title: Bone regeneration by bioactive hybrid membrane containing FGF2 within rat calvarium.
abstract: This study examined the bone regeneration potential of a novel hybrid membrane consisting of collagen and nano-bioactive glass (nBG) incorporating basic fibroblast growth factor (FGF2) for use in guided bone regeneration. nBG was added to a reconstitution of collagen at a concentration of 30%, and the hybrid was formulated into a thin membrane. FGF2 (50 microg/ml) was adsorbed to the hybrid membrane. This level of FGF2 was found to be the optimal concentration to stimulate osteoblastic differentiation in vitro. Three membrane groups, including pure collagen, collagen-nBG hybrid and its combination with FGF2 were implanted within a rat calvarium defect (phi = 5 mm) for a period of 3 weeks. Histomorphometric analysis was carried out to evaluate the bone regeneration within the defect. The results showed that the defect in the collagen-nBG-FGF2 membrane was recovered almost completely, while partial recovery was observed in the other membrane groups (collagen and collagen-BG). However, there was little defect recovery in the blank control. The new bone formation was as high as approximately 60, approximately 45, and approximately 30% of the defect treated with the collagen-nBG-FGF2, collagen-BG, and collagen, respectively, whilst only 4% of new bone was observed in the blank control. Overall, the nBG was shown to stimulate bone formation of the collagen membrane, and FGF2 synergistically accelerated the bone regeneration within a rat calvarium defect.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea.
authors.forename: Ki Seok
authors.initials: KS
authors.lastname: Hong
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
authors.forename: So-Hee
authors.initials: SH
authors.lastname: Bang
authors.forename: Chin-Hyung
authors.initials: CH
authors.lastname: Chung
authors.forename: Young Il
authors.initials: YI
authors.lastname: Lee
authors.forename: Jung Keun
authors.initials: JK
authors.lastname: Hyun
authors.forename: Hae-Hyoung
authors.initials: HH
authors.lastname: Lee
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Kim
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001681:Biological Assay; D001861:Bone Regeneration / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects* / Q000473:pathology / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D016222:Fibroblast Growth Factor 2