title: Modulation of human neutrophil survival and antigen expression by activated CD4+ and CD8+ T cells.
abstract: Neutrophils and T cells often co-infiltrate pathological tissues, which suggests that these 2 cell types may interact with each other. Over the years, in vitro studies have demonstrated that neutrophils and T cells are capable of modulating each other's responses. However, few studies have examined the cross-talk between human neutrophils and CD8+ T cells, although murine models clearly show the critical role of their interaction in bacterial infections and cancer. Herein, we evaluated the interaction between human neutrophils and CD8+ T cells in a coculture system using highly purified cell preparations and compared the responses to ones from cocultures of neutrophils and CD4+ T cells. We report that anti-CD3-activated CD4+ and more potently, anti-CD3-activated CD8+ T cells modulate apoptosis and expression of activation markers by neutrophils, and neutrophils have no or little effect on T cell survival, expression of surface markers, and cytokine release. The observed effects of CD8+ T cells on neutrophils were mainly attributable to the release of TNF-α, IFN-γ, and GM-CSF, and the effects of CD4+ T cells involved IFN-γ and GM-CSF production. This study expands our knowledge of the molecular bases, whereby human CD4+ and CD8+ T cells modulate neutrophil survival and antigen expression.
authors.affiliation: Department of Pathology and Diagnostic, Section of General Pathology, University of Verona, Verona, Italy.
authors.forename: Martin
authors.initials: M
authors.lastname: Pelletier
authors.forename: Alessandra
authors.initials: A
authors.lastname: Micheletti
authors.forename: Marco A
authors.initials: MA
authors.lastname: Cassatella
mesh_terms: D017209:Apoptosis; D039481:CD11b Antigen / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D008213:Lymphocyte Activation; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D017452:Receptors, IgG / Q000032:analysis
chemical_list: D039481:CD11b Antigen; D016207:Cytokines; C509295:ITGAM protein, human; D017452:Receptors, IgG