Changes of the unique odontogenic properties of rat apical bud cells under the developing apical complex microenvironment.
OBJECTIVE
To characterize the odontogenic capability of apical bud and phenotypical change of apical bud cells (ABCs) in different microenvironment.


METHODS
Incisor apical bud tissues from neonatal SD rat were dissected and transplanted into the renal capsules to determine their odontogenic capability. Meanwhile ABCs were cultured and purified by repeated differential trypsinization. Then ABCs were cultured with conditioned medium from developing apical complex cells (DAC-CM). Immunocytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and scanning electron microscope (SEM) were performed to compare the biological change ofABC treated with or without DAC-CM.


RESULTS
First we confirmed the ability of apical bud to form crown-like structure ectopically. Equally important, by using the developing apical complex (DAC) conditioned medium, we found the microenvironment created by root could abrogate the "crown" features of ABCs and promote their proliferation and differentiation.


CONCLUSIONS
ABCs possess odontogenic capability to form crown-like tissues and this property can be affected by root-produced microenvironment.
Fang
Jun
J
Department of Pedodontics, School of Stomatology, Fourth Military Medical University, Xi'an, China.
Tang
Liang
L
Liu
Xiao-hui
XH
Wen
Ling-ying
LY
Jin
Yan
Y
D000565:Ameloblasts / Q000166:cytology; D053523:Amelogenin / Q000032:analysis; D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D017690:Cell Transplantation; D017077:Culture Media, Conditioned; D003746:Dental Enamel Proteins / Q000032:analysis; D004847:Epithelial Cells / Q000166:cytology; D007150:Immunohistochemistry; D007180:Incisor / Q000166:cytology / Q000196:embryology; D053547:Keratin-14 / Q000032:analysis; D007668:Kidney / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D009805:Odontogenesis / Q000502:physiology*; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019227:Tooth Apex / Q000166:cytology*; D019228:Tooth Crown / Q000166:cytology; D014083:Tooth Germ / Q000166:cytology*
C098519:Ambn protein, rat; D053523:Amelogenin; D017077:Culture Media, Conditioned; D003746:Dental Enamel Proteins; D053547:Keratin-14