title: Global transcriptome profiling of wild soybean (Glycine soja) roots under NaHCO3 treatment.
abstract: BACKGROUND
  Plant roots are the primary site of perception and injury for saline-alkaline stress. The current knowledge of saline-alkaline stress transcriptome is mostly focused on saline (NaCl) stress and only limited information on alkaline (NaHCO3) stress is available.
  
  
  RESULTS
  Using Affymetrix Soybean GeneChip, we conducted transcriptional profiling on Glycine soja roots subjected to 50 mmol/L NaHCO3 treatment. In a total of 7088 probe sets, 3307 were up-regulated and 5720 were down-regulated at various time points. The number of significantly stress regulated genes increased dramatically after 3 h stress treatment and peaked at 6 h. GO enrichment test revealed that most of the differentially expressed genes were involved in signal transduction, energy, transcription, secondary metabolism, transporter, disease and defence response. We also detected 11 microRNAs regulated by NaHCO3 stress.
  
  
  CONCLUSIONS
  This is the first comprehensive wild soybean root transcriptome analysis under alkaline stress. These analyses have identified an inventory of genes with altered expression regulated by alkaline stress. The data extend the current understanding of wild soybean alkali stress response by providing a set of robustly selected, differentially expressed genes for further investigation.
authors.affiliation: Plant Bioengineering Laboratory, The College of Life Sciences, Northeast Agricultural University, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ge
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Zhu
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
authors.forename: De-Kang
authors.initials: DK
authors.lastname: Lv
authors.forename: Dianjing
authors.initials: D
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*
chemical_list: D017693:Sodium Bicarbonate