title: Adsorption and bioactivity of tyrosine hydroxylase on gold surfaces and nanoparticles.
abstract: Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.
authors.affiliation: Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. Oyvind.Halskau@biomed.uib.no
authors.forename: Oyvind
authors.initials: O
authors.lastname: Halskau
authors.forename: Sondre
authors.initials: S
authors.lastname: Volden
authors.forename: Ana C
authors.initials: AC
authors.lastname: Calvo
authors.forename: Aurora
authors.initials: A
authors.lastname: Martínez
authors.forename: Wilhelm R
authors.initials: WR
authors.lastname: Glomm
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020033:Protein Isoforms; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D020033:Protein Isoforms; D011994:Recombinant Proteins; D006046:Gold; D014446:Tyrosine 3-Monooxygenase