PEGylated iminodiacetic acid zinc complex stabilizes cationic RNA-bearing nanoparticles.
Self-assembling nanoparticles comprising cationic polymers are of interest for the delivery of oligonucleotide-based therapeutics. Unfortunately, exposure of the nanoparticle cationic surface to plasma and plasma proteins compromises particle stability and circulating half-life. Herein, we report that improved nanoparticle stability can be achieved through temporary grafting of PEG to the nanoparticle surface. Grafting is induced through zinc complexation between PEG-IDA and the exposed polyhistidylated polylysine (H-K) cationic polymer of pre-formed nanoparticles.
Levy
Daniel E
DE
Intradigm Corporation, 3350 West Bayshore Road, Suite 100, Palo Alto, CA 94303, USA. del345@gmail.com
Ding
Zhongli
Z
Hu
Chiwei
C
Zalipsky
Samuel
S
D007098:Imino Acids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D015032:Zinc / Q000737:chemistry*
D007098:Imino Acids; D034741:RNA, Small Interfering; C094451:poly(ethylene glycol-iminodiacetic acid); D011107:Polylysine; D011092:Polyethylene Glycols; D015032:Zinc