Daidzein regulates secretion, production and gene expression of mucin from airway epithelial cells stimulated by proinflammatory factor and growth factor.
In this study, we investigated whether daidzein significantly affects secretion, production and gene expression of mucin from cultured airway epithelial cells. Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then chased for 30 min in the presence of daidzein to assess the effect on mucin secretion using ELISA. At the same time, confluent NCI-H292 cells were pretreated with daidzein for 30 min and then stimulated with EGF and PMA for 24 h, respectively. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) daidzein significantly decreased ATP-induced mucin secretion from cultured RTSE cells; (2) daidzein inhibited the production of MUC5AC mucin protein induced by EGF or PMA from NCI-H292 cells; (3) daidzein also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA from NCI-H292 cells. This result suggests that daidzein can regulate secretion, production and gene expression of mucin, by directly acting on airway epithelial cells.
Lee
Hyun Jae
HJ
Department of Pharmacology, College of Medicine, Chungnam National University, 6 Munhwa-Dong, Joong-Gu, Daejeon 301131, Republic of Korea.
Lee
Su Yel
SY
Lee
Mi Nam
MN
Kim
Jang-Hyun
JH
Chang
Gyu Tae
GT
Seok
Jeong Ho
JH
Lee
Choong Jae
CJ
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
D007529:Isoflavones; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D004815:Epidermal Growth Factor; C004742:daidzein; D000255:Adenosine Triphosphate