The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger.
L-Xylulose reductase is part of the eukaryotic pathway for l-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity. In this communication we identified the 'TRUE'L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on l-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg.
Mojzita
Dominik
D
VTT Technical Research Centre of Finland, Espoo, Finland.
Vuoristo
Kiira
K
Koivistoinen
Outi M
OM
Penttilä
Merja
M
Richard
Peter
P
D001089:Arabinose / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005800:Genes, Fungal; D007700:Kinetics; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014994:Xylose / Q000378:metabolism
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D014994:Xylose; D001089:Arabinose; D013401:Sugar Alcohol Dehydrogenases; C023300:L-xylulose reductase