title: The ABC transporter AnrAB contributes to the innate resistance of Listeria monocytogenes to nisin, bacitracin, and various beta-lactam antibiotics.
abstract: A mariner transposon bank was used to identify loci that contribute to the innate resistance of Listeria monocytogenes to the lantibiotic nisin. In addition to highlighting the importance of a number of loci previously associated with nisin resistance (mprF, virRS, and telA), a nisin-sensitive phenotype was associated with the disruption of anrB (lmo2115), a gene encoding the permease component of an ABC transporter. The contribution of anrB to nisin resistance was confirmed by the creation of nonpolar deletion mutants. The loss of this putative multidrug resistance transporter also greatly enhanced sensitivity to bacitracin, gallidermin, and a selection of β-lactam antibiotics. A comparison of the relative antimicrobial sensitivities of a number of mutants established the ΔanrB strain as being one of the most bacitracin-sensitive L. monocytogenes strains identified to date.
authors.affiliation: Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Barry
authors.initials: B
authors.lastname: Collins
authors.forename: Nicola
authors.initials: N
authors.lastname: Curtis
authors.forename: Paul D
authors.initials: PD
authors.lastname: Cotter
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D009561:Nisin / Q000494:pharmacology*; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001414:Bacitracin; D009561:Nisin