In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit.
The alpha and beta tubulins compose the microtubule cytoskeleton which is involved in many cellular processes such as vesicular transport. The photoreceptor cells in the retina are neurons specialized for phototransduction. Here we report a novel interaction between tubulin and the photoreceptor cGMP phosphodiesterase (PDE6) gamma subunit (PDE gamma). The specificity and molecular details of the PDE gamma:tubulin interaction were analyzed through the experiments of pull down, microtubule co-sedimentation, and NMR spectroscopy. The tubulin-interacting site was identified to be in the PDE gamma C-terminal I67-G85 region, and the interaction interface appeared to be distinct from those with the other PDE gamma targets in phototransduction. We also observed that PDE gamma interacted with tubulin in a GTP-dependent manner. Our findings offer implications for non-phototransduction role(s) of PDE gamma in the photoreceptor neurons.
Chu
Uyen B
UB
Department of Pharmacology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
Song
Jikui
J
Mavlyutov
Timur A
TA
Guo
Lian-Wang
LW
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*
D014404:Tubulin; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6