Comparison of two mice strains, A/J and C57BL/6, in caspase-1 activity and IL-1beta secretion of macrophage to Mycobacterium leprae infection.
A/J mice were found to have amino acid differences in Naip5, one of the NOD-like receptors (NLRs) involved in the cytosolic recognition of pathogen-associated molecular patterns and one of the adaptor proteins for caspase-1 activation. This defect was associated with a susceptibility to Legionella infection, suggesting an important role for Naip5 in the immune response also to other intracellular pathogens, such as Mycobacterium leprae. In this study, the immune responses of macrophages from A/J mice against M. leprae were compared to those of macrophages from C57BL/6 mice. Infection with M. leprae induced high levels of TNF-alpha production and NF-kappaB activation in A/J and C57BL/6 macrophages. Caspase-1 activation and IL-1beta secretion were also induced in both macrophages. However, macrophages from A/J mice exhibited reduced caspase-1 activation and IL-1beta secretion compared to C57BL/6 macrophages. These results suggest that NLR family proteins may have a role in the innate immune response to M. leprae.
Kang
Tae Jin
TJ
Institute of Chronic Disease, College of Pharmacy, Sahmyook University, 26-21 Kongnung 2-dong, Nowon-gu, Seoul 139-742, Republic of Korea. kangtj@syu.ac.kr
Lee
Geum Seon
GS
Kim
Se Kon
SK
Jin
Song Hou
SH
Chae
Gue Tae
GT
D000818:Animals; D020170:Caspase 1 / Q000378:metabolism*; D007107:Immune System / Q000502:physiology*; D053583:Interleukin-1beta / Q000378:metabolism*; D007918:Leprosy / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009166:Mycobacterium leprae / Q000276:immunology* / Q000472:pathogenicity; D051616:Neuronal Apoptosis-Inhibitory Protein / Q000235:genetics / Q000276:immunology; D013045:Species Specificity
D053583:Interleukin-1beta; C496806:Naip5 protein, mouse; D051616:Neuronal Apoptosis-Inhibitory Protein; D020170:Caspase 1