One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules.
A simple method for a chemical surface modification of poly(methyl methacrylate) (PMMA) microchips with amino-poly(ethyleneglycol) (PEG-NH(2)) by nucleophilic addition-elimination reaction was developed to improve the separation efficiency and analytical reproducibility in a microchip electrophoresis (MCE) analysis of biomolecules such as proteins and enantiomers. In our procedure, the PEG chains were robustly immobilized only by introducing an aqueous solution of PEG-NH(2) into the PMMA microchannel. The electroosmotic mobilities on the modified chips remained almost constant during 35 days with 37 runs without any recoating. The PEG-NH(2) modified chip provided a fast, reproducible, efficient MCE separation of proteins with a wide variety of isoelectric points within 15s. Furthermore, the application of the modified chip to affinity electrophoresis using bovine serum albumin gave a good chiral separation of amino acids.
Kitagawa
Fumihiko
F
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. fkitagawa@anchem.mc.kyoto-u.ac.jp
Kubota
Kei
K
Sueyoshi
Kenji
K
Otsuka
Koji
K
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods; D046209:Electrophoresis, Microchip / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods*; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000032:analysis* / Q000737:chemistry
D000596:Amino Acids; D011092:Polyethylene Glycols; D019904:Polymethyl Methacrylate