Calf production from vitrified bovine sexed embryos following in-straw dilution.
The objective of this study was to develop an in-straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified-warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in-straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in-straw dilution (Non-evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non-evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in-straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in-straw method and that the vitrified and warmed sexed embryos can develop to term.
Akiyama
Kiyoshi
K
Kanagawa Prefectural Livestock Industry Technical Center, Ebina, Kanagawa. akiyama.xqnm@pref.kanagawa.jp
Kobayashi
Jun
J
Sato
Yoshimasa
Y
Sata
Ryuichi
R
Ohashi
Megumi
M
Sasaki
Emi
E
Oda
Yorimasa
Y
Ogawa
Yoshio
Y
Ueda
Shuji
S
Nabenishi
Hisashi
H
Matoba
Satoko
S
D000818:Animals; D002417:Cattle / Q000502:physiology*; D004624:Embryo Transfer / Q000379:methods*; D012732:Sex Determination Analysis; D058989:Vitrification