title: Structural basis for methylarginine-dependent recognition of Aubergine by Tudor.
abstract: Piwi proteins are modified by symmetric dimethylation of arginine (sDMA), and the methylarginine-dependent interaction with Tudor domain proteins is critical for their functions in germline development. Cocrystal structures of an extended Tudor domain (eTud) of Drosophila Tudor with methylated peptides of Aubergine, a Piwi family protein, reveal that sDMA is recognized by an asparagine-gated aromatic cage. Furthermore, the unexpected Tudor-SN/p100 fold of eTud is important for sensing the position of sDMA. The structural information provides mechanistic insights into sDMA-dependent Piwi-Tudor interaction, and the recognition of sDMA by Tudor domains in general.
authors: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors: Haiping
authors: H
authors: Liu
authors: Ju-Yu S
authors: JY
authors: Wang
authors: Ying
authors: Y
authors: Huang
authors: Zhizhong
authors: Z
authors: Li
authors: Weimin
authors: W
authors: Gong
authors: Ruth
authors: R
authors: Lehmann
authors: Rui-Ming
authors: RM
authors: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D017124:Conserved Sequence; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005854:Germ Cells / Q000254:growth & development / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D029721:Drosophila Proteins; D026901:Membrane Transport Proteins; D010448:Peptide Initiation Factors; C436124:aub protein, Drosophila; C071597:tud protein, Drosophila; C024917:symmetric dimethylarginine; D001120:Arginine