Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea.
The full-length sequences of Botrytiscinereamitovirus 1 (BcMV1) and an associated RNA (BcMV1-S) in strain CanBc-1c-78 of Botrytis cinerea were determined. Sequence analysis showed that BcMV1 is 2804 nt long and AU-rich (66.8%). BcMV1 shares 95% nucleotide sequence identity with Ophiostomanovo-ulmimitovirus 3b (OnuMV3b). However, it is 472 nt longer than OnuMV3b. Mitochondrial codon usage revealed that BcMV1 contains one open reading frame encoding RdRp, which is 96% identical to the RdRp of OnuMV3b. These findings confirm that BcMV1 belongs to the genus Mitovirus and is a strain of OnuMV3b. BcMV1-S is 2171 nt long and derived from BcMV1 through a single internal in-frame deletion of 633 nt, suggesting that it is a defective RNA of BcMV1. BcMV1-S was found to suppress the replication of BcMV1 and to be co-transmissible with BcMV1 through hyphal anastomosis. Its presence, however, did not alleviate the BcMV1-associated debilitation phenotypes of B. cinerea.
Wu
Mingde
M
The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Zhang
Lei
L
Li
Guoqing
G
Jiang
Daohong
D
Ghabrial
Said A
SA
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D020171:Botrytis / Q000472:pathogenicity / Q000648:ultrastructure / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D055156:Ophiostoma / Q000821:virology; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase