title: Endothelialization of PVA/gelatin cryogels for vascular tissue engineering: effect of disturbed shear stress conditions.
abstract: Mechanically, poly(vinyl alcohol) (PVA)-based cryogels are extremely well suited for vascular tissue engineering applications. However, their surface properties lead to a slow rate of endothelialization, and the mode of cell attachment leaves the endothelium susceptible to removal under physiological shear stress conditions. In this study, abrupt and ramped disturbed shear stress conditions created by a turbulent orbital flow were used to examine endothelialization on PVA/gelatin cryogels. Cell proliferation rate and apoptosis were evaluated by fluorescent activated cell sorter (FACS) analysis, and the expression of cell-adhesion molecules was used to evaluate the response of cells on cryogels to static and shear conditions by real-time polymerase chain reaction (RT-PCR). Application of a ramped shear stress had a profound effect on endothelial cell proliferation (22.30 +/- 0.20-fold increase), necrosis (eliminated), apoptosis (1.04 +/- 0.18 increase), and overall facilitation of endothelialization while concomitantly increasing nitric oxide (NO) synthesis levels. Ramped shear stress was also effective in helping the retention of the endothelial cells on the cryogel surface, whereas abrupt application caused widespread removal. Under static conditions, Selectin-P expression decreased, whereas both inter-cellular adhesion molecule (ICAM) and platelet endothelial cell adhesion molecule (PECAM)-I expression increased on cryogels over a 10-day culture period. Under both shear stress conditions, Selectin-P expression was decreased both on cryogels and tissue culture polystyrene (TCPS). Controlled application of disturbed shear stress shortens endothelialization times on cryogel surfaces, in contrast to the established antiproliferative effect of shear stress caused by laminar flow, without compromising their functionality. This demonstrates how such mechanical stimuli can be exploited to alter cellular behavior and facilitate the required outcomes for tissue engineering applications.
authors.affiliation: School of Mechanical and Manufacturing Engineering, Materials Processing Research Centre, Dublin City University, Glasnevin, Dublin, Ireland.
authors.forename: Nihal Engin
authors.initials: NE
authors.lastname: Vrana
authors.forename: Paul A
authors.initials: PA
authors.lastname: Cahill
authors.forename: Garrett B
authors.initials: GB
authors.lastname: McGuinness
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D059985:Cryogels; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004727:Endothelium / Q000187:drug effects / Q000502:physiology*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D020100:Hydrogels / Q000494:pharmacology*; D007211:Indoles / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D059985:Cryogels; D020100:Hydrogels; D007211:Indoles; D009569:Nitric Oxide; C007293:DAPI; D005780:Gelatin; D011142:Polyvinyl Alcohol; D016650:Fluorescein-5-isothiocyanate