Crystallization of zinc finger proteins bound to DNA.
A method is presented for determining conditions for the cocrystallization of zinc finger proteins with DNA. The method describes steps beginning with protein expression, through purification, design of DNA for cocrystallization, and the conditions to screen for cocrystallization.
Horton
Nancy C
NC
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ, USA.
Park
Chad K
CK
D000645:Ammonium Sulfate / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D004247:DNA; D000645:Ammonium Sulfate