Methods for human embryonic stem cells derived cardiomyocytes cultivation, genetic manipulation, and transplantation.
A decade has passed since the initial derivation of human embryonic stem cells (hESC). The ensuing years have witnessed a significant progress in the development of methodologies allowing cell cultivation, differentiation, genetic manipulation, and in vivo transplantation. Specifically, the potential to derive human cardiomyocytes from the hESC lines, which can be used for several basic and applied cardiovascular research areas including in the emerging field of cardiac regenerative medicine, attracted significant attention from the scientific community. This resulted in the development of protocols for the cultivation of hESC and their successful differentiation toward the cardiomyocyte lineage fate. In this chapter, we will describe in detail methods related to the cultivation, genetic manipulation, selection, and in vivo transplantation of hESC-derived cardiomyocytes.
Arbel
Gil
G
The Bruce Rappaport Faculty of Medicine, Sohnis Laboratory for Cardiac Electrophysiology and Regenerative Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Caspi
Oren
O
Huber
Irit
I
Gepstein
Amira
A
Weiler-Sagie
Michal
M
Gepstein
Lior
L
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000628:therapy; D032383:Myocytes, Cardiac / Q000166:cytology*; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods