[Effect of qinghuobaiduyin on the expression of high mobility group box chromosomal protein 1 in macrophage].
OBJECTIVE
To observe the expression of high mobility group box chromosomal protein 1(HMGB1) in RAW264.7 macrophages after interfering with burning serum and qinghuobaidu-yin (QHBDY), and to find out the endogenous protection mechanism of QHBDY resisting inflammation reaction.


METHODS
RT-PCR was used to detect the expression of HMGB1 in RAW264.7 macrophages after interfering RAW264.7 macrophages with normal SD rat serum, burning SD rat serum, and QHBDY feeding SD rat serum.


RESULTS
Small quantity of HMGB1 mRNA was expressed in RAW264.7. The expression of HMGB1 mRNA fluctuated around the standard level after interfering with normal serum of SD rats. The expression of HMGB1 mRNA rose at 3 h, and then decreased to the standard level; at 18 h, it rose rapidly; at 36 h, it reached the peak; and at 48 h, it remained at the high level after interfering with burning serum. The expression of HMGB1 mRNA increased at 3 h, and then decreased to the standard level. At 24 h, it started to rise after interfering with herb serum, and was lower than that of; the burning serum group (P<0.05).


CONCLUSIONS
Burning serum can increase the expression of HMGB1 mRNA in RAW264.7. QHBDY can decrease the high expression of HMGB1 mRNA in RAW264.7 caused by burning serum.
Li
Ping
P
Department of Burn and Plastics Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China. lipin1973@yahoo.com.cn
Xu
Dan
D
Luo
Chengqun
C
D000818:Animals; D002056:Burns / Q000150:complications / Q000276:immunology*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018746:Systemic Inflammatory Response Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control
D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; D012333:RNA, Messenger