Multilineage differentiation of dental pulp stem cells from green fluorescent protein transgenic mice.
OBJECTIVE
The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also observed during differentiation.


METHODS
DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR.


RESULTS
The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation.


CONCLUSIONS
As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo.
Grottkau
Brian E
BE
Department of Orthopaedic Surgery, Mass General Hospital for Children and the Pediatric Orthopaedic Laboratory for Tissue Engineering, Harvard Medical School, Boston, Massachusetts, USA.
Purudappa
P Prasad
PP
Lin
Yun-feng
YF
D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D000880:Anthraquinones; D001391:Azo Compounds; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis / Q000502:physiology; D004396:Coloring Agents; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D005819:Genetic Markers / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D046509:Tissue Culture Techniques; D014048:Tolonium Chloride
D000880:Anthraquinones; D001391:Azo Compounds; D004396:Coloring Agents; D003470:Culture Media; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D014048:Tolonium Chloride; C004468:Alizarin Red S; D012313:RNA; C011049:oil red O