title: Prevention of filtering surgery failure by subconjunctival injection of a novel peptide hydrogel into rabbit eyes.
abstract: A novel biocompatible hydrogel was prepared based on the supramolecular self-assembly of a peptide containing a bioactive RGD (arginine-glycine-aspartic acid) sequence and a hydrophobic N-fluorenyl-9-methoxycarbonyl (FMOC) tail. When the self-assembled peptide hydrogel was administered after the filtering surgery of rabbit eyes, the level of connective tissue growth factor (CTGF) mRNA as well as the mean intraocular pressure (IOP) was significantly lower than that of the control eyes during the 21 postoperative days. The filtration bleb and ultrasound biomicroscopy (UBM) images showed that a patent bleb and a filtration fistula could be found in the surgical site of a rabbit eye during the whole experimental period. Histological analysis further evidenced that the filtering surgical wound healing was a normal healing process without scar formation. This new approach, making use of a self-assembled peptide hydrogel to normalize filtering surgical wound healing, may have potential for glaucoma filtering surgery.
authors.affiliation: Department of Ophthalmology, The Central Hospital of Wuhan, Wuhan 430014, People's Republic of China. niuniu419519@163.com
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Xiao-Ding
authors.initials: XD
authors.lastname: Xu
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Feng
authors.forename: Chong
authors.initials: C
authors.lastname: Peng
authors.forename: Fa-Gang
authors.initials: FG
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002921:Cicatrix / Q000209:etiology* / Q000517:prevention & control*; D003316:Corneal Diseases / Q000209:etiology* / Q000517:prevention & control*; D018463:Filtering Surgery / Q000009:adverse effects*; D020100:Hydrogels / Q000008:administration & dosage*; D056965:Injections, Intraocular; D008422:Materials Testing; D009842:Oligopeptides / Q000008:administration & dosage*; D011817:Rabbits; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
chemical_list: D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid