title: Two-dimensional gel electrophoresis in proteomics: Past, present and future.
abstract: Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist. The events that have led to the birth of proteomics are also recalled, ending with a description of the now well-known limitations of two-dimensional gels in proteomics. However, the often-underestimated advantages of two-dimensional gels are also underlined, leading to a description of how and when to use two-dimensional gels for the best in a proteomics approach. Taking support of these advantages (robustness, resolution, and ability to separate entire, intact proteins), possible future applications of this technique in proteomics are also mentioned.
authors.affiliation: CNRS UMR5092, Biochemistry and Biophysics of Integrated Systems, CEA Grenoble, iRTSV/BSBBSI, Grenoble, France. Thierry.Rabilloud@cea.fr
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
authors.forename: Mireille
authors.initials: M
authors.lastname: Chevallet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Luche
authors.forename: Cécile
authors.initials: C
authors.lastname: Lelong
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000266:history / Q000379:methods* / Q000639:trends; D049673:History, 20th Century; D049674:History, 21st Century; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000266:history / Q000379:methods* / Q000639:trends
chemical_list: D020543:Proteome