H2O2-sensitive quantum dots for the label-free detection of glucose.
A novel label-free detection system based on CdTe/CdS quantum dots (QDs) was designed for the direct measurement of glucose. Herein we demonstrated that the photoluminescence (PL) of CdTe/CdS QDs was sensitive to hydrogen peroxide (H(2)O(2)). With d-glucose as a substrate, H(2)O(2) that intensively quenched the QDs PL can be produced via the catalysis of glucose oxidase (GOx). Experimental results showed that the decrease of the QDs PL was proportional to the concentration of glucose within the range of 1.8 microM to 1mM with the detection limit of 1.8 microM under the optimized experimental conditions. In addition, the QD-based label-free glucose sensing platform was adapted to 96-well plates for fluorescent assay, enhancing the capabilities and conveniences of this detection platform. An excellent response to the concentrations of glucose was found within the range of 2-30 mM. Glucose in blood and urine samples was effectively detected via this strategy. The comparison with commercialized glucose meter indicated that this proposed glucose assay system is not only simple, sensitive, but also reliable and suitable for practical application. The high sensitivity, versatility, portability, high-throughput and low cost of this glucose sensor implied its potential in point-of-care clinical diagnose of diabetes and other fields.
Hu
Mei
M
Laboratory of Advanced Materials, and Department of Materials Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Tian
Jing
J
Lu
Hao-Ting
HT
Weng
Li-Xing
LX
Wang
Lian-Hui
LH
D001786:Blood Glucose / Q000032:analysis; D002104:Cadmium; D003920:Diabetes Mellitus / Q000175:diagnosis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006029:Glycosuria / Q000175:diagnosis; D006801:Humans; D006861:Hydrogen Peroxide; D008163:Luminescent Measurements / Q000379:methods / Q000592:standards; D019095:Point-of-Care Systems; D045663:Quantum Dots; D013691:Tellurium
D001786:Blood Glucose; D002104:Cadmium; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D013691:Tellurium