title: A promoter polymorphism -2122C>T of CHI3L1 is associated with serum low density lipoprotein cholesterol level in Korean subjects.
abstract: OBJECTIVE
  This study assessed the effects of 10 tagging single nucleotide polymorphisms (SNPs) of the CHI3L1 gene on serum LDL cholesterol levels in 290 Korean subjects.
  
  
  METHODS
  Genotyping analyses of SNPs were conducted by TaqMan® method. The effects of the promoter SNP on mRNA expression and nuclear factor binding were measured by real-time PCR and electrophoretic mobility shift assay, respectively.
  
  
  RESULTS
  Among 10 tagging SNPs, -2122C>T SNP (rs946261) in the promoter region was significantly associated with serum LDL cholesterol level (P=0.005). The T allele of -2122C>T was associated with significantly increased mRNA expressions in peripheral blood cells of the subjects, and also increased a nuclear factor binding measured by an electrophoretic mobility shift assay.
  
  
  CONCLUSIONS
  -2122C>T of CHI3L1, a promoter SNP which affects the mRNA expression and nuclear factor binding, is significantly associated with serum LDL cholesterol levels in Korean subjects.
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Haeyong
authors.initials: H
authors.lastname: Lee
authors.forename: Ryunhwa
authors.initials: R
authors.lastname: Kang
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Jee
authors.forename: Yoosik
authors.initials: Y
authors.lastname: Yoon
mesh_terms: D054392:Adipokines; D044466:Asian People / Q000235:genetics*; D020029:Base Pairing / Q000235:genetics; D000071451:Chitinase-3-Like Protein 1; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D055442:Metabolome / Q000235:genetics; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D056910:Republic of Korea
chemical_list: D054392:Adipokines; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D008078:Cholesterol, LDL; D006023:Glycoproteins; D037102:Lectins; D009687:Nuclear Proteins; D012333:RNA, Messenger