Performing enzyme-free H2O2 biosensor and simultaneous determination for AA, DA, and UA by MWCNT-PEDOT film.
An enzyme-free hydrogen peroxide (H2O2) biosensor based on MWCNT-PEDOT film modified electrode has been successfully performed on glassy carbon electrode (GCE) and indium tin oxide (ITO) electrode. At an applied potential of -0.5V vs. Ag/AgCl, the MWCNT-PEDOT electrode exhibited linearly dependence on H2O2 concentration in the range of 0.1-9.8 mM. It can be observed two significantly linear sections. One shows sensitivity of 943 μM mM(-1) cm(-2) with signal/noise of 6; and the other one shows sensitivity of 174 μM mM(-1) cm(-2) with signal/noise of 4 in pH 7 PBS. It also presented excellent stability at room temperature, with a variation of response current less than 5% over 30 days. Moreover, the sensor was characterized by cyclic voltammetry (CV), scanning electronic microscopy (SEM), atomic force microscopy (AFM), and different pulse voltammetry (DPV). This sensor also can simultaneously detect AA, DA, and UA and can be utilized to develop multifunctional biosensors.
Lin
Kuo-Chiang
KC
Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC.
Tsai
Tsung-Hsuan
TH
Chen
Shen-Ming
SM
D015374:Biosensing Techniques / Q000295:instrumentation*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004798:Enzymes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D004798:Enzymes; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D006861:Hydrogen Peroxide