IL-17 contributes to autoimmune hepatitis.
The role of interleukin-17 (IL-17) in autoimmune hepatitis (AIH) was investigated. A mouse model of experimental autoimmune hepatitis was established, and the syngeneic S-100 antigen emulsified in complete Freud's adjuvant was injected intraperitoneally into adult male C57BL/6 mice. The IL-17 expression in serum and the livers of the mice models was detected by using ELISA and immunohistochemistry, respectively. IL-17 neutralizing antibody was used to study the biological effect of IL-17 in the experimental AIH. IL-17 neutralizing antibody in vivo administration alleviated the hepatic inflammation and ALT level in the AIH model. IL-17 in the peripheral blood mononuclear cells (PBMC) of AIH patients was measured by using real-time PCR method. The results showed that IL-17 level was significantly up-regulated in AIH patients and mice models. It was concluded that IL-17 contributed to the development of AIH and might be a potential therapeutic target of AIH.
Yu
Haijing
H
Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. yhjmarry@hotmail.com
Huang
Jiaquan
J
Liu
Yang
Y
Ai
Guo
G
Yan
Weiming
W
Wang
Xiaojing
X
Ning
Qin
Q
D000818:Animals; D005260:Female; D005620:Freund's Adjuvant; D019693:Hepatitis, Autoimmune / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D020381:Interleukin-17 / Q000097:blood* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009418:S100 Proteins; D015854:Up-Regulation
D020381:Interleukin-17; D009418:S100 Proteins; D005620:Freund's Adjuvant