High-sensitivity cerebral perfusion mapping in mice by kbGRASE-FAIR at 9.4 T.
The combination of flow-sensitive alternating inversion recovery (FAIR) and single-shot k-space-banded gradient- and spin-echo (kbGRASE) is proposed here to measure perfusion in the mouse brain with high sensitivity and stability. Signal-to-noise ratio (SNR) analysis showed that kbGRASE-FAIR boosts image and temporal SNRs by 2.01 ± 0.08 and 2.50 ± 0.07 times, respectively, when compared with standard single-shot echo planar imaging (EPI)-FAIR implemented in our experimental systems, although the practically achievable spatial resolution was slightly reduced. The effects of varying physiological parameters on the precision and reproducibility of cerebral blood flow (CBF) measurements were studied following changes in anesthesia regime, capnia and body temperature. The functional MRI time courses with kbGRASE-FAIR showed a more stable response to 5% CO(2) than did those with EPI-FAIR. The results establish kbGRASE-FAIR as a practical and robust protocol for quantitative CBF measurements in mice at 9.4 T.
Zheng
Bingwen
B
Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Biopolis, Singapore. zheng_bingwen@sbic.a-star.edu.sg
Lee
Philip Teck Hock
PT
Golay
Xavier
X
D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000502:physiology; D001931:Brain Mapping / Q000379:methods*; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D017352:Echo-Planar Imaging / Q000379:methods*; D004558:Electric Stimulation; D006439:Hemodynamics; D006614:Hindlimb / Q000294:innervation; D006801:Humans; D006935:Hypercapnia / Q000378:metabolism; D007035:Hypothermia; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007530:Isoflurane / Q000494:pharmacology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012680:Sensitivity and Specificity; D013113:Spin Labels
D018685:Anesthetics, Inhalation; D013113:Spin Labels; D007530:Isoflurane