Serinicoccus chungangensis sp. nov., isolated from tidal flat sediment, and emended description of the genus Serinicoccus.
A Gram-positive, halophilic bacterium, strain CAU 9536(T), was isolated from a tidal flat sediment in the Yellow Sea, Republic of Korea. Strain CAU 9536(T) grew optimally at 30 °C, at pH 9.0 and in the presence of 13.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 9536(T) belonged to the genus Serinicoccus, and showed sequence similarity levels of 99.0 % to Serinicoccus profundi MCCC 1A05965(T) and 98.0 % to Serinicoccus marinus JC1078(T). DNA-DNA relatedness values between strain CAU 9536(T) and the above two type strains were below 45.0 %. The cell-wall peptidoglycan of strain CAU 9536(T) was based on meso-diaminopimelic acid, in contrast to those of S. profundi and S. marinus. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. Whole-cell hydrolysates contained mainly glucose and ribose. The major isoprenoid quinone was menaquinone MK-8 (H(4)), and the predominant cellular fatty acid was iso-C(15 : 0). The DNA G+C content of strain CAU 9536(T) was 73.5 mol%. Based on phenotypic, chemotaxonomic and genotypic data, strain CAU 9536(T) is considered to represent a novel species of the genus Serinicoccus, for which the name Serinicoccus chungangensis sp. nov. is proposed. The type strain is CAU 9536(T) ( = KCTC 19774(T) = CCUG 59777(T)).
Traiwan
Jitsopin
J
Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Park
Mi-Hak
MH
Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Kim
Wonyong
W
Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid