Application of mixed mode resins for the purification of antibodies.
The downstream processing of monoclonal antibodies from cell culture supernatant is usually done by a number of chromatographic and non-chromatographic steps. Efforts are taken to reduce the costs associated to those steps, while maintaining a high product purity. A possibility to reach this goal is the reduction of the number of chromatographic steps using mixed mode resins that offer more than one functionality in one chromatographic step. In this work, a commercially available mixed mode resin was evaluated systematically with respect to the adsorption of proteins. The Henry coefficient, which quantifies the adsorption strength, was measured for the full working range of the stationary phase as a function of the salt concentration and the pH. The results were compared to a conventional anion exchange and a hydrophobic interaction resin. Furthermore, the resin was applied for the polishing step of an antibody from an industrial clarified cell culture supernatant.
Voitl
Agnes
A
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.
Müller-Späth
Thomas
T
Morbidelli
Massimo
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D017077:Culture Media, Conditioned; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D016062:Porosity; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D011506:Proteins; D012965:Sodium Chloride