Circulating and local visfatin/Nampt/PBEF levels in spontaneously hypertensive rats, stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats.
Visfatin (also known as nicotinamide phosphoribosyltransferase and pre-B cell colony-enhancing factor) is a multifunctional protein. Visfatin has been reported to be involved in several biological processes in the cardiovascular system, . However, the role of visfatin in hypertension is still unclear. In this study, we examined the circulating and local adipose visfatin levels in spontaneously hypertensive rats (SHR), stroke-prone spontaneously hypertensive rats (SHR-SP), and in their normotensive control Wistar-Kyoto (WKY). SHR and SHR-SP rats exhibited lower body weight, lower fat tissue and hypolipidemia. No differences of serum visfatin levels were observed in SHR/SHR-SP and WKY. Serum visfatin levels did not correlate to serum glucose, lipids, insulin, and fat pad weights, but significantly correlated to weights of skeletal muscle. Visfatin expression in visceral fat tissue was slightly lower in SHR-SP compared with that in WKY. Moreover, there were no significant differences of visfatin expression in skeletal muscles among WKY, SHR and SHR-SP. Finally, visfatin protein was detected in L6 rat skeletal muscle cell culture medium, indicating that visfatin was secreted from skeletal muscle cells. Thus, our results may provide useful information for understanding the characteristic of visfatin in hypertensive models, and support the view that visfatin may be a myokine.
Wang
Pei
P
Department of Pharmacology, Second Military Medical University, 325 Guo He Road, Shanghai, 200433, China.
Du
Hui
H
Zhang
Ruo-Yu
RY
Guan
Yun-Feng
YF
Xu
Tian-Ying
TY
Xu
Quan-Yi
QY
Su
Ding-Feng
DF
Miao
Chao-Yu
CY
D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D001835:Body Weight; D006339:Heart Rate; D006973:Hypertension / Q000097:blood* / Q000503:physiopathology; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020521:Stroke / Q000097:blood* / Q000503:physiopathology
D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D054409:Nicotinamide Phosphoribosyltransferase