CC-chemokine MIP-1α in the spinal cord contributes to nerve injury-induced neuropathic pain.
We investigated the involvement of spinal macrophage inflammatory protein-1α (MIP-1α), an inflammatory chemokine, in partial sciatic nerve ligation (PSL)-induced neuropathic pain in mice. PSL increased MIP-1α mRNA levels as well as levels of the MIP-1α receptor, CCR1, but not CCR5 in the spinal dorsal horn. PSL-induced tactile allodynia and thermal hyperalgesia were prevented by intrathecal (i.t.) injection of a neutralizing antibody of MIP-1α (2ng). Recombinant MIP-1α (10pmol, i.t.) elicited long-lasting tactile allodynia and thermal hyperalgesia in naïve mice. These results suggest that peripheral nerve injury elicits the up-regulation of spinal MIP-1α and CCR1 to participate in neuropathic pain.
Kiguchi
Norikazu
N
Department of Pharmacology, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-0012, Japan.
Kobayashi
Yuka
Y
Maeda
Takehiko
T
Saika
Fumihiro
F
Kishioka
Shiroh
S
D000704:Analysis of Variance; D000818:Animals; D054405:Chemokine CCL3 / Q000378:metabolism*; D006930:Hyperalgesia / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009437:Neuralgia / Q000378:metabolism* / Q000503:physiopathology; D010147:Pain Measurement; D054389:Receptors, CCR1 / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism* / Q000503:physiopathology; D013116:Spinal Cord / Q000378:metabolism* / Q000503:physiopathology
C515301:Ccr1 protein, mouse; D054405:Chemokine CCL3; D054389:Receptors, CCR1