Enzymatic activity and thermal stability of metallo proteins in hydrated ionic liquids.
Hydrated choline dihydrogen phosphate (Hy[ch][dhp]) containing 30 wt% water was investigated as a novel protein solvent. The Hy[ch][dhp] dissolved some metallo proteins (cytochrome c, peroxidase, ascorbate oxidase, azurin, pseudoazurin and fructose dehydrogenase) without any modification. These proteins retained the surroundings of the active site after dissolution in Hy[ch][dhp]. Some metallo proteins were found to retain their activity in the Hy[ch][dhp].
Fujita
Kyoko
K
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo184-8588, Japan.
Ohno
Hiroyuki
H
D000818:Animals; D001204:Ascorbate Oxidase / Q000737:chemistry / Q000378:metabolism; D001400:Azurin / Q000737:chemistry / Q000378:metabolism; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D052578:Ionic Liquids / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D055550:Protein Stability; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013696:Temperature
D004798:Enzymes; D052578:Ionic Liquids; D008667:Metalloproteins; D010710:Phosphates; D012997:Solvents; C047163:pseudoazurin; D001400:Azurin; D005632:Fructose; D045304:Cytochromes c; D002237:Carbohydrate Dehydrogenases; D001204:Ascorbate Oxidase; D009195:Peroxidase