title: Hemoglobin binding activity and hemoglobin-binding protein of Prevotella nigrescens.
abstract: Prevotella nigrescens, lacking siderophores was found to bind to the hemoproteins. The binding was observed also in the envelope which was prepared by sonication of the cell. The binding occurred in the pH-dependent manner; the binding was observed below neutral pHs of the incubation mixtures but only slightly observed in the neutral and alkaline pHs. Furthermore, hemoglobin bound to the envelope was dissociated at high pHs buffers. Maximum amounts of hemoglobin bound to 1 mg envelope was 51.2 mug. Kd for the reaction at pH 5.0 was 2.1 x 10¹⁰ M (210 pM). From the dot blot assay, hemoglobin could bind to a protein solubilized from the envelope by a detergent, referred to as hemoglobin-binding protein (HbBP), then it was purified by the sequential procedures of ion exchange chromatography, affinity chromatography and isoelectric focusing. Molecular weight and isoelectric point of the HbBP were 46 kDa and 6.1, respectively.
authors.affiliation: Department of Oral Health Promotion, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri-Nagano, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyashita
authors.forename: S
authors.initials: S
authors.lastname: Oishi
authors.forename: A
authors.initials: A
authors.lastname: Kiso
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: O
authors.initials: O
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: Y
authors.initials: Y
authors.lastname: Shibata
authors.forename: Setsuo
authors.initials: S
authors.lastname: Fujimura
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006454:Hemoglobins / Q000737:chemistry; D045242:Prevotella nigrescens / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D006454:Hemoglobins; C017206:hemoglobin-binding protein, bacteria