Effects of 3-hydroxy-3-methylglutaryl-coenzyme a reductase inhibitor pravastatin on membrane lipids and membrane associated functions of Methanothermobacter thermautotrophicus.
The role of archaeal membrane and its lipid constituents was investigated in bioenergetic functions of Methanothermobacter thermautotrophicus. The effects were determined of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor, pravastatin, on lipid composition, and its impact on some bioenergetic functions of treated cells. Pravastatin remarkably inhibited the growth of M. thermautotrophicus. On membrane level, pravastatin treatment modulated the composition of the mixture of squalene and hydrosqualene derivatives as well as the activities of ATPase, A1Ao-ATP synthase and Na+/H+ antiporter. SDS-PAGE of chloroform-methanol extracts of membranes from control and pravastatin-treated cells revealed changes in the amount of AtpK proteolipids, which suggests that pravastatin modifies cell-membrane composition, hereby modulating the properties of some membrane-bound enzymes participating in energy transformation in methanoarchaea.
Nováková
Z
Z
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 900 28, Ivanka pri Dunaji, Slovakia.
Blasko
J
J
Hapala
I
I
Smigán
P
P
D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004734:Energy Metabolism / Q000187:drug effects; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008563:Membrane Lipids / Q000032:analysis*; D017013:Methanobacteriaceae / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D017035:Pravastatin / Q000494:pharmacology*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D013185:Squalene / Q000032:analysis
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008563:Membrane Lipids; D017923:Sodium-Hydrogen Exchangers; D013185:Squalene; D006180:Proton-Translocating ATPases; D017035:Pravastatin