[Effect of liposomal transfection of UCH-L1 siRNA on proliferation and apoptosis of lung cancer cell line H157].
OBJECTIVE
Several reports demonstrated that the ubiquitin C terminal hydrolase-L1 (UCH-L1) has been found to be an oncogene in malignant tumors such as esophageal carcinoma, lung cancer and breast cancer. The aim of this study is to explore the effects of liposomal transfection of UCH-L1 siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines H157.


METHODS
UCH-L1 siRNA was synthesized and transfected into H157 cell by liposome. Cell morphological change was observed with microscope, and cell proliferation and apoptosis index detected by flow cytometry, UCH-L1 mRNA expression was determined by RT-PCR and protein level of UCH-L1 was determined by Western blot.


RESULTS
For the H157 cell transfected with siRNA, cell proliferation was inhibited significantly, cell apoptosis appeared obviously, the expression of UCH-L1 mRNA and protein level of UCH-L1 significantly decreased.


CONCLUSIONS
UCH-L1 siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines H157 and induce the apoptosis. UCH-L1 might become a new target for lung carcinoma gene therapy.
Qu
Xiaojie
X
Department of Respiratory Medicine, 1st Hospital Affiliated to Dalian Medical University, Dalian 116011, China.
Wang
Yanfu
Y
D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008175:Lung Neoplasms / Q000628:therapy*; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D043222:Ubiquitin Thiolesterase / Q000235:genetics*
D008081:Liposomes; D034741:RNA, Small Interfering; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase