Hyperpolarization of human mesenchymal stem cells in response to magnetic force.
Magnetic particle tagging techniques are currently being applied to tissue engineering applications such as controlled differentiation of mesenchymal stem cells (MSC). In order to define key mechanotransducers underpinning these applications, the electrophysiological responses of human MSCs (hMSC) have been investigated. Ferromagnetic microparticles were coated with L-arginyl-glycyl-L-aspartic acid in order to target the application of dynamic force (6 pN) directly to cell surface integrins. Human MSCs demonstrated cell membrane hyperpolarization responses after the application of force, mediated by BK channels and intracellular calcium release.
Kirkham
Glen R
GR
Institute of Science and Technology in Medicine, University of Keele, Stoke-on-Trent, ST4 7QB, UK. glen.kirkham@nottingham.ac.uk
Elliot
Kerry J
KJ
Keramane
Ahmed
A
Salter
Donald M
DM
Dobson
Jon P
JP
El Haj
Alicia J
AJ
Cartmell
Sarah Harriet
SH
D000704:Analysis of Variance; D015220:Calcium Channels; D016764:Cell Polarity / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D004594:Electrophysiology / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009543:Nifedipine / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology
D015220:Calcium Channels; D005290:Ferric Compounds; D051036:Large-Conductance Calcium-Activated Potassium Channels; C001215:ferrite; D019284:Thapsigargin; D009543:Nifedipine