High percentage of resistance to ciprofloxacin and qnrB19 gene identified in urinary isolates of extended-spectrum beta-lactamase-producing Escherichia coli in Madrid, Spain.
The presence of qnr genes in 191 extended-spectrum beta-lactamase-producing Escherichia coli from 2005, with 75% of resistance to ciprofloxacin, was evaluated. An SHV-12-producing E. coli carried qnrB19; both genes were transferred by conjugation. No qnrA- or qnrS-positive strains were detected. In addition, we identified 3 new parC mutations (S80W, E84R, and E84A).
Ríos
Esther
E
Servicio de Microbiología, Hospital Clínico San Carlos, 28040 Madrid, Spain. bioesther@hotmail.com
Rodríguez-Avial
Iciar
I
Rodríguez-Avial
Carmen
C
Hernandez
Elena
E
Picazo
Juan Jose
JJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D003227:Conjugation, Genetic; D027101:DNA Topoisomerase IV / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D013030:Spain; D014552:Urinary Tract Infections / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin; D001618:beta-Lactamases; D027101:DNA Topoisomerase IV