title: Neurogenic marker abnormalities in the hippocampus in dementia with Lewy bodies.
abstract: Dementia with Lewy bodies (DLB) is associated with alpha synuclein pathology and slowly progressive dementia. Progenitor abnormalities have previously been reported in the subventricular zone (SVZ) adjacent to the lateral ventricle. To evaluate changes in neural stem cells and progenitors in the hippocampal neurogenic niche, immunohistochemistry (IHC) using the neural stem cell markers Musashi 1, nestin, proliferating cell nuclear antigen (PCNA), doublecortin, and glial fibrillary acidic protein (GFAP) were examined in age-matched control and DLB groups. Staining was quantified in the hippocampal SVZ, subgranular layer (SGL) and ependymal cell layer (EPL). There was a significant loss in DLB of Musashi 1 (P < 0.01) in all areas, an increase in PCNA in hippocampal SVZ (P = 0.01) and SGL (P = 0.05), and an increase in doublecortin in the hippocampal SVZ (P = 0.04) and EPL (P = 0.02). This is the first report of the changes in neurogenic markers in the hippocampal SVZ and EPL in DLB and may offer the potential for understanding disease pathology and in the devising of treatment.
authors.affiliation: Institute for Ageing and Health, Campus for Ageing and Vitality, Newcastle University, Newcastle Upon Tyne, United Kingdom. mary.johnson@ncl.ac.uk
authors.forename: Mary
authors.initials: M
authors.lastname: Johnson
authors.forename: Antigoni
authors.initials: A
authors.lastname: Ekonomou
authors.forename: Carl
authors.initials: C
authors.lastname: Hobbs
authors.forename: Clive G
authors.initials: CG
authors.lastname: Ballard
authors.forename: Robert H
authors.initials: RH
authors.lastname: Perry
authors.forename: Elaine K
authors.initials: EK
authors.lastname: Perry
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D002923:Cilia / Q000378:metabolism / Q000473:pathology; D000089622:Doublecortin Domain Proteins; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology / Q000378:metabolism; D016631:Lewy Bodies / Q000378:metabolism*; D020961:Lewy Body Disease / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D058953:Neural Stem Cells / Q000378:metabolism / Q000473:pathology; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D009479:Neuropeptides / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012189:Retrospective Studies; D014015:Tissue Banks; D051844:alpha-Synuclein / Q000378:metabolism
chemical_list: D000089622:Doublecortin Domain Proteins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; C491297:MSI1 protein, human; D008869:Microtubule-Associated Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D009479:Neuropeptides; D018809:Proliferating Cell Nuclear Antigen; D016601:RNA-Binding Proteins; D051844:alpha-Synuclein