Resin technologies: construction and staining of resin TMA's.
The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tissue microarrays created from it, provide good morphology but with a compromised antigenicity when compared to frozen tissue. In contrast, while solving the issue of antigenicity, frozen tissue suffers from a lack of morphology. We have demonstrated that tissue microarrays constructed in glycol methacrylate resin, when combined with a cold acetone fixation step, have been able to combine the superior morphology of resin-embedded sections with the superior antigenicity of frozen tissue for prospectively collected material.
Howat
William J
WJ
Histopathology/ISH facility, Li Ka Shing Centre, Cancer Research UK, Cambridge Research Institute, Cambridge, UK. will.howat@cancer.org.uk
Wilson
Susan J
SJ
D000818:Animals; D005374:Filtration; D007150:Immunohistochemistry; D051379:Mice; D012117:Resins, Synthetic; D013194:Staining and Labeling / Q000379:methods*; D046888:Tissue Array Analysis / Q000379:methods*; D016610:Tissue Embedding
D012117:Resins, Synthetic