Identification and characterization of a new monoamine oxidase type C-like dehydratase from Phytophthora capsici involved in polyhydroxyalkanoates biosynthesis.
A new monoamine oxidase type C-like dehydratase gene (MaoC) supplying (R)-3-hydroxyacyl-CoA from the fatty acid oxidation pathway to polyhydroxyalkanoates (PHAs) biosynthetic pathways was identified from Phytophthora capsici. MaoC was over-expressed in Escherichia coli and the recombinant MaoC was purified. The purified tagged MaoC shows the enoyl-CoA hydratase activity of 58 U/mg towards crotonyl-CoA. MaoC may not fold properly above 40°C which was revealed by circular dichroism analysis. Crystal of MaoC diffracts to 1.93 with unit-cell parameters of a = 81.458 Å, b = 82.614 Å, c = 124.228 [corrected] Å, α = β = γ = 90°.
Wang
Huizheng
H
Department of Plant Pathology, Shandong Agricultural University, Tai'an 271018, China.
Guo
Jiubiao
J
Pang
Hai
H
Zhang
Xiuguo
X
D000214:Acyl Coenzyme A / Q000378:metabolism; D002942:Circular Dichroism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010838:Phytophthora / Q000201:enzymology*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013696:Temperature
D000214:Acyl Coenzyme A; D004271:DNA, Fungal; D005227:Fatty Acids; D054813:Polyhydroxyalkanoates; D011994:Recombinant Proteins; C010701:crotonyl-coenzyme A; D008995:Monoamine Oxidase; D006836:Hydro-Lyases