Glutamine protects ischemia-reperfusion induced acute lung injury in isolated rat lungs.
Glutamine has been used to treat a number of diseases via modulating the inflammatory response. The purpose of this study is to investigate whether glutamine has a beneficial effect in ischemia-reperfusion (IR) induced acute lung injury in an isolated rat lung model. Typical acute lung injury in rats was successfully induced by 60 min of ischemia and 60 min of reperfusion. At the end of experiment, bronchoalveolar lavage fluid (BALF), perfusate and lung tissues were collected to evaluate the degree of lung injury. Glutamine (20 mM) was administrated before ischemia or after ischemia. IR caused a significant increase in the capillary filtration coefficient; lung weight gain; lung weight to body weight ratio; wet to dry weight ratio; pulmonary arterial pressure; and protein concentration and lactate dehydrogenase level in BALF. Tumor necrosis factor-α and cytokine induced neutrophil chemoattractant-1 in perfusate, and malondialdehyde levels, carbonyl content and myeloperoxidase activities in lung tissue were also significantly increased. In addition, the lung tissues showed increased septal thickness and neutrophil infiltration. Furthermore, NF-κB activity and degradation of IκB-α were significantly increased in the lungs. Treatment with glutamine before ischemia or after ischemia significantly decreased the increase in these parameters. Our study showed that glutamine treatment decreased IR-induced acute lung injury. The protective mechanism may be due to the inhibition of NF-κB activation and the attenuation of oxidative stress.
Peng
Chung-Kan
CK
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
Huang
Kun-Lun
KL
Wu
Chin-Pyng
CP
Li
Min-Hui
MH
Hu
Ya-Ting
YT
Hsu
Ching-Wang
CW
Tsai
Shih-Hung
SH
Chu
Shi-Jye
SJ
D055371:Acute Lung Injury / Q000517:prevention & control*; D000818:Animals; D005973:Glutamine / Q000494:pharmacology*; D008168:Lung / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009929:Organ Size; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D005973:Glutamine; D009195:Peroxidase