title: In vitro evaluation of marine-microorganism extracts for anti-viral activity.
abstract: Viral-induced infectious diseases represent a major health threat and their control remains an unachieved goal, due in part to the limited availability of effective anti-viral drugs and measures. The use of natural products in drug manufacturing is an ancient and well-established practice. Marine organisms are known producers of pharmacological and anti-viral agents. In this study, a total of 20 extracts from marine microorganisms were evaluated for their antiviral activity. These extracts were tested against two mammalian viruses, herpes simplex virus (HSV-1) and vesicular stomatitis virus (VSV), using Vero cells as the cell culture system, and two marine virus counterparts, channel catfish virus (CCV) and snakehead rhabdovirus (SHRV), in their respective cell cultures (CCO and EPC). Evaluation of these extracts demonstrated that some possess antiviral potential. In sum, extracts 162M(4), 258M(1), 298M(4), 313(2), 331M(2), 367M(1) and 397(1) appear to be effective broad-spectrum antivirals with potential uses as prophylactic agents to prevent infection, as evident by their highly inhibitive effects against both virus types. Extract 313(2) shows the most potential in that it showed significantly high inhibition across all tested viruses. The samples tested in this study were crude extracts; therefore the development of antiviral application of the few potential extracts is dependent on future studies focused on the isolation of the active elements contained in these extracts.
authors.affiliation: Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A, Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA.
authors.forename: Jarred
authors.initials: J
authors.lastname: Yasuhara-Bell
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Yang
authors.forename: Russell
authors.initials: R
authors.lastname: Barlow
authors.forename: Hank
authors.initials: H
authors.lastname: Trapido-Rosenthal
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000737:chemistry* / Q000302:isolation & purification; D002460:Cell Line; D017377:Diatoms / Q000737:chemistry* / Q000302:isolation & purification; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D052656:Ictalurivirus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D029141:Novirhabdovirus / Q000187:drug effects; D012623:Seawater / Q000382:microbiology*; D018116:Vesiculovirus / Q000187:drug effects; D014780:Viruses / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents