title: Effects of water extract of Cajanus cajan leaves on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and the adipocytic trans-differentiation of mouse primary osteoblasts.
abstract: The effects of water extract of Cajanus cajan (Linn.) Millsp. (Leguminosae) leaves (WECML) on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipocytic trans-differentiation of mouse primary osteoblasts (OBs) were studied. The results indicated that WECML promoted the proliferation of BMSCs and OBs at most concentrations. WECML promoted the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentrations of 0.1, 1, and 10 microg/mL, but inhibited the osteogenic differentiation and formation of mineralized matrix nodules of BMSCs at concentration of 0.01 microg/mL. WECML inhibited the adipogenic differentiation of BMSCs and adipocytic trans-differentiation of OBs at concentrations of 0.001, 0.1, 1, 10, and 100 microg/mL, but had no effects at concentration of 0.01 microg/mL. The results suggest that WECML has protective effects on bone and these protective effects may be mediated by decreasing adipocytic cell formation from BMSCs, which may promote the proliferation, differentiation, and mineralization function of OBs. The defined active ingredients in the WECML and the active mechanism need to be further studied.
authors.affiliation: College of Chemistry and Environmental Science, Chemical Biology Laboratory, Hebei University, Baoding, PR China. jczhang6970@yahoo.com.cn
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Cuilian
authors.initials: C
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D036561:Cajanus; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D054338:Cell Transdifferentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D014867:Water / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D014867:Water