Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model.
A prospective, randomized study was performed in an ovine model to compare the efficacy of an anorganic bovine-derived hydroxyapatite matrix combined with a synthetic 15 amino acid residue (ABM/P-15) in facilitating lumbar interbody fusion when compared with autogenous bone harvested from the iliac crest. P-15 is a biomimetic to the cell-binding site of Type-I collagen for bone-forming cells. When combined with ABM, it creates the necessary scaffold to initiate cell invasion, binding, and subsequent osteogenesis. In this study, six adult ewes underwent anterior-lateral interbody fusion at L3/L4 and L4/L5 using PEEK interbody rings filled with autogenous bone at one level and ABM/P-15 at the other level and no additional instrumentation. Clinical CT scans were obtained at 3 and 6 months; micro-CT scans and histomorphometry analyses were performed after euthanization at 6 months. Clinical CT scan analysis showed that all autograft and ABM/P-15 treated levels had radiographically fused outside of the rings at the 3-month study time point. Although the clinical CT scans of the autograft treatment group showed significantly better fusion within the PEEK rings than ABM/P-15 at 3 months, micro-CT scans, clinical CT scans, and histomorphometric analyses showed there were no statistical differences between the two treatment groups at 6 months. Thus, ABM/P-15 was as successful as autogenous bone graft in producing lumbar spinal fusion in an ovine model, and it should be further evaluated in clinical studies.
Sherman
Blake P
BP
Department of Orthopaedics, The Spine Center, University of Colorado Denver, Mail Stop B202, 12631 E 17th Ave, Aurora, CO 80045, USA.
Lindley
Emily M
EM
Turner
A Simon
AS
Seim
Howard B
HB
Benedict
James
J
Burger
Evalina L
EL
Patel
Vikas V
VV
D000818:Animals; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen / Q000627:therapeutic use*; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000601:surgery*; D010446:Peptide Fragments / Q000627:therapeutic use*; D011446:Prospective Studies; D011859:Radiography; D011897:Random Allocation; D012756:Sheep; D013123:Spinal Fusion / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods
D018786:Bone Substitutes; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen