Microscope spectrometer for light scattering investigations.
We describe a setup including a microscope to study volumes of a few mum(3) by static and dynamic light scattering (DLS) in a backscattering configuration. Light scattered by individual objects of micrometric size can be analyzed in the 400-800?nm spectral range. This setup can also be employed to study both diluted and concentrated colloidal solutions by DLS measurements. For diluted solutions we found evidence of the fluctuations of the number of particles in a confocal volume. We discuss their contribution to the autocorrelation function of the scattered intensity measured as a function of time.
Barbara
Aude
A
Institut Néel, CNRS and University Joseph Fourier, BP 166, 38042 Grenoble Cedex 9, France. aude.barbara@grenoble.cnrs.fr
Lopez-Rios
Tomas
T
Dumont
Sylvain
S
Gay
Frédéric
F
Quémerais
Pascal
P
D000818:Animals; D003102:Colloids / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods; D015233:Models, Statistical; D016011:Normal Distribution; D055095:Optics and Photonics; D010316:Particle Size; D012542:Scattering, Radiation; D013997:Time Factors
D003102:Colloids