Microevolution of Mycobacterium tuberculosis in a tuberculosis patient.
Five Mycobacterium tuberculosis isolates were obtained from three body sites from a Dutch patient. The isolates displayed a single genotype by 24-locus MIRU-VNTR typing (except for a single locus not amplified from one isolate) but were differentiated by small variations in IS6110 fingerprints, spoligotypes, 6 hypervariable MIRU-VNTR loci, and/or DiversiLab profiles, revealing patterns of microevolution in a clonal infection.
Al-Hajoj
Sahal A M
SA
Department of Comparative Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. hajoj@kfshrc.edu.sa
Akkerman
Onno
O
Parwati
Ida
I
al-Gamdi
Saad
S
Rahim
Zeaur
Z
van Soolingen
Dick
D
van Ingen
Jakko
J
Supply
Philip
P
van der Zanden
Adri G M
AG
D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009426:Netherlands; D014376:Tuberculosis / Q000382:microbiology*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial