Extraction, chemical analysis of Angelica sinensis polysaccharides and antioxidant activity of the polysaccharides in ischemia-reperfusion rats.
Angelica sinensis polysaccharides were analyzed using high performance liquid chromatography (HPLC) and Fourier Transform Infrared (FT-IR). The major sugar of the polysaccharide was saccharose (18.55%); and the sugar constituted about 83% of the monomer content. Glucose and fructose were found as minor components of the polysaccharides. The FT-IR spectra of A. sinensis polysaccharides are used for determination of their structural features. The FT-IR spectrum of A. sinensis polysaccharides showed bands at 1641 cm(-1), 1415 cm(-1), 1050 cm(-1) and 926 cm(-1) characteristic for the carboxylic group. Absorptions at 2920-2930 cm(-1) are attributed to asymmetrical stretching vibration of CH(2)-group. Medium stretch observed in the range 1650-1400 cm(-1) is assigned to C-C stretching of polysaccharides. Cardioprotective effects of A. sinensis polysaccharides were evaluated by using myocardial ischemia/reperfusion (IR) rats. A. sinensis polysaccharides treatment significantly reduced myocardial infarction size, enhanced CT-1 and antioxidant enzymes activity, downregulated caspase-12 mRNA expression in rats. The study strongly suggests the cardioprotective activity of A. sinensis polysaccharides in limiting ischemia-reperfusion induced myocardial injury.
Zhang
Song
S
Department of Cardiovascular Diseases, Eastern District of Renji Hospital, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China.
He
Ben
B
Ge
Junbo
J
Li
Huibin
H
Luo
Xiuying
X
Zhang
Hui
H
Li
Yuhui
Y
Zhai
Changlin
C
Liu
Pingang
P
Liu
Xin
X
Fei
Xuetao
X
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D053454:Caspase 12 / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D008297:Male; D009203:Myocardial Infarction / Q000097:blood / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000150:complications / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000097:blood
D000975:Antioxidants; D016207:Cytokines; D011134:Polysaccharides; D012333:RNA, Messenger; C092073:cardiotrophin 1; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D053454:Caspase 12; D005978:Glutathione