title: Loxoscelism and negative pressure wound therapy (vacuum-assisted closure): an experimental study.
abstract: Brown recluse spider (Loxosceles) bites cause lesions ranging from chronic necrotic ulcers to acute life-threatening sepsis. Based on our experience in treating acute and chronic wounds with negative pressure, we postulated that vacuum-assisted closure (VAC) would be valuable in this application. Chester pigs were procured and injected with purified brown recluse spider venom, 1 µl of venom in two anterior sites and 0·1 µl of venom in two posterior sites on their dorsum. For each concentration of venom, treatment consisted of either VAC or dry, non adherent dressings (control group). Each day, the wounds were inspected and measured. For wounds receiving 1·0 µl of venom, the control wounds decreased in surface area to 50% of initial size after 7 days and none had healed, whereas VAC-treated wounds were less than 50% after 48 hours and completely healed and reepithelialised after 8 days. Wounds with 0·1 µl of venom had 50% reduction after 5 days with no complete healing for control wounds, and the VAC wounds were 50% after 48 hours and all had closed and reepithelialised after 5 days. Our experimental study showed an accelerated healing time in the animals treated with the VAC as compared with controls.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1075, USA.
authors.forename: S Lindsey
authors.initials: SL
authors.lastname: Wong
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Schneider
authors.forename: Louis C
authors.initials: LC
authors.lastname: Argenta
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Morykwas
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D009336:Necrosis; D054843:Negative-Pressure Wound Therapy / Q000379:methods*; D009779:Occlusive Dressings; D010727:Phosphoric Diester Hydrolases / Q000009:adverse effects*; D010865:Pilot Projects; D017592:Skin Care / Q000379:methods*; D001098:Spider Bites / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D013111:Spider Venoms / Q000009:adverse effects*; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases