title: A study of bone marrow neoangiogenesis in chronic lymphocytic leukemia patients.
abstract: OBJECTIVE
  To use a purely geometric data-unravelling method to identify, without a priori, direct links of neoangiogenesis with known clinicobiologic variables in 23 untreated chronic lymphocytic leukemia (CLL) patients at the time of marrow sampling.
  
  
  METHODS
  We measured neovascular surface density (CD34 endothelial marker) in the 10 most labeled fields (hVA), cellularity (CA) and the percentage of avascular fields (AVF) in bone marrow biopsy specimens from 34 patients with CLL. Vascular endothelial growth factor (VEGF) was assayed in thawed serum samples from 8 of the 23 untreated patients (12 Rai stage 0, 6 Rai stage I-II, 5 Binet stage B-C).
  
  
  RESULTS
  Neoangiogenesis hVA was lower in the 12 stage O patients (p < 0.039) and in the 6 patients with nodular interstitial mixed infiltration (p = 0.058). The link between hVA and serum VEGF (sVEGF) was negative in 8 patients (R = -0.49, -0.70 for a given age). Age was linked to moderate interstitial infiltration (p < 0.02), AVF (R = 0.37) and lower sVEGF levels (R = -0.676). The blood platelet count showed numerous correlations, including links with AVF (R = 0.40), low hVA (R = -0.38), female sex (p = 0.068), absence of splenomegaly (p < 0.001), mixed-type infiltration (p < 0.01) and sVEGF (R = 0.38, 0.61 for a given age).
  
  
  CONCLUSIONS
  The links revealed by the iconography of correlations were described statistically. Surprisingly, negative links of hVA with the sVEGF level, 2 known prognostic factors, on the one hand, and with the blood platelet count, on the other hand, were found. The direct link between disease progression and the amount of avascular hematopoietic tissue (NS), not previously described, will require further study. Age should be taken into account.
authors.affiliation: Laboratory of Biologic Hematology, Hôpital de la Pitié-Salpêtrière, Paris, France. claude.lesty@upmc.fr
authors.forename: Claude
authors.initials: C
authors.lastname: Lesty
authors.forename: Sylvie
authors.initials: S
authors.lastname: Baudet
authors.forename: Frederic
authors.initials: F
authors.lastname: Charlotte
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001706:Biopsy; D001808:Blood Vessels / Q000378:metabolism / Q000473:pathology; D001853:Bone Marrow / Q000098:blood supply*; D018450:Disease Progression; D005260:Female; D006403:Hematologic Tests; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000209:etiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A