title: A novel biomimetic composite scaffold hybridized with mesenchymal stem cells in repair of rat bone defects models.
abstract: In this study, the in vivo bone-regenerative potential of a novel bioactive glass-collagen-hyaluronic acid-Phosphatidylserine (BG-COL-HYA-PS) composite scaffold hybridized with mesenchymal stem cells (MSCs) was investigated in a rat bone defect model. HrGFP-labeled MSCs were cultured for 2 weeks on the BG-COL-HYA-PS scaffold before implantation into the defect. A cell-free scaffold and an untreated defect were used as controls. The regeneration process was evaluated by histology, X-ray, and mechanical rigidity experiments at different time points post-implantation. The results revealed that BG-COL-HYA-PS scaffold exhibited a low inflammatory response and foreign body response within 3 weeks. At week 6, those responses disappeared following the resorption of scaffolds and the formation of new bone. Compared with the pure scaffold or empty group, the introduction of MSCs into the porous scaffold dramatically enhanced the efficiency of the new bone formation and biomechanical property of the femur. In addition, the transplanted MSCs could survive for up to 3 weeks or longer. The results demonstrated that the BG-COL-HYA-PS scaffold was biocompatible and osteoconductive and the transplanted MSCs with the scaffold enhanced the healing of the bone defect.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, SunYat-sen University, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Guangzhou, Guangdong 510080, People's Republic of China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Xu
authors.forename: Peiqiang
authors.initials: P
authors.lastname: Su
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Meng
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
authors.forename: Xuhui
authors.initials: X
authors.lastname: Yang
authors.forename: Weihua
authors.initials: W
authors.lastname: Yu
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D032701:Biomimetics; D001861:Bone Regeneration / Q000502:physiology; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D005260:Female; D005269:Femur / Q000473:pathology*; D006820:Hyaluronic Acid / Q000737:chemistry; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C022107:Bioglass; D006820:Hyaluronic Acid; D003094:Collagen