title: Increased levels of soluble LR11 in cerebrospinal fluid of patients with Alzheimer disease.
abstract: BACKGROUND
  Recent genetic and pathological studies have suggested that a lipoprotein receptor, LR11, is intricately implicated in the pathogenesis of Alzheimer disease (AD). We have recently established a novel sandwich ELISA, which enabled the sensitive quantification of a soluble LR11 (sLR11). By this ELISA, we attempted to determine the difference in the levels of CSF sLR11 in AD patients.
  
  
  METHODS
  We examined CSF from 29 AD patients, 20 frontotemporal lobar degeneration patients and 27 age-matched control subjects. The CSF sLR11 level as well as the levels of tau and beta-amyloid42 (Abeta42) were determined by sandwich ELISA.
  
  
  RESULTS
  The CSF tau level and tau/Abeta42 ratio were significantly increased (p < 0.01) in the AD patients. The CSF sLR11 level in the AD patients was significantly higher (p < 0.01) than that of the frontotemporal lobar degeneration patients and the controls. The APOE-epsilon4-positive AD patients have higher sLR11 levels than the APOE-epsilon4-negative patients (p < 0.01).
  
  
  CONCLUSIONS
  These results suggest that the quantification of CSF sLR11 may serve as a biomarker of AD, although the diagnostic value for individual patients is limited. An elevated CSF sLR11 level in AD patients may be relevant to AD pathogenesis.
authors.affiliation: Department of Molecular Neuroscience, Brain Research Institute, Niigata University, Niigata, Japan. ikeuchi@bri.niigata-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ikeuchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Isamu
authors.initials: I
authors.lastname: Fukamachi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tokutake
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ebinuma
authors.forename: Kohei
authors.initials: K
authors.lastname: Takubo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kaneko
authors.forename: Kensaku
authors.initials: K
authors.lastname: Kasuga
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Kakita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Bujo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Saito
authors.forename: Masatoyo
authors.initials: M
authors.lastname: Nishizawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics; D015415:Biomarkers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D057174:Frontotemporal Lobar Degeneration / Q000134:cerebrospinal fluid; D005838:Genotype; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000134:cerebrospinal fluid*; D008297:Male; D026901:Membrane Transport Proteins / Q000134:cerebrospinal fluid*; D008875:Middle Aged; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D001057:Apolipoproteins E; D015415:Biomarkers; D026502:LDL-Receptor Related Proteins; D026901:Membrane Transport Proteins; C102582:SORL1 protein, human; D016875:tau Proteins