title: [Study on the longevity related mitochondrial genome variation in Bama elderly population in Guangxi province].
abstract: OBJECTIVE
  To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi.
  
  
  METHODS
  Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism (SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi.
  
  
  RESULTS
  The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls. And it was significantly higher in controls than that in long-lived population (P<0.05).
  
  
  CONCLUSIONS
  The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.
authors.affiliation: Guangxi Jiangbin Hospital, Nanning, Guangxi, 530021 PR China. lvzeping@163.com
authors.forename: Ze-ping
authors.initials: ZP
authors.lastname: Lv
authors.forename: Chen-guang
authors.initials: CG
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Kong
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Jiang
authors.forename: Cai-you
authors.initials: CY
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lv
authors.forename: Gen-fa
authors.initials: GF
authors.lastname: Zhang
authors.forename: Ze
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000368:Aged; D002681:China / Q000208:ethnology; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008136:Longevity / Q000235:genetics*; D009154:Mutation; D002052:Myanmar / Q000208:ethnology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044382:Population Groups
chemical_list: D004272:DNA, Mitochondrial