[A new monoclonal antibody selectively distributes on hepatocellular membrane].
OBJECTIVE
Screening monoclonal antibodies selectively distribute on hepatocellular membrane by hybridoma technology and exploring their relationship with liver diseases.


METHODS
Plasma membrane vesicles of rat hepatocytes were prepared using density gradient centrifugation and BALB/c mice were immunized with the membrane vesicles. Monoclonal antibodies were made with hybridoma technology. The immunizing valences and monoclonal antibodies were detected and screened by ELISA. Mh7 antigen was identified by immunoprecipitation method. Liver tissues of carbon tetrachloride injured rat models and diabetic rat models were used to detect the pathology value of mh7-antigen.


RESULTS
Hepatocellular membrane vesicles were obtained successfully. Several monoclonal antibodies were yielded by hybridoma technology. Immunofluorescence and pre-embedding immunogold-silver cytochemistry confirmed that mh7-antigen was a membrane molecule and with a 200KD molecular weight. Immunohistochemistry results indicated mh7 selectively distributed on hepatocellular membrane. Results with rat models demonstrated that mh7-antigen was dramatically reduced in fatty degenerated hepatocyte of carbon tetrachloride injured rat models and distributed as straps in diabetic rat models.


CONCLUSIONS
Mh7 is a new hepatocellular membrane monoclonal antibody and may closely related with liver diseases.
Cui
Li-na
LN
State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, the Fourth Military Medical University, Xi'an 710032, China.
Wang
Jing-bo
JB
Qiao
Li-juan
LJ
Wang
Xue-chang
XC
Zhou
Xin-min
XM
Han
Ying
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology*; D022781:Hepatocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal