title: Integrated photoacoustic and fluorescence confocal microscopy.
abstract: We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging of both blood and lymphatic vessels down to the capillary level. Simultaneous photoacoustic angiography and fluorescence lymphangiography were demonstrated, presenting more information to study tumor angiogenesis, vasculature, and microenvironments in vivo.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130-4899, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Konstantin
authors.initials: K
authors.lastname: Maslov
authors.forename: Chulhong
authors.initials: C
authors.lastname: Kim
authors.forename: Song
authors.initials: S
authors.lastname: Hu
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000162:Acoustics; D000792:Angiography / Q000379:methods*; D000818:Animals; D004423:Ear / Q000098:blood supply; D008220:Lymphography / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010777:Photochemistry