title: Application of leukocyte antisedimentation rate calculation in investigation of spleen salvaging experimental surgical techniques.
abstract: Clinical studied showed leukocyte antisedimentation rate (LAR) changes in various conditions that modulate the immune system. There is a lack of LAR data in animal experiments concerning splenic function after splenectomy or spleen preserving operations, thus we aimed to include LAR in our ongoing inbred canine study on following-up spleen salvaging surgical techniques. Blood samples of healthy beagle dogs served as normal control. The experimental groups were: "SH"-sham operated, "SE"-splenectomy, "R1/3" and "R2/3"-one-third/two-third of the spleen resected, "AU-5" and "AU-10"-autotransplantation with 5/10 spleen chips using Furka's techniques. On the 12th postoperative month Rabigen-Mono+Vanguard-Plus-5 vaccines were given to the animals, which provoked immune response. Blood samples were taken before and one week after the vaccination. LAR was calculated by Bogar's method. In normal control group LAR was 3.11, leukocyte count was 12.04 G/l. In the experimental groups LAR varied between 2.4-7.3 before vaccination. After vaccination LAR increased in all groups. Although "SE" group expressed the largest changes (LAR = 16.56; 135%), the leukocyte count increased only by 12%. In "AU" groups the increase was smaller, while "R" groups showed slight changes only. LAR can be applicable in animal experiments and may serve as a supplementary parameter in hemorheological investigations of hyposplenic-asplenic states.
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. sajtose@med.unideb.hu
authors.forename: Erika
authors.initials: E
authors.lastname: Sajtos
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kiss
authors.forename: Endre
authors.initials: E
authors.lastname: Brath
authors.forename: Katalin
authors.initials: K
authors.lastname: Peto
authors.forename: Timea
authors.initials: T
authors.lastname: Hever
authors.forename: Lili
authors.initials: L
authors.lastname: Matyas
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
mesh_terms: D000818:Animals; D004285:Dogs; D007109:Immunity; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D013154:Spleen / Q000276:immunology* / Q000601:surgery; D013156:Splenectomy; D014611:Vaccination