Bioactive molecules in Kalanchoe pinnata leaves: extraction, purification, and identification.
Kalanchoe pinnata (Lam.) Pers. (syn. Bryophyllum pinnatum; family Crassulaceae) is a popular plant used in traditional medicine in many temperate regions of the world and particularly in South America. In Guyana, the leaves are traditionally used as an anti-inflammatory and antiseptic to treat coughs, ulcers, and sores. The purpose of this study was to implement a method for targeting and identifying molecules with antimicrobial activity, which could replace chemical preservatives in cosmetic applications. The leaves were extracted by a method based on pressurized liquid extraction (PLE), using different solvents. A study of antimicrobial activity and cytotoxicity tests were performed to select the most interesting extract. To isolate one or more active molecules, the selected crude extract was fractionated by centrifugal partition chromatography (CPC) and then antimicrobial activity and cytotoxicity of each fraction were tested under the same procedure. The last step consisted of identifying the main compounds in the most active fraction by LC-MS/MS.
El Abdellaoui
Saïda
S
Institut de Chimie Organique et Analytique, Université d'Orléans-CNRS, UMR CNRS 6005, BP 67059, 45067, Orléans Cedex 2, France.
Destandau
Emilie
E
Toribio
Alix
A
Elfakir
Claire
C
Lafosse
Michel
M
Renimel
Isabelle
I
André
Patrice
P
Cancellieri
Perrine
P
Landemarre
Ludovic
L
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D029627:Crassulaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry
D000890:Anti-Infective Agents