In vivo analysis of gene knockdown in tetracycline-inducible shRNA mice.
Expression of small hairpin RNA (shRNA) in mammalian cells can trigger potent RNAi-mediated gene silencing. The dominant-acting RNAi can often result in phenotypes similar to that of a null allele. Moreover, the generation of shRNA knockdown mice and subsequent phenotypic analysis can be achieved in a condensed timeline compared to that of conventional gene-targeting knockout strategies. Here, we discuss methods for the in vivo analysis of gene function in adult mouse tissues following tetracycline-induced RNA knockdown in a single-copy inducible polymerase III promoter-driven shRNA system.
Raymond
Christopher S
CS
Merck & Co., Inc., Rahway, New Jersey, USA.
Zhu
Lei
L
Vogt
Thomas F
TF
Shin
Myung K
MK
D000818:Animals; D004318:Doxycycline / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing / Q000502:physiology*; D051379:Mice; D034622:RNA Interference / Q000187:drug effects / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology*
D034741:RNA, Small Interfering; D013752:Tetracycline; D004318:Doxycycline