Microbial communities involved in electricity generation from sulfide oxidation in a microbial fuel cell.
Simultaneous electricity generation and sulfide removal can be achieved in a microbial fuel cell (MFC). In electricity harvesting from sulfide oxidation in such an MFC, various microbial communities are involved. It is essential to elucidate the microbial communities and their roles in the sulfide conversion and electricity generation. In this work, an MFC was constructed to enrich a microbial consortium, which could harvest electricity from sulfide oxidation. Electrochemical analysis demonstrated that microbial catalysis was involved in electricity output in the sulfide-fed MFC. The anode-attached and planktonic communities could perform catalysis independently, and synergistic interactions occurred when the two communities worked together. A 16S rRNA clone library analysis was employed to characterize the microbial communities in the MFC. The anode-attached and planktonic communities shared similar richness and diversity, while the LIBSHUFF analysis revealed that the two community structures were significantly different. The exoelectrogenic, sulfur-oxidizing and sulfate-reducing bacteria were found in the MFC anodic chamber. The discovery of these bacteria was consistent with the community characteristics for electricity generation from sulfide oxidation. The exoelectrogenic bacteria were found both on the anode and in the solution. The sulfur-oxidizing bacteria were present in greater abundance on the anode than in the solution, while the sulfate-reducing bacteria preferably lived in the solution.
Sun
Min
M
Department of Chemistry, University of Science & Technology of China, 96 Jinzhai Road, Hefei 230026, China.
Tong
Zhong-Hua
ZH
Sheng
Guo-Ping
GP
Chen
Yong-Zhen
YZ
Zhang
Feng
F
Mu
Zhe-Xuan
ZX
Wang
Hua-Lin
HL
Zeng
Raymond J
RJ
Liu
Xian-Wei
XW
Yu
Han-Qing
HQ
Wei
Li
L
Ma
Fang
F
D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004560:Electricity; D010084:Oxidation-Reduction; D020560:Proteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013045:Species Specificity; D013440:Sulfides / Q000378:metabolism*
D013440:Sulfides