title: [Effect of brominated furanones on biofilm formation of Escherichia coli on polyvinyl chloride material].
abstract: OBJECTIVE
  To study the influence of brominated furanones on the biofilm formation of Escherichia coli on the polyvinyl chloride (PVC) material, and to provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection.
  
  
  METHODS
  Three brominated furanones with representative chemical structure were chosen and coated on the surface modification of PVC materials, respectively [furanone 1: 3, 4-dibromo-5-hydroxy-furanone; furanone 2: 4-bromo-5-(4-methoxyphenyl)-3-(methylamino)-furanone; furanone 3: 3, 4-dibromo-5, 5-bis (4-methylphenyl)-2 (5H)-furanone]. All the modificated PVC materials and Escherichia coli were co-cultivated. The PVC material soaked with 75% ethanol for 5 minutes and Escherichia coli were co-cultivated together as the control group. The thickness of bacterial community and bacterial community quantity in the unit area on PVC materials were measured by confocal laser scanning microscope (CLSM), and the surface structure of biofilm formation was observed by scanning electron microscope (SEM).
  
  
  RESULTS
  The CLSM showed that the thickness of bacterial community and the bacterial community quantity in the unit area of PVC materials was significantly less (P < 0.05) in furanone 3 group than in control group, but no significant difference (P > 0.05) was found between furanone 1, furanone 2 groups and control group. SEM showed that the quantity of bacterial community in the unit area of PVC materials surface in furanone 3 group was fewer than that in control group at 6 hours; the biofilm structure on PVC materials surface formed at 18 hours in control group, furanone 1 group, and furanone 2 group, but there was no mature biofilm structure on PVC materials surface in furanone 3 group at 18 hours.
  
  
  CONCLUSIONS
  The impact of different brominated furanones on Escherichia coli biofilm formation on the surface of PVC materials is different, 3, 4-dibromo-5, 5-bis (4-methylphenyl)-2 (5H)-furanone can inhibit Escherichia coli biofilm formation on the surface of PVC material. Polyvinyl chloride
authors.affiliation: Department of Thoracic Surgery, Lung Cancer Research Centre of the Cancer Institute of Yunnan Province, Kunming Yunnan, 650118, P R China.
authors.forename: Lianhua
authors.initials: L
authors.lastname: Ye
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Huang
authors.forename: Youquan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guangqiang
authors.initials: G
authors.lastname: Zhao
authors.forename: Yujie
authors.initials: Y
authors.lastname: Lei
mesh_terms: D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects*; D005663:Furans / Q000494:pharmacology*; D054879:Halogenation; D011143:Polyvinyl Chloride / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005663:Furans; D011143:Polyvinyl Chloride