[Expressions of D2-40 and CD34 in invasive ductal carcinoma of the breast and the clinical implications].
OBJECTIVE
To investigate the expression of D2-40 and CD34 in invasive ductal carcinoma of the breast and the clinical significance.


METHODS
D2-40 and CD34 expressions were detected immunohistochemically in 108 cases of invasive ductal carcinoma of the breast and 30 cases of breast fibroadenoma. The lymphatic microvessel density (LMD) and the microvessel density (MD), marked by D2-40 and CD34, respectively, were calculated and their relationship with the clinicopathological factors was analyzed.


RESULTS
The LMD and MD for invasive ductal carcinoma of the breast were significantly higher than those of breast fibroadenoma (P<0.01). One-way ANOVA indicated that the LMD differed significantly between tumors of different histological grades, status of lymph node metastasis and TNM stages; the MD also varied significantly with the tumor size, histological grade, lymph node metastasis and TNM stage. The LMD and MD showed a positive correlation to the tumor size (r=0.335, 0.337), histological grade (r=0.580, 0.671), lymph node metastasis (r=0.690, 0.721) and TNM stage (r=0.623, 0.634), but not to ER, PR or Her-2 status (P>0.05).


CONCLUSIONS
D2-40 can specifically mark the lymphatic endothelial cells in invasive ductal carcinoma of the breast. The LMD and MD are positively correlated to the clinicopathological factors of the malignancy. D2-40 and CD34 has the potential for use as the predictors to evaluate the tumor progression and metastasis.
Zhen
Le-feng
LF
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lok_zhen@163.com
Ye
Chang-sheng
CS
Liu
Min-feng
MF
Ding
Gui-po
GP
Liao
Jia-jian
JJ
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D042601:Lymphatic Vessels; D055806:Microvessels; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D055815:Young Adult
D018952:Antigens, CD34; D014408:Biomarkers, Tumor