Adipogenic differentiation of human adipose tissue-derived stem cells obtained from cryopreserved adipose aspirates.
BACKGROUND
Although frozen adipose tissue is frequently used for soft tissue augmentation, the viability of frozen fat remains a controversy. The cryopreservation of adipose tissue is important for the future use of adipose-derived stem cells (ASCs) and adipocytes.


OBJECTIVE
To determine whether optimal cryopreservation techniques with regard to the addition of cryopreservative agents and preservation temperature is essential for the long-term storage of adipose tissue and whether ASCs from cryopreserved adipose aspirates are reliable for use in adipogenic differentiation.


METHODS
Adipose tissue was frozen directly or with cryoprotectant at -20 degrees C or -80 degrees C for 1 year. The viability of adipose aspirates and the differentiation of ASCs isolated from adipose tissue were evaluated.


RESULTS
The viability of adipose aspirates frozen with dimethyl sulfoxide at -80 degrees C was approximately 87% after 2 months of storage. Moreover, ASCs from adipose tissue stored with cryoprotectant survived successfully for 1 year and differentiated into adipocytes, although ASCs were not detected in the directly frozen adipose tissue.


CONCLUSIONS
Adipose tissue cryopreserved with cryoprotectant and stored at optimal temperature might prove to be a reliable source of human ASCs and adipocytes.
Lee
Jung Eun
JE
Anterogen Co. Ltd., Seoul, Korea.
Kim
Inok
I
Kim
Mihyung
M
D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000502:physiology*; D053687:Adult Stem Cells / Q000166:cytology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005615:Freezing; D006801:Humans; D015187:Lipectomy; D050151:Subcutaneous Fat / Q000166:cytology*; D046509:Tissue Culture Techniques; D014022:Tissue Survival
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide