title: Methane production from oleate: assessing the bioaugmentation potential of Syntrophomonas zehnderi.
abstract: The potential for improving long-chain fatty acids (LCFA) conversion to methane was evaluated by bioaugmenting a non-acclimated anaerobic granular sludge with Syntrophomonas zehnderi. Batch bioaugmentation assays were performed with and without the solid microcarrier sepiolite, using 1 mM oleate as sole carbon and energy source. When S. zehnderi was added to the anaerobic sludge methane production from oleate was faster. High methane yields, i.e. 89 ± 5% and 72 ± 1%, were observed in bioaugmented assays in the absence and presence of sepiolite, respectively. Sepiolite stimulated a faster methane production from oleate and prevented the accumulation of acetate. Acetoclastic activity was affected by oleate in the absence of sepiolite, where methane production rate was 26% lower than in assays with microcarrier.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cavaleiro
authors.forename: D Z
authors.initials: DZ
authors.lastname: Sousa
authors.forename: M M
authors.initials: MM
authors.lastname: Alves
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001681:Biological Assay; D019149:Bioreactors / Q000382:microbiology; D008697:Methane / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*
chemical_list: D019301:Oleic Acid; D008697:Methane