Long-term response of osteogenic cells on micron and submicron-scale-structured hydrophilic titanium surfaces: sequence of cell proliferation and cell differentiation.
OBJECTIVE
Modifications of surface topography and surface chemistry are key factors for guiding target cells during dental implant healing. Recent in vitro studies confirmed promotion of early osteogenic cell differentiation on submicron scaled surfaces in particular when hydrophilized. However, no long-term observations on both osteogenic cell proliferation as well as on cell maturation have been reported for respectively modified surfaces. Aim of this study was to monitor osteogenic cell proliferation and expression of specific osteogenic cell differentiation markers on a protein level over an extended period of 3 weeks with respect to surface modifications.


METHODS
Modified titanium (Ti) disks were obtained from Institute Straumann, representing the following surfaces: smooth pretreatment (PT), sandblasted/acid etched (SLA), and hydrophilized (modSLA). Surface topography was analyzed by scanning electron microscopy, surface elemental composition was assessed by X-Ray Photoelectronic Spectroscopy (XPS). Tissue culture polystyrene (TCPS) served as a control substrate. Human osteogenic cells (HOB-c) were cultivated on the respective substrates. After 24 hrs, 48 hrs, 72 hrs, 7 d, 14 d and 21 d, cell count was assessed as well as osteogenic cell differentiation utilizing cellular Quantitative Immuno-Cytochemistry (QIC) assay for collagen type I (COL), alkaline phosphatase (AP), osteopontin (OPN) and osteocalcin (OC). Data were normalized with respect to internal controls.


RESULTS
In contrast to the other modified Ti disks, modSLA stands out due to low surface carbon contamination. TCPS and PT surfaces preserved a rather immature, mitotic active osteogenic phenotype (high proliferation rates, no increase of OC production), SLA and especially modSLA surfaces promoted the maturation of osteogenic precursors into post-mitotic osteoblasts. In detail, modSLA resulted in lowest cell proliferation rates, but exhibited highest expression rates of OC.


CONCLUSIONS
Our results, which confirm previous studies, reveal long-term promotion of osteogenic cell maturation by topography (micron and submicron scale roughness) and surface hydrophilicity.
Klein
Marcus Oliver
MO
Department of Oral and Maxillofacial Surgery, Medical Faculty, University of Mainz, Mainz, Germany. klein@mkg.klinik.uni-mainz.de
Bijelic
Ana
A
Toyoshima
Takeshi
T
Götz
Hermann
H
von Koppenfels
Roxana Laura
RL
Al-Nawas
Bilal
B
Duschner
Heinz
H
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002244:Carbon / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D024042:Collagen Type I / Q000096:biosynthesis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin / Q000096:biosynthesis; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014025:Titanium; D017155:Wettability
D024042:Collagen Type I; D015675:Osteocalcin; D053495:Osteopontin; D002244:Carbon; D014025:Titanium; D000469:Alkaline Phosphatase