Callus induction and plant regeneration in Alternanthera philoxeroides.
To establish a successful in vitro plant regeneration system in Alternanthera philoxeroides (Mart.) Griseb, an orthogonal design was used to investigate the effects of three factors (plant growth regulators, explant types and dark treatment in initial-stage), each having three levels. The effects of these factors and levels on callus induction and shoot regeneration were quantitatively evaluated by analysis of variance. The experimental results showed that the callus induction was significantly affected by 2, 4-dichlorophenoxyacetic acid (2, 4-D), and shoot differentiation from subcultured pieces of callus was enhanced mostly by dark treatment in initial-stage. The optimal conditions for callus induction are obtained from the stem explants cultured on semi-solid Murashige and Skoog (MS) medium plus 2.2 μM BA and 2.2 μM 2, 4-D, with 20 days dark treatment in initial-stage. The highest frequency of shoot regeneration is obtained from the calli cultured on semi-solid MS medium plus 8.8 μM BA, without dark treatment in initial-stage.
Gao
Jianming
J
China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International Cooperation base of Ministry of Science and Technology, College of Life Science and Technology, Huazhong University of Science & Technology, Luoyu Road 1037, Wuhan, Hubei, 430074, China.
Li
Jinzhi
J
Luo
Caibo
C
Yin
Liang
L
Li
Shengchun
S
Yang
Guangxiao
G
He
Guangyuan
G
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D027703:Amaranthaceae / Q000235:genetics* / Q000254:growth & development; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D008954:Models, Biological; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D018521:Plant Physiological Phenomena; D018520:Plant Shoots / Q000187:drug effects*; D012038:Regeneration / Q000187:drug effects*; D046509:Tissue Culture Techniques / Q000379:methods
D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid