title: Fine mapping QMi-C11 a major QTL controlling root-knot nematodes resistance in Upland cotton.
abstract: The identification and utilization of a high-level of host plant resistance is the most effective and economical approach to control root-knot nematode (Meloidogyne incognita). In an earlier study, we identified a major quantitative trait locus (QTL) for resistance to root-knot nematode in the M-120 RNR Upland cotton line (Gossypium hirsutum L.) of the Auburn 623 RNR source. The QTL is located in a 12.9-cM interval flanked by the two SSR markers CIR069 and CIR316 on the distal segment of chromosome 11. To construct a fine map around the target region, a bulked segregation analysis was performed using two DNA pools consisting of five individuals, with each being homozygous for the two parental alleles. From a survey of 1,152 AFLP primer combinations, 9 AFLP markers closely linked to the target region were identified. By screening an additional 1,221 F(2) individuals developed from the initial mapping population, the Mi-C11 locus was delimited to a 3.6-cM interval flanked by the SSR marker CIR069 and the AFLP marker E14M27-375. These results further elucidate the genetic fine structure of the Mi-C11 locus and provide the basis for map-based isolation of the nematode resistance gene in M-120 RNR.
authors.affiliation: Department of Crop and Soil Sciences, University of Georgia, Tifton, GA 31793, USA.
authors.forename: Xinlian
authors.initials: X
authors.lastname: Shen
authors.forename: Yajun
authors.initials: Y
authors.lastname: He
authors.forename: Edward L
authors.initials: EL
authors.lastname: Lubbers
authors.forename: Richard F
authors.initials: RF
authors.lastname: Davis
authors.forename: Robert L
authors.initials: RL
authors.lastname: Nichols
authors.forename: Peng W
authors.initials: PW
authors.lastname: Chee
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers; D003368:Gossypium / Q000235:genetics* / Q000276:immunology / Q000469:parasitology; D007113:Immunity, Innate / Q000235:genetics*; D009348:Nematoda / Q000502:physiology*; D020161:Physical Chromosome Mapping / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D018517:Plant Roots / Q000469:parasitology*; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D005819:Genetic Markers