title: Toll-like receptor 8 and 9 polymorphisms in Crimean-Congo hemorrhagic fever.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is an acute viral hemorrhagic fever. The clinical course and outcome of the CCHF infection are different in humans. Toll-like receptors (TLRs) are a family of pathogen recognition receptors. TLR8 and TLR9 contribute to the recognition of viruses. We investigated frequency of TLR8 Met1Val, TLR8 -129C/G, TLR9 -1486T/C and TLR9 2458G/A polymorphisms in CCHF patients and healthy controls. Our study was conducted between June 1 and August 31, 2007 in Cumhuriyet University Hospital, Turkey. TLR genotypes were detected using the PCR-RFLP assay in 85 CCHF patients and 171 healthy controls. We found that heterozygous plus homozygous mutant genotypes frequency for TLR8 Met1Val and for TLR9 -1486T/C were significantly higher in CCHF patients than controls (p = 0.038 and p = 0.009, respectively). The frequency of TLR8 -129G/G genotype in the fatal CCHF patients was significantly higher than that of the non-fatal patients (p = 0.026). The frequency of TLR9 -1486C/C genotype was significantly higher in fatal CCHF patients than in healthy controls (p = 0.009) and in patients with severe disease compared to non-severe disease (p = 0.044). Our findings suggest that TLR8 Met1Val, TLR8 -129C/G, and TLR9 -1486T/C polymorphisms are important on clinical course of CCHF disease.
authors.affiliation: Department of Infectious Diseases, Cumhuriyet University, School of Medicine, 58140 Sivas, Turkey. aynurum2000@yahoo.com
authors.forename: Aynur
authors.initials: A
authors.lastname: Engin
authors.forename: Serdal
authors.initials: S
authors.lastname: Arslan
authors.forename: Sibel
authors.initials: S
authors.lastname: Kizildag
authors.forename: Hasret
authors.initials: H
authors.lastname: Oztürk
authors.forename: Nazif
authors.initials: N
authors.lastname: Elaldi
authors.forename: Ilyas
authors.initials: I
authors.lastname: Dökmetas
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bakir
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006479:Hemorrhagic Fever, Crimean / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051200:Toll-Like Receptor 8 / Q000235:genetics*; D051217:Toll-Like Receptor 9 / Q000235:genetics*; D014421:Turkey
chemical_list: C495349:TLR8 protein, human; C495371:TLR9 protein, human; D051200:Toll-Like Receptor 8; D051217:Toll-Like Receptor 9