Modeling the electrophoresis and transport of peptides: the effective sphere model and complex formation.
Modeling the electrophoretic mobility of peptides is examined in this study using a "coarse grained" bead model, or B model for short 8 and also a simpler "effective sphere" (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013 M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.
Allison
Stuart A
SA
Department of Chemistry, Georgia State University, Atlanta, GA 30302-4098, USA sallison@gsu.edu
Pei
Hongxia
H
Allen
Michelle
M
Brown
Jocelyn
J
Kim
Chang-Il
CI
Zhen
Yang
Y
D001692:Biological Transport; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010455:Peptides / Q000302:isolation & purification*
D010455:Peptides