TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through beta1 integrin-mediated signaling.
Odontoblasts are involved in tooth repair and regeneration as well as dentin formation. The aim of this study was to examine whether delivery of heat shock protein 27 (Hsp27) into cells using a TAT fusion protein system (TAT-Hsp27) enhances adhesion and migration of murine dental papilla-derived MDPC-23 cells. Hsp27 was delivered into cells by the TAT-fusion protein system. To examine whether TAT-Hsp27 affects the viability of MDPC-23 cells, MTT assay was performed. The effect of TAT-Hsp27 on adhesion and migration of MDPC-23 cells was determined using type I collagen-coated plates and a commercial kit, respectively. In addition, a precise molecular mechanism was examined by Western blot analysis and focal adhesion activity. TAT-fusion protein system delivered Hsp27 into cells successfully. Transduction of TAT-Hsp27 induced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Moreover, transduction of TAT-Hsp27 increased the protein expression of beta1 integrin and focal adhesion formation, and induced phosphorylation of FAK and ERK. TAT-Hsp27-induced migration of MDPC-23 cells was restored by treatment of anti-beta1 integrin antibody. These findings suggest that TAT-Hsp27 promotes adhesion and migration of MDPC-23 cells via beta1 integrin-mediated signaling and is a promising candidate for therapeutic application of dental pulp regeneration.
Park
Jong-Hwan
JH
Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Korea.
Yoon
Ji-Hye
JH
Lim
Young-Sin
YS
Hwang
Ho-Keel
HK
Kim
Soo-A
SA
Ahn
Sang-Gun
SG
Yoon
Jung-Hoon
JH
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D003771:Dental Papilla / Q000166:cytology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D019012:Integrin beta1 / Q000378:metabolism*; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
D015696:Gene Products, tat; D055551:HSP27 Heat-Shock Proteins; D019012:Integrin beta1; D011993:Recombinant Fusion Proteins; D051417:Focal Adhesion Kinase 1; D048049:Extracellular Signal-Regulated MAP Kinases