Microarray analysis of differentially expressed genes in vaginal tissues in postmenopausal women. The role of stress urinary incontinence.
OBJECTIVE
This study aimed to explore gene expression and their regulatory mechanisms in etiology of stress urinary incontinence (SUI).


METHODS
Tissue samples were taken from the paraurethral vaginal wall from three pairs of postmenopausal, age/body mass index/parity-matched SUI, and continent women and subjected to microarray analysis using the Affymetrix GeneChip® Human Genome U133 Plus 2.0 oligonucleotide chip set. The significantly differentially expressed genes and their protein expression were confirmed by quantitative PCR and Western blot.


RESULTS
Seventy-five differentially expressed genes were listed; 31 genes were upregulated in SUI group, while 44 were downregulated. Gene metabolic pathway analysis showed that solutable N-ethylmaleimide-sensitive factor attachment protein receptor signaling pathway and neurodegenerative disorder pathway were the most predominant.


CONCLUSIONS
Apolipoprotein E, growth factor receptor-bound protein 2, Golgi SNAP receptor complex member 1, and glucosidase, beta, acid may play a neurodegenerative role in SUI development.
Tong
Jiali
J
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. jialit97@163.com
Lang
Jinghe
J
Zhu
Lan
L
D000368:Aged; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D051380:GRB2 Adaptor Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046228:Microarray Analysis; D008875:Middle Aged; D017698:Postmenopause; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014550:Urinary Incontinence, Stress / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism*; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
D001057:Apolipoproteins E; C490972:GOSR1 protein, human; D051380:GRB2 Adaptor Protein; D050766:Qb-SNARE Proteins; D001617:beta-Glucosidase