title: Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells.
abstract: Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure-function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high acrylate content, have a moderate wettability and employ integrin alpha(v)beta(3) and alpha(v)beta(5) engagement with adsorbed vitronectin to promote colony formation. The structure-function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors: Ying
authors: Y
authors: Mei
authors: Krishanu
authors: K
authors: Saha
authors: Said R
authors: SR
authors: Bogatyrev
authors: Jing
authors: J
authors: Yang
authors: Andrew L
authors: AL
authors: Hook
authors: Z Ilke
authors: ZI
authors: Kalcioglu
authors: Seung-Woo
authors: SW
authors: Cho
authors: Maisam
authors: M
authors: Mitalipova
authors: Neena
authors: N
authors: Pyzocha
authors: Fredrick
authors: F
authors: Rojas
authors: Krystyn J
authors: KJ
authors: Van Vliet
authors: Martyn C
authors: MC
authors: Davies
authors: Morgan R
authors: MR
authors: Alexander
authors: Robert
authors: R
authors: Langer
authors: Rudolf
authors: R
authors: Jaenisch
authors: Daniel G
authors: DG
authors: Anderson
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials