Chromosomal location-dependent nonstochastic onset of odor receptor expression.
As odorant receptors (ORs) are thought to be critical determinants of olfactory sensory neuron (OSN) axon targeting and organization, we examined the spatiotemporal onset of mice ORs expression from the differentiation of OSNs in the olfactory placode to an aging olfactory epithelium. ORs were first detected in the placode at embryonic day 9 (E9), at the onset of OSN differentiation but before axon extension. By E13, 22 of 23 ORs were expressed. Onset of individual OR expression was diverse; levels and patterns of expression were unique for each OR. Regional distribution of ORs within zones of the olfactory epithelium appeared stable across development; adult-like patterns were observed by E13. Finally, analysis of OR expression and chromosomal location suggests that ORs are not stochastically expressed; they show evidence of coordinated expression. Collectively, these studies demonstrate that ORs are not equally represented in the "olfactome" across an animal's lifespan.
Rodriguez-Gil
Diego J
DJ
Departments of Neurosurgery and Neurobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Treloar
Helen B
HB
Zhang
Xiaohong
X
Miller
Alexandra M
AM
Two
Aimee
A
Iwema
Carrie
C
Firestein
Stuart J
SJ
Greer
Charles A
CA
D000465:Algorithms; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology; D002874:Chromosome Mapping / Q000379:methods; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis / Q000379:methods; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D019006:Neural Cell Adhesion Molecules; D018035:Receptors, Odorant; D014404:Tubulin