Differentiation of embryonic stem cells in adult bone marrow.
Embryonic stem cells (ESCs) are a potential source of generating transplantable hematopoietic stem and progenitor cells, which in turn can serve as "seed" cells for hematopoietic regeneration. In this study, we aimed to gauge the ability of mouse ESCs directly differentiating into hematopoietic cells in adult bone marrow (BM). To this end, we first derived a new mouse ESC line that constitutively expressed the green fluorescent protein (GFP) and then injected the ESCs into syngeneic BM via intra-tibia. The progeny of the transplanted ESCs were then analyzed at different time points after transplantation. Notably, however, most injected ESCs differentiated into non-hematopoietic cells in the BM whereas only a minority of the cells acquired hematopoietic cell surface markers. This study provides a strategy for evaluating the differentiation potential of ESCs in the BM micro-environment, thereby having important implications for the physiological maintenance and potential therapeutic applications of ESCs.
Li
Yueying
Y
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
He
Jing
J
Wang
Fengchao
F
Ju
Zhenyu
Z
Liu
Sheng
S
Zhang
Yu
Y
Kou
Zhaohui
Z
Liu
Yanfeng
Y
Cheng
Tao
T
Gao
Shaorong
S
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice