title: Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68.
abstract: Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) and provides a small-animal model with which to study the pathogenesis of gammaherpesvirus (gammaHV) infections. To completely explore the potential of the MHV-68 system for the investigation of gammaHV microRNAs (miRNAs), it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By deep sequencing of small RNAs, we systematically investigated the expression profiles of MHV-68 miRNAs in both lytically and persistently infected cells. In addition to the nine known MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68-infected versus noninfected NIH 3T3 fibroblasts and in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated versus nontreated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH 3T3 cells, indicating a potential role for cellular miRNAs during MHV-68 infection. Our data will aid in the full exploration of the functions of gammaHV miRNAs.
authors.affiliation: Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Jia Yun
authors.initials: JY
authors.lastname: Zhu
authors.forename: Martin
authors.initials: M
authors.lastname: Strehle
authors.forename: Anne
authors.initials: A
authors.lastname: Frohn
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Kai P
authors.initials: KP
authors.lastname: Höfig
authors.forename: Gunter
authors.initials: G
authors.lastname: Meister
authors.forename: Heiko
authors.initials: H
authors.lastname: Adler
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D016679:Genome, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D018144:Rhadinovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D017423:Sequence Analysis, RNA
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D012367:RNA, Viral