title: Expression of human chemerin induces insulin resistance in the skeletal muscle but does not affect weight, lipid levels, and atherosclerosis in LDL receptor knockout mice on high-fat diet.
abstract: OBJECTIVE
  Chemerin is a recently discovered hepatoadipokine that regulates adipocyte differentiation as well as chemotaxis and activation of dendritic cells and macrophages. Chemerin was reported to modulate insulin sensitivity in adipocytes and skeletal muscle cells in vitro and to exacerbate glucose intolerance in several mouse models in vivo. In humans, chemerin was shown to be associated with multiple components of the metabolic syndrome including BMI, triglycerides, HDL cholesterol, and hypertension. This study aimed to examine the effect of chemerin on weight, glucose and lipid metabolism, as well as atherosclerosis in vivo.
  
  
  METHODS
  We used recombinant adeno-associated virus to express human chemerin in LDL receptor knockout mice on high-fat diet.
  
  
  RESULTS
  Expression of chemerin did not significantly alter weight, lipid levels, and extent of atherosclerosis. Chemerin, however, significantly increased glucose levels during the intraperitoneal glucose tolerance test without affecting endogenous insulin levels and the insulin tolerance test. Chemerin reduced insulin-stimulated Akt1 phosphorylation and activation of 5'AMP-activated protein kinase (AMPK) in the skeletal muscle, but had no effect on Akt phosphorylation and insulin-stimulated AMPK activation in the liver and gonadal adipose tissue.
  
  
  CONCLUSIONS
  Chemerin induces insulin resistance in the skeletal muscle in vivo. Chemerin is involved in the cross talk between liver, adipose tissue, and skeletal muscle.
authors: Department of Internal Medicine II, University of Munich, Munich, Germany.
authors: Melanie
authors: M
authors: Becker
authors: Katja
authors: K
authors: Rabe
authors: Corinna
authors: C
authors: Lebherz
authors: Julia
authors: J
authors: Zugwurst
authors: Burkhard
authors: B
authors: Göke
authors: Klaus G
authors: KG
authors: Parhofer
authors: Michael
authors: M
authors: Lehrke
authors: Uli C
authors: UC
authors: Broedl
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D001835:Body Weight; D018925:Chemokines / Q000097:blood / Q000235:genetics* / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008055:Lipids / Q000097:blood*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000502:physiology / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics; D011973:Receptors, LDL / Q000172:deficiency*
chemical_list: D001786:Blood Glucose; D018925:Chemokines; D004041:Dietary Fats; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D008055:Lipids; C478630:RARRES2 protein, human; D012333:RNA, Messenger; D011973:Receptors, LDL; D055372:AMP-Activated Protein Kinases