MCP-1 and MIP-1α expression in a model resembling early immune response to dengue.
Dengue virus has become endemic in most tropical urban areas throughout the world, and DHF has appeared concomitantly with this expansion. The intensity of dengue virus replication during the early stages of infection could determine clinical outcomes; therefore, it is important to understand the impact of dengue virus infection on the earliest immune defense against microbial infection, which also strongly regulates the adaptive immune responses. This study was aimed at evaluating the expression of the CC-chemokines MIP-1α/CCL3 and MCP-1/CCL2 in peripheral blood leukocytes using an ex vivo model resembling dengue infection in vivo, in subjects with a well characterized dengue immune background, due to the exceptional Cuban epidemiological situation in dengue. The expression of IFNγ, TNFα and IL10 was also evaluated, giving insight about the role of MCP-1 and MIP-1α in the interplay between innate and adaptive immunity. From individuals with different dengue immune background after dengue virus challenge, increased and different expression of the chemokines and cytokines studied was verified in peripheral blood mononuclear cells, thus demonstrating that the previous immunity to a dengue virus serotype has a strong influence on the early immune response after dengue re-infection.
Sierra
Beatriz
B
Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine Pedro Kouri, Autopista Novia del Mediodia Km. 6 ½, La Lisa, Havana City, Cuba. siebet@ipk.sld.cu
Perez
Ana B
AB
Vogt
Katrin
K
Garcia
Gissell
G
Schmolke
Kathrin
K
Aguirre
Eglys
E
Alvarez
Mayling
M
Volk
Hans-Dieter
HD
Guzman
Maria G
MG
D000328:Adult; D018932:Chemokine CCL2 / Q000378:metabolism*; D054405:Chemokine CCL3 / Q000378:metabolism*; D003715:Dengue / Q000276:immunology* / Q000378:metabolism; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019562:Viral Load
C111835:CCL2 protein, human; C515429:CCL3 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3