Ultra-fast adenosine 5'-triphosphate, adenosine 5'-diphosphate and adenosine 5'-monophosphate detection by pressure-assisted capillary electrophoresis UV detection.
Herein, we report a new CE method to measure adenine nucleotides adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in red blood cells. For this purpose, 20 mmol/L sodium acetate buffer at pH 3.80 was used as running electrolyte, and the separation was performed by the simultaneous application of a CE voltage of 25 kV and an overimposed pressure of 0.2 psi from inlet to outlet. A rapid separation of these analytes in less than 1.5 min was obtained with a good reproducibility for intra- and inter-assay (CV<4 and 8%, respectively) and an excellent analytical recovery (from 98.3 to 99%). The applicability of our method was proved by measuring adenine nucleotides in red blood cells.
Zinellu
Angelo
A
Department of Biomedical Sciences and Centre of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy. angelozinellu@libero.it
Sotgia
Salvatore
S
Scanu
Bastianina
B
Pisanu
Elisabetta
E
Sanna
Manuela
M
Franca Usai
Maria
M
Deiana
Luca
L
Carru
Ciriaco
C
D000244:Adenosine Diphosphate / Q000097:blood / Q000302:isolation & purification*; D000249:Adenosine Monophosphate / Q000097:blood / Q000302:isolation & purification*; D000255:Adenosine Triphosphate / Q000097:blood / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D011312:Pressure; D015203:Reproducibility of Results; D013696:Temperature
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate