title: Identification and removal of colanic acid from plasmid DNA preparations: implications for gene therapy.
abstract: Polysaccharide contaminants in plasmid DNA, including current good manufacturing practices (cGMP) clinical preparations, must be removed to provide the greatest safety and efficacy for use in gene therapy and other clinical applications. We developed assays and methods for the detection and removal of these polysaccharides, our Super Clean DNA (SC-DNA) process, and have shown that these contaminants in plasmid DNA preparations are responsible for toxicity observed post-injection in animals. Furthermore, these contaminants limit the efficacy of low and high doses of plasmid DNA administered by numerous delivery routes. In particular, colanic acid (CA) that is mainly long-chained, branched and has high molecular weight (MW) is most refractory when complexed to cationic delivery vehicles and injected intravenously (IV). Because CA is often extremely large and tightly intertwined with DNA, it must be degraded, in order, to be effectively removed. We have produced a recombinant, truncated colanic acid degrading enzyme (CAE) that successfully accomplishes this task. Initially, we isolated a newly identified CAE from a bacteriophage that required truncation for proper folding while retaining its full enzymatic activity during production. Any plasmid DNA preparation can be digested with CAE and further purified, providing a critical advance to non-viral gene therapy.
authors.affiliation: Department of Surgery, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: P
authors.initials: P
authors.lastname: Firozi
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: F A
authors.initials: FA
authors.lastname: Quiocho
authors.forename: K C
authors.initials: KC
authors.lastname: Worley
authors.forename: N S
authors.initials: NS
authors.lastname: Templeton
mesh_terms: D000818:Animals; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D005452:Fluoresceins / Q000737:chemistry; D005643:Fucose / Q000737:chemistry; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D008969:Molecular Sequence Data; D010957:Plasmids / Q000302:isolation & purification*; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014574:Uronic Acids / Q000032:analysis
chemical_list: D004798:Enzymes; D005452:Fluoresceins; D011134:Polysaccharides; D011994:Recombinant Proteins; D014574:Uronic Acids; C039083:5-((4,6-dichloro-1,3,5-triazin-2-yl)amino)fluorescein; D005643:Fucose; C004275:colanic acid