An efficient protocol for VZV BAC-based mutagenesis.
Varicella-zoster virus (VZV) causes both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large 125-kb DNA genome, encoding 70 unique open reading frames (ORFs). The genetic study of VZV has been hindered by the large size of viral genome, and thus the functions of the majority of these ORFs remain unclear. Recently, an efficient protocol has been developed based on a luciferase-containing VZV bacteria artificial chromosome (BAC) system to rapidly isolate and study VZV ORF deletion mutants.
Zhang
Zhen
Z
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey - New Jersey Medical School, Newark, NJ, USA.
Huang
Ying
Y
Zhu
Hua
H
D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial; D018274:Electroporation; D014645:Herpesvirus 3, Human / Q000235:genetics*; D006801:Humans; D016296:Mutagenesis; D016366:Open Reading Frames; D011995:Recombination, Genetic