The novel responses of ethambutol against Mycobacterium smegmatis mc²155 Revealed by proteomics analysis.
Ethambutol (EMB), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study. We compared the protein profiles on M. smegmatis mc²155 treated by EMB and untreated using fluorescence difference two-dimensional gel electrophoresis (2-D DIGE). A total of 40 differential protein spots were selected and 22 proteins were identified by HPLC-nano ESI-MS/MS analysis, including 16 over-expressed proteins and 6 under-expressed proteins. These proteins mainly affected energy metabolism, as well as synthesis and modification of macromolecules. The expressions of correspondent genes were confirmed by RT-PCR. This investigation provided some clues for searching potential drug targets.
Jiang
Tao
T
Department of Biotechnology, Dalian Medical University, Dalian, 116044, China.
Zhan
Yaoyao
Y
Sun
Mingzhong
M
Liu
Shuqing
S
Zang
Shizhu
S
Ma
Yufang
Y
Xin
Yi
Y
D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004977:Ethambutol / Q000494:pharmacology*; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D020543:Proteome / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D000995:Antitubercular Agents; D001426:Bacterial Proteins; D020543:Proteome; D004977:Ethambutol