title: Porcine reproductive and respiratory syndrome virus (PRRSV) could be sensed by professional beta interferon-producing system and had mechanisms to inhibit this action in MARC-145 cells.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) causes an economically important disease in swine-producing area, and interferon beta (IFN-beta) is the first responder against the animal virus infection. However, whether PRRSV could induce the production of IFN-beta is controversial. In this paper, we first time found that PRRSV could phosphorylate IFN-regulatory factor 3 (IRF-3) and weakly activate the IFN-beta promoter in MARC-145 cells in early infection, but the activations of IRF-3 and IFN-beta promoter were rapidly inhibited in the following infection. Furthermore, which components or products of the invading PRRSV cause PRRSV to inhibit IFN-beta promoter activity attracted our attentions. The obtained results showed that PRRSV nsp1 could inhibit Poly(I:C)-induced IFN-beta promoter activity in MARC-145 cells by down-regulating the protein level of IRF-3 and inhibiting the phosphorylation of IRF-3. In conclusion, our results suggested that PRRSV could be sensed by professional IFN-beta-producing system and had mechanisms to inhibit this action in MARC-145 cells.
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Xibao
authors.initials: X
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yubao
authors.initials: Y
authors.lastname: Zhi
authors.forename: Guangxu
authors.initials: G
authors.lastname: Xing
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D057131:Immune Evasion; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism; D016899:Interferon-beta / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000472:pathogenicity*; D013552:Swine; D014764:Viral Proteins / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D050838:Interferon Regulatory Factor-3; D014764:Viral Proteins; D037521:Virulence Factors; D016899:Interferon-beta