[Initial analysis of the results of the genome-wide search for the genes responsive to interferon alpha].
The paper is focused on the primary analysis of the potential genes of primary response to IFNalpha, predicted by the program COTRASIF elaborated in our laboratory. Two web-instruments FatiGO and GOTM were applied for this purpose; the literature search was carried out using IHOP; genes were classified according to the conceptual diagram "Gene Ontology" (GO) and were ranked according to their first priority for the experimental validation. On the basis of conducted analysis 162 genes of potential primary response to IFNalpha were subdivided into two groups--61 genes, for which the experimental data of their responsibility to IFNalpha were found and 101 genes for which such data are absent. We have performed the functional analysis of the "unknown" genes according to GO. The enriched category "Synapse part" encountering three genes of Rattus norvegicus: PRKCA-binding of protein, NMDAR-L and GABAA--receptor subunit alpha-2 were revealed. Among 162 genes the enriched category "Immune response" was detected with Mbl1 gene, which is reckoned among "unknown" genes. The four designated genes are chosen as the candidates for the prior validation.
Drahushchenko
O O
OO
Tokovenko
B T
BT
Obolens'ka
M Iu
MIu
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D019295:Computational Biology; D003598:Cytoskeletal Proteins; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D016678:Genome; D016898:Interferon-alpha / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D051381:Rats / Q000235:genetics*; D011963:Receptors, GABA-A / Q000235:genetics
D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C520011:Gabra2 protein, rat; C096392:Grin3a protein, rat; D016898:Interferon-alpha; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; C498298:PICK1 protein, rat; D011963:Receptors, GABA-A