title: STOX2 but not STOX1 is differentially expressed in decidua from pre-eclamptic women: data from the Second Nord-Trondelag Health Study.
abstract: Variation in the Storkhead box-1 (STOX1) gene has previously been associated with pre-eclampsia. In this study, we assess candidate single nucleotide polymorphisms (SNPs) in STOX1 in an independent population cohort of pre-eclamptic (n = 1.139) and non-pre-eclamptic (n = 2.269) women (the HUNT2 study). We also compare gene expression levels of STOX1 and its paralogue, Storkhead box-2 (STOX2) in decidual tissue from pregnancies complicated by pre-eclampsia and/or fetal growth restriction (FGR) (n = 40) to expression levels in decidual tissue from uncomplicated pregnancies (n = 59). We cannot confirm association of the candidate SNPs to pre-eclampsia (P > 0.05). For STOX1, no differential gene expression was observed in any of the case groups, whereas STOX2 showed significantly lower expression in deciduas from pregnancies complicated by both pre-eclampsia and FGR as compared with controls (P = 0.01). We further report a strong correlation between transcriptional alterations reported previously in choriocarcinoma cells over expressing STOX1A and alterations observed in decidual tissue of pre-eclamptic women with FGR.
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim 7006, Norway. mona.n.fenstad@ntnu.no
authors.forename: M H
authors.initials: MH
authors.lastname: Fenstad
authors.forename: M P
authors.initials: MP
authors.lastname: Johnson
authors.forename: M
authors.initials: M
authors.lastname: Løset
authors.forename: S B
authors.initials: SB
authors.lastname: Mundal
authors.forename: L T
authors.initials: LT
authors.lastname: Roten
authors.forename: I P
authors.initials: IP
authors.lastname: Eide
authors.forename: L
authors.initials: L
authors.lastname: Bjørge
authors.forename: R K
authors.initials: RK
authors.lastname: Sande
authors.forename: A K
authors.initials: AK
authors.lastname: Johansson
authors.forename: T D
authors.initials: TD
authors.lastname: Dyer
authors.forename: S
authors.initials: S
authors.lastname: Forsmo
authors.forename: J
authors.initials: J
authors.lastname: Blangero
authors.forename: E K
authors.initials: EK
authors.lastname: Moses
authors.forename: R
authors.initials: R
authors.lastname: Austgulen
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015331:Cohort Studies; D003656:Decidua / Q000378:metabolism*; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D002352:Carrier Proteins; C500784:STOX1 protein, human