Effects of lead and mercury on the blood proteome of children.
Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.
Birdsall
Robert E
RE
Department of Chemistry, SUNY Oswego, Oswego, New York 13126, USA.
Kiley
Michael P
MP
Segu
Zaneer M
ZM
Palmer
Christopher D
CD
Madera
Milan
M
Gump
Brooks B
BB
MacKenzie
James A
JA
Parsons
Patrick J
PJ
Mechref
Yehia
Y
Novotny
Milos V
MV
Bendinskas
Kestutis G
KG
D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000097:blood / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D002853:Chromatography, Liquid; D003430:Cross-Sectional Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007855:Lead Poisoning / Q000097:blood*; D008297:Male; D008630:Mercury Poisoning / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry; D020543:Proteome / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012737:Sex Factors; D012959:Socioeconomic Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000097:blood / Q000378:metabolism
D001057:Apolipoproteins E; D001798:Blood Proteins; D010446:Peptide Fragments; D020543:Proteome; D014357:Trypsin