The peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica.
In this paper, we describe the CTA1 gene, which encodes a peroxisomal catalase in the methylotrophic yeast Pichia methanolica. The P. methanolica CTA1 gene (PmCTA1) comprises a 1,530-bp open reading frame corresponding to a protein of 510 amino acid residues, and its deduced amino acid sequence shows high similarity to those of Cta1ps from other methylotrophic yeasts (about 79%). Expression of PmCTA1 in a peroxisomal catalase-depleted (Cbcta1Delta) Candida boidinii strain restored the methylotrophic growth of the host strain, while the expression of PmCTA1-DeltaSRL, which lacks peroxisome targeting signal type 1, did not. In P. methanolica, expression of PmCTA1 was induced when cells were grown on peroxisome-inducing carbon sources, viz., methanol, oleate, and D-alanine. Taken together, these results indicate that PmCTA1 encodes a functional peroxisomal catalase in P. methanolica.
Nakagawa
Tomoyuki
T
Faculty of Applied Biological Science, Gifu University, Yanagido, Gifu, Japan. t-nakaga@gifu-u.ac.jp
Yoshida
Kyoko
K
Takeuchi
Akihito
A
Ito
Takashi
T
Fujimura
Shuki
S
Matsufuji
Yoshimi
Y
Tomizuka
Noboru
N
Yurimoto
Hiroya
H
Sakai
Yasuyoshi
Y
Hayakawa
Takashi
T
D000595:Amino Acid Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000201:enzymology*; D010843:Pichia / Q000201:enzymology* / Q000235:genetics*
D002374:Catalase