A guide to super-resolution fluorescence microscopy.
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.
Schermelleh
Lothar
L
Department of Biology and Center for Integrated Protein Science, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany. lothar.schermelleh@lmu.de
Heintzmann
Rainer
R
Leonhardt
Heinrich
H
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes