[Sequence polymorphisms of mitochondrial DNA hypervariable 1 of peripheral blood cells from 47 HIV/AIDS patients of Han nationality].
OBJECTIVE
To investigate alterations of hyper variable region 1 (HR 1) of mitochondrial DNA Blood cells were (mtDNA) in white blood cells of Chinese Han nationality HIV/AIDS patients.


METHODS
obtained from 47 cases of therapy-naïve HIV/AIDS patients without opportunity infection and DNA were extracted using blood DNA extracted kit. About 600 bp fragments which contain HR 1 were amplified by PCR. Alterations were determined by directed DNA sequencing.


RESULTS
There were 124 polymorphism sites in mtDNA HR 1 (nb16024-16383) in 47 HIV/AIDS patients. The alteration rate was 0 to 20.47% (median 5.33%). 36 cases experienced C to T nucleotide change at nt 16 223, and the alteration rate was 70.97%. At nt 16 362, 26 individuals showed T to C nucleotide change and 3 individuals showed T to G alteration, alteration rate was 55.32% (26/47) and 6.38% (3/47) individually.


CONCLUSIONS
HIV infection may cause more alterations in HR 1 regions.
Wu
Ya-song
YS
Division of Treatment and Care, National Center for AIDS/STD Control and Prevention, China CDC, Beijing.
Chen
De-xi
DX
Chen
Xin-yue
XY
Shi
Ying
Y
Liu
Ya-li
YL
Zhang
Hong-hai
HH
Wu
Hao
H
D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D002681:China; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000208:ethnology / Q000235:genetics*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial