title: Development and validation of a hydrophilic interaction chromatography-tandem mass spectrometry for quantification of nicotine and its metabolites in human maternal and cord sera.
abstract: A selective and sensitive HILIC-MS/MS method for the simultaneous quantification of nicotine and its metabolites in human maternal and cord sera was developed and validated. After solid-phase extraction, LC separation was achieved on a hydrophilic interaction chromatography. The validated method is capable of selective identification as well as accurate and sensitive quantification. Analyte recovery ranged from 86.2 to 107.7% and intra- and inter-day assay precision were less than 15% relative standard deviation. This sensitive HILIC-MS/MS method can be used to determine nicotine and its metabolic profile in smokers. This validated method is useful for the determination of nicotine and its metabolites in human serum in future studies of the effects of nicotine exposure on neonatal outcome.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Masato
authors.initials: M
authors.lastname: Goto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Emi
authors.initials: E
authors.lastname: Terayama
authors.forename: Rie
authors.initials: R
authors.lastname: Ito
authors.forename: Koichi
authors.initials: K
authors.lastname: Saito
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sugino
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Makino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009538:Nicotine / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012907:Smoking / Q000097:blood; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009538:Nicotine