title: Quantum yields and kinetics of the firefly bioluminescence reaction of beetle luciferases.
abstract: Quantum yields of firefly bioluminescence reactions were determined for beetle luciferases from the three main families of luminous beetles emitting different bioluminescence colors. Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). Mutant analyses of active site-substituted Pyrocoelia miyako luciferases showed that, although k(cat) was decreased by the mutations, the QY was not significantly affected.
authors.affiliation: National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology(AIST), Tsukuba, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Niwa
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Ichino
authors.forename: Shiho
authors.initials: S
authors.lastname: Kumata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hiraishi
authors.forename: Dai-Ichiro
authors.initials: D
authors.lastname: Kato
authors.forename: Vadim R
authors.initials: VR
authors.lastname: Viviani
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohmiya
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D048888:Fireflies / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors; D007700:Kinetics; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics; D049449:Luminescence
chemical_list: D008156:Luciferases