title: Electrochemical investigations of cytochrome P450.
abstract: In this paper we summarized our experimental data on the electrochemical reduction of cytochrome P450. Electrode/cytochrome P450 systems were analyzed in terms of the mechanisms underlying P450-catalyzed reactions. Bioelectrocatalysis-based screening of potential substrates or inhibitors of cytochrome P450, stoichiometry of the electrocatalytic cycle, redox thermodynamics and the peroxide shunt pathway were described. Characteristics, performance and potential application of cytochrome P450-electrodes are discussed.
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya St. 10, Moscow 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Shumyantseva
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Bulko
authors.forename: Elena V
authors.initials: EV
authors.lastname: Suprun
authors.forename: Yaroslava M
authors.initials: YM
authors.lastname: Chalenko
authors.forename: Michail Yu
authors.initials: MY
authors.lastname: Vagin
authors.forename: Yurii O
authors.initials: YO
authors.lastname: Rudakov
authors.forename: Marina A
authors.initials: MA
authors.lastname: Shatskaya
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D055162:Biocatalysis / Q000187:drug effects; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D000072467:Cytochrome P450 Family 2; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008797:Metyrapone / Q000494:pharmacology; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects; D011817:Rabbits; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit); D008797:Metyrapone