Measurement of anti-beta amyloid antibodies in human blood.
The human IgG repertoire contains endogenous antibodies against beta amyloid (Aβ) that may be relevant to the pathogenesis and treatment of Alzheimer's disease. There have been widely disparate estimates of the levels of these antibodies in human plasma. We identify factors that have contributed to these disparities and describe improved methods for measuring anti-Aβ antibodies in blood. These methods include isolating immunoglobulin by thiophilic chromatography and using chaotropic salts to dislodge weakly bound antibodies without significantly reducing the binding of specific anti-Aβ antibodies. Using these methods, we show that human blood contains polyvalent IgG antibodies that bind to Aβ with relatively low avidity and specificity, as well as IgG antibodies that bind to linear and conformational epitopes on amyloid monomers and aggregates with moderate to high avidity.
Szabo
Paul
P
Department of Neurology and Neurosciences, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.
Mujalli
Diana M
DM
Rotondi
Matthew L
ML
Sharma
Rajal
R
Weber
Alfred
A
Schwarz
Hans-Peter
HP
Weksler
Marc E
ME
Relkin
Norman
N
D000544:Alzheimer Disease / Q000097:blood / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000097:blood* / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood*; D011485:Protein Binding / Q000276:immunology
D016229:Amyloid beta-Peptides; D007074:Immunoglobulin G