title: Plasma free amino acid kinetics in rainbow trout (Oncorhynchus mykiss) using a bolus injection of 15N-labeled amino acids.
abstract: To gain insight into the metabolic design of the amino acid carrier systems in fish, we injected a bolus of (15)N amino acids into the dorsal aorta in mature rainbow trout (Oncorhynchus mykiss). The plasma kinetic parameters including concentration, pool size, rate of disappearance (R(d)), half-life and turnover rate were determined for 15 amino acids. When corrected for metabolic rate, the R(d) values obtained for trout for most amino acids were largely comparable to human values, with the exception of glutamine (which was lower) and threonine (which was higher). R(d) values ranged from 0.9 μmol 100 g(-1) h(-1) (lysine) to 22.1 μmol 100 g(-1) h(-1) (threonine) with most values falling between 2 and 6 μmol 100 g(-1) h(-1). There was a significant correlation between R(d) and the molar proportion of amino acids in rainbow trout whole body protein hydrolysate. Other kinetic parameters did not correlate significantly with whole body amino acid composition. This indicates that an important design feature of the plasma-free amino acids system involves proportional delivery of amino acids to tissues for protein synthesis.
authors.affiliation: Department of Integrative Biology, University of Guelph, 50 Stone Road East, RM. 3407 New Science Complex, Guelph, ON, N1G2W1, Canada. jrobin06@uoguelph.ca
authors.forename: Jacob William
authors.initials: JW
authors.lastname: Robinson
authors.forename: Dan
authors.initials: D
authors.lastname: Yanke
authors.forename: Jeff
authors.initials: J
authors.lastname: Mirza
authors.forename: James Stuart
authors.initials: JS
authors.lastname: Ballantyne
mesh_terms: D000596:Amino Acids / Q000097:blood* / Q000737:chemistry*; D000818:Animals; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D009587:Nitrogen Isotopes / Q000097:blood / Q000737:chemistry*; D017686:Oncorhynchus mykiss / Q000097:blood*
chemical_list: D000596:Amino Acids; D009587:Nitrogen Isotopes