title: Alterations in mRNA expression of acetylcholinesterase in brain and muscle of common carp exposed to atrazine and chlorpyrifos.
abstract: The uses of pesticides and herbicides have become an integral part of modern agricultural systems. The intensive use of pesticides chlorpyrifos (CPF) and herbicides atrazine (ATR) has resulted in serious environmental problems. Herein, we have developed real-time quantitative polymerase chain reaction assays for common carp (Cyprinus carpio L.) mRNA. The levels of AChE mRNA were evaluated in brain and muscle collected from common carp by treatment of ATR, CPF, and their mixture. The decreased transcription of AChE was detected in both tissues at different doses of the toxicants in the end of exposure tests, and the changes were improved in the end of recovery tests in varying degrees. It is suggested that transcription inhibition of AChE might be significant in long-playing single or associated exposure of ATR and CPF in common carp. Alteration in transcription of AChE caused by ATR, CPF, and their mixture could reveal the toxic mechanisms related to cholinergic signaling.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors.forename: Houjuan
authors.initials: H
authors.lastname: Xing
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Jintao
authors.initials: J
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001280:Atrazine / Q000633:toxicity; D001921:Brain / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D004390:Chlorpyrifos / Q000633:toxicity; D017931:DNA Primers / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000378:metabolism; D010575:Pesticides / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018675:Toxicity Tests / Q000379:methods
chemical_list: D017931:DNA Primers; D010575:Pesticides; D012333:RNA, Messenger; D000110:Acetylcholinesterase; D004390:Chlorpyrifos; D001280:Atrazine