Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor.
Lipopolysaccharides free P[3-hydroxybutyrate (3HB)-co-3-hydroxyvalerate (3HV)] production was achieved using recombinant Corynebacterium glutamicum harboring polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha. Cells grown on glucose with feeding of propionate as a precursor of 3HV unit accumulated 8-47wt% of P(3HB-co-3HV). The 3HV fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations.
Matsumoto
Ken'ichiro
K
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan. mken@eng.hokudai.ac.jp
Kitagawa
Kei
K
Jo
Sung-Jin
SJ
Song
Yuyang
Y
Taguchi
Seiichi
S
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D020118:Cupriavidus necator / Q000235:genetics; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis / Q000235:genetics*; D011422:Propionates / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011422:Propionates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates