Basal and induced granulopoiesis in outbred, F1 hybrid and inbred mice: can inbreeding depression influence the experimental practice?
In this study we examined differences in selected indices of granulopoiesis in outbred, F(1) hybrid and inbred mouse strains. Specifically, serum granulocyte colony-stimulating factor (G-CSF) levels, numbers of marrow granulocyte-macrophage progenitor cells and morphologically recognizable proliferative marrow granulocytic precursor cells were evaluated. These parameters were determined in untreated controls, and in mice exposed either to a non-specific stimulus (injection of saline) or to a granulopoiesis-enhancing stimulus (administration of a cyclooxygenase-2 inhibitor, meloxicam). Lower levels of G-CSF were detectable in the outbred ICR mice, which also demonstrated an enhanced response to both types of the stimuli. Considering the fact that outbred mice are closer to natural mammalian populations, including human ones, the possibility of using outbred mice, instead of the often used inbred strains, for experiments evaluating the effects of pharmacological interventions on hematopoiesis should be investigated.
Hofer
Michal
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic. hofer@ibp.cz
Pospísil
Milan
M
Dusek
Ladislav
L
Holá
Jirina
J
Hoferová
Zuzana
Z
Weiterová
Lenka
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D055014:Granulocyte-Macrophage Progenitor Cells; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006824:Hybridization, Genetic / Q000235:genetics*; D007178:Inbreeding; D007274:Injections, Intraperitoneal; D008297:Male; D000077239:Meloxicam; D051379:Mice; D008815:Mice, Inbred Strains; D038042:Myelopoiesis / Q000187:drug effects / Q000502:physiology*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology; D013843:Thiazines / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D016861:Cyclooxygenase Inhibitors; D013843:Thiazines; D013844:Thiazoles; D016179:Granulocyte Colony-Stimulating Factor; D012965:Sodium Chloride; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000077239:Meloxicam