[Registration of K(+)-equilibrium potential in myometrium cell plasma membrane and study of its modulation of NO(x) and H2O2, using flow cytometry method].
Prospects of the use of flow cytometry analysis for investigation of forming K(+)- equilibrium membrane potential on the experimental model of myometrium plasma membrane vesicles in the presence of valinomycine using potential-sensitive probe of DiOC6(3). Transmembrane potential magnitude corresponds to magnitude by Nernst's equation. H2O2 and NO2-, probably, increase permeability of membrane for K+ and result in potential dissipation. Given effect is not shown for sodium nitroprusside. The obtained results confirm an assumption as to enhancing the passive transport for K+ through sarcolemma under the action of these substances, that can lead to membrane repolarisation and decline of the level of its excitability.
Danylovych
H V
HV
Danylovych
Iu V
IuV
Horchev
V F
VF
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects*; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012508:Sarcolemma / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D014634:Valinomycin / Q000494:pharmacology
D007476:Ionophores; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D009599:Nitroprusside; D014634:Valinomycin; D006861:Hydrogen Peroxide; D011188:Potassium