title: Development of gut microflora in obese and lean rats.
abstract: The influence was evaluated of post-weaning normal nutrition and over-nutrition upon the development of the intestinal microbiota, the alkaline phosphatase activity (AP) and occurrence of obesity in male Sprague-Dawley rats (from days 21 to 40 the control rats were submitted to ad libitum intake of a standard laboratory diet whereas overfed rats received the same diet supplemented with milk-based high fat liquid diet). The jejunal numbers of two dominant divisions of bacteria, i.e. Firmicutes (Lactobacillus/ Enterococcus--LAB) and the Bacteroidetes (Bacteroides/Prevotella--BAC), were determined using the fluorescent in situ hybridization (FISH) method, and the jejunal AP activity was assayed histochemically. On day 40, the overfed rats in comparison with control animals displayed increased adiposity accompanied by enhanced AP activity, abundance of LAB, lower amounts of BAC and, thereafter, higher LAB/BAC ratio (L/B). The numbers of LAB and L/B index positively correlated with body fat, energy intake and AP activity, whereas numbers of BAC showed an opposite tendency. These results revealed the significance of nutritional imprint upon the post-weaning development of intestinal microbial and functional axis and contribute to better understanding of their involvement in energy-balance control and in adverse and/or positive regulation of adiposity.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, 040 01, Kosice, Slovakia. sefcikz@saske.sk
authors.forename: Z
authors.initials: Z
authors.lastname: Sefcíková
authors.forename: V
authors.initials: V
authors.lastname: Kmet
authors.forename: D
authors.initials: D
authors.lastname: Bujnáková
authors.forename: L
authors.initials: L
authors.lastname: Racek
authors.forename: S
authors.initials: S
authors.lastname: Mozes
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D041963:Bacteroidetes / Q000145:classification* / Q000302:isolation & purification*; D044822:Biodiversity; D004032:Diet; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification; D006651:Histocytochemistry; D017404:In Situ Hybridization, Fluorescence; D007583:Jejunum / Q000201:enzymology / Q000382:microbiology*; D008297:Male; D009765:Obesity / Q000382:microbiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000469:Alkaline Phosphatase