title: Distinct granuloma responses in C57BL/6J and BALB/cByJ mice in response to pristane.
abstract: Granuloma formation is an inflammatory response of the host against invading pathogens or indigestible substances. We generated mesenteric oil granulomas by injecting pristane into the peritoneal cavity (PC) of mice, and compared oil granuloma formation in the C57BL/6J and BALB/cByJ strains of mice. The formation and kinetics of oil granulomas were distinct between the two strains. In C57BL/6J mice, injected pristane induced oil granuloma formation at both the mesenteric centers (MG) and margins (SG). MG was resolving by 11 weeks, and SG persisted. In BALB/cByJ mice, MG developed slower but persisted longer than in C57BL/6J mice, and SG resolved sooner than in C57BL/6J mice. Injection of India ink revealed that phagocytes were localised mainly to the SG in C57BL/6J mice, but were located diffusely in both MG and SG of BALB/cByJ mice. SG cells expressed more monocyte chemotactic protein-1 (MCP-1) mRNA than MG cells in C57BL/6J mice, but there was no difference in MCP-1 expression between the MG and SG in BALB/cByJ mice. These observations suggest that the recruitment of inflammatory leucocytes under the direction of chemokines differentiates the patterns of granuloma responses to pristane in C57BL/6J and BALB/cByJ mice.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC, USA.
authors.forename: Huaiyong
authors.initials: H
authors.lastname: Chen
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liao
authors.forename: Derek
authors.initials: D
authors.lastname: Cain
authors.forename: Ian
authors.initials: I
authors.lastname: McLeod
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ueda
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Guan
authors.forename: Christian
authors.initials: C
authors.lastname: Raetz
authors.forename: Garnett
authors.initials: G
authors.lastname: Kelsoe
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006099:Granuloma / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008297:Male; D008643:Mesentery / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008810:Mice, Inbred C57BL / Q000276:immunology*; D010537:Peritoneum / Q000276:immunology / Q000473:pathology; D010586:Phagocytes / Q000276:immunology / Q000473:pathology; D013045:Species Specificity; D013729:Terpenes / Q000633:toxicity*
chemical_list: D007166:Immunosuppressive Agents; D013729:Terpenes; C009042:pristane