Structural determinants for the stereoselective hydrolysis of chiral substrates by phosphotriesterase.
Wild-type phosphotriesterase (PTE) preferentially hydrolyzes the R(P) enantiomers of the nerve agents sarin (GB) and cyclosarin (GF) and their chromophoric analogues. The active site of PTE can be subdivided into three binding pockets that have been denoted as the small, large, and leaving group pockets based on high-resolution crystal structures. The sizes and shapes of these pockets dictate the substrate specificity and stereoselectivity for catalysis. Mutants of PTE that exhibit substantial changes in substrate specificity and the ability to differentiate between chiral substrates have been prepared. For example, the G60A mutant is stereoselective for the hydrolysis of the R(P) enantiomer of the chromophoric analogues of sarin and cyclosarin, whereas the H254G/H257W/L303T (GWT) mutant reverses the stereoselectivity for the enantiomers of these two compounds. Molecular dynamics simulations and high-resolution X-ray structures identified the correlations between structural changes in the active site and the experimentally determined kinetic parameters for substrate hydrolysis. New high-resolution structures were determined for the H257Y/L303T (YT), I106G/F132G/H257Y (GGY), and H254Q/H257F (QF) mutants of PTE. Molecular dynamics calculations were conducted using the S(P) and R(P) enantiomers of the analogues for sarin and cyclosarin for the wild-type PTE and the G60A, YT, GGY, QF, and GWT mutants. The experimental stereoselectivity correlated nicely with the difference in the computed angle of attack for the nucleophilic hydroxide relative to the phenolic leaving group of the substrate.
Tsai
Ping-Chuan
PC
Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
Fan
Yubo
Y
Kim
Jungwook
J
Yang
Lijiang
L
Almo
Steven C
SC
Gao
Yi Qin
YQ
Raushel
Frank M
FM
D002384:Catalysis; D002619:Chemical Warfare Agents / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D056004:Molecular Dynamics Simulation; D009943:Organophosphorus Compounds; D044345:Phosphoric Triester Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D012524:Sarin / Q000378:metabolism; D013237:Stereoisomerism; D013379:Substrate Specificity; D014965:X-Rays
D002619:Chemical Warfare Agents; D009943:Organophosphorus Compounds; D012524:Sarin; D044345:Phosphoric Triester Hydrolases; C053914:cyclohexyl methylphosphonofluoridate