Presenilin-1 in neuroblastoma cells.
Presenilin-1 expression was investigated in Neuro-2a cells using the alphaPS1Loop antibody. The bulk of the immunoreactivity was found as the approximately 18kDa C-terminal fragment. Hydrogen peroxide treatment of the Neuro-2a cells for 24 hours resulted in a concentration-dependent cytotoxicity with a critical concentration of hydrogen peroxide of approximately 20 mum. Treatment of the cells for 24hr with 10, 20 or 30 mum hydrogen peroxide did not produce obvious effects upon presenilin-1 C-terminal fragment immunoreactivity. It is suggested that the loss of presenilin-1-mediated neuroprotection that has been proposed to occur in Alzheimer's disease is not secondary to hydrogen peroxide-mediated cell stress.
Lindblad
C
C
Department of Pharmacology, UmeåUniversity, S-901 87 Umeå, Sweden.
Hedin
H
H
Andersson
G
G
Fowler
C J
CJ