title: Antimicrobial activity of the extracts and physalin D from Physalis alkekengi and evaluation of antioxidant potential of physalin D.
abstract: Physalis alkekengi L. (Solanaceae) is a popular plant in traditional European and Chinese folk medicine, and it has been reported to have many ethnopharmacological properties including antifungal, anti-cough, anti-inflammatory, analgesic, and febricide activities. Some active components from Physalis species have been investigated. However, no antimicrobial activity studies on extracts and physalins of P. alkekengi have been carried out. In this study, we attempted to identify the possible antimicrobial activities of the methanol extract from aerial parts of P. alkekengi and the dichloromethane extract from calyces of the plant. The extracts were tested against five Gram-positive and five Gram-negative bacteria and five Candida species by using disk diffusion and broth microdilution methods. The extracts were fractionated to isolate physalins using chromatographic techniques, and physalin D was isolated from the extracts. The structure of the compound was elucidated on the basis of (1)H-NMR spectroscopic study, and confirmed by comparison with a reference sample and literature data. Results indicated that all the extracts and physalin D were characterized by antibacterial action, especially against Gram-positive bacteria, with MIC values between 32 and 128 microg/mL. The methanol extract had moderate activity against fungi at MICs ranging from 128 to 512 microg/mL, but the dichloromethane extract and physalin D had low activity against fungi at MICs ranging from 256 to 512 microg/mL. Additionally, the antioxidant activity of physalin D was evaluated by qualitative DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and TBA (thiobarbituric acid) assays. Physalin D showed low antioxidant activity with an IC(50) value of >or= 10 +/- 2.1.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenisehir Campus, Mersin, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Helvaci
authors.forename: G
authors.initials: G
authors.lastname: Kökdil
authors.forename: M
authors.initials: M
authors.lastname: Kawai
authors.forename: N
authors.initials: N
authors.lastname: Duran
authors.forename: G
authors.initials: G
authors.lastname: Duran
authors.forename: A
authors.initials: A
authors.lastname: Güvenç
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D002175:Candida / Q000187:drug effects; D002460:Cell Line; D035264:Flowers / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D008826:Microbial Sensitivity Tests; D032321:Physalis / Q000737:chemistry*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012632:Secosteroids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D012632:Secosteroids; D017392:Thiobarbituric Acid Reactive Substances; C441429:physalin D; C004931:1,1-diphenyl-2-picrylhydrazyl