title: Insulin resistance and oxidative stress influence colony-forming unit-endothelial cells capacity in obese patients.
abstract: The aim of this study was to investigate the relationship between a sub-population of endothelial progenitor cells (EPC), namely colony-forming unit-endothelial cells (CFU-EC), their colony-forming capacity and variable clinical parameters, including insulin resistance and oxidative stress, in obese individuals. Thirty-eight obese adults (aged 42.5 ± 12.7), with BMI 32.3 ± 4.0 and 13 normal-weight controls (aged 48.2 ± 12.9; BMI 23.2 ± 2.3) were studied. CFU-EC colony-forming capacity was impaired in the group of obese individuals compared to the normal-weight controls (P = 0.001). The inverse correlation between homeostasis model assessment-insulin resistance (HOMA(IR)) index and CFU-EC number (r = -0.558, P < 0.0001) as well as positive total antioxidant status of plasma (TAS)/CFU-EC relation were noticed during the study. Additionally, correlations between the concentration of triglycerides (TG), high-density lipoproteins (HDLs), and body composition parameters in the obese participants were established. Our results demonstrate that insulin resistance and oxidative stress have a significant impact on the CFU-EC colony formation in obesity. Moreover, in multivariate regression analysis, in both studied groups, the HOMA(IR) index and HDL concentration were independent predictors of the number of CFU-EC. Endothelium dysfunction, which can be present in obesity, may in part be caused by EPC function impairment in this condition.
authors: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland. emilka@ump.edu.pl
authors: Ewa
authors: E
authors: Miller-Kasprzak
authors: Pawet
authors: P
authors: Bogdański
authors: Danuta
authors: D
authors: Pupek-Musialik
authors: Pawet P
authors: PP
authors: Jagodziński
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001823:Body Composition; D015992:Body Mass Index; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000473:pathology*; D018384:Oxidative Stress; D012044:Regression Analysis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D014280:Triglycerides / Q000097:blood
chemical_list: D008075:Lipoproteins, HDL; D014280:Triglycerides