[Change of serum soluble CD14 level in newly diagnosed type 2 diabetes and its significance].
OBJECTIVE
To determine the correlation of serum soluble CD14 (sCD14) level with the injury of vascular endothelial cells and chronic low grade inflammation in newly diagnosed Type 2 diabetes (T2DM).


METHODS
ELISA was used to examine serum sCD14 and serum soluble E-selectin (sE-selectin) level, while immunoturbidimetric assay was used to detect serum high sensitivity C reactive protein (hsCRP).


RESULTS
The levels of serum sCD14, sE-selectin, and hsCRP in newly diagnosed T2DM group were higher than those in the euglycemic group [sCD14: (300.7+/-136.6) ng/mL vs. (273.3+/-86.0) ng/mL); sE-selectin: (21.3+/-7.7) ng/mL vs. (32.9+/-11.4) ng/mL; hsCRP: (1.45+/-1.21) mg/L vs. (2.37+/-1.45)mg/L], and there was a significant difference in the latter two parameters between the 2 groups(P<0.01). In the patients with newly diagnosed T2DM, after matching blood pressure, blood sugar, and blood lipid, the levels of serum sCD14, sE-selectin, and hsCRP in the obese group were higher than those in the non-obese group. There was no significant difference in the former 2 parameters between the 2 groups. The serum sE-selectin was correlated with fasting blood sugar (r=0.369, P<0.001), 2-hour postprandial blood sugar (r=0.421, P<0.001), glycosylated hemoglobin (r=0.291, P=0.005), sCD14(r=0.312, P=0.002), and homeostasis model assessment-insulin resistance(r=0.247, P=0.018) in the newly diagnosed T2DM group. Stepwise regression ana-lysis showed that the serum sCD14 was one of the chief influencing factors on serum sE-selectin.


CONCLUSIONS
Serum sCD14 levels tend to increase in newly diagnosed T2DM patients, especially in the obese diabetic patients, which is one of the chief influencing factors to induce the injury of vascular endothelial cells. The innate immunity mediated by Toll-like receptor 4 may take part in the injury of vascular endothelial cells in newly diagnosed T2DM patients.
Mo
Hui
H
Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Liu
Shiping
S
Zhou
Zhiguang
Z
Tang
Weili
W
Yan
Xiang
X
Huang
Gan
G
Li
Jianwei
J
Feng
Qiong
Q
D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000276:immunology; D019040:E-Selectin / Q000097:blood; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D012995:Solubility; D051197:Toll-Like Receptor 4 / Q000276:immunology
D019040:E-Selectin; D018950:Lipopolysaccharide Receptors; C546936:SELE protein, human; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D002097:C-Reactive Protein