Taenia solium: immune response against oral or systemic immunization with purified recombinant calreticulin in mice.
Recombinant functional Taenia solium calreticulin (rTsCRT) confers different degrees of protection in the experimental model of intestinal taeniosis in hamsters. The aim of this study was to evaluate the immune response induced after oral or systemic immunization with an electroeluted rTsCRT in BALB/c mice. Oral immunization elicited high fecal IgA and the production of IL-4 and IL-5 by mesenteric lymph node cells after in vitro stimulation with rTSCRT, indicating a Th2 response. Mice subcutaneously immunized produced high amounts of serum IgG, being IgG1 (Th2-related) the predominant isotype, while in vitro stimulated spleen cells synthesized IL-4, IL-5 and also IFN-γ, indicating a mixed Th1/Th2 cellular response after systemic immunization. Our data show that purified rTsCRT induces polarized Th2 responses after oral immunization of mice, a common characteristic of protective immunity against helminths and, consequently, a desirable hallmark in the search for a vaccine.
Fonseca-Coronado
Salvador
S
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Coyoacán, México, DF 04510, Mexico. pelfor@hotmail.com
Ruiz-Tovar
Karina
K
Pérez-Tapia
Mayra
M
Mendlovic
Fela
F
Flisser
Ana
A
D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D015153:Blotting, Western; D037282:Calreticulin / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007279:Injections, Subcutaneous; D007421:Intestine, Small / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008643:Mesentery; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000276:immunology; D041201:Taenia solium / Q000276:immunology*; D013622:Taeniasis / Q000517:prevention & control
D000909:Antibodies, Helminth; D037282:Calreticulin; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins