title: Quantitation in bioluminescence imaging by correction of tissue absorption for experimental oncology.
abstract: OBJECTIVE
  We have developed a method of quantitation for correcting tissue absorption in in vivo bioluminescence imaging (BLI).
  
  
  METHODS
  Variations of luciferin emission spectrum were determined and were related to photon absorption to determine a correction curve. This was validated by combining BLI with tomoscintigraphy and tomodensitometry, which were applied to a lymphoma model.
  
  
  RESULTS
  Tissue absorption affects luciferin emission spectrum, mainly for wavelengths less than 620 nm. So, we have selected two filters bordering 620 nm to quantify spectral modifications. A significant correlation was obtained between the spectral analysis and the percentage of transmitted light through tissues (R(2) = 0.97). On a disseminated tumour model, we have shown that such a methodology is of great interest to compare bioluminescent signals and to get more accurate quantitative data about tumour proliferation.
  
  
  CONCLUSIONS
  Spectral analysis allows improved quantitation of BLI and could be of value to perform pharmacological studies and to follow tumour progression in models with tumours evolving in different locations.
authors.affiliation: UPS no 44 TAAM-CIPA, CNRS, Orléans, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Pesnel
authors.forename: Arnaud
authors.initials: A
authors.lastname: Pillon
authors.forename: Laurent
authors.initials: L
authors.lastname: Créancier
authors.forename: Nicolas
authors.initials: N
authors.lastname: Guilbaud
authors.forename: Christian
authors.initials: C
authors.lastname: Bailly
authors.forename: Anna
authors.initials: A
authors.lastname: Kruczynski
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Lerondel
authors.forename: Alain
authors.initials: A
authors.lastname: Le Pape
mesh_terms: D000042:Absorption; D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D049454:Firefly Luciferin / Q000378:metabolism; D006801:Humans; D008027:Light; D008163:Luminescent Measurements / Q000379:methods*; D008223:Lymphoma / Q000175:diagnosis; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D009928:Organ Specificity; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D049454:Firefly Luciferin