title: Effects of microgravity modeled by large gradient high magnetic field on the osteogenic initiation of human mesenchymal stem cells.
abstract: Microgravity (MG) leads to a decrease in osteogenic potential of human bone marrow-derived mesenchymal stem cells (hMSCs). In the present study, we used large gradient high magnetic field (LGHMF) produced by a superconducting magnet to model MG (LGHMF-MG) and analyzed the effects of LGHMF-MG on survival, cytoskeleton and osteogenic potential of hMSCs. Results showed that the LGHMF-MG treatment for 6 h disrupted the cytoskeleton of hMSCs, and the LGHMF-MG treatment for 24 h led to cell death. LGHMF-MG treatments for 6 h in early stages of osteogenic induction (the pre-treatment before osteogenic induction, the beginning-treatment in the beginning-stage of osteogenic induction and the middle-treatment in the middle-stage of osteogenic induction) resulted in suppression on osteogenesis of hMSCs. The suppression intensity was reduced gradually as the treatment stage of LGHMF-MG was postponed. The LGHMF-MG treatment for 6 h in the ending-stage of osteogenic induction (the ending-treatment) had no obvious effect on osteogenesis of hMSCs. These results indicated that LGHMF-MG should affect the initiation of osteogenesis. Finally, the possible mechanism for the inhibition effect of LGHMF-MG on osteogenesis of hMSCs is discussed.
authors.affiliation: Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Room 307, Building of Life Sciences, Zijingang Campus, No.388, Yuhangtang Road, Hangzhou, Zhejiang, 310058, People's Republic of China.
authors.forename: Dongyan
authors.initials: D
authors.lastname: Shi
authors.forename: Rui
authors.initials: R
authors.lastname: Meng
authors.forename: Wanglong
authors.initials: W
authors.lastname: Deng
authors.forename: Wenchao
authors.initials: W
authors.lastname: Ding
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zheng
authors.forename: Wenji
authors.initials: W
authors.lastname: Yuan
authors.forename: Liyue
authors.initials: L
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Zong
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.forename: Jinfu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014893:Weightlessness
chemical_list: D000469:Alkaline Phosphatase