Microcarrier bioreactor culture system promotes propagation of human intervertebral disc cells.
BACKGROUND
Cell-based tissue engineering has emerged as a potential therapy for intervertebral disc degeneration. However, propagating and maintaining high quantity and quality of the seed cells remains a challenge.


OBJECTIVE
To investigate the feasibility of culturing human disc cells using a microcarrier bioreactor system.


METHODS
Cell counts, growth patterns, cell cycles and cellular viability were examined during the course of cell cultivation and compared between the microcarrier bioreactor culture system and the conventional monolayer culture.


RESULTS
Cultures in the microcarrier bioreactor resulted in enhanced disc cell growth and satisfactory cell viability in comparison with the conventional monolayer culture. The cells in the microcarrier bioreactor cultivation exhibited higher S phase ratios, elevated mitotic index and persistent exponential growth.


CONCLUSIONS
The microcarrier bioreactor culture system appears suitable for human disc cell propagation and may provide considerably more seeding cells for the tissue engineering process of intervertebral discs.
Zhang
L
L
Orthopedic Department, Shandong University Affiliated Provincial Hospital, Jinan, China.
Ning
B
B
Jia
T
T
Gong
W
W
Cong
M
M
Chen
J-F
JF
Yang
S-Y
SY
D000328:Adult; D000368:Aged; D019149:Bioreactors; D018929:Cell Culture Techniques; D002453:Cell Cycle; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007403:Intervertebral Disc / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D023822:Tissue Engineering