Analysis of antimicrobial peptides from porcine neutrophils.
Cationic host defence peptides are important components of innate immunity in pigs and other mammalians. Most of these peptides have a direct antimicrobial activity and they also have a broad spectrum of effects on the host immune system, which may be taken into account in the introduction of novel therapeutics. Our method permits simultaneous isolation of six antibacterial peptides, i.e. prophenin-1, prophenin-2, PR-39, and protegrins 1-3 from a porcine neutrophil crude extract and characterisation of them. Among the obtained peptides the greatest bactericidal activity expressed as MBC was seen in protegrins (10 μg/ml), whereas in the other studied peptides MBC was on the level of 20 μg/ml. Minimal inhibitory concentrations (MIC) reached 10 μg/ml for protegrins 1-3 and 20 μg/ml for prophenins, and PR-39. Within the bactericidal range all isolated peptides didn't show cytotoxicity on cell lines used in our experiment.
Wessely-Szponder
Joanna
J
Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland. joanna.wessely@up.lublin.pl
Majer-Dziedzic
Barbara
B
Smolira
Anna
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D002853:Chromatography, Liquid / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine
D000900:Anti-Bacterial Agents; D010455:Peptides