Artificial food colorants inhibit superoxide production in differentiated HL-60 cells.
We tested synthetic food colorants for their antioxidative potential by the in vitro superoxide generation assay in differentiated HL-60 cells in response to phorbol ester. Among the 12 colorants tested, such fluorescein-type red colorants as rose bengal showed potent inhibitory activity without any cytotoxicity under dark conditions. The intracellular accumulation and superoxide anion scavenging effect of rose bengal were at least partly involved in the inhibitory activity.
Qi
Hang
H
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Kawagishi
Machiko
M
Yoshimoto
Motoko
M
Takano
Hiroshi
H
Zhu
Beiwei
B
Shimoishi
Yasuaki
Y
Tada
Mikiro
M
Murata
Yoshiyuki
Y
Nakamura
Yoshimasa
Y
D002454:Cell Differentiation / Q000187:drug effects*; D005505:Food Coloring Agents / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D005505:Food Coloring Agents; D013481:Superoxides