S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization.
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37 degrees C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
Ccahuana-Vásquez
Renzo Alberto
RA
Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
Cury
Jaime Aparecido
JA
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002417:Cattle; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003731:Dental Caries / Q000517:prevention & control; D009055:Mouth / Q000382:microbiology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000254:growth & development*; D013395:Sucrose / Q000378:metabolism; D013549:Sweetening Agents / Q000378:metabolism; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control*
D000890:Anti-Infective Agents; D013549:Sweetening Agents; D013395:Sucrose; D012969:Sodium Fluoride; D002710:Chlorhexidine