Short-term UV-B and UV-C radiations preferentially decrease spermidine contents and arginine decarboxylase transcript levels of Synechocystis sp. PCC 6803.
To investigate the short term effect of ultraviolet (UV) radiations on changes in pigments and polyamine contents, Synechocystis sp. PCC 6803 cells after exposure to UV-radiation were extracted by dimethylformamide and perchloric acid for pigments and polyamines determination, respectively. Cell growth was slightly decreased after 1 h exposure to UV-A and UV-B radiations. UV-C had little effect on cell growth despite the decrease of photosynthetic rate by about 18%. UV-A and UV-B decreased the contents of chlorophyll a and carotenoids whereas UV-C decreased chlorophyll a but had no effect on carotenoids. Spermidine contents were unaffected by UV-A, in contrast to the reduction of 25 and 50% by UV-B and UV-C, respectively. All three types of UV-radiation particularly reduced perchloric acid-insoluble spermidine. Importantly, putrescine and spermine which accounted for less than 1% of intracellular polyamines were increased by about three- to eight-fold by UV-B and UV-C, respectively. The changes in polyamines contents by UV-B and UV-C were consistent with the changes in transcript levels of arginine decarboxylase mRNA, but not with the protein levels. The decrease in the transcripts of adc2 but not adc1 was observed with UV-B and UV-C treatments.
Jantaro
Saowarath
S
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Pothipongsa
Apiradee
A
Khanthasuwan
Suparaporn
S
Incharoensakdi
Aran
A
D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D011700:Putrescine / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D013095:Spermidine / Q000032:analysis*; D013096:Spermine / Q000032:analysis; D046939:Synechocystis / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays
D012333:RNA, Messenger; D002734:Chlorophyll; D013096:Spermine; D002338:Carotenoids; D002262:Carboxy-Lyases; C019722:arginine decarboxylase; D013095:Spermidine; D011700:Putrescine