title: Structural insights into substrate specificity and solvent tolerance in alcohol dehydrogenase ADH-'A' from Rhodococcus ruber DSM 44541.
abstract: The structure of the alcohol dehydrogenase ADH-'A' from Rhodococcus ruber reveals possible reasons for its remarkable tolerance to organic co-solvents and suggests new directions for structure-informed mutagenesis to produce enzymes of altered substrate specificity or improved selectivity.
authors.affiliation: Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50/3, A-8010 Graz, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Karabec
authors.forename: Andrzej
authors.initials: A
authors.lastname: Łyskowski
authors.forename: Katharina C
authors.initials: KC
authors.lastname: Tauber
authors.forename: Georg
authors.initials: G
authors.lastname: Steinkellner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
authors.forename: Gideon
authors.initials: G
authors.lastname: Grogan
authors.forename: Karl
authors.initials: K
authors.lastname: Gruber
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D000438:Alcohols / Q000138:chemical synthesis / Q000737:chemistry; D018360:Crystallography, X-Ray; D007659:Ketones / Q000737:chemistry; D008958:Models, Molecular; D011487:Protein Conformation; D012240:Rhodococcus / Q000201:enzymology*; D012997:Solvents / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D000438:Alcohols; D007659:Ketones; D012997:Solvents; D000426:Alcohol Dehydrogenase