title: The gene expression of the myocardial lipid droplet protein is highly regulated by PPARgamma in adipocytes differentiated from MEFs or SVCs.
abstract: We demonstrate that expression of the myocardial lipid droplet protein (MLDP) and ERalpha observed in adipose tissues is undetectable in 3T3-L1 cells but detectable in mouse embryonic fibroblasts (MEFs) and stromal-vascular cells (SVCs) during adipocyte differentiation. MLDP gene expression in MEFs or SVCs is induced by treatment with a PPARgamma agonist or forced expression of PPARgamma, indicating that PPARgamma enhances MLDP expression during adipogenesis. PCR analyses reveal the dual expression of SREBP-1a and SREBP-1c in MEFs and SVCs as well as white adipose tissues unlike the predominant expression of SREBP-1a in 3T3-L1 cells. These results suggest that MEFs and SVCs are useful model cells for examining function of MLDP in lipid droplet formation and adipocyte differentiation.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Shinoda
authors.forename: Jun
authors.initials: J
authors.lastname: Inoue
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Sato
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D004730:Endothelium, Vascular / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D047908:Intracellular Signaling Peptides and Proteins; C516105:OXPAT protein, mouse; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1