title: Osmoregulated trehalose-derived oligosaccharides in Sinorhizobium meliloti.
abstract: Sinorhizobium meliloti is a soil bacterium accumulating glutamate, N-acetylglutaminyl glutamine amide and trehalose in hyperosmolarity. Besides these compatible solutes, we highlighted several compounds in S. meliloti Rm1021 wild-type strain. The purification and the structural characterization based on liquid chromatography evaporative light scattering detector, electrospray ionization high resolution mass spectrometry and nuclear magnetic resonance techniques showed they were four linear oligosaccharides composed of 3, 4, 5 and 6 glucose units all linked by alpha-(1-->2) linkages except a terminal alpha-(1<-->1) linkage. These oligosaccharides were cytoplasmic and were observed in several wild-type strains suggesting they were common features in S. meliloti strains grown in hyperosmolarity.
authors.affiliation: Unité Génie Enzymatique et Cellulaire, UMR CNRS 6022, Université de Picardie Jules Verne, Amiens, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Brique
authors.forename: Jimi
authors.initials: J
authors.lastname: Devassine
authors.forename: Serge
authors.initials: S
authors.lastname: Pilard
authors.forename: Dominique
authors.initials: D
authors.lastname: Cailleu
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gosselin
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D016962:Sinorhizobium meliloti / Q000737:chemistry* / Q000378:metabolism; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014199:Trehalose / Q000737:chemistry*; D014882:Water-Electrolyte Balance
chemical_list: D009844:Oligosaccharides; D014199:Trehalose