[Source identification of gastric cancer tissue with discriminatory analysis].
OBJECTIVE
To evaluate discriminatory analysis on source identification of gastric cancer tissue based on the number of matched STR locus or identical allele.


METHODS
Twenty two pairs of fresh gastric cancer tissue and homologous normal tissue were genotyped with Identifiler kit. Frequencies of STR genotypic alteration (STR(GA)), the number of matched STR locus without identical allele (A0), with 1 identical allele (A1), or with 2 identical alleles (A2) and the number of total identical alleles (IAn) were calculated with counting method. A1, A2 and IAn were evaluated with Fisher discriminant functions to determine the source of each gastric cancer tissue. Effectiveness of the identification of gastric cancer tissue was evaluated with error rate.


RESULTS
The total frequency STR(GA) was 3.03% (95% CI: 1.46%-4.88%). There were 31.38% (95% CI: 13.86%-54.87%) of gastric cancer samples carried at least one STR locus with STRGA. It was confirmed by the Fisher discriminant functions that each of the 22 gastric cancer tissue samples came from its homologous normal tissue with an error rate of 0.00%.


CONCLUSIONS
Frequency of STRGA in gastric cancer tissue was high. Fisher discriminant functions based on the number of identical alleles or matched STR loci could be a feasible method for source identification of body for gastric cancer tissue samples.
Li
Cheng-tao
CT
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. lichengtaohla@163.com
Zhao
Shu-min
SM
Zhang
Su-hua
SH
Li
Li
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D000483:Alleles; D016002:Discriminant Analysis; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics*; D020080:Tandem Repeat Sequences / Q000235:genetics*; D055815:Young Adult