title: Does cellular hydrogen peroxide diffuse or act locally?
abstract: Understanding of redox signaling requires data on the spatiotemporal distribution of hydrogen peroxide (H(2)O(2)) within the cell. The fluorescent reporter HyPer is a powerful instrument for H(2)O(2) imaging. However, rapid diffusion of HyPer throughout the nucleocytoplasmic compartment does not allow visualization of H(2)O(2) gradients on the micrometer scale. Here we dramatically improved the spatial resolution of H(2)O(2) imaging by applying subcytoplasmic targeting of HyPer. The membrane-attached reporters identified "microdomains" of elevated H(2)O(2) levels within the cytoplasm of the cells exposed to growth factors. We demonstrate that diffusion of H(2)O(2) across the cytoplasm was strongly limited, providing evidence that H(2)O(2) acts locally inside cells.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Mishina
authors.forename: Pyotr A
authors.initials: PA
authors.lastname: Tyurin-Kuzmin
authors.forename: Kseniya N
authors.initials: KN
authors.lastname: Markvicheva
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Vorotnikov
authors.forename: Vsevolod A
authors.initials: VA
authors.lastname: Tkachuk
authors.forename: Vibor
authors.initials: V
authors.lastname: Laketa
authors.forename: Carsten
authors.initials: C
authors.lastname: Schultz
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Vsevolod V
authors.initials: VV
authors.lastname: Belousov
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D004058:Diffusion; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D015398:Signal Transduction
chemical_list: D006861:Hydrogen Peroxide