Functional characterization of the PP2C phosphatase CaPtc2p in the human fungal pathogen Candida albicans.
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ion. There are seven PP2C-like genes in Candida albicans. In this study, we demonstrate that CaPtc2p is a PP2C phosphatase. Surprisingly, in addition to the cytoplasmic localization, CaPtc2p is partially associated with mitochondria in yeast-form and filamentous cells of C. albicans. Expression of CaPTC2 is developmentally regulated during the serum-induced filamentation. Deletion of CaPTC2 renders C. albicans cells sensitive to SDS and azole antifungals, as well as the DNA methylation agent methylmethane sulphonate and the DNA synthesis inhibitor hydroxyurea. Therefore, CaPtc2p might fulfil multiple functions, including the regulation of mitochondrial physiology and checkpoint recovery from DNA damage in C. albicans cells.
Feng
Jinrong
J
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
Zhao
Jingwen
J
Li
Jing
J
Zhang
Lilin
L
Jiang
Linghuo
L
D000935:Antifungal Agents / Q000633:toxicity; D001393:Azoles / Q000633:toxicity; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D003593:Cytoplasm / Q000201:enzymology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006801:Humans; D006918:Hydroxyurea / Q000633:toxicity; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008928:Mitochondria / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000172:deficiency / Q000378:metabolism*; D010802:Phylogeny; D000071636:Protein Phosphatase 2C; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity
D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D012967:Sodium Dodecyl Sulfate; D008741:Methyl Methanesulfonate; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D006918:Hydroxyurea