M-marker 2009, a marker set for mapping medaka mutants using PCR length polymorphisms with an automated microchip gel electrophoresis system.
We have developed a highly effective marker set for assigning medaka mutants to specific linkage groups (LG) in bulk segregation analysis by PCR product length polymorphisms (PLPs). The marker set permits analysis using an automated microchip electrophoresis system as well as conventional agarose gel electrophoresis.
Kimura
Tetsuaki
T
National Institute for Basic Biology, Laboratory of Bioresources, The Graduate University for Advanced Studies (SOKRNDAI), Okazaki, Japan. tekimura@nibb.ac.jp
Naruse
Kiyoshi
K
D000818:Animals; D001331:Automation; D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D005819:Genetic Markers; D009154:Mutation / Q000235:genetics*; D009990:Oryzias / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*
D005819:Genetic Markers