[Significance of K-ras detection in colorectal cancer].
OBJECTIVE
To determine the mutation status of K-ras gene in colorectal cancer and analyze the associations between its mutation status and clinicopathological characteristics in colorectal cancer so as to select the patients likely to benefit from a targeted therapy.


METHODS
A total of 208 colorectal cancer tissue samples were collected from September 2008 to February 2009. DNA was extracted with a genomic DNA miniprep kit. Then PCR was performed with the designed primers and the product directly sequenced by the Sanger method. Then the associations between K-ras mutation status and clinicopathological characteristics in colorectal cancer were analyzed.


RESULTS
Of 208 cases, 91 cases of K-ras gene mutation were detected. The 12 or 13 codon had a mutation rate of 43.8%. There were no significant differences in gender, tumor location, histopathological grading and Duke's stage between the wild and mutated groups.


CONCLUSIONS
Detection of K-ras gene status in colorectal cancer will help to select the patients likely to benefit from the monoclonal antibody therapy of targeting epidermal growth factor receptor.
Xu
Xiang-ming
XM
Colorectal Diseases Center of Zhejiang Province, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. xuxiangming1974@msn.com
Zhang
Guo-liang
GL
Li
Min-wei
MW
Wang
Dan-yang
DY
Lin
Jian-jiang
JJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged