Apoptotic effect of quercetin on HT-29 colon cancer cells via the AMPK signaling pathway.
Activation of AMP-activated protein kinase (AMPK), a physiological cellular energy sensor, strongly suppresses cell proliferation in both nonmalignant and tumor cells. This study demonstrates the mechanism of quercetin-induced apoptosis in HT-29 colon cancer cells. Treatment of cells with quercetin significantly decreased cell viability in a dose-dependent manner. Notably, quercetin increased cell cycle arrest in the G1 phase and up-regulated apoptosis-related proteins, such as AMPK, p53, and p21, within 48 h. Furthermore, in vivo experiments showed that quercetin treatment resulted in a significant reduction in tumor volume over 6 weeks, and apoptosis-related protein induction by quercetin was significantly higher in the 100 mg/kg treated group compared to the control group. All of these results indicate that quercetin induces apoptosis via AMPK activation and p53-dependent apoptotic cell death in HT-29 colon cancer cells and that it may be a potential chemopreventive or therapeutic agent against HT-29 colon cancer.
Kim
Hyeong-Jin
HJ
Department of Companion and Laboratory Animal Science, Kongju National University, 340-702 Yesan, Republic of Korea.
Kim
Sang-Ki
SK
Kim
Byeong-Soo
BS
Lee
Seung-Ho
SH
Park
Young-Seok
YS
Park
Byung-Kwon
BK
Kim
So-Jung
SJ
Kim
Jin
J
Choi
Changsun
C
Kim
Jong-Suk
JS
Cho
Sung-Dae
SD
Jung
Ji-Won
JW
Roh
Kyong-Hwan
KH
Kang
Kyung-Sun
KS
Jung
Ji-Youn
JY
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000503:physiopathology*; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
D010936:Plant Extracts; D011794:Quercetin; D055372:AMP-Activated Protein Kinases