Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted.
Sung
Li-Ying
LY
Institute of Biotechnology, National Taiwan University , Taipei 106, Taiwan.
Chang
Ching-Chien
CC
Amano
Tomokazu
T
Lin
Chih-Jen
CJ
Amano
Misa
M
Treaster
Stephen B
SB
Xu
Jie
J
Chang
Wei-Fang
WF
Nagy
Zsolt Peter
ZP
Yang
Xiangzhong
X
Tian
X Cindy
XC
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D019976:Cloning, Organism; D015925:Cryopreservation / Q000379:methods*; D046149:Embryo Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D010312:Parthenogenesis; D044968:Regenerative Medicine