Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) terpolymers using a chimeric PHA synthase in recombinant Ralstonia eutropha and Pseudomonas putida.
Recombinant strains of Ralstonia eutropha and Pseudomonas putida harboring a chimeric polyhydroxyalkanoate (PHA) synthase, which consisted of PHA synthases of Aeromonas caviae and R. eutropha, produced 3-hydroxybutyrate (3HB)-based PHA copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87-97 mol % 3HB), indicating that the chimeric PHA synthase possesses desirable substrate specificity leading to the production of 3HB-rich copolymers.
Sun
Jian
J
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan.
Shozui
Fumi
F
Yamada
Miwa
M
Matsumoto
Ken'ichiro
K
Takase
Kazuma
K
Taguchi
Seiichi
S
D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020118:Cupriavidus necator / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D016958:Pseudomonas putida / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D000217:Acyltransferases; D004274:DNA, Recombinant; D011091:Polyesters; D011108:Polymers; D011993:Recombinant Fusion Proteins; C554196:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate); D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase