Antimicrobial activity investigation on Wuyiencin fractions of different polarity.
The aim of this study was to evaluate the antimicrobial activity of Wuyiencin fractions with different polarities against six indicator microorganisms: Rhodotorula rubra, Bacillus subtilis, Bacillus megaterium, Escherichia coli, Cladosporium fulvum and Staphylococcus aureus. The fermentation broth of Wuyiencin was submitted to AB-8 macroporous adsorptive resin and fractionated with solvents of different polarity. The fraction eluted with water had remarkably antimicrobial activity against all the microorganisms investigated except for C. fulvum and S. aureus (MIC <or= 0.0625 mg/mL), probably due to the presence of active components. The fraction eluted with methanol showed potential antimicrobial activity against all the test microorganisms except for R.rubra, with MIC values of 0.5 and 2 mg/mL. In conclusion, fractions eluted with water and methanol, respectively, represent the main active-part of Wuyiencin, and could be emphasized for agricultural applications in the future.
Cui
Zengjie
Z
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Zhang
Kecheng
K
She
Gaimei
G
Lin
Yinni
Y
Sun
Lei
L
Cheng
Yan
Y
Tan
Beibei
B
D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000187:drug effects; D001412:Bacillus subtilis / Q000187:drug effects; D005591:Chemical Fractionation; D002963:Cladosporium / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005285:Fermentation; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012248:Rhodotorula / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D010936:Plant Extracts