Nuclear morphometry in prognostication of breast cancer in Saudi Arabian patients: comparison with European and African breast cancer.
BACKGROUND
The role of nuclear morphometry as a prognostic factor in breast cancer is well documented. The aim of this study was to evaluate this role in breast cancer in Saudi patients and to compare it with the experience in some African and European studies.


METHODS
Primary tumors from 135 patients were analyzed using an image overlay drawing system (Prodit Morphometry Program), for the following nuclear features: area, perimeter, diameter, and roundness.


RESULTS
The mean nuclear area (NA) was 93 microm(2) (range 45-168 microm(2)). The values of NA were higher in lymph node-positive patients than lymph node-negative patients and in advanced stages than early cancer. NA was significantly larger in patients with high grade tumor (p<0.0001) and in cases with tumor invasion (p<0.01). NA also was significantly larger in recurrent cases (103 microm(2)) than in non-recurrent ones (91 microm(2)). In univariate (Kaplan-Meier) analysis, NA was a significant predictor of disease-free survival (DFS) (log rank p<0.01), but not disease-specific survival (DSS). In multivariate (Cox) survival analysis, NA lost its significance as an independent predictor; response to treatment (p=0.0001) and tumor grade (p=0.030) being the only predictors of DFS. In a similar analysis for DSS, recurrence (p=0.040) and stage (p=0.003) were the only independent predictors.


CONCLUSIONS
Nuclear morphometric profiles are helpful in identifying aggressive tumor phenotype (i.e. cases at risk for recurrence). The cut-off (93 mum(2)) of NA might be applied as quantitative criterion for Saudi female breast cancer to separate patients into good and poor prognosis groups. Mean NA of Saudi patients was markedly higher than the reported mean NA in the other studies and these differences might be due to technical variations or genetic bases.
Buhmeida
Abdelbaset
A
Center of Excellence in Genomic Medicine Research (CEGMR). King Abdul-Aziz University. P.O. Box: 80216 Jeddah 21589, Saudi Arabia. abuhmeida@kau.edu.sa
Al-Maghrabi
Jaudah
J
Merdad
Adnan
A
Al-Thubaity
Fatima
F
Chaudhary
Adeel
A
Gari
Mamdooh
M
Abuzenadah
Adel
A
Collan
Yrjö
Y
Syrjänen
Kari
K
Al-Qahtani
Mohammed
M
D000328:Adult; D000349:Africa; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000401:mortality* / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology*; D005060:Europe; D005260:Female; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D016707:Tissue Fixation