title: The alcohol dehydrogenase system in the xylose-fermenting yeast Candida maltosa.
abstract: BACKGROUND
  The alcohol dehydrogenase (ADH) system plays a critical role in sugar metabolism involving in not only ethanol formation and consumption but also the general "cofactor balance" mechanism. Candida maltosa is able to ferment glucose as well as xylose to produce a significant amount of ethanol. Here we report the ADH system in C. maltosa composed of three microbial group I ADH genes (CmADH1, CmADH2A and CmADH2B), mainly focusing on its metabolic regulation and physiological function.
  
  
  RESULTS
  Genetic analysis indicated that CmADH2A and CmADH2B tandemly located on the chromosome could be derived from tandem gene duplication. In vitro characterization of enzymatic properties revealed that all the three CmADHs had broad substrate specificities. Homo- and heterotetramers of CmADH1 and CmADH2A were demonstrated by zymogram analysis, and their expression profiles and physiological functions were different with respect to carbon sources and growth phases. Fermentation studies of ADH2A-deficient mutant showed that CmADH2A was directly related to NAD regeneration during xylose metabolism since CmADH2A deficiency resulted in a significant accumulation of glycerol.
  
  
  CONCLUSIONS
  Our results revealed that CmADH1 was responsible for ethanol formation during glucose metabolism, whereas CmADH2A was glucose-repressed and functioned to convert the accumulated ethanol to acetaldehyde. To our knowledge, this is the first demonstration of function separation and glucose repression of ADH genes in xylose-fermenting yeasts. On the other hand, CmADH1 and CmADH2A were both involved in ethanol formation with NAD regeneration to maintain NADH/NAD ratio in favor of producing xylitol from xylose. In contrast, CmADH2B was expressed at a much lower level than the other two CmADH genes, and its function is to be further confirmed.
authors.affiliation: Centre of Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Lin
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Wang
authors.forename: Dajun
authors.initials: D
authors.lastname: Lu
authors.forename: Zilong
authors.initials: Z
authors.lastname: Li
authors.forename: Changsheng
authors.initials: C
authors.lastname: Wu
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D005285:Fermentation / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014994:Xylose; D000426:Alcohol Dehydrogenase