Combination of conjugated linoleic acid with fish oil prevents age-associated bone marrow adiposity in C57Bl/6J mice.
The inverse relationship between fat in bone marrow and bone mass in the skeleton of aging subjects is well known. However, there is no precise therapy for the treatment of bone marrow adiposity. We investigated the ability of conjugated linoleic acid (CLA) and fish oil (FO), alone or in combination, to modulate bone loss using 12 months old C57Bl/6J mice fed 10% corn oil diet as control or supplemented with 0.5% CLA or 5% FO or 0.5% CLA+5% FO for 6 months. We found, CLA-fed mice exhibited reduced body weight, body fat mass (BFM) and enhanced hind leg lean mass (HLLM) and bone mineral density (BMD) in different regions measured by dual energy x-ray absorptiometry (DXA); however, associated with fatty liver and increased insulin resistance; whereas, FO fed mice exhibited enhanced BMD, improved insulin sensitivity, with no changes in BFM and HLLM. Interestingly, CLA+FO fed mice exhibited reduced body weight, BFM, peroxisome proliferator-activated receptor gamma and cathepsin K expression in bone marrow with enhanced BMD and HLLM. Moreover, CLA+FO supplementation reduced liver hypertrophy and improved insulin sensitivity with remarkable attenuation of bone marrow adiposity, inflammation and oxidative stress in aging mice. Therefore, CLA with FO combination might be a novel dietary supplement to reduce fat mass and improve BMD.
Halade
Ganesh V
GV
Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
Rahman
Md M
MM
Williams
Paul J
PJ
Fernandes
Gabriel
G
D015502:Absorptiometry, Photon / Q000379:methods; D000273:Adipose Tissue / Q000187:drug effects*; D050154:Adiposity / Q000187:drug effects*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001823:Body Composition; D001835:Body Weight; D015519:Bone Density; D001853:Bone Marrow / Q000378:metabolism; D003314:Corn Oil / Q000008:administration & dosage; D004032:Diet; D019587:Dietary Supplements; D004359:Drug Therapy, Combination; D005234:Fatty Liver / Q000378:metabolism / Q000517:prevention & control; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D007333:Insulin Resistance; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D010024:Osteoporosis / Q000378:metabolism / Q000517:prevention & control; D047495:PPAR gamma / Q000378:metabolism
D005395:Fish Oils; D044243:Linoleic Acids, Conjugated; D047495:PPAR gamma; D003314:Corn Oil