Terahertz imaging applied to cancer diagnosis.
We report on terahertz (THz) time-domain spectroscopy imaging of 10 microm thick histological sections. The sections are prepared according to standard pathological procedures and deposited on a quartz window for measurements in reflection geometry. Simultaneous acquisition of visible images enables registration of THz images and thus the use of digital pathology tools to investigate the links between the underlying cellular structure and specific THz information. An analytic model taking into account the polarization of the THz beam, its incidence angle, the beam shift between the reference and sample pulses as well as multiple reflections within the sample is employed to determine the frequency-dependent complex refractive index. Spectral images are produced through segmentation of the extracted refractive index data using clustering methods. Comparisons of visible and THz images demonstrate spectral differences not only between tumor and healthy tissues but also within tumors. Further visualization using principal component analysis suggests different mechanisms as to the origin of image contrast.
Brun
M-A
MA
Life Science Laboratory, Advanced Materials Laboratories, Sony Corporation, Tokyo Medical and Dental University, 1-5-45 Yushima Bunkyo-ku, Tokyo, 113-8510, Japan.
Formanek
F
F
Yasuda
A
A
Sekine
M
M
Ando
N
N
Eishii
Y
Y
D021441:Carcinoma, Pancreatic Ductal / Q000175:diagnosis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis; D016000:Cluster Analysis; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis; D008495:Medical Oncology / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000175:diagnosis; D025341:Principal Component Analysis; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D055068:Terahertz Spectroscopy / Q000379:methods*