title: [Prokaryotic expression and immunologic identification of P-29 gene from Echinococcus granulosus hydatid cyst].
abstract: OBJECTIVE
  To clone and express P-29 gene of Echinococcus granulosus, and analyze its immunoreactivity.
  
  
  METHODS
  Total RNA was extracted from the hydatid cyst of E. granulosus and its P-29 gene was amplified by RT-PCR. The PCR product was cloned into pET44a(+) vector. The recombinant plasmid was transformed into E. coli BL21 (DE3) and followed by expression of the protein induced by IPTG. The protein was purified, and tested by SDS-PAGE. Its immunoreactivity was examined by Western blotting.
  
  
  RESULTS
  The recombinant expression plasmid was identified by PCR, double endonuclease digestion and sequencing. The recombinant Nus-P-29 was about Mr 93 000 with a concentration of 0.78 mg/ml. It was recognized by the sera of cystic echinococcosis patients.
  
  
  CONCLUSIONS
  The P-29 gene has been expressed with adequate immunoreactivity.
authors.affiliation: College of Life Science, Inner Mongolia University, Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Hohhot 010021, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Xi-yan
authors.initials: XY
authors.lastname: Hao
authors.forename: Xian-wei
authors.initials: XW
authors.lastname: Chen
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Shu-yu
authors.initials: SY
authors.lastname: Li
authors.forename: Jiao-fu
authors.initials: JF
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology*; D003001:Cloning, Molecular; D048209:Echinococcus granulosus / Q000276:immunology*; D017238:Genes, Helminth; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D011994:Recombinant Proteins