A two-pathway analysis of meiotic crossing over and gene conversion in Saccharomyces cerevisiae.
Several apparently paradoxical observations regarding meiotic crossing over and gene conversion are readily resolved in a framework that recognizes the existence of two recombination pathways that differ in mismatch repair, structures of intermediates, crossover interference, and the generation of noncrossovers. One manifestation of these differences is that simultaneous gene conversion on both sides of a recombination-initiating DNA double-strand break ("two-sidedness") characterizes only one of the two pathways and is promoted by mismatch repair. Data from previous work are analyzed quantitatively within this framework, and a molecular model for meiotic double-strand break repair based on the concept of sliding D-loops is offered as an efficient scheme for visualizing the salient results from studies of crossing over and gene conversion, the molecular structures of recombination intermediates, and the biochemical competencies of the proteins involved.
Stahl
Franklin W
FW
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA. fstahl@uoregon.edu
Foss
Henriette M
HM
D002874:Chromosome Mapping; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000235:genetics; D003434:Crossing Over, Genetic; D053903:DNA Breaks, Double-Stranded; D053843:DNA Mismatch Repair; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D005785:Gene Conversion; D008040:Genetic Linkage; D008540:Meiosis; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins