title: Live-cell super-resolution imaging with trimethoprim conjugates.
abstract: The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.
authors.affiliation: Department of Chemistry, Columbia University, New York, NY, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Wombacher
authors.forename: Meike
authors.initials: M
authors.lastname: Heidbreder
authors.forename: Sebastian
authors.initials: S
authors.lastname: van de Linde
authors.forename: Michael P
authors.initials: MP
authors.lastname: Sheetz
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann
authors.forename: Virginia W
authors.initials: VW
authors.lastname: Cornish
authors.forename: Markus
authors.initials: M
authors.lastname: Sauer
mesh_terms: D002470:Cell Survival; D006367:HeLa Cells; D006657:Histones / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity; D014295:Trimethoprim / Q000737:chemistry*
chemical_list: D006657:Histones; D014295:Trimethoprim