(-)-Epigallocatechin-3-gallate (EGCG) post-transcriptionally and post-translationally suppresses the cell proliferative protein TROP2 in human colorectal cancer cells.
BACKGROUND
TROP-2 is a tumor-promoting molecule that has been found to be overexpressed in many cancer cells, making it a plausible biomarker of carcinogenesis. The main aim of this study was to examine the effect of green tea catechins (namely, (-)-epigallocatechin-3-gallate; EGCG) on TROP-2 expression.


METHODS
Western blot and RT-PCR were applied to assess TROP2 expression in colorectal cancer cells and tissues.


RESULTS
Two different mechanisms were found to operate in diverse cell lines. In SW480 cells, EGCG affected the post-transcriptional processing of the TROP-2 mRNA, as this was quickly and specifically degraded in the presence of EGCG. In HCT-116 cells, EGCG affected TROP-2 expression at the post-translational level. TROP-2 was found to be highly expressed in colorectal tumors compared to adjacent normal tissues.


CONCLUSIONS
This study provided a novel beneficial activity of green tea as an anti-tumorigenagent causing the suppression of TROP-2 in colorectal cancer.
Sukhthankar
Mugdha
M
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
Alberti
Saverio
S
Baek
Seung Joon
SJ
D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D014176:Protein Biosynthesis / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D016588:Anticarcinogenic Agents; D000951:Antigens, Neoplasm; D000975:Antioxidants; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger; C499380:TACSTD2 protein, human; D002392:Catechin; C045651:epigallocatechin gallate