Kinetic study for a stress testing of l,l-ethylenedicysteine by ultra-performance liquid chromatography/tandem mass spectrometry analysis.
This study proposed a stress testing to study oxidative stability and estimate the potential shelf-life of l,l-ethylenedicysteine (l,l-EC) under normal storage temperature condition (20-25 degrees C). l,l-EC was detected as a function of time at four different temperatures by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). The degradation of l,l-EC followed the first order kinetics, and the temperature-dependent kinetics was well described by the linear Arrhenius equation. The activation energy (E(a)) was calculated, and the shelf-life at 25 and 4 degrees C was predicted. The results are useful for the proper storage and quality evaluation of l,l-EC.
Sun
Xiaotao
X
Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
Qiao
Jinping
J
Zhu
Lin
L
Qiao
Hongwen
H
Zhong
Jian-Guo
JG
D002853:Chromatography, Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry / Q000379:methods*
C109063:ethylene dicysteine; D003545:Cysteine