Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis.
BACKGROUND
The straight relationship between cirrhosis and impaired intestinal barrier has not been elucidated yet.


OBJECTIVE
To verify (51)Cr-EDTA-intestinal permeability in rats with CCl(4)-induced cirrhosis and controls.


METHODS
Fifty male Wistar rats weighing 150-180 g were separated in three groups: 25 animals received CCl(4) 0.25 mL/kg with olive oil by gavage with 12 g/rat/day food restriction for 10 weeks (CCl(4)-induced cirrhosis); 12 received the same food restriction for 10 weeks (CCl(4)-non exposed). Other 13 rats received indomethacin 15 mg/kg by gavage as positive control of intestinal inflammation.


RESULTS
The median (25-75 interquartile range) (51)Cr-EDTA-IP values of cirrhotic and CCl(4)-non exposed rats were 0.90% (0.63-1.79) and 0.90% (0.60-1.52) respectively, without significant difference (P = 0.65). Animals from indomethacin group showed (51)Cr-EDTA-IP, median 7.3% (5.1-14.7), significantly higher than cirrhotic and CCl(4)-non exposed rats (P<0.001).


CONCLUSIONS
This study showed the lack of difference between (51)Cr-EDTA-intestinal permeability in rats with and without cirrhosis. Further studies are necessary to better clarify the relationship between intestinal permeability and cirrhosis.
Ramos
Ana Regina L
AR
Experimental Laboratory of Gastroenterology and Hepatology, Hospital de Clínicas de Porto Alegre, Porto Alegre-RS, Brazil. anareginalimaramos@yahoo.com.br
Matte
Ursula
U
Goldani
Helena Ayako Sueno
HA
Oliveira
Osmar L M
OL
Vieira
Sandra Maria Gonçalves
SM
Silveira
Themis Reverbel da
TR
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002251:Carbon Tetrachloride; D004492:Edetic Acid / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004492:Edetic Acid; D002251:Carbon Tetrachloride; D007213:Indomethacin