title: Overexpression of MMP-13 gene in colorectal cancer with liver metastasis.
abstract: Matrix metalloproteinase-7 (MMP-7), MMP-9, MMP-13, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are considered to have important roles in the invasiveness and outcomes of colorectal cancer (CRC). This study examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially as related to liver metastasis. The study analysed surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated colorectal cancer. MMP-7, MMP-9, MMP-13, TIMP-1, and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time, reverse-transcriptase polymerase chain reaction. Expression levels of MMP-7, MMP-9, MMP-13 and TIMP-1 were higher in cancer tissue than in adjacent normal mucosa. On analysis of the relations between gene expression and clinicopathological factors, MMP-13 expression was found to correlate with liver metastasis. Moreover, MMP-13 expression levels were higher in tumour tissue with liver metastasis than in that without liver metastasis. It is concluded that MMP-13 gene expression is a useful predictor of liver metastasis in patients with CRC.
authors.affiliation: Department of Gastrointestinal Surgery, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama-shi, Kanagawa-ken 241-0815, Japan. takay0218@yahoo.co.jp
authors.forename: Takanobu
authors.initials: T
authors.lastname: Yamada
authors.forename: Takashi
authors.initials: T
authors.lastname: Oshima
authors.forename: Kazue
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Shuzo
authors.initials: S
authors.lastname: Tamura
authors.forename: Amane
authors.initials: A
authors.lastname: Kanazawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Inagaki
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sato
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fujii
authors.forename: Kazushi
authors.initials: K
authors.lastname: Numata
authors.forename: Chikara
authors.initials: C
authors.lastname: Kunisaki
authors.forename: Manabu
authors.initials: M
authors.lastname: Shiozawa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Morinaga
authors.forename: Makoto
authors.initials: M
authors.lastname: Akaike
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Rino
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Tanaka
authors.forename: Munetaka
authors.initials: M
authors.lastname: Masuda
authors.forename: Toshio
authors.initials: T
authors.lastname: Imada
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis* / Q000235:genetics; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D053509:Matrix Metalloproteinase 13; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9