title: Maintenance of red blood cell integrity by AMP-activated protein kinase alpha1 catalytic subunit.
abstract: AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. We previously showed that AMPKalpha1-/- mice develop moderate anemia associated with splenomegaly and high reticulocytosis. Here, we report that splenectomy of AMPKalpha1-/- mice worsened anemia supporting evidence that AMPKalpha1-/- mice developed a compensatory response through extramedullary erythropoiesis in the spleen. Transplantation of bone marrow from AMPKalpha1-/- mice into wild-type recipients recapitulated the hematologic phenotype. Further, AMPKalpha1-/- red blood cells (RBC) showed less deformability in response to shear stress limiting their membrane flexibility. Thus, our results highlight the crucial role of AMPK to preserve RBC integrity.
authors.affiliation: Institut Cochin, Université Paris Descartes, Centre National de la Recherche Scientifique (UMR8104), Paris, France; INSERM, U1016, Paris, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Foretz
authors.forename: Soizic
authors.initials: S
authors.lastname: Guihard
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Leclerc
authors.forename: Véronique
authors.initials: V
authors.lastname: Fauveau
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Couty
authors.forename: Fabienne
authors.initials: F
authors.lastname: Andris
authors.forename: Murielle
authors.initials: M
authors.lastname: Gaudry
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Andreelli
authors.forename: Sophie
authors.initials: S
authors.lastname: Vaulont
authors.forename: Benoit
authors.initials: B
authors.lastname: Viollet
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000097:blood* / Q000172:deficiency / Q000235:genetics; D000740:Anemia / Q000097:blood / Q000201:enzymology / Q000235:genetics; D000818:Animals; D016026:Bone Marrow Transplantation; D004907:Erythrocyte Deformability / Q000235:genetics / Q000502:physiology*; D004920:Erythropoiesis; D006411:Hematopoiesis, Extramedullary; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009996:Osmotic Fragility; D013156:Splenectomy; D013163:Splenomegaly / Q000097:blood / Q000201:enzymology / Q000235:genetics
chemical_list: C548179:Prkaa1 protein, mouse; D055372:AMP-Activated Protein Kinases