title: [Characterization of biocompatible CdTe/znte quantum dots and its application in cell labeling].
abstract: Water-soluble CdTe/ZnTe core-shell quantum dots (QDs) coated with L-cysteine were synthesized in low-temperature aqueous-phase one-pot approach. The authors measured the spectral characteristics of QDs at different pH in various buffer solutions and under different excitation laser powers. The primary results show that the absorption spectra of QDs approximately overlap and the fluorescence spectra peaks have no shift in different pH solution. The fluorescence intensity increased linearly with increasing pH. With the incubation time in borate buffer solution, the fluorescence intensity decreased a little. Under strong power laser, the QDs were photobleached rapidly. However, QDs are strongly anti-photobleaching under appropriate laser power (< 100 microW). Thus, such QDs have good biological stability and optical stability. By conjugating the QDs with transferrin protein and constructing the targeted fluorescent nanoparticles, the authors labelled the HeLa cell successfully. Photobleaching experiments in vivo show that microenvironment inside cells affect the stability and accelerate the photobleaching of QDs.
authors.affiliation: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronics Engineering, Shenzhen University, Shenzhen 518060, China. luxiai@hotmail.com
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Dan-Ni
authors.initials: DN
authors.lastname: Chen
authors.forename: Jun-Le
authors.initials: JL
authors.lastname: Qu
authors.forename: Jian-Tai
authors.initials: JT
authors.lastname: Yang
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Luo
authors.forename: Indrajit
authors.initials: I
authors.lastname: Roy
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Xiao-Tan
authors.initials: XT
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.forename: N Paras
authors.initials: NP
authors.lastname: Prasad
authors.forename: Gai-Xia
authors.initials: GX
authors.lastname: Xu
authors.forename: Han-Ben
authors.initials: HB
authors.lastname: Niu
mesh_terms: D001672:Biocompatible Materials; D019187:Cadmium Compounds; D003545:Cysteine; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013691:Tellurium; D014867:Water
chemical_list: D001672:Biocompatible Materials; D019187:Cadmium Compounds; D014867:Water; D003545:Cysteine; D013691:Tellurium