Investigation of plasma-functionalized multiwalled carbon nanotube film and its application of DNA sensor for Legionella pneumophila detection.
A novel multiwall carbon nanotube (MWCNT) electrode functionalized with oxygen plasma treatment was prepared and characterized, and its DNA sensing ability for Legionella pneumophila (L. pneumophila) detection was examined using electrochemical measurement. A well-patterned MWCNT working electrode (WE) on a Pt track was fabricated using photolithography, transfer methods and an etching technique. The MWCNT WE was functionalized by oxygen plasma treatment prior to applying for DNA sensor. The surface morphology of the plasma-functionalized MWCNT (pf-MWCNT) WEs were observed by scanning electron microscope (SEM) and the change of chemical composition was characterized by X-ray photoelectron spectroscopy (XPS), and electrochemical measurements were performed using CV with ferricyanide/ferrocyanide redox couple. Effective areas of working electrodes were calculated to be 0.00453 cm(2) for pristine MWCNT electrode and 0.00747-0.00874 cm(2) for pf-MWCNT electrodes with different plasma treatment times. Differential pulse voltammetry (DPV) was carried out in methylene blue solution for DNA sensing. The pf-MWCNT based DNA sensor was successfully operated in a target concentration range of 10 pM to 100 nM and had a lower detection limit than a pristine MWCNT based DNA sensor.
Park
Eun Jin
EJ
Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Republic of Korea. parkeunjin@korea.ac.kr
Lee
Jun-Yong
JY
Kim
Jun Hyup
JH
Lee
Cheol Jin
CJ
Kim
H Stanley
HS
Min
Nam Ki
NK
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D055664:Electrochemical Techniques / Q000592:standards; D004566:Electrodes / Q000592:standards; D016952:Legionella pneumophila / Q000302:isolation & purification*; D057230:Limit of Detection; D008751:Methylene Blue; D037742:Nanotubes, Carbon / Q000737:chemistry*
D037742:Nanotubes, Carbon; D008751:Methylene Blue