[Biochemical changes in striatum of Parkinson's disease rat model observed by modified proton magnetic resonance spectroscopy].
This study was undertaken to observe the biochemical changes in striatum of Parkinson's disease (PD) rat model by modified proton magnetic resonance spectroscopy. 12 SD rats were divided into model (n=7) and control (n=5) groups. At 3 weeks after the injection of 6-hydroxydopamine into right striatum, 1H-MRS on the striatum was taken by modified proton magnetic resonance spectroscopy, and then tyrosine hydroxylase (TH) immunostatining was used to visualize the changes of the neurons in substantia nigra and neurites in striatum. The results showed that TH positive neurons and neurites in the substantia nigra compacts (SNc) and striatum in the normal side of the rat model of PD were decreased (P < 0.05), which proved the successful establishment of PD models. The NAA/Cr ratio of the injected side striatum of model group was lower than that of the normal side (P < 0.05). The ratios of Cho/Cr showed no significant difference between the two sides (P > 0.05). These results indicated that the modified 1.5T 1H-MRS should be a noninvasive technique which could provide useful information about the biochemical metabolites in striatum for the study of PD in rat model.
Zheng
Zhijuan
Z
Department of Histology and Embryology, Weifang Medical College, Weifang 261053, China.
Fu
Wenyu
W
Wang
Junqing
J
Sun
Xihe
X
Zhuang
Wenxin
W
Lu
E
E
Yang
Li
L
Lu
Cui
C
D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology*; D005260:Female; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase