[Effect of endogenous H2S on platelet L-Arg transport].
OBJECTIVE
To observe the effect of novel air neuromodulator H2S on platelet function of L-Arg transport for discussing H2S of effect on platelet function.


METHODS
Saturate H2S solution as donate made rat rich platelet plasma and pre-incubation rat platelet with different density of H2S. To measure the velocity of L-Arg transport in platelet by radioactivity technique.


RESULTS
At different concentrations of H2S (6.25, 12.5, 25, 50, 100 micromol/L), the velocity of L-Arg transport was lower than that in control. H2S reduced rapidly the Vmax and velocity of L-Arg transport in platelet (P < 0.05) and this effect had no effect to Km.


CONCLUSIONS
H2S can affect platelet function by changing rapidly platelet L-Arg transport system function.
Duan
Wen-zhuo
WZ
Weifang Medical College, Weifang 261042, China. dwenzhy@hotmail.com
Wang
Yi-peng
YP
Gong
Hai-min
HM
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D001120:Arginine; D006862:Hydrogen Sulfide