Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene.
This paper describes the application of plasmonics-based nanoprobes that combine the modulation of the plasmonics effect to change the surface-enhanced Raman scattering (SERS) of a Raman label and the specificity of a DNA hairpin loop sequence to recognize and discriminate a variety of molecular target sequences. Hybridization with target DNA opens the hairpin and physically separates the Raman label from the metal nanoparticle thus reducing the plasmonics effect and quenching the SERS signal of the label. We have successfully demonstrated the specificity and selectivity of the nanoprobes in the detection of a single-nucleotide polymorphism (SNP) in the breast cancer BRCA1 gene in a homogenous solution at room temperature. In addition, the potential application of plasmonics nanoprobes for quantitative DNA diagnostic testing is discussed.
Wabuyele
Musundi B
MB
GlaxoSmithKline, 1250 South Collegeville Road, UP12-210, P.O. Box 5089, Collegeville, PA 19426, USA.
Yan
Fei
F
Vo-Dinh
Tuan
T
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D001943:Breast Neoplasms / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*
D004247:DNA