Effects of diallyl sulphide in chondrocyte and cartilage in experimental osteoarthritis in rabbit.
Diallyl sulphide (DAS) is known for its antioxidant, anticancer and detoxifying properties. The aim of this study was to investigate the effect of DAS on rabbit articular chondrocytes and cartilage in experimental osteoarthritis (OA) induced by anterior cruciate ligament transection (ACLT). DAS inhibited matrix metalloproteinase-1 (MMP-1), MMP-3 and MMP-13 expression in interleukin-1beta (IL-1β)-induced chondrocytes. In an in vivo study, DAS ameliorated cartilage degradation as assessed by morphological and histological examination. Messenger RNA expression of MMP-1, MMP-3, MMP-13 and IL-1β was inhibited by DAS in cartilage. In addition, DAS increased the collagen II level in cartilage. The results suggest that DAS may protect cartilage in the development of OA.
Chen
Wei-Ping
WP
Department of Orthopedics Surgery, The Second Hospital of Medical College, Zhejiang University, Hangzhou, PR China.
Tang
Jing-Li
JL
Bao
Jia-Peng
JP
Hu
Peng-Fei
PF
Yu
Chong
C
Shi
Zhong-Li
ZL
Wu
Li-Dong
LD
D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D024043:Collagen Type II; D053583:Interleukin-1beta; D012333:RNA, Messenger; D013440:Sulfides; C038491:allyl sulfide; D053509:Matrix Metalloproteinase 13; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1