Composition analysis and anti-hypoxia activity of polysaccharide from Brassica rapa L.
Crude water-soluble polysaccharides (BRP) were extracted from the root of Brassica rapa L. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-100 column, giving three major polysaccharide fractions termed BRP1-1, BRP2-1, BRP3-1. The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides (BRP1-1, BRP2-1, BRP3-1) were approximately 5.53×10(3) Da, 3.35×10(4) Da and 3.37×10(4) Da, respectively. Monosaccharide components analysis indicated that BRP1-1 was composed of arabinose and glucose in a molar ratio of 1.66:98.34. BRP2-1 was composed of arabinose, galactose and glucose in a molar ratio of 9.3:14.63:76.07. BRP3-1 was composed of arabinose, rhamnose, galactose and glucose in a molar ratio of 24.98:24.10:44.09:6.83. The evaluation of anti-hypoxia activity in vivo revealed that BRP is a novel potential anti-hypoxia agent.
Xie
Yue
Y
College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
Jiang
Siping
S
Su
Donghai
D
Pi
Ningning
N
Ma
Chao
C
Gao
Ping
P
D000208:Acute Disease; D000818:Animals; D002545:Brain Ischemia / Q000097:blood / Q000150:complications / Q000503:physiopathology; D029741:Brassica rapa / Q000737:chemistry*; D005591:Chemical Fractionation; D002845:Chromatography; D004906:Erythrocyte Count; D006439:Hemodynamics / Q000187:drug effects; D006454:Hemoglobins / Q000378:metabolism; D000860:Hypoxia / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D012977:Sodium Nitrite / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D016019:Survival Analysis; D013997:Time Factors; D018675:Toxicity Tests
D006454:Hemoglobins; D009005:Monosaccharides; D011134:Polysaccharides; D012977:Sodium Nitrite