title: Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.
abstract: BACKGROUND
  Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear.
  
  
  RESULTS
  We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos.
  
  
  CONCLUSIONS
  Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyonic tissues.
authors.affiliation: Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Ediriweera P S
authors.initials: EP
authors.lastname: Chandana
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Maeda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ueda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.forename: Naoko
authors.initials: N
authors.lastname: Oshima
authors.forename: Mako
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Shunya
authors.initials: S
authors.lastname: Kondo
authors.forename: Junseo
authors.initials: J
authors.lastname: Oh
authors.forename: Rei
authors.initials: R
authors.lastname: Takahashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawashima
authors.forename: David B
authors.initials: DB
authors.lastname: Alexander
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kitayama
authors.forename: Chiaki
authors.initials: C
authors.lastname: Takahashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Noda
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000098:blood supply*; D005260:Female; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D018919:Neovascularization, Physiologic; D011247:Pregnancy; D014599:Uterus / Q000098:blood supply*
chemical_list: D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C492025:Reck protein, mouse