title: Archaea signal recognition particle shows the way.
abstract: Archaea SRP is composed of an SRP RNA molecule and two bound proteins named SRP19 and SRP54. Regulated by the binding and hydrolysis of guanosine triphosphates, the RNA-bound SRP54 protein transiently associates not only with the hydrophobic signal sequence as it emerges from the ribosomal exit tunnel, but also interacts with the membrane-associated SRP receptor (FtsY). Comparative analyses of the archaea genomes and their SRP component sequences, combined with structural and biochemical data, support a prominent role of the SRP RNA in the assembly and function of the archaea SRP. The 5e motif, which in eukaryotes binds a 72 kilodalton protein, is preserved in most archaea SRP RNAs despite the lack of an archaea SRP72 homolog. The primary function of the 5e region may be to serve as a hinge, strategically positioned between the small and large SRP domain, allowing the elongated SRP to bind simultaneously to distant ribosomal sites. SRP19, required in eukaryotes for initiating SRP assembly, appears to play a subordinate role in the archaea SRP or may be defunct. The N-terminal A region and a novel C-terminal R region of the archaea SRP receptor (FtsY) are strikingly diverse or absent even among the members of a taxonomic subgroup.
authors.affiliation: Department of Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708-3154, USA. zwieb@uthct.edu
authors.forename: Christian
authors.initials: C
authors.lastname: Zwieb
authors.forename: Shakhawat
authors.initials: S
authors.lastname: Bhuiyan
mesh_terms: D001105:Archaea / Q000201:enzymology*; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D008954:Models, Biological; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019642:RNA, Archaeal / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020733:RNA, Small Cytoplasmic / Q000737:chemistry / Q000235:genetics; D018271:Signal Recognition Particle / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: C115990:7SL RNA; D019843:Archaeal Proteins; D019642:RNA, Archaeal; D020733:RNA, Small Cytoplasmic; D018271:Signal Recognition Particle