Reversible conformational change of cytochrome c at a modified gold electrode in methanol.
Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.
Crilly
Suzanne
S
Materials and Surface Science Institute, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.
Magner
Edmond
E
D000818:Animals; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D000432:Methanol / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects
D004800:Enzymes, Immobilized; D006046:Gold; D045304:Cytochromes c; D000432:Methanol