Preparation of mouse brain tissue for immunoelectron microscopy.
Transmission electron microscopy (TEM) is extremely useful for visualizing microglial, oligodendrocytic, astrocytic, and neuronal subcellular compartments (dendrite, dendritic spine, axon, axon terminal, perikaryon), as well as their intracellular organelles and cytoskeleton, in the central nervous system at high spatial resolution. Combined with TEM, pre-embedding immunocytochemistry allows the discrimination of cellular elements with few distinctive features and identification criteria (e.g., microglial perikarya and processes, when using an antibody against the microglia-specific marker Iba1 (ionized calcium binding adaptor molecule 1; as presented here)), identifying the neurotransmitter contents of cellular elements (e.g., serotonergic) and their ultrastructural localization of soluble or membrane-bound proteins (e.g., 5 HT1A and EphA4 receptors). Here, we describe a protocol for transcardiac perfusion of mice with acrolein fixative, removal and sectioning of the brain, as well as immunoperoxidase-diaminobenzidine (DAB) staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with TEM. When rigorously performed, this technique provides an excellent compromise between optimal ultrastructural preservation and immunocytochemical detection.
Tremblay
Marie-Eve
ME
Department of Neurobiology and Anatomy, University of Rochester, NY, USA. Marie-Eve_Tremblay@URMC.rochester.edu
Riad
Mustapha
M
Majewska
Ania
A
D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron / Q000379:methods*