title: [Synthesis and identification of artificial antigens for sulfaguanidine and sulfathiazole].
abstract: OBJECTIVE
  To prepare artificial antigens of sulfaguanidine and sulfathiazole for detecting sulfaguanidine and sulfathiazole in foods of animal origin.
  
  
  METHODS
  The artificial antigens were synthesized by the diazotization methods of coupling sulfaguanidine and sulfathiazole with bovine serum albumin (BSA), and the coupling ratio was 10 : 1 and 9 : 1 respectively. The anti-serum of Balb/c mice was obtained after 5 injections of artificial antigens and the antibody titles were detected by iELISA method.
  
  
  RESULTS
  The artificial antigens were identified by infrared spectra, ultraviolet spectra and immunized experimental animals.
  
  
  CONCLUSIONS
  The antigens for sulfaguanidine and sulfathiazole were acquired successfully. The results have laid basis for the specific immunoassay of sulfaguanidine and sulfathiazole.
authors: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. yhcui@hit.edu.cn
authors: Yanhua
authors: Y
authors: Cui
authors: Hailing
authors: H
authors: Zhang
authors: Ying
authors: Y
authors: ma
authors: Cuihong
authors: C
authors: Dai
authors: Jie
authors: J
authors: Cui
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D004350:Drug Residues / Q000032:analysis; D005260:Female; D005504:Food Analysis / Q000379:methods; D006241:Haptens / Q000737:chemistry; D007114:Immunization; D007118:Immunoassay / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013414:Sulfaguanidine / Q000032:analysis / Q000276:immunology*; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000032:analysis / Q000276:immunology*
chemical_list: D000941:Antigens; D006241:Haptens; D013432:Sulfathiazoles; D013414:Sulfaguanidine; D012710:Serum Albumin, Bovine; D000077589:Sulfathiazole