Development of a (PQQ)-GDH-anode based on MWCNT-modified gold and its application in a glucose/O2-biofuel cell.
In this study a biofuel cell anode is developed on the basis of multi-walled carbon nanotubes (MWCNTs). Recombinant pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase (GDH) is covalently coupled to a PQQ-layer which is adsorbed onto thiol-modified MWCNTs at a gold electrode. In the presence of glucose a catalytic current starts at a potential of -80 mV vs. Ag/AgCl, 1M KCl. Under substrate saturation current densities of 170-200 μA/cm2 can be achieved. The operation is based on mediated electron transfer of the enzyme. This (PQQ)-GDH-MWCNT-electrode is combined with a MWCNT-modified electrode to which bilirubin oxidase (BOD) is covalently coupled. The resulting membrane-free biofuel cell has an open cell potential of 600 mV and can achieve a power density in the range of 23 μW/cm2.
Tanne
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Biosystems Technology, University of Applied Sciences Wildau, Bahnhofstraße, D-15745 Wildau, Germany.
Göbel
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Lisdat
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D001674:Bioelectric Energy Sources; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000737:chemistry*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry*; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010100:Oxygen / Q000737:chemistry*
D037742:Nanotubes, Carbon; D006046:Gold; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D005947:Glucose; D010100:Oxygen