Mapping the interactions between a major pollen allergen and human IgE antibodies.
The interaction of specific IgE antibodies with allergens is a key event in the induction of allergic symptoms, thus representing an important target for therapeutic interventions in Type I allergies. We report here the solution NMR structure of Art v 1, the major mugwort pollen allergen. Art v 1 is the first protein structure with an allergenic defensin fold linked to a polyproline domain, which has not been identified in any reported allergen structure in the PDB. Moreover, the direct interaction of polyclonal IgE antibodies from an allergic patient has been mapped on the surface of an allergen for the first time. The data presented herein provide the basis for the design of tools for safe and effective vaccination against mugwort pollen allergy.
Razzera
Guilherme
G
Centro Nacional de Ressonância Magnética Nuclear, Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica, Rio de Janeiro, Brazil.
Gadermaier
Gabriele
G
de Paula
Viviane
V
Almeida
Marcius S
MS
Egger
Matthias
M
Jahn-Schmid
Beatrice
B
Almeida
Fabio C L
FC
Ferreira
Fatima
F
Valente
Ana Paula
AP
D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011058:Pollen / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013045:Species Specificity; D014611:Vaccination / Q000379:methods
D052179:Antigens, Plant; C102724:Art v 1 protein, mugwort; D010455:Peptides; D010940:Plant Proteins; C011083:polyproline; D007073:Immunoglobulin E