title: Vitek 2 ANC card versus BBL Crystal Anaerobe and RapID ANA II for identification of clinical anaerobic bacteria.
abstract: The Vitek 2 Anaerobe and Corynebacterium Identification Card (ANC) was recently evaluated in a multicentre study. In the present work, this system was compared with the BBL Crystal Anaerobe and RapID ANA II panels. These kits were tested using 196 strains of anaerobes that had been previously identified by gas-liquid chromatography. Identification to the species or to the genus level was 75.0%, 81.1% and 70.9% for Crystal, RapID and Vitek, respectively. Vitek ANC failed to provide any identification in 20.4% of the strains, but it had fewer misidentifications than RapID. The confidence factors provided on the results report of each kit were not always correlated with a lower risk of major errors, with the exception of Vitek 2 in which a confidence factor higher than 0.86 excluded the risk of misidentification in more than 87% of isolates. The lower rate of identification by the Vitek and Crystal panels is mostly due the lower ability of these systems to identify the Clostridia. Overall, the three panels are comparable but need improvement to a better accuracy.
authors.affiliation: Department of Microbiology, St-Pierre University Hospital, Brussels, Belgium. laurent_blairon@stpierre-bru.be
authors.forename: Laurent
authors.initials: L
authors.lastname: Blairon
authors.forename: Mengi L
authors.initials: ML
authors.lastname: Maza
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wybo
authors.forename: Denis
authors.initials: D
authors.lastname: Piérard
authors.forename: Anne
authors.initials: A
authors.lastname: Dediste
authors.forename: Olivier
authors.initials: O
authors.lastname: Vandenberg
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity