Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids.
Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxycurcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a=72.7, b=97.2, c=126.2 A, alpha=gamma=90.0, beta=103.7 degrees. A diffraction data set was collected in-house to 2.5 A resolution.
Morita
Hiroyuki
H
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Wanibuchi
Kiyofumi
K
Kato
Ryohei
R
Sugio
Shigetoshi
S
Abe
Ikuro
I
D003460:Crystallization; D018360:Crystallography, X-Ray; D036381:Diarylheptanoids / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D015394:Molecular Structure; D012275:Oryza / Q000201:enzymology*; D048630:Polyketide Synthases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D036381:Diarylheptanoids; D048630:Polyketide Synthases