miR-29b and miR-125a regulate podoplanin and suppress invasion in glioblastoma.
Glioblastoma is the most frequent and malignant brain tumor, characterized by an elevated capacity for cellular proliferation and invasion. Recently, it was demonstrated that podoplanin membrane sialo-glycoprotein encoded by PDPN gene is over-expressed and related to cellular invasion in astrocytic tumors; however the mechanisms of regulation are still unknown. MicroRNAs are noncoding RNAs that regulate gene expression and several biological processes and diseases, including cancer. Nevertheless, their roles in invasion, proliferation, and apoptosis of glioblastoma are not completely understood. In this study, we focused on miR-29b and miR-125a, which were predicted to regulate PDPN, and demonstrated that these microRNAs directly target the 3' untranslated region of PDPN and inhibit invasion, apoptosis, and proliferation of glioblastomas. Furthermore, we report that miR-29b and miR-125a are downregulated in glioblastomas and also in CD133-positive cells. Taken together, these results suggest that miR-29b and miR-125a represent potential therapeutic targets in glioblastoma.
Cortez
Maria Angelica
MA
Department of Experimental Therapeutics and Center for RNA Interference and Non-coding RNAs, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
Nicoloso
Milena Sabrina
MS
Shimizu
Masayoshi
M
Rossi
Simona
S
Gopisetty
Gopal
G
Molina
Jennifer R
JR
Carlotti
Carlos
C
Tirapelli
Daniela
D
Neder
Luciano
L
Brassesco
Maria Sol
MS
Scrideli
Carlos Alberto
CA
Tone
Luiz Gonzaga
LG
Georgescu
Maria-Magdalena
MM
Zhang
Wei
W
Puduvalli
Vinay
V
Calin
George Adrian
GA
D017209:Apoptosis; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000502:physiology*; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098853:Podoplanin
D008562:Membrane Glycoproteins; D035683:MicroRNAs; C117468:PDPN protein, human; D000098853:Podoplanin