title: Detection of airborne bacteria in a German turkey house by cultivation-based and molecular methods.
abstract: Today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. Depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. In this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the concentrations and the composition of bacterial community. Results obtained with the molecular approach showed clearly its applicability for qualitative exposure measurements. With both, cultivation-based and molecular methods species of microorganism with a potential health risk for employees (Acinetobacter johnsonii, Aerococcus viridans, Pantoea agglomerans, and Shigella flexneri) were identified. These results underline the necessity of adequate protection measures, including the recommendation to wear breathing masks during work in poultry houses.
authors: Institute for Applied Microbiology, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors: Kerstin
authors: K
authors: Fallschissel
authors: Kerstin
authors: K
authors: Klug
authors: Peter
authors: P
authors: Kämpfer
authors: Udo
authors: U
authors: Jäckel
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D056570:Aerococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000336:Aerosols / Q000032:analysis; D000391:Air Microbiology; D000395:Air Pollutants, Occupational / Q000032:analysis*; D016902:Air Pollution, Indoor / Q000032:analysis* / Q000706:statistics & numerical data; D000822:Animal Husbandry; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004391:Dust / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D005374:Filtration; D005858:Germany; D006799:Housing, Animal; D006801:Humans; D008297:Male; D008828:Microbiological Techniques / Q000379:methods; D008969:Molecular Sequence Data; D016273:Occupational Exposure / Q000032:analysis*; D020636:Pantoea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012763:Shigella flexneri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014422:Turkeys / Q000382:microbiology
chemical_list: D000336:Aerosols; D000395:Air Pollutants, Occupational; D004391:Dust; D012336:RNA, Ribosomal, 16S