Incorrect DNA methylation of the DAZL promoter CpG island associates with defective human sperm.
BACKGROUND
Successful gametogenesis requires the establishment of an appropriate epigenetic state in developing germ cells. Nevertheless, an association between abnormal spermatogenesis and epigenetic disturbances in germline-specific genes remains to be demonstrated.


METHODS
In this study, the DNA methylation pattern of the promoter CpG island (CGI) of two germline regulator genes--DAZL and DAZ, was characterized by bisulphite genomic sequencing in quality-fractioned ejaculated sperm populations from normozoospermic (NZ) and oligoasthenoteratozoospermic (OAT) men.


RESULTS
OAT patients display increased methylation defects in the DAZL promoter CGI when compared with NZ controls. Such differences are recorded when analyzing sperm fractions enriched either in normal or defective germ cells (P< 0.001 in both cases). Significant differences in DNA methylation profiles are also observable when comparing the qualitatively distinct germ cell fractions inside the NZ and OAT groups (P= 0.003 and P= 0.007, respectively). Contrastingly, the unmethylation pattern of the DAZ promoter CGI remains correctly established in all experimental groups.


CONCLUSIONS
An association between disrupted DNA methylation of a key spermatogenesis gene and abnormal human sperm is described here for the first time. These results suggest that incorrect epigenetic marks in germline genes may be correlated with male gametogenic defects.
Navarro-Costa
Paulo
P
Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, 1649-028 Lisboa, Portugal. navarro-costa@fm.ul.pt
Nogueira
Paulo
P
Carvalho
Marta
M
Leal
Fernanda
F
Cordeiro
Isabel
I
Calhaz-Jorge
Carlos
C
Gonçalves
João
J
Plancha
Carlos E
CE
D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics; D018899:CpG Islands; D019175:DNA Methylation; D000075364:Deleted in Azoospermia 1 Protein; D057890:Epigenomics; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000473:pathology*
C095668:DAZ1 protein, human; C101358:DAZL protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins