title: Crystallization of zinc finger proteins bound to DNA.
abstract: A method is presented for determining conditions for the cocrystallization of zinc finger proteins with DNA. The method describes steps beginning with protein expression, through purification, design of DNA for cocrystallization, and the conditions to screen for cocrystallization.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ, USA.
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Horton
authors.forename: Chad K
authors.initials: CK
authors.lastname: Park
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D004247:DNA; D000645:Ammonium Sulfate