[Effect of hBMP-7 gene modified bone marrow stromal cells on periodontal tissue regeneration].
OBJECTIVE
To estimate the potential effects of hBMP-7 gene transfected bone marrow stromal cells (BMSC) and to provide a new way for repairing the periodontal defects.


METHODS
A total of 30 experimental class II furcation defects in five Beagle dogs were created surgically. The transfected and non-transfected BMSC were seeded in collagen membrane at an initial concentration of 1 x 10(7)/ml and transplanted into experimental class II furcation defects. The animals were sacrificed at 12 weeks post-operation and periodontal regeneration was evaluated by the histological and morphometric analysis.


RESULTS
In both experimental and control groups, there were different extents of regeneration. The percentage of new alveolar area in transfected and non-transfected BMSC were significantly higher than the control (P < 0.05). And there was also significant difference between two experimental groups (P < 0.05). The percentage of new cementum length in two experimental groups were significantly higher than the control (P < 0.05). But there was no significant difference between two BMSCs groups (P > 0.05).


CONCLUSIONS
hBMP-7 gene enhanced tissue engineering could be a better way to repair the periodontal defects.
Li
Yan-Fen
YF
Department of Periodontology, Affiliate Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China.
Yan
Fu-Hua
FH
Zhong
Quan
Q
Zhao
Xin
X
D000818:Animals; D001854:Bone Marrow Cells; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics*; D004285:Dogs; D015316:Genetic Therapy / Q000379:methods; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D059630:Mesenchymal Stem Cells; D010510:Periodontal Diseases / Q000628:therapy; D023822:Tissue Engineering / Q000379:methods; D014162:Transfection
D055419:Bone Morphogenetic Protein 7