title: Application of non-invasive biomarkers in a birth cohort follow-up in relation to respiratory health outcome.
abstract: Asthma-related symptoms can manifest in children during the early years, but only some of the children will develop the disease. This feasibility study showed that it is possible to apply non-invasive markers (in urine, exhaled nitric oxide (FENO) and exhaled breath condensate (EBC)) in 3-year-old children, and evaluated the biomarkers in relation to health outcomes and potential modifiers. FENO was correlated with respiratory allergy, and was borderline significantly correlated with wheezing, but not with the asthma predictive index (mAPI). EBC pH and urinary 8-oxo-deoxyguanosine were not significantly correlated with these clinical outcomes. An EBC proteolytic peptide pattern was developed, which could distinguish between mAPI-positive and -negative children. Non-invasive biomarkers may become a promising tool for investigating respiratory health in children but further research is needed.
authors.affiliation: Flemish Institute for Technological Research, Boeretang, Mol, Belgium. karolien.bloemen@vito.be
authors.forename: Karolien
authors.initials: K
authors.lastname: Bloemen
authors.forename: Gudrun
authors.initials: G
authors.lastname: Koppen
authors.forename: Eva
authors.initials: E
authors.lastname: Govarts
authors.forename: Ann
authors.initials: A
authors.lastname: Colles
authors.forename: Rosette
authors.initials: R
authors.lastname: Van Den Heuvel
authors.forename: Vera
authors.initials: V
authors.lastname: Nelen
authors.forename: Erwin
authors.initials: E
authors.lastname: Witters
authors.forename: Kristine
authors.initials: K
authors.lastname: Desager
authors.forename: Greet
authors.initials: G
authors.lastname: Schoeters
mesh_terms: D001249:Asthma / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D001944:Breath Tests; D015331:Cohort Studies; D005500:Follow-Up Studies; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009569:Nitric Oxide / Q000378:metabolism
chemical_list: D015415:Biomarkers; D009569:Nitric Oxide