Establishment of immortal multipotent rat salivary progenitor cell line toward salivary gland regeneration.
Adult salivary gland stem cells are promising candidates for cell therapy and tissue regeneration in cases of irreversible damage to salivary glands in head and neck cancer patients undergoing irradiation therapy. At present, the major restriction in handling such cells is their relatively limited life span during in vitro cultivation, resulting in an inadequate experimental platform to explore the salivary gland-originated stem cells as candidates for future clinical application in therapy. We established a spontaneous immortal integrin α6β1-expressing cell line of adult salivary progenitor cells from rats (rat salivary clone [RSC]) and investigated their ability to sustain cellular properties. This line was able to propagate for more than 400 doublings without loss of differentiation potential. RSC could differentiate in vitro to both acinar- and ductal-like structures and could be further manipulated upon culturing on a 3D scaffolds with different media supplements. Moreover, RSC expressed salivary-specific mRNAs and proteins as well as epithelial stem cell markers, and upon differentiation process their expression was changed. These results suggest RSC as a good model for further studies exploring cellular senescence, differentiation, and in vitro tissue engineering features as a crucial step toward reengineering irradiation-impaired salivary glands.
Yaniv
Adi
A
1 Faculty of Dental Medicine, Institute of Dental Sciences, The Hebrew University of Jerusalem , Jerusalem, Israel .
Neumann
Yoav
Y
David
Ran
R
Stiubea-Cohen
Raluca
R
Orbach
Yoav
Y
Lang
Stephan
S
Rotter
Nicole
N
Dvir-Ginzberg
Mona
M
Aframian
Doron J
DJ
Palmon
Aaron
A
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D016922:Cellular Senescence; D004847:Epithelial Cells / Q000166:cytology; D005434:Flow Cytometry; D018189:Immunomagnetic Separation; D039121:Integrin alpha6beta1 / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D039902:Multipotent Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods
D039121:Integrin alpha6beta1; D012333:RNA, Messenger