Cryopreservation of mouse gametes and embryos.
As the use of genetically engineered mouse models continues to expand, the need to cryopreserve strains of mice increases in parallel in order to preserve these unique research resources and provide a low-cost alternative to maintaining the large inventory of strains. This chapter discusses methods for the cryopreservation of mouse embryos, sperm, and oocytes, and briefly discusses other requirements for implementing a successful cryopreservation program.
Landel
Carlisle P
CP
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation / Q000379:methods; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology; D011786:Quality Control; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology
D003470:Culture Media