Resveratrol and its oligomers from wine grapes are selective (1)O2 quenchers: mechanistic implication by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry and theoretical calculation.
Resveratrol and its oligomers, abundantly present in wine grapes, are believed to be effective phytoalexins for the phenomenon "French paradox" partially by virtue of their powerful antiradical properties. EPR spin-trapping technique was utilized, demonstrating all polyphenols were selective (1)O2 quenchers but not effective (•)OH and O2(•¯) scavengers. On the basis of the HPLC-ESI-MS(2) analysis for the simulated reactions of polyphenols with (1)O2, the molecular weights of the resulting photochemical products were 14 or 28 Da higher than those of their substrates. No fragment C2H2O (42 Da), which was rather distinctive of the resorcinol rings in these cases, had been observed, whereas their MS/MS spectra displayed characteristic neutral fragments including carbon monoxide (CO, 28 Da) and 2-hydroxy[1,4]benzoquinone (C6H4O3, 124 Da). Finally, PM3 semiempirical calculations and HR-FTICR-MS experiments were performed, supporting the assertion that their quenching mechanism involved physical and chemical pathways. Chemical quenching underwent an endoperoxide intermediate form to generate quinones.
Jiang
Li-Yan
LY
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
He
Shan
S
Jiang
Ke-Zhi
KZ
Sun
Cui-Rong
CR
Pan
Yuan-Jiang
YJ
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D000077185:Resveratrol; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
D011108:Polymers; D013267:Stilbenes; D000077185:Resveratrol; D010100:Oxygen