title: Mass spectrometry-based metabolomics, analysis of metabolite-protein interactions, and imaging.
abstract: Our understanding of biology has been greatly improved through recent developments in mass spectrometry, which is providing detailed information on protein and metabolite composition as well as protein-metabolite interactions. The high sensitivity and resolution of mass spectrometry achieved with liquid or gas chromatography allows for detection and quantification of hundreds to thousands of molecules in a single measurement. Where homogenization-based sample preparation and extraction methods result in a loss of spatial information, mass spectrometry imaging technologies provide the in situ distribution profiles of metabolites and proteins within tissues. Mass spectrometry-based analysis of metabolite abundance, protein-metabolite interactions, and spatial distribution of compounds facilitates the high-throughput screening of biochemical reactions, the reconstruction of metabolic networks, biomarker discovery, determination of tissue compositions, and functional annotation of both proteins and metabolites.
authors.affiliation: Department of GTL Bioenergy and Structural Biology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Do Yup
authors.initials: DY
authors.lastname: Lee
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Bowen
authors.forename: Trent R
authors.initials: TR
authors.lastname: Northen
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D006801:Humans; D021621:Imaging, Three-Dimensional; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins