Structure of the O-polysaccharide of Cronobacter sakazakii O2 with a randomly O-acetylated l-rhamnose residue.
Studies by sugar analysis and partial acid hydrolysis along with one- and two-dimensional (1)H and (13)C NMR spectroscopy and high-resolution ESI MS showed that the O-polysaccharide (O-antigen) Cronobacter sakazakii ATCC 29004 (serotype O2) possesses a branched hexasaccharide O-unit with a randomly mono-O-acetylated terminal rhamnose residue in the side chain and the following structure: [Formula: see text]. A similar structure has been reported for the O-polysaccharide of C. sakazakii 767, which differs in the presence of an additional lateral alpha-d-Glcp residue on GlcNAc and the pattern of O-acetylation (Czerwicka, M., Forsythe, S. J.; Bychowska, A.; Dziadziuszko, H.; Kunikowska, D.; Stepnowski, P.; Kaczynski, Z. Carbohydr. Res.2010, 345, 908-913).
Arbatsky
Nikolay P
NP
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Wang
Min
M
Shashkov
Alexander S
AS
Chizhov
Alexander O
AO
Feng
Lu
L
Knirel
Yuriy A
YA
Wang
Lei
L
D000107:Acetylation; D002240:Carbohydrate Sequence; D044083:Cronobacter sakazakii; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D012210:Rhamnose; D021241:Spectrometry, Mass, Electrospray Ionization
D019081:O Antigens; D012210:Rhamnose