In vitro human TGF-beta treatment converts CD4(+)CD25(-) T cells into induced T regulatory like cells.
In mammals, induced T regulatory cells (iTregs) are produced by treating CD4(+)CD25(-) cells in vitro with TGF-beta in the presence of anti-CD3 and IL-2. Chicken CD4(+)CD25(-) cells were treated in vitro with TGF-beta, anti-CD3, anti-CD28 and IL-2 and analyzed for suppressive properties, IL-10 and IL-2 mRNA amounts. TGF-beta treatment decreased (P<0.01) CD4(+)CD25(-) cell proliferation by 45% at 5d of cell culture with no increase in dead cell population. CD4(+)CD25(-) cells treated with 5ng TGF-beta for 5d had 130-fold (P<0.01) more IL-10 and 104-fold (P<0.01) less IL-2 mRNA content than the 0ng TGF-beta treated groups. CD4(+)CD25(-) cells treated with 5ng TGF-beta for 5d suppressed (P<0.01) naïve cell proliferation compared to the 0ng TGF-beta treated groups. The results suggest the presence of iTreg-like cells in avian species.
Shanmugasundaram
Revathi
R
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, United States.
Selvaraj
Ramesh K
RK
D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10