Interaction between ascites susceptibility and CO during the second half of incubation of two broiler lines. Effect on embryonic development and hatching process.
1. Because CO(2) during the second half of incubation is known to influence air cell and blood gases, and embryo development, it is postulated that post-hatch development and ascites sensitivity could also be influenced. 2. An ascites susceptible (A) and an ascites resistant (E) broiler line were incubated under standard incubation or high CO(2) conditions (up to 4%) from embryonic day (ED) 10 onwards. The embryonic development and the hatching process of these two lines were compared when incubated under standard or high CO(2) conditions from over the second half of incubation. 3. The A line, selected for high post-hatch growth rate, exhibited a higher relative embryo weight from ED10 until ED16, which was supported by a higher air cell pCO(2), lower air cell pO(2), higher corticosterone and thyroid hormones and earlier hatching time. 4. Incubation under high CO(2) increased air cell pCO(2), retarded yolk consumption, and decreased glycogen concentration in the liver at hatch. Hatchability decreased in both lines when incubated under high CO(2), due to an increased late mortality of embryos that died before IP. 5. These results suggest that the development and metabolism of CO(2)-incubated embryos differ from control incubated embryos.
Everaert
N
N
Department of Biosystems, K. U. Leuven, Belgium. Nadia.everaert@biw.kuleuven.be
Debonne
M
M
Willemsen
H
H
Witters
A
A
Kamers
B
B
De Baerdemaeker
J
J
Decuypere
E
E
Bruggeman
V
V
D000818:Animals; D001201:Ascites / Q000235:genetics / Q000662:veterinary*; D002245:Carbon Dioxide / Q000494:pharmacology*; D002642:Chick Embryo / Q000187:drug effects*; D002645:Chickens / Q000235:genetics / Q000254:growth & development*; D003345:Corticosterone / Q000097:blood; D047108:Embryonic Development / Q000187:drug effects*; D006003:Glycogen / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D013974:Thyroxine / Q000097:blood; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood
D014284:Triiodothyronine; D002245:Carbon Dioxide; D006003:Glycogen; D013974:Thyroxine; D003345:Corticosterone