title: Characterization of canine platelet adhesion to extracellular matrix proteins.
abstract: Canine platelets have been extensively studied but little is known about specific aspects such as adhesion. Platelet adhesion is a critical step during haemostasis and thrombosis as well as during inflammatory and immunopathogenic responses. The aim of this study was to evaluate the adhesive properties of canine platelets using fibrinogen and collagen as substrates immobilized on plates. Adhesion was monitored for 120 min and the effect of adenosine 5'-diphosphate (ADP) was assayed. The results showed that canine platelets displayed good adhesion activity that was significantly time-dependent. Moreover, ADP was able to enhance platelet adhesion in a dose-dependent manner. The findings aid knowledge of the adhesion process and suggest a specific role of surface platelet receptors in mediating the interaction with extracellular matrix proteins.
authors.affiliation: Department of Biological Structures, Functions and Technologies, Faculty of Veterinary Medicine, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pelagalli
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Pero
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mastellone
authors.forename: Anna
authors.initials: A
authors.lastname: Cestaro
authors.forename: Simona
authors.initials: S
authors.lastname: Signoriello
authors.forename: Pietro
authors.initials: P
authors.lastname: Lombardi
authors.forename: Luigi
authors.initials: L
authors.lastname: Avallone
mesh_terms: D000244:Adenosine Diphosphate; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D003094:Collagen; D004285:Dogs; D016326:Extracellular Matrix Proteins; D005340:Fibrinogen; D008297:Male; D010973:Platelet Adhesiveness; D013997:Time Factors
chemical_list: D016326:Extracellular Matrix Proteins; D000244:Adenosine Diphosphate; D005340:Fibrinogen; D003094:Collagen