Effects of alcohol-drinking behaviour and ADH1B and ALDH2 polymorphisms on basal DNA damage in human mononuclear cells as determined by the comet assay.
The aim of this study was to investigate the effects of alcohol drinking and ADH1B and ALDH2 polymorphisms on basal DNA damage (measured by the alkaline comet assay) of mononuclear cells in 122 healthy Japanese workers. Our results showed that drinking frequency had a significant impact on the tail moment (TM) value, with the highest TM value observed in habitual drinkers. The presence of the ADH1B*2 or ALDH2*2 allele was associated with increased DNA damage in older habitual drinkers. Furthermore, habitual drinkers with a combined genotype of ADH1B*2/*2 and ALDH2*1/*2 demonstrated a significantly higher TM value than other groups. Moreover, the combination of drinking and smoking has a combined effect on DNA damage. Multiple regression analysis revealed that drinking frequency, smoking status, and ALDH2 polymorphisms significantly influence basal TM value, suggesting that these are important variables affecting individual basal DNA damage.
Weng
Huachun
H
Department of Environmental and Preventive Medicine, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Osaka 565-0871, Japan.
Weng
Zuquan
Z
Lu
Yuquan
Y
Nakayama
Kunio
K
Morimoto
Kanehisa
K
D000328:Adult; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000428:Alcohol Drinking / Q000235:genetics*; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D020552:Comet Assay; D004249:DNA Damage; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors
C489332:ADH1B protein, human; D000426:Alcohol Dehydrogenase; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial