title: Protective effects of black cumin (Nigella sativa) oil on TNBS-induced experimental colitis in rats.
abstract: BACKGROUND
  The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis.
  
  
  METHODS
  Experimental colitis was induced with 1 mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intracolonic administration with 8-cm-long cannula under ether anesthesia to rats in colitis group and colitis + black cumin oil group. Rats in the control group were given saline at the same volume by intracolonic administration. Black cumin oil (BCO, Origo "100% natural Black Cumin Seed Oil," Turkey) was given to colitis + black cumin oil group by oral administration during 3 days, 5 min after colitis induction. Saline was given to control and colitis groups at the same volume by oral administration. At the end of the experiment, macroscopic lesions were scored and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde, and glutathione levels, collagen content, and tissue factor, superoxide dismutase, and myeloperoxidase activities. Tissues were also examined by histological and cytological analysis. Proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6], lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples.
  
  
  RESULTS
  We found that black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis.
  
  
  CONCLUSIONS
  BCO, by preventing inflammatory status in the blood, partly protected colonic tissue against experimental ulcerative colitis.
authors.affiliation: Faculty of Dentistry, Department of Biochemistry, Marmara University, Nisantası, 34365, Istanbul, Turkey. ferda_isik@hotmail.com
authors.forename: F
authors.initials: F
authors.lastname: Isik
authors.forename: Tugba
authors.initials: T
authors.lastname: Tunali Akbay
authors.forename: A
authors.initials: A
authors.lastname: Yarat
authors.forename: Z
authors.initials: Z
authors.lastname: Genc
authors.forename: R
authors.initials: R
authors.lastname: Pisiriciler
authors.forename: E
authors.initials: E
authors.lastname: Caliskan-Ak
authors.forename: S
authors.initials: S
authors.lastname: Cetinel
authors.forename: A
authors.initials: A
authors.lastname: Altıntas
authors.forename: G
authors.initials: G
authors.lastname: Sener
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D031881:Nigella sativa; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity; D029974:Carum
chemical_list: D016207:Cytokines; D010938:Plant Oils; D014280:Triglycerides; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid; D002784:Cholesterol; C104525:caraway oil; D007770:L-Lactate Dehydrogenase