title: DNA-bridged bioconjugation of fluorescent quantum dots for highly sensitive microfluidic protein chips.
abstract: A DNA-bridged strategy is used to facilely conjugate streptavidin (STV) to luminescent semiconductor quantum dots (QDs), which leads to convenient and stable QD-DNA-biotin-STV conjugates that serve as fluorescent nanoprobes for ultrasensitive detection of cancer biomarkers with a microfluidic protein chip.
authors.affiliation: Laboratory of Advanced Materials, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Hu
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.forename: Hao-Ting
authors.initials: HT
authors.lastname: Lu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Weng
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Wang
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000032:analysis; D001710:Biotin / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D001710:Biotin; D004247:DNA; D019809:Streptavidin