The expression patterns of minor fibrillar collagens during development in zebrafish.
Minor fibrillar collagens are recognized as the organizers and nucleators during collagen fibrillogenesis but likely serve additional functions. The minor fibrillar collagens include collagens type V and XI. Mutations of collagens type V and XI can cause Ehlers-Danlos, Stickler's, and Marshall's syndromes in human. We have characterized the spatiotemporal expression patterns of Col11a1, Col11a2, Col5a1 as well as Col5a3 in zebrafish embryos by in situ hybridization. Col5a1 is expressed in developing somites, neural crest, the head mesenchyme, developing cranial cartilage, pharyngeal arches and vertebrae. Col5a3 is detected in the notochord, mesenchyme cells in the eyes and lens. Both Col11a1 and Col11a2 have similar expression patterns, including notochord, otic vesicle, and developing cranial cartilages. Zebrafish may therefore serve as a valuable vertebrate model system for the study of diseases associated with collagens type V and XI mutations.
Fang
Ming
M
Department of Biological Sciences, Boise State University, Boise, ID, USA.
Adams
Jason S
JS
McMahan
B Lane
BL
Brown
Raquel J
RJ
Oxford
Julia Thom
JT
D000595:Amino Acid Sequence; D000818:Animals; D001934:Branchial Region / Q000378:metabolism; D004195:Disease Models, Animal; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism; D005123:Eye / Q000196:embryology / Q000378:metabolism; D024022:Fibrillar Collagens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology; D009432:Neural Crest / Q000378:metabolism; D009672:Notochord / Q000378:metabolism; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D019170:Somites / Q000378:metabolism; D013131:Spine / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D024022:Fibrillar Collagens; D029961:Zebrafish Proteins