title: Platelet-derived growth factor-BB release profile in gingival crevicular fluid after use of marginal periosteal pedicle graft as an autogenous guided tissue membrane to treat localized intrabony defects.
abstract: BACKGROUND
  The aim of this study is to evaluate levels of platelet-derived growth factor-BB (PDGF-BB) in gingival crevicular fluid (GCF) during the early stages of healing for sites treated by marginal periosteal pedicle (MPP) graft as an autogenous guided tissue membrane compared to that of the control open flap debridement (OFD).
  
  
  METHODS
  Fifteen non-smoking patients (13 males and 2 females) with severe chronic periodontitis participated in this prospective, controlled, masked trial. Each subject contributed matched pairs of 2- or 3-walled intrabony interproximal defects in premolar or molar teeth. Interproximal contralateral defects were randomly assigned to either the MPP group 1 or control OFD group 2. GCF samples were collected at 1, 3, 7, 14, and 30 days after surgery. PDGF-BB in the GCF samples was measured using a human PDGF-BB enzyme-linked immunosorbent assay kit.
  
  
  RESULTS
  In both MPP and OFD, PDGF-BB concentrations peaked in the samples obtained during the early postoperative days (days 2 and 3) and decreased sharply in the samples obtained 7, 14, and 30 days post-surgery.
  
  
  CONCLUSIONS
  Periosteal coverage of periodontal defects is not associated with a significant increase in PDGF-BB levels.
authors.affiliation: Department of Periodontology, Faculty of Dental Medicine, Al Azhar University, Cairo, Egypt. hgamal@hotmail.com
authors.forename: Ahmed Yousef
authors.initials: AY
authors.lastname: Gamal
authors.forename: Ossama Sayed Ahmed
authors.initials: OS
authors.lastname: El-Shal
authors.forename: Mahmoud Mohammed
authors.initials: MM
authors.lastname: El-Aasara
authors.forename: Eman Mohammed
authors.initials: EM
authors.lastname: Fakhry
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000628:therapy; D000077214:Becaplermin; D001861:Bone Regeneration / Q000502:physiology*; D055113:Chronic Periodontitis / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D008297:Male; D016555:Matched-Pair Analysis; D008875:Middle Aged; D010521:Periosteum / Q000637:transplantation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011446:Prospective Studies; D020574:Proto-Oncogene Proteins c-sis; D016037:Single-Blind Method; D013524:Surgical Flaps
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin