title: Characterization and spatial distribution of bacterial communities within passively aerated cattle manure composting piles.
abstract: The bacterial communities in the core, bottom, top, middle-surface, and lower-surface full-scale passively aerated cattle manure compost was investigated using DGGE of PCR-amplified 16S rRNA sequences. Some Bacillus species and strictly anaerobic thermophilic Clostridium species were dominant only in the core and bottom zones. In contrast, bands belonging to mesophilic bacteria such as Bacteroidetes, Clostoridia,alpha and gamma-proteobacteria were detected in surface zones, even in the initial thermophilic stage of the process. Our results clearly show the spatial distribution of the microbial community within full-scale composting piles, which indicates N or C conversion by zone-specific bacterial communities were occurring in each zone of the pile.
authors.affiliation: Hokkaido Research Subteam for Waste Recycling System, National Agricultural Research Center for Hokkaido Region, National Agricultural and Food Research Organization, 1 Hitsujigaoka, Sapporo 062 8555, Japan. k_maeda@affrc.go.jp
authors.forename: Koki
authors.initials: K
authors.lastname: Maeda
authors.forename: Dai
authors.initials: D
authors.lastname: Hanajima
authors.forename: Riki
authors.initials: R
authors.lastname: Morioka
authors.forename: Takashi
authors.initials: T
authors.lastname: Osada
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000254:growth & development*; D002417:Cattle; D003013:Clostridium / Q000235:genetics / Q000254:growth & development*; D058645:Denaturing Gradient Gel Electrophoresis; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D013499:Surface Properties; D013696:Temperature
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil