Tumour bed irradiation of human tumour xenografts in a nude rat model using a common X-ray tube.
Studies that investigate the radiation of human tumour xenografts require an appropriate radiation source and highly standardized conditions during radiation. This work reports on the design of a standardized irradiation device using a commercially available X-ray tube with a custom constructed lead collimator with two circular apertures and an animal bed plate, permitting synchronous irradiation of two animals. Dosimetry and the corresponding methodology for radiotherapy of human non-small cell lung cancer xenograft tumours transplanted to and growing subcutaneously on the right lower limb in a nude rat model were investigated. Procedures and results described herein prove the feasibility of use of the device, which is applicable for any investigation involving irradiation of non-tumorous and tumorous lesions in small animals.
Tokalov
S V
SV
OncoRay - Centre for Radiation Research in Oncology, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, 01307 Dresden, Germany. Sergey.Tokalov@oncoray.de
Enghardt
W
W
Abolmaali
N
N
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000532:radiotherapy*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008297:Male; D011878:Radiotherapy / Q000295:instrumentation / Q000379:methods; D051381:Rats; D011923:Rats, Nude; D014965:X-Rays; D023041:Xenograft Model Antitumor Assays