Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein.
To characterize the humoral response to the unglycosylated central region of the respiratory syncytial virus (RSV) attachment (G) protein, we generated glutathione S-transferase (GST)-RSV G subdomains (central core (CC), residues 151-190; proximal central core (PCC), 151-172; and distal central core (DCC), 173-190) to screen paired sera from RSV subtype A- or B-infected adults in hospitalized or outpatient settings. Following RSV infection, a >or=4-fold increase in homo- and heterosubtypic IgG response was noted in most subjects against the RSV G CC and PCC regions; in contrast, such titer increases against the RSV G DCC was only noted in a homosubtypic manner. Our results have implications for RSV G-based serological diagnostics and vaccine development.
Murata
Yoshihiko
Y
Division of Infectious Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry and Infectious Diseases Unit, Rochester General Hospital, 601 Elmwood Avenue Box 689, Rochester, NY 14642, USA. Yoshihiko Murata@urmc.rochester.edu
Lightfoote
Paula M
PM
Biear
Jamie N
JN
Falsey
Ann R
AR
Walsh
Edward E
EE
D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D056945:Hep G2 Cells; D006801:Humans; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000276:immunology; D008875:Middle Aged; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D022261:Respiratory Syncytial Virus Vaccines; D014759:Viral Envelope Proteins; C116204:attachment protein G