Evaluation of various PCR assays for the detection of emetic toxin producing Bacillus cereus.
Because conventional methods for detecting emetic-toxin-producing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxin-producing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the species-specific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.
Kim
Jung-Beom
JB
Division of Health Research & Planning, Gyeonggi-do Research Institute of Health & Environment, Pajang-dong 324-1, Suwon, Gyeonggi-do, Republic of Korea.
Kim
Jae-Myung
JM
Park
Yong-Bae
YB
Han
Jeong-A
JA
Lee
Soon-Ho
SH
Kwak
Hyo-Sun
HS
Hwang
In-Gyun
IG
Yoon
Mi-Hye
MH
Lee
Jong-Bok
JB
Oh
Deog-Hwan
DH
D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D047630:Depsipeptides / Q000096:biosynthesis* / Q000235:genetics; D005516:Food Microbiology; D007723:Korea; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D047630:Depsipeptides; C089294:cereulide