High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications.
Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. In recent years, there has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of robust, fast, and cost-effective FLIM instrumentation suitable for in vivo tissue imaging. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of high-speed instruments for in vivo applications. We present a cost-effective scanning multispectral FLIM implementation capable of achieving pixel rates on the order of tens of kilohertz, which will facilitate the evaluation of FLIM for in vivo applications.
Shrestha
Sebina
S
Department of Biomedical Engineering, Texas A&M University, 337 Zachary Building, College Station, Texas 77843, USA.
Applegate
Brian E
BE
Park
Jesung
J
Xiao
Xudong
X
Pande
Paritosh
P
Jo
Javier A
JA
D000818:Animals; D006224:Cricetinae; D003952:Diagnostic Imaging / Q000379:methods; D004195:Disease Models, Animal; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D009062:Mouth Neoplasms / Q000473:pathology; D012815:Signal Processing, Computer-Assisted