Breed distribution of the nt230(del4) MDR1 mutation in dogs.
A 4-bp deletion mutation associated with multiple drug sensitivity exists in the canine multidrug resistance (MDR1) gene. This mutation has been detected in more than 10 purebred dog breeds as well as in mixed breed dogs. To evaluate the breed distribution of this mutation in Germany, 7378 dogs were screened, including 6999 purebred and 379 mixed breed dogs. The study included dog breeds that show close genetic relationship or share breeding history with one of the predisposed breeds but in which the occurrence of the MDR1 mutation has not been reported. The breeds comprised Bearded Collies, Anatolian Shepherd Dog, Greyhound, Belgian Tervuren, Kelpie, Borzoi, Australian Cattle Dog and the Irish Wolfhound. The MDR1 mutation was not detected is any of these breeds, although it was found as expected in the Collie, Longhaired Whippet, Shetland Sheepdog, Miniature Australian Shepherd, Australian Shepherd, Wäller, White Swiss Shepherd, Old English Sheepdog and Border Collie with varying allelic frequencies for the mutant MDR1 allele of 59%, 45%, 30%, 24%, 22%, 17%, 14%, 4% and 1%, respectively. Allelic frequencies of 8% and 2% were determined in herding breed mixes and unclassified mixed breeds, respectively. Because of its widespread breed distribution and occurrence in many mixed breed dogs, it is difficult for veterinarians and dog owners to recognise whether MDR1-related drug sensitivity is relevant for an individual animal. This study provides a comprehensive overview of all affected dog breeds and many dog breeds that are probably unaffected on the basis of ∼15,000 worldwide MDR1 genotyping data.
Gramer
Irina
I
Institute of Pharmacology and Toxicology, Justus Liebig University of Giessen, Frankfurter Str. 107, D-35392 Giessen, Germany.
Leidolf
Regina
R
Döring
Barbara
B
Klintzsch
Stefanie
S
Krämer
Eva-Maria
EM
Yalcin
Ebru
E
Petzinger
Ernst
E
Geyer
Joachim
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000818:Animals; D004285:Dogs / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D005787:Gene Frequency; D019450:Genes, MDR / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D005858:Germany; D009154:Mutation; D013045:Species Specificity
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1