title: Biochemical and immunohistochemical characterization of the amyloid in canine amyloid-producing odontogenic tumor.
abstract: The amyloid of canine amyloid-producing odontogenic tumor (APOT) was evaluated biochemically and immunohistochemically. The N-terminal amino-acid sequence of purified amyloid protein from a canine APOT was strikingly similar to the sequence in both rat ameloblastin and porcine sheathlin. Immunohistochemically, the amyloid in APOT from 9 dogs was strongly reactive with anti-rat ameloblastin, anti-porcine sheathlin, and anti-canine APOT amyloid and weakly reactive with anti-porcine amelogenin but negative for antibodies to cytokeratins, vimentin, desmin, alpha-smooth muscle actin, amyloid A, glial fibrillary acidic protein, or S100 protein. The neoplastic epithelial cells of APOT were focally reactive with antibodies to ameloblastin, sheathlin, amelogenin, and canine APOT amyloid. The similarity in amino-acid sequence of the amyloid protein of canine APOT to that of enamel proteins, such as ameloblastin, sheathlin, and amelogenin, and the expression of these antigens in both APOT amyloid and in the neoplastic cells suggest that the amyloid of canine APOT is derived from enamel proteins secreted by ameloblasts.
authors.affiliation: Department of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.forename: T
authors.initials: T
authors.lastname: Miyasho
authors.forename: T
authors.initials: T
authors.lastname: Ohmachi
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Yokota
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008969:Molecular Sequence Data; D009808:Odontogenic Tumors / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D020539:Sequence Analysis, Protein
chemical_list: D000682:Amyloid