Toxicological insult of canalicular function in rat hepatocyte couplets: The role of calcium.
The biliary canalicular function of rat hepatocyte couplets was more sensitive to an increase in cytosolic calcium induced by A23187 (up to 30 mum) than by thapsigargin (up to 50 nm). This suggests that canalicular function was not disrupted by the release of endoplasmic reticulum calcium stores, although this was seen to stimulate canalicular contraction. Oxidative stress induced by menadione apparently disrupted canalicular function at non-lethal concentrations (up to 100 mum) by way of a mechanism other than the involvement of calcium.
Stone
V
V
School of Biochemistry, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Lankester
D J
DJ
Wilton
J C
JC
Coleman
R
R
Chipman
J K
JK