Lanostane triterpenes from Ganoderma lucidum suppress the adipogenesis in 3T3-L1 cells through down-regulation of SREBP-1c.
Several lanostane triterpenes [butyl ganoderate A (1), butyl ganoderate B (2), butyl lucidenate N (3), and butyl lucidenate A (4)] bearing a butyl ester side chain from the fruiting bodies of Ganoderma lucidum exhibited considerable inhibitory effects on adipogenesis in 3T3-L1 cells. The inhibitory mechanism of 1 and 3 on adipogenesis in 3T3-L1 cells was investigated; we found that the mRNA and protein expression levels of SREBP-1c were reduced by treatment with 1 and 3 versus the untreated control. Furthermore, compounds 1 and 3 suppressed the mRNA expression levels of FAS and ACC. These results demonstrate that inhibition of adipogenesis in 3T3-L1 cells by treatment with 1 and 3 may be mediated in part through down-regulation of the adipogenic transcription factor SREBP-1c and its target genes, such as FAS and ACC.
Lee
IkSoo
I
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Kim
JinPyo
J
Ryoo
InJa
I
Kim
YoungHee
Y
Choo
SooJin
S
Yoo
IckDong
I
Min
ByungSun
B
Na
MinKyun
M
Hattori
Masao
M
Bae
KiHwan
K
D041721:3T3-L1 Cells / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D051379:Mice; D020940:Reishi / Q000737:chemistry*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D051780:Sterol Regulatory Element Binding Protein 1; D014315:Triterpenes