Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines.
In this study, the first micro-total analysis system (μ-TAS) for catecholamines (dopamine, epinephrine, and norepinephrine) analysis in which preconcentration, separation, and determination steps were integrated on a microchip was developed. Electrophoresis microchips in a variety of channel lengths and designs were produced in borofloat glass for the μ-TAS studies. Chambers for the preparation of monolithic disks were formed in the microchips at the intersection of the injection and separation channels. Vinyl phenylboronic acid-ethylene glycol dimethacrylate polymers were prepared as monolithic disks in these chambers with a depth of 0.05 mm and a diameter of 2.1 mm. The microchips could be used more than 50 times if mechanical problems such as plugging or fracturing did not occur. Adsorption and elution of catecholamines were realized electrokinetically, with catecholamines determined via laser-induced native fluorescence detection following elution and electrophoretic separation. The most promising results were obtained with 100 mM phosphate buffer (pH 2) for elution with 25% propanol added to the separation buffer (100 mM phosphate, pH 3).
Cakal
Cafer
C
The Institute for Graduate Studies in Science and Engineering, Bioengineering Subdivision, Hacettepe University, 06532 Beytepe, Ankara, Turkey.
Ferrance
Jerome P
JP
Landers
James P
JP
Caglar
Perihan
P
D002395:Catecholamines / Q000032:analysis*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration
D002395:Catecholamines