title: A gigaseal obtained with a self-assembled long-lifetime lipid bilayer on a single polyelectrolyte multilayer-filled nanopore.
abstract: A lipid bilayer with gigaohm resistance was fabricated over a single 800 nm pore in a Si3N4 chip using 50 nm liposomes. The nanopore was prefilled with a polyelectrolyte multilayer (PEM) that triggered the spontaneous fusion of the lipid vesicles. Pore-forming peptide melittin was incorporated in the bilayer, and single channel activities were monitored for a period of 2.5 weeks. The long lifetime of the system enabled the observation of the time-dependent stabilization effect of the melittin open state upon bias application.
authors.affiliation: Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, CH-8092 Zurich, Switzerland.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugihara
authors.forename: János
authors.initials: J
authors.lastname: Vörös
authors.forename: Tomaso
authors.initials: T
authors.lastname: Zambelli
mesh_terms: D000595:Amino Acid Sequence; D017097:Electric Impedance; D004573:Electrolytes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008555:Melitten / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012825:Silicon / Q000737:chemistry; D017655:Silicon Compounds / Q000737:chemistry
chemical_list: D004573:Electrolytes; D008051:Lipid Bilayers; D011108:Polymers; D017655:Silicon Compounds; D008555:Melitten; C032734:silicon nitride; D012825:Silicon