title: Molecular cloning and expression of cytochrome P450 2D6 in the livers of domestic cats.
abstract: Cytochrome P450 2D (CYP2D) is one of the major metabolizing enzymes for drugs in humans and other mammals. Although domestic cats (Felis catus) are the most common companion animal in many countries, the properties of their drug-metabolizing system are largely unknown. We determined the complete cDNA sequence of the CYP2D open reading frame (1,500 bp) with both 5'- and 3'-untranslated regions in domestic cats. Feline CYP2D consists of 500 deduced amino acids and shows high homology with CYP2Ds of primates and rodents, particularly with the canine CYP2D homologue, and was designated as CYP2D6 based on its genomic structure. CYP2D6 transcripts were expressed predominantly in the liver and to a much lesser in the testis, suggesting the role of xenobiotic metabolism in the liver.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Komatsu
authors.forename: Kouichi
authors.initials: K
authors.lastname: Honda
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Takio
authors.initials: T
authors.lastname: Kitazawa
authors.forename: Takeo
authors.initials: T
authors.lastname: Hiraga
authors.forename: Hiroki
authors.initials: H
authors.lastname: Teraoka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats / Q000378:metabolism*; D003001:Cloning, Molecular; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D018076:DNA, Complementary; D019389:Cytochrome P-450 CYP2D6