Novel streptavidin-functionalized silicon nanowire arrays for CD4+ T lymphocyte separation.
Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalized SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over approximately 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
Kim
Sung Tae
ST
Global Research Laboratory, Department of Biochemistry, Division of Brain Korea, College of Medicine, Korea University, Seoul 136-701, Korea.
Kim
Dong-Joo
DJ
Kim
Tae-Jin
TJ
Seo
Deok-Won
DW
Kim
Tae-Hong
TH
Lee
Seung-Yong
SY
Kim
Kwanghee
K
Lee
Kyung-Mi
KM
Lee
Sang-Kwon
SK
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002469:Cell Separation; D051379:Mice; D053770:Nanowires; D012825:Silicon / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
D019809:Streptavidin; D012825:Silicon