title: An in-tube SPME device for the selective determination of chlorophyll a in aquatic systems.
abstract: We report a new device for the estimation of the content of chlorophyll a pigment in water samples as an indicator of water quality. The extraction of the pigment from water was also optimized. 10 mL of water was filtered through a nylon filter (45 microm pore size and 13 mm of diameter), after the chlorophylls were dissolved by immersing the filter in 1 mL of a low non-hazardous solvent as ethanol. An in-valve in-tube SPME device coupled to capillary liquid chromatography with diode array detection was designed. A capillary column of 70 cm in length (0.32 mm i.d. coated with 5% diphenyl-95% polydimethylsiloxane, 3 microm coating thickness) was used as the loop of the injection valve for preconcentration and a Zorbax SB C(18) (SiO(2)-based) 150 mm x 0.5 mm i.d., 5 microm column (Agilent) was used as analytical column. The achieved detection limit was 0.05 microg L(-1) and the working range of concentrations was 0.1-1 microg L(-1). % RSD values between 2 and 11 were obtained. Chlorophyll a in several water matrices was determined with good results in presence of other pigments such as chlorophyll b, pheophytin a and pheophytin b.
authors.affiliation: School of Chemistry, Faculty of Science, Central University of Venezuela Paseo los Ilustres, Urb. Valle Abajo 20513, Carracas, Venezuela.
authors.forename: Y
authors.initials: Y
authors.lastname: Vitta
authors.forename: Y
authors.initials: Y
authors.lastname: Moliner-Martínez
authors.forename: P
authors.initials: P
authors.lastname: Campíns-Falcó
authors.forename: A Fernández
authors.initials: AF
authors.lastname: Cuervo
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D005374:Filtration; D057230:Limit of Detection; D010674:Pheophytins; D011786:Quality Control; D052616:Solid Phase Extraction / Q000295:instrumentation*; D012997:Solvents; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D010674:Pheophytins; D012997:Solvents; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; C037184:chlorophyll b; C061694:pheophytin a