title: No association of polymorphisms in the suppressor of cytokine signaling (SOCS)-3 with rheumatoid arthritis in the Chinese Han population.
abstract: Suppressor of cytokine signaling (SOCS)-3 is a key negative regulator of cytokine signaling that inhibits the JAK/STAT signal transduction pathway; there are reports describing its role in attenuating arthritis through SOCS-3 overexpression. We examined the relationship between polymorphisms in the coding sequence and promoter region of SOCS-3 and rheumatoid arthritis (RA) in a Chinese Han population. Two single-nucleotide polymorphisms in the SOCS-3 5' region: -1044 C>A within the promoter region and rs12953258 (-920 C>A) in the 5'UTR (exon 2) of SOCS-3 were studied by restriction fragment length polymorphism analysis and tetra-ARMS-PCR in 100 RA patients and 100 healthy adults. The prevalence of the homozygous genotype -1044 CC was 100% in both RA and control groups. The heterozygous genotype (-920 C>A) was present in 89% of RA and in 82% of the control group, which is significantly different from the distribution in Western people. There was no transmission disequilibrium between these two SNPs (r(2) = 0.000). We did not detect significant differences in allele or genotype frequencies for either of these SNPs between the RA group and controls (P > 0.05). There was no association between rheumatoid factor and SOCS-3 SNP rs12953258 (P = 0.258). We conclude that SOCS-3 polymorphism is not a genetic risk factor for RA in Chinese patients.
authors.affiliation: Department of Rheumatology, Qilu Hospital, Shandong University, Jinan, PR China.
authors.forename: L P
authors.initials: LP
authors.lastname: Sun
authors.forename: X L
authors.initials: XL
authors.lastname: Ma
authors.forename: H X
authors.initials: HX
authors.lastname: Liu
authors.forename: Y S
authors.initials: YS
authors.lastname: Wang
authors.forename: X F
authors.initials: XF
authors.lastname: Li
mesh_terms: D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*
chemical_list: C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins