Small interfering RNA (siRNA) against the survivin gene increases apoptosis in a canine melanoma cell line.
The effect of down-regulation of survivin expression by small interfering RNA (siRNA) against the canine survivin gene on apoptosis was investigated by transfecting MCM-N1 (a canine malignant oral melanoma cell line) cells with siRNA using cationic liposomes. The siRNA against the canine survivin gene induced an increase in the rate of apoptotic cells and a decrease in the number of viable cells. We also measured sequence-specific down-regulation of survivin expression by reverse transcription-PCR and western blot analysis. The siRNA directed against survivin reduced both mRNA and protein expression in MCM-N1 cells. These findings suggest the importance of survivin in canine melanoma tumors for inducing apoptosis, and reinforce the possibility of using survivin as a putative therapeutic target in canine malignant melanoma tumor.
Moriyama
Miyuki
M
Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Kano
Rui
R
Maruyama
Haruhiko
H
Hasegawa
Atsuhiko
A
Kamata
Hiroshi
H
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D008545:Melanoma / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering
D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering