title: Immobilization of genetically engineered fusion proteins on gold-decorated carbon nanotube hybrid films for the fabrication of biosensor platforms.
abstract: We have demonstrated the fabrication of the biosensor platforms by means of the integration of the genetically engineered fusion proteins and the uniform gold nanoparticle-deposited multi-walled nanotube hybrid (Au-MWNT-HB) films for the detection of C-reactive protein (CRP). Au-MWNT-HB films were used as a good electrochemical transducer due to their excellent electrical properties and large surface areas for the signal transduction, while the genetically engineered fusion proteins, or 6His-GBP-SpA fusion proteins, specifically bind onto the surface of the Au-MWNT-HB films and efficiently immobilize bioreceptors for the detection of CRP. As-obtained biosensor platforms were characterized by electrochemical and optical analysis and revealed better performance compared to conventional Au-based biosensors. The concept delineated herein opens a new insight into nanobiotechnology through the integration of genetically engineered biomaterials with carbon nanotube (CNT)-based nanohybrids for emerging applications.
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: HoSeok
authors.initials: H
authors.lastname: Park
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: Huh
authors.forename: Bong Gill
authors.initials: BG
authors.lastname: Choi
authors.forename: Sungho
authors.initials: S
authors.lastname: Ko
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Won Hi
authors.initials: WH
authors.lastname: Hong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015202:Protein Engineering
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006046:Gold