title: A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis.
abstract: This work describes a microfluidic device integrated with multichamber polymerase chain reaction (PCR) and multichannel separation for parallel genetic analysis. The microdevice consists of three functional units: temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel connected to a PCR chamber). Platinum (Pt)/titanium (Ti) microheater was used to ensure homogeneous temperature field, and Pt-chip sensor was used for temperature monitoring. The interface between chip-PCR and chip separation was simplified by connecting the PCR chamber with separation channel directly. After chip-PCR, PCR products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. This microdevice was successfully applied for detection of pathogens including hepatitis B virus (HBV) and Mycobacterium tuberculosis (MTB), and genotyping of human leucocyte antigen (HLA)-B27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pan
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Kaiying
authors.initials: K
authors.lastname: Liu
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010984:Platinum / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
chemical_list: D015796:HLA-B27 Antigen; D010984:Platinum; D014025:Titanium