Live-cell super-resolution imaging with trimethoprim conjugates.
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.
Wombacher
Richard
R
Department of Chemistry, Columbia University, New York, NY, USA.
Heidbreder
Meike
M
van de Linde
Sebastian
S
Sheetz
Michael P
MP
Heilemann
Mike
M
Cornish
Virginia W
VW
Sauer
Markus
M
D002470:Cell Survival; D006367:HeLa Cells; D006657:Histones / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity; D014295:Trimethoprim / Q000737:chemistry*
D006657:Histones; D014295:Trimethoprim