Adsorption from saliva to silica and hydroxyapatite surfaces and elution of salivary films by SDS and delmopinol.
The adsorption of proteins from human whole saliva (HWS) onto silica and hydroxyapatite surfaces (HA) was followed by quartz crystal microbalance with dissipation (QCM-D) and ellipsometry. The influence of different surface properties and adsorption media (water and PBS) on the adsorption from saliva was studied. The viscoelastic properties of the salivary films formed on the solid surfaces were estimated by the use of the Voigt-based viscoelastic film model. Furthermore, the efficiency of SDS and delmopinol to elute the adsorbed salivary film from the surfaces was investigated at different surfactant concentrations. A biphasic kinetic regime for the adsorption from saliva on the silica and HA surfaces was observed, indicating the formation of a rigidly coupled first layer corresponding to an initial adsorption of small proteins and a more loosely bound second layer. The results further showed a higher adsorption from HWS onto the HA surfaces compared to the silica surfaces in both adsorption media (PBS and water). The adsorption in PBS led to higher adsorbed amounts on both surfaces as compared to water. SDS was found to be more efficient in removing the salivary film from both surfaces than delmopinol. The salivary film was found to be less tightly bound onto the silica surfaces since more of the salivary film could be removed with both SDS and delmopinol compared to that from the HA surface. When adsorption took place from PBS the salivary layer formed at both surfaces seemed to have a similar structure, with a high energy dissipation implying that a softer salivary layer is built up in PBS as opposed to that in water. Furthermore, the salivary layers adsorbed from water solutions onto the HA were found to be softer than those on silica.
Santos
Olga
O
Biomedical Laboratory Science & Biomedical Technology, Faculty of Health and Society, Malmo University, Malmo, Sweden. olga.santos@mah.se
Lindh
Liselott
L
Halthur
Tobias
T
Arnebrant
Thomas
T
D000327:Adsorption; D000328:Adult; D018441:Biofilms; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D009025:Morpholines / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D009025:Morpholines; D012471:Salivary Proteins and Peptides; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D012822:Silicon Dioxide; D017886:Durapatite; C073738:delmopinol