title: Small interfering RNA (siRNA) against the survivin gene increases apoptosis in a canine melanoma cell line.
abstract: The effect of down-regulation of survivin expression by small interfering RNA (siRNA) against the canine survivin gene on apoptosis was investigated by transfecting MCM-N1 (a canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine survivin gene induced an increase in the rate of apoptotic cells and a decrease in the number of viable cells. We also measured sequence-specific down-regulation of survivin expression by reverse transcription-PCR and western blot analysis. The siRNA directed against survivin reduced both mRNA and protein expression in MCM-N1 cells. These findings suggest the importance of survivin in canine melanoma tumors for inducing apoptosis, and reinforce the possibility of using survivin as a putative therapeutic target in canine malignant melanoma tumor.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Moriyama
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Maruyama
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D008545:Melanoma / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering