title: Strain-promoted double-click reaction for chemical modification of azido-biomolecules.
abstract: The strain-promoted "double-click" (SPDC) reaction using Sondheimer diyne, a novel convergent method conjugating three molecules spontaneously, has enabled us to readily modify an azido-biomolecule with a small reporter azido-molecule.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. ikii@bio.titech.ac.jp
authors.forename: Isao
authors.initials: I
authors.lastname: Kii
authors.forename: Akira
authors.initials: A
authors.lastname: Shiraishi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hiramatsu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsushita
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Uekusa
authors.forename: Suguru
authors.initials: S
authors.lastname: Yoshida
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Kudo
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hagiwara
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Hosoya
mesh_terms: D001386:Azides / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D002460:Cell Line; D057930:Click Chemistry / Q000379:methods*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009711:Nucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D001386:Azides; D002241:Carbohydrates; D005456:Fluorescent Dyes; D008055:Lipids; D009711:Nucleotides; D011506:Proteins