title: Dendritic signals command firing dynamics in a mathematical model of cerebellar Purkinje cells.
abstract: Dendrites of cerebellar Purkinje cells (PCs) respond to brief excitations from parallel fibers with lasting plateau depolarizations. It is unknown whether these plateaus are local events that boost the synaptic signals or they propagate to the soma and directly take part in setting the cell firing dynamics. To address this issue, we analyzed a likely mechanism underlying plateaus in three representations of a reconstructed PC with increasing complexity. Analysis in an infinite cable suggests that Ca plateaus triggered by direct excitatory inputs from parallel fibers and their mirror signals, valleys (putatively triggered by the local feed forward inhibitory network), cannot propagate. However, simulations of the model in electrotonic equivalent cables prove that Ca plateaus (resp. valleys) are conducted over the entire cell with velocities typical of passive events once they are triggered by threshold synaptic inputs that turn the membrane current inward (resp. outward) over the whole cell surface. Bifurcation analysis of the model in equivalent cables, and simulations in a fully reconstructed PC both indicate that dendritic Ca plateaus and valleys, respectively, command epochs of firing and silencing of PCs.
authors.affiliation: Université Pierre et Marie Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 7222, Paris, France. stephane.genet@upmc.fr
authors.forename: Stéphane
authors.initials: S
authors.lastname: Genet
authors.forename: Loïc
authors.initials: L
authors.lastname: Sabarly
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guigon
authors.forename: Hugues
authors.initials: H
authors.lastname: Berry
authors.forename: Bruno
authors.initials: B
authors.lastname: Delord
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D020013:Calcium Signaling; D003712:Dendrites / Q000378:metabolism*; D008959:Models, Neurological; D011689:Purkinje Cells / Q000378:metabolism*; D015398:Signal Transduction