Two novel coding SNPs of SREBP1c gene are associated with body weight and average daily gain in bovine.
It is known that the SREBP1c gene is an important gene responsible for adipogenesis and regulation of the expression of genes controlling fatty acid biosynthesis. Its expression levels increase in parallel with obesity. Therefore, the present study focused on screening the genetic variation within bovine SREBP1c gene and analyzing its effect on growth traits in 1035 individuals belonging to four Chinese cattle breeds (QC, NY, JX, CH) using PCR-SSCP, DNA sequencing, and forced PCR-RFLP methods. The results revealed two novel mutations: NC_007317: g. 10781 C > A (457aa), 10914 G > A (502aa). Association analysis with growth traits in the Nangyang breed indicated that: The SNPs in the bovine SREBP1c gene had significant effects on body weight and average daily gain at birth, 6 and 12 months old (P < 0.05 or P < 0.01). Therefore, these results suggest that the SREBP1c gene is a strong candidate gene that affects growth traits in cattle.
Huang
Yongzhen
Y
Northwest A&F University, Yangling, China. huangyongzhen2009@yahoo.com.cn
Zhang
Enping
E
Wang
Jing
J
Huai
Yongtao
Y
Lan
Xianyong
X
Ma
Liang
L
Li
Zhuanjian
Z
Ren
Gang
G
Chen
Fuying
F
Lei
Chuzhao
C
Wang
Juqiang
J
Chen
Hong
H
D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000502:physiology*; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D016009:Chi-Square Distribution; D004247:DNA / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics* / Q000502:physiology
D051780:Sterol Regulatory Element Binding Protein 1; D004247:DNA