[Studies on the polysaccharide SGPS2 from the fruits of Siraitia grosvenorii].
OBJECTIVE
To study the structure of the polysaccharides constituents in the fruits of Siraitia grosvenorii (Swingle) C. Jeffrey.


METHODS
SGPS2 was purified by DEAE-cellulose and Sephadex G-200 column chromatography, the component and structure of SGPS2 were analyzed on the basis of spectral and chemical studies by HPLC, IR analysis, partial hydrolysis with acid, methylation analysis, GC and 13C-NMR.


RESULTS
The molecular weight of SGPS2 was 650 000. Polysaccharide was composed of L- made up of (1 --> 2, 4) linked rhamnose, (1 --> 4) linked rhamnose residues in main chain; and (1 --> 2) linked rhamnose, (1 --> 3) linked rhamnose in side chains. Terminal residues attached main chain to rhamnose.


CONCLUSIONS
SGPS2 was composed of rhamnose and curonic acid.
Huang
Cui-Ping
CP
Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, School of Environment and Resource of Guangxi Normal University, Guilin 541004, China.
Li
Jun
J
Liu
Qing-Ye
QY
Lu
Xue-Wen
XW
Meng
Ai-Ping
AP
Zhu
Sheng-Jun
SJ
D002851:Chromatography, High Pressure Liquid; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005965:Glucuronates / Q000032:analysis*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012210:Rhamnose / Q000032:analysis*
D005965:Glucuronates; D011134:Polysaccharides; D012210:Rhamnose