[Effects of artemisinin on proliferation, apoptosis and Caspase-3 expression of rat mesangial cell].
OBJECTIVE
To study the effects of artemisinin on proliferation, apoptosis and Caspase-3 active of rat mesangial cell.


METHODS
Rat mesangial cells were incubated with different concentrations of artemisinin, the proliferation, apoptosis and Caspase-3 active of rat mesangial cell were measured by MTT assay, fluorescent inverted microscope and enzyme-labeled analytical instruments respectively.


RESULTS
Compared with control group, the proliferation and Caspase-3 expression of mesangial cell of three other groups were significantly different (P < 0.01). Compared with dexamethasone group, there were significant difference effects of proliferation and Caspase-3 expression of mesangial cell in other two groups of identical concentration drugs (P < 0. 01), especially in the artemisinin + glucocorticoid (ArtGC) group, and the effects of three different drugs on the mesangial cell Caspase-3 expression, proliferation and apoptosis had the tendency of depend on dosage, and mass mortality of mesangial cell in the mediate-dosage and high-dosage ArtGC group.


CONCLUSIONS
Artemisinin could inhibit the proliferation of mesangial cell, enhance the expression of Caspase-3 and promote the apoptosis in a dose-dependent manner.
Wu
Xi-Li
XL
Department of integrated Chinese Traditional and Western Medicine, Shanxi Province Key TCM Department of Nephrology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China. wuxili1984@163.com
Zhang
Wang-Gang
WG
Shi
Xing-Min
XM
An
Peng
P
Sun
Wan-Sen
WS
Qiao
Cheng-Lin
CL
Wang
Zhu
Z
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019306:Artemisia / Q000737:chemistry; D037621:Artemisinins / Q000008:administration & dosage / Q000494:pharmacology*; D001623:Betamethasone / Q000008:administration & dosage / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D001623:Betamethasone; C031327:artemisinin; C505410:Casp3 protein, rat; D053148:Caspase 3