title: Comment on "Increased MKK4 abundance with replicative senescence is linked to the joint reduction of multiple microRNAs".
abstract: Marasa et al. (Research Article, 27 October 2009, DOI: 10.1126/scisignal.2000442) reported that the human kinase p38-regulated/activated protein kinase (PRAK) was phosphorylated on residue Ser(93) in senescent cells. We have been unable to detect phosphorylation at this site with the antibody that they used, and the commercial supplier of this antibody has discontinued its availability, which casts doubt on whether this residue of PRAK is phosphorylated.
authors.forename: Ugo
authors.initials: U
authors.lastname: Moens
authors.forename: Alexey
authors.initials: A
authors.lastname: Shiryaev
authors.forename: Gianina
authors.initials: G
authors.lastname: Dumitriu
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D001709:Biotechnology; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D000906:Antibodies; D047908:Intracellular Signaling Peptides and Proteins; D035683:MicroRNAs; C111049:MAP-kinase-activated kinase 5; D017346:Protein Serine-Threonine Kinases; D048670:MAP Kinase Kinase 4