Cervical collagen and biomechanical strength in non-pregnant women with a history of cervical insufficiency.
BACKGROUND
It has been suggested that cervical insufficiency (CI) is characterized by a "muscular cervix" with low collagen and high smooth muscle concentrations also in the non-pregnant state. Therefore, the aim of this study was to investigate the biomechanical properties, collagen concentration, smooth muscle cell density, and collagen fiber orientation in cervical biopsies from non-pregnant women with a history of CI.


METHODS
Cervical punch biopsies (2 x 15 mm) were obtained from 57 normal non-pregnant women and 22 women with a history of CI. Biomechanical tensile testing was performed, and collagen content was determined by hydroxyproline quantification. Histomorphometry was used to determine the volume densities of extracellular matrix and smooth muscle cells from the distal to the proximal part of each sample. Smooth muscle cells were identified using immunohistochemistry. Finally, collagen fiber orientation was investigated. Data are given as mean +/- SD.


RESULTS
Collagen concentration was lower in the CI group (58.6 +/- 8.8%) compared with the control group (62.2 +/- 6.6%) (p = 0.033). However, when data were adjusted for age and parity, no difference in collagen concentration was found between the two groups. Maximum load of the specimens did not differ between the groups (p = 0.78). The tensile strength of cervical collagen, i.e. maximum load normalized per unit collagen (mg of collagen per mm of specimen length), was increased in the CI group compared with controls (p = 0.033). No differences in the volume density of extracellular matrix or smooth muscle cells were found between the two groups. Fibers not oriented in the plane of sectioning were increased in CI patients compared with controls.


CONCLUSIONS
Cervical insufficiency does not appear to be associated with a constitutionally low collagen concentration or collagen of inferior mechanical quality. Furthermore, the hypothesis that a "muscular cervix" with an abundance of smooth muscle cells contributes to the development of cervical insufficiency is not supported by the present study.
Oxlund
Birgitte S
BS
Department of Obstetrics and Gynecology, Aarhus University Hospital, Skejby, DK-8200 Aarhus N, Denmark. boxlund@dadlnet.dk
Ørtoft
Gitte
G
Brüel
Annemarie
A
Danielsen
Carl Christian
CC
Oxlund
Hans
H
Uldbjerg
Niels
N
D000328:Adult; D001696:Biomechanical Phenomena / Q000502:physiology; D001706:Biopsy; D016764:Cell Polarity / Q000502:physiology; D002584:Cervix Uteri / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D053580:Muscle Strength / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D011247:Pregnancy; D017584:Reproductive History; D014018:Tissue Distribution; D002581:Uterine Cervical Incompetence / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
D003094:Collagen