title: [Biological characteristics of T-lineage acute lymphoblastic leukemia in 23 children].
abstract: OBJECTIVE
  To investigate the biological characteristics of childhood T-lineage acute lymphoblastic leukemia (T-ALL) and their clinical significance.
  
  
  METHODS
  Immunophenotyping was performed by three-color flow cytometry analysis using CD45 /SSC gating in 23 children with newly diagnosed T-ALL. Meanwhile cytogenetic analysis was performed.
  
  
  RESULTS
  CD3(+) expression of T-lineage antigens was apparently higher than CD7(+) and CD5(+) expression. CD19(+) expression of B-lineage antigens was apparently higher than CD22(+), CD10(+) and CD20(+) expression. Myeloid antigen was expressed in 4 cases (17%). CD34(+) and HLA-DR(+) were observed in 4 cases (17%) and 5 cases (22%), respectively. cCD3(+) and cCD79(+) were expressed in 23 cases (100%) and 22 cases (96%), respectively. The chromosome detection in 8 cases with T-ALL showed hyperdiploid or Ph(+) chromosome (one case each). The fusion gene detection in 5 cases showed MLL rearrangements in two cases and positive SIL/TAL1 fusion gene in one case. CD3 expression was related with the complete remission rate.
  
  
  CONCLUSIONS
  Immunophenotyping is an important tool for diagnosis of T-ALL. However, the immunophenotype of T-ALL is heterogeneous. So, immunophenotyping along with cytogenetic and molecular genetic analysis is needed in the treatment and prognosis evaluation of T-ALL.
authors: Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong Technology and Science University, Wuhan 430030, China.
authors: Hao
authors: H
authors: Xiong
authors: Yao-Dong
authors: YD
authors: Zhang
authors: Qun
authors: Q
authors: Hu
authors: Yan
authors: Y
authors: Sun
authors: Shuang-You
authors: SY
authors: Liu
authors: Liu-Qing
authors: LQ
authors: Zhang
authors: Ai-Guo
authors: AG
authors: Liu
authors: Guan-Ling
authors: GL
authors: Wang
mesh_terms: D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*