title: In vivo anti-inflammatory effect and toxicological screening of Maytenus heterophylla and Maytenus senegalensis extracts.
abstract: Maytenus heterophylla (Eckl & Zeyh.) Robson and Maytenus senegalensis (Lam). Exell are two African medicinal plants used to treat painful and inflammatory diseases. We evaluated the in vivo (per os) anti-inflammatory activity of M. heterophylla leaf, stem and root extracts and of M. senegalensis leaf and stem extracts. Additionally, we assessed their in vivo acute and sub-acute toxicities. Anti-inflammatory activities of ethanol extracts were determined in Wistar albino rats, by the carrageenan-induced paw oedema method. Acute and sub-acute toxicity screening of the extracts was evaluated in adult male CD-6 mice. Leaf extracts of M. heterophylla and M. senegalensis exhibited significant anti-inflammatory activity (120 mg/kg, per os), reducing oedema by 51% and 35%, respectively. While M. heterophylla extracts at 1200 mg/kg have shown to be non-toxic, M. senegalensis extracts indicated some toxicity. Our results show a significant anti-inflammatory effect of both M. heterophylla and M. senegalensis leaf extracts in a local model of acute inflammation and suggest the absence of acute and sub-acute toxicity signs of the M. heterophylla leaf extract (but not of M. senegalensis). Ongoing studies will surely shed some light into the mechanism of action of this active extract and establish its chemical fingerprint.
authors.affiliation: iMed. UL, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.
authors.forename: G
authors.initials: G
authors.lastname: da Silva
authors.forename: M
authors.initials: M
authors.lastname: Taniça
authors.forename: J
authors.initials: J
authors.lastname: Rocha
authors.forename: R
authors.initials: R
authors.lastname: Serrano
authors.forename: E T
authors.initials: ET
authors.lastname: Gomes
authors.forename: B
authors.initials: B
authors.lastname: Sepodes
authors.forename: O
authors.initials: O
authors.lastname: Silva
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000633:toxicity*; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D006614:Hindlimb / Q000187:drug effects / Q000473:pathology; D008136:Longevity / Q000187:drug effects; D008297:Male; D029755:Maytenus / Q000737:chemistry*; D016488:Medicine, African Traditional; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts