Augmentation of cholinesterases and ATPase activities in the cerebellum and pons-medulla oblongata, by a combination of antioxidants (resveratrol, ascorbic acid, alpha-lipoic acid and vitamin E), in acutely lindane intoxicated mice.
In the present investigation neurotoxic effects of lindane and the protective potential of a combination of antioxidants against lindane-induced toxicity were evaluated in Swiss mice. The investigation was carried out on acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and adenosine triphosphatase (ATPase) activities of the cerebellum and pons-medulla oblongata. Healthy mice, 7-8 weeks old were administered acute dose of lindane (40 mg/kg b.w.), antioxidants, both lindane and antioxidants, and vehicle in four separate groups, subcutaneously. Resveratrol (Res), ascorbic acid (C), alpha-lipoic acid (ALA) and vitamin E (E) were used in the combination for neuroprotection at the concentration of 5 mg/kg b.w., 50 mg/kg b.w., 20 mg/kg b.w. and 50 mg/kg b.w. respectively. Enzymatic activities were used as biochemical marker for manifestation of lindane-induced acute toxicity. Protective effects of antioxidants were also evaluated using the same parameters. Treatment of lindane to normal control animals resulted in a significant decrease in AChE, BChE and ATPase levels in crude homogenates of cerebellum and pons-medulla. Antioxidants treatment significantly increased the levels of enzymes. Critical difference (CD) of AChE, BChE and ATPase levels in various groups was found significant at 1% in cerebellum and pons-medulla both (i.e. P<0.01).
Bist
Renu
R
Department of Bioscience and Biotechnology, Banasthali University, Banasthali (Raj.)-304022, India. renu_bisht22@yahoo.co.in
Bhatt
Devendra Kumar
DK
D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects* / Q000201:enzymology*; D002802:Cholinesterases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D001556:Hexachlorocyclohexane / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D007306:Insecticides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects* / Q000201:enzymology*; D051379:Mice; D011149:Pons / Q000187:drug effects* / Q000201:enzymology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D008063:Thioctic Acid / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D007306:Insecticides; D013267:Stilbenes; D014810:Vitamin E; D001556:Hexachlorocyclohexane; D008063:Thioctic Acid; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000251:Adenosine Triphosphatases; D001205:Ascorbic Acid; D000077185:Resveratrol