Succinate cytochrome c reductase in schistosomiasis: in vitro inhibition by some schistosomicidal drugs.
Enzymes in mitochondria play an important role in biological oxidation and energy production. To understand the effect of schistosomiasis on these important processes, succinate cytochrome c reductase (SCR) from control and Schistosoma-infected mice was subjected for investigation. In this article, we report that SCR from Schistosoma-infected mouse showed a significant decrease in its Vmax and Km compared to control using both cytochrome c and 2,6-dichlorophenolindophenol as substrates. Furthermore, the kinetic studies of the purified SCR in the absence and presence of the schistosomicidal drugs praziquantel and Commiphora extract reveal that both drugs have an inhibitory action on the enzyme from the control and Schistosoma-infected mice and praziquantel changes the type of inhibition of SCR towards cytochrome c from mixed type in control to a competitive one in the case of the infection.
Balbaa
Mahmoud
M
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt. m_balbaa@hotmail.com
Abdel Moneam
Nihad M
NM
El-Kersh
Mohamed
M
Omran
Heba
H
Kandeel
Kamal
K
D015086:2,6-Dichloroindophenol / Q000737:chemistry; D000818:Animals; D006358:Hot Temperature; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology; D011223:Praziquantel / Q000494:pharmacology; D015203:Reproducibility of Results; D012552:Schistosomiasis / Q000201:enzymology*; D012556:Schistosomicides / Q000627:therapeutic use*; D013384:Succinate Cytochrome c Oxidoreductase / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D013696:Temperature
D012556:Schistosomicides; D011223:Praziquantel; D015086:2,6-Dichloroindophenol; D013384:Succinate Cytochrome c Oxidoreductase