Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analysis.
In this study, the concept of size-selective proteolysis was first described by using the mesoporous silica-based trypsin nanoreactor. For analysis of a complex protein sample, low-MW proteins were preferentially digested for identification while high-MW proteins were excluded from digestion.
Min
Qianhao
Q
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Wu
Ren'an
R
Zhao
Liang
L
Qin
Hongqiang
H
Ye
Mingliang
M
Zhu
Jun-Jie
JJ
Zou
Hanfa
H
D004800:Enzymes, Immobilized / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties; D014357:Trypsin / Q000737:chemistry*
D004800:Enzymes, Immobilized; D020543:Proteome; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D014357:Trypsin