Transcription factor activator protein-2beta accelerates lipid accumulation in macrophages via enhancing the transcription of CD36.
The role of transcription factor activator protein-2beta (AP-2beta) in foam cell formation was investigated. Uptake assay of DiI-labeled oxidized low density lipoprotein and the lipid quantitative analysis by high performance liquid chromatography showed AP-2beta promoted the lipid accumulation. The quantitative real-time RT-PCR and Western blot indicated that AP-2beta upregulated the expression of CD36. Luciferase assay, electrophoretic mobility shift assay and chromatin immunoprecipitation confirmed that AP-2beta bound to AP-2 binding sites in CD36 promoter region and enhanced the promoter activity of CD36 gene. We conclude that AP-2beta may function in foam cell formation through enhancing the transcription and expression of CD36 followed by increased lipid uptake.
Gan
Lu
L
Department of Radiation Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China. gan_lu@yahoo.com.cn
Zhu
Dongxing
D
Ding
Zhenhua
Z
D000818:Animals; D050197:Atherosclerosis; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018955:CD36 Antigens / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005487:Foam Cells / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D050656:Transcription Factor AP-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D018955:CD36 Antigens; D008077:Lipoproteins, LDL; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D050656:Transcription Factor AP-2; C099252:oxidized low density lipoprotein; D004247:DNA