An automated and highly efficient method for counting and measuring fluorescent foci in rod-shaped bacteria.
Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually, and without clear definition of how they are made. Here we present a procedure for standardizing the measurement of cell properties from phase contrast images. Furthermore, we present a program using these standardized methods that can measure the intracellular positions of fluorescent foci in bacterial cells faster and with more precision than manual measurement.
Nielsen
H J
HJ
Department of Systems Biology, Building 301, Technical University of Denmark, Lyngby, Denmark.
Hansen
F G
FG
D048168:Escherichia coli K12 / Q000166:cytology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*