title: Identification and characterization of toxigenic fusaria associated with sorghum grain mold complex in India.
abstract: Fusarium species are dominant within the sorghum grain mold complex. Some species of Fusarium involved in grain mold complex produce mycotoxins, such as fumonisins. An attempt was made to identify Fusarium spp. associated with grain mold complex in major sorghum-growing areas in India through AFLP-based grouping of the isolates and to further confirm the species by sequencing part of α-Elongation factor gene and comparing the sequences with that available in the NCBI database. The dendrogram generated from the AFLP data clustered the isolates into 5 groups. Five species of Fusarium--F. proliferatum, F. thapsinum, F. equiseti, F. andiyazi and F. sacchari were identified based on sequence similarity of α-Elongation factor gene of the test isolates with those in the NCBI database. Fusarium thapsinum was identified as predominant species in Fusarium--grain mold complex in India and F. proliferatum as highly toxigenic for fumonisins production. Analysis of molecular variance (AMOVA) revealed 54% of the variation in the AFLP patterns of 63 isolates was due to the differences between Fusarium species, and 46% was due to differences between the strains within a species.
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad, Andhra Pradesh 502324, India. r.sharma@cgiar.org
authors.forename: Rajan
authors.initials: R
authors.lastname: Sharma
authors.forename: Ram P
authors.initials: RP
authors.lastname: Thakur
authors.forename: Senapathy
authors.initials: S
authors.lastname: Senthilvel
authors.forename: Spurthi
authors.initials: S
authors.lastname: Nayak
authors.forename: S Veera
authors.initials: SV
authors.lastname: Reddy
authors.forename: Veeranki Panduranga
authors.initials: VP
authors.lastname: Rao
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Varshney
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry; D005670:Fusarium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014644:Genetic Variation; D007194:India; D010445:Peptide Elongation Factors / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D037341:Fumonisins; D010445:Peptide Elongation Factors