Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells.
Despite recent cell-engineering advances, treatment and repair of cartilage remains challenging. Although stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a prominent strategy, the major problem of limited proliferative capacity of autologous cells has been unsolved. Recently, an induced pluripotent stem (iPS) cell line was suggested as an alternative way to cure various human diseases due to their potential proliferating infinitely while possessing the capacity to form all types of cells. However, the method to induce lineage-restricted differentiation has not been well examined or established. Here, we suggest a simple method to induce mesenchymal progenitors possessing chondrogenic property from mouse iPS cells. The MSC-like cells produced in our study expressed some MSC markers, and could also differentiate to osteoblast and adipocyte. The present study demonstrates the property of iPS cells as an alternative candidate for treatment of articular disorders, and suggests an effective approach for preparing chondrocyte from iPS cells.
Teramura
Takeshi
T
Institute of Advanced Clinical Medicine, Kinki University School of Medicine, Osaka, Japan. teramura@med.kindai.ac.jp
Onodera
Yuta
Y
Mihara
Toshihiro
T
Hosoi
Yoshihiko
Y
Hamanishi
Chiaki
C
Fukuda
Kanji
K
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology*; D017931:DNA Primers; D005434:Flow Cytometry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*
D017931:DNA Primers