Cell-free expression and labeling strategies for a new decade in solid-state NMR.
Although solid-state NMR and cell-free expression have recently become standard methods in biology, the combination of the two is still at a very early stage of development. In this article, we will explore several approaches by which cell-free expression could help solid-state NMR in its quest for biomolecular structure and mechanism elucidation. Far from being just another structure determination technique, this quest is motivated by the unique possibility of using solid-state NMR to determine the high resolution structure of a membrane protein within its native environment, the lipid membrane. We will examine the specific sample preparation requirements that such a goal imposes and how cell-free expression can play a key role in such a protocol.
Abdine
Alaa
A
UMR 7099, CNRS and Université Paris Diderot, IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris, France.
Verhoeven
Michiel A
MA
Warschawski
Dror E
DE
D002474:Cell-Free System; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017433:Protein Structure, Secondary
D008051:Lipid Bilayers; D008565:Membrane Proteins