Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2.
Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 A resolution and belonged to space group P2, with unit-cell parameters a=70.2, b=52.2, c=153.9 A, beta=102.8 degrees.
Cheng
Xia
X
Key Laboratory of Environment Veterinary Science, Shanxi Agricultural University, Shanxi, People's Republic of China.
Lu
Guangwen
G
Qi
Jianxun
J
Cheng
Hao
H
Gao
Feng
F
Wang
Jundong
J
Yan
Jinghua
J
D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017011:Streptococcus suis / Q000201:enzymology*
D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase