title: A highly selective low-background fluorescent imaging agent for nitric oxide.
abstract: We introduce a novel sensing mechanism for nitric oxide (NO) detection with a particular easily synthesized embodiment (NO(550)), which displays a rapid and linear response to NO with a red-shifted 1500-fold turn-on signal from a dark background. Excellent selectivity was observed against other reactive oxygen/nitrogen species, pH, and various substances that interfere with existing probes. NO(550) crosses cell membranes but not nuclear membranes and is suitable for both intra- and extracellular NO quantifications. Good cytocompatibility was found during in vitro studies with two different cell lines. The high specificity, dark background, facile synthesis, and low pH dependence make NO(550) a superior probe for NO detection when used as an imaging agent.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Yang
authors.forename: Stephanie K
authors.initials: SK
authors.lastname: Seidlits
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Adams
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Lynch
authors.forename: Christine E
authors.initials: CE
authors.lastname: Schmidt
authors.forename: Eric V
authors.initials: EV
authors.lastname: Anslyn
authors.forename: Jason B
authors.initials: JB
authors.lastname: Shear
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009569:Nitric Oxide / Q000737:chemistry*; D012995:Solubility
chemical_list: D005456:Fluorescent Dyes; D009569:Nitric Oxide