Modulation of the oligomerization of myelin proteolipid protein by transmembrane helix interaction motifs.
Proteolipid protein (PLP) is a highly hydrophobic 276-residue integral membrane protein that constitutes more than 50% of the total protein in central nervous system myelin. Previous studies have shown that this protein exists in myelin as an oligomer rather than as a monomer, and mutations in PLP that lead to neurological disorders such as Pelizaeus-Merzbacher disease and spastic paraplegia type 2 have been reported to affect its normal oligomerization. Here we employ peptide-based and in vivo approaches to examine the role of the TM domain in the formation of PLP quaternary structure through homo-oligomeric helix-helix interactions. Focusing on the TM4 alpha-helix (sequence (239)FIAAFVGAAATLVSLLTFMIAATY(262)), the site of several disease-causing point mutations that involve putative small residue helix-helix interaction motifs in the TM4 sequence, we used SDS-PAGE, fluorescence resonance energy transfer, size-exclusion chromatography, and TOXCAT assays in an Escherichia coli membrane to show that the PLP TM4 helix readily assembles into varying oligomeric states. In addition, through targeted studies of the PLP TM4 alpha-helix with point mutations that selectively eliminate these small residue motifs via substitution of Gly, Ala, or Ser residues with Ile residues, we describe a potential mechanism through which disease-causing point mutations can lead to aberrant PLP assembly. The overall results suggest that TM segments in misfolded PLP monomers that expose and/or create surface-exposed helix-helix interaction sites that are normally masked may have consequences for disease.
Ng
Derek P
DP
Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Deber
Charles M
CM
D020816:Amino Acid Motifs / Q000502:physiology; D002850:Chromatography, Gel; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D031541:Fluorescence Resonance Energy Transfer; D008823:Micelles; D008954:Models, Biological; D009154:Mutation; D018991:Myelin Proteolipid Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization / Q000502:physiology; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D008823:Micelles; D018991:Myelin Proteolipid Protein