title: Dual candidemia detected by nested polymerase chain reaction in two critically ill children.
abstract: The use of improved microbiological procedures associated with molecular techniques has increased the identification of Candida bloodstream infections, even if the isolation of more than one species by culture methods remains uncommon. We report the cases of two children presenting with severe gastrointestinal disorders and other risk factors that contribute to Candida infections. In the first patient, C. albicans DNA was initially detected by a nested-amplification and C. tropicalis was found later during hospitalization, while blood cultures were persistently negative. In the second child, there was amplification of C. albicans and C. glabrata DNA in the same samples, but blood cultures yielded only C. albicans. Both patients received antifungal therapy but had unfavorable outcomes. These two cases illustrate that PCR was more successful than culture methods in detecting Candida in the bloodstream of high risk children, and was also able to detect the presence of more than one species in the same patient that might impact therapy when the fungi are resistant to azole compounds.
authors.affiliation: Laboratory of Medical Mycology (LIM-53) - Clinical Dermatologic Division, Hospital das Clínicas da FMUSP e Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil. gildamdn@usp.br
authors.forename: G M B
authors.initials: GM
authors.lastname: Del Negro
authors.forename: A F
authors.initials: AF
authors.lastname: Delgado
authors.forename: E R
authors.initials: ER
authors.lastname: Manuli
authors.forename: L
authors.initials: L
authors.lastname: Yamamoto
authors.forename: T S
authors.initials: TS
authors.lastname: Okay
mesh_terms: D002176:Candida albicans / Q000302:isolation & purification; D041221:Candida glabrata / Q000302:isolation & purification; D041022:Candida tropicalis / Q000302:isolation & purification; D058387:Candidemia / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D016638:Critical Illness; D005260:Female; D005767:Gastrointestinal Diseases / Q000150:complications; D006801:Humans; D008297:Male; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*