Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.
Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.
Peruzzi
Benedetta
B
Laboratory of Genetics and Gene Transfer, Core Research Laboratory, Istituto Toscano Tumori (ITT), AOU Careggi, Firenze, Italy.
Serra
Martina
M
Pescucci
Chiara
C
Sica
Michela
M
Lastraioli
Sonia
S
Rondelli
Tommaso
T
Pedemonte
Simona
S
Risitano
Antonio Maria
AM
De Angioletti
Maria
M
Piccioli
Patrizia
P
Notaro
Rosario
R
D000483:Alleles; D002460:Cell Line; D003176:Complement C3 / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
D003176:Complement C3; D017931:DNA Primers