Repair of bone defects around dental implants with bone morphogenetic protein/fibroblast growth factor-loaded porous calcium phosphate cement: a pilot study in a canine model.
OBJECTIVE
To study the effects of the bone morphogenetic protein-2 (BMP-2)/fibroblast growth factor (bFGF)-loaded porous calcium phosphate cement (CPC) on the repair of bone defects around dental implants.


METHODS
Thirty critical-sized bone defects in beagle dogs were repaired with engineered bone composed of autologous bone-marrow-derived mesenchymal stem cells (BMSCs), BMP-2, bFGF and CPC. Repairs were divided into six groups: BMSC/BMP-2/bFGF/CPC, BMSC/BMP-2/CPC, BMSC/bFGF/CPC, BMSC/CPC, CPC and no treatment. Polychrome sequential fluorescent labels were also performed post-operatively. Fluorescence histological examinations of undecalcified sections at post-operative week 12 were performed to clarify changes in the new bone around the dental implants.


RESULTS
The animals exhibited a perfect post-operative course, with none experiencing any infection. Undecalcified sections showed that new bone was actively formed in the BMP-2/bFGF group after 12 weeks. The bone mineralization apposition rate was better in the BMP-2/bFGF group than in the other groups (P<0.05).


CONCLUSIONS
BMP-2 and bFGF together are more effective than either one alone in promoting the formation of new bone and may exert a synergistic activity at bone defects around dental implants.
Wang
Lei
L
Department of Stomatology, East Hospital affiliated to Tongji University, Jimo Road No. 150, Shanghai, China.
Zou
Duohong
D
Zhang
Shijie
S
Zhao
Jun
J
Pan
Kefeng
K
Huang
Yuanliang
Y
D000704:Analysis of Variance; D000818:Animals; D001843:Bone Cements / Q000494:pharmacology*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D015921:Dental Implants; D004285:Dogs; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D008334:Mandible / Q000187:drug effects* / Q000601:surgery*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000187:drug effects*; D010865:Pilot Projects; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*
D001843:Bone Cements; D019485:Bone Morphogenetic Proteins; D002130:Calcium Phosphates; D015921:Dental Implants; D005346:Fibroblast Growth Factors