title: Estrogen sulfotransferase regulates body fat and glucose homeostasis in female mice.
abstract: Estrogen regulates fat mass and distribution and glucose metabolism. We have previously found that estrogen sulfotransferase (EST), which inactivates estrogen through sulfoconjugation, was highly expressed in adipose tissue of male mice and induced by testosterone in female mice. To determine whether inhibition of estrogen in female adipose tissue affects adipose mass and metabolism, we generated transgenic mice expressing EST via the aP2 promoter. As expected, EST expression was increased in adipose tissue as well as macrophages. Parametrial and subcutaneous inguinal adipose mass and adipocyte size were significantly reduced in EST transgenic mice, but there was no change in retroperitoneal or brown adipose tissue. EST overexpression decreased the differentiation of primary adipocytes, and this was associated with reductions in the expression of peroxisome proliferator-activated receptor-γ, fatty acid synthase, hormone-sensitive lipase, lipoprotein lipase, and leptin. Serum leptin levels were significantly lower in EST transgenic mice, whereas total and high-molecular-weight adiponectin levels were not different in transgenic and wild-type mice. Glucose uptake was blunted in parametrial adipose tissue during hyperinsulinemic-euglycemic clamp in EST transgenic mice. In contrast, hepatic insulin sensitivity was improved but muscle insulin sensitivity did not change in EST transgenic mice. These results reveal novel effects of EST on adipose tissue and glucose homeostasis in female mice.
authors.affiliation: Department of Medicine and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Victor K
authors.initials: VK
authors.lastname: Khor
authors.forename: Ravindra
authors.initials: R
authors.lastname: Dhir
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yin
authors.forename: Rexford S
authors.initials: RS
authors.lastname: Ahima
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Song
mesh_terms: D052438:Adipocytes, White / Q000201:enzymology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D001823:Body Composition / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D004967:Estrogens / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000097:blood; D005260:Female; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D007333:Insulin Resistance / Q000502:physiology*; D020738:Leptin / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D047495:PPAR gamma / Q000097:blood; D011897:Random Allocation; D002787:Sterol Esterase / Q000097:blood; D015238:Sulfotransferases / Q000378:metabolism*
chemical_list: D004967:Estrogens; D020738:Leptin; D047495:PPAR gamma; D064429:Fatty Acid Synthases; D015238:Sulfotransferases; C020122:estrone sulfotransferase; D002787:Sterol Esterase; D008071:Lipoprotein Lipase; D005947:Glucose