A distinct subset of podoplanin (gp38) expressing F4/80+ macrophages mediate phagocytosis and are induced following zymosan peritonitis.
Macrophages are important tissue resident cells that regulate the dynamics of inflammation. However, they are strikingly heterogeneous. During studies looking at podoplanin (gp38) expression on stromal cells in the murine spleen and peritoneal cavity we unexpectedly discovered that podoplanin was expressed on a subset of F4/80(+) macrophages; a subset which we have termed fibroblastic macrophages (FM). These cells function as phagocytes in vitro as measured by bead mediated phagocytosis assays. FM also exist at high frequency in the peritoneal cavity and in zymosan induced peritonitis in vivo. These FM represent a unique subgroup of F4/80(+) macrophages and their presence in the inflamed peritoneum suggests that they play a role in zymosan induced peritonitis.
Hou
Tie Zheng
TZ
School of Immunity and Infection, MRC Centre for Immune Regulation, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.
Bystrom
Jonas
J
Sherlock
Jonathan P
JP
Qureshi
Omar
O
Parnell
Sonia M
SM
Anderson
Graham
G
Gilroy
Derek W
DW
Buckley
Christopher D
CD
D000818:Animals; D005347:Fibroblasts / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology*; D010587:Phagocytosis; D015054:Zymosan / Q000494:pharmacology; D000098853:Podoplanin
D008562:Membrane Glycoproteins; D015054:Zymosan; C490441:Pdpn protein, mouse; D000098853:Podoplanin