title: Relationship of oxidative stress and endothelial dysfunction in sleep apnoea.
abstract: The aim of the present study was to evaluate ischaemic reactive hyperaemia (IRH) in obstructive sleep apnoea (OSA) and its relationship with oxidative stress. We studied 69 consecutive patients referred to our Sleep Unit (Reina Sofia University Hospital, Cordoba, Spain). Patients with chronic diseases or those taking medication were excluded. IRH was assessed before and after polysomnography. Morning IRH and oxidative stress markers were compared between patients with (apnoea-hypopnoea index (AHI) ≥ 5) and without (AHI < 5) OSA. Measurements were repeated in 25 severe OSA patients after continuous positive airway pressure (CPAP) therapy. We included 46 OSA patients (mean ± sd AHI 49 ± 32.1) and 23 non-OSA subjects (AHI 3 ± 0.9). The OSA patients showed a significant worsening of morning IRH, and a significant increase in malondialdehyde and 8-hydroxydeoxyguanosine levels. Only the oxygen desaturation index independently explained morning IRH, while malondialdehyde levels showed a weak effect on IRH. In severe OSA patients, IRH improved significantly after CPAP treatment, as did malondialdehyde, 8-hydroxydeoxyguanosine and protein carbonyl levels. In OSA patients, endothelial dysfunction and oxidative stress were observed, and IRH worsened after sleep. The increase in oxidative stress was not associated with IRH, while intermittent hypoxia was strongly associated with IRH. In severe OSA patients, CPAP treatment improved oxidative stress and endothelial function.
authors.affiliation: Sleep Unit, Dept of Respiratory Medicine, Reina Sofia University Hospital, University Hospital/University of Cordoba, CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Madrid, Spain. bjg01co@hotmail.com
authors.forename: B
authors.initials: B
authors.lastname: Jurado-Gámez
authors.forename: M C
authors.initials: MC
authors.lastname: Fernandez-Marin
authors.forename: J L
authors.initials: JL
authors.lastname: Gómez-Chaparro
authors.forename: L
authors.initials: L
authors.lastname: Muñoz-Cabrera
authors.forename: J
authors.initials: J
authors.lastname: Lopez-Barea
authors.forename: F
authors.initials: F
authors.lastname: Perez-Jimenez
authors.forename: J
authors.initials: J
authors.lastname: Lopez-Miranda
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D017286:Polysomnography / Q000379:methods; D011446:Prospective Studies; D020181:Sleep Apnea, Obstructive / Q000378:metabolism*
chemical_list: D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D010100:Oxygen