title: Receptor for advanced glycation endproducts mediates pro-atherogenic responses to periodontal infection in vascular endothelial cells.
abstract: OBJECTIVE
  A link between periodontal infections and an increased risk for vascular disease has been demonstrated. Porphyromonas gingivalis, a major periodontal pathogen, localizes in human atherosclerotic plaques, accelerates atherosclerosis in animal models and modulates vascular cell function. The receptor for advanced glycation endproducts (RAGE) regulates vascular inflammation and atherogenesis. We hypothesized that RAGE is involved in P. gingivalis's contribution to pro-atherogenic responses in vascular endothelial cells.
  
  
  RESULTS
  Murine aortic endothelial cells (MAEC) were isolated from wild-type C57BL/6 or RAGE-/- mice and were infected with P. gingivalis strain 381. P. gingivalis 381 infection significantly enhanced expression of RAGE in wild-type MAEC. Levels of pro-atherogenic advanced glycation endproducts (AGEs) and monocyte chemoattractant protein 1 (MCP-1) were significantly increased in wild-type MAEC following P. gingivalis 381 infection, but were unaffected in MAEC from RAGE-/- mice or in MAEC infected with DPG3, a fimbriae-deficient mutant of P. gingivalis 381. Consistent with a role for oxidative stress and an AGE-dependent activation of RAGE in this setting, both antioxidant treatment and AGE blockade significantly suppressed RAGE gene expression and RAGE and MCP-1 protein levels in P. gingivalis 381-infected human aortic endothelial cells (HAEC).
  
  
  CONCLUSIONS
  The present findings implicate for the first time the AGE-RAGE axis in the amplification of pro-atherogenic responses triggered by P. gingivalis in vascular endothelial cells.
authors.affiliation: Division of Periodontics, College of Dental Medicine, Columbia University, 630 W. 168th Street, PH7E-110, New York, NY, United States.
authors.forename: Andreas
authors.initials: A
authors.lastname: Pollreisz
authors.forename: Barry I
authors.initials: BI
authors.lastname: Hudson
authors.forename: Jong S
authors.initials: JS
authors.lastname: Chang
authors.forename: Wu
authors.initials: W
authors.lastname: Qu
authors.forename: Bin
authors.initials: B
authors.lastname: Cheng
authors.forename: Panos N
authors.initials: PN
authors.lastname: Papapanou
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Schmidt
authors.forename: Evanthia
authors.initials: E
authors.lastname: Lalla
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D050197:Atherosclerosis / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D000939:Epitopes; D006801:Humans; D007249:Inflammation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D010518:Periodontitis / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000939:Epitopes; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic