Immunhistochemical analysis for expression of calpain 1, calpain 2 and calpastatin in endometrial cancer.
BACKGROUND
Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important role in many processes including apoptosis, necrosis, ischaemia and exocytosis. The role of calpains in pathogenesis or further tumour progression has been proved in related studies. This study focused on the expression of the enzymes calpain 1, calpain 2 and the inhibitor calpastatin in normal and malignant endometrial tissue.


METHODS
Immunohistochemical stainings were performed on paraffin slices and staining intensity, percentage of positive cells and international ratio score were evaluated.


CONCLUSIONS
The endometrial carcinoma showed a higher expression of calpastatin than benign endometrial tissue.
Salehin
Darius
D
Department of Gynecology and Obstretics, Helios Hospital Krefeld, Lutherplatz 40, 47805 Krefeld, Germany. darius.salehin@helios-kliniken.de
Fromberg
Iris
I
Haugk
Christina
C
Dohmen
Birgit
B
Georg
Thomas
T
Bohle
Rainer Maria
RM
Bauerschlag
Dirk
D
Maass
Nicolai
N
Friedrich
Michael
M
D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D002154:Calpain / Q000096:biosynthesis*; D016889:Endometrial Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging
D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain; C569870:CAPN2 protein, human