In vitro cyclooxygenase activity assay in tissue homogenates.
The use of in vitro cyclooxygenase (COX) activity assays has been particularly useful for comparing the effect of different drugs on COX activity in different tissues. In addition, this assay is relatively quick, cheap, and a large number of samples can be tested at the same time. However, one limitation of this assay is the fact that it does not discriminate between the activities of different COX isoforms.
Ayoub
Samir S
SS
Centre for Biochemical Pharmacology, Experimental Pathology Group, St. Bart's and the London School of Medicine and Dentistry, William Harvey Research Institute, Queen Mary University of London, London, UK.
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods*; D004837:Epinephrine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013048:Specimen Handling
D016861:Cyclooxygenase Inhibitors; D016718:Arachidonic Acid; D011451:Prostaglandin-Endoperoxide Synthases; D005978:Glutathione; D004837:Epinephrine