Altered signaling through IL-12 receptor in children with very high serum IgE levels.
An alteration of Th1/Th2 homeostasis may lead to diseases in humans. In this study, we investigated whether an impaired IL-12R signaling occurred in children with elevated serum IgE levels divided on the basis of the IgE levels (group A: >2000kU/l; group B: <2000kU/l). We evaluated the integrity of the IL-12R signaling through the analysis of phosphorylation/activation of STAT4, and mRNA expression and membrane assembly of the receptor chains. At a functional level, a proliferative defect of lymphocytes from group A patients was observed. In these patients, an abnormal IL-12R signaling was documented, and this finding was associated with abnormal expression of the IL-12Rbeta2 chain. Our data indicate that in patients with very high IgE levels the generation of Th1 response is impaired, and that this abnormality associates with abnormal IL-12R signaling.
Fusco
Anna
A
Department of Pediatrics, "Federico II" University, Naples, Italy.
Vigliano
Ilaria
I
Palamaro
Loredana
L
Cirillo
Emilia
E
Aloj
Giuseppina
G
Piscopo
Giovanna
G
Giardino
Giuliana
G
Pignata
Claudio
C
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000378:metabolism; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D010766:Phosphorylation; D053707:Receptors, Interleukin-12 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D050797:STAT4 Transcription Factor / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D018417:Th1 Cells / Q000276:immunology*
D053707:Receptors, Interleukin-12; D050797:STAT4 Transcription Factor; C494090:STAT4 protein, human; D007073:Immunoglobulin E