Determination of sulfate ester content in sulfated oligo- and poly-saccharides by capillary electrophoresis with indirect UV detection.
Carbohydrates having sulfate groups such as glycosaminoglycans and chemically synthesized sucrose sulfate show interesting and important biological activities. We adapted CE with indirect UV detection technique to the determination of sulfate ester in sulfated carbohydrates, which were previously hydrolyzed with HCl. The liberated sulfate ion was analyzed using a background electrolyte consisting of triethanolamine-buffered chromate with hexamethonium bromide. Sulfate contents of glucose 3-sulfate and sucrose octasulfate used as a model were in good agreement with theoretical values (accuracy, 95.9-96.7 and 97.4-101.9%, respectively), and relative standard deviation values run-to-run were 0.977 and 1.90%, respectively. We applied the method to the determination of the sulfate contents of some glycosaminoglycan samples and showed that the contents were in good agreement with those calculated from sulfur content.
Kinoshita
Mitsuhiro
M
Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashi-osaka, Japan.
Kakoi
Naotaka
N
Matsuno
Yu-Ki
YK
Hayakawa
Takao
T
Kakehi
Kazuaki
K
D019075:Electrophoresis, Capillary / Q000379:methods*; D006025:Glycosaminoglycans / Q000737:chemistry*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013431:Sulfates / Q000032:analysis*
D006025:Glycosaminoglycans; D013431:Sulfates