title: Methacrylate ester-based monolithic columns for nano-LC separation of tocopherols in vegetable oils.
abstract: The separation and determination of tocopherols (Ts) in vegetable oils by nano-LC chromatography with UV-vis detection using lauryl methacrylate ester-based monolithic columns has been developed. The separation of Ts was optimized in terms of mobile phase composition on the basis of the best compromise among efficiency, resolution and analysis time. Using a mobile phase composed of ACN/methanol/water, an excellent resolution between Ts was achieved within 18 min. The LODs were lower than 0.26 μg/mL, being repeatability values of retention time and peak area below 0.15 and 3.1%, respectively. The method was applied to the quantification of Ts and tocotrienols present in several vegetable oils from different botanical origins.
authors.affiliation: Departamento de Química Analítica, Universidad de Valencia, Valencia, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lerma-García
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Cerretani
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bendini
authors.forename: Ernesto Francisco
authors.initials: EF
authors.lastname: Simó-Alfonso
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004952:Esters / Q000737:chemistry*; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D024505:Tocopherols / Q000032:analysis*
chemical_list: D004952:Esters; D008689:Methacrylates; D010938:Plant Oils; D012997:Solvents; D024505:Tocopherols