title: Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analysis.
abstract: In this study, the concept of size-selective proteolysis was first described by using the mesoporous silica-based trypsin nanoreactor. For analysis of a complex protein sample, low-MW proteins were preferentially digested for identification while high-MW proteins were excluded from digestion.
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
authors.forename: Qianhao
authors.initials: Q
authors.lastname: Min
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Qin
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D020543:Proteome; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D014357:Trypsin