Isolation of Photobacterium sp. LuB-1 and its application in rapid assays for chemical toxicants in water.
OBJECTIVE
To isolate marine bacteria with strong bioluminescence in a wide range of NaCl concentration, especially at low salt conditions.


RESULTS
A luminous bacterium named LuB-1 was isolated from China. It was identified by biochemical analysis and phylogenetic analysis based on the 16S rRNA gene and designated as Photobacterium sp. The isolate is capable of emitting strong and stable luminescence in a wide range of NaCl concentration from 0.2 to 5% (w/v). For most toxic agents tested in this study, the response of LuB-1 was better than that of MicrotoxVibrio fischeri under both low salt (0.9% NaCl) and high salt (2.0% NaCl) conditions.


CONCLUSIONS
The strain LuB-1 had an obvious predominance of bioluminescence in a wide range of NaCl concentration and better response for heavy metal pollutants and some organic toxicants in both low and high salt toxicity test systems.


CONCLUSIONS
Because of its good sensitivity in a wide range of salt concentration, the strain LuB-1 should have its unique advantage in rapid assay for toxicants in water with different salt concentrations.
Hong
Y
Y
Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Chen
Z
Z
Zhang
B
B
Zhai
Q
Q
D015373:Bacterial Typing Techniques; D001681:Biological Assay / Q000379:methods*; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D049449:Luminescence; D019216:Metals, Heavy / Q000032:analysis* / Q000633:toxicity*; D008969:Molecular Sequence Data; D010776:Photobacterium / Q000187:drug effects* / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D012965:Sodium Chloride