Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization.
Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.
Zhang
Xian
X
Institute of Plant Science and Resources, Okayama University 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan.
Tani
Akio
A
Kawai
Fusako
F
Kimbara
Kazuhide
K
D018407:Bacterial Physiological Phenomena; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D016958:Pseudomonas putida / Q000502:physiology