[Research on immune system response, skin and hepatic reactivity in laboratory animals after retro-auricular topical administration of two reactive textile dyes].
OBJECTIVE
To evaluate immune system response, skin and hepatic reactivity in Swiss mice after retro auricular local administration of two textile dyes.


METHODS
On 3 groups of white Swiss mice: group I (Cibacron Yellow F-4G), group II (Cibacron Orange P-2R) and control group (DMSO dimethylsulfoxide), substances were applied on left retro auricular ear, in dose of 25 microL solution 10% in DMSO (the dyes were not soluble in water), once a day, for 7 days. After euthanasia, blood samples were taken to assay differential cell count, peripheral neutrophils activity (NBT test), serum opsonic capacity, peritoneal macrophages activity (phagocytic and bactericidal capacity), and activity of spleenic T-lymphocytes with rossetting capacity, and spleen cells forming Jerne plaques. There were taken skin from local application of dyes, retro auricular and later cervical nodes from left side and hepatic samples.


RESULTS
Retro auricular and later cervical nodes mass increases after administration of textile dyes, compared to control group. Basophiles percentages were increased after administration of two dyes (stronger after yellow dye). Peripheral neutrophils activity was increased after administration of two dyes, especially after orange dye. Administration of the two dyes decreased serum opsonic capacity and peritoneal macrophages activity, but did not influenced the activity of spleenic T-lymphocytes with rossetting capacity and spleen cells forming Jerne plaques. There were significant skin and hepatic changes in microscopy.


CONCLUSIONS
The studied dyes influenced the activity of immune system, increased skin and hepatic reactivity.
Gavăt
C
C
Universitatea de Medicină şi Farmacie Gr.T. Popa Iaşi Facultatea de Medicină, Disciplina de Chimie, Facultatea de Medicină.
Ghiciuc
Cristina Mihaela
CM
Zamfir
Carmen Lăcrămioara
CL
Lupuşoru
R V
RV
Lupuşoru
Cătălina Elena
CE
D000279:Administration, Cutaneous; D000818:Animals; D001391:Azo Compounds / Q000633:toxicity; D004396:Coloring Agents / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004431:Ear, External; D007155:Immunologic Factors / Q000276:immunology; D008099:Liver / Q000187:drug effects* / Q000276:immunology*; D023441:Local Lymph Node Assay; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology; D012867:Skin / Q000187:drug effects* / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013783:Textile Industry; D014227:Triazines / Q000633:toxicity
D001391:Azo Compounds; D004396:Coloring Agents; D007155:Immunologic Factors; D009895:Opsonin Proteins; D014227:Triazines