title: Encoding a superantigen by Staphylococcus aureus does  not affect clinical characteristics of infected atopic  dermatitis lesions.
abstract: BACKGROUND
  Bacterial infection with Staphylococcus aureus is a known trigger for the worsening of atopic dermatitis (AD). Staphylococcal superantigens have been theorized to make a potential contribution to this worsening of AD seen with infection.
  
  
  OBJECTIVE
  We sought to assess whether encoding a superantigen by S. aureus affects the inflammatory characteristics of impetiginized AD skin lesions.
  
  
  METHODS
  Fifty-two children with clinically impetiginized lesions of AD which were positive for S. aureus were enrolled in this study. A lesion was graded clinically using the Eczema Area and Severity Index (EASI), and then wash fluid was obtained from the lesion for quantitative bacterial culture, and measurement of bacterial products lipoteichoic acid and staphylococcal protein A and cytokines. The staphylococcal isolate was tested for antibiotic susceptibilities and the presence of a superantigen.
  
  
  RESULTS
  Fifty-four per cent (28 of 52) of the staphylococcal isolates encoded a superantigen. The presence of a superantigen had no significant effect on EASI score, amounts of bacterial products or inflammatory cytokines in the AD lesion.
  
  
  CONCLUSIONS
  These studies suggest that the expression of a superantigen by S. aureus alone does not play an important role in the increased skin inflammation associated with staphylococcal infection in childhood AD.
authors.affiliation: Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kozman
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.forename: P
authors.initials: P
authors.lastname: Bina
authors.forename: C
authors.initials: C
authors.lastname: Saha
authors.forename: W
authors.initials: W
authors.lastname: Yao
authors.forename: M H
authors.initials: MH
authors.lastname: Kaplan
authors.forename: J B
authors.initials: JB
authors.lastname: Travers
mesh_terms: D001426:Bacterial Proteins / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D004485:Eczema / Q000150:complications / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012720:Severity of Illness Index; D013207:Staphylococcal Skin Infections / Q000276:immunology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D016207:Cytokines; D018089:Superantigens