Study of synthesis of nano-hydroxyapatite using a silk fibroin template.
Nano-hydroxyapatite (HA) was directly synthesized on a silk fibroin (SF) template using the property of SF being soluble in a concentrated CaCl(2) solution as a HA source of calcium at pH 7.4 and room temperature. The microstructure and bonding state were investigated by x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry-thermogravimetry analysis (DSC-TG) and transmission electron microscopy (TEM). The results indicated that the HA crystals were poorly crystallized with a rod-like shape of 20-60 nm length and 10-20 nm diameter. Strong molecular interactions and chemical bonds might be present between SF and HA. There were other nucleation sites such as carbonyl (-C-O) and amine (-N-H-) groups on SF molecules besides the carboxyl (-COOH) and hydroxyl (-OH) groups previously reported. During the formation of HA, the coordination action between specific functional groups on SF and calcium ions (Ca(2+)) played an important role. The crystallinity of HA was improved and had an orientation growth along (0 0 2) at the presence of SF, resulting in a structure similar to natural bone. It was concluded that SF could regulate the structure and morphology of HA effectively.
Wang
Jing
J
Provincial Key Laboratory of Biomaterials, Jiamusi University, Jiamusi 154007, People's Republic of China. jmsdxwangjing@163.com
Yu
Feng
F
Qu
Lijie
L
Meng
Xiangcai
X
Wen
G
G
D018786:Bone Substitutes / Q000138:chemical synthesis*; D003460:Crystallization / Q000379:methods*; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D013499:Surface Properties
D018786:Bone Substitutes; D046911:Macromolecular Substances; C482288:fibroin, silkworm; D005349:Fibroins; D017886:Durapatite