title: Characterization of the metabolic phenotype of chronically activated lymphocytes.
abstract: Activated lymphocytes proliferate, secrete cytokines, and can make antibodies. Normally activated B and T cells meet the bioenergetic demand for these processes by up-regulating aerobic glycolysis. In contrast, several lines of evidence suggest that pathogenic lymphocytes in autoimmune diseases like lupus meet ATP demands through oxidative phosphorylation. Using (13)C-glucose as a stable tracer, we found that splenocytes from mice with lupus derive the same fraction of lactate from glucose as control animals, suggesting comparable levels of glycolysis and non-oxidative ATP production. However, lupus splenocytes increase glucose oxidation by 40% over healthy control animals. The ratio between pentose phosphate cycle (PPC) activity and glycolysis is the same for each group, indicating that increased glucose oxidation is due to increased activity of the TCA cycle in lupus splenocytes. Repetitive stimulation of cultured human T cells was used to model chronic lymphocyte activation, a phenotype associated with lupus. Chronically activated T cells rely primarily on oxidative metabolism for ATP synthesis suggesting that chronic antigen stimulation may be the basis for the metabolic findings observed in lupus mice. Identification of disease-related bioenergetic phenotypes should contribute to new diagnostic and therapeutic strategies for immune diseases.
authors.affiliation: Chemical Biology Doctoral Program, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Wahl
authors.forename: B
authors.initials: B
authors.lastname: Petersen
authors.forename: R
authors.initials: R
authors.lastname: Warner
authors.forename: B C
authors.initials: BC
authors.lastname: Richardson
authors.forename: G D
authors.initials: GD
authors.lastname: Glick
authors.forename: A W
authors.initials: AW
authors.lastname: Opipari
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB; D010084:Oxidation-Reduction; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000941:Antigens; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose