title: The peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica.
abstract: In this paper, we describe the CTA1 gene, which encodes a peroxisomal catalase in the methylotrophic yeast Pichia methanolica. The P. methanolica CTA1 gene (PmCTA1) comprises a 1,530-bp open reading frame corresponding to a protein of 510 amino acid residues, and its deduced amino acid sequence shows high similarity to those of Cta1ps from other methylotrophic yeasts (about 79%). Expression of PmCTA1 in a peroxisomal catalase-depleted (Cbcta1Delta) Candida boidinii strain restored the methylotrophic growth of the host strain, while the expression of PmCTA1-DeltaSRL, which lacks peroxisome targeting signal type 1, did not. In P. methanolica, expression of PmCTA1 was induced when cells were grown on peroxisome-inducing carbon sources, viz., methanol, oleate, and D-alanine. Taken together, these results indicate that PmCTA1 encodes a functional peroxisomal catalase in P. methanolica.
authors.affiliation: Faculty of Applied Biological Science, Gifu University, Yanagido, Gifu, Japan. t-nakaga@gifu-u.ac.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yoshida
authors.forename: Akihito
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Shuki
authors.initials: S
authors.lastname: Fujimura
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsufuji
authors.forename: Noboru
authors.initials: N
authors.lastname: Tomizuka
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yurimoto
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000595:Amino Acid Sequence; D002374:Catalase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000201:enzymology*; D010843:Pichia / Q000201:enzymology* / Q000235:genetics*
chemical_list: D002374:Catalase