[Expression of human beta-defensin after endoscopic sinus surgery for chronic sinusitis].
OBJECTIVE
To observe the expressions of human beta-defensin 1 and 2 (hBD-1 and hBD-2) in nasal mucosa before and after the endoscopic sinus surgery and investigate the effects of hBD-1 and hBD-2 on the healing process after the surgery.


METHODS
The patients undergoing endoscopic sinus surgery for chronic sinusitis were divided into 3 groups according to the response to the surgery, namely cured group, response group and non-response group. With those from healthy control subjects as the control, nasal mucosa samples were collected from the patients at 1 week, 2 weeks, 1 month, 3 months and 6 months after the surgery for detection of hBD-1 and hBD-2 mRNA and protein expressions by RT-PCR and Western blotting.


RESULTS
hBD-1 and hBD-2 were expressed in both normal and chronic sinusitis mucosa, but the expression levels varied significantly between the individuals. The expression levels of hBD-2 was significantly correlated to the patients' response to the surgical treatment (P<0.05). hBD-1 showed slight differences between the individuals, but was not associated with the patients' prognosis.


CONCLUSIONS
The expressions of hBD-2 mRNA and protein are significantly increased in patients with good response to endoscopic sinus surgery for chronic sinusitis, suggesting the value of hBD-2 as an indicator of the patients' prognosis.
Xiao
Chu-zhi
CZ
Department of Otolaryngology and Head-Neck Surgery, Yangang Hospital, Shenzhen 518083, China. xcz1962@126.com
He
Guang-xiang
GX
Deng
Wei-guang
WG
Zhang
Hai-ying
HY
Sun
Wen
W
D016022:Case-Control Studies; D002908:Chronic Disease; D004724:Endoscopy; D006801:Humans; D009297:Nasal Mucosa / Q000378:metabolism*; D011184:Postoperative Period; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012852:Sinusitis / Q000378:metabolism* / Q000601:surgery; D023083:beta-Defensins / Q000378:metabolism*
C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins