Rubritalea halochordaticola sp. nov., a carotenoid-producing verrucomicrobial species isolated from a marine chordate.
A gram-negative-staining, obligately aerobic, non-motile, rod-shaped and chemoheterotrophic bacterium, designated strain MN1-1006(T), was isolated from an ascidian (sea squirt) sample, and was studied using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the new isolate shared approximately 93-99% sequence similarity with recognized species of the genus Rubritalea within the phylum 'Verrucomicrobia'. DNA-DNA hybridization values between strain MN1-1006(T) and Rubritalea squalenifaciens HOact23(T) and Rubritalea sabuli YM29-052(T) were 57% and 14.5%, respectively. Strain MN1-1006(T) produced carotenoid compounds that rendered the cell biomass a reddish pink colour. The strain also contained squalene. The cell-wall peptidoglycan of the novel strain contained muramic acid and meso-diaminopimelic acid. The DNA G+C content of strain MN1-1006(T) was 51.4 mol%. The major cellular fatty acids were iso-C(14:0), iso-C(16:0) and anteiso-C(15:0). The major isoprenoid quinone was MK-9. On the basis of these data, it was concluded that strain MN1-1006(T) represents a novel species of the genus Rubritalea, for which the name Rubritalea halochordaticola sp. nov. is proposed. The type strain is MN1-1006(T )( = KCTC 23186(T) = NBRC 107102(T)).
Yoon
Jaewoo
J
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Matsuda
Satoru
S
Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Adachi
Kyoko
K
Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Kasai
Hiroaki
H
Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002338:Carotenoids / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014561:Urochordata / Q000382:microbiology*; D061327:Verrucomicrobia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D009112:Muramic Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D002338:Carotenoids; C073355:menaquinone 9; D003960:Diaminopimelic Acid