title: Source intensity profile in noncontact optical tomography.
abstract: Noncontact optical tomography in reflection mode is often the only possible configuration when imaging the expression of green fluorescent protein (GFP) or other fluorescent proteins in live animals owing to the short penetration depth of visible light. When imaging in reflection mode using noncontact approaches (i.e., without the use of fibers coupled to tissue), correctly accounting for the intensity profile of the source at the surface is a difficult task, usually needing to fit for source positions and/or approximating these to point sources. In this Letter we present a rigorous theoretical approach that directly accounts for the source's intensity profile and verify it using in vivo data from GFP-expressing mice. We show how this approach improves image quality and resolution, while considerably simplifying the forward and inverse problems of the image reconstruction process.
authors.affiliation: Foundation for Research and Technology-Hellas, P.O. Box 1527, Heraklion 71110, Greece.
authors.forename: Ana
authors.initials: A
authors.lastname: Sarasa-Renedo
authors.forename: Rosy
authors.initials: R
authors.lastname: Favicchio
authors.forename: Udo
authors.initials: U
authors.lastname: Birk
authors.forename: Giannis
authors.initials: G
authors.lastname: Zacharakis
authors.forename: Clio
authors.initials: C
authors.lastname: Mamalaki
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000818:Animals; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000032:analysis; D021621:Imaging, Three-Dimensional; D008198:Lymph Nodes / Q000378:metabolism; D051379:Mice; D013499:Surface Properties; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins