title: Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid.
abstract: A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid.
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Gallardo
authors.forename: María
authors.initials: M
authors.lastname: Fernández-Fernández
authors.forename: Cristina
authors.initials: C
authors.lastname: Valls
authors.forename: Susana Valeria
authors.initials: SV
authors.lastname: Valenzuela
authors.forename: M Blanca
authors.initials: MB
authors.lastname: Roncero
authors.forename: Teresa
authors.initials: T
authors.lastname: Vidal
authors.forename: Pilar
authors.initials: P
authors.lastname: Díaz
authors.forename: F I Javier
authors.initials: FI
authors.lastname: Pastor
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D057886:Bleaching Agents / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D005052:Eucalyptus / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D057886:Bleaching Agents; D009844:Oligosaccharides; C076151:Kraft lignin; D008031:Lignin; D014995:Xylosidases