The polypeptide core of Microcin E492 stably associates with the mannose permease and interferes with mannose metabolism.
Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. The two known physiological activities of the mannose permease were assayed in cells co-expressing MceA and MceB. Under these conditions, growth on mannose as the sole carbon source is prevented; this was not observed in cells expressing only MceB. In contrast, susceptibility to bacteriophage λ infection was not affected.
Biéler
Sylvain
S
Department of Pathology and Immunology, University of Geneva, 1 Rue Michel-Servet, 1211 Geneva 4, Switzerland.
Silva
Filo
F
Belin
Dominique
D
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D000898:Antibiosis; D001426:Bacterial Proteins / Q000378:metabolism; D001430:Bacteriocins / Q000737:chemistry* / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D050296:Microbial Viability; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D008565:Membrane Proteins; C020411:microcin; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase; D008358:Mannose