A novel Pzg-NURF complex regulates Notch target gene activity.
Drosophila putzig was identified as a member of the TRF2-DREF complex that is involved in core promoter selection. Additionally, putzig regulates Notch signaling, however independently of DREF. Here, we show that Putzig associates with the NURF complex. Loss of any NURF component including the NURF-specific subunit Nurf 301 impedes binding of Putzig to Notch target genes, suggesting that NURF recruits Putzig to these sites. Accordingly, Putzig can be copurified with any NURF member. Moreover, Nurf 301 mutants show reduced Notch target gene activity and enhance Notch mutant phenotypes. These data suggest a novel Putzig-NURF chromatin complex required for epigenetic activation of Notch targets.
Kugler
Sabrina J
SJ
Institute of Genetics, University of Hohenheim, 70599 Stuttgart, Germany.
Nagel
Anja C
AC
D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D002999:Clone Cells; D004247:DNA / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D051880:Receptors, Notch; C524392:pzg protein, Drosophila; D004247:DNA