title: Electrochemical and spectral study on the effects of Al(III) and nano-Al13 species on glutamate dehydrogenase activity.
abstract: A functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE) was used to study the effects of aluminum species on glutamate dehydrogenase (GLDH) activity by monitoring amperometric i-t curve for the oxidation of the enzymatically generated NADH. The conformational changes of the coenzyme nicotinamide adenine dinucleotide (NAD(+)) induced by Al(III) and nanometer-sized tridecameric aluminum polycation (nano-Al(13)) were investigated by the fluorescence technique. The results showed that the electrochemical method may be a potential tool to investigate the activity of enzymes in the biological system. It may also be useful in studying the effects of nano-sized aluminum compounds on biomolecules in order to discuss their safety to the environment and human.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Cai
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Yang
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D000535:Aluminum / Q000737:chemistry* / Q000494:pharmacology; D017607:Aluminum Compounds / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D055162:Biocatalysis / Q000187:drug effects; D002417:Cattle; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005969:Glutamate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry; D010084:Oxidation-Reduction; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000535:Aluminum; D017607:Aluminum Compounds; D005969:Glutamate Dehydrogenase; D018698:Glutamic Acid; D009243:NAD; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers