title: Compounds from Viburnum sargentii Koehne and evaluation of their cytotoxic effects on human cancer cell lines.
abstract: Compounds were isolated from a methanol extract of the dried stem barks of Viburnum sargentii Koehne. The structures of the compounds, namely 9'-O-methylvibsanol (3), furcatoside A (4) and lareciresinol (5) were elucidated by analysis of spectroscopic data and comparison with values for previously known analogues. In addition, (+)-catechin (1), (+)-epicatechin (2) were also isolated. This work also examined the cytotoxic effects of three compounds 3-5 (25-100 microM) in MCF-7 and A549 cells after 24, 48 and 72 h of exposure. Our results showed that 9'-O-methylvibsanol (3) exhibited strong concentration-dependent anticancer effects according to the MTT assay and produced morphological changes consistent with apoptosis, as confirmed by Ho3342 staining analysis revealed that more apoptotic cells were observed after 9'-Omethylvibsanol (3) treatment.
authors.affiliation: Department of Forest Products and Biotechnology, College of Forest Sciences, Kookmin University, Seoul, 136-702, Korea. kieun@kookmin.ac.kr
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Bae
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Chong
authors.forename: Dong-Sup
authors.initials: DS
authors.lastname: Kim
authors.forename: Young-Woong
authors.initials: YW
authors.lastname: Choi
authors.forename: Young-Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Young-Kyoon
authors.initials: YK
authors.lastname: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D013057:Spectrum Analysis; D013997:Time Factors; D027925:Viburnum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts