Evaluation of liver and thyroid toxicity in Sprague-Dawley rats after exposure to polybrominated diphenyl ether BDE-209.
Our goal in the present study was to evaluate whether decabromodiphenyl ether (BDE-209), which is the most abundant polybrominated diphenyl ether (PBDE) found in human samples, affects against target organs. Sprague-Dawley male rats were exposed to vehicle or BDE-209 (100, 300, or 600 mg/kg body weight, daily) from postnatal day (PND) 10 to PND 42. There was no significant difference in body and male reproductive organ weight changes compared with controls. However, liver, thyroid and adrenal gland weights were significantly increased in the high-dose of BDE-209 group. BDE-209 significantly induced the expression of cytochrome P450 (CYP1A2, CYP3A1, and CYP2B1) enzymes in the liver. Furthermore, constitutive androstane receptor (CAR) and pregnane xenobiotic receptor (PXR) expression levels were also increased in a dose-dependent manner. Total serum triiodothyronine (T3) concentration was significantly reduced in a dose-dependent manner, whereas the level of thyroid-stimulating hormone was significantly increased with BDE-209 treatment. In the histological findings, multiple areas of degenerated follicular epithelium and slight attenuation of the follicular epithelium were observed in the thyroid glands by high doses (300 and 600 mg/kg) of BDE-209 treatment. The presence of hepatocytic fatty degeneration and inflammatory foci were also observed in the 300 and 600 mg/kg of BDE-209 group. These findings demonstrate that BDE-209 induces hyperthyroidism and hepatotoxicity. In the future, further research is needed to determine the relationship between target organ toxicity and blood concentrations of BDE-209.
Lee
Ena
E
College of Pharmacy, Pusan National University, Busan, Korea.
Kim
Tae Hyung
TH
Choi
Jae Seok
JS
Nabanata
Patra
P
Kim
Na Young
NY
Ahn
Mee Young
MY
Jung
Ki Kyung
KK
Kang
Il Hyun
IH
Kim
Tae Sung
TS
Kwack
Seung Jun
SJ
Park
Kui Lea
KL
Kim
Seung Hee
SH
Kang
Tae Seok
TS
Lee
Jaewon
J
Lee
Byung Mu
BM
Kim
Hyung Sik
HS
D000818:Animals; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013961:Thyroid Gland / Q000187:drug effects*
D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether