title: The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose.
abstract: A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets, haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and 3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles could be affected by prebiotics, which should be taken into account in future studies.
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. hoseinifar@ut.ac.ir
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Hoseinifar
authors.forename: Alireza
authors.initials: A
authors.lastname: Mirvaghefi
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Merrifield
authors.forename: Bagher Mojazi
authors.initials: BM
authors.lastname: Amiri
authors.forename: Saeed
authors.initials: S
authors.lastname: Yelghi
authors.forename: Kazem Darvish
authors.initials: KD
authors.lastname: Bastami
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D004032:Diet / Q000662:veterinary; D005399:Fishes / Q000097:blood* / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D056692:Prebiotics; D044967:Serum / Q000737:chemistry / Q000166:cytology / Q000201:enzymology
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; C120489:oligofructose