Patient factors affecting culture of Helicobacter pylori isolated from gastric mucosal specimens.
OBJECTIVE
Culture is one of the methods used for detecting Helicobacter pylori in the stomach. However, since it is costly, labor-consuming, and in a number of infected subjects gives a false negative result, the procedure is not routinely used. The aim of the study was to analyze some of the factors that may affect the outcome of H. pylori culture from endoscopic gastric mucosal specimens.


METHODS
The study was conducted in a group of 265 subjects. The culture of gastric mucosal specimens was verified by urease test and histological examination. If the culture result was not consistent with one or two verifying tests, an additional two tests were used, i.e. H. pylori antigens in stool samples and anti-H. pylori antibodies in blood serum.


RESULTS
In patients infected with H. pylori (at least two positive diagnostic tests), the analysis of factors that may affect the culture outcome revealed that neither age, gender, smoking, history of eradication, endoscopic diagnosis, use of proton pump inhibitors, ultrasonography of the abdomen or chest radiology performed the day before or on the day of gastroscopy, nor preparation for colonoscopy using osmotic fluids 1-2 days prior to gastroscopy had an effect on the culture outcome. Only high activity of gastritis (neutrophil infiltration) and low bacterial load in gastric mucosal specimens as well as drinking alcohol and the use of histamine H₂ receptor blockers reduced culture efficacy in infected subjects.


CONCLUSIONS
High activity of gastritis, low bacterial load, drinking alcohol and the use of histamine H₂ receptor blockers can be the cause of failed H. pylori culture from gastric mucosa in the infected subjects. These factors should be taken into consideration when qualifying patients for the test and interpreting the results.
Leszczyńska
K
K
Department of Diagnostic Microbiology, Medical University of Białystok, Poland.
Namiot
A
A
Namiot
Z
Z
Leszczyńska
J K
JK
Jakoniuk
P
P
Chilewicz
M
M
Namiot
D B
DB
Kemona
A
A
Milewski
R
R
Bucki
R
R
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis; D016099:Endoscopy, Gastrointestinal; D005243:Feces / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology / Q000503:physiopathology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial