title: Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.
abstract: Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.
authors.affiliation: Research Center for Infectious Diseases, Julius-Maximilians-University Würzburg, D-97080 Würzburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hartmann
authors.forename: Michaela
authors.initials: M
authors.lastname: Dümig
authors.forename: Basem M
authors.initials: BM
authors.lastname: Jaber
authors.forename: Edyta
authors.initials: E
authors.lastname: Szewczyk
authors.forename: Patrick
authors.initials: P
authors.lastname: Olbermann
authors.forename: Joachim
authors.initials: J
authors.lastname: Morschhäuser
authors.forename: Sven
authors.initials: S
authors.lastname: Krappmann
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics*; D009841:Oligonucleotides / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D005819:Genetic Markers; D009841:Oligonucleotides; D004254:DNA Nucleotidyltransferases; C077570:Site-specific recombinase