title: Echinococcus multilocularis: the impact of ionizing radiation on metacestodes.
abstract: Alveolar echinococcosis is caused by the metacestode stage of the fox tapeworm Echinococcus multilocularis. Current chemotherapeutical options for the treatment of echinococcosis are not satisfactory, and novel drugs and/or other potential means of therapy are needed. E. multilocularis metacestodes are characterized by almost potentially unlimited growth, and also display other features of cancerous tumours. In this study, we exposed metacestodes that were generated in vitro to 50-100 Gy ionizing irradiation, and subsequently investigated the short-term (10-12 days post-treatment) and long-term (14 weeks post-treatment) effects. We found, that in the short-term, no release of alkaline phosphatase (EmAP) activity as a measure for potentially induced damage and loss of viability could be detected, and that the protein expression pattern and protease activities in vesicle fluids and medium supernatants did not alter dramatically following irradiation. However, irradiation was associated with distinct morphological and ultrastructural alterations in the tissue of metacestodes, affecting most notably cell-cell contacts, mitochondrial shape, glycogen-storage cells and lipid droplet formation. These could be detected already at 10 days following treatment and remained as such also in the long-term. In addition, as determined after 14 weeks of culture, irradiation affected the proliferation and the growth of E. multilocularis metacestodes. Thus, we demonstrate that radiotherapy does not have a clear-cut parasitocidal effect, but can lead to metabolic impairment of E. multilocularis metacestodes, as reflected by the distinct morphological and structural alterations induced by irradiation treatment.
authors.affiliation: Institute of Radiation-Oncology, University of Duesseldorf, D-40225 Duesseldorf, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Pohle
authors.forename: Raoul
authors.initials: R
authors.lastname: Ernst
authors.forename: Colin
authors.initials: C
authors.lastname: MacKenzie
authors.forename: Martin
authors.initials: M
authors.lastname: Spicher
authors.forename: Thomas
authors.initials: T
authors.lastname: Romig
authors.forename: Andrew
authors.initials: A
authors.lastname: Hemphill
authors.forename: Stephan
authors.initials: S
authors.lastname: Gripp
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003411:Arvicolinae; D004444:Echinococcosis, Hepatic / Q000532:radiotherapy*; D048210:Echinococcus multilocularis / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005589:Foxes; D005849:Gerbillinae; D015801:Helminth Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016622:Silver Staining
chemical_list: D015801:Helminth Proteins; D000469:Alkaline Phosphatase