Cloning and characterization of phenylalanine ammonia-lyase and cinnamate 4-hydroxylase and pyranocoumarin biosynthesis in Angelica gigas.
Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) are important enzymes in the phenylpropanoid pathway and also in the accumulation of decursin (1) and decursinol angelate (2), which are major secondary metabolites in Angelica gigas. Using PCR with degenerate primers targeted to conserved regions of available orthologous PAL and C4H sequences, cDNAs encoding PAL and C4H from A. gigas were isolated. Both genes were used to show the comparative developmental and inducible accumulation of mRNAs in different organs and in suspension cells of A. gigas. PAL and C4H were induced most strongly in response to 300 microM methyl jasmonate treatment at 6 and 12 h, respectively, and were highly expressed in the fine roots of A. gigas. Similarly, the production of 1 and 2 was most prolific in the fine roots of the plant.
Park
Jee Hee
JH
Department of Crop Science, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-754, Republic of Korea.
Park
Nam Il
NI
Xu
Hui
H
Park
Sang Un
SU
D029969:Angelica / Q000201:enzymology / Q000235:genetics; D001578:Benzopyrans / Q000032:analysis / Q000737:chemistry; D002087:Butyrates / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant; D015394:Molecular Structure; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000378:metabolism*; D010666:Phenylpropionates / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D039681:Pyranocoumarins / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D050564:Trans-Cinnamate 4-Monooxygenase / Q000235:genetics* / Q000378:metabolism*
D001578:Benzopyrans; D002087:Butyrates; D010666:Phenylpropionates; D039681:Pyranocoumarins; D012333:RNA, Messenger; C101278:decursin; D050564:Trans-Cinnamate 4-Monooxygenase; D010650:Phenylalanine Ammonia-Lyase