Bone regeneration by lactoferrin released from a gelatin hydrogel.
The objective of this study is to evaluate the potential of lactoferrin (LF), an iron-binding glycoprotein, to induce bone regeneration. A biodegradable gelatin hydrogel was prepared to allow LF release in vivo in a sustained fashion. When subcutaneously implanted into the back of mice, the gelatin hydrogel incorporating LF showed a longer LF retention period at the site of implantation than that of LF solution injection. An in vitro culture experiment with 3T3E1 cells (mouse-derived osteoblasts) revealed that the cells were proliferated to a significantly greater extent by the repeated addition of LF compared with a single addition of LF at the same dose. Following the implantation of gelatin hydrogels incorporating LF into a skull bone defect of rats, a significantly stronger bone regeneration at the defect was observed than in LF-free- or low-LF-treated rats. It is concluded that the sustained release from the gelatin hydrogels enables LF to enhance the in vivo activity of bone regeneration.
Takaoka
Ryohei
R
Institute for Frontier Medical Sciences, Kyoto University, Kawara-cho Shogoin, Sakyo-ku, Japan.
Hikasa
Yoshiaki
Y
Hayashi
Kentaro
K
Tabata
Yasuhiko
Y
D016475:3T3 Cells; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations; D005260:Female; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D012886:Skull / Q000187:drug effects / Q000503:physiopathology
D003692:Delayed-Action Preparations; D020100:Hydrogels; D005780:Gelatin; D007781:Lactoferrin