A novel gene delivery system targeting Tie2 for cancer gene therapy.
The aim of this study was to explore a novel gene vector for targeting gene therapy.


METHODS
We conjugated a peptide ligand (named GA3) for endothelial TEK tyrosine kinase (Tie2) with polyethylenimine (PEI) to construct a GA3-PEI complex and used the vector to transfer reporter and therapeutic gene in vitro and in vivo respectively.


RESULTS
The results demonstrated the vehicle was able to transfer reporter genes specifically into lung cancer SPC-A1 cells and SPC-A1 xenografts highly expressing Tie2 and epithelial cells of bronchus, but not in heart, liver, spleen, kidney, lung alveolar and vascular tissues. In the gene therapy study, tumor growth was significantly inhibited in SPC-A1 xenograft-bearing mice treated with GA3-PEI/p53 complexes compared with control groups (p<0.05).


CONCLUSIONS
Our results indicated that GA3-PEI is an efficient gene delivery system targeting Tie2.
Hu
Aiqun
A
Department of Medical Oncology, Cancer Hospital of Fudan University, Department of Oncology, Fudan University, Shanghai, China.
Wu
Xianghua
X
Li
Zonghai
Z
Wang
Huamao
H
Li
Jingjun
J
Yao
Ming
M
Chen
Xianlian
X
Qu
Shumin
S
Xu
Yuhong
Y
Gu
Jianren
J
D000818:Animals; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D011094:Polyethyleneimine / Q000008:administration & dosage; D042787:Receptor, TIE-2 / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
D011094:Polyethyleneimine; D042787:Receptor, TIE-2