title: Investigation on the Interactions of NiCR and NiCR-2H with DNA.
abstract: We report here a biophysical and biochemical approach to determine the differences in interactions of NiCR and NiCR-2H with DNA. Our goal is to determine whether such interactions are responsible for the recently observed differences in their cytotoxicity toward MCF-7 cancer cells. Viscosity measurement and fluorescence displacement titration indicated that both NiCR and NiCR-2H bind weakly to duplex DNA in the grooves. The coordination of NiCR-2H with the N-7 of 2'-deoxyguanosine 5'-monophosphate (5'-dGMP) is stronger than that of NiCR as determined by (1)H NMR. NiCR-2H, like NiCR, can selectively oxidize guanines present in distinctive DNA structures (e.g., bulges), and notably, NiCR-2H oxidizes guanines more efficiently than NiCR. In addition, UV and (1)H NMR studies revealed that NiCR is oxidized into NiCR-2H in the presence of KHSO(5) at low molar ratios with respect to NiCR (</=4).
authors.affiliation: Department of Chemistry, University of the Pacific, Stockton, CA 95211, USA.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Chitranshi
authors.forename: Chang-Nan
authors.initials: CN
authors.lastname: Chen
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Jones
authors.forename: Jesika S
authors.initials: JS
authors.lastname: Faridi
authors.forename: Liang
authors.initials: L
authors.lastname: Xue