title: Intracellular imaging of HeLa cells by non-functionalized NaYF4 : Er3+, Yb3+ upconverting nanoparticles.
abstract: We report on the efficient incorporation of non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles inside HeLa live cancer cells by direct endocytosis. The efficient two-photon excited near-infrared-to-visible upconversion fluorescence of these nanoparticles is then used to obtain high-contrast intracellular fluorescence images of single cells. These images reveal a redistribution of the nanoparticles inside the cell as the incubation time increases. Thus, non-functionalized NaYF(4) : Er(3+), Yb(3+) nanoparticles emerge as very promising fluorescence probes for real-time imaging of cellular dynamics.
authors.affiliation: Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada.
authors.forename: Fiorenzo
authors.initials: F
authors.lastname: Vetrone
authors.forename: Rafik
authors.initials: R
authors.lastname: Naccache
authors.forename: Angeles
authors.initials: A
authors.lastname: Juarranz de la Fuente
authors.forename: Francisco
authors.initials: F
authors.lastname: Sanz-Rodríguez
authors.forename: Alfonso
authors.initials: A
authors.lastname: Blazquez-Castro
authors.forename: Emma Martin
authors.initials: EM
authors.lastname: Rodriguez
authors.forename: Daniel
authors.initials: D
authors.lastname: Jaque
authors.forename: José García
authors.initials: JG
authors.lastname: Solé
authors.forename: John A
authors.initials: JA
authors.lastname: Capobianco
mesh_terms: D004871:Erbium / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D015018:Ytterbium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
chemical_list: D015019:Yttrium; D004871:Erbium; D015018:Ytterbium