Metabolism of alternariol monomethyl ether by porcine liver and intestinal mucosa in vitro.
The in vitro metabolism of alternariol monomethyl ether (AME) was investigated in intestinal mucosa and liver samples from gilts. Homogenized tissue samples were incubated with AME in the presence of NADPH, UDPGA or glutathione. The results indicate that conjugation with glucuronic acid is the predominant metabolic route of AME in the gilt. This occurs to a greater extent in the intestinal mucosa than in the liver (65.7 +/- 25.3 and 5.0 +/- 3.3 nmol AME conjugated/mg protein/hr, respectively). Demethylation of AME to alternariol (AOH) was a minor metabolic route, occurring only in liver in the presence of NADPH (0.31 +/- 0.26 nmol AOH formed/mg protein/hr). No glutathione conjugation of AME was found in this investigation.
Olsen
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Istituto Tossine e Micotossine de Parassiti Vegetali, Consiglio Nazionale delle Richerche, Via G. Amendola 197/F, I-70126 Bari, Italy; Department of Animal Nutrition and Management, P.O. Box 7046, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden.
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