Abnormal immunoexpression of cell adhesion molecules (CAMs) in cervical cancer.
The purpose of this study was to examine the immunoexpression of cell adhesion molecules (CAMs) E-cadherin, CD44s, and CD44v3 in cervical cancer and compare it with that in benign exo-endocervical tissue. In all, 81 cervical cancer biopsy specimens and 22 benign controls were included. Primary monoclonal antibodies NHC-38, F10-44-2, and 3G5 for E-cadherin, CD44s, and CD44v3 were used, respectively. Statistical significance was evaluated by the χ(2) test. Antigen expression was significantly different in cervical cancer specimens compared with controls, showing marked decrease in membrane expression: E-cadherin, 6.5% and 77.3% (P < .000); CD44s, 3.9% and 81.8% (P < .000); and CD44v3, 0% and 81.8% (P < .000), respectively. The immunoexpression was significantly heterogeneous in carcinomas (P < .034) and adenocarcinomas (P < .000) for E-cadherin and CD44s. For CD44v3, no case of cancer showed immunostaining in membranes. These findings reaffirm that cell adhesion is markedly altered in cervical cancer. The authors suggest that these proteins could serve as markers for invasive cervical neoplasia.
de Méndez
Morelva Toro
MT
School of Pharmaceutics and Analytical Biosciences, University of Los Andes, Mérida, Venezuela. tmorelva@ula.ve
Bosch
Antonio Llombart
AL
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D015344:DNA Probes, HPV; D004279:DNA, Viral; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D046888:Tissue Array Analysis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology
D014408:Biomarkers, Tumor; C473647:CD44S antigen; C121450:CD44V3,8-10; D015820:Cadherins; D015344:DNA Probes, HPV; D004279:DNA, Viral; D018960:Hyaluronan Receptors