Electrochemical behavior of atomoxetine and its voltammetric determination in capsules.
In this work, the electrochemical behavior and the analytical application of atomoxetine, a selective noradrenaline reuptake inhibitor, are studied. Atomoxetine, studied by differential pulse voltammetry and cyclic voltammetry on a glassy carbon electrode, exhibited an anodic response in aqueous media with pH between 1.5 and 7. In non-aqueous medium (acetonitrile), the drug exhibited two irreversible oxidation peaks that are diffusion controlled. From chronocoulometric studies in acetonitrile, it was determined that each oxidation signal involves two and four electrons, respectively. For analytical purposes, a differential pulse voltammetry technique in 0.1 mol L(-1) perchloric acid was selected, which exhibited adequate figures of merit. The percent recovery was 96.6+/-1.2 and the detection and quantitation limits were 6.9 x 10(-5) and 1.0 x 10(-4) mol L(-1), respectively. Also, results indicate that excipients do not interfere with the oxidation signal of atomoxetine, which leads to the conclusion that the developed method is satisfactorily selective for atomoxetine quantification in pharmaceuticals with no prior separation or extraction necessary. Finally, the proposed voltammetric method was successfully applied to both the assay and the uniformity content of atomoxetine in capsules. For comparison, high-performance liquid chromatography analysis was also performed.
Pérez-Ortiz
M
M
Bioelectrochemistry Laboratory, Chemical and Pharmaceutical Sciences Faculty, University of Chile, P.O. Box 233, Santiago 1, Chile.
Muñoz
C
C
Zapata-Urzúa
C
C
Alvarez-Lueje
A
A
D000069445:Atomoxetine Hydrochloride; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D011437:Propylamines / Q000032:analysis* / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002214:Capsules; D011437:Propylamines; D000069445:Atomoxetine Hydrochloride