title: Crystallography of membrane proteins: from crystallization to structure.
abstract: Although crystallographic studies of membrane proteins have progressed in the last 5 years, the field still remains challenging with several severe bottlenecks. The chapter focuses on the crystallization and describes two approaches, the classical vapor diffusion method and the more recent use of lipidic phases. General aspects on the crystallization principles as well as more practical details are given. In a more synthetic way, the chapter also addresses how structures are solved by X-ray crystallography, and highlights aspects that are specific to membrane proteins.
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, B.P. 181, Grenoble, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Deniaud
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Moiseeva
authors.forename: Valentin
authors.initials: V
authors.lastname: Gordeliy
authors.forename: Eva
authors.initials: E
authors.lastname: Pebay-Peyroula
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008962:Models, Theoretical; D017433:Protein Structure, Secondary
chemical_list: D008565:Membrane Proteins