title: Kinetic study for a stress testing of l,l-ethylenedicysteine by ultra-performance liquid chromatography/tandem mass spectrometry analysis.
abstract: This study proposed a stress testing to study oxidative stability and estimate the potential shelf-life of l,l-ethylenedicysteine (l,l-EC) under normal storage temperature condition (20-25 degrees C). l,l-EC was detected as a function of time at four different temperatures by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). The degradation of l,l-EC followed the first order kinetics, and the temperature-dependent kinetics was well described by the linear Arrhenius equation. The activation energy (E(a)) was calculated, and the shelf-life at 25 and 4 degrees C was predicted. The results are useful for the proper storage and quality evaluation of l,l-EC.
authors.affiliation: Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Sun
authors.forename: Jinping
authors.initials: J
authors.lastname: Qiao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Hongwen
authors.initials: H
authors.lastname: Qiao
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhong
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C109063:ethylene dicysteine; D003545:Cysteine