title: Effect of chlorhexidine on bond strength of two-step etch-and-rinse adhesive systems to dentin of primary and permanent teeth.
abstract: OBJECTIVE
  To evaluate the influence of chlorhexidine digluconate (CHX) application on the immediate microtensile bond strength (microTBS) of two-step etch-and-rinse adhesive systems to the dentin of primary and permanent teeth.
  
  
  METHODS
  Noncarious human teeth (24 primary molars and 24 premolars) were used. The primary and permanent teeth were randomly assigned to three groups (n = 8) according to the adhesive system: Adper Single Bond, Prime & Bond NT and Excite DSC. Each group was further divided in two subgroups (n = 4) in which the phosphoric acid-etched dentin was treated with 20 microL of either 2% CHX or deionized water for 60 seconds prior to adhesive system application. The adhesive systems were applied according to the manufacturers' instructions and resin composite blocks were built up on the treated surfaces. The teeth were vertically sectioned perpendicular to adhesive interface and beam-shaped specimens with a 0.81 mm2 cross-sectional area were obtained and subjected to microTBS testing at a crosshead speed of 0.05 mm/minute. MicroTBS data were analyzed statistically by ANOVA and Tukey's test (alpha = 0.05). The failure modes were verified with a stereomicroscope.
  
  
  RESULTS
  CHX application increased significantly (P < 0.05) the microTBS of Prime & Bond NT and Single Bond to the acid-etched primary and permanent dentin, while no positive or negative effect was observed for Excite DSC. There was a predominance of adhesive failures in all control and CHX-treated groups. No fracture distribution pattern was observed.
authors: Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University, SP, Brazil.
authors: Hérica Adad
authors: HA
authors: Ricci
authors: Mariane Emi
authors: ME
authors: Sanabe
authors: Carlos Alberto de Souza
authors: CA
authors: Costa
authors: Josimeri
authors: J
authors: Hebling
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local; D017438:Bisphenol A-Glycidyl Methacrylate; D002710:Chlorhexidine; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin; D017220:Dentin-Bonding Agents; D019229:Dentition, Permanent; D006801:Humans; D008689:Methacrylates; D011109:Polymethacrylic Acids; D019279:Resin Cements; D013718:Tensile Strength; D014094:Tooth, Deciduous
chemical_list: D000891:Anti-Infective Agents, Local; D017220:Dentin-Bonding Agents; C420130:Excite cement; D008689:Methacrylates; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D002710:Chlorhexidine