title: An economical single-sided antibody incubation method for Western blotting.
abstract: A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Chuanying
authors.initials: C
authors.lastname: Pan
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Colin E
authors.initials: CE
authors.lastname: Bishop
mesh_terms: D000906:Antibodies / Q000032:analysis*; D015153:Blotting, Western / Q000191:economics* / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies