title: Antimycobacterial activity of Indigofera suffruticosa with activation potential of the innate immune system.
abstract: Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The antimycobacterial activity and the innate immune response of methanol (METH) and dichloromethane (DCM) extracts of Indigofera suffruticosa Miller (Fabaceae) were evaluated. We observed that the minimum inhibitory concentrations (MICs) for METH and DCM extracts were 125 and 1000 microg/mL, respectively. However, they were able to induce the innate immune response through the production of high levels of NO and TNF-alpha (p < 0.001) by peritoneal exudate cells (PECs). These results suggest that I. suffruticosa extracts may have an important immunological role in the control of TB once macrophage activity is induced by them.
authors.affiliation: Departamento de Análises Clínicas e Departamento de Ciências Biológicas, UNESP, R. Expedicionariosdo Brasil 1621, 14801-902 Araraquara, SP, Brazil. camilabac@yahoo.com.br
authors.forename: Camila B
authors.initials: CB
authors.lastname: de A Carli
authors.forename: Marcela B
authors.initials: MB
authors.lastname: Quilles
authors.forename: Daniele C G
authors.initials: DC
authors.lastname: Maia
authors.forename: Flávia C M
authors.initials: FC
authors.lastname: Lopes
authors.forename: Rubens
authors.initials: R
authors.lastname: Santos
authors.forename: Fernando R
authors.initials: FR
authors.lastname: Pavan
authors.forename: Clarice Q
authors.initials: CQ
authors.lastname: Fujimura Leite
authors.forename: Tamara R
authors.initials: TR
authors.lastname: Calvo
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Iracilda Z
authors.initials: IZ
authors.lastname: Carlos
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology; D029883:Indigofera; D051379:Mice; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000276:immunology; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts