Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis.
In our recent paper in the Plant Journal, we reported that Arabidopsis thaliana lysophospholipase 2 (lysoPL2) binds acyl-CoA-binding protein 2 (ACBP2) to mediate cadmium [Cd(II)] tolerance in transgenic Arabidopsis. ACBP2 contains ankyrin repeats that have been previously shown to mediate protein-protein interactions with an ethylene-responsive element binding protein (AtEBP) and a farnesylated protein 6 (AtFP6). Transgenic Arabidopsis ACBP2-overexpressors, lysoPL2-overexpressors and AtFP6-overexpressors all display enhanced Cd(II) tolerance, in comparison to wild type, suggesting that ACBP2 and its protein partners work together to mediate Cd(II) tolerance. Given that recombinant ACBP2 and AtFP6 can independently bind Cd(II) in vitro, they may be able to participate in Cd(II) translocation. The binding of recombinant ACBP2 to [(14)C]linoleoyl-CoA and [(14)C]linolenoyl-CoA implies its role in phospholipid repair. In conclusion, ACBP2 can mediate tolerance to Cd(II)-induced oxidative stress by interacting with two protein partners, AtFP6 and lysoPL2. Observations that ACBP2 also binds lysophosphatidylcholine (lysoPC) in vitro and that recombinant lysoPL2 degrades lysoPC, further confirm an interactive role for ACBP2 and lysoPL2 in overcoming Cd(II)-induced stress.
Gao
Wei
W
School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
Li
Hong-Ye
HY
Xiao
Shi
S
Chye
Mee-Len
ML
D000214:Acyl Coenzyme A / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008245:Lysophospholipase / Q000378:metabolism*; D026901:Membrane Transport Proteins; D018384:Oxidative Stress; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding
C419263:ACBP2 protein, Arabidopsis; D000214:Acyl Coenzyme A; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C534910:FP6 protein, Arabidopsis; D008244:Lysophosphatidylcholines; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D002104:Cadmium; C093680:ethylene-responsive element binding protein; C026562:linoleoyl-coenzyme A; D008245:Lysophospholipase