Acquisition, preparation, and functional assessment of human NK cells for adoptive immunotherapy.
Human natural killer (NK) cells, a subset of peripheral blood lymphocytes that lack a T- or B-cell receptor, play a crucial role in the innate immune response to viruses and malignant cells. NK cells differentiate infected or malignant cells from normal cells by a complex balance between activating and inhibitory receptor-ligand interactions. Unlike T cells, NK cells do not proliferate in vitro in response to simple crosslinking of a single activating receptor. While many methods to study T-cell function and phenotype can also be applied to NK cells, this chapter addresses methods that are unique to the preparation and assessment of human NK cells for immunotherapy.
Lee
Dean A
DA
Division of Pediatrics, Cell Therapy Section, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
Verneris
Michael R
MR
Campana
Dario
D
D015550:Cell Degranulation; D049109:Cell Proliferation; D002469:Cell Separation; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology