title: Colorectal cancer and genetic polymorphism of DNA double-strand break repair gene XRCC4 in Taiwan.
abstract: The DNA repair gene X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4) is thought to play a major role in the caretaking of the whole genome via double-strand break repair. However, the association of polymorphic variants of XRCC4 with colorectal cancer susceptibility has never been reported. In this hospital-based case-control study, the association of XRCC4 polymorphisms C-1622T (rs7727691), G-1394T (rs6869366), G-652T (rs2075685), C-571T (rs2075686), intron 3 DIP (rs28360071), S247A (rs3734091) and intron 7 DIP (rs28360317) with colorectal cancer risk in a Taiwanese population was investigated. The genotypes of XRCC4 of 370 patients with colorectal cancer and 370 age- and gender-matched healthy controls were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. We found significant differences in the genetic and allelic frequencies of the XRCC4 G-1394T between the colorectal cancer and control groups (p=0.0003 and 8.32 x 10(-5), respectively). The distributions of other genetic polymorphisms between cases and the control group were not significantly different. We conclude that the G allele of XRCC4 G-1394T may contribute to colorectal carcinogenesis and may be useful for early detection of colorectal cancer.
authors.affiliation: Terry Fox Cancer Research Lab, China Medical University Hospital, 2 Yuh-Der Road, Taichung, 404 Taiwan, R.O.C. datian@mail.cmuh.org.tw
authors.forename: Da-Tian
authors.initials: DT
authors.lastname: Bau
authors.forename: Mei-Due
authors.initials: MD
authors.lastname: Yang
authors.forename: Yung-An
authors.initials: YA
authors.lastname: Tsou
authors.forename: Song-Shei
authors.initials: SS
authors.lastname: Lin
authors.forename: Cheng-Nan
authors.initials: CN
authors.lastname: Wu
authors.forename: Hao-Hsueh
authors.initials: HH
authors.lastname: Hsieh
authors.forename: Rou-Fen
authors.initials: RF
authors.lastname: Wang
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsai
authors.forename: Wen-Shin
authors.initials: WS
authors.lastname: Chang
authors.forename: Hsiu-Min
authors.initials: HM
authors.lastname: Hsieh
authors.forename: Shung-Shung
authors.initials: SS
authors.lastname: Sun
authors.forename: Ru-Yin
authors.initials: RY
authors.lastname: Tsai
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007438:Introns; D011110:Polymorphism, Genetic; D013624:Taiwan
chemical_list: D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human