Tylosin and chlortetracycline effects during swine manure digestion: influence of sodium azide.
The antibiotics tylosin and chlortetracycline (CTC), which are commonly used in pig production, were studied to determine their effects on swine manure digestion in the presence and absence of biocide sodium azide. CTC enhanced initial hydrolysis reactions through volatile suspended solids production, while inhibiting methane and carbon dioxide production. Tylosin did not affect methane and carbon dioxide production; however, the relative abundance of both hydrogen utilizing and acetate-only utilizing microbial populations was significantly compromised. Sodium azide in the absence of antibiotics enhanced metabolic output and initial biomass production, and this observation suggests that populations of Methanobacteriales and Methanosaetaceae spp. appeared to contain sufficient periplasmic bound reductase to effectively utilize acetate and hydrogen in the presence of sodium azide. However, the combination of sodium azide and either CTC or tylosin was a very effective metabolic inhibitor, inhibiting methane and carbon dioxide production and VSS consumption compared to their no-azide counterpart.
Stone
James J
JJ
Department of Civil and Environmental Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA. james.stone@sdsmt.edu
Clay
Sharon A
SA
Spellman
Garth M
GM
D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D002245:Carbon Dioxide / Q000032:analysis; D002751:Chlortetracycline / Q000494:pharmacology*; D008372:Manure / Q000032:analysis*; D008697:Methane / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D019810:Sodium Azide / Q000494:pharmacology*; D034421:Sus scrofa; D013997:Time Factors; D015645:Tylosin / Q000494:pharmacology*; D014835:Volatilization / Q000187:drug effects
D008372:Manure; D002245:Carbon Dioxide; D019810:Sodium Azide; D008697:Methane; D002751:Chlortetracycline; D015645:Tylosin