Response of Desulfovibrio vulgaris Hildenborough to hydrogen peroxide: enzymatic and transcriptional analyses.
We studied the effect of hydrogen peroxide (H(2)O(2)) stress on the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. In a lactate/sulfate medium, growth was affected from 0.1 mM H(2)O(2) and totally inhibited at 0.7 mM. Surprisingly, transcript analyses revealed that the PerR regulon exhibited opposite regulation in the presence of 0.1 and 0.3 mM H(2)O(2). The variations in peroxidase- and superoxide dismutase-specific activities in the cell-free extracts of H(2)O(2)-stressed cultures were related to changes in the corresponding transcript abundance. Our data suggest that sod, sor, ngr and tpx genes, in addition to the PerR regulon, belong to the H(2)O(2) stimulon.
Brioukhanov
Andrei L
AL
Laboratoire Interactions et Modulateurs de Réponses, IFR88, CNRS, Marseille, France.
Durand
Marie-Claire
MC
Dolla
Alain
A
Aubert
Corinne
C
D001426:Bacterial Proteins / Q000378:metabolism; D016969:Desulfovibrio vulgaris / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D001426:Bacterial Proteins; D016877:Oxidants; D012097:Repressor Proteins; C122138:peroxide repressor proteins; D006861:Hydrogen Peroxide; D009195:Peroxidase; D013482:Superoxide Dismutase