title: Genetic fate-mapping for studying adult cardiomyocyte replenishment after myocardial injury.
abstract: Mounting evidence suggests the regenerative potential of the mammalian heart. Nevertheless, the contribution of endogenous stem or precursor cells to adult cardiac regeneration upon myocardial injuries remains unclear. We hereby describe a genetic fate-mapping approach to study adult cardiomyocyte replenishment after myocardial injury. Using double transgenic MerCreMer-ZEG mice, the fate of adult cardiomyocytes can be tracked by the expression of green fluorescence protein (GFP) specifically induced in cardiomyocytes. Upon experimental myocardial infarction, a reduction in GFP expression in the myocardium is observed, indicating the refreshment of cardiomyocytes by endogenous stem or precursor cells.
authors.affiliation: Graduate Institute of Clinical Medicine and Research Center for Clinical Medicine, National Cheng Kung University & Hospital, Tainan City, Taiwan.
authors.forename: Sunny S-K
authors.initials: SS
authors.lastname: Chan
authors.forename: Ying-Zhang
authors.initials: YZ
authors.lastname: Shueh
authors.forename: Nirma
authors.initials: N
authors.lastname: Bustamante
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Tsai
authors.forename: Hua-Lin
authors.initials: HL
authors.lastname: Wu
authors.forename: Jyh-Hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Patrick C H
authors.initials: PC
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D016133:Polymerase Chain Reaction
chemical_list: D049452:Green Fluorescent Proteins