Eco1 is important for DNA damage repair in S. cerevisiae.
The cohesin network has an essential role in chromosome segregation, but also plays a role in DNA damage repair. Eco1 is an acetyltransferase that targets subunits of the cohesin complex and is involved in both the chromosome segregation and DNA damage repair roles of the network. Using budding yeast as a model system, we find that mutations in Eco1, including a genocopy of a human Roberts syndrome allele, do not cause gross defects in chromosome cohesion. We examined how mitotic and meiotic DNA damage repair is affected by mutations in Eco1. Strains containing mutations in Eco1 are sensitive to DNA damaging agents that cause double-strand breaks, such as X-rays and bleomycin. While meiotic crossing over is relatively unaffected in strains containing the Roberts mutation, reciprocal mitotic crossovers occur with extremely low frequency in this mutant background. Our results suggest that Eco1 promotes the reciprocal exchange of chromosome arms and maintenance of heterozygosity during mitosis.
Lu
Shuai
S
Stowers Institute for Medical Research, Kansas City, MO, USA.
Goering
Matthew
M
Gard
Scarlett
S
Xiong
Bo
B
McNairn
Adrian J
AJ
Jaspersen
Sue L
SL
Gerton
Jennifer L
JL
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000903:Antibiotics, Antineoplastic / Q000737:chemistry; D001761:Bleomycin / Q000737:chemistry; D018797:Cell Cycle Proteins / Q000378:metabolism; D064447:Checkpoint Kinase 2; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D008938:Mitosis; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014965:X-Rays; D000097722:Cohesins
D000123:Acetyltransferases; D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D018797:Cell Cycle Proteins; D064447:Checkpoint Kinase 2; D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C118076:ECO1 protein, S cerevisiae; C067022:RAD53 protein, S cerevisiae