title: Matrix Gla protein expression in NRK-52E cells exposed to oxalate and calcium oxalate monohydrate crystals.
abstract: OBJECTIVE
  Matrix Gla protein (MGP), a potent calcification inhibitor in extracellular matrix, is a molecular determinant regulating vascular calcification. Here, we characterized MGP expression in cultured renal tubular epithelial cells exposed to oxalate and calcium oxalate monohydrate (COM) crystals.
  
  
  METHODS
  NRK-52E cells were cultured and exposed to medium containing oxalate or COM for 1, 3, 6, 12 and 24 h. The levels of MGP mRNA expression were quantified using real-time PCR (RT-PCR), and the levels of protein expression were characterized by Western blot.
  
  
  RESULTS
  MGP was expressed rapidly and intensively following exposure to COM and was time-dependent with exposure to oxalate.
  
  
  CONCLUSIONS
  Our results have given a profile of MGP expression in renal tubular epithelial cells exposed to oxalate and crystals and provided support for an association between MGP and kidney stones. Further investigations on the mechanism of the MGP in the process of crystal-cell interaction may be important in the future.
authors.affiliation: Department of Cell Biology and Genetics, Shenyang Medical College, Shenyang, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Gao
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yasui
authors.forename: Xiuli
authors.initials: X
authors.lastname: Lu
authors.forename: Hongge
authors.initials: H
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okada
authors.forename: Chunling
authors.initials: C
authors.lastname: Xiao
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Kohri
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002129:Calcium Oxalate / Q000737:chemistry / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003460:Crystallization; D004847:Epithelial Cells / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D019815:Oxalic Acid / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation; D000097568:Matrix Gla Protein
chemical_list: D002129:Calcium Oxalate; D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; D019815:Oxalic Acid; D012333:RNA, Messenger; D000097568:Matrix Gla Protein