title: Anti-complement activity of isolated compounds from the roots of Clerodendrum bungei Steud.
abstract: To determine the anti-complement activity of natural diterpenes, chromatographic separation of the acetone-soluble fraction from the roots of Clerodendrum bungei (Verbenaceae) led to the isolation of five diterpenoids. An acetone-soluble extract of the roots of C. bungei exhibited significant anti-complement activity on the classical pathway complement system, which was expressed as total hemolytic activity. Five compounds isolated from the roots of C. bungei, namely 12-O-β-d-glucopyranosyl-3,11,16-trihydroxyabieta-8,11,13-triene (1), 3,12-O-β-d-diglucopyranosyl-11,16-dihydroxyabieta-8,11,13-triene (2), ajugaside A (3), uncinatone (4) and 19-hydroxyteuvincenone F (5). Compounds 1, 2, 3, 4 and 5 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 24 µm, 138 µm, 116 µm, 87 µm and 232 µm. Among the compounds tested, 1 showed the most potent anti-complement activity (IC(50), 24 µm).
authors.affiliation: Department of Animal Science and Environment, Konkuk University, Seoul, 143-307, South Korea.
authors.forename: Soo-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Sang-Buem
authors.initials: SB
authors.lastname: Cho
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Moon
mesh_terms: D032409:Clerodendrum / Q000737:chemistry*; D003171:Complement Pathway, Classical / Q000187:drug effects*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D004224:Diterpenes; D010936:Plant Extracts