Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2.
Cyclooxygenase (COX) enzymes play important roles in normal physiology and during inflammation of various cells and tissues. In order to help understand the functions of these enzymes, their genes can be cloned to facilitate the production of the proteins in recombinant form. We outline a method to clone the genes from a human macrophage cell line for expression in an insect cell line infected with recombinant baculovirus encoding these enzymes.
Staten
Nicholas R
NR
Pfizer, Inc., Chesterfield, MO, USA.
Reitz
Beverly A
BA
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D051545:Cyclooxygenase 1 / Q000235:genetics* / Q000302:isolation & purification; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000302:isolation & purification; D005822:Genetic Vectors; D006801:Humans; D007313:Insecta / Q000166:cytology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification
D012333:RNA, Messenger; D011994:Recombinant Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2