Comparison of estrogens and estrogen metabolites in human breast tissue and urine.
BACKGROUND
An important aspect of the link between estrogen and breast cancer is whether urinary estrogen levels are representative of the intra-tissue levels of bioavailable estrogens.


METHODS
This study compares 15 estrogen and estrogen metabolite levels in breast tissue and urine of 9 women with primary breast cancer using a quantitative liquid chromatography-mass spectrometry method.


RESULTS
The average levels of estrogens (estrone, 17 beta-estradiol) were significantly higher in breast tissue than in urine. Both the 2 and the 16-hydroxylation pathways were less represented in breast tissue than urine; no components of the 4-hydroxypathway were detected in breast tissue, while 4-hydroxyestrone was measured in urine. However, the 2/16 ratio was similar in urine and breast tissue. Women carrying the variant CYP1B1 genotype (Leu/Val and Val/Val) showed significantly lower overall estrogen metabolite, estrogen, and 16-hydroxylation pathway levels in breast tissue in comparison to women carrying the wild type genotype. No effect of the CYP1B1 polymorphism was observed in urinary metabolites.


CONCLUSIONS
The urinary 2/16 ratio seems a good approximation of the ratio observed in breast tissue. Metabolic genes may have an important role in the estrogen metabolism locally in tissues where the gene is expressed, a role that is not readily observable when urinary measurements are performed.
Taioli
Emanuela
E
SUNY Downstate Medical Center, Brooklyn, New York 11203, USA. emanuela.taioli@downstate.edu
Im
Annie
A
Xu
Xia
X
Veenstra
Timothy D
TD
Ahrendt
Gretchen
G
Garte
Seymour
S
D000328:Adult; D000368:Aged; D001189:Aryl Hydrocarbon Hydroxylases; D001940:Breast / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000652:urine; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000652:urine; D016022:Case-Control Studies; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism* / Q000652:urine*; D004967:Estrogens / Q000378:metabolism* / Q000652:urine*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007930:Leucine / Q000235:genetics; D053858:Metabolic Networks and Pathways / Q000235:genetics / Q000502:physiology; D055442:Metabolome; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D014633:Valine / Q000235:genetics
D004967:Estrogens; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1; D007930:Leucine; D014633:Valine