Pancreatic islet culture and preservation strategies: advances, challenges, and future outlook.
Postisolation islet survival is a critical step for achieving successful and efficient islet transplantation. This involves the optimization of islet culture in order to prolong survival and functionality in vitro. Many studies have focused on different strategies to culture pancreatic islets in vitro through manipulation of culture media, surface modified substrates, and the use of various techniques such as encapsulation, embedding, scaffold, and bioreactor culture strategies. This review aims to present and discuss the different methodologies employed to optimize pancreatic islet culture in vitro as well as address their respective advantages and drawbacks.
Daoud
Jamal
J
Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, QC, Canada.
Rosenberg
Lawrence
L
Tabrizian
Maryam
M
D000818:Animals; D019149:Bioreactors; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D004339:Drug Compounding; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*; D054457:Tissue Scaffolds
D003470:Culture Media