title: Human hematopoietic progenitor cells grow faster under rotational laminar flows.
abstract: We report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54-58.5%, and (c) reduced doubling time in their exponential phase by 12-16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells.
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey at Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, México.
authors.forename: G
authors.initials: G
authors.lastname: Murugappan
authors.forename: L M
authors.initials: LM
authors.lastname: Carrillo-Cocom
authors.forename: K E
authors.initials: KE
authors.lastname: Johnson
authors.forename: M T
authors.initials: MT
authors.lastname: González-Barrón
authors.forename: J E
authors.initials: JE
authors.lastname: Moreno-Cuevas
authors.forename: M M
authors.initials: MM
authors.lastname: Alvarez
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans