title: Differential distributions of CSE1L/CAS and E-cadherin in the polarized and non-polarized epithelial glands of neoplastic colorectal epithelium.
abstract: Colorectal glands are important functional organs in colorectal tissue and are also the origin of colorectal carcinomas. Epithelial cell polarization of colorectal glands is related to structural integrity and physiological functions of colorectal glands as well as colorectal carcinoma formation. The cellular apoptosis susceptibility (CSE1L/CAS) protein has been shown to induce polarity formation of human colorectal cells in cell culture. E-cadherin expression in epithelial cells is crucial for the establishment and maintenance of epithelial cell polarity. In this study we examined the distributions of CSE1L and E-cadherin in the epithelial glands of normal and neoplastic colorectal epithelium and correlated these to polarity formation in the colorectal glands. Our results showed that CSE1L was differentially stained in the epithelial glands of neoplastic colorectal epithelium, and the staining was related to gland epithelial cell polarization and E-cadherin distribution. CSE1L was associated E-cadherin in GST pull-down experiments and immunoprecipitation assays. Basolateral staining of CSE1L and E-cadherin were seen in the polarized glands of normal and neoplastic colorectal epithelium. Absence of basolateral CSE1L staining in neoplastic epithelium glands was associated with loss of gland epithelial cell polarity, and this was parallel with E-cadherin staining. The non-polarized areas in epithelium glands showed a patchy staining for CSE1L and E-cadherin. These results indicate that examination of CSE1L and E-cadherin distribution in colorectal epithelium glands may be valuable for evaluating the malignance of colorectal disease.
authors: Division of Hematology and Oncology, Department of Internal Medicine, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
authors: Wu-Ching
authors: WC
authors: Uen
authors: Cheng-Jeng
authors: CJ
authors: Tai
authors: Shing-Chuan
authors: SC
authors: Shen
authors: Woan-Ruoh
authors: WR
authors: Lee
authors: Tang-Yi
authors: TY
authors: Tsao
authors: Win-Ping
authors: WP
authors: Deng
authors: Hung-Yi
authors: HY
authors: Chiou
authors: Chung-Huei
authors: CH
authors: Hsu
authors: Cheng-I
authors: CI
authors: Hsieh
authors: Ching-Fong
authors: CF
authors: Liao
authors: Ming-Chung
authors: MC
authors: Jiang
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D016764:Cell Polarity; D029563:Cellular Apoptosis Susceptibility Protein / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology*; D011485:Protein Binding
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D029563:Cellular Apoptosis Susceptibility Protein