title: Carbadox-induced inhibition of aldosterone production in porcine adrenals in vitro.
abstract: Carbadox, a growth promoter used in pig husbandry, inhibited aldosterone production in sliced porcine adrenals in vitro. The adrenal slices, obtained from 3-5-wk-old piglets, were maintained in a Krebs solution and exposed to 1-40 mug carbadox/ml for 1 hr. Aldosterone was estimated (using a solid-phase radioimmunoassay kit) in samples taken hourly for up to 5 hr after carbadox exposure. The results showed a dose-dependent inhibition of aldosterone production by carbadox, with 25-35% inhibition by a concentration of 40 mug/ml. The findings support the hypothesis, based on clinical and histopathological evidence from in vivo experiments in pigs, that carbadox interferes with aldosterone production in the adrenal zona glomerulosa.
authors.affiliation: Central Veterinary Institute, Edelhertweg 15, 8219 PH Lelystad, The Netherlands.
authors.forename: T J
authors.initials: TJ
authors.lastname: Spierenburg
authors.forename: A J
authors.initials: AJ
authors.lastname: Baars
authors.forename: G J
authors.initials: GJ
authors.lastname: de Graaf
authors.forename: L P
authors.initials: LP
authors.lastname: Jager