title: Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV).
abstract: We report the effect of two probiotics, Lactobacil and Sporolac as separate or mixed diets on innate immune mechanisms, such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity, and disease resistance in lymphocystis disease virus (LCDV) infected olive flounder, Paralichthys olivaceus (523.5 +/- 18.3 g) on week 1, 2, and 4. In infected fish, administration of diet supplemented with Lactobacil individually or mixed with Sporolac significantly enhanced the immune parameters like phagocytic activity superoxide anion production, complement activity, and plasma lysozyme. However administration of supplemented diet with Sporolac alone, all the chosen immune parameters did not enhance when compared to control group; this diets resulted in lower mortality (30% and 25%) than Sporolac diet group (45%) in 30 days. We conclude that Lactobacil individually or mixed with Sporolac act as immunostimulants that enhance the innate immune response and disease resistance in lymphocystis disease virus (LCDV) infected olive flounder.
authors.affiliation: Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University, Jeju 690 756, South Korea. rhari123@yahoo.com
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Harikrishnan
authors.forename: Chellam
authors.initials: C
authors.lastname: Balasundaram
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Heo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D004266:DNA Virus Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D019587:Dietary Supplements; D005393:Fish Diseases / Q000188:drug therapy / Q000276:immunology* / Q000821:virology*; D005432:Flounder; D007113:Immunity, Innate / Q000187:drug effects*; D007497:Iridoviridae / Q000276:immunology*; D009113:Muramidase / Q000097:blood; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D009113:Muramidase