title: Altered Fos immunoreactivity in the hypothalamus after glucose administration in pre- and post-weaning malnourished rats.
abstract: The present study explored the effects of malnutrition and nutritional rehabilitation on the response to glucose in hypothalamic nuclei involved in metabolic homeostasis. Male Wistar rats were malnourished during gestation-lactation (MGL) or at weaning to 55 days (MPW). Two groups of rats were rehabilitated with a balanced diet until 90 days (MGL-R and MPW-R, respectively). After a glucose tolerance test (GTT), brains were processed for Fos immunoreactivity (Fos-IR). Both malnourished groups displayed hyperglycemia after GTT. MGL exhibited an increased number of Fos-IR neurons in the ventromedial hypothalamic nucleus (VMH), while MPW showed increased Fos-IR in the arcuate nucleus (ARC) and VMH and a decrease in the paraventricular nucleus (PVN), as compared with their controls. Nutritional rehabilitation normalized values of glucose after GTT in both groups, while low number of Fos-IR neurons remained in the ARC, PVN and VMH of MPW-R rats, indicating a deleterious, long-lasting effect after post-weaning malnutrition.
authors.affiliation: Departamento de Anatomía, Facultad de Medicina, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Miñana-Solis
authors.forename: Manuel
authors.initials: M
authors.lastname: Angeles-Castellanos
authors.forename: Ruud M
authors.initials: RM
authors.lastname: Buijs
authors.forename: Carolina
authors.initials: C
authors.lastname: Escobar
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000166:cytology; D001786:Blood Glucose / Q000032:analysis; D002452:Cell Count; D018753:Diet, Protein-Restricted; D005260:Female; D005947:Glucose / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008297:Male; D044342:Malnutrition / Q000178:diet therapy / Q000378:metabolism* / Q000534:rehabilitation; D008431:Maternal-Fetal Exchange; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology; D011247:Pregnancy; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology; D014886:Weaning
chemical_list: D001786:Blood Glucose; D016760:Proto-Oncogene Proteins c-fos; D005947:Glucose