Salt concentration-induced dehybridisation of DNA-gold nanoparticle conjugate assemblies for diagnostic applications.
We present the cooperative dehybridisation of DNA-gold nanoparticle conjugate (DNA-AuNP) assemblies induced by reduced salt concentration ([salt]), which can be precisely controlled by various conditions. The detection of Ag(+) based upon the [salt]-induced dehybridisation of DNA-AuNP assemblies is five times more sensitive than that achieved under conventional thermal melting conditions.
Oh
Ju-Hwan
JH
Department of Materials Science and Engineering and Institute for Biomedical Research, Korea University, Anam-dong, Seongbuk-gu, Seoul, Republic of Korea 136-713.
Lee
Jae-Seung
JS
D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000032:analysis*; D012965:Sodium Chloride / Q000737:chemistry*
D012834:Silver; D012965:Sodium Chloride; D006046:Gold; D004247:DNA