title: Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano.
abstract: We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Horikawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuda
authors.forename: Kentarou
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Toru
authors.initials: T
authors.lastname: Matsu-ura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Michikawa
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Mikoshiba
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D004023:Dictyostelium; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D007202:Indicators and Reagents / Q000032:analysis / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D015398:Signal Transduction; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D002118:Calcium