title: Mutagenic potency of Helicobacter pylori in the gastric mucosa of mice is determined by sex and duration of infection.
abstract: Helicobacter pylori is a human carcinogen, but the mechanisms evoked in carcinogenesis during this chronic inflammatory disease remain incompletely characterized. We determined whether chronic H. pylori infection induced mutations in the gastric mucosa of male and female gpt delta C57BL/6 mice infected for 6 or 12 mo. Point mutations were increased in females infected for 12 mo. The mutation frequency in this group was 1.6-fold higher than in uninfected mice of both sexes (P < 0.05). A:T-to-G:C transitions and G:C-to-T:A transversions were 3.8 and 2.0 times, respectively, more frequent in this group than in controls. Both mutations are consistent with DNA damage induced by oxidative stress. No increase in the frequency of deletions was observed. Females had more severe gastric lesions than males at 6 mo postinfection (MPI; P < 0.05), but this difference was absent at 12 MPI. In all mice, infection significantly increased expression of IFNgamma, IL-17, TNFalpha, and iNOS at 6 and 12 mo, as well as H. pylori-specific IgG1 levels at 12 MPI (P < 0.05) and IgG2c levels at 6 and 12 MPI (P < 0.01 and P < 0.001). At 12 MPI, IgG2c levels in infected females were higher than at 6 MPI (P < 0.05) and also than those in infected males at 12 MPI (P < 0.05). Intensity of responses was mediated by sex and duration of infection. Lower H. pylori colonization indicated a more robust host response in females than in males. Earlier onset of severe gastric lesions and proinflammatory, Th1-biased responses in female C57BL/6 mice may have promoted mutagenesis by exposing the stomach to prolonged oxidative stress.
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Sheh
authors.forename: Chung Wei
authors.initials: CW
authors.lastname: Lee
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masumura
authors.forename: Barry H
authors.initials: BH
authors.lastname: Rickman
authors.forename: Takehiko
authors.initials: T
authors.lastname: Nohmi
authors.forename: Gerald N
authors.initials: GN
authors.lastname: Wogan
authors.forename: James G
authors.initials: JG
authors.lastname: Fox
authors.forename: David B
authors.initials: DB
authors.lastname: Schauer
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016207:Cytokines / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D005756:Gastritis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D015870:Gene Expression; D016481:Helicobacter Infections / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016296:Mutagenesis; D009154:Mutation; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D018384:Oxidative Stress; D010430:Pentosyltransferases / Q000235:genetics; D012727:Sex Characteristics; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D010430:Pentosyltransferases; C071962:Gpt protein, E coli