Potential c-myc antisense oligonucleotide carriers: PCl/PEG/PEI and PLL/PEG/PEI.
In this work, positively charged, micelle-forming polymers were synthesized and used as a model vector to deliver antisense oligodeoxynucleotide (ASODN) into melanoma cells. Polymers and polymer/ASODN complexes were characterized by DLS according to size, charge, and critical micelle concentration. Nanosize and spherical complexes were observed by AFM. Complexes did not reveal significant toxicity to melanoma cells. Antiproliferative effect of the complexes was observed by immunocytochemical staining and estimated as 56.8% with N/P:9. High amount of apoptosis and very small amount of necrosis were estimated. According to the results, these positively charged polymers forming micelle-like structures seem promising as ASODN carriers.
Dinçer
Sevil
S
Yıldız Technical University, Bioengineering Department, Davutpasa, Istanbul, Turkey. sevild@yildiz.edu.tr
Türk
Mustafa
M
Karagöz
Ayşe
A
Uzunalan
Gürhan
G
D017209:Apoptosis / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008823:Micelles; D036103:Nanotechnology; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000235:genetics; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use*
D008823:Micelles; D016376:Oligonucleotides, Antisense; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D011092:Polyethylene Glycols; C033616:poly(lactide); D011094:Polyethyleneimine