Generation of chimeric T-cell receptor transgenes and their efficient transfer in primary mouse T lymphocytes.
Gene modification of T cells with chimeric T-cell receptor (TCR) transgenes offers a novel way to generate tumor-specific T cells for cancer immunotherapy. Retroviruses have been utilized as the most common means of efficiently transducing primary T lymphocytes with these transgenes. In this section we describe methods for generation of chimeric TCR's and utilization of retroviral vectors for efficient transduction of these transgenes in primary mouse T lymphocytes.
Howland
Linda J
LJ
Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Australia.
Haynes
Nicole M
NM
Darcy
Phillip K
PK
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes / Q000235:genetics*
D016207:Cytokines; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins