CD45RA expression on HCMV-specific effector memory CD8+ T cells is associated with the duration and intensity of HCMV replication after transplantation.
In this cross-sectional study on 42 solid organ transplant recipients, the association of kinetics of human cytomegalovirus (HCMV) replication and EMRA HCMV-specific CD8+ T cells was investigated. Correlation was observed between the duration of HCMV replication after transplantation and CD45RA+CD27- (r=0.609; p=0.004), CD45RA+CD28- (r=0.579; p=0.008) or CD45RA+CCR7- (r=0.488; p=0.029) HCMV-specific CD8+ T cells percentages. In the multivariate regression analyses, CD45RA+CD27-, CD45RA+CD28- or CD45RA+CCR7- HCMV-specific CD8+ T cells percentages increased 5.58% (p=0.001), 5.35% (p=0.001) or 4.49% (p=0.012), respectively, with every 10-day increase in the duration of HCMV replication. Moreover, CD45RA+CD27- or CD45RA+CD28- frequencies increased 4.16% (p=0.024) or 3.58% (p=0.049), respectively, with every unity increase in log(10) genomes/mL. These observations support the major association between the frequency of EMRA HCMV-specific CD8+ T cells and the duration of post-transplant HCMV replication episodes in solid organ transplantation recipients.
Cantisán
Sara
S
Instituto Maimónides de Investigación Biomédica de Córdoba, Hospital Universitario Reina Sofía, Universidad de Córdoba, Spanish Network for Research in Infectious Diseases (REIPI), Spain. sacanti@hotmail.com
Solana
Rafael
R
Lara
Rosario
R
Rodríguez-Benot
Alberto
A
Vaquero
Jose Manuel
JM
Gutiérrez-Aroca
Juan
J
Gayoso
Inmaculada
I
Montejo
Miguel
M
Rivero
Antonio
A
Torre-Cisneros
Julián
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D003430:Cross-Sectional Studies; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D016040:Lung Transplantation / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D014019:Tissue Donors; D014180:Transplantation; D019562:Viral Load / Q000276:immunology; D014779:Virus Replication / Q000276:immunology*; D055815:Young Adult
D000914:Antibodies, Viral; D017493:Leukocyte Common Antigens