Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis.
A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in shoot tissue than in inflorescences. Transgenic Arabidopsis plants expressing antisense DlEMF2 displayed a spectrum of early-flowering phenotypes. Quantitative real-time PCR experiments indicated that the antisense transgene DlEMF2 reduced endogenous AtEMF2 transcript levels leading to the flowering time reduction. Besides, transforming the bamboo DlEMF2 gene into Arabidopsis emf2-1 mutant could rescue phenotype in vegetative and reproductive developmental stages, which reveals that EMF2 has functional conservation between monocots and eudicots.
Xu
Hong
H
Plant Germplasm and Genomics Centre, Germplasm Bank of Wild Species, Kunming, 650204, Yunnan, People's Republic of China.
Chen
Ling-Jing
LJ
Qu
Li-Jia
LJ
Gu
Hong-Ya
HY
Li
De-Zhu
DZ
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D017124:Conserved Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D020868:Gene Silencing; D008969:Molecular Sequence Data; D006109:Poaceae / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C494299:EMF2 protein, Arabidopsis; D016372:RNA, Antisense; D012097:Repressor Proteins