Effect of oxalate desensitizers and dentin moisture during total-etch bonding.
OBJECTIVE
To evaluate the effect of oxalate during total-etch bonding, under different dentin moisture conditions, over time. The null hypothesis tested was that microtensile bond strength (microTBS) was not affected by oxalate treatment and dentin moisture during two evaluation periods.


METHODS
Extracted human third molars had their mid-coronal dentin exposed flat and polished with 600-grit SiC paper. The surfaces were etched with 35% phosphoric acid for 15 seconds, washed and blot dried. After etching, a 3% potassium oxalate gel was applied for 120 seconds, except for the control group (no desensitizer). The surface was then washed and left moist (Wet bonding) or air-dried for 30 seconds (Dry bonding). The surfaces were bonded with: (1) two 2-step etch-and-rinse adhesives: Single Bond (SB); Prime & Bond NT (PBNT) and (2) one 3-step etch-and-rinse adhesive: Scotchbond Multi Purpose (SBMP). Composite buildups were constructed incrementally with Tetric Ceram resin composite. Each increment was cured for 40 seconds. After storage in water for 24 hours or 1 year at 37 degrees C, the specimens were prepared for microTBS testing with a cross-sectional area of approximately 1 mm2. They were then tested in tension in an Instron machine at 0.5 mm/minute. Data were analyzed by ANOVA and Student-Newman-Keuls at alpha = 0.05.


RESULTS
Application of potassium oxalate had no significant effect on the bond strengths of SBMP and PBNT, regardless of the surface moisture condition (P > 0.05). Conversely, reduced bond strengths were observed after oxalate treatment for SB in both moisture conditions, that being significantly lower when using a dry-bonding procedure (P < 0.05). Lower bond strength was obtained for PBNT when a dry-bonding technique was used, regardless of the oxalate treatment (P < 0.05). After aging the specimens for 1 year, bond strengths decreased. Smaller reductions were observed for SBMP, regardless of moisture conditions. For the WB technique, smaller reductions after 1 year were observed without oxalate treatment for SB and after oxalate treatment for PBNT.
Almeida
Júlio C F
JC
Division of Dentistry, University Hospital of Brasilia, University of Brasilia-UnB, Brazil.
Osorio
Raquel
R
Garcia
Fernanda C P
FC
Osorio
Estrella
E
Carvalho
Ricardo M
RM
Toledano
Manuel
M
D000134:Acid Etching, Dental / Q000379:methods; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D001840:Dental Bonding / Q000379:methods*; D003799:Dental Stress Analysis; D003804:Dentin; D057133:Dentin Desensitizing Agents; D017220:Dentin-Bonding Agents; D006801:Humans; D008422:Materials Testing; D010070:Oxalates; D011109:Polymethacrylic Acids; D019279:Resin Cements; D013718:Tensile Strength; D014867:Water
D003188:Composite Resins; D057133:Dentin Desensitizing Agents; D017220:Dentin-Bonding Agents; D010070:Oxalates; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; C115612:single bond; D014867:Water; D017438:Bisphenol A-Glycidyl Methacrylate