title: A quartz crystal microbalance sensor based on mussel-inspired molecularly imprinted polymer.
abstract: In this work, we describe a simple, inexpensive and fast method for the generation of molecularly imprinted polymer (MIP) film on quartz crystal microbalance (QCM) crystals using mussel-inspired polymer. Commonly known as a neurotransmitter, dopamine is also a small-molecule mimic of the adhesive proteins of mussels. Polymerization of dopamine in the presence of template molecule (1,3,5-pentanetricarboxylic acid, an analogue of domoic acid, in this case) could produce an adherent molecularly imprinted polydopamine film coating on QCM crystals. Advantages, such as high hydrophilicity, high biocompatibility and controllable thickness, make this molecularly imprinted polydopamine film an attractive recognition element for sensors. Selective rebinding of domoic acid on mussel-inspired molecularly imprinted polymer (m-MIP) coated crystal was observed as a frequency shift quantified by piezoelectric microgravimetry with the QCM system. The decreasing frequency shows a good linear relationship with the concentration of domoic acid. The quantitation limit of domoic acid was 5 ppb with the linear range of 0-100 ppb. The QCM sensor has high selectivity and was able to distinguish domoic acid from its analogous p-phthalic acid and o-phthalic acid owing to the molecular imprinting effect. In addition, the practical analytical performance of the sensor was examined by evaluating the detection of domoic acid in mussel extracts with satisfactory results. It is envisaged that m-MIP could be suitable as recognition element for sensors and the proposed m-MIP QCM sensor could be employed to detect analyte of interest in complex matrices.
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, PR China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zhou
authors.forename: Shui-Fen
authors.initials: SF
authors.lastname: Tang
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Yao
authors.forename: Fa-Rong
authors.initials: FR
authors.lastname: Chen
authors.forename: Huang-Hao
authors.initials: HH
authors.lastname: Yang
authors.forename: Xiao-Ru
authors.initials: XR
authors.lastname: Wang
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D015374:Biosensing Techniques / Q000295:instrumentation*; D049872:Bivalvia / Q000033:anatomy & histology* / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011108:Polymers; D004298:Dopamine