[Expression and clinical significance of VEGF and apoptosis in frozen-thawed mouse ovaries after transplantation].
OBJECTIVE
To investigate expression and clinical significance of VEGF and apoptosis in frozen-thawed mouse ovaries after transplantation.


METHODS
Ovaries from B6C2F1 (C57BL/6j x BALB/c) 4 week old mice were cryopreservation and the thawed ovaries were xenografted into kidney capsules of 8-12 week old adult male mice. The grafted were recovered 1 d, 2 d and 7 d after transplantation respectively, the grafts and frozen-thawed were removed for follicle counting and immunohistochemically, ultrastructure, and detection of the mRNA expression of vascular endothelial growth factor(VEGF).


RESULTS
The follicle numbers were decreased gradually after transplantation,the cell apoptosis increased especially in 48 h after transplantation. Transmission electron microscopy (TEM) showed the tissue damaged was severest 48 h after transplantation; the expression of VEGF increased after transplantation, peaked on day 7, the mRNA expression of VEGF120 and VEGF188 was more on 48 h after transplantation, decreased on day 7 (P < 0.05).


CONCLUSIONS
The number of follicles was decreased after transplantation, the apoptosis index was increased especially in 48 h after transplantation; the expression of VEGF increased after transplantation, an increase in the VEGF188 and VEGF164 isoform might suggest the positive effect in the early stages of angiogenesis.
Tang
Fei
F
Reproduction Medicine Center, the First Affiliated Hospital of Wenzhou Medical College, China.
Zhang
Chan
C
Wang
Xiao-jiao
XJ
Wu
Dan-dan
DD
Zhou
Ying
Y
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015925:Cryopreservation; D005260:Female; D008297:Male; D051379:Mice; D009926:Organ Preservation / Q000009:adverse effects; D006080:Ovarian Follicle / Q000473:pathology; D010053:Ovary / Q000473:pathology / Q000637:transplantation*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse