title: Evaluation of the in vivo anti-inflammatory effects of extracts from Pyrus bretschneideri Rehd.
abstract: Pyrus bretschneideri Rehd., as a pharmaceutical supplement, is widely used in northern China to treat respiratory diseases. Our previous studies showed the ethanol extract of P. bretschneideri had significant anti-inflammatory activity. To isolate and identify the active ingredients, the ethanol extract was separated into petroleum ether, ethyl acetate, n-butanol, and aqueous fractions. The bioactivity of each fraction was investigated using an in vivo model. Results showed that the ethyl acetate fraction exhibited the strongest anti-inflammatory effect. Subsequently, this fraction was subjected to separation and purification using silica gel column chromatography, C18-ODS, and recrystallization, leading to two sterols and two triterpenes, which were identified as β-sitosterol, daucosterol, oleanolic acid, and ursolic acid. Moreover, all of the isolated compounds could significantly inhibit the ear edema induced by xylene. These results indicated that P. bretschneideri had good anti-inflammatory effects and the constituents β-sitosterol, daucosterol, oleanolic acid, and ursolic acid might well account for it.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Huang
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Wan-Shun
authors.initials: WS
authors.lastname: Zhao
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Chang-Xiao
authors.initials: CX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002850:Chromatography, Gel; D004353:Drug Evaluation, Preclinical; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D031989:Pyrus / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts