Expression of liver X receptors in pregnancies complicated by preeclampsia.
Preeclampsia is a pregnancy-specific disorder associated with hyperlipidemia. Liver X receptor (LXR) alpha and LXRbeta are key regulators of lipid homeostasis. In the current study, we investigated expression of LXRalpha, LXRbeta and their target genes in human term placenta, decidua and subcutaneous adipose tissue from pregnancies complicated by preeclampsia. Furthermore, we analyzed the protein levels of LXRalpha and LXRbeta in placenta. We also analyzed lipid concentrations in term placental tissue. Gene expression of LXRalpha, LXRbeta and fatty acid transporter CD36 was significantly decreased in placental tissues, while increased expression was observed for LXRalpha in adipose tissue, from pregnancies complicated by preeclampsia. The placental protein level of LXRbeta was reduced, and there was a positive correlation between placental LXRbeta mRNA expression and placental free fatty acids in preeclampsia. Our results suggest a possible role for LXRbeta as a transcriptional regulator in preeclampsia.
Weedon-Fekjær
M S
MS
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway. susannew@basalmed.uio.no
Johnsen
G M
GM
Anthonisen
E H
EH
Sugulle
M
M
Nebb
H I
HI
Duttaroy
A K
AK
Staff
A C
AC
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D018955:CD36 Antigens / Q000096:biosynthesis; D003656:Decidua / Q000378:metabolism; D005260:Female; D006801:Humans; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000096:biosynthesis*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
D018955:CD36 Antigens; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; D057093:Orphan Nuclear Receptors