Steamed ginseng-leaf components enhance cytotoxic effects on human leukemia HL-60 cells.
Three new dammarane-type glycosides, named ginsenosides SL(1)-SL(3) (1-3), and eleven known compounds (4-14) were isolated from the heat-processed leaves of Panax ginseng. Their structures were elucidated on the basis of extensive chemical and spectroscopic methods. Cytotoxic-activity testing of compounds 1-14 against human leukemia HL-60 cells showed that ginsenosides Rh(3) (11) and Rk(2) (12) exhibited potent effects with IC(50) values of 0.8 and 0.9 microM. In addition, ginsenosides SL(3) (3), 20S-Rg(2) (7), F(4) (10), 20S-Rh(2) (13) displayed strong activity with IC(50) values of 9.0, 9.0, 7.5, and 8.2 microM, respectively. This is the first report on chemical components of the steamed ginseng leaves.
Tung
Nguyen Huu
NH
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Song
Gyu Yong
GY
Minh
Chau Van
CV
Kiem
Phan Van
PV
Jin
Long Guo
LG
Boo
Hye-Jin
HJ
Kang
Hee-Kyoung
HK
Kim
Young Ho
YH
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008968:Molecular Conformation; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D013237:Stereoisomerism
D000970:Antineoplastic Agents; D036145:Ginsenosides; D010936:Plant Extracts; C554027:ginsenoside SL1; C554030:ginsenoside SL2; C554029:ginsenoside SL3