title: Ex vivo study on ATPase activities of rabbit renal proximal tubules as a predictive model for nephrotoxicity: Comparison with an in vitro study on a new cephalosporin (cefpirome).
abstract: An ex vivo study on adenosine triphosphatase (ATPase) activities of rabbit renal proximal tubules was conducted with a new cephalosporin, cefpirome (HR 810), a positive control, cephaloridine, and a reference third-generation cephalosporin, cefotaxime. Compared with controls, CPH caused a significant time-dependent decrease in ATPase activities [12%, 2 hr after treatment (P < 0.01) and 75%, 48 hr after treatment (P < 0.001)]. This decrease was accompanied by a significant loss in the energy charge of the adenylate pool [27%, 2 hr after treatment (P < 0.001)]. Neither cefotaxime nor cefpirome caused such decreases. The results confirmed those of a previously published in vitro study. The advantages and disadvantages of these two experimental procedures as predictive models for nephrotoxicity are discussed.
authors.affiliation: INSERM U 356, 75006 Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Dutertre-Catella
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.forename: M F
authors.initials: MF
authors.lastname: Olivier
authors.forename: M
authors.initials: M
authors.lastname: Thevenin
authors.forename: J M
authors.initials: JM
authors.lastname: Warnet
authors.forename: B
authors.initials: B
authors.lastname: Vannier
authors.forename: J R
authors.initials: JR
authors.lastname: Claude
authors.forename: R A
authors.initials: RA
authors.lastname: Podevins