title: Inhibitory effects of cyclosporine a eye drops on symptoms in late phase and delayed-type reactions in allergic conjunctivitis models.
abstract: We investigated the efficacy of cyclosporine A (CyA) eye drops on ocular symptoms in late phase and delayed-type reactions in guinea pig allergic conjunctivitis models. An emulsion of ovalbumin (OVA) and Freund's complete adjuvant (FCA) was intraperitoneally injected into guinea pigs, and 15% OVA solution was applied topically to the eyes to elicit late phase reactions. Following the early phase reaction, increased scores for hyperemia, swelling, edema, and discharge were detected 6 h after antigen challenge, and CyA eye drops significantly inhibited the increase in scores for edema and discharge, the increase in the number of infiltrating inflammatory cells, and the percentage of eosinophils among polymorphonuclear leukocytes in conjunctival tissue. To induce delayed-type reactions, guinea pigs were sensitized by injecting FCA into the footpad, followed by injections of purified protein derivative into palpebral conjunctivae 24 d later. Increased scores for hyperemia, swelling, and discharge were detected 6 h after the induction of delayed-type allergy, and CyA eye drops significantly inhibited the increase in scores for hyperemia and swelling. In contrast, betamethasone sodium phosphate eye drops showed a tendency to inhibit the symptoms in both late phase and delayed-type reactions, or inflammatory cell infiltration in the late phase reaction, but the inhibition was not significant. These results suggest that CyA eye drops are useful for suppressing ocular symptoms in both late phase and delayed-type reactions in allergic conjunctivitis models.
authors.affiliation: Research & Development Center, Santen Pharmaceutical Co., Ltd., Ikoma, Nara, Japan. daisuke.shii@santen.co.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shii
authors.forename: Shizu
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Miwa
authors.initials: M
authors.lastname: Yoshimi
authors.forename: Osamu
authors.initials: O
authors.lastname: Katsuta
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oda
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D003233:Conjunctivitis, Allergic / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004804:Eosinophils / Q000473:pathology; D005620:Freund's Adjuvant / Q000276:immunology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007322:Instillation, Drug; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D009883:Ophthalmic Solutions; D010047:Ovalbumin / Q000276:immunology; D013997:Time Factors
chemical_list: D007166:Immunosuppressive Agents; D009883:Ophthalmic Solutions; D016572:Cyclosporine; D010047:Ovalbumin; D005620:Freund's Adjuvant