title: β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae.
abstract: A peptidogalactomannan was isolated from mycelia of Cladosporium (Hormoconis) resinae and characterized using methylation-fragmentation analysis, partial acid hydrolysis and ¹H and ¹³C-NMR spectroscopy. The galactomannan component was a branched structure and consisted of a main chain containing (1→6)-linked α-d-Manp residues substituted at O-2 by side chains containing (1→2)-linked α-D-Manp residues. β-D-Galf residues were present as side chains of 3-4 units that are (1→5)-interlinked. This structure is very similar to a pGM isolated from Aspergillus fumigatus and differs from that of Cladosporium werneckii (currently named Hortaea werneckii), with this pGM and other fungal galactomannans having single terminal (1→6)-linked β-Galf residues. The importance of the carbohydrate moiety of Cladosporium resinae pGM in immunoassays was also demonstrated. On FACS examination, a decrease (60%) in rabbit serum anti- C. resinae binding to C. resinae conidia occurred when this serum had been previously incubated with pGMs from C. resinae and A. fumigatus or with mannoprotein from Candida parapsilosis, suggesting the presence of cross-reactive determinants in these fungi.
authors.affiliation: Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (UFRJ),Bloco I, Ilha do Fundão, 21941-970 Rio de Janeiro, RJ, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Calixto
authors.forename: Bianca
authors.initials: B
authors.lastname: Mattos
authors.forename: Vera
authors.initials: V
authors.lastname: Bittencourt
authors.forename: Lívia
authors.initials: L
authors.lastname: Lopes
authors.forename: Lauro
authors.initials: L
authors.lastname: Souza
authors.forename: Guilherme
authors.initials: G
authors.lastname: Sassaki
authors.forename: Thales
authors.initials: T
authors.lastname: Cipriani
authors.forename: Maria
authors.initials: M
authors.lastname: Silva
authors.forename: Eliana
authors.initials: E
authors.lastname: Barreto-Bergter
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000737:chemistry / Q000276:immunology; D002473:Cell Wall / Q000737:chemistry*; D002963:Cladosporium / Q000737:chemistry / Q000648:ultrastructure*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D005658:Fungi; D005690:Galactose / Q000031:analogs & derivatives* / Q000032:analysis*; D006020:Glycopeptides / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis / Q000737:chemistry* / Q000276:immunology; D008745:Methylation; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D011817:Rabbits
chemical_list: D000946:Antigens, Fungal; D005656:Fungal Proteins; D006020:Glycopeptides; D008351:Mannans; C012990:galactomannan; D005690:Galactose