Sequence-specific primers for MNS blood group genotyping.
BACKGROUND
Various techniques of genotyping the MNSs blood group have been described, but none of them enables the complete detection of all MNS antigens.


METHODS
Blood samples were obtained from blood donors. Primers were created using the published DNA sequences for glycophorins A and B. Genotyping was performed using polymerase chain reaction sequence-specific primers (PCR-SSP).


RESULTS
A total of seven primers were found to specifically amplify the most common MNS antigens. The use of these primers has enabled us to correctly genotype all blood samples tested so far (n=116).


CONCLUSIONS
Specifically created primers enable genotyping of the MNS antigens in a single PCR-SSP run. The method is reliable, easy to perform, and can be used in routine practice.
Heymann
Guido A
GA
Central Department of Laboratory Medicine, DRK-Kliniken-Berlin, USA. g.heymann@drk-kliniken-berlin.de
Salama
Abdulgabar
A
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D017931:DNA Primers; D005838:Genotype; D006021:Glycophorins / Q000235:genetics; D006801:Humans; D008951:MNSs Blood-Group System / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D006021:Glycophorins; D008951:MNSs Blood-Group System