Redox electrodeposition polymers: adaptation of the redox potential of polymer-bound Os complexes for bioanalytical applications.
The design of polymers carrying suitable ligands for coordinating Os complexes in ligand exchange reactions against labile chloro ligands is a strategy for the synthesis of redox polymers with bound Os centers which exhibit a wide variation in their redox potential. This strategy is applied to polymers with an additional variation of the properties of the polymer backbone with respect to pH-dependent solubility, monomer composition, hydrophilicity etc. A library of Os-complex-modified electrodeposition polymers was synthesized and initially tested with respect to their electron-transfer ability in combination with enzymes such as glucose oxidase, cellobiose dehydrogenase, and PQQ-dependent glucose dehydrogenase entrapped during the pH-induced deposition process. The different polymer-bound Os complexes in a library containing 50 different redox polymers allowed the statistical evaluation of the impact of an individual ligand to the overall redox potential of an Os complex. Using a simple linear regression algorithm prediction of the redox potential of Os complexes becomes feasible. Thus, a redox polymer can now be designed to optimally interact in electron-transfer reactions with a selected enzyme.
Guschin
Dmitrii A
DA
Analytische Chemie-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany.
Castillo
John
J
Dimcheva
Nina
N
Schuhmann
Wolfgang
W
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004595:Electroplating; D004800:Enzymes, Immobilized / Q000737:chemistry; D017892:Osmium Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
D004800:Enzymes, Immobilized; D017892:Osmium Compounds; D011108:Polymers