[Isolation of a bacterial strain capable of bezafibrate-degrading and biodegradation characteristics].
OBJECTIVE
Bezafibrate is one of the most frequently detected pharmaceuticals at relatively high concentration in surface water and even in drinking water. Biodegradation is an important way to solve the problem. This study aimed to isolate, identify and characterize a bezafibrate-degrading bacterium.


METHODS
Strain B31 capable of degrading bezafibrate by cometabolism was isolated from activated sludge of sewage treatment plant in Shanghai, China, and identified based on its morphology, physiology and phylogenetic analysis of 16S rRNA sequence. To evaluate the ability of degradation, the concentration of bezafibrate was detected by high performance liquid chromatography.


RESULTS
Strain B31 was identified to be closely related to Pseudomonas putida. The optimum condition of degrading bezafibrate was at 30 degrees C, pH 7. After 5 days, Strain B31 could degrade 30 mg/L bezafibrate by 48% in liquid mineral salt medium with 1% methanol as primary substrate. And the rate of degradation could enhance to 61%, 72.6%, 76.67%, when 5 g/L glucose, peptone and yeast extract as primary substrate, respectively.


CONCLUSIONS
The strain has the potential for bezafibrate biodegradation.
Yang
Xing
X
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China. yangxinwww@163.com
Xue
Gang
G
Zhao
Xiaoxiang
X
Liu
Yanan
Y
D001629:Bezafibrate / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D000960:Hypolipidemic Agents / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D000960:Hypolipidemic Agents; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D001629:Bezafibrate