title: Specific MALDI-MSI: Tag-Mass.
abstract: MALDI imaging as a molecular mass spectrometry imaging technique (MSI) can provide accurate information about molecular composition on a surface. The last decade of MSI development has brought the technology to clinical and biomedical applications as a complementary technique of MRI and other molecular imaging. Then, this IMS technique is used for endogenous and exogenous molecule detection in pharmaceutical and biomedical fields. However, some limitations still exist due to physical and chemical aspects, and sensitivity of certain compounds is very low. Thus, we developed a multiplex technique for fast detection of different compound natures. The multiplex MALDI imaging technique uses a photocleavable group that can be detect easily by MALDI instrument. These techniques of targeted imaging using Tag-Mass molecules allow the multiplex detection of compounds like antibodies or oligonucleotides. Here, we describe how we used this technique to detect huge proteins and mRNA by MALDI imaging in rat brain and in a model for regeneration; the leech.
authors.affiliation: Laboratoire de Neuroimmunologie et Neurochimie Evolutives, FRE CNRS 3249, MALDI Imaging Team, Université Lille Nord de France, Université Lille 1, Villeneuve d'Ascq, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Stauber
authors.forename: Mohamed El
authors.initials: ME
authors.lastname: Ayed
authors.forename: Maxence
authors.initials: M
authors.lastname: Wisztorski
authors.forename: Michel
authors.initials: M
authors.lastname: Salzet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fournier
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D007865:Leeches; D008962:Models, Theoretical; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D012333:RNA, Messenger