title: Reverse phase protein microarray technology in traumatic brain injury.
abstract: Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology can address problematic issues, such as sample complexity, sensitivity, quantification, reproducibility and throughput, which are currently associated with mass spectrometry-based approaches. However, there are technical challenges, predominantly associated with the selection and use of antibodies, preparation and representation of samples and with analyzing and quantifying primary RPPA data. Here we present ways to identify and overcome some of the current issues associated with RPPA. We believe that using stringent quality controls, improved bioinformatics analysis and interpretation of primary RPPA data, this method will significantly contribute in generating new level of understanding about complex disorders at the level of systems biology.
authors.affiliation: Department of Anatomy, Physiology and Genetics, School of Medicine, Uniformed Services University of Health Sciences, Bethesda, MD 20814, USA. agyorgy@usuhs.mil
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Gyorgy
authors.forename: John
authors.initials: J
authors.lastname: Walker
authors.forename: Dan
authors.initials: D
authors.lastname: Wingo
authors.forename: Ofer
authors.initials: O
authors.lastname: Eidelman
authors.forename: Harvey B
authors.initials: HB
authors.lastname: Pollard
authors.forename: Andras
authors.initials: A
authors.lastname: Molnar
authors.forename: Denes V
authors.initials: DV
authors.lastname: Agoston
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000473:pathology; D053179:Caspase 7 / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D046228:Microarray Analysis / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D051381:Rats; D013552:Swine
chemical_list: D009419:Nerve Tissue Proteins; D053179:Caspase 7