Essential oil compositions and antioxidant properties of the roots of twelve Anatolian Paeonia taxa with special reference to chromosome counts.
Essential oil compositions and antioxidant potentials of fourteen ethanol (75%) root extracts prepared from twelve taxa of the genus Paeonia (Paeoniaceae), including P. arietina Anders., P. daurica Andrews, P. xkayae N. Ozhatay, P. kesrouanensis Thiéb., P. mascula (L.) Miller subsp. arasicola G. Kaynak, ö. Yilmaz & R. Daşkin, P. mascula (L.) Miller subsp. bodurii N. Ozhatay, P. cf. mascula L. (Mill.) subsp. mascula (two samples from central and northeastern Anatolia), P. cf. officinalis Retz., P. peregrina Miller (two samples from western and northwestern Anatolia), P. tenuifolia L., P. turcica Davis & Cullen, and P. wittmanniana Hartwiss ex Lindl. were assessed. The chromosome numbers of the root tips of the species were examined using chromosome staining technique with Shiff's reagent under Leitz microscope. The essential oils of the roots of the Paeonia species were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and the major components were identified as salicylaldehyde (10%-94.4%), cis-myrtanal (5.5%-59.7%), and methyl salicylate (2%-52.2%). Antioxidant potentials were tested against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radicals using propyl gallate and rutin as the references. Total phenolic contents of the ethanol extracts were determined using Folin-Ciocalteau's method. The extracts exerted moderate NO scavenger effect and displayed insignificant DPPH radical scavenger activity at 500 microg mL(-1). On the other hand, P. daurica, P. tenuifolia and P. cf. mascula subsp. mascula are diploids with 2n = 10, while other nine taxa are tetraploids with 2n = 20.
Orhan
Ilkay
I
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey. iorhan@gazi.edu.tr
Demirci
Betül
B
Omar
Iman
I
Siddiqui
Huma
H
Kaya
Erdal
E
Choudhary
M Iqbal
MI
Ecevit-Genç
Gülay
G
Ozhatay
Neriman
N
Sener
Bilge
B
Başer
K Hüsnü Can
KH
D000975:Antioxidants / Q000302:isolation & purification*; D032461:Chromosomes, Plant / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification; D009822:Oils, Volatile / Q000302:isolation & purification*; D029596:Paeonia / Q000145:classification* / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile