title: A silicon nanowire-based electrochemical glucose biosensor with high electrocatalytic activity and sensitivity.
abstract: An electrochemical glucose biosensor was developed by immobilizing glucose oxidase (GOx) on an electrode decorated with a novel nanostructure, silicon nanowires (SiNWs) with in situ grown gold nanoparticles (AuNPs). The immobilized GOx displayed a pair of well-defined and quasi-reversible redox peaks with a formal potential (E(o ')) of -0.376 V in a phosphate buffer solution. The fabricated glucose biosensor has good electrocatalytic activity toward oxidation of glucose. In addition, such resultant SiNWs-based glucose biosensor possesses high biological affinity. Particularly, the apparent Michaelis-Mentan constant was estimated to be 0.902 mM, which is much smaller than the reported values for GOx at a range of nanomaterials-incorporated electrodes. Consequently, this novel SiNWs-based biosensor is expected to be a promising tool for biological assays (e.g., monitoring blood glucose).
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Shuit-Tong
authors.initials: ST
authors.lastname: Lee
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose; D012825:Silicon