Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI.
Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l(-1) Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na(+) ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used. The total production of the homologues was lowered but their spectrum was shifted to higher-molecular-weight compounds.
Rezanka
Tomás
T
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. rezanka@biomed.cas.cz
Prell
Ales
A
Spízek
Jaroslav
J
Sigler
Karel
K
D002087:Butyrates / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D045782:Hemiterpenes; D058610:Isobutyrates; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010421:Pentanoic Acids / Q000032:analysis; D011422:Propionates / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013305:Streptomyces griseus / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D002087:Butyrates; D003470:Culture Media; D045782:Hemiterpenes; D058610:Isobutyrates; D018942:Macrolides; D010421:Pentanoic Acids; D011422:Propionates; C008216:isovaleric acid; C020380:isobutyric acid; C050476:nonactin