title: Bat coronaviruses and experimental infection of bats, the Philippines.
abstract: Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.
authors.affiliation: University of Tokyo, Tokyo, Japan.
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Masangkay
authors.forename: Noriyo
authors.initials: N
authors.lastname: Nagata
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Shuetsu
authors.initials: S
authors.lastname: Fukushi
authors.forename: Phillip
authors.initials: P
authors.lastname: Alviola
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Naoya
authors.initials: N
authors.lastname: Ueda
authors.forename: Koichiro
authors.initials: K
authors.lastname: Iha
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Hikaru
authors.initials: H
authors.lastname: Fujii
authors.forename: Shumpei
authors.initials: S
authors.lastname: Tsuda
authors.forename: Maiko
authors.initials: M
authors.lastname: Endoh
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
mesh_terms: D000818:Animals; D001483:Base Sequence; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase