title: Effect of tertiary butylhydroperoxide-induced oxidative stress on glutathione content and thiobarbituric acid reactive substances production in cultures and co-cultures of adult rat hepatocytes.
abstract: The production of thiobarbituric acid reactive substances (TBARS) and the content of reduced glutathione (GSH) were determined in primary monolayer cultures of adult rat hepatocytes and in their co-cultures with rat epithelial cells, both exposed for 90 min to 2 mm tertiary butylhydroperoxide. Under these stress-induced conditions, TBARS production tended to be less increased in co-cultured cells than in conventionally cultured hepatocytes. A total depletion of GSH content was measured in conventional cultures from the third culture day, whereas in co-cultures a decrease in GSH content was observed until the third day of culture, followed by a low but constant GSH level that was maintained until the end of the culture period. Oxidative stress appears to be less pronounced in co-cultures than in conventional cultures; the former cope better with externally induced oxidative stress than do the latter.
authors.affiliation: Free University of Brussels, Faculty of Medicine and Pharmacy, Department of Toxicology and Biostatistics, Laarbeeklaan 103, B-1090 Brussels, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Mertens
authors.forename: S
authors.initials: S
authors.lastname: Kaufman
authors.forename: S
authors.initials: S
authors.lastname: Waterschoot
authors.forename: A
authors.initials: A
authors.lastname: Vercruysse
authors.forename: V
authors.initials: V
authors.lastname: Rogiers