title: Cell type-specific expression of adenomatous polyposis coli in lung development, injury, and repair.
abstract: Adenomatous polyposis coli (Apc) is critical for Wnt signaling and cell migration. The current study examined Apc expression during lung development, injury, and repair. Apc was first detectable in smooth muscle layers in early lung morphogenesis, and was highly expressed in ciliated and neuroendocrine cells in the advanced stages. No Apc immunoreactivity was detected in Clara or basal cells, which function as stem/progenitor cell in adult lung. In ciliated cells, Apc is associated mainly with apical cytoplasmic domain. In response to naphthalene-induced injury, Apc(positive) cells underwent squamous metaplasia, accompanied by changes in Apc subcellular distribution. In conclusion, both spatial and temporal expression of Apc is dynamically regulated during lung development and injury repair. Differential expression of Apc in progenitor vs. nonprogenitor cells suggests a functional role in cell-type specification. Subcellular localization changes of Apc in response to naphthalene injury suggest a role in cell shape and cell migration.
authors.affiliation: Department of Pediatrics, Division of Neonatology, Women's & Children's Hospital, USC Keck School of Medicine, Los Angeles, California 90033, USA.
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
authors.forename: Yiming
authors.initials: Y
authors.lastname: Xing
authors.forename: Belinda
authors.initials: B
authors.lastname: Chan
authors.forename: Nora
authors.initials: N
authors.lastname: Heisterkamp
authors.forename: John
authors.initials: J
authors.lastname: Groffen
authors.forename: Zea
authors.initials: Z
authors.lastname: Borok
authors.forename: Parviz
authors.initials: P
authors.lastname: Minoo
authors.forename: Changgong
authors.initials: C
authors.lastname: Li
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000032:analysis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D008168:Lung / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology; D055370:Lung Injury / Q000139:chemically induced; D009281:Naphthalenes / Q000494:pharmacology; D012038:Regeneration / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism; D014018:Tissue Distribution; D014945:Wound Healing / Q000235:genetics
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D009281:Naphthalenes; C031721:naphthalene