title: Enhanced degradation of phenol by Pseudomonas sp. CP4 entrapped in agar and calcium alginate beads in batch and continuous processes.
abstract: Phenol is one of the major toxic pollutants in the wastes generated by a number of industries and needs to be eliminated before their discharge. Although microbial degradation is a preferred method of waste treatment for phenol removal, the general inability of the degrading strains to tolerate higher substrate concentrations has been a bottleneck. Immobilization of the microorganism in suitable matrices has been shown to circumvent this problem to some extent. In this study, cells of Pseudomonas sp. CP4, a laboratory isolate that degrades phenol, cresols, and other aromatics, were immobilized by entrapment in Ca-alginate and agar gel beads, separately and their performance in a fluidized bed bioreactor was compared. In batch runs, with an aeration rate of 1 vol(-1) vol(-1) min(-1), at 30°C and pH 7.0 ± 0.2, agar-encapsulated cells degraded up to 3000 mg l(-1) of phenol as compared to 1500 mg l(-1) by Ca-alginate-entrapped cells whereas free cells could tolerate only 1000 mg l(-1). In a continuous process with Ca-alginate entrapped cells a degradation rate of 200 mg phenol l(-1) h(-1) was obtained while agar-entrapped cells were far superior and could withstand and degrade up to 4000 mg phenol l(-1) in the feed with a maximum degradation rate of 400 mg phenol l(-1) h(-1). The results indicate a clear possibility of development of an efficient treatment technology for phenol containing waste waters with the agar-entrapped bacterial strain, Pseudomonas sp. CP4.
authors.affiliation: Department of Food Microbiology, Central Food Technological Research Institute, Mysore, 570 013, India.
authors.forename: P Y
authors.initials: PY
authors.lastname: Aneez Ahamad
authors.forename: A A
authors.initials: AA
authors.lastname: Mohammad Kunhi
mesh_terms: D000362:Agar; D000464:Alginates; D001673:Biodegradation, Environmental; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D019800:Phenol / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D019800:Phenol; D020723:Glucuronic Acid; D000362:Agar