Tandem mass spectrometric methods for phospholipid analysis from brain tissue.
We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS( n )) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly from tissue sections is described.
Garrett
Timothy J
TJ
GCRC Core Laboratory, Department of Medicine, University of Florida, Gainesville, FL, USA.
Yost
Richard A
RA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010743:Phospholipids