title: Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium.
abstract: The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 degrees C and 37 degrees C, reaching a maximum production of 1.0 x 10(9) AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity.
authors.affiliation: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo-SP, Brazil.
authors.forename: Svetoslav Dimitrov
authors.initials: SD
authors.lastname: Todorov
authors.forename: Monica
authors.initials: M
authors.lastname: Wachsman
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Tomé
authors.forename: Xavier
authors.initials: X
authors.lastname: Dousset
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Destro
authors.forename: Leon Milner Theodore
authors.initials: LM
authors.lastname: Dicks
authors.forename: Bernadette Dora Gombossy de Melo
authors.initials: BD
authors.lastname: Franco
authors.forename: Manuella
authors.initials: M
authors.lastname: Vaz-Velho
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D000998:Antiviral Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D016984:Enterococcus faecium / Q000378:metabolism / Q000502:physiology*; D005516:Food Microbiology; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008087:Listeria / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D012474:Salmon / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D001430:Bacteriocins