title: Is the abundance of Faecalibacterium prausnitzii relevant to Crohn's disease?
abstract: Reports that bacteria within the Firmicutes phylum, especially the species Faecalibacterium prausnitzii, are less abundant in Crohn's disease (CD) patients and supernatants from cultures of this bacterium are anti-inflammatory prompted the investigation of the possible correlations between the abundance of F. prausnitzii and the response to treatment in patients with gut diseases and healthy controls. In a randomized, double-blind trial, faeces were collected from healthy volunteers, and from patients with active CD, ulcerative colitis (UC) and irritable bowel syndrome before and after treatment. The levels of F. prausnitzii DNA in faecal suspensions were determined by PCR. Treatment by an elemental diet was effective, resulting in decreases in both the Harvey and Bradshaw index (P<0.001) and the concentrations of serum C-reactive protein (P<0.05). The total levels of F. prausnitzii in faecal samples from CD patients at presentation were lower than those in the other groups both before and after the treatment. There was no correlation between F. prausnitzii abundance and the severity of CD before treatment. Clinical improvement unexpectedly correlated with a significant decrease in the abundance of F. prausnitzii, especially the A2-165 subgroup (P<0.05). Our data suggest that a paucity of F. prausnitzii in the gastrointestinal microbial communities is likely to be a minor aetiological factor in CD: recovery following elemental diet is attributed to lower levels of gut flora.
authors.affiliation: School of Biosciences, University of Birmingham, Birmingham, UK.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Jia
authors.forename: Rebekah N
authors.initials: RN
authors.lastname: Whitehead
authors.forename: Lesley
authors.initials: L
authors.lastname: Griffiths
authors.forename: Claire
authors.initials: C
authors.lastname: Dawson
authors.forename: Rosemary H
authors.initials: RH
authors.lastname: Waring
authors.forename: David B
authors.initials: DB
authors.lastname: Ramsden
authors.forename: John O
authors.initials: JO
authors.lastname: Hunter
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Cole
mesh_terms: D003093:Colitis, Ulcerative / Q000382:microbiology; D003424:Crohn Disease / Q000178:diet therapy* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005526:Food, Formulated; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000502:physiology*; D043183:Irritable Bowel Syndrome / Q000382:microbiology; D012720:Severity of Illness Index; D016896:Treatment Outcome