[Fingerprint analysis of Dipsacus asperoides by HPLC].
OBJECTIVE
To establish the HPLC fingerprints of Dipsacus asperoides for reflecting the internal information, evaluating its internal quality.


METHODS
20 batches of D. asperoides were collected from different place with the HPLC fingerprints method. Chromatographic column: Welchrom-C18 (250 mm x 4. 6 mm, 5 microm), mobile phase: acetonitrile and water (gradient elution), flow rate: 1.0 mL/min, detection wavelength: 212 nm, column temperature: 35 degrees C.


RESULTS
The common mode of HPLC fingerprint was established and similar degrees to D. asperoides from different areas were compared.


CONCLUSIONS
The method is stable, reliable, and with full information which can be used for quality evaluation, quality control item and crude drug identification of D. asperoides.
Liu
Yong
Y
Chengdu University of TCM, Chengdu 611137, China. lys372@163.com
Wei
Ying-Fang
YF
Zhu
Jun-Ping
JP
Long
Fei
F
Guo
Shan-Shan
SS
Yan
Jie
J
Wang
Hua-Dong
HD
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D049917:Dipsacaceae / Q000737:chemistry* / Q000254:growth & development; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010598:Pharmacognosy; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D012503:Saponins