title: Up-regulation of angiotensin-converting enzyme and angiotensin II type 1 receptor in irradiated rats.
abstract: OBJECTIVE
  To investigate changes in cardiac functional parameters and the cardiac expression of angiotensin-converting enzyme (ACE), angiotensin II type 1 receptor (AT1), procollagen type I (proc-I) and transforming growth factor-ß1 (TGF-ß1) in rats irradiated at heart.
  
  
  METHODS
  Male Wistar rats were irradiated with a single dose of radiation (0, 5, 10 and 15 Gray [Gy]) delivered directly to the heart and the molecular evaluations were performed at various times post-irradiation (two days, 15 days and four months). The expression of ACE, AT1, proc-I and TGF-ß1 were analysed using Real Time-Polymerase Chain Reaction (RT-PCR) and/or Western blotting. Cardiac structural and functional alterations were investigated at the four-month time point by echocardiography and by quantitative methods (stereology).
  
  
  RESULTS
  Rats irradiated with 15 Gy showed a modest reduction in the ejection fraction. Cardiac proc-I, TGF-ß1, ACE and AT1 were also measurably increased.
  
  
  CONCLUSIONS
  Irradiated rat hearts show simultaneous elevations in renin-angiotensin system components AT1 and ACE and cardiac remodeling markers proc-I and TGF-ß1.
authors.affiliation: Departamento de Biologia Geral – GBG, UFF, Niterói, Rio de Janeiro, Brazil. samararossatti@gmail.com
authors.forename: Samara Cristina
authors.initials: SC
authors.lastname: Ferreira-Machado
authors.forename: Nazareth De Novaes
authors.initials: Nde N
authors.lastname: Rocha
authors.forename: André Luiz
authors.initials: AL
authors.lastname: Mencalha
authors.forename: Luiz Dione Barbosa
authors.initials: LD
authors.lastname: De Melo
authors.forename: Camila
authors.initials: C
authors.lastname: Salata
authors.forename: Andréia Fortes
authors.initials: AF
authors.lastname: Ribeiro
authors.forename: Thiago Da Silva
authors.initials: Tda S
authors.lastname: Torres
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Mandarim-De-Lacerda
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Canary
authors.forename: Antônio Augusto De Freitas
authors.initials: AA
authors.lastname: Peregrino
authors.forename: Luís Alexandre Gonçalves
authors.initials: LA
authors.lastname: Magalhães
authors.forename: Januário Bispo
authors.initials: JB
authors.lastname: Cabral-Neto
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Dealmeida
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D006321:Heart / Q000502:physiology / Q000528:radiation effects; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000528:radiation effects; D007703:Peptidyl-Dipeptidase A / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics* / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000528:radiation effects; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000528:radiation effects*
chemical_list: D024042:Collagen Type I; D044140:Receptor, Angiotensin, Type 1; D053773:Transforming Growth Factor beta1; D007703:Peptidyl-Dipeptidase A