title: Genotoxic, physiological and immunological effects caused by temperature increase, air exposure or food deprivation in freshwater crayfish Astacus leptodactylus.
abstract: The aim of this research was to investigate influence of different environmental stressors, such as temperature increase, air exposure and food deprivation on DNA integrity of a bioindicator species, freshwater crayfish Astacus leptodactylus. DNA damage was measured in crayfish haemocytes using Comet assay and micronucleus test. Crayfish haemolymph was subsequentially sampled during their 7 days of exposure to increased temperatures (25 and 30 degrees C) and during 24 h of air exposure. Both groups were also monitored through the following 7 days of recovery period. Food deprived crayfish were monitored over a period of 2 weeks. Alterations of measured physiological and immunological haemolymph parameters (THC, lactate, glucose and protein concentration) indicated stress response in exposed crayfish. However, only the stress induced by increased temperature significantly increased DNA damage in freshwater crayfish while food deprivation or air exposure did not cause a significant genotoxic effect.
authors.affiliation: School of Environmental Sciences, University of Nova Gorica, Rozna Dolina, 5000 Nova Gorica, Slovenia.
authors.forename: Olga
authors.initials: O
authors.lastname: Malev
authors.forename: Maja
authors.initials: M
authors.lastname: Srut
authors.forename: Ivana
authors.initials: I
authors.lastname: Maguire
authors.forename: Anamaria
authors.initials: A
authors.lastname: Stambuk
authors.forename: Enrico A
authors.initials: EA
authors.lastname: Ferrero
authors.forename: Simonetta
authors.initials: S
authors.lastname: Lorenzon
authors.forename: Göran I V
authors.initials: GI
authors.lastname: Klobucar
mesh_terms: D000388:Air; D000818:Animals; D003400:Astacoidea / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D002452:Cell Count; D004249:DNA Damage; D005508:Food Deprivation; D005618:Fresh Water / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D006434:Hemocytes / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009152:Mutagenicity Tests; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D019344:Lactic Acid; D005947:Glucose