title: Screening for methylation-silenced genes in acute myeloid leukemia HL-60 cell line by a quantitative proteomic approach.
abstract: OBJECTIVE
  To screen for new methylation association genes in HL-60 to reveal the pathogenesis of leukemia, and provide important theoretical and scientific basis for the prevention and cure of leukemia.
  
  
  METHODS
  Two-dimensional fluorescence difference gel electrophoresis (F-2D-DIGE) was performed to separate the total proteins from acute myelogenous leukemia (AML) cell line HL-60 cells with or without 5-aza-2-deoxycytidine (5-aza-2-dC) treatment. Imaging software Decyder 6.5 and PDQuest were used to detect the differential expression protein spots, and matrix-assisted laser desorption/ionizaion time-of-flight mas spectrometer (MALDI-TOF MS) was adopted to identify the differential expression proteins.
  
  
  RESULTS
  F-2D-DIGE maps of 5-aza-2-dC-untreated HL-60 and-treated HL-60 cells were established. A total of 53 differential protein spots were detected, and 35 differential proteins were successfully identified. Of the identified proteins, 32 proteins were up-regulated, and 3 proteins were down-regulated in HL-60 cells after 5-aza-2-dC treatment.
  
  
  CONCLUSIONS
  Thirty-five differential proteins may be associated with methylation in HL-60 cell line, which provides the important clues for epigenetic study of leukemia.
authors.affiliation: Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Can'e
authors.initials: C
authors.lastname: Tang
authors.forename: Tan
authors.initials: T
authors.lastname: Tan
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Ruan
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
authors.forename: Maoyu
authors.initials: M
authors.lastname: Li
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D000077209:Decitabine; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D020868:Gene Silencing; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D000077209:Decitabine; D015254:DNA Modification Methylases; D001374:Azacitidine