Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity.
We attempted to select a fraction of common carp, Cyprinus carpio spermatozoa that best survived a conventional freeze/thaw procedure, by centrifugation of frozen/thawed sperm through a Percoll gradient (45% and 90%). The proportion of motile spermatozoa (65.81 ± 5.19%), their velocity (77.58 ± 31.07 μm/sec), and membrane integrity (83.66 ± 4.38% intact) were significantly higher in separated sperm than in whole samples (motility 23.36 ± 2.98%, velocity 55.55 ± 19.03 μm/sec, and membrane integrity 57.92 ± 4.65%). Our results demonstrated that Percoll gradient centrifugation shows promise as a technique for selecting high quality cryopreserved fish spermatozoa, which could be useful for cryobiological research. Further studies are needed to evaluate the potentially higher fertilizing ability of the separated spermatozoa.
Li
Ping
P
University of South Bohemia, Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic. pingli06@yahoo.com
Dzyuba
Boris
B
Hulak
Martin
M
Rodina
Marek
M
Boryshpolets
Sergey
S
Li
Zhi-Hua
ZH
Linhart
Otomar
O
D000818:Animals; D002347:Carps; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient / Q000662:veterinary*; D015925:Cryopreservation / Q000662:veterinary*; D008297:Male; D011205:Povidone / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure
C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone