title: Rhodamines in the gas phase: cations, neutrals, anions, and adducts with metal cations.
abstract: Optical spectroscopy of biological molecules in the gas phase has recently gained considerable attention, being able to provide complementary structural information in the absence of native matrix. Biomolecules can change their properties when brought into the gas phase, and so can chromophores associated with them. Understanding the photophysics of chromophore labels is central for the correct interpretation of experimental data. In this report, the structure and the optical properties of Rhodamine 19 (R19) in the gas phase were examined by a combination of Fourier-transform ion cyclotron resonance mass spectrometry and visible-light laser spectroscopy. While R19 in solution is found either in neutral (R19(n)) or protonated (R19+H(+)) forms, other structures can be generated in the gas phase, such as anions (R19-H(-)) and adducts with metal cations (R19+M(+)). Experimental evidence for the lactone structure of neutral gas-phase R19 is presented for the first time. The different properties of gas-phase compared to solution-phase R19 are discussed in view of structural analysis of labeled gas-phase biological molecules by optical spectroscopy.
authors.affiliation: Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zürich, Switzerland.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Chingin
authors.forename: Roman M
authors.initials: RM
authors.lastname: Balabin
authors.forename: Konstantin
authors.initials: K
authors.lastname: Barylyuk
authors.forename: Huanwen
authors.initials: H
authors.lastname: Chen
authors.forename: Vladimir
authors.initials: V
authors.lastname: Frankevich
authors.forename: Renato
authors.initials: R
authors.lastname: Zenobi
mesh_terms: D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D005583:Fourier Analysis; D005740:Gases / Q000737:chemistry; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D000838:Anions; D002412:Cations; D005740:Gases; D008670:Metals; D012235:Rhodamines