Structure monomorphism of RNA G-quadruplex that is independent of surrounding condition.
G-quadruplex structures in nucleic acids have become attractive targets of study because their distinct structural forms may have diverse biological functions. Here, we preliminary investigated the structure and thermodynamics of G-quadruplexes of RNAs and corresponding DNAs of the same sequence by CD, UV and PAGE. Structural analysis demonstrated that the RNA sequences folded into a parallel-stranded G-quadruplex independently of surrounding conditions with different cations (K(+) and Na(+)) under both dilute and molecular crowding conditions. In contrast, G-quadruplexes of their corresponding DNA sequences showed structural polymorphism depending on the surrounding conditions. Moreover, it was demonstrated that parallel G-quadruplexes of the RNA sequences were more stable than either parallel or antiparallel G-quadruplexes of the same DNA sequences. These results allow us to propose a single, robust RNA G-quadruplex structure that exists under various conditions.
Zhang
Dong-Hao
DH
College of Pharmacy, Hebei University, Wusidong Road 180#, Baoding, 071002, China. zhangdonghao1@gmail.com
Zhi
Gao-Ying
GY
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D054856:G-Quadruplexes; D019254:Genes, bcl-2; D006801:Humans; D009690:Nucleic Acid Conformation; D011189:Potassium Chloride; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D013816:Thermodynamics
D012313:RNA; D011189:Potassium Chloride; D004247:DNA