Differential profiles of gene expression in grouper Epinephelus coioides, infected with Singapore grouper iridovirus, revealed by suppression subtractive hybridization and DNA microarray.
Suppression subtractive hybridization (SSH) was used to generate a subtracted cDNA library enriched with gene transcripts differentially expressed in the spleen of orange-spotted grouper Epinephelus coioides after 5 days of infection with Singapore grouper iridovirus (SGIV). In the forward and reverse-subtracted libraries, 260 and 153 non-redundant expressed sequence tags (EST), respectively, were identified. These annotated genes responding to SGIV infection were grouped into eight gene categories related to immunity, cell structure, transcription-translation, cell signalling, metabolism, mitochondrial proteins, ribosomal proteins and unknown or hypothetical proteins. A DNA microarray containing all the differentially expressed genes was constructed, and the gene expression patterns in different tissues were investigated in virus-infected E. coioides. Of these genes, four associated with the infection processes were identified and further investigated by quantitative real-time PCR. These results provide new insights into the molecular basis of host-pathogen interactions in E. coioides, and will help the development of control strategies against SGIV infection.
Xu
D
D
State Key Laboratory of Biocontrol, School of life sciences, Sun Yat-sen University, 135 West Xingang Road, Guangzhou 510275, China.
Wei
J
J
Cui
H
H
Gong
J
J
Yan
Y
Y
Lai
R
R
Qin
Q
Q
D000818:Animals; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000235:genetics* / Q000821:virology; D020869:Gene Expression Profiling; D015723:Gene Library; D054884:Host-Pathogen Interactions; D017994:Iridovirus / Q000472:pathogenicity*; D020411:Oligonucleotide Array Sequence Analysis; D010473:Perciformes / Q000235:genetics* / Q000821:virology