Serum proteomic analysis from bacteremic and leucopenic rabbits.
BACKGROUND
Bacteremia causes mild to life-threatening illnesses as a consequence of inflammatory pathway activation, and leucopenia can increase mortality rates and treatment costs. The identification of novel biomarkers for leucopenia in the context of bacteremia would facilitate early detection and provide insights into the identification of novel therapeutic targets.


METHODS
Rabbits were infected with Klebsiella pneumoniae to induce bacteremia with leucopenia, and sera from infected and noninfected rabbits was subjected to proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to compare serum protein profiles.


RESULTS
Five differentially-expressed protein spots were identified in bacteremic rabbits with leucopenia compared to protein expression profiles prior to infection. The proteins were identified as desmin, cystic fibrosis transmembrane conductance regulator (CFTR), interleukin (IL) 4, and the interleukin 6 precursor. The increased levels of desmin and CFTR in serum were further confirmed by Western blot analysis.


CONCLUSIONS
These results indicated that desmin, CFTR, IL-4, and the IL-6 precursor may be potential molecular biomarkers for leucopenia occurring with bacteremia.
Zhou
Zheng
Z
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, PR China.
Ren
Jianan
J
Liu
Haiyan
H
Gu
Guosheng
G
Li
Jieshou
J
D000818:Animals; D016470:Bacteremia / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D007710:Klebsiella Infections / Q000276:immunology / Q000378:metabolism*; D007711:Klebsiella pneumoniae; D007970:Leukopenia / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011817:Rabbits; D015854:Up-Regulation / Q000276:immunology
D015415:Biomarkers; D001798:Blood Proteins