Glucose metabolism in mammalian cell culture: new insights for tweaking vintage pathways.
Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.
Mulukutla
Bhanu Chandra
BC
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA.
Khan
Salmaan
S
Lange
Alex
A
Hu
Wei-Shou
WS
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D053858:Metabolic Networks and Pathways; D015398:Signal Transduction; D049490:Systems Biology / Q000379:methods*
D005947:Glucose