title: [Comparative thermodynamic analysis of thrombin interaction with anti-thrombin aptamers and their heterodimeric construct].
abstract: Aptamers interacting selectively with the anion-binding exosites 1 and 2 of thrombin were merged into dimeric oligonucleotide constructs with use of a poly-(dT)-linker of 35 nucleotides (nt) long. Complexes of thrombin with the aptamers and their hetero- and homodimeric constructs were measured using the optical biosensor Biacore-3000. K(D) values measured for the hetero- and homodimeric constructs were correspondingly 25-30- and 2-3-fold lower than those for the primary aptamers. Analysis of temperature dependencies of K(D) values within the temperature interval of 10 degrees C-40 degrees C has shown that the values of enthalpy change deltaH upon formation of complexes of thrombin with the aptamers and the hetrodimeric construct are close. The value of the entropy change deltaS upon complex formation of thrombin with the aptamer heterodimeric construct was 1.5-2-fold higher than deltaS values for the complexes with the aptamers. The complex formation and dissociation rates increased with the elevation of temperature from 10 degrees C to 37 degrees C. However, the dissociation rate for the complex of thrombin with the heterodimeric construct was evidently lower that that for the complexes with the aptamers.
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Rakhmetova
authors.forename: S P
authors.initials: SP
authors.lastname: Rad'ko
authors.forename: O V
authors.initials: OV
authors.lastname: Gnedenko
authors.forename: N V
authors.initials: NV
authors.lastname: Bodoev
authors.forename: A S
authors.initials: AS
authors.lastname: Ivanov
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D019281:Dimerization; D013696:Temperature; D013816:Thermodynamics; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin