Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.
A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.
Tanomsing
Naowarat
N
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Imwong
Mallika
M
Theppabutr
Sasikrit
S
Pukrittayakamee
Sasithon
S
Day
Nicholas P J
NP
White
Nicholas J
NJ
Snounou
Georges
G
D021901:DNA, Intergenic; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods*; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D013940:Thymidylate Synthase / Q000235:genetics*
D021901:DNA, Intergenic; D016054:DNA, Protozoan; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase