title: X-ray diffraction experiment--the last experiment in the structure elucidation process.
abstract: Recent years have brought not only an avalanche of new macromolecular structures, but also significant advances in the protein structure determination methodology only now making its way into structure-based drug discovery. In this chapter, we review recent methodology developments in X-ray diffraction experiments that led to fast and very accurate elucidation of three-dimensional structures of macromolecules. We will discuss the role of data collection as the last experiment performed in the crystal structure determination process. A statistical analysis of diffraction experiments that are reported in the Protein Data Bank (PDB) is also presented.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA.
authors.forename: Maksymilian
authors.initials: M
authors.lastname: Chruszcz
authors.forename: Dominika
authors.initials: D
authors.lastname: Borek
authors.forename: Marcin
authors.initials: M
authors.lastname: Domagalski
authors.forename: Zbyszek
authors.initials: Z
authors.lastname: Otwinowski
authors.forename: Wladek
authors.initials: W
authors.lastname: Minor
mesh_terms: D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D011506:Proteins