[A verification study on the genes associated with laryngeal squamous cell carcinoma by cDNA microarray].
OBJECTIVE
Our previous study revealed many genes differentially expressed in human laryngeal squamous cell carcinoma tissues (LSCC) and related adjacent normal tissues. We verificated the differentiated expressions of target genes, possibly related to LSCC. And these results can be foundations of further study of these target genes function.


METHODS
SENP1, CD109, Laminin alpha 2, Laminin alpha 3 were selected according to the cDNA microarray results. The expression of these genes mRNA and protein were detected by RT-PCR and Western blot in 12 cases of LSCC and related adjacent normal tissues.


RESULTS
The mRNA expression of SENP1, CD109 and Laminin alpha 3 were significantly higher while Laminin alpha 2 were significantly lower in LSCC tissues than in corresponding adjacent normal tissues by Semiquantitative RT-PCR. Western blot analysis revealed SENP1, CD109 protein expression were significantly higher in LSCC tissues than in corresponding adjacent normal tissues.


CONCLUSIONS
SENP1, CD109, Laminin alpha 2 and Laminin alpha 3 may correlated with tumorigenesis and development of LSCC and can provide beneficial clue for study pathogenesis of LSCC in molecular level.
Shen
Xiaohui
X
Department of Otorhinolaryngology-Head and Neck Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, 210008, China.
Ni
Rongsheng
R
Qian
Xiaoyun
X
Yu
Chenjie
C
Wu
Haiyan
H
Ni
Jie
J
Zhu
Wenyan
W
Gao
Xia
X
D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000235:genetics; D058851:GPI-Linked Proteins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis
D015703:Antigens, CD; C120523:CD109 protein, human; D058851:GPI-Linked Proteins; D007797:Laminin; D009363:Neoplasm Proteins; C102645:laminin alpha 2; C095567:laminin alpha 3; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases