title: Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.
abstract: The accurate detection of mycobacterial species from isolates and clinical samples is important for pathogenic diagnosis and treatment and for disease control. There is an urgent need for the development of a rapid, simple, and accurate detection method. We established a biochip assay system, including a biochip, sample preparation apparatus, hybridization instrument, chip washing machine, and laser confocal scanner equipped with interpretation software for automatic diagnosis. The biochip simultaneously identified 17 common mycobacterial species by targeting the differences in the 16S rRNA. The system was assessed with 64 reference strains and 296 Mycobacterium tuberculosis and 243 nontuberculous mycobacterial isolates, as well as 138 other bacteria and 195 sputum samples, and then compared to DNA sequencing. The entire biochip assay took 6 h. The concordance rate between the biochip assay and the DNA sequencing results was 100%. In conclusion, the biochip system provides a simple, rapid, reliable, and highly accurate clinical assay for determination of mycobacterial species in a 6-h procedure, from either culture isolates or sputum samples, allowing earlier pathogen-adapted antimicrobial therapy in patients.
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology, Changping District, Beijing, China.
authors.forename: Lingxiang
authors.initials: L
authors.lastname: Zhu
authors.forename: Guanglu
authors.initials: G
authors.lastname: Jiang
authors.forename: Shengfen
authors.initials: S
authors.lastname: Wang
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
authors.forename: Hairong
authors.initials: H
authors.lastname: Huang
authors.forename: Wanli
authors.initials: W
authors.lastname: Xing
authors.forename: Keith
authors.initials: K
authors.lastname: Mitchelson
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S