Effects of UV-B radiation in morpho-genetic characters of Gnaphalium luteo-album.
Genetic analyses of Gnaphalium's DNA showed a high degree of polymorphism in UV-B irradiated plants when compared to controls. Among the five tested primers the four ISSR primers selected for this analysis generated a total of 189 fragments. A high proportion of polymorphic bands ranging from 70% to 28% were found using these ISSR markers. Nei and Li similarity indexes [1] were used to evaluate genetic divergence among plants. The dendrograms obtained using these markers efficiently separate plants from different treatments. A linear relationship was observed between UV-B dose and percentage of dissimilarity which may be related to DNA damage caused by the different UV-B treatments.
Cuadra
Pedro
P
Universidad de Magallanes, Facultad de Ciencias, P.O. Box 113-D, Punta Arenas, Chile. pedro.cuadra@umag.cl
Vargas
Daniela
D
Fajardo
Víctor
V
Herrera
Raúl
R
D004249:DNA Damage; D018744:DNA, Plant / Q000032:analysis; D036622:Gnaphalium / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D014466:Ultraviolet Rays
D018744:DNA, Plant; D010940:Plant Proteins