Chronic resveratrol intake reverses pro-inflammatory cytokine profile and oxidative DNA damage in ageing hybrid mice.
Thymic involution and shrinkage of secondary lymphoid organs are leading causes of the deterioration of the T-cell compartment with age. Inflamm-aging, a sustained inflammatory status, has been associated with chronic diseases and shortened longevity. This is the first study to investigate the effect of treating aging hybrid mice with long-term, low-dose resveratrol (RSV) in drinking water by assessing multiple immunological markers and profiles in the immune system. We found that hybrid mice exhibited marked age-related changes in the CD3+CD4+, C3+CD8+, CD4+CD25+, CD4M and CD8M surface markers. RSV reversed surface phenotypes of old mice to that of young mice by maintaining the CD4+ and CD8+ population in splenocytes as well as reducing CD8+CD44+ (CD8M) cells in the aged. RSV also enhanced the CD4+CD25+ population in old mice. Interestingly, pro-inflammatory status in young mice was transiently elevated by RSV but it consequently mitigated the age-dependent increased pro-inflammatory cytokine profile while preserving the anti-inflammatory cytokine condition in the old mice. Age-dependent increase in 8OHdG, an oxidative DNA damage marker was ameliorated by RSV. Immunological-focused microarray gene expression analysis showed that only the CD72 gene was significantly downregulated in the 12-month RSV-treated mice compared to age-matched controls. Our study indicates that RSV even at low physiological relevant levels is able to affect the immune system without causing marked gene expression changes.
Wong
Yee Ting
YT
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore. ytwong@alumni.nus.edu.sg
Gruber
Jan
J
Jenner
Andrew M
AM
Tay
Francis Eng Hock
FE
Ruan
Runsheng
R
D000375:Aging / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D000975:Antioxidants / Q000494:pharmacology*; D002452:Cell Count; D016207:Cytokines / Q000276:immunology*; D004249:DNA Damage / Q000187:drug effects*; D007249:Inflammation / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D000077185:Resveratrol; D013154:Spleen / Q000276:immunology; D013267:Stilbenes / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000954:Antigens, Surface; D000975:Antioxidants; D016207:Cytokines; D013267:Stilbenes; D000077185:Resveratrol