title: Cloning and distribution of Ca2+-activated K+ channels in lobster Panulirus interruptus.
abstract: Large conductance Ca(2+)-activated potassium (BK) channels play important roles in controlling neuronal excitability. We cloned the PISlo gene encoding BK channels from the spiny lobster, Panulirus interruptus. This gene shows 81-98% sequence identity to Slo genes previously found in other organisms. We isolated a number of splice variants of the PISlo cDNA within Panulirus interruptus nervous tissue. Sequence analysis indicated that there are at least seven alternative splice sites in PISlo, each with multiple alternative segments. Using immunohistochemistry, we found that the PISlo proteins are distributed in the synaptic neuropil, axon and soma of stomatogastric ganglion (STG) neurons.
authors.affiliation: Department of Neurobiology and Behavior, Cornell University, Ithaca,NY 14853, USA. qingouyang2000@gmail.com
authors.forename: Q
authors.initials: Q
authors.lastname: Ouyang
authors.forename: V
authors.initials: V
authors.lastname: Patel
authors.forename: J
authors.initials: J
authors.lastname: Vanderburgh
authors.forename: R M
authors.initials: RM
authors.lastname: Harris-Warrick
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D017952:Ganglia, Invertebrate / Q000378:metabolism; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D019581:Neuropil / Q000378:metabolism; D033521:Palinuridae / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D020033:Protein Isoforms