015398:Signal Transduction / Q000502:physiology*; D012965:Sodium Chloride / Q000633:toxicity
D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; C501405:TIR1 protein, Arabidopsis; C046131:auxin receptor, plant; D012965:Sodium Chloride