title: Probing protease activity by single-fluorescent-protein nanocapsules.
abstract: We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles (UCLA), Los Angeles, California 90095, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gu
authors.forename: Anuradha
authors.initials: A
authors.lastname: Biswas
authors.forename: Kye-Il
authors.initials: KI
authors.lastname: Joo
authors.forename: Biliang
authors.initials: B
authors.lastname: Hu
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D053769:Nanocapsules; D010447:Peptide Hydrolases