title: A simple method of surface functionalisation for immuno-specific immobilisation of proteins.
abstract: We present a new and advanced methodology, developed for surface functionalisation of gold and to study immobilisation of an immuno-specific system of proteins. A combination of electrochemical quartz crystal microbalance and Raman spectroscopy techniques allowed a complete understanding of the system starting from surface functionalisation and progressing to the functional structure analysis of immobilised proteins. A simple electrochemical procedure was formulated to prepare sulphonyl chloride terminated gold surfaces that form a strong sulphonamide bond with the receptor protein staphylococcal protein A (SpA). On the SpA grafted surfaces, the immobilisation of a human IgG and consecutive binding of an immuno-specific anti-human IgG was observed. The surface functional groups form a strong interaction with SpA without disturbing its functional properties. The native functional structure of SpA and also the IgGs was found to be retained in their immobilised state.
authors.affiliation: Laboratoire PEC, UMR CNRS 6087, Université du Maine, A.O. Messiaen, 72085, Le Mans, France.
authors.forename: R P
authors.initials: RP
authors.lastname: Kengne-Momo
authors.forename: Y L
authors.initials: YL
authors.lastname: Jeyachandran
authors.forename: A
authors.initials: A
authors.lastname: Assaf
authors.forename: C
authors.initials: C
authors.lastname: Esnault
authors.forename: P
authors.initials: P
authors.lastname: Daniel
authors.forename: J F
authors.initials: JF
authors.lastname: Pilard
authors.forename: M J
authors.initials: MJ
authors.lastname: Durand
authors.forename: F
authors.initials: F
authors.lastname: Lagarde
authors.forename: E
authors.initials: E
authors.lastname: Dongo
authors.forename: G
authors.initials: G
authors.lastname: Thouand
mesh_terms: D004563:Electrochemistry; D007074:Immunoglobulin G / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013205:Staphylococcal Protein A / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D013205:Staphylococcal Protein A