title: Assessment of GABARAP self-association by its diffusion properties.
abstract: Gamma-aminobutyric acid type A receptor-associated protein (GABARAP) belongs to a family of small ubiquitin-like adaptor proteins implicated in intracellular vesicle trafficking and autophagy. We have used diffusion-ordered nuclear magnetic resonance spectroscopy to study the temperature and concentration dependence of the diffusion properties of GABARAP. Our data suggest the presence of distinct conformational states and provide support for self-association of GABARAP molecules. Assuming a monomer-dimer equilibrium, a temperature-dependent dissociation constant could be derived. Based on a temperature series of (1)H(15)N heteronuclear single quantum coherence nuclear magnetic resonance spectra, we propose residues potentially involved in GABARAP self-interaction. The possible biological significance of these observations is discussed with respect to alternative scenarios of oligomerization.
authors.affiliation: Institut für Strukturbiologie und Biophysik 3, Forschungszentrum Jülich, 52425 Jülich, Germany.
authors.forename: Victor
authors.initials: V
authors.lastname: Pacheco
authors.forename: Peixiang
authors.initials: P
authors.lastname: Ma
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Thielmann
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hartmann
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Weiergräber
authors.forename: Jeannine
authors.initials: J
authors.lastname: Mohrlüder
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins; D004058:Diffusion; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D055503:Protein Multimerization; D013696:Temperature
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C116741:GABARAP protein, human; D008869:Microtubule-Associated Proteins