Polarimetry-based method to extract geometry-independent metrics of tissue anisotropy.
Recently, we have used polarimetry as a method for assessing the linear retardance of infarcted myocardium. While linear retardance reflects tissue anisotropy, experimental geometry has a confounding effect due to dependence of the linear retardance on the orientation of the sample with respect to the probing beam. Here, polarimetry imaging of an 8mm diameter birefringent polystyrene sphere of known anisotropy axis was used to test a dual-projection method by which the anisotropy axis and its true magnitude can be reconstructed, thus eliminating the confounding effect of anisotropy axis orientation. Feasibility is demonstrated in ex-vivo tissue imaging.
Wallenburg
Marika A
MA
Ontario Cancer Institute, University Health Network, and Department of Medical Biophysics,University of Toronto, Toronto, Ontario, Canada M5G 2M9.
Wood
Michael F G
MF
Ghosh
Nirmalya
N
Vitkin
I Alex
IA
D000465:Algorithms; D000818:Animals; D016880:Anisotropy; D001718:Birefringence; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008027:Light; D015233:Models, Statistical; D009206:Myocardium / Q000473:pathology*; D055095:Optics and Photonics; D011137:Polystyrenes / Q000737:chemistry; D057109:Scanning Laser Polarimetry / Q000379:methods*; D013552:Swine
D011137:Polystyrenes