Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost.
A strictly aerobic, Gram-stain-negative, yellow-pigmented, non-spore-forming, motile (by gliding), rod-shaped bacterium, designated strain 15F3(T), was isolated from leaf-and-branch compost. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15F3(T) was most closely related to Flavobacterium reichenbachii WB 3.2-61(T) and formed a distinct phyletic lineage within the genus Flavobacterium, the type genus of the family Flavobacteriaceae. Growth was observed at 10-34 °C (optimum, 30 °C) and pH 6.0-8.0 (optimum, pH 7.0). No growth occurred in the presence of ≥2 % (w/v) NaCl. Strain 15F3(T) reduced nitrate to nitrogen and showed catalase activity but no oxidase activity. The predominant cellular fatty acids were iso-C(15 : 0) and summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH). The major isoprenoid quinone was menaquinone-6. The G+C content of the genomic DNA was 31.1 mol%. On the basis of data from this polyphasic study, strain 15F3(T) may be classified as a representative of a novel species within the genus Flavobacterium, for which the name Flavobacterium banpakuense sp. nov. is proposed; the type strain is 15F3(T) ( = KACC 14225(T)  = JCM 16466(T)).
Kim
Joong-Jae
JJ
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Jin
Hyun Mi
HM
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Lee
Hyo Jung
HJ
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Jeon
Che Ok
CO
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Kanaya
Eiko
E
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Koga
Yuichi
Y
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Takano
Kazufumi
K
CREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.|Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Kanaya
Shigenori
S
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D010858:Pigmentation; D018515:Plant Leaves; D018547:Plant Stems; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6