title: HLA class I and class II polymorphisms in Tunisian Berbers.
abstract: BACKGROUND
  The HLA polymorphism is a powerful genetic tool to study population origins. By analysing allele frequencies and haplotypes in different populations, it is possible to identify ethnic groups and establish the genetic relationships among them.
  
  
  OBJECTIVE
  The Berber (endogenous Tunisians) HLA class I and class II genotypes were analysed and compared with those of Mediterranean and Sub-Saharan African communities using genetic distances, Neighbour-Joining dendrograms, correspondence and haplotype analysis.
  
  
  METHODS
  One hundred and five unrelated Berbers were typed for HLA class I (A, B) and class II (DRB1, DQB1) gene alleles using reverse dot-blot hybridization.
  
  
  RESULTS
  High frequencies of A*0201 (24.76%), A*3402 (22.38%) and B*44 (32.85%) alleles were recorded for Berbers, the highest recorded for Mediterranean and North African populations. This study shows a close relatedness of Tunisian Berbers to other Tunisians, North Africans and Iberians.
  
  
  CONCLUSIONS
  The apparent relatedness of Tunisian Berbers to present-day (North African) Tunisians, Algerians and Moroccans suggests that the Arab invasion of North Africa (7(th)-11(th) centuries AD) did not significantly impact the genetic makeup of North Africans. Furthermore, Tunisian Berbers appear to be closely related to Iberians (Spaniards and Basques), indicating that the 7(th) century AD gene flow of invaders was low in Iberians and that the main part of their genetic pool came after the Northward Saharan migration, when hyper-arid conditions were established in Sahara (before 6000 BC). Other studied populations belong to the old Mediterranean substratum, which has been present in the area since pre-Neolithic times. This study indicates a higher proportion of Iberian than Arab ancestry in Tunisian Berbers, which is of value in evaluating the evolutionary history of present-day Tunisians. Greeks seem to share genetic HLA features (Chr 6) with Sub-Saharans. The relatedness of Greeks to Sub-Saharans has been confirmed by other studies based on chromosome 7 genetic markers.
authors.affiliation: National Blood Transfusion Center, Tunis, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Hajjej
authors.forename: M H
authors.initials: MH
authors.lastname: Sellami
authors.forename: H
authors.initials: H
authors.lastname: Kaabi
authors.forename: G
authors.initials: G
authors.lastname: Hajjej
authors.forename: A
authors.initials: A
authors.lastname: El-Gaaied
authors.forename: K
authors.initials: K
authors.lastname: Boukef
authors.forename: W Y
authors.initials: WY
authors.lastname: Almawi
authors.forename: S
authors.initials: S
authors.lastname: Hmida
mesh_terms: D017954:Africa South of the Sahara; D000483:Alleles; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D040961:Genetic Drift; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D019083:Mediterranean Region; D011110:Polymorphism, Genetic; D014416:Tunisia
chemical_list: D005819:Genetic Markers