RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells.
Murine embryonic stem cells (ES) are pluripotent cells and have the potential to become a wide variety of specialized cell types. Mouse ES cell differentiation can be regarded as a valuable biological tool that has led to major advances in our understanding of cell and developmental biology. In vitro differentiation of mouse ES cells can be directed to a specific lineage formation, such as hematopoietic lineage, by appropriate cytokine and/or growth factor stimulation. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used; however, this is a time-consuming and expensive approach. Recently, we have shown that siRNA is an effective strategy to knockdown target gene expression, such as Ape1, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This approach will be applicable to test the function of a wide variety of gene products using the ES cell differentiation system.
Zou
Gang-Ming
GM
Department of Pathology and Otolaryngology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Lebron
Cynthia
C
Fu
Yumei
Y
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D010084:Oxidation-Reduction; D034622:RNA Interference / Q000502:physiology*
C486216:Apex1 protein, mouse; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase