Pt-NiCo nanostructures with facilitated electrocatalytic activities for sensitive determination of intracellular thiols with long-term stability.
A Pt-NiCo nanomaterial has been synthesized for developing the sensitive electrochemical determination of biological thiols that include L-cysteine (CySH), homocysteine (HCySH), and gluthione (GSH) with high sensitivity and long-term stability, in which the Pt nanoparticles are well supported on amorphous NiCo nanofilms. The electrochemical oxidation of thiols has been successfully facilitated on the optimized Pt-NiCo nanostructures, that is, two oxidation peaks of CySH have been clearly observed at potentials of +0.06 and +0.45 V. The experimental results demonstrate that the first peak for CySH oxidation may be attributed to a direct oxidation from CySH to L-cystine (CySSCy), whereas the second peak possibly results from a sequential oxidation from CySSCy to cysteic acid (CySO(3)H), together with a direct oxidation of CySH into CySO(3)H. The enhanced electrocatalytic activities at the Pt(23)-NiCo nanostructures have provided a methodology to determine thiols at a very low potential of 0.0 V with relatively high sensitivity (637 nA μM cm(-2)), a low detection limit (20 nM), and a broad linear range. The striking analytical performance, together with the characteristic properties of the Pt-NiCo nanomaterial itself, including long-term stability and strong antipoisoning ability, has established a reliable and durable approach for the detection of thiols in liver cancer cells, Hep G2.
Zhang
Feng
F
Department of Chemistry, Tongji University, Shanghai 200092, P.R. China.
Wen
Ming
M
Cheng
Mingzhu
M
Liu
Di
D
Zhu
Anwei
A
Tian
Yang
Y
D015374:Biosensing Techniques / Q000379:methods*; D003545:Cysteine / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry*; D056945:Hep G2 Cells / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry*
D013438:Sulfhydryl Compounds; D010984:Platinum; D009532:Nickel; D005978:Glutathione; D003545:Cysteine