Detection of C-reactive protein in evanescent wave field using microparticle-tracking velocimetry.
A new technique is developed to measure the nanoparticles' brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles' movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 microg/mL even in a high-viscosity solution. Further, the dissociation constant, K(D), can be evaluated based on the experimental results.
Fan
Yu-Jui
YJ
Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan.
Sheen
Horn-Jiunn
HJ
Liu
Yi-Hsing
YH
Tsai
Jing-Fa
JF
Wu
Tzu-Heng
TH
Wu
Kuang-Chong
KC
Lin
Shiming
S
D002097:C-Reactive Protein / Q000032:analysis*; D053758:Nanoparticles / Q000032:analysis*; D012212:Rheology / Q000379:methods*; D013050:Spectrometry, Fluorescence; D014783:Viscosity
D002097:C-Reactive Protein