title: Electroporation enhances immune responses and protection induced by a bovine viral diarrhea virus DNA vaccine in newborn calves with maternal antibodies.
abstract: Bovine viral diarrhea virus (BVDV) is one of the major pathogens in cattle. In this study, newborn calves with maternal antibodies were vaccinated with a BVDV DNA vaccine, either by conventional intramuscular (IM) injection or with the TriGrid™ Delivery System for IM delivery (TDS-IM). The calves vaccinated with the TDS-IM developed more rapidly and effectively BVDV-specific humoral and cell-mediated immune responses in the presence of maternal antibodies. Overall, the immune responses induced by delivery with the TDS-IM remained stronger than those elicited by conventional IM injection of the BVDV DNA vaccine. Accordingly, electroporation-mediated delivery of the BVDV DNA vaccine resulted in close to complete protection from clinical signs of disease, while conventional IM administration did not fully prevent morbidity and mortality following challenge with BVDV-2. These results demonstrate the TDS-IM to be effective as a delivery system for a BVDV DNA vaccine in newborn calves in the presence of maternal antibodies, which supports the potential of electroporation as a delivery method for prophylactic DNA vaccines.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada. vandenhurk@sask.usask.ca
authors.forename: Sylvia
authors.initials: S
authors.lastname: van Drunen Littel-van den Hurk
authors.forename: Zoe
authors.initials: Z
authors.lastname: Lawman
authors.forename: Don
authors.initials: D
authors.lastname: Wilson
authors.forename: Alain
authors.initials: A
authors.lastname: Luxembourg
authors.forename: Barry
authors.initials: B
authors.lastname: Ellefsen
authors.forename: Jan V
authors.initials: JV
authors.lastname: van den Hurk
authors.forename: Drew
authors.initials: D
authors.lastname: Hannaman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control*; D002417:Cattle / Q000276:immunology; D030242:Diarrhea Virus 2, Bovine Viral / Q000276:immunology*; D018274:Electroporation; D056724:Immunity, Humoral; D007112:Immunity, Maternally-Acquired; D009500:Neutralization Tests / Q000662:veterinary; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines