High-resolution local imaging of temperature in dielectrophoretic platforms.
The use of dielectrophoretic forces is crucially tied to the knowledge of Joule heating within a fluid, since the use of planar microelectrodes creates a temperature gradient within which the particle of interest is manipulated. Mapping temperature with sufficient spatial resolution within a dielectrophoretic trap is recognized to be of high importance. Herein, we demonstrate local temperature measurements in the vicinity of a trapped micrometer-size particle using confocal fluorescence spectroscopy. Such measurements are shown to provide a novel calibration tool for screening temperature-mediated processes with high resolution.
Gielen
Fabrice
F
Institute of Biomedical Engineering, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom.
Pereira
Fiona
F
Demello
Andrew J
AJ
Edel
Joshua B
JB
D005456:Fluorescent Dyes / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D005456:Fluorescent Dyes; D012235:Rhodamines; C029773:rhodamine B