Two distinct mechanisms regulate recruitment of murine leukemia virus envelope protein to retroviral assembly sites.
The cytoplasmic tail domain (CTD) of retroviral envelope (Env) proteins has been implicated in modulating Env incorporation into viral particles. We generated a panel of murine leukemia virus (MLV) Env mutants and analyzed their ability to be recruited to human immunodeficiency virus-1 (HIV-1) assembly sites. Surprisingly, the entire CTD was dispensable for recruitment to assembly sites, but a mutation that disrupted the furin cleavage site in Env abolished recruitment. To determine if MLV Env can show selectivity for homologous assembly sites, cells were co-transfected with both HIV-1 and MLV assembly components along with each MLV Env construct and assayed for infectious particle production. MLV Env selectively formed infectious particles with the MLV components at the expense of infectious HIV-1 infectious particle production, but truncation of the CTD progressively reduced this selectivity. Collectively these data suggest that there are two separable mechanisms that govern MLV Env recruitment to viral assembly sites.
Lucas
Tiffany M
TM
Department of Molecular Microbiology and Immunology, Christopher S. Bond Life Science Center, University of Missouri- School of Medicine, Columbia, MO 65211, USA.
Lyddon
Terri D
TD
Grosse
Sarah A
SA
Johnson
Marc C
MC
D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
D054298:Human Immunodeficiency Virus Proteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins