Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of interferon-gamma.
There are multiple mechanisms that protect the intestine from an excessive inflammatory response to intestinal microorganisms. We report here that all four mammalian peptidoglycan recognition proteins (PGRPs or Pglyrps) protect the host from colitis induced by dextran sulfate sodium (DSS). Pglyrp1(-/-), Pglyrp2(-/-), Pglyrp3(-/-), and Pglyrp4(-/-) mice are all more sensitive than wild-type mice to DSS-induced colitis due to a more inflammatory gut microflora, higher production of interferon-gamma, higher expression of interferon-inducible genes, and an increased number of NK cells in the colon upon initial exposure to DSS, which leads to severe hyperplasia of the lamina propria, loss of epithelial cells, and ulceration in the colon. Thus, during experimental colitis, PGRPs protect the colon of wild-type mice from an early inflammatory response and the loss of the barrier function of intestinal epithelium by promoting normal bacterial flora and by preventing damaging production of interferon-gamma by NK cells in response to injury.
Saha
Sukumar
S
Indiana University School of Medicine Northwest, Gary, IN 46408, USA.
Jing
Xuefang
X
Park
Shin Yong
SY
Wang
Shiyong
S
Li
Xinna
X
Gupta
Dipika
D
Dziarski
Roman
R
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D003092:Colitis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D003106:Colon; D016264:Dextran Sulfate / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease; D007371:Interferon-gamma / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout
D002352:Carrier Proteins; C118897:peptidoglycan recognition protein; D007371:Interferon-gamma; D016264:Dextran Sulfate