In situ evaluation of chromium-DNA damage using a DNA-electrochemical biosensor.
The in situ evaluation of the direct interaction of chromium species with double-stranded DNA (dsDNA) was studied using differential pulse voltammetry at a glassy carbon electrode. The DNA damage was electrochemically detected following the changes in the oxidation peaks of guanosine and adenosine bases. The results obtained revealed the interaction with dsDNA of the Cr(IV) and Cr(V) reactive intermediates of Cr(III) oxidation by O(2) dissolved in the solution bound to dsDNA. This interaction leads to different modifications and causes oxidative damage in the B-DNA structure. Using polyhomonucleotides of guanine and adenine, it was shown that the interaction between reactive intermediates Cr(IV) and Cr(V)-DNA causes oxidative damage and preferentially takes place at guanine-rich segments, leading to the formation of 8-oxoguanine, the oxidation product of guanine residues and a biomarker of DNA oxidative damage. The interaction of Cr(VI) with dsDNA causes breaking of hydrogen bonds, conformational changes, and unfolding of the double helix, which enables easier access of other oxidative agents to interact with DNA, and the occurrence of oxidative damage to DNA.
Oliveira
S Carlos B
SC
Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, Portugal.
Oliveira-Brett
A M
AM
D015374:Biosensing Techniques / Q000379:methods*; D002857:Chromium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004249:DNA Damage; D055664:Electrochemical Techniques / Q000379:methods*; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction
D002857:Chromium; D004247:DNA