title: A novel basalt fiber-reinforced polylactic acid composite for hard tissue repair.
abstract: A basalt fiber (BF) was, for the first time, introduced into a poly(l-lactic acid) (PLLA) matrix as innovative reinforcement to fabricate composite materials for hard tissue repair. Firstly, BF/PLLA composites and pure PLLA were produced by the methods of solution blending and freeze drying. The results showed that basalt fibers can be uniformly dispersed in the PLLA matrix and significantly improve the mechanical properties and hydrophilicity of the PLLA matrix. The presence of basalt fibers may retard the polymer degradation rate and neutralize the acid degradation from PLLA. Osteoblasts were cultured in vitro to evaluate the cytocompatibility of the composite. An MTT assay revealed that osteoblasts proliferated well for 7 days and there was little difference found in their viability on both PLLA and BF/PLLA films, which was consistent with the alkaline phosphatase (ALP) activity results. A fluorescent staining observation showed that osteoblasts grew well on the composites. SEM images displayed that osteoblasts tended to grow along the fiber axis. The formation of mineralized nodules was observed on the films by Alizarin red S staining. These results suggest that the presence of basalt fibers does not noticeably affect osteoblastic behavior and the designed composites are osteoblast compatible. It is concluded that basalt fibers, as reinforcing fibers, may have promising applications in hard tissue repair.
authors.affiliation: Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D018786:Bone Substitutes / Q000138:chemical synthesis*; D019245:Compressive Strength; D055119:Elastic Modulus; D004867:Equipment Design; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D017640:Silicates / Q000138:chemical synthesis*; D013718:Tensile Strength; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D017640:Silicates; C060346:basalt; D019344:Lactic Acid; C033616:poly(lactide)