title: Detection of waterborne parasites using field-portable and cost-effective lensfree microscopy.
abstract: Protection of human health and well-being through water quality management is an important goal for both the developed and the developing parts of the world. In the meantime, insufficient disinfection techniques still fail to eliminate pathogenic contaminants in freshwater as well as recreational water resources. Therefore, there is a significant need for screening of water quality to prevent waterborne outbreaks and incidents of water-related diseases. Toward this end, here we investigate the use of a field-portable and cost-effective lensfree holographic microscope to image and detect pathogenic protozoan parasites such as Giardia Lamblia and Cryptosporidium Parvum at low concentration levels. This compact lensless microscope (O. Mudanyali et al., Lab Chip, 2010, 10, 1417-1428), weighing approximately 46 grams, achieves a numerical aperture of approximately 0.1-0.2 over an imaging field of view that is more than an order of magnitude larger than a typical 10X objective lens, and therefore may provide an important high-throughput analysis tool for combating waterborne diseases especially in resource limited settings.
authors.affiliation: Electrical Engineering Department, UCLA, CA 90095, USA.
authors.forename: Onur
authors.initials: O
authors.lastname: Mudanyali
authors.forename: Cetin
authors.initials: C
authors.lastname: Oztoprak
authors.forename: Derek
authors.initials: D
authors.lastname: Tseng
authors.forename: Anthony
authors.initials: A
authors.lastname: Erlinger
authors.forename: Aydogan
authors.initials: A
authors.lastname: Ozcan
mesh_terms: D000818:Animals; D003362:Cost-Benefit Analysis; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D016829:Giardia lamblia / Q000302:isolation & purification*; D006696:Holography; D008853:Microscopy / Q000191:economics* / Q000379:methods*; D014867:Water / Q000469:parasitology*
chemical_list: D014867:Water