[The effect of vitamin E on renal ischemia/reperfusion injury in young and aged rats].
OBJECTIVE
To investigate the effect of vitamin E on renal ischemia/reperfusion injury (RI/RI) in young and aged rats.


METHODS
The model of (RI/RI) was induced by bilateral clamping the renal artery and vein for 45 min followed by reperfusion. The contents of BUN, Scr, MDA, SOD, NO and iNOS were measured. Proteins of HSP70 were observed by immunohistochemistry. Flow cytometer was used to estimate the apoptosis rate of renal cortex cells.


RESULTS
After ischemia/reperfusion injury, the contents of BUN, Scr and iNOS were increased. Compared with young ischemia/reperfusion group, the contents of MDA were higher and the contents of SOD were lower in aged ischemia/reperfusion group. The expression of HSP70, the contents of NO and the apoptosis rate of renal cortex cells were higher in aged ischemia/reperfusion group than that in control group. Vitamin E significantly decreased the contents of BUN, Scr, MDA and iNOS, and increased the contents of NO and SOD after RI/RI. The expression of HSP70 was increased in both VE groups than that in both I/R groups. The apoptosis rate of renal cortex cells was less in both VE groups than that in both I/R groups.


CONCLUSIONS
Vitamins E may up-regulate the expression of HSP70, increase the contents of SOD and NO, and enhance an ability of clear free radicals, which may contributes to decreasing renal ischemia/reperfusion injury in young and aged rats.
Zhang
Wei
W
Department of Physiology, North Coal Medical College, Tangshan 063000, China.
Wang
Yan-ling
YL
Wang
Hui-juan
HJ
Miao
Zhi-hui
ZH
Xia
Xiao-hong
XH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007511:Ischemia / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D014810:Vitamin E; D009569:Nitric Oxide; D013482:Superoxide Dismutase