Nontoxic genetic engineering of mesenchymal stem cells using serum-compatible pullulan-spermine/DNA anioplexes.
Genetic modification of stem cells could be applied to initiate/enhance their secretion of therapeutic molecules, alter their biological properties, or label them for in vivo tracking. We recently developed a negatively charged gene carrier ("anioplex") based on pullulan-spermine, a conjugate prepared from a natural polysaccharide and polyamine. In rat mesenchymal stem cells (MSCs), anioplex-derived reporter gene activity was comparable to or exceeded that obtained using a commercial cationic lipid reagent. Transfection in the growth medium with 15% serum and antibiotics was approximately sevenfold more effective than in serum-free conditions. Cytotoxicity was essentially indiscernible after 24 h of anioplex transfection with 20 μg/mL DNA, in contrast to cationic lipid transfection that resulted in 40%-60% death of target MSCs. Anioplex-derived reporter gene activity persisted throughout the entire 3-week study, with post-transfection MSCs appearing to maintain osteogenic, adipogenic, and chondrogenic multipotency. In particular, chondrogenic pellet formation of differentiating human MSCs was significantly inhibited after lipofection but not after aniofection, which further indicates the biological inertness of pullulan-spermine/DNA anioplexes. Collectively, these data introduce a straightforward technology for genetic engineering of adult stem/progenitor cells under physiological niche-like conditions. Moreover, reporter gene activity was observed in rat spinal cords after minimally invasive intrathecal implantation, suggesting effective engraftment of donor MSCs. It is therefore plausible that anioplex-transfected MSCs or other stem/progenitor cells with autologous potential could be applied to disorders such as neurotrauma or neuropathic pain that involve the spinal cord and brain.
Thakor
Devang K
DK
Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Teng
Yang D
YD
Obata
Hideaki
H
Nagane
Kentaro
K
Saito
Shigeru
S
Tabata
Yasuhiko
Y
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D016923:Cell Death; D002454:Cell Differentiation; D002470:Cell Survival; D004247:DNA / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D005936:Glucans / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*; D013116:Spinal Cord / Q000473:pathology; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D014162:Transfection
D005936:Glucans; D013096:Spermine; C009109:pullulan; D004247:DNA