ProstaglandinE(2) Release, Squalene Monohydroperoxide Production and Cell Toxicity of Skin((2TM)) ZK1301 as a Human Skin Model in the Presence of Haematoporphylin and Ultraviolet-A Irradiation.
For the clarification of the mechanism of ultraviolet-A (UVA)-induced cytotoxicity to skin, the prostaglandinE(2) (PGE(2)) release and squalene monohydroperoxide (SQOOH) production in a human skin model (Skin(2TM) ZK1301) in the presence of haematoporphylin (HP) as a photosensitizer were investigated.The PGE(2) release and SQOOH production were significantly increased depending on both the irradiation time of UVA (350-380nm) and the HP concentration. In addition, concentration-dependent inhibitions of cytotoxicity, PGE(2) release and SQOOH production were observed when a UVA sunscreen was applied to the surface of Skin(2TM). These results suggest that the mediator of inflammation and lipid hydroperoxide production induced by UVA-HP photosensitization cause cytotoxicity to skin, and that the test method of UVA-HP photosensitization using the Skin(2TM) ZK1301 model is a useful in vitro model for studying UVA phototoxicity and for UVA sunscreen evaluations.
Uchino
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T
Division of Environmental Chemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Tokunaga
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H
Ando
M
M