Co-immunoprecipitation and protein blots.
Knowledge about the identity of the interacting partners is important for the understanding of the function and the cellular activity of a given protein. Here we describe co-immunoprecipitation and pull-down as methods that are widely used for the identification and characterization of protein-protein interactions. These methods are well suited to find or confirm the interaction among multiple proteins, given the availability of a specific antibody for or a tagged version of the protein of interest.
Isono
Erika
E
Department of Plant Systems Biology, Technical University Munich-Weihenstephan, Munich, Germany.
Schwechheimer
Claus
C
D015153:Blotting, Western / Q000379:methods; D015151:Immunoblotting / Q000379:methods; D047468:Immunoprecipitation / Q000379:methods*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*
D010940:Plant Proteins