Raman-assisted crystallography of biomolecules at the synchrotron: instrumentation, methods and applications.
Raman spectroscopy is a powerful technique that, in recent years, has been successfully coupled to X-ray crystallography for the analysis of biological macromolecular systems. The complementarity between both techniques is illustrated at multiple stages, including sample preparation, data collection and structural interpretation with a mechanistic perspective. The current state of instrumentation is described, focusing on synchrotron based setups. Present and future applications of Raman microspectrophotometry are reviewed with reference to recent examples dealing with metallo-, photosensitive-, and redox-proteins. The added value of Raman microspectrophotometry to assess X-radiation damage is discussed, and its applicability to investigate crystalline DNA molecules is also emphasized. This article is part of a Special Issue entitled: Protein Structure and Function in the Crystalline State.
McGeehan
John E
JE
Biophysics Laboratories, Institute of Biomedical and Biomolecular Sciences, School of Biological Sciences, University of Portsmouth, PO1 2DY, UK.
Bourgeois
Dominique
D
Royant
Antoine
A
Carpentier
Philippe
P
D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D015591:Microspectrophotometry / Q000295:instrumentation / Q000379:methods*; D008958:Models, Molecular; D009696:Nucleic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D017356:Synchrotrons
D009696:Nucleic Acids; D011506:Proteins