Use of gadolinium diethylene triamine penta-acetic acid, as measured by ELISA, in the determination of glomerular filtration rates in cats.
The goal of this study was to evaluate a commercially available assay for gadolinium diethylene triamine penta-acetic acid (Gd-DTPA) for use in estimating glomerular filtration rate (GFR) in cats (Gd-DTPA GFR) with a wide range of GFRs. Eighteen adult cats (11 healthy and seven with chronic kidney disease) were included. Plasma concentrations of Gd-DTPA following intravenous injection were measured with an ELISA kit (FIT-GFR). Results for Gd-DTPA GFR were compared with simultaneously obtained values for plasma clearance of iohexol (iohexol GFR), plasma blood urea nitrogen (BUN) and creatinine concentrations. A negative correlation existed between iohexol GFR and plasma concentrations of BUN and creatinine. A positive correlation existed between Gd-DTPA GFR and iohexol GFR. There was no correlation between Gd-DTPA GFR and plasma concentrations of BUN and creatinine. In this study plasma clearance of Gd-DTPA assayed by FIT-GFR did not appear to provide a sufficiently accurate estimation of GFR in cats when compared with plasma clearance of iohexol, and plasma concentrations of BUN and creatinine.
Sox
Erika M
EM
Cornell University Hospital for Animals, Companion Animal Hospital, Ithaca, NY 14853, USA.
Chiotti
Ruthanne
R
Goldstein
Richard E
RE
D000818:Animals; D001806:Blood Urea Nitrogen; D002371:Cat Diseases / Q000097:blood / Q000503:physiopathology; D002415:Cats / Q000097:blood / Q000502:physiology*; D002908:Chronic Disease; D003287:Contrast Media; D003404:Creatinine / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D019786:Gadolinium DTPA / Q000097:blood*; D005919:Glomerular Filtration Rate / Q000662:veterinary*; D007472:Iohexol / Q000032:analysis; D007674:Kidney Diseases / Q000097:blood / Q000503:physiopathology / Q000662:veterinary; D008297:Male; D015203:Reproducibility of Results
D003287:Contrast Media; D007472:Iohexol; D003404:Creatinine; D019786:Gadolinium DTPA