The bHLH transcription factor POPEYE regulates response to iron deficiency in Arabidopsis roots.
Global population increases and climate change underscore the need for better comprehension of how plants acquire and process nutrients such as iron. Using cell type-specific transcriptional profiling, we identified a pericycle-specific iron deficiency response and a bHLH transcription factor, POPEYE (PYE), that may play an important role in this response. Functional analysis of PYE suggests that it positively regulates growth and development under iron-deficient conditions. Chromatin immunoprecipitation-on-chip analysis and transcriptional profiling reveal that PYE helps maintain iron homeostasis by regulating the expression of known iron homeostasis genes and other genes involved in transcription, development, and stress response. PYE interacts with PYE homologs, including IAA-Leu Resistant3 (ILR3), another bHLH transcription factor that is involved in metal ion homeostasis. Moreover, ILR3 interacts with a third protein, BRUTUS (BTS), a putative E3 ligase protein, with metal ion binding and DNA binding domains, which negatively regulates the response to iron deficiency. PYE and BTS expression is also tightly coregulated. We propose that interactions among PYE, PYE homologs, and BTS are important for maintaining iron homeostasis under low iron conditions.
Long
Terri A
TA
Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, North Carolina 27708, USA.
Tsukagoshi
Hironaka
H
Busch
Wolfgang
W
Lahner
Brett
B
Salt
David E
DE
Benfey
Philip N
PN
D017360:Arabidopsis / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000502:physiology*; D020869:Gene Expression Profiling; D006706:Homeostasis; D007501:Iron / Q000378:metabolism; D000090463:Iron Deficiencies; D018517:Plant Roots / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D051792:Basic Helix-Loop-Helix Proteins; D007501:Iron