title: Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B.
abstract: The producers of glycolipid biosurfactant, mannosylerythritol lipid-B (MEL-B), were isolated from leaves of Perilla frutescens on Ibaraki in Japan. Four isolates, 1D9, 1D10, 1D11, and 1E5, were identified as basidiomycetous yeast Pseudozyma tsukubaensis by rDNA sequence and biochemical properties. The structure of MEL-B produced by these strains was analyzed by (1)H nuclear magnetic resonance and gas chromatography-mass spectrometry methods, and was determined to be the same as the diastereomer MEL-B produced by P. tsukubaensis NBRC 1940. Of these isolates, P. tsukubaensis 1E5 (JCM 16987) is capable of producing the largest amount of the diastereomer MEL-B from vegetable oils. In order to progress the diastereomer MEL-B production by strain 1E5, factors affecting the production, such as carbon and organic nutrient sources, were further examined. Olive oil and yeast extract were the best carbon and nutrient sources, respectively. Under the optimal conditions, a maximum yield, productivity, and yield coefficient of 73.1 g/L, 10.4 g L(-1) day(-1), and 43.5 g/g were achieved by feeding of olive oil in a 5-L jar-fermenter culture using strain 1E5.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Masako
authors.initials: M
authors.lastname: Takashima
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Konishi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D006017:Glycolipids / Q000096:biosynthesis*; D007218:Industrial Microbiology; D009682:Magnetic Resonance Spectroscopy; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D013501:Surface-Active Agents / Q000378:metabolism*; D015003:Yeasts / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D006017:Glycolipids; D012331:RNA, Fungal; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid