title: The induction of tuftelin expression in PC12 cell line during hypoxia and NGF-induced differentiation.
abstract: The tuftelin protein isoforms undergo post-translation modifications, and are ubiquitously expressed in various tissues in embryos, adults, and tumors. Developmental and pathological studies suggested an apparent correlation between oxygen deprivation and tuftelin expression. The aim of the study was therefore to investigate the effect of a pathological insult (hypoxia) and a physiological growth factor (NGF), which antagonistically regulate HIF1 expression, on tuftelin expression using the neuronal PC12 cell model. In the present study, we first demonstrated the expression of tuftelin in PC12 cells, providing an experimental system to investigate the pathophysiological role of tuftelin. Furthermore, we demonstrated the induction of tuftelin during hypoxia by oxygen deprivation and during chemical hypoxia by cobalt chloride. Down-regulation of HIF1α mRNA blocked hypoxia-induced HIF1α expression, and reduced by 89% hypoxia-induced tuftelin expression. In mice, intraperitoneal injection of cobalt chloride significantly induced tuftelin mRNA and protein expression in the brain. During NGF-mediated PC12 differentiation, tuftelin expression was significantly induced in correlation with neurite outgrowth. This induction was partially blocked by K252a, a selective antagonist of the NGF receptor TrkA, indicating the involvement of the TrkA-signaling pathways in tuftelin induction by NGF. Revealing the physiological role of tuftelin will clarify mechanisms related to the "hypoxic genome," and NGF-induced neurotrophic and angiogenic effects.
authors.affiliation: Dental Research Laboratory, Faculty of Dental Medicine, Institute of Dental Sciences, The Hebrew University of Jerusalem-Hadassah, Jerusalem, Israel.
authors.forename: Yoav
authors.initials: Y
authors.lastname: Leiser
authors.forename: Nechama
authors.initials: N
authors.lastname: Silverstein
authors.forename: Anat
authors.initials: A
authors.lastname: Blumenfeld
authors.forename: Dekel
authors.initials: D
authors.lastname: Shilo
authors.forename: Amir
authors.initials: A
authors.lastname: Haze
authors.forename: Eli
authors.initials: E
authors.lastname: Rosenfeld
authors.forename: Boaz
authors.initials: B
authors.lastname: Shay
authors.forename: Rinat
authors.initials: R
authors.lastname: Tabakman
authors.forename: Shimon
authors.initials: S
authors.lastname: Lecht
authors.forename: Philip
authors.initials: P
authors.lastname: Lazarovici
authors.forename: Dan
authors.initials: D
authors.lastname: Deutsch
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D003035:Cobalt / Q000633:toxicity; D003746:Dental Enamel Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020932:Nerve Growth Factor / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology; D010101:Oxygen Consumption / Q000502:physiology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D051381:Rats; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D003746:Dental Enamel Proteins; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C038456:tuftelin; D003035:Cobalt; D020932:Nerve Growth Factor; D020917:Receptor, trkA; C018021:cobaltous chloride; D010100:Oxygen