title: FTIR spectral signature of the effect of cardiotonic steroids with antitumoral properties on a prostate cancer cell line.
abstract: We show in the present work that the infrared (IR) spectrum of human PC-3 prostate cancer cells exposed to anticancer drugs could offer a unique opportunity to get a fingerprint of all the major biochemical components (DNA, RNA, proteins, lipids, etc.) present in the cells and to identify with high sensitivity the signature of the metabolic changes induced by anticancer drugs. We investigated here the FTIR-related signatures of the effect of 4 structurally-related cardiotonic steroids (CS), i.e. ouabain, 19-hydroxy-2″-oxovoruscharin, hellebrin and 19-hydroxy-hellebrin on PC-3 cancer cells incubated between 0 and 36 h in the absence (control) or the presence of the CS. For each molecule a single spectral signature described the largest part of the time dependent modifications with a possible very minor second component. The spectral signatures characterizing the effects of each of the four CS were unique but very similar when compared to the signature of the effect of an intercalating anticancer drug, i.e. doxorubicin, selected as a positive reference compound in our study, suggesting a fully distinct set of cellular perturbations. The current study thus illustrates that Fourier Transform Infrared (FTIR) analyses can be used to identify, among the perturbations induced on a given cancer cell line, the features common to a group of anticancer compounds as well as features specific to every single drug.
authors.affiliation: Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles (ULB), Belgium.
authors.forename: Régis
authors.initials: R
authors.lastname: Gasper
authors.forename: Tatjana
authors.initials: T
authors.lastname: Mijatovic
authors.forename: Audrey
authors.initials: A
authors.lastname: Bénard
authors.forename: Allison
authors.initials: A
authors.lastname: Derenne
authors.forename: Robert
authors.initials: R
authors.lastname: Kiss
authors.forename: Erik
authors.initials: E
authors.lastname: Goormaghtigh
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002018:Bufanolides / Q000737:chemistry / Q000494:pharmacology; D002298:Cardenolides / Q000737:chemistry / Q000494:pharmacology; D002301:Cardiac Glycosides / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D015394:Molecular Structure; D010042:Ouabain / Q000737:chemistry / Q000494:pharmacology; D025341:Principal Component Analysis; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
chemical_list: C497151:2''-oxovoruscharin; D000970:Antineoplastic Agents; D002018:Bufanolides; D002298:Cardenolides; D002301:Cardiac Glycosides; D013844:Thiazoles; D010042:Ouabain; D004317:Doxorubicin; C007913:hellebrin