[Effect of thiolactone homocysteine loading on adenosine metabolism in rats: relationship with platelet hyper-reactivity, correction of this metabolism disorders by vitamin-microelement complex].
Influence of DL-homocysteine thiolactone loading (100 mg/kg by intragastric administration for 28 days) on enzymes activity of adenylic nucleotide and adenosine metabolism in the blood serum, platelets and liver of rats was investigated. The relation between revealed disturbance and platelet hyper-reactivity was estimated. It was established, that apyrase and 5'-nucleotidase activities decreased and adenosine deaminase activity increased in platelets of the rats with hyperhomocysteinemia (HHC). HHC also interrupted adenosine production in the blood serum and liver in rats. Under this condition the platelet sensitivity to ADP-stimulation was significantly increased. Vitamin-microelement complex decreased HHC-induced disorder of adenosine metabolism and prevented platelet hyper-reactivity formation. In vitro homocysteine inhibited platelet hydrolysis of ADP and AMP in a dose-dependent manner and this effect reduced in the presence of hydrogen sulfide donor NaHS.
Zaichko
N V
NV
D015720:5'-Nucleotidase / Q000097:blood / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D001081:Apyrase / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D004338:Drug Combinations; D006710:Homocysteine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D020138:Hyperhomocysteinemia / Q000097:blood / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D015539:Platelet Activation / Q000187:drug effects*; D051381:Rats; D014131:Trace Elements / Q000008:administration & dosage / Q000627:therapeutic use*; D016896:Treatment Outcome; D014815:Vitamins / Q000008:administration & dosage / Q000627:therapeutic use*
D004338:Drug Combinations; D014131:Trace Elements; D014815:Vitamins; D006710:Homocysteine; C007957:homocysteine thiolactone; D015720:5'-Nucleotidase; D001081:Apyrase; D000241:Adenosine