Epidermal wound repair is regulated by the planar cell polarity signaling pathway.
The mammalian PCP pathway regulates diverse developmental processes requiring coordinated cellular movement, including neural tube closure and cochlear stereociliary orientation. Here, we show that epidermal wound repair is regulated by PCP signaling. Mice carrying mutant alleles of PCP genes Vangl2, Celsr1, PTK7, and Scrb1, and the transcription factor Grhl3, interact genetically, exhibiting failed wound healing, neural tube defects, and disordered cochlear polarity. Using phylogenetic analysis, ChIP, and gene expression in Grhl3(-)(/-) mice, we identified RhoGEF19, a homolog of a RhoA activator involved in PCP signaling in Xenopus, as a direct target of GRHL3. Knockdown of Grhl3 or RhoGEF19 in keratinocytes induced defects in actin polymerization, cellular polarity, and wound healing, and re-expression of RhoGEF19 rescued these defects in Grhl3-kd cells. These results define a role for Grhl3 in PCP signaling and broadly implicate this pathway in epidermal repair.
Caddy
Jacinta
J
Rotary Bone Marrow Research Laboratories, Parkville, Victoria 3050, Australia.
Wilanowski
Tomasz
T
Darido
Charbel
C
Dworkin
Sebastian
S
Ting
Stephen B
SB
Zhao
Quan
Q
Rank
Gerhard
G
Auden
Alana
A
Srivastava
Seema
S
Papenfuss
Tony A
TA
Murdoch
Jennifer N
JN
Humbert
Patrick O
PO
Parekh
Vishwas
V
Boulos
Nidal
N
Weber
Thomas
T
Zuo
Jian
J
Cunningham
John M
JM
Jane
Stephen M
SM
D000199:Actins / Q000378:metabolism; D000818:Animals; D016764:Cell Polarity / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D004817:Epidermis / Q000196:embryology / Q000293:injuries* / Q000502:physiology*; D005260:Female; D020662:Guanine Nucleotide Exchange Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008954:Models, Biological; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D011247:Pregnancy; D064067:Rho Guanine Nucleotide Exchange Factors; D015398:Signal Transduction; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
D000199:Actins; D004268:DNA-Binding Proteins; C499942:Grhl3 protein, mouse; D020662:Guanine Nucleotide Exchange Factors; C431577:Ltap protein, mouse; D009419:Nerve Tissue Proteins; D064067:Rho Guanine Nucleotide Exchange Factors; D014157:Transcription Factors