title: Induction of vascular endothelial growth factor receptor-3 mRNA in glial cells following focal cerebral ischemia in rats.
abstract: To identify whether vascular endothelial growth factor receptor (VEGFR)-3, a receptor for VEGF-C and VEGF-D, is involved in pathophysiology of stroke, we investigated the spatiotemporal regulation of VEGFR-3 mRNA after transient focal cerebral ischemia. Most of the increase in VEGFR-3 expression in the ischemic core could be attributed to brain macrophages, whereas VEGFR-3 in the peri-infarct penumbra region was predominantly expressed in reactive astrocytes. A subpopulation of VEGFR-3-expressing brain macrophages was positive for NG2 proteoglycan and showed proliferative activity. In addition, in vitro model of stroke revealed no significant induction of VEGFR-3 in activated microglial cells, indicating that infiltrating exogenous macrophages expressed VEGFR-3 after focal ischemia. These data suggest that VEGFR-3 may be involved in the glial reaction and possibly in the recruitment of monocytic macrophages during ischemic insults.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Shin
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Jae-Youn
authors.initials: JY
authors.lastname: Choi
authors.forename: Yun
authors.initials: Y
authors.lastname: Hou
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D053331:Ectodysplasins / Q000378:metabolism; D007839:Functional Laterality; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000378:metabolism; D000860:Hypoxia / Q000473:pathology; D066298:In Vitro Techniques; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000235:genetics* / Q000378:metabolism; D053675:Versicans / Q000378:metabolism
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D053331:Ectodysplasins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D012333:RNA, Messenger; C508324:Vcan protein, rat; D053675:Versicans; D040321:Vascular Endothelial Growth Factor Receptor-3; D010751:Phosphopyruvate Hydratase; D005947:Glucose