Reduced vasoreactivity in corpus cavernosum of the akita mouse.
Diabetes Mellitus (DM) is an important risk factor of erectile dysfunction (ED). We sought to investigate the nature and mechanism of the vasoreactivity in Akita (Ins2 [WT/C96Y]) mice, a model of genetic nonobese Type 1 diabetes that recapitulates human Type 1 diabetes. Eight wild-type (WT) mice (group 1) and 8 Akita mice (group 2) were used for this study. Corporal tissues were harvested and studied for endothelium-dependent and endothelium-independent vasoreactivities by isometric tension study; levels of vascular endothelial growth factor (VEGF) and cyclic guanosine monophosphate (cGMP) were studied with the use of enzyme-linked immunosorbent assay (ELISA). Endothelium-dependent and endothelium-independent vasoreactivities, cGMP, and VEGF were significantly decreased in the corporal tissues of Akita mice. Corporal tissues from Akita mice demonstrate many of the major functional and biochemical changes found in humans with ED. This model could serve as a valuable tool for advancing our understanding of the role DM plays in the pathogenesis of ED.
Jia
Lijing
L
Department of Joint Surgery, The 3rd Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, Hebei, China.
Xie
Donghua
D
Wang
Xiuli
X
Liu
Yan
Y
Li
Yukun
Y
Zhang
Xiaoyang
X
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000209:etiology* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology; D019001:Nitric Oxide Synthase / Q000032:analysis; D009599:Nitroprusside / Q000494:pharmacology; D010413:Penis / Q000098:blood supply* / Q000503:physiopathology*; D042442:Vascular Endothelial Growth Factors / Q000032:analysis / Q000502:physiology; D014664:Vasodilation
D001786:Blood Glucose; D042442:Vascular Endothelial Growth Factors; D009599:Nitroprusside; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D000109:Acetylcholine