Evaluation of biocompatible stabilised gelled soya bean oil nanoparticles as new hydrophobic reservoirs.
Based on the organogel concept, in which an oil is trapped in a network of low-molecular-mass organic gelator fibres creating a gel, a formulation of gelled soya bean oil nanoparticles was evaluated for its capacity to form biocompatible hydrophobic reservoirs. The aqueous dispersions of nanoparticles were prepared by hot emulsification (T° > Tgel) and cooling at room temperature in the presence of polyethyleneimine (PEI). The dispersions were stabilised by the electrostatic interactions between the positively charged amino groups of the PEI and the negatively charged carboxylates of the gelator fibres present at the surface of the particles. The aqueous dispersions were highly stable (several months) and the gelled particles were able to entrap a hydrophobic fluorescent model molecule (Nile red), allowing testing in cells. The gelled oil nanoparticles were found to be biocompatible with the tested cells (keratinocytes) and had the ability to become rapidly internalised. Thus, organogel-based nanoparticles are a promising hydrophobic drug delivery system.
Boudier
Ariane
A
Laboratoire IMRCP, UMR 5623, Universite Paul Sabatier, Toulouse Cedex, France.
Kirilov
Plamen
P
Franceschi-Messant
Sophie
S
Belkhelfa
Haouaria
H
Hadioui
Laila
L
Roques
Christine
C
Perez
Emile
E
Rico-Lattes
Isabelle
I
D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D004563:Electrochemistry; D005456:Fluorescent Dyes; D005782:Gels; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D015603:Keratinocytes / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010078:Oxazines; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013778:Tetrazolium Salts; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D005782:Gels; D007202:Indicators and Reagents; D010078:Oxazines; D013778:Tetrazolium Salts; D014867:Water; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D013024:Soybean Oil; D011094:Polyethyleneimine; C044808:nile red