title: Sanguinarine induces apoptosis of human osteosarcoma cells through the extrinsic and intrinsic pathways.
abstract: The quaternary benzo[c]phenanthridine alkaloid sanguinarine inhibits the proliferation of cancerous cells from different origins, including lung, breast, pancreatic and colon, but nothing is known of its effects on osteosarcoma, a primary malignant bone tumour. We have found that sanguinarine alters the morphology and reduces the viability of MG-63 and SaOS-2 human osteosarcoma cell lines in concentration- and time-dependent manner. Incubation with 1 micromol/L sanguinarine for 4 and 24h killed more efficiently MG-63 cells than SaOS-2 cells, while incubation with 5 micromol/L sanguinarine killed almost 100% of both cell populations within 24h. This treatment also changed the mitochondrial membrane potential in both MG-63 and SaOS-2 cells within 1h, caused chromatin condensation and the formation of apoptotic bodies. It activated multicaspases, and increased the activities of caspase-8 and caspase-9 in both MG-63 and SaOS-2 cells. These data highlight sanguinarine as a novel potential agent for bone cancer therapy.
authors.affiliation: Cell-Biomaterial Biohybrid Systems, Université de Sherbrooke, Department of Chemical Engineering and Biotechnological Engineering, Canada J1K 2R1.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Park
authors.forename: Eric
authors.initials: E
authors.lastname: Bergeron
authors.forename: Helena
authors.initials: H
authors.lastname: Senta
authors.forename: Kim
authors.initials: K
authors.lastname: Guillemette
authors.forename: Sabrina
authors.initials: S
authors.lastname: Beauvais
authors.forename: Richard
authors.initials: R
authors.lastname: Blouin
authors.forename: Joël
authors.initials: J
authors.lastname: Sirois
authors.forename: Nathalie
authors.initials: N
authors.lastname: Faucheux
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D053119:Benzophenanthridines / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D007546:Isoquinolines; C005705:sanguinarine; D053181:Caspase 8; D053453:Caspase 9