Cloning and characterization of a Lentinula edodes class II chitin synthase gene, LeChs2.
A genomic DNA sequence and cDNA encoding a putative chitin synthase were isolated from the white rot basidiomycete Lentinula edodes. The gene, named LeChs2, consists of a 2,598-bp open reading frame interrupted by 14 introns and encodes a putative protein of 866 amino acid residues. The data obtained in this study suggest that LeChs2 belongs to the class II chitin synthases.
Sato
Toshitsugu
T
Iwate Biotechnology Research Center, Narita, Kitakami, Iwate, Japan. tosisato@mail.kitami-it.ac.jp
Okawa
Kumiko
K
Hirano
Tatsuya
T
D015152:Blotting, Northern; D002687:Chitin Synthase / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000235:genetics*
D002687:Chitin Synthase; C400821:chitin synthase 2