Polymorphism of glutathione S-transferase (GST) variants and its effect on distribution of urinary arsenic species in people exposed to low inorganic arsenic in tap water: an exploratory study.
Glutathione S-tranferases (GST) are multigenic enzymes that have been associated with arsenic metabolism. The objective of this study was to evaluate the relationship between polymorphic variants of GST and urinary concentration of arsenic species in people exposed to low levels of arsenic. A cross-sectional study among 66 nonoccupationally exposed subjects, living in the city of Antofagasta, Chile. Polymorphic variants were analyzed by polymerase chain reaction (PCR) and arsenic species was determined by atomic absorption spectrometry. The effect of GST variants on arsenic concentration was evaluated using univariate and covariate-adjusted regressions. For both GSTT1 and GSTM1 there were no significant differences in detected arsenic relative species between carriers of the active and null polymorphic variants. There was nondefinitive evidence that polymorphic variants of GST play a role in arsenic metabolism in sample of the Chilean subjects studied.
Caceres
Dante D
DD
División de Epidemiologia, Escuela de Salud Pública, Facultad de Medicina, Universidad de Chile, Santiago, Chile. dcaceres@med.uchile.cl
Werlinger
Fabiola
F
Orellana
Magdalena
M
Jara
Marcelo
M
Rocha
Rene
R
Alvarado
Sergio A
SA
Luis
Quiñones
Q
D001152:Arsenicals / Q000378:metabolism / Q000652:urine*; D002677:Chile; D003430:Cross-Sectional Studies; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D018709:Statistics, Nonparametric; D014881:Water Supply / Q000032:analysis*; D055815:Young Adult
D001152:Arsenicals; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C490476:GSTO1 protein, human