The polycotyledon (pct1-2) mutant of tomato shows enhanced accumulation of PIN1 auxin transport facilitator protein.
The bilateral symmetry of a dicotyledon embryo is tightly associated with the directional flow of auxin. Disruption of polar auxin flow results in various developmental abnormalities. The pct1-2 mutant of tomato, showing polycotyledony, also has enhanced polar auxin transport in hypocotyls. Immunocytochemical analysis revealed increased PIN1 protein in pct1-2 roots and hypocotyls. The mutant also displayed an increase in PIN1 transcript levels in these organs. Our results indicate that over-accumulation of PIN1 protein is likely related to increased polar transport of auxin in the pct1-2 mutant.
Kharshiing
E V
EV
School of Life Sciences, University of Hyderabad, Hyderabad, India.
Kumar
G P
GP
Ditengou
F A
FA
Li
X
X
Palme
K
K
Sharma
R
R
D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D021381:Protein Transport
D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010940:Plant Proteins