Pediatric measles vaccine expressing a dengue tetravalent antigen elicits neutralizing antibodies against all four dengue viruses.
Dengue disease is an increasing global health problem that threatens one-third of the world's population. To control this emerging arbovirus, an efficient preventive vaccine is still needed. Because four serotypes of dengue virus (DV) coexist and antibody-dependent enhanced infection may occur, most strategies developed so far rely on the administration of tetravalent formulations of four live attenuated or chimeric viruses. Here, we evaluated a new strategy based on the expression of a single minimal tetravalent DV antigen by a single replicating viral vector derived from pediatric live-attenuated measles vaccine (MV). We generated a recombinant MV vector expressing a DV construct composed of the four envelope domain III (EDIII) from the four DV serotypes fused with the ectodomain of the membrane protein (ectoM). After two injections in mice susceptible to MV infection, the recombinant vector induced neutralizing antibodies against the four serotypes of dengue virus. When immunized mice were further inoculated with live DV from each serotype, a strong memory neutralizing response was raised against all four serotypes. A combined measles-dengue vaccine might be attractive to immunize infants against both diseases where they co-exist.
Brandler
Samantha
S
Viral Genomics and Vaccination Unit, Institut Pasteur, CNRS-URA3015, 28 rue du Dr Roux, 75015 Paris, France.
Ruffie
Claude
C
Najburg
Valérie
V
Frenkiel
Marie-Pascale
MP
Bedouelle
Hughes
H
Desprès
Philippe
P
Tangy
Frédéric
F
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D002522:Chlorocebus aethiops; D003715:Dengue / Q000276:immunology / Q000517:prevention & control*; D053059:Dengue Vaccines / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology; D004330:Drosophila / Q000166:cytology; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D056724:Immunity, Humoral; D007156:Immunologic Memory; D008297:Male; D008458:Measles Vaccine / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D009500:Neutralization Tests; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D053059:Dengue Vaccines; D008458:Measles Vaccine; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins