Glucocorticoid receptor gene haplotype structure and steroid therapy outcome in IBD patients.
OBJECTIVE
To study whether the glucocorticoid receptor (GR/NR3C1) gene haplotypes influence the steroid therapy outcome in inflammatory bowel disease (IBD).


METHODS
We sequenced all coding exons and flanking intronic sequences of the NR3C1 gene in 181 IBD patients, determined the single nucleotide polymorphisms, and predicted the NR3C1 haplotypes. Furthermore, we investigated whether certain NR3C1 haplotypes are significantly associated with steroid therapy outcomes.


RESULTS
We detected 13 NR3C1 variants, which led to the formation of 17 different haplotypes with a certainty of > 95% in 173 individuals. The three most commonly occurring haplotypes were included in the association analysis of the influence of haplotype on steroid therapy outcome or IBD activity. None of the NR3C1 haplotypes showed statistically significant association with glucocorticoid therapy success.


CONCLUSIONS
NR3C1 haplotypes are not related to steroid therapy outcome.
Mwinyi
Jessica
J
Division of Clinical Pharmacology and Toxicology, University Hospital, Zurich, Switzerland.
Wenger
Christa
C
Eloranta
Jyrki J
JJ
Kullak-Ublick
Gerd A
GA
D000328:Adult; D016009:Chi-Square Distribution; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000235:genetics*; D003424:Crohn Disease / Q000188:drug therapy* / Q000235:genetics*; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007438:Introns; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D013256:Steroids / Q000627:therapeutic use*; D013557:Switzerland; D016896:Treatment Outcome; D055815:Young Adult
C497713:NR3C1 protein, human; D011965:Receptors, Glucocorticoid; D013256:Steroids