Tudor-SN interacts with and co-localizes with G3BP in stress granules under stress conditions.
SGs are mRNA containing cytoplasmic structures that are assembled in response to stress. Tudor-SN protein is a ubiquitously expressed protein. Here, Tudor-SN protein was found to physiologically interact with G3BP, which is the marker and effector of SG. The kinetics of the assembly of SGs in the living cells demonstrated that Tudor-SN co-localizes with G3BP and is recruited to the same SGs in response to different stress stimuli. Knockdown of endogenous Tudor-SN did not inhibit the formation of SGs, but retarded the aggregation of small SGs into large SGs. Thus Tudor-SN may not be an initiator as essential as G3BP for the formation of SGs, but affects the aggregation of SGs. These findings identify Tudor-SN as a novel component of SGs.
Gao
Xingjie
X
Department of Immunology, Basic Medical College, Tianjin Medical University, PR China.
Ge
Lin
L
Shao
Jie
J
Su
Chao
C
Zhao
Hong
H
Saarikettu
Juha
J
Yao
Xuyang
X
Yao
Zhi
Z
Silvennoinen
Olli
O
Yang
Jie
J
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004265:DNA Helicases; D004720:Endonucleases; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D025941:Protein Interaction Mapping; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological
D002352:Carrier Proteins; D009687:Nuclear Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000067776:RNA Recognition Motif Proteins; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D004720:Endonucleases; C095221:SND1 protein, human; D004265:DNA Helicases; C116070:G3BP1 protein, human; D020365:RNA Helicases