title: Multi-walled carbon nanotube (MWCNT) synthesis, preparation, labeling, and functionalization.
abstract: Nanomedicine is a growing field with a great potential for introducing new generation of targeted and personalized drug. Amongst new generation of nano-vectors are carbon nanotubes (CNTs), which can be produced as single or multi-walled. Multi-walled carbon nanotubes (MWCNTs) can be fabricated as biocompatible nanostructures (cylindrical bulky tubes). These structures are currently under investigation for their application in nanomedicine as viable and safe nanovectors for gene and drug delivery. In this chapter, we will provide you with the necessary information to understand the synthesis of MWCNTs, functionalization, PKH26 labeling, RNAi, and DNA loading for in vitro experimentation and in vivo implantation of labeled MWCNT in mice as well as materials used in this experimentation. We used this technique to manipulate microglia as part of a novel application for the brain cancer immunotherapy. Our published data show this is a promising technique for labeling, and gene and drug delivery into microglia.
authors.affiliation: Brain Mapping Foundation and International Brain Mapping & Intraoperative Surgical Planning Society (IBMISPS), West Hollywood, CA, USA.
authors.forename: Babak
authors.initials: B
authors.lastname: Kateb
authors.forename: Vicky
authors.initials: V
authors.lastname: Yamamoto
authors.forename: Darya
authors.initials: D
authors.lastname: Alizadeh
authors.forename: Leying
authors.initials: L
authors.lastname: Zhang
authors.forename: Harish M
authors.initials: HM
authors.lastname: Manohara
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bronikowski
authors.forename: Behnam
authors.initials: B
authors.lastname: Badie
mesh_terms: D000818:Animals; D002460:Cell Line; D020007:Implants, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D037742:Nanotubes, Carbon; D034741:RNA, Small Interfering