Proinflammatory mediators in nasal lavage of subjects with occupational rhinitis.
We sought to investigate the type and kinetics of late-phase nasal inflammatory response after nasal challenge with occupational allergens. Participants were 10 subjects experiencing work-related rhinitis symptoms who underwent specific inhalation challenge and tested positive for occupational rhinitis. During challenge, we monitored changes in inflammatory cells, eosinophil cationic protein, myeloperoxidase, and interleukin-8 in nasal lavage samples. The challenge with the active agent induced a significant increase in the percentage of eosinophils at 30 minutes as compared with prechallenge values (P = 0.04). A significant increase in eosinophil cationic protein levels after challenge with the control (P = 0.01) and active agent (P = 0.02) was observed in the late phase after challenge. No significant changes in nasal levels of neutrophils, myeloperoxidase, and interleukin-8 were observed on both control and active challenge days. Our results suggest a predominant nasal eosinophilic inflammatory response after occupational allergen challenge.
Castano
Roberto
R
Hôpital du Sacré-Coeur de Montréal, Montréal, Quebec, Canada. roberto.castano@mail.mcgill.ca
Maghni
Karim
K
Castellanos
Lucero
L
Trudeau
Carole
C
Malo
Jean-Luc
JL
Gautrin
Denyse
D
D000280:Administration, Inhalation; D000704:Analysis of Variance; D004797:Enzyme-Linked Immunosorbent Assay; D047150:Eosinophil Cationic Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D007785:Lactose / Q000008:administration & dosage; D008297:Male; D009504:Neutrophils; D016273:Occupational Exposure / Q000009:adverse effects*; D009195:Peroxidase / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000378:metabolism*; D012882:Skin Tests; D018709:Statistics, Nonparametric; D007507:Therapeutic Irrigation
D018836:Inflammation Mediators; D016209:Interleukin-8; D009195:Peroxidase; D047150:Eosinophil Cationic Protein; D007785:Lactose