Development of a tandem time-of-flight mass spectrometer with an electrospray ionization ion source.
A new tandem time-of-flight mass spectrometer with an electrospray ionization ion source 'ESI-TOF/quadTOF' was designed and constructed to achieve the desired aim of structural elucidation via high-energy collision-induced dissociation (CID), and the simultaneous detection of all fragment ions. The instrument consists of an orthogonal acceleration-type ESI ion source, a linear TOF mass spectrometer, a collision cell, a quadratic-field ion mirror and a microchannel plate detector. High-energy CID spectra of doubly protonated angiotensin II and bradykinin were obtained. Several fragment ions such as a-, d-, v- and w-type ions, characteristic of high-energy CID, were clearly observed in these spectra. These high-energy CID fragment ions enabled confirmation of the complete sequence, including leucine-isoleucine determinations. It was demonstrated that high-energy CID of multiply protonated peptides could be achieved in the ESI-TOF/quadTOF.
Nagao
Hirofumi
H
Renovation Center of Instruments for Science Education and Technology, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. nagao@mass.phys.sci.osaka-u.ac.jp
Shimma
Shuichi
S
Hayakawa
Shigeo
S
Awazu
Kunio
K
Toyoda
Michisato
M
D000804:Angiotensin II / Q000737:chemistry; D001920:Bradykinin / Q000737:chemistry; D004867:Equipment Design; D007532:Isoleucine / Q000737:chemistry; D007930:Leucine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*
D010455:Peptides; D007532:Isoleucine; D000804:Angiotensin II; D007930:Leucine; D001920:Bradykinin