Estrogenic activity of a naringinase-treated extract of Sophora japonica cultivated in Egypt.
The naringinase-treated methanol extract of Sophora japonica L. (Fabaceae) seeds showed potent estrogen agonist activity. Through bioassay-guided isolation of the main active constituents from the naringinase-treated methanol extract of S. japonica, the aglycones genistein and kaempferol were found to be the main phytoestrogens in the naringinase-treated extract. In addition, kaempferol was nearly equipotent to genistein as an estrogen agonist. Concerning the compounds isolated from the untreated methanol extract, sophoricoside showed weak estrogenic activity on ERbeta only.
El-Halawany
Ali M
AM
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Chung
Mi Hwa
MH
Abdallah
Hossam M
HM
Nishihara
Tsutomu
T
Hattori
Masao
M
D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004063:Digestion; D055808:Drug Discovery; D004534:Egypt; D004965:Estrogen Antagonists / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000235:genetics; D047629:Estrogen Receptor beta / Q000819:agonists / Q000235:genetics; D019833:Genistein / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000378:metabolism*; D009994:Osmolar Concentration; D048789:Phytoestrogens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D029909:Sophora / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D001617:beta-Glucosidase / Q000378:metabolism*
D001578:Benzopyrans; D004965:Estrogen Antagonists; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D044949:Kaempferols; D009097:Multienzyme Complexes; D048789:Phytoestrogens; D010936:Plant Extracts; C120642:sophoricoside; C006552:kaempferol; D019833:Genistein; C039827:naringinase; D001617:beta-Glucosidase