Analysis of the function of cytoplasmic fibers formed by the rubella virus nonstructural replicase proteins.
The P150 and P90 replicase proteins of rubella virus (RUBV), a plus-strand RNA Togavirus, produce a unique cytoplasmic fiber network resembling microtubules. Pharmacological and mutagenic approaches were used to determine if these fibers functioned in virus replication. The pharmacological approach revealed that microtubules were required for fiber formation, but neither was necessary for virus replication. Through the mutagenic approach it was found that α-helices near both termini of P150 were necessary for fiber assembly and infectivity, but fiber formation and viability could not be correlated because most of these mutations were lethal. The N-terminal α-helix of P150 affected both proteolytic processing of P150 and P90 from the P200 precursor and targeting of P200, possibly through directing conformational folding of P200. Finally, we made the unexpected discovery that RUBV genomes can spread from cell-to-cell without virus particles, a process that we hypothesize utilizes RUBV-induced cytoplasmic projections containing fibers and replication complexes.
Matthews
Jason D
JD
Georgia State University, Department of Biology, Atlanta, GA 30303, USA.
Tzeng
Wen-Pin
WP
Frey
Teryl K
TK
D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism* / Q000821:virology; D006801:Humans; D022101:Microtubule-Organizing Center / Q000378:metabolism / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012409:Rubella / Q000378:metabolism* / Q000821:virology; D012412:Rubella virus / Q000235:genetics / Q000502:physiology*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D017361:Viral Nonstructural Proteins