[Research of TGF-beta1 inducing lung adencarcinoma PC9 cells to mesenchymal cells transition].
OBJECTIVE
It has been proven that epithelial-mesenchymal transition (EMT) not only correlated with embryonic development but also could promote tumor invasion and metastasis. Transforming growth factor beta-1 (TGF-beta1) has been identified as the main inducer of tumor EMT. The aim of this study was to investigate the effects of TGF-beta1 on EMT and PI3K/AKT signaling pathway in lung adencarcinoma PC9 cells.


METHODS
Cultured PC9 cells were treated with different concentrations of TGF-beta1 for 48 h. The morphological changes were observed under phase-contrast microscopy; EMT relative marker protein changes were assessed by Western blot and immunoflurescence staining. In addition, the expression of AKT and P-AKT were also measured by Western blot.


RESULTS
The data showed that TGF-beta1 could induce PC9 morphological alteration from epithelial to mesenchymal and upregulate the expression of mesenchymal maker protein Fibronectin. Obviously, the expression of P-AKT was downregulated by TGF-beta1 treatment for 48 h.


CONCLUSIONS
TGF-beta1 might induce EMT of PC9 cells, accompanied by the changes of PI3K/AKT signaling pathway.
Zhang
Huijun
H
Department of Pneumosurgery, Shanghai Pulmonary Hospital, Shanghai, 200433, China.
Zhang
Lei
L
Wang
Heyong
H
Chen
Xiaofeng
X
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008648:Mesoderm / Q000378:metabolism / Q000473:pathology; D008858:Microscopy, Phase-Contrast; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D005353:Fibronectins; D053773:Transforming Growth Factor beta1; D051057:Proto-Oncogene Proteins c-akt