title: Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research.
abstract: High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) is regarded as one of the most powerful techniques for separation and identification of proteins. Recently, much effort has been made to improve the separation capacity, detection sensitivity, and analysis throughput of micro- and nano-HPLC, by increasing column length, reducing column internal diameter, and using integrated techniques. Development of HPLC columns has also been rapid, as a result of the use of submicrometer packing materials and monolithic columns. All these innovations result in clearly improved performance of micro- and nano-HPLC for proteome research.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Dingyin
authors.initials: D
authors.lastname: Tao
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yichu
authors.initials: Y
authors.lastname: Shan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D006801:Humans; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011506:Proteins; D020543:Proteome