Evaluation of the effect of IL-22 on human cord blood CD4+ T cells.
IL-22 is a member of IL-10 cytokine family which is believed to play an important role in inflammatory responses. IL-22 has similarities with IL-10 including conserved sequences with IL-10. IL-22 receptor is also comprised of two chains known as L-22R1 and L-10R2; supporting the speculation that the two cytokines may have similar effects. The aim of this study was to shed some light on the biological activity of IL-22 upon the cord blood CD4+CD25- T cells. In this research, cord blood T CD4+CD25- cells were cultured in presence of anti CD2/CD3/CD28 coated beads, IL-2 and IL-22 for two weeks at 37 degrees C and 5% CO2. Flow cytometry analysis showed that IL-22 has no effect upon CD25 and Foxp3 expression. Also, the results indicated that IL-22 is not involved in CD4+ T cell proliferation. Moreover, the results of suppression assay did not show any suppression effect on the cultured T cells. Thus, it seems that umbilical cord blood T cells probably do not express IL-22R1 on their surface.
Arasteh
Javad
J
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Pourpak
Zahra
Z
Ebtekar
Masoumeh
M
Pourfathollah
Ali Akbar
AA
Hassan
Zuhair Mohammad
ZM
Farahmandian
Tahereh
T
Niknam
Hamid Mahmoudzadeh
HM
D018801:CD2 Antigens / Q000097:blood; D018106:CD28 Antigens / Q000097:blood; D017252:CD3 Complex / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D051858:Forkhead Transcription Factors / Q000097:blood; D006801:Humans; D007378:Interleukins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D000097043:Interleukin-22
D018801:CD2 Antigens; D018106:CD28 Antigens; D017252:CD3 Complex; D051858:Forkhead Transcription Factors; D007378:Interleukins; D000097043:Interleukin-22; C418974:FOXP3 protein, human