Helicobacter pylori induces expression and secretion of oncostatin M in macrophages in vitro.
BACKGROUND
Helicobacter pylori is pathogenic bacterium that is associated with several gastric diseases in humans. Disease is characterized by severe inflammatory responses is the stomach that are induced by various chemokines and cytokines. Previous reports indicated that some of these responses are mediated through Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling.


METHODS
We performed JAK/STAT specific microarrays to identify new components of this signaling pathway, which are affected by Helicobacter pylori infection of THP-1 cells.


RESULTS
We found that expression and secretion of oncostatin M and of its receptor were strongly up-regulated by Helicobacter pylori. OSM secretion was independent of CagA, VacA or Type IV secretion system. Helicobacter pylori culture supernatant induced OSM secretion.


CONCLUSIONS
The induction of the pleiotropic cytokine oncostatin M suggests a possible role in Helicobacter pylori-mediated inflammation and diseases.
Zeaiter
Zaher
Z
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
Diaz
Hugo
H
Stein
Markus
M
Huynh
Hien Q
HQ
D002460:Cell Line; D016480:Helicobacter pylori; D006801:Humans; D053612:Janus Kinases / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D053683:Oncostatin M / Q000096:biosynthesis / Q000378:metabolism*; D053677:Receptors, Oncostatin M / Q000096:biosynthesis; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation
D053677:Receptors, Oncostatin M; D050791:STAT Transcription Factors; D053683:Oncostatin M; D053612:Janus Kinases