Overexpression of the adeB gene in clinical isolates of tigecycline-nonsusceptible Acinetobacter baumannii without insertion mutations in adeRS.
Thirteen clinical isolates of multidrug-resistant Acinetobacter baumannii resistant to carbapenems (MRAB-C) with tigecycline nonsusceptibility were collected from individual patients in this study. None of the 13 isolates shared the same strain characteristics in molecular typing. All of them showed increased adeB transcription, as predicted. However, none of these tigecycline-nonsusceptible MRAB-C isolates were found to possess previously known adeRS mutations. Upregulation of adeB transcription may result from cross stimulation by other mechanisms.
Sun
Jun-Ren
JR
Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, 325 Section 2 Cheng-Kung Road, Neihu, Taipei, Taiwan.
Chan
Ming-Chin
MC
Chang
Tein-Yao
TY
Wang
Wei-Yao
WY
Chiueh
Tzong-Shi
TS
D000151:Acinetobacter Infections / Q000188:drug therapy; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D008875:Middle Aged; D008911:Minocycline / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000078304:Tigecycline
C445684:AdeB protein, Acinetobacter baumannii; D001426:Bacterial Proteins; D015780:Carbapenems; D026901:Membrane Transport Proteins; D000078304:Tigecycline; D008911:Minocycline