An integrated approach to the degradation of phytates in the corn wet milling process.
An integrated process was developed to hydrolyze the phytates in light steep water (LSW) and to simultaneously isolate inorganic phosphate (Pi) and myo-inositol products. The proposed integrated process will be helpful in resolving the environmental and nutritional concerns in the use of corn gluten feed (CGF) in the animal diets. This process comprised of partial and total hydrolysis of LSW and intermediate anion exchange separation technique. The phytates in LSW were initially degraded to negatively charged myo-inositol phosphates (InsP(2)-InsP(5)). The optimized experimental parameters for the partial hydrolysis of LSW were determined to be 2 h hydrolysis with 1FTU Aspergillus niger/g substrate at 35 degrees C. The negatively charged species of the partially hydrolyzed substrate were separated on a strong base anion exchange resin. The negatively charged species, retained by the resin, were eluded with 1M NaCl solution and were subjected to complete hydrolysis with the Escherichia coli, A. niger derived phytases and their respective combinations. The maximum amount of myo-inositol released from the anion exchange column was 3.73+/-0.03 mg/NaCl elution which was detected after 48 h reactions catalyzed by 100 FTU E. coli, 150 FTU E. coli, and 150 FTU the combination of A. niger and E. coli. The time course of Pi released showed a similar trend to that of myo-inositol and the released Pi reached a maximum amount of 3.30+/-0.05 mg/g NaCl elution after 48 h incubation at the enzyme loadings for which the maximum concentration of myo-inositol were reached.
Noureddini
H
H
Department of Chemical and Biomolecular Engineering, 207H Othmer Hall, University of Nebraska-Lincoln, Lincoln, NE 68588-0643, USA. hnoureddini@unl.edu
Dang
J
J
D010832:6-Phytase / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology; D001709:Biotechnology / Q000379:methods*; D002138:Calibration; D003116:Color; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D007202:Indicators and Reagents; D007294:Inositol / Q000032:analysis; D007295:Inositol Phosphates / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000378:metabolism*; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
D007202:Indicators and Reagents; D007295:Inositol Phosphates; D010710:Phosphates; D014866:Waste Products; D010758:Phosphorus; D007294:Inositol; D010833:Phytic Acid; D010832:6-Phytase