title: Phase 1 of an in vitro neurotoxicological pre-validation trial.
abstract: In the absence of a formal in vitro alternative model for neurotoxicity testing, the CellTox Centre, in collaboration with the University of Salford, FRAME and the EEC, has set up an initial pre-validation trial of a three-tiered scheme that was originally proposed in 1989. Using a cell battery of neuroblastomas and primary neural cultures (Tier I) and primary astrocytes (Tier III) an initial set of 20 chemicals (out of a reference set of 40 chemicals) has been assessed for neurotoxicity using a multiple end point assay system. The methods used were relatively simple, rapid and inexpensive, and the chemical end points were easily quantified. The results of the pre-validation trial have enabled us to revise the first tier of the proposed tiered system for full micro-validation.
authors.affiliation: CellTox Centre, Division of Biosciences, University of Hertfordshire, College Lane, Hatfield, Herts. AL10 9AB, UK.
authors.forename: S P
authors.initials: SP
authors.lastname: Williams
authors.forename: J
authors.initials: J
authors.lastname: Davenport-Jones
authors.forename: C
authors.initials: C
authors.lastname: Egan
authors.forename: S
authors.initials: S
authors.lastname: O'Hare
authors.forename: M
authors.initials: M
authors.lastname: Cookson
authors.forename: R
authors.initials: R
authors.lastname: McClean
authors.forename: M J
authors.initials: MJ
authors.lastname: Garle
authors.forename: V
authors.initials: V
authors.lastname: Pentreath
authors.forename: C K
authors.initials: CK
authors.lastname: Atterwill