title: Repair of the canine vertebral lamina with a combination of autologous micromorselized bone and poly-lactic acid gel after a total laminectomy.
abstract: The complications of a total laminectomy can include epidural scar adhesion, secondary vertebral canal stenosis and lumbar instability. The aim of this study was to assess the ability of a combination of autologous micromorselized bone and polylactic acid (PLA) gel to stimulate bone repair within canine vertebral lamina defects, as well as to prevent peridural adhesion following a laminectomy. Twenty dogs underwent a L1, L3 and L5 laminectomy after a pre-computed tomography examination. The 60 laminectomies were divided into 4 groups, and underwent the following treatments: Group A were treated with a combination of autologous micromorselized bone and PLA gel (n = 16); Group B were treated with micromorselized bone only (n = 16); Group C with PLA gel only (n = 14); and Group D were left untreated as they were the blank controls (n = 14). Vertebral laminae were harvested at 4th and 8th weeks, and examined by computed tomography and histological staining. Significant bone regeneration was observed at 4 and 8 weeks in Groups A and B, although the newly formed bone in the latter group was more irregular in shape. Spinal canal cross-sectional areas in Group B decreased as compared with those in Group A(p < 0.05), and the degree of peridural adhesion in Groups A and C was significantly lower compared with Groups B and D (p < 0.05). These findings indicate that a combination of micromorselized bone and PLA gel may provide a better strategy for the treatment of spinal stenosis.
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Chuang
authors.forename: Chih-Peng
authors.initials: CP
authors.lastname: Chang
authors.forename: Hung-Tau
authors.initials: HT
authors.lastname: Shen
authors.forename: Jiun
authors.initials: J
authors.lastname: Kao
authors.forename: Jing-Lung
authors.initials: JL
authors.lastname: Yan
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000627:therapeutic use*; D016025:Bone Transplantation; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000627:therapeutic use*; D007796:Laminectomy / Q000009:adverse effects*; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000601:surgery*; D008297:Male; D011091:Polyesters; D011108:Polymers / Q000627:therapeutic use*; D011183:Postoperative Complications / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D013122:Spinal Diseases / Q000601:surgery*
chemical_list: D001843:Bone Cements; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)