Fiber phenotype and coenzyme Q₁₀ content in Turkey skeletal muscles.
Phenotypical differences between muscle fibers are associated with a source of cellular energy. Coenzyme Q(10) (CoQ(10)) is a major component of the mitochondrial oxidative phosphorylation process, and it significantly contributes to the production of cellular energy in the form of ATP. The objective of this study was to determine the relationship between whole-tissue CoQ(10) content, mitochondrial CoQ(10) content, mitochondrial protein, and muscle phenotype in turkeys. Four specialized muscles (anterior latissimus dorsi, ALD; posterior latissimus dorsi, PLD; pectoralis major, PM, and biceps femoris, BF) were evaluated in 9- and 20-week-old turkey toms. The amount of muscle mitochondrial protein was determined using the Bradford assay and CoQ(10) content was measured using HPLC-UV. The amount of mitochondrial protein relative to total protein was significantly lower (p < 0.05) at 9 compared to 20 weeks of age. All ALD fibers stained positive for anti-slow (S35) MyHC antibody. The PLD and PM muscle fibers revealed no staining for slow myosin heavy chain (S35 MyHC), whereas half of BF muscle fibers exhibited staining for S35 MyHC at 9 weeks and 70% at 20 weeks of age. The succinate dehydrogenase (SDH) staining data revealed that SDH significantly increases (p < 0.05) in ALD and BF muscles and significantly decreases (p < 0.05) in PLD and PM muscles with age. The study reveals age-related decreases in mitochondrial CoQ(10) content in muscles with fast/glycolytic profile, and demonstrates that muscles with a slow/oxidative phenotypic profile contain a higher proportion of CoQ(10) than muscles with a fast/glycolytic phenotypic profile.
Nierobisz
L S
LS
Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA. lsnierob@ncsu.edu
Hentz
N G
NG
Felts
J V
JV
Mozdziak
P E
PE
D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D007150:Immunohistochemistry; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014422:Turkeys / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D014451:Ubiquinone; D013385:Succinate Dehydrogenase; D009218:Myosins; C024989:coenzyme Q10