title: Multiclonal epidemic of Klebsiella pneumoniae isolates producing DHA-1 in a Spanish hospital.
abstract: Between June 2007 and January 2008, 26 Klebsiella pneumoniae isolates carrying bla(DHA-1) on an IncL/M plasmid were obtained from clinical samples at Granollers Hospital, Barcelona, Spain. Three of the isolates also carried a bla(CTX-M-15) gene. The 26 isolates showed 11 pulsed-field gel electrophoresis (PFGE) patterns. Multilocus sequence typing showed that PFGE patterns A, B and C belonged to sequence type (ST)17, D to ST13, E to ST427, F and G to ST416, H to ST37, I to ST440, J to ST326, and K to ST428. Results demonstrated the effectiveness of the infection control programme in place at the centre. This study reports the first characterization of STs for bla(DHA-1) -producing K. pneumoniae isolates.
authors.affiliation: Servei de Microbiologia, Hospital de Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: K
authors.initials: K
authors.lastname: Diestra
authors.forename: E
authors.initials: E
authors.lastname: Miró
authors.forename: C
authors.initials: C
authors.lastname: Martí
authors.forename: D
authors.initials: D
authors.lastname: Navarro
authors.forename: J
authors.initials: J
authors.lastname: Cuquet
authors.forename: P
authors.initials: P
authors.lastname: Coll
authors.forename: F
authors.initials: F
authors.lastname: Navarro
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005798:Genes, Bacterial; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017053:Infection Control / Q000379:methods; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D010957:Plasmids; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001618:beta-Lactamases