Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.
Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.
Hartmann
Thomas
T
Research Center for Infectious Diseases, Julius-Maximilians-University Würzburg, D-97080 Würzburg, Germany.
Dümig
Michaela
M
Jaber
Basem M
BM
Szewczyk
Edyta
E
Olbermann
Patrick
P
Morschhäuser
Joachim
J
Krappmann
Sven
S
D001232:Aspergillus fumigatus / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics
D005819:Genetic Markers; D009841:Oligonucleotides; D004254:DNA Nucleotidyltransferases; C077570:Site-specific recombinase