title: Mechanism of action of tin-containing fluoride solutions as anti-erosive agents in dentine - an in vitro tin-uptake, tissue loss, and scanning electron microscopy study.
abstract: Solutions containing tin and fluoride exhibit remarkable anti-erosive properties with tin ions as a major agent. To elucidate its mechanism of action in dentine, the tin uptake on and in the tissue was investigated and related to histological findings and substance loss. Samples were treated twice daily, each treatment lasting for 2 min, with fluoride solutions [pH 4.5; 1,500 parts per million (p.p.m.) F] containing 2,100, 1,400, or 400 p.p.m. Sn as SnCl(2). In experiments 1 and 2, samples were eroded with citric acid (pH 2.3) six times each day, each treatment lasting for 5 min; in experiment 2, the demineralized organic matrix was continuously digested by collagenase; in experiment 3, no erosive challenges were performed. Sample surfaces and cross-sections were investigated using energy dispersive X-ray spectroscopy, scanning electron microscopy, and profilometry. Surface retention of tin was found in almost all treatment groups and was highest in experiment 2. On cross-sections, tin was retained within the organic matrix; in mineralized areas, tin was found mainly within a depth of 10 mum. Test solutions inhibited substance loss significantly; in experiment 2, the effect was dose-dependent. Erosion inhibition seemed to depend mainly on the incorporation of tin in the mineralized dentine when the organic portion was preserved, but on surface precipitation when the organic portion was continuously digested.
authors.affiliation: Department of Conservative and Preventive Dentistry, Dental Clinic, Justus Liebig University Giessen, Giessen, Germany. carolina.ganss@dentist.med.uni-giessen.de
authors.forename: Carolina
authors.initials: C
authors.lastname: Ganss
authors.forename: Martin
authors.initials: M
authors.lastname: Hardt
authors.forename: Adrian
authors.initials: A
authors.lastname: Lussi
authors.forename: Ann-Kristin
authors.initials: AK
authors.lastname: Cocks
authors.forename: Joachim
authors.initials: J
authors.lastname: Klimek
authors.forename: Nadine
authors.initials: N
authors.lastname: Schlueter
mesh_terms: D011232:Chemical Precipitation; D019343:Citric Acid / Q000009:adverse effects; D017364:Collagenases / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D003959:Diamines / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008855:Microscopy, Electron, Scanning; D012969:Sodium Fluoride / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors; D014001:Tin / Q000493:pharmacokinetics / Q000494:pharmacology; D014002:Tin Fluorides / Q000493:pharmacokinetics / Q000494:pharmacology*; D017001:Tooth Demineralization / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D014077:Tooth Erosion / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014089:Tooth Remineralization
chemical_list: D003959:Diamines; D014002:Tin Fluorides; D019343:Citric Acid; D014001:Tin; C495399:Olaflur; D012969:Sodium Fluoride; D017364:Collagenases; D005459:Fluorides