The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.
The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.
Love
James
J
New York Structural Biology Center, New York, 10027, USA.
Mancia
Filippo
F
Shapiro
Lawrence
L
Punta
Marco
M
Rost
Burkhard
B
Girvin
Mark
M
Wang
Da-Neng
DN
Zhou
Ming
M
Hunt
John F
JF
Szyperski
Thomas
T
Gouaux
Eric
E
MacKinnon
Roderick
R
McDermott
Ann
A
Honig
Barry
B
Inouye
Masayori
M
Montelione
Gaetano
G
Hendrickson
Wayne A
WA
D002478:Cells, Cultured; D019295:Computational Biology; D018360:Crystallography, X-Ray; D016678:Genome; D023281:Genomics / Q000379:methods*; D008565:Membrane Proteins / Q000235:genetics*; D009518:New York; D017433:Protein Structure, Secondary
D008565:Membrane Proteins