title: Effect of bone morphogenetic protein 4 in the human brain glioma cell line U251.
abstract: The role of bone morphogenetic protein 4 (BMP4) in gliomas is not clear. We hypothesized that BMP4 inhibits proliferation in the brain glioma cell line U251 through a signaling pathway involving BMP4 and the mothers against decapentaplegic homolog 4 (SMAD4) protein. We exposed U251 cells to Adriamycin (1 g) for 48 h; cell proliferation (MTT assay), expression of BMP4 and SMAD4 (mRNA: qPCR; protein: Western blot) were studied. We further altered expression of BMP4 by overexpression or siRNA silencing, and documented cell responses to Adriamycin. Proliferation of U251 cells was significantly inhibited upon exposure to Adriamycin. This inhibition was associated with increased expression of BMP4. Further, proliferation of U251 cells was inhibited when BMP4 was overexpressed. BMP4 expression negatively correlated with expression of SMAD4, such that elevated levels of BMP4 were associated with decreased expression of SMAD4 and vice versa. The Adriamycin-induced inhibition of proliferation of U251 cells was attenuated when BMP4 was knocked down by siRNA. To conclude, BMP4 is associated with inhibition of proliferation of U251 cells; the effects of BMP4 involve the BMP4-Smad signaling pathway. BMP4 has a potential as a target for glioma therapy.
authors: Renmin Hospital, Wuhan University, Wuhan 430060, Hubei, China.
authors: Baohui
authors: B
authors: Liu
authors: Daofeng
authors: D
authors: Tian
authors: Wei
authors: W
authors: Yi
authors: Liquan
authors: L
authors: Wu
authors: Qiang
authors: Q
authors: Cai
authors: Huimin
authors: H
authors: Dong
authors: Heng
authors: H
authors: Shen
authors: Baowei
authors: B
authors: Ji
authors: Long
authors: L
authors: Wang
authors: Shenqi
authors: S
authors: Zhang
authors: Dong
authors: D
authors: Ruan
authors: Qianxue
authors: Q
authors: Chen
mesh_terms: D055415:Bone Morphogenetic Protein 4 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020868:Gene Silencing; D005910:Glioma / Q000473:pathology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D051901:Smad4 Protein / Q000378:metabolism
chemical_list: C525809:BMP4 protein, human; D055415:Bone Morphogenetic Protein 4; D034741:RNA, Small Interfering; D051901:Smad4 Protein