title: The chemical constituents of Piper kadsura and their cytotoxic and anti-neuroinflammtaory activities.
abstract: The n-hexane and CHCl₃ soluble fractions of the MeOH extract of the aerial parts of Piper kadsura were found to potently inhibit nitric oxide (NO) production in LPS-activated BV-2 cells, a microglial cell line. From the active fractions, a new stereoisomer of guaiane sesquiterpene, 1α,5β-guai-4(15)-ene-6β,10β-diol, kadsuguain A (1) and a new cyclohexadienone, kadsuketanone A (2), together with twelve known compounds (3-14) were isolated. The structures of these compounds were elucidated by extensive NMR spectral studies. The absolute configuration of 2 was determined by circular dichroism (CD) spectra. Compounds 2, 6, and 11-14 significantly inhibited both nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in the LPS-activated microglia cells. In addition, compounds 4, 6, and 11-14 exhibited cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT15 human tumour cells.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Jung Wook
authors.initials: JW
authors.lastname: Choi
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Sang Keun
authors.initials: SK
authors.lastname: Ha
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D015213:Neuroimmunomodulation / Q000187:drug effects*; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts