title: Antioxidant and hepatoprotective activities of five eggplant varieties.
abstract: Eggplant is consumed throughout the world and varies in fruit color, shape, and size. In this study, five varieties of eggplant (purple colored moderate size, white-green colored moderate size, long green, green striped moderate size and pale-green colored small size, respectively, called SM1-SM5) were evaluated for total phenolic and flavonoid content, antioxidant activity and hepatoprotection against cytotoxicity of tert-butyl hydroperoxide (t-BuOOH) in human hepatoma cell lines, HepG2. Total phenolic content found in methanol extracts of SM1-SM5 ranged from 739.36 ± 1.59 to 1116.13 ± 7.30 gallic acid equivalents mg/100g extract and total flavonoid content from 1991.29 ± 6.32 to 3954.20 ± 6.06 catechin equivalents mg/100 g extract. SM1 and SM2 which contained high total phenolic and flavonoid had better antioxidant activities than the other varieties. Pretreatment of HepG2 cells with 50 and 100 μg/mL of SM1-SM5 significantly increased the viability (p<0.05) of t-BuOOH-exposed HepG2 cells by 14.49 ± 1.14% to 44.95 ± 2.72%. The antioxidant activities of the eggplant were correlated with the total amounts of phenolic and flavonoid (r = 0.5310-0.7961). Significant correlation was found between hepatoprotective activities and total phenolic/flavonoid content (r = 0.6371-0.8842) and antioxidant activities (r = 0.5846-0.9588), indicating the contribution of the phenolic antioxidant present in eggplant to its hepatoprotective effect on t-BuOOH-induced toxicity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand. phpannak@mail2.ubu.ac.th
authors.forename: Pannarat
authors.initials: P
authors.lastname: Akanitapichat
authors.forename: Kallayanee
authors.initials: K
authors.lastname: Phraibung
authors.forename: Kwunchai
authors.initials: K
authors.lastname: Nuchklang
authors.forename: Suparichart
authors.initials: S
authors.lastname: Prompitakkul
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D006801:Humans; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D032323:Solanum melongena / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry; D013785:Thailand; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors / Q000633:toxicity
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D020122:tert-Butylhydroperoxide; C004931:1,1-diphenyl-2-picrylhydrazyl