Molecular cloning and characterization of the global regulator LaeA in Penicillium citrinum.
We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.
Xing
Wei
W
Institute of Modern Biopharmaceuticals, School of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400716, People's Republic of China.
Deng
Chao
C
Hu
Chang-Hua
CH
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D007526:Isoelectric Point; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010407:Penicillium / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*
D004271:DNA, Fungal; D005656:Fungal Proteins; D015534:Trans-Activators; C012258:mevastatin; D008148:Lovastatin