title: Identification of new pathogens in the intraocular fluid of patients with uveitis.
abstract: OBJECTIVE
  To determine infectious causes in patients with uveitis of unknown origin by intraocular fluids analysis.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Ocular fluids from 139 patients suspected of infectious uveitis, but negative for herpes simplex virus, varicella-zoster virus, cytomegalovirus, and Toxoplasma gondii by polymerase chain reaction and/or antibody analysis in intraocular fluids, were assessed for the presence of 18 viruses and 3 bacteria by real-time polymerase chain reaction (PCR). The ocular fluids from 48 patients with uveitis of known etiology or with cataract were included as controls.
  
  
  RESULTS
  Positive PCR results were found for Epstein-Barr virus, for rubella virus, and for human herpesvirus 6 each in 1 patient and for human parechovirus in 4 patients. Of the human parechovirus-positive patients, 1 was immunocompromised and had panuveitis. The other 3 patients were immunocompetent and had anterior uveitis, all with corneal involvement.
  
  
  CONCLUSIONS
  Human parechovirus might be associated with infectious (kerato)uveitis.
authors.affiliation: Department of Virology, University Medical Center Utrecht, The Netherlands. J.D.FdeGroot@umcutrecht.nl
authors.forename: Jolanda D F
authors.initials: JD
authors.lastname: de Groot-Mijnes
authors.forename: Lenneke
authors.initials: L
authors.lastname: de Visser
authors.forename: Stephanie
authors.initials: S
authors.lastname: Zuurveen
authors.forename: Roaldy A
authors.initials: RA
authors.lastname: Martinus
authors.forename: René
authors.initials: R
authors.lastname: Völker
authors.forename: Ninette H
authors.initials: NH
authors.lastname: ten Dam-van Loon
authors.forename: Joke H
authors.initials: JH
authors.lastname: de Boer
authors.forename: Gina
authors.initials: G
authors.lastname: Postma
authors.forename: Raoul J
authors.initials: RJ
authors.lastname: de Groot
authors.forename: Anton M
authors.initials: AM
authors.lastname: van Loon
authors.forename: Aniki
authors.initials: A
authors.lastname: Rothova
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001082:Aqueous Humor / Q000821:virology*; D016022:Case-Control Studies; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000821:virology; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000821:virology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D030061:Parechovirus / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000821:virology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D019349:Roseolovirus Infections / Q000175:diagnosis / Q000821:virology; D012409:Rubella / Q000175:diagnosis / Q000821:virology; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*; D014606:Uveitis, Anterior / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral