title: Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China.
abstract: One hundred Mycoplasma pneumoniae strains were isolated from pediatric patients from March 2008 to July 2009. Of 100 isolates, 90 (90%) were resistant to erythromycin (MICs >128 microg/mL for 88 strains and 64 microg/mL for 2 strains), azithromycin, and clarithromycin. Fluoroquinolones and tetracyclines maintain good activities against clinical M. pneumoniae isolates. Of 90 macrolide-resistant M. pneumoniae strains, 88 (98%) harbored an A-to-G transition mutation at position 2063 in 23S rRNA genes, and the remaining 2 showed either A2064G or A2063T mutation; the latter point mutation is newly discovered and reported. Ninety-three (93%) clinical isolates were classified into the P1 gene restriction fragment length polymorphism (RFLP) type I, and 7 (7%) were type II.
authors.affiliation: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xinyu
authors.initials: X
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Xu
authors.forename: Wanhua
authors.initials: W
authors.lastname: Li
authors.forename: Demei
authors.initials: D
authors.lastname: Zhu
authors.forename: Minggui
authors.initials: M
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D002681:China; D017291:Clarithromycin / Q000494:pharmacology; D016172:DNA Fingerprinting; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D024841:Fluoroquinolones / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D011019:Pneumonia, Mycoplasma / Q000382:microbiology*; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D018942:Macrolides; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D013754:Tetracyclines; D004917:Erythromycin; D017963:Azithromycin; D017291:Clarithromycin