Roundup effects on oxidative stress parameters and recovery pattern of Rhamdia quelen.
Antioxidant enzymes and oxidative stress indicators were evaluated in fish exposed to different concentrations of the herbicide Roundup 48% (Monsanto, St. Louis, MO): control (none), 0.45, or 0.95 mg/l. After exposure for 8 days to herbicide, fish were transferred to clean water for a recovery response period (also 8 days). Herbicide increased thiobarbituric acid reactive species in liver and muscle at the higher concentration and in the brain at both concentrations. Protein carbonyl in liver increased after exposure. Catalase (CAT) and superoxide dismutase (SOD) activities and ascorbic acid levels in liver did not change in fish exposed to both concentrations. Glutathione S-transferase (GST) levels decreased at both concentrations. The nonprotein thiol levels decreased at the 0.95 mg/l concentration. During the recovery period, some of the parameters that had altered, such as protein carbonyl content, later recovered. However, some enzymes reacted during this period, e.g., GST increased its activity, possibly indicating a compensatory response against the toxic conditions. In contrast, CAT and SOD activities decreased during the recovery period, indicating herbicide toxicity. Oxidative stress that occurred during the exposure period was likely due to the increased lipid peroxidation and protein carbonyl content. The results concerning oxidative and antioxidant profiles indicate that short-term exposure to herbicide is capable of causing oxidative stress in fish tissues.
de Menezes
Charlene Cavalheiro
CC
Programa de Pós, Graduação em Bioquímica Toxicológica, Laboratório de Toxicologia de Pesticidas em Peixes, Universidade Federal de Santa Maria, Santa Maria, RS, 97105.900, Brasil.
da Fonseca
Milene Braga
MB
Loro
Vânia Lúcia
VL
Santi
Adriana
A
Cattaneo
Roberta
R
Clasen
Bárbara
B
Pretto
Alexandra
A
Morsch
Vera Maria
VM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002397:Catfishes / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000378:metabolism; D013858:Thiobarbiturates / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D000097797:Glyphosate
D000975:Antioxidants; D001205:Ascorbic Acid; D002374:Catalase; D005982:Glutathione Transferase; D005998:Glycine; D013482:Superoxide Dismutase; D013858:Thiobarbiturates; D014874:Water Pollutants, Chemical; D000097797:Glyphosate; C029684:thiobarbituric acid