title: Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization.
abstract: Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.
authors.affiliation: Institute of Plant Science and Resources, Okayama University 2-20-1, Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Fusako
authors.initials: F
authors.lastname: Kawai
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Kimbara
mesh_terms: D018407:Bacterial Physiological Phenomena; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D016958:Pseudomonas putida / Q000502:physiology