title: The cytoskeletal regulator zyxin is required for viability in Drosophila melanogaster.
abstract: The zyxin family of proteins function as cytoskeletal regulators in adhesion, actin assembly, and cell motility. Though fibroblasts derived from zyxin-null mice show striking defects in motility and response to mechanical stimuli, the mice are viable and fertile. In Drosophila melanogaster, the family is represented by a single homologue, Zyx102. To study the role of zyxin during development, we generated a zyx102 RNA-interference transgenic line that allows for the conditional knockdown of Zyx102. When UAST-zyx102-dsRNAi expression is driven broadly by Actin5C-GAL4, loss of Zyx102 results in lethality during the pharate adult stage, a narrow developmental window during which the fly must molt, resorb molting fluid, fill adult trachea with air, and execute a behavioral program to eclose. Zyx102 knockdown animals attempt to emerge, but their adult trachea do not fill with air. If dissected from the pupal case, knockdown individuals appear morphologically normal, but remain inviable.
authors.affiliation: Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112-5550, USA.
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Renfranz
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Blankman
authors.forename: Mary C
authors.initials: MC
authors.lastname: Beckerle
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D011679:Pupa / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D014132:Trachea / Q000166:cytology / Q000196:embryology; D060589:Zyxin
chemical_list: D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C499520:Zyx protein, Drosophila; D060589:Zyxin