Comparison of citrated human blood, citrated sheep blood, and defibrinated sheep blood Mueller-Hinton agar preparations for antimicrobial susceptibility testing of Streptococcus pneumoniae isolates.
The use of Mueller-Hinton agar supplemented with citrated human or citrated sheep blood was compared with the use of routinely used Mueller-Hinton agar supplemented with defibrinated sheep blood for antimicrobial susceptibility testing of Streptococcus pneumoniae. The alternate supplements were found to be unsatisfactory, particularly for testing resistant isolates, and therefore are not recommended.
Satzke
Catherine
C
Centre for International Child Health, Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Parkville, Victoria, Australia.
Seduadua
Anna
A
Chandra
Reginald
R
Carapetis
Jonathan R
JR
Mulholland
E Kim
EK
Russell
Fiona M
FM
D000362:Agar; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001769:Blood; D002951:Citrates; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012756:Sheep; D013296:Streptococcus pneumoniae / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D002951:Citrates; D003470:Culture Media; D000362:Agar