Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced cytogenetic damage in rat bone-marrow.
The cytogenetic damage inflicted by the synthetic pyrethroid insecticide cypermethrin (CYP) on the bone-marrow of male white rats, as well as possible protective role of two natural elements: garlic extract (GRE, 500mg/kg) and vitamin C (VTC, 20mg/kg) against the mutagenic potential of the insecticide were assessed. CYP was orally intubated in a single treatment (1/2 LD(50)) or in repeated treatments (1/5 LD(50) daily, for 5 successive days), either alone, or concomitantly with repeated oral intubations (5 successive days) of each individual putative protector, or with their combination (GRE or/and VTC). One hundred and twenty male rats were divided over into five groups of each 24 animals. The groups received nothing, a single dose or repeated treatments with insecticide alone, or associated with putative natural elements, separately or in combinations. Animals were sacrificed at their scheduled times and their femoral bone-marrows were flushed out to be utilized in the micronucleus test and metaphase chromosomal aberration assay. The results show that CYP administration significantly induced clastogenic effects, as revealed by the significant increase in the mean frequencies of micronucleated polychromatic erythrocytes and various structural chromosomal aberrations in bone-marrow metaphase cells of all groups of treated rats. On the other hand, this investigation clearly revealed the protective role of GRE and VTC, either each alone or in combination, against the mutagenic potential of cypermethrin: the garlic extract was often more efficient in its protective action against the insecticide toxicity than vitamin C. while the combination of both natural elements produced, in most cases, a more pronounced protective effect than when each was administered alone.
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Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Menoufiya University, Sadat City Branch, Sadat City, Egypt. memzat68@yahoo.com
Khalaf
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Salem
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D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001853:Bone Marrow / Q000648:ultrastructure; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D005737:Garlic; D007306:Insecticides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D010936:Plant Extracts / Q000494:pharmacology*; D011722:Pyrethrins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D007306:Insecticides; D010936:Plant Extracts; D011722:Pyrethrins; C017160:cypermethrin; D001205:Ascorbic Acid