title: Eicosanoids in the innate immune response: TLR and non-TLR routes.
abstract: The variable array of pattern receptor expression in different cells of the innate immune system explains the induction of distinct patterns of arachidonic acid (AA) metabolism. Peptidoglycan and mannan were strong stimuli in neutrophils, whereas the fungal extract zymosan was the most potent stimulus in monocyte-derived dendritic cells since it induced the production of PGE(2), PGD(2), and several cytokines including a robust IL-10 response. Zymosan activated kappaB-binding activity, but inhibition of NF-kappaB was associated with enhanced IL-10 production. In contrast, treatments acting on CREB (CRE binding protein), including PGE(2), showed a direct correlation between CREB activation and IL-10 production. Therefore, in dendritic cells zymosan induces il10 transcription by a CRE-dependent mechanism that involves autocrine secretion of PGE(2), thus unraveling a functional cooperation between eicosanoid production and cytokine production.
authors.affiliation: Instituto de Biología y Genética Molecular, Valladolid, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Alvarez
authors.forename: Isela
authors.initials: I
authors.lastname: Valera
authors.forename: Cristina
authors.initials: C
authors.lastname: Municio
authors.forename: Etzel
authors.initials: E
authors.lastname: Hugo
authors.forename: Francisco
authors.initials: F
authors.lastname: Padrón
authors.forename: Lydia
authors.initials: L
authors.lastname: Blanco
authors.forename: Mario
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Nieves
authors.initials: N
authors.lastname: Fernández
authors.forename: Mariano Sánchez
authors.initials: MS
authors.lastname: Crespo
mesh_terms: D001483:Base Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D015777:Eicosanoids / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000276:immunology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology*; D015054:Zymosan / Q000276:immunology
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D015777:Eicosanoids; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D016328:NF-kappa B; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D051193:Toll-Like Receptors; D016753:Interleukin-10; D015054:Zymosan; D051546:Cyclooxygenase 2