title: Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma.
abstract: During an allergic inflammatory response in the airway, if a failure of the epithelial cell barrier occurs before the systemic immune response is triggered by allergens, more allergens can invade. Using a rat model of asthma, we previously found that mucosal mast cells, which localise to the epithelial layer of the airways, are activated to promote a pro-asthmatic immune response. In this study, we developed a neonatal rat model of allergic airway hypersensitivity that mimics some features of childhood asthma. Airway hypersensitivity was measured using unrestrained whole-body plethysmography after analysis of the serum IgE titre. Inflammatory cells and inflammatory mediators in bronchoalveolar lavage fluid samples were examined. Two mast cell-specific proteases were detected using PCR. In addition, we analysed the phenotype and the number of mast cells in the airways by immunohistochemistry, and we found that the number of mucosal mast cells and the expression level of the proteases increased 2 weeks after sensitisation. Changes in the IgE titre, airway hypersensitivity and the activation of other inflammatory cells were delayed, appearing during the 4 weeks after sensitisation. Our results indicate that the activation of mucosal mast cells contributes to the pro-asthmatic immune response. This activation may be a biomarker allowing early intervention that could help prevent allergic airway inflammation.
authors.affiliation: Department of Pharmacology, Information Biomedicine, Ehime University School of Medicine, Ehime, Japan. liussmzk@m.ehime-u.ac.jp
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Masachika
authors.initials: M
authors.lastname: Shudou
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Maeyama
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001249:Asthma / Q000097:blood / Q000150:complications / Q000276:immunology* / Q000473:pathology; D001980:Bronchi / Q000201:enzymology / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002452:Cell Count; D002648:Child; D004195:Disease Models, Animal; D006801:Humans; D007109:Immunity / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D008407:Mast Cells / Q000201:enzymology / Q000276:immunology*; D010447:Peptide Hydrolases / Q000378:metabolism; D011014:Pneumonia / Q000097:blood / Q000150:complications / Q000276:immunology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000276:immunology* / Q000473:pathology*; D053802:Tryptases / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D007073:Immunoglobulin E; D010447:Peptide Hydrolases; D053802:Tryptases