title: Determination of Fusarium mycotoxins by liquid chromatography/tandem mass spectrometry coupled with immunoaffinity extraction.
abstract: A method for the simultaneous quantitative determination of deoxynivalenol (DON), T-2 toxin (T-2), HT-2 toxin (HT-2) and zearalenone (ZEN) in wheat and biscuit by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) coupled with immunoaffinity extraction is described. A clean-up was carried out using a DZT MS-PREP immunoaffinity column (IAC), and the effect of the sample dilution rate and sample loading was investigated. Furthermore, the effects of ion suppression of a multifunctional column (MFC) and the IAC in the clean-up were compared. The results with the DZT MS-PREP IAC showed that it is possible to make the sample dilution rate low, and indicated a higher solvent-tolerance than usual with an IAC. Sample loading was optimized at 0.25 g. Ion suppression was lowered by purification of the toxins using the DZT MS-PREP IAC. Recoveries of each mycotoxin from wheat and biscuit samples spiked at two levels ranged from 78 to 109%. The limits of detection in wheat and biscuit was in the range of 0.03-0.33 ng x g(-1). From these studies, it is suggested that use of an IAC is effective in the clean-up of each mycotoxin, and, when combined with LC/ESI-MS/MS, it is good for the determination of mycotoxins in foodstuffs due to its rapidity and high sensitivity.
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. hiroki.t@poem.ocn.ne.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Masahiko
authors.initials: M
authors.lastname: Takino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Tanaka
authors.forename: David
authors.initials: D
authors.lastname: Leeman
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005670:Fusarium / Q000737:chemistry / Q000378:metabolism; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry*
chemical_list: D009183:Mycotoxins