title: Identification of a novel iron regulated basic helix-loop-helix protein involved in Fe homeostasis in Oryza sativa.
abstract: BACKGROUND
  Iron (Fe) is the most limiting micronutrient element for crop production in alkaline soils. A number of transcription factors involved in regulating Fe uptake from soil and transport in plants have been identified. Analysis of transcriptome data from Oryza sativa grown under limiting Fe conditions reveals that transcript abundances of several genes encoding transcription factors are altered by Fe availability. These transcription factors are putative regulators of Fe deficiency responses.
  
  
  RESULTS
  Transcript abundance of one nuclear located basic helix-loop-helix family transcription factor, OsIRO3, is up-regulated from 25- to 90-fold under Fe deficiency in both root and shoot respectively. The expression of OsIRO3 is specifically induced by Fe deficiency, and not by other micronutrient deficiencies. Transgenic rice plants over-expressing OsIRO3 were hypersensitive to Fe deficiency, indicating that the Fe deficiency response was compromised. Furthermore, the Fe concentration in shoots of transgenic rice plants over-expressing OsIRO3 was less than that in wild-type plants. Analysis of the transcript abundances of genes normally induced by Fe deficiency in OsIRO3 over-expressing plants indicated their induction was markedly suppressed.
  
  
  CONCLUSIONS
  A novel Fe regulated bHLH transcription factor (OsIRO3) that plays an important role for Fe homeostasis in rice was identified. The inhibitory effect of OsIRO3 over-expression on Fe deficiency response gene expression combined with hypersensitivity of OsIRO3 over-expression lines to low Fe suggest that OsIRO3 is a negative regulator of the Fe deficiency response in rice.
authors.affiliation: Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Luqing
authors.initials: L
authors.lastname: Zheng
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Ying
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: James
authors.initials: J
authors.lastname: Whelan
authors.forename: Huixia
authors.initials: H
authors.lastname: Shou
mesh_terms: D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis / Q000235:genetics*; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D008969:Molecular Sequence Data; D012275:Oryza / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D007501:Iron; D010940:Plant Proteins