[Effects of cocaine on activities of ATPase, LDH and SDH in mouse splenocytes].
OBJECTIVE
To examine the effects of cocaine on the activities of ATPase, LDH and SDH in cultured mouse splenocytes in vitro.


METHODS
The ATPase, LDH and SDH activities in mouse splenocytes were detected at day 7 after continuous culturing the mouse cells exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL in vitro.


RESULTS
The activities of ATPase, LDH and SDH in mouse splenocytes exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL were significantly decreased after continuous culturing for 7 days.


CONCLUSIONS
The present study demonstrated that cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes in vitro.
Sun
Wen-ping
WP
Department of Forensic Pathology, Taishan Medical College, Taian 271000, China. wenpingsun@163.com
Lu
Yan-xu
YX
Zhang
Xiao-yu
XY
Tang
Wei-wei
WW
Huang
Qing-yu
QY
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003042:Cocaine / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases; D003042:Cocaine