title: Generation of cytotoxic T lymphocytes for immunotherapy of EBV-associated malignancies.
abstract: Current approaches for the treatment of tumours typically employ broad acting radiotherapeutic and chemotherapeutic approaches, which have led to high success rates but can be associated with unwanted side-effects. Cytotoxic T cell (CTL)-based immunotherapy offers an alternative approach that is designed to specifically target protein antigens expressed in malignant cells and is thus likely to limit any adverse side-effects. Defining tumour-specific antigens is therefore critical for the successful application of CTL-based therapy. Epstein-Barr virus (EBV)-associated malignancies offer an attractive target for CTL-based immunotherapy due to presence of virally encoded antigens in the malignant cells. Recent success in treating Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disorder (PTLD) using cytotoxic T cell (CTL)-based immunotherapy has led to interest in the development of CTL-based immunotherapy to treat other EBV-associated malignancies in which antigen expression patterns are well defined but limited to a restricted number of proteins.
authors.affiliation: Tumour Immunology Laboratory, Division of Infectious Diseases and Immunology, Australian Centre for Vaccine Development, Queensland Institute of Medical Research, Brisbane, Australia.
authors.forename: Corey
authors.initials: C
authors.lastname: Smith
authors.forename: Rajiv
authors.initials: R
authors.lastname: Khanna
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D002460:Cell Line; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D005822:Genetic Vectors / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000472:pathogenicity*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D008223:Lymphoma / Q000276:immunology / Q000628:therapy* / Q000821:virology*; D010835:Phytohemagglutinins; D011995:Recombination, Genetic; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D010835:Phytohemagglutinins; D007371:Interferon-gamma