title: Prolonged activation of NMDA receptors promotes dephosphorylation and alters postendocytic sorting of GABAB receptors.
abstract: Slow and persistent synaptic inhibition is mediated by metabotropic GABAB receptors (GABABRs). GABABRs are responsible for the modulation of neurotransmitter release from presynaptic terminals and for hyperpolarization at postsynaptic sites. Postsynaptic GABABRs are predominantly found on dendritic spines, adjacent to excitatory synapses, but the control of their plasma membrane availability is still controversial. Here, we explore the role of glutamate receptor activation in regulating the function and surface availability of GABABRs in central neurons. We demonstrate that prolonged activation of NMDA receptors (NMDA-Rs) leads to endocytosis, a diversion from a recycling route, and subsequent lysosomal degradation of GABABRs. These sorting events are paralleled by a reduction in GABABR-dependent activation of inwardly rectifying K+ channel currents. Postendocytic sorting is critically dependent on phosphorylation of serine 783 (S783) within the GABABR2 subunit, an established substrate of AMP-dependent protein kinase (AMPK). NMDA-R activation leads to a rapid increase in phosphorylation of S783, followed by a slower dephosphorylation, which results from the activity of AMPK and protein phosphatase 2A, respectively. Agonist activation of GABABRs counters the effects of NMDA. Thus, NMDA-R activation alters the phosphorylation state of S783 and acts as a molecular switch to decrease the abundance of GABABRs at the neuronal plasma membrane. Such a mechanism may be of significance during synaptic plasticity or pathological conditions, such as ischemia or epilepsy, which lead to prolonged activation of glutamate receptors.
authors.affiliation: Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA.
authors.forename: Miho
authors.initials: M
authors.lastname: Terunuma
authors.forename: Karina J
authors.initials: KJ
authors.lastname: Vargas
authors.forename: Megan E
authors.initials: ME
authors.lastname: Wilkins
authors.forename: Omar A
authors.initials: OA
authors.lastname: Ramírez
authors.forename: Matías
authors.initials: M
authors.lastname: Jaureguiberry-Bravo
authors.forename: Menelas N
authors.initials: MN
authors.lastname: Pangalos
authors.forename: Trevor G
authors.initials: TG
authors.lastname: Smart
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Moss
authors.forename: Andrés
authors.initials: A
authors.lastname: Couve
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004705:Endocytosis; D005260:Female; D006801:Humans; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*
chemical_list: D018080:Receptors, GABA-B; D016194:Receptors, N-Methyl-D-Aspartate; D055372:AMP-Activated Protein Kinases