Effects of different wavelengths of light on lignin peroxidase production by the white-rot fungi Phanerochaete chrysosporium grown in submerged cultures.
In this study, the effects of different wavelengths of light (UV, blue, green, yellow, red) and white light on lignin peroxidase (LiP), protein, biomass and exo-polysaccharide production and glucose uptake by Phanerochaete chrysosporium BKM-F-1767 were determined. The experiments were conducted under aerated (CS) and oxygenated (RS) culture conditions. The results showed that only green light significantly increased maximum LiP production (by 20% and 27% in CS and RS cultures respectively). Green light also increased biomass production in oxygenated cultures (RS). Blue and UV light both significantly reduced maximum LiP activity. Yellow, red and white lights had mixed effects on culture properties. This is the first time that the effects of different wavelengths of light on lignin peroxidase production and other culture properties have been investigated. The novel findings may be important in improving the yield of lignin modifying enzymes for biomass conversion processes and understanding their regulation.
Ramírez
David A
DA
Department of Food, Agricultural and Biological Engineering, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA.
Muñoz
Sandra V
SV
Atehortua
Lucia
L
Michel
Frederick C
FC
D000704:Analysis of Variance; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008027:Light; D010544:Peroxidases / Q000096:biosynthesis*; D020075:Phanerochaete / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000528:radiation effects*; D010935:Plant Diseases / Q000382:microbiology*; D011134:Polysaccharides / Q000096:biosynthesis
D003470:Culture Media; D005656:Fungal Proteins; D011134:Polysaccharides; D010544:Peroxidases; C042858:lignin peroxidase; D005947:Glucose