The A-repeat links ASF/SF2-dependent Xist RNA processing with random choice during X inactivation.
One X chromosome, selected at random, is silenced in each female mammalian cell. Xist encodes a noncoding RNA that influences the probability that the cis-linked X chromosome will be silenced. We found that the A-repeat, a highly conserved element within Xist, is required for the accumulation of spliced Xist RNA. In addition, the A-repeat is necessary for X-inactivation to occur randomly. In combination, our data suggest that normal Xist RNA processing is important in the regulation of random X-inactivation. We propose that modulation of Xist RNA processing may be part of the stochastic process that determines which X chromosome will be inactivated.
Royce-Tolland
Morgan E
ME
Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA.
Andersen
Angela A
AA
Koyfman
Hannah R
HR
Talbot
Dale J
DJ
Wutz
Anton
A
Tonks
Ian D
ID
Kay
Graham F
GF
Panning
Barbara
B
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012323:RNA Processing, Post-Transcriptional; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics; D000068103:Serine-Arginine Splicing Factors; D049951:X Chromosome Inactivation / Q000235:genetics*
D006657:Histones; D009687:Nuclear Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; C077885:XIST non-coding RNA; D000068103:Serine-Arginine Splicing Factors