title: The estimation of oxidative stress markers and apoptosis in right atrium auricles cardiomyocytes of patients undergoing surgical heart revascularisation with the use of warm blood cardioplegia.
abstract: Oxidative stress markers and apoptosis were estimated during elective surgical heart revascularization. Eight patients with good ejection fraction underwent coronary artery bypass grafting (CABG) with the use of warm blood cardioplegia. Two right atrium auricle biopsy specimens were collected before and after the operation. Specimens underwent immunocytochemical analysis of mitochondrial manganese superoxide dismutase (MnSOD) expression and apoptosis estimation by the TUNEL method. Ultrastructure analysis under electron microscope was made. Satisfactory results of the operation were obtained. After CABG the MnSOD expression increase in sections of auricles was observed through the increase of stain intensity and the percentage of cells with positive stain (from 30 to 80%). The apoptotic cells percentage remained at approximately the same level. Under the electron microscope insignificant pathological changes were observed. On this basis one may assume that in the case of cardiosurgical procedures with short aorta cross-clamping time and low operation risk level the application of cardioplegia sufficiently prevents reactive oxygen forms (ROF) cytotoxic activity although it does not inhibit the expression of oxidative stress (OS) markers. In our opinion the method of examining right atrium sections is safe and provides results comparable with other publications. It may also be a voice in the discussion on new methods of heart protection during cardiac surgery procedures.
authors.affiliation: Department of Cardiac Surgery, Medical University of Wroclaw, Poland. ravnov@wp.pl
authors.forename: Rafal
authors.initials: R
authors.lastname: Nowicki
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
authors.forename: Anna
authors.initials: A
authors.lastname: Choromanska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Dumanska
authors.forename: Barbara
authors.initials: B
authors.lastname: Krajewska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Daczewska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dumanski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kustrzycki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000502:physiology*; D020517:Atrial Appendage / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D015415:Biomarkers / Q000378:metabolism*; D002314:Cardioplegic Solutions / Q000378:metabolism; D001026:Coronary Artery Bypass / Q000379:methods; D005260:Female; D006324:Heart Arrest, Induced / Q000379:methods*; D006325:Heart Atria / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D009204:Myocardial Revascularization / Q000379:methods*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000648:ultrastructure; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002314:Cardioplegic Solutions; D013482:Superoxide Dismutase