Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis.
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.
Tong
Steven Y C
SY
Menzies School of Health Research, Darwin, PO Box 41096 Casuarina, NT 0811, Australia. Steven.tong@menzies.edu.au
Lilliebridge
Rachael A
RA
Holt
Deborah C
DC
Coombs
Geoffrey W
GW
Currie
Bart J
BJ
Giffard
Philip M
PM
D001315:Australia; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020033:Protein Isoforms / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D044366:Transition Temperature
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D020033:Protein Isoforms