Diuretic activity of squamate mistletoe, Viscum angulatum.
Viscum angulatum Heyne ex DC (Viscaceae) is a leafless hemiparasitic shrub traditionally used in Asian countries for the treatment of hypertension. In the present study, the methanol extract of the whole plant was examined for diuretic activity in rats. The activity was evaluated using parameters such as urine volume after 5, 19, and 24 h and urine sodium, potassium, and chloride concentrations. The extract was further scrutinized for polyphenolic compounds and triterpenoids. The extract demonstrated a significant increase in and dose-dependent effect on urine excretion volume in comparison to the normal group in the tested range of 100-400 mg/kg. The extract demonstrated comparable saluretic and higher natriuretic activity (Na(+)/K(+)) compared to the furosemide treated group. However, the Cl(-)/Na(+) + K(+) ratio, which indicates carbonic anhydrase mediated activity, remained unaffected. HPLC and quantitative analysis of the extract revealed that polyphenolic compounds and the triterpenoid, oleanolic acid, are the major phytochemicals, and are proposed to be responsible for the observed diuretic effect. Oleanolic acid has been reported to possess diuretic activity with significant potassium loss in rats. In contrast to pure oleanolic acid, the extract demonstrated a valuable potassium-sparing effect. This suggests modulation of the diuretic effect of oleanolic acid by polyphenolics present in the extract. The observed dose-dependent potassium-sparing diuretic effect is a hereto unreported property of this plant.
Jadhav
R B
RB
Bioecology Laboratory, Department of Pharmacognosy, R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, India. ramjadhav123@yahoo.co.in
Bhatnagar
S P
SP
Surana
S J
SJ
D000818:Animals; D002712:Chlorides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004231:Diuresis / Q000187:drug effects*; D004232:Diuretics / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D011188:Potassium / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000032:analysis; D023382:Toxicity Tests, Acute; D014315:Triterpenes / Q000032:analysis; D014556:Urine / Q000737:chemistry; D028181:Viscum / Q000737:chemistry*
D002712:Chlorides; D004232:Diuretics; D010936:Plant Extracts; D014315:Triterpenes; D012964:Sodium; D011188:Potassium