Active targeting behaviors of biotinylated pluronic/poly(lactic acid) nanoparticles in vitro through three-step biotin-avidin interaction.
In order to prepare targeted drug carriers, previously a biotin group has been attached by our group to the end of Pluronic F87/poly(lactic acid) and Pluronic P85/poly(lactic acid) block co-polymers to obtain B-F87-PLA and B-P85-PLA, respectively. In this paper, the active targeting properties of B-F87-PLA and B-P85-PLA nanoparticles in vitro were investigated through a three-step biotin-avidin interaction by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) tests and fluorescence microscopy (FM). Two kinds of human ovarian cancer cells (OVCAR-3 and SKOV-3) and paclitaxel were chosen for the cytotoxicity tests. CA-125 antigen is over-expressed on OVCAR-3 cells but not on SKOV-3 cells. The loading and release behavior of paclitaxel loaded in B-Pluronic-PLA nanoparticles were also studied. Paclitaxel loaded in both B-F87-PLA and B-P85-PLA nanoparticles shows an initial rapid release followed by a slow release period. Compared with SKOV-3 cells, the cytotoxicity results implied that paclitaxel-loaded B-Pluronic-PLA nanoparticles were delivered more effectively to OVCAR-3 cells due to the specific interaction between the biotin groups on the surface of B-Pluronic-PLA nanoparticles and the avidin/biotinylated MAb X306/CA-125 antigen complexes on the surface of OVCAR-3 cells. The active targeting properties of B-F87-PLA nanoparticles were further confirmed by FM.
Xiong
Xiang Yuan
XY
School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, PR China. xyxiong@gmail.com
Gong
Yan Chun
YC
Li
Zi Ling
ZL
Li
Yu Ping
YP
Guo
Liang
L
D001360:Avidin / Q000378:metabolism*; D001692:Biological Transport; D001710:Biotin / Q000737:chemistry* / Q000378:metabolism*; D018394:CA-125 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011060:Poloxalene / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding
D018394:CA-125 Antigen; D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D001360:Avidin; D019344:Lactic Acid; C033616:poly(lactide); D001710:Biotin; D011060:Poloxalene; D017239:Paclitaxel