title: [Effects of yikunning on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period].
abstract: OBJECTIVE
  To investigate the effects of Yikunning (YKN, Chinese Traditional Medicine) on the expressions of bcl-2 and bax in rat ovaries during perimenopausal period.
  
  
  METHODS
  Thirty female Wistar rats during perimenopausal period were selected by unforced aging. Then the rats were divided into 3 groups randomly: YKN group, Livial control group and Aged control group. Ten young female Wistar rats were selected as young control group. Intragastric administrations were conducted for 4 weeks once daily continuously. The expressions of Bcl-2 Bax mRNAs and proteins in rat ovaries were detected by RT-PCR and Western blot.
  
  
  RESULTS
  The levels of Bcl-2, Bax mRNAs and proteins in rat ovaries in YKN group were higher than those in Aged control group, which showed differences among them (P < 0.01). The Bcl-2/Bax mRNA rate and protein rate in rat ovaries in YKN group were higher than those in Aged control group, which showed differences among them (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  YKN could increase the expressions of Bcl-2, Bax mRNAs and proteins and up-regulate the Bcl-2/Bax mRNA rate, protein rate in rat ovaries during perimenopausal period, which might be one of the molecular mechanisms of YKN postponed the ovarian failure and cured perimenopausal syndrome.
authors.affiliation: Department of Physiology, Harbin Medical University, Harbin 150081, China.
authors.forename: Qing-zhi
authors.initials: QZ
authors.lastname: Cui
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Tian
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Xiao
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Wen
authors.forename: Guang-mei
authors.initials: GM
authors.lastname: Zhang
authors.forename: Guo-yi
authors.initials: GY
authors.lastname: Liu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D010053:Ovary / Q000378:metabolism*; D047648:Perimenopause / Q000187:drug effects* / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein