title: In situ functionalization of wet-spun fibre meshes for bone tissue engineering.
abstract: Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (Si-OH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet-spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet-spun fibre meshes with Si-OH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet-spinning, using a calcium silicate solution as a non-solvent and functionalized in situ with Si-OH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL-Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that Si-OH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet-spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo.
authors.affiliation: 3Bs Research Group-Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Guimarães, Portugal. belinha@dep.uminho.pt
authors.forename: Isabel B
authors.initials: IB
authors.lastname: Leonor
authors.forename: Márcia T
authors.initials: MT
authors.lastname: Rodrigues
authors.forename: Manuela E
authors.initials: ME
authors.lastname: Gomes
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D017610:Calcium Compounds; D049109:Cell Proliferation; D002470:Cell Survival; D006041:Goats; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D011091:Polyesters; D017640:Silicates; D013213:Starch; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D017610:Calcium Compounds; D011091:Polyesters; D017640:Silicates; C016240:polycaprolactone; D013213:Starch; C031293:calcium silicate