title: Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases.
abstract: CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.
authors.affiliation: Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London NW9 5EQ, UK. hiran.dhanji@hpa.org.uk
authors.forename: Hiran
authors.initials: H
authors.lastname: Dhanji
authors.forename: Michel
authors.initials: M
authors.lastname: Doumith
authors.forename: Olivier
authors.initials: O
authors.lastname: Clermont
authors.forename: Erick
authors.initials: E
authors.lastname: Denamur
authors.forename: Russell
authors.initials: R
authors.lastname: Hope
authors.forename: David M
authors.initials: DM
authors.lastname: Livermore
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D058872:Epidemics; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases