title: Plasma cortisol and 11-ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus.
abstract: Commercially available enzyme immunoassay (EIA) kits were validated for measuring steroid hormone concentrations in blood plasma from three fish species: the orange clownfish Amphiprion percula, the orangefin anemonefish Amphiprion chrysopterus and the blacktip reef shark Carcharhinus melanopterus. A minimum of 5 microl plasma was required to estimate hormone concentrations with both kits. These EIA kits are a simple method requiring minimal equipment, for measuring hormone profiles under field conditions.
authors.affiliation: USR 3278 CNRS-EPHE, CRIOBE-CBETM, Université de Perpignan, 66860 Perpignan Cedex, France. mills@sooozie.co.uk
authors.forename: S C
authors.initials: SC
authors.lastname: Mills
authors.forename: J
authors.initials: J
authors.lastname: Mourier
authors.forename: R
authors.initials: R
authors.lastname: Galzin
mesh_terms: D000818:Animals; D005260:Female; D006854:Hydrocortisone / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary*; D008297:Male; D010473:Perciformes / Q000097:blood / Q000502:physiology*; D012680:Sensitivity and Specificity; D012754:Sharks / Q000097:blood / Q000502:physiology*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D013739:Testosterone; C003600:11-ketotestosterone; D006854:Hydrocortisone