Immunogenic dendritic cells primed by social defeat enhance adaptive immunity to influenza A virus.
Dendritic cells (DCs) sample their surrounding microenvironment and consequently send immunogenic or regulatory signals to T cells during DC/T cell interactions, shaping the primary adaptive immune response to infection. The microenvironment resulting from repeated social defeat increases DC co-stimulatory molecule expression and primes DCs for enhanced cytokine responses in vitro. In this study, we show that social disruption stress (SDR) results in the generation of immunogenic DCs, capable of conferring enhanced adaptive immunity to influenza A/PR/8/34 infection. Mice infected with influenza A/PR/8/34 virus 24 h after the adoptive transfer of DCs from SDR mice had significantly increased numbers of D(b)NP(366-74)CD8(+) T cells, increased IFN-γ and IFN-α mRNA, and decreased influenza M1 mRNA expression in the lung during the peak primary response (9 days post-infection), compared to mice that received DCs from naïve mice. These data demonstrate that repeated social defeat is a significant environmental influence on immunogenic DC activation and function.
Powell
Nicole D
ND
Section of Oral Biology, College of Dentistry, The Ohio State University, Columbus, OH, USA. Nicole.Powell@osumc.edu
Mays
Jacqueline W
JW
Bailey
Michael T
MT
Hanke
Mark L
ML
Sheridan
John F
JF
D056704:Adaptive Immunity / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D018845:CD11 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D003163:Competitive Behavior / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology*; D016898:Interferon-alpha / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012930:Social Dominance; D012931:Social Environment; D013315:Stress, Psychological / Q000276:immunology
D000956:Antigens, Viral; D018845:CD11 Antigens; D000939:Epitopes; D016898:Interferon-alpha; D007371:Interferon-gamma