title: Molecular characterization of rotavirus strains circulating among children with acute gastroenteritis in Madagascar during 2004-2005.
abstract: A survey was undertaken of the etiology of acute gastroenteritis in children <16 years of age in Antananarivo, Madagascar, from May 2004 through May 2005. With use of electron microscopy of fecal specimens, 104 (36%) of 285 children were found to be infected with rotavirus. Rotavirus strain characterization was undertaken using enzyme-linked immunosorbent assay, electropherotyping, reverse-transcription polymerase chain reaction genotyping, and nucleotide sequencing. The predominant group A rotavirus strain types identified were P[4]G2 (62%) and P[8]G9 (23%). Nucleotide sequence analysis of the VP7 genes of selected Malagasy G2 and G9 strains demonstrated similarity with those of other recently identified African rotavirus strains belonging to the same genotype.
authors.affiliation: University of Liverpool, United Kingdom.
authors.forename: T K
authors.initials: TK
authors.lastname: Adiku
authors.forename: W
authors.initials: W
authors.lastname: Dove
authors.forename: P
authors.initials: P
authors.lastname: Grosjean
authors.forename: P
authors.initials: P
authors.lastname: Combe
authors.forename: T
authors.initials: T
authors.lastname: Nakagomi
authors.forename: O
authors.initials: O
authors.lastname: Nakagomi
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
authors.forename: N A
authors.initials: NA
authors.lastname: Cunliffe
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008270:Madagascar / Q000453:epidemiology; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*