Modified agarose gel for high-performance Southern blotting of short DNA fragments.
Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolved fractionation of short DNA fragments comparable to that from a high-concentration agarose gel could be obtained using a low-concentration agarose gel with a small zone of high-concentration agarose, and that the resulting DNA transfer is highly efficient and rapid.
Huang
Wei-Hong
WH
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Huang
Xiu-Ying
XY
Sun
Fang-Zhen
FZ
D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009707:Nucleosomes / Q000378:metabolism
D009707:Nucleosomes; D004247:DNA