[Cloning and expression of the osmotic tolerance gene Bbmpd of Beauveria bassiana].
OBJECTIVE
We isolate the gene involved in osmotolerance of Beauveria bassiana for investigation the adaptation mechanism of the fungus to adversity.


METHODS
T-DNA tagging and genomic DNA walking were carried out by using Y-shaped adaptor dependent extension (YADE) method. RT-PCR was used to analyze the transcription profile of Bbmpd.


RESULTS
A gene (Bbmpd) encoding mannitol 1-phosphate dehydrogenase in B. bassiana was identified. The transcription of Bbmpd was induced by osmostress (0.8 mol/L NaCl) in wild type strain, while significantly decreased in Bbhog1 mutant, indicating that the transcription of Bbmpd was regulated by Hog1 pathway in B. bassiana. Bbmpd-disrupted mutants showed sensitive to osmostress on Czapek's medium containing 0.8 mol/L NaC1. However, the growth and sporulation of the Bbmpd-disrupted mutants in vitro were not significantly different from that of wild type strain.


CONCLUSIONS
Mannitol 1-phosphate dehydrogenase gene was isolated by T-DNA tagging,and was found to be involved in the osmostress of B. bassiana. The transcription of Bbmpd was induced by osmostress and regulated by Hog1 pathway.
Luo
Zhibing
Z
Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture of China, Biotechnology Research Center,Southwest University, Chongqing 400716, China. luozb1@163.com
Jin
Kai
K
Zhang
Yongjun
Y
Wu
Zengqiang
Z
Pei
Yan
Y
D052982:Beauveria / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D009995:Osmosis; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; D013401:Sugar Alcohol Dehydrogenases; C021544:mannitol-1-phosphate dehydrogenase