Advantages and considerations in the confirmation of mitochondrial DNA mutations by denaturing HPLC and pyrosequencing.
Human mitochondrial DNA (mtDNA) encodes 13 polypeptides essential for oxidative phosphorylation. Because of the unique features of "replicative segregation" and "threshold expression" of mtDNA genetics, identification of homoplasmy versus heteroplasmy status is critical. Results from various detection methods may lead to different interpretations on formation or outcome of mtDNA mutations, such as the conclusion of somatic mutation versus genetic drift in cancers. Denaturing high-performance liquid chromatography (DHPLC) and pyrosequencing (PSQ) have recently been employed to confirm the presence of heteroplasmy of mtDNA because of their high sensitivity in detecting heteroplasmic mutations compared with direct sequencing. Moreover, PSQ has superior ability in quantifying percentage of heteroplasmy. However, there could be disagreement between these two techniques and several issues specific for mtDNA should be taken into consideration. Particularly, DHPLC analysis should be more prone to be interfered by nuclear mitochondrial sequences (Numts), if it is coamplified with mtDNA, than PSQ analysis.
Yen
Hsiu-Chuan
HC
Department of Medical Biotechnology and Laboratory Science/Graduate Institute of Medical Biotechnology, Taoyuan, Taiwan. yen@mail.cgu.edu.tw
Hsu
Wei-Chien
WC
Lin
Chih-Lung
CL
Chen
Guang-Wu
GW
Huang
Yu-Hsiang
YH
D002467:Cell Nucleus / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005821:Genetic Techniques; D006801:Humans; D009154:Mutation; D010085:Oxidative Phosphorylation; D017422:Sequence Analysis, DNA / Q000379:methods*
D004272:DNA, Mitochondrial