Expression, crystallization and preliminary X-ray diffraction analysis of the CMM2 region of the Arabidopsis thaliana Morpheus' molecule 1 protein.
Of the known epigenetic control regulators found in plants, the Morpheus' molecule 1 (MOM1) protein is atypical in that the deletion of MOM1 does not affect the level of epigenetic marks controlling the transcriptional status of the genome. A short 197-amino-acid fragment of the MOM1 protein sequence can complement MOM1 deletion when coupled to a nuclear localization signal, suggesting that this region contains a functional domain that compensates for the loss of the full-length protein. Numerous constructs centred on the highly conserved MOM1 motif 2 (CMM2) present in these 197 residues have been generated and expressed in Escherichia coli. Following purification and crystallization screening, diamond-shaped single crystals were obtained that diffracted to approximately 3.2 A resolution. They belonged to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a=85.64, c=292.74 A. Structure determination is ongoing.
Petty
Tom J
TJ
Department of Molecular Biology, University of Geneva, Switzerland.
Nishimura
Taisuke
T
Emamzadah
Soheila
S
Gabus
Caroline
C
Paszkowski
Jerzy
J
Halazonetis
Thanos D
TD
Thore
Stéphane
S
D000074183:ATPases Associated with Diverse Cellular Activities; D020816:Amino Acid Motifs; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics
D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D000074183:ATPases Associated with Diverse Cellular Activities; C409208:MOM protein, Arabidopsis