title: Apoptotic effect of quercetin on HT-29 colon cancer cells via the AMPK signaling pathway.
abstract: Activation of AMP-activated protein kinase (AMPK), a physiological cellular energy sensor, strongly suppresses cell proliferation in both nonmalignant and tumor cells. This study demonstrates the mechanism of quercetin-induced apoptosis in HT-29 colon cancer cells. Treatment of cells with quercetin significantly decreased cell viability in a dose-dependent manner. Notably, quercetin increased cell cycle arrest in the G1 phase and up-regulated apoptosis-related proteins, such as AMPK, p53, and p21, within 48 h. Furthermore, in vivo experiments showed that quercetin treatment resulted in a significant reduction in tumor volume over 6 weeks, and apoptosis-related protein induction by quercetin was significantly higher in the 100 mg/kg treated group compared to the control group. All of these results indicate that quercetin induces apoptosis via AMPK activation and p53-dependent apoptotic cell death in HT-29 colon cancer cells and that it may be a potential chemopreventive or therapeutic agent against HT-29 colon cancer.
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju National University, 340-702 Yesan, Republic of Korea.
authors.forename: Hyeong-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Byeong-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Park
authors.forename: Byung-Kwon
authors.initials: BK
authors.lastname: Park
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Changsun
authors.initials: C
authors.lastname: Choi
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Jung
authors.forename: Kyong-Hwan
authors.initials: KH
authors.lastname: Roh
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Jung
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000503:physiopathology*; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D011794:Quercetin; D055372:AMP-Activated Protein Kinases