title: Climate chamber for environmentally controlled laboratory airflow experiments.
abstract: Climate chambers have been widely used in in vitro and in vivo studies which require controlled environmental temperature and humidity conditions. This article describes a new desktop climate chamber that was developed for application of respiratory airflows on cultured nasal epithelial cells (NEC) under controlled temperature and humidity conditions. Flow experiments were performed by connecting the climate chamber to an airflow generator via a flow chamber with cultured NEC. Experiments at two controlled climate conditions, 25 degrees C and 40% relative humidity (RH) and 37 degrees C and 80%RH, were conducted to study mucin secretion from the cultures inresponse to the flow. The new climate chamber is a relatively simple and inexpensive apparatus which can easily be connected to any flow system for climate controlled flow experiments. This chamber can be easily adjusted to various in vitro experiments, as well as to clinical studies with animals or human subjects which require controlled climate conditions.
authors.affiliation: Department of Biomedical Engineering, Tel Aviv University, Tel Aviv, Israel. nuritet@eng.tau.ac.il
authors.forename: Nurit
authors.initials: N
authors.lastname: Even-Tzur
authors.forename: Uri
authors.initials: U
authors.lastname: Zaretsky
authors.forename: Orly
authors.initials: O
authors.lastname: Grinberg
authors.forename: Tomer
authors.initials: T
authors.lastname: Davidovich
authors.forename: Yoel
authors.initials: Y
authors.lastname: Kloog
authors.forename: Michael
authors.initials: M
authors.lastname: Wolf
authors.forename: David
authors.initials: D
authors.lastname: Elad
mesh_terms: D000392:Air Movements; D002478:Cells, Cultured; D004780:Environment, Controlled; D006801:Humans; D006813:Humidity; D009297:Nasal Mucosa; D012106:Research / Q000295:instrumentation*; D013696:Temperature