title: Steamed ginseng-leaf components enhance cytotoxic effects on human leukemia HL-60 cells.
abstract: Three new dammarane-type glycosides, named ginsenosides SL(1)-SL(3) (1-3), and eleven known compounds (4-14) were isolated from the heat-processed leaves of Panax ginseng. Their structures were elucidated on the basis of extensive chemical and spectroscopic methods. Cytotoxic-activity testing of compounds 1-14 against human leukemia HL-60 cells showed that ginsenosides Rh(3) (11) and Rk(2) (12) exhibited potent effects with IC(50) values of 0.8 and 0.9 microM. In addition, ginsenosides SL(3) (3), 20S-Rg(2) (7), F(4) (10), 20S-Rh(2) (13) displayed strong activity with IC(50) values of 9.0, 9.0, 7.5, and 8.2 microM, respectively. This is the first report on chemical components of the steamed ginseng leaves.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Nguyen Huu
authors.initials: NH
authors.lastname: Tung
authors.forename: Gyu Yong
authors.initials: GY
authors.lastname: Song
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Long Guo
authors.initials: LG
authors.lastname: Jin
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Boo
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008968:Molecular Conformation; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D036145:Ginsenosides; D010936:Plant Extracts; C554027:ginsenoside SL1; C554030:ginsenoside SL2; C554029:ginsenoside SL3