title: Serum and urinary metabonomic study of human osteosarcoma.
abstract: Osteosarcoma (OS) is the most common malignant bone tumor found in children. Currently, researchers have focused on protein and gene levels, while the associated metabolic variations have been poorly understood. In this study, we used a gas chromatography mass spectrometry approach and profiled small-molecule metabolites in serum and urine of 24 OS patients, 19 benign bone tumor patients, and 32 healthy controls, to determine whether there are significant alterations associated with carcinogenesis. The metabonomic results demonstrate clear intergroup separations between healthy control subjects and bone tumor patients in the orthogonal partial least-squares-discriminant analysis (OPLS-DA) models. Differential metabolites identified from the metabonomic analysis suggest a disrupted energy metabolism in OS patients, as characterized by significantly down-regulated TCA cycle and glycolysis, down-regulated lipid metabolism, dysregulated sugar levels, and up-regulated amino acid metabolism. Additionally, an increased activity of glutathione metabolism, and increased polyamine metabolism also contributed to a characteristic metabolic signature of OS patients.
authors.affiliation: Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai 200072, PR China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yunping
authors.initials: Y
authors.lastname: Qiu
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Hua
authors.forename: Yihuang
authors.initials: Y
authors.lastname: Wang
authors.forename: Tianlu
authors.initials: T
authors.lastname: Chen
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Chi
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Shuo
authors.initials: S
authors.lastname: Hu
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Chengwei
authors.initials: C
authors.lastname: Yang
authors.forename: Guodong
authors.initials: G
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000097:blood / Q000652:urine; D014408:Biomarkers, Tumor / Q000097:blood / Q000652:urine; D001859:Bone Neoplasms / Q000097:blood* / Q000652:urine*; D016022:Case-Control Studies; D016002:Discriminant Analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006626:Hippurates / Q000097:blood / Q000652:urine; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D012516:Osteosarcoma / Q000097:blood* / Q000652:urine*; D025341:Principal Component Analysis; D011700:Putrescine / Q000097:blood / Q000652:urine
chemical_list: D000596:Amino Acids; D014408:Biomarkers, Tumor; D006626:Hippurates; C030514:hippuric acid; D011700:Putrescine