[Study on the longevity related mitochondrial genome variation in Bama elderly population in Guangxi province].
OBJECTIVE
To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi.


METHODS
Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism (SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi.


RESULTS
The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls. And it was significantly higher in controls than that in long-lived population (P<0.05).


CONCLUSIONS
The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.
Lv
Ze-ping
ZP
Guangxi Jiangbin Hospital, Nanning, Guangxi, 530021 PR China. lvzeping@163.com
Zheng
Chen-guang
CG
Kong
Fang
F
Feng
Jie
J
Jiang
Wen-yu
WY
Hu
Cai-you
CY
Li
Hua
H
Lv
Yuan
Y
Zhang
Gen-fa
GF
Yang
Ze
Z
D000368:Aged; D002681:China / Q000208:ethnology; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008136:Longevity / Q000235:genetics*; D009154:Mutation; D002052:Myanmar / Q000208:ethnology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044382:Population Groups
D004272:DNA, Mitochondrial