title: Murine bioluminescent hepatic tumour model.
abstract: This video describes the establishment of liver metastases in a mouse model that can be subsequently analysed by bioluminescent imaging. Tumour cells are administered specifically to the liver to induce a localised liver tumour, via mobilisation of the spleen and splitting into two, leaving intact the vascular pedicle for each half of the spleen. Lewis lung carcinoma cells that constitutively express the firefly luciferase gene (luc1) are inoculated into one hemi-spleen which is then resected 10 minutes later. The other hemi-spleen is left intact and returned to the abdomen. Liver tumour growth can be monitored by bioluminescence imaging using the IVIS whole body imaging system. Quantitative imaging of tumour growth using IVIS provides precise quantitation of viable tumour cells. Tumour cell death and necrosis due to drug treatment is indicated early by a reduction in the bioluminescent signal. This mouse model allows for investigating the mechanisms underlying metastatic tumour-cell survival and growth and can be used for the evaluation of therapeutics of liver metastasis.
authors.affiliation: Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, Ireland.
authors.forename: Simon
authors.initials: S
authors.lastname: Rajendran
authors.forename: Slawomir
authors.initials: S
authors.lastname: Salwa
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Gao
authors.forename: Sabin
authors.initials: S
authors.lastname: Tabirca
authors.forename: Deirdre
authors.initials: D
authors.lastname: O'Hanlon
authors.forename: Gerald C
authors.initials: GC
authors.lastname: O'Sullivan
authors.forename: Mark
authors.initials: M
authors.lastname: Tangney
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000473:pathology / Q000556:secondary; D004195:Disease Models, Animal; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000556:secondary*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D009368:Neoplasm Transplantation / Q000379:methods*; D013154:Spleen / Q000601:surgery