title: Oral rinse MMP-8 point-of-care immuno test identifies patients with strong periodontal inflammatory burden.
abstract: OBJECTIVE
  To determine whether oral rinse matrix metalloproteinase (MMP)-8 levels, measured by three different methods, tissue inhibitor of matrix metalloprotease-1 (TIMP-1) levels and elastase activity differentiate subjects with different periodontal condition; and second, to find out if MMP-8 levels were comparable among the methods used.
  
  
  METHODS
  MMP-8 levels were analysed with an immunofluorometric method (IFMA), dentoELISA and commercial ELISA. Also TIMP-1 levels and elastase activity were measured. For statistical analysis 214 study subjects were categorized into four groups, specified by the presence and number of moderate (4-5mm) and deep (≥6mm) periodontal pockets, and bleeding on probing percentage.
  
  
  RESULTS
  MMP-8 levels especially measured by dentoELISA and adjusted to the number of teeth per subject differentiated the study group with strong periodontal inflammatory burden from groups with lower levels. This was also verified with receiver operating characteristic (ROC) analysis. Elastase activity associated with higher IFMA and dentoELISA MMP-8 levels. IFMA MMP-8/TIMP and dentoELISA MMP-8/TIMP-1 tended to be higher with the increasing level of periodontal inflammatory burden. TIMP-1 levels decreased with increasing age.
  
  
  CONCLUSIONS
  Oral rinse MMP-8 together with TIMP-1 analysis may have potential in complementary periodontal diagnostics. dentoELISA can be applied in quantitative oral rinse chair side biomarker diagnostics.
authors.affiliation: Institute of Dentistry, University of Helsinki, Helsinki, Finland.
authors.forename: J M
authors.initials: JM
authors.lastname: Leppilahti
authors.forename: M-M
authors.initials: MM
authors.lastname: Ahonen
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: S
authors.initials: S
authors.lastname: Munjal
authors.forename: L
authors.initials: L
authors.lastname: Netuschil
authors.forename: V-J
authors.initials: VJ
authors.lastname: Uitto
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: P
authors.initials: P
authors.lastname: Mäntylä
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D010514:Periodontal Pocket / Q000201:enzymology* / Q000276:immunology; D010518:Periodontitis / Q000201:enzymology* / Q000276:immunology; D019095:Point-of-Care Systems; D012016:Reference Values; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013048:Specimen Handling / Q000379:methods; D018709:Statistics, Nonparametric; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019715:Tissue Inhibitor of Metalloproteinase-1; D010196:Pancreatic Elastase; D020784:Matrix Metalloproteinase 8