Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano.
We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.
Horikawa
Kazuki
K
Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
Yamada
Yoshiyuki
Y
Matsuda
Tomoki
T
Kobayashi
Kentarou
K
Hashimoto
Mitsuhiro
M
Matsu-ura
Toru
T
Miyawaki
Atsushi
A
Michikawa
Takayuki
T
Mikoshiba
Katsuhiko
K
Nagai
Takeharu
T
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D004023:Dictyostelium; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D007202:Indicators and Reagents / Q000032:analysis / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D015398:Signal Transduction; D015027:Zebrafish
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D002118:Calcium