title: A copper-containing oxidase catalyzes C-nitrosation in nitrosobenzamide biosynthesis.
abstract: Although C-nitroso aromatic compounds have several bioactivities of medicinal interest, the biosynthetic enzymes involved in C-nitrosation have remained unknown until now. Here, we report the entire biosynthesis pathway of 4-hydroxy-3-nitrosobenzamide in Streptomyces murayamaensis, in which a tyrosinase-like copper-containing monooxygenase is responsible for the C-nitrosation. This finding indicates diverse catalytic functions of tyrosinase-like copper-containing monooxygenases in nature.
authors.affiliation: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Noguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kitamura
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Onaka
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D001549:Benzamides / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D003300:Copper / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D015538:Nitrosation; D009603:Nitroso Compounds / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*
chemical_list: D001549:Benzamides; D009566:Nitrates; D009603:Nitroso Compounds; D003300:Copper; C076039:3-nitrosobenzamide; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C024748:copper oxidase