title: [Expression of MDR1 and KIT in imatinib-resistant gastrointestinal stromal tumor cells].
abstract: OBJECTIVE
  To explore the relationship between imatinib resistance and genes MDR1 and KIT in gastrointestinal stromal tumor (GIST) cells.
  
  
  METHODS
  The MDR1 and KIT mRNA level in GIST882-R and GIST882-S cells were detected by RT-PCR. Immunocytochemistry and Western blot were employed to detect P-gp and CD117 expression in GIST882-R and GIST882-S cells.
  
  
  RESULTS
  The relative expression of MDR1 mRNA was 0.321 + or - 0.033 in GIST882-R and 0.157 + or - 0.056 in GIST882-S cells, and the difference was statistically significant (P<0.05). The relative expression of KIT mRNA was 0.389 + or - 0.063 in GIST882-R and 0.339 + or - 0.067 in GIST882-S, and the difference was not statistically significant (P>0.05). The relative density of P-gp was 0.443 + or - 0.058 in GIST882-R and 0.237 + or - 0.094 in GIST882-S, and the difference was statistically significant (P<0.05). The relative density of CD117 was 0.744 + or - 0.123 in GIST882-R and 0.704 + or - 0.094 in GIST882-S, and the difference was not statistically significant (P>0.05).
  
  
  CONCLUSIONS
  Over-expression of gene MDR1 may be associated with imatinib resistance in GIST. KIT may not be involved in imatinib resistance.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan Universtity, Shanghai 200032, China.
authors.forename: Jing-lei
authors.initials: JL
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.forename: Li-qing
authors.initials: LQ
authors.lastname: Yao
authors.forename: Ying-yong
authors.initials: YY
authors.lastname: Hou
authors.forename: Yi-hong
authors.initials: YH
authors.lastname: Sun
authors.forename: Kun-tang
authors.initials: KT
authors.lastname: Shen
authors.forename: Ping
authors.initials: P
authors.lastname: Su
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D001549:Benzamides; D015815:Cell Adhesion Molecules / Q000235:genetics; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics*; D006801:Humans; D000068877:Imatinib Mesylate; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D011743:Pyrimidines / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001549:Benzamides; D015815:Cell Adhesion Molecules; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; C054291:antigen 117, Dictyostelium; D000068877:Imatinib Mesylate; D019009:Proto-Oncogene Proteins c-kit