Polymorphisms in NAT2 gene and atherosclerosis in an Algerian population.
OBJECTIVE
The etiology of atherosclerosis is multifactorial. Genetic and environmental factors are involved in the development of atherosclerosis. Human arylamine N-acetyltransferase 2 (NAT2) is an important metabolizing enzyme that exhibits genetic polymorphisms and modifies individual response and/or toxicity to many xenobiotics. We undertook this study to investigate the NAT2 polymorphisms in patients with atherosclerosis.


METHODS
Genotyping for NAT2 alleles was performed using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) in 285 Algerian patients with atherosclerosis and 286 controls.


RESULTS
There was no association between NAT2 polymorphisms and atherosclerosis risk. However, the haplotype NAT2(*)5F decreased susceptibility to the disease (p = 0.005, OR = 0.55, 95% CI = 0.37-0.84). The frequency of the slow acetylator phenotype was approximately 50% in both cases and controls.


CONCLUSIONS
These results suggest that NAT2 polymorphisms may not be involved in the pathogenesis of atherosclerosis.
Khelil
Malika
M
Département de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari, Boumediène, Alger, Algérie. malikakhelil@hotmail.fr
Zenati
Akila
A
Makrelouf
Mohamed
M
Otmane
Amel
A
Tayebi
Bouchentouf
B
D000328:Adult; D000462:Algeria; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D050197:Atherosclerosis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors
D017931:DNA Primers; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human