Planosporangium mesophilum sp. nov., isolated from rhizosphere soil of Bletilla striata.
A Gram-stain-positive, non-motile actinomycete, designated strain YIM 48875(T), was isolated from rhizosphere soil of Bletilla striata and its taxonomic position was established by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 48875(T) belonged to the genus Planosporangium, supported by a bootstrap value of 100 %. Cells of strain YIM 48875(T) showed two kinds of sporangia, which also supported its classification in the genus Planosporangium. Strain YIM 48875(T) grew optimally at 28 °C, at pH 6.0-8.0 and in the presence of 2 % (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain YIM 48875(T) and Planosporangium flavigriseum YIM 46034(T) was 98.6 %. Strain YIM 48875(T) exhibited a quinone system with menaquinones MK-9(H(4)), MK-9(H(6)) and MK-9(H(8)) as the predominant compounds, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol mannoside and the major fatty acids iso-C(15 : 0) and iso-C(16 : 0); these data were markedly different from those for P. flavigriseum YIM 46034(T). The level of DNA-DNA relatedness between strain YIM 48875(T) and P. flavigriseum YIM 46034(T) was 45.5 %. It is apparent from the genotypic and phenotypic data that strain YIM 48875(T) represents a novel species of the genus Planosporangium, for which the name Planosporangium mesophilum sp. nov. is proposed. The type strain is YIM 48875(T) ( = CCTCC AA 209049(T)  = KCTC 19779(T)).
Cao
Yan-Ru
YR
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.|College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
Wang
Qian
Q
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Jin
Rong-Xian
RX
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Jiang
Yi
Y
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Lai
Hang-Xian
HX
College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
He
Wen-Xiang
WX
College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, The National Engineering Research Center for Microbial Pharmaceutical, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D029595:Orchidaceae / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride