Expression and purification of Z protein from Junín virus.
Arenaviridae comprises 23 recognized virus species with a bipartite ssRNA genome and an ambisense coding strategy. The virions are enveloped and include nonequimolar amounts of each genomic RNA species, designated L and S, coding for four ORFs (N, GPC, L, and Z). The arenavirus Junín (JUNV) is the etiological agent of Argentine Hemorrhagic Fever, an acute disease with high mortality rate. It has been proposed that Z is the functional counterpart of the matrix proteins found in other negative-stranded enveloped RNA viruses. Here we report the optimized expression of a synthetic gene of Z protein, using three expression systems (two bacterial and a baculoviral one). One of these recombinant proteins was used to generate antibodies. A bioinformatic analysis was made where Z was subdivided into three domains. The data presented contributes methodologies for Z recombinant production and provides the basis for the development of new experiments to test its function.
Goñi
S E
SE
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, LIGBCM, Roque Sáenz Peña 352, CP B1876BXD, Bernal, Buenos Aires, Argentina. sgoni@unq.edu.ar
Borio
C S
CS
Romano
F B
FB
Rota
R P
RP
Pilloff
M G
MG
Iserte
J A
JA
Tortorici
M A
MA
Stephan
B I
BI
Bilen
M F
MF
Ghiringhelli
P D
PD
Lozano
M E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D001117:Arenaviridae Infections / Q000821:virology; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D018051:Junin virus / Q000235:genetics*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D018411:Spodoptera / Q000235:genetics; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins