Nuclear copies of mitochondrial genes: another problem for ancient DNA.
The application of ancient DNA techniques is subject to many problems caused primarily by low quality and by low quantity of DNA. For these reasons most studies employing ancient DNA rely on the characterization of mitochondrial DNA, which is present in many more copies per cell than nuclear DNA and hence more copies are likely to survive. We used universal and taxon specific mitochondrial primers to amplify DNA from museum specimens, and found many instances where the amplification of nuclear copies of the mitochondrial gene (numts) instead of the targeted mitochondrial fragment had occurred. Furthermore, the likelihood of amplifying numts increased dramatically when universal primers were utilized. Here we suggest that ancient DNA practitioners must consider the possibility that numts can be amplified at higher rates than previously thought. This is another complication for ancient DNA studies, but it also suggests that more extensive inclusion of nuclear markers in ancient DNA studies should be feasible.
den Tex
Robert-Jan
RJ
Department of Evolutionary Biology, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, Sweden.
Maldonado
Jesus E
JE
Thorington
Richard
R
Leonard
Jennifer A
JA
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial / Q000235:genetics*; D005784:Gene Amplification; D050259:Genes, Mitochondrial; D012589:Sciuridae / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial