title: [Biodiversity of phosphate-dissolving and plant growth--promoting endophytic bacteria of two crops].
abstract: OBJECTIVE
  We isolated and characterized phosphate-dissolving endophytic bacteria from two commonly cultivated crops.
  
  
  METHODS
  Phosphate-dissolving endophytic bacteria were isolated by plating and screening from interior tissues of rape and maize plants on NBRIP medium with tricalcium phosphate as sole phosphate source. Bacteria were characterized regarding characteristics that may be relevant for a beneficial plant-microbe interaction-indoleacetic acid, siderophore and 1-aminocyclopropane-1-carboxylic acid deaminase production,and further classified by restriction analysis of 16S rDNA. Eleven typical strains were identified by 16S rDNA sequence analysis.
  
  
  RESULTS
  Thirty-two phosphate-dissolving endophytic bacteria were isolated from maize and rape plants and classified by restriction analysis of 16S rDNA in 8 different taxonomic groups at the similarity level of 76%. All the isolates could release phosphate from tricalcium phosphate and decrease the pH of the medium. The maximum phosphate content (537.6 mg/L) in the solution was obtained with strain M1L5. Thirteen isolates isolated from rape produced indoleacetic acid and siderophore, 68.4% and 63.2% of the strains isolated from maize produced indoleacetic acid and siderophore,respectively. 63.2% of the strains isolated from maize were able to grow on 1-aminocyclopropane-1-carboxylic acid as the sole nitrogen source. The eleven strains belonged to five different genera including Pantoea, Pseudomonas, Burkholderia, Acinetobacter and Ralstonia.
  
  
  CONCLUSIONS
  Phosphate-dissolving endophytic bacteria isolated from rape and maize plants have abundant characteristics relative to promoting plant growth and genetic diversity.
authors.affiliation: College of Life Science, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Xiafang
authors.initials: X
authors.lastname: Sheng
authors.forename: Linyan
authors.initials: L
authors.lastname: He
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D044822:Biodiversity; D029741:Brassica rapa / Q000254:growth & development / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D003313:Zea mays / Q000254:growth & development / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010710:Phosphates; D012336:RNA, Ribosomal, 16S