Isolation and identification of polymethoxyflavones from the hybrid Citrus, hallabong.
Seven polymethoxyflavones (PMFs) were isolated from the dried peels of hallabong, the hybrid Citrus , by a repeated column chromatography. The structures of PMFs were identified as 5,6,7,3',4'-pentamethoxyflavone (1), 6,7,8,3',4'-pentamethoxyflavone (2), 3-hydroxy-5,6,7,4'-tetramethoxyflavone (3), 5,6,7,8,3',4'-hexamethoxyflavone (4), 3,6,7,4'-tetramethoxyflavone (5), 3,5,6,7,8,3',4'-heptamethoxyflavone (6), and 5,6,7,8,4'-pentamethoxyflavone (7) using 1H and 13C NMR in combination with mass spectrometry. Among these compounds, 5 was isolated for the first time from nature. The content of PMFs 1-7 in hallabong was determined by HPLC-UV. The major PMFs of hallabong are 5 in the dried peels (15.4 mg/g) and 7 in the dried leaves (12.2 mg/g).
Han
Saem
S
Department of Applied Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea.
Kim
Hye Min
HM
Lee
Jeong Min
JM
Mok
So-Youn
SY
Lee
Sanghyun
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D047309:Flavones