title: Simian immunodeficiency virus-specific CD8+ T cells recognize Vpr- and Rev-derived epitopes early after infection.
abstract: The kinetics of CD8(+) T cell epitope presentation contribute to the antiviral efficacy of these cells yet remain poorly defined. Here, we demonstrate presentation of virion-derived Vpr peptide epitopes early after viral penetration and prior to presentation of Vif-derived epitopes, which required de novo Vif synthesis. Two Rev epitopes exhibited differential presentation kinetics, with one Rev epitope presented within 1 h of infection. We also demonstrate that cytolytic activity mirrors the recognition kinetics of infected cells. These studies show for the first time that Vpr- and Rev-specific CD8(+) T cells recognize and kill simian immunodeficiency virus (SIV)-infected CD4(+) T cells early after SIV infection.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI 53711, USA. jbsacha@wisc.edu
authors.forename: Jonah B
authors.initials: JB
authors.lastname: Sacha
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Buechler
authors.forename: Laura P
authors.initials: LP
authors.lastname: Newman
authors.forename: Jason
authors.initials: J
authors.lastname: Reed
authors.forename: Lyle T
authors.initials: LT
authors.lastname: Wallace
authors.forename: John T
authors.initials: JT
authors.lastname: Loffredo
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Wilson
authors.forename: David I
authors.initials: DI
authors.lastname: Watkins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology*; D003602:Cytotoxicity, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D015694:Gene Products, rev / Q000235:genetics / Q000276:immunology*; D016336:Gene Products, vpr / Q000235:genetics / Q000276:immunology*; D054884:Host-Pathogen Interactions / Q000276:immunology; D066298:In Vitro Techniques; D007700:Kinetics; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000201:enzymology / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology
chemical_list: D018984:Epitopes, T-Lymphocyte; D015694:Gene Products, rev; D016336:Gene Products, vpr