Fabrication and evaluation of chitosan-gelatin based buckling implant for retinal detachment surgery.
The traditional nonabsorbable silicone buckling implant (buckle) may cause some long-term complications when it is used in the retinal detachment surgery. In this study we fabricated a chitosan-gelatin based buckling implant to replace the traditional one. We evaluated its biocompatibility with human scleral fibroblasts (HSF) in vitro and its cytotoxicity with L929 cell. To evaluate elasticity and hardness of the blends buckle, the mechanical properties of the chitosan-gelatin buckle were compared with the traditional silicone buckle. The light and electron microscopy coupled with immunocytochemistry demonstrated that chitosan-gelatin blends supported the survival and growth of primarily cultured HSF without significant cytotoxic effects. MTT analysis and cell cycle analysis indicated that chitosan-gelatin blends promoted the proliferation of HSF. A preliminary in vivo implantation test indicated that chitosan-gelatin buckling implant were compatible with the surrounding tissue. The results collectively demonstrated that chitosan-gelatin blends could be a candidate biodegradable material for scleral buckling surgery.
Chen
Hui
H
Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, People's Republic of China.
Zhao
Zhi
Z
Zhao
Yahong
Y
Yang
Yumin
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000633:toxicity; D019245:Compressive Strength; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D011817:Rabbits; D012163:Retinal Detachment / Q000601:surgery*; D012590:Sclera / Q000166:cytology / Q000378:metabolism; D012591:Scleral Buckling / Q000379:methods*; D012828:Silicones; D017550:Spectroscopy, Fourier Transform Infrared
D001672:Biocompatible Materials; D012828:Silicones; D005780:Gelatin; D048271:Chitosan