Direct molecular analysis of whole-body animal tissue sections by MALDI imaging mass spectrometry.
The determination of the localization of various compounds in a whole animal is valuable for many applications, including pharmaceutical absorption, distribution, metabolism, and excretion (ADME) studies and biomarker discovery. Imaging mass spectrometry is a powerful tool for localizing compounds of biological interest with molecular specificity and relatively high resolution. Utilizing imaging mass spectrometry for whole-body animal sections offers considerable analytical advantages compared to traditional methods, such as whole-body autoradiography, but the experiment is not straightforward. This chapter addresses the advantages and unique challenges that the application of imaging mass spectrometry to whole-body animal sections entails, including discussions of sample preparation, matrix application, signal normalization, and image generation. Lipid and protein images obtained from whole-body tissue sections of mouse pups are presented along with detailed protocols for the experiments.
Reyzer
Michelle L
ML
Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.
Chaurand
Pierre
P
Angel
Peggi M
PM
Caprioli
Richard M
RM
D000818:Animals; D000831:Animals, Newborn; D003952:Diagnostic Imaging / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D051379:Mice; D011506:Proteins / Q000737:chemistry; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D008055:Lipids; D011506:Proteins