title: Identification and characterization of uncoupling protein in heart and muscle mitochondria of canary birds.
abstract: An uncoupling protein (cUCP) was identified in heart and skeletal muscle mitochondria of canary birds. cUCP was immunodetected using polyclonal antibodies raised against murine UCP2. Its molecular mass was similar to those of mammalian UCPs (32 kDa). The activity of cUCP was stimulated by palmitic acid (PA) and inhibited by GTP mainly in state 3 respiration. Additions of PA augmented state 4 respiration and lowered the ADP/O ratio. Thus, the activity of cUCP diverted energy from oxidative phosphorylation in state 3 respiration. cUCP in heart and skeletal muscles of canary birds might have implications in thermogenesis as well as protection against free radical production.
authors.affiliation: Department of Animal Physiology and Development, Adam Mickiewicz University, Poznań, Poland. slocina@amu.edu.pl
authors.forename: Malgorzata B
authors.initials: MB
authors.lastname: Slocinska
authors.forename: Zakaria Ali Moh
authors.initials: ZA
authors.lastname: Almsherqi
authors.forename: Francis E
authors.initials: FE
authors.lastname: Sluse
authors.forename: Rachel
authors.initials: R
authors.lastname: Navet
authors.forename: Yuru
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000818:Animals; D002171:Canaries / Q000378:metabolism*; D007473:Ion Channels / Q000032:analysis / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1