title: Fluorescent sensing of colloidal CePO4:Tb nanorods for rapid, ultrasensitive and selective detection of vitamin C.
abstract: Vitamin C is an essential biological molecule for living organisms. The detection of vitamin C is always required due to its wide use in chemical, biological and pharmaceutical engineering. Here, we established a novel sensing system for rapid, ultrasensitive and highly selective detection of vitamin C based on a 'turn-on' fluorescent method. The turn-on fluorescent sensing system was built up of a colloidal CePO(4):Tb nanocrystalline solution with its fluorescence quenched by KMnO(4). The addition of vitamin C leads to a linear increase of fluorescence. The sensing principle of nanocrystalline CePO(4):Tb is based on a redox reaction via simply modulating the surface chemistry of nanocrystals. Our present sensing system for vitamin C exhibits a rapid response rate of less than 2 min, and highly selective and ultrasensitive detection with a detection limit of 108 nM, which is two orders of magnitude lower than that acquired by previously reported methods. The repeated reversibility of fluorescence quenching/recovery with time revealed a high reproducibility and long-term stability of our sensing materials. Furthermore, our developed sensing material overcomes the disadvantages such as complex surface modification/immobilization and serious biotoxicity compared to quantum-dot-based fluorescent sensing systems.
authors.affiliation: World Premier International Research (WPI) Center Initiative on Materials Nanoarchitronics (MANA), National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan. weihdi@yahoo.com.cn
authors.forename: Weihua
authors.initials: W
authors.lastname: Di
authors.forename: Naoto
authors.initials: N
authors.lastname: Shirahata
authors.forename: Haibo
authors.initials: H
authors.lastname: Zeng
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakka
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry*; D002563:Cerium / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011196:Potassium Permanganate; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D003102:Colloids; D010710:Phosphates; D011196:Potassium Permanganate; D013725:Terbium; D002563:Cerium; D001205:Ascorbic Acid; C048083:cerium phosphate