Identifying genes progressively silenced in preneoplastic and neoplastic liver tissues.
High-throughput genomic technologies are increasingly being used to identify therapeutic targets and risk factors for specific diseases. Using 116 independent liver samples, we identified 793 probe sets that demonstrated a significant association in the frequency of absent calls as tissues progressed from normal to pre-neoplastic to neoplastic, followed by a bioinformatic approach which identified that 78.9% of the significant probe sets contained at least one CpG island in the gene promoter region compared with 58.9% of the remaining genes examined. Our results indicate that further high-throughput methylation studies to more fully characterize molecular events involved in hepatocarcinogenesis are warranted.
Archer
Kellie J
KJ
Department of Biostatistics, Virginia Commonwealth University, Richmond, Virginia 23298-0032, USA. kjarcher@vcu.edu
Zhao
Zhongming
Z
Guennel
Tobias
T
Maluf
Daniel G
DG
Fisher
Robert A
RA
Mas
Valeria R
VR
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology / Q000379:methods; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D023281:Genomics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D011230:Precancerous Conditions / Q000235:genetics; D011401:Promoter Regions, Genetic; D012307:Risk Factors