Identification of chosen apoptotic (TIAR and TIA-1) markers expression in thyroid tissues from adolescents with immune and non-immune thyroid diseases.
The aim of this study was to estimate sodium iodide symporter (NIS) and thyroid peroxidase (TPO) expression in thyrocytes from patients with GD and no-toxic multinodular goitre (NTMG) in relationship with apoptotic (TIAR and TIA-1) markers. The investigation was performed on thyroid cells isolated from postoperation thyroid tissues from 15 patients aged 12-21 years old with GD and 15 cases aged 13-21 years old with NTMG. Detection of NIS and TPO was performed by immunohistochemistry. Analysis of apoptotic markers in thyroid tissues was performed using antibodies to TIAR and TIA-1 by Western Blot and immunohistochemistry. Identification of proapoptotic TIAR and TIA-1 molecules in the thyroid tissues revealed a higher expression of both proteins in patients with Graves' disease (+++; +, respectively) in comparison to patients with NTNG (+; 0). In addition, TIAR expression was detected in three bands [p50, p42, p38 (kDa)] and TIA-1 in two bands [p22, p17 (kDa)]. using Western Blot test in patients with thyroid autoimmune diseases. In patients with NTNG expression of both apoptotic proteins was lower and identified in single bands: 42 (kDa) for TIAR and 17 (kDa) for TIA-1. The analysis of expression of NIS and TPO in thyroid follicular cells was higher in patients with Graves' disease in compared to their detection in patients with NTMG. In addition, degree of thyroid antigen expression positive correlated with amount of proapoptotic markers (TIAR, p<0.001; TIA-1, p<0.025 for NIS; TIAR, p<0.012 for TPO). We conclude that elevated expression of NIS and TPO in Graves' disease is associated with higher stimulation and activation of apoptosis in thyroid follicular cells during autoimmune process.
Bossowski
A
A
Department of Paediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University in Bialystok, Poland. abossowski@hotmail.com
Czarnocka
B
B
Bardadin
K
K
Moniuszko
A
A
Łyczkowska
A
A
Czerwinska
J
J
Dadan
J
J
Bossowska
A
A
D000293:Adolescent; D017209:Apoptosis / Q000502:physiology*; D001323:Autoantibodies / Q000097:blood; D001327:Autoimmune Diseases / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism*; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007453:Iodide Peroxidase / Q000378:metabolism; D008297:Male; D039102:Poly(A)-Binding Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism; D000074019:T-Cell Intracellular Antigen-1; D013959:Thyroid Diseases / Q000378:metabolism* / Q000473:pathology; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D000099209:Sodium-Iodide Symporters
D001323:Autoantibodies; D015415:Biomarkers; D007453:Iodide Peroxidase; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; D027981:Symporters; D000074019:T-Cell Intracellular Antigen-1; D000099209:Sodium-Iodide Symporters; C071558:TIA1 protein, human; C076751:TIAL1 protein, human