Quantification of enhanced osteoblastic adhesion to ultraviolet-treated titanium plate.
Although the advantage of ultraviolet (UV) irradiation of titanium plates for the attachment of osteoblast is known, the details of the experimental conditions have not been described in previous literature. We established optimal conditions of UV irradiation of titanium plate for the adhesion of mouse osteoblast MC3T3-E1 cells. The viable cell number was determined by MTT method. UV irradiation at two different wavelengths (253.7 and 365 nm) enhanced the cell attachment on titanium plate to comparable extents. The optimal UV exposure duration was 20 minutes and prolonged irradiation slightly reduced cell attachment. The attached cells proliferated during 24 hours, accompanied by the enhanced consumption of extracellular glutamine and arginine. The present study supports the previous reports of the efficacy of UV irradiation, and this simple and rapid assay system may be applicable for the study of the interaction of osteoblast and UV-activated titanium plates.
Contreras
René García
RG
Dental Research Center (CIEAO), Faculty of Dentistry, Autonomous University of State of Mexico (UAEM), Toluca, Estado de México, México.
Adachi
Kazunori
K
Yokote
Yoshiko
Y
Sakagami
Hiroshi
H
Hibino
Yasushi
Y
Nakajima
Hiroshi
H
Shimada
Jun
J
D016475:3T3 Cells / Q000166:cytology / Q000528:radiation effects*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002448:Cell Adhesion / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D005973:Glutamine / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000528:radiation effects*; D013499:Surface Properties; D014025:Titanium / Q000528:radiation effects*; D014466:Ultraviolet Rays
D005973:Glutamine; D001120:Arginine; D014025:Titanium