The polyphenolics in the aqueous extract of Psidium guajava kinetically reveal an inhibition model on LDL glycation.
Guava [Psidium guajava L. (Myrtaceae)] budding leaf extract (PE) has shown tremendous bioactivities. Previously, we found seven major compounds in PE, i.e., gallic acid, catechin, epicatechin, rutin, quercetin, naringenin, and kaempferol. PE showed a potentially active antiglycative effect in an LDL (low density lipoprotein) mimic biomodel, which can be attributed to its large content of polyphenolics. The glycation and antiglycative reactions showed characteristic distinct four-phase kinetic patterns. In the presence of PE, the kinetic coefficients were 0.000438, 0.000060, 0.000, and -0.0001354 ABS-mL/mg-min, respectively, for phases 1 to 4. Computer simulation evidenced the dose-dependent inhibition model. Conclusively, PE contains a large amount of polyphenolics, whose antiglycative bioactivity fits the inhibition model.
Chen
Kuan-Chou
KC
Department of Urology, Taipei Medical University Shuang-Ho Hospital, Taipei, Taiwan.
Chuang
Chao-Ming
CM
Lin
Li-Yun
LY
Chiu
Wen-Ta
WT
Wang
Hui-Er
HE
Hsieh
Chiu-Lan
CL
Tsai
Tsuimin
T
Peng
Robert Y
RY
D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000493:pharmacokinetics*; D017127:Glycation End Products, Advanced; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008954:Models, Biological; D010636:Phenols / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000302:isolation & purification / Q000493:pharmacokinetics*; D018515:Plant Leaves; D059808:Polyphenols; D031647:Psidium; D014867:Water / Q000378:metabolism*; D000093844:Glycated Proteins
D005419:Flavonoids; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins