Excess neutrophil infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice.
Wild-type mice control murine cytomegalovirus (MCMV) brain infection, but identical infection is lethal to animals deficient in interleukin (IL)-10. Here, we report that MCMV-infected IL-10 knockout (KO) mice displayed a marked increase in neutrophil infiltration into the infected, IL-10-deficient brain when compared to wild-type animals. Enhanced microglial cell activation, determined by MHC class II up-regulation, overexpression of CXCL2, and elevated P-selectin mRNA levels were observed. In vivo blocking of CXCL2 attenuated neutrophil infiltration and significantly improved the outcome of infection. Collectively, these data indicate that the absence of IL-10 results in pathologic neutrophil infiltration into MCMV-infected brains.
Mutnal
Manohar B
MB
Neuroimmunology Laboratory, Center for Infectious Diseases and Microbiology Translational Research, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Cheeran
Maxim C-J
MC
Hu
Shuxian
S
Little
Morgan R
MR
Lokensgard
James R
JR
D000818:Animals; D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D018792:Encephalitis, Viral / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D018146:Muromegalovirus / Q000276:immunology*; D041681:NIH 3T3 Cells; D020556:Neutrophil Infiltration / Q000235:genetics / Q000276:immunology*; D051381:Rats
D016753:Interleukin-10