Serratia sp. ZF03: an efficient radium biosorbent isolated from hot-spring waters in high background radiation areas.
The aim of this study is to isolate and characterize (226)Ra biosorbing indigenous bacterial strains from soils and hot-springs containing high concentrations of (226)Ra by using biochemical and molecular approaches. Fifteen bacteria were isolated and their phylogenetic affiliations were determined based on their 16S rRNA gene and the two most relevant hypervariable regions of this gene; V3 and V6 analysis. A pigmented Serratia sp. ZF03 strain isolated from the water with (226)Ra content of 50471 mBq l(-1), caused 70% removal of (226)Ra at a radioactivity level of 50 Bq ml(-1), after 5 min and 75-80% in equilibrium time of 1 h, depending on the particular biosorption system and experimental conditions studied. The biosorption equilibrium was described by Langmuir and Freundlich isotherm models. Kinetic studies indicated that the biosorption follows pseudo-second-order kinetics. Effect of different physico-chemical parameters on (226)Ra sorption, FTIR, SEM and TEM analysis were also investigated.
Zakeri
Farideh
F
Department of Genetics, Faculty of Biological Science, Tarbiat Modares University, P.O. Box 14115-154, Tehran, Iran.
Noghabi
Kambiz Akbari
KA
Sadeghizadeh
Majid
M
Kardan
Mohammad Reza
MR
Masoomi
Fatemeh
F
Farshidpour
Mohammad Reza
MR
Atarilar
Ali
A
D000327:Adsorption; D001417:Background Radiation; D001673:Biodegradation, Environmental; D018533:Biomass; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007492:Iran; D007700:Kinetics; D010802:Phylogeny; D011883:Radium / Q000378:metabolism*; D012705:Serratia / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012990:Soil Pollutants, Radioactive / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014871:Water Microbiology; D014875:Water Pollutants, Radioactive / Q000032:analysis
D012990:Soil Pollutants, Radioactive; D014875:Water Pollutants, Radioactive; D011883:Radium