Optimization of selected cultivation parameters for Cordyceps guangdongensis.
OBJECTIVE
To increase the fruit body production of Cordyceps guangdongensis, selected cultivation conditions, especially nutritional parameters were optimized.


RESULTS
Cordyceps guangdongensis was inoculated on potato dextrose agar slants with pH values from 4.5 to 9.0 and cultivated in artificial media with different carbon and nitrogen supplements. Primordium formation in C. guangdongensis was favoured by slightly acidic conditions. Fruit body yields and biological efficiency (BE) recorded were all highest in cultures of C. guangdongensis supplemented with sucrose and KNO3 as carbon and nitrogen supplements, respectively. Highest fruit body yields and BE values were recorded with C : N ratio of 12 : 1. The optimal medium consisted of (g l(-1)) 20.0 sucrose, 4.0 soya bean powder, 5.0 beef extract and 10.0 KNO3. Cultivation experiments using this medium confirmed its reliability; 18.35% of BE was obtained, compared with a calculated maximum BE of 18.65% based on orthogonal test data.


CONCLUSIONS
Cordyceps guangdongensis preferred sucrose and potassium nitrate as best carbon and nitrogen supplements. It produced satisfying yield of fruit body with optimized medium.


CONCLUSIONS
Optimized artificial cultivation conditions could promote the yield of C. guangdongensis and decreased the cost of production.
Lin
Q Y
QY
Guangdong Provincial Open Laboratory of New Microbial Application Technology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, China.
Song
B
B
Huang
H
H
Li
T H
TH
D002244:Carbon / Q000378:metabolism; D032661:Cordyceps / Q000166:cytology* / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009172:Mycology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism
D003470:Culture Media; D002244:Carbon; D009584:Nitrogen