title: Yields of clustered DNA damage induced by charged-particle radiations of similar kinetic energy per nucleon: LET dependence in different DNA microenvironments.
abstract: To determine the linear energy transfer (LET) dependence of the biological effects of densely ionizing radiation in relation to changes in the ionization density along the track, we measured the yields and spectrum of clustered DNA damages induced by charged particles of different atomic number but similar kinetic energy per nucleon in different DNA microenvironments. Yeast DNA embedded in agarose in solutions of different free radical scavenging capacity was irradiated with 1 GeV protons, 1 GeV/nucleon oxygen ions, 980 MeV/nucleon titanium ions or 968 MeV/nucleon iron ions. The frequencies of double-strand breaks (DSBs), abasic sites and oxypurine clusters were quantified. The total DNA damage yields per absorbed dose induced in non-radioquenching solution decreased with LET, with minor variations in radioquenching conditions being detected. However, the total damage yields per particle fluence increased with LET in both conditions, indicating a higher efficiency per particle to induce clustered DNA damages. The yields of DSBs and non-DSB clusters as well as the damage spectra varied with LET and DNA milieu, suggesting the involvement of more than one mechanism in the formation of the different types of clustered damages.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA. keszenman@bnl.gov
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Keszenman
authors.forename: Betsy M
authors.initials: BM
authors.lastname: Sutherland
mesh_terms: D004249:DNA Damage / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D018534:Earth, Planet; D004781:Environmental Exposure; D006801:Humans; D007700:Kinetics; D018499:Linear Energy Transfer; D011839:Radiation, Ionizing; D015003:Yeasts / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D004271:DNA, Fungal