The heat shock protein YbeY is required for optimal activity of the 30S ribosomal subunit.
The highly conserved bacterial ybeY gene is a heat shock gene whose function is not fully understood. Previously, we showed that the YbeY protein is involved in protein synthesis, as Escherichia coli mutants with ybeY deleted exhibit severe translational defects in vivo. Here we show that the in vitro activity of the translation machinery of ybeY deletion mutants is significantly lower than that of the wild type. We also show that the lower efficiency of the translation machinery is due to impaired 30S small ribosomal subunits.
Rasouly
Aviram
A
Department of Molecular Microbiology and Biotechnology, The George S Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel.
Davidovich
Chen
C
Ron
Eliora Z
EZ
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D008667:Metalloproteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D054680:Ribosome Subunits, Small, Bacterial / Q000235:genetics / Q000378:metabolism*
D029968:Escherichia coli Proteins; D008667:Metalloproteins; C510617:YbeY protein, E coli