Effect of Hemidesmus indicus on ischemia-reperfusion injury in the isolated rat heart.
The root extract of Hemidesmus indicus (Linn.) R. Br. (Asclepiadaceae) (HI) was studied for its cardioprotective effect in Langendorff-perfused rat hearts. HI was perfused for 15 min at a concentration of 0.09 g/L prior to 30 min global ischemia/120 min reperfusion (I/R). Recovery of functional parameters, reperfusion arrhythmias, and infarct size (TTC staining) served as the end-points. After 15 min of perfusion with HI, the left ventricular developed pressure (LVdevP) and HR (heart rate) were not altered significantly (p>0.05), as compared with the pre-drug values. During R, HI showed a significantly higher (p<0.05) recovery of LVdevP at nearly all time points. The recovery of maximal rate of pressure development (+dP/dtmax) and left ventricular end-diastolic pressure (LVEDP) at 40 min of R were significantly better than in non-treated controls. There was also a significant reduction in the total number of ventricular premature beats (VPB) and duration of ventricular tachycardia (VT). HI can protect ischemic myocardium against contractile dysfunction and reperfusion-induced arrhythmias and reduce the extent of irreversible tissue damage following I/R in rat hearts.
Khandelwal
V K M
VK
Institute for Heart Research, Center of Excellence for Cardiovascular Research of the Slovak Academy of Sciences, Bratislava, Slovakia.
Balaraman
R
R
Ondrejcáková
M
M
Pancza
D
D
Ravingerová
T
T
D000818:Animals; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D031102:Hemidesmus / Q000737:chemistry*; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D009203:Myocardial Infarction / Q000209:etiology / Q000517:prevention & control; D015428:Myocardial Reperfusion Injury / Q000150:complications*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D017180:Tachycardia, Ventricular / Q000209:etiology / Q000517:prevention & control; D013997:Time Factors; D018879:Ventricular Premature Complexes / Q000209:etiology / Q000517:prevention & control
D002316:Cardiotonic Agents; D010936:Plant Extracts