title: Microevolution of Mycobacterium tuberculosis in a tuberculosis patient.
abstract: Five Mycobacterium tuberculosis isolates were obtained from three body sites from a Dutch patient. The isolates displayed a single genotype by 24-locus MIRU-VNTR typing (except for a single locus not amplified from one isolate) but were differentiated by small variations in IS6110 fingerprints, spoligotypes, 6 hypervariable MIRU-VNTR loci, and/or DiversiLab profiles, revealing patterns of microevolution in a clonal infection.
authors.affiliation: Department of Comparative Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. hajoj@kfshrc.edu.sa
authors.forename: Sahal A M
authors.initials: SA
authors.lastname: Al-Hajoj
authors.forename: Onno
authors.initials: O
authors.lastname: Akkerman
authors.forename: Ida
authors.initials: I
authors.lastname: Parwati
authors.forename: Saad
authors.initials: S
authors.lastname: al-Gamdi
authors.forename: Zeaur
authors.initials: Z
authors.lastname: Rahim
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
authors.forename: Jakko
authors.initials: J
authors.lastname: van Ingen
authors.forename: Philip
authors.initials: P
authors.lastname: Supply
authors.forename: Adri G M
authors.initials: AG
authors.lastname: van der Zanden
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009426:Netherlands; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial