title: Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages.
abstract: Macrophage colony-stimulating factor (M-CSF) (also known as CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have distinct effects on macrophage lineage populations, which are likely to be contributing to their functional heterogeneity. A comparative proteomic analysis of proteins released into culture media from such populations after M-CSF and GM-CSF exposure was carried out. Adherent macrophage populations, termed bone marrow-derived macrophage (BMM) and GM-BMM, were generated after treatment of murine bone marrow precursors with M-CSF and GM-CSF, respectively. Proteins in 16-h serum-free conditioned media (CM) were identified by two-dimensional gel electrophoresis and mass spectrometry. Respective protein profiles from BMM and GM-BMM CM were distinct and there was the suggestion of a switch from primarily signal peptide-driven secretion to non-classical secretion pathways from BMM to GM-BMM. Extracellular expression of cathepsins (lysosomal proteases) and their inhibitors seems to be a characteristic difference between these macrophage cell types with higher levels usually observed in BMM-CM. Furthermore, we have identified a number of proteins in BMM-CM and GM-BMM-CM that could be involved in various tissue regeneration and inflammatory (immune) processes, respectively. The uncharacterized protein C19orf10, a protein found at high levels in the synovial fluid of arthritis patients, was also differentially regulated; its extracellular levels were upregulated in the presence of GM-CSF.
authors.affiliation: Department of Medicine, CRC for Chronic Inflammatory Diseases, The University of Melbourne, The Royal Melbourne Hospital, Parkville, Victoria, Australia. m.bailey@uq.edu.au
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Bailey
authors.forename: Derek C
authors.initials: DC
authors.lastname: Lacey
authors.forename: Bernard V A
authors.initials: BV
authors.lastname: de Kok
authors.forename: Paul D
authors.initials: PD
authors.lastname: Veith
authors.forename: Eric C
authors.initials: EC
authors.lastname: Reynolds
authors.forename: John A
authors.initials: JA
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019295:Computational Biology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D021381:Protein Transport / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D007378:Interleukins; D020543:Proteome; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor