Kinetic analysis of RNA interference for lamin A/C in HeLa cells.
We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.
You
Sung-Ju
SJ
Department of Biomedical Engineering, Korea University, Jeongneung-dong, Seongbuk-gu, Seoul, Republic of Korea.
Lee
Sang-Hoon
SH
Lee
Jae-Seung
JS
D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D034904:Lamin Type A / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D034904:Lamin Type A; D034741:RNA, Small Interfering; C440308:lamin C