title: Determination of ginsenoside Rg3 in human plasma and urine by high performance liquid chromatography-tandem mass spectrometry.
abstract: Here we report a method capable of quantifying ginsenoside Rg3 in human plasma and urine. The method was validated over linear range of 2.5-1000.0ngmL(-1) for plasma and 2.0-20.0ngmL(-1) for urine using ginsenoside Rg1 as I.S. Compounds were extracted with ethyl acetate and analyzed by HPLC/MS/MS (API-4000 system equipped with ESI(-) interface and a C(18) column). The inter- and intra-day precision and accuracy of QC samples were <or=8.5% relative error and were <or=14.4% relative standard deviation for plasma; were <or=5.6% and <or=13.3% for urine. The Rg3 was stable after 24h at room temperature, 3 freeze/thaw cycles and 131 days at -30 degrees C. This method has been applied to pharmacokinetic study of ginsenoside Rg3 in human.
authors.affiliation: Clinical Pharmacology Research Centre, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Ji
authors.initials: J
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D006801:Humans; D016014:Linear Models; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D036145:Ginsenosides; C097367:ginsenoside Rg3