title: Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress.
abstract: The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2',7'-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.
authors.affiliation: School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si 690-756, South Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: In Kyung
authors.initials: IK
authors.lastname: Lee
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.forename: Sang Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.forename: Bum Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011506:Proteins / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005960:Glucosides; D047348:Hydrolyzable Tannins; D011506:Proteins; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C024603:Geraniin