title: Characterization of emtricitabine related substances by liquid chromatography coupled to an ion trap mass spectrometer.
abstract: Emtricitabine (FTC) is an antiretroviral compound that inhibits the HIV-1 reverse transcriptase. For the quantification of FTC related substances, a liquid chromatography (LC) method coupled with ultraviolet (UV) detection was developed earlier in our laboratory. Several unknown compounds were detected during the analysis of a commercial sample. However, most of these impurities were not characterized. In this study, impurity profiling in a selected FTC sample was done using LC-mass spectrometry (MS). Due to the presence of a non-volatile buffer, a desalting procedure was carried out before sending the impurity into the MS. Totally, nine peaks were studied and most of them could be characterized.
authors.affiliation: Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Analyse, O&N2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Murali
authors.initials: M
authors.lastname: Pendela
authors.forename: Dunge Ashenafi
authors.initials: DA
authors.lastname: Mamade
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
authors.forename: Erwin
authors.initials: E
authors.lastname: Adams
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D000068679:Emtricitabine; D013058:Mass Spectrometry / Q000379:methods*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry*; D012492:Salts / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D018894:Reverse Transcriptase Inhibitors; D012492:Salts; D003841:Deoxycytidine; D000068679:Emtricitabine