title: A eubacterial riboswitch class that senses the coenzyme tetrahydrofolate.
abstract: Comparative sequence analyses of bacterial genomes are revealing many structured RNA motifs that function as metabolite-binding riboswitches. We have identified an RNA motif frequently positioned in the 5' UTRs of folate transport and biosynthesis genes in Firmicute genomes. Biochemical experiments confirm that representatives of this new-found RNA class selectively bind derivatives of the vitamin folate, including di- and tetrahydrofolate coenzymes. In addition, representatives of this aptamer class occasionally reside upstream of RNA structures that are predicted to control translation initiation in response to ligand binding. These findings expand the number of coenzymes that are directly sensed by RNA and reveal possible riboswitch-controlled regulons that respond to changes in single-carbon metabolism.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, Box 208103, New Haven, CT 06520-8103, USA.
authors.forename: Tyler D
authors.initials: TD
authors.lastname: Ames
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Rodionov
authors.forename: Zasha
authors.initials: Z
authors.lastname: Weinberg
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Breaker
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism*; D019295:Computational Biology; D017124:Conserved Sequence; D005051:Eubacterium / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038621:Regulatory Sequences, Ribonucleic Acid; D013763:Tetrahydrofolates / Q000378:metabolism*
chemical_list: D027321:Anion Transport Proteins; D052157:Aptamers, Nucleotide; D003067:Coenzymes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid; D013763:Tetrahydrofolates; C030371:5,6,7,8-tetrahydrofolic acid; D002244:Carbon