title: Fibers with polypyrrole and polythiophene phases for isolation and determination of adrenolytic drugs from human plasma by SPME-HPLC.
abstract: In this study, polypyrrole (PPy) and polythiophene (PTh) SPME coatings and their ability to extract selected adrenolytic drugs with different physico-chemical properties from standard solutions and human plasma samples were evaluated. In measurements metoprolol, oxprenolol, mexiletine, propranolol, and propaphenon were investigated. The main parameters such as extraction time, desorption conditions and pH influence were examined. Inter-day precisions were in range 0.1-2.0%, 1.1-2.9%, 1.3-2.6%, 0.1-2.6% and 0.3-2.1% for metoprolol, oxprenolol, mexiletine, propranolol and propaphenon, respectively. Accuracies were less than 15%, which was evaluated by analyzing preparation samples of five replicates. The method was successfully applied to human plasma samples spiked with selected adrenolytic drugs. The method was linear in the concentration range from 1 to 10microg/ml for all of studied adrenolytic drugs using human plasma samples. The PTh-SPME coating displayed higher extraction efficiency towards the target analytes in comparison to PPy-SPME. The reproducibility of the extraction using polypyrrole and polythiophene fibers was confirmed by variation coefficients lower than 8% and 3%, respectively.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7 Street, Torun, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Olszowy
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szultka
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ligor
authors.forename: Jacek
authors.initials: J
authors.lastname: Nowaczyk
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D018674:Adrenergic Antagonists / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D011108:Polymers / Q000737:chemistry*; D011412:Propanolamines / Q000097:blood; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D018674:Adrenergic Antagonists; D011108:Polymers; D011412:Propanolamines; D011758:Pyrroles; D013876:Thiophenes; C066730:polythiophene; C067635:polypyrrole