title: [The quantitative analysis of Ub and UbE1 in focus of traumatic epilepsy].
abstract: OBJECTIVE
  To study the expression of ubiquitin proteasome system (UPS) in the traumatic epilepsy pathogenesis and its value in traumatic epilepsy by quantitative analysis.
  
  
  METHODS
  Fifteen specimens from human epileptic temporal cortex from PTE were collected as the PTE group. Fifteen specimens from non-PTE were collected as the non-PTE group. Fifteen normal cerebral cortex specimens died from acute traffic accident were collected as the control group. The expression of mRNA and protein of Ub and UbE1 were detected by RT-PCR and Western blot. Statistical analysis was used to compare the data between three groups.
  
  
  RESULTS
  The expression of mRNA and protein of Ub and UbE1 were the following order: PTE group(high), non-PTE group(middle) and control group(low).
  
  
  CONCLUSIONS
  The study confirms that UPS is up-regulated in the epilepsy's focus, especially in traumatic epilepsy. The activation of UPS may be an important pathological change in neurons in pathogenesis of traumatic epilepsy.
authors.affiliation: Department of Forensic Medicine, Zhongshan Medical College, SUN Yat-sen University, Guangzhou 510080, China. seahe7@sina.com.cn
authors.forename: Peng-xiang
authors.initials: PX
authors.lastname: He
authors.forename: Yun-lin
authors.initials: YL
authors.lastname: Tang
authors.forename: Wei-lin
authors.initials: WL
authors.lastname: Cai
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Fang
authors.forename: Hua-lan
authors.initials: HL
authors.lastname: Jing
mesh_terms: D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D006259:Craniocerebral Trauma / Q000150:complications* / Q000473:pathology; D017931:DNA Primers; D004834:Epilepsy, Post-Traumatic / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D049429:Forensic Pathology; D006801:Humans; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; C471538:UBA1 protein, human; D025801:Ubiquitin; D044764:Ubiquitin-Activating Enzymes