[Integrin-associated proteins in blood plasma of patients with acute exacerbation of chronic obstructive pulmonary disease].
OBJECTIVE
To explore the role of CD(47) and P-selectin in patients with COPD.


METHODS
The blood plasma levels of CD(47) and P-selectin in 20 healthy volunteers (control group) and 35 patients with COPD in acute exacerbation and at stable stage were measured by flow cytometry. Platelet count was measured by hematology analyzer. SNK-q test and Pearson correlation analysis of the regression equation were used for statistics.


RESULTS
The level of CD(47) in the COPD acute exacerbation group [(93 +/- 4)%] was significantly higher than that of the COPD stable group [(72 +/- 11)%] and that of the control group [(67 +/- 10)%], q = 11.26, 13.32, all P < 0.01, but the difference in CD(47) between the stable group and the control group was not significant (q = 1.73, P > 0.05). The level of P-selectin in the acute exacerbation group [(35 +/- 11)%] was significantly higher than those of the stable group [(12 +/- 8)%] and the control group [(10 +/- 4)%] (q = 9.93, 12.19, all P < 0.05), and the level of stable group was also higher than that of the control group (q = 1.90, P < 0.05). The difference in platelet counts among the acute exacerbation group [(188 +/- 56) x 10(9)/L], the stable group [(213 +/- 57) x 10(9)/L] and the control group [(204 +/- 51) x 10(9)/L] was not significant (F = 1.74, P > 0.05). A significant positive correlation between CD(47) and P-selectin was observed in the acute exacerbation group(r = 0.77, P < 0.01), but not in the stable group and the control group (r = -0.04, -0.15, all P > 0.05).


CONCLUSIONS
The levels of CD(47) and P-selectin as markers of platelet activation increase significantly during AECOPD, which suggests that platelet activation exists in AECOPD and platelets as a type of inflammatory cells participate in the disease.
Pan
Lei
L
Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Yang
Yu-hao
YH
Dong
De-qiong
DQ
D000368:Aged; D000369:Aged, 80 and over; D051928:CD47 Antigen / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000503:physiopathology
D051928:CD47 Antigen; D019007:P-Selectin