Antithrombin Cambridge II(A384S) mutation frequency and antithrombin activity levels in 120 of deep venous thrombosis and 150 of cerebral infarction patients in a single center in Southern China.
Antithrombin Cambridge II(A384S) mutation shows a relatively high frequency in western population. Some studies suggest that the mutation is an independent genetic risk factor both for deep vein thrombosis (DVT) and for arterial thrombosis, but whether the mutation has racial difference or has a general significance for thrombophilia remains unclear. In this study we performed an analysis of the prevalence of the mutation in Chinese southern population; Also, the antithrombin activity levels were evaluated in each investigated individual. The studies included 120 patients with DVT, 150 patients with cerebral infarction, and 110 controls. The mutation was detected using polymerase chain reaction/PvuII restrictive fragment length polymorphism procedures. Antithrombin activity assay was done using chromogenic substrate method. The results showed that no antithrombin Cambridge II mutation was detected in all three groups (DVT, cerebral infarction and controls), the incidence was 0/380. Plasma antithrombin activity was 91.37% +/- 16.15% in the DVT patients and 102.68% +/- 13.10% in the controls; the antithrombin activity was significantly reduced in the DVT group (P < 0.0001). In DVT patients, eight cases were identified as primary antithrombin deficiency, accounting for an incidence of 6.7%. No significant difference was found for antithrombin activity between cerebral infarction group and controls. These results suggest that antithrombin Cambridge II mutation has a racial difference, and may not be a valuable risk factor of thrombophilia in Asian population, and antithrombin deficiency remains a major genetic risk factor for DVT patients in China.
Zhang
Guang-sen
GS
Department of Hematology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, P.R China. zgsllzy@163.com
Tang
Yang-ming
YM
Tang
Mei-qing
MQ
Qing
Zi-Ju
ZJ
Shu
Chang
C
Tang
Xiang-qi
XQ
Deng
Ming-yang
MY
Tan
Li-ming
LM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000990:Antithrombin III / Q000235:genetics / Q000378:metabolism*; D020152:Antithrombin III Deficiency / Q000150:complications*; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D002648:Child; D002681:China; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015995:Prevalence; D044469:Racial Groups; D020246:Venous Thrombosis / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D055815:Young Adult
C069998:antithrombin Cambridge II; D000990:Antithrombin III