MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells.
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that inhibit gene expression post-transcriptionally. By regulating their target genes, miRNAs play important roles in tumor generation and development. Recently, the mir-200 family was revealed to inhibit the epithelial-mesenchymal transition, which is viewed as an essential step in early tumor metastasis. Here, we used luciferase assays to demonstrate that mir-200b interacts with predicted target sites in the 3' untranslated region of RND3. In HeLa cells, mir-200b directly reduced the expression of RND3 at the mRNA and protein levels, which thereby promoted expression of the downstream protein cyclin D1 and increased S-phase entry. In conclusion, our study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target.
Xia
Wei
W
Beijing Institute of Basic Medical Sciences, Beijing, China.
Li
Jie
J
Chen
Liucun
L
Huang
Baochun
B
Li
Shaohua
S
Yang
Guang
G
Ding
Hongmei
H
Wang
Fang
F
Liu
Nongle
N
Zhao
Qiang
Q
Fang
Tao
T
Song
Tao
T
Wang
Tianyou
T
Shao
Ningsheng
N
D001483:Base Sequence; D015153:Blotting, Western; D056741:Cyclin D / Q000235:genetics*; D017931:DNA Primers; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D020741:rho GTP-Binding Proteins / Q000502:physiology*
D056741:Cyclin D; D017931:DNA Primers; D035683:MicroRNAs; C508705:RND3 protein, human; D020741:rho GTP-Binding Proteins