title: Estrogenic effects of o,p'-DDT exposure in Japanese medaka (Oryzias latipes).
abstract: The persistent pesticide 1,1,1-trichloro-2-(2-chlorophenyl)-2-(4-chlorophenyl)ethane (o,p'-DDT) has been implicated as an endocrine-disrupting chemical. In this study, we performed DNA microarray analysis to assess hepatic gene expression in male Japanese medaka (Oryzias latipes) exposed to 1 ppb and 100 ppb o,p'-DDT for 48 hr. Results showed that 1 ppb o,p'-DDT induced the expression of choriogenins (chgH, chgL, and chgH minor), and 100 ppb induced the expression of vitellogenins (vtgI and vtgII) and estrogen receptor alpha. These genes showed considerably high up-regulation among the genes assayed and showed good dose-dependency. Thus, in this study the female hormone-like endocrine-disrupting effect of o,p'-DDT at gene expression level was clearly observed in Japanese medaka.
authors.affiliation: Graduate School of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto, Japan. uchida@ecogenomics.co.jp
authors.forename: Masaya
authors.initials: M
authors.lastname: Uchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kagami
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Kusano
authors.forename: Koji
authors.initials: K
authors.lastname: Arizono
mesh_terms: D000818:Animals; D003634:DDT / Q000633:toxicity*; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D009990:Oryzias / Q000235:genetics; D010575:Pesticides / Q000633:toxicity*
chemical_list: D047628:Estrogen Receptor alpha; D004968:Estrogens, Non-Steroidal; D010575:Pesticides; D004958:Estradiol; D003634:DDT; C016340:o,p'-DDT