title: Development and characterization of mouse monoclonal antibodies specific for chicken interleukin 18.
abstract: Four mouse monoclonal antibodies (mAbs) which are specific for chicken interleukin 18 (chIL18) were produced and characterized by enzyme-linked immunosorbent assay (ELISA), Western blotting, quantitative real-time PCR and neutralization assays. Using Western blot analysis, monoclonal antibodies specific for chIL18 identified a 23 kDa Pichia pastoris-expressed chIL18 and 66 kDa E. coli-derived MBP fusion protein of chIL18. Bioassays for chIL18 using primary chicken spleen cells showed dose-dependent IFN-γ mRNA expression and induction of IFN-γ from primary splenocytes, and triggered nitric oxide (NO) production in the HD11 macrophage cell line. These mAbs showed neutralizing chIL18 activity. Taken together, these mouse mAbs which detect chicken IL-18 will be significant new immune reagents and useful tools for basic and applied research in poultry.
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-759, Republic of Korea.
authors.forename: Yeong Ho
authors.initials: YH
authors.lastname: Hong
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Sung Hyen
authors.initials: SH
authors.lastname: Lee
authors.forename: Myeong-Sun
authors.initials: MS
authors.lastname: Park
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
authors.forename: Joanna
authors.initials: J
authors.lastname: Labresh
authors.forename: Dannielle
authors.initials: D
authors.lastname: Tompkins
authors.forename: Cynthia
authors.initials: C
authors.lastname: Baldwin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D057134:Antibodies, Neutralizing / Q000096:biosynthesis; D000918:Antibody Specificity; D030161:Avian Proteins / Q000276:immunology*; D015153:Blotting, Western; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D066298:In Vitro Techniques; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D030161:Avian Proteins; D020382:Interleukin-18; D011993:Recombinant Fusion Proteins