Lack of prognostic value of topoisomerase II alpha in patients with breast cancer: analysis with tissue microarray.
BACKGROUND
The creation of a tissue microarray (TMA) allows for the rapid immunohistochemical analysis of thousands of tissue samples in a parallel fashion. This study applied TMA in order to analyse the topoisomerase II alpha status in breast cancer and to elucidate its relationship in breast cancer biology.


METHODS
Archived tissue specimens from 94 patients with primary invasive breast cancer were selected and the topoisomerase II alpha expression was analysed by TMA. Data concerning age, oestrogen receptor status, histological grading and TNM staging were also collected.


RESULTS
There were 20 patients (21.3%) with 1+ expression in topoisomerase II alpha, 33 patients (35.1%) with 2+ expression and 41 patients (43.6%) with 3+ expression. By multivariate analysis, oestrogen receptor status, histological grading and TNM staging were found to be significantly related to the overall five-year survival rate.


CONCLUSIONS
Topoisomerase II alpha expression failed to demonstrate prognostic value in patients with breast cancer.
Sheen-Chen
Shyr-Ming
SM
Department of Surgery, Chang Gung Memorial Hospital, Kaohsiung Medical Center, 123, Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien, Taiwan. smsheen@yahoo.com
Huang
Chun-Ying
CY
Zhang
Hao
H
Huang
Chao-Cheng
CC
Tang
Rei-Ping
RP
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D001943:Breast Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D004250:DNA Topoisomerases, Type II / Q000032:analysis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate; D046888:Tissue Array Analysis / Q000379:methods*
D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D004250:DNA Topoisomerases, Type II