Phase 1 of an in vitro neurotoxicological pre-validation trial.
In the absence of a formal in vitro alternative model for neurotoxicity testing, the CellTox Centre, in collaboration with the University of Salford, FRAME and the EEC, has set up an initial pre-validation trial of a three-tiered scheme that was originally proposed in 1989. Using a cell battery of neuroblastomas and primary neural cultures (Tier I) and primary astrocytes (Tier III) an initial set of 20 chemicals (out of a reference set of 40 chemicals) has been assessed for neurotoxicity using a multiple end point assay system. The methods used were relatively simple, rapid and inexpensive, and the chemical end points were easily quantified. The results of the pre-validation trial have enabled us to revise the first tier of the proposed tiered system for full micro-validation.
Williams
S P
SP
CellTox Centre, Division of Biosciences, University of Hertfordshire, College Lane, Hatfield, Herts. AL10 9AB, UK.
Davenport-Jones
J
J
Egan
C
C
O'Hare
S
S
Cookson
M
M
McClean
R
R
Garle
M J
MJ
Pentreath
V
V
Atterwill
C K
CK