title: [Expression characteristics of hypoxia inducible factor-1a and its clinical values in hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the dynamic expression of hypoxia inducible factor-1alpha (HIF-1alpha) and its clinical values in hepatocellular carcinoma (HCC).
  
  
  METHODS
  The dynamic changes of liver pathology, HIF-1alpha transcription and expression were observed through the hepatoma model. The self-control specimens from 35 human HCC patients were collected and the expression, cellular distribution, and clinicopathological features of HIF-1alpha and its gene was analyzed by immunohistochemistry, western blotting and nested- PCR, respectively.
  
  
  RESULTS
  Both levels of hepatic HIF-1alpha and HIF-1alpha mRNA expression increased during the HCC development course. The incidence of HIF-1alpha and the ratio of HIF-1alpha to beta-actin was 0% and 0.16+/-0.02 in the control rats, 77.8% and 0.29+/-0.04 in the denatured rats, 88.9% and 0.52+/-0.03 in the precancerous rats, and 100% and 0.84+/-0.02 in the cancerous rats respectively, with significant difference between the control group and any of the experimental groups (P = 0.000). The positive HIF-1alpha was brown and granule-like and mainly presented in cytoplasm and few in nucleus. The incidence of HIF-1alpha was 80% (28/35) in HCC and 100% (35/35) in its surrounding tissues. The clinical pathological features indicated HIF-1alpha expression associated with tumor size and differentiation degree the of HCC. No correlation was found between HIF-1alpha and tumor numbers or positive-HBsAg.
  
  
  CONCLUSIONS
  HIF-1alpha expression is associated with occurrence and development of HCC, and is perhaps a target molecule for HCC therapy.
authors.affiliation: Affiliated Hospital of Nantong University, Nantong 226001, China.
authors.forename: Zhi-zhen
authors.initials: ZZ
authors.lastname: Dong
authors.forename: Deng-fu
authors.initials: DF
authors.lastname: Yao
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Li
authors.forename: Yue-ming
authors.initials: YM
authors.lastname: Li
authors.forename: Bo-jun
authors.initials: BJ
authors.lastname: Tai
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Qiu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Ning-hua
authors.initials: NH
authors.lastname: Yao
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Sai
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger