Comparison of the sensitivity of three toxicity tests determining survival, inhibition of growth and colony-forming ability in human fibroblasts after incubation with environmental chemicals.
The sensitivity of three methods measuring basal cytotoxicity in human fibroblasts was compared. Cell survival, inhibition of growth and colony-forming ability were measured after 1 hr of incubation with nine environmental chemicals from different substance classes. The sensitivity of the method of determining inhibition of growth was similar to that of the test of colony-forming ability and was double that of a commonly used method to determine cell survival. Linear regression analysis showed that the three methods correlated well with each other, the strongest correlation being between colony-forming ability and growth.
Witte
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Carl von Ossietzky Universität Oldenburg, FB 7 und ICBM, Postfach 2503, D-26111 Oldenburg, Germany.
Frahmann
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Jacobi
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