title: Cathepsin D messenger RNA is downregulated in human lung cancer.
abstract: OBJECTIVE
  Lysosomal proteases cathepsins B and D (CB and CD) play a significant part in cancer progression. For many oncological diseases protein expression levels of CB and CD have been investigated and correlations with tumour characteristics revealed. Meanwhile, there is very little information concerning mRNA expression level.
  
  
  METHODS
  In the present work, data about mRNA levels of CB and CD in human lung cancer was obtained using reverse transcription followed by real-time polymerase chain reaction.
  
  
  RESULTS
  For the first time CD and CB mRNA in human lung cancer tumours was quantified. It was shown that CB and CD mRNA levels do not correlate with any tumour characteristics. However, in most analysed tumours, expression of CD mRNA was downregulated compared with adjacent normal tissue (p <0.0003).
  
  
  CONCLUSIONS
  The data obtained indicate CD mRNA as a potential lung cancer marker.
authors: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
authors: Andrey V
authors: AV
authors: Shubin
authors: Ilya V
authors: IV
authors: Demidyuk
authors: Alexander M
authors: AM
authors: Kurinov
authors: Vladimir V
authors: VV
authors: Demkin
authors: Tatyana V
authors: TV
authors: Vinogradova
authors: Marina V
authors: MV
authors: Zinovyeva
authors: Alexander V
authors: AV
authors: Sass
authors: Irina B
authors: IB
authors: Zborovskaya
authors: Sergey V
authors: SV
authors: Kostrov
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002402:Cathepsin D / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; D012333:RNA, Messenger; D002402:Cathepsin D