title: Wild mushrooms Clitocybe alexandri and Lepista inversa: in vitro antioxidant activity and growth inhibition of human tumour cell lines.
abstract: The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract of L. inversa was the most potent as antioxidant (EC(50)<1.8 ± 0.1mg/ml), while the phenolic ethanolic extract of C. alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (GI(50)<26.0 ± 1.3 μg/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds.
authors.affiliation: Mountain Research Centre CIMO, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
authors.forename: Josiana A
authors.initials: JA
authors.lastname: Vaz
authors.forename: Sandrina A
authors.initials: SA
authors.lastname: Heleno
authors.forename: Anabela
authors.initials: A
authors.lastname: Martins
authors.forename: Gabriela M
authors.initials: GM
authors.lastname: Almeida
authors.forename: M Helena
authors.initials: MH
authors.lastname: Vasconcelos
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis; D019163:Reducing Agents / Q000737:chemistry; D012015:Reference Standards; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D011134:Polysaccharides; D019163:Reducing Agents; D019207:beta Carotene; C004931:1,1-diphenyl-2-picrylhydrazyl