The synergy of 6-O-sulfation and N- or 3-O-sulfation of chitosan is required for efficient inhibition of P-selectin-mediated human melanoma A375 cell adhesion.
We prepared chitosan sulfated derivatives to address the common structural requirement of the sulfate pattern to block P-selectin-mediated tumor cell adhesion. Our results indicate that 6-O-sulfation of chitosan is indispensable for inhibition of P-selectin binding to human melanoma A375 cells. Furthermore, additional N-sulfation or 3-O-sulfation dramatically enhanced the inhibitory activity of 6-O-sulfated chitosan, suggesting that efficient anti-P-selectin adhesion activity of sulfated saccharides requires the synergy of 6-O-sulation and N- or 3-O-sulfation in glucosamine units.
Wang
Ruifei
R
Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Renmin Street, Chanchun, PR China.
Huang
Jinfeng
J
Wei
Min
M
Zeng
Xianlu
X
D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008545:Melanoma / Q000473:pathology*; D010100:Oxygen / Q000737:chemistry*; D019007:P-Selectin / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry*
D019007:P-Selectin; D013431:Sulfates; D048271:Chitosan; D010100:Oxygen