The study of some haematological and serum biochemical parameters of juvenile beluga (Huso huso) fed oligofructose.
A study was conducted to investigate the effects of dietary oligofructose (1, 2 and 3%) on the blood profiles of beluga (Huso huso) juveniles (18.77 ± 0.76 g) compared to fish fed an un-supplemented diet. After 7 weeks of feeding on the experimental diets, haematological parameters, metabolic products (cholesterol, glucose and total protein) and serum enzymes (lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase) were measured. Compared to the control group (0% oligofructose), dietary oligofructose had no effect on red blood cell counts (RBC), mean corpuscular volume (MCV), mean cellular haemoglobin (MCH) or mean cell haemoglobin concentration (MCHC) (P > 0.05). However, haemoglobin (Hb) concentration, leucocyte (WBC) levels and the proportion of lymphocytes were significantly higher (P > 0.05) in the 2% oligofructose fed fish than in the 3% oligofructose fed fish. Additionally, haematocrit (Hct) values (P = 0.049) and the proportion of lymphocytes (P ≤ 0.01) were significantly higher in the 2% oligofructose group than in the control group. Although serum glucose and total protein remained unaffected, serum cholesterol was significantly lower in the 2% oligofructose group than in the control and 3% oligofructose group (P < 0.05). The results of the present study showed that oligofructose had no significant effects on serum lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase. These results indicate that fish blood profiles could be affected by prebiotics, which should be taken into account in future studies.
Hoseinifar
Seyed Hossein
SH
Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. hoseinifar@ut.ac.ir
Mirvaghefi
Alireza
A
Merrifield
Daniel L
DL
Amiri
Bagher Mojazi
BM
Yelghi
Saeed
S
Bastami
Kazem Darvish
KD
D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D004032:Diet / Q000662:veterinary; D005399:Fishes / Q000097:blood* / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D056692:Prebiotics; D044967:Serum / Q000737:chemistry / Q000166:cytology / Q000201:enzymology
D009844:Oligosaccharides; D056692:Prebiotics; C120489:oligofructose