title: Evaluation of a rapid immunodiagnostic test kit for detection of African lyssaviruses from brain material.
abstract: A rapid immunodiagnostic test kit was evaluated against a selection of isolates of lyssavirus genotypes occurring in Africa. The test was carried out in parallel comparison with the fluorescent antibody test (FAT) and isolates representing previously established phylogenetic groups from each genotype were included. The specificity of the rapid immunodiagnostic test compared favourably with the FAT and was found to detect all representatives of genotypes 1, 2, 3 and 4 in brain samples of either field cases or suckling mouse brain inoculates.
authors.affiliation: Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002 South Africa. wanda.markotter@up.ac.za
authors.forename: W
authors.initials: W
authors.lastname: Markotter
authors.forename: D
authors.initials: D
authors.lastname: York
authors.forename: C T
authors.initials: CT
authors.lastname: Sabeta
authors.forename: W
authors.initials: W
authors.lastname: Shumba
authors.forename: G
authors.initials: G
authors.lastname: Zulu
authors.forename: K
authors.initials: K
authors.lastname: Le Roux
authors.forename: L H
authors.initials: LH
authors.lastname: Nel
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology*; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary*; D005838:Genotype; D018114:Lyssavirus / Q000145:classification / Q000302:isolation & purification*; D051379:Mice; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary; D011820:Rabies virus / Q000302:isolation & purification; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*