Peptides from water buffalo cheese whey induced senescence cell death via ceramide secretion in human colon adenocarcinoma cell line.
METHODS
Milk proteins are a source of bioactive peptides. Recent studies have indicated that protein-derived peptides released in buffalo cheese acid whey exert a cytomodulatory effect in human epithelial colon cancer (CaCo2) cells. The aim of the present study was to explain the molecular mechanism involved in the response of CaCo2 cells to oxidative stress in the presence of peptide fractions of buffalo cheese whey, purified and characterized by mass spectrometry.


RESULTS
We demonstrated that treatment of CaCo2 treated with H2O2 (H-CaCo2) cells with a partially purified peptide sub-fraction (f3) from buffalo cheese acid whey induced a reduction of mitochondrial superoxide anion with subsequent decrease in heat shock protein 70 and 90 expression. Moreover, we observed a 5-fold decrease in cyclin A expression and cell cycle arrest in G1/G0 phases. These responses were associated with increased activity of alkaline phosphatase and beta-galactosidase, markers of differentiation and senescence respectively.


CONCLUSIONS
The structural characterization of the active peptide fraction and the elucidation of the effects induced by its treatment on H-CaCo2 cells in vitro demonstrated an activity of this peptide sub-fraction in the modulation of cell cycle, thus suggesting potential application for the development of nutraceuticals as well as health-promoting functional foods.
De Simone
Carmela
C
Department of Food Science, University of Naples Federico II, Portici, Naples, Italy.
Ferranti
Pasquale
P
Picariello
Gianluca
G
Scognamiglio
Ilaria
I
Dicitore
Alessandra
A
Addeo
Francesco
F
Chianese
Lina
L
Stiuso
Paola
P
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002020:Buffaloes; D018938:Caco-2 Cells; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002518:Ceramides / Q000378:metabolism*; D019925:Cyclin A / Q000378:metabolism; D019587:Dietary Supplements; D005260:Female; D005525:Food-Processing Industry / Q000191:economics; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007220:Industrial Waste / Q000191:economics; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000067816:Whey Proteins
D015415:Biomarkers; D002518:Ceramides; D019925:Cyclin A; D006360:Heat-Shock Proteins; D007220:Industrial Waste; D008894:Milk Proteins; D016877:Oxidants; D010455:Peptides; D020011:Protective Agents; D000067816:Whey Proteins