Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.
Crystals of biological macromolecules often exhibit considerable inter-crystal and intra-crystal variation in diffraction quality. This requires the evaluation of many samples prior to data collection, a practice that is already widespread in macromolecular crystallography. As structural biologists move towards tackling ever more ambitious projects, new automated methods of sample evaluation will become crucial to the success of many projects, as will the availability of synchrotron-based facilities optimized for high-throughput evaluation of the diffraction characteristics of samples. Here, two examples of the types of advanced sample evaluation that will be required are presented: searching within a sample-containing loop for microcrystals using an X-ray beam of 5 microm diameter and selecting the most ordered regions of relatively large crystals using X-ray beams of 5-50 microm in diameter. A graphical user interface developed to assist with these screening methods is also presented. For the case in which the diffraction quality of a relatively large crystal is probed using a microbeam, the usefulness and implications of mapping diffraction-quality heterogeneity (diffraction cartography) are discussed. The implementation of these techniques in the context of planned upgrades to the ESRF's structural biology beamlines is also presented.
Bowler
Matthew W
MW
Structural Biology Group, European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, F-38043 Grenoble, France. bowler@esrf.fr
Guijarro
Matias
M
Petitdemange
Sebastien
S
Baker
Isabel
I
Svensson
Olof
O
Burghammer
Manfred
M
Mueller-Dieckmann
Christoph
C
Gordon
Elspeth J
EJ
Flot
David
D
McSweeney
Sean M
SM
Leonard
Gordon A
GA
D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray / Q000379:methods*; D008928:Mitochondria / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000737:chemistry; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000737:chemistry; D013820:Thermolysin / Q000032:analysis / Q000737:chemistry
D011943:Receptors, Adrenergic, beta; D013820:Thermolysin; D006180:Proton-Translocating ATPases