title: On the design of fluorescent ratiometric nanosensors.
abstract: Advances in nanoparticle technology have recently offered new tools to the bioanalytical field of research. In particular, new nanoparticle-based sensors have appeared able to give quantitative information about different species (ions, metabolites, biomolecules) in biosamples through ratiometric measurements. This article describes the methodologies developed so far in the design of such nanosensors. In particular, the different approaches to immobilize fluorescent chemosensor dyes to nanoparticles are presented. Concept designs of ratiometric nanosensors in terms of composition and architecture are also described and illustrated with examples taken from the literature.
authors.affiliation: Institute of Physical Chemistry, Friedrich Schiller University of Jena, Lessingstrasse 10, 07743, Jena, Germany. tristan.doussineau@uni-jena.de
authors.forename: Tristan
authors.initials: T
authors.lastname: Doussineau
authors.forename: Anja
authors.initials: A
authors.lastname: Schulz
authors.forename: Alejandro
authors.initials: A
authors.lastname: Lapresta-Fernandez
authors.forename: Artur
authors.initials: A
authors.lastname: Moro
authors.forename: Susanne
authors.initials: S
authors.lastname: Körsten
authors.forename: Sabine
authors.initials: S
authors.lastname: Trupp
authors.forename: Gerhard J
authors.initials: GJ
authors.lastname: Mohr
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D013050:Spectrometry, Fluorescence
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes