Flavitalea populi gen. nov., sp. nov., isolated from soil of a Euphrates poplar (Populus euphratica) forest.
A novel strain, designated HY-50R(T), isolated from soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China, was characterized using a polyphasic taxonomic approach. Cells of the isolate were gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum Bacteroidetes, its closest relatives being Niastella populi THYL-44(T) (93.6 % similarity), Flavisolibacter ginsengisoli Gsoil 643(T) (93.5 %), Terrimonas ferruginea IAM 15098(T) (93.3 %) and Flavisolibacter ginsengiterrae Gsoil 492(T) (93.2 %). The major fatty acids were iso-C(15 : 1) G (11.7 %), iso-C(15 : 0) (19.6 %) and iso-C(17 : 0) 3-OH (19.3 %). The predominant menaquinone of strain HY-50R(T) was MK-7 and the genomic DNA G+C content was 46.8 mol%. Flexirubin-type pigments were not produced. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis, strain HY-50R(T) represents a novel species of a novel genus, for which the name Flavitalea populi gen. nov., sp. nov. is proposed. The type strain is HY-50R(T) ( = CCTCC AB 208255(T)  = NRRL B-59222(T)).
Wang
Yang
Y
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Cai
Feng
F
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Tang
Yali
Y
Institute of Hydrobiology, Jinan University, Guangzhou 510632, PR China.
Dai
Jun
J
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Qi
Huan
H
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Rahman
Erkin
E
College of Life Science and Technology, Xinjiang University, Urumchi 830046, PR China.
Peng
Fang
F
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Fang
Chengxiang
C
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D008969:Molecular Sequence Data; D010802:Phylogeny; D032107:Populus; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7