title: Resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in rolC transgenic cell cultures of Vitis amurensis.
abstract: Resveratrol, a naturally occurring polyphenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. In the present investigation, we show that transformation of Vitis amurensis Rupr. with the oncogene rolC of Agrobacterium rhizogenes increased resveratrol production in the two transformed callus cultures 3.7 and 11.9 times. The rolC-transformed calli were capable of producing 0.099% and 0.144% dry weight of resveratrol. We characterized phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) gene expression in the two rolC transgenic callus cultures of V. amurensis. In the rolC transgenic culture with higher resveratrol content, expression of VaPAL3, VaSTS3, VaSTS4, VaSTS5, VaSTS6, VaSTS8, VaSTS9, and VaSTS10 was increased; while in the rolC culture with lower resveratrol content, expression of VaPAL3 and VaSTS9 was increased. We suggest that transformation of V. amurensis calli with the rolС gene induced resveratrol accumulation via selective enhancement of expression of individual PAL and STS genes involved in resveratrol biosynthesis. We compared the data on PAL and STS gene expression in rolC transgenic calli with the previously obtained results for rolB transgenic calli of V. amurensis. We propose that the transformation of V. amurensis with the rolC and rolB genes of A. rhizogenes increased resveratrol accumulation through different regulatory pathways.
authors.affiliation: Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia.
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Dubrovina
authors.forename: Artem Y
authors.initials: AY
authors.lastname: Manyakhin
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Zhuravlev
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Kiselev
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant; D005798:Genes, Bacterial; D005810:Multigene Family; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D000077185:Resveratrol; D012231:Rhizobium / Q000235:genetics; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000201:enzymology* / Q000235:genetics
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; D010650:Phenylalanine Ammonia-Lyase; D000077185:Resveratrol