CD133+ stem cell mobilization after partial hepatectomy depends on resection extent and underlying disease.
BACKGROUND
Bone marrow stem cells (BMSC) can participate to liver regeneration. However, conflicting results have been reported on this topic in patients undergoing liver resection.


OBJECTIVE
To assess the impact of liver resection extent and presence of underlying liver disease in modulating BMSC mobilization.


METHODS
We enrolled 29 patients undergoing liver resection of different extents, 5 surgical controls and 10 blood donors. Circulating CD133+ BMSC were measured by flow cytometry at different time-points after surgery. The hepatic commitment of mobilized BMSC was investigated by polymerase chain reaction. Liver specimens were collected during surgery for histopathological analysis. Hepatocyte growth factor and granulocyte-colony stimulating factor serum levels were measured by enzyme-linked immunosorbent assay.


RESULTS
BMSC mobilization was found in patients undergoing major liver resection, especially in the presence of underlying disease. Ductular reactions were noted in patients with chronic hepatopathy and the hepatic progenitor-like cells expressed CD133, NCAM, cytokeratin-19, and alpha-fetoprotein. Hepatocyte growth factor and granulocyte-colony stimulating factor levels increased following liver resection and the contemporaneous presence of liver disease was associated with their highest raise.


CONCLUSIONS
Liver repair is mainly an endogenous process. BMSC become important in case of extensive resection, especially in the presence of underlying hepatopathy and hepatic progenitor-like cells activation. Hepatocyte growth factor and granulocyte-colony stimulating factor seem to be involved in the dynamics underlying hepatic regeneration and BMSC recruitment.
Zocco
Maria Assunta
MA
Department of Internal Medicine, Catholic University of Rome, Italy.
Piscaglia
Anna Chiara
AC
Giuliante
Felice
F
Arena
Vincenzo
V
Novi
Marialuisa
M
Rinninella
Emanuele
E
Tortora
Annalisa
A
Rumi
Carlo
C
Nuzzo
Gennaro
G
Vecchio
Fabio Maria
FM
Bombardieri
Giuseppe
G
Gasbarrini
Antonio
A
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D019650:Hematopoietic Stem Cell Mobilization; D006498:Hepatectomy / Q000379:methods*; D017228:Hepatocyte Growth Factor / Q000097:blood; D006801:Humans; D053539:Keratin-19 / Q000097:blood; D008107:Liver Diseases / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D008875:Middle Aged; D019006:Neural Cell Adhesion Molecules / Q000097:blood; D010455:Peptides / Q000097:blood*; D000509:alpha-Fetoproteins / Q000378:metabolism
D000071916:AC133 Antigen; D015703:Antigens, CD; D015415:Biomarkers; D006023:Glycoproteins; D053539:Keratin-19; D019006:Neural Cell Adhesion Molecules; C000606023:PROM1 protein, human; D010455:Peptides; D000509:alpha-Fetoproteins; D016179:Granulocyte Colony-Stimulating Factor; D017228:Hepatocyte Growth Factor