Shock wave-pretreated bone marrow cells further improve left ventricular function after myocardial infarction in rabbits.
BACKGROUND
We tested whether shock wave (SW) offers additional benefits in improving left ventricular (LV) function after acute myocardial infarction (AMI) in rabbits receiving SW-treated autologous bone marrow-derived mononuclear cells (BMDMNCs) transplantation.


RESULTS
Saline (750 microL; group 2), BMDMNCs (1.0 x 10(7); group 3), or preimplant SW-treated BMDMNCs (group 4) were implanted into the infarct area of male rabbits 15 minutes after left coronary artery ligation, whereas eight rabbits without AMI served as controls (group 1; n = 8 per group). The results showed that in infarct area of LV, protein expressions of Cx43 and cytochrome C in mitochondria and endothelial nitric oxide synthase mRNA expression were lower in group 2 than in other groups, and decreased in group 3 as compared with groups 1 and 4 (all p values < 0.01). Conversely, mRNA expressions of endothelin-1 and matrix metalloproteinase-9, mitochondrial oxidative stress, and total fibrotic area were higher in group 2 than in other groups (all p values < 0.05). Furthermore, 6-month LV function by 2-D echo/angiogram showed significant impairment in group 2 than in other groups and in group 3 than in groups 1 and 4 (all p values < 0.005).


CONCLUSIONS
Application of SW-treated autologous BMDMNCs is superior to BMDMNCs alone for preserving LV function after AMI.
Sheu
Jiunn-Jye
JJ
Division of Cardiovascular and Thoracic Surgery, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University College of Medicine, Kaohsiung, Taiwan, ROC.
Sun
Cheuk-Kwan
CK
Chang
Li-Teh
LT
Fang
Hsiu-Yu
HY
Chung
Sheng-Ying
SY
Chua
Sarah
S
Fu
Morgan
M
Lee
Fan-Yen
FY
Kao
Ying-Hsien
YH
Ko
Sheung-Fat
SF
Wang
Ching-Jen
CJ
Yen
Chia-Hung
CH
Leu
Steve
S
Yip
Hon-Kan
HK
D000818:Animals; D001854:Bone Marrow Cells / Q000528:radiation effects*; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D018031:Connexin 43 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000235:genetics; D005355:Fibrosis; D005786:Gene Expression Regulation; D019220:High-Energy Shock Waves; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D008929:Mitochondria, Heart / Q000378:metabolism; D009203:Myocardial Infarction / Q000175:diagnosis / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D052250:Nitric Oxide Synthase Type III / Q000235:genetics; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D020127:Recovery of Function; D013997:Time Factors; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left
D018031:Connexin 43; D019332:Endothelin-1; D012333:RNA, Messenger; D045304:Cytochromes c; D052250:Nitric Oxide Synthase Type III; D020780:Matrix Metalloproteinase 9