title: DNA as a target for anticancer compounds screening directly by resonance light scattering technique.
abstract: Anticancer drugs which selectively interact with DNA can change DNA's conformation and inhibit the duplication or transcription of DNA. An instrument-based assay for directly screening DNA-targeted anticancer drugs using resonance light scattering (RLS) technique with a common spectrofluorometer was proposed. To monitor the proposed screening method, the interactions between three anticarcinogens (Adriamycin (ADM), Bleomycin A (BLMA), Actinomycin D (ACTD)) and DNA were studied. The sequence of binding constants for the three anticarcinogens obtained from RLS spectra is: K(RLS) (ACTD, 9.43 × 10(5) L mol(-1)) > K(RLS) (ADM, 6.67 × 10(5) L mol(-1)) > K(RLS) (BLMA, 8.88 × 10(3) L mol(-1)) and of binding numbers is: N(RLS) (ACTD, 3.36 mmol g(-1)) < N(RLS) (ADM, 3.81 mmol g(-1)) < N(RLS) (BLMA, 57.44 mmol g(-1)). From the results we got the sequence of combination intensity between these three drugs and DNA as follows: ACTD-DNA > ADM-DNA > BLMA-DNA, which was completely consistent with drug activity. The conclusion indicated that the present method was direct, rapid, reliable and was another important innovation of the application of RLS technique.
authors: Department of Chemistry, Shantou University, Shantou, 515063, China. kqlu@stu.edu.cn
authors: Zhanguang
authors: Z
authors: Chen
authors: Yurui
authors: Y
authors: Peng
authors: Maohuai
authors: M
authors: Chen
authors: Xi
authors: X
authors: Chen
authors: Guomin
authors: G
authors: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000737:chemistry*; D001761:Bleomycin / Q000097:blood / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003609:Dactinomycin / Q000097:blood / Q000737:chemistry; D004317:Doxorubicin / Q000097:blood / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D001761:Bleomycin; D003609:Dactinomycin; D004317:Doxorubicin; D004247:DNA