The effects of melatonin on burn-induced inflammatory responses and coagulation disorders in rats.
The aim of this study was to investigate the effects of melatonin on burn-induced inflammatory responses and coagulation disorders in a rat model. Under ether anesthesia, shaved dorsa of rats were exposed to 90 degrees C for 10 s to induce burn injury. Melatonin (10 mg/kg) was administered immediately and after 12 h. Standard coagulation tests (prothrombin activity [PA] and activated partial thromboplastin time [aPTT]), platelet number and morphology; proinflammatory markers (C-reactive protein [CRP] and fibrinogen) and lipid peroxidation marker (malondialdehyde [MDA]) levels were assayed. Thermal injury increased the levels of MDA (by 76%, P < 0.0001), CRP (by 33%, P < 0.0001), fibrinogen (4.5-fold, P < 0.0001) and PA (by 37%; P < 0.01). Changes in aPTT and platelet numbers were nonsignificant. Melatonin diminished the elevated CRP and fibrinogen levels, normalized MDA levels, platelet morphology and decreased PA. A positive association of MDA with fibrinogen and MDA with PA were noted after melatonin treatment. To conclude, melatonin as an antioxidant and anti-inflammatory agent exerted a suppressive effect on burn-induced disorders in blood coagulation and might be useful in the prevention of disseminated intravascular microthrombosis.
Bekyarova
G
G
Department of Pathophysiology, Medical University of Varna, Varna, Bulgaria. gabekyarova2001@yahoo.com
Tancheva
S
S
Hristova
M
M
D000818:Animals; D001778:Blood Coagulation Disorders / Q000188:drug therapy*; D002056:Burns / Q000097:blood / Q000188:drug therapy*; D002097:C-Reactive Protein / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008550:Melatonin / Q000494:pharmacology / Q000627:therapeutic use*; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats
D008315:Malondialdehyde; D005340:Fibrinogen; D002097:C-Reactive Protein; D008550:Melatonin