title: MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells.
abstract: MicroRNAs (miRNAs) are endogenous non-coding small RNAs that inhibit gene expression post-transcriptionally. By regulating their target genes, miRNAs play important roles in tumor generation and development. Recently, the mir-200 family was revealed to inhibit the epithelial-mesenchymal transition, which is viewed as an essential step in early tumor metastasis. Here, we used luciferase assays to demonstrate that mir-200b interacts with predicted target sites in the 3' untranslated region of RND3. In HeLa cells, mir-200b directly reduced the expression of RND3 at the mRNA and protein levels, which thereby promoted expression of the downstream protein cyclin D1 and increased S-phase entry. In conclusion, our study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Liucun
authors.initials: L
authors.lastname: Chen
authors.forename: Baochun
authors.initials: B
authors.lastname: Huang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Nongle
authors.initials: N
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Fang
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Tianyou
authors.initials: T
authors.lastname: Wang
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D056741:Cyclin D / Q000235:genetics*; D017931:DNA Primers; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D020741:rho GTP-Binding Proteins / Q000502:physiology*
chemical_list: D056741:Cyclin D; D017931:DNA Primers; D035683:MicroRNAs; C508705:RND3 protein, human; D020741:rho GTP-Binding Proteins