Studies of UPLC fingerprint for the identification of Magnoliae officinalis cortex processed.
This study was carried out with the objective of establishing Ultra Performance Liquid Chromatograph (UPLC) fingerprint for the identification of Magnoliae officinalis cortex processed. It was extracted by methanol using an ultrasonic extractor. Twelve samples of M. officinalis cortex produced in Zhejiang of China from different places and species were processed with ginger juice; sample solutions were determined by Waters UPLC equipped with BEH C(18) column and a DAD detector, gradient eluted with formic acid/methanol-formic acid/water as mobile phase. The flow rate was set at 0.3 ml(*) min(-1), while the column temperature was set at 30 degrees C, and the wavelength for detection was set at 240 nm. The characteristic of the common peaks of the UPLC fingerprint for M. officinalis cortex processed are obvious. Forty-one common peaks were detected and two of them were identified. The method of UPLC fingerprint established in this experiment was rapid and efficient. It is an effective means for the quality control of M. officinalis cortex processed.
Wang
Lin
L
Research and Development Center of Natural Medicine, Zhejiang Forestry University, Lin'an 311300, P. R., China.
Yuan
Ke
K
Yu
Wei-Wu
WW