The control of axillary meristem fate in the maize ramosa pathway.
Plant axillary meristems are composed of highly organized, self-renewing stem cells that produce indeterminate branches or terminate in differentiated structures, such as the flowers. These opposite fates, dictated by both genetic and environmental factors, determine interspecific differences in the architecture of plants. The Cys(2)-His(2) zinc-finger transcription factor RAMOSA1 (RA1) regulates the fate of most axillary meristems during the early development of maize inflorescences, the tassel and the ear, and has been implicated in the evolution of grass architecture. Mutations in RA1 or any other known members of the ramosa pathway, RAMOSA2 and RAMOSA3, generate highly branched inflorescences. Here, we report a genetic screen for the enhancement of maize inflorescence branching and the discovery of a new regulator of meristem fate: the RAMOSA1 ENHANCER LOCUS2 (REL2) gene. rel2 mutants dramatically increase the formation of long branches in ears of both ra1 and ra2 mutants. REL2 encodes a transcriptional co-repressor similar to the TOPLESS protein of Arabidopsis, which is known to maintain apical-basal polarity during embryogenesis. REL2 is capable of rescuing the embryonic defects of the Arabidopsis topless-1 mutant, suggesting that REL2 also functions as a transcriptional co-repressor throughout development. We show by genetic and molecular analyses that REL2 physically interacts with RA1, indicating that the REL2/RA1 transcriptional repressor complex antagonizes the formation of indeterminate branches during maize inflorescence development. Our results reveal a novel mechanism for the control of meristem fate and the architecture of plants.
Gallavotti
Andrea
A
Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093-0116, USA. agallavotti@ucsd.edu
Long
Jeff A
JA
Stanfield
Sharon
S
Yang
Xiang
X
Jackson
David
D
Vollbrecht
Erik
E
Schmidt
Robert J
RJ
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D019915:DNA Primase / Q000235:genetics; D004742:Enhancer Elements, Genetic; D017343:Genes, Plant; D006824:Hybridization, Genetic; D018519:Meristem / Q000254:growth & development / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics; D054730:Protein Interaction Domains and Motifs; D012097:Repressor Proteins / Q000235:genetics; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D016335:Zinc Fingers / Q000235:genetics
D029681:Arabidopsis Proteins; D019915:DNA Primase; D010940:Plant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C511291:At1g15750 protein, Arabidopsis