title: Evaluation of silymarin as a promising radioprotector.
abstract: Silymarin, a purified extract of seeds of Silybum marianum L. and well known for its hepatoprotective abilities, has been evaluated for inherent utility as a radioprotective agent. A fraction (INM-7035) was authenticated by characterizing the percentage composition of silybin A and B (39.9% and 57.4%). Free radical scavenging activities of INM-7035 against superoxide radicals (>68%), hydroxyl radicals (>33.75%), DPPH (67.2%), and ABTS (32.4%) were also evaluated. The fraction chelated (>30%) ferrous ions, thereby able to restrict amplification. INM-7035 exhibited >50% peroxyl radical scavenging activity in the lipid phase along with dose-dependent (R2 = 0.990) reducing power in the aqueous phase. Radiation-induced free radical flux can lead to disruption of biomolecules like membrane lipids. INM-7035 completely inhibited lipid peroxidative stress in case of membranes against supralethal radiation stress in the liposomal system. The ability of INM-7035 to modulate the levels of NF-kappaB, indicated its inherent potential as a radioprotective bioactive constituent.
authors.affiliation: Institute of Nuclear Medicine and Allied Sciences, Brig SK Mazumdar Marg, Delhi-110054, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Adhikari
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: Raman
authors.initials: R
authors.lastname: Chawla
authors.forename: Jyoti
authors.initials: J
authors.lastname: Sharma
authors.forename: Atlar Singh
authors.initials: AS
authors.lastname: Dhaker
authors.forename: Damodar
authors.initials: D
authors.lastname: Gupta
authors.forename: Neha
authors.initials: N
authors.lastname: Dubey
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Veselin
authors.initials: V
authors.lastname: Ivanov
authors.forename: Veselina
authors.initials: V
authors.lastname: Gadjeva
authors.forename: Renata
authors.initials: R
authors.lastname: Gevrenova
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D001713:Biphenyl Compounds / Q000528:radiation effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003037:Cobalt Radioisotopes; D005419:Flavonoids / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D017930:Genes, Reporter; D029001:Herbal Medicine; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000528:radiation effects; D020944:Silybum marianum / Q000737:chemistry*; D010851:Picrates / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D012838:Silymarin / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001713:Biphenyl Compounds; D003037:Cobalt Radioisotopes; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D011837:Radiation-Protective Agents; D012838:Silymarin; C004931:1,1-diphenyl-2-picrylhydrazyl