title: Induction of mRNA expression of osteogenesis-related genes by guaiacol in human dental pulp cells.
abstract: To investigate the stimulating effect of endodontic medications on the mRNA expression of some osteogenesis-related genes associated with reparative dentinogenesis and hard-tissue formation, human dental pulp cells (D824 cells) were treated with calcium hydroxide (Ca (OH)(2)), formocresol, or guaiacol. The effect on growth was determined by growth curves of D824 cells treated for 1-3 days with 0.03-0.3 mM Ca (OH)(2), 0.0007%-0.0014% formocresol, or 0.24-2.43 mM guaiacol. The mitotic activity of individual cells and the mRNA expression of the osteogenesis-related genes for alkaline phosphatase (ALP), type I collagen (COL-1), and bone sialoprotein (BSP) in the cells treated for 24 h with the same concentrations of the medications as described above were determined by colony-forming efficiency and by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis, respectively. Cellular growth and mitotic activity were scarcely affected by Ca (OH)(2), but were significantly reduced by formocresol or guaiacol. The mRNA expression of the osteogenesis-related genes was little affected by Ca (OH)(2) or formocresol, but was significantly enhanced by guaiacol. The results indicate that guaiacol may stimulate the mRNA expression of genes associated with reparative dentinogenesis and hard-tissue formation in human dental pulp cells, suggesting that the novel property of guaiacol provides new insights into the utilization of guaiacol in endodontic therapy.
authors.affiliation: Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kato
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shirayama
authors.forename: Takeo W
authors.initials: TW
authors.lastname: Tsutsui
authors.forename: Takeki
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005566:Formocresols / Q000494:pharmacology; D006139:Guaiacol / Q000494:pharmacology*; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D008938:Mitosis / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000187:drug effects; D013268:Stimulation, Chemical; D013997:Time Factors; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D005566:Formocresols; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D012388:Root Canal Irrigants; D012795:Sialoglycoproteins; C031025:formocresol; D006139:Guaiacol; D000469:Alkaline Phosphatase; D002126:Calcium Hydroxide