Lack of AFB(1)-induced mutagenicity in the in vitro hepatocyte micronucleus assay: Investigation of possible mechanisms.
Several mechanistic experiments were carried out to investigate negative results obtained with aflatoxin B(1) (AFB(1)) in the in vitro hepatocyte micronucleus assay. The data showed that the lack of AFB(1)-induced mutogenicity was not due to deficient metabolic activation of the mycotoxin or to an inefficiency of the test protocol. It appeared that the cytotoxic actions of AFB(1), specifically the interference with hepatocyte proliferation, prevented the formation of micronuclei.
Müller
K
K
Institute for Drugs, Federal Health Office, Seestrasse 10, 13353 Berlin, Germany.
Kasper
P
P
Müller
L
L