title: Practical preparation of epilactose produced with cellobiose 2-epimerase from Ruminococcus albus NE1.
abstract: A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield.
authors.affiliation: Nihon Shokuhin Kako Co., Ltd, Tajima, Fuji, Shizuoka, Japan. wataru.saburi@nisshoku.co.jp
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Taguchi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hamada
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
mesh_terms: D002475:Cellobiose / Q000378:metabolism*; D002845:Chromatography; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D019998:Racemases and Epimerases / Q000378:metabolism*; D045857:Ruminococcus / Q000201:enzymology*
chemical_list: D004187:Disaccharides; D002475:Cellobiose; C047464:epilactose; D019998:Racemases and Epimerases