title: Non-FokI-based zinc finger nucleases.
abstract: The design of functional proteins is one of the most challenging areas of protein research. We have constructed zinc finger peptides with metal-dependent hydrolytic abilities by mutating the zinc ligands in classical zinc fingers, without the need to add a FokI or other DNA cleavage domain. The designed peptides acquired DNA cleavage ability successfully, retaining the proper zinc finger folding and DNA targeting ability. We have also succeeded in site-specific DNA cleavage in the presence of cerium ions by introducing a lanthanide ion-binding loop as a linker of zinc finger motifs.
authors.affiliation: Institute for Chemical Research, Kyoto University, Kyoto, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Imanishi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Negi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Sugiura
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002563:Cerium / Q000378:metabolism; D053837:DNA Cleavage; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D010455:Peptides; D002563:Cerium; D004720:Endonucleases