title: Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats.
abstract: The syncytiotrophoblast, which regulates maternal-fetal transfer of drugs, consists of a single layer in humans, but two layers, i.e., SynI and SynII, in rodents. Polar distribution of transporters in the apical and basal plasma membranes of syncytiotrophoblast is important for placental function in terms of vectorial transport of substrates, but the mechanisms that control protein distribution in the syncytiotrophoblast remain unclear. We have previously established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, which retain characteristics of SynI and SynII, respectively. In this study, we aimed to characterize the gene expression profiles in the two layers by using these cell lines. DNA microarray analysis indicated that more than 25 mRNAs, including cytoskeleton binding proteins, ezrin and CLP36, are differentially expressed between TR-TBT 18d-1 and TR-TBT 18d-2. Quantitative real time-polymerase chain reaction (PCR) analysis indicated that mRNA expression of ezrin, CLP36, CCN1, and CCN2 is higher in TR-TBT 18d-1 and mRNA expression of elf-1a, hsc70 and flot2 is higher in TR-TBT 18d-2, compared with their counterparts. Immunohistochemical analysis indicated that ezrin is expressed in rat placental villi in vivo, and is located on the apical membranes of TR-TBT 18d-1, while CLP36 is located in the apical and basal sides of TR-TBT 18d-1. The expression of ezrin was highest at gestational days 14 and 18 and was highest among the ezrin/radixin/moesin (ERM) family members. These results may help to clarify the mechanisms controlling polarization of the syncytiotrophoblast and the significance of the double epithelial layers in rat and mouse.
authors.affiliation: Division of Pharmaceutics, Faculty of Pharmacy, Keio University, Tokyo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Higuchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iizasa
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Sai
authors.forename: Satomi
authors.initials: S
authors.lastname: Horieya
authors.forename: Kyeong-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Masami
authors.initials: M
authors.lastname: Wada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Deguchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nishimura
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukita
authors.forename: Noriko
authors.initials: N
authors.lastname: Kose
authors.forename: Young-Sook
authors.initials: YS
authors.lastname: Kang
authors.forename: Emi
authors.initials: E
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D005865:Gestational Age; D018398:Homeodomain Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D060588:LIM Domain Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; D000099149:Ezrin; C097168:Pdlim1 protein, rat