Dynamics of the coiled-coil unfolding transition of myosin rod probed by dissipation force spectrum.
The motor protein myosin II plays a crucial role in muscle contraction. The mechanical properties of its coiled-coil region, the myosin rod, are important for effective force transduction during muscle function. Previous studies have investigated the static elastic response of the myosin rod. However, analogous to the study of macroscopic complex fluids, how myosin will respond to physiological time-dependent loads can only be understood from its viscoelastic response. Here, we apply atomic force microscopy using a magnetically driven oscillating cantilever to measure the dissipative properties of single myosin rods that provide unique dynamical information about the coiled-coil structure as a function of force. We find that the friction constant of the single myosin rod has a highly nontrivial variation with force; in particular, the single-molecule friction constant is reduced dramatically and increases again as it passes through the coiled-uncoiled transition. This is a direct indication of a large free-energy barrier to uncoiling, which may be related to a fine-tuned dynamic mechanosignaling response to large and unexpected physiological loads. Further, from the critical force at which the minimum in friction occurs we determine the asymmetry of the bistable landscape that controls uncoiling of the coiled coil. This work highlights the sensitivity of the dissipative signal in force unfolding to dynamic molecular structure that is hidden to the elastic signal.
Taniguchi
Yukinori
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan.
Khatri
Bhavin S
BS
Brockwell
David J
DJ
Paci
Emanuele
E
Kawakami
Masaru
M
D000818:Animals; D004548:Elasticity; D006801:Humans; D008280:Magnetics; D018625:Microscopy, Atomic Force; D015879:Myosin Subfragments / Q000737:chemistry* / Q000378:metabolism; D011489:Protein Denaturation; D011817:Rabbits; D013816:Thermodynamics; D014783:Viscosity
D015879:Myosin Subfragments