Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production.
Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro 13C-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-13C-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched 13C-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors' knowledge this is the first report of highly enriched 13C-carotenoid production from in vitro plant cell culture.
Engelmann
Nancy J
NJ
Division of Nutritional Sciences, 905 South Goodwin Avenue, University of Illinois-Urbana/Champaign, Urbana, Illinois 61801, USA.
Campbell
Jessica K
JK
Rogers
Randy B
RB
Rupassara
S Indumathie
SI
Garlick
Peter J
PJ
Lila
Mary Ann
MA
Erdman
John W
JW
D002247:Carbon Isotopes; D002338:Carotenoids / Q000096:biosynthesis*; D002460:Cell Line; D007553:Isotope Labeling / Q000379:methods*; D018551:Solanum lycopersicum / Q000166:cytology / Q000378:metabolism*
D002247:Carbon Isotopes; D002338:Carotenoids