Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
Highly differentiated normal human hepatocytes represent the gold standard cellular model for basic and applied research in liver physiopathology, pharmacology, toxicology, virology, and liver biotherapy. Nowadays, although livers from organ donors or medically required resections represent the current sources of hepatocytes, the possibility to generate hepatocytes from the differentiation of adult and embryonic stem cells represents a promising opportunity. The aim of this chapter is to describe our experience with the isolation from adult human liver and culture of non-parenchymal epithelial cells. Under appropriate conditions, these cells differentiate in vitro in hepatocyte-like cells and therefore appear to behave as liver progenitor cells.
Gerbal-Chaloin
Sabine
S
INSERM U632, Hepatic Physiopathology, Montpellier, France.
Duret
Cédric
C
Raulet
Edith
E
Navarro
Francis
F
Blanc
Pierre
P
Ramos
Jeanne
J
Maurel
Patrick
P
Daujat-Chavanieu
Martine
M
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism