title: Determination of the G and P types of previously nontypeable rotavirus strains from the African Rotavirus Network, 1996-2004: Identification of unusual G types.
abstract: A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA. mdi4@cdc.gov
authors.forename: Mathew Dioh
authors.initials: MD
authors.lastname: Esona
authors.forename: Duncan
authors.initials: D
authors.lastname: Steele
authors.forename: Tara
authors.initials: T
authors.lastname: Kerin
authors.forename: George
authors.initials: G
authors.lastname: Armah
authors.forename: Ina
authors.initials: I
authors.lastname: Peenze
authors.forename: Annelise
authors.initials: A
authors.lastname: Geyer
authors.forename: Nicola
authors.initials: N
authors.lastname: Page
authors.forename: James
authors.initials: J
authors.lastname: Nyangao
authors.forename: Veronique Akran
authors.initials: VA
authors.lastname: Agbaya
authors.forename: Abdelhalim
authors.initials: A
authors.lastname: Trabelsi
authors.forename: Bizuneh
authors.initials: B
authors.lastname: Tsion
authors.forename: Maryam
authors.initials: M
authors.lastname: Aminu
authors.forename: Theresia
authors.initials: T
authors.lastname: Sebunya
authors.forename: John
authors.initials: J
authors.lastname: Dewar
authors.forename: Roger
authors.initials: R
authors.lastname: Glass
authors.forename: Jon
authors.initials: J
authors.lastname: Gentsch
mesh_terms: D000349:Africa / Q000453:epidemiology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins