title: Detection of microRNAs in dried serum blots.
abstract: We observed the preservation of microRNAs in unrefrigerated dried serum blots. Preservation was not adversely affected by drying or storing at 37, 45, or 60°C instead of room temperature, but it was harmed when blots were dried incompletely before storage. Preservation of microRNAs in serum was not diminished if, instead of being kept frozen at -80°C, it was stored as dried blots at room temperature for 5 months or at 37°C for 4 weeks. Thus, dried blots can be a convenient and safer way to save, transport, and store serum for microRNA assays.
authors.affiliation: Department of Thoracic Surgery, Roswell Park Cancer Institute, Buffalo, NY 14203, USA. santosh.patnaik@roswellpark.org
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Patnaik
authors.forename: Reema
authors.initials: R
authors.lastname: Mallick
authors.forename: Sai
authors.initials: S
authors.lastname: Yendamuri
mesh_terms: D005615:Freezing; D035683:MicroRNAs / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature
chemical_list: D035683:MicroRNAs