title: Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma.
abstract: Cutaneous squamous cell carcinoma (cSCC) results from transformation of epidermal keratinocytes. Invasion of transformed keratinocytes through the basement membrane into the dermis results in invasive cSCC with substantial metastatic potential. To better understand the mechanisms for invasion and metastasis, we compared the protein expression profiles of a non-metastatic transformed mouse keratinocyte line and its metastatic derivative. Keratin 8 (Krt8) and Krt18, not seen in normal keratinocytes, were coexpressed and formed Krt8/18 filaments in the metastatic line. The metastatic line efficiently invaded an artificial basement membrane in vitro owing to the Krt8/18-coexpression, since coexpression of exogenous Krt8/18 in the non-invasive parental line conferred invasiveness. To test whether the Krt8/18-coexpression is induced and is involved in cSCC invasion, we examined specimens from 21 pre-invasive and 24 invasive cSCC patients by immunohistochemistry, and the ectopic Krt8/18-coexpression was almost exclusively found in invasive cSCC. Further studies are needed to examine the clinical significance of ectopic Krt8/18-coexpression in cSCC.
authors.affiliation: Department of Medical Biochemistry, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Yamashiro
authors.forename: Kimiko
authors.initials: K
authors.lastname: Takei
authors.forename: Masato
authors.initials: M
authors.lastname: Umikawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Asato
authors.forename: Minoru
authors.initials: M
authors.lastname: Oshiro
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Uechi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Taira
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uezato
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kariya
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D053538:Keratin-18 / Q000096:biosynthesis*; D053533:Keratin-8 / Q000096:biosynthesis*; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D012878:Skin Neoplasms / Q000473:pathology*
chemical_list: D053538:Keratin-18; D053533:Keratin-8