title: Genotypic analysis of plasmid-mediated beta-lactamases amongst Enterobacteriaceae other than Escherichia spp. and Klebsiella spp. that are non-susceptible to a broad-spectrum cephalosporin.
abstract: Enterobacteriaceae other than Escherichia spp. and Klebsiella spp. are recognisable for their increasing resistance. Plasmid-mediated beta-lactamases, including extended-spectrum beta-lactamases (ESBLs), plasmid-mediated AmpC beta-lactamases (pAmpCs) and the newly emerged KPC, play an important role in beta-lactam resistance. In this study, we investigated the genetic characteristics of plasmid-mediated beta-lactamases amongst non-Escherichia, non-Klebsiella Enterobacteriaceae that were non-susceptible to at least a broad-spectrum cephalosporin. A total of 143 (23.9%) of 598 isolates during the 6-month study period met the screening criteria, amongst which 142 (99.3%) and 99 (69.2%) isolates carried ESBL and pAmpC genes, respectively. Resistance genotypes were described. bla(KPC) was not detected in isolates with reduced susceptibility to carbapenems. This study provides an insight into plasmid-mediated resistance determinants amongst cephalosporin-non-susceptible Enterobacteriaceae other than Escherichia spp. and Klebsiella spp.
authors.affiliation: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok-Noi, Bangkok 10700, Thailand. sipkr@mahidol.ac.th
authors.forename: Pattarachai
authors.initials: P
authors.lastname: Kiratisin
authors.forename: Arunocha
authors.initials: A
authors.lastname: Henprasert
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D004925:Escherichia / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D002511:Cephalosporins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C063912:carbapenemase