title: Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae).
abstract: Proteases play a vital role in both the life cycle of parasites and the parasite-host relationship and are considered important virulence factors. In the present study, the presence of proteases with collagenolytic activity was investigated in the fish nematode Hysterothylacium aduncum during in vitro development. Collagenolytic activity was found in all studied developmental stages of the nematode (third [L3] and fourth [L4] larval stages and adults). In L3, the activity was maximum at pH 6.5 and, in the other stages, at 7.0. Pepsin is known to favour in vitro development of the worm, but, in this study, collagenolytic activity was shown to be significantly greater when no pepsin was added to the culture medium (at pH 6.5, p = 0.011). At pH 7.0, most activity was observed in the immature adult, after the final moult, suggesting that the collagenolytic activity may be involved in remodelling of the cuticle and in sexual maturity. On the other hand, at pH 6.5, activity may be related to tissue migration by L3 within the host. Using specific inhibitors, it was demonstrated that most of the collagenolytic activity detected in all the developmental stages was due to metalloproteases (40 to 100%), although serine proteases were also detected in L4 and adults (10 to 30%).
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Campus de Cartuja, Universidad de Granada, 18071 Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Malagón
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Adroher
authors.forename: Manuel
authors.initials: M
authors.lastname: Díaz-López
authors.forename: Rocío
authors.initials: R
authors.lastname: Benítez
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism*; D009348:Nematoda / Q000201:enzymology*; D057057:Serine Proteases / Q000235:genetics / Q000378:metabolism*
chemical_list: D003094:Collagen; D045726:Metalloproteases; D057057:Serine Proteases