title: Decolorization of dye Reactive Black 5 by newly isolated thermophilic microorganisms from geothermal sites in Galicia (Spain).
abstract: In this study, thermophilic microbial strains from thermal spots in northwestern Spain displaying excellent decolorization capability were isolated. The research work tackled: (i) the ability of consortia to degrade a model di-azo dye Reactive Black at different pHs in flask cultures, obtaining that just neutral pHs licensed degradation levels near to 70%, (ii) the isolation of tree of the bacteria, which rendered possible reaching high levels of decolorization (80%) after just 24 h in aerobic conditions, and which were identified through 16S rRNA sequencing to possess high homology (99%) with Anoxybacillus pushchinoensis, Anoxybacillus kamchatkensis and Anoxybacillus flavithermus, and (iii) the cultivation of the isolates in a bench-scale bioreactor, which led to a decolorization rate two-fold higher than that obtained in flask cultures. Therefore, this work makes up the first time that a decolorization process of an azo dye by thermophilic microorganisms in aerobic conditions is investigated.
authors.affiliation: Department of Chemical Engineering, University of Vigo, 36310 Vigo, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Deive
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.forename: T
authors.initials: T
authors.lastname: Barrio
authors.forename: F
authors.initials: F
authors.lastname: Moscoso
authors.forename: P
authors.initials: P
authors.lastname: Morán
authors.forename: M A
authors.initials: MA
authors.lastname: Longo
authors.forename: M A
authors.initials: MA
authors.lastname: Sanromán
mesh_terms: D058677:Anoxybacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D003116:Color; D009282:Naphthalenesulfonates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013030:Spain
chemical_list: D009282:Naphthalenesulfonates; D012336:RNA, Ribosomal, 16S; C063421:Remazol black B