Lipid chain branching at the iso- and anteiso-positions in complex Chlamydia membranes: a molecular dynamics study.
Membranes in the intracellular eubacterial parasite Chlamydia trachomatis consist of the elementary body (EB) and reticular body (RB), and contain methyl branches at the iso- and anteiso-positions for some phospholipid chains. Acyl chain branching is the focus of this study. Molecular dynamics simulations were used to study bilayers of 1-13-methylpentadecanoyl-2-palmitoyl-phosphatidylcholine (13-MpPPC), 1-14-methylpentadecanoyl-2-palmitoyl-phosphatidylcholine (14-MpPPC), and diphytanoylphosphatidylcholine (DPhPC). These three membranes were simulated at 323K and simulations of DPhPC at 298K were also performed for better comparison to existing experimental data. Two simulations of representative EB and RB membranes of C. trachomatis composed of nine different lipid components were performed at 310.15K, to accurately reflect compositions determined by experiment and physiological conditions. Based on nearly 0.5μs of simulation data, we report that branching increases average lipid surface area, area elastic moduli, and lipid axial relaxation times, while decreasing lipid chain order. Branching also has a distinct effect on electron density profiles. Due to their high cholesterol concentrations, the EB and RB membranes were found to have relatively high area elastic moduli, which may have important biological implications.
Lim
Joseph B
JB
Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA.
Klauda
Jeffery B
JB
D001703:Biophysics / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism*; D002689:Chlamydia / Q000378:metabolism*; D004548:Elasticity; D004583:Electrons; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D013499:Surface Properties; D013997:Time Factors
D008051:Lipid Bilayers; D008055:Lipids