Surface-enhanced localized surface plasmon resonance biosensing of avian influenza DNA hybridization using subwavelength metallic nanoarrays.
We demonstrated enhanced localized surface plasmon resonance (SPR) biosensing based on subwavelength gold nanoarrays built on a thin gold film. Arrays of nanogratings (1D) and nanoholes (2D) with a period of 200 nm were fabricated by electron-beam lithography and used for the detection of avian influenza DNA hybridization. Experimental results showed that both nanoarrays provided significant sensitivity improvement and, especially, 1D nanogratings exhibited higher SPR signal amplification compared with 2D nanohole arrays. The sensitivity enhancement is associated with changes in surface-limited reaction area and strong interactions between bound molecules and localized plasmon fields. Our approach is expected to improve both the sensitivity and sensing resolution and can be applicable to label-free detection of DNA without amplification by polymerase chain reaction.
Kim
Shin Ae
SA
School of Electrical Engineering and Computer Science, Seoul National University, Seoul, Korea.
Byun
Kyung Min
KM
Kim
Kyujung
K
Jang
Seong Min
SM
Jang
Sung Min
SM
Ma
Kyungjae
K
Oh
Youngjin
Y
Kim
Donghyun
D
Kim
Sung Guk
SG
Shuler
Michael L
ML
Kim
Sung June
SJ
D015374:Biosensing Techniques / Q000379:methods*; D004279:DNA, Viral / Q000737:chemistry*; D006046:Gold; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*
D004279:DNA, Viral; D006046:Gold