title: Effect of ozone on response to ovalbumin & its modulation by vitamins C & E in sensitized guinea pigs.
abstract: OBJECTIVE
  Exposure to ozone and asthma are both associated with increased oxidative stress. Exposure to ozone therefore, may potentiate the airway response to allergens. We undertook this study to investigate the effect of ozone exposure on airway response to ovalbumin in sensitized guinea pigs and its modulation by dietary supplementation with antioxidant vitamins C and E.
  
  
  METHODS
  After in vivo measurements of specific airways conductance (SGaw) and airway hyperresponsiveness (AHR) to inhaled histamine, guinea pigs were sensitized to ovalbumin and divided into three groups: (i) sensitized; (ii) sensitized and exposed daily to ozone; and (iii) sensitized, exposed daily to ozone and given dietary supplementation with vitamin C, 2 mg/kg body wt and E, 7 IU/kg body wt. A control group of nonsensitized animals was included. After 4 wk, AHR was measured again and animals were challenged with inhaled ovalbumin. Changes in SGaw were followed for early and late airway bronchoconstrictive responses. The following measurements were obtained: (i) parameters of oxidative stress--plasma malonaldehyde (MDA) as marker of lipid peroxidation and superoxide anion generation by leukocytes and bronchoalveolar lavage (BAL) cells; (ii) antioxidant status: red cell superoxide dismutase (SOD); and (iii) glutathione peroxidase (GPx). BAL cytology was studied.
  
  
  RESULTS
  Ozone exposure resulted in an increase in AHR and early and late bronchoconstrictive responses after ovalbumin challenge, greater superoxide anion generation in BAL cells, higher plasma MDA levels and decrease in red cell SOD activity. Dietary supplementation with vitamin C and E prevented or ameliorated these responses.
  
  
  CONCLUSIONS
  Exposure to ozone at concentrations of 0.12 ppm for 2 h daily for 4 wk enhances the airway response to allergens in sensitized guinea pigs. Dietary supplementation with antioxidant vitamins E and C, affords variable degree of protection against this enhancement.
authors.affiliation: Department of Cardiorespiratory Physiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India. skchhabra@mailcity.com
authors.forename: S K
authors.initials: SK
authors.lastname: Chhabra
authors.forename: Abdul
authors.initials: A
authors.lastname: Yasir
authors.forename: K
authors.initials: K
authors.lastname: Chaudhry
authors.forename: B
authors.initials: B
authors.lastname: Shah
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016022:Case-Control Studies; D006168:Guinea Pigs; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010047:Ovalbumin / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000378:metabolism* / Q000517:prevention & control; D018709:Statistics, Nonparametric; D013481:Superoxides / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000393:Air Pollutants; D013481:Superoxides; D014810:Vitamin E; D010126:Ozone; D010047:Ovalbumin; D001205:Ascorbic Acid