title: Effects of lead and mercury on the blood proteome of children.
abstract: Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.
authors.affiliation: Department of Chemistry, SUNY Oswego, Oswego, New York 13126, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Birdsall
authors.forename: Michael P
authors.initials: MP
authors.lastname: Kiley
authors.forename: Zaneer M
authors.initials: ZM
authors.lastname: Segu
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Palmer
authors.forename: Milan
authors.initials: M
authors.lastname: Madera
authors.forename: Brooks B
authors.initials: BB
authors.lastname: Gump
authors.forename: James A
authors.initials: JA
authors.lastname: MacKenzie
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Parsons
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: Kestutis G
authors.initials: KG
authors.lastname: Bendinskas
mesh_terms: D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000097:blood / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D002853:Chromatography, Liquid; D003430:Cross-Sectional Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007855:Lead Poisoning / Q000097:blood*; D008297:Male; D008630:Mercury Poisoning / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry; D020543:Proteome / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012737:Sex Factors; D012959:Socioeconomic Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000097:blood / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D001798:Blood Proteins; D010446:Peptide Fragments; D020543:Proteome; D014357:Trypsin