Suppression of S-phase kinase-associated protein 2 induces apoptosis and inhibits tumor growth in human laryngeal cancer.
S-phase kinase-associated protein 2 (Skp2) is related to cellular proliferation and differentiation in laryngeal carcinoma. This study aimed to investigate the effect of Skp-2 suppression on p27 expression, cellular proliferation, apoptosis, and tumor growth in laryngeal squamous cell carcinoma. Skp2 was stably suppressed in Hep2 laryngeal carcinoma cells by lentivirus siRNA technology and Hep2-siRNA was then inoculated into nude mice. The siRNA-induced suppression of Skp2 increased p27 expression, decreased cellular proliferation, and increased apoptosis in human laryngeal carcinoma cells and tumors, indicating that Skp2 is a viable target in the gene therapy treatment of human laryngeal carcinoma.
Liu
Jiangtao
J
Department of Otorhinolaryngology, China Medical University Affiliated Shengjing Hospital, Shenyang, China.
Tan
Lijun
L
Li
Huijun
H
Wang
Qiwei
Q
Ji
Wenyue
W
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D016133:Polymerase Chain Reaction; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D044786:S-Phase Kinase-Associated Proteins; C413374:p27 antigen