title: Reduction in generation of reactive oxygen species and endothelial dysfunction during postprandial state.
abstract: OBJECTIVE
  To characterise changes in generation of cellular reactive oxygen species (ROS) in healthy males during the postprandial state, and to analyse the influence of the postprandial state on endothelial ROS generation and endothelial dysfunction.
  
  
  RESULTS
  Seventeen healthy subjects were recruited. Blood samples were collected in the fasting state and 2, 4, 6 and 8h after liquid-meal intake (composition: 25% fat, 55% dextromaltose and 14% protein), providing 40 gfat m(-2) body surface. Plasma lipids, apolipoproteins, glucose and insulin were measured during this period. Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation. The influence of postprandial state on intracellular ROS generation was measured by two different methods in PBMCs and in a human immortalised endothelial cell line (ECV 304). Artery flow-mediated vasodilation (FMD) was used to evaluate the endothelial function, and oxygen consumption by PBMCs was measured. Reduced ROS generation was observed in all methods and cells during the postprandial period. FMD was impaired 8h after meal intake (23±6 vs. 13±2, P<0.05 vs. baseline). The consumption of oxygen was reduced in PBMCs (-14% into 2h, P<0.05 vs. baseline and -27% after 4h, P<0.01 vs. baseline). ROS generation was correlated with plasma lipids, insulin, apolipoproteins and oxygen consumption.
  
  
  CONCLUSIONS
  In contrast to the previously reported elevation of postprandial oxidative stress, this study shows reduced ROS generation in PBMCs and in ECV 304. Data obtained in both cellular models suggest the existence of a protective response against plasma postprandial oxidative stress.
authors.affiliation: Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, Campinas, São Paulo, Brazil. fabiosodre@hotmail.com
authors.forename: F L
authors.initials: FL
authors.lastname: Sodré
authors.forename: B A
authors.initials: BA
authors.lastname: Paim
authors.forename: A
authors.initials: A
authors.lastname: Urban
authors.forename: A E
authors.initials: AE
authors.lastname: Vercesi
authors.forename: E C
authors.initials: EC
authors.lastname: Faria
mesh_terms: D000328:Adult; D001053:Apolipoproteins / Q000097:blood; D002461:Cell Line, Transformed; D004730:Endothelium, Vascular / Q000502:physiology*; D005502:Food; D006801:Humans; D007328:Insulin / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D010101:Oxygen Consumption; D019518:Postprandial Period; D017382:Reactive Oxygen Species / Q000097:blood*; D014471:Umbilical Veins; D014664:Vasodilation / Q000502:physiology
chemical_list: D001053:Apolipoproteins; D007328:Insulin; D008055:Lipids; D017382:Reactive Oxygen Species