New coated SPME fibers for extraction and fast HPLC determination of selected drugs in human blood.
Polythiophene (PTh) and polypyrrole (PPy) as sorbent phases for solid phase microextraction (SPME) were applied in order to extract the multi-resistant Staphylococcus aureus (MRSA) antibiotic drugs (linezolid and daptomycin) from whole blood followed by high performance liquid chromatography (HPLC) determination with UV detection. Relative standard deviations (RSDs) of in vitro and pseudo in vivo measurements performed in whole blood were in the range of 4.58-15.91% and 6.09-17.33% for linezolid and daptomycin, respectively. Determination coefficients (R(2)) were in range of 0.9884-0.9945 and 0.9807-0.9818 for linezolid and daptomycin, respectively. This study proved better adsorption capacity of PTh SPME coating compared to PPy coating for both, linezolid and daptomycin.
Olszowy
Pawel
P
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, Torun, Poland.
Szultka
Malgorzata
M
Fuchs
Patricia
P
Kegler
Ricarda
R
Mundkowski
Ralf
R
Miekisch
Wolfram
W
Schubert
Jochen
J
Buszewski
Boguslaw
B
D000081:Acetamides / Q000097:blood*; D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017576:Daptomycin / Q000097:blood*; D006801:Humans; D000069349:Linezolid; D023303:Oxazolidinones / Q000097:blood*; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D013876:Thiophenes / Q000737:chemistry
D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D011108:Polymers; D011758:Pyrroles; D013876:Thiophenes; C066730:polythiophene; C067635:polypyrrole; D000069349:Linezolid; D017576:Daptomycin