Genotoxic, physiological and immunological effects caused by temperature increase, air exposure or food deprivation in freshwater crayfish Astacus leptodactylus.
The aim of this research was to investigate influence of different environmental stressors, such as temperature increase, air exposure and food deprivation on DNA integrity of a bioindicator species, freshwater crayfish Astacus leptodactylus. DNA damage was measured in crayfish haemocytes using Comet assay and micronucleus test. Crayfish haemolymph was subsequentially sampled during their 7 days of exposure to increased temperatures (25 and 30 degrees C) and during 24 h of air exposure. Both groups were also monitored through the following 7 days of recovery period. Food deprived crayfish were monitored over a period of 2 weeks. Alterations of measured physiological and immunological haemolymph parameters (THC, lactate, glucose and protein concentration) indicated stress response in exposed crayfish. However, only the stress induced by increased temperature significantly increased DNA damage in freshwater crayfish while food deprivation or air exposure did not cause a significant genotoxic effect.
Malev
Olga
O
School of Environmental Sciences, University of Nova Gorica, Rozna Dolina, 5000 Nova Gorica, Slovenia.
Srut
Maja
M
Maguire
Ivana
I
Stambuk
Anamaria
A
Ferrero
Enrico A
EA
Lorenzon
Simonetta
S
Klobucar
Göran I V
GI
D000388:Air; D000818:Animals; D003400:Astacoidea / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002452:Cell Count; D004249:DNA Damage; D005508:Food Deprivation; D005618:Fresh Water / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009152:Mutagenicity Tests; D013312:Stress, Physiological; D013696:Temperature
D019344:Lactic Acid; D005947:Glucose