title: Neutron capture nuclei-containing carbon nanoparticles for destruction of cancer cells.
abstract: HeLa cells were incubated with neutron capture nuclei (boron-10 and gadolinium)-containing carbon nanoparticles, followed by irradiation of slow thermal neutron beam. Under a neutron flux of 6 x 10(11) n/cm(2) (or 10 min irradiation at a neutron flux of 1 x 10(9) n/cm(2) s), the percentages of acute cell death at 8 h after irradiation are 52, 55, and 28% for HeLa cells fed with BCo@CNPs, GdCo@CNPs, and Co@CNPs, respectively. The proliferation capability of the survived HeLa cells was also found to be significantly suppressed. At 48 h after neutron irradiation, the cell viability further decreases to 35 +/- 5% as compared to the control set receiving the same amount of neutron irradiation dose but in the absence of carbon nanoparticles. This work demonstrates "proof-of-concept" examples of neutron capture therapy using (10)B-, (157)Gd-, and (59)Co-containing carbon nanoparticles for effective destruction of cancer cells. It will also be reported the preparation and surface functionalization of boron or gadolinium doped core-shell cobalt/carbon nanoparticles (BCo@CNPs, GdCo@CNPs and Co@CNPs) using a modified DC pulsed arc discharge method, and their characterization by various spectroscopic measurements, including TEM, XRD, SQUID, FT-IR, etc. Tumor cell targeting ability was introduced by surface modification of these carbon nanoparticles with folate moieties.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu 30043, Taiwan, ROC. kchwang@mx.nthu.edu.tw
authors.forename: Kuo Chu
authors.initials: KC
authors.lastname: Hwang
authors.forename: Po Dong
authors.initials: PD
authors.lastname: Lai
authors.forename: Chi-Shiun
authors.initials: CS
authors.lastname: Chiang
authors.forename: Pei-Jen
authors.initials: PJ
authors.lastname: Wang
authors.forename: Chiun-Jye
authors.initials: CJ
authors.lastname: Yuan
mesh_terms: D001895:Boron / Q000008:administration & dosage / Q000627:therapeutic use*; D016754:Boron Neutron Capture Therapy / Q000379:methods; D002244:Carbon / Q000737:chemistry; D002470:Cell Survival / Q000528:radiation effects; D003035:Cobalt / Q000008:administration & dosage / Q000627:therapeutic use*; D005682:Gadolinium / Q000008:administration & dosage / Q000627:therapeutic use*; D006367:HeLa Cells; D006801:Humans; D007554:Isotopes / Q000008:administration & dosage / Q000627:therapeutic use; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000532:radiotherapy*; D017247:Neutron Capture Therapy / Q000379:methods*
chemical_list: D007554:Isotopes; D003035:Cobalt; D002244:Carbon; D005682:Gadolinium; D001895:Boron