In situ encapsulation of laccase in microfibers by emulsion electrospinning: preparation, characterization, and application.
Laccase from Trametes versicolor was successfully in situ encapsulated into the poly(D,L-lactide) (PDLLA)/PEO-PPO-PEO (F108) electrospun microfibers by emulsion electrospinning. The porous morphology of electrospun microfibers was observed with scanning electron microscope, and the core-shell structure of microfibers and existence of laccase in microfibers were proved by laser confocal scanning microscopy micrograph. In this study, fibrous porosity and core-shell structure are advantageous to the activity and stability preservation of immobilized laccase. The activity of immobilized laccase could retain over 67% of that of the free enzyme. After 10 successive runs in the enzyme reactor, the immobilized laccase could also maintain 50% of its initial activity. Crystal violet dye was successfully degraded by the PDLLA/F108-laccase electrospun microfiber membranes. It was observed that the immobilized laccase possessed a broadening pH range of catalysis activity compared to free laccase.
Dai
Yunrong
Y
State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
Niu
Junfeng
J
Liu
Jia
J
Yin
Lifeng
L
Xu
Jiangjie
J
D052160:Benzothiazoles / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D004655:Emulsions; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005840:Gentian Violet / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D042845:Laccase / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000494:pharmacology; D013451:Sulfonic Acids / Q000494:pharmacology; D055454:Trametes / Q000201:enzymology*
D052160:Benzothiazoles; D004396:Coloring Agents; D004655:Emulsions; D004800:Enzymes, Immobilized; D011091:Polyesters; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C033616:poly(lactide); D042845:Laccase; D005840:Gentian Violet