Immobilization of genetically engineered fusion proteins on gold-decorated carbon nanotube hybrid films for the fabrication of biosensor platforms.
We have demonstrated the fabrication of the biosensor platforms by means of the integration of the genetically engineered fusion proteins and the uniform gold nanoparticle-deposited multi-walled nanotube hybrid (Au-MWNT-HB) films for the detection of C-reactive protein (CRP). Au-MWNT-HB films were used as a good electrochemical transducer due to their excellent electrical properties and large surface areas for the signal transduction, while the genetically engineered fusion proteins, or 6His-GBP-SpA fusion proteins, specifically bind onto the surface of the Au-MWNT-HB films and efficiently immobilize bioreceptors for the detection of CRP. As-obtained biosensor platforms were characterized by electrochemical and optical analysis and revealed better performance compared to conventional Au-based biosensors. The concept delineated herein opens a new insight into nanobiotechnology through the integration of genetically engineered biomaterials with carbon nanotube (CNT)-based nanohybrids for emerging applications.
Park
HoSeok
H
Department of Chemical Engineering, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Park
Tae Jung
TJ
Huh
Yun Suk
YS
Choi
Bong Gill
BG
Ko
Sungho
S
Lee
Sang Yup
SY
Hong
Won Hi
WH
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D015202:Protein Engineering
D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006046:Gold