title: The ability of the BANA Test to detect different levels of P. gingivalis, T. denticola and T. forsythia.
abstract: The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.
authors.affiliation: Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
authors.forename: José Alexandre de
authors.initials: JA
authors.lastname: Andrade
authors.forename: Magda
authors.initials: M
authors.lastname: Feres
authors.forename: Luciene Cristina de
authors.initials: LC
authors.lastname: Figueiredo
authors.forename: Sérgio Luiz
authors.initials: SL
authors.lastname: Salvador
authors.forename: Sheila Cavalca
authors.initials: SC
authors.lastname: Cortelli
mesh_terms: D000328:Adult; D001587:Benzoylarginine-2-Naphthylamide; D016009:Chi-Square Distribution; D055113:Chronic Periodontitis / Q000175:diagnosis / Q000382:microbiology; D015169:Colony Count, Microbial; D015342:DNA Probes; D003753:Dental Health Surveys; D003773:Dental Plaque / Q000382:microbiology; D057075:Enzyme Assays; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D010510:Periodontal Diseases / Q000175:diagnosis* / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D048373:Treponema denticola / Q000302:isolation & purification*
chemical_list: D015342:DNA Probes; D001587:Benzoylarginine-2-Naphthylamide