title: Synthesis and properties of triplex-forming oligonucleotides containing 2'-O-(2-methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine.
abstract: 2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Lou
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.forename: Lavinia
authors.initials: L
authors.lastname: Brennan
authors.forename: Mark E
authors.initials: ME
authors.lastname: Light
authors.forename: Nuria
authors.initials: N
authors.lastname: Vergara-Irigaray
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Atkinson
authors.forename: Lindy M
authors.initials: LM
authors.lastname: Holden-Dye
authors.forename: Keith R
authors.initials: KR
authors.lastname: Fox
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D018983:DNA Footprinting; D006801:Humans; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D014529:Uridine