Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells.
Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of α-tubulin with β-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with α-tubulin and β-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of α-tubulin and β-tubulin, increased α-tubulin and β-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.
Tai
Cheng-Jeng
CJ
Section of Hematology-Oncology, Taipei Medical University and Hospital, Taipei, Taiwan.
Shen
Shing-Chuan
SC
Lee
Woan-Ruoh
WR
Liao
Ching-Fong
CF
Deng
Win-Ping
WP
Chiou
Hung-Yi
HY
Hsieh
Cheng-I
CI
Tung
Jai-Nien
JN
Chen
Ching-Shyang
CS
Chiou
Jeng-Fong
JF
Li
Li-Tzu
LT
Lin
Chuang-Yu
CY
Hsu
Chung-Huei
CH
Jiang
Ming-Chung
MC
D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D022081:Cell Surface Extensions; D029563:Cellular Apoptosis Susceptibility Protein / Q000235:genetics / Q000502:physiology*; D005260:Female; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D010766:Phosphorylation; D014404:Tubulin / Q000378:metabolism
D029563:Cellular Apoptosis Susceptibility Protein; D014404:Tubulin