Increased levels of soluble LR11 in cerebrospinal fluid of patients with Alzheimer disease.
BACKGROUND
Recent genetic and pathological studies have suggested that a lipoprotein receptor, LR11, is intricately implicated in the pathogenesis of Alzheimer disease (AD). We have recently established a novel sandwich ELISA, which enabled the sensitive quantification of a soluble LR11 (sLR11). By this ELISA, we attempted to determine the difference in the levels of CSF sLR11 in AD patients.


METHODS
We examined CSF from 29 AD patients, 20 frontotemporal lobar degeneration patients and 27 age-matched control subjects. The CSF sLR11 level as well as the levels of tau and beta-amyloid42 (Abeta42) were determined by sandwich ELISA.


RESULTS
The CSF tau level and tau/Abeta42 ratio were significantly increased (p < 0.01) in the AD patients. The CSF sLR11 level in the AD patients was significantly higher (p < 0.01) than that of the frontotemporal lobar degeneration patients and the controls. The APOE-epsilon4-positive AD patients have higher sLR11 levels than the APOE-epsilon4-negative patients (p < 0.01).


CONCLUSIONS
These results suggest that the quantification of CSF sLR11 may serve as a biomarker of AD, although the diagnostic value for individual patients is limited. An elevated CSF sLR11 level in AD patients may be relevant to AD pathogenesis.
Ikeuchi
Takeshi
T
Department of Molecular Neuroscience, Brain Research Institute, Niigata University, Niigata, Japan. ikeuchi@bri.niigata-u.ac.jp
Hirayama
Satoshi
S
Miida
Takashi
T
Fukamachi
Isamu
I
Tokutake
Takayoshi
T
Ebinuma
Hiroyuki
H
Takubo
Kohei
K
Kaneko
Hiroyuki
H
Kasuga
Kensaku
K
Kakita
Akiyoshi
A
Takahashi
Hitoshi
H
Bujo
Hideaki
H
Saito
Yasushi
Y
Nishizawa
Masatoyo
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics; D015415:Biomarkers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D057174:Frontotemporal Lobar Degeneration / Q000134:cerebrospinal fluid; D005838:Genotype; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000134:cerebrospinal fluid*; D008297:Male; D026901:Membrane Transport Proteins / Q000134:cerebrospinal fluid*; D008875:Middle Aged; D016875:tau Proteins / Q000134:cerebrospinal fluid
D001057:Apolipoproteins E; D015415:Biomarkers; D026502:LDL-Receptor Related Proteins; D026901:Membrane Transport Proteins; C102582:SORL1 protein, human; D016875:tau Proteins