title: [cDNA cloning, characterization and mRNA expression of a profilin from the swimming crab Portunus trituberculatus].
abstract: We isolated and characterized the profilin full-length cDNA from hemocytes of swimming crab Portunus trituberculatus. The profilin cDNA consists of 742 bp and the 375 bp open reading frame encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. The deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito Anopheles gambiae. The profilin mRNA was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. The higher expression of profilin mRNA is observed in crab challenged by the pathogenic bacterium Vibrio parahaemolyticus. These results suggest a potential role for profilin in pathogen host defense mechanisms.
authors.affiliation: Laboratory of Marine Biology Resources and Molecular Engineering, Ocean Science College, Zhejiang Ocean University, Zhoushan 316004, China. shenwangzs@163.com
authors.forename: Wang
authors.initials: W
authors.lastname: Shen
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.forename: Ge
authors.initials: G
authors.lastname: Shi
authors.forename: Ri-Xin
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D043143:Hepatopancreas; D008969:Molecular Sequence Data; D051304:Profilins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D051304:Profilins; D012333:RNA, Messenger