Redundant and unique roles of coronin proteins in Dictyostelium.
Dictyostelium discoideum harbors a short (CRN12) and a long coronin (CRN7) composed of one and two beta-propellers, respectively. They are primarily present in the cell cortex and cells lacking CRN12 (corA⁻) or CRN7 (corB⁻) have defects in actin driven processes. We compared the characteristics of a mutant cell line (corA⁻/corB⁻) lacking CRN12 and CRN7 with the single mutants focusing on cytokinesis, phagocytosis, chemotaxis and development. Cytokinesis, uptake of small particles, and developmental defects were not enhanced in the corA⁻/corB⁻ strain as compared to the single mutants, whereas motility and phagocytosis of yeast particles were more severely impaired. It appears that although both proteins affect the same processes they do not act in a redundant manner. Rather, they often act antagonistically, which is in accordance with their proposed roles in the actin cytoskeleton where CRN12 acts in actin disassembly whereas CRN7 stabilizes actin filaments and protects them from disassembly.
Shina
Maria C
MC
Institute for Biochemistry I, Center for Molecular Medicine Cologne, University of Cologne, Germany.
Müller-Taubenberger
Annette
A
Unal
Can
C
Schleicher
Michael
M
Steinert
Michael
M
Eichinger
Ludwig
L
Müller
Rolf
R
Blau-Wasser
Rosemarie
R
Glöckner
Gernot
G
Noegel
Angelika A
AA
D000199:Actins / Q000378:metabolism; D000818:Animals; D002633:Chemotaxis / Q000502:physiology; D048749:Cytokinesis / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli; D017353:Gene Deletion; D016952:Legionella pneumophila; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D014162:Transfection
D000199:Actins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C072061:coronin proteins