[Effect of magnesium isoglycyrrhizinate on allergy contact dermatitis (ACD) in mice].
OBJECTIVE
To investigate the effect of magnesium isoglycyrrhizinate (MgIG) on allergy contact dermatitis (ACD) in mice.


METHODS
The model of ACD was sensitized and challenged by 1% dinitrofluorobenzene(DNFB).48 SPF grade mice were divided into 6 groups randomly: a control group, a model group, three dosage groups and a positive group. The drug was injected through vena caudalis. The change of ear's swelling and the scores of ear's thickness and erythema of each mouse was observed. The level of INF-gamma, IgE, IL-4 in serum was detected by ELISA method. Then the pathologic change of mice ears was using HE staining examined under light microscope.


RESULTS
MgIG could decrease (P < 0.05) the ear's swelling, the scores of ear's thickness and erythema, and INF-gamma and IgE level in mice serum. It was observed that MgIG could significantly alleviate the infiltrate of inflam cell and the hemangiectasis in ear tissue.


CONCLUSIONS
Certain concentration of MgIG has significant therapeutic effect on ACD in mice. Therapeutic mechanism of MgIG may be relevant with the suppression of INF-gamma and IgE.
Xu
Jianguo
J
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. chxdejia@hotmail.com
Liu
Jiyong
J
Peng
Cheng
C
Liu
Yaming
Y
Hu
Jinhang
J
D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011897:Random Allocation; D012503:Saponins / Q000008:administration & dosage*; D014315:Triterpenes / Q000008:administration & dosage*
C521270:18alpha,20beta-hydroxy-11-oxo-norolean-12-en-3beta-yl-2-O-beta-D-glucopyranurosyl-alpha-D-glucopyranosiduronate magnesium tetrahydrate; D012503:Saponins; D014315:Triterpenes; D015847:Interleukin-4