Distinct early inflammatory events during ear tissue regeneration in mice selected for high inflammation bearing Slc11a1 R and S alleles.
High inflammatory AIRmax mice homozygous for Slc11a1 R and S alleles were produced. AIRmax(SS) mice showed faster ear tissue regeneration than AIRmax(RR) mice, suggesting that the S allele favored tissue restoration. Here, we investigated the gene expression profiles and the inflammatory reactions of AIRmax(RR) and AIRmax(SS) mice during the initial phase of ear tissue regeneration. We observed superior levels of analysis of wound myeloperoxidase and edema in AIRmax(SS) mice, although similar cell influx was verified in both lines. Of the genes, 794 were up- and 674 down-regulated in AIRmax(RR), while 735 genes were found to be up- and 1616 down-regulated in AIRmax(SS) mice 48 h after punch. Both mouse lines showed significant over-represented genes related to cell proliferation; however AIRmax(SS) displayed up-regulation of inflammatory response genes. Quantitative PCR experiments showed higher expressions of Tgfb1, Dap12 and Trem1 genes in AIRmax(SS) mice. These results indicate that Slc11a1 gene modulated the early inflammatory events of ear tissue regeneration.
Canhamero
Tatiane
T
Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, Brasil.
Reines
Brandon
B
Peters
Luciana C
LC
Borrego
Andrea
A
Carneiro
Patricia S
PS
Albuquerque
Layra L
LL
Cabrera
Wafa H
WH
Ribeiro
Orlando G
OG
Jensen
Jose R
JR
Starobinas
Nancy
N
Ibañez
Olga M
OM
De Franco
Marcelo
M
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics* / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004431:Ear, External / Q000293:injuries / Q000502:physiology; D005260:Female; D015870:Gene Expression; D007249:Inflammation / Q000235:genetics* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009195:Peroxidase / Q000378:metabolism; D040641:Quantitative Trait Loci; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000235:genetics* / Q000502:physiology*; D014945:Wound Healing / Q000235:genetics / Q000502:physiology; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D017931:DNA Primers; D009195:Peroxidase; D000099173:Natural Resistance-associated Macrophage Protein 1