Unilateral renal artery stenosis causes a chronic vascular inflammatory response in ApoE-/- mice.
Unilateral renal artery ligation (RAL) causes a short-term vascular inflammatory reaction in ApoE-/- mice, but whether this persists is unknown. Partial constriction of the right renal artery resulted in a mild, sustained increase in systolic blood pressure over the 90 days of the study, whereas there was no change in blood pressure in mice that underwent sham-surgery. Atheroma was present in the abdominal aorta, with pronounced staining for macrophages (39% +/- 9% of area of atheroma) and monocyte chemoattractant-1 (MCP-1; 40%+/-15%) in mice with unilateral RAL. Atheroma was also present in the carotid arteries, with pronounced staining for macrophages (56%+/-15%) and MCP-1 (53%+/-21%). Non-muscle myosin-A, a marker of smooth muscle cell dedifferentiation, was observed in atheroma within the abdominal aorta and carotid arteries in mice that underwent unilateral RAL. There were no atheroma and minimal staining for macrophages, MCP-1 or non-muscle myosin A in carotid arteries or abdominal aorta of mice that underwent sham surgery.


CONCLUSIONS
Chronic unilateral partial RAL results in the formation of atheroma in the aorta and carotid arteries of ApoE-/- mice that is characterized by chronic inflammation and dedifferentiation of smooth muscle cells.
Stouffer
G A
GA
Division of Cardiology, University of North Carolina, Chapel Hill, NC 27599-7075, USA. rstouff@med.unc.edu
Pathak
A
A
Rojas
M
M
D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology; D018932:Chemokine CCL2 / Q000378:metabolism; D004195:Disease Models, Animal; D006978:Hypertension, Renovascular / Q000209:etiology / Q000503:physiopathology; D007249:Inflammation / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D008026:Ligation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D018995:Myosin Heavy Chains / Q000378:metabolism; D058226:Plaque, Atherosclerotic / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D012078:Renal Artery Obstruction / Q000150:complications*; D014657:Vasculitis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology
D001057:Apolipoproteins E; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D018995:Myosin Heavy Chains