Single-walled carbon nanotube chemoresistive label-free immunosensor for salivary stress biomarkers.
The present work is focused on the development, analytical characterization and evaluation of selective and sensitive SWNT-chemiresistor immunosensor for the label-free detection of salivary α-amylase (SAA). SWNTs were aligned to bridge lithographically patterned gold microelectrodes using AC dielectrophoresis followed by functionalization with anti-SAA antibodies. The nano-immunosensors exhibited excellent sensitivity over the clinically relevant range (19 to 308 U ml(-1)) with a limit of detection (LOD) of 6 µg ml(-1) (0.6 U ml(-1)) and 7.8 µg ml(-1) (0.78 U ml(-1)) in phosphate buffer and artificial saliva, respectively, and no interference from other components of saliva. This label-free nano-immunosensor technology has potential application in clinical diagnosis for stress biomarkers expressed in human saliva at the point-of-care.
Tlili
Chaker
C
Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.
Cella
Lakshmi N
LN
Myung
Nosang V
NV
Shetty
Vivek
V
Mulchandani
Ashok
A
D055910:Antibodies, Immobilized / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012463:Saliva / Q000378:metabolism*; D000516:alpha-Amylases / Q000032:analysis*
D055910:Antibodies, Immobilized; D015415:Biomarkers; D037742:Nanotubes, Carbon; D006046:Gold; D000516:alpha-Amylases