[The systemic evaluation and clinical significance of immunological function for advanced lung cancer patients].
OBJECTIVE
The actual evaluation of immunological function is significant for studing the tumor development and devising a treatment in time. The aim of this study is to evaluate the immunological function of advanced lung cancer patients systematically, and to discuss the clinical significance.


METHODS
The nucleated cell amounts of advanced lung cancer patients and the healthy individuals were counted. The immune cell subsets and the levels of IL-4, INF-gamma, perforin and granzyme in CD8+T cells by the flow cytometry were measured. The proliferation activity and the inhibition ratio of immune cells to several tumor cell lines were evaluated by MTT assay.


RESULTS
The absolute amounts and subsets of T, B, NK cells of advanced lung cancer patients were lower than the healthy individuals (P < 0.05); However, the proportion of regulatory T cells of advanced lung cancer patients (4.00 +/- 1.84)% was lower than the healthy individuals (1.27 +/- 0.78)% (P < 0.05). The positive rates of IFN-gamma perforin, granzyme in CD8+T cells decreased while them in IL-4 did not in the advanced lung cancer patients compared to the healthy control group (P < 0.05). The proliferation activity of immune cells, the positive rate of PPD masculine and the inhibition ratio to tumor cells in the advanced lung cancer patients was lower than the healthy subsets obviously (P < 0.05).


CONCLUSIONS
There was a significant immune depression in the advanced lung cancer patients compared to the healthy individuals.
Wulan
Tuya
T
Department of Biotherapy, Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.
Wang
Shiyong
S
Du
Weili
W
Zhang
Hui
H
Zhang
Yuan
Y
Zeng
Xue
X
Liu
Sa
S
Liu
Yanping
Y
Zhang
Lu
L
Zhang
Zhe
Z
He
Ying
Y
Wang
Jialing
J
Wu
Xiuyan
X
D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054353:Perforin / Q000378:metabolism
D054353:Perforin; D015847:Interleukin-4; D007371:Interferon-gamma; D053804:Granzymes