FGF-2 and VEGF functionalization of starPEG-heparin hydrogels to modulate biomolecular and physical cues of angiogenesis.
Tissue engineering therapies require biomaterials capable of encouraging an angiogenic response. To dissect the influence of different pro-angiogenic stimuli a set of starPEG-heparin hydrogels with varied physicochemical properties was used as a highly efficient reservoir and tunable delivery system for basic fibroblast growth factor (FGF-2) and vascular endothelial growth factor (VEGF). The engineered gel materials could be precisely tailored by decoupling the biomolecular functionalization from the variation of the viscoelastic matrix characteristics. Culture experiments with human umbilical vein endothelial cells (HUVECs) revealed the interplay of growth factor presentation, adhesive characteristics and elasticity of the gel matrices in triggering differential cellular behavior which allowed identifying effective pro-angiogenic conditions.
Zieris
Andrea
A
Leibniz Institute of Polymer Research Dresden (IPF), Max Bergmann Center of Biomaterials Dresden (MBC) & Technische Universität Dresden (TUD), Dresden, Germany.
Prokoph
Silvana
S
Levental
Kandice R
KR
Welzel
Petra B
PB
Grimmer
Milauscha
M
Freudenberg
Uwe
U
Werner
Carsten
C
D001672:Biocompatible Materials / Q000494:pharmacology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005453:Fluorescence; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism*; D055767:Immobilized Proteins / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism; D054457:Tissue Scaffolds; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D001672:Biocompatible Materials; D020100:Hydrogels; D055767:Immobilized Proteins; D012235:Rhodamines; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D006493:Heparin