Identification of new pathogens in the intraocular fluid of patients with uveitis.
OBJECTIVE
To determine infectious causes in patients with uveitis of unknown origin by intraocular fluids analysis.


METHODS
Case-control study.


METHODS
Ocular fluids from 139 patients suspected of infectious uveitis, but negative for herpes simplex virus, varicella-zoster virus, cytomegalovirus, and Toxoplasma gondii by polymerase chain reaction and/or antibody analysis in intraocular fluids, were assessed for the presence of 18 viruses and 3 bacteria by real-time polymerase chain reaction (PCR). The ocular fluids from 48 patients with uveitis of known etiology or with cataract were included as controls.


RESULTS
Positive PCR results were found for Epstein-Barr virus, for rubella virus, and for human herpesvirus 6 each in 1 patient and for human parechovirus in 4 patients. Of the human parechovirus-positive patients, 1 was immunocompromised and had panuveitis. The other 3 patients were immunocompetent and had anterior uveitis, all with corneal involvement.


CONCLUSIONS
Human parechovirus might be associated with infectious (kerato)uveitis.
de Groot-Mijnes
Jolanda D F
JD
Department of Virology, University Medical Center Utrecht, The Netherlands. J.D.FdeGroot@umcutrecht.nl
de Visser
Lenneke
L
Zuurveen
Stephanie
S
Martinus
Roaldy A
RA
Völker
René
R
ten Dam-van Loon
Ninette H
NH
de Boer
Joke H
JH
Postma
Gina
G
de Groot
Raoul J
RJ
van Loon
Anton M
AM
Rothova
Aniki
A
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001082:Aqueous Humor / Q000821:virology*; D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000821:virology; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000821:virology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D030061:Parechovirus / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000821:virology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D019349:Roseolovirus Infections / Q000175:diagnosis / Q000821:virology; D012409:Rubella / Q000175:diagnosis / Q000821:virology; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*; D014606:Uveitis, Anterior / Q000821:virology*
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral