PCR-DGGE-based study of fecal microbial stability during the long-term chitosan supplementation of humans.
A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria.
Mrázek
J
J
Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic, 142 00, Prague 4, Czech Republic.
Koppová
I
I
Kopecný
J
J
Simůnek
J
J
Fliegerová
K
K
D000328:Adult; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification*; D001644:Bifidobacterium / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D048271:Chitosan / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D005260:Female; D006805:Human Experimentation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D048271:Chitosan