title: Halomonas daqiaonensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from a littoral saltern.
abstract: Two novel gram-negative, oxidase- and catalase-positive, rod-shaped bacterial strains, designated YCSA28(T) and YCSA39, were isolated from sediment of Daqiao saltern, Jimo, Qingdao, on the east coast of China. The two strains grew optimally at 28-30 °C, at pH 7.5 and in the presence of 7-8 % (w/v) NaCl. They were assigned to the genus Halomonas, class Gammaproteobacteria, based on 16S rRNA gene sequence analysis. The major cellular fatty acids of the two strains were C(18 : 1)ω7c (42.9 %), C(16 : 0) (23.1 %) and C(16 : 1)ω7c/ω6c (18.0 %), and Q-9 was the major ubiquinone. The G+C content of the DNA of strains YCSA28(T) and YCSA39 was 63.7 and 63.9 mol%, respectively. The predominant respiratory lipoquinone, cellular fatty acid profiles and DNA G+C content of strains YCSA28(T) and YCSA39 were consistent with those of recognized species of the genus Halomonas. Levels of DNA-DNA relatedness between strains YCSA28(T) and YCSA39, between YCSA28(T) and Halomonas ventosae Al12(T), and between YCSA39 and H. ventosae Al12(T) were 95, 45 and 50 %, respectively. Together, these data indicated that strains YCSA28(T) and YCSA39 represent a single novel species of the genus Halomonas, for which the name Halomonas daqiaonensis sp. nov. is proposed. The type strain is YCSA28(T) ( = CGMCC 1.9150(T)  = NCCB 100305(T)  = MCCC 1B00920(T)).
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Qu
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Zhu
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Hong
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
authors.affiliation: First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, PR China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Sun
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D058440:Denitrification; D005227:Fatty Acids / Q000737:chemistry; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D014451:Ubiquinone / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride; C030571:ubiquinone 9