[Contributions of putative lipoate-protein ligase to the virulence of Streptococcus pneumoniae].
OBJECTIVE
To investigate the effect of putative lipoate-protein ligase (LPL) on the virulence of Streptococcus pneumoniae.


METHODS
lpl gene deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The cell adherence assay and mice challenge assay were used to observe the differences between wild strain and the mutant in the pathopoiesis of Streptococcus pneumoniae.


RESULTS
Mice virulence experiments showed that the median lethal time of wide type and the lpl mutant are both 12 h, no statistics difference. The ability of adherence of the mutant was greater than the wild strain (P < 0.01); The capsule stain in-vivo showed that the wild strain and the mutant both had the capsule.


CONCLUSIONS
lpl gene inhibits the adherence to host, but no affect on the ability to infection mice by
Yang
Xiaoliang
X
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 400016, China. yangxiaoliang2010@yeah.net
Yin
Nanlin
N
Pang
Dan
D
Wu
Kaifeng
K
Yin
Yibing
Y
Zhang
Xuemei
X
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D011008:Pneumococcal Infections / Q000382:microbiology* / Q000401:mortality; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
D001426:Bacterial Proteins; D010453:Peptide Synthases; C072322:lipoate-protein ligase