title: Heavy metal tolerant Metalliresistens boonkerdii gen. nov., sp. nov., a new genus in the family Bradyrhizobiaceae isolated from soil in Thailand.
abstract: Bacterial strains from inoculated soybean field soil in Thailand were directly isolated using Bradyrhizobium japonicum selective medium (BJSM), on the basis of Zn(2+) and Co(2+) resistance of B. japonicum and B. elkanii. The isolates were classified into symbiotic and non-symbiotic groups by inoculation assays and Southern hybridization of nod and nif genes. In this study, a nearly full-length 16S rRNA gene sequence showed that the non-symbiotic isolates were more closely related to members of Rhodopseudomonas and to a number of uncultured bacterial clones than to members of Bradyrhizobium. Therefore, a polyphasic study was performed to determine the taxonomic positions of four representatives of the non-symbiotic isolates. Multilocus phylogenetic analysis of individual genes and a combination of the 16S rRNA and three housekeeping genes (atpD, recA and glnII) supported the placement of the non-symbiotic isolates in a different genus. The ability of heavy metal resistance in conjunction with phenotypic analyses, including cellular fatty acid content and biochemical characteristics, showed that the non-symbiotic isolates were differentiated from the other related genera in the family Bradyrhizobiaceae. Therefore, the non-symbiotic isolates represented a novel genus and species, for which the name Metalliresistens boonkerdii gen. nov., sp. nov. is proposed. The type strain is NS23 (= NBRC 106595(T)=BCC 40155(T)).
authors.affiliation: Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, Nakhonrachasima 30000, Thailand.
authors.forename: Rujirek
authors.initials: R
authors.lastname: Noisangiam
authors.forename: Achara
authors.initials: A
authors.lastname: Nuntagij
authors.forename: Neelawan
authors.initials: N
authors.lastname: Pongsilp
authors.forename: Nantakorn
authors.initials: N
authors.lastname: Boonkerd
authors.forename: Jessada
authors.initials: J
authors.lastname: Denduangboripant
authors.forename: Clive
authors.initials: C
authors.lastname: Ronson
authors.forename: Neung
authors.initials: N
authors.lastname: Teaumroong
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D041841:Bradyrhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000737:chemistry; D020459:Genes, rRNA; D019216:Metals, Heavy / Q000494:pharmacology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology; D013045:Species Specificity; D013559:Symbiosis; D013785:Thailand; D015032:Zinc / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D003035:Cobalt; D015032:Zinc