[Evidence for the existence of ATP in the marginal cells of the neonatal rat cochlea].
OBJECTIVE
To study the evidence of adenosine triphosphate (ATP) in the marginal cells of the stria vascularis in the neonatal rat cochlea, namely, whether ATP vesicles could be detected in the marginal cells and ATP release could be detected in the culture solution in vitro.


METHODS
The marginal cells of 1-3 day old Sprague-Dawley rats were cultivated, purified and verified. ATP vesicles Were observed in the marginal cells under fluorescence microscope using quinacrine staining technique. The concentration of ATP released from the cells in the extracellular solution was determined through bioluminescence assay.


RESULTS
The marginal cells were verified in vitro by detecting antibodies of cytokeratin and vimentin using flow cytometry. A large number of anterior-like staining could be observed in cultured marginal cells that were incubated with quinacrine. The concentration of ATP released from the cells in the culture solution could be determined, and the concentration of ATP could be calculated by fluorescent intensity.


CONCLUSIONS
The ATP vesicles exist in the marginal cells and can release ATP.
Chen
Jie
J
Department of Otolaryngology, Medical College of Shanghai Jiaotong University, Shanghai, 200127, China.
He
Shan
S
Yang
Jun
J
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013316:Stria Vascularis / Q000166:cytology*
D000255:Adenosine Triphosphate