title: Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination.
abstract: Schizophyllum commune is the only mushroom-forming fungus in which targeted gene deletions by homologous recombination have been reported. However, these deletions occur with a low frequency. To overcome this, the ku80 gene of S. commune was deleted. This gene is involved in the nonhomologous end-joining system for DNA repair. The Deltaku80 strain was not affected in growth and development. However, the transformation efficiency was reduced up to 100-fold. This was accompanied by a strong increase in the relative number of transformants with a homologous integration of a knockout construct. Genes sc15, jmj3 and pri2 were deleted in the Deltaku80 strain. In total, seven out of 10 transformants showed a gene deletion. This frequency will facilitate a systematic analysis of gene function in S. commune.
authors.affiliation: Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, Utrecht, the Netherlands.
authors.forename: Jan F
authors.initials: JF
authors.lastname: de Jong
authors.forename: Robin A
authors.initials: RA
authors.lastname: Ohm
authors.forename: Charissa
authors.initials: C
authors.lastname: de Bekker
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
authors.forename: Luis G
authors.initials: LG
authors.lastname: Lugones
mesh_terms: D034961:Antigens, Nuclear / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D055786:Gene Knockout Techniques; D005827:Genetics, Microbial / Q000379:methods; D000072200:Ku Autoantigen; D011995:Recombination, Genetic; D012567:Schizophyllum / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D000072200:Ku Autoantigen