Determination of total sulfite in wine by ion chromatography after in-sample oxidation.
Sulfur dioxide (SO2) or sulfites are the most common preservatives used in winemaking. The level of total SO2 is subject to regulation. Currently, the regulatory determination of total SO2 (including sulfites) is done by the optimized Monier-Williams (OMW) method, which includes time-consuming distillation and titration steps. This paper describes the development and application of an alternative, rapid, straightforward, and reliable method for the determination of total sulfite in wine. In this method, a simple oxidation step using alkaline hydrogen peroxide (H2O2) solution is followed by ion chromatographic (IC) analysis of sulfate coupled with conductometric detection. Thirteen wines were analyzed in order to compare the in-sample oxidation method with the OMW-procedure. A t-test revealed satisfying compliance regarding sample preparation, i.e., alkaline H2O2 treatment and acidic distillation (OMW method). Comparable results were also obtained between IC analysis and acid/base titration. Our results indicate that the novel method (limit of quantification: 4 mg SO2 L(-1)) is well suited for the cost-efficient monitoring of regulatory limits.
Koch
Matthias
M
BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Strasse 11, D-12489 Berlin, Germany. matthias.koch@bam.de
Köppen
Robert
R
Siegel
David
D
Witt
Angelika
A
Nehls
Irene
I
D002853:Chromatography, Liquid / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D010084:Oxidation-Reduction; D013447:Sulfites / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D013447:Sulfites; D006861:Hydrogen Peroxide