title: LAIR2 localizes specifically to sites of extravillous trophoblast invasion.
abstract: OBJECTIVE
  A global gene expression microarray analysis of surplus chorionic villus sampling (CVS) tissues identified leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) as down-regulated in the first trimester of pregnancies destined for preeclampsia. Neither the localization nor the function of LAIR2 has been examined in the placenta. Localization studies were conducted in placental tissues to determine the precise sites of LAIR2 mRNA production and protein binding.
  
  
  RESULTS
  Quantitative real time polymerase chain reaction (qRT-PCR) indicated LAIR2 expression in CVS, but none in breast, lymph node, kidney, skin, uterus, or third trimester placentas. In situ hybridization (ISH) revealed a highly restricted LAIR2 localization. LAIR2 mRNA was found only in the more distal portions of trophoblast anchoring cell columns, adjacent to the invading extravillous trophoblast (EVT). Immunohistochemistry (IHC) detected intracellular LAIR2 staining in these same cells. Extracellular staining of this soluble receptor was found in the acellular material between invasive EVT cells distal to the anchoring cell columns.
  
  
  CONCLUSIONS
  ISH and IHC staining for LAIR2 detected specific, highly localized expression at the leading edge of EVT anchoring cell columns in first trimester placentas. This staining likely identifies the site of production for this soluble receptor. Following secretion, the receptor appears to bind extracellular material among the invasive EVT. The precise restriction of this protein only to the sites of EVT invasion strongly suggests that it functions to regulate this invasion. The decreased LAIR2 expression noted in first trimester placentas that ultimately developed preeclampsia further suggests that alterations in LAIR2 may play an etiologic role in preeclampsia.
authors.affiliation: School of Nursing, University of Pittsburgh, Pittsburgh, PA, USA. foundss@pitt.edu
authors.forename: S A
authors.initials: SA
authors.lastname: Founds
authors.forename: B
authors.initials: B
authors.lastname: Fallert-Junecko
authors.forename: T A
authors.initials: TA
authors.lastname: Reinhart
authors.forename: Y P
authors.initials: YP
authors.lastname: Conley
authors.forename: W T
authors.initials: WT
authors.lastname: Parks
mesh_terms: D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D011225:Pre-Eclampsia / Q000209:etiology / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: C431503:LAIR-2 receptor; D011971:Receptors, Immunologic; D012313:RNA