Inherited neuroaxonal dystrophy in dogs causing lethal, fetal-onset motor system dysfunction and cerebellar hypoplasia.
Neuroaxonal dystrophy in brainstem, spinal cord tracts, and spinal nerves accompanied by cerebellar hypoplasia was observed in a colony of laboratory dogs. Fetal akinesia was documented by ultrasonographic examination. At birth, affected puppies exhibited stereotypical positioning of limbs, scoliosis, arthrogryposis, pulmonary hypoplasia, and respiratory failure. Regional hypoplasia in the central nervous system was apparent grossly, most strikingly as underdeveloped cerebellum and spinal cord. Histopathologic abnormalities included swollen axons and spheroids in brainstem and spinal cord tracts; reduced cerebellar foliation, patchy loss of Purkinje cells, multifocal thinning of the external granular cell layer, and loss of neurons in the deep cerebellar nuclei; spheroids and loss of myelinated axons in spinal roots and peripheral nerves; increased myocyte apoptosis in skeletal muscle; and fibrofatty connective tissue proliferation around joints. Breeding studies demonstrated that the canine disorder is a fully penetrant, simple autosomal recessive trait. The disorder demonstrated a type and distribution of lesions homologous to that of human infantile neuroaxonal dystrophy (INAD), most commonly caused by mutations of phospholipase A2 group VI gene (PLA2G6), but alleles of informative markers flanking the canine PLA2G6 locus did not associate with the canine disorder. Thus, fetal-onset neuroaxonal dystrophy in dogs, a species with well-developed genome mapping resources, provides a unique opportunity for additional disease gene discovery and understanding of this pathology.
Fyfe
John C
JC
Laboratory of Comparative Medical Genetics, Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, USA. fyfe@cvm.msu.edu
Al-Tamimi
Raba' A
RA
Castellani
Rudy J
RJ
Rosenstein
Diana
D
Goldowitz
Daniel
D
Henthorn
Paula S
PS
D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000002:abnormalities / Q000473:pathology / Q000503:physiopathology; D002526:Cerebellar Diseases / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D002531:Cerebellum / Q000002:abnormalities / Q000473:pathology / Q000503:physiopathology; D004283:Dog Diseases / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D004285:Dogs; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D008297:Male; D019150:Neuroaxonal Dystrophies / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D010375:Pedigree; D011247:Pregnancy
D005819:Genetic Markers