Differential response of the catalase, superoxide dismutase and glycerol-3-phosphate dehydrogenase to different environmental stresses in Debaryomyces nepalensis NCYC 3413.
The effect of salt, pH, and temperature stress on the cellular level of antioxidant enzymes, catalase and superoxide dismutase (SOD) and glycerol-3-phosphate dehydrogenase (G3PDH) was studied in Debaryomyces nepalensis NCYC 3413, a halotolerant yeast. The catalase activity increased in different phases, while SOD and G3PDH activities declined in late stationary phase. A significant increase in SOD activity was observed under different stress as compared to control. Salt and temperature stress enhanced the catalase activity where as it was suppressed by pH stress. G3PDH level increased with salt stress, however, no significant change was observed under pH and temperature stress. The observations recorded in this investigation suggested that D. nepalensis has an efficient protective mechanism of antioxidant enzymes and G3PDH against salt, pH, and temperature stresses.
Kumar
Sawan
S
Applied and Industrial Microbiology Laboratory, Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India.
Kalyanasundaram
Gayathiri T
GT
Gummadi
Sathyanarayana N
SN
D002374:Catalase / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D004718:Saccharomycetales / Q000201:enzymology* / Q000502:physiology*; D012492:Salts / Q000633:toxicity; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism*; D013696:Temperature
D012492:Salts; D005993:Glycerolphosphate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase