title: Phenylalanine hydroxylase expression in primary rat hepatocytes is modulated by oxygen concentration.
abstract: In this work we have investigated the regulation of rat phenylalanine hydroxylase (rPAH) expression by oxygen in primary cultures of rat hepatocytes. We show that rPAH is negatively modulated at the mRNA, protein and activity levels by pO(2) of 16% (periportal hepatic levels) compared to 8% (perivenous hepatic levels). Our results suggest that PAH might be metabolically zonated in vivo, and preferentially found in perivenous hepatocytes with high glucose consumption and largely influenced by insulin levels.
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway.
authors.forename: Ming
authors.initials: M
authors.lastname: Ying
authors.forename: Angel L
authors.initials: AL
authors.lastname: Pey
authors.forename: Niels
authors.initials: N
authors.lastname: Aarsaether
authors.forename: Aurora
authors.initials: A
authors.lastname: Martinez
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D022781:Hepatocytes / Q000201:enzymology*; D008297:Male; D010100:Oxygen / Q000502:physiology*; D010651:Phenylalanine Hydroxylase / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010651:Phenylalanine Hydroxylase; D010100:Oxygen