Anti-obesity effect of Morus bombycis root extract: anti-lipase activity and lipolytic effect.
OBJECTIVE
This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues.


METHODS
Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured.


RESULTS
Morus bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07 microg/mL.In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner.


CONCLUSIONS
The present study suggests that Morus bombycis root extract might be of therapeutic interest with respect to the treatment of obesity.
Kim
Young Sook
YS
Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon, Republic of Korea.
Lee
Yun Mi
YM
Kim
Hyojun
H
Kim
Junghyun
J
Jang
Dae Sik
DS
Kim
Joo Hwan
JH
Kim
Jin Sook
JS
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005990:Glycerol / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D031625:Morus / Q000737:chemistry*; D009765:Obesity / Q000188:drug therapy; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D019440:Anti-Obesity Agents; D010726:Phosphodiesterase Inhibitors; D010936:Plant Extracts; D014280:Triglycerides; D010727:Phosphoric Diester Hydrolases; D005990:Glycerol