The association of genomic variation of Epstein-Barr virus BamHI F fragment with the proliferation of nasopharyngeal carcinoma.
To investigate the f variant of Epstein-Barr virus (EBV) in nasopharyngeal carcinogenesis, we detected the f variant in primary nasopharyngeal carcinoma (NPC), metastatic carcinoma of the lymph node (LN), and chronic inflammation of the nasopharynx from the Guangdong region. Meanwhile, we analyzed the relationship between the f variant of EBV and LMP1, Fascin, pStat3, p53, Bcl-2, and Ki-67 expression in NPC. The results showed that the f variant of EBV was found in 11 cases of primary NPCs with LN metastasis, 12 LN metastases, and 18 primary NPCs without LN metastasis. However, only one demonstrated the F/f variant in 50 cases of chronic inflammation of the nasopharynx. The expression rate of LMP1, Fascin, pStat3, p53, Bcl-2, and Ki-67 in NPC with the f or F/f variant was higher than that with the F prototype. Furthermore, there was a significantly positive correlation between the f variant of EBV and Ki-67 expression (p < 0.05). Our study suggests that the f variant of EBV may be closely related to nasopharyngeal carcinogenesis.
Liu
Qiuyu
Q
Department of Pathology, The First Affiliated Hospital and Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Han
Anjia
A
You
Shuyuan
S
Yang
Qingxu
Q
Liang
Yingjie
Y
Dong
Yu
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002352:Carrier Proteins / Q000235:genetics; D015245:Deoxyribonuclease BamHI / Q000235:genetics*; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000235:genetics; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D047992:TNF Receptor-Associated Factor 2 / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D002352:Carrier Proteins; D019394:Ki-67 Antigen; D008840:Microfilament Proteins; D010446:Peptide Fragments; D047992:TNF Receptor-Associated Factor 2; D014764:Viral Proteins; C020324:fascin; D015245:Deoxyribonuclease BamHI