Transcriptional profiling of host gene expression in chicken embryo lung cells infected with laryngotracheitis virus.
BACKGROUND
Infection by infectious laryngotracheitis virus (ILTV; gallid herpesvirus 1) causes acute respiratory diseases in chickens often with high mortality. To better understand host-ILTV interactions at the host transcriptional level, a microarray analysis was performed using 4 x 44 K Agilent chicken custom oligo microarrays.


RESULTS
Microarrays were hybridized using the two color hybridization method with total RNA extracted from ILTV infected chicken embryo lung cells at 0, 1, 3, 5, and 7 days post infection (dpi). Results showed that 789 genes were differentially expressed in response to ILTV infection that include genes involved in the immune system (cytokines, chemokines, MHC, and NF-kappaB), cell cycle regulation (cyclin B2, CDK1, and CKI3), matrix metalloproteinases (MMPs) and cellular metabolism. Differential expression for 20 out of 789 genes were confirmed by quantitative reverse transcription-PCR (qRT-PCR). A bioinformatics tool (Ingenuity Pathway Analysis) used to analyze biological functions and pathways on the group of 789 differentially expressed genes revealed that 21 possible gene networks with intermolecular connections among 275 functionally identified genes. These 275 genes were classified into a number of functional groups that included cancer, genetic disorder, cellular growth and proliferation, and cell death.


CONCLUSIONS
The results of this study provide comprehensive knowledge on global gene expression, and biological functionalities of differentially expressed genes in chicken embryo lung cells in response to ILTV infections.
Lee
Jeong Yoon
JY
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Song
Joon Jin
JJ
Wooming
Ann
A
Li
Xianyao
X
Zhou
Huaijun
H
Bottje
Walter G
WG
Kong
Byung-Whi
BW
D000818:Animals; D002642:Chick Embryo; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D001352:Herpesvirus 1, Gallid / Q000502:physiology*; D008168:Lung / Q000378:metabolism*