[Cloning of amplicon of chromosomal SCO1206 sequences of Streptomyces coelicolor A 3(2)].
With the help of polymerase cycle reaction a fragment of chromosome Streptomyces coelicolor A 3(2) was amplified which contains gene SCO1206 coding subunit proteins of polyketide synthase III. Cloning of amplified DNA in the structure of shuttle vectors pAX5a and pWHM4 in Escherichia coli XL1 Blue was done. Plasmids of some selected ampicillin-resistent clones were transformed into protoplasts of plasmidless variant of Streptomyces globisporus 1912. Restrictional analysis of plasmid DNA of all investigated transformants both Escherichia coli and Streptomyces globisporus 1912 showed that 1.1 kb fragments of DNA were cloned in them. Research of the complex of metabolites, synthesized by transformants of Streptomyces globisporus 1912 revealed the presence of new substances which were not synthesized by the recipient.
Luk'ianchuk
V V
VV
Polishchuk
L V
LV
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D048630:Polyketide Synthases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D021122:Protein Subunits; D011523:Protoplasts; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D021122:Protein Subunits; D048630:Polyketide Synthases; D004262:DNA Restriction Enzymes