Germline modification using mouse spermatogonial stem cells.
Spermatogonial stem cells (SSCs) in the testes are a new target for germline modification. With the development of an in vitro culture system and spermatogonial transplantation technique, SSCs can now be manipulated and used as an alternative to embryonic stem cells for knockout mice production. The genetic and epigenetic stability of SSCs provide new possibilities for the application of germline mutagenesis in a wide range of animals.
Kanatsu-Shinohara
Mito
M
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Shinohara
Takashi
T
D000818:Animals; D018929:Cell Culture Techniques; D018095:Germ-Line Mutation / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*