title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2.
abstract: Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 A resolution and belonged to space group P2, with unit-cell parameters a=70.2, b=52.2, c=153.9 A, beta=102.8 degrees.
authors.affiliation: Key Laboratory of Environment Veterinary Science, Shanxi Agricultural University, Shanxi, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Cheng
authors.forename: Guangwen
authors.initials: G
authors.lastname: Lu
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yan
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017011:Streptococcus suis / Q000201:enzymology*
chemical_list: D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase