title: Transcriptional profiling of XdrA, a new regulator of spa transcription in Staphylococcus aureus.
abstract: Transcription of spa, encoding the virulence factor protein A in Staphylococcus aureus, is tightly controlled by a complex regulatory network, ensuring its temporal expression over growth and at appropriate stages of the infection process. Transcriptomic profiling of XdrA, a DNA-binding protein that is conserved in all S. aureus genomes and shares similarity with the XRE family of helix-turn-helix, antitoxin-like proteins, revealed it to be a previously unidentified activator of spa transcription. To assess how XdrA fits into the complex web of spa regulation, a series of regulatory mutants were constructed; consisting of single, double, triple, and quadruple mutants lacking XdrA and/or the three key regulators previously shown to influence spa transcription directly (SarS, SarA, and RNAIII). A series of lacZ reporter gene fusions containing nested deletions of the spa promoter identified regions influenced by XdrA and the other three regulators. XdrA had almost as strong an activating effect on spa as SarS and acted on the same spa operator regions as SarS, or closely overlapping regions. All data from microarrays, Northern and Western blot analyses, and reporter gene fusion experiments indicated that XdrA is a major activator of spa expression that appears to act directly on the spa promoter and not through previously characterized regulators.
authors.affiliation: Institute of Medical Microbiology, University of Zurich, Gloriastr. 32, 8006 Zurich, Switzerland. mccallum@imm.uzh.ch
authors.forename: N
authors.initials: N
authors.lastname: McCallum
authors.forename: J
authors.initials: J
authors.lastname: Hinds
authors.forename: M
authors.initials: M
authors.lastname: Ender
authors.forename: B
authors.initials: B
authors.lastname: Berger-Bächi
authors.forename: P
authors.initials: P
authors.lastname: Stutzmann Meier
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015152:Blotting, Northern; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology; D006461:Hemolysis / Q000187:drug effects; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D012329:RNA, Bacterial / Q000235:genetics; D012756:Sheep; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; C096035:RNAIII, Staphylococcus aureus; C492107:SarA protein, Staphylococcus aureus; C476149:SarS protein, Staphylococcus aureus