[Characteristic of one-paired pea virus].
The new virus isolated from Vicia unijuga A.Br. with filament particles with size 1000-1200 x 10-12 nm is revealed. A thermal inactivation point is 55 degrees C; dilution end point - 10(-5)-10(-6) longevity in vitro in broad bean sap--less than one day. It is transferred by aphids and by pea, bean and broad bean seeds. The plants of Fabaceae, Solanaceae and Chenopodiaceae fam. were affected by this virus isolate. The virus yield was 40-50 mg per 100 g of leaves. The ratio of absorption E260/E280 corresponded to 1.4-1.5. The molecular mass of a core protein of the virus was 34 kD. The virus has a high immunogenic properties--titer is 1:256000 (indirect method of ELISA). It is presumably identified as a member of Closteroviridae.
Kakareka
N N
NN
Kozlovskaia
Z N
ZN
Volkov
Iu G
IuG
D036022:Capsid Proteins / Q000737:chemistry; D029381:Closteroviridae / Q000737:chemistry / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D031306:Vicia / Q000821:virology*; D014771:Virion / Q000737:chemistry / Q000302:isolation & purification
D036022:Capsid Proteins