title: The effect of a single bolus of exogenous surfactant on lung compliance persists until two weeks after treatment in a model of acid aspiration pneumonitis.
abstract: Aspiration pneumonitis, a direct cause of acute lung injury, is associated to a severe deterioration of lung function due in part to endogenous surfactant system impairment. We evaluated the effects of selective exogenous surfactant administration in a murine model of acid-induced lung injury both at short and long term. Hydrochloric acid was instilled into the right bronchus of the mice (0.1 M, 1.5 ml/kg). Six hours from acid instillation, they received, in the injured lung, a 1 ml/kg bolus of porcine surfactant (Curosurf®) 40 or 80 mg phospholipids/ml (Surf₄₀ or Surf₈₀), or saline (0.9% NaCl, vehicle) Functional, biochemical, histological and inflammatory results were obtained 24 h after injury. In the Surf₈₀ and vehicle group, lung mechanics and histology were assessed again after two weeks. Micro-Computed Tomography scans were performed at 24 h and two weeks. 24 h after injury in the Surf₈₀ group respiratory system compliance and oxygenation were significantly improved compared to the vehicle, while neutrophilic fraction in bronchoalveolar lavage was significantly lower. Surfactant-treated mice maintained a better compliance and a significantly reduced inflammatory infiltrate also two weeks after treatment compared to vehicle. Micro-Computed Tomography scan suggested a more pronounced reduction of injury in surfactant-treated animals two weeks after injury (p = 0.09). We showed a beneficial effect on lung function of a single bolus of exogenous surfactant in unilateral acid-induced lung injury up to two weeks after treatment. Our results may suggest a role of surfactant replacement in modulating spontaneous evolution of injury.
authors.affiliation: Department of Experimental Medicine, University of Milano-Bicocca, Monza, Italy; Department of Perioperative Medicine and Intensive Care, San Gerardo Hospital, Monza, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Scanziani
authors.forename: Maria
authors.initials: M
authors.lastname: Amigoni
authors.forename: Giacomo
authors.initials: G
authors.lastname: Bellani
authors.forename: Vanessa
authors.initials: V
authors.lastname: Zambelli
authors.forename: Serge
authors.initials: S
authors.lastname: Masson
authors.forename: Enrico
authors.initials: E
authors.lastname: Radaelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Pesenti
authors.forename: Roberto
authors.initials: R
authors.lastname: Latini
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D008170:Lung Compliance / Q000187:drug effects*; D051379:Mice; D011015:Pneumonia, Aspiration / Q000188:drug therapy* / Q000401:mortality / Q000503:physiopathology; D011663:Pulmonary Surfactants / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D011663:Pulmonary Surfactants