Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.
A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.
Jung
Suk-Chae
SC
School of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
Smith
Chris L
CL
Lee
Ki-Sung
KS
Hong
Min-Eui
ME
Kweon
Dae-Hyuk
DH
Stephanopoulos
Gregory
G
Jin
Yong-Su
YS
D000264:Adenylosuccinate Lyase / Q000235:genetics*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D005816:Genetic Complementation Test; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D017354:Point Mutation / Q000235:genetics
D003470:Culture Media; D000264:Adenylosuccinate Lyase