Detecting protein interactions in live cells via complementation of a hydrolysis-deficient β-lactamase.
Protein-fragment complementation assay using a hydrolysis-deficient β-lactamase provides a robust tool for studying protein-protein interactions.
Jiang
Jiangsong
J
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Zhang
Junxiang
J
Li
Shuwei
S
D055162:Biocatalysis; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D008968:Molecular Conformation; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D011506:Proteins; D001618:beta-Lactamases