title: Stroke status evoked adhesion molecule genetic alterations in astrocytes isolated from stroke-prone spontaneously hypertensive rats and the apigenin inhibition of their expression.
abstract: We examined the possibility that the expression of adhesion molecules is regulated differently in cultured astrocytes from stroke-prone spontaneously hypertensive rats (SHRSP/IZM) rats than in those from Wistar Kyoto rats (WKY/IZM) by tumor necrosis factor-alpha (TNF-alpha) or hypoxia and reoxygenation (H/R) and the inhibitory effects of apigenin. It was found that the expression of vascular cell adhesion molecule-1 (VCAM-1) by TNF-alpha in astrocytes isolated from SHRSP/IZM was increased compared with that in WKY/IZM. The expression of monocyte chemotactic protein-1 (MCP-1) mRNA induced by H/R in SHRSP/IZM astrocytes was increased compared with that in normal oxygen concentrations. Apigenin strongly attenuated TNF-alpha-induced VCAM-1 mRNA and protein expression and suppressed the adhesion of U937 cells and SHRSP/IZM astrocytes. These results suggest that the expression levels of adhesion molecules during H/R affect disease outcome and can drive SHRSP/IZM to stroke. It is suggested that apigenin regulates adhesion molecule expression in reactive astrocytes during ischemia.
authors.affiliation: Department of Food Science and Technology, College of Bioresource Sciences, Nihon University (NUBS), Kanagawa 252-8510, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamagata
authors.forename: Takuya
authors.initials: T
authors.lastname: Kitazawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shinoda
authors.forename: Chika
authors.initials: C
authors.lastname: Tagawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Chino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsufuji