Imaging mouse development with confocal time-lapse microscopy.
The gene expression, signaling, and cellular dynamics driving mouse embryo development have emerged through embryology and genetic studies. However, since mouse development is a temporally regulated three-dimensional process, any insight needs to be placed in this context of real-time visualization. Live imaging using genetically encoded fluorescent protein reporters is pushing the envelope of our understanding by uncovering unprecedented insights into mouse development and leading to the formulation of quantitative accurate models.
Nowotschin
Sonja
S
Developmental Biology Program, Sloan-Kettering Institute, New York, USA.
Ferrer-Vaquer
Anna
A
Hadjantonakis
Anna-Katerina
AK
D000818:Animals; D003470:Culture Media / Q000737:chemistry; D046149:Embryo Culture Techniques / Q000295:instrumentation / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism*; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D011247:Pregnancy; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D003470:Culture Media; D005456:Fluorescent Dyes