Probing protease activity by single-fluorescent-protein nanocapsules.
We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.
Gu
Zhen
Z
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA.
Biswas
Anuradha
A
Joo
Kye-Il
KI
Hu
Biliang
B
Wang
Pin
P
Tang
Yi
Y
D000595:Amino Acid Sequence; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
D053769:Nanocapsules; D010447:Peptide Hydrolases