title: Nanoneedle: a multifunctional tool for biological studies in living cells.
abstract: Studying biology in living cells is methodologically challenging but highly beneficial. Recent advances in nanobiotechnology offer exciting new opportunities to address this challenge. The nanoneedle technology, as an emerging technology that uses a cell membrane-penetrating nanoneedle to probe and manipulate biological processes in living cells, is expected to play an important role in this endeavor. Here we review the recent development and future direction of the nanoneedle technology for biological studies in living cells. The nanoneedle technology is shown to be powerful and versatile, and can offer numerous new ways to explore biological processes and biophysical properties of living cells with high spatial and temporal precision potentially reaching molecular resolution.
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, Illinois 61801, USA.
authors.forename: Kyungsuk
authors.initials: K
authors.lastname: Yum
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Min-Feng
authors.initials: MF
authors.lastname: Yu
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes; D006801:Humans; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots
chemical_list: D005456:Fluorescent Dyes; D037742:Nanotubes, Carbon