Involvement of selenoprotein P and GPx4 gene expression in cadmium-induced testicular pathophysiology in rat.
To investigate the effect of co-exposure to cadmium (Cd) and selenium (Se) on selenoprotein P (SelP) and phospholipid hydroperoxide glutathione peroxidase (GPx4) gene expression in testis and to evaluate their possible involvement in Cd-induced testicular pathophysiology, male rats received either tap water, Cd or Cd+Se in their drinking water for 5 weeks. Cd exposure caused a down-regulation of SelP and GPx4 gene expression and a significant decrease in plasma and testicular concentrations of Se. These changes were accompanied by decreased plasma testosterone level, sperm count and motility, GSH content, protein-bound sulfhydryl concentration (PSH), enzymatic activities of catalase (CAT) and glutathione peroxidase (GSH-Px) as well as by increased glutathione-S-transferase (GST) activity, lipid peroxidation (as malondialdehyde, MDA) and proteins carbonyls (PC). The decrease of testicular SelP and GPx4 gene expression under Cd influence was significantly restored in Cd+Se group. Co-treatment with Cd and Se also totally reversed the Cd-induced depletion of Se, decrease in plasma testosterone level and partially restored Cd-induced oxidative stress and decrease in sperm count and motility. Taken together, these data suggest that down-regulation of SelP and GPx4 gene expression induces plasma and testicular Se depletion leading, at least in part, to Cd-induced testicular pathophysiology.
Messaoudi
Imed
I
UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000 Monastir, Tunisia. imed messaoudi@yahoo.fr
Banni
Mohamed
M
Saïd
Lamia
L
Saïd
Khaled
K
Kerkeni
Abdelhamid
A
D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000235:genetics*; D015227:Lipid Peroxidation; D008297:Male; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D016133:Polymerase Chain Reaction; D051381:Rats; D051149:Selenoprotein P / Q000235:genetics*; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000503:physiopathology; D013739:Testosterone / Q000097:blood
D017931:DNA Primers; D051149:Selenoprotein P; D002104:Cadmium; D013739:Testosterone; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; C523860:glutathione peroxidase 4, rat