title: [Evaluation of capability of cell count and detection of tumor cells in cerebrospinal and body fluids by automated hematology analyzer].
abstract: Sysmex XE-5000 offers the body fluid modus which provides the opportunity to count and differentiate leukocytes in body fluids and cerebrospinal fluid (CFS). In this study, we evaluated the basic performance of this application using routinely obtained samples in comparison with manual counting. Reproducibility study yielded good results in samples with a high white blood cell (WBC) count, whereas relatively high imprecision was observed at low WBC counts. Linearity was established up to 1,500 cells/microL in CFS and 5,600 cells/microL in body fluid. The cell count by XE-5000 was highly correlated with that of the microscopic reference method. Highly fluorescent body fluid cells percent (HF-BF%) was observed in samples with tumor cells or activated macrophages, which provides information about the possible presence of tumor cells. In conclusion, total and differential WBC counts in body fluid and CFS can be reliably determined by XE-5000 in samples with increased cell counts. XE-5000 also provides screening information about the presence of tumor cells for further manual examination.
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takemura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabe
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ishii
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kuno
authors.forename: Takashi
authors.initials: T
authors.lastname: Horii
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miyake
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
mesh_terms: D001826:Body Fluids / Q000166:cytology*; D002452:Cell Count / Q000295:instrumentation*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D003581:Cytodiagnosis / Q000295:instrumentation*; D006405:Hematology / Q000295:instrumentation*; D006801:Humans; D007962:Leukocytes; D008262:Macrophage Activation; D008264:Macrophages; D009360:Neoplastic Cells, Circulating; D015203:Reproducibility of Results