Hedgehog signaling regulates segment formation in the annelid Platynereis.
Annelids and arthropods share a similar segmented organization of the body whose evolutionary origin remains unclear. The Hedgehog signaling pathway, prominent in arthropod embryonic segment patterning, has not been shown to have a similar function outside arthropods. We show that the ligand Hedgehog, the receptor Patched, and the transcription factor Gli are all expressed in striped patterns before the morphological appearance of segments in the annelid Platynereis dumerilii. Treatments with small molecules antagonistic to Hedgehog signaling disrupt segment formation. Platynereis Hedgehog is not necessary to establish early segment patterns but is required to maintain them. The molecular similarity of segment patterning functions of the Hedgehog pathway in an annelid and in arthropods supports a common origin of segmentation in protostomes.
Dray
Nicolas
N
Centre de Génétique Moléculaire du CNRS, FRE 3144, Avenue de la Terrasse, 91189 Gif-sur-Yvette, France.
Tessmar-Raible
Kristin
K
Le Gouar
Martine
M
Vibert
Laura
L
Christodoulou
Foteini
F
Schipany
Katharina
K
Guillou
Aurélien
A
Zantke
Juliane
J
Snyman
Heidi
H
Béhague
Julien
J
Vervoort
Michel
M
Arendt
Detlev
D
Balavoine
Guillaume
G
D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005075:Biological Evolution; D019521:Body Patterning / Q000187:drug effects; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D000072079:Patched Receptors; D010802:Phylogeny; D010879:Piperazines / Q000494:pharmacology; D011077:Polychaeta / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014704:Veratrum Alkaloids / Q000494:pharmacology
D053823:Hedgehog Proteins; D000072079:Patched Receptors; D010879:Piperazines; D011720:Pyrazoles; D011956:Receptors, Cell Surface; C468919:SANT-1 compound; D014157:Transcription Factors; D014704:Veratrum Alkaloids; C000541:cyclopamine