Convenient RNA synthesis using a phosphoramidite possessing a biotinylated photocleavable group.
A convenient RNA synthesis using a biotin-streptavidin interaction and a photocleavable protecting group is described. The biotinylated photocleavable group was introduced at the 2'-position of the uridine derivative. Using the phosphoramidite 12, we attempted the synthesis of a 21mer RNA, which is pure enough to show potent RNAi activity compared with a conventionally prepared and HPLC-purified 21mer RNA with the same sequence.
Tomaya
Kota
K
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Takahashi
Mayumi
M
Minakawa
Noriaki
N
Matsuda
Akira
A
D019921:Biotinylation; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry*; D055668:Photochemical Processes; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019809:Streptavidin / Q000737:chemistry; D014529:Uridine / Q000737:chemistry
D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA; D019809:Streptavidin; D014529:Uridine