Forsythoside a inhibits the avian infectious bronchitis virus in cell culture.
Forsythoside A is a polyphenolic constituent of the fruits of Forsythia suspensa Vahl. which is widely used as an antiinflammatory agent in traditional Chinese medicine. In the present study, the effects of forsythoside A on cell infection by avian infectious bronchitis virus were assessed. A real-time fluorescence quantitative PCR assay was used to determine mRNA content of IBV N gene. The pretreatment of cells with forsythoside A, adding forsythoside A post infection of cells, and treatment of virus with forsythoside A were analysed. The inhibitory effect of forsythoside A was confirmed by infecting primary chicken embryo kidney cells. Infected cells were inhibited by forsythoside A treatment. The data indicated that forsythoside A has the potential to prevent IBV infection in vitro. Copyright © 2010 John Wiley & Sons, Ltd.
Li
Huawei
H
Laboratory of Veterinary Medicine, Department of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Wu
Jufu
J
Zhang
Zhongwen
Z
Ma
Yuanyuan
Y
Liao
Fangfang
F
Zhang
Yu
Y
Wu
Guojuan
G
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D018352:Coronavirus Infections / Q000188:drug therapy / Q000517:prevention & control; D031657:Forsythia / Q000737:chemistry; D006027:Glycosides / Q000494:pharmacology*; D001351:Infectious bronchitis virus / Q000187:drug effects* / Q000472:pathogenicity; D007668:Kidney / Q000166:cytology / Q000821:virology; D019590:Nucleocapsid Proteins / Q000235:genetics; D014779:Virus Replication
D000998:Antiviral Agents; D006027:Glycosides; D019590:Nucleocapsid Proteins; C058049:forsythiaside