Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
In response to a variety of extracellular ligands, the STAT (signal transducer and activator of transcription) transcription factors are rapidly recruited from their latent state in the cytoplasm to cell surface receptors where they are activated by phosphorylation at a single tyrosine residue(1). They then dimerize and translocate to the nucleus to drive the transcription of target genes, affecting growth, differentiation, homeostasis and the immune response. Not surprisingly, given their widespread involvement in normal cell processes, dysregulation of STAT function contributes to human disease, particularly to cancers(2) and autoimmune diseases(3). It is well established that transcription is regulated by alterations to the chromatin template(4,5). These alterations include the activities of ATP-dependent complexes, as well as covalent histone modifications and DNA methylation(6). Because STAT activation of gene expression is both rapid and transient, it requires specific mechanisms for modulating the chromatin template at STAT-dependent gene loci. To define these mechanisms, we characterize the histone modifications and the enzymatic activities that generate them at gene loci that respond to STAT signaling. This protocol describes chromatin immunoprecipitation, a method that is valuable for the study of STAT signaling to chromatin in activated gene expression.
Buro
Lauren J
LJ
Department of Biology, University of Virginia, VA, USA.
Shah
Shaili
S
Henriksen
Melissa A
MA
D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D002843:Chromatin; D006657:Histones; D050791:STAT Transcription Factors