Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle.
The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis.


OBJECTIVE
Hence, this study investigated if surface modifications with plasma treatments could reduce the adherence of C. albicans to a denture base resin.


METHODS
Specimens (n=180) with roughened and smooth surfaces were made and divided into five groups: control-specimens were left untreated; experimental groups-specimens were submitted to plasma treatments to obtain surfaces with different hydrophobicity (Ar/50W; ArO2/70W; AAt/130W) or incorporation of fluorine (Ar/SF(6)70W). Contact angle measurements were performed immediately after the treatments and after immersion in water for 48h. For each group, half of the specimens were incubated with saliva prior to the adhesion assay. The number of adherent yeasts was evaluated by XTT reduction method.


RESULTS
For the experimental groups, there was significant change in the mean contact angle after 48h of immersion in water. Groups ArO2/70W and ArSF6/70W showed significantly lower absorbance readings than the other groups, regardless the presence or absence of saliva and surface roughness.


CONCLUSIONS
Results demonstrated that ArO2/70W and ArSF6/70W plasma treatments showed promising potential for reducing the adherence of C. albicans to denture base resins.
Zamperini
Camila Andrade
CA
Araraquara Dental School, UNESP - Univ Estadual Paulista, Department of Dental Materials and Prosthodontics, Araraquara, São Paulo, Brazil.
Machado
Ana Lucia
AL
Vergani
Carlos Eduardo
CE
Pavarina
Ana Claudia
AC
Giampaolo
Eunice Terezinha
ET
da Cruz
Nilson Cristino
NC
D000180:Acrylic Resins; D000328:Adult; D001128:Argon; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D004396:Coloring Agents; D044622:Dental Pellicle / Q000502:physiology; D003819:Denture Bases; D006801:Humans; D058626:Plasma Gases / Q000494:pharmacology*; D013499:Surface Properties; D013778:Tetrazolium Salts; D017155:Wettability
D000180:Acrylic Resins; D004396:Coloring Agents; D058626:Plasma Gases; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D001128:Argon