Complete genome sequence and recombination analysis of infectious bronchitis virus attenuated vaccine strain H120.
The strain H120 of infectious bronchitis virus (IBV) is one of the earliest and representative attenuated live Infectious Bronchitis vaccine strains. To investigate the genomic feature of H120 and further understand its role in the epidemiology of IBV, complete genome of H120 was sequenced and compared with sequences of other IBV strains by phylogenetic and recombination analysis. The complete genome of H120 is 27631 nucleotides in length and has a similar structure with that of Beaudette strain. We found that strain ZJ971 is probably a virulence revertant of H120. Nine amino acids changes and a three-nucleotide deletion were identified in ZJ971. Besides, potential recombination events associated with H120 were found in five IBV strains including H52, KQ6, SAIBK, Ark DPI 11, and Ark DPI 101. This study suggested that H120 might have contributed to the emergence of new IBV variants through both virulence reversion and recombination.
Zhang
Yi
Y
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, School of Life Science, Sichuan University, #29 Wangjiang Road, Chengdu, Sichuan Province, China.
Wang
Hong-Ning
HN
Wang
Ting
T
Fan
Wen-Qiao
WQ
Zhang
An-Yun
AY
Wei
Kun
K
Tian
Guo-Bao
GB
Yang
Xin
X
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D018352:Coronavirus Infections / Q000517:prevention & control / Q000662:veterinary*; D016679:Genome, Viral; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011201:Poultry Diseases / Q000517:prevention & control / Q000821:virology*; D011995:Recombination, Genetic; D014613:Vaccines, Attenuated / Q000145:classification / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics*; D014774:Virulence
D014613:Vaccines, Attenuated; D014765:Viral Vaccines