title: Phenilpropanoate identification in young wheat plants by liquid chromatography/tandem mass spectrometry: monomeric and dimeric compounds.
abstract: Metabolomic approach has become a very important tool for determining the actual gene expression and protein activity. Metabolism via the shikimate pathway in plant gives rise to a large number of aromatic compounds, including p-hydroxycinnamates, which are mainly associated with their cell walls in complex structures, ester-linked to heteroxilans. In this work, ferulate dehydrodimers and related compounds were extracted from the cell walls of young lyophilized wheat plants with 2 mol/l NaOH for 4 h at 25 °C under N(2). After solid phase extraction, samples were analysed by high-performance liquid chromatography-quadrupole-linear ion trap tandem mass spectrometry with the electrospray source operating in negative mode. Nineteen dehydrodiferulates, 2 hydrate forms, 3 oxo- forms, 2 decarboxilated forms and 1 dihydroxysinapate, were identified by mass spectra interpretation. In addition, five mixed dehydrodimers of ferulic-sinapic, ferulic-coumaric and ferulic-syringic acids were also identified. Reproducibility of peak areas was usually better than 10% but some minor components which may be procedural artefacts, although relatively mild conditions were used.
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Callipo
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.forename: Valentina
authors.initials: V
authors.lastname: Fuscoletti
authors.forename: Riccardo
authors.initials: R
authors.lastname: Gubbiotti
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000737:chemistry*; D010666:Phenylpropionates / Q000737:chemistry*; D015203:Reproducibility of Results; D012765:Shikimic Acid / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry*
chemical_list: D003373:Coumaric Acids; D010666:Phenylpropionates; D012765:Shikimic Acid; C004999:ferulic acid; C520807:dihydroferulic acid