[Chinese medicinal monomer and compound for 60Co-gamma-induced spermatogenic disturbance in mice].
OBJECTIVE
To explore the effects of the monomer and compound of the Chinese herbal drugs resveratrol, lycium barbarum polysaccharide (LBP) and icariin on 60Co-gamma-induced spermatogenic disturbance in mice based on the theory of modern Chinese medicine.


METHODS
A total of 105 male Kunming mice were randomly divided into seven groups, with 15 in each. Group A were normally raised and Groups B, C, D, E, F and G irradiated by 60Co-gamma 6 Gy followed by 60Co-gamma 4 Gy at the interval of 7 days. A week later, Groups C, D, E, F and G received intragastrically the suspension of resveratrol, resveratrol + LBP, resveratrol + icariin, resveratrol + LBP + icariin and resveratrol + LBP + icariin + L-carnitine, respectively, at the dose of 80 mg/(kg x d) for 60 days. The general condition, physical signs and body weight changes of the mice were recorded, and 24 hours after the intragastric medication, their testes were harvested to obtain the testicular weight and indexes, the levels of FSH, LH, T and E2 determined by ELISA, the T/E2 ratio calculated, and the histology of the testis tissues observed under the microscope.


RESULTS
The testicular indexes of the mice were decreased by radiation-induced damage, but restored to some extent after intragastric medication, especially in Groups E, F and G. The levels of FSH, LH and T were obviously improved by LBP. The T level and testis weight were increased by Icariin. The level of T/E2 was elevated in Groups E, F and G. The best results were achieved in Group F, which exhibited almost complete recovery from reproductive endocrine disorder and spermatogenic damage.


CONCLUSIONS
The Chinese medicinal monomer is effective for 60Co-gamma-induced spermatogenic disturbance in mice, and the compound suspension of resveratrol + LBP + icariin produces the best result.
Zhang
Wei-xing
WX
Department of Urology, The First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Clinical Medical Disciplines for Henan Higher Education Institutions, Zhengzhou University Institute of Cancer Molecular Surgery, Zhengzhou, Henan 450052, China.
Wang
Hua-li
HL
Wang
Rui
R
Li
Rui
R
He
Wei
W
Zhang
Tian-biao
TB
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005720:Gamma Rays / Q000009:adverse effects; D007248:Infertility, Male / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D013091:Spermatogenesis / Q000528:radiation effects*; D013737:Testis / Q000473:pathology / Q000528:radiation effects
D004365:Drugs, Chinese Herbal