Preparation and analysis of microRNA libraries using the Illumina massively parallel sequencing technology.
MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.
Morin
Ryan D
RD
BC Cancer Agency, Genome Sciences Centre, Vancouver, BC, Canada.
Zhao
Yongjun
Y
Prabhu
Anna-Liisa
AL
Dhalla
Noreen
N
McDonald
Helen
H
Pandoh
Pawan
P
Tam
Angela
A
Zeng
Thomas
T
Hirst
Martin
M
Marra
Marco
M
D053595:Embryonic Stem Cells / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
D035683:MicroRNAs