title: Inline ultrafiltration.
abstract: Inline ultrafiltration (UF) can significantly increase the recoverable mass of biopharmaceutical products when pool tank volumes are limiting. Using relatively small commercially available ultrafiltration cassettes, a proof-of-concept study demonstrates that inline UF can significantly increase recoverable mass in an antibody purification process. With ever-increasing cell culture titers pushing product masses to higher levels, inline UF offers a relatively easy-to-implement and less disruptive alternative to installing larger pool tanks and enables more cost-effective production utilizing facilities designed for smaller bulk sizes.
authors: Late Stage Purification Process R&D, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. cteske@gene.com
authors: Christopher A
authors: CA
authors: Teske
authors: Benedicte
authors: B
authors: Lebreton
authors: Robert
authors: R
authors: van Reis
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D014462:Ultrafiltration / Q000379:methods*