title: Relaxant effect of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated rat ileum contractions.
abstract: Helichrysum plicatum (Turkish Helichrysum) has been used in folk medicine for the treatment of gastric and hepatic disorders. The aim of the present study was to examine the relaxant activity of an extract of H. plicatum flowers on isolated rat ileum. Segments of ileum of rats were suspended in an organ bath. Cumulative concentrations of H. plicatum ethanol extract induced a relaxant effect on spontaneous rat ileum contractions. H. plicatum extract caused a mean contractile response of 81.68 +/- 6.17% (at a dose of 0.01 mg/mL) and 30.08 +/- 9.07% (at a dose of 1 mg/mL). A similar effect was observed with papaverine (0.01-3 microg/mL). H. plicatum extract (0.01-1 mg/mL) relaxed high K+ (80 mM) precontractions, an effect similar to that caused by papaverine (0.01-3 microg/mL). The plant extract (0.03-0.3 mg/mL) also induced a significant depression of the cumulative concentration response curve for acetylcholine (5-1500 nM) (p < 0.01). Atropine (140 nM) abolished the acetylcholine effect. The extract (0.03-0.3 mg/mL) reduced the histamine (1-300 nM) and BaCl2 (3-900 microM) induced contractions (p < 0.01). Our results showed the relaxant effect of the ethanol extract of Helichrysum plicatum flowers on the isolated rat intestine Extract of H. plicatum can inhibit the spontaneous ileum contractions and contractions induced by acetylcholine, histamine, barium and potassium ions.
authors.affiliation: Institute for Medicinal Plants Research, Tadeusa Kosćuska 1, 11000 Belgrade, Serbia. dbigovic@mocbilja.rs
authors.forename: Dubravka
authors.initials: D
authors.lastname: Bigovic
authors.forename: Suzana
authors.initials: S
authors.lastname: Brankovic
authors.forename: Dusanka
authors.initials: D
authors.lastname: Kitic
authors.forename: Mirjana
authors.initials: M
authors.lastname: Radenkovic
authors.forename: Teodora
authors.initials: T
authors.lastname: Jankovic
authors.forename: Katarina
authors.initials: K
authors.lastname: Savikin
authors.forename: Slavoljub
authors.initials: S
authors.lastname: Zivanovic
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000431:Ethanol; D036562:Helichrysum / Q000737:chemistry*; D006632:Histamine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats
chemical_list: D010936:Plant Extracts; D000431:Ethanol; D006632:Histamine; D000109:Acetylcholine