Both forward and reverse TCA cycles operate in green sulfur bacteria.
The anoxygenic green sulfur bacteria (GSBs) assimilate CO(2) autotrophically through the reductive (reverse) tricarboxylic acid (RTCA) cycle. Some organic carbon sources, such as acetate and pyruvate, can be assimilated during the phototrophic growth of the GSBs, in the presence of CO(2) or HCO(3)(-). It has not been established why the inorganic carbonis required for incorporating organic carbon for growth and how the organic carbons are assimilated. In this report, we probed carbon flux during autotrophic and mixotrophic growth of the GSB Chlorobaculum tepidum. Our data indicate the following: (a) the RTCA cycle is active during autotrophic and mixotrophic growth; (b) the flux from pyruvate to acetyl-CoA is very low and acetyl-CoA is synthesized through the RTCA cycle and acetate assimilation; (c) pyruvate is largely assimilated through the RTCA cycle; and (d) acetate can be assimilated via both of the RTCA as well as the oxidative (forward) TCA (OTCA) cycle. The OTCA cycle revealed herein may explain better cell growth during mixotrophic growth with acetate, as energy is generated through the OTCA cycle. Furthermore, the genes specific for the OTCA cycle are either absent or down-regulated during phototrophic growth, implying that the OTCA cycle is not complete, and CO(2) is required for the RTCA cycle to produce metabolites in the TCA cycle. Moreover, CO(2) is essential for assimilating acetate and pyruvate through the CO(2)-anaplerotic pathway and pyruvate synthesis from acetyl-CoA.
Tang
Kuo-Hsiang
KH
Department of Biology and Chemistry, Washington University, St Louis, Missouri 63130, USA.
Blankenship
Robert E
RE
D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics / Q000378:metabolism; D000084:Acetate Kinase / Q000235:genetics / Q000378:metabolism; D000085:Acetates / Q000737:chemistry / Q000378:metabolism; D000105:Acetyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D052818:Autotrophic Processes; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D002247:Carbon Isotopes; D019414:Chlorobi / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002952:Citric Acid Cycle; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D015394:Molecular Structure; D019289:Pyruvic Acid / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000085:Acetates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D019344:Lactic Acid; D000105:Acetyl Coenzyme A; D002244:Carbon; D019289:Pyruvic Acid; D001275:ATP Citrate (pro-S)-Lyase; D000084:Acetate Kinase; D003066:Coenzyme A Ligases; C101958:acetate-CoA ligase (ADP-forming)