Analysis of omnoponum by surface-ionization mass spectrometry and liquid chromatography tandem mass spectrometry methods.
This paper provides the development of analytical capabilities of surface-ionization mass spectrometry (SI/MS) and high performance liquid chromatography with tandem mass spectrometry (HPLC/MS/MS) for narcotic analgesic omnoponum, which perfectly exemplifies a mixture of opium alkaloids. It has been revealed that the investigated opiates solution, omnoponum, is ionized by the surface ionization (SI) method with high sensitivity. In the SI mass spectrum, M+, (M-H)+, (M-H-2nH)+, (M-R)+ and (M-R-2nH)+ ion lines, where M is a molecule, H is the hydrogen atom and R is a radical, were observed. These ion lines consist of combined omnoponum mixture SI mass spectra, i.e. morphine, codeine, thebaine, papaverine, and narcotine. Moreover, while the study of omnoponum by HPLC/MS/MS methods has attested that the mixture really consists of 5 components, it has been demonstrated that the SI/MS method can be utilized for the analysis of this mixture without the necessity of its chromatographic separation.
Usmanov
Dilshadbek
D
Arifov Institute of Electronics, Dormon Yoli Street 33, Akademgorodok, Tashkent, Uzbekistan. usmanov@aie.uz
Khasanov
Usman
U
Pantsirev
Aleksey
A
Van Bocxlaer
Jan
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000032:analysis; D009020:Morphine / Q000032:analysis; D009892:Opium / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D009020:Morphine; D009892:Opium; D003061:Codeine