Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.
The Mpk1 MAP kinase of the cell wall integrity (CWI) signalling pathway induces transcription of the FKS2 gene in response to cell wall stress through a non-catalytic mechanism that involves stable association of Mpk1 with the Swi4 transcription factor. This dimeric complex binds to a Swi4 recognition site in the FKS2 promoter. The Swi6 transcription factor is also required to bind this ternary complex for transcription initiation to ensue. In this context, the Mlp1 pseudokinase serves a redundant function with Mpk1. We have identified three additional genes, CHA1, YLR042c and YKR013w, that are induced by cell wall stress through the same mechanism. We report on the behaviour of several promoter-lacZ reporter plasmids designed to detect cell wall stress transcription through this pathway.
Kim
Ki-Young
KY
Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA.
Levin
David E
DE
D019677:Artificial Gene Fusion; D002473:Cell Wall / Q000378:metabolism / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; C080988:MLP1 protein, S cerevisiae; D008565:Membrane Proteins; D009687:Nuclear Proteins; C095462:PRY2 protein, S cerevisiae; D016601:RNA-Binding Proteins; C081540:SBF protein, S cerevisiae; C062214:SWI4 protein, S cerevisiae; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005964:Glucosyltransferases; C095724:GSC2 protein, S cerevisiae; D001616:beta-Galactosidase