Evaluation of suitable solvents for testing the anti-proliferative activity of triclosan - a hydrophobic drug in cell culture.
Triclosan, a broad spectrum antibiotic is currently being evaluated for its anti-cancer property. Though several solvents are available to dissolve lipophilic (hydrophobic) drugs, solubility and toxicity aspects pose a challenge, when combined with the cell culture medium. In this paper, we present a simple approach based on physico-chemical and biologic criteria to choose a suitable solubilizing agent to study the anti-proliferative property of triclosan in breast cancer cell line MCF-7. Triclosan was dissolved in five different solvents viz. DMSO, absolute ethanol, 1 N NaOH, 55% polyethylene glycol + 45% ethanol mixture (PEM) and acetone and diluted with the culture medium (1 mg/ml). Although triclosan dissolved completely in all five solvents, on dilution with culture medium, turbidity was observed in DMSO, 1 N NaOH and ethanol. Cell viability was 95.23% in 10 microl of acetone, when compared with 49.45% at the same volume of PEM. This non-toxic nature of acetone was supported by DNA fragmentation analysis and phase contrast microscopy. A significant decrease in cancer cell proliferation at 100 microg/ml of acetone-solubilized triclosan, compared with 100 microg/ml of PEM-solubilized triclosan (p<0.05) indicated stronger anti-proliferative effect and greater drug-sensitivity of triclosan when solubilized in acetone. Results showed that acetone-solubilized triclosan was suitable for anti-cancer investigations in cultured MCF-7 cells.
Vandhana
S
S
Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, 18, College Road, Nungambakkam, Chennai 600 006, India.
Deepa
P R
PR
Aparna
G
G
Jayanthi
U
U
Krishnakumar
S
S
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D012995:Solubility; D012997:Solvents; D014260:Triclosan / Q000494:pharmacology*
D000970:Antineoplastic Agents; D012997:Solvents; D014260:Triclosan