Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.
Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.
Hyun
Seok-Hee
SH
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA.
Kim
Hee-kwon
HK
Kim
Jong-Mok
JM
Thompson
David H
DH
D017103:Bacillus Phages; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry*; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D010742:Phospholipid Ethers / Q000378:metabolism*; D011487:Protein Conformation
C480343:2,2'-di-O-decyl-3,3'-O-1,20-eicosanyl-bis-rac-glycero-1,1'-diphosphocholine; D036022:Capsid Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D010742:Phospholipid Ethers; C097975:portal protein, bacteriophage phi29; D006639:Histidine