title: Arsenic resistance operon structure in Leptospirillum ferriphilum and proteomic response to arsenic stress.
abstract: The response of Leptospirillum ferriphilum ML-04 to arsenic stress was analyzed using two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Thirty-eight of 65 significantly differentially expressed arsenic response proteins were identified, and 25 of them have known functions. Three proteins are arsenic resistance system (ARS) member proteins. Two ars operons appear to be present in this strain. In addition to the ARS system, phosphate regulation and glutathione (GSH) synthesis appear involved in As[V] and As[III] tolerance, respectively. These findings provide information potentially useful for the genetic engineering of arsenic resistant organisms.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Jianqun
authors.initials: J
authors.lastname: Lin
authors.forename: Shuang
authors.initials: S
authors.lastname: Mi
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lin
mesh_terms: D001151:Arsenic / Q000633:toxicity*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005798:Genes, Bacterial / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001151:Arsenic