Bone regeneration by bioactive hybrid membrane containing FGF2 within rat calvarium.
This study examined the bone regeneration potential of a novel hybrid membrane consisting of collagen and nano-bioactive glass (nBG) incorporating basic fibroblast growth factor (FGF2) for use in guided bone regeneration. nBG was added to a reconstitution of collagen at a concentration of 30%, and the hybrid was formulated into a thin membrane. FGF2 (50 microg/ml) was adsorbed to the hybrid membrane. This level of FGF2 was found to be the optimal concentration to stimulate osteoblastic differentiation in vitro. Three membrane groups, including pure collagen, collagen-nBG hybrid and its combination with FGF2 were implanted within a rat calvarium defect (phi = 5 mm) for a period of 3 weeks. Histomorphometric analysis was carried out to evaluate the bone regeneration within the defect. The results showed that the defect in the collagen-nBG-FGF2 membrane was recovered almost completely, while partial recovery was observed in the other membrane groups (collagen and collagen-BG). However, there was little defect recovery in the blank control. The new bone formation was as high as approximately 60, approximately 45, and approximately 30% of the defect treated with the collagen-nBG-FGF2, collagen-BG, and collagen, respectively, whilst only 4% of new bone was observed in the blank control. Overall, the nBG was shown to stimulate bone formation of the collagen membrane, and FGF2 synergistically accelerated the bone regeneration within a rat calvarium defect.
Hong
Ki Seok
KS
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea.
Kim
Eun-Cheol
EC
Bang
So-Hee
SH
Chung
Chin-Hyung
CH
Lee
Young Il
YI
Hyun
Jung Keun
JK
Lee
Hae-Hyoung
HH
Jang
Jun-Hyeog
JH
Kim
Tae-Il
TI
Kim
Hae-Won
HW
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001681:Biological Assay; D001861:Bone Regeneration / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D016222:Fibroblast Growth Factor 2