Canarypox and fowlpox viruses as recombinant vaccine vectors: a biological and immunological comparison.
Canarypox and fowlpox viruses represent alternative vaccine vectors due to their natural host-range restriction to avian species. Although they cannot replicate in mammals, they correctly express transgenes in human cells and elicit a complete immune response in vaccinated subjects. Several studies have evaluated their genomic differences and protective efficacy in preclinical trials, but detailed information is not available for their transgene expression, cytokine modulation and abortive replication in mammals. This study demonstrates that the heterologous HIV gag/pol and env genes are more efficiently expressed by fowlpox in non-immune and immune cells. The production of retrovirus-like particles, the longer transgene expression, and a balanced cytokine induction may confer to fowlpox-based recombinants the ability to elicit a better immune response.
Zanotto
Carlo
C
Department of Medical Pharmacology, Università di Milano, 20129 Milan, Italy. carlo.zanotto@unimi.it
Pozzi
Eleana
E
Pacchioni
Sole
S
Volonté
Luca
L
De Giuli Morghen
Carlo
C
Radaelli
Antonia
A
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology; D000818:Animals; D018122:B7-1 Antigen / Q000032:analysis; D028121:Canarypox virus / Q000235:genetics / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D015870:Gene Expression; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007114:Immunization; D008264:Macrophages / Q000276:immunology; D046529:Microscopy, Electron, Transmission; D019076:Transgenes; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014779:Virus Replication / Q000235:genetics
D016915:AIDS Vaccines; D018122:B7-1 Antigen; D016207:Cytokines; D014614:Vaccines, Synthetic