Adsorption and bioactivity of tyrosine hydroxylase on gold surfaces and nanoparticles.
Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.
Halskau
Oyvind
O
Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway. Oyvind.Halskau@biomed.uib.no
Volden
Sondre
S
Calvo
Ana C
AC
Martínez
Aurora
A
Glomm
Wilhelm R
WR
D000327:Adsorption; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020033:Protein Isoforms; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry* / Q000378:metabolism
D004800:Enzymes, Immobilized; D020033:Protein Isoforms; D011994:Recombinant Proteins; D006046:Gold; D014446:Tyrosine 3-Monooxygenase