title: Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARalpha.
abstract: The interleukin-10 gene-deficient (Il10(-/-)) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10(-/-) model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARalpha and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10(-/-) mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in 12-week-old Il10(-/-) mice. Gene expression changes in 12-week-old Il10(-/-) mice were related to PPARalpha signaling likely to control colitis, but how PPARalpha activation might regulate intestinal IL10 production remains to be determined.
authors.affiliation: Food, Metabolism & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New Zealand.
authors.forename: Bianca
authors.initials: B
authors.lastname: Knoch
authors.forename: Matthew P G
authors.initials: MP
authors.lastname: Barnett
authors.forename: Janine
authors.initials: J
authors.lastname: Cooney
authors.forename: Warren C
authors.initials: WC
authors.lastname: McNabb
authors.forename: Diane
authors.initials: D
authors.lastname: Barraclough
authors.forename: William
authors.initials: W
authors.lastname: Laing
authors.forename: Shuotun
authors.initials: S
authors.lastname: Zhu
authors.forename: Zaneta A
authors.initials: ZA
authors.lastname: Park
authors.forename: Paul
authors.initials: P
authors.lastname: Maclean
authors.forename: Scott O
authors.initials: SO
authors.lastname: Knowles
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Roy