Caffeine, selected metabolic gene variants, and risk for neural tube defects.
BACKGROUND
Investigations of maternal caffeine intake and neural tube defects (NTDs) have not considered genetic influences. Caffeine metabolism gene effects were examined in the National Birth Defects Prevention Study.


METHODS
Average daily caffeine was summed from self-reported coffee, tea, soda, and chocolate intake for mothers of 768 NTD cases, and 4143 controls delivered from 1997 to 2002. A subset of 306 NTD and 669 control infants and their parents were genotyped for CYP1A2*1F, NAT2 481C>T, and NAT2 590G>A. CYP1A2*1F was classified by fast or slow oxidation status, and NAT2 variants were categorized into rapid or slow acetylation status. Case-control logistic regression analyses, family-based transmission/disequilibrium tests and log-linear analyses, and hybrid log-linear analyses were conducted to produce odds ratios (ORs) or relative risks (RRs) and 95% confidence intervals (CIs) for caffeine intake and maternal and infant gene variants, and to examine interaction effects.


RESULTS
NTDs were independently associated with infant slow NAT2 acetylator status (RR, 2.00; 95% CI, 1.10-3.64) and maternal CYP1A2*1F fast oxidation status (OR, 1.49; 95% CI, 1.10-2.03). Mothers who consumed caffeine, oxidized CYP1A2*1F quickly, and acetylized NAT2 slowly had a nonsignificantly elevated estimated risk for an NTD-affected pregnancy (OR, 3.10; 95% CI, 0.86-11.21). Multiplicative interaction effects were observed between maternal caffeine and infant CYP1A2*1F fast oxidizer status (p(interaction) = 0.03).


CONCLUSIONS
The association identified between maternal CYP1A2*1F fast oxidation status and NTDs should be examined further in the context of the other substrates of CYP1A2. Maternal caffeine and its metabolites may be associated with increased risk for NTD-affected pregnancies in genetically susceptible subgroups.
Schmidt
Rebecca J
RJ
Department of Public Health Sciences, University of California, Davis, 95616, USA. rjschmidt@ucdavis.edu
Romitti
Paul A
PA
Burns
Trudy L
TL
Murray
Jeffrey C
JC
Browne
Marilyn L
ML
Druschel
Charlotte M
CM
Olney
Richard S
RS
D000107:Acetylation; D000293:Adolescent; D000328:Adult; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D002110:Caffeine / Q000009:adverse effects*; D016022:Case-Control Studies; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D016015:Logistic Models; D018811:Maternal Exposure; D009436:Neural Tube Defects / Q000139:chemically induced / Q000235:genetics*; D016017:Odds Ratio; D010084:Oxidation-Reduction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011247:Pregnancy; D018570:Risk Assessment
D002110:Caffeine; C504573:CYP1A2 protein, human; D019388:Cytochrome P-450 CYP1A2; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human