title: Mesophilic fermentation of renewable biomass: does hydraulic retention time regulate methanogen diversity?
abstract: The present long-term study (about 1,100 days) monitored the diversity of methanogens during the mesophilic, anaerobic digestion of beet silage. Six fermentor samples were analyzed by ribosomal RNA gene restriction analysis, fluorescence in situ hybridization, and fluorescence microscopy. Hydrogenotrophic methanogens dominated within the population in all samples analyzed. Multidimensional scaling revealed that a rapid decrease in hydraulic retention time resulted in increased species richness, which in turn led to slightly higher CH(4) yields.
authors.affiliation: Hamburg University of Applied Sciences, Research Centre of Lifetec Process Engineering, 21033 Hamburg-Bergedorf, Germany. niclas.krakat@haw-hamburg.de
authors.forename: Niclas
authors.initials: N
authors.lastname: Krakat
authors.forename: Stefan
authors.initials: S
authors.lastname: Schmidt
authors.forename: Paul
authors.initials: P
authors.lastname: Scherer
mesh_terms: D001483:Base Sequence; D027461:Beta vulgaris / Q000378:metabolism*; D044822:Biodiversity; D016000:Cluster Analysis; D019605:Euryarchaeota / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics*; D020459:Genes, rRNA / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D012336:RNA, Ribosomal, 16S