title: Molecular characterization of tumors from a transgenic mouse adrenal tumor model: comparison with human pheochromocytoma.
abstract: Adrenal neuroblastoma and pheochromocytoma have the same embryonic origin from neural crest cells and mainly arise from the adrenal medulla. Recently, transgenic mice exhibiting tumors in the bilateral adrenal medulla by the expression of SV40 T-antigen were developed. In this study, we investigated mRNA expression in adrenal tumors of transgenic mice and compared them with human pheochromocytoma by DNA microarray analysis. To compare mouse adrenal tumors and human pheochromacytoma, we found that the expressions of noradrenergic neuron-related genes, including dopa decarboxylase, phenylethanolamine-N-methyltransferase and chromogranin B, were up-regulated in humans but not in mice; however, the expression of neuroblastoma-related genes, including Mycn, paired-like homeobox 2b, gamma-aminobutyric acid A receptor beta3 subunit, islet 1 and kinesin family member 1A, was up-regulated in both species. From the gene expression profiles, the characterization of mouse adrenal tumor, may be similar to that of human adrenal neuroblastoma rather than pheochromacytomas. This mouse model would be a useful tool for the development of anti-cancer drugs and for understanding the etiology of adrenal neuroblastoma.
authors.affiliation: Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan. yhattori@hoshi.ac.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hattori
authors.forename: Naotetsu
authors.initials: N
authors.lastname: Kanamoto
authors.forename: Kumi
authors.initials: K
authors.lastname: Kawano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwakura
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Sone
authors.forename: Masako
authors.initials: M
authors.lastname: Miura
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yasoda
authors.forename: Naohisa
authors.initials: N
authors.lastname: Tamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arai
authors.forename: Takashi
authors.initials: T
authors.lastname: Akamizu
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Maitani
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D010673:Pheochromocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation
chemical_list: D000952:Antigens, Polyomavirus Transforming; D012333:RNA, Messenger