title: The synergy of 6-O-sulfation and N- or 3-O-sulfation of chitosan is required for efficient inhibition of P-selectin-mediated human melanoma A375 cell adhesion.
abstract: We prepared chitosan sulfated derivatives to address the common structural requirement of the sulfate pattern to block P-selectin-mediated tumor cell adhesion. Our results indicate that 6-O-sulfation of chitosan is indispensable for inhibition of P-selectin binding to human melanoma A375 cells. Furthermore, additional N-sulfation or 3-O-sulfation dramatically enhanced the inhibitory activity of 6-O-sulfated chitosan, suggesting that efficient anti-P-selectin adhesion activity of sulfated saccharides requires the synergy of 6-O-sulation and N- or 3-O-sulfation in glucosamine units.
authors.affiliation: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Renmin Street, Chanchun, PR China.
authors.forename: Ruifei
authors.initials: R
authors.lastname: Wang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008545:Melanoma / Q000473:pathology*; D010100:Oxygen / Q000737:chemistry*; D019007:P-Selectin / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D019007:P-Selectin; D013431:Sulfates; D048271:Chitosan; D010100:Oxygen