Comparison of phenotypic and genotypic antimicrobial profiles in Escherichia coli and Salmonella enterica from the same dairy cattle farms.
Transmission of antimicrobial drug resistance from resistant bacteria to non-resistant strains is an important public health issue. In this study, we have examined the possibility of multiple resistance gene transfer between Escherichia coli and Salmonella in the natural setting. Bacteria isolated from calves concurrently shedding E. coli and Salmonella showed similar antimicrobial drug resistance patterns as measured by a broth dilution method. However, microarray analysis of the antibiotic resistance at the gene level revealed several differences in resistance gene profile. Resistance profiles of E. coli isolated from different farms were closer than the profile of E. coli and Salmonella isolated from the same farm. This shows that the chance of multiple resistance gene transfers between these species is unlikely.
Scaria
Joy
J
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Warnick
Lorin D
LD
Kaneene
John B
JB
May
Katherine
K
Teng
Ching-Hao
CH
Chang
Yung-Fu
YF
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002417:Cattle / Q000382:microbiology*; D016000:Cluster Analysis; D003612:Dairying; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000187:drug effects / Q000235:genetics
D000890:Anti-Infective Agents