Isolation and characterization of an Arthrobacter sp. strain HB-5 that transforms atrazine.
A bacterial strain (HB-5) capable of utilizing atrazine as sole carbon and nitrogen source for growth was isolated from an industrial wastewater sample by enrichment culture. The isolate was identified as Arthrobacter sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. The strain exhibited faster atrazine degradation rates in atrazine-containing mineral media than the well-characterized atrazine-degrading bacteria Pseudomonas sp. ADP. The broad optimum pH and temperature ranges observed for strain HB-5 indicate that it has potential for remediation of atrazine-contaminated sites. Strain HB-5 first metabolizes atrazine to yield hydroxyatrazine. Then, the bacterium metabolizes hydroxyatrazine to cyanuric acid, but could not mineralize atrazine.
Wang
Jinhua
J
College of Resources and Environment, Shandong Agriculture University, 271018 Taian, China.
Zhu
Lusheng
L
Liu
Aiju
A
Ma
Tingting
T
Wang
Qi
Q
Xie
Hui
H
Wang
Jun
J
Jiang
Ting
T
Zhao
Rusong
R
D001173:Arthrobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism*; D002681:China; D005798:Genes, Bacterial / Q000235:genetics; D006540:Herbicides / Q000378:metabolism; D007220:Industrial Waste; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000378:metabolism
D006540:Herbicides; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants; D002244:Carbon; D009584:Nitrogen; D001280:Atrazine