Suppressed phosphorylation of collapsin response mediator protein-2 in the hippocampus of HCNP precursor transgenic mice.
We previously reported a novel peptide, Hippocampal Cholinergic Neurostimulating Peptide (HCNP), which induces acetylcholine synthesis by increasing the amount of choline acetyltransferase (ChAT) in medial septal nuclei. The HCNP precursor protein (HCNP-pp), composed of 186 amino acids, is an inhibitory factor of the c-Raf/MEK cascade and may be involved in fetal rat brain development via the inhibition of phosphorylation of Erk. To clarify the involvement of HCNP in hippocampal cholinergic circuitry, we previously generated HCNP-pp transgenic (HCNP-pp Tg) mice using the promoter of the α subunit of Ca(2+) calmodulin-dependent protein kinase II (CaMKIIα). These mice showed increased levels of ChAT in medial septal nuclei at 12 weeks of age, and the phenotype of depressive mood at 30 weeks of age. Here, through proteomic analysis we investigated the alteration of protein expression in the hippocampus of HCNP-pp Tg mice compared with wild-type littermate mice. We demonstrate that the activation of collapsin response mediator protein-2 (CRMP-2) is increased in the transgenic mice at 12 weeks of age when compared with wild-type littermate mice.
Kanamori
Tetsuko
T
Department of Neurology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Matsukawa
Noriyuki
N
Kobayashi
Hatasu
H
Uematsu
Norihiko
N
Sagisaka
Takafumi
T
Toyoda
Takanari
T
Kato
Daisuke
D
Oikawa
Shinji
S
Ojika
Kosei
K
D000367:Age Factors; D000818:Animals; D015536:Down-Regulation / Q000235:genetics*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015854:Up-Regulation / Q000235:genetics
D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D049488:Phosphatidylethanolamine Binding Protein; D011498:Protein Precursors; C095114:collapsin response mediator protein-2; D048049:Extracellular Signal-Regulated MAP Kinases