Molecular characterization and expression of the MYND-ZF gene from Clonorchis sinensis.
The MYND-type zinc finger protein (MYND-ZF) is a large group of proteins containing the MYND domain which play an important role in protein-protein interactions. A cDNA clone encoding a novel MYND-ZF was isolated and identified from a Clonorchis sinensis (C. sinensis) adult cDNA library. The open reading frame of this novel cDNA sequence contains 1,440 base pairs with a putative protein of 479 amino acids showing a high homology with the MYND-ZF identified from other species. Recombinant CsMYND-ZF was expressed and purified from Escherichia coli BL21 (DE3). CsMYND-ZF transcripts were detected in the cDNA of adult worms and metacercariae but not in eggs of C. sinensis. Immunohistochemistry results revealed that CsMYND-ZF was deposited at the tegument of adult worms and metacercariae C. sinensis using anti-recombinant CsMYND-ZF serum. These findings may contribute to the development of a reliable diagnostic method.
Wang
Lexun
L
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 2nd Zhongshan Road, Guangzhou 510089, China.
Yu
Xinbing
X
Yang
Yabo
Y
Chen
Jingfang
J
Hu
Dong
D
Deng
Chuanhuan
C
Yang
Xiao
X
Hu
Xuchu
X
Xu
Jin
J
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015801:Helminth Proteins / Q000032:analysis / Q000235:genetics*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers / Q000235:genetics
D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins