Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis via a caspase-dependent pathway as compared to Ophiopogon japonicus lectin.
Galanthus nivalis agglutinin (GNA)-related lectin family, a superfamily of strictly mannose-binding specific lectins, has been well-known to possess several biological functions including apoptosis-inducing activities. However, the precise mechanisms of GNA-related lectins to induce apoptosis remains to be clarified. In this study, we showed that Polygonatum cyrtonema lectin (PCL) and Ophiopogon japonicus lectin (OJL), the two mannose-binding GNA-related lectins, could induce murine fibrosarcoma L929 cell apoptosis. In addition, we found that there was a close link between their sugar-binding and apoptosis-inducing activities. Interestingly, we further confirmed that the mechanism of lectin-induced apoptosis was a caspase-dependent pathway. Moreover, we found that the two lectins could amplify tumor necrosis factor α (TNFα)-induced apoptosis. Taken together, these findings would open a new perspective for GNA-related lectins as potential anti-tumor agents.
Zhang
Zi-ting
ZT
School of Life Sciences, State Key Laboratory of Oral Diseases, Sichuan University, Wuhou District, Chengdu, China.
Peng
Hao
H
Li
Chun-yang
CY
Liu
Jun-jie
JJ
Zhou
Ting-Ting
TT
Yan
Yi-fang
YF
Li
Yan
Y
Bao
Jin-ku
JK
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D031441:Ophiopogon / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D031384:Polygonatum / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D020169:Caspases