title: Oxidative DNA damage contributes to the toxic activity of propylparaben in mammalian cells.
abstract: Propyl p-hydroxybenzoate, commonly referred to as propylparaben, is the most frequently used preservative to inhibit microbial growth and extend shelf life of a range of consumer products. The objective of this study was to provide further insight into the toxicological profile of this compound, because of the current discrepancy in the literature with regard to the safety of parabens. The Vero cell line, derived from the kidney of the green monkey, was selected to evaluate the adverse effects of propylparaben by use of a set of mechanistically relevant endpoints for detecting cytotoxicity and genotoxic activities. Our results demonstrate that exposure to the compound for 24h causes changes in cell-proliferation rates rather than in cell viability. A significant and dose-dependent decline in the percentage of mitotic cells was observed at the lowest concentration tested, mainly due to cell-cycle arrest at the G0/G1 phase. Immunodetection techniques revealed that induction of DNA double-strand breaks and oxidative damage underlies the cytostatic effect observed in treated Vero cells. Additional studies are in progress to extend these findings, which define a novel mode of action of propylparaben in cultured mammalian cells.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Pérez Martín
authors.forename: Ana
authors.initials: A
authors.lastname: Peropadre
authors.forename: Oscar
authors.initials: O
authors.lastname: Herrero
authors.forename: Paloma
authors.initials: P
authors.lastname: Fernández Freire
authors.forename: Verónica
authors.initials: V
authors.lastname: Labrador
authors.forename: María José
authors.initials: MJ
authors.lastname: Hazen
mesh_terms: D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005520:Food Preservatives / Q000633:toxicity; D008940:Mitotic Index; D018384:Oxidative Stress / Q000235:genetics*; D010226:Parabens / Q000633:toxicity*; D011310:Preservatives, Pharmaceutical / Q000633:toxicity; D014709:Vero Cells
chemical_list: D005520:Food Preservatives; D010226:Parabens; D011310:Preservatives, Pharmaceutical; C006068:propylparaben