A transcriptome-based examination of blood group expression.
Over the last two decades, red cell biologists witnessed a vast expansion of genetic-based information pertaining to blood group antigens and their carrier molecules. Genetic progress has led to a better comprehension of the associated antigens. To assist with studies concerning the integrated regulation and function of blood groups, transcript levels for each of the 36 associated genes were studied. Profiles using mRNA from directly sampled reticulocytes and cultured primary erythroblasts are summarized in this report. Transcriptome profiles suggest a highly regulated pattern of blood group gene expression during erythroid differentiation and ontogeny. Approximately one-third of the blood group carrier genes are transcribed in an erythroid-specific fashion. Low-level and indistinct expression was noted for most of the carbohydrate-associated genes. Methods are now being developed to further explore and manipulate expression of the blood group genes at all stages of human erythropoiesis.
Noh
S-J
SJ
Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), 31 Center Drive, Bethesda, Building 10, Room 9N311, MD 20892, USA.
Lee
Y T
YT
Byrnes
C
C
Miller
J L
JL
D000328:Adult; D018952:Antigens, CD34 / Q000502:physiology; D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D004900:Erythroblasts / Q000166:cytology / Q000502:physiology; D004912:Erythrocytes / Q000502:physiology; D004920:Erythropoiesis / Q000235:genetics*; D005312:Fetal Blood / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006021:Glycophorins / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D011990:Receptors, Transferrin / Q000235:genetics; D012156:Reticulocytes / Q000502:physiology; D014158:Transcription, Genetic
D018952:Antigens, CD34; D001789:Blood Group Antigens; D006021:Glycophorins; D012333:RNA, Messenger; D011990:Receptors, Transferrin