[Molecular characterization and evolutional analysis of liportein lipase and hepatic lipase gene in Chinese Sturgeon and other six freshwater fishes].
In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.
Huang
Yan
Y
College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Liang
Xu-Fang
XF
Wang
Lin
L
Li
Guang-Zhao
GZ
Liu
Xiu-Xia
XX
Yao
Yu
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps; D006224:Cricetinae / Q000145:classification / Q000235:genetics*; D003412:Cricetulus / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D019143:Evolution, Molecular; D005399:Fishes / Q000145:classification / Q000235:genetics*; D005618:Fresh Water; D008049:Lipase / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D008049:Lipase; D008071:Lipoprotein Lipase