The chemical constituents of Piper kadsura and their cytotoxic and anti-neuroinflammtaory activities.
The n-hexane and CHCl₃ soluble fractions of the MeOH extract of the aerial parts of Piper kadsura were found to potently inhibit nitric oxide (NO) production in LPS-activated BV-2 cells, a microglial cell line. From the active fractions, a new stereoisomer of guaiane sesquiterpene, 1α,5β-guai-4(15)-ene-6β,10β-diol, kadsuguain A (1) and a new cyclohexadienone, kadsuketanone A (2), together with twelve known compounds (3-14) were isolated. The structures of these compounds were elucidated by extensive NMR spectral studies. The absolute configuration of 2 was determined by circular dichroism (CD) spectra. Compounds 2, 6, and 11-14 significantly inhibited both nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in the LPS-activated microglia cells. In addition, compounds 4, 6, and 11-14 exhibited cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT15 human tumour cells.
Kim
Ki Hyun
KH
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, Korea.
Choi
Jung Wook
JW
Choi
Sang Un
SU
Ha
Sang Keun
SK
Kim
Sun Yeou
SY
Park
Hee-Juhn
HJ
Lee
Kang Ro
KR
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D015213:Neuroimmunomodulation / Q000187:drug effects*; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry
D000970:Antineoplastic Agents; D010936:Plant Extracts