[Actins and keratins in the diagnosis of human basal-like breast cancer].
The most common forms of luminal breast cancer (BC) were compared with basal-like and Her2/neu3+ BC. Their primary classification was based on morphological diagnosis and the expression of estrogen receptor (ER), progesterone receptor (PR), and Her2/neu receptors. Monoclonal antibodies to actins and keratins were used for the study. Basal-like BC cells (ER/PR/Her2/ neu-) were regularly stained with antibodies to basal keratins 5/6 and 17, smooth muscle alpha-actin, and p63. Luminal keratin 8 staining was reduced. The solid regions had beta-actin staining with disappearance in the scirrhous component. beta-actin and basal keratins were also observed in metaplastic BC with ER/PR/Her2/neu3+. Immunomorphology using cytoskeletal markers along with the expression of steroid hormone and Her2/neu receptors may be used in the diagnosis of basal-like forms of BC.
Dugina
V B
VB
Ermilova
V D
VD
Chemeris
G Iu
GIu
Chipysheva
T A
TA
D000199:Actins / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007633:Keratins / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D018295:Neoplasms, Basal Cell / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies
D000199:Actins; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D007633:Keratins