Correlation of TLR1-10 expression in peripheral blood mononuclear cells with chronic hepatitis B and chronic hepatitis B-related liver failure.
Toll-like receptors (TLRs) promote host defenses against invading viruses and pathogenic bacteria through corresponding adapter molecules leading to the initiation of innate immune response. We investigated the expression of TLR1-10 in peripheral blood mononuclear cells (PBMCs) from patients with different clinical phases of chronic hepatitis B (CHB) infection and analyzed the correlation between TLRs and clinical profiles. Our results showed that expression of TLR3/5/7/9/10 and TLR2/4/6 mRNA was upregulated in active stage of CHB and CHB-related liver failure, respectively. Particularly we found that TLR9 mRNA expression is negatively correlated with serum alanine aminotransferase (ALT), Tbil, PTA, and positively correlated with hepatitis B virus (HBV) viral load in CHB patients. Our results indicate that innate immune responses are significantly higher in CHB active phase than that in CHB-related liver failure, suggesting TLRs may play a critical role in development of CHB and CHB-related liver failure, the mechanisms need further to be further explored.
Wang
Ke
K
Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Shaanxi Province, China.
Liu
Hongli
H
He
Yingli
Y
Chen
Tianyan
T
Yang
Yuan
Y
Niu
Yinghua
Y
Chen
Hongmei
H
Chen
Yunru
Y
Liu
Jinfeng
J
Ye
Feng
F
Lin
Shumei
S
Zhao
Yingren
Y
D000328:Adult; D000410:Alanine Transaminase / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D006515:Hepatitis B virus / Q000472:pathogenicity / Q000502:physiology*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D007113:Immunity, Innate; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D017093:Liver Failure; D008297:Male; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019562:Viral Load / Q000276:immunology
D051193:Toll-Like Receptors; D000410:Alanine Transaminase