title: Novel streptavidin-functionalized silicon nanowire arrays for CD4+ T lymphocyte separation.
abstract: Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over approximately 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
authors.affiliation: Global Research Laboratory, Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul 136-701, Korea.
authors.forename: Sung Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Dong-Joo
authors.initials: DJ
authors.lastname: Kim
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Kim
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Seo
authors.forename: Tae-Hong
authors.initials: TH
authors.lastname: Kim
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Lee
authors.forename: Kwanghee
authors.initials: K
authors.lastname: Kim
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Lee
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002469:Cell Separation; D051379:Mice; D053770:Nanowires; D012825:Silicon / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D019809:Streptavidin; D012825:Silicon