[The comet assay application in radiobiological investigations].
The analysis of the literature data on application of the gel electrophoresis of individual cells ("comet assay") in radiobiological investigations was carried out. The descriptions of various variants of the method are presented; its alkaline version is in more detail considered. The works concerning to induction and DNA damage repair of single stranded and double stranded DNA breaks, DNA alkali labile sites, crosslinks, DNA bases damage, cellular radiosensitivity and revealing of apoptotic cells were analyzed. The application of the method at biomonitoring of DNA damage level in cells of the person and the animals exposed to genotoxic agents, including ionizing radiation is described. The analysis of the literary data testifies to perceptivity of development and further uses of this method in radiobiological researches.
Sirota
N P
NP
Kuznetsova
E A
EA
D000818:Animals; D020552:Comet Assay / Q000379:methods* / Q000706:statistics & numerical data*; D004249:DNA Damage; D004260:DNA Repair; D005069:Evaluation Studies as Topic; D006801:Humans; D011853:Radiobiology / Q000379:methods*