title: [Molecular characterization and evolutional analysis of liportein lipase and hepatic lipase gene in Chinese Sturgeon and other six freshwater fishes].
abstract: In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Xu-Fang
authors.initials: XF
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Guang-Zhao
authors.initials: GZ
authors.lastname: Li
authors.forename: Xiu-Xia
authors.initials: XX
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps; D006224:Cricetinae / Q000145:classification / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D019143:Evolution, Molecular; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005618:Fresh Water; D008049:Lipase / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D008049:Lipase; D008071:Lipoprotein Lipase