title: Removal of Salmonella Enteritidis from commercial unpasteurized liquid egg white using pilot scale cross flow tangential microfiltration.
abstract: Effectiveness of a cross flow microfiltration (MF) process for removal of a cocktail of Salmonella enterica serovar Enteritidis species from commercial unpasteurized liquid egg white (LEW) from a local egg breaking plant, while maintaining its functional properties was evaluated. To facilitate MF, LEW was wedge screened, homogenized and then diluted (1:2 w/w) with distilled water containing 0.5% sodium chloride. Diluted unpasteurized LEW was inoculated with five strains of S. Enteritidis (ATCC 4931, ATCC BAA-708, ATCC 49215, ATCC 49218, and ATCC BAA-1045) to a level of approximately 10(7)CFU/mL of LEW and microfiltered using a ceramic membrane. Process parameters influencing egg white functional properties and pathogen removal efficiency were evaluated. Average permeates flux increased by almost 126% when pH of LEW was adjusted from pH 8 to pH 7 at 25 degrees C. Microbial removal efficiency was at least, on average, 6.8Log(10)CFU/mL (limit of detection < or =0.5Log(10)CFU/mL). Functional property analysis indicated that the MF process did not alter the foaming power of LEW.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional, Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. sudarsan.mukhopadhyay@ars.usda.gov
authors.forename: Sudarsan
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: Peggy M
authors.initials: PM
authors.lastname: Tomasula
authors.forename: John B
authors.initials: JB
authors.lastname: Luchansky
authors.forename: Anna
authors.initials: A
authors.lastname: Porto-Fett
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Call
mesh_terms: D002516:Ceramics; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004529:Egg White / Q000382:microbiology*; D005374:Filtration / Q000379:methods*; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods; D005525:Food-Processing Industry / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D010865:Pilot Projects; D012478:Salmonella Food Poisoning / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000302:isolation & purification*
chemical_list: D008567:Membranes, Artificial