Protein--nanoparticle interaction: identification of the ubiquitin--gold nanoparticle interaction site.
We demonstrate that it is possible to identify the protein--nanoparticle interaction site at amino acid scale in solution. Using NMR, chemical shift perturbation analysis, and dynamic light scattering we have identified a specific domain of human ubiquitin that interacts with gold nanoparticles. This method allows a detailed structural analysis of proteins absorbed onto surfaces of nanoparticles in physiological conditions and it will provide much needed experimental data for better modeling and prediction of protein--nanoparticle interactions.
Calzolai
Luigi
L
European Commission, Joint Research Centre, Institute for Health and Consumer Protection, I-21027 Ispra, Varese, Italy.
Franchini
Fabio
F
Gilliland
Douglas
D
Rossi
François
F
D001665:Binding Sites; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D006046:Gold