Application of power functions to chromatographic data for the enhancement of signal to noise ratios and separation resolution.
Chromatographic detection responses are recorded digitally. A peak is represented ideally by a Guassian distribution. Raising a Guassian distribution to the power 'n' increases the height of the peak to that power, but decreases the standard deviation by radicaln. Hence there is an increasing disparity in detection responses as the signal moves from low level noise, with a corresponding decrease in peak width. This increases the S/N ratio and increases peak to peak resolution. The ramifications of these factors are that poor resolution in complex chromatographic data can be improved, and low signal responses embedded at near noise levels can be enhanced. The application of this data treatment process is potentially very useful in 2D-HPLC where sample dilution occurs between dimension, reducing signal response, and in the application of post-reaction detection methods, where band broadening is increased by virtue of reaction coils. In this work power functions applied to chromatographic data are discussed in the context of (a) complex separation problems, (b) 2D-HPLC separations, and (c) post-column reaction detectors.
Shalliker
R A
RA
Australian Centre for Research on Separation Science, University of Western Sydney, South Penrith, NSW, Australia. r.shalliker@uws.edu.au
Stevenson
P G
PG
Shock
D
D
Mnatsakanyan
M
M
Dasgupta
P K
PK
Guiochon
G
G
D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008956:Models, Chemical; D016011:Normal Distribution; D010851:Picrates / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D014992:Xylenes / Q000737:chemistry
D001713:Biphenyl Compounds; D010851:Picrates; D014992:Xylenes; C004931:1,1-diphenyl-2-picrylhydrazyl