title: Preparation and analysis of microRNA libraries using the Illumina massively parallel sequencing technology.
abstract: MicroRNAs are key regulators of gene expression in diverse biological processes and their importance in embryonic stem cells is indisputable. New 'next-generation' technologies such as Illumina massively parallel sequencing offer vast improvements, in both scale and sensitivity, to microRNA profiling studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.
authors.affiliation: BC Cancer Agency, Genome Sciences Centre, Vancouver, BC, Canada.
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Morin
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Anna-Liisa
authors.initials: AL
authors.lastname: Prabhu
authors.forename: Noreen
authors.initials: N
authors.lastname: Dhalla
authors.forename: Helen
authors.initials: H
authors.lastname: McDonald
authors.forename: Pawan
authors.initials: P
authors.lastname: Pandoh
authors.forename: Angela
authors.initials: A
authors.lastname: Tam
authors.forename: Thomas
authors.initials: T
authors.lastname: Zeng
authors.forename: Martin
authors.initials: M
authors.lastname: Hirst
authors.forename: Marco
authors.initials: M
authors.lastname: Marra
mesh_terms: D053595:Embryonic Stem Cells / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D035683:MicroRNAs