The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress.
NifS-like cysteine desulfurases are widespread enzymes involved in the mobilization of sulfur from cysteine. The genome of the filamentous diazotrophic cyanobacterium Anabaena PCC 7120 contains four open reading frames potentially encoding NifS-like proteins. One of them, alr2505, belongs to the pkn22 operon, which enables Anabaena to cope with oxidative stress. The Alr2505 protein was purified and found to share all the features characteristic of cysteine desufurases. This is the first NifS-like enzyme to be functionally characterized in this bacterium. On the basis of the transcriptional profiling of all nifS-like genes in Anabaena, it is concluded that alr2505 is the only cysteine desulfurase-encoding gene induced by oxidative stress. The function of Alr2505, which was termed OsiS, is discussed.
Ruiz
Marion
M
Aix-Marseille Université and Laboratoire de Chimie Bactérienne, IBSM-CNRS, Marseille, France.
Bettache
Azzeddine
A
Janicki
Annick
A
Vinella
Daniel
D
Zhang
Cheng-Cai
CC
Latifi
Amel
A
D017033:Anabaena / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D020134:Catalytic Domain / Q000235:genetics; D016384:Consensus Sequence; D019281:Dimerization; D004790:Enzyme Induction; D005288:Ferredoxins / Q000378:metabolism; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D009876:Operon / Q000235:genetics; D018384:Oxidative Stress; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D005288:Ferredoxins; D011994:Recombinant Proteins; C026518:apoferredoxin; C080001:nifS protein, Bacteria; D013437:Carbon-Sulfur Lyases; C080546:cysteine desulfurase