An antibiotic selection marker for nematode transgenesis.
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
Giordano-Santini
Rosina
R
Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France.
Milstein
Stuart
S
Svrzikapa
Nenad
N
Tu
Domena
D
Johnsen
Robert
R
Baillie
David
D
Vidal
Marc
M
Dupuy
Denis
D
D000818:Animals; D030801:Animals, Genetically Modified; D002107:Caenorhabditis / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D004351:Drug Resistance / Q000187:drug effects / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology*; D009355:Neomycin / Q000494:pharmacology; D012641:Selection, Genetic / Q000187:drug effects* / Q000235:genetics*; D019076:Transgenes / Q000235:genetics*
D005819:Genetic Markers; D005839:Gentamicins; C010680:antibiotic G 418; D009355:Neomycin