title: Novel SNPs of the caprine growth hormone secretagogue receptor (GHSR) gene and their association with growth traits in goats.
abstract: Growth hormone secretagogue receptor (GHSR), a G protein-coupled receptor that binds ghrelin, plays an important role in the central regulation of pituitary growth hormone secretion, food intake, and energy homeostasis. This study analyzed polymorphism of the caprine GHSR gene as a genetic marker candidate for growth traits in goats. Two single nucleotide polymorphisms (GU014697:g.165G-->A and GU014697:g.548T-->C) were identified in exon 2 of the caprine GHSR gene by PCR-single-strand conformation polymorphism and DNA sequencing methods. Their associations with growth traits were analyzed in 313 Xuhuai goats. The results indicated that GU014697:g.548T-->C had significant effects on growth traits. Body length and body length index were significantly higher in individuals with genotype TT than CC and CT in (P < 0.05). TT individuals also tended to have better performance in other traits, such as body height and chest circumference, although there were no statistical differences (P > 0.05). This suggests that GHSR is a strong candidate gene that affects growth traits in goats.
authors.affiliation: Institute of Cellular and Molecular Biology, Xuzhou Normal University, 101 Shanghai Road, Xuzhou, Jiangsu, 221116, China. open3377@126.com
authors.forename: Qijiang
authors.initials: Q
authors.lastname: Jin
authors.forename: Xing Tang
authors.initials: XT
authors.lastname: Fang
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Chun Lei
authors.initials: CL
authors.lastname: Zhang
authors.forename: Jia Jie
authors.initials: JJ
authors.lastname: Sun
authors.forename: Dan Xia
authors.initials: DX
authors.lastname: Chen
authors.forename: X Y
authors.initials: XY
authors.lastname: Shi
authors.forename: Yu
authors.initials: Y
authors.lastname: Du
authors.forename: Chuchao
authors.initials: C
authors.lastname: Lei
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D056426:Genetic Loci; D005838:Genotype; D006041:Goats / Q000235:genetics* / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D054440:Receptors, Ghrelin / Q000235:genetics*
chemical_list: D054440:Receptors, Ghrelin