title: The laboratory impact of changing syphilis screening from the rapid-plasma reagin to a treponemal enzyme immunoassay: a case-study from the Greater Toronto Area.
abstract: BACKGROUND
  In 2005, syphilis screening in the Greater Toronto Area of Canada moved from the rapid plasma reagin (RPR) to a treponemal enzyme immunoassay (EIA). We sought to understand the consequences of this change on laboratory results and testing patterns with a population-based retrospective study of laboratory-based diagnoses of syphilis.
  
  
  METHODS
  Samples positive under RPR (1998-2005) and EIA (2005-2008) screening were confirmed with an alternate treponemal test, and during the latter period underwent RPR testing. We compared monthly rates and the forecasting relationship between positives and future submissions with time-series methods, and assessed risk factors for EIA(+)/RPR(-) results using Poisson regression.
  
  
  RESULTS
  A total of 3,092,938 submissions were included. Following EIA implementation, confirmed positive rates increased by 10.3 per 100,000 population (P<0.001). 0.59% of EIA(+)/RPR(-) individuals converted to RPR(+) within 2 months. EIA(+)/RPR(-) patients were more likely to be male (incidence rate ratio [IRR]: 2.3, 95% confidence interval [CI]: 1.6-2.5), asymptomatic (IRR: 1.8, 95% CI: 1.3-2.8), and aged>50 years (IRR: 2.4, 95% CI: 1.6-3.5) than those with EIA(+)/RPR(+) results. We detected a significant positive feedback loop between positive tests and subsequent submissions. This relationship was only transiently evident for EIA(+)/RPR(-) results up to 1 year following the changeover.
  
  
  CONCLUSIONS
  EIA screening facilitates identification of probable latent syphilis and earlier serological detection of infectious syphilis, but may transiently cause increases in testing and indirectly suggests that physicians' interpretation of RPR(-) serology may lead to partner testing. In the absence of a true gold standard, implementation of EIA screening warrants careful communication regarding serological interpretation.
authors: Division of Infectious Diseases, University of Toronto, and Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada. sharmistha.mishra@utoronto.ca
authors: Sharmistha
authors: S
authors: Mishra
authors: Marie-Claude
authors: MC
authors: Boily
authors: Victoria
authors: V
authors: Ng
authors: Wayne L
authors: WL
authors: Gold
authors: Tom
authors: T
authors: Okura
authors: Marsha
authors: M
authors: Shaw
authors: Tony
authors: T
authors: Mazzulli
authors: David N
authors: DN
authors: Fisman
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000706:statistics & numerical data; D015994:Incidence; D016014:Linear Models; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D009864:Ontario; D011935:Reagins / Q000097:blood*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014210:Treponema pallidum / Q000276:immunology / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D011935:Reagins