Serum gastrin level and pepsinogen I/II ratio as biomarker of Helicobacter pylori chronic gastritis.
OBJECTIVE
to find out biomarker as diagnostic tool of H. pylori chronic gastritis.


METHODS
the design of present study was a diagnostic test and there were 104 subjects with H. pylori chronic gastritis who fulfilled the inclusion and exclusion criteria. The diagnosis of H. pylori chronic gastritis was based on histopathological examination and PCR with ureC primer of the gastric biopsy specimen. In addition, we also performed the examination of serum gastrin, pepsinogen (PG) I and PG (pepsinogen) II level. By using analysis of receiver operating characteristic (ROC), an optimal cut off point of serum gastrin, PGI and PGII level as well as PGI/PGII ratio was determined. Analysis of bivariate logistic regression was used to determine the involved independent variables and possibilities as biomarkers. Significance level was determined by p value <0.05.


RESULTS
we found optimal cut off points on serum gastrin, PGI and PGII level as well as the PGI/PGII ratio at 5.89 pmol/L; 82.5 µg/L; 6.48 µg/L and 13.6 respectively. By using the analysis of bivariate logistic regression, we found gastrin level with p = 0.078 (OR 2.75;95%CI 0.89-8.45) and PGI/PGII ratio with p = 0.000 (OR 14.63;95%CI 3.55-60.63). The opportunity of gastrin level and PGI/PGII ratio as biomarkers was 0.8 with 47% sensitivity, 83% specificity, 74% PPV, 61% NPV, 65% accuracy, LR+ = 2.76 and LR- = 0.64.


CONCLUSIONS
gastrin level of >5.89 pmol/L and PGI/PGII ratio ≤13.6 can be utilized as biomarkers of H. pylori chronic gastritis.
Arinton
I Gede
IG
Department of Internal Medicine, Faculty of Medicine and Health, General Sudirman University - Margono Regional Public Hospital, Jl. Dr. Gumbreg 1, Purwokerto, Indonesia. gedearinton@yahoo.com
D001706:Biopsy; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D005755:Gastrins / Q000097:blood*; D005756:Gastritis / Q000097:blood* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000097:blood / Q000150:complications* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D020116:Pepsinogen A / Q000097:blood*; D020115:Pepsinogen C / Q000097:blood*; D016133:Polymerase Chain Reaction; D012372:ROC Curve
D004269:DNA, Bacterial; D005755:Gastrins; D020115:Pepsinogen C; D020116:Pepsinogen A