title: Roles of the respiratory syncytial virus trailer region: effects of mutations on genome production and stress granule formation.
abstract: The 5' extragenic trailer region of respiratory syncytial virus (RSV) is known to be necessary for genome replication, but is more than three times the length of the 3' leader replication promoter, raising the possibility that trailer might play an additional role in viral replication. To examine this, mutant recombinant viruses were constructed in which the trailer region was truncated or substituted with leader-complement sequence. This analysis showed that the complete trailer increased promoter activity, facilitating genome production and viral multiplication. In addition, trailer-containing viruses did not induce stress granules, whereas the leader-complement virus mutant did, resulting in poor multi-cycle viral growth. These data demonstrate that although the RSV trailer does not contain a unique essential sequence, it augments virus growth by enabling optimal genome production. In addition, a sequence at the 5' terminal end of the trailer region allows RSV to subvert stress granule formation.
authors.affiliation: Department of Microbiology, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Hanley
authors.forename: David R
authors.initials: DR
authors.lastname: McGivern
authors.forename: Michael N
authors.initials: MN
authors.lastname: Teng
authors.forename: Robin
authors.initials: R
authors.lastname: Djang
authors.forename: Peter L
authors.initials: PL
authors.lastname: Collins
authors.forename: Rachel
authors.initials: R
authors.lastname: Fearns
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000821:virology; D005260:Female; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D012367:RNA, Viral