title: The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.
abstract: The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.
authors.affiliation: New York Structural Biology Center, New York, 10027, USA.
authors.forename: James
authors.initials: J
authors.lastname: Love
authors.forename: Filippo
authors.initials: F
authors.lastname: Mancia
authors.forename: Lawrence
authors.initials: L
authors.lastname: Shapiro
authors.forename: Marco
authors.initials: M
authors.lastname: Punta
authors.forename: Burkhard
authors.initials: B
authors.lastname: Rost
authors.forename: Mark
authors.initials: M
authors.lastname: Girvin
authors.forename: Da-Neng
authors.initials: DN
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: John F
authors.initials: JF
authors.lastname: Hunt
authors.forename: Thomas
authors.initials: T
authors.lastname: Szyperski
authors.forename: Eric
authors.initials: E
authors.lastname: Gouaux
authors.forename: Roderick
authors.initials: R
authors.lastname: MacKinnon
authors.forename: Ann
authors.initials: A
authors.lastname: McDermott
authors.forename: Barry
authors.initials: B
authors.lastname: Honig
authors.forename: Masayori
authors.initials: M
authors.lastname: Inouye
authors.forename: Gaetano
authors.initials: G
authors.lastname: Montelione
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Hendrickson
mesh_terms: D002478:Cells, Cultured; D019295:Computational Biology; D018360:Crystallography, X-Ray; D016678:Genome; D023281:Genomics / Q000379:methods*; D008565:Membrane Proteins / Q000235:genetics*; D009518:New York; D017433:Protein Structure, Secondary
chemical_list: D008565:Membrane Proteins