A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast.
Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2 degrees C and 50 degrees C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast.
Velve-Casquillas
Guilhem
G
Institut Curie, UMR 144 CNRS, Paris 75005, France.
Costa
Judite
J
Carlier-Grynkorn
Frédérique
F
Mayeux
Adeline
A
Tran
Phong T
PT
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D055616:Microtechnology / Q000379:methods; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D055503:Protein Multimerization / Q000502:physiology; D012568:Schizosaccharomyces / Q000737:chemistry / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D029702:Schizosaccharomyces pombe Proteins