title: [Optimal extraction of effective constituents from Aralia elata by central composite design and response surface methodology].
abstract: OBJECTIVE
  To optimize the process of extracting effective constituents from Aralia elata by response surface methodology.
  
  
  METHODS
  The independent variables were ethanol concentration, reflux time and solvent fold, the dependent variable was extraction rate of total saponins in Aralia elata. Linear or no-linear mathematic models were used to estimate the relationship between independent and dependent variables. Response surface methodology was used to optimize the process of extraction. The prediction was carried out through comparing the observed and predicted values.
  
  
  RESULTS
  Regression coefficient of binomial fitting complex model was as high as 0.9617, the optimum conditions of extraction process were 70% ethanol, 2.5 hours for reflux, 20-fold solvent and 3 times for extraction. The bias between observed and predicted values was -2.41%.
  
  
  CONCLUSIONS
  It shows the optimum model is highly predictive.
authors.affiliation: College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. lswa5599@sina.com
authors.forename: Shao-Wa
authors.initials: SW
authors.lastname: Lv
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Hu
authors.forename: Xu-Yan
authors.initials: XY
authors.lastname: Ye
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Xiao
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D027983:Aralia / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012044:Regression Analysis; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D012503:Saponins; D012997:Solvents; D000431:Ethanol