Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68.
Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) and provides a small-animal model with which to study the pathogenesis of gammaherpesvirus (gammaHV) infections. To completely explore the potential of the MHV-68 system for the investigation of gammaHV microRNAs (miRNAs), it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By deep sequencing of small RNAs, we systematically investigated the expression profiles of MHV-68 miRNAs in both lytically and persistently infected cells. In addition to the nine known MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68-infected versus noninfected NIH 3T3 fibroblasts and in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated versus nontreated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH 3T3 cells, indicating a potential role for cellular miRNAs during MHV-68 infection. Our data will aid in the full exploration of the functions of gammaHV miRNAs.
Zhu
Jia Yun
JY
Center for Integrated Protein Sciences Munich, Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
Strehle
Martin
M
Frohn
Anne
A
Kremmer
Elisabeth
E
Höfig
Kai P
KP
Meister
Gunter
G
Adler
Heiko
H
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D016679:Genome, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D018144:Rhadinovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D017423:Sequence Analysis, RNA
D017931:DNA Primers; D035683:MicroRNAs; D012367:RNA, Viral