title: The biological activities of (1,3)-(1,6)-beta-d-glucan and porous electrospun PLGA membranes containing beta-glucan in human dermal fibroblasts and adipose tissue-derived stem cells.
abstract: In this study, we investigated the possible roles of (1,3)-(1,6)-beta-d-glucan (beta-glucan) and porous electrospun poly-lactide-co-glycolide (PLGA) membranes containing beta-glucan for skin wound healing, especially their effect on adult human dermal fibroblast (aHDF) and adipose tissue-derived stem cell (ADSC) activation, proliferation, migration, collagen gel contraction and biological safety tests of the prepared membrane. This study demonstrated that beta-glucan and porous PLGA membranes containing beta-glucan have enhanced the cellular responses, proliferation and migration, of aHDFs and ADSCs and the result of a collagen gel contraction assay also revealed that collagen gels contract strongly after 4 h post-gelation incubation with beta-glucan. Furthermore, we confirmed that porous PLGA membranes containing beta-glucan are biologically safe for wound healing study. These results indicate that the porous PLGA membranes containing beta-glucan interacted favorably with the membrane and the topical administration of beta-glucan was useful in promoting wound healing. Therefore, our study suggests that beta-glucan and porous PLGA membranes containing beta-glucan may be useful as a material for enhancing wound healing.
authors.affiliation: Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea.
authors.forename: Yeon I
authors.initials: YI
authors.lastname: Woo
authors.forename: Bong Joo
authors.initials: BJ
authors.lastname: Park
authors.forename: Hye-Lee
authors.initials: HL
authors.lastname: Kim
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Jungsung
authors.initials: J
authors.lastname: Kim
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Yang
authors.forename: Jung Koo
authors.initials: JK
authors.lastname: Kim
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Tsubaki
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Han
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D001684:Biological Dressings; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005936:Glucans / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D012399:Rotation; D012867:Skin / Q000166:cytology; D012879:Skin Physiological Phenomena; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation; D014945:Wound Healing / Q000502:physiology
chemical_list: D005936:Glucans; D008567:Membranes, Artificial; C033671:epiglucan; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid