title: Oroxylin A, a flavonoid, stimulates adult neurogenesis in the hippocampal dentate gyrus region of mice.
abstract: Previously, we reported the cognitive enhancing effects of oroxylin A in unimpaired mice and its memory ameliorating activity in various memory impaired mice. To elucidate the mechanism mediating the cognitive effects of oroxylin A, this study examined the consequences of oroxylin A administration on neurogenesis in the hippocampal dentate gyrus using immunostaining for 5-bromo-2-deoxyuridine (BrdU) incorporation. In addition, we determined whether the new cells adopted a neuronal or glial fate by examining the co-localization of BrdU staining with neuronal or glial markers. Administration of oroxylin A in a dose-dependent and time-dependent manner increased the number of BrdU-incorporating cells. Moreover, the percentage of BrdU-incorporating cells co-localized with neuronal markers, neuronal nuclei, was significantly increased by the oroxylin A administration. These results suggest that the increased neurogenesis induced by the administration of oroxylin A could be, at least in part, associated with its positive effects on cognitive processing.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Dongdaemoon-Ku, Seoul, Republic of Korea.
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Lee
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Lee
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jeon
authors.forename: Chang Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Ji Wook
authors.initials: JW
authors.lastname: Jung
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Shin
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D018891:Dentate Gyrus / Q000187:drug effects / Q000502:physiology*; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D055495:Neurogenesis / Q000187:drug effects*
chemical_list: D005419:Flavonoids; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one; D001973:Bromodeoxyuridine