Annual variation of Microcystis genotypes and their potential toxicity in water and sediment from a eutrophic reservoir.
The relative genetic diversity of microcystin-producing Microcystis in the water and sediment of the Daechung Reservoir, Korea, was investigated over an entire year, including the cyanobacterial bloom season. The cells of potentially toxic Microcystis strains containing mcyJ genotypes and cells containing the genus-specific cpcBA gene were quantified by a real-time PCR. The ratio of cells with mcyJ genotypes to the total Microcystis population in the water body was the highest (68.3%) in August when the cyanobacterial bloom reached its peak and the microcystin concentration in the water began to increase. A denaturing gradient gel electrophoresis profile analysis of the mcyJ genotypes performed to monitor any changes in the toxic Microcystis population showed the appearance of new genotypes and the disappearance of existing genotypes in the reservoir water collected during the summer months, when compared with the profile for the samples collected in spring and autumn. However, very little change was observed over the course of the year as regards the population diversity of the sediment samples.
Kim
Song-Gun
SG
Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Republic of Korea.
Joung
Seung-Hyun
SH
Ahn
Chi-Yong
CY
Ko
So-Ra
SR
Boo
Sung Min
SM
Oh
Hee-Mock
HM
D044822:Biodiversity; D004269:DNA, Bacterial / Q000302:isolation & purification; D058645:Denaturing Gradient Gel Electrophoresis; D005068:Eutrophication; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D052998:Microcystins / Q000032:analysis; D046931:Microcystis / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D056910:Republic of Korea; D012621:Seasons; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D052998:Microcystins