GluR2/3 label expression of the AMPA-type glutamate receptor in the hippocampal formation of the homing pigeon stabilizes just after birth.
The compositions of the glutamate AMPA-type receptors influence the neural response and the subunits GluR2/3 has been referred to as essential for receptor trafficking and synapse consolidation. We investigate the GluR2/3 occurrence and expression in the hippocampal formation of newly born homing pigeons by a semi-quantitative approach, the Western-blotting technique and by immunohistochemistry. Immunoreactivity for GluR2/3 occurs before hatching has been evident in neuropil that was fully dispersed over the hippocampus proper (HP) and the area parahippocampalis (APH). Although many HP cells are NeuN-positives, a specific neuronal protein indicating that they are already differentiated as neurons while not one contains GluR2/3 at the hatching day (P0). Few neurons at the APH seem to express GluR2/3 at P0, but 3 days later (P3) the GluR2/3 labeling can be recognized in many HP neurons, showing a distribution pattern that resembles the adult, gradually increasing in intensity until P10. Also, the Western-blot shows an augment between P0 and P3, remaining stable after that. The enhancement of the neuronal label at P3 coincides with the retraction of the GluR2/3 label in neuropil, reducing their occurrence during the maturational period to become restricted to the dorsomedial portion as reported for adults. As the HP GluR2/3-containing cells are supposedly projecting neurons, taking together, the results signalize the relevance of the GluR2/3 in post-hatch formation of avian hippocampal circuitry in which the third day seems to be the critical period.
Cabral
Ana Lucia Beirão
AL
Núcleo de Pesquisa em Neurociências, Universidade Cidade de São Paulo, 03071-000 São Paulo, SP, Brazil.
Santana
Renato Figueiredo
RF
da Silva
Vinicius Otavio
VO
de Toledo
Cláudio Antonio Barbosa
CA
D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D010856:Columbidae / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D021122:Protein Subunits / Q000378:metabolism*; D018091:Receptors, AMPA / Q000378:metabolism*
D021122:Protein Subunits; D018091:Receptors, AMPA