Single-cell detection using a thin film transistor photosensor with micro-partitions.
A thin film transistor (TFT) photosensor was applied to single-cell detection by identifying cell surface molecules based on chemiluminescence. Micro-partitions were directly fabricated on the TFT photosensor surface by photolithography. The surface of each pixel was surrounded by 25 μm-height partitions, forming areas of approximately 30 μm × 30 μm for cell entrapment and photosensing. Visualization of individual JM cells, stained with mouse anti-human CD8 IgG1 primary antibody and Horseradish peroxidase (HRP)-labeled anti-mouse IgG1 secondary antibody, as bright-pixels was successfully achieved using the micro-partitioned TFT photosensor integrated into a microfluidic chamber. Furthermore, real-time monitoring of HRP-labeled JM cells was also accomplished. The fabrication of micro-partitions on the surface of the TFT photosensor allows highly efficient single-cell entrapment and chemiluminescence-based detection of JM cells. This is the first report of single-cell entrapment and subsequent signal detection on the photosensing area of individual pixels of TFT photosensor. This system will allow high-throughput and real-time analysis of more than 10(4) cells with minimum optical system requirements.
Tanaka
Tsuyoshi
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
Sunaga
Yoshihiko
Y
Hatakeyama
Keiichi
K
Matsunaga
Tadashi
T
D000818:Animals; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry; D016827:CD8 Antigens / Q000737:chemistry; D002460:Cell Line; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008027:Light; D051379:Mice; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010316:Particle Size; D010777:Photochemistry / Q000379:methods; D011137:Polystyrenes / Q000737:chemistry
D016827:CD8 Antigens; D007074:Immunoglobulin G; D011137:Polystyrenes; D001710:Biotin; D006735:Horseradish Peroxidase