Growth inhibition of Microcystis aeruginosa by white-rot fungus Lopharia spadicea.
Harmful cyanobacterial blooms cause water deterioration and threaten human health. It is necessary to remove harmful cyanobacteria with useful methods. A bio-treatment may be one of the best ways to do this. A strain of specific white-rot fungus, Lopharia spadicea, with algicidal ability was isolated. Its algicidal ability on algae under various conditions was determined using three main influence factors: initial chlorophyll-a content, initial pH, and algal cell mixture. The result showed that the chlorophyll-a content of Microcystis aeruginosa FACHB-912, Oocystis borgei FACHB-1108, and Microcystis flos-aquae FACHB-1028 decreased from 798+/-13, 756+/-40, and 773+/-24 microg/L to 0 within 39 h. L. spadicea could also remove more than 95% chlorophyll-a when initial chlorophyll-a content increased from 397+/-13 to 2,132+/-4 microg/L. Moreover, the strain has great removal ability under a broad initial pH range of 5.5 to 9.5. The chlorophyll-a content of the three algal strain mixtures decreased from about 672+/-23 microg/L to 0 within 45 h. After superoxide dismutase (SOD) and malondialdehyde (MAD) were assessed in a co-culture of L. spadicea, it was observed that an increase in MAD content was correlated with the decrease in chlorophyll-a content of M. aeruginosa FACHB-912. This result suggested that the algae was not only greatly inhibited but also severely damaged by the fungus.
Wang
Q
Q
School of life science, Nanjing University, 21 Hankou Road, Nanjing Jiangsu Province 210093, China.
Su
M
M
Zhu
W
W
Li
X
X
Jia
Y
Y
Guo
P
P
Chen
Z
Z
Jiang
W
W
Tian
X
X
D001487:Basidiomycota / Q000502:physiology*; D018533:Biomass; D016923:Cell Death; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation; D008315:Malondialdehyde; D046931:Microcystis / Q000166:cytology* / Q000254:growth & development*; D010572:Pest Control, Biological; D013482:Superoxide Dismutase; D013997:Time Factors
D002734:Chlorophyll; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000077194:Chlorophyll A