The structure of the O-antigen of Cronobacter sakazakii HPB 2855 isolate involved in a neonatal infection.
Strains of Cronobactersakazakii (previously known as Enterobactersakazakii) are medically recognized important Gram-negative bacterial pathogens that cause enterocolitis, septicemia, and meningitis, with a high mortality rate in neonates. The structure of their O-antigens, that form part of their somatic lipopolysaccharide (LPS) components, is of interest for their chemical and serological identification and their relationship to virulence. The O-polysaccharide (O-PS) of C.sakazakii HPB 2855 (SK 81), a strain isolated from an infant at the Hospital for Sick Children in Toronto in 1981, was shown to be a polymer of a partially O-acetylated-repeating hexasaccharide unit composed of d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-galactose, and l-rhamnose (1:1:1:3). From composition and methylation analysis, and the application of 1D and 2D (1)H and (13)C NMR spectroscopy, the O-PS was determined to be a polymer of a repeating oligosaccharide unit having the structure:
Maclean
Leann L
LL
Institute for Biological Sciences, National Research Council, Ottawa, Canada.
Vinogradov
Evgeny
E
Pagotto
Franco
F
Farber
Jeffrey M
JM
Perry
Malcolm B
MB
D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
D019081:O Antigens