title: Cryopreservation of mouse gametes and embryos.
abstract: As the use of genetically engineered mouse models continues to expand, the need to cryopreserve strains of mice increases in parallel in order to preserve these unique research resources and provide a low-cost alternative to maintaining the large inventory of strains. This chapter discusses methods for the cryopreservation of mouse embryos, sperm, and oocytes, and briefly discusses other requirements for implementing a successful cryopreservation program.
authors.affiliation: Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
authors.forename: Carlisle P
authors.initials: CP
authors.lastname: Landel
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D004624:Embryo Transfer / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation / Q000379:methods; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology; D011786:Quality Control; D012662:Semen Preservation / Q000295:instrumentation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology
chemical_list: D003470:Culture Media