Development of fluoroapatite chromatography for the purification of monoclonal antibody.
Monoclonal antibody was purified from tissue culture fluid using ceramic fluoroapatite chromatography. Efficiency of the capture step was shown to be sensitive to pH and phosphate concentration. More than 90% of host cell proteins were removed by ceramic fluoroapatite chromatography. Studies regarding pH control and metal adsorption are presented.
Ng
Paul K
PK
Process R&D Applications, Life Science Group, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com
Yoshitake
Tomohiko
T
Snyder
Mark A
MA
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001031:Apatites / Q000737:chemistry*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry
D000911:Antibodies, Monoclonal; D001031:Apatites; D007074:Immunoglobulin G; D008670:Metals; C025105:fluorapatite