Prevention by L-carnitine of DNA damage induced by propionic and L-methylmalonic acids in human peripheral leukocytes in vitro.
Propionic acidemia (PAemia) and methylmalonic acidemia (MMAemia) are inborn errors of propionate metabolism characterized by the accumulation of, respectively, propionic and l-methylmalonic acids (and their metabolites) in the blood and tissues of affected patients. The conditions lead to severe metabolic complications in the neonatal period and to long-term neurological manifestations. Treatment for these disorders consists of a protein-restricted diet, supplemented with synthetic formulas of amino acids, but excluding isoleucine, threonine, valine and methionine; and l-carnitine, to promote detoxication. In vitro and in vivo studies have demonstrated that lipid and protein oxidative damage may be involved in the pathophysiology of these diseases, but DNA damage has not been fully investigated. In this work, we evaluated in vitro the effects of PA and MMA, in the presence or absence of l-carnitine, on DNA damage in peripheral leukocytes, as determined by the alkaline comet assay, using silver staining and visual scoring. PA and MMA induced a DNA damage index (DI) significantly higher than that of the control group. l-Carnitine significantly reduced PA- and MMA-induced DNA damage, in a concentration-dependent manner. Our findings indicate that PA and MMA induce DNA damage and l-carnitine is able to prevent this damage.
Ribas
Graziela S
GS
Programa de Pós-Graduação em Ciências Farmacêuticas, UFRGS, Av. Ipiranga 2752, Porto Alegre, RS, Brazil. grazischmitt@terra.com.br
Manfredini
Vanusa
V
de Marco
Maria Gilda
MG
Vieira
Rosana B
RB
Wayhs
Carlos Y
CY
Vanzin
Camila S
CS
Biancini
Giovana B
GB
Wajner
Moacir
M
Vargas
Carmen R
CR
D002331:Carnitine / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008764:Methylmalonic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity; D011422:Propionates / Q000633:toxicity*
D009153:Mutagens; D011422:Propionates; D008764:Methylmalonic Acid; C029658:propionic acid; D002331:Carnitine