Development and validation of a sandwich ELISA for the determination of potentially allergenic sesame (Sesamum indicum) in food.
This paper presents a sandwich enzyme-linked immunosorbent assay (ELISA) that allows the determination of traces of sesame in food. Chicken anti-sesame antibodies, used as coating antibodies, and rabbit anti-sesame antibodies, used as secondary antibodies, were prepared by immunization with a protein extract of white, peeled sesame. The ELISA did not show any cross-reactivity with 19 food ingredients commonly found in sesame-containing foodstuffs such as seeds, nuts, and cereals. In whole grain bread, crisp toast, and snacks, the limit of detection (S/N = 3) was 0.5, 0.5, and 0.3 μg sesame protein/g, and the limit of quantification (S/N = 10) was 0.6, 0.8, and 1.4 μg sesame protein/g, respectively. The analysis of blank food matrices (whole grain bread, white bread, crisp toast, and snacks) spiked with sesame protein at four spike levels generally resulted in mean recoveries from 72% to 145%. In the case of spiking blank food matrices with sesame seeds, the ELISA proved to be more accurate for whole wheat cookies than for whole wheat bread.
Redl
Gerda
G
Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.
Husain
Fatima T
FT
Bretbacher
Ines E
IE
Nemes
Albert
A
Cichna-Markl
Margit
M
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology; D012639:Seeds / Q000737:chemistry / Q000276:immunology; D031685:Sesamum / Q000737:chemistry* / Q000276:immunology
D000485:Allergens; D010940:Plant Proteins