Practical preparation of epilactose produced with cellobiose 2-epimerase from Ruminococcus albus NE1.
A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield.
Saburi
Wataru
W
Nihon Shokuhin Kako Co., Ltd, Tajima, Fuji, Shizuoka, Japan. wataru.saburi@nisshoku.co.jp
Yamamoto
Takeshi
T
Taguchi
Hidenori
H
Hamada
Shigeki
S
Matsui
Hirokazu
H
D002475:Cellobiose / Q000378:metabolism*; D002845:Chromatography; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D019998:Racemases and Epimerases / Q000378:metabolism*; D045857:Ruminococcus / Q000201:enzymology*
D004187:Disaccharides; D002475:Cellobiose; C047464:epilactose; D019998:Racemases and Epimerases