Cloning, expression, and polymorphisms of natural killer cell receptor NCR1 in pigs.
NCR1 (NKp46) is expressed on the surfaces of natural killer cells and recognizes hemagglutinin on the influenza virus. We cloned the NCR1 gene in pigs and found that porcine NCR1 was minimally expressed in the thymus, suggesting that NCR1 could be a useful marker of natural killer cells in pigs. We observed three nonsynonymous single nucleotide polymorphisms and one deletion of three nucleotides in the coding sequence of porcine NCR1; these may affect the function of NCR1. The polymorphisms detected here may be useful markers for breeding for influenza resistance in pigs.
Jozaki
Kosuke
K
Kagoshima University, Japan.
Shinkai
Hiroki
H
Morozumi
Takeya
T
Tanaka-Matsuda
Maiko
M
Eguchi-Ogawa
Tomoko
T
Wada
Yasuhiko
Y
Uenishi
Hirohide
H
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics* / Q000276:immunology; D013950:Thymus Gland / Q000378:metabolism / Q000502:physiology
D055627:Natural Cytotoxicity Triggering Receptor 1; D012313:RNA