Effect of bFGF on invasion of ovarian cancer cells through the regulation of Ets-1 and urokinase-type plasminogen activator.
The aim of the study was to explore the role of basic fibroblast growth factor (bFGF) in ovarian cancer progression. This was done by investigating the effects of bFGF on both the secretion of urokinase-type plasminogen activator (uPA) and the invasion of tumor cells in SKOV(3) ovarian cancer cells. Human ovarian cancer cell line SKOV(3) was cultured in vitro. The expression of uPA gene and protein was induced in SKOV(3) cells; the impact of bFGF on the expression of uPA gene in SKOV(3) cells was studied by RT-PCR, and the impact of bFGF on the expression of uPA protein was tested by ELISA. Ets-1 antisense oligonucleotides were transfected into SKOV(3) cells by liposome protocol. The effects of bFGF on Ets-1 expression and the invasion ability of SKOV(3) cells were determined both before and after exposure to different concentrations of bFGF for 24 h. The expression of both uPA gene and protein was induced in SKOV(3) cells, p < 0.05. The expression of uPA was suppressed by Ets-1 antisense oligonucleotides in SKOV(3) cells, p < 0.05. The invasion ability of SKOV(3) cells was increased by 2.3-fold, and this effect was also suppressed by Ets-1 antisense oligonucleotides. bFGF can enhance the invasion ability of ovarian cancer cells in vitro by inducing the expression of uPA, and this effect is also regulated by the transcription factor Ets-1.
Li
Ting
T
Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital Affiliated to Tongji University, Shanghai, China. lixiaote@hotmail.com
Jiang
Sen
S
D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense; D009994:Osmolar Concentration; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D050784:Proto-Oncogene Protein c-ets-1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism*
C494041:ETS1 protein, human; C100666:KCB-1 protein, recombinant; D016376:Oligonucleotides, Antisense; D050784:Proto-Oncogene Protein c-ets-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D014568:Urokinase-Type Plasminogen Activator