Influence of glutathione s-transferase on the ototoxicity caused by aminoglycosides.
The process of hair cell damage and death as a result of exposure to noise and ototoxins seems to be mediated by reactive oxygen species.


OBJECTIVE
To investigate the relationship between genetic polymorphisms in the Glutathione S-transferase and the susceptibility to hearing loss induced by aminoglycosides.


METHODS
Null genotypes were analyzed by multiplex-PCR in the DNA samples from 50 patients and 72 controls. The patients were divided into 3 groups, 10 with hearing loss using aminoglycosides (group A), 20 with hearing loss without exposure to the drug (group B) and 20 hearing individuals who used the antibiotic (group C).


METHODS
Experimental.


RESULTS
Polymorphisms in the GSTM1 and GSTT1 genes were found in 16% and 42% of patients and in 18% and 53% of the control group, respectively. After statistical analysis no significant difference was observed between the control groups and A (p=0.86) and (p=0.41), controls and B (p=0.27) and (p=0.24), controls and C (p=0.07) and (p=0.47), controls and A + C (p=0.09) and (p=0.47), C and A (p=0.32) and (p=0.75), GSTT1 and GSTM1, respectively.


CONCLUSIONS
Our data show that polymorphisms in GSTM1 and GSTT1 genes have no influence on the ototoxicity of aminoglycosides.
Palodetto
Bruna
B
Hermínio Ometo University, Brazil.
Postal
Mariana
M
Grignoli
Carlos Roberto Escrivão
CR
Sartorato
Edi Lúcia
EL
Oliveira
Camila Andréa de
CA
D000617:Aminoglycosides / Q000009:adverse effects*; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D034381:Hearing Loss / Q000139:chemically induced* / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D000617:Aminoglycosides; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1