title: Black-tea polyphenols decrease micellar solubility of cholesterol in vitro and intestinal absorption of cholesterol in rats.
abstract: Administration of black-tea polyphenols (BTP) simultaneously reduced lymphatic recovery of both (3)H-cholesterol and (14)C-trioleoylglycerol in rats that were cannulated in the thoracic duct. BTP decreased the in vitro micellar solubility of cholesterol in a dose-dependent manner. When purified theaflavins, which are components of BTP, were used, theaflavin-monogallates (TFMGs), theaflavin-3-gallate (TF3G), and theaflavin-3'-gallate (TF3'G) were effective in eliminating cholesterol from bile salt micelles in vitro. Theaflavin (TF) and theaflavin-3,3'-digallate (TFDG) had no effect on the micellar solubility of cholesterol. The concentration of bile acid in the micelles was not influenced by the addition of any BTPs or theaflavins. These results suggest that the reduction of micellar cholesterol by BTP could be important to reducing cholesterol absorption.
authors.affiliation: Laboratory of Food and Biomolecular Science, Department of Food Function and Health, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan. iikeda@biochem.tohoku.ac.jp
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ikeda
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamahira
authors.forename: Masaki
authors.initials: M
authors.lastname: Kato
authors.forename: Ayako
authors.initials: A
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000008:administration & dosage*; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D008823:Micelles; D023421:Models, Animal; D008954:Models, Biological; D010636:Phenols / Q000008:administration & dosage*; D010936:Plant Extracts / Q000008:administration & dosage*; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013897:Thoracic Duct / Q000187:drug effects / Q000378:metabolism
chemical_list: D005419:Flavonoids; D008823:Micelles; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D002784:Cholesterol