Heme-based sensing by the mammalian circadian protein CLOCK.
Heme is emerging as a key player in the synchrony of circadian-coupled transcriptional regulation. Current evidence suggests that levels of circadian-linked transcription are regulated in response to both the availability of intracellular heme and heme-based sensing of carbon monoxide (CO) and possibly nitric oxide (NO). The protein CLOCK is central to the regulation and maintenance of circadian rhythms in mammals. CLOCK comprises two PAS domains, each with a heme binding site. Our studies focus on the functionality of the murine CLOCK PAS-A domain (residues 103-265). We show that CLOCK PAS-A binds iron(III) protoporhyrin IX to form a complex with 1:1 stoichiometry. Optical absorbance and resonance Raman studies reveal that the heme of ferric CLOCK PAS-A is a six-coordinate, low-spin complex whose resonance Raman signature is insensitive to pH over the range of protein stability. Ferrous CLOCK PAS-A is a mixture of five-coordinate, high-spin and six-coordinate, low-spin complexes. Ferrous CLOCK PAS-A forms complexes with CO and NO. Ferric CLOCK PAS-A undergoes reductive nitrosylation in the presence of NO to generate a CLOCK PAS-A-NO, which is a five-coordinate {FeNO}(7) complex. Formation of the highly stable {FeNO}(7) heme complex from either ferrous or ferric heme makes possible the binding of NO at very low concentration, a characteristic of NO sensors. Comparison of the spectroscopic properties and CO-binding kinetics of CLOCK PAS-A with other CO sensor proteins reveals that CLOCK PAS-A exhibits chemical properties consistent with a heme-based gas sensor protein.
Lukat-Rodgers
Gudrun S
GS
Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58102, USA.
Correia
Cristina
C
Botuyan
Maria Victoria
MV
Mer
Georges
G
Rodgers
Kenton R
KR
D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics / Q000502:physiology*; D002940:Circadian Rhythm; D006418:Heme / Q000502:physiology*; D006801:Humans; D051379:Mice; D013059:Spectrum Analysis, Raman; D014158:Transcription, Genetic
D006418:Heme; D056926:CLOCK Proteins