Detection of DNA hybridizations using solid-state nanopores.
We report an experimental study of using DNA translocation through solid-state nanopores to detect the sequential arrangement of two double-stranded 12-mer hybridization segments on a single-stranded DNA molecule. The sample DNA is a trimer molecule formed by hybridizing three single-stranded oligonucleotides. A polystyrene bead is attached to the end of the trimer DNA, providing a mechanism in slowing down the translocation and suppressing the thermal diffusion, thereby allowing the detection of short features of DNA by standard patch-clamp electronics. The electrical signature of the translocation of a trimer molecule through a nanopore has been identified successfully in the temporal traces of ionic current. The results reported here represent the first successful attempt in using a solid-state nanopore as an ionic scanning device in resolving individual hybridization segments (or 'probes') on a DNA molecule.
Balagurusamy
Venkat S K
VS
Department of Physics, Brown University, Providence, RI 02912, USA.
Weinger
Paul
P
Ling
Xinsheng Sean
XS
D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015723:Gene Library; D008957:Models, Genetic; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D016062:Porosity; D017422:Sequence Analysis, DNA / Q000379:methods*
D009841:Oligonucleotides; D004247:DNA