DNA barcoding techniques for avian influenza virus surveillance in migratory bird habitats.
Avian influenza virus (AIV) circulates among free-ranging, wild birds. We optimized and validated a DNA barcoding technique for AIV isolation and host-species identification using fecal samples from wild birds. DNA barcoding was optimized using tissue and fecal samples from known bird species, and the method was shown to distinguish 26 bird species. Subsequently, fecal samples (n=743) collected from wild waterfowl habitats confirmed the findings from the laboratory tests. All identified AIV-positive hosts (n=35) were members of the order Anseriformes. We successfully applied the DNA barcoding technique to AIV surveillance and examined AIV epidemiology and host ecology in these wild waterfowl populations. This methodology may be useful in the design of AIV surveillance strategies.
Lee
Dong-Hun
DH
Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
Lee
Hyun-Jeong
HJ
Lee
Youn-Jeong
YJ
Kang
Hyun-Mi
HM
Jeong
Ok-Mi
OM
Kim
Min-Chul
MC
Kwon
Ji-Sun
JS
Kwon
Jun-Hun
JH
Kim
Chang-Bae
CB
Lee
Joong-Bok
JB
Park
Seung-Yong
SY
Choi
In-Soo
IS
Song
Chang-Seon
CS
D025041:Animal Migration; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D046090:Anseriformes / Q000821:virology*; D001717:Birds; D004279:DNA, Viral / Q000032:analysis*; D017753:Ecosystem; D005243:Feces / Q000821:virology; D005260:Female; D009980:Influenza A virus / Q000235:genetics*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000635:transmission; D007723:Korea / Q000453:epidemiology; D008297:Male; D018571:Sentinel Surveillance / Q000662:veterinary*; D017422:Sequence Analysis, DNA
D004279:DNA, Viral