Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid.
A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid.
Gallardo
Oscar
O
Department of Microbiology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain.
Fernández-Fernández
María
M
Valls
Cristina
C
Valenzuela
Susana Valeria
SV
Roncero
M Blanca
MB
Vidal
Teresa
T
Díaz
Pilar
P
Pastor
F I Javier
FI
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D057886:Bleaching Agents / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D005052:Eucalyptus / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism
D057886:Bleaching Agents; D009844:Oligosaccharides; C076151:Kraft lignin; D008031:Lignin; D014995:Xylosidases