title: Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring method.
abstract: Recently, our group discovered an alternative titanium dioxide (TiO(2)) activation method that uses ultrasound irradiation (US/TiO(2)) instead of ultraviolet irradiation. The pre-S1/S2 protein from hepatitis B virus, which recognizes liver cells, was immobilized to the surface of TiO(2) nanoparticles using an amino-coupling method. The ability of the protein-modified TiO(2) nanoparticles to recognize liver cells was confirmed by surface plasmon resonance analysis and immuno-staining analyses. After uptake of TiO(2) nanoparticles by HepG2 cancer cells, the cells were injured using this US/TiO(2) method; significant cell injury was observed at an ultrasound irradiation intensity of 0.4 W/cm(2). Together with these results, this strategy could be applied to new cell injuring systems that use ultrasound irradiation in place of photodynamic therapy in the near future.
authors.affiliation: The Division of Material Sciences, Graduate School of Neutral Science and Technology, Kanazawa University, Kakuma, Kanazawa, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Naonori
authors.initials: N
authors.lastname: Shibata
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Sasai
authors.forename: Keiko
authors.initials: K
authors.lastname: Takaki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyachi
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Kuroda
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry / Q000302:isolation & purification*; D014465:Ultrasonics
chemical_list: D011506:Proteins; C009495:titanium dioxide; D014025:Titanium