title: The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.
abstract: The NineTeen Complex (NTC) of proteins associates with the spliceosome during pre-mRNA splicing and is essential for both steps of intron removal. The NTC and other NTC-associated proteins are recruited to the spliceosome where they participate in regulating the formation and progression of essential spliceosome conformations required for the two steps of splicing. It is now clear that the NTC is an integral component of active spliceosomes from yeast to humans and provides essential support for the spliceosomal snRNPs (small nuclear ribonucleoproteins). In the present article, we discuss the identification and characterization of the yeast NTC and review recent work in yeast that supports the essential role for this complex in the regulation and fidelity of splicing.
authors.affiliation: Faculty of Life Sciences, Michael Smith Building, The University of Manchester, Manchester M13 9PT, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hogg
authors.forename: Joanne C
authors.initials: JC
authors.lastname: McGrail
authors.forename: Raymond T
authors.initials: RT
authors.lastname: O'Keefe
mesh_terms: D006801:Humans; D008954:Models, Biological; D008968:Molecular Conformation; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing / Q000502:physiology*; D000072260:RNA Splicing Factors; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D017381:Spliceosomes / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*
chemical_list: C079877:PRP19 protein, S cerevisiae; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D029701:Saccharomyces cerevisiae Proteins; C507652:Spp382 protein, S cerevisiae