Overexpression of MMP-13 gene in colorectal cancer with liver metastasis.
Matrix metalloproteinase-7 (MMP-7), MMP-9, MMP-13, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are considered to have important roles in the invasiveness and outcomes of colorectal cancer (CRC). This study examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially as related to liver metastasis. The study analysed surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated colorectal cancer. MMP-7, MMP-9, MMP-13, TIMP-1, and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time, reverse-transcriptase polymerase chain reaction. Expression levels of MMP-7, MMP-9, MMP-13 and TIMP-1 were higher in cancer tissue than in adjacent normal mucosa. On analysis of the relations between gene expression and clinicopathological factors, MMP-13 expression was found to correlate with liver metastasis. Moreover, MMP-13 expression levels were higher in tumour tissue with liver metastasis than in that without liver metastasis. It is concluded that MMP-13 gene expression is a useful predictor of liver metastasis in patients with CRC.
Yamada
Takanobu
T
Department of Gastrointestinal Surgery, Kanagawa Cancer Center, 1-1-2 Nakao, Asahi-ku, Yokohama-shi, Kanagawa-ken 241-0815, Japan. takay0218@yahoo.co.jp
Oshima
Takashi
T
Yoshihara
Kazue
K
Tamura
Shuzo
S
Kanazawa
Amane
A
Inagaki
Daisuke
D
Yamamoto
Naoto
N
Sato
Tsutomu
T
Fujii
Shoichi
S
Numata
Kazushi
K
Kunisaki
Chikara
C
Shiozawa
Manabu
M
Morinaga
Soichiro
S
Akaike
Makoto
M
Rino
Yasushi
Y
Tanaka
Katsuaki
K
Masuda
Munetaka
M
Imada
Toshio
T
D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis* / Q000235:genetics; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics
D019715:Tissue Inhibitor of Metalloproteinase-1; D053509:Matrix Metalloproteinase 13; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9