Heterogeneity of radiation- and TNF-alpha-induced programmed cell death in carcinoma, sarcoma and lymphoma cell lines.
OBJECTIVE
To characterize the interaction of tumour necrosis factor alpha (TNF-alpha) and ionising radiation in six sarcoma, lymphoma and carcinoma cell lines.


METHODS
Cells were characterized regarding annexin V/propiduim iodine affinity and caspase-3 status after application of TNF-alpha, radiation, or combined treatment.


RESULTS
Three cell lines showed similar results with additive effects of TNF-alpha and radiation in both assays. The other three cell lines significantly differed regarding the detection of apoptotic cells between treatment conditions (radiation and/or TNF application) and between the two apoptosis assays.


CONCLUSIONS
The interaction between TNF-alpha and radiation differs between tumour entities and cannot be described by using only one parameter.
Bölling
Tobias
T
Department of Radiotherapy, University Hospital Münster, Albert-Schweitzer-Str. 33, 48149 Münster, Germany. Tobias.Boelling@uni-muenster.de
Kolkmeyer
Astrid
A
Greve
Burkhard
B
Ernst
Iris
I
Willich
Normann
N
Könemann
Stefan
S
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D002277:Carcinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D012509:Sarcoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D017304:Annexin A5; D014409:Tumor Necrosis Factor-alpha; D053148:Caspase 3