An economical single-sided antibody incubation method for Western blotting.
A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.
Pan
Chuanying
C
College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
Lan
Xianyong
X
Chen
Hong
H
Bishop
Colin E
CE
D000906:Antibodies / Q000032:analysis*; D015153:Blotting, Western / Q000191:economics* / Q000379:methods*; D012680:Sensitivity and Specificity
D000906:Antibodies