title: Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis.
abstract: OBJECTIVE
  To analyze the effects of hyperbaric oxygen therapy (HBO) on the mechanical resistance of anastomosis performed in rats' distal colon presenting peritonitis induced by ligature and cecal punction using the Total Energy Rupture biomechanical test (ETR).
  
  
  METHODS
  It was used 45 rats divided into three groups of 15 animals each. In Control Group (CG), it was performed anastomosis in distal colon without peritonitis. In Peritonitis Group (PG), it was performed anastomosis six hours after the induction of peritonitis by ligature and cecal punction. In Hyperbaric Chamber Group (HCG), it was performed six hours after the induction of peritonitis by ligature and cecal punction. The animals on CG and PG were kept at place air while the animals on HCG were placed on an experimental hyperbaric chamber in order to inhale oxygen at 100%, two absolute atmospheres, for 120 minutes, for four consecutive days. Euthanasia took place on the fifth day of the experiment. All the animals underwent to Total Energy Rupture Biomechanical Resistance test (ETR). Total Energy Rupture was defined as the necessary internal energy stored up to promote the colon rupture after an external traction force imposition.
  
  
  RESULTS
  The Peritonitis Group presents smaller average ETR than Control Group. There was no statistical difference between Peritonitis Group and Hyberbaric Chamber Group.
  
  
  CONCLUSIONS
  Hyperbaric oxygen therapy did not alter the mechanical resistance of anastomosis performed in distal colon of rats under the presence of peritonitis induced by ligature and cecal puncture.
authors.affiliation: Post-graduate Program in Surgery, University of Campinas (UNICAMP), Campinas, SP, Brazil. angelorocha@terra.com.br
authors.forename: Antonio Angelo
authors.initials: AA
authors.lastname: Rocha
authors.forename: Raquel Franco
authors.initials: RF
authors.lastname: Leal
authors.forename: Maria de Lourdes Setsuko
authors.initials: Mde L
authors.lastname: Ayrizono
authors.forename: Wu Feng
authors.initials: WF
authors.lastname: Chung
authors.forename: Cláudio Saddy Rodrigues
authors.initials: CS
authors.lastname: Coy
authors.forename: Huei Diana
authors.initials: HD
authors.lastname: Lee
authors.forename: João José
authors.initials: JJ
authors.lastname: Fagundes
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002432:Cecum / Q000601:surgery*; D002921:Cicatrix / Q000503:physiopathology; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D010538:Peritonitis / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*