Cryopreservation of the human ovarian tissue induces the expression of Fas system in morphologically normal primordial follicles.
The aim of this study was to investigate whether the Fas system was involved in cryopreservation process of the human ovarian tissue. Human ovarian cortical tissues were cryopreserved using slow freezing method. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to investigate apoptosis of primordial follicle. Fas, Fas ligand and active caspase-3 expression were detected by means of immunohistochemistry. The results showed that immunostaining for Fas, Fas ligand and active caspase-3 were not detected in morphologically normal primordial follicles in fresh ovarian tissue. After cryopreservation, Fas, Fas ligand and active caspase-3 immunostaining were present in morphologically normal primordial follicles. The study showed that cryopreservation of the human ovarian tissue induced the expression of Fas system in morphologically normal primordial follicles.
Xiao
Zhun
Z
Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, ChengDu 610041, China.
Wang
Yan
Y
Li
Lei
L
Li
Shang-Wei
SW
D000328:Adult; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D015925:Cryopreservation; D053222:Fas Ligand Protein / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D010053:Ovary; D055815:Young Adult; D019014:fas Receptor / Q000378:metabolism*
C491901:FAS protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor; D053148:Caspase 3