title: Fine mapping of the sunflower resistance locus Pl(ARG) introduced from the wild species Helianthus argophyllus.
abstract: Downy mildew, caused by Plasmopara halstedii, is one of the most destructive diseases in cultivated sunflower (Helianthus annuus L.). The dominant resistance locus Pl(ARG) originates from silverleaf sunflower (H. argophyllus Torrey and Gray) and confers resistance to all known races of P. halstedii. We mapped Pl(ARG) on linkage group (LG) 1 of (cms)HA342 × ARG1575-2, a population consisting of 2,145 F(2) individuals. Further, we identified resistance gene candidates (RGCs) that cosegregated with Pl(ARG) as well as closely linked flanking markers. Markers from the target region were mapped with higher resolution in NDBLOS(sel) × KWS04, a population consisting of 2,780 F(2) individuals that does not segregate for Pl(ARG). A large-insert sunflower bacterial artificial chromosome (BAC) library was screened with overgo probes designed for markers RGC52 and RGC151, which cosegregated with Pl(ARG). Two RGC-containing BAC contigs were anchored to the Pl(ARG) region on LG 1.
authors.affiliation: Plant Breeding, Center of Life and Food Sciences Weihenstephan, Technische Universität München, 85354, Freising, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wieckhorst
authors.forename: E
authors.initials: E
authors.lastname: Bachlava
authors.forename: C M
authors.initials: CM
authors.lastname: Dussle
authors.forename: S
authors.initials: S
authors.lastname: Tang
authors.forename: W
authors.initials: W
authors.lastname: Gao
authors.forename: C
authors.initials: C
authors.lastname: Saski
authors.forename: S J
authors.initials: SJ
authors.lastname: Knapp
authors.forename: C-C
authors.initials: CC
authors.lastname: Schön
authors.forename: V
authors.initials: V
authors.lastname: Hahn
authors.forename: E
authors.initials: E
authors.lastname: Bauer
mesh_terms: D001483:Base Sequence; D020090:Chromosome Segregation / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003433:Crosses, Genetic; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D056426:Genetic Loci / Q000235:genetics*; D005819:Genetic Markers; D005820:Genetic Testing; D005828:Genetics, Population; D006239:Haplotypes / Q000235:genetics; D006368:Helianthus / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D007113:Immunity, Innate / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics; D044742:Peronospora / Q000502:physiology*; D020161:Physical Chromosome Mapping / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D005819:Genetic Markers