In vitro anti-aging activities of Terminalia chebula gall extract.
BACKGROUND
The Thai Lanna region has its own folklores and wisdoms in various fields such as traditional medicines. The galls of Terminalia chebula Retz. (Combretaceae) frequently appear in many Thai Lanna medicinal plant recipes for promoting longevity.


OBJECTIVE
To investigate the in vitro anti-aging activities of the extracts from 15 plants including T. chebula gall selected from the Thai medicinal plant recipes that have been traditionally used for longevity.


METHODS
The plant extracts were prepared by four extraction methods including hot (HW) and cold (CW) aqueous processes and hot (HM) and cold (CM) methanol processes. These extracts were tested for antioxidative and tyrosinase inhibition activity as well as the proliferative and MMP-2 inhibition activity on early aging human skin fibroblasts in order to evaluate their in vitro anti-aging activity.


RESULTS
At 0.1 mg/mL, the CW extract of T. chebula gall exhibited the highest DPPH radical scavenging activity with scavenging of 84.64% +/- 2.22%, whereas ascorbic acid, alpha-tocopherol and butylated hydroxyl toluene gave 96.50% +/- 0.1%, 35.74% +/- 0.2% and 27.43% +/- 0.1%, respectively. The CW extract of T. chebula gall indicated the highest stimulation index (SI) on normal human fibroblast proliferation of 1.441 which was more active than ascorbic acid (SI 1.21). This extract has also demonstrated MMP-2 inhibition on fibroblasts determined by zymography 1.37 times more potent than ascorbic acid.


CONCLUSIONS
This study has confirmed the traditional use of T. chebula gall in many Thai medicinal plant recipes for longevity which will be beneficial for further development of anti-aging products.
Manosroi
Aranya
A
Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. pmpti005@chiangmai.ac.th
Jantrawut
Pensak
P
Akihisa
Toshihiro
T
Manosroi
Worapaka
W
Manosroi
Jiradej
J
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D008518:Medicine, East Asian Traditional; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D015595:Skin Aging / Q000187:drug effects*; D029766:Terminalia / Q000737:chemistry*; D013785:Thailand
D000975:Antioxidants; D005609:Free Radicals; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D014442:Monophenol Monooxygenase