A 1-bp deletion in the gammaC-crystallin leads to dominant cataracts in mice.
To date around 140 genetic alleles have been identified as being responsible for mouse cataract pathology, including Crya, Cryb, Cryg, Maf, Pax6, Pitx3, Sox, Connexins, MIP, and Lim-2. We obtained a dominant cataract mouse model from a spontaneous mutation in the F1 hybrids of outbred strain ICR mice crossed to the inbred strain BALB/cJ mice. Heterozygous and homozygous mutants expressed a nuclear cataract in both eyes. In 8-day-old mice, histological analysis showed that polygon epithelial cells were in the equatorial region and cortex underneath, and vacuole and sponge-like degeneration were in the cortical area underneath the posterior lens capsule. The nucleus of the lens was a deeply stained pink, with the shorter fibers losing their normal arrangement. For the entire eye, there was a blank zone in the equatorial region in 8-day-old mice; however, there was a certain degree of atrophy in cornea tension and retina in the lens in 3-month-old mice. The lens had been serious damaged in the homozygous mutants. For mutation mapping, heterozygous carriers were mated to wild-type C3H/HeJ mice, and offspring (F1 generation) with cataracts were backcrossed to the wild-type C3H/HeJ mice again. N2 mice with cataracts were used for genotyping. Using genome-wide linkage analysis, the mutation was mapped to chromosome 1 and the Cryg gene cluster between two markers was confirmed as the candidate gene. After direct sequencing the cDNA of the Cryg gene cluster, a 1-bp deletion was found in exon 3 of the Crygc gene, leading to a stop codon at the 76th amino acid of exon 3 which results in production of a truncated protein in mutant mice (Leu160Stop). Bioinformatic analysis of the mutant gammaC-crystallin reveals that the COOH-terminal of the mutant protein deletes a beta-sheet, which affects the function of the lens proteins and leads to the development of cataracts.
Zhao
Liya
L
Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, China.
Li
Kai
K
Bao
Shimin
S
Zhou
Yuxun
Y
Liang
Yinming
Y
Zhao
Guoji
G
Chen
Ye
Y
Xiao
Junhua
J
D000818:Animals; D020137:Base Pair Mismatch / Q000502:physiology; D002386:Cataract / Q000235:genetics* / Q000473:pathology; D005260:Female; D005799:Genes, Dominant; D008040:Genetic Linkage; D007908:Lens, Crystalline / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008813:Mice, Inbred ICR; D008817:Mice, Mutant Strains; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000502:physiology; D038222:gamma-Crystallins / Q000235:genetics*
D038222:gamma-Crystallins; C554179:gammaC-crystallin protein, mouse