title: A distinct subset of podoplanin (gp38) expressing F4/80+ macrophages mediate phagocytosis and are induced following zymosan peritonitis.
abstract: Macrophages are important tissue resident cells that regulate the dynamics of inflammation. However, they are strikingly heterogeneous. During studies looking at podoplanin (gp38) expression on stromal cells in the murine spleen and peritoneal cavity we unexpectedly discovered that podoplanin was expressed on a subset of F4/80(+) macrophages; a subset which we have termed fibroblastic macrophages (FM). These cells function as phagocytes in vitro as measured by bead mediated phagocytosis assays. FM also exist at high frequency in the peritoneal cavity and in zymosan induced peritonitis in vivo. These FM represent a unique subgroup of F4/80(+) macrophages and their presence in the inflamed peritoneum suggests that they play a role in zymosan induced peritonitis.
authors.affiliation: School of Immunity and Infection, MRC Centre for Immune Regulation, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.
authors.forename: Tie Zheng
authors.initials: TZ
authors.lastname: Hou
authors.forename: Jonas
authors.initials: J
authors.lastname: Bystrom
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Sherlock
authors.forename: Omar
authors.initials: O
authors.lastname: Qureshi
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Parnell
authors.forename: Graham
authors.initials: G
authors.lastname: Anderson
authors.forename: Derek W
authors.initials: DW
authors.lastname: Gilroy
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Buckley
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology*; D010587:Phagocytosis; D015054:Zymosan / Q000494:pharmacology; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D015054:Zymosan; C490441:Pdpn protein, mouse; D000098853:Podoplanin