title: Static magnetic fields enhanced the potency of cisplatin on k562 cells.
abstract: OBJECTIVE
  This study investigates whether 8.8 mT static magnetic fields (SMFs) can enhance the killing potency of cisplatin (DDP) on human leukemic cells (K562).
  
  
  METHODS
  The cell proliferation, cell cycle distribution, DNA damage, and the change in cell surface ultrastructure after K562 cells were exposed to 8.8 mT SMFs with or without DDP were analyzed.
  
  
  RESULTS
  The results show that SMFs enhanced the killing effect of DDP on K562 cells, reducing the efficient killing concentration of DDP on K562 cells from 20 to 10 microg/mL. Atomic force microscope observation showed that the cell surface ultrastructure was altered. The results of fluorescence-activated cell sorting analysis indicated that K562 cells treated with SMF plus DDP were arrested at the S phase. The SMF exposure induced DNA to become thicker than controls, and breakage of DNA occurred in the DDP group; however, DNA breakage was increased in the SMF + DDP group.
  
  
  CONCLUSIONS
  The results show that SMFs enhanced the anticancer effect of DDP on K562 cells. The mechanism correlated with the DNA damage model. This study also shows the potentiality of SMFs as an adjunctive treatment method for chemotherapy.
authors: Shaanxi Normal University, Xi'an, China.
authors: Wen-Fang
authors: WF
authors: Chen
authors: Hao
authors: H
authors: Qi
authors: Run-Guang
authors: RG
authors: Sun
authors: Ying
authors: Y
authors: Liu
authors: Kun
authors: K
authors: Zhang
authors: Jian-Qiang
authors: JQ
authors: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004357:Drug Synergism; D004361:Drug Tolerance / Q000528:radiation effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D055909:Magnetic Field Therapy; D018625:Microscopy, Atomic Force
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin