Changes in heart rate patterns by lipopolysaccharide and intermittent hypoxia-ischemia in 7-day-old rats.
OBJECTIVE
We previously reported that lipopolysaccharide (LPS) and hypoxia-ischemia (HI) act additively to induce brain damage in the developing rat model. The present study was undertaken to determine whether LPS-HI-induced brain damage is associated with changes in heart rate (HR) patterns.


METHODS
Seven-day-old Wistar rats were administered LPS (1 mg/kg, n = 17) or saline (n = 15) intraperitoneally. After 4 h, the left common carotid artery was ligated and electrocardiogram electrodes were placed on the chest under ether anesthesia, followed by intermittent HI (8% oxygen for 6 min) at 10-min intervals for a total of 10 times. Seven days later, rats were sacrificed and brains removed for histological examination. Neuronal damage for a single section was categorized as mild (≤ 25% of the surface area), moderate (25-50%) or severe (≥ 50%).


RESULTS
Brain damage was induced only in the LPS/HI group, which was statistically significant when compared to the saline/HI group. Baseline HR increased significantly due to LPS administration (P < 0.05). In the LPS/HI group, the amplitude of hypoxia-driven tachycardia decreased significantly in the last 5 hypoxic episodes in brain-damaged rats compared to rats with no visible damage (28 ± 1 vs 16 ± 2 bpm). Baseline HR variability was also suppressed significantly during the last five hypoxic episodes in brain-damaged rats compared to rats with no visible damage.


CONCLUSIONS
LPS administration caused a gradual decrease in baseline HR variability and blunted tachycardia in response to repetitive HI, suggesting these signs are indicative of future neonatal brain damage.
Yang
Li
L
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Miyazaki, Japan.
Sameshima
Hiroshi
H
Ikenoue
Tsuyomu
T
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects / Q000473:pathology; D006339:Heart Rate / Q000187:drug effects*; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides