Characterization and phylogenetic analysis of cellulase producing Streptomyces noboritoensis SPKC1.
A cellulase producing strain of Actinomycetes was isolated from soil samples which were collected from Southwest ghats, Kerala, India at a depth of 6-12 inches and Actinomycetes was characterized by morphological, cultural, physiological, chemotaxonomical and phylogenetic analysis. The 16S rRNA region of this strain was amplified and sequenced. The Neighbor-joining and Maximum Parsimony algorithm with topology tree of 16S rRNA was constructed. Based on results of observation and phylogenetic analysis, the strain SPKC1 was proved to belong to the species Streptomyces noboritoensis with cellulase activity. The Carboxy Methyl Cellulose (CMCase) activities of the strain SPKC1 on eighth day an amount of 910 microg/ml of glucose, 210 microg/ml of protein and 850 mg/100 ml of growth (biomass) on ninth day were recorded. In exocellulase activity strain SPKC1 on first day an amount of 500 microg/ml glucose was produced.
George
Jiji
J
Division of Microbiology, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala, India.
Arunachalam
Ramaiah
R
Paulkumar
Kanniah
K
Wesely
E G
EG
Shiburaj
S
S
Annadurai
Gurusamy
G
D000465:Algorithms; D002244:Carbon / Q000737:chemistry; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000235:genetics; D005285:Fermentation; D008828:Microbiological Techniques; D008957:Models, Genetic; D010802:Phylogeny; D011506:Proteins / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D002482:Cellulose; D002480:Cellulase