Monitoring photobleaching and hemodynamic responses to HPPH-mediated photodynamic therapy of head and neck cancer: a case report.
We present initial results obtained during the course of a Phase I clinical trial of 2-1[hexyloxyethyl]-2-devinylpyropheophorbide-a (HPPH)-mediated photo-dynamic therapy (PDT) in a head and neck cancer patient. We quantified blood flow, oxygenation and HPPH drug photobleaching before and after therapeutic light treatment by utilizing fast, non-invasive diffuse optical methods. Our results showed that HPPH-PDT induced significant drug photobleaching, and reduction in blood flow and oxygenation suggesting significant vascular and cellular reaction. These changes were accompanied by cross-linking of the signal transducer and activator of transcription 3 (STAT3), a molecular measure for the oxidative photoreaction. These preliminary results suggest diffuse optical spectroscopies permit non-invasive monitoring of PDT in clinical settings of head and neck cancer patients.
Sunar
Ulas
U
Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY, 14263, USA. ulas.sunar@roswellpark.org
Rohrbach
Daniel
D
Rigual
Nestor
N
Tracy
Erin
E
Keymel
Ken
K
Cooper
Michele T
MT
Baumann
Heinz
H
Henderson
Barbara H
BH
D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000627:therapeutic use; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005336:Fiber Optic Technology; D006258:Head and Neck Neoplasms / Q000188:drug therapy*; D006439:Hemodynamics / Q000502:physiology*; D006801:Humans; D008297:Male; D038761:Photobleaching / Q000187:drug effects; D010778:Photochemotherapy; D050796:STAT3 Transcription Factor / Q000378:metabolism
D003432:Cross-Linking Reagents; D050796:STAT3 Transcription Factor; D002734:Chlorophyll; C083998:2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a