title: M-marker 2009, a marker set for mapping medaka mutants using PCR length polymorphisms with an automated microchip gel electrophoresis system.
abstract: We have developed a highly effective marker set for assigning medaka mutants to specific linkage groups (LG) in bulk segregation analysis by PCR product length polymorphisms (PLPs). The marker set permits analysis using an automated microchip electrophoresis system as well as conventional agarose gel electrophoresis.
authors.affiliation: National Institute for Basic Biology, Laboratory of Bioresources, The Graduate University for Advanced Studies (SOKRNDAI), Okazaki, Japan. tekimura@nibb.ac.jp
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Kimura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Naruse
mesh_terms: D000818:Animals; D001331:Automation; D002874:Chromosome Mapping / Q000379:methods*; D020090:Chromosome Segregation / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D005819:Genetic Markers; D009154:Mutation / Q000235:genetics*; D009990:Oryzias / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*
chemical_list: D005819:Genetic Markers