Thermophilic bacteria from Mexican thermal environments: isolation and potential applications.
Extremophiles are microorganisms that possess application possibilities in several industrial fields, including agricultural, chemical, laundry, pharmaceutical, food, petroleum and bioremediation. This work reports the isolation of 19 thermophilic, alkalitolerant and halotolerant bacterial strains from two thermal sites in Veracruz, México: El Carrizal thermal pool and Los Baños hot spring. These strains belong to the Geobacillus, Anoxybacillus and Aeribacillus genera. The strains produce lipases, proteases, and amylases under thermophilic conditions. They may have good potential for application in microbial enhanced oil recovery, since they are thermophilic and halotolerant, produce exopolymers (up to 11.8 mg/mg) and acids, show emulsifying activity (E24 up to 7.5%), and are able to grow in kerosene as carbon source; these strains may also be used in biodesulphurization since they can grow in dibenzothiophene producing 2-hydroxybiphenyl under thermophilic conditions (up to 2.9 mg/L).
Pinzón-Martínez
D L
DL
Unidad de Investigación y Desarrollo en Alimentos, Instituto Tecnológico de Veracruz, Veracruz, México, Av. Miguel A. de Quevedo 2779, Veracruz, Ver 91897, México.
Rodríguez-Gómez
C
C
Miñana-Galbis
D
D
Carrillo-Chávez
J A
JA
Valerio-Alfaro
G
G
Oliart-Ros
R
R
D000681:Amylases / Q000378:metabolism; D001406:Bacillaceae / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology*; D008049:Lipase / Q000378:metabolism; D008800:Mexico; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases