Residual tumor cells are unique cellular targets in glioblastoma.
Residual tumor cells remain beyond the margins of every glioblastoma (GBM) resection. Their resistance to postsurgical therapy is considered a major driving force of mortality, but their biology remains largely uncharacterized. In this study, residual tumor cells were derived via experimental biopsy of the resection margin after standard neurosurgery for direct comparison with samples from the routinely resected tumor tissue. In vitro analysis of proliferation, invasion, stem cell qualities, GBM-typical antigens, genotypes, and in vitro drug and irradiation challenge studies revealed these cells as unique entities. Our findings suggest a need for characterization of residual tumor cells to optimize diagnosis and treatment of GBM.
Glas
Martin
M
Institute of Reconstructive Neurobiology, University of Bonn Medical Center, Germany. martin.glas@ukb.uni-bonn.de
Rath
Barbara H
BH
Simon
Matthias
M
Reinartz
Roman
R
Schramme
Anja
A
Trageser
Daniel
D
Eisenreich
Ramona
R
Leinhaas
Anke
A
Keller
Mihaela
M
Schildhaus
Hans-Ulrich
HU
Garbe
Stephan
S
Steinfarz
Barbara
B
Pietsch
Torsten
T
Steindler
Dennis A
DA
Schramm
Johannes
J
Herrlinger
Ulrich
U
Brüstle
Oliver
O
Scheffler
Björn
B
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002469:Cell Separation; D005260:Female; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D009364:Neoplasm Recurrence, Local / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm