Use of a mixture of surrogates for infectious bioagents in a standard approach to assessing disinfection of environmental surfaces.
We used a mixture of surrogates (Acinetobacter baumannii, Mycobacterium terrae, hepatitis A virus, and spores of Geobacillus stearothermophilus) for bioagents in a standardized approach to test environmental surface disinfectants. Each carrier containing 10 microl of mixture received 50 microl of a test chemical or saline at 22 +/- 2 degrees C. Disinfectant efficacy criteria were > or = 6 log(10) reduction for the bacteria and the spores and > or = 3 log(10) reduction for the virus. Peracetic acid (1,000 ppm) was effective in 5 min against the two bacteria and the spores but not against the virus. Chlorine dioxide (CD; 500 and 1,000 ppm) and domestic bleach (DB; 2,500, 3,500, and 5,000 ppm) were effective in 5 min, except for sporicidal activity, which needed 20 min of contact with either 1,000 ppm of CD or the two higher concentrations of DB.
Sabbah
Safaa
S
Centre for Research on Environmental Microbiology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5.
Springthorpe
Susan
S
Sattar
Syed A
SA
D040981:Acinetobacter baumannii / Q000187:drug effects*; D017606:Chlorine Compounds / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D004203:Disinfection / Q000379:methods* / Q000592:standards*; D004783:Environmental Microbiology; D001411:Geobacillus stearothermophilus / Q000187:drug effects*; D030041:Hepatitis A virus / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D009170:Nontuberculous Mycobacteria / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology; D010463:Peracetic Acid / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide; D012973:Sodium Hypochlorite; D010463:Peracetic Acid