Adipose-specific deletion of stearoyl-CoA desaturase 1 up-regulates the glucose transporter GLUT1 in adipose tissue.
Stearoyl-CoA desaturase 1 (SCD1) deficiency protects mice from diet-induced obesity and insulin resistance. To understand the tissue-specific role of SCD1 in energy homeostasis, we have generated mice with an adipose-specific knockout of Scd1 (AKO), and report here that SCD1 deficiency increases GLUT1 expression in adipose tissue of AKO mice, but not global SCD1 knockout (GKO) mice. In 3T3-L1 adipocytes treated with an SCD inhibitor, basal glucose uptake and the cellular expression of GLUT1 were significantly increased while GLUT4 expression remained unchanged. Consistently, adipose-specific SCD1 knockout (AKO) mice had significantly elevated GLUT1 expression, but not GLUT4, in white adipose tissue compared to Lox counterparts. Concurrently, adiponectin expression was significantly diminished, whereas TNF-alpha expression was elevated. In contrast, in adipose tissue of GKO mice, GLUT4 and adiponectin expression were significantly elevated with lowered TNF-alpha expression and little change in GLUT1 expression, suggesting a differential responsiveness of adipose tissue to global- or adipose-specific SCD1 deletion. Taken together, these results indicate that adipose-specific deletion of SCD1 induces GLUT1 up-regulation in adipose tissue, associated with decreased adiponectin and increased TNF-alpha production, and suggest that GLUT1 may play a critical role in controlling glucose homeostasis of adipose tissue in adipose-specific SCD1-deficient conditions.
Hyun
Chang-Kee
CK
School of Life Science, Handong Global University, Pohang, Kyungbuk 791-708, Republic of Korea.
Kim
Eun-Do
ED
Flowers
Matthew T
MT
Liu
Xueqing
X
Kim
Eunha
E
Strable
Maggie
M
Ntambi
James M
JM
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology; D052242:Adiponectin / Q000096:biosynthesis; D052436:Adipose Tissue, White / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017353:Gene Deletion; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D013230:Stearoyl-CoA Desaturase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation
D052242:Adiponectin; D004791:Enzyme Inhibitors; D051272:Glucose Transporter Type 1; D014409:Tumor Necrosis Factor-alpha; C490333:Scd1 protein, mouse; D013230:Stearoyl-CoA Desaturase