Accumulation of copper in Trichoderma reesei transformants, constructed with the modified Agrobacterium tumefaciens-mediated transformation technique.
A modified Agrobacterium tumefaciens-mediated transformation method was established for the construction of mutants with improved copper tolerance and accumulation capability in Trichoderma reesei. One transformant, AT01, exhibited the highest copper accumulation capability. With copper at 0.7 mM, AT01 removed 13 mg copper/g biomass (removal rate of 96%), whereas the wild-type strain removed only 6 mg copper/g biomass (removal rate of 50%). Optimal conditions were a pH of 5.0 at 28°C. The pigment change of Trichoderma mycelia was a potential indicator of copper accumulation. Electron microscopy revealed that copper was mainly accumulated in cell vacuoles.
Fu
Kehe
K
Department of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.
Liu
Lixing
L
Fan
Lili
L
Liu
Tong
T
Chen
Jie
J
D016960:Agrobacterium tumefaciens / Q000235:genetics; D018533:Biomass; D003300:Copper / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D025282:Mycelium / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature; D014170:Transformation, Genetic; D014242:Trichoderma / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure
D003300:Copper