title: Developmental changes of cell adhesion molecule expression in the fetal mouse liver.
abstract: Developmental changes of cell adhesion molecule expression, especially in nonparenchymal cells, have hardly ever been analyzed in the murine liver. The present study was undertaken to immunohistochemically examine the expression of NCAM, ICAM, VCAM, and N-cadherin during mouse liver development and in fetal liver cell cultures. NCAM was transiently expressed in mesenchymal cells of the septum transversum and sinusoidal cells in liver development. In vitro studies demonstrated that desmin-positive stellate cells expressed this cell adhesion molecule. NCAM expression in periportal biliary epithelial cells and connective tissue cells also coincided well with bile duct remodeling processes in the perinatal periods. Expression of ICAM and VCAM was transiently restricted to hepatoblasts, hepatocytes and hemopoietic cells in fetal stages. N-cadherin was expressed not only in hepatoblasts and hepatocytes, but also in nonparenchymal cells such as endothelial cells, stellate cells and connective tissue cells, however the expression was weak. These results suggest that each cell adhesion molecule may play an important role during development in hepatic histogenesis, including hepatoblast/hepatocyte-stellate cell interactions, hemopoiesis, and bile duct morphogenesis.
authors.affiliation: Department of Biology, Faculty of Science, Shizuoka University, Shizuoka City, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Toru
authors.initials: T
authors.lastname: Koike
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shiojiri
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001652:Bile Ducts / Q000166:cytology / Q000196:embryology; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D047109:Fetal Development / Q000502:physiology*; D005333:Fetus / Q000196:embryology* / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules