title: The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance.
abstract: Farnesol (FOH) is a nonsterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. These isoprenoids inhibit proliferation and induce apoptosis. Here, we show that Aspergillus nidulans AifA encoding the apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase plays a role in the function of the mitochondrial Complex I. Additionally, we demonstrated that ndeA-B and ndiA encode external and internal alternative NADH dehydrogenases, respectively, that have a function in FOH resistance. When exposed to FOH, the ΔaifA and ΔndeA strains have increased ROS production while ΔndeB, ΔndeA ΔndeB, and ΔndiA mutant strains showed the same ROS accumulation than in the absence of FOH. We observed several compensatory mechanisms affecting the differential survival of these mutants to FOH.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café S/N, São Paulo, Brazil.
authors.forename: Taísa Magnani
authors.initials: TM
authors.lastname: Dinamarco
authors.forename: Bárbara de Castro Figueiredo
authors.initials: Bde C
authors.lastname: Pimentel
authors.forename: Marcela
authors.initials: M
authors.lastname: Savoldi
authors.forename: Iran
authors.initials: I
authors.lastname: Malavazi
authors.forename: Frederico Marianetti
authors.initials: FM
authors.lastname: Soriani
authors.forename: Sérgio Akira
authors.initials: SA
authors.lastname: Uyemura
authors.forename: Paula
authors.initials: P
authors.lastname: Ludovico
authors.forename: Maria Helena S
authors.initials: MH
authors.lastname: Goldman
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Goldman
mesh_terms: D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D042967:Electron Transport Complex I / Q000235:genetics / Q000378:metabolism*; D005204:Farnesol / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051033:Apoptosis Inducing Factor; D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D005204:Farnesol; D042967:Electron Transport Complex I