title: DNA barcoding techniques for avian influenza virus surveillance in migratory bird habitats.
abstract: Avian influenza virus (AIV) circulates among free-ranging, wild birds. We optimized and validated a DNA barcoding technique for AIV isolation and host-species identification using fecal samples from wild birds. DNA barcoding was optimized using tissue and fecal samples from known bird species, and the method was shown to distinguish 26 bird species. Subsequently, fecal samples (n=743) collected from wild waterfowl habitats confirmed the findings from the laboratory tests. All identified AIV-positive hosts (n=35) were members of the order Anseriformes. We successfully applied the DNA barcoding technique to AIV surveillance and examined AIV epidemiology and host ecology in these wild waterfowl populations. This methodology may be useful in the design of AIV surveillance strategies.
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Youn-Jeong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Kang
authors.forename: Ok-Mi
authors.initials: OM
authors.lastname: Jeong
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Kwon
authors.forename: Jun-Hun
authors.initials: JH
authors.lastname: Kwon
authors.forename: Chang-Bae
authors.initials: CB
authors.lastname: Kim
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
mesh_terms: D025041:Animal Migration; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D046090:Anseriformes / Q000821:virology*; D001717:Birds; D004279:DNA, Viral / Q000032:analysis*; D017753:Ecosystem; D005243:Feces / Q000821:virology; D005260:Female; D009980:Influenza A virus / Q000235:genetics*; D005585:Influenza in Birds / Q000453:epidemiology* / Q000635:transmission; D007723:Korea / Q000453:epidemiology; D008297:Male; D018571:Sentinel Surveillance / Q000662:veterinary*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral