Quantitative detection of DNA by autocatalytic enlargement of hybridized gold nanoprobes.
Quantitative detection of specific viral DNA has become a pressing issue for the earlier clinical diagnosis of viral infectious diseases. Therefore, in this paper, we report a simple, sensitive, and inexpensive quantitative approach for DNA detection based on the autocatalytic Au deposition of gold nanoprobes via the surface reduction of AuCl4- to Au0 on their surface in the presence of ascorbic acid (AA) and cetyltrimethylammonium bromide (CTAB). On this basis, signal enhancements in the absorbance intensity and kinetic behavior of gold enlargement in the aqueous phase have been well investigated and explained for the selection of analytical parameters. To achieve high sensitivity, magnetic particles conjugated with capture probes (PMPs) were employed for the collection of gold nanoprobes. After denaturated by ion a pH 11 solution, the amplified signals of gold nanoprobes, which is proportional to the concentration of the target DNA, could easily be confirmed by a UV-vis scanning spectrophotometer. Limit of detection could be obtained as low as 1.0 fM by this simple method.
Zhan
Zongrui
Z
Nano-optics and Biomolecular Engineering National Laboratory, Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Cao
Cuong
C
Sim
Sang Jun
SJ
D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D017403:In Situ Hybridization / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
D006046:Gold; D004247:DNA