Application of polycaprolactone as an anti-adhesion biomaterial film.
Adhesions are unavoidable consequences of surgery and other trauma. How to prevent the adhesions remains a big issue in healthcare system. The objective of this study is to test the efficacy of polycaprolactone (PCL) films as physical barriers in reducing postoperative intra-abdominal adhesions in the rat cecum-abdominal wall model. PCL is quite cheap compared with the agents recently used in the market. The fabrication method is also very easy to perform. Scanning electron microscope (SEM) showed multiple pores over upper and bottom surfaces but too small to permit cells to migrate from one surface onto another surface. Those pores were proven to be not interconnected. The PCL film did not show any evidence of cytotoxic effects as it did not induce any significant increase in cytoplasmic lactate dehydrogenase release from the NIH3T3 cells that it came in contact with. In animal studies, the PCL films led to fewer adhesions than Seprafilm in rat adhesion model. PCL films were efficacious in reducing postoperative intra-abdominal adhesion formation in rat cecum-abdominal wall models.
Lo
Hsien-Yi
HY
Institute of Biomedical Engineering, College of Medicine, College of Engineering, National Taiwan University, Taiwan.
Kuo
Hsuan-Ting
HT
Huang
Yi-You
YY
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000627:therapeutic use* / Q000633:toxicity; D006820:Hyaluronic Acid / Q000627:therapeutic use; D007770:L-Lactate Dehydrogenase; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000138:chemical synthesis / Q000627:therapeutic use* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D000267:Tissue Adhesions / Q000517:prevention & control*
D001672:Biocompatible Materials; D011091:Polyesters; C118198:Seprafilm; C016240:polycaprolactone; D006820:Hyaluronic Acid; D007770:L-Lactate Dehydrogenase