Rapid screening of lectins for multivalency effects with a glycodendrimer microarray.
Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.
Parera Pera
Núria
N
Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht, The Netherlands.
Branderhorst
Hilbert M
HM
Kooij
Raymond
R
Maierhofer
Caroline
C
van der Kaaden
Marjolein
M
Liskamp
Rob M J
RM
Wittmann
Valentin
V
Ruijtenbeek
Rob
R
Pieters
Roland J
RJ
D002241:Carbohydrates / Q000737:chemistry; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D037102:Lectins / Q000032:analysis*; D046228:Microarray Analysis / Q000379:methods*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D002241:Carbohydrates; D050091:Dendrimers; D037102:Lectins