title: The porcine tribbles homolog 3 (TRIB3) gene: Identification of a missense mutation and association analysis with meat quality and production traits in Italian heavy pigs.
abstract: TRIB3 plays an important role in energy metabolism. This work aimed to study the porcine tribbles homolog 3 (TRIB3) gene and to evaluate its association with meat quality and carcass traits in pigs. By sequencing a portion of the porcine TRIB3 gene two single nucleotide polymorphisms (SNPs) in the first coding exon (one synonymous SNP: c.132 T>C; and one missense mutation: c.146C>T, p.P49L) were identified. The two polymorphisms were in complete linkage disequilibrium. In silico analysis of the p.P49L mutation suggested that it could have functional effects. Association studies in four groups of pigs (651 animals in total) indicated that this gene marker was associated with back fat thickness in Italian Large White and Italian Duroc pigs in two different experimental designs (P<0.1 and P<0.05). This polymorphism tended to be associated with lactate content of the semimembranosus muscle (P<0.1). Among several other tissues, TRIB3 is expressed in fat and skeletal muscle.
authors.affiliation: DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Via F.lli Rosselli 107, 42123 Reggio Emilia, Italy. luca.fontanesi@unibo.it
authors.forename: L
authors.initials: L
authors.lastname: Fontanesi
authors.forename: M
authors.initials: M
authors.lastname: Colombo
authors.forename: E
authors.initials: E
authors.lastname: Scotti
authors.forename: L
authors.initials: L
authors.lastname: Buttazzoni
authors.forename: F
authors.initials: F
authors.lastname: Bertolini
authors.forename: S
authors.initials: S
authors.lastname: Dall'Olio
authors.forename: R
authors.initials: R
authors.lastname: Davoli
authors.forename: V
authors.initials: V
authors.lastname: Russo
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology / Q000737:chemistry; D050154:Adiposity / Q000235:genetics; D000818:Animals; D015870:Gene Expression; D019344:Lactic Acid / Q000032:analysis; D015810:Linkage Disequilibrium; D008460:Meat / Q000032:analysis* / Q000592:standards; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry*; D020125:Mutation, Missense; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D019344:Lactic Acid