Photocatalytic activity of protein-conjugated CdS nanoparticles.
Colloidal CdS nanoparticles are conjugated with a variety of proteins, including enhanced yellow fluorescent protein, tobacco etch virus protease (TEV), lysozyme, and bacterial cytochrome P450 CYP152A1, and the photochemical properties of the resulting conjugates are analyzed by EPR spectroscopy and hydroxyl radical-specific fluorimetric assay. While irradiation of bare CdS colloids leads to photogeneration of hydroxyl and superoxide radicals, it is surprisingly observed that coating of the CdS particles with proteins effectively suppresses the production of these radical species and instead leads to increased formation of a long-lived reactive oxygen species, most likely H(2)O(2). A mechanism for the observed results is suggested. The empirical results are capitalized on for the assembly of a CdS-TEV nanohybrid, which shows significantly higher performance as a photocatalytic mediator for fatty acid hydroxylation by CYP152A1 than bare CdS nanoparticles.
Rajendran
Vidyalakshmi
V
Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Dortmund, Germany.
König
Anna
A
Rabe
Kersten S
KS
Niemeyer
Christof M
CM
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002384:Catalysis / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000737:chemistry*; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D010777:Photochemistry / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013431:Sulfates / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors
D001426:Bacterial Proteins; D019187:Cadmium Compounds; D055767:Immobilized Proteins; D008164:Luminescent Proteins; D011506:Proteins; D013431:Sulfates; C054562:yellow fluorescent protein, Bacteria; D003577:Cytochrome P-450 Enzyme System; C037123:cadmium sulfate; D010544:Peroxidases; C437763:cytochrome P-450 CYP152A1 (Bacillus subtilis); D009113:Muramidase; D010450:Endopeptidases; C099488:TEV protease