Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas.
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to detect chromosomal imbalances by comparative genomic hybridization (CGH) in ectopic tissue samples from ovarian endometriomas and eutopic tissue from the same patients. We evaluated 10 ovarian endometriotic tissues and 10 eutopic endometrial tissues by metaphase CGH. CGH was prepared with normal and test DNA enzymatically digested, ligated to adaptors and amplified by PCR. A second PCR was performed for DNA labeling. Equal amounts of both normal and test-labeled DNA were hybridized in human normal metaphases. The Isis FISH Imaging System V 5.0 software was used for chromosome analysis. In both eutopic and ectopic groups, 4/10 samples presented chromosomal alterations, mainly chromosomal gains. CGH identified 11q12.3-q13.1, 17p11.1-p12, 17q25.3-qter, and 19p as critical regions. Genomic imbalances in 11q, 17p, 17q, and 19p were detected in normal eutopic and/or ectopic endometrium from women with ovarian endometriosis. These regions contain genes such as POLR2G, MXRA7 and UBA52 involved in biological processes that may lead to the establishment and maintenance of endometriotic implants. This genomic imbalance may affect genes in which dysregulation impacts both eutopic and ectopic endometrium.
Veiga-Castelli
L C
LC
Departamento de Genética, Universidade de São Paulo. luciana.veigacastelli@gmail.com
Silva
J C Rosa E
JC
Meola
J
J
Ferriani
R A
RA
Yoshimoto
M
M
Santos
S A
SA
Squire
J A
JA
Martelli
L
L
D000328:Adult; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010049:Ovarian Diseases / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction
D004247:DNA