title: Btbd7 regulates epithelial cell dynamics and branching morphogenesis.
abstract: During embryonic development, many organs form by extensive branching of epithelia through the formation of clefts and buds. In cleft formation, buds are delineated by the conversion of epithelial cell-cell adhesions to cell-matrix adhesions, but the mechanisms of cleft formation are not clear. We have identified Btbd7 as a dynamic regulator of branching morphogenesis. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation through its highly focal expression leading to local regulation of Snail2 (Slug), E-cadherin, and epithelial cell motility. Inhibition experiments show that Btbd7 is required for branching of embryonic mammalian salivary glands and lungs. Hence, Btbd7 is a regulatory gene that promotes epithelial tissue remodeling and formation of branched organs.
authors.affiliation: Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-4370, USA.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Onodera
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Sakai
authors.forename: Jeff Chi-feng
authors.initials: JC
authors.lastname: Hsu
authors.forename: Kazue
authors.initials: K
authors.lastname: Matsumoto
authors.forename: John A
authors.initials: JA
authors.lastname: Chiorini
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D004285:Dogs; D004847:Epithelial Cells / Q000502:physiology*; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005809:Genes, Regulator; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008954:Models, Biological; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009687:Nuclear Proteins; D009924:Organ Culture Techniques; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D034741:RNA, Small Interfering; D012469:Salivary Glands / Q000196:embryology* / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D013363:Submandibular Gland / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C551480:Btbd7 protein, mouse; D015820:Cadherins; D005353:Fibronectins; D009687:Nuclear Proteins; D011506:Proteins; D034741:RNA, Small Interfering; C000604429:Snai1 protein, mouse; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors