A promoter polymorphism -2122C>T of CHI3L1 is associated with serum low density lipoprotein cholesterol level in Korean subjects.
OBJECTIVE
This study assessed the effects of 10 tagging single nucleotide polymorphisms (SNPs) of the CHI3L1 gene on serum LDL cholesterol levels in 290 Korean subjects.


METHODS
Genotyping analyses of SNPs were conducted by TaqMan® method. The effects of the promoter SNP on mRNA expression and nuclear factor binding were measured by real-time PCR and electrophoretic mobility shift assay, respectively.


RESULTS
Among 10 tagging SNPs, -2122C>T SNP (rs946261) in the promoter region was significantly associated with serum LDL cholesterol level (P=0.005). The T allele of -2122C>T was associated with significantly increased mRNA expressions in peripheral blood cells of the subjects, and also increased a nuclear factor binding measured by an electrophoretic mobility shift assay.


CONCLUSIONS
-2122C>T of CHI3L1, a promoter SNP which affects the mRNA expression and nuclear factor binding, is significantly associated with serum LDL cholesterol levels in Korean subjects.
Lee
Haeyong
H
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Kang
Ryunhwa
R
Jee
Sun Ha
SH
Yoon
Yoosik
Y
D054392:Adipokines; D044466:Asian People / Q000235:genetics*; D020029:Base Pairing / Q000235:genetics; D000071451:Chitinase-3-Like Protein 1; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D055442:Metabolome / Q000235:genetics; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D056910:Republic of Korea
D054392:Adipokines; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D008078:Cholesterol, LDL; D006023:Glycoproteins; D037102:Lectins; D009687:Nuclear Proteins; D012333:RNA, Messenger