Isolation and characterization of mesenchymal stem cells from human amniotic membrane.
BACKGROUND
The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy.


OBJECTIVE
To compare two previously published protocols for the isolation of human amnion mesenchymal stromal cells (hAMSCs), including their phenotypic characterization and in vitro potential for differentiation toward osteogenic, adipogenic, and chondrogenic mesodermal lineages.


METHODS
Human placentas were obtained from selected caesarean-sectioned births. Two different protocols (Alviano et al. (1) and Soncini et al. (2) ) for the isolation of hAMSCs were performed. After monolayer expansion of adherent cells from both protocols, the cells were characterized by flow cytometry and for multipotentiality, as assessed by their capability to differentiate toward adipocyte-, osteoblast-, and chondrocyte-like cells.


RESULTS
Both protocols yielded hAMSCs that showed plastic adherence, fibroblast-like growth, and well-defined human MSC markers. The cell yield and mesodermal differentiation capability of hAMSCs were higher in cells isolated using the Soncini protocol.


CONCLUSIONS
Our data demonstrated the successful isolation of hAMSCs from full-term placentas using two published protocols. Differences between the two protocols in cell yield and in vitro differentiation potential are shown.
Díaz-Prado
Silvia
S
1 Department of Medicine, INIBIC-University of A Coruña , A Coruña, Spain .
Muiños-López
Emma
E
Hermida-Gómez
Tamara
T
Rendal-Vázquez
Ma Esther
ME
Fuentes-Boquete
Isaac
I
de Toro
Francisco J
FJ
Blanco
Francisco J
FJ
D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis; D000650:Amnion / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D002585:Cesarean Section; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D010641:Phenotype; D010920:Placenta / Q000166:cytology* / Q000473:pathology; D011247:Pregnancy; D044968:Regenerative Medicine / Q000379:methods