Effect of primate bone marrow stromal cells on survival and neurite outgrowth.
We tested whether bone marrow stromal cells (BMSCs) could enhance the survival and neurite growth of dorsal root ganglia (DRG) through substrate effects or secreted factors. Our results showed that in DRG with BMSCs and BMSC-conditioned media cultures compared with DRG-fibroblast cultures, there was a significant increase in the number and length of, area covered by, and number of cells with definite neurites. In cytokine assays with conditioned media, vascular endothelial growth factor, granulocyte macrophage colony-stimulating factor, and IL-6 secreted by BMSCs may contribute to observed neurotrophic effects. These findings indicate that BMSCs of adult Macaca fascicularis increased neuronal survival and promoted neurite outgrowth of DRG by means of secretory factors.
Kim
Keung Nyun
KN
Department of Neurological Surgery, Yonsei University College of Medicine, Yonsei University, Seoul, Korea.
Guest
James D
JD
Oh
Jin Soo
JS
Pennant
William A
WA
Yoon
Do Heum
DH
Ha
Yoon
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002452:Cell Count; D002470:Cell Survival / Q000502:physiology; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D005727:Ganglia, Spinal / Q000166:cytology; D007150:Immunohistochemistry; D008252:Macaca fascicularis; D008297:Male; D016501:Neurites / Q000502:physiology*; D017154:Stromal Cells / Q000637:transplantation*
D017077:Culture Media, Conditioned; D016207:Cytokines