Adipose tissue-derived stem cells from humans and mice differ in proliferative capacity and genome stability in long-term cultures.
Adipose tissue-derived stem cells (ASCs) are among the more attractive adult stem cell options for potential therapeutic applications. Here, we studied and compared the basic biological characteristics of ASCs isolated from humans (hASCs) and mice (mASCs) and maintained in identical culture conditions, which must be examined prior to considering further potential clinical applications. hASCs and mASCs were compared for immunophenotype, differentiation potential, cell growth characteristics, senescence, nuclear morphology, and DNA content. Although both strains of ASCs displayed a similar immunophenotype, the percentage of CD73(+) cells was markedly lower and CD31(+) was higher in mASC than in hASC cultures. The mean population doubling time was 98.08 ± 6.15 h for hASCs and 52.58 ± 3.74 h for mASCs. The frequency of nuclear aberrations was noticeably lower in hASCs than in mASCs regardless of the passage number. Moreover, as the cells went through several in vitro passages, mASCs showed changes in DNA content and cell cycle kinetics (frequency of hypodiploid, G0/G1, G2/M, and hyperdiploid cells), whereas all of these parameters remained constant in hASCs. Collectively, these results suggest that mASCs display higher proliferative capacity and are more unstable than hASCs in long-term cultures. These results underscore the need to consider specificities among model systems that may influence outcomes when designing potential human applications.
Danoviz
Maria Elena
ME
Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
Bassaneze
Vinícius
V
Nakamuta
Juliana Sanajotti
JS
dos Santos-Junior
Gabriel Ribeiro
GR
Saint-Clair
Danilo
D
Bajgelman
Márcio Chaim
MC
Faé
Kellen Cristhina
KC
Kalil
Jorge
J
Miyakawa
Ayumi Aurea
AA
Krieger
José Eduardo
JE
D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002454:Cell Differentiation; D053144:Cell Nucleus Shape; D049109:Cell Proliferation; D016922:Cellular Senescence; D005434:Flow Cytometry; D042822:Genomic Instability; D006801:Humans; D051379:Mice; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology; D010641:Phenotype; D011003:Ploidies; D013045:Species Specificity; D013997:Time Factors
D015703:Antigens, CD