Surface bacteriology of venous leg ulcers and healing outcome.
OBJECTIVE
Bacteria can be cultured from all venous leg ulcers (VLUs) regardless of healing status, and the significance of a positive swab result in non-clinically infected ulcers is unknown. The aim of this study was to characterise the bacteriological flora of VLUs by routine culture to determine whether the data generated had prognostic value.


METHODS
The ulcers of 178 patients were sampled weekly for 12 weeks and healing outcome monitored while the limb was treated with graduated compression. Wound bacteriology was assessed using culture methodology standardised to ensure data reproducibility.


RESULTS
153 individual bacterial species were identified. The species most frequently found were Staphylococcus aureus (64.3% of assessments), Corynebacterium striatum (60.6%), Pseudomonas aeruginosa (32.6%), Helcococcus kunzii (22.0%), Finegoldia magna (21.4%) and Proteus mirabilis (16.1%). No single species or the presence of anaerobes and increasing diversity of bacterial species, previously thought to be predictive of impaired healing, was shown to be associated with healing outcome. The presence of C striatum was associated with healing outcome but not after adjusting for the known prognostic factors of wound area and duration.


CONCLUSIONS
Routine bacteriological culture analysis of the VLU wound surface may be used to identify diverse flora in all ulcers. However, the data generated are of no additional value as a prognostic indicator of healing outcome. The presence of C striatum may represent colonisation of non-healing VLU by normal skin flora.
Moore
Keith
K
Department of Wound Healing, Cardiff University, School of Medicine, Cardiff, UK. keith@xyres.co.uk
Hall
Val
V
Paull
Alan
A
Morris
Trefor
T
Brown
Sarah
S
McCulloch
Dorothy
D
Richardson
Mark C
MC
Harding
Keith G
KG
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification* / Q000472:pathogenicity; D058128:Compression Bandages; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012867:Skin / Q000382:microbiology; D016896:Treatment Outcome; D014647:Varicose Ulcer / Q000382:microbiology* / Q000628:therapy; D014945:Wound Healing