Apoptotic effects of polyprenylated benzoylphloroglucinol derivatives from the twigs of Garcinia multiflora.
With bioassay-guided fractionation, five new polyprenylated benzoylphloroglucinol derivatives, garcimultiflorone D (1), 18-hydroxygarcimultiflorone D (2), garcimultiflorone E (3), garcimultiflorone F (4), and isogarcimultiflorone F (5), and five known compounds, guttiferone E (6), guttiferone F (7), aristophenone A (8), isoxanthochymol (9), and morelloflavone (10), were isolated from the acetone extract of the twigs of Garcinia multiflora. The compounds were evaluated for their apoptotic effects against HeLa-C3 cells, which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. Compounds 1 and 3-9 activate caspase-3 in HeLa-C3 cells within 72 h after treatment at a concentration of 100 microM or lower. In particular, compounds 6, 8, and 9 showed strong apoptosis-inducing effects at a concentration of 25 microM.
Liu
Xin
X
Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin, NT, Hong Kong, People's Republic of China.
Yu
Ting
T
Gao
Xue-Mei
XM
Zhou
Yan
Y
Qiao
Chun-Feng
CF
Peng
Yong
Y
Chen
Shi-Lin
SL
Luo
Kathy Qian
KQ
Xu
Hong-Xi
HX
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029760:Garcinia / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010696:Phloroglucinol