title: Facilitation of Ih channels by P2Y1 receptors activation in Mesencephalic trigeminal neurons.
abstract: P2Y(1) receptors, a subset of G-protein coupled receptors, have been shown to participate in sensory transduction in the periphery nervous system. However, little is known about their sensory function in the central nervous system. Here, by using immunohistochemistry, we showed that P2Y(1) receptors are predominantly localized in the somata of Mesencephalic trigeminal neurons (Mes V neurons), the primary sensory neurons in brainstem. Whole-cell voltage-clamp recording revealed that ADP-beta-S, a P2Y receptor agonist, enhanced the activity of hyperpolarization-activated cation channels (Ih channels) in Mes V neurons and that the activity-enhancing effect of ADP-beta-S could be blocked by a specific P2Y(1) receptor antagonist, MRS 2179. Taken together, these results suggested a possible role of P2Y(1) receptors in the information transduction of central sensory neurons through regulating Ih channel activities.
authors.affiliation: Department of Neurobiology, College of Basic Medical Sciences, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiu
authors.forename: Jun-an
authors.initials: JA
authors.lastname: Yan
authors.forename: Wen-juan
authors.initials: WJ
authors.lastname: He
authors.forename: Yan-dong
authors.initials: YD
authors.lastname: Zhao
authors.forename: Zhi-an
authors.initials: ZA
authors.lastname: Hu
authors.forename: Huai-zhen
authors.initials: HZ
authors.lastname: Ruan
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000502:physiology*; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D066298:In Vitro Techniques; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D058912:Purinergic P2Y Receptor Agonists / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D058472:Receptors, Purinergic P2Y1 / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology; D014278:Trigeminal Nuclei / Q000378:metabolism*
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; C111914:N(6)-methyl-2'-deoxyadenosine 3',5'-diphosphate; D015221:Potassium Channels; D058912:Purinergic P2Y Receptor Agonists; D058472:Receptors, Purinergic P2Y1; D013873:Thionucleotides; C030812:adenosine 5'-O-(2-thiodiphosphate); D000244:Adenosine Diphosphate