title: A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast.
abstract: Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2 degrees C and 50 degrees C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast.
authors: Institut Curie, UMR 144 CNRS, Paris 75005, France.
authors: Guilhem
authors: G
authors: Velve-Casquillas
authors: Judite
authors: J
authors: Costa
authors: Frédérique
authors: F
authors: Carlier-Grynkorn
authors: Adeline
authors: A
authors: Mayeux
authors: Phong T
authors: PT
authors: Tran
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D055616:Microtechnology / Q000379:methods; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D055503:Protein Multimerization / Q000502:physiology; D012568:Schizosaccharomyces / Q000737:chemistry / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D029702:Schizosaccharomyces pombe Proteins