title: Effect of acrylamide on cerebellar astrocyte proliferation in vitro.
abstract: Cerebellar astrocyte cultures derived from 7-day-old rats were exposed to acrylamide. Acrylamide dose-dependently inhibited the proliferation of astrocytes, 50% inhibition occurring at about 0.52 mm. Acrylamide reduced [(3)H]thymidine incorporation into DNA, but did not affect it when cytosine arabinoside was present. The main cause of the inhibition of astrocyte proliferation may be the suppression of mitosis. Interleukin-1, one of the factors that produce astrocyte proliferation after brain injury, increased [(3)H]thymidine incorporation into DNA in astrocyte cultures. Acrylamide was found to abolish this proliferative effect of interleukin-1 completely. It is suggested that acrylamide may influence the astrocytic response to brain injury and the regenerative process.
authors.affiliation: Department of Hygiene, School of Medicine, Kanazawa University, 13-1, Takara-machi, Kanazawa 920, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Tanii
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto