title: Cloning, purification, crystallization and preliminary crystallographic analysis of LsrR from Escherichia coli.
abstract: In Escherichia coli, the lsr operon is composed of six genes lsrACDBFG which regulate uptake and modification of the signalling molecule AI-2. LsrR is a repressor of the lsr operon and itself, which can bind phospho-AI-2 and be released from the promoter region of the operon and thus activate gene expression. LsrR fused with an HHHHHH sequence at the C-terminus was expressed, purified and crystallized in order to determine its structure and elucidate the molecular mechanism of repression. The crystal belonged to space group I222, with unit-cell parameters a=79.84, b=116.65, c=186.04 A, and was estimated to contain two protein molecules per asymmetric unit.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Liu
authors.forename: Minhao
authors.initials: M
authors.lastname: Wu
authors.forename: Demeng
authors.initials: D
authors.lastname: Sun
authors.forename: Jianye
authors.initials: J
authors.lastname: Zang
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D029968:Escherichia coli Proteins; C504601:LsrR protein, E coli; D012097:Repressor Proteins