title: Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex.
abstract: The Golgi-associated retrograde protein (GARP) complex mediates tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). In the yeast Saccharomyces cerevisiae, GARP comprises four subunits named Vps51p, Vps52p, Vps53p, and Vps54p. Orthologues of the GARP subunits, except for Vps51p, have been identified in all other eukaryotes. A yeast two-hybrid screen of a human cDNA library yielded a phylogenetically conserved protein, Ang2/Fat-free, which interacts with human Vps52, Vps53 and Vps54. Human Ang2 is larger than yeast Vps51p, but exhibits significant homology in an N-terminal coiled-coil region that mediates assembly with other GARP subunits. Biochemical analyses show that human Ang2, Vps52, Vps53 and Vps54 form an obligatory 1:1:1:1 complex that strongly interacts with the regulatory Habc domain of the TGN SNARE, Syntaxin 6. Depletion of Ang2 or the GARP subunits similarly impairs protein retrieval to the TGN, lysosomal enzyme sorting, endosomal cholesterol traffic¤ and autophagy. These findings indicate that Ang2 is the missing component of the GARP complex in most eukaryotes.
authors.affiliation: Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Pérez-Victoria
authors.forename: Christina
authors.initials: C
authors.lastname: Schindler
authors.forename: Javier G
authors.initials: JG
authors.lastname: Magadán
authors.forename: Gonzalo A
authors.initials: GA
authors.lastname: Mardones
authors.forename: Cédric
authors.initials: C
authors.lastname: Delevoye
authors.forename: Maryse
authors.initials: M
authors.lastname: Romao
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
authors.forename: Juan S
authors.initials: JS
authors.lastname: Bonifacino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy; D002784:Cholesterol / Q000378:metabolism; D017124:Conserved Sequence; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport; D050765:Qa-SNARE Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D040681:Structural Homology, Protein; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000172:deficiency / Q000378:metabolism*; D015027:Zebrafish; D021601:trans-Golgi Network / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D050765:Qa-SNARE Proteins; C555023:VPS51 protein, human; D033921:Vesicular Transport Proteins; D002784:Cholesterol