[Optimization of method of titration rabies virus with fluorescence focus units assay].
OBJECTIVE
A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.


METHODS
The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.


RESULTS
Data generated indicateed that a significant correlation between the two methods (r=99).


CONCLUSIONS
This method is simple and rapid. It will be considered as a useful alternative method to the LD50.
Chai
Miao
M
Wuhan Institute of Biological Products, Wuhan 430060, Hubei, China.
Xu
Ge-lin
GL
Sun
Wen
W
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D051379:Mice; D011818:Rabies / Q000821:virology*; D011820:Rabies virus / Q000737:chemistry / Q000502:physiology*
D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate