title: Hyperpolarization of human mesenchymal stem cells in response to magnetic force.
abstract: Magnetic particle tagging techniques are currently being applied to tissue engineering applications such as controlled differentiation of mesenchymal stem cells (MSC). In order to define key mechanotransducers underpinning these applications, the electrophysiological responses of human MSCs (hMSC) have been investigated. Ferromagnetic microparticles were coated with L-arginyl-glycyl-L-aspartic acid in order to target the application of dynamic force (6 pN) directly to cell surface integrins. Human MSCs demonstrated cell membrane hyperpolarization responses after the application of force, mediated by BK channels and intracellular calcium release.
authors.affiliation: Institute of Science and Technology in Medicine, University of Keele, Stoke-on-Trent, ST4 7QB, UK. glen.kirkham@nottingham.ac.uk
authors.forename: Glen R
authors.initials: GR
authors.lastname: Kirkham
authors.forename: Kerry J
authors.initials: KJ
authors.lastname: Elliot
authors.forename: Ahmed
authors.initials: A
authors.lastname: Keramane
authors.forename: Donald M
authors.initials: DM
authors.lastname: Salter
authors.forename: Jon P
authors.initials: JP
authors.lastname: Dobson
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: El Haj
authors.forename: Sarah Harriet
authors.initials: SH
authors.lastname: Cartmell
mesh_terms: D000704:Analysis of Variance; D015220:Calcium Channels; D016764:Cell Polarity / Q000502:physiology* / Q000528:radiation effects; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D004594:Electrophysiology / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009543:Nifedipine / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D005290:Ferric Compounds; D051036:Large-Conductance Calcium-Activated Potassium Channels; C001215:ferrite; D019284:Thapsigargin; D009543:Nifedipine