Transmission electron microscopy (TEM) of plant tissues.
Transmission electron microscopy (TEM) requires extensive preparation of plant tissues to achieve optimal results. Samples must be fixed to preserve ultrastructure, dehydrated to withstand the high vacuum of an electron microscope, and embedded in a suitable resin to permit very thin sections to be produced. This protocol provides details for fixation, dehydration with ethanol, and embedding in Spurr's, an epoxide resin with low viscosity that is well-suited for plant tissues.
Hülskamp
Martin
M
Schwab
Birgit
B
Grini
Paul
P
Schwarz
Heinz
H
D003681:Dehydration; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D010944:Plants / Q000648:ultrastructure*; D016610:Tissue Embedding; D016707:Tissue Fixation