Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach.
Bartonella henselae is an emerging gram-negative facultative intracellular pathogen transmitted via Ctenocephalides felis (cat fleas) or cat scratches. Bartonellosis is present mainly in the form of cat scratch disease (CSD), bacillary angiomatosis and infective endocarditis (IE). The methods used to diagnose B. henselae rely on culturing, immunofluorescent assays and molecular techniques. The objective of the present study was to identify candidate proteins for the serodiagnosis of bartonellosis with the differential discrimination of both clinical scenarios: CSD and IE. For this, an immunoproteomic approach combined with 2-DE, immunoblotting and matrix-assisted laser desorption/ionization time-of-flight MS has been developed. Immunoproteomic profiles of sera collected from patients with CSD and IE were compared with those of blood donors. We identified several candidate proteins as phage-encoding Pap31 protein and an outer membrane protein of BH11510 that, in our view, might be useful for the serodiagnosis of bartonellosis.
Saisongkorh
Watcharee
W
URMITE CNRS-IRD UMR6236, IFR 48, Faculté de Médecine et de Pharmacie, Marseille, France.
Kowalczewska
Malgorzata
M
Azza
Saïd
S
Decloquement
Philippe
P
Rolain
Jean-Marc
JM
Raoult
Didier
D
D016917:Angiomatosis, Bacillary / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D018416:Bartonella henselae / Q000235:genetics / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D016022:Case-Control Studies; D002372:Cat-Scratch Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D040901:Proteomics; D012680:Sensitivity and Specificity
D001798:Blood Proteins