Knock-out of metacaspase and/or cytochrome c results in the activation of a ROS-independent acetic acid-induced programmed cell death pathway in yeast.
To gain further insight into yeast acetic acid-induced programmed cell death (AA-PCD) we analyzed the effects of the antioxidant N-acetyl-L-cysteine (NAC) on cell viability, hydrogen peroxide (H(2)O(2)) production, DNA fragmentation, cytochrome c (cyt c) release and caspase-like activation in wild type (wt) and metacaspase and/or cyt c-lacking cells. We found that NAC prevents AA-PCD in wt cells, by scavenging H(2)O(2) and by inhibiting both cyt c release and caspase-like activation. This shows the occurrence of a reactive oxygen species (ROS)-dependent AA-PCD. Contrarily no NAC dependent change in AA-PCD of mutant cells was detectable, showing that a ROS-independent AA-PCD can also occur.
Guaragnella
Nicoletta
N
CNR, Istituto di Biomembrane e Bioenergetica, Via Amendola 165/A, 70126 Bari, Italy.
Passarella
Salvatore
S
Marra
Ersilia
E
Giannattasio
Sergio
S
D019342:Acetic Acid / Q000494:pharmacology; D000111:Acetylcysteine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D061945:Caspase Inhibitors; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D055786:Gene Knockout Techniques; D005800:Genes, Fungal; D008954:Models, Biological; D009154:Mutation; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D000975:Antioxidants; D061945:Caspase Inhibitors; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid; D000111:Acetylcysteine