Neutron capture nuclei-containing carbon nanoparticles for destruction of cancer cells.
HeLa cells were incubated with neutron capture nuclei (boron-10 and gadolinium)-containing carbon nanoparticles, followed by irradiation of slow thermal neutron beam. Under a neutron flux of 6 x 10(11) n/cm(2) (or 10 min irradiation at a neutron flux of 1 x 10(9) n/cm(2) s), the percentages of acute cell death at 8 h after irradiation are 52, 55, and 28% for HeLa cells fed with BCo@CNPs, GdCo@CNPs, and Co@CNPs, respectively. The proliferation capability of the survived HeLa cells was also found to be significantly suppressed. At 48 h after neutron irradiation, the cell viability further decreases to 35 +/- 5% as compared to the control set receiving the same amount of neutron irradiation dose but in the absence of carbon nanoparticles. This work demonstrates "proof-of-concept" examples of neutron capture therapy using (10)B-, (157)Gd-, and (59)Co-containing carbon nanoparticles for effective destruction of cancer cells. It will also be reported the preparation and surface functionalization of boron or gadolinium doped core-shell cobalt/carbon nanoparticles (BCo@CNPs, GdCo@CNPs and Co@CNPs) using a modified DC pulsed arc discharge method, and their characterization by various spectroscopic measurements, including TEM, XRD, SQUID, FT-IR, etc. Tumor cell targeting ability was introduced by surface modification of these carbon nanoparticles with folate moieties.
Hwang
Kuo Chu
KC
Department of Chemistry, National Tsing Hua University, Hsinchu 30043, Taiwan, ROC. kchwang@mx.nthu.edu.tw
Lai
Po Dong
PD
Chiang
Chi-Shiun
CS
Wang
Pei-Jen
PJ
Yuan
Chiun-Jye
CJ
D001895:Boron / Q000008:administration & dosage / Q000627:therapeutic use*; D016754:Boron Neutron Capture Therapy / Q000379:methods; D002244:Carbon / Q000737:chemistry; D002470:Cell Survival / Q000528:radiation effects; D003035:Cobalt / Q000008:administration & dosage / Q000627:therapeutic use*; D005682:Gadolinium / Q000008:administration & dosage / Q000627:therapeutic use*; D006367:HeLa Cells; D006801:Humans; D007554:Isotopes / Q000008:administration & dosage / Q000627:therapeutic use; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000532:radiotherapy*; D017247:Neutron Capture Therapy / Q000379:methods*
D007554:Isotopes; D003035:Cobalt; D002244:Carbon; D005682:Gadolinium; D001895:Boron