Metabolomic analysis of normal and sickle cell erythrocytes.
Metabolic signatures of specialized circulating hematopoietic cells in physiological or human hematological diseases start to be described. We use a simple and highly reproductive extraction method of erythrocytes metabolites coupled with a liquid chromatography-mass spectrometry based metabolites profiling method to determine metabolomes of normal and sickle cell erythrocytes. Sickle cell erythrocytes and normal erythrocytes metabolomes display major differences in glycolysis, in glutathione, in ascorbate metabolisms and in metabolites associated to membranes turnover. In addition, the amounts of metabolites derived from urea cycle and NO metabolism that partly take place within erythrocyte were different between normal and sickle cell erythrocytes. These results show that metabolic profiling of red blood cell diseases can now be determined and might indicate new biomarkers that can be used for the follow-up of sickle cell patients.
Darghouth
D
D
Laboratoire de recherche sur la réparation et la transcription dans les cellules souches (LRTS), institut de radiobiologie cellulaire et moléculaire (IRCM), direction des sciences du vivant (DSV), commissariat à l'énergie atomique (CEA), BP 6, 18, route du Panorama, 92265 Fontenay-aux-Roses cedex, France.
Koehl
B
B
Junot
C
C
Roméo
P-H
PH
D000755:Anemia, Sickle Cell / Q000097:blood* / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D006019:Glycolysis; D006801:Humans; D055432:Metabolomics; D012016:Reference Values
D005978:Glutathione; D001205:Ascorbic Acid