title: Simultaneous dual-tracer PET imaging of the rat brain and its application in the study of cerebral ischemia.
abstract: OBJECTIVE
  This study evaluates the performance of simultaneous dual-tracer technique (SDTT) in static positron emission tomography (PET) studies using 2-deoxy-2-[¹⁸F]fluoro-D-glucose and [¹³N]ammonium as radiotracers.
  
  
  METHODS
  The effects of applying SDTT either to the reconstructed image or directly to the sinogram, different rebinning algorithms, total acquisition time, and frame duration were investigated; first, using a specific phantom and later using an in vivo application of the study of cerebral ischemia.
  
  
  RESULTS
  The best results were obtained using the image method with single-slice rebinning and a total acquisition time of at least 20 min. Frame duration did not affect SDTT performance. The method was also applied in rats with transient cerebral ischemia to simultaneously study cerebral blood flow and cerebral glucose metabolism.
  
  
  CONCLUSIONS
  The results encourage the use of SDTT as it has very good potential for examining two different biological processes at the same time utilising rodent PET scanners.
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
authors.forename: Francisca P
authors.initials: FP
authors.lastname: Figueiras
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Jiménez
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
authors.forename: Deborah
authors.initials: D
authors.lastname: Pareto
authors.affiliation: CIC-BiomaGUNE, Unidad de Imagen, San Sebastián, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Gómez
authors.affiliation: CIC-BiomaGUNE, Unidad de Imagen, San Sebastián, Spain.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Llop
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
authors.forename: Raul
authors.initials: R
authors.lastname: Herance
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Rojas
authors.affiliation: Institut d'Alta Tecnologia-Parc de Recerca Biomèdica de Barcelona (PRBB), CRC Corporació Sanitària, Edifici PRBB c/Dr. Aiguader, 88, 08003, Barcelona, Spain. jgispert@crccorp.es.|CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain. jgispert@crccorp.es.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Gispert
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000473:pathology*; D002545:Brain Ischemia / Q000000981:diagnostic imaging*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D008297:Male; D019047:Phantoms, Imaging; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18