Anti-complement activity of essential oils from red and black rice bran.
The volatile essential oils from red and black rice bran were obtained by hydrodistillation using a clevenger-type apparatus, and the components of that oil were analyzed by capillary gas chromatography-mass spectroscopy (GC-MS). The present study involved characterizing the chemical compositions, their amounts and the anti-complement activities of red and black rice bran. The red rice bran essential oils yield was 0.031%, and GC-MS analysis revealed that its major constituents were (E)-β-ocimene (3.12%), nonanal (11.32%), (2E, 4E)-decadienal (2.54%), myristic acid (41.32%), geranyactone (2.41%) and methyl oleate (2.46%). The black rice bran essential oils yield was 0.053%, and GC-MS analysis revealed that its major constituents were nonanal (8.31%), acrylic acid (3.21%), 2-hydroxy-6-methylbenzaldehyde (2.81%), pelargonic acid (4.21%) and myrisitc acid (28.07%). The essential oils showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 246 ppm (red rice bran) and 193 ppm (black rice bran). Also, myristic acid, nonanal, (E)-β-ocimene and pelargonic acid were tested against complement system. Pelargonic acid was shown to moderate activity (50% inhibitory concentration = 132 μM).
Chung
Ill-Min
IM
Department of Applied Life Science, Kon Kuk University, Seoul, South Korea.
Yeo
Min-A
MA
Kim
Sun-Jin
SJ
Moon
Hyung-In
HI
D003167:Complement Activation / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012275:Oryza / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D000073879:Rice Bran Oil; D012639:Seeds / Q000737:chemistry*
D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D000073879:Rice Bran Oil