title: Polysaccharides from Capsosiphon fulvescens stimulate the growth of IEC-6 Cells by activating the MAPK signaling pathway.
abstract: Seaweed extracts show diverse bioactivities, such as antioxidant and antitumor activity. Capsosiphon fulvescens is a green alga that is abundant along the southwest coast of South Korea. Although it is consumed for its purported health-enhancing properties, particularly as a treatment for stomach disorders and hangovers, the health effects of dietary C. fulvescens remain unclear. We extracted polysaccharides from C. fulvescens (Cf-PS), investigated their effects on the proliferation of rat small intestinal epithelial IEC-6 cells, and determined the signaling cascade involved. We cultured IEC-6 cells in the presence of Cf-PS, which stimulated cell proliferation in a dose-dependent manner, and analyzed the Wnt and MAPK signaling pathways, which are related to cell proliferation. Cf-PS treatment induced the translocation of β-catenin, an effector of the Wnt signaling pathway, from the cytosol to the nucleus and increased the expression of cyclinD1 and c-myc. Cf-PS also induced ERK1/2 phosphorylation, which is activated by mitogenic and proliferative stimuli such as growth factors, but the phosphorylation of JNK and p38 was not enhanced. Our results show that Cf-PS regulates proliferation via stimulating the nuclear translocation of β-catenin and ERK1/2 activation in intestinal epithelial cells.
authors.affiliation: Food Science and Biotechnology, Pukyong National University, Busan, South Korea.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Go
authors.forename: Hye-Jung
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Taek-Jeong
authors.initials: TJ
authors.lastname: Nam
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D000460:Chlorophyta / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D008853:Microscopy; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D056910:Republic of Korea; D015398:Signal Transduction / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D051176:beta Catenin; D020928:Mitogen-Activated Protein Kinases