title: Mass spectrometry of murine leukemia virus core proteins.
abstract: A mass spectrometry (MS) approach was used to analyze viral core proteins of the murine leukemia virus (MuLV)-based gene delivery vector. The retroviral particles produced by traditional methods were concentrated and purified by ultracentrifugation and spin column for matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) MS. MALDI application detected all core MuLV proteins, partial degradation of p10, phosphorylation of p12, as well as the previously unknown formation of a polymeric supramolecular complex between p15 and p30 core proteins. ESI provided information on the post-translational modifications of MuLV core proteins. Data suggest myristoylation of p15 and oxidation of methionine residues in both p12 and p30, whereas cysteine residues in p10, p15 and p30 were not oxidized. The current study demonstrates that MALDI and ESI are efficient tools for viral core protein analysis and can be used as analytical tools in virology and biotechnology of gene delivery vectors.
authors.affiliation: Gene Therapy Laboratories, University of Southern California School of Medicine, 1441 Eastlake Avenue, 612, Los Angeles, CA 90033, USA.
authors.forename: Viacheslav
authors.initials: V
authors.lastname: Medvedkin
authors.forename: Roger
authors.initials: R
authors.lastname: Moore
authors.forename: Arnold M
authors.initials: AM
authors.lastname: Falick
authors.forename: John
authors.initials: J
authors.lastname: Conner
authors.forename: Tatiana
authors.initials: T
authors.lastname: Omelchenko
authors.forename: Mary
authors.initials: M
authors.lastname: Young
authors.forename: W French
authors.initials: WF
authors.lastname: Anderson
authors.forename: Vladislav S
authors.initials: VS
authors.lastname: Golubkov
authors.forename: Yanina
authors.initials: Y
authors.lastname: Rozenberg-Adler
mesh_terms: D009052:Leukemia Virus, Murine / Q000737:chemistry* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014461:Ultracentrifugation; D014758:Viral Core Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014758:Viral Core Proteins