title: Identification of a tumour associated antigen in lung cancer patients with asbestos exposure.
abstract: BACKGROUND
  This study analysed the humoral immune response in asbestos exposed lung cancer patients to identify new surrogate markers of the carcinogenic risk in populations exposed to asbestos.
  
  
  RESULTS
  A serological analysis identified five distinct antigens reactive with IgG derived from a lung cancer patient with high asbestos exposure. In one of the isolated antigens, quantitative RT-PCR indicated that annexin A2 (AnxA2) was overexpressed in lung cancer tissues and normal lung from patients with high asbestos exposure. Antibody against AnxA2 was detected in 9/15 (60%) of lung cancer patients with high asbestos exposure; however, in only 1/12 (8%) of lung cancer patients with low asbestos exposure. AnxA2 was also overexpressed in malignant mesothelioma cells, and the antibody was also positive in 8/15 (53%) of patients with malignant mesothelioma.
  
  
  CONCLUSIONS
  The antibody titer against AnxA2 may be a potentially useful new diagnostic surrogate marker for asbestos-related lung cancer and malignant mesothelioma.
authors.affiliation: Second Department of Surgery, University of Occupational and Environmental Health, Japan, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555 Japan. m-yasuda@med.uoeh-u.ac.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Yasuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hanagiri
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Shigematsu
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Onitsuka
authors.forename: Kouji
authors.initials: K
authors.lastname: Kuroda
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Baba
authors.forename: Makiko
authors.initials: M
authors.lastname: Mizukami
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ichiki
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Uramoto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takenoyama
authors.forename: Kosei
authors.initials: K
authors.lastname: Yasumoto
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017306:Annexin A2 / Q000096:biosynthesis / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001194:Asbestos / Q000276:immunology / Q000506:poisoning*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D008175:Lung Neoplasms / Q000209:etiology* / Q000276:immunology*; D008297:Male; D008654:Mesothelioma / Q000209:etiology / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G; D015850:Interleukin-6; D001194:Asbestos