Transgenic RNAi applications in the mouse.
Within the past 10 years, RNA interference has emerged as a powerful experimental tool as it allows rapid gene function analysis. Unique features such as reversibility of gene silencing and simultaneous targeting of several genes characterize the approach. In this chapter, transgenic RNAi techniques in reverse mouse genetics are discussed and protocols are provided.
Seibler
Jost
J
TaconicArtemis GmbH, Cologne, Germany.
Schwenk
Frieder
F
D000818:Animals; D020868:Gene Silencing / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D034622:RNA Interference / Q000502:physiology*