title: Network analysis of enzyme activities and metabolite levels and their relationship to biomass in a large panel of Arabidopsis accessions.
abstract: Natural genetic diversity provides a powerful resource to investigate how networks respond to multiple simultaneous changes. In this work, we profile maximum catalytic activities of 37 enzymes from central metabolism and generate a matrix to investigate species-wide connectivity between metabolites, enzymes, and biomass. Most enzyme activities change in a highly coordinated manner, especially those in the Calvin-Benson cycle. Metabolites show coordinated changes in defined sectors of metabolism. Little connectivity was observed between maximum enzyme activities and metabolites, even after applying multivariate analysis methods. Measurements of posttranscriptional regulation will be required to relate these two functional levels. Individual enzyme activities correlate only weakly with biomass. However, when they are used to estimate protein abundances, and the latter are summed and expressed as a fraction of total protein, a significant positive correlation to biomass is observed. The correlation is additive to that obtained between starch and biomass. Thus, biomass is predicted by two independent integrative metabolic biomarkers: preferential investment in photosynthetic machinery and optimization of carbon use.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany. sulpice@mpmip-golm.mpg.de
authors.forename: Ronan
authors.initials: R
authors.lastname: Sulpice
authors.forename: Sandra
authors.initials: S
authors.lastname: Trenkamp
authors.forename: Matthias
authors.initials: M
authors.lastname: Steinfath
authors.forename: Bjorn
authors.initials: B
authors.lastname: Usadel
authors.forename: Yves
authors.initials: Y
authors.lastname: Gibon
authors.forename: Hanna
authors.initials: H
authors.lastname: Witucka-Wall
authors.forename: Eva-Theresa
authors.initials: ET
authors.lastname: Pyl
authors.forename: Hendrik
authors.initials: H
authors.lastname: Tschoep
authors.forename: Marie Caroline
authors.initials: MC
authors.lastname: Steinhauser
authors.forename: Manuela
authors.initials: M
authors.lastname: Guenther
authors.forename: Melanie
authors.initials: M
authors.lastname: Hoehne
authors.forename: Johann M
authors.initials: JM
authors.lastname: Rohwer
authors.forename: Thomas
authors.initials: T
authors.lastname: Altmann
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Mark
authors.initials: M
authors.lastname: Stitt
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D020869:Gene Expression Profiling; D014644:Genetic Variation; D015999:Multivariate Analysis
chemical_list: D029681:Arabidopsis Proteins