Prostanoid extraction and measurement.
Prostanoids are involved in numerous physiological and pathophysiological processes in all body organs. Therefore, measurement of the concentration of the prostanoids has always been of importance for research and drug development purposes as a measure of cyclooxygenase (COX) activities. Techniques used for the measurement of prostanoids have been described decades ago. These techniques have come a long way and improvements have been reported, especially with the specificity in competition immunoassays that rely on the use of specific antibodies against a given prostanoid. These assays are relatively fast and do not involve the use of radioactive isotopes as radioimmunoassay. However, prior extraction is required in order to concentrate the prostanoids and remove interfering substances such as proteins. In this chapter, we describe two protocols for the extraction and measurement of prostanoids using C18 columns and commercial enzyme immunoassays, which do not require specialized equipments and can be performed in any laboratory with standard equipments.
Polenzani
Lorenzo
L
R&D Pipeline Strategy Manager, Angelini Research Center, Santa Palomba-Pomezia, Rome, Italy. l.polenzani@angelini.it
Ayoub
Samir S
SS
D000818:Animals; D001923:Brain Chemistry; D005260:Female; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010537:Peritoneum / Q000737:chemistry; D011453:Prostaglandins / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000737:chemistry
D011453:Prostaglandins