title: Real-time assessment of renal cortical microvascular perfusion heterogeneities using near-infrared laser speckle imaging.
abstract: Laser speckle imaging (LSI) is able to provide full-field perfusion maps of the renal cortex and allows quantification of the average LSI perfusion within an arbitrarily set region of interest and the recovery of LSI perfusion histograms within this region. The aim of the present study was to evaluate the use of LSI for mapping renal cortical microvascular perfusion and to demonstrate the capability of LSI to assess renal perfusion heterogeneities. The main findings were that: 1) full-field LSI measurements of renal microvascular perfusion were highly correlated to single-point LDV measurements; 2) LSI is able to detect differences in reperfusion dynamics following different durations of ischemia; and 3) renal microvascular perfusion heterogeneities can be quantitatively assessed by recovering LSI perfusion histograms.
authors.affiliation: Department of Translational Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. R.Bezemer@amc.uva.nl
authors.forename: Rick
authors.initials: R
authors.lastname: Bezemer
authors.forename: Matthieu
authors.initials: M
authors.lastname: Legrand
authors.forename: Eva
authors.initials: E
authors.lastname: Klijn
authors.forename: Michal
authors.initials: M
authors.lastname: Heger
authors.forename: Ivo C J H
authors.initials: IC
authors.lastname: Post
authors.forename: Thomas M
authors.initials: TM
authors.lastname: van Gulik
authors.forename: Didier
authors.initials: D
authors.lastname: Payen
authors.forename: Can
authors.initials: C
authors.lastname: Ince
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007672:Kidney Cortex / Q000098:blood supply*; D007834:Lasers; D008297:Male; D055806:Microvessels / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013997:Time Factors