title: Strip-based amperometric detection of myeloperoxidase.
abstract: The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18 U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.
authors: Department of NanoEngineering, University of California-San Diego, La Jolla, CA 92093, United States.
authors: Joshua R
authors: JR
authors: Windmiller
authors: Soujanya
authors: S
authors: Chinnapareddy
authors: Padmanabhan
authors: P
authors: Santhosh
authors: Jan
authors: J
authors: Halámek
authors: Min-Chieh
authors: MC
authors: Chuang
authors: Vera
authors: V
authors: Bocharova
authors: Ta-Feng
authors: TF
authors: Tseng
authors: Tzu-Yang
authors: TY
authors: Chou
authors: Evgeny
authors: E
authors: Katz
authors: Joseph
authors: J
authors: Wang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009195:Peroxidase / Q000032:analysis*; D011934:Reagent Strips
chemical_list: D011934:Reagent Strips; D009195:Peroxidase