title: Cyclosporine-impregnated allograft bone sterilized with low-temperature plasma.
abstract: Deep-freezing, freeze-drying and gamma (γ)-irradiation have deleterious effects on bone healing and mechanical properties of allograft bones. We tried preparing bone allografts using cyclosporine plus low-temperature-plasma sterilization. To explore the feasibility of this method of preparation, segmental defects in the right radii of rabbits were repaired with cyclosporine-impregnated allograft bones (CABs) sterilized with low-temperature-plasma (in the study group) and deep-frozen/freeze-dried irradiated allograft bones (D/FIABs) (in the control group). X-ray and quantitative histological analysis, peripheral blood T lymphocyte subset analysis and CD₂₅ molecule immunohistochemistry stain, the four-point bending test and safety evaluations were respectively conducted to compare bone-healing, immunosuppression, mechanical properties and safety between the two groups. X-ray scores were higher in the study group than those in the control (p = 0.032). There were significant differences in new bone areas at most repairs in both groups (p < 0.05). There were no significant differences in the percentages of CD₄(+) T, CD₈(+) T, ratios of CD₄(+) T:CD₈(+) T or serum concentrations of GPT/Cr in both groups (p > 0.05). At 16 weeks postoperatively, the density of CD₂₅ molecules in the control group was higher than that in the study group. The ultimate loading in the study group was significantly higher than that in the control (p = 0.048). Bone marrow stromal cells (BMSCs) grew thickly around and on the surface of a cyclosporine-impregnated allograft. Livers and kidneys in the study and control groups remained histologically normal at 7 days postoperatively. These results indicate that the CAB might be a better material than the D/FIAB in terms of bone healing, preservation of mechanical properties and immunosuppression without severe side-effects.
authors.affiliation: Institute of Orthopedics of Chinese PLA, Chinese PLA General Hospital, Beijing 100853, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Lu
authors.forename: Guoxian
authors.initials: G
authors.lastname: Pei
authors.forename: Peiran
authors.initials: P
authors.lastname: Zhao
authors.forename: Shuangwu
authors.initials: S
authors.lastname: Liang
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D016025:Bone Transplantation; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D007150:Immunohistochemistry; D020007:Implants, Experimental; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D058626:Plasma Gases / Q000494:pharmacology*; D011817:Rabbits; D011859:Radiography; D013242:Sterilization; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013696:Temperature; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000187:drug effects
chemical_list: D058626:Plasma Gases; D016572:Cyclosporine