Production of erythroid cells from human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPS).
Erythroid progenitors can be generated ex vivo from human embryonic stem cells (hESC) or human induced pluripotent stem cells (hiPS). Development of laboratory scale culture conditions capable of generating mature functional erythrocytes from human embryonic stem cells or human induced pluripotent stem cells would open the possibility for manufacture of therapeutic quantities of red cells and thereby new clinical transfusion products. Current attempts to produce erythrocytes from human embryonic stem cells reveal the need for greater understanding of the process whereby primitive erythropoiesis switches to definitive fetal and adult erythropoiesis and the factors driving erythrocyte maturation. Studies with human embryonic stem cells have already yielded encouraging results but functional mature biconcave erythrocytes have yet to be generated from these cells.
Anstee
D J
DJ
Bristol Institute for Transfusion Sciences, NHSBT, Filton, Bristol BS34 7QG, UK. david.anstee@nhsbt.nhs.uk
D000328:Adult; D001803:Blood Transfusion / Q000379:methods; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D016207:Cytokines / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D004900:Erythroblasts / Q000166:cytology / Q000502:physiology; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D041905:Erythroid Cells / Q000166:cytology*; D004920:Erythropoiesis / Q000502:physiology*; D005312:Fetal Blood / Q000166:cytology / Q000502:physiology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*; D012156:Reticulocytes / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
D016207:Cytokines