The effect of fenugreek on the gene expression of arachidonic acid metabolizing enzymes.
The main bioactive compounds of Trigonella foenum graecum L. (fenugreek) seeds are protodioscin, trigoneoside, diosgenin and yamogenin, which have anticarcinogenic potency through inhibition of cell proliferation and inhibition of prostaglandin synthesis. The effect of fenugreek on ALOX and COX genes was examined in AKR/J H-2(k) mice exposed to dimethylbenz[α]anthracene (DMBA), a potent carcinogen. The expression pattern of these genes was determined by detecting the mRNA expression in various tissues (the lungs, liver, spleen and the kidneys) in four groups of mice. Two groups were fed with normal and two of them with fenugreek containing nutriment. Each group divided into DMBA treated and control groups. Mice were autopsied on day 7 after DMBA treatment for mRNA isolation. Fenugreek consumption itself did not change gene expression compared with the control group. DMBA could increase the expression of ALOX12, ALOX15, ALOX5 genes mainly in all organs. Fenugreek consumption was generally protective in each organ in a different manner. DMBA treatment increased COX2 gene expression, but fenugreek was protective in all tissues examined. In COX1 gene, the fenugreek diet could suppress the expression, except for spleen, independently from carcinogen exposure. Therefore by inhibiting the arachidonic acid metabolism fenugreek may prevent tumorigenesis.
Varjas
Timea
T
University of Pécs, Faculty of Medicine, Institute of Public Health, Pecs, Hungary. vtimi_68@yahoo.com
Nowrasteh
Ghodratollah
G
Budán
Ferenc
F
Horváth
Gábor
G
Cseh
József
J
Gyöngyi
Zoltán
Z
Makai
Sándor
S
Ember
István
I
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001091:Arachidonate Lipoxygenases / Q000187:drug effects / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism; D002273:Carcinogens / Q000633:toxicity; D051545:Cyclooxygenase 1 / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D010936:Plant Extracts / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology; D029910:Trigonella / Q000737:chemistry*
D002273:Carcinogens; D008565:Membrane Proteins; D010936:Plant Extracts; D016718:Arachidonic Acid; D015127:9,10-Dimethyl-1,2-benzanthracene; C056164:fenugreek seed meal; D001091:Arachidonate Lipoxygenases; D051545:Cyclooxygenase 1; C496512:Ptgs1 protein, mouse