title: Chemical vectors for gene delivery: uptake and intracellular trafficking.
abstract: Chemical vectors for non-viral gene delivery are based on engineered DNA nanoparticles produced with various range of macromolecules suitable to mimic some viral functions required for gene transfer. Many efforts have been undertaken these past years to identify cellular barriers that have to be passed for this issue. Here, we summarize the current status of knowledge on the uptake mechanism of DNA nanoparticles made with polymers and liposomes, their endosomal escape, cytosolic diffusion, and nuclear import of pDNA. Studies reported these past years regarding pDNA nanoparticles endocytosis indicated that there is no clear evident relationship between the ways of entry and the transfection efficiency. By contrast, the sequestration of pDNA in intracellular vesicles and the low number of pDNA close to the nuclear envelop are identified as the major intracellular barriers. So, intensive investigations to increase the cytosolic delivery of pDNA and its migration toward nuclear pores make sense to bring the transfection efficiency closer to that of viruses.
authors.affiliation: Centre de Biophysique Moléculaire CNRS UPR 4301, University of Orléans and Inserm, rue Charles Sadron, 45071 Orléans Cedex 2, France. chantal.pichon@cnrs-orleans.fr
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
authors.forename: Ludivine
authors.initials: L
authors.lastname: Billiet
authors.forename: Patrick
authors.initials: P
authors.lastname: Midoux
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry* / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D011108:Polymers / Q000737:chemistry / Q000378:metabolism
chemical_list: D008081:Liposomes; D011108:Polymers