A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of escin Ia and escin Ib in human plasma: application to a pharmacokinetic study after intravenous administration.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of escin Ia and escin Ib in human plasma. After a solid-phase extraction (SPE), the analytes were separated on a Zorbax Extend C(18) column by isocratic elution with a mobile phase of methanol-acetonitrile-10 mm ammonium acetate (27:27:46, v/v/v) at a flow rate of 1.0 mL/min and analyzed by mass spectrometry in the positive ion multiple reaction monitoring mode. The precursor to product ion transitions of m/z 1131.8 → 807.6 was used to quantify escin Ia and escin Ib. Good linearity was achieved over a wide range of 2.00-900 ng/mL for escin Ia and 1.50-662 ng/mL for escin Ib. The intra- and inter-day precisions (as relative standard deviation) were less than 11% for each QC level of escin Ia and escin Ib. The accuracies (as relative error) were within ±5.27% for escin Ia and within ±4.07% for escin Ib. The method was successfully employed in a pharmacokinetic study after a single intravenous infusion administration of sodium aescinate injection containing 10 mg escin to each of the 10 healthy volunteers.
Liu
Lidong
L
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130021, People's Republic of China.
Wu
Xiujun
X
Wu
Dan
D
Wang
Yingwu
Y
Li
Pengfei
P
Sun
Yantong
Y
Yang
Yan
Y
Gu
Jingkai
J
Cui
Yimin
Y
D002853:Chromatography, Liquid / Q000379:methods*; D004928:Escin / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
C409970:escin Ib; D004928:Escin; C115101:escin Ia