Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety: Effect of elasticity of hydrogel.
We measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p-styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3-300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC-specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro. Real-time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC-specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC-specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffold, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels.
Yang
Jing Jing
JJ
Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
Chen
Yong Mei
YM
Kurokawa
Takayuki
T
Gong
Jian Ping
JP
Onodera
Shin
S
Yasuda
Kazunori
K
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D015870:Gene Expression; D019276:Glycocalyx / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D010973:Platelet Adhesiveness; D013451:Sulfonic Acids / Q000737:chemistry*; D013499:Surface Properties
D015415:Biomarkers; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D020100:Hydrogels; D013451:Sulfonic Acids; C071927:perlecan