[The interventional effect of anti-basic fibroblast growth factor on the model of bleomycin-induced pulmonary fibrosis in rats].
OBJECTIVE
To study the effect of anti-basic jibroblast grouth factor (bFGF) on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.


METHODS
Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin (model group, group M). Then the rats received anti-bFGF on 1, 2, 3, 8, 12, 19, 25 days intracavitary injection (group K), normal saline(group C) orally. Normal controls received normal saline both intratracheally and orally. Five rats in each group were sacrificed on 1.4 week after intratracheal instillation. Histological changes of the lungs were evaluated by HE stain and Massons trichrome stain. Lung expression of bFGF proteins was assessed by immunohistochemistry and the level of bFGF protein in serum and BALF was further measured by ELISA.


RESULTS
Pulmonary fibrosis of group M was higher than that of group C. bFGF in group M was higher than that in group C in lung, serum and BALF on 1.4 week. Pulmonary fibrosis of group K was lower than that of group M. bFGF in group K was lower than that in group M in lung, serum and BALF on 1.4 week.


CONCLUSIONS
Anti-bFGF alleviates bleomycin-induced pulmonary fibrosis in rats. Inhibiting the expressions of bFGF in lung tissues may be one of the mechanisms.
Mu
Jian-qin
JQ
Affiliated Hospital of Medical College of Chinese People Armed Police Force, Tianjin 300162, China.
Wei
Lu-qing
LQ
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000494:pharmacology*; D001761:Bleomycin; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000276:immunology*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D016222:Fibroblast Growth Factor 2; D001761:Bleomycin