Methods to measure nuclear export of beta-catenin using fixed and live cell assays.
beta-Catenin is a multifunctional protein which is overexpressed in several types of cancers. The subcellular location of beta-catenin at the membrane junctions or in the nucleus determines its function in cell adhesion and transcription activation, respectively. The aberrant localization of beta-catenin in the nucleus activates transcription of a number of genes involved in cancer progression. Our laboratory studies intracellular trafficking of beta-catenin with particular emphasis on its movement and regulation in and out of the nucleus. In this chapter, we outline a number of assays to measure the nuclear export of beta-catenin in fixed and live cells.
Sharma
Manisha
M
Westmead Institute for Cancer Research, Westmead Millennium Institute at Westmead Hospital, The University of Sydney, Westmead, NSW, Australia.
Henderson
Beric R
BR
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002470:Cell Survival; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010539:Permeability; D014162:Transfection; D051176:beta Catenin / Q000737:chemistry / Q000378:metabolism*
D051176:beta Catenin