Synthesis of new 3-aryl-4,5-dihydropyrazole-1-carbothioamide derivatives. An investigation on their ability to inhibit monoamine oxidase.
Some differently substituted 3-aryl-4,5-dihydropyrazoles-1-carbothioamides have been synthesised with the aim to investigate their monoamine oxidase inhibitory activity. The chemical structures of the compounds have been characterized by means of their IR, (1)H NMR, (13)C NMR spectroscopic data and elemental analyses. All the active compounds showed a selective activity towards the B isoform of the enzyme, regardless of the substitution on the heterocyclic ring. The inhibition of the enzymatic activity was measured on human recombinant MAO isoforms, expressed in baculovirus infected BTI insect cells. Docking experiments were carried out with the aim to rationalize the mechanism of inhibition of the most active and selective compound.
Maccioni
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E
Dipartimento Farmaco Chimico Tecnologico, Via Ospedale 72 - 09124 Cagliari, Italy.
Alcaro
S
S
Orallo
F
F
Cardia
M C
MC
Distinto
S
S
Costa
G
G
Yanez
M
M
Sanna
M L
ML
Vigo
S
S
Meleddu
R
R
Secci
D
D
D000818:Animals; D002460:Cell Line; D006801:Humans; D007313:Insecta; D008958:Models, Molecular; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D011720:Pyrazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013329:Structure-Activity Relationship; D013854:Thioamides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*
D008996:Monoamine Oxidase Inhibitors; D011720:Pyrazoles; D011994:Recombinant Proteins; D013854:Thioamides; D008995:Monoamine Oxidase