The anionic (9-methyladenine)-(1-methylthymine) base pair solvated by formic acid. A computational and photoelectron spectroscopy study.
The photoelectron spectrum for (1-methylthymine)-(9-methyladenine)...(formic acid) (1MT-9MA...FA) anions with the maximum at ca. 1.87 eV was recorded with 2.54 eV photons and interpreted through the quantum-chemical modeling carried out at the B3LYP/6-31+G(d,p) level. The relative free energies of the anions and their calculated vertical detachment energies suggest that only seven anionic structures contribute to the observed PES signal. We demonstrate that electron binding to the (1MT-9MA...FA) complex can trigger intermolecular proton transfer from formic acid, leading to the strong stabilization of the resulting radical anion. The SOMO distribution indicates that an excess electron may localize not only on the pyrimidine but also on the purine moiety. The biological context of DNA-environment interactions concerning the formation of single-strand breaks induced by excess electrons has been briefly discussed.
Storoniak
Piotr
P
Department of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdańsk, Poland. pondros@chem.univ.gda.pl
Mazurkiewicz
Kamil
K
Haranczyk
Maciej
M
Gutowski
Maciej
M
Rak
Janusz
J
Eustis
Soren N
SN
Ko
Yeon Jae
YJ
Wang
Haopeng
H
Bowen
Kit H
KH
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D020029:Base Pairing; D005561:Formates / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011789:Quantum Theory; D012997:Solvents / Q000737:chemistry; D013816:Thermodynamics; D013941:Thymine / Q000031:analogs & derivatives* / Q000737:chemistry
D000838:Anions; D005561:Formates; D012997:Solvents; C030544:formic acid; C030630:1-methylthymine; C013115:9-methyladenine; D000225:Adenine; D013941:Thymine