A facile scanometric strategy for ultrasensitive detection of protein using aptamer-initiated rolling circle amplification.
A pragmatic and simple strategy was developed for ultrasensitive detection of protein with good specificity and low matrix effect, which combined aptamer-initiated rolling circle amplification with a Au nanoparticle probe and convenient scanometric readout.
Cheng
Wei
W
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.
Ding
Lin
L
Chen
Yunlong
Y
Yan
Feng
F
Ju
Huangxian
H
Yin
Yibing
Y
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012834:Silver / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D052157:Aptamers, Nucleotide; D011506:Proteins; D042461:Vascular Endothelial Growth Factor A; D012834:Silver; D006046:Gold