title: [Effect of Mortierlla isabellina delta5-desaturases gene on arachidonic acid synthesis].
abstract: OBJECTIVE
  To study the relation between mRNA expression of delta5-desaturase gene and arachidonic acid production.
  
  
  METHODS
  Using fluorescence quantitative PCR, we determined the mRNA expression levels of delta5-desaturase gene of five Mortierlla isabellina strains cultured for the same time and strain YZ-124 cultured for different time. Simultaneously, we measured the contents of arachidonic acid by gas chromatography.
  
  
  RESULTS
  The results showed that delta5-desaturase gene mRNA expression level of distinct strains was different. Expression level of the original strain As3. 3410 was the lowest, whereas that of the mutant strain YZ-124 was the highest. The mRNA expression of delta5-desaturases gene decreased gradually with fermentation time.
  
  
  CONCLUSIONS
  According to arachidonic acid yield, mRNA expression quantity of delta5-desaturase gene presented a positive correlation with arachidonic acid content in cultured oil.
authors.affiliation: College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China. spxypjb@126.com
authors.forename: Changqing
authors.initials: C
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Di
authors.initials: D
authors.lastname: Yao
authors.forename: Lina
authors.initials: L
authors.lastname: Li
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D016718:Arachidonic Acid; D044943:Fatty Acid Desaturases