Endocrine controls of primary adult human stem cell biology: thyroid hormones stimulate keratin 15 expression, apoptosis, and differentiation in human hair follicle epithelial stem cells in situ and in vitro.
Here we demonstrate that physiological concentrations of the thyroid hormones T3 and T4 enhance the KERATIN 15 promoter activity and expression in epithelial stem cells of adult human scalp hair follicles in situ and in vitro. Additionally, T3 and T4 stimulate expression of the immuno-inhibitory surface molecule CD200. Subsequently, T3 and T4 induce apoptosis and differentiation and inhibit clonal growth of these progenitor cells in vitro. These data suggest that human hair follicle bulge-derived epithelial stem cells underlie profound, previously unknown hormonal regulation by thyroid hormones, and show that primary human keratin 15-GFP+ progenitor cells can be exploited to further elucidate fundamental endocrine controls of human epithelial stem cells.
Tiede
S
S
Department of Dermatology, University of Lübeck, Lübeck, Germany. stephan.tiede@uk-sh.de
Bohm
K
K
Meier
N
N
Funk
W
W
Paus
R
R
D000328:Adult; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D053569:Keratin-15 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009924:Organ Culture Techniques; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013974:Thyroxine / Q000494:pharmacology / Q000502:physiology; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*
C116015:KRT15 protein, human; D053569:Keratin-15; D014284:Triiodothyronine; D013974:Thyroxine