title: Dendrobium candidum extract increases the expression of aquaporin-5 in labial glands from patients with Sjögren's syndrome.
abstract: This study aimed to investigate the mechanism of Dendrobium candidum extract in promoting expression of aquaporin-5 for treatment of Sjögren's syndrome (SS). Sixteen patients with SS suffered from deficient secretion of saliva due to an autoimmune destruction of salivary glands leading to dry mouth symptoms (xerostomia). However, glandular dysfunction also occurred without destruction. Based upon its abnormal distribution in SS salivary glands, a potential role of the water channel protein aquaporin-5 (AQP-5) in the pathogenesis of SS was proposed. After oral administration of D. candidum extracted liquid (DCEL) for 1 week, saliva and salivary gland biopsies from labial glands of patients were collected and examined by employing immunoreactivity and immunohistochemistry techniques. Results showed that salivary secretion increased by about 65% in patients treated with DCEL as compared with the control group. Higher labeling indices (percentage of acinus area immunoreactive for AQP-5) in the biopsies were found in SS patients who had taken DCEL. This study demonstrated that D. candidum would regulate the expression of AQP-5 in labial glands of SS patients and thereby promoted secretion of saliva to improve dry mouth symptoms.
authors.affiliation: Department of Oral & Maxillofacial Surgery, Central Hospital of Fuling, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Yi-Bin
authors.initials: YB
authors.lastname: Feng
authors.forename: Tong
authors.initials: T
authors.lastname: Yao
authors.forename: Jack Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Ren-Min
authors.initials: RM
authors.lastname: Yao
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Fei-Zhi
authors.initials: FZ
authors.lastname: Mo
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiao
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
authors.forename: Ze-Min
authors.initials: ZM
authors.lastname: Li
authors.forename: Stephen Cho Wing
authors.initials: SC
authors.lastname: Sze
authors.forename: Kalin Yanbo
authors.initials: KY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism*; D001706:Biopsy; D031667:Dendrobium; D005260:Female; D006801:Humans; D008046:Lip; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012463:Saliva / Q000378:metabolism*; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D051402:Aquaporin 5; D010936:Plant Extracts