title: Modified agarose gel for high-performance Southern blotting of short DNA fragments.
abstract: Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolved fractionation of short DNA fragments comparable to that from a high-concentration agarose gel could be obtained using a low-concentration agarose gel with a small zone of high-concentration agarose, and that the resulting DNA transfer is highly efficient and rapid.
authors.affiliation: Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Huang
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Fang-Zhen
authors.initials: FZ
authors.lastname: Sun
mesh_terms: D000818:Animals; D015139:Blotting, Southern / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009707:Nucleosomes / Q000378:metabolism
chemical_list: D009707:Nucleosomes; D004247:DNA