DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips.
A DNA-bridged strategy is used to facilely conjugate streptavidin (STV) to luminescent semiconductor quantum dots (QDs), which leads to convenient and stable QD-DNA-biotin-STV conjugates that serve as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers with a microfluidic protein chip.
Hu
Mei
M
Laboratory of Advanced Materials, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
He
Yao
Y
Song
Shiping
S
Yan
Juan
J
Lu
Hao-Ting
HT
Weng
Li-Xing
LX
Wang
Lian-Hui
LH
Fan
Chunhai
C
D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000032:analysis; D001710:Biotin / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry*
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D001710:Biotin; D004247:DNA; D019809:Streptavidin