Structure, chain conformation and antitumor activity of a novel polysaccharide from Lentinus edodes.
A water-soluble polysaccharide LT1 was isolated from the basidiocarps of Lentinus edodes by hot water extraction and ethanol precipitations, further purified by gel chromatography. The Mw of LT1 was estimated to be 642 kDa by using HPGPC. Chemical and spectroscopic studies illustrated that LT1 has a backbone chain composed of 1 → 4-linked and 1 → 3-linked glucopyranosyl residues and has branches of single glucosyl stubs at C-6 of β-(1 → 4)-linked glucopyranosyl. AFM and Congo-red test revealed that LT1 existed as triple helix chain in 0.10 M NaOH solution or distilled water. Our studies showed that LT1 presented significant antitumor bioactivities on Sarcoma180 solid tumor cell implanted in BALB/c mice, which implies that LT1 could be potentially applied as a natural antitumor drug.
Yu
Zhang
Z
Union Hospital of Huazhong University of Science and Technology, 430030 Wuhan, China.
Ming
Gu
G
Kaiping
Wang
W
Zhixiang
Chen
C
Liquan
Dai
D
Jingyu
Liu
L
Fang
Zeng
Z
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D020074:Lentinula / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy*
D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides