title: Simultaneous determination of first-line anti-tuberculosis drugs by capillary zone electrophoresis using direct UV detection.
abstract: An alternative methodology for simultaneous analysis of ethambutol, isoniazid, rifampicin and pyrazinamide in pharmaceutical formulations by capillary zone electrophoresis under UV direct detection with an analysis time of 8.0 min is proposed. Background running was based on the effective mobility curve of the analytes and an optimum separation condition was achieved using a 3(3) Box-Behnken design, with Brij 35, Cu(2+) and acetic acid/sodium acetate buffer as factors. An electrolyte consisting of 50.0 mmol L(-1) of acetic acid/sodium acetate buffer, 12.5 mmol L(-1) of CuSO(4), and standard and sample solutions prepared in 2.00 mmol L(-1) of Brij 35 and 12.5 mmol L(-1) of CuSO(4) were optimized. After evaluating validation parameters, the method was successfully applied to the analysis of samples in the form of tablets and sachets.
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Cidade Universitária CEP 36036-330, Juiz de Fora, MG, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Faria
authors.forename: Marcus V N
authors.initials: MV
authors.lastname: de Souza
authors.forename: Roy E
authors.initials: RE
authors.lastname: Bruns
authors.forename: Marcone A L
authors.initials: MA
authors.lastname: de Oliveira
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods* / Q000592:standards; D013997:Time Factors
chemical_list: D000995:Antitubercular Agents; D004573:Electrolytes