title: Different expression of Sox9 and Runx2 between chondrosarcoma and dedifferentiated chondrosarcoma cell line.
abstract: Dedifferentiated chondrosarcoma (DDCS), a highly malignant variant of chondrosarcoma (CCS), is characterized by high-grade sarcoma adjacent to low-grade chondroid tumor. Owing to its complicated composition, the histogenesis of this tumor remains controversial. Earlier, we carried out DNA microarray analysis using chondrosarcomatous tissues, and found that Sox9 and runt-related transcription factor 2 (Runx2) were differentially expressed in CCS compared with DDCS. Here, we analyzed Sox9, Runx2, Col2a1, and Col1a1 in NDCS-1 (DDCS) and SW1353 (CCS) cell lines using reverse transcriptase-polymerase chain reaction, western blot, and immunocytochemistry. The results showed high expression of Runx2 and Col1a1, and low expression of Sox9 and Col2a1 in NDCS-1 cells. In SW1353 cells, however, gene expressions were reversed. These findings provide evidence that Sox9 and Runx2 are involved in the occurrence and development of DDCS.
authors.affiliation: Musculoskeletal Tumor Center, Peking University People's Hospital, Beijing, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tang
authors.forename: Xinchang
authors.initials: X
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Tingting
authors.initials: T
authors.lastname: Ren
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Fulong
authors.initials: F
authors.lastname: Zhao
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tang
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism*; D054337:Cell Dedifferentiation; D045744:Cell Line, Tumor; D002813:Chondrosarcoma / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D055758:SOX9 Transcription Factor / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C499177:COL2A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II; D050659:Core Binding Factor Alpha 1 Subunit; D009363:Neoplasm Proteins; D055758:SOX9 Transcription Factor