title: [Contributions of putative lipoate-protein ligase to the virulence of Streptococcus pneumoniae].
abstract: OBJECTIVE
  To investigate the effect of putative lipoate-protein ligase (LPL) on the virulence of Streptococcus pneumoniae.
  
  
  METHODS
  lpl gene deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The cell adherence assay and mice challenge assay were used to observe the differences between wild strain and the mutant in the pathopoiesis of Streptococcus pneumoniae.
  
  
  RESULTS
  Mice virulence experiments showed that the median lethal time of wide type and the lpl mutant are both 12 h, no statistics difference. The ability of adherence of the mutant was greater than the wild strain (P < 0.01); The capsule stain in-vivo showed that the wild strain and the mutant both had the capsule.
  
  
  CONCLUSIONS
  lpl gene inhibits the adherence to host, but no affect on the ability to infection mice by
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 400016, China. yangxiaoliang2010@yeah.net
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Yang
authors.forename: Nanlin
authors.initials: N
authors.lastname: Yin
authors.forename: Dan
authors.initials: D
authors.lastname: Pang
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Wu
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D011008:Pneumococcal Infections / Q000382:microbiology* / Q000401:mortality; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D010453:Peptide Synthases; C072322:lipoate-protein ligase