A highly selective low-background fluorescent imaging agent for nitric oxide.
We introduce a novel sensing mechanism for nitric oxide (NO) detection with a particular easily synthesized embodiment (NO(550)), which displays a rapid and linear response to NO with a red-shifted 1500-fold turn-on signal from a dark background. Excellent selectivity was observed against other reactive oxygen/nitrogen species, pH, and various substances that interfere with existing probes. NO(550) crosses cell membranes but not nuclear membranes and is suitable for both intra- and extracellular NO quantifications. Good cytocompatibility was found during in vitro studies with two different cell lines. The high specificity, dark background, facile synthesis, and low pH dependence make NO(550) a superior probe for NO detection when used as an imaging agent.
Yang
Youjun
Y
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
Seidlits
Stephanie K
SK
Adams
Michelle M
MM
Lynch
Vincent M
VM
Schmidt
Christine E
CE
Anslyn
Eric V
EV
Shear
Jason B
JB
D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009569:Nitric Oxide / Q000737:chemistry*; D012995:Solubility
D005456:Fluorescent Dyes; D009569:Nitric Oxide