title: Absence of mitochondrial progesterone receptor polymorphisms in women with spontaneous preterm birth.
abstract: OBJECTIVE
  The truncated mitochondrial progesterone receptor (PR-M) is homologous to nuclear PRs with the exception of an amino terminus hydrophobic membrane localization sequence, which localizes PR-M to mitochondria. Given the matrilineal inheritance of both spontaneous preterm birth (SPTB) and the mitochondrial genome, we hypothesized that (a) PR-M is polymorphic and (b) PR-M localization sequence polymorphisms could result in variable progesterone-mitochondrial effects and variable responsiveness to progesterone prophylaxis.
  
  
  METHODS
  Secondary analysis of DNA from women enrolled in a multicenter, prospective, study of 17 alpha-hydroxyprogesterone caproate (17OHPC) versus placebo for the prevention of recurrent SPTB. DNA was extracted from stored saliva.
  
  
  RESULTS
  The PR-M localization sequence was sequenced on 344 patients. Sequences were compared with the previously published 48 base-pair sequence, and all were identical.
  
  
  CONCLUSIONS
  We did not detect genetic variation in the mitochondrial localization sequence of the truncated PR-M in a group of women at high risk for SPTB.
authors.affiliation: Eunice Kennedy Shriver NICHD MFMU Network, Bethesda, MD, USA. tracy.manuck@hsc.utah.edu
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Manuck
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Price
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Thom
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Meis
authors.forename: Mitchell P
authors.initials: MP
authors.lastname: Dombrowski
authors.forename: Baha
authors.initials: B
authors.lastname: Sibai
authors.forename: Catherine Y
authors.initials: CY
authors.lastname: Spong
authors.forename: Dwight J
authors.initials: DJ
authors.lastname: Rouse
authors.forename: Jay D
authors.initials: JD
authors.lastname: Iams
authors.forename: Hyagriv N
authors.initials: HN
authors.lastname: Simhan
authors.forename: Mary J
authors.initials: MJ
authors.lastname: O'Sullivan
authors.forename: Menachem
authors.initials: M
authors.lastname: Miodovnik
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Leveno
authors.forename: Deborah
authors.initials: D
authors.lastname: Conway
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Wapner
authors.forename: Marshall
authors.initials: M
authors.lastname: Carpenter
authors.forename: Brian
authors.initials: B
authors.lastname: Mercer
authors.forename: Susan M
authors.initials: SM
authors.lastname: Ramin
authors.forename: John M
authors.initials: JM
authors.lastname: Thorp
authors.forename: Alan M
authors.initials: AM
authors.lastname: Peaceman
mesh_terms: D000595:Amino Acid Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011247:Pregnancy; D047928:Premature Birth / Q000235:genetics*; D011446:Prospective Studies; D011980:Receptors, Progesterone / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D011980:Receptors, Progesterone