Peering into biological nanopore: a practical technology to single-molecule analysis.
As a unique technique at the singe-molecule level to explore the distribution and temporal order of events, nanopore technology has attracted increasing attention. In comparison to the previous applications in DNA sequencing, this Focus Review highlights the technical details of biological nanopores, especially alpha-hemolysin, in the analysis of peptides and proteins. The instrument configurations, experimental interferences, and data analysis including the conformation of peptides and proteins and their interactions for single-molecule detection are discussed.
Wang
Hai-Yan
HY
Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, 130 Meilong Road Shanghai 200237, China.
Ying
Yi-Lun
YL
Li
Yang
Y
Long
Yi-Tao
YT
D015374:Biosensing Techniques; D006460:Hemolysin Proteins / Q000737:chemistry*; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D006460:Hemolysin Proteins; D008024:Ligands; D008051:Lipid Bilayers