title: In vitro studies of interaction of modified silica nanoparticles with different types of immunocompetent cells.
abstract: Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol-gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence-bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated.
authors.affiliation: Institution of Russian Academy of Sciences Institute of Solution Chemistry of the RAS, Ivanovo 153045, Russian Federation.
authors.forename: Galina A
authors.initials: GA
authors.lastname: Kulikova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Parfenyuk
authors.forename: Irina V
authors.initials: IV
authors.lastname: Ryabinina
authors.forename: Yuliya S
authors.initials: YS
authors.lastname: Antsiferova
authors.forename: Nataliya Yu
authors.initials: NY
authors.lastname: Sotnikova
authors.forename: Lubov V
authors.initials: LV
authors.lastname: Posiseeva
authors.forename: Mariya A
authors.initials: MA
authors.lastname: Eliseeva
mesh_terms: D000327:Adsorption; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism*; D008422:Materials Testing; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012756:Sheep; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate