Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium.
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 degrees C and 37 degrees C, reaching a maximum production of 1.0 x 10(9) AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity.
Todorov
Svetoslav Dimitrov
SD
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Laboratório de Microbiologia de Alimentos, Av. Prof. Lineu Prestes 580, Bloco 14, 05508-000 São Paulo-SP, Brazil.
Wachsman
Monica
M
Tomé
Elisabetta
E
Dousset
Xavier
X
Destro
Maria Teresa
MT
Dicks
Leon Milner Theodore
LM
Franco
Bernadette Dora Gombossy de Melo
BD
Vaz-Velho
Manuella
M
Drider
Djamel
D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D000998:Antiviral Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D016984:Enterococcus faecium / Q000378:metabolism / Q000502:physiology*; D005516:Food Microbiology; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008087:Listeria / Q000187:drug effects; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D012474:Salmon / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D001430:Bacteriocins