Suppression of migration and invasion of PC3 prostate cancer cell line via activating E-cadherin expression by small activating RNA.
ABSTRACT Small activating RNAs are a recently discovered group of small, noncoding, and double-stranded RNA molecules that can induce sequence-specific transcriptional gene activation by targeting gene promoter regions. In the present study, we demonstrate that induction of E-cadherin expression by small activating RNA leads to suppression of migration and invasion of PC3 prostate cancer cells. The inhibitory effect was associated with relocalization of β -catenin from the nucleus to the plasma membrane and decreased β -catenin-mediated transactivation. These data suggest that activation of E-cadherin by small activating RNA may have a therapeutic benefit for prostate and other types of cancer.
Mao
Qiqi
Q
Department of Urology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
Zheng
Xiangyi
X
Yang
Kai
K
Qin
Jie
J
Bai
Yu
Y
Jia
Xiaolong
X
Li
Yubing
Y
Xie
Liping
L
D015153:Blotting, Western; D015820:Cadherins / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012330:RNA, Double-Stranded / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism
D015820:Cadherins; D012330:RNA, Double-Stranded; D051176:beta Catenin