Application of a novel co-enzyme reactor in chemiluminescence flow-through biosensor for determination of lactose.
A novel enzyme reactor with co-immobilization of beta-galactosidase and glucose oxidase in calcium alginate fiber (CAF) and amine modified nanosized mesoporous silica (AMNMS) was prepared which incorporate the adsorption and catalysis of AMNMS with the cage effect of the polymer to increase catalytic activity and stability of immobilized enzyme. The enzyme reactor was applied to prepare a chemiluminescence (CL) flow-through biosensor for determination of lactose combined with a novel luminol-diperiodatonickelate (DPN) CL system we reported. It shows that the CL flow-through biosensor possesses long lifetime, high stability, high catalytic activity and sensitivity. The relative CL intensity was linear with the lactose concentration in the range of 8 x 10(-8) - 4 x 10(-6) g mL(-1) with the detection limit of 2.7 x 10(-8) g mL(-1) (3sigma). It has been successfully applied to the determination of lactose in milk.
Yang
Chunyan
C
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
Zhang
Zhujun
Z
Shi
Zuolong
Z
Xue
Pan
P
Chang
Pingping
P
Yan
Ruifang
R
D000327:Adsorption; D000464:Alginates / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D000818:Animals; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007785:Lactose / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008892:Milk / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
D000464:Alginates; D000588:Amines; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D012822:Silicon Dioxide; D020723:Glucuronic Acid; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D007785:Lactose