title: Identifying genes progressively silenced in preneoplastic and neoplastic liver tissues.
abstract: High-throughput genomic technologies are increasingly being used to identify therapeutic targets and risk factors for specific diseases. Using 116 independent liver samples, we identified 793 probe sets that demonstrated a significant association in the frequency of absent calls as tissues progressed from normal to pre-neoplastic to neoplastic, followed by a bioinformatic approach which identified that 78.9% of the significant probe sets contained at least one CpG island in the gene promoter region compared with 58.9% of the remaining genes examined. Our results indicate that further high-throughput methylation studies to more fully characterize molecular events involved in hepatocarcinogenesis are warranted.
authors.affiliation: Department of Biostatistics, Virginia Commonwealth University, Richmond, Virginia 23298-0032, USA. kjarcher@vcu.edu
authors.forename: Kellie J
authors.initials: KJ
authors.lastname: Archer
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Zhao
authors.forename: Tobias
authors.initials: T
authors.lastname: Guennel
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Maluf
authors.forename: Robert A
authors.initials: RA
authors.lastname: Fisher
authors.forename: Valeria R
authors.initials: VR
authors.lastname: Mas
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology / Q000379:methods; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D023281:Genomics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D011230:Precancerous Conditions / Q000235:genetics; D011401:Promoter Regions, Genetic; D012307:Risk Factors