Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system.
We have developed a filter-chip and optical detection system for rapid antibiotic efficacy screening. The filter-chip consisted of a 1-mL reservoir and an anodic aluminum oxide (AAO) nanoporous membrane. Sample solution with liquid growth media, bacteria, and antibiotics was incubated in the reservoir for a specific period of time. The number of live bacteria on the surface of membrane was counted after the incubation with antibiotics and filtration. Using this biosensing system, we have demonstrated a 1-h antibiotic screening for patients' clinical samples, significantly faster than the conventional antibiotic susceptibility tests that typically take more than 24h. This rapid screening nature makes the filter-chip and detection system ideal for tailoring antibiotic treatment to individual patients by reducing the microbial antibiotic resistance, and improving the survival rate for patients suffering from postoperative infections.
Tsou
Pei-Hsiang
PH
Department of Electrical and Computer Engineering, Texas A&M University, Zachry Engineering Center, College Station, TX 77843, USA.
Sreenivasappa
Harini
H
Hong
Sungmin
S
Yasuike
Masayuki
M
Miyamoto
Hiroshi
H
Nakano
Keiyo
K
Misawa
Takeyuki
T
Kameoka
Jun
J
D000537:Aluminum Oxide / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D055096:Optical Devices; D014462:Ultrafiltration / Q000295:instrumentation*
D000900:Anti-Bacterial Agents; D000537:Aluminum Oxide