title: High-throughput in vivo vertebrate screening.
abstract: We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
authors.affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pardo-Martin
authors.forename: Tsung-Yao
authors.initials: TY
authors.lastname: Chang
authors.forename: Bryan Kyo
authors.initials: BK
authors.lastname: Koo
authors.forename: Cody L
authors.initials: CL
authors.lastname: Gilleland
authors.forename: Steven C
authors.initials: SC
authors.lastname: Wasserman
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Yanik
mesh_terms: D000818:Animals; D001331:Automation; D001369:Axons; D004625:Embryo, Nonmammalian; D004867:Equipment Design; D053685:Laser Therapy; D008403:Mass Screening / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D023421:Models, Animal; D009154:Mutation; D012160:Retina / Q000166:cytology*; D015027:Zebrafish