Nuclear and cytoplasmic expression of Met in oral squamous cell carcinoma and in an organotypic oral cancer model.
Met, the hepatocyte growth factor receptor, is important in transducing signals for tumour growth and metastasis. The aim of this study was to examine the pattern of Met expression and its value as a prognostic factor in oral squamous cell carcinomas (OSCCs). The material consisted of 53 OSCCs and five healthy controls from normal oral mucosa supplied with cell lines, 10 organotypic models supplied with oral cancer cells, and three organotypic models supplied with normal keratinocytes. Met protein expression was assessed by immunohistochemistry and western blotting. Met expression was scarce and limited to the basal layer in normal oral mucosa, but was more extensive in the tumours. Cytoplasmic expression of Met was found in the majority of the tumours, and nuclear expression was found in 72%, including a high fraction of the cells located at the invasive front. Organotypic models with normal or malignant oral cells yielded principally similar results as in the mucosa and the cancers, respectively. A smaller amount of Met immunoreactivity was detected, by western blotting, in the nuclear fraction of cultured oral cancer cells. In conclusion, Met was upregulated in OSCCs and was also found in the nucleus. However, Met was not a marker for prognosis in this study.
Brusevold
Ingvild J
IJ
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway. ingviljb@odont.uio.no
Søland
Tine M
TM
Khuu
Cuong
C
Christoffersen
Thoralf
T
Bryne
Magne
M
D000230:Adenocarcinoma / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D001943:Breast Neoplasms / Q000473:pathology; D002277:Carcinoma / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018929:Cell Culture Techniques; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003593:Cytoplasm / Q000648:ultrastructure*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology; D009062:Mouth Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D011379:Prognosis; D019859:Proto-Oncogene Proteins c-met / Q000032:analysis*; D012878:Skin Neoplasms / Q000473:pathology; D054457:Tissue Scaffolds; D014062:Tongue Neoplasms / Q000473:pathology; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D007633:Keratins; D019859:Proto-Oncogene Proteins c-met