Recombinant proteins in the diagnosis of toxoplasmosis.
Toxoplasma gondii is an important human pathogen with a worldwide distribution. It is primarily of medical importance for pregnant women and immunocompromised patients. Primary infection of the former is often associated with fetal infection, which can lead to abortion or severe neonatal malformation. Immunocompromised patients are at risk of contracting the severe form of the disease that may be fatal. Thus, detection of T. gondii infection with high sensitivity and specificity is crucial in the management of the disease. Toxoplasmosis is generally diagnosed by demonstrating specific immunoglobulin M (IgM) and IgG antibodies to toxoplasma antigens in the patient's serum sample. Most of the commercially available tests use T. gondii native antigens and display wide variations in test accuracy. Recombinant antigens have great potential as diagnostic reagents for use in assays to detect toxoplasmosis. Thus in this review, we address recent advances in the use of Toxoplasma recombinant proteins for serodiagnosis of toxoplasmosis.
Kotresha
Dupadahalli
D
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia.
Noordin
Rahmah
R
D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008565:Membrane Proteins / Q000276:immunology; D011247:Pregnancy; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests; D014123:Toxoplasmosis / Q000175:diagnosis*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C106990:Gra2 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D008565:Membrane Proteins; D015800:Protozoan Proteins; C117001:ROP 1 protein, Toxoplasma gondii; D011994:Recombinant Proteins; C047168:SAG1 antigen, Toxoplasma; C067547:surface antigen P22, Toxoplasma