title: A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of escin Ia and escin Ib in human plasma: application to a pharmacokinetic study after intravenous administration.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of escin Ia and escin Ib in human plasma. After a solid-phase extraction (SPE), the analytes were separated on a Zorbax Extend C(18) column by isocratic elution with a mobile phase of methanol-acetonitrile-10 mm ammonium acetate (27:27:46, v/v/v) at a flow rate of 1.0 mL/min and analyzed by mass spectrometry in the positive ion multiple reaction monitoring mode. The precursor to product ion transitions of m/z 1131.8 → 807.6 was used to quantify escin Ia and escin Ib. Good linearity was achieved over a wide range of 2.00-900 ng/mL for escin Ia and 1.50-662 ng/mL for escin Ib. The intra- and inter-day precisions (as relative standard deviation) were less than 11% for each QC level of escin Ia and escin Ib. The accuracies (as relative error) were within ±5.27% for escin Ia and within ±4.07% for escin Ib. The method was successfully employed in a pharmacokinetic study after a single intravenous infusion administration of sodium aescinate injection containing 10 mg escin to each of the 10 healthy volunteers.
authors.affiliation: Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130021, People's Republic of China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Liu
authors.forename: Xiujun
authors.initials: X
authors.lastname: Wu
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Wang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
authors.forename: Yimin
authors.initials: Y
authors.lastname: Cui
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004928:Escin / Q000008:administration & dosage / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C409970:escin Ib; D004928:Escin; C115101:escin Ia