Manzamine A as a novel inhibitor of herpes simplex virus type-1 replication in cultured corneal cells.
This study investigated the putative inhibitory effect of manzamine A on HSV-1 infection. Our results indicated that manzamine A effectively inhibited viral replication and infection in the cell line SIRC, a corneal cell line, at 1 µM. The existing anti-HSV-1 drug acyclovir was analyzed and showed a comparable activity at 50 µM. Plaque assays demonstrated that manzamine A reduced the release of infectious virus by 10 (11)-fold. RTPCR assays indicated that HSV-1 virion host shutoff (vhs) activity and ICP0 transcription were decreased by manzamine A treatment. These results bode well for the development of manzamines as potential leads to reduce viral infection in corneal cells and to prevent HSV-1-induced eye infections such as keratitis.
Palem
Jayavardhana R
JR
Department of Basic Pharmaceutical Sciences, College of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71201, USA.
Bedadala
Gautam R
GR
El Sayed
Khalid A
KA
Hsia
Shao-chung V
SC
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D003315:Cornea / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D011161:Porifera / Q000737:chemistry; D011817:Rabbits; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D002227:Carbazoles; C078290:manzamine A