Expression of FAM20C in the osteogenesis and odontogenesis of mouse.
Mutations in FAM20C were recently identified as the cause of lethal osteosclerotic bone dysplasia, which highlighted the important role of this molecule in biomineralization. No systematic studies have been performed to evaluate the expression pattern of this relatively new molecule in the developmental processes of bone and tooth. In the present study, we analyzed in detail the expression profile of FAM20C during osteogenesis and odontogenesis using ISH and IHC approaches. The specimens analyzed were mouse tissues spanning embryonic day 13.5 (E13.5) to postnatal 8 weeks. The earliest presence of FAM20C was observed at E14.5. During osteogenesis, FAM20C mRNA was detected in the chondrocytes and osteoblasts of the long bone, whereas its protein was observed in the extracellular matrix (ECM) of bone and in the cytoplasm of the chondrocytes, osteoblasts, and osteocytes. During odontogenesis, FAM20C mRNA was detected in the ameloblasts, odontoblasts, cementoblasts, and periodontal ligament fibroblasts, whereas its protein was observed in the matrices of dentin, enamel, and alveolar bone and in the cytoplasm of the aforementioned cells. The temporospatial expression profile revealed in this study indicates that FAM20C is an ECM protein that may play an important role in controlling the mineralization of bone and tooth.
Wang
Xiaofang
X
Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, TX 75246, USA.
Hao
Jianjun
J
Xie
Yixia
Y
Sun
Yao
Y
Hernandez
Brianda
B
Yamoah
Albert K
AK
Prasad
Monica
M
Zhu
Qinglin
Q
Feng
Jian Q
JQ
Qin
Chunlin
C
D000818:Animals; D000918:Antibody Specificity; D001842:Bone and Bones / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D009805:Odontogenesis / Q000235:genetics*; D010012:Osteogenesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014070:Tooth / Q000254:growth & development / Q000378:metabolism
D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; C509696:FAM20C protein, mouse; D011994:Recombinant Proteins