title: Aqueous penetration of moxifloxacin 0.5% ophthalmic solution and besifloxacin 0.6% ophthalmic suspension in cataract surgery patients.
abstract: OBJECTIVE
  To determine the aqueous humor concentrations of moxifloxacin and besifloxacin after routine preoperative topical dosing in patients having cataract surgery.
  
  
  METHODS
  Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland, USA.
  
  
  METHODS
  In this prospective randomized parallel double-masked clinical trial, 1 drop of commercially available moxifloxacin 0.5% ophthalmic solution or besifloxacin 0.6% ophthalmic suspension was administered every 10 minutes for a total of 4 doses beginning 1 hour before routine cataract surgery. Aqueous humor was sampled via the paracentesis, and antibiotic concentrations were determined using validated high-performance liquid chromatography procedures.
  
  
  RESULTS
  The study enrolled 50 patients. The aqueous concentration of the antibiotic agent was detectable in all 23 moxifloxacin samples and in 10 (40%) of the 25 besifloxacin samples (P<.0001, Pearson chi-square test). The mean aqueous concentration in the moxifloxacin samples was 50-fold higher than in the besifloxacin samples (1.6108 microg/mL versus 0.0319 microg/mL) when all samples were included (P<.0001, Wilcoxon test), while the moxifloxacin concentration was 38-fold higher than the besifloxacin concentration (1.6108 microg/mL versus 0.0422 microg/mL) in the samples with detectable antibiotic agent (P<.0001).
  
  
  CONCLUSIONS
  After topical preoperative administration, moxifloxacin 0.5% ophthalmic solution had a 38-fold to 50-fold higher concentration in the aqueous humor than besifloxacin 0.6% ophthalmic suspension. Besifloxacin was undetectable in more than half the aqueous humor samples.
authors.affiliation: Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland 21287, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Yoshida
authors.forename: Alisa
authors.initials: A
authors.lastname: Kim
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Pratzer
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Stark
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D001082:Aqueous Humor / Q000378:metabolism*; D001372:Aza Compounds / Q000493:pharmacokinetics*; D001381:Azepines / Q000493:pharmacokinetics*; D002387:Cataract Extraction; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000378:metabolism; D004311:Double-Blind Method; D024841:Fluoroquinolones / Q000493:pharmacokinetics*; D006801:Humans; D008875:Middle Aged; D000077266:Moxifloxacin; D009883:Ophthalmic Solutions / Q000493:pharmacokinetics*; D011446:Prospective Studies; D011804:Quinolines / Q000493:pharmacokinetics*
chemical_list: D000890:Anti-Infective Agents; D001372:Aza Compounds; D001381:Azepines; D024841:Fluoroquinolones; D009883:Ophthalmic Solutions; D011804:Quinolines; C522124:besifloxacin; D000077266:Moxifloxacin