[The role of interleukin-1 gene polymorphism in gastric carcinogenesis associated with Helicobacter pylori infection].
BACKGROUND
The search for risk factors of gastric cancer has a long history: H. pylori infection is one of them. Unfortunately, there is no reliable method for the identification of patients at significant risk of gastric cancer among those infected.


OBJECTIVE
To disclose the role of IL-1B gene polymorphism in gastric cancer by comparing IL-1B genotypes of patients with different, almost mutually exclusive clinical courses.


METHODS
We initially enrolled 273 patients and continued with 259 patients who tested positive for antibodies against H. pylori, including 121 patients with gastric cancer, 119 patients with duodenal ulcer, and 19 patients with asymptomatic gastritis. The study group included 19 families (48 patients) with 14 cases of gastric cancer, 15 cases of duodenal ulcer, and 19 asymptomatic individuals.


METHODS
The IL-1B gene was sequenced at position -511 and -31 using primer extension and mass spectrometry. Intron 2 of the IL-1RN gene was studied using PCR and agarose gel electrophoresis. The odds ratio (OR) with 95% confidence intervals was computed using logistic regression.


RESULTS
No association was found between IL-1B gene polymorphism and clinical presentation in 19 families. The differences between patients with gastric cancer and duodenal ulcer applied only to the C/T variant at position -511 of the IL-1B gene after adjustment for family history. Patients with the IL-1B-511 CT genotype and negative family history of gastric cancer demonstrated a greater risk of gastric cancer compared with patients with positive family history.


CONCLUSIONS
Our results suggest a complex association between IL-1B gene polymorphism, H. pylori infection, and gastric secretion.
Brzeźniakiewicz
Katarzyna
K
Klinika Gastroenterologii Pomorskiej Akademii Medycznej w Szczecinie ul. Unii Lubelskiej 1, 71-252 Szczecin.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015984:Causality; D015897:Comorbidity; D004381:Duodenal Ulcer / Q000453:epidemiology; D005753:Gastric Mucosa / Q000378:metabolism; D005756:Gastritis / Q000453:epidemiology; D005787:Gene Frequency; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000378:metabolism; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D016015:Logistic Models; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000378:metabolism
D053583:Interleukin-1beta