Vacuolar amino acid transporter Avt5p is responsible for lithium uptake in Schizosaccharomyces pombe.
The fission yeast Schizosaccharomyces pombe was sensitive to salinity; cell growth was stopped by 0.5 M NaCl and by 10 mM LiCl. The avt5+ gene encodes a vacuolar transporter with a broad specificity for amino acids. We found that the avt5Delta mutant became highly tolerant of Li+ and Na+ in growth. Concanamycin A-sensitive Li+ uptake as well as cellular Li+ content was lower in the avt5 mutant, suggesting a role of Avt5p in cellular uptake of toxic Li+.
Iwaki
Tomoko
T
Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University, Ehime, Japan.
Sekito
Takayuki
T
Kakinuma
Yoshimi
Y
D001692:Biological Transport; D002451:Cell Compartmentation; D042541:Intracellular Space / Q000378:metabolism; D008094:Lithium / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
C550143:Avt5 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D033921:Vesicular Transport Proteins; D008094:Lithium