Medicinal mushroom Ganoderma lucidum as a potent elicitor in production of t-resveratrol and t-piceatannol in peanut calluses.
Phytoalexins t-resveratrol and t-piceatannol, the well-known health-promoting active components in plants, are secondary metabolites generated upon biotic or abiotic stresses. We have reported UV-irradiated peanut callus is a potent means to produce these compounds (J. Agric. Food Chem. 2005, 53, 3877). In this work, the effects of fungi and chemical elicitors on induction of t-resveratrol and t-piceatannol were examined. Results showed the investigated fungi Botryodiplodia theobromae and Reishi Ganoderma lucidum were generally more effective than chemical stress methyl jasmonate, salicylic acid, and sucrose. As high as 15.46+/-9.85 microg of t-resveratrol and 6.93+/-2.03 microg of t-piceatannol could be elicited in each gram of callus by sterilized G. lucidum mycelium (80 mg). Although much more sterilized G. ludicum mycelia was required to induce similar level of t-resveratrol and t-piceatannol in comparison to the sterilized B. theobromae mycelia (1 mg), uptake of the G. ludicum mycelium may provide a variety of health-promoting effects. Our findings suggest G. ludicum mycelium-treated peanut callus is a good source of bioactive components.
Yang
Ming-Hua
MH
Department of Food Technology, Hungkuang University, 34 Chung-Chie Road, Shalu, Taichung 433, Taiwan.
Lin
Yi-Ju
YJ
Kuo
Chang-Hsin
CH
Ku
Kuo-Lung
KL
D010367:Arachis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D020940:Reishi / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*
D013267:Stilbenes; C041525:3,3',4,5'-tetrahydroxystilbene; D000077185:Resveratrol