Apigenin inhibits larval growth of Caenorhabditis elegans through DAF-16 activation.
Treatment of Caenorhabditis elegans with apigenin, 5,7,4'-trihydroxyflavone, induces larval growth inhibition. To understand the molecular basis of apigenin-induced larval growth inhibition, the effects of apigenin on DAF-16 activity were examined. DAF-16 was activated through nuclear translocation and the mRNA level of sod-3, one of the known DAF-16 target genes, was increased upon apigenin treatment. DAF-16 activity was required for the growth inhibition, since the larval growth retardation upon apigenin treatment was suppressed in daf-16 mutants. These results indicate that apigenin acts as a stressor that activates DAF-16, which in turn inhibits larval growth.
Kawasaki
Ichiro
I
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Jeong
Myung-Hwan
MH
Oh
Bong-Kyeong
BK
Shim
Yhong-Hee
YH
D021581:Active Transport, Cell Nucleus; D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D051858:Forkhead Transcription Factors; D017238:Genes, Helminth; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; D051858:Forkhead Transcription Factors; D018510:RNA, Helminth; D012333:RNA, Messenger; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; D047310:Apigenin; C525107:Sod-3 protein, C elegans; D013482:Superoxide Dismutase