[Protective effect of extractive of Dioscorea zingiberensis against ischemia/reperfusion injury in cultured neonatal rat cardiomyocytes].
OBJECTIVE
To define the structure of a water-soluble compound (SY-A) extracted from Dioscorea zingiberensis and investigate its protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion (I/R) injured.


METHODS
The SY-A was isolated from Dioscorea zingiberensis by utilizing column chromatograph and its structure was identified by 1H-NMR, 13C-NMR, 135DEPT. Neonatal rat cardiomyocytes were cultured and divided into 5 groups randomly. Then a model of ischemic/reperfusion (I/R) was established. Cells were divided into control group, simulated ischemia/reperfusion group (I/R group), I/R + SY-A (50 mg/L) group, I/ R + SY-A (100 mg/L) group and I/R + SY-A (150 mg/L) group. The levels of malondialdehyde and Creatine Kinase were measured. The cell viability was determined by MTT assay.


RESULTS
The structure of SY-A was identified as 4-methoxybenzoic acid-2-O-glucopyranoside. Compared with I/R group, the levels of LDH and CK were decreased significantly in the SY-A group, and the cell viability was increased obviously.


CONCLUSIONS
Extractive of Dioscorea zingiberensis showed protective effect against the neonatal rat cardiomyocytes ischemia/reperfusion injured.
Yu
Wen-Xi
WX
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Hu
Chang-Ying
CY
Wang
Yan-Ping
YP
Bao
Hui-Yan
HY
D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D027742:Dioscorea / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D012503:Saponins; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase