Effect of leukoreduction on transfusion-induced inflammation in dogs.
BACKGROUND
Removal of leukocytes (LR) has been shown to eliminate or attenuate many of the adverse effects of transfusion in experimental animals and humans.


OBJECTIVE
Transfusion of stored packed red blood cells (pRBCs) is associated with an inflammatory response in dogs and prestorage LR attenuates the inflammatory response.


METHODS
Thirteen random-source, clinically healthy, medium and large breed dogs.


METHODS
Experimental study. On day 0, animals were examined and baseline blood samples were collected for analysis. Whole blood was then collected for processing with and without LR, and stored as pRBC. Twenty-one days later, stored pRBCs were transfused back to the donor. Blood samples were collected before and 1 and 3 days after transfusion.


RESULTS
In the dogs that received non-LR pRBCs (n = 6) there was a significant increase from baseline in white blood cell count from a mean (SD) of 8.20 (2.74) to 13.95 (4.60) × 10(3) cells/μL (P < .001) and in segmented neutrophil count from a mean (SD) of 5.76 (2.70) to 11.91 (4.71) × 10(3) cells/μL (P < .001). There were also significant increases in fibrinogen from a mean (SD) of 129.7 (24.2) to 268.6 (46.7) mg/dL (P < .001) and C-reactive protein from a mean (SD) of 1.9 (2.1) to 78.3 (39.3) μg/mL (P < .001). There was no significant increase from baseline in any of the markers in the dogs that received LR pRBC (n = 5).


CONCLUSIONS
There is a profound inflammatory response to transfusion in normal dogs, which is eliminated by LR of the pRBC units.
McMichael
M A
MA
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois, Urbana, IL, USA. mmcm@illinois.edu
Smith
S A
SA
Galligan
A
A
Swanson
K S
KS
Fan
T M
TM
D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D004283:Dog Diseases / Q000209:etiology*; D004285:Dogs; D017707:Erythrocyte Transfusion / Q000009:adverse effects / Q000662:veterinary*; D005260:Female; D007249:Inflammation / Q000209:etiology / Q000517:prevention & control / Q000662:veterinary*; D047589:Leukocyte Reduction Procedures / Q000662:veterinary*; D008297:Male