title: Activation of nervous system development genes in bone marrow derived mesenchymal stem cells following spaceflight exposure.
abstract: Stalled cell division in precursor bone cells and reduced osteoblast function are considered responsible for the microgravity-induced bone loss observed during spaceflight. However, underlying molecular mechanisms remain unraveled. Having overcome technological difficulties associated with flying cells in a space mission, we present the first report on the behavior of the potentially osteogenic murine bone marrow stromal cells (BMSC) in a 3D culture system, flown inside the KUBIK aboard space mission ISS 12S (Soyuz TMA-8 + Increment 13) from March 30 to April 8, 2006 (experiment "Stroma-2"). Flight 1g control cultures were performed in a centrifuge located within the payload. Ground controls were maintained on Earth in another KUBIK payload and in Petri dishes. Half of the cultures were stimulated with osteo-inductive medium. Differences in total RNA extracted suggested that cell proliferation was inhibited in flight samples. Affymetrix technology revealed that 1,599 genes changed expression after spaceflight exposure. A decreased expression of cell-cycle genes confirmed the inhibition of cell proliferation in space. Unexpectedly, most of the modulated expression was found in genes related to various processes of neural development, neuron morphogenesis, transmission of nerve impulse and synapse, raising the question on the lineage restriction in BMSC.
authors.affiliation: Dipartimento di Oncologia, Biologia e Genetica, Universita' di Genova and Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Monticone
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Natalija
authors.initials: N
authors.lastname: Pujic
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
mesh_terms: D000818:Animals; D001853:Bone Marrow; D018797:Cell Cycle Proteins / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells; D051379:Mice; D009420:Nervous System / Q000254:growth & development*; D009474:Neurons; D012313:RNA / Q000032:analysis; D013026:Space Flight; D013569:Synapses / Q000235:genetics; D009435:Synaptic Transmission / Q000235:genetics; D015533:Transcriptional Activation; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D018797:Cell Cycle Proteins; D012313:RNA