Transcription factors in light and circadian clock signaling networks revealed by genomewide mapping of direct targets for neurospora white collar complex.
Light signaling pathways and circadian clocks are inextricably linked and have profound effects on behavior in most organisms. Here, we used chromatin immunoprecipitation (ChIP) sequencing to uncover direct targets of the Neurospora crassa circadian regulator White Collar Complex (WCC). The WCC is a blue-light receptor and the key transcription factor of the circadian oscillator. It controls a transcriptional network that regulates ∼20% of all genes, generating daily rhythms and responses to light. We found that in response to light, WCC binds to hundreds of genomic regions, including the promoters of previously identified clock- and light-regulated genes. We show that WCC directly controls the expression of 24 transcription factor genes, including the clock-controlled adv-1 gene, which controls a circadian output pathway required for daily rhythms in development. Our findings provide links between the key circadian activator and effectors in downstream regulatory pathways.
Smith
Kristina M
KM
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, 97331-7305, USA.
Sancar
Gencer
G
Dekhang
Rigzin
R
Sullivan
Christopher M
CM
Li
Shaojie
S
Tag
Andrew G
AG
Sancar
Cigdem
C
Bredeweg
Erin L
EL
Priest
Henry D
HD
McCormick
Ryan F
RF
Thomas
Terry L
TL
Carrington
James C
JC
Stajich
Jason E
JE
Bell-Pedersen
Deborah
D
Brunner
Michael
M
Freitag
Michael
M
D047369:Chromatin Immunoprecipitation; D057906:Circadian Clocks; D002940:Circadian Rhythm; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D016681:Genome, Fungal / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D008027:Light; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D014157:Transcription Factors