Identification and characterization of prohibitin as a receptor protein mediating DENV-2 entry into insect cells.
Dengue is transmitted primarily by mosquitoes of the Aedes genus. Despite a number of studies, no insect dengue virus receptor protein has been clearly identified and characterized. Using a number of separation methodologies and virus overlay protein binding assays we identified a 35kDa protein that segregated with susceptibility to dengue serotype 2 (DENV-2) infection in two mosquito species and two mosquito cell lines. Mass spectroscopy identified the protein to be prohibitin, a strongly conserved and ubiquitously expressed protein in eukaryotic cells. Antibody mediated inhibition of infection and siRNA mediated knockdown of prohibitin expression significantly reduced infection levels and subsequent virus production in both Aedes aegypti and Aedes albopictus cell lines. Confocal microscopy showed a significant degree of intracellular colocalization between prohibitin and DENV-2 E protein, and coimmunoprecipitation confirmed that prohibitin interacts with dengue E. Prohibitin is the first characterized insect cell expressed dengue virus receptor protein.
Kuadkitkan
Atichat
A
Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Thailand.
Wikan
Nitwara
N
Fongsaran
Chanida
C
Smith
Duncan R
DR
D000330:Aedes / Q000235:genetics / Q000502:physiology / Q000821:virology; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D000091402:Prohibitins; D034741:RNA, Small Interfering / Q000235:genetics; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000502:physiology; D053586:Virus Internalization; D014779:Virus Replication
D019476:Insect Proteins; D000091402:Prohibitins; D034741:RNA, Small Interfering; D011991:Receptors, Virus; D012097:Repressor Proteins; D014759:Viral Envelope Proteins