Determination of ginsenoside Rg3 in human plasma and urine by high performance liquid chromatography-tandem mass spectrometry.
Here we report a method capable of quantifying ginsenoside Rg3 in human plasma and urine. The method was validated over linear range of 2.5-1000.0ngmL(-1) for plasma and 2.0-20.0ngmL(-1) for urine using ginsenoside Rg1 as I.S. Compounds were extracted with ethyl acetate and analyzed by HPLC/MS/MS (API-4000 system equipped with ESI(-) interface and a C(18) column). The inter- and intra-day precision and accuracy of QC samples were <or=8.5% relative error and were <or=14.4% relative standard deviation for plasma; were <or=5.6% and <or=13.3% for urine. The Rg3 was stable after 24h at room temperature, 3 freeze/thaw cycles and 131 days at -30 degrees C. This method has been applied to pharmacokinetic study of ginsenoside Rg3 in human.
Zhao
Qian
Q
Clinical Pharmacology Research Centre, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Zheng
Xin
X
Jiang
Ji
J
Zhou
Hui
H
Hu
Pei
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine; D006801:Humans; D016014:Linear Models; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D036145:Ginsenosides; C097367:ginsenoside Rg3