PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica.
We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.
Navarre
William Wiley
WW
Department of Molecular Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada. william.navarre@utoronto.ca
Zou
S Betty
SB
Roy
Hervé
H
Xie
Jinglin Lucy
JL
Savchenko
Alexei
A
Singer
Alexander
A
Edvokimova
Elena
E
Prost
Lynne R
LR
Kumar
Runjun
R
Ibba
Michael
M
Fang
Ferric C
FC
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics; D008239:Lysine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D008250:Lysine-tRNA Ligase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010445:Peptide Elongation Factors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019779:Salmonella enterica / Q000378:metabolism / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D010445:Peptide Elongation Factors; D037521:Virulence Factors; C020746:factor EF-P; C100028:hypusine; D008250:Lysine-tRNA Ligase; D008239:Lysine