Handheld impedance biosensor system using engineered proteinaceous receptors.
We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.
Ghafar-Zadeh
Ebrahim
E
Department of Electrical and Computer Engineering, McGill University, Montreal, Canada. ebrahim.ghafar-zadeh@polymtl.ca
Chowdhury
Shafinaz F
SF
Aliakbar
Amir
A
Chodavarapu
Vamsy
V
Lumbroso
Rose
R
Lambrose
Rosemond
R
Beitel
Lenore K
LK
Beital
Lenord
L
Sawan
Mohamad
M
Trifiro
Mark
M
D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004867:Equipment Design; D005941:Glucokinase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D015202:Protein Engineering; D012984:Software; D013997:Time Factors
D004800:Enzymes, Immobilized; D006046:Gold; D005941:Glucokinase; D005947:Glucose