title: Subnuclear localization and intranuclear trafficking of transcription factors.
abstract: Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
authors.affiliation: Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Sayyed K
authors.initials: SK
authors.lastname: Zaidi
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Medina
authors.forename: Shirwin M
authors.initials: SM
authors.lastname: Pockwinse
authors.forename: Rachit
authors.initials: R
authors.lastname: Bakshi
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Kota
authors.forename: Syed A
authors.initials: SA
authors.lastname: Ali
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Young
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Nickerson
authors.forename: Amjad
authors.initials: A
authors.lastname: Javed
authors.forename: Martin
authors.initials: M
authors.lastname: Montecino
authors.forename: Andre J
authors.initials: AJ
authors.lastname: van Wijnen
authors.forename: Jane B
authors.initials: JB
authors.lastname: Lian
authors.forename: Janet L
authors.initials: JL
authors.lastname: Stein
authors.forename: Gary S
authors.initials: GS
authors.lastname: Stein
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D019295:Computational Biology; D036681:Fluorescence Recovery After Photobleaching; D007382:Intermediate Filaments / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D042581:Intranuclear Space / Q000378:metabolism*; D008677:Metaphase; D008853:Microscopy; D015530:Nuclear Matrix / Q000378:metabolism; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors