Screen-printed poly(3,4-ethylenedioxythiophene) (PEDOT): A new electrochemical mediator for acetylcholinesterase-based biosensors.
This work describes the use of a PEDOT:PSS-based conductive polymer for designing AChE-based biosensors. The transducers were obtained directly by screen-printing a PEDOT:PSS suspension on the surface of thick film carbon electrodes. The obtained working electrodes showed a high conductivity when compared with electrodes modified with conventional mediators like cobalt phthalocyanine or tetracyanoquinodimethane. The PEDOT:PSS polymer was shown to be suitable for thiocholine oxidation, allowing the measurement of AChE activity at 100 mV vs Ag/AgCl. The high conductivity of PEDOT:PSS allowed the accurate detection of the organophosphate insecticide chlorpyrifos-oxon at concentrations as low as 4x10(-9)M, corresponding to an inhibition ratio of 5%.
Istamboulie
Georges
G
Université de Perpignan, Via Domitia, IMAGES, Bat. S, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France.
Sikora
Tomasz
T
Jubete
Elena
E
Ochoteco
Estibalitz
E
Marty
Jean-Louis
JL
Noguer
Thierry
T
D000110:Acetylcholinesterase; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004390:Chlorpyrifos / Q000032:analysis; D004553:Electric Conductivity; D004566:Electrodes; D007306:Insecticides / Q000032:analysis; D010755:Organophosphates / Q000032:analysis*; D011108:Polymers; D013860:Thiocholine / Q000378:metabolism
D019086:Bridged Bicyclo Compounds, Heterocyclic; D007306:Insecticides; D010755:Organophosphates; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D013860:Thiocholine; D000110:Acetylcholinesterase; D004390:Chlorpyrifos