Seed-specific overexpression of antioxidant genes in Arabidopsis enhances oxidative stress tolerance during germination and early seedling growth.
Enzymatic and non-enzymatic antioxidants play important roles in the tolerance of abiotic stress. To increase the resistance of seeds to oxidative stress, At2S3 promoter from Arabidopsis was used to achieve overexpression of the antioxidants in a seed-specific manner. This promoter was shown to be capable of driving the target gene to have a high level of expression in seed-related organs, including siliques, mature seeds, and early seedlings, thus making its molecular farming applications in plants possible. Subsequently, genes encoding Mn-superoxide dismutase (MSD1), catalase (CAT1), and homogentisate phytyltransferase (HPT1, responsible for the first committed reaction in the tocopherol biosynthesis pathway) were overexpressed in Arabidopsis under the control of the At2S3 promoter. Double overexpressers co-expressing two enzymes and triple overexpressers were produced by cross pollination. Mn-SOD and total CAT activities, as well as gamma-tocopherol content, significantly increased in the corresponding overproduction lines. Moreover, single MSD1-transgene, double, and triple overexpressers displayed remarkably enhanced oxidative stress tolerance compared to wild type during seed germination and early seedling growth. Interestingly, an increase in the total CAT activity was also observed in the single MSD1-transgenic lines as a result of MSD1 overexpression. Together, the combined increase in Mn-SOD and CAT activities in seeds plays an essential role in the improvement of antioxidant capacity at early developmental stage in Arabidopsis.
Xi
Dong-Mei
DM
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, China.
Liu
Wu-Shuang
WS
Yang
Guo-Dong
GD
Wu
Chang-Ai
CA
Zheng
Cheng-Chao
CC
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination; D018384:Oxidative Stress; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000254:growth & development*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
D000975:Antioxidants; D029681:Arabidopsis Proteins; D018749:RNA, Plant; D002374:Catalase; D013482:Superoxide Dismutase; D019883:Alkyl and Aryl Transferases; C446531:HPT1 protein, Arabidopsis