MSL cis-spreading from roX gene up-regulates the neighboring genes.
The male-specific lethal (MSL) complex in Drosophila melanogaster paints the male X chromosome in a manner that is both cis and trans to induce 2-fold hypertranscription of the X chromosome. To characterize the upregulation of gene expression by MSL cis-spreading, we measured the expressional change of neighboring genes by microarray when the genes were bound by MSL complexes that spread from an autosomal roX transgene. Genes within a 200kb region that includes roX transgenes were upregulated concurrently with MSL cis-spreading. Conversely, there was almost no expressional change in genes from other regions. RT-PCR and ChIP analyses confirmed that the approximately 2-fold gene hypertranscription was due to MSL cis-spreading. We also demonstrated that upregulation of the neighboring gene could rescue haplo-insufficient phenotypes of the Minute mutant, such as short bristle, delayed adult eclosion and decreased viability. These results indicate that the hypertranscription by MSL cis-spreading is a general mechanism that occurs in several tissue types. Our molecular and genetic data suggest that cis-spreading of the MSL complex from high-affinity sites including the roX gene results in upregulation of the neighboring genes, which are targets for dosage compensation in the male X chromosome.
Park
Seung-Won
SW
Department of Cell Biology and Molecular Medicine, UMDNJ-New Jersey Medical School, 185 South Orange Ave., Newark, NJ 07103, United States.
Oh
Hyangyee
H
Lin
Yuh-Ru
YR
Park
Yongkyu
Y
D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016601:RNA-Binding Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes; D015854:Up-Regulation; D014960:X Chromosome / Q000235:genetics*
D029721:Drosophila Proteins; D009687:Nuclear Proteins; C092595:Pabp2 protein, Drosophila; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C082132:msl-1 protein, Drosophila