Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress.
The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2',7'-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.
Kang
Kyoung Ah
KA
School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, South Korea.
Lee
In Kyung
IK
Zhang
Rui
R
Piao
Mei Jing
MJ
Kim
Ki Cheon
KC
Kim
Sang Young
SY
Shin
Taekyun
T
Kim
Bum Joon
BJ
Lee
Nam Ho
NH
Hyun
Jin Won
JW
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011506:Proteins / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D005960:Glucosides; D047348:Hydrolyzable Tannins; D011506:Proteins; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C024603:Geraniin