Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
Ror receptor-tyrosine kinases act as Wnt-5a receptors in beta-catenin independent Wnt-signaling pathways. In Xenopus, expression of xPAPC is regulated by a Wnt-5a/Ror2 pathway, which resembles typical signaling cascades downstream of receptor-tyrosine kinases. Here, we have identified the phospho-tyrosine binding protein ShcA as an intracellular binding partner of Ror2. ShcA binds to a conserved motif in Ror2 via its SH2-domain. Wnt-5a induces clustering of Ror2 in the cell membrane and recruitment of ShcA to the Ror2 receptor complex. We further show that ShcA is co-expressed with Ror2 in developing Xenopus embryos and ShcA is required for Wnt-5a/Ror2 mediated upregulation of xPAPC, demonstrating the functional relevance of this interaction.
Feike
Ann Caroline
AC
University Erlangen-Nuremberg, Biology Dept., Developmental Biology Unit, Staudtstr. 5, D-91058 Erlangen, Germany.
Rachor
Klara
K
Gentzel
Marc
M
Schambony
Alexandra
A
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D051379:Mice; D000091363:Protocadherins; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein; D029867:Xenopus Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism; D018909:src Homology Domains
D015820:Cadherins; D002352:Carrier Proteins; C117635:Pcdh8 protein, Xenopus; D000091363:Protocadherins; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C085096:Wnt5a protein, Xenopus; D029867:Xenopus Proteins; D057050:Receptor Tyrosine Kinase-like Orphan Receptors