title: Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions.
abstract: Reversible phosphoinositide phosphorylation provides a dynamic membrane code that balances opposing cell functions. However, in vivo regulatory relationships between specific kinases, phosphatases, and phosphoinositide subpools are not clear. We identified myotubularin (mtm), a Drosophila melanogaster MTM1/MTMR2 phosphoinositide phosphatase, as necessary and sufficient for immune cell protrusion formation and recruitment to wounds. Mtm-mediated turnover of endosomal phosphatidylinositol 3-phosphate (PI(3)P) pools generated by both class II and III phosphatidylinositol 3-kinases (Pi3K68D and Vps34, respectively) is needed to down-regulate membrane influx, promote efflux, and maintain endolysosomal homeostasis. Endocytosis, but not endolysosomal size, contributes to cortical remodeling by mtm function. We propose that Mtm-dependent regulation of an endosomal PI(3)P pool has separable consequences for endolysosomal homeostasis and cortical remodeling. Pi3K68D depletion (but not Vps34) rescues protrusion and distribution defects in mtm-deficient immune cells and restores functions in other tissues essential for viability. The broad interactions between mtm and class II Pi3K68D suggest a novel strategy for rebalancing PI(3)P-mediated cell functions in MTM-related human disease.
authors.affiliation: Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Michaella
authors.initials: M
authors.lastname: Velichkova
authors.forename: Joe
authors.initials: J
authors.lastname: Juan
authors.forename: Pavan
authors.initials: P
authors.lastname: Kadandale
authors.forename: Steve
authors.initials: S
authors.lastname: Jean
authors.forename: Inês
authors.initials: I
authors.lastname: Ribeiro
authors.forename: Vignesh
authors.initials: V
authors.lastname: Raman
authors.forename: Chris
authors.initials: C
authors.lastname: Stefan
authors.forename: Amy A
authors.initials: AA
authors.lastname: Kiger
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004330:Drosophila; D006706:Homeostasis; D008247:Lysosomes / Q000378:metabolism*; D008954:Models, Biological; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000378:metabolism*
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D054558:Protein Tyrosine Phosphatases, Non-Receptor; C099588:myotubularin