Nanoscale fabrication of a peptide layer in cell chip to detect effects of environmental toxins on HEK293 cells.
A cysteine-terminated C(RGD)(4) peptide film was fabricated on a gold electrode for improving the attachment of cells. The electrochemical signals of cyclic voltammogram from cells on a C(RGD)(4) deposited electrode was enhanced from 0.27 to 0.49 μA compared to a bare electrode. The developed cell-based sensor determined the effect of bisphenol-A (BPA) and dichlorodiphenyltrichloroethane (DTT) on the viability of HEK-293 cells by detecting decrease of reduction peaks (1.12-0.15 μA for BPA and 0.81-0.29 μA for DDT) after the treatment of environmental chemicals. This developed system can be a powerful tool for the monitoring of environmental toxicants.
Abdul Kafi
Md
M
Interdisciplinary Program of Integrated Biotechnology, #1 Shinsu-Dong Mapo-Gu, Seoul, 121-742, South Korea.
Kim
Tae-Hyung
TH
Yagati
Ajay Kumar
AK
Kim
Hyuncheol
H
Choi
Jeong-Woo
JW
D001559:Benzhydryl Compounds; D001681:Biological Assay / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D003634:DDT / Q000032:analysis / Q000633:toxicity*; D004560:Electricity; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis / Q000633:toxicity*; D057809:HEK293 Cells; D006801:Humans; D010636:Phenols / Q000032:analysis / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003634:DDT; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds