Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge.
Eimeria acervulina 3-1E antigen gene and mature chicken interleukin 15 (mChIL-15) gene were cloned into expression vector pcDNA3.1(+) in different forms, produced DNA vaccine pcDNA3.1-3-1E, and pcDNA3.1-3-1E-linker-mChIL-15 co-expressing E. acervulina 3-1E gene and mChIL-15 gene, respectively. The expression of objective gene in vitro was detected by indirect fluorescent antibody technique and immunohistochemistry. The two DNA vaccines were administered by intramuscular leg injection. An animal challenge experiment was carried out to evaluate the immune protective efficacy of the vaccines. The results indicated that DNA vaccines were successfully constructed and the expression of objective gene could be detected in vitro. The animal experimental results showed that both DNA vaccines could provide partial protection against homologous challenge in chickens. The chimeric DNA vaccine, pcDNA3.1-3-1E-linker-mChIL-15, could significantly increase oocyst decrease ratio, reduce the average lesion score in the duodenum, improve body weight gain, and increase anti-coccidial index (ACI) compared to the DNA vaccine pcDNA3.1-3-1E. Taken together, these results demonstrate ChIL-15 enhance the immunogenicity of 3-1E DNA vaccine, and co-expression of cytokine and optimized surface antigen of Eimeria may be a promising method to enhance immunogenicity of DNA vaccines in poultry.
Ma
Dexing
D
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Ma
Chunli
C
Pan
Long
L
Li
Guangxing
G
Yang
Jinghong
J
Hong
Jiehua
J
Cai
Haofan
H
Ren
Xiaofeng
X
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D002645:Chickens / Q000469:parasitology*; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D019409:Interleukin-15 / Q000235:genetics / Q000276:immunology*; D010957:Plasmids; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D016052:Protozoan Vaccines; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D019444:Vaccines, DNA
D000953:Antigens, Protozoan; D019409:Interleukin-15; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA