Accumulation of selenium and changes in the activity of inulinase and catalase in the cells of Kluyveromyces marxianus on pulsed electric field treatment.
Pulsed electric field (PEF) of 1Hz, 1.5 kV, and 1 ms increased the activities of catalase and inulinase over the whole range of applied Se concentrations compared with the non-treated cultures. A significant effect of selenium concentration (in the range of 5-14 microg/ml) on both intra- and extracellular enzyme activities was noted. At a Se concentration of 10 microg/ml, the activities of intra- and extracellular inulinases and extracellular catalase in the PEF-treated cultures reached the maximum of 71 U/g d.m., 46 U/g d.m., and approx. 8 U/ml, respectively. The maximum activity of intracellular catalase of approx. 6 U/ ml (with and without PEF) was recorded at 5 microg Se/ml. Further increasing of selenium concentration caused a decrease in the activity of the enzymes.
Pankiewicz
Urszula
U
Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, Lublin, Poland. urszula.pankiewicz@up.lublin.pl
Jamroz
Jerzy
J
D002374:Catalase / Q000032:analysis / Q000378:metabolism*; D018274:Electroporation; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007716:Kluyveromyces / Q000201:enzymology / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*
D002374:Catalase; D006026:Glycoside Hydrolases; C023086:inulinase; D012643:Selenium