title: Microcapsules of alginate/chitosan containing magnetic nanoparticles for controlled release of insulin.
abstract: The challenge of this work was to investigate the potential of alginate/chitosan beads containing magnetite nanoparticles as a drug delivery system. The insulin beads were prepared by dripping a solution of sodium alginate containing insulin into a CaCl(2) solution. Magnetite nanoparticles of 5 nm mean size were synthesized inside the alginate egg-box structure by co-precipitation of Fe(III) and Fe(II) in the presence of NH(4)OH. Quantitative analysis revealed that insulin encapsulation depends on the initial protein content and 35% of insulin was entrapped by alginate beads for a protein concentration of 10 wt%. It was verified that approximately 50% of the insulin was released to Milli-Q water in 800 h release experiments. The application of oscillating magnetic field increased three fold the insulin release. The results suggest that the alginate/chitosan system containing magnetite nanoparticles is a promising system for clinical applications of controlled release of insulin in the presence of an oscillating magnetic field in a subcutaneous implant approach.
authors.affiliation: Laboratório de Nanotecnologia Biofuncional, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Priscilla Vanessa
authors.initials: PV
authors.lastname: Finotelli
authors.forename: Daniel
authors.initials: D
authors.lastname: Da Silva
authors.forename: Mauro
authors.initials: M
authors.lastname: Sola-Penna
authors.forename: Alexandre Malta
authors.initials: AM
authors.lastname: Rossi
authors.forename: Marcos
authors.initials: M
authors.lastname: Farina
authors.forename: Leonardo Rodrigues
authors.initials: LR
authors.lastname: Andrade
authors.forename: Armando Yoshihaki
authors.initials: AY
authors.lastname: Takeuchi
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Rocha-Leão
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002214:Capsules; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems / Q000379:methods; D004343:Drug Implants; D004574:Electromagnetic Fields; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010316:Particle Size
chemical_list: D000464:Alginates; D001786:Blood Glucose; D002214:Capsules; D003692:Delayed-Action Preparations; D004343:Drug Implants; D006603:Hexuronic Acids; D007004:Hypoglycemic Agents; D007328:Insulin; D058185:Magnetite Nanoparticles; D020723:Glucuronic Acid; D048271:Chitosan