title: [A verification study on the genes associated with laryngeal squamous cell carcinoma by cDNA microarray].
abstract: OBJECTIVE
  Our previous study revealed many genes differentially expressed in human laryngeal squamous cell carcinoma tissues (LSCC) and related adjacent normal tissues. We verificated the differentiated expressions of target genes, possibly related to LSCC. And these results can be foundations of further study of these target genes function.
  
  
  METHODS
  SENP1, CD109, Laminin alpha 2, Laminin alpha 3 were selected according to the cDNA microarray results. The expression of these genes mRNA and protein were detected by RT-PCR and Western blot in 12 cases of LSCC and related adjacent normal tissues.
  
  
  RESULTS
  The mRNA expression of SENP1, CD109 and Laminin alpha 3 were significantly higher while Laminin alpha 2 were significantly lower in LSCC tissues than in corresponding adjacent normal tissues by Semiquantitative RT-PCR. Western blot analysis revealed SENP1, CD109 protein expression were significantly higher in LSCC tissues than in corresponding adjacent normal tissues.
  
  
  CONCLUSIONS
  SENP1, CD109, Laminin alpha 2 and Laminin alpha 3 may correlated with tumorigenesis and development of LSCC and can provide beneficial clue for study pathogenesis of LSCC in molecular level.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, 210008, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Shen
authors.forename: Rongsheng
authors.initials: R
authors.lastname: Ni
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Qian
authors.forename: Chenjie
authors.initials: C
authors.lastname: Yu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Ni
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhu
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000235:genetics; D058851:GPI-Linked Proteins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D015703:Antigens, CD; C120523:CD109 protein, human; D058851:GPI-Linked Proteins; D007797:Laminin; D009363:Neoplasm Proteins; C102645:laminin alpha 2; C095567:laminin alpha 3; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases