title: Structural basis for the decrease in the outward potassium channel current induced by lanthanum.
abstract: The current of the outward K+ channel in the cell of horseradish treated with La3+ and the direct interaction between La3+ and the K+ channel protein were investigated using the whole-cell patch-clamp technique, molecular dynamics simulation, and quantum chemistry calculation methods. It was found for the first time that La3+ decreases the current of the K+ channel in the horseradish mesophyll cell. The decrease results from the formation of a coordination bond and hydrogen bond between La3+ and the K+ channel protein in the plasma membrane. The direct interaction destroys the native structure of the K+ channel protein, disturbing the function of the K+ channel protein in the cells. The results can provide the theoretical foundation for understanding the interaction between metal ions (especially high-valence metal ions) and the channel protein in organisms, including animal and plant cells.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210046, People's Republic of China.
authors.forename: Li Hong
authors.initials: LH
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Jiang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Li
authors.forename: Tian Hong
authors.initials: TH
authors.lastname: Lu
authors.forename: Xiao Lan
authors.initials: XL
authors.lastname: Ding
authors.forename: Xiao Hua
authors.initials: XH
authors.lastname: Huang
mesh_terms: D000818:Animals; D031215:Armoracia / Q000166:cytology; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D007811:Lanthanum / Q000378:metabolism*; D058503:Mesophyll Cells / Q000166:cytology / Q000378:metabolism; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D011789:Quantum Theory
chemical_list: D010940:Plant Proteins; D015221:Potassium Channels; D007811:Lanthanum; D011188:Potassium