Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity.
The aerial parts of selected Potentilla species (P. anserina, P. argentea, P. erecta, P. fruticosa, P. grandiflora, P. nepalensis, P. norvegica, P. pensylvanica, P. crantzii and P. thuringiaca) were investigated in order to determine their contents of polyphenolic compounds. The results showed that P. fruticosa has relatively high concentrations of tannins (167.3 +/- 2.0 mg/g dw), proanthocyanidins (4.6 +/- 0.2 mg/g dw) and phenolic acids (16.4 +/- 0.8 mg/g dw), as well as flavonoids (7.0 +/- 1.1 mg/g dw), calculated as quercetin. Furthermore, we investigated the in vitro inhibitory effects of aqueous extracts from these species against cariogenic Streptococcus spp. strains. It was found that the tested samples moderately inhibit the growth of oral streptococci. However, all the preparations exhibited inhibitory effects on water-insoluble alpha-(1-->3)-, alpha-(1-->6)-linked glucan (mutan) and artificial dental plaque formation. The extract from P. fruticosa showed the highest anti-biofilm activities, with minimum mutan and biofilm inhibition concentrations of 6.25-25 and 50-100 microg/mL, respectively. The results indicate that the studied Potentilla species could be a potential plant material for extracting biologically active compounds, and could become a useful supplement for pharmaceutical products as a new anticariogenic agent in a wide range of oral care products.
Tomczyk
Michał
M
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland. tomczyk@umwb.edu.pl
Pleszczyńska
Małgorzata
M
Wiater
Adrian
A
D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D003729:Dental Care; D003773:Dental Plaque / Q000188:drug therapy*; D005419:Flavonoids / Q000032:analysis*; D009910:Oral Hygiene; D010636:Phenols / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry; D059808:Polyphenols; D031987:Potentilla / Q000737:chemistry*; D013291:Streptococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols