Direct profiling of saccharides, organic acids and anthocyanins in fruits using electrospray droplet impact/secondary ion mass spectrometry.
Electrospray droplet impact (EDI)/secondary ion mass spectrometry (SIMS) is a new desorption/ionization technique for mass spectrometry in which highly charged water clusters produced from atmospheric-pressure electrospray are accelerated in vacuum by several kV and impact on the sample deposited on the metal substrate. In this study, we applied EDI/SIMS directly to fruits, such as bananas, strawberries, grapes and apples. The major components in the fruits--fructose, glucose, sucrose and organic acids--could be observed with strong signal intensities. EDI/SIMS was also applied to the analysis of different regions of strawberries and apples. Compared with matrix-assisted laser desorption/ionization (MALDI), ion signals with lower background signals could be obtained, particularly for the low molecular weight analytes.
Asakawa
Daiki
D
Clean Energy Research Center, The University of Yamanashi, Takeda 4-3-11, Kofu, Yamanashi, 400-8511, Japan.
Hiraoka
Kenzo
K
D000143:Acids / Q000032:analysis*; D000872:Anthocyanins / Q000032:analysis*; D031985:Fragaria / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D027845:Malus / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis*; D028521:Musa / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D013395:Sucrose / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods; D027843:Vitis / Q000737:chemistry
D000143:Acids; D000872:Anthocyanins; D009005:Monosaccharides; D013395:Sucrose