title: Apoptotic effects of polyprenylated benzoylphloroglucinol derivatives from the twigs of Garcinia multiflora.
abstract: With bioassay-guided fractionation, five new polyprenylated benzoylphloroglucinol derivatives, garcimultiflorone D (1), 18-hydroxygarcimultiflorone D (2), garcimultiflorone E (3), garcimultiflorone F (4), and isogarcimultiflorone F (5), and five known compounds, guttiferone E (6), guttiferone F (7), aristophenone A (8), isoxanthochymol (9), and morelloflavone (10), were isolated from the acetone extract of the twigs of Garcinia multiflora. The compounds were evaluated for their apoptotic effects against HeLa-C3 cells, which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. Compounds 1 and 3-9 activate caspase-3 in HeLa-C3 cells within 72 h after treatment at a concentration of 100 microM or lower. In particular, compounds 6, 8, and 9 showed strong apoptosis-inducing effects at a concentration of 25 microM.
authors.affiliation: Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, NT, Hong Kong, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Qiao
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Chen
authors.forename: Kathy Qian
authors.initials: KQ
authors.lastname: Luo
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029760:Garcinia / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010696:Phloroglucinol