title: The novel responses of ethambutol against Mycobacterium smegmatis mc²155 Revealed by proteomics analysis.
abstract: Ethambutol (EMB), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study. We compared the protein profiles on M. smegmatis mc²155 treated by EMB and untreated using fluorescence difference two-dimensional gel electrophoresis (2-D DIGE). A total of 40 differential protein spots were selected and 22 proteins were identified by HPLC-nano ESI-MS/MS analysis, including 16 over-expressed proteins and 6 under-expressed proteins. These proteins mainly affected energy metabolism, as well as synthesis and modification of macromolecules. The expressions of correspondent genes were confirmed by RT-PCR. This investigation provided some clues for searching potential drug targets.
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, 116044, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Zhan
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Sun
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.forename: Shizhu
authors.initials: S
authors.lastname: Zang
authors.forename: Yufang
authors.initials: Y
authors.lastname: Ma
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004977:Ethambutol / Q000494:pharmacology*; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D020543:Proteome / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D020543:Proteome; D004977:Ethambutol