title: The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea.
abstract: The mutable a(flaked (a(f)) allele at the A locus of the common morning glory (Ipomoea purpurea) confers incomplete dominance in flower pigmentation and is caused by insertion of the DNA transposon Tip100 into CHS-D, which encodes chalcone synthase and is required for anthocyanin biosynthesis. Levels of CHS-D transcripts, CHS-D protein, and anthocyanin pigment in heterozygous flowers were about half that in homozygous flowers, indicating that dosage-dependent expression of CHS-D is the primary cause of the observed incomplete dominance. This contrasts with the Nivea locus in snapdragon (Antirrhinum majus) in which incomplete dominance is caused by semi-dominant CHS alleles.
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-Ku, Shizuoka 422-8526, Japan. yasuyo@u-shizuoka-ken.ac.jp
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Johzuka-Hisatomi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Iida
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000483:Alleles; D000872:Anthocyanins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics; D018628:Gene Dosage; D017343:Genes, Plant / Q000235:genetics; D027741:Ipomoea / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010088:Oxidoreductases / Q000235:genetics; D010858:Pigmentation / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D000872:Anthocyanins; D004251:DNA Transposable Elements; D018744:DNA, Plant; D010088:Oxidoreductases; D000217:Acyltransferases; C020141:flavanone synthetase