title: Establishment of an axenic culture of microcystin-producing Microcystis aeruginosa isolated from a Korean reservoir.
abstract: In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.
authors.affiliation: Department of Environmental Engineering, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Ah-Won
authors.initials: AW
authors.lastname: Han
authors.forename: Kyoung-Hee
authors.initials: KH
authors.lastname: Oh
authors.forename: Weon-Hwa
authors.initials: WH
authors.lastname: Jheong
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Cho
mesh_terms: D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007723:Korea; D052998:Microcystins / Q000235:genetics / Q000378:metabolism*; D046931:Microcystis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D052998:Microcystins; C078588:microcystin