title: Splenic function and red blood cell deformability: The beneficial effects of spleen autotransplantation in animal experiments.
abstract: Erythrocyte deformability could be a suitable parameter reflecting hyposplenic-asplenic conditions after splenectomy or spleen autotransplantation. The aim of this study was to analyze the efficacy of spleen autotransplantation using filtrometry and ektacytometry for testing erythrocyte deformability, besides complex structural, functional, hematological, hemostaseological and immunological examinations. Healthy beagle dogs were subjected to the study in the following experimental groups: "Sham"-sham operated control, "SE"-splenectomy, "AU-5" and "AU-10"-autotransplantation with 5 or 10 spleen chips using "Furka's spleen chip" technique after splenectomy. Blood samples were taken before the operations and monthly afterwards for 1 year and bi-monthly till the end of the 2nd postoperative year. Erythrocyte deformability was measured by bulk filtrometry (relative cell transit time, RCTT) and on the 20th-24th postoperative months by ektacytometry (elongation index, EI), too. RCTT was elevated in operated groups till the 12th month. On the 20th/24th months "SE"-group expressed the highest level, while the "AU"-groups were close to "Sham"-group. Using ektacytometer we found that on the 20th/24th month both EImax and SS1/2 showed the lowest values (because of unusual curve characteristic) in "SE"-group and "AU"-groups almost reached the "SH"-group. Spleen autotransplantation partially restored the normal rheological conditions. Both filtrometry and ektacytometry was suitable to investigate hyposplenic-asplenic conditions.
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. imiko@med.unideb.hu
authors.forename: Iren
authors.initials: I
authors.lastname: Miko
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.forename: Erika
authors.initials: E
authors.lastname: Sajtos
authors.forename: Endre
authors.initials: E
authors.lastname: Brath
authors.forename: Katalin
authors.initials: K
authors.lastname: Peto
authors.forename: Andrea
authors.initials: A
authors.lastname: Furka
authors.forename: Gyorgyi
authors.initials: G
authors.lastname: Szabo
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kiss
authors.forename: Sandor
authors.initials: S
authors.lastname: Imre
authors.forename: Istvan
authors.initials: I
authors.lastname: Furka
mesh_terms: D000818:Animals; D004285:Dogs; D004907:Erythrocyte Deformability; D005500:Follow-Up Studies; D018056:Hemorheology; D013154:Spleen / Q000502:physiology* / Q000637:transplantation*; D013156:Splenectomy