title: Pulling genetic RNA out of tobacco mosaic virus using single-molecule force spectroscopy.
abstract: RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Su
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Niu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhang
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D008968:Molecular Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D014027:Tobacco Mosaic Virus / Q000737:chemistry / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins