Conjugated polymer nanoparticles for drug delivery and imaging.
We prepared a new conjugated polymer nanoparticle with the size of about 50 nm that is prepared by electrostatic assembly of cationic conjugated polymer PFO and anionic poly(l-glutamic acid) conjugated with anticancer drug doxorubicin (PFO/PG-Dox). The PFO exhibits good fluorescence quantum yield, photostability, and little cytotoxicity to meet the essential requests for cell imaging. In PFO/PG-Dox nanoparticles, the fluorescence of PFO is highly quenched by Dox by electron transfer mechanism, and thus the PFO is in the fluorescence "turn-off' state. After PFO/PG-Dox nanoparticles are exposed to carboxypeptidase or are taken up by cancer cells, the poly(l-glutamic acid) is hydrolysed to release the Dox, inducing the activation of PFO fluorescence to "turn-on" state. This multifunctional nanoparticle system can deliver Dox to targeted cancer cells and monitor the Dox release based on fluorescence "turn-on" signal of PFO, which concurrently images the cancer cells. The present work opens the door for new functional studies of conjugated polymer in simultaneous imaging and disease therapeutics.
Feng
Xuli
X
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Lv
Fengting
F
Liu
Libing
L
Tang
Hongwei
H
Xing
Chengfen
C
Yang
Qiong
Q
Wang
Shu
S
D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems / Q000379:methods*; D005449:Fluorenes / Q000493:pharmacokinetics; D005453:Fluorescence; D018698:Glutamic Acid / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D053758:Nanoparticles; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D005449:Fluorenes; D011108:Polymers; C542782:poly(9,9-di-n-octyl-2,7-fluorene); D018698:Glutamic Acid; D004317:Doxorubicin