title: Electrospun poly(D/L-lactide-co-L-lactide) hybrid matrix: a novel scaffold material for soft tissue engineering.
abstract: Electrospinning is a long-known polymer processing technique that has received more interest and attention in recent years due to its versatility and potential use in the field of biomedical research. The fabrication of three-dimensional (3D) electrospun matrices for drug delivery and tissue engineering is of particular interest. In the present study, we identified optimal conditions to generate novel electrospun polymeric scaffolds composed of poly-D/L-lactide and poly-L-lactide in the ratio 50:50. Scanning electron microscopic analyses revealed that the generated poly(D/L-lactide-co-L-lactide) electrospun hybrid microfibers possessed a unique porous high surface area mimicking native extracellular matrix (ECM). To assess cytocompatibility, we isolated dermal fibroblasts from human skin biopsies. After 5 days of in vitro culture, the fibroblasts adhered, migrated and proliferated on the newly created 3D scaffolds. Our data demonstrate the applicability of electrospun poly(D/L-lactide-co-L-lactide) scaffolds to serve as substrates for regenerative medicine applications with special focus on skin tissue engineering.
authors.affiliation: Fraunhofer-Institute for Interfacial Engineering and Biotechnology (IGB), Nobelstrasse 12, 70569, Stuttgart, Germany.
authors.forename: Petra J
authors.initials: PJ
authors.lastname: Kluger
authors.forename: Ralf
authors.initials: R
authors.lastname: Wyrwa
authors.forename: Jürgen
authors.initials: J
authors.lastname: Weisser
authors.forename: Julia
authors.initials: J
authors.lastname: Maierle
authors.forename: Miriam
authors.initials: M
authors.lastname: Votteler
authors.forename: Claudia
authors.initials: C
authors.lastname: Rode
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.forename: Heike
authors.initials: H
authors.lastname: Walles
authors.forename: Katja
authors.initials: K
authors.lastname: Schenke-Layland
mesh_terms: D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D011091:Polyesters; C033616:poly(lactide)