title: Phage-displayed peptides selected for binding to Campylobacter jejuni are antimicrobial.
abstract: In developed countries, Campylobacter jejuni is a leading cause of zoonotic bacterial gastroenteritis in humans with chicken meat implicated as a source of infection. Campylobacter jejuni colonises the lower gastrointestinal tract of poultry and during processing is spread from the gastrointestinal tract onto the surface of dressed carcasses. Controlling or eliminating C.jejuni on-farm is considered to be one of the best strategies for reducing human infection. Molecules on the cell surface of C.jejuni interact with the host to facilitate its colonisation and persistence in the gastrointestinal tract of poultry. We used a subtractive phage-display protocol to affinity select for peptides binding to the cell surface of a poultry isolate of C.jejuni with the aim of finding peptides that could be used to control this microorganism in chickens. In total, 27 phage peptides, representing 11 unique clones, were found to inhibit the growth of C.jejuni by up to 99.9% in vitro. One clone was bactericidal, reducing the viability of C.jejuni by 87% in vitro. The phage peptides were highly specific. They completely inhibited the growth of two of the four poultry isolates of C.jejuni tested with no activity detected towards other Gram-negative and Gram-positive bacteria.
authors.affiliation: CSIRO-Livestock Industries, Queensland Biosciences Precinct, 306 Carmody Road, St. Lucia, QLD 4067, Australia. Sharon.Bishop-Hurley@csiro.au
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Bishop-Hurley
authors.forename: Philippa J
authors.initials: PJ
authors.lastname: Rea
authors.forename: Christopher S
authors.initials: CS
authors.lastname: McSweeney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D001435:Bacteriophages / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology; D016123:Campylobacter jejuni / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002645:Chickens; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011200:Poultry / Q000382:microbiology*
chemical_list: D000890:Anti-Infective Agents; D019151:Peptide Library; D010455:Peptides