Lectin biosensing using digital analysis of Ru(II)-glycodendrimers.
A novel, digital, single-operation analytical method to study glycodendrimer-lectin interactions is described. Robust, highly fluorescent derivatives of tris(bipyridine)ruthenieum(II) ([Ru(bipy)(3)](2+)) bearing 2, 4, 6, or 18 mannose or galactose units were designed to perform molecular logic operations. Inputs for these systems were pH, N,N'-4,4'-bis(benzyl-2-boronic acid)bipyridinium dibromide, and different lectins (concanavalin A, Galantus nivalis agglutinin, and asialoglycoprotein). The relative change in fluorescence quantum yield of the Ru(II)-glycodendrimers served as output. Together, the fluorescent emission readout, the logic analysis of the photoinduced electron transfer, and the optical behavior provide a single-step method to quickly screen a glycodendrimer library and select the best dendrimer model for studying carbohydrate-lectin interactions.
Kikkeri
Raghavendra
R
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany.
Grünstein
Dan
D
Seeberger
Peter H
PH
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001212:Asialoglycoproteins / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000378:metabolism; D056831:Coordination Complexes; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D006601:Hexoses / Q000737:chemistry / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D001212:Asialoglycoproteins; D003208:Concanavalin A; D056831:Coordination Complexes; D050091:Dendrimers; D005456:Fluorescent Dyes; D005690:Galactose; D006601:Hexoses; D037102:Lectins; D008358:Mannose; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C063117:snowdrop lectin; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds