Antioxidant and antiulcerogenic activities of Opuntia ficus indica f. inermis root extract in rats.
Opuntia ficus indica f. inermis methanolic root extract (ORE) was investigated for phenolic and flavonoids contents, in vitro evaluated for DPPH radical scavenging activity, reducing power and in vivo tested for its gastro-protective ability against 80% ethanol induced ulcer in rats. Phytochemical test of ORE were positive for phenolic and flavonoid contents. DPPH radical scavenging activity and reducing power of ORE showed an EC(50) of 118.65±2.51 μg/ml and 300 μg/ml respectively. In vivo the pre-treatment of rats with ranitidine (50 mg/kg) and 200, 400, and 800 mg/kg doses of ORE significantly (p<0.05) reduced the 80% ethanol induced-ulcer lesion, with a rate of 82.68%, 49.21%, 83.13%, and 92.59% respectively, and prevented the depletion of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH), and inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA) in rat stomach tissues when compared with ethanol group. Also pre-treatment with ORE marked a dose-dependent attenuation of histopathology changes induced by ethanol. Phenolic and flavonoids wealth, radical scavenging activity, and reducing power, have been implicated for antiulcer property of ORE.
Alimi
Hichem
H
Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences de Gafsa, Cité Zarroug, 2112 Gafsa, Tunisia.
Hfaiedh
Najla
N
Bouoni
Zouhour
Z
Hfaiedh
Mbarka
M
Sakly
Mohsen
M
Zourgui
Lazhar
L
Rhouma
Khémais Ben
KB
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D029747:Opuntia / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots; D059808:Polyphenols; D011899:Ranitidine; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects* / Q000473:pathology; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000473:pathology
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D008315:Malondialdehyde; D011899:Ranitidine; C004931:1,1-diphenyl-2-picrylhydrazyl; D009195:Peroxidase