The structural biology of PRRSV.
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped, positive-sense single-stranded RNA virus belonging to the Arteriviridae family. Arteriviruses and coronaviruses are grouped together in the order Nidovirales, based on similarities in genome organization and expression strategy. Over the past decade, crystal structures of several viral proteins, electron microscopic studies of the virion, as well as biochemical and in vivo studies on protein-protein interactions have led to a greatly increased understanding of PRRSV structural biology. At this point, crystal structures are available for the viral proteases NSP1α, NSP1β and NSP4 and the nucleocapsid protein, N. The NSP1α and NSP1β structures have revealed additional non-protease domains that may be involved in modulation of host functions. The N protein forms a dimer with a novel fold so far only seen in PRRSV and other nidoviruses. Cryo-electron tomographic studies have shown the three-dimensional organization of the PRRSV virion and suggest that the viral nucleocapsid has an asymmetric, linear arrangement, rather than the isometric core previously described. Together, these studies have revealed a closer structural relationship between arteri- and coronaviruses than previously anticipated.
Dokland
Terje
T
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA. dokland@uab.edu
D018360:Crystallography, X-Ray; D008854:Microscopy, Electron; D008954:Models, Biological; D008958:Models, Molecular; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology* / Q000648:ultrastructure*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism; D014771:Virion / Q000648:ultrastructure
D014764:Viral Proteins