Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1.
To identify the putative interacting partners for Kiaa0319-like protein. KIAA0319-like, located near the dyslexia susceptibility locus, DYX8 in chromosome 1p34.3, has been suggested as a positional candidate for developmental dyslexia due to its homology with another gene, KIAA0319 which has been strongly established as a candidate gene for developmental dyslexia. Previous research has shown that a single marker, rs7523017 (P = 0.042) has been associated with developmental dyslexia by a Canadian group. There is little functional information about this gene and protein. In this article, we put forward further evidence that support Kiaa0319-like is a candidate for this disorder. A yeast-2-hybrid screen and co-immunopreciptiation assays were performed to find protein interacting partners of KIAA0319L. A human cortex immunohistochemistry assay was performed to show the colocalization of Kiaa0319-like and its specific interacting partner in cells. Nogo Receptor 1 (NgR1), an axon guidance receptor, was identified to have physical interactions with Kiaa0319-like protein. These two proteins interact predominantly in the cytoplasmic granules of cortical neurons in the human brain cortex. Based on this data, it can be concluded that Kiaa0319-like protein interacts with Nogo Receptor 1, supporting the idea that Kiaa0319-like protein participates in axon guidance.
Poon
Ming-Wai
MW
The Chinese University of Hong Kong, Shatin N.T., China.
Tsang
Wan-Hong
WH
Chan
Sun-On
SO
Li
Hiu-Ming
HM
Ng
Ho-Keung
HK
Waye
Mary Miu-Yee
MM
D001369:Axons / Q000378:metabolism* / Q000502:physiology*; D001706:Biopsy; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000473:pathology; D004410:Dyslexia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008954:Models, Biological; D009185:Myelin Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D000070816:Nogo Receptor 1; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*
D058851:GPI-Linked Proteins; C541251:KIAA0319L protein, human; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D000070816:Nogo Receptor 1; D009687:Nuclear Proteins; C486580:RTN4R protein, human; D011956:Receptors, Cell Surface