[Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method].
In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex-periment.
Wang
Ji-Ying
JY
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jnwangjiying@163.com
Yu
Ying
Y
Feng
Li-Xia
LX
Wang
Huai-Zhong
HZ
Zhang
Qin
Q
D000818:Animals; D002725:Chloroform / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification*; D016678:Genome / Q000235:genetics*; D018859:Hair Follicle / Q000378:metabolism*; D019800:Phenol / Q000737:chemistry*; D013552:Swine / Q000235:genetics*
D019800:Phenol; D002725:Chloroform; D004247:DNA