[Effect of Mortierlla isabellina delta5-desaturases gene on arachidonic acid synthesis].
OBJECTIVE
To study the relation between mRNA expression of delta5-desaturase gene and arachidonic acid production.


METHODS
Using fluorescence quantitative PCR, we determined the mRNA expression levels of delta5-desaturase gene of five Mortierlla isabellina strains cultured for the same time and strain YZ-124 cultured for different time. Simultaneously, we measured the contents of arachidonic acid by gas chromatography.


RESULTS
The results showed that delta5-desaturase gene mRNA expression level of distinct strains was different. Expression level of the original strain As3. 3410 was the lowest, whereas that of the mutant strain YZ-124 was the highest. The mRNA expression of delta5-desaturases gene decreased gradually with fermentation time.


CONCLUSIONS
According to arachidonic acid yield, mRNA expression quantity of delta5-desaturase gene presented a positive correlation with arachidonic acid content in cultured oil.
Yu
Changqing
C
College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China. spxypjb@126.com
Yang
Jian
J
Yao
Di
D
Li
Lina
L
D016718:Arachidonic Acid / Q000096:biosynthesis*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D016718:Arachidonic Acid; D044943:Fatty Acid Desaturases