[The quantitative analysis of Ub and UbE1 in focus of traumatic epilepsy].
OBJECTIVE
To study the expression of ubiquitin proteasome system (UPS) in the traumatic epilepsy pathogenesis and its value in traumatic epilepsy by quantitative analysis.


METHODS
Fifteen specimens from human epileptic temporal cortex from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Fifteen normal cerebral cortex specimens died from acute traffic accident were collected as the control group. The expression of mRNA and protein of Ub and UbE1 were detected by RT-PCR and Western blot. Statistical analysis was used to compare the data between three groups.


RESULTS
The expression of mRNA and protein of Ub and UbE1 were the following order: PTE group(high), non-PTE group(middle) and control group(low).


CONCLUSIONS
The study confirms that UPS is up-regulated in the epilepsy's focus, especially in traumatic epilepsy. The activation of UPS may be an important pathological change in neurons in pathogenesis of traumatic epilepsy.
He
Peng-xiang
PX
Department of Forensic Medicine, Zhongshan Medical College, SUN Yat-sen University, Guangzhou 510080, China. seahe7@sina.com.cn
Tang
Yun-lin
YL
Cai
Wei-lin
WL
Huang
Yong-hua
YH
Fang
Chao
C
Jing
Hua-lan
HL
D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D006259:Craniocerebral Trauma / Q000150:complications* / Q000473:pathology; D017931:DNA Primers; D004834:Epilepsy, Post-Traumatic / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D049429:Forensic Pathology; D006801:Humans; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D017931:DNA Primers; D012333:RNA, Messenger; C471538:UBA1 protein, human; D025801:Ubiquitin; D044764:Ubiquitin-Activating Enzymes