Identification of stem-like cells in head and neck cancer cell lines.
This study investigated the existence of stem-like cells in established head and neck squamous cell carcinoma (HNSCC) lines, HSC3 and HSC4. Flow cytometric analysis confirmed the presence of side population (SP) cells excluding Hoechst 33342 in HSC4 cells (0.37+/-0.06%) but not HSC3 cells in a reserpine-sensitive manner. After sorting, the SP cells generated both SP and main population (MP) cells in culture while MP cells generated MP cells only. Higher expression of stem cell markers was detected in SP than in MP cells. These results suggest that cancer stem-like cells exist in head and neck squamous cell carcinoma.
Sun
Guangwei
G
Division of Hearing and Balance Research, National Institute of Sensory Organs, National Tokyo Medical Center, Meguro, Tokyo 152-8902, Japan.
Fujii
Masato
M
Sonoda
Akira
A
Tokumaru
Yutaka
Y
Matsunaga
Tatsuo
T
Habu
Noboru
N
D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis; D014411:Neoplastic Stem Cells / Q000473:pathology*; D051176:beta Catenin / Q000032:analysis
C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D009363:Neoplasm Proteins; D051176:beta Catenin