Antioxidant activity of oligosaccharide ester extracted from Polygala tenuifolia roots in senescence-accelerated mice.
The constituents of the ethanol extract from the root of Polygala tenuifolia Willd. (Polygalaceae) were investigated for antioxidant activity in senescence-accelerated mice. Consequently, two relevant samples were obtained, a fraction separated by macroporous resin (YZ-OE), and a major pure crystal of 3,6'-disinapoyl sucrose (DISS). Based on HPLC-ESI-MS analysis, the most constituents in the YZ-OE fraction from the extract of P. tenuifolia were oligosaccharide esters. The antioxidant activities of these two samples were evaluated using the accelerated senescence-prone, short-lived mice (SAMP) in vivo. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were increased significantly in SAMP mice fed oligosaccharide esters (YZ-OE 50 mg/kg) and its constituents (DISS 50 mg/kg). However, the content of malondialdehyde (MDA) was increased in the blood and liver of SAMP mice. But when given YZ-OE, it could be decreased, by 44.3% and 47.5%, respectively, compared with the SAMP model. Results from the analyses indicated that the oligosaccharide esters (YZ-OE) from roots of P. tenuifolia had a high in vivo antioxidant activity.
Liu
Ping
P
Department of Clinical Pharmacology, Pharmacy Care Center, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Hu
Yuan
Y
Guo
Dai-Hong
DH
Lu
Bao-Rong
BR
Rahman
Khalid
K
Mu
Li-Hua
LH
Wang
Dong-Xiao
DX
D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D004952:Esters; D005979:Glutathione Peroxidase / Q000097:blood; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D009844:Oligosaccharides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D010936:Plant Extracts / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots; D031801:Polygala; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D004952:Esters; D009844:Oligosaccharides; D010936:Plant Extracts; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase