Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model.
We examined the change of protein tyrosine kinases (PTKs) expression levels in colonic epithelial cells isolated from mice in which colitis was induced by oxazolone administration, using the monoclonal antibody YK34, which cross-reacts with a wide variety of PTKs. We identified focal adhesion kinase (FAK) and found the expression level increased due to the induction of colitis. Furthermore, we found that there was a positive correlation between FAK expression and the severity of colitis. Also, FAK expression localized in the colonic epithelium but not in the lamina propria, implying FAK functions in epithelial cells during colitis formation and/or wound repairing.
Ma
Yanju
Y
Department of Biochemistry, Asahikawa Medical University, Asahikawa, Japan.
Semba
Shingo
S
Maemoto
Atsuo
A
Takeuchi
Masayuki
M
Kameshita
Isamu
I
Ishida
Atsuhiko
A
Kato
Sanae
S
Katoh
Tsuyoshi
T
Liu
Yunpeng
Y
Taniguchi
Takanobu
T
D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000201:enzymology*; D004195:Disease Models, Animal; D051417:Focal Adhesion Kinase 1 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000494:pharmacology
D010081:Oxazolone; D051417:Focal Adhesion Kinase 1; C496051:Ptk2 protein, mouse