title: Paraoxonase activity in athletes with depleted iron stores and iron-deficient erythropoiesis.
abstract: OBJECTIVE
  The aim of this study was to evaluate how conditions that precede anaemia (iron store depletion and iron-deficient erythropoiesis) affect human serum paraoxonase PON1 activity.
  
  
  METHODS
  Based on haemoglobin, transferrin saturation and serum ferritin values 119 athletes were divided into three groups: with iron depletion, with deficient erythropoiesis and controls. The following parameters were measured: paraoxonase activity towards paraoxon (POase) and diazoxon (DZOase), lipid hydroperoxides (LOOH), the pro-oxidant-antioxidant balance (PAB), red blood cells (RBC) and lipid status.
  
  
  RESULTS
  Significant differences were found between athletes with different stages of iron deficiency and controls with respect to PON 1 activity and oxidative stress status parameters (Wilks' Lambda=0.712, F=5.241, p<0.001, η(2)=0.156). There was no significant difference between the PON1 192 Q and R polymorphism distribution in the two groups of athletes with different stages of iron deficiency and controls (χ(2)=1.086; p=0.896). PON1 activity was positively correlated with RBCs, haemoglobin, transferrin saturation (p<0.001) and ferritin (p=0.037) and negatively correlated with LOOH (p=0.044) in all three study groups.
  
  
  CONCLUSIONS
  Deficient erythropoiesis in athletes contributes to impaired PON1 activity. In contrast, iron depletion, regardless of increased oxidative stress, does not affect PON1 activity.
authors.affiliation: Belgrade Clinical Centre, Laboratory Department, Belgrade, Serbia. jelena.martinovic@bgdkbc.com
authors.forename: Jelena
authors.initials: J
authors.lastname: Martinović
authors.forename: Jelena
authors.initials: J
authors.lastname: Kotur-Stevuljević
authors.forename: Violeta
authors.initials: V
authors.lastname: Dopsaj
authors.forename: Milivoj
authors.initials: M
authors.lastname: Dopsaj
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Stefanović
authors.forename: Goran
authors.initials: G
authors.lastname: Kasum
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D056352:Athletes; D016022:Case-Control Studies; D004920:Erythropoiesis; D005260:Female; D006439:Hemodynamics; D006801:Humans; D000090463:Iron Deficiencies; D008297:Male; D018384:Oxidative Stress; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D055815:Young Adult
chemical_list: D043303:Aryldialkylphosphatase; C468406:PON1 protein, human