title: Planosporangium mesophilum sp. nov., isolated from rhizosphere soil of Bletilla striata.
abstract: A Gram-stain-positive, non-motile actinomycete, designated strain YIM 48875(T), was isolated from rhizosphere soil of Bletilla striata and its taxonomic position was established by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 48875(T) belonged to the genus Planosporangium, supported by a bootstrap value of 100 %. Cells of strain YIM 48875(T) showed two kinds of sporangia, which also supported its classification in the genus Planosporangium. Strain YIM 48875(T) grew optimally at 28 °C, at pH 6.0-8.0 and in the presence of 2 % (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain YIM 48875(T) and Planosporangium flavigriseum YIM 46034(T) was 98.6 %. Strain YIM 48875(T) exhibited a quinone system with menaquinones MK-9(H(4)), MK-9(H(6)) and MK-9(H(8)) as the predominant compounds, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol mannoside and the major fatty acids iso-C(15 : 0) and iso-C(16 : 0); these data were markedly different from those for P. flavigriseum YIM 46034(T). The level of DNA-DNA relatedness between strain YIM 48875(T) and P. flavigriseum YIM 46034(T) was 45.5 %. It is apparent from the genotypic and phenotypic data that strain YIM 48875(T) represents a novel species of the genus Planosporangium, for which the name Planosporangium mesophilum sp. nov. is proposed. The type strain is YIM 48875(T) ( = CCTCC AA 209049(T)  = KCTC 19779(T)).
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.|College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Cao
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Rong-Xian
authors.initials: RX
authors.lastname: Jin
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
authors.forename: Hang-Xian
authors.initials: HX
authors.lastname: Lai
authors.affiliation: College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: He
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D029595:Orchidaceae / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride