Eicosanoids in the innate immune response: TLR and non-TLR routes.
The variable array of pattern receptor expression in different cells of the innate immune system explains the induction of distinct patterns of arachidonic acid (AA) metabolism. Peptidoglycan and mannan were strong stimuli in neutrophils, whereas the fungal extract zymosan was the most potent stimulus in monocyte-derived dendritic cells since it induced the production of PGE(2), PGD(2), and several cytokines including a robust IL-10 response. Zymosan activated kappaB-binding activity, but inhibition of NF-kappaB was associated with enhanced IL-10 production. In contrast, treatments acting on CREB (CRE binding protein), including PGE(2), showed a direct correlation between CREB activation and IL-10 production. Therefore, in dendritic cells zymosan induces il10 transcription by a CRE-dependent mechanism that involves autocrine secretion of PGE(2), thus unraveling a functional cooperation between eicosanoid production and cytokine production.
Alvarez
Yolanda
Y
Instituto de Biología y Genética Molecular, Valladolid, Spain.
Valera
Isela
I
Municio
Cristina
C
Hugo
Etzel
E
Padrón
Francisco
F
Blanco
Lydia
L
Rodríguez
Mario
M
Fernández
Nieves
N
Crespo
Mariano Sánchez
MS
D001483:Base Sequence; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D015777:Eicosanoids / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000276:immunology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology*; D015054:Zymosan / Q000276:immunology
D017362:Cyclic AMP Response Element-Binding Protein; D015777:Eicosanoids; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D016328:NF-kappa B; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D051193:Toll-Like Receptors; D016753:Interleukin-10; D015054:Zymosan; D051546:Cyclooxygenase 2