Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement.
A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R(2)=0.999) for Con A concentration from 0.084 μg/mL to 50.0 μg/mL with a remarkable detection limit (S/N=3) of 0.070 μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.
Min
In-Hong
IH
Department of Chemistry and Center for Bioactive Molecular Hybrids, Yonsei University, 134 Shinchon-dong, Seodaemoon-Gu, Seoul 120-749, Republic of Korea.
Choi
Lee
L
Ahn
Kwang-Soo
KS
Kim
Byung Kun
BK
Lee
Bo Young
BY
Kim
Kwan Soo
KS
Choi
Han Nim
HN
Lee
Won-Yong
WY
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D003124:Colorimetry; D003208:Concanavalin A / Q000032:analysis; D055664:Electrochemical Techniques; D006046:Gold; D037102:Lectins / Q000032:analysis*; D008358:Mannose; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D012834:Silver
D037102:Lectins; D003208:Concanavalin A; D012834:Silver; D006046:Gold; D008358:Mannose