[Changes in genes expression caused by overexpression of the transcription factor ZXDC in embryonic kidney cell line HEK293].
The transcription factor ZXDC, a member of ZXD protein family, forms transcriptionally active complex with ZXDA and participates in the regulation of MHCII gene expression. In this work we investigate the expression of a large group of genes in kidney embry cell of line HEK293 with overexpression of transcription factor ZXDC for identification of genes, which transcription is controlled by this transcription factor. We have shown that overexpression of the transcription factor ZXDC in kidney embryo cell of HEK293 line significantly changes the level of expression of large group of genes, which control the behavior of different cell processes, in particular cell cycle, cell proliferation and differentiation. The expression of most of these genes is significantly increased in the kidney cell line HEK293 with overexpression of transcription factor ZXDC, in particular EGR2, BDNF, CDKN1C and IL5Ra genes. Our results clearly demonstrated that transcription factor ZXDC plays a significant role in the regulation of expression of a large group of genes, which control important cell processes.
Halkin
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Minchenko
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D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids; D015534:Trans-Activators / Q000096:biosynthesis* / Q000502:physiology; D014157:Transcription Factors
D018076:DNA, Complementary; D015534:Trans-Activators; D014157:Transcription Factors; C516737:ZXDC protein, human