[The effect of chimonin on collagen metabolism of pulmonary arterioles in chronic hypoxic rats].
OBJECTIVE
To study the effect and mechanism of chimonin on pulmonary arterioles I and III type collagen metabolism in pulmonary hypertension rats induced by chronic hypoxic hypercapnia.


METHODS
Thirty-six Sprague-Dawley rats were randomly divided into three groups: normal control group(A), hypoxic hypercapnic group(B), hypoxic hypercapnia + chimonin group(C). Collagen I, III and their mRNA, Blood CO concentration (COHb%), activity of HO-1 in blood serum and lung homogenate, content of hydroxyproline in lung homogenate, pulmonary arteriole micromorphometric index were observed.


RESULTS
Hypoxic hypercapnic rats's mPAP, Hyr of lung homogenate, content of I type collagen and I type collagen mRNA in pulmonary arterioles, were significantly higher than those in control group, pulmonary vessel remodeling of hypoxic hypercapnic rats was significant, those changes in hypercapnia + chimonin group were significantly lower than those in hypoxic hypercapnic group. Blood CO concentration, activity of HO-1 in blood serum and lung homogenate in rats of hypoxic hypercapnic rats were significantly higher than those of control group, and those of hypercapnia + chimonin group were even higher than hypoxic hypercapnic group (P < 0.01). There was no significant difference in mCAP, content of III type collagen and their mRNA in three groups (P > 0.05).


CONCLUSIONS
Chimonin can reduce pulmonary hypertension and pulmonary vessel remodeling induced by hypoxic hypercapnia through inhibiting proliferation of collagen I, the mechanism maybe is up regulating endogenous carbon monoxide system.
Huang
Xiao-ying
XY
Department of Respiration, The First Hospital of Wenzhou Medical College, Wenzhou 325003, China. zjwzhxy@126.com
Xu
Xiao-mei
XM
Qian
Guo-qing
GQ
Wang
Liang-xing
LX
D000818:Animals; D001160:Arterioles / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D002908:Chronic Disease; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006935:Hypercapnia / Q000150:complications / Q000503:physiopathology; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D000860:Hypoxia / Q000150:complications / Q000503:physiopathology*; D008168:Lung / Q000098:blood supply*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; C576566:chimonin; D002248:Carbon Monoxide