title: Overexpression, crystallization and preliminary X-ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve.
abstract: The xylulose-5-phosphate/fructose-6-phosphate phosphoketolase gene from Bifidobacterium breve was cloned and overexpressed in Escherichia coli. The enzyme was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. Crystals were obtained at 293 K using 0.05 mM thiamine diphosphate, 0.25 mM MgCl2, 24%(w/v) PEG 6000 and 0.1 M Bicine pH 9.0. The crystals belonged to the tetragonal space group I422, with unit-cell parameters a=b=174.8, c=163.8 A, and diffracted to beyond 1.7 A resolution.
authors.affiliation: Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Suzuki
authors.forename: Byung-Jun
authors.initials: BJ
authors.lastname: Kim
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shibata
authors.forename: Yuki
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: Takane
authors.initials: T
authors.lastname: Katayama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ashida
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Wakagi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shoun
authors.forename: Shinya
authors.initials: S
authors.lastname: Fushinobu
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics; D001644:Bifidobacterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression
chemical_list: D000446:Aldehyde-Lyases; C065556:fructose-6-phosphate phosphoketolase