Degradability of poly(L-lysine) and poly(DL-aminoserinate) complexed with a polyanion under conditions modelling physico-chemical characteristics of body fluids.
Poly(L-lysine), (PLL), and poly(DL-amino serinate), (PSA), are respectively enzymatically and hydrolytically degradable polycations. This work was aimed at investigating their degradability when they are complexed with polyanions, namely poly(acrylic acid) and poly(L-lysine citramide), taken as simple models of DNA in polyplexes. Comparison was made with degradation characteristics of the same polycations in solution in the absence of polyanion on the basis of size exclusion chromatography and capillary zone electrophoresis. Complexed PLL remained enzymatically degradable by trypsin, an endopeptidase, but was no longer degradable by aminopeptidase, an exopeptidase. Trypsin yielded a mixture of trilysin and tetralysin. Complexed PSA remained hydrolytically degradable in aqueous media. The hydrolysis of PSA led to DL-serine. However, traces of anionic species were also detected that were identified as residues of constituting repeating units issued from the N-benzyloxycarbonyl polyaminoserinate precursor (PSAZ).
Leclercq
Laurent
L
Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Université de Montpellier 1, Equipe CRBA, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34093 Montpellier Cedex 5, France. leclercq@univ-montp1.fr
Boustta
Mahfoud
M
Rixte
Johan
J
Vert
Michel
M
D000180:Acrylic Resins / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D055598:Chemical Phenomena; D010450:Endopeptidases / Q000737:chemistry; D008239:Lysine / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D000071228:Polyelectrolytes; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry
D000180:Acrylic Resins; D010450:Endopeptidases; D008239:Lysine; D000071228:Polyelectrolytes; D011107:Polylysine; D011108:Polymers; D012965:Sodium Chloride; C510489:poly(lysine citramide); C006903:carbopol 940