title: Analysis of 11 tetrameric STRs in wild boars for forensic purposes.
abstract: STR profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety. We tested the efficacy of a multiplex PCR-based assay including 11 porcine-specific tetrameric STRs in a population sample of wild boars (n=142) originating from Piedmont (North West Italy). Multiple deviations from Hardy-Weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative of a high degree of inbreeding. A value of θ of 0.046 and an inbreeding coefficient of 0.089 were estimated. Combined power of discrimination and probability of exclusion values for the STR panel were 0.9999999999996 and 0.99989. In order to test the suitability of the method for meat traceability purposes, a domestic pig reference sample (n=412), consisting of commercial lines commonly used in the meat production process, was also typed. A Bayesian cluster analysis carried out using the observed genotypes, showed a percentage of correct subspecies assignment of individual samples of 0.974 for wild boars and 0.991 for pigs, thus demonstrating the usefulness of the multiplex STR-typing system for discrimination purposes.
authors.affiliation: Department of Anatomy, Pharmacology and Legal Medicine, University of Turin, Corso Galileo Galilei 22, 10126 Turin, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Caratti
authors.forename: Luca
authors.initials: L
authors.lastname: Rossi
authors.forename: Bruno
authors.initials: B
authors.lastname: Sona
authors.forename: Silvia
authors.initials: S
authors.lastname: Origlia
authors.forename: Silvana
authors.initials: S
authors.lastname: Viara
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Martano
authors.forename: Carlo
authors.initials: C
authors.lastname: Torre
authors.forename: Carlo
authors.initials: C
authors.lastname: Robino
mesh_terms: D000818:Animals; D053803:Forensic Genetics; D006579:Heterozygote; D007558:Italy; D018895:Microsatellite Repeats; D034421:Sus scrofa / Q000235:genetics*