title: Molecular diversity of Mycobacterium tuberculosis isolates from patients with pulmonary tuberculosis in Mozambique.
abstract: BACKGROUND
  Mozambique is one of the countries with the highest burden of tuberculosis (TB) in Sub-Saharan Africa, and information on the predominant genotypes of Mycobacterium tuberculosis circulating in the country are important to better understand the epidemic. This study determined the predominant strain lineages that cause TB in Mozambique.
  
  
  RESULTS
  A total of 445 M. tuberculosis isolates from seven different provinces of Mozambique were characterized by spoligotyping and resulting profiles were compared with the international spoligotyping database SITVIT2.The four most predominant lineages observed were: the Latin-American Mediterranean (LAM, n = 165 or 37%); the East African-Indian (EAI, n = 132 or 29.7%); an evolutionary recent but yet ill-defined T clade, (n = 52 or 11.6%); and the globally-emerging Beijing clone, (n = 31 or 7%). A high spoligotype diversity was found for the EAI, LAM and T lineages.
  
  
  CONCLUSIONS
  The TB epidemic in Mozambique is caused by a wide diversity of spoligotypes with predominance of LAM, EAI, T and Beijing lineages.
authors.affiliation: Faculty of Veterinary, Eduardo Mondlane University, Maputo Mozambique. viegas_sofia@hotmail.com
authors.forename: Sofia O
authors.initials: SO
authors.lastname: Viegas
authors.forename: Adelina
authors.initials: A
authors.lastname: Machado
authors.forename: Ramona
authors.initials: R
authors.lastname: Groenheit
authors.forename: Solomon
authors.initials: S
authors.lastname: Ghebremichael
authors.forename: Alexandra
authors.initials: A
authors.lastname: Pennhag
authors.forename: Paula S
authors.initials: PS
authors.lastname: Gudo
authors.forename: Zaina
authors.initials: Z
authors.lastname: Cuna
authors.forename: Paolo
authors.initials: P
authors.lastname: Miotto
authors.forename: Véronique
authors.initials: V
authors.lastname: Hill
authors.forename: Tatiana
authors.initials: T
authors.lastname: Marrufo
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Cirillo
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
authors.forename: Gunilla
authors.initials: G
authors.lastname: Källenius
authors.forename: Tuija
authors.initials: T
authors.lastname: Koivula
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009073:Mozambique / Q000453:epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult