Effects of lead exposure on nitric oxide-associated gene expression in the olfactory bulb of mice.
Lead (Pb) is known to have toxic effects on the brain; however, data regarding its specific toxic effects on the olfactory bulb are lacking. Therefore, we investigated the relationship between acute Pb exposure and alterations in gene expression associated with the nitric oxide signaling pathway in the olfactory bulb of mice. After administration of Pb (intraperitoneal injections of 1 or 10 mg/kg Pb(CH(3)CO(2))(2) · 3H(2)O once per day for 4 days), body weight, motor activity, and gene expression in the olfactory bulb of mice were examined. High doses of Pb resulted in significant decreases in body weight, but motor coordination was not significantly altered until 11 days after the end of Pb treatment. The expression patterns of dimethylarginine dimethylaminohydrolase 1 (Ddah1), superoxide dismutase 1 (Sod1), and superoxide dismutase (Ccs) were increased, whereas expression of the Stratifin (Sfn) gene was significantly decreased following treatment with 10 mg/kg Pb. The expression patterns of nitric oxide synthases at the mRNA and protein levels, however, were not significantly altered by treatment with 10 mg/kg Pb. These findings indicate that Pb-induced neurotoxicity may be modulated in part by the expression of Ddah1, Sod1, Ccs, and Sfn in the olfactory bulb.
Kim
Samki
S
Department of Public Health, Keimyung University, 1000 Shindang-dong, Daegu 704-701, Republic of Korea.
Hyun
Jiyoung
J
Kim
Hyunji
H
Kim
Younghee
Y
Kim
Eunju
E
Jang
Jungae
J
Kim
Kisok
K
D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D007854:Lead / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D009830:Olfactory Bulb / Q000187:drug effects* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D007854:Lead; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase