title: Primary effusion lymphoma: genomic profiling revealed amplification of SELPLG and CORO1C encoding for proteins important for cell migration.
abstract: Primary effusion lymphoma (PEL) is associated with Kaposi sarcoma herpesvirus (KSHV) but its pathogenesis is poorly understood. Many KSHV-associated products can deregulate cellular pathways commonly targeted in cancer. However, KSHV infection alone is insufficient for malignant transformation. PEL also lacks the chromosomal translocations seen in other lymphoma subtypes. We investigated 28 PELs and ten PEL cell lines by 1 Mb resolution array comparative genomic hybridization (CGH) and found frequent gains of 1q21-41 (47%), 4q28.3-35 (29%), 7q (58%), 8q (63%), 11 (32%), 12 (61%), 17q (29%), 19p (34%), and 20q (34%), and losses of 4q (32%), 11q25 (29%), and 14q32 (63%). Recurrent focal amplification was seen at several regions on chromosomes 7, 8, and 12. High-resolution chromosome-specific tile-path array CGH confirmed these findings, and identified selectin-P ligand (SELPLG) and coronin-1C (CORO1C) as the targets of a cryptic amplification at 12q24.11. Interphase FISH and quantitative PCR showed SELPLG/CORO1C amplification (>4 extra copies) and low levels of copy number gain (1-4 extra copies) in 23% of PELs, respectively. Immunohistochemistry revealed strong expression of both SELPLG and coronin-1C in the majority of PELs, irrespective of their gene dosage. SELPLG is critical for cell migration and chemotaxis, while CORO1C regulates actin-dependent processes, thus important for cell motility. Their overexpression in PEL is expected to play an important role in its pathogenesis.
authors.affiliation: Department of Pathology, University of Cambridge, Cambridge, CB2 0QQ, UK.
authors.forename: Shi-Lu
authors.initials: SL
authors.lastname: Luan
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Boulanger
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ye
authors.forename: Estelle
authors.initials: E
authors.lastname: Chanudet
authors.forename: Nicola
authors.initials: N
authors.lastname: Johnson
authors.forename: Rifat A
authors.initials: RA
authors.lastname: Hamoudi
authors.forename: Chris M
authors.initials: CM
authors.lastname: Bacon
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Liu
authors.forename: Yuanxue
authors.initials: Y
authors.lastname: Huang
authors.forename: Jonathan
authors.initials: J
authors.lastname: Said
authors.forename: Peiguo
authors.initials: P
authors.lastname: Chu
authors.forename: Christoph S
authors.initials: CS
authors.lastname: Clemen
authors.forename: Ethel
authors.initials: E
authors.lastname: Cesarman
authors.forename: Amy
authors.initials: A
authors.lastname: Chadburn
authors.forename: Peter G
authors.initials: PG
authors.lastname: Isaacson
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Du
mesh_terms: D002465:Cell Movement / Q000235:genetics; D002869:Chromosome Aberrations; D003043:Cocarcinogenesis; D055028:Comparative Genomic Hybridization / Q000379:methods; D020031:Epstein-Barr Virus Infections / Q000150:complications; D005784:Gene Amplification; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D054685:Lymphoma, Primary Effusion / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; C084882:P-selectin ligand protein; C072061:coronin proteins