Efficient industrial dye decolorization by Bacillus sp. VUS with its enzyme system.
This work presents role of different enzymes in decolorization of industrial dye Orange T4LL by Bacillus sp. VUS. Bacillus sp. strain VUS decolorized dye Orange T4LL, under static anoxic condition in 24 h. During decolorization of Orange T4LL a significant induction in the activities of lignin peroxidase, tyrosinase, and reductases (NADH-DCIP, azo, and riboflavin) was observed. The biodegradation was monitored by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, and high performance liquid chromatography. The final products 4-methyl-2-o-tolylazo-benzene-1,3-diamine and [3-(phenyl-hydrazono)-cyclohexa-1,4-dienyl]-methanol were characterized by gas chromatography-mass spectroscopy. Phytotoxicity, COD, and BOD revealed non-toxicity of degraded products. Phytotoxicity study demonstrated non-toxicity of the biodegraded products for crop plants with respect to Triticum aestivum and Sorghum bicolor. Bacillus sp. VUS with its enzyme system could be a useful tool for textile effluent treatment.
Dawkar
Vishal V
VV
Department of Biochemistry, Shivaji University, Kolhapur 416004, India. vvdawkar@gmail.com
Jadhav
Umesh U
UU
Tamboli
Dhawal P
DP
Govindwar
Sanjay P
SP
D000704:Analysis of Variance; D001407:Bacillus / Q000201:enzymology*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D010544:Peroxidases / Q000378:metabolism; D011808:Quinone Reductases / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D004396:Coloring Agents; D010544:Peroxidases; C042858:lignin peroxidase; D014442:Monophenol Monooxygenase; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; C033633:dichlorophenolindophenol reductase; D009601:Nitroreductases; C021106:azoreductase