title: Analysis of rhesus rhadinovirus microRNAs expressed in virus-induced tumors from infected rhesus macaques.
abstract: Rhesus rhadinovirus (RRV), a primate gamma-herpesvirus related to human Kaposi's sarcoma-associated herpesvirus (KSHV), causes a similar pattern of pathogenesis. Previously, RRV was shown to express 7 pre-microRNAs (pre-miRNAs) in latently infected cells. Using deep sequencing, we analyzed the pattern of small RNA expression in vivo using latently RRV-infected B-cell lymphoma and retroperitoneal fibromatosis tissues. We identified 15 virally encoded pre-miRNAs in both tumors, including all previously reported RRV pre-miRNAs. Although all 15 RRV pre-miRNAs, like all 12 KSHV pre-miRNAs, are located 3' to the conserved viral ORF71 gene and in the same transcriptional orientation, only one RRV miRNA is homologous to a KSHV miRNA. One previously identified RRV miRNA, miR-rR1-3, is actually a miRNA offset RNA (moRNA) derived from sequences located adjacent to pre-miR-rR1-3. Several other RRV-derived moRNAs were obtained, including one recovered >600 times. Together, this research provides a comprehensive list of the miRNAs and moRNAs encoded by RRV.
authors.affiliation: Department of Molecular Genetics & Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Umbach
authors.forename: Lisa I
authors.initials: LI
authors.lastname: Strelow
authors.forename: Scott W
authors.initials: SW
authors.lastname: Wong
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Cullen
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005350:Fibroma / Q000821:virology*; D006566:Herpesviridae Infections / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D016393:Lymphoma, B-Cell / Q000821:virology*; D008253:Macaca mulatta; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012186:Retroperitoneal Neoplasms / Q000821:virology*; D018144:Rhadinovirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D014412:Tumor Virus Infections / Q000821:virology; D017735:Virus Latency
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral