title: Expression and localization of angiogenic growth factors in developing porcine mesonephric glomeruli.
abstract: The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations.
authors: Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Ward.DeSpiegelaere@UGent.be
authors: Ward
authors: W
authors: De Spiegelaere
authors: Pieter
authors: P
authors: Cornillie
authors: Tim
authors: T
authors: Erkens
authors: Denis
authors: D
authors: Van Loo
authors: Christophe
authors: C
authors: Casteleyn
authors: Mario
authors: M
authors: Van Poucke
authors: Christian
authors: C
authors: Burvenich
authors: Luc
authors: L
authors: Van Hoorebeke
authors: Chris
authors: C
authors: Van Ginneken
authors: Luc
authors: L
authors: Peelman
authors: Wim
authors: W
authors: Van den Broeck
mesh_terms: D042683:Angiopoietin-1 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D042702:Angiopoietin-2 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000196:embryology* / Q000378:metabolism*; D008650:Mesonephros / Q000196:embryology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2