An antifungal defensin from Phaseolus vulgaris cv. 'Cloud Bean'.
An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 μM. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 μM. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 μM. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 μM and 40 μM, respectively.
Wu
Xiangli
X
State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China.
Sun
Jian
J
Zhang
Guoqing
G
Wang
Hexiang
H
Ng
Tzi Bun
TB
D000327:Adsorption; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D023082:Defensins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005658:Fungi / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D007938:Leukemia / Q000188:drug therapy; D008223:Lymphoma / Q000188:drug therapy; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy*; D027805:Phaseolus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds; D012685:Sepharose; D014227:Triazines
D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D023082:Defensins; D010936:Plant Extracts; D014227:Triazines; C012842:Cibacron Blue F 3GA; D012685:Sepharose