Characterization and expression of a sea star otx ortholog (Protxβ1/2) in the larva of Patiriella regularis.
A transcript of otx from the sea star Patiriella regularis (Protxβ1/2) was characterized and its expression in early bipinnaria larvae was documented by whole mount in situ hybridization (WMISH). The nucleotide sequence exhibited 94% identity with Amotxβ1/2 from the closely related species Patiria miniata. Protxβ1/2 was expressed strongly in the developing archenteron in the future fore and mid-gut regions. This was followed by expression of Protxβ1/2 in the developing enterocoels, mesodermal derivatives. This suggests a role for Protx in endomesoderm development. In coelom development, Protxβ1/2 was first expressed in the left coelom. Subsequently expression was evident in the right coelom, but localization was never as strong as in the left coelom. This asymmetry in Protxβ1/2 expression in the coeloms was evident up to the stage when they started to extend posteriorly. These data indicate that Protxβ1/2 may have a role in coelom development, particularly in the left coelom, a definitive adult structure.
Elia
Laura
L
School of Medical Sciences, F13, University of Sydney, NSW 2006, Australia.
Cisternas
Paula
P
Byrne
Maria
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005775:Gastrula / Q000196:embryology* / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008648:Mesoderm / Q000196:embryology* / Q000378:metabolism; D008969:Molecular Sequence Data; D051857:Otx Transcription Factors / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013215:Starfish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D051857:Otx Transcription Factors