title: Mitogenic activity of CEL-I, an N-acetylgalactosamine (GalNAc)-specific C-type lectin, isolated from the marine invertebrate Cucumaria echinata (Holothuroidea).
abstract: An N-acetylgalactosamine (GalNAc)-specific Ca(2+)-dependent lectin (C-type lectin), isolated from the marine invertebrate Holothuroidea (Cucumaria echinata), CEL-I, showed potent mitogenic activity toward normal mouse spleen cells. The mitogenic activity of CEL-I, which reached a maximum at 100 microg/ml, was inhibited by GalNAc in a concentration-dependent manner. The mitogenic effect of CEL-I at 10 microg/ml on T cell- enriched splenocytes was at a similar level due to a well-known T cell mitogen, concanavalin A (Con A), at 10 microg/ml. Furthermore, CEL-I evoked a mitogenic response from nude mouse spleen cells, while no significant effects of Con A on this cell population were observed over a wide range of concentrations. These results suggest that CEL-I is a potent mitogenic lectin with the ability to stimulate both T and B cells.
authors.affiliation: Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Nagasaki, Japan.
authors.forename: Zedong
authors.initials: Z
authors.lastname: Jiang
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamanishi
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Hatakeyama
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D000818:Animals; D048016:Cucumaria / Q000737:chemistry*; D037181:Lectins, C-Type / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051379:Mice; D008934:Mitogens / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013379:Substrate Specificity
chemical_list: D037181:Lectins, C-Type; D008934:Mitogens; D000116:Acetylgalactosamine