genotoxic effects induced by beta-oestradiol in vitro.
The carcinogenic endogenous hormone beta-oestradiol induces genotoxic effects in short-term mammalian tests. In this study we investigated micronucleus induction in V79 cells. The early induction of primarily kinetochore positive micronuclei indicated a mechanism involving mitotic disturbances. Metaphase arrangements that showed displaced chromosomes suggested that these chromosomes may be enclosed in micronuclei. However, observation of live cells revealed that most of the displaced chromosomes reintegrated. In some of these cells chromatid separation at anaphase was impaired and a small chromatin fragment was separated from the remaining chromatin.
Eckert
I
I
Department of Toxicology, University of Würzburg, Versbacher Str. 9, 97078, Würzburg, Germany.
Stopper
H
H