Cellular origin of platelet-derived microparticles in vivo.
BACKGROUND
Microparticles (MP), presumably of platelet origin, are the most abundant microparticles in blood. To which extent such MP may also directly originate from megakaryocytes, however, is unknown. During hematopoietic stem cell transplantation, patients undergo total body irradiation which leads to an irreversible destruction of hematopoiesis.


METHODS
We studied the levels of "platelet-derived" MP (PMP) in 13 patients before and after total body irradiation with 12 Gy (4 Gy for 3 days, dose rate 4.5 cGy/min). PMP were isolated and double-stained with annexin V and anti-CD61. In 6 patients, we additionally analyzed MP exposing P-selectin or CD63.


RESULTS
PMP rapidly declined upon total body irradiation, which was 2.4-fold faster than platelet disappearance. In contrast, the kinetics of MP exposing P-selectin or CD63 was comparable to platelets.


CONCLUSIONS
Since CD61-positive MP disappear faster than platelets or MP exposing P-selectin or CD63, our data indicate that MP exposing P-selectin or CD63 are likely to originate from platelets, whereas at least a major fraction of CD61-exposing MP is likely to originate from megakaryocytes in vivo.
Rank
Andreas
A
Medizinische Klinik III-Großhadern, Klinikum der Ludwig Maximilians-Universität München, Marchioninistraße 15, 81377 München, Germany. andreas.rank@med.uni-muenchen.de
Nieuwland
Rienk
R
Delker
Ruth
R
Köhler
Anton
A
Toth
Bettina
B
Pihusch
Verena
V
Wilkowski
Ralf
R
Pihusch
Rudolf
R
D000328:Adult; D001792:Blood Platelets / Q000378:metabolism* / Q000528:radiation effects*; D055252:Cell-Derived Microparticles / Q000378:metabolism* / Q000528:radiation effects*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D014916:Whole-Body Irradiation / Q000009:adverse effects*; D055815:Young Adult