Xylanases of anaerobic fungus Anaeromyces mucronatus.
The anaerobic fungus Anaeromyces mucronatus KF8 grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage saccharides including cellulase, endoglucanase, xylanase, alpha-xylosidase, beta-xylosidase, alpha-glucosidase, beta-glucosidase, beta-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extracellular fractions, but production into the medium was prevalent with the exception of intracellular beta-xylosidase, chitinases, N-acetylglucosaminidase, and lipase. Xylanase activity was predominant among the polysaccharide hydrolases. Extracellular production of xylanase was stimulated by the presence of cellobiose and oat spelt xylan. Zymogram of xylanases of strain KF8 grown on different carbon sources revealed several isoforms of xylanases with approximate molar masses ranging from 26 to 130 kDa.
Novotná
Z
Z
Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic, 142 20, Prague 4, Czech Republic. novotna@iapg.cas.cz
Procházka
J
J
Simůnek
J
J
Fliegerová
K
K
D000693:Anaerobiosis; D002475:Cellobiose / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification; D008970:Molecular Weight; D020085:Neocallimastigales / Q000201:enzymology* / Q000502:physiology; D014990:Xylans / Q000378:metabolism
D003470:Culture Media; D005656:Fungal Proteins; D014990:Xylans; D002475:Cellobiose; D002482:Cellulose; D006026:Glycoside Hydrolases