title: Redundant and unique roles of coronin proteins in Dictyostelium.
abstract: Dictyostelium discoideum harbors a short (CRN12) and a long coronin (CRN7) composed of one and two beta-propellers, respectively. They are primarily present in the cell cortex and cells lacking CRN12 (corA⁻) or CRN7 (corB⁻) have defects in actin driven processes. We compared the characteristics of a mutant cell line (corA⁻/corB⁻) lacking CRN12 and CRN7 with the single mutants focusing on cytokinesis, phagocytosis, chemotaxis and development. Cytokinesis, uptake of small particles, and developmental defects were not enhanced in the corA⁻/corB⁻ strain as compared to the single mutants, whereas motility and phagocytosis of yeast particles were more severely impaired. It appears that although both proteins affect the same processes they do not act in a redundant manner. Rather, they often act antagonistically, which is in accordance with their proposed roles in the actin cytoskeleton where CRN12 acts in actin disassembly whereas CRN7 stabilizes actin filaments and protects them from disassembly.
authors.affiliation: Institute for Biochemistry I, Center for Molecular Medicine Cologne, University of Cologne, Germany.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Shina
authors.forename: Annette
authors.initials: A
authors.lastname: Müller-Taubenberger
authors.forename: Can
authors.initials: C
authors.lastname: Unal
authors.forename: Michael
authors.initials: M
authors.lastname: Schleicher
authors.forename: Michael
authors.initials: M
authors.lastname: Steinert
authors.forename: Ludwig
authors.initials: L
authors.lastname: Eichinger
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Blau-Wasser
authors.forename: Gernot
authors.initials: G
authors.lastname: Glöckner
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002633:Chemotaxis / Q000502:physiology; D048749:Cytokinesis / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli; D017353:Gene Deletion; D016952:Legionella pneumophila; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D014162:Transfection
chemical_list: D000199:Actins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C072061:coronin proteins