title: Tetraodon nigroviridis as a nonlethal model of infectious spleen and kidney necrosis virus (ISKNV) infection.
abstract: Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus, family Iridoviridae. We have previously established a high mortality ISKNV infection model of zebrafish (Danio rerio). In this study, a nonlethal Tetraodon nigroviridis model of ISKNV infection was established. ISKNV infection did not cause lethal disease in Tetraodon but could infect almost all the organs of this species. Electron microscopy showed ISKNV particles were present in infected tissues. Immunofluorescence and quantitative real-time PCR analysis showed that nearly all the virions and infected cells were cleared at 14 d postinfection. The expression profiles of interferon-γ and tumor necrosis factor-α gene in response to ISKNV infection were significantly different in Tetraodon and zebrafish. The establishment of the nonlethal Tetraodon model of ISKNV infection can offer a valuable tool complementary to the zebrafish infection model for studying megalocytivirus disease, fish immune systems, and viral tropism.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen (Zhongshan) University, Guangzhou, PR China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xu
authors.forename: Lichao
authors.initials: L
authors.lastname: Huang
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Lin
authors.forename: Junliang
authors.initials: J
authors.lastname: Tang
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Li
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Lu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xia
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Yu
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D004195:Disease Models, Animal; D005393:Fish Diseases / Q000276:immunology / Q000401:mortality / Q000821:virology*; D007371:Interferon-gamma / Q000276:immunology; D007497:Iridoviridae / Q000235:genetics / Q000502:physiology*; D023721:Tetraodontiformes / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015027:Zebrafish / Q000276:immunology / Q000821:virology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma