Structural investigation of an antibacterial polysaccharide from Streptomyces virginia H03.
The chemical structure of a polysaccharide from the broth of cultured Streptomyces virginia H03 was investigated. There might be 1-->2 and 1-->4 and no 1-->6 glycosidic linkages in the polysaccharide according to periodate oxidation and Smith degradation. Four fragments including 2,3,4,6-Me4-Man, 2,3,6-Me3-Gal, 2,4,6-Me3-Glc, and 3,6-Me2-Man were found in the methylated polysaccharide. Furthermore, the polysaccharide has a beta-Glc(1-->4)-alpha-Man(1-->4)-alpha-Gal(1-->3)-linked backbone and a branch at the C-2 position of (1-->2)-linked mannose residues as determined by the method of nuclear magnetic resonance (NMR) spectroscopy. The assumed structure of the polysaccharide is [formula see text]. Additionally, the polysaccharide has a wide antibiogram and was found to be most effective against Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli.
He
Feng
F
Institute of Resource Biology & Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Yang
Ying
Y
Yang
Guang
G
Yu
Longjiang
L
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D001412:Bacillus subtilis / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D002236:Carbohydrate Conformation; D004926:Escherichia coli / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008958:Models, Molecular; D009561:Nisin / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013302:Streptomyces / Q000737:chemistry*; D020068:Zygosaccharomyces / Q000187:drug effects
D000900:Anti-Bacterial Agents; D011134:Polysaccharides; D009561:Nisin