title: [Screening, identification and analysis of platelet differential functional proteins in patients with coronary heart disease of blood-stasis pattern].
abstract: OBJECTIVE
  To seek the key platelet functional proteins (PFPs) for the occurrence of blood-stasis pattern (BP) in patients with coronary heart disease (CHD).
  
  
  METHODS
  Peripheral blood platelet protein of 22 patients and 24 healthy person (for control) were extracted respectively in 4 batches for carrying 4 times of the test out. Differential PFPs in samples were screened out by two-dimensional fluorescence difference gel electrophoresis, and identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry; then the identified proteins were further authenticated by Western-blotting.
  
  
  RESULTS
  Thirteen differential PFPs were screened out, and among them the 7 identified by spectrometry were: isoform 1 of integrin alpha- II b, isoform 2 of integrin alpha- II b, actin-cytoplasmic 1, actin-cytoplasmic 2, cDNA FLJ52842, cDNA FLJ55253, and cDNA FLJ43573 fis. Among them isoform 2 of integrin alpha- II b (CD41) and actin-cytoplasmic 2 (Acting) were authenticated successfully.
  
  
  CONCLUSIONS
  CD41 and acting are the possible marker proteins, and the other PFPs might play crucial roles in the occurrence and development of BSS in CHD.
authors.affiliation: Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Yue-Rong
authors.initials: YR
authors.lastname: Jiang
authors.forename: Zhu-Ye
authors.initials: ZY
authors.lastname: Gao
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets; D016022:Case-Control Studies; D003327:Coronary Disease / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010980:Platelet Membrane Glycoproteins