Glutathione content, glutathione transferase activity and lipid peroxidation in acrylamide-treated neuroblastoma N1E 115 cells.
Acrylamide is a well known neurotoxic compound that produces central and peripheral distal axonopathy. Degenerative changes of this type can be induced in the neuroblastoma cell line C 1300, clone N1E 115 and have been extensively studied in our laboratory particularly with regard to the interference by acrylamide with cellular metabolism. In the present study the mechanism of acrylamide-induced neurite degeneration in N1E 115 cells is elucidated further. Acrylamide concentrations were selected that were not cytotoxic but caused an increasingly severe neurite degeneration. The rate of protein synthesis was decreased in a concentration-dependent manner in response to acrylamide exposure (0-2.5 mm). Detoxification of acrylamide in vivo occurs mainly through conjugation with glutathione, (GSH) both non-enzymatically and enzymatically by glutathione S-transferases (GST). Cells grown in the presence of acrylamide showed a concentration-dependent decrease in GSH content. At the highest acrylamide concentration tested this was accompanied by an increased GST activity. Despite the reduced level of GSH and possible impaired protection of the plasma membrane against oxidative stress no elevated level of lipid peroxidation could be observed in the acrylamide-treated cells.
Odland
L
L
Department of Neurochemistry and Neurotoxicology, Stockholm University, S-106 91 Stockholm, Sweden.
Romert
L
L
Clemedson
C
C
Walum
E
E