Pancreatic acinar cells: molecular insight from studies of signal-transduction using transgenic animals.
Pancreatic acinar cells are classical exocrine gland cells. The apical regions of clusters of coupled acinar cells collectively form a lumen which constitutes the blind end of a tube created by ductal cells - a structure reminiscent of a "bunch of grapes". When activated by neural or hormonal secretagogues, pancreatic acinar cells are stimulated to secrete a variety of proteins. These proteins are predominately inactive digestive enzyme precursors called "zymogens". Acinar cell secretion is absolutely dependent on secretagogue-induced increases in intracellular free Ca(2+). The increase in [Ca(2+)](i) has precise temporal and spatial characteristics as a result of the exquisite regulation of the proteins responsible for Ca(2+) release, Ca(2+) influx and Ca(2+) clearance in the acinar cell. This brief review discusses recent studies in which transgenic animal models have been utilized to define in molecular detail the components of the Ca(2+) signaling machinery which contribute to these characteristics.
Yule
David I
DI
Department of Pharmacology and Physiology, University of Rochester Medical School, Rochester, NY 14642, USA. David Yule@urmc.rochester.edu
D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism; D005088:Exocrine Glands / Q000166:cytology* / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
D002118:Calcium