title: Gene based prediction of clinically localized prostate cancer progression after radical prostatectomy.
abstract: OBJECTIVE
  Accurate estimates of recurrence risk are needed for optimal treatment of patients with clinically localized prostate cancer. We combined an established nomogram and what to our knowledge are novel molecular predictors into a new prognostic model of prostate specific antigen recurrence.
  
  
  METHODS
  We analyzed gene expression profiles from formalin fixed, paraffin embedded, localized prostate cancer tissues to identify genes associated with prostate specific antigen recurrence. Profiles of the identified markers were reproduced by reverse transcriptase-polymerase chain reaction. We used the profiles of 3 of these genes along with output from the Kattan postoperative nomogram to produce a predictive model of prostate specific antigen recurrence.
  
  
  RESULTS
  After variable selection we built a model of prostate specific antigen recurrence combining expression values of 3 genes and the postoperative nomogram. The 3-gene plus nomogram model predicted 5-year prostate specific antigen recurrence with a concordance index of 0.77 in a validation set compared to a concordance index of 0.67 for the nomogram. This model identified a subgroup of patients at high risk for recurrence that was not identified by the nomogram.
  
  
  CONCLUSIONS
  This new gene based classifier has superior predictive power compared to that of the 5-year nomogram to assess the risk of prostate specific antigen recurrence in patients with organ confined prostate cancer. Our classifier should provide more accurate stratification of patients into high and low risk groups for treatment decisions and adjuvant clinical trials.
authors: Veridex LLC, Raritan, New Jersey, USA.
authors: Dmitri
authors: D
authors: Talantov
authors: Timothy A
authors: TA
authors: Jatkoe
authors: Maret
authors: M
authors: Böhm
authors: Yi
authors: Y
authors: Zhang
authors: Alison M
authors: AM
authors: Ferguson
authors: Phillip D
authors: PD
authors: Stricker
authors: Michael W
authors: MW
authors: Kattan
authors: Robert L
authors: RL
authors: Sutherland
authors: James G
authors: JG
authors: Kench
authors: Yixin
authors: Y
authors: Wang
authors: Susan M
authors: SM
authors: Henshall
mesh_terms: D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D009364:Neoplasm Recurrence, Local / Q000453:epidemiology / Q000235:genetics*; D049451:Nomograms; D011379:Prognosis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D018570:Risk Assessment