title: Antioxidant activity of oligosaccharide ester extracted from Polygala tenuifolia roots in senescence-accelerated mice.
abstract: The constituents of the ethanol extract from the root of Polygala tenuifolia Willd. (Polygalaceae) were investigated for antioxidant activity in senescence-accelerated mice. Consequently, two relevant samples were obtained, a fraction separated by macroporous resin (YZ-OE), and a major pure crystal of 3,6'-disinapoyl sucrose (DISS). Based on HPLC-ESI-MS analysis, the most constituents in the YZ-OE fraction from the extract of P. tenuifolia were oligosaccharide esters. The antioxidant activities of these two samples were evaluated using the accelerated senescence-prone, short-lived mice (SAMP) in vivo. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were increased significantly in SAMP mice fed oligosaccharide esters (YZ-OE 50 mg/kg) and its constituents (DISS 50 mg/kg). However, the content of malondialdehyde (MDA) was increased in the blood and liver of SAMP mice. But when given YZ-OE, it could be decreased, by 44.3% and 47.5%, respectively, compared with the SAMP model. Results from the analyses indicated that the oligosaccharide esters (YZ-OE) from roots of P. tenuifolia had a high in vivo antioxidant activity.
authors.affiliation: Department of Clinical Pharmacology, Pharmacy Care Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Dai-Hong
authors.initials: DH
authors.lastname: Guo
authors.forename: Bao-Rong
authors.initials: BR
authors.lastname: Lu
authors.forename: Khalid
authors.initials: K
authors.lastname: Rahman
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mu
authors.forename: Dong-Xiao
authors.initials: DX
authors.lastname: Wang
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D004952:Esters; D005979:Glutathione Peroxidase / Q000097:blood; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D009844:Oligosaccharides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D010936:Plant Extracts / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots; D031801:Polygala; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D004952:Esters; D009844:Oligosaccharides; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase