title: Interaction between dietary conjugated linoleic acid and calcium supplementation affecting bone and fat mass.
abstract: Dietary conjugated linoleic acid (CLA) has shown wide biologically beneficial effects, such as anticancer, antiatherosclerotic, antidiabetic, immunomodulating, and antiobesity effects. However, the effects of CLA on total body ash, reflective of bone mineral content, have not been consistent. We hypothesized that the inconsistency of the CLA effect on ash may be linked to interaction between CLA and dietary calcium levels. Thus, we investigated the effects of CLA on body ash in conjunction with various calcium levels. Male ICR mice were fed three different levels of calcium (0.01, 0.5, and 1%) with or without 0.5% CLA for 4 weeks for Experiment 1 and separate CLA isomers at 0.22% level with 1% calcium in Experiment 2. CLA feeding reduced body fat regardless of dietary calcium level, whereas CLA supplementation increased body ash compared to control only in animals fed the 1% calcium. In Experiment 2 it was confirmed that this observation was associated with the trans-10, cis-12 CLA isomer, but not with the cis-9, trans-11 isomer. CLA administration with 1% dietary calcium significantly improved total ash percent (%) in femurs, confirming that CLA has the potential to be used to improve bone mass.
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, 100 Holdsworth Way, Amherst, MA 01003, USA.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.forename: Michael
authors.initials: M
authors.lastname: Terk
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Park
mesh_terms: D050154:Adiposity / Q000187:drug effects*; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D002136:Calcium, Dietary / Q000008:administration & dosage / Q000494:pharmacology*; D019587:Dietary Supplements; D005247:Feeding Behavior / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D009928:Organ Specificity / Q000187:drug effects
chemical_list: D002136:Calcium, Dietary; D044243:Linoleic Acids, Conjugated; D002118:Calcium