Subnuclear localization and intranuclear trafficking of transcription factors.
Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
Zaidi
Sayyed K
SK
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
Medina
Ricardo F
RF
Pockwinse
Shirwin M
SM
Bakshi
Rachit
R
Kota
Krishna P
KP
Ali
Syed A
SA
Young
Daniel W
DW
Nickerson
Jeffrey A
JA
Javed
Amjad
A
Montecino
Martin
M
van Wijnen
Andre J
AJ
Lian
Jane B
JB
Stein
Janet L
JL
Stein
Gary S
GS
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D019295:Computational Biology; D036681:Fluorescence Recovery After Photobleaching; D007382:Intermediate Filaments / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D042581:Intranuclear Space / Q000378:metabolism*; D008677:Metaphase; D008853:Microscopy; D015530:Nuclear Matrix / Q000378:metabolism; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
D014157:Transcription Factors