Comparative study of adherence of oral Candida albicans isolates from HIV sero-positive individuals and HIV sero-negative individuals to human buccal epithelial cells.
OBJECTIVE
Candida albicans occurs as a commensal of the gastrointestinal tract. Under predisposing conditions, candida can produce a broad array of infections. HIV seropositive individuals show increased oral colonization compared to the HIV seronegative healthy individuals. C. albicans shows a variety of pathogenic factors. We have studied one such factor here; the adherence property of C. albicans isolated from HIV seropositive individuals and HIV seronegative to Human Buccal Epithelial Cells (HBEC) of normal healthy individuals.


METHODS
Concentrated oral rinse specimen were collected from 50 healthy volunteers (control group) and 25 HIV positive individuals (test group) and used for isolation of C. albicans. Adherence assay was done using C. albicans isolates from both groups on HBEC collected from HIV sero-negative, normal individuals. The adherence assay method described by Kimura and Pearsall was used with minor modification.


METHODS
The results of Adhesion assay were subjected to statistical analysis using student "t" test.


RESULTS
C. albicans isolated from both the groups were tested for their adherence property to normal HBEC. The isolates from test group showed more adherence to HBEC compared to those of the control group, with average rate of adherence being 56.6%. The control group showed average adherence rate of 29.1%. This was statistically significant with p value equal to 0.05.


CONCLUSIONS
C. albicans from HIV infected individuals showed significant rise in degree of adhesion to the buccal epithelial cells than the isolates from healthy controls, suggesting the enhancement of virulence factors such as adherence in the presence of predisposing condition.
Jain
Pavithra A
PA
Department of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad 580 009, Karnataka, India.
Veerabhadrudu
K
K
Kulkarni
R D
RD
Ajantha
G S
GS
Shubhada
C
C
Amruthkishan
U
U
D002176:Candida albicans / Q000472:pathogenicity*; D002180:Candidiasis, Oral / Q000382:microbiology; D002448:Cell Adhesion; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology*; D015658:HIV Infections / Q000150:complications; D006801:Humans; D009055:Mouth / Q000382:microbiology; D009061:Mouth Mucosa / Q000166:cytology*