Binding of bisphenol A and acrylamide to BSA and DNA: insights into the comparative interactions of harmful chemicals with functional biomacromolecules.
The interactions between bisphenol A (BPA)/acrylamide (AA) and bovine serum albumin (BSA)/deoxyribonucleic acid (DNA) was investigated by the equilibrium dialysis, fluorophotometry, isothermal titration calorimetry (ITC) and circular dichroism (CD). The bindings of BPA and AA to BSA and DNA responded to the partition law and Langmuir isothermal model, respectively. The saturation mole number of AA was calculated to be 24 per mol BSA and 0.26 per mol DNA-P. All the reactions were spontaneous driven by entropy change. BPA stacked into the aromatic hydrocarbon groups of BSA and between adjacent basepairs of DNA via the hydrophobic effect. The interactions of AA with BSA and DNA induced the formation of hydrogen bond and caused changes of their secondary structures. At normal physiological condition, 0.100 mmol/l BPA reduced the binding of vitamin B(2) to BSA by more than 70%, and 2.8 mmol/l AA by almost one half. This work provides an insight into non-covalent intermolecular interaction between organic contaminant and biomolecule, helping to elucidate the toxic mechanism of harmful chemicals.
Zhang
Ya-Lei
YL
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Zhang
Xian
X
Fei
Xun-Chang
XC
Wang
Shi-Long
SL
Gao
Hong-Wen
HW
D020106:Acrylamide / Q000378:metabolism*; D001559:Benzhydryl Compounds; D004247:DNA / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013696:Temperature; D000098826:Bisphenol A Compounds
D020106:Acrylamide; D001559:Benzhydryl Compounds; D004247:DNA; D010636:Phenols; D012710:Serum Albumin, Bovine; C006780:bisphenol A; D000098826:Bisphenol A Compounds