title: Identification of a bacterial inhibitor against g-type lysozyme.
abstract: Lysozymes are antibacterial effectors of the innate immune system in animals that hydrolyze peptidoglycan. Bacteria have evolved protective mechanisms that contribute to lysozyme tolerance such as the production of lysozyme inhibitors, but only inhibitors of chicken (c-) and invertebrate (i-) type lysozyme have been identified. We here report the discovery of a novel Escherichia coli inhibitor specific for goose (g-) type lysozymes, which we designate PliG (periplasmic lysozyme inhibitor of g-type lysozyme). Although it does not inhibit c- or i-type lysozymes, PliG shares a structural sequence motif with the previously described PliI and MliC/PliC lysozyme inhibitor families, suggesting a common ancestry and mode of action. Deletion of pliG increased the sensitivity of E. coli to g-type lysozyme. The existence of inhibitors against all major types of animal lysozyme and their contribution to lysozyme tolerance suggest that lysozyme inhibitors may play a role in bacterial interactions with animal hosts.
authors: Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Centre, Katholieke Universiteit Leuven, Belgium.
authors: L
authors: L
authors: Vanderkelen
authors: J M
authors: JM
authors: Van Herreweghe
authors: K G A
authors: KG
authors: Vanoirbeek
authors: G
authors: G
authors: Baggerman
authors: B
authors: B
authors: Myrnes
authors: P J
authors: PJ
authors: Declerck
authors: I W
authors: IW
authors: Nilsen
authors: C W
authors: CW
authors: Michiels
authors: L
authors: L
authors: Callewaert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005777:Geese / Q000378:metabolism*; D008969:Molecular Sequence Data; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D020349:Surface Plasmon Resonance
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; C558304:PliG protein, E coli; D009113:Muramidase