Copro-molecular identification of infections with hookworm eggs in rural Lao PDR.
The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%.
Sato
Megumi
M
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Ratchathewi, Bangkok, Thailand.
Sanguankiat
Surapol
S
Yoonuan
Tippayarat
T
Pongvongsa
Tiengkham
T
Keomoungkhoun
Malaythong
M
Phimmayoi
Inthava
I
Boupa
Boungnong
B
Moji
Kazuhiko
K
Waikagul
Jitra
J
D000293:Adolescent; D000328:Adult; D000723:Ancylostomatoidea; D000818:Animals; D002648:Child; D016054:DNA, Protozoan / Q000235:genetics; D005243:Feces / Q000469:parasitology*; D005260:Female; D006725:Hookworm Infections / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D007812:Laos / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012423:Rural Health; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D055815:Young Adult
D016054:DNA, Protozoan