title: [Chromogenic medium for detection of enterobacteria producing beta-lactamases in North-Eastern Romania].
abstract: We evaluated the performances and analyzed practicability of an ESBL-detecting chromogenic agar medium (chromID ESBL, bioMerieux) for the screening of ESBL production.
  
  
  METHODS
  We have tested 112 enterobacteria strains, isolated in 2009 year, in North Eastern region of Romania. Each bacterial strain was cultured on a prepared plate chromogenic medium and the results were recorded after incubation at 37 degrees C for 18 to 24 h or, if necessary, for 48 h. We calculated the sensitivities, specificities, and positive and negative predictive values for this medium, using as gold standard ceftzidime or cefotaxime and clavulanate combination disks on Iso-Sensitest agar. K. pneumoniae ATCC 700603 and E. coli ATCC 25922 were used as the positive and negative control for ESBL production, respectively.
  
  
  RESULTS
  For all tested strains, the chromID ESBL agar showed a high sensitivity (97%), but a low specificity (66%). The positive and negative predictive values were 98% and 57%, respectively. The chromID ESBL had false-positive results for 2 confirmed ESBL-positive strains.
  
  
  CONCLUSIONS
  The very good sensitivity of the chromID ESBL agar ESBL detection represent a convenient method for the recovery and identification of ESBL-producing enterobacteria.
authors.affiliation: Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi, Facultatea de Medicină, Disciplina de Microbiologie.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tuchiluş
authors.forename: Antonia
authors.initials: A
authors.lastname: Poiată
authors.forename: D
authors.initials: D
authors.lastname: Buiuc
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D002439:Cefotaxime / Q000494:pharmacology; D002863:Chromogenic Compounds / Q000378:metabolism*; D019818:Clavulanic Acid / Q000494:pharmacology; D003470:Culture Media; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology; D007711:Klebsiella pneumoniae / Q000201:enzymology; D011237:Predictive Value of Tests; D012383:Romania; D013997:Time Factors; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D002863:Chromogenic Compounds; D003470:Culture Media; D019818:Clavulanic Acid; D001618:beta-Lactamases; D002439:Cefotaxime