title: Detecting protein interactions in live cells via complementation of a hydrolysis-deficient β-lactamase.
abstract: Protein-fragment complementation assay using a hydrolysis-deficient β-lactamase provides a robust tool for studying protein-protein interactions.
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
authors.forename: Jiangsong
authors.initials: J
authors.lastname: Jiang
authors.forename: Junxiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Shuwei
authors.initials: S
authors.lastname: Li
mesh_terms: D055162:Biocatalysis; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D008968:Molecular Conformation; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D001618:beta-Lactamases