Boron regulates mineralized tissue-associated proteins in osteoblasts (MC3T3-E1).
The aim of this study was to determine the effects of boron (B) on the cell-survival, proliferation, mineralization and mRNA expression of mineralized tissue-associated proteins. Additionally, determination of the effects of B on the BMP-4, -6 and -7 protein levels of pre-osteoblastic cells (MC3T3-E1) was also intended. The effects of B (pH 7.0) concentrations (0, 0.1, 1, 10, 100, 1000, 2000, 4000, 8000 and 10,000 ng/ml) on the survival of the cells were evaluated at 24 and 96 hrs with MTT assay. To evaluate the proliferation in long term, MC3T3-E1 cells were treated with different concentrations of B (0, 0.1, 1, 10, 100 and 1000 ng/ml) and were counted on days 2, 5, and 14. While in short term, decreased cell survival rate was observed at 1000 ng/ml and above, at long term no statistically significant difference was detected in different B concentrations applied. Slight decreases at the proliferation of the B-treated groups were determined on days 5 and 14 but one-way analysis of variance revealed that the difference was statistically insignificant. In mineralization assay, increased mineralized nodules were apparently observed in B treatment (1 and 10 ng/ml concentrations) groups. Based on quantitative RT-PCR results, remarkable regulation in favor of osteoblastic function for Collagen type I (COL I), Osteopontin (OPN), Bone Sialoprotein (BSP), Osteocalcin (OCN) and RunX2 mRNA expressions were observed in B treatment groups in comparison with untreated control groups. Increased BMP-4, -6 and -7 protein levels were detected at 0.1, 1, 10 and 100 ng/ml B concentrations. Results of the study suggest that at the molecular level B displays important roles on bone metabolism and may find novel usages at the regenerative medicine.
Hakki
Sema S
SS
Selcuk University, Faculty of Dentistry, Department of Periodontology, Konya, Turkey. sshakki@yahoo.com
Bozkurt
Buket S
BS
Hakki
Erdogan E
EE
D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D055417:Bone Morphogenetic Protein 5 / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D001895:Boron / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D024042:Collagen Type I / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D058574:Integrin-Binding Sialoprotein / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics; D053495:Osteopontin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C525810:Bmp4 protein, mouse; C525815:Bmp5 protein, mouse; D055415:Bone Morphogenetic Protein 4; D055417:Bone Morphogenetic Protein 5; D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; C525820:bmp7 protein, mouse; D015675:Osteocalcin; D053495:Osteopontin; D001895:Boron