Propofol and aminophylline antagonize each other during the mobilization of intracellular calcium in human umbilical vein endothelial cells.
This study examined whether propofol and aminophylline affect the mobilization of intracellular calcium in human umbilical vein endothelial cells. Intracellular calcium was measured using laser scanning confocal microscopy. Cultured and serum-starved cells on round coverslips were incubated with propofol or aminophylline for 30 min, and then stimulated with lysophosphatidic acid, propofol and aminophylline. The results were expressed as relative fluorescence intensity and fold stimulation. Propofol decreased the concentration of intracellular calcium, whereas aminophylline caused increased mobilization of intracellular calcium in a concentration-dependent manner. Propofol suppressed the lysophosphatidic acid-induced mobilization of intracellular calcium in a concentration-dependent manner. Propofol further prevented the aminophylline-induced increase of intracellular calcium at clinically relevant concentrations. However, aminophylline reversed the inhibitory effect of propofol on the elevation of intracellular calcium by lysophosphatidic acid. Our results suggest that propofol and aminophylline antagonize each other on the mobilization of intracellular calcium in human umbilical vein endothelial cells at clinically relevant concentrations. Serious consideration should be given to how this interaction affects mobilization of intracellular calcium when these two drugs are used together.
Son
Hee-Jeong
HJ
Department of Anesthesiology and Pain Medicine, Kangwon National University Medical School, Chuncheon, Korea.
Lim
Young-Cheol
YC
Ha
Kwon-Soo
KS
Kang
Seong-Sik
SS
Cheong
Il-Young
IY
Lee
Sang-Jin
SJ
Park
Seung-Woo
SW
Hwang
Byeong-Moon
BM
D000628:Aminophylline / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D018686:Anesthetics, Intravenous / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D001993:Bronchodilator Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology; D018613:Microscopy, Confocal; D015742:Propofol / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology
D018686:Anesthetics, Intravenous; D001993:Bronchodilator Agents; D008246:Lysophospholipids; D000628:Aminophylline; C032881:lysophosphatidic acid; D002118:Calcium; D015742:Propofol