Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion.
We have recently shown that Src induces the formation of podosomes and cell invasion by suppressing endogenous p53, while enhanced p53 strongly represses the Src-induced invasive phenotype. However, the mechanism by which Src and p53 play antagonistic roles in cell invasion is unknown. Here we show that the Stat3 oncogene is a required downstream effector of Src in inducing podosome structures and related invasive phenotypes. Stat3 promotes Src phenotypes through the suppression of p53 and the p53-inducible protein caldesmon, a known podosome antagonist. In contrast, enhanced p53 attenuates Stat3 function and Src-induced podosome formation by upregulating the tumor suppressor PTEN. PTEN, through the inactivation of Src/Stat3 function, also stabilizes the podosome-antagonizing p53/caldesmon axis, thereby further enhancing the anti-invasive potential of the cell. Furthermore, the protein phosphatase activity of PTEN plays a major role in the negative regulation of the Src/Stat3 pathway and represses podosome formation. Our data suggest that cellular invasiveness is dependent on the balance between two opposing forces: the proinvasive oncogenes Src-Stat3 and the anti-invasive tumor suppressors p53-PTEN.
Mukhopadhyay
Utpal K
UK
Department of Biochemistry, Queen's University, Kingston, ON, Canada.
Mooney
Patrick
P
Jia
Lilly
L
Eves
Robert
R
Raptis
Leda
L
Mak
Alan S
AS
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002148:Calmodulin-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D055785:Gene Knockdown Techniques; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000502:physiology; D053506:Matrix Metalloproteinase 10 / Q000235:genetics / Q000502:physiology; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008954:Models, Biological; D050505:Mutant Proteins / Q000235:genetics / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000502:physiology; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology*; D019061:src-Family Kinases / Q000235:genetics / Q000502:physiology*
D002148:Calmodulin-Binding Proteins; D017931:DNA Primers; D061965:Matrix Metalloproteinase Inhibitors; D050505:Mutant Proteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D016159:Tumor Suppressor Protein p53; D019061:src-Family Kinases; D051059:PTEN Phosphohydrolase; D053506:Matrix Metalloproteinase 10; D020781:Matrix Metalloproteinase 1