[Development and genetic polymorphism of abnormal pronuclear zygotes after intracytoplasmic sperm injection].
OBJECTIVE
To compare the development of abnormal pronuclear zygotes after intracytoplasmic sperm injection (ICSI) and analyze their genetic polymorphism.


METHODS
Four hundred and ninety three abnormal pronuclear zygotes after ICSI were divided into three groups based on the number of pronuclei: 347 nonpronuclear oocytes, 71 monopronuclear zygotes and 75 multipronuclear zygotes. All of them were cultured in the medium of Vitrolife G5 series(TM). Sixteen short tandem repeats (STR) of seven blastocysts were then analyzed by ABI3100.


RESULTS
The cleavage rate of nonpronuclear group (25.4%) was lower than that of the others (P<0.01), the proportion of blocked embryos in nonpronuclear group (48.9%) was significantly higher than that of the others (P<0.05), but the blastocyst rate showed no significant difference in three groups (P>0.05). The genetic polymorphism of the 16 STRs showed that the blastocysts from the nonpronuclear and multipronuclear were diploid, and one of the blastocysts from nonpronuclear oocyte was Y-bearing.


CONCLUSIONS
The zygotes with abnormal pronuclei after ICSI might have development potential, and the blastocysts from nonpronuclear oocytes and multipronuclear zygotes could be diploid.
Zhang
Wen-hong
WH
Department of Reproductive Medicine, the Third Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong, 510150 PR China.
Du
Hong-zi
HZ
Li
Li
L
Huang
Yu-ling
YL
Shi
Yu
Y
Li
Shao-ying
SY
Zhang
Wei-liang
WL
Sun
Xiao-fang
XF
Long
Xiao-lin
XL
D001755:Blastocyst / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects*; D020080:Tandem Repeat Sequences; D015053:Zygote / Q000502:physiology*