Regulation of the Vibrio vulnificus vvpE expression by cyclic AMP-receptor protein and quorum-sensing regulator SmcR.
In Vibrio vulnificus, cAMP-receptor protein (CRP) and the quorum-sensing regulator SmcR are simultaneously and cooperatively required for the metalloprotease vvpE gene expression, rather than sequentially in a regulatory cascade. However, this study shows a new temporal and functional sequence between the two factors in regulating vvpE expression. A crp mutation inhibited vvpE expression with growth impairment from early stage. In contrast, a smcR mutation inhibited vvpE expression only at the late stage with no effect on growth. A crp-smcR double mutation severely inhibited vvpE expression with growth impairment from early stage. The inhibited vvpE expression was restored only at the early stage by a crp single complementation, but not at all by a smcR complementation. These results indicate that CRP functions as an essential activator, whereas SmcR functions in the presence of CRP for full vvpE expression.
Kim
Choon-Mee
CM
Research Center for Resistant Cells, Chosun University Medical School, 375 Seosuk-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
Shin
Sung-Heui
SH
D001426:Bacterial Proteins / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D045726:Metalloproteases / Q000096:biosynthesis*; D053038:Quorum Sensing; D017384:Sequence Deletion; D015534:Trans-Activators / Q000378:metabolism*; D041261:Vibrio vulnificus / Q000502:physiology*
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D015534:Trans-Activators; C410093:smcR protein, Vibrio vulnificus; D045726:Metalloproteases