title: Transmission electron microscopy (TEM) of plant tissues.
abstract: Transmission electron microscopy (TEM) requires extensive preparation of plant tissues to achieve optimal results. Samples must be fixed to preserve ultrastructure, dehydrated to withstand the high vacuum of an electron microscope, and embedded in a suitable resin to permit very thin sections to be produced. This protocol provides details for fixation, dehydration with ethanol, and embedding in Spurr's, an epoxide resin with low viscosity that is well-suited for plant tissues.
authors.forename: Martin
authors.initials: M
authors.lastname: Hülskamp
authors.forename: Birgit
authors.initials: B
authors.lastname: Schwab
authors.forename: Paul
authors.initials: P
authors.lastname: Grini
authors.forename: Heinz
authors.initials: H
authors.lastname: Schwarz
mesh_terms: D003681:Dehydration; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D010944:Plants / Q000648:ultrastructure*; D016610:Tissue Embedding; D016707:Tissue Fixation