title: Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging.
abstract: A method that provides maps of absolute concentrations of oxygenated and deoxygenated myoglobin (Mb), its oxygenation, and its near-infrared (NIR) optical pathlength in cardiac tissue was developed. These parameters are available simultaneously. The method is based on NIR diffuse reflectance spectroscopic imaging and specific processing of the NIR images, which included a first derivative of the diffuse reflectance spectrum. Mb oxygenation, total Mb concentration, and NIR light pathlength were found to be in the range of 92%, 0.3 mM, and 12.5 mm, respectively, in beating isolated buffer-perfused and arrested pig hearts. The charge-coupled device camera enables sub-millimeter spatial resolution and spectroscopic imaging in 1.5 to 2.0 min. The technique is noninvasive and nondestructive. The equipment has no mechanical contact with the tissue of interest, leaving it undisturbed.
authors.affiliation: National Research Council of Canada Institute for Biodiagnostics, Winnipeg, Manitoba, Canada R3B 1Y6. eugene.gussakovsky@nrc-cnrc.gc.ca
authors.forename: Eugene
authors.initials: E
authors.lastname: Gussakovsky
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Yang
authors.forename: John
authors.initials: J
authors.lastname: Rendell
authors.forename: Olga
authors.initials: O
authors.lastname: Jilkina
authors.forename: Valery
authors.initials: V
authors.lastname: Kupriyanov
mesh_terms: D000818:Animals; D007259:Infrared Rays; D008962:Models, Theoretical; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013552:Swine; D014741:Video Recording
chemical_list: D009211:Myoglobin; D010100:Oxygen