Effect of sesame lignans on TNF-alpha-induced expression of adhesion molecules in endothelial cells.
The expression of cell adhesion molecules (CAMs) has been implicated as one of the most important causes of the development of inflammatory diseases such as atherosclerosis, and, it is speculated that the prevention of it is an effective approach to the control of atherosclerosis. In the present study, we investigated the effect of sesame lignans on the expression of CAMs in human umbilical vein endothelial cells (HUVECs) induced by tumor necrosis factor-alpha (TNF-alpha). Based on cell-ELISA analysis, we found that sesaminol-6-catechol downregulated the TNF-alpha-induced expression of CAMs in a dose-dependent manner. Moreover, these inhibitory effects were caused to be drastically exerted by downregulating the CAM proteins in TNF-alpha-activated HUVECs at transcriptional level. This suggests, that sesaminol-6-catehcol suppresses the expression of CAMs, and may be an active component of sesame lignans.
Mochizuki
Mika
M
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, Iwasaki-cho, Nisshin, Aichi, Japan. mochi@dp.aichi-gakuin.ac.jp
Tsuchie
Yoshikazu
Y
Yamada
Naruomi
N
Miyake
Yoshiaki
Y
Osawa
Toshihiko
T
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002319:Cardiovascular System / Q000187:drug effects / Q000378:metabolism; D002396:Catechols / Q000737:chemistry / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D031685:Sesamum / Q000737:chemistry*; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D002396:Catechols; D015815:Cell Adhesion Molecules; D017705:Lignans; D014409:Tumor Necrosis Factor-alpha; C034221:catechol