2-deoxyribose deprives cultured astrocytes of their glutathione.
High concentrations of 2-deoxy-D-ribose (2dRib) have been reported to cause oxidative stress and to disturb the glutathione (GSH) metabolism of various cell types. Exposure of astrocyte-rich primary cultures to millimolar concentrations of 2dRib or its stereoisomer 2-deoxy-L-ribose, but not the incubation with ribose, 2-deoxyglucose, glucose, fructose or saccharose, lowered the cellular GSH content in a time and concentration dependent manner. After exposure for 4 h to 30 mM 2dRib the cells contained 2dRib in a concentration of about 24 mM. Under these conditions 2dRib did not compromise cell viability and the ability of the cells to synthesise GSH, nor were the cellular ratio of glutathione disulfide (GSSG) to GSH and the extracellular concentrations of GSH or GSSG increased. These data demonstrate that 2dRib deprives viable cultured astrocytes of GSH and suggest that a cellular reaction of GSH with 2dRib or its metabolites is involved in the deprivation of astrocytic GSH.
Schmidt
Maike M
MM
Center for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany.
Greb
Helena
H
Koliwer-Brandl
Hendrik
H
Kelm
Soerge
S
Dringen
Ralf
R
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003855:Deoxyribose / Q000494:pharmacology*; D005978:Glutathione / Q000096:biosynthesis / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D051381:Rats; D013237:Stereoisomerism
D003855:Deoxyribose; D005978:Glutathione; D019803:Glutathione Disulfide