Heavy metal tolerant Metalliresistens boonkerdii gen. nov., sp. nov., a new genus in the family Bradyrhizobiaceae isolated from soil in Thailand.
Bacterial strains from inoculated soybean field soil in Thailand were directly isolated using Bradyrhizobium japonicum selective medium (BJSM), on the basis of Zn(2+) and Co(2+) resistance of B. japonicum and B. elkanii. The isolates were classified into symbiotic and non-symbiotic groups by inoculation assays and Southern hybridization of nod and nif genes. In this study, a nearly full-length 16S rRNA gene sequence showed that the non-symbiotic isolates were more closely related to members of Rhodopseudomonas and to a number of uncultured bacterial clones than to members of Bradyrhizobium. Therefore, a polyphasic study was performed to determine the taxonomic positions of four representatives of the non-symbiotic isolates. Multilocus phylogenetic analysis of individual genes and a combination of the 16S rRNA and three housekeeping genes (atpD, recA and glnII) supported the placement of the non-symbiotic isolates in a different genus. The ability of heavy metal resistance in conjunction with phenotypic analyses, including cellular fatty acid content and biochemical characteristics, showed that the non-symbiotic isolates were differentiated from the other related genera in the family Bradyrhizobiaceae. Therefore, the non-symbiotic isolates represented a novel genus and species, for which the name Metalliresistens boonkerdii gen. nov., sp. nov. is proposed. The type strain is NS23 (= NBRC 106595(T)=BCC 40155(T)).
Noisangiam
Rujirek
R
Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, Nakhonrachasima 30000, Thailand.
Nuntagij
Achara
A
Pongsilp
Neelawan
N
Boonkerd
Nantakorn
N
Denduangboripant
Jessada
J
Ronson
Clive
C
Teaumroong
Neung
N
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D041841:Bradyrhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000737:chemistry; D020459:Genes, rRNA; D019216:Metals, Heavy / Q000494:pharmacology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology; D013045:Species Specificity; D013559:Symbiosis; D013785:Thailand; D015032:Zinc / Q000494:pharmacology
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D003035:Cobalt; D015032:Zinc