Development of novel lipidated analogs of picroside as vaccine adjuvants: acylated analogs of picroside-II elicit strong Th1 and Th2 response to ovalbumin in mice.
The acylated analogs of picroside-II were synthesized and tested for immune-adjuvant activity in the presence of weak antigen ovalbumin found to stimulate anti-OVA IgG titer, neutralizing antibody (IgG1 and IgG2a) titer as well as the production of soluble mediators of a Th1 response (IL-2 and IFN-γ) and Th2 response (IL-4) and proliferation of T lymphocytes sub-sets (CD4/CD8). Furthermore, these modified analogs of picroside-II were able to elicit a substantial increase in anti-OVA IgG when compared with OVA alone. These results support the use of acylated analogs particularly PK-II-3 and PK-II-4 as potent enhancer of antigen-specific Th1 and Th2 immune responses and thus are promising immune-adjuvant candidate for vaccines.
Kumar
H M Sampath
HM
Synthetic & Biological Chemistry Division, Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India. hmskumar@gmail.com
Singh
Parvinder Pal
PP
Qazi
Naveed A
NA
Srinivas
Jada
J
Malik
Fayaz
F
Sidiq
Tabasum
T
Gupta
Amit
A
Khajuria
Anamika
A
Suri
K A
KA
Satti
N K
NK
Qazi
G N
GN
D000215:Acylation; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D002934:Cinnamates / Q000008:administration & dosage* / Q000737:chemistry*; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D057888:Iridoid Glucosides / Q000008:administration & dosage* / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014611:Vaccination / Q000379:methods
D057134:Antibodies, Neutralizing; D002934:Cinnamates; D007074:Immunoglobulin G; D007376:Interleukin-2; D057888:Iridoid Glucosides; D015847:Interleukin-4; C416837:picroside II; D007371:Interferon-gamma; D010047:Ovalbumin