Involvement of vesicular trafficking system in membrane targeting of the progeny influenza virus genome.
The genome of influenza type A virus consists of single-stranded RNAs of negative polarity. Progeny viral RNA (vRNA) replicated in the nucleus is nuclear-exported, and finally transported to the budding site beneath the plasma membrane. However, the precise process of the membrane targeting of vRNA is unclear, although viral proteins and cytoskeleton are thought to play roles. Here, we have visualized the translocation process of progeny vRNA using fluorescence in situ hybridization method. Our results provide an evidence of the involvement of vesicular trafficking in membrane targeting of progeny vRNA independent of that of viral membrane proteins.
Jo
Shuichi
S
Department of Infection Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Kawaguchi
Atsushi
A
Takizawa
Naoki
N
Morikawa
Yuko
Y
Momose
Fumitaka
F
Nagata
Kyosuke
K
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology*; D004285:Dogs; D016679:Genome, Viral; D017404:In Situ Hybridization, Fluorescence; D009980:Influenza A virus / Q000502:physiology*; D012367:RNA, Viral / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism*; D019065:Virus Assembly
D012367:RNA, Viral