title: Immune enhancement of chemotherapeutants on lymphocystis disease virus (LDV) infected Paralichthys olivaceus.
abstract: In olive flounder, Paralichthys olivaceus infected with lymphocystis disease virus (LDV) bath treatment with formalin, hydrogen peroxide or Jenoclean at 50, 100, and 200 ppm daily 10 min for 60 days enhanced the innate immune response and disease resistance. Jenoclean enhanced the immune parameters at the lowest concentration of 50 ppm; on the other hand, hydrogen peroxide bath treatment enhanced the immunity level at 100 ppm, while formalin was effective only at 200 ppm. A low cumulative mortality and high relative percent survival was noted in Jenoclean treated group followed by formalin and hydrogen peroxide treated groups. In olive flounder at low concentrations of 50 ppm or 100 ppm hydrogen peroxide and Jenoclean effectively prevent LDV infection.
authors.affiliation: Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Marine Biomedical Sciences & Marine and Environmental Research Institute, College of Ocean Science, Jeju National University, Jeju 690 756, South Korea. rhari123@yahoo.com
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Harikrishnan
authors.forename: Man-Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Ju-Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Chellam
authors.initials: C
authors.lastname: Balasundaram
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Heo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003170:Complement Pathway, Alternative / Q000276:immunology; D004266:DNA Virus Infections / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000188:drug therapy / Q000276:immunology* / Q000821:virology*; D005432:Flounder; D005557:Formaldehyde / Q000494:pharmacology / Q000627:therapeutic use; D006861:Hydrogen Peroxide / Q000494:pharmacology / Q000627:therapeutic use; D007113:Immunity, Innate / Q000187:drug effects*; D007497:Iridoviridae / Q000276:immunology*; D009113:Muramidase / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D016897:Respiratory Burst / Q000276:immunology; D016019:Survival Analysis
chemical_list: D005557:Formaldehyde; D006861:Hydrogen Peroxide; D009113:Muramidase