Application of an active alginate coating to control the growth of Listeria monocytogenes on poached and deli turkey products.
The relatively high prevalence of Listeria monocytogenes in ready-to-eat (RTE) turkey products is of great concern. The overall objective of this study was to develop antimicrobial edible coating formulations to effectively control the growth of this pathogen. The antimicrobials studied were nisin (500IU/g), Novagard CB 1 (0.25%), Guardian NR100 (500ppm), sodium lactate (SL, 2.4%), sodium diacetate (SD, 0.25%), and potassium sorbate (PS, 0.3%). These were incorporated alone or in binary combinations into five edible coatings: alginate, kappa-carrageenan, pectin, xanthan gum, and starch. The coatings were applied onto the surface of home-style poached and processed deli turkey discs inoculated with ~3log CFU/g of L. monocytogenes. The turkey samples were then stored at 22 degrees C for 7days. For poached and processed deli turkey, the coatings were found to be equally effective, with pectin being slightly less effective than the others. The most effective poached turkey treatments seemed to be SL (2.4%)/SD (0.25%) and Nisin (500IU/g)/SL (2.4%), which yielded final populations of 3.0 and 4.9log CFU/g respectively compared to the control which was 7.9log CFU/g. For processed deli turkey, the most effective antimicrobial treatments seemed to be Nisin (500IU/g)/SD (0.25%) and Nisin (500IU/g)/SL (2.4%) with final populations of 1.5 and 1.7log CFU/g respectively compared to the control which was 6.5log CFU/g. In the second phase of the study, home-style poached and store-purchased roasted (deli) turkey inoculated with the pathogen at a level of ~3log CFU/g were coated with alginate incorporating selected antimicrobial combinations and stored for 8weeks at 4 degrees C. Alginate coatings supplemented with SL (2.4%)/PS (0.3%) delayed the growth of L. monocytogenes with final counts reaching 4.3log CFU/g (home-style poached turkey) and 6.5log CFU/g (roasted deli turkey) respectively while the counts in their untreated counterparts were significantly higher (P<0.05) reaching 9.9 and 7.9log CFU/g, respectively. This study therefore demonstrates the effectiveness of using alginate-based antimicrobial coatings to enhance the microbiological safety and quality of RTE poultry products during chilled storage.
Juck
Greg
G
Neetoo
Hudaa
H
Chen
Haiqiang
H
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003080:Cold Temperature; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008461:Meat Products / Q000382:microbiology*; D013997:Time Factors; D014422:Turkeys
D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D020723:Glucuronic Acid