title: Effects of Roundup Transorb on fish: hematology, antioxidant defenses and acetylcholinesterase activity.
abstract: Roundup Transorb(RDT) is a glyphosate-based herbicide containing a mixture of surfactants. The objective of this work was to evaluate the effects of this herbicide on the Neotropical fish Prochilodus lineatus. Juvenile fish were acutely exposed (6, 24 and 96 h) to 1 mg L(-1) of RDT (RDT 1), 5 mg L(-1) of RDT (RDT 5) or only water (control) and blood samples for hematological analysis, liver for antioxidants analysis, and brain and muscle for acetylcholinesterase (AChE) determination, were collected. RDT effects were more evident in fish exposed to the higher concentration of the herbicide. Hematologic alterations appeared only after 96 h exposure, when fish showed an increase in the hematocrit and in the number of both red and white blood cells. After 6h exposure fish showed a transient reduction in superoxide dismutase and catalase activity. RDT also inhibited glutathione-S-transferase, after 6 and 24h of exposure. The reduction in these enzymes is probably related to the occurrence of lipid peroxidation (LPO) in fish exposed to the herbicide for 6h. LPO returned to control levels after 24 and 96 h exposure to RDT, when fish showed an increased activity of glutathione peroxidase. The content of reduced glutathione also increased after 96 h exposure. Thus, after 24 and 96 h the antioxidant defenses were apparently enough to combat ROS, preventing the occurrence of oxidative damage. The exposure to RDT for 96 h led to an inhibition of AChE in brain and muscle at rates which may not be considered a life-threatening situation.
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina, 86051-990, Londrina, Paraná C.P. 6001, Brazil.
authors.forename: Kathya A
authors.initials: KA
authors.lastname: Modesto
authors.forename: Cláudia B R
authors.initials: CB
authors.lastname: Martinez
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005399:Fishes / Q000097:blood / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006403:Hematologic Tests; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity; D000097797:Glyphosate
chemical_list: D000110:Acetylcholinesterase; D000975:Antioxidants; D002374:Catalase; D005982:Glutathione Transferase; D005998:Glycine; D006540:Herbicides; D013482:Superoxide Dismutase; D014874:Water Pollutants, Chemical; D000097797:Glyphosate