Potential anti-inflammatory diterpenoids from the roots of Caesalpinia mimosoides Lamk.
Anti-inflammatory assay-guided separation of extracts from the roots of Caesalpinia mimosoides Lamk. led to isolation of seven compounds: four diterpenes (1-4), a dimer (9), and two dibenzo[b,d]furans (10, 11) together with eleven known compounds. Their structures were elucidated by 1D- and 2D-NMR techniques as well as UV, IR, mass spectral data and comparison with literature values. The anti-inflammatory activities of all compounds were evaluated for inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW264.7 cell lines. Compounds 4, 6, 8, and 12-14 were also tested for the inhibitory effect on LPS-induced tumor necrosis factor-alpha (TNF-alpha) release in RAW264.7 cells. The results indicated that 4 possessed potent inhibitory activity for both tests with IC(50) values of 3.0 and 6.5 microM, respectively.
Yodsaoue
Orapun
O
Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
Karalai
Chatchanok
C
Ponglimanont
Chanita
C
Tewtrakul
Supinya
S
Chantrapromma
Suchada
S
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029911:Caesalpinia / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D013785:Thailand; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide