Proteomic analysis of diaminochlorotriazine (DACT) adducts in three brain regions of Wistar rats.
Atrazine (ATRA) is the most commonly applied herbicide in the United States and is detected frequently in drinking water at significant levels. Following oral exposure, metabolism of ATRA generates diaminochlorotriazine (DACT), an electrophilic molecule capable of forming covalent protein adducts. At high doses, both ATRA and DACT can disrupt the preovulatory luteinizing hormone (LH) surge in rats, thereby altering normal reproductive function. This research was designed to identify DACT protein adducts formed in three distinct brain regions of ATRA-exposed rats, including the preoptic area (POA), medial basal hypothalamus (MBH), and cortex (CTX). Proteins with DACT adducts were identified following 2-dimensional electrophoresis (2-DE), immunodetection, and MALDI-TOF mass spectrometry analysis. Western blots from exposed animals revealed over 30 DACT-modified spots that were absent in controls. Protein spots were matched to concurrently run 2-DE gels stained with Sypro Ruby, excised, and in-gel digested with trypsin.
Dooley
Gregory P
GP
The Center for Environmental Medicine, Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Ashley
Amanda K
AK
Legare
Marie E
ME
Handa
Robert J
RJ
Hanneman
William H
WH
D000284:Administration, Oral; D000818:Animals; D001280:Atrazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity; D007033:Hypothalamus, Middle / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D010449:Peptide Mapping; D011301:Preoptic Area / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006540:Herbicides; D011506:Proteins; C526766:diaminochlorotriazine; D001280:Atrazine