Protective immunization of calves against Ostertagia ostertagi using fourth stage larval extracts.
ConA lectin was used to isolate glycoproteins from detergent extracts of fourth stage Ostertagia ostertagi larvae. This preparation contained proteins additional to those observed in a similar fraction prepared from adult O. ostertagi. Two vaccine trials were conducted with this preparation, and sub-fractions thereof, in groups of 6-8 worm-free calves. All groups were challenged with 50,000 O. ostertagi larvae 1 week after the final immunization, and protection was assessed by comparing the egg and worm counts of the immunized groups with their respective controls. Immunization with the ConA-binding antigen or its sub-fractions induced high titre serum antibody responses. In the first trial, the cumulative egg count of the group immunized with unfractionated antigen was 60% lower than the corresponding control value, and worm counts were 47% lower. In the second trial, the cumulative egg counts of the vaccinated groups ranged from 70% to 85% lower than the corresponding controls, with worm counts up to 64% lower. It was concluded that detergent-soluble, ConA-binding extracts prepared from O. ostertagi fourth stage larvae contained protective immunogens that were as effective as the best antigens published for O. ostertagi to date.
Halliday
A M
AM
Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Scotland, UK. Aileen.Halliday@moredun.ac.uk
Smith
W D
WD
D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000517:prevention & control*; D003208:Concanavalin A / Q000737:chemistry / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D007814:Larva / Q000737:chemistry / Q000276:immunology; D010028:Ostertagia / Q000737:chemistry / Q000276:immunology*; D010029:Ostertagiasis / Q000097:blood / Q000517:prevention & control* / Q000662:veterinary*; D016896:Treatment Outcome; D014612:Vaccines / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D006023:Glycoproteins; D015801:Helminth Proteins; D014612:Vaccines; D003208:Concanavalin A