title: [Effect and mechanism of methyl protodioscin in protecting cardiomyocytes against anoxia/reoxygenation injury].
abstract: OBJECTIVE
  To study the effect and mechanism of methyl protodioscin (MPD), an active ingredients of yamogenin, in protecting cardiomyocytes (CMC) against anoxia/reoxygenation (A/R) injury.
  
  
  METHODS
  Cultured CMCs of neonatal SD rats were randomly divided into three groups, cells in Group A were untreated normal cells, cells in Group B and C were made to injury CMC model by A/R, and only those in Group C were treated with MPD. Levels of ATPase activity and lactate dehydrogenase (LDH) in cell membrane of CMCs were determined. Besides, the mRNA expression of sodium-calcium exchanger (NCX) in MPD treated CMCs was detected.
  
  
  RESULTS
  As compared with Group B, the degree of CMC injury was significantly milder and the activities of Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase were higher in Group C after cells were treated with MPD in concentration of 10 microg/mL and 50 microg/mL. The mRNA expression of NCX in CMCs was down-regulated after MPD treatment (P < 0.05).
  
  
  CONCLUSIONS
  MPD could maintain the low calcium internal environment in CMCs by way of protecting the membranous function of Na+ -pump and Ca2+ -pump, and influencing the Ca2+ transmembrane transportation in CMCs.
authors.affiliation: The First Affiliated Hospital of Guangxi Medical University, Nanning. gxningzong@yahoo.com.cn
authors.forename: Zong
authors.initials: Z
authors.lastname: Ning
authors.forename: Yi-kui
authors.initials: YK
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*
chemical_list: D012503:Saponins; C425324:methyl protodioscin; D004144:Diosgenin; C514916:yamogenin; D010100:Oxygen