Identification and characterization of nematode specific protective epitopes of Brugia malayi TRX towards development of synthetic vaccine construct for lymphatic filariasis.
Although multi-epitope vaccines have been evaluated for various diseases, they have not yet been investigated for lymphatic filariasis. Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXP1 was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis.
Madhumathi
Jayaprakasam
J
Centre for Biotechnology, Anna University, Sardar Patel Road, Chennai 600025, Tamil Nadu, India.
Prince
Prabhu Rajaiah
PR
Anugraha
Gandhirajan
G
Kiran
Pote
P
Rao
Donthamsetty Nageswara
DN
Reddy
Maryada Venkata Rami
MV
Kaliraj
Perumal
P
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D017178:Brugia malayi / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051189:Murinae; D013601:T-Lymphocytes / Q000276:immunology; D013879:Thioredoxins / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000276:immunology*
D000909:Antibodies, Helminth; D016207:Cytokines; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D018074:Vaccines, Conjugate; D013879:Thioredoxins