title: Transplanted mesenchymal stem cells with pure fibrinous gelatin-transforming growth factor-beta1 decrease rabbit intervertebral disc degeneration.
abstract: BACKGROUND
  Disc degeneration is a major reason for low back pain and can be caused by apoptosis. The prevention of apoptosis using mesenchymal stem cells (MSCs) may lead to new treatments for low back pain. Previous studies have reported that transplanted MSCs can proliferate and differentiate into cells expressing some of the major phenotypic qualities of nucleus pulposus cells. However, the effects of MSC transplantation on the disc height index (DHI) and apoptosis inhibition have not yet been thoroughly investigated.
  
  
  OBJECTIVE
  The present study evaluates the effects of MSC transplantation on DHI and its potential to inhibit apoptosis.
  
  
  METHODS
  Random, controlled, animal experiment study.
  
  
  METHODS
  The annulus fibrosus of 54 white New Zealand rabbits was punctured with a 21-gauge needle, and the nucleus pulposus tissue from the intervertebral discs was aspirated. The degenerative disc model was produced in each rabbit, which were then randomly divided into three groups: degenerative model group; pure fibrinous gelatin-transforming growth factor-beta1 (PFG-TGF-beta1) transplanted group; and MSC-PFG-TGF-beta1 transplanted group. Computed radiography imaging, magnetic resonance imaging, and histological examinations were performed at Weeks 4, 8, and 12.
  
  
  RESULTS
  The transplanted MSCs inhibited apoptosis and slowed the rate of decrease in DHI. Magnetic resonance imaging results showed that the MSC-PFG-TGF-beta1 group had less degeneration and a slower decrease in DHI compared with both the degenerative model and PFG-TGF-beta1 groups. An increased quantity of nucleus pulposus and type II collagen content and a decrease in the rate of cell apoptosis were noted in the MSC-PFG-TGF-beta1 group.
  
  
  CONCLUSIONS
  Mesenchymal stem cells can slow the rate at which the DHI decreases. This effect may be because of the inhibition of apoptosis by MSCs.
authors.affiliation: The First Affiliated Hospital of Suzhou University, Suzhou 215006, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Jiayong
authors.initials: J
authors.lastname: Liu
authors.forename: Molly
authors.initials: M
authors.lastname: Ebraheim
authors.forename: Sharmaine
authors.initials: S
authors.lastname: Castillo
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Liu
authors.forename: Tiansi
authors.initials: T
authors.lastname: Tang
authors.forename: Nabil A
authors.initials: NA
authors.lastname: Ebraheim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D024043:Collagen Type II / Q000096:biosynthesis; D005780:Gelatin; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007403:Intervertebral Disc / Q000473:pathology / Q000502:physiology; D055959:Intervertebral Disc Degeneration / Q000601:surgery*; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D011817:Rabbits; D014057:Tomography, X-Ray Computed; D053773:Transforming Growth Factor beta1 / Q000627:therapeutic use*
chemical_list: D024043:Collagen Type II; D053773:Transforming Growth Factor beta1; D005780:Gelatin