Protein matrices for improved wound healing: elastase inhibition by a synthetic peptide model.
The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman-Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase-antielastase imbalance found on chronic wounds.
Vasconcelos
Andreia
A
Universidade do Minho, Departamento de Engenharia Têxtil, Campus de Azurém, 4800-058 Guimarães, Portugal. andreiav@det.uminho.pt
Pêgo
Ana Paula
AP
Henriques
Lara
L
Lamghari
Meriem
M
Cavaco-Paulo
Artur
A
D000818:Animals; D012831:Bombyx; D049109:Cell Proliferation / Q000187:drug effects; D005349:Fibroins / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D010446:Peptide Fragments; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors; C482288:fibroin, silkworm; D007633:Keratins; D005349:Fibroins; D010196:Pancreatic Elastase