title: Screen-printed poly(3,4-ethylenedioxythiophene) (PEDOT): A new electrochemical mediator for acetylcholinesterase-based biosensors.
abstract: This work describes the use of a PEDOT:PSS-based conductive polymer for designing AChE-based biosensors. The transducers were obtained directly by screen-printing a PEDOT:PSS suspension on the surface of thick film carbon electrodes. The obtained working electrodes showed a high conductivity when compared with electrodes modified with conventional mediators like cobalt phthalocyanine or tetracyanoquinodimethane. The PEDOT:PSS polymer was shown to be suitable for thiocholine oxidation, allowing the measurement of AChE activity at 100 mV vs Ag/AgCl. The high conductivity of PEDOT:PSS allowed the accurate detection of the organophosphate insecticide chlorpyrifos-oxon at concentrations as low as 4x10(-9)M, corresponding to an inhibition ratio of 5%.
authors.affiliation: Université de Perpignan, Via Domitia, IMAGES, Bat. S, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Istamboulie
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sikora
authors.forename: Elena
authors.initials: E
authors.lastname: Jubete
authors.forename: Estibalitz
authors.initials: E
authors.lastname: Ochoteco
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
mesh_terms: D000110:Acetylcholinesterase; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004390:Chlorpyrifos / Q000032:analysis; D004553:Electric Conductivity; D004566:Electrodes; D007306:Insecticides / Q000032:analysis; D010755:Organophosphates / Q000032:analysis*; D011108:Polymers; D013860:Thiocholine / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D007306:Insecticides; D010755:Organophosphates; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D013860:Thiocholine; D000110:Acetylcholinesterase; D004390:Chlorpyrifos