title: Ultra-fast adenosine 5'-triphosphate, adenosine 5'-diphosphate and adenosine 5'-monophosphate detection by pressure-assisted capillary electrophoresis UV detection.
abstract: Herein, we report a new CE method to measure adenine nucleotides adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in red blood cells. For this purpose, 20 mmol/L sodium acetate buffer at pH 3.80 was used as running electrolyte, and the separation was performed by the simultaneous application of a CE voltage of 25 kV and an overimposed pressure of 0.2 psi from inlet to outlet. A rapid separation of these analytes in less than 1.5 min was obtained with a good reproducibility for intra- and inter-assay (CV<4 and 8%, respectively) and an excellent analytical recovery (from 98.3 to 99%). The applicability of our method was proved by measuring adenine nucleotides in red blood cells.
authors.affiliation: Department of Biomedical Sciences and Centre of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy. angelozinellu@libero.it
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.forename: Bastianina
authors.initials: B
authors.lastname: Scanu
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Pisanu
authors.forename: Manuela
authors.initials: M
authors.lastname: Sanna
authors.forename: Maria
authors.initials: M
authors.lastname: Franca Usai
authors.forename: Luca
authors.initials: L
authors.lastname: Deiana
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood / Q000302:isolation & purification*; D000249:Adenosine Monophosphate / Q000097:blood / Q000302:isolation & purification*; D000255:Adenosine Triphosphate / Q000097:blood / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D011312:Pressure; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate