Tetrahydroxystilbene glucoside attenuates MPP+-induced apoptosis in PC12 cells by inhibiting ROS generation and modulating JNK activation.
It is known that oxidative stress plays a major role in the progression of Parkinson's disease (PD). Previous studies have suggested that 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (TSG), an active component extracted from a traditional Chinese herb Polygonum multiflorum Thunb., has significant antioxidant and free radical-scavenging activities. This is the first study that investigated the protective effects of TSG against MPP(+)-induced apoptosis in PC12 cells and determined the underlying mechanism. The results showed that incubation of PC12 cells with TSG before exposing them to MPP(+) could significantly decrease cell viability loss and reverse cell apoptosis in a dose-dependent manner. The anti-apoptotic effects of TSG were probably mediated via the inhibition of ROS generation and modulation of JNK activation because TSG blocked ROS increase and JNK phosphorylation induced by MPP(+). Taken together, these results indicated that TSG may provide a useful therapeutic strategy for the treatment of neurodegenerative diseases such as PD.
Li
Xiaobing
X
Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, No 17 Changlexi Road, Xi'an 710032, People's Republic of China.
Li
Yan
Y
Chen
Jianzong
J
Sun
Jing
J
Li
Xiaofeng
X
Sun
Xin
X
Kang
Xiaogang
X
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005960:Glucosides / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D005960:Glucosides; D017382:Reactive Oxygen Species; D013267:Stilbenes; D015655:1-Methyl-4-phenylpyridinium