DNA microarray analysis of signaling pathway in macrophages stimulated by lignin-carbohydrate complex from lentinus edodes mycelia (LEM) extract.
In order to investigate the action point of lignin-carbohydrate complex (Fr4) from Lentinus edodes mycelia extract, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. Among seven lignin-carbohydrate complex fractions, Fr4 showed the highest stimulatory activity of tumor necrosis factor production by mouse macrophage-like J774.1 cells, as well as its previously reported anti-HIV activity. Fr4 is composed of lignin precursors such as vanillic acid, syringic acid, p-coumaric acid and ferulic acid, with trace amounts of flavonoids and tannins, and negligible amount of lipopolysaccharides (LPS), confirming the authenticity of Fr4 as a lignin. DNA microarray analysis suggested that Fr4 may affect immune response-related gene expression; however, it may not affect the expression of as many genes as LPS does.
Kawano
Michiyo
M
MPL, Meikai University School of Dentistry, Sakado, Saitama, Japan.
Thet
May Maw
MM
Makino
Toru
T
Kushida
Tatsuya
T
Sakagami
Hiroshi
H
D000818:Animals; D002460:Cell Line; D016000:Cluster Analysis; D015870:Gene Expression / Q000187:drug effects; D008031:Lignin / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000032:analysis / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D025341:Principal Component Analysis; D020942:Shiitake Mushrooms / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D008031:Lignin