Mosaic zebrafish transgenesis for evaluating enhancer sequences.
The completion of the human genome sequence, along with that of many other species, has highlighted the challenge of ascribing specific function to non coding sequences. One prominent function carried out by the non coding fraction of the genome is to regulate gene transcription; however, there are no effective methods to broadly predict cis-regulatory elements from primary DNA sequence. We have developed an efficient protocol to functionally evaluate potential cis-regulatory elements through zebrafish transgenesis. Our approach offers significant advantages over cell-culture based techniques for developmentally important genes, since it provides information on spatial and temporal gene regulation. Conversely, it is faster and less expensive than similar experiments in transgenic mice, and we routinely apply it to sequences isolated from the human genome. Here we demonstrate our approach to selecting elements for testing based on sequence conservation and our protocol for cloning sequences and microinjecting them into zebrafish embryos.
Kague
Erika
E
Department of Cell and Developmental Biology, University of Pennsylvania-School of Medicine, PA, USA.
Weber
Christopher
C
Fisher
Shannon
S
D000818:Animals; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D006801:Humans; D051379:Mice; D008845:Microinjections / Q000379:methods; D009030:Mosaicism; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*