title: The acellular matrix (ACM) for bladder tissue engineering: A quantitative magnetic resonance imaging study.
abstract: Bladder acellular matrices (ACMs) derived from natural tissue are gaining increasing attention for their role in tissue engineering and regeneration. Unlike conventional scaffolds based on biodegradable polymers or gels, ACMs possess native biomechanical and many acquired biologic properties. Efforts to optimize ACM-based scaffolds are ongoing and would be greatly assisted by a noninvasive means to characterize scaffold properties and monitor interaction with cells. MRI is well suited to this role, but research with MRI for scaffold characterization has been limited. This study presents initial results from quantitative MRI measurements for bladder ACM characterization and investigates the effects of incorporating hyaluronic acid, a natural biomaterial useful in tissue-engineering and regeneration. Measured MR relaxation times (T(1), T(2)) and diffusion coefficient were consistent with increased water uptake and glycosaminoglycan content observed on biochemistry in hyaluronic acid ACMs. Multicomponent MRI provided greater specificity, with diffusion data showing an acellular environment and T(2) components distinguishing the separate effects of increased glycosaminoglycans and hydration. These results suggest that quantitative MRI may provide useful information on matrix composition and structure, which is valuable in guiding further development using bladder ACMs for organ regeneration and in strategies involving the use of hyaluronic acid.
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. hai-ling.cheng@sickkids.ca
authors.forename: Hai-Ling Margaret
authors.initials: HL
authors.lastname: Cheng
authors.forename: Yasir
authors.initials: Y
authors.lastname: Loai
authors.forename: Marine
authors.initials: M
authors.lastname: Beaumont
authors.forename: Walid A
authors.initials: WA
authors.lastname: Farhat
mesh_terms: D000818:Animals; D002474:Cell-Free System / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D001743:Urinary Bladder / Q000033:anatomy & histology* / Q000737:chemistry*
chemical_list: D017886:Durapatite