Localization of potential ion transport pathways in vesicular trichome cells of Atriplex halimus L.
The secreting glandular trichomes are recognized as an efficient structure that alleviates salt effects on Atriplex halimus. They are found on buds, young green stems, and leaves. They occupy both the leaf surfaces and give them a whitish color. Their histogenesis and ultrastructure were investigated in the third young leaves. They appear in early stage of plant development and its initiation continuous until just the leaf final development state. Each trichome contains two parts; a stalk which has high electron opacity, embedded in epidermal cells, and bears a second one which is unicellular, called bladder cell and has a low electron density. The bladder cell appears as a huge vacuole and the well-reduced cytoplasm which is pushed close to the wall, contains only a few organelles. Concurrently, the use of silver chloride precipitation technique shows that, in secretion process, salt follows a symplasmatic pathway which is consolidated by the presence of numerous plasmodesmata between the stalk cell(s), and the bladder one and the neighboring mesophyll cells. In addition, according to lanthanum-tracer study, salt can be excreted apoplastically. In fact, the heavy element can be transported via endocytosis vesicles, and by Golgi, endoplasmic reticulum, and lysosome (G.E.R.L.) network toward the storage vacuoles.
Smaoui
Abderrazak
A
Laboratoire des Plantes Extremôphiles, Centre de Biotechnologie de la Technopole de Borj-Cedria, BP 901, Hammam-Lif, 2050, Tunisia.
Barhoumi
Zouhaier
Z
Rabhi
Mokded
M
Abdelly
Chedly
C
D027466:Atriplex / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D001343:Autophagy; D004705:Endocytosis; D017136:Ion Transport / Q000187:drug effects; D007811:Lanthanum / Q000494:pharmacology; D008854:Microscopy, Electron; D012492:Salts / Q000378:metabolism; D018030:Silver Compounds / Q000494:pharmacology; D022161:Transport Vesicles / Q000378:metabolism*
D012492:Salts; D018030:Silver Compounds; D007811:Lanthanum; C037548:silver chloride