Spectral analysis and comparison of mineral deposits forming in opacified intraocular lens and senile cataractous lens.
This preliminary report was attempted to compare the chemical components of mineral deposits on the surfaces of an opacified intraocular lens (IOL) and a calcified senile cataractous lens (SCL) by vibrational spectral diagnosis. An opacified intraocular lens (IOL) was obtained from a 65-year-old male patient who had a significant decrease in visual acuity 2-years after an ocular IOL implantation. Another SCL with grayish white calcified plaque on the subcapsular cortex was isolated from a 79-year-old male patient with complicated cataract after cataract surgery. Optical light microscope was used to observe both samples and gross pictures were taken. Fourier transform infrared (FT-IR) and Raman microspectroscopic techniques were employed to analyze the calcified deposits. The curve-fitting algorithm using the Gaussian function was also used to quantitatively estimate the chemical components in each deposit. The preliminary results of spectral diagnosis indicate that the opacified IOL mainly consisted of the poorly crystalline, immature non-stoichiometric hydroxyapatite (HA) with higher content of type B carbonated apatites. However, the calcified plaque deposited on the SCL was comprised of a mature crystalline stoichiometric HA having higher contents of type A and type B carbonate apatites. More case studies should be examined in future.
Lin
Shan-Yang
SY
Department of Biotechnology, Yuanpei University, Taipei, Taiwan, People's Republic of China. sylin@mail.ypu.edu.tw
Chen
Ko-Hwa
KH
Lin
Chih-Cheng
CC
Cheng
Wen-Ting
WT
Li
Mei-Jane
MJ
D000180:Acrylic Resins; D000368:Aged; D000465:Algorithms; D001031:Apatites / Q000032:analysis*; D002114:Calcinosis / Q000378:metabolism*; D002130:Calcium Phosphates / Q000032:analysis*; D002386:Cataract / Q000378:metabolism*; D003460:Crystallization; D017886:Durapatite / Q000032:analysis*; D006801:Humans; D020100:Hydrogels; D007908:Lens, Crystalline / Q000737:chemistry; D007910:Lenses, Intraocular; D008297:Male; D019591:Pseudophakia / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D000180:Acrylic Resins; D001031:Apatites; D002130:Calcium Phosphates; D020100:Hydrogels; C022045:octacalcium phosphate; C006903:carbopol 940; C030782:carboapatite; D017886:Durapatite