Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan).
The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T. evansi genomic DNA. The samples were screened for the presence of T. evansi also by stained thin smear. Forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were identified by PCR, indicating the high sensitivity of PCR for surveying the disease in epidemiological studies.
Shahzad
Waseem
W
Livestock Production Research Institute Bahadurnagar, Okara, Punjab, Pakistan. waseem1971@hotmail.com
Munir
Rashid
R
Khan
Mohammad S
MS
Ahmad
Mansur D
MD
Ijaz
Mohammad
M
Ahmad
Ashfaq
A
Iqbal
Mohammad
M
D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D008297:Male; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000235:genetics*; D014352:Trypanosomiasis / Q000453:epidemiology* / Q000662:veterinary*
D017931:DNA Primers