title: Safety test and field study of an inactivated oil-adjuvanted H5N1 avian influenza vaccine.
abstract: We previously reported the development of an inactivated oil-adjuvanted avian influenza vaccine using an apathogenic H5N1 strain of the same lineage as the Eurasian lineage viruses currently epidemic in Asia. In this study, we confirmed the safety and evaluated the efficacy of this vaccine in layer chicken farms by field trials. No problematic adverse reactions occurred in the safety test. In addition, no adverse effects were observed in the field trial, and the antibody titer exceeded a protective level (hemagglutination inhibition (HI) antibody titer of 16) at 3 weeks after a single injection. Based on the above findings, this vaccine was confirmed to be safe and induced a protective level of antibody titer with a single injection in the chickens at the farms.
authors.affiliation: Division 2, Second Research Department, The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan. imamura-taka@kaketsuken.or.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Imamura
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Sakamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Sasaki
authors.forename: Norihide
authors.initials: N
authors.lastname: Kokumai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ohgitani
authors.forename: Akira
authors.initials: A
authors.lastname: Sawata
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Lin
authors.forename: Masashi
authors.initials: M
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D002642:Chick Embryo / Q000821:virology; D002645:Chickens; D004372:Ducks / Q000276:immunology / Q000821:virology; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007115:Immunization Schedule; D053124:Influenza A Virus, H5N1 Subtype / Q000254:growth & development / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*; D005585:Influenza in Birds / Q000453:epidemiology / Q000276:immunology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D008380:Marek Disease / Q000276:immunology; D012449:Safety
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases