Identification of a tumour associated antigen in lung cancer patients with asbestos exposure.
BACKGROUND
This study analysed the humoral immune response in asbestos exposed lung cancer patients to identify new surrogate markers of the carcinogenic risk in populations exposed to asbestos.


RESULTS
A serological analysis identified five distinct antigens reactive with IgG derived from a lung cancer patient with high asbestos exposure. In one of the isolated antigens, quantitative RT-PCR indicated that annexin A2 (AnxA2) was overexpressed in lung cancer tissues and normal lung from patients with high asbestos exposure. Antibody against AnxA2 was detected in 9/15 (60%) of lung cancer patients with high asbestos exposure; however, in only 1/12 (8%) of lung cancer patients with low asbestos exposure. AnxA2 was also overexpressed in malignant mesothelioma cells, and the antibody was also positive in 8/15 (53%) of patients with malignant mesothelioma.


CONCLUSIONS
The antibody titer against AnxA2 may be a potentially useful new diagnostic surrogate marker for asbestos-related lung cancer and malignant mesothelioma.
Yasuda
Manabu
M
Second Department of Surgery, University of Occupational and Environmental Health, Japan, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555 Japan. m-yasuda@med.uoeh-u.ac.jp
Hanagiri
Takeshi
T
Shigematsu
Yoshiki
Y
Onitsuka
Takamitsu
T
Kuroda
Kouji
K
Baba
Tetsurou
T
Mizukami
Makiko
M
Ichiki
Yoshinobu
Y
Uramoto
Hidetaka
H
Takenoyama
Mitsuhiro
M
Yasumoto
Kosei
K
D000368:Aged; D000369:Aged, 80 and over; D017306:Annexin A2 / Q000096:biosynthesis / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001194:Asbestos / Q000276:immunology / Q000506:poisoning*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D008175:Lung Neoplasms / Q000209:etiology* / Q000276:immunology*; D008297:Male; D008654:Mesothelioma / Q000209:etiology / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
C472822:ANXA2 protein, human; D017306:Annexin A2; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007074:Immunoglobulin G; D015850:Interleukin-6; D001194:Asbestos