Agar-based disk diffusion assay for susceptibility testing of dermatophytes.
Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is simple, and could be used to determine the antifungal susceptibility of dermatophytes.
Nweze
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Center for Medical Mycology, University Hospitals Case Medical Center and Case Western Reserve University, Cleveland, OH 44106, USA.
Mukherjee
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Ghannoum
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MA
D000362:Agar; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000302:isolation & purification; D003470:Culture Media / Q000737:chemistry; D004058:Diffusion; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D015203:Reproducibility of Results
D000935:Antifungal Agents; D003470:Culture Media; D000362:Agar