IgE, COX-2, and IL-4 are expressed by DEHP through p38 MAPK and suppressed by plant glycoprotein (75 kDa) in ICR mice.
The aim of the present study was to evaluate the inhibitory effect of glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on di(2-ethylhexyl) phthalate (DEHP)-induced allergic inflammatory response in mice. We evaluated the activity of β-hexosaminidase, expression of cyclooxygenase (COX)-2, p38 mitogen-activated protein kinase (MAPK), and activator protein (AP)-1, and production of immunoglobulin (Ig)E and interleukin (IL)-4 in DEHP-treated RBL-2H3 cells and ICR mice. Our results revealed that the CTB glycoprotein inhibited the activity of β-hexosaminidase and production of IgE and IL-4 in serum from DEHP-treated mice. We also found that the CTB glycoprotein reduced arachidonic acid release, COX-2 expression, and AP-1 transcriptional activation through p38 MAPK phosphorylation in DEHP-treated RBL-2H3 cells. The activation of AP-1 was completely blocked by treatment with p38 MAPK inhibitor (SKF86002). The results from these experiments indicate that CTB glycoprotein effectively protects against the allergic inflammation response, mainly through downregulation of MAPK/AP-1 in the mast cell degranulation stage. In conclusion, we suggest that the CTB glycoprotein may be one component of health supplements for the prevention of allergic inflammation.
Oh
Phil-Sun
PS
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju, 500-757, South Korea.
Lim
Kye-Taek
KT
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000097:blood; D004051:Diethylhexyl Phthalate / Q000633:toxicity; D005260:Female; D006967:Hypersensitivity / Q000209:etiology / Q000378:metabolism* / Q000517:prevention & control; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D029586:Moraceae / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D018808:Transcription Factor AP-1 / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000097:blood; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D010940:Plant Proteins; D018808:Transcription Factor AP-1; D015847:Interleukin-4; D016718:Arachidonic Acid; D007073:Immunoglobulin E; D004051:Diethylhexyl Phthalate; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases; D001619:beta-N-Acetylhexosaminidases