No evidence for XMRV association in pediatric idiopathic diseases in France.
Retroviruses have been linked to a variety of diseases such as neoplastic and immunodeficiency disorders and neurologic and respiratory diseases. Recently, a novel infectious human retrovirus, the xenotropic murine leukemia virus-related virus (XMRV), has been identified in cohorts of patients with either a familial type of prostate cancer or chronic fatigue syndrome. The apparent unrelatedness of these diseases raised the question of the potential involvement of XMRV in other diseases.Here, we investigated the presence of XMRV in a selection of pediatric idiopathic infectious diseases with symptoms that are suggestive of a retroviral infection, as well as in children with respiratory diseases and in adult patients with spondyloarthritis (SpA). Using a XMRV env-nested PCR, we screened 72 DNA samples obtained from 62 children hospitalized in the Montpellier university hospital (France) for hematological, neurological or inflammatory pathologies, 80 DNA samples from nasopharyngeal aspirates from children with respiratory diseases and 19 DNA samples from SpA. None of the samples tested was positive for XMRV or MLV-like env sequences, indicating that XMRV is not involved in these pathologies.
Jeziorski
Eric
E
Institut de Génétique Moléculaire de Montpellier UMR 5535 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
Foulongne
Vincent
V
Ludwig
Catherine
C
Louhaem
Djamel
D
Chiocchia
Gilles
G
Segondy
Michel
M
Rodière
Michel
M
Sitbon
Marc
M
Courgnaud
Valérie
V
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005602:France / Q000453:epidemiology; D017988:Gammaretrovirus / Q000235:genetics / Q000302:isolation & purification*; D006402:Hematologic Diseases / Q000821:virology; D006801:Humans; D007223:Infant; D009305:Nasopharynx / Q000821:virology; D009422:Nervous System Diseases / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000821:virology; D012192:Retroviridae Infections / Q000453:epidemiology* / Q000821:virology*; D013166:Spondylitis / Q000821:virology