[The effect of AGS3 on the I(A) of newborn rat prefrontal cortical neurons pretreated by chronic morphine].
OBJECTIVE
The effect of chronic morphine treatment on the I(A) (transient outward K+ current) of prefrontal cortical neurons of newborn rat. On this basis, we use AGS3 antibody to inhibit the function of AGS3, for observing the impact of AGS3 on the I(A), thus further explore the mechanism of AGS3 protein in morphine addiction.


METHODS
By using whole-cell patch-clamp technique, I(A) was recorded. In the whole-cell configuration, observed the impact of morphine on the current density-voltage curve (I-V) of I(A) and the effect of AGS3 antibody with three different concentrations on the I(A) of morphine treated rat prefrontal cortical neurons.


RESULTS
Morphine increased the I(A). When the test potential was + 55 mV, different concentrations of AGS3, 10(-3) microg/L, 10(-2) micdrog/L and 10(-1) microg/L acted on morphine treated rat prefrontal cortical neurons, the enhanced IA by morphine was inhibited.


CONCLUSIONS
Morphine increases the I(A), AGS3 protein may participate in signal transduction pathway involved with I(A).
Zhao
Ying
Y
Department of Physiology, Luzhou Medical College, Luzhou 646000, China.
Wu
Li-sha
LS
Yang
Ye
Y
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D005260:Female; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects*; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019966:Substance-Related Disorders / Q000378:metabolism / Q000503:physiopathology
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; C498913:Gpsm1 protein, rat; D015221:Potassium Channels; D009020:Morphine