title: Basic characterization of 90 kDa heat shock protein genes HSP90AA1, HSP90AB1, HSP90B1 and TRAP1 expressed in Japanese quail (Coturnix japonica).
abstract: In the current study, we describe four novel members of the 90 kDa heat shock protein (HSP90) family expressed in Japanese quail, Coturnix japonica. The coding regions of the genes, CjHSP90AA1, CjHSP90AB1, CjHSP90B1 and CjTRAP1, exhibited more than 94% similarity to their related genes in chicken. The putative proteins encoded by these quail genes contained motifs considered essential for HSP90 gene function. In addition, the predicted proteins were more similar to HSP90AA1, HSP90AB1, HSP90B1 and TRAP1 proteins expressed in vertebrates than they were to other members of the HSP90 family. Exon numbers of CjHSP90AA1 (11), CjHSP90AB1 (12) or CjTRAP1 (18) are the same as the chicken and mammalian orthologs. Furthermore, gene order in the regions surrounding CjHSP90AB1 and CjTRAP1 has been preserved, providing evidence that the genomic regions were orthologous to HSP90-containing regions in the chicken genome. The promoter regions of the genes also contained conserved motifs identified in related genes of chicken. However, the nucleotide sequences of the 5'-flanking region of these genes were highly polymorphic. We also found that CjHSP90AA1 exhibited a robust response to heat shock treatment. Taken together, the data suggest that CjHSP90AA1, CjHSP90AB1, CjHSP90B1 and CjTRAP1 encode orthologs of HSP90AA1, HSP90AB1, HSP90B1 and TRAP1, respectively.
authors.affiliation: Department of Animal Science, Tokyo University of Agriculture, Atsugi, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Nagahori
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Maruyama
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hosomichi
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hara
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kei
authors.initials: K
authors.lastname: Hanzawa
mesh_terms: D000818:Animals; D003370:Coturnix / Q000235:genetics*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*
chemical_list: D018841:HSP90 Heat-Shock Proteins