title: Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.
abstract: A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.
authors.affiliation: School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Suk-Chae
authors.initials: SC
authors.lastname: Jung
authors.forename: Chris L
authors.initials: CL
authors.lastname: Smith
authors.forename: Ki-Sung
authors.initials: KS
authors.lastname: Lee
authors.forename: Min-Eui
authors.initials: ME
authors.lastname: Hong
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kweon
authors.forename: Gregory
authors.initials: G
authors.lastname: Stephanopoulos
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Jin
mesh_terms: D000264:Adenylosuccinate Lyase / Q000235:genetics*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D005816:Genetic Complementation Test; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D017354:Point Mutation / Q000235:genetics
chemical_list: D003470:Culture Media; D000264:Adenylosuccinate Lyase