Accelerating high quality bioanalytical LC/MS/MS assays using fused-core columns.
High quality, ultra-fast bioanalytical LC/MS/MS methods were developed using short columns packed with fused-core particles and high (1.0-3.0 mL/min) flow rates. For more than two years, at flow rates up to 3.0 mL/min, using 0.33 min non-ballistic gradients, these methods were shown to provide comparable or better performance than slower assays for accuracy, precision, sensitivity, specificity, and ruggedness, and met all criteria required by the bioanalytical regulatory guidance.
Badman
Ethan R
ER
Hoffmann-La Roche Inc., Non-Clinical Safety, 340 Kingsland St., Nutley, NJ 07110, USA. ethan.badman@roche.com
Beardsley
Richard L
RL
Liang
Zhenmin
Z
Bansal
Surendra
S
D002853:Chromatography, Liquid / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000379:methods*; D010455:Peptides / Q000032:analysis; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D045424:Complex Mixtures; D010455:Peptides