A new antioxidant compound from Capparis spinosa.
An activity-directed fractionation and purification process was used to isolate 1,1-diphenyl-2-picrylhydrazyl radical (DPPH*) scavenging components from fruits of Capparis spinosa L. (Capparaeae). Ethyl acetate and aqueous fractions showed greater DPPH* scavenging activities compared to the petroleum ether fractions. The ethyl acetate fraction was subjected to purification using column chromatography. A new antioxidant cappariside (4-hydroxy-5-methylfuran-3-carboxylic acid, 1), together with seven known organic acids (2-8) for the first time from plants of genus Capparis and four known organic acids (9-12) were isolated from C. spinosa. The structures were elucidated by extensive analysis of 1D- and 2D-NMR spectroscopic. In addition, compounds 1, 2, 4, 5, 9, 10 and 12 indicated strong scavenging capacity for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with a SC(50) value of 0.204 +/- 0.002, 0.007 +/- 0.0, 0.011 +/- 0.0, 0.044 +/- 0.0016, 0.032 +/- 0.0, 0.090 +/- 0.001, and 0.350 +/- 0.017 mM, respectively.
Yang
Tao
T
Key Laboratory of Standardization of Chinese Medicines of the Ministry of Education, Shanghai, PR China.
Wang
Changhong
C
Liu
Hongjuan
H
Chou
Guixin
G
Cheng
Xuemei
X
Wang
Zhengtao
Z
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D031484:Capparis; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts