Key compound groups for the neuroprotective effect of roots of Polygonum cuspidatum on transient middle cerebral artery occlusion in Sprague-Dawley rats.
Polygonum cuspidatum is a potent anti-oxidant herb that is well known for its various bioactivities. The current study investigates which compound group is most effective, to establish the key compound groups for quality assessment, especially in terms of neuroprotective effects. The roots of P. cuspidatum were extracted with 85% methanol and fractionated with hexane, ethyl acetate, n-butanol and water. Each fraction was applied to an in vitro radical scavenging assay, a lipid peroxidation assay in brain homogenates and an in vivo assay using a transient focal cerebra ischemia model induced by a middle cerebral artery occlusion in a Sprague-Dawley rat. The ethyl acetate fraction was the most effective fraction in both in vitro and in vivo assays, having the highest stilbene and anthraquinone contents. These results suggest that stilbenes and anthraquinones may be key compound groups for the quality assessment of the anti-oxidative and neuroprotective effects of P. cuspidatum.
Kim
Jinmo
J
Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea.
Kim
Mi-Yeon
MY
Leem
Kang-Hyun
KH
Moon
Sangkwan
S
Jamakattel-Pandit
Nirmala
N
Choi
Hoyoung
H
Kim
Hocheol
H
Bu
Youngmin
Y
D020001:1-Butanol; D000085:Acetates; D000818:Animals; D000880:Anthraquinones / Q000032:analysis; D002561:Cerebrovascular Disorders / Q000188:drug therapy*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D045468:Fallopia japonica / Q000737:chemistry*; D006586:Hexanes; D015227:Lipid Peroxidation; D008297:Male; D018696:Neuroprotective Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D045442:Rotarod Performance Test; D013267:Stilbenes / Q000032:analysis
D000085:Acetates; D000880:Anthraquinones; D006586:Hexanes; D018696:Neuroprotective Agents; D010936:Plant Extracts; D013267:Stilbenes; C007650:ethyl acetate; D020001:1-Butanol