title: Preparative separation of six antimycin A components from antimycin fermentation broth by high-speed counter-current chromatography.
abstract: A method of using high-speed counter-current chromatography (HSCCC) was established for preparative isolation and purification of antimycin A components from antimycin fermentation broth. Six antimycin A components were successfully purified for the first time by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:2:4:1, by volume). Total of 20mg antimycin A(4)(a or b), 25mg antimycin A(3)(a or b), 21mg antimycin A(8)(a or b), 34mg antimycin A(2)(a or b), 26mg antimycin A(1)(a or b) and 34mg antimycin A(1)(a or b) with the purities of 93.2, 98.6, 96.2, 94.1, 94.9 and 96.7%, respectively, determined by high-performance liquid chromatography (HPLC), were yielded from 200mg crude sample only in one HSCCC run.
authors.affiliation: Fujian Medical University, Fuzhou, China.
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Wang
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Wen
authors.forename: Xiuming
authors.initials: X
authors.lastname: Chen
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Lin
authors.forename: Jingming
authors.initials: J
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Xie
authors.forename: Huaiwang
authors.initials: H
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000085:Acetates / Q000737:chemistry; D000968:Antimycin A / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D019149:Bioreactors; D003377:Countercurrent Distribution / Q000379:methods*; D017077:Culture Media, Conditioned / Q000737:chemistry; D005285:Fermentation; D006586:Hexanes / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D013302:Streptomyces / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000085:Acetates; D017077:Culture Media, Conditioned; D006586:Hexanes; D014867:Water; D000968:Antimycin A; C007650:ethyl acetate; D000432:Methanol