Colorectal cancer and genetic polymorphism of DNA double-strand break repair gene XRCC4 in Taiwan.
The DNA repair gene X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4) is thought to play a major role in the caretaking of the whole genome via double-strand break repair. However, the association of polymorphic variants of XRCC4 with colorectal cancer susceptibility has never been reported. In this hospital-based case-control study, the association of XRCC4 polymorphisms C-1622T (rs7727691), G-1394T (rs6869366), G-652T (rs2075685), C-571T (rs2075686), intron 3 DIP (rs28360071), S247A (rs3734091) and intron 7 DIP (rs28360317) with colorectal cancer risk in a Taiwanese population was investigated. The genotypes of XRCC4 of 370 patients with colorectal cancer and 370 age- and gender-matched healthy controls were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. We found significant differences in the genetic and allelic frequencies of the XRCC4 G-1394T between the colorectal cancer and control groups (p=0.0003 and 8.32 x 10(-5), respectively). The distributions of other genetic polymorphisms between cases and the control group were not significantly different. We conclude that the G allele of XRCC4 G-1394T may contribute to colorectal carcinogenesis and may be useful for early detection of colorectal cancer.
Bau
Da-Tian
DT
Terry Fox Cancer Research Lab, China Medical University Hospital, 2 Yuh-Der Road, Taichung, 404 Taiwan, R.O.C. datian@mail.cmuh.org.tw
Yang
Mei-Due
MD
Tsou
Yung-An
YA
Lin
Song-Shei
SS
Wu
Cheng-Nan
CN
Hsieh
Hao-Hsueh
HH
Wang
Rou-Fen
RF
Tsai
Chia-Wen
CW
Chang
Wen-Shin
WS
Hsieh
Hsiu-Min
HM
Sun
Shung-Shung
SS
Tsai
Ru-Yin
RY
D000483:Alleles; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007438:Introns; D011110:Polymorphism, Genetic; D013624:Taiwan
D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human