Molecular breeding of tomato lines for mass production of miraculin in a plant factory.
A transgenic tomato line (56B, "Moneymaker") that expresses the miraculin gene driven by the CaMV 35S promoter was crossed with a dwarf tomato ("Micro-Tom") for the molecular breeding of cultivars that are suitable for miraculin production in a closed cultivation system. Plant size, miraculin accumulation, and self-pruning growth were used as selection indicators for F2 plants. Two lines were chosen for further analysis, bred to the F6 or F7 generation and cultivated in a closed cultivation system. In 56B and the two crossed lines, the concentrations of miraculin in the pericarp were 140, 367, and 343 microg/g FW, respectively. We also estimated that 26.2, 73.6, and 45.9 kg FW/m2 of tomatoes and 2.2, 16.6, and 9.8 mg/m2 of miraculin in the pericarp, respectively, could be harvested per year. These two crossed lines will be useful for the mass production of miraculin, especially in a closed cultivation system.
Kato
Kazuhisa
K
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
Yoshida
Riichiro
R
Kikuzaki
Ayako
A
Hirai
Tadayoshi
T
Kuroda
Hirofumi
H
Hiwasa-Tanase
Kyoko
K
Takane
Kenichi
K
Ezura
Hiroshi
H
Mizoguchi
Tsuyoshi
T
D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D030821:Plants, Genetically Modified
D006023:Glycoproteins