Practical preparation of D-galactosyl-beta1-->4-L-rhamnose employing the combined action of phosphorylases.
D-Galactosyl-beta1-->4-L-rhamnose (GalRha) was produced enzymatically from 1.1 M sucrose and 1.0 M L-rhamnose by the concomitant actions of four enzymes (sucrose phosphorylase, UDP-glucose-hexose 1-phosphate uridylyltransferase, UDP-glucose 4-epimerase, and D-galactosyl-beta1-->4-L-rhamnose phosphorylase) in the presence of 1.0 mM UDP-glucose and 30 mM inorganic phosphate. The accumulation of GalRha in 1 liter of the reaction mixture reached 230 g (the reaction yield was 71% from L-rhamnose). Sucrose and fructose in the reaction mixture were removed by yeast treatment, but isolation of GalRha by crystallization after yeast treatment was unsuccessful. Finally, 49 g of GalRha was isolated from part of the reaction mixture with yeast treatment by gel-filtration chromatography.
Nakajima
Masahiro
M
National Food Research Institute, National Agriculture and Food Research Organization., Kannondai, Tsukuba, Ibaraki, Japan. mkitaoka@affrc.go.jp
Nishimoto
Mamoru
M
Kitaoka
Motomitsu
M
D001644:Bifidobacterium / Q000201:enzymology; D003013:Clostridium / Q000201:enzymology; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D006005:Phosphorylases / Q000378:metabolism*; D012210:Rhamnose / Q000096:biosynthesis* / Q000737:chemistry
D004187:Disaccharides; C554195:galactosylrhamnose; D006005:Phosphorylases; D012210:Rhamnose