Structural basis for the decrease in the outward potassium channel current induced by lanthanum.
The current of the outward K+ channel in the cell of horseradish treated with La3+ and the direct interaction between La3+ and the K+ channel protein were investigated using the whole-cell patch-clamp technique, molecular dynamics simulation, and quantum chemistry calculation methods. It was found for the first time that La3+ decreases the current of the K+ channel in the horseradish mesophyll cell. The decrease results from the formation of a coordination bond and hydrogen bond between La3+ and the K+ channel protein in the plasma membrane. The direct interaction destroys the native structure of the K+ channel protein, disturbing the function of the K+ channel protein in the cells. The results can provide the theoretical foundation for understanding the interaction between metal ions (especially high-valence metal ions) and the channel protein in organisms, including animal and plant cells.
Wang
Li Hong
LH
Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210046, People's Republic of China.
Jiang
Na
N
Zhao
Bo
B
Li
Xiao Dong
XD
Lu
Tian Hong
TH
Ding
Xiao Lan
XL
Huang
Xiao Hua
XH
D000818:Animals; D031215:Armoracia / Q000166:cytology; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007811:Lanthanum / Q000378:metabolism*; D058503:Mesophyll Cells / Q000166:cytology / Q000378:metabolism; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D011789:Quantum Theory
D010940:Plant Proteins; D015221:Potassium Channels; D007811:Lanthanum; D011188:Potassium