title: Polymer particle shape independently influences binding and internalization by macrophages.
abstract: The interaction of macrophages with micro and nanoparticles (MNPs) is important because these cells clear particles from the circulation, and because they are potential therapeutic targets in inflammatory conditions, atherosclerosis and cancer. Therefore, an understanding of the features of MNPs that influence their interaction with macrophages may allow optimization of their properties for enhanced drug delivery. In this study, we show that particle shape impacts phagocytosis by macrophages, and more importantly, that particle shape and size separately impact attachment and internalization. The study provides a methodology for further exploring how particle shape can be controlled to achieve desired attachment and internalization. The results of the study also give mechanistic guidance on how particle shape can be manipulated to design drug carriers to evade macrophages, or alternatively to target macrophages.
authors.affiliation: Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Sharma
authors.forename: David T
authors.initials: DT
authors.lastname: Valenta
authors.forename: Yoav
authors.initials: Y
authors.lastname: Altman
authors.forename: Sheryl
authors.initials: S
authors.lastname: Harvey
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Samir
authors.initials: S
authors.lastname: Mitragotri
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Smith
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004337:Drug Carriers; D005434:Flow Cytometry; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles; D010316:Particle Size; D010587:Phagocytosis; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D013678:Technology, Pharmaceutical; D013997:Time Factors
chemical_list: D004337:Drug Carriers; D011137:Polystyrenes