Laser-improved protein crystallization screening.
Screening of proteins for crystallization under laser irradiation was investigated using six proteins: ribonuclease B, glucose dehydrogenase, lysozyme, sorbitol dehydrogenase, fructose dehydrogenase and myoglobin. Shining 532 nm green circularly polarized laser light with a picosecond pulse and 6 mW power for 30 s on newly set-up protein drops showed a marked improvement in the number of screen conditions amenable for crystal growth compared with control drops under identical conditions but without laser exposure. For glucose dehydrogenase and sorbitol dehydrogenase, larger and better quality crystals were formed and the resolution of X-ray diffraction was improved. The speed of crystallization increased in the case of ribonuclease B, lysozyme and sorbitol dehydrogenase. During laser irradiation, the amount of precipitation in the screened drops increased, indicating a transient decrease in protein solubility. At the optimized laser settings, there was no deleterious effect of the laser on crystal growth or on the protein. In the cases of ribonuclease B and lysozyme the crystal packing did not change owing to the laser exposure.
Yennawar
Neela
N
Huck Institutes of Life Sciences, Pennsylvania State University, University Park, PA 16802, USA. nhy1@psu.edu
Denev
Sava
S
Gopalan
Venkataraman
V
Yennawar
Hemant
H
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D007834:Lasers; D011506:Proteins / Q000032:analysis*
D011506:Proteins