title: Variation in the expression of Hsp27, αB-crystallin mRNA and protein in heart and liver of pigs exposed to different transport times.
abstract: Twenty pigs were randomly divided into four groups of five pigs each (not transported - control, 1, 2 and 4h of transportation). A significant increase of ALT, AST and CK in the blood serum and acute parenchyma cell lesions were observed and those were characterized by acute degenerations in the heart and liver. Hsp27 expression levels increased significantly in the heart after 2h and in the liver after 4h of transportation, accompanying with the hsp27 mRNA increasing significantly in the heart and liver after 1h of transportation. αB-crystallin expression levels were fluctuant (not significantly) in the heart and liver during transporting, however, αB-crystallin mRNA increase notably in the heart after 1h and decrease significantly in the liver at 1 and 2h of transportation, respectively. In conclusion, the cellular damage to the heart and liver is highest after 1h of transportation, Hsp27 and αB-crystallin play dissimilar roles and show tissue-specific response in different tissues during transportation.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Lingxiang
authors.initials: L
authors.lastname: Xin
authors.forename: Endong
authors.initials: E
authors.lastname: Bao
authors.forename: Jörg
authors.initials: J
authors.lastname: Hartung
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yue
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006321:Heart / Q000502:physiology; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D014186:Transportation; D038203:alpha-Crystallin B Chain / Q000378:metabolism* / Q000502:physiology
chemical_list: D055551:HSP27 Heat-Shock Proteins; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase