title: Molecular breeding of tomato lines for mass production of miraculin in a plant factory.
abstract: A transgenic tomato line (56B, "Moneymaker") that expresses the miraculin gene driven by the CaMV 35S promoter was crossed with a dwarf tomato ("Micro-Tom") for the molecular breeding of cultivars that are suitable for miraculin production in a closed cultivation system. Plant size, miraculin accumulation, and self-pruning growth were used as selection indicators for F2 plants. Two lines were chosen for further analysis, bred to the F6 or F7 generation and cultivated in a closed cultivation system. In 56B and the two crossed lines, the concentrations of miraculin in the pericarp were 140, 367, and 343 microg/g FW, respectively. We also estimated that 26.2, 73.6, and 45.9 kg FW/m2 of tomatoes and 2.2, 16.6, and 9.8 mg/m2 of miraculin in the pericarp, respectively, could be harvested per year. These two crossed lines will be useful for the mass production of miraculin, especially in a closed cultivation system.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Kato
authors.forename: Riichiro
authors.initials: R
authors.lastname: Yoshida
authors.forename: Ayako
authors.initials: A
authors.lastname: Kikuzaki
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Hirai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuroda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hiwasa-Tanase
authors.forename: Kenichi
authors.initials: K
authors.lastname: Takane
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ezura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Mizoguchi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D030821:Plants, Genetically Modified
chemical_list: D006023:Glycoproteins