Influence of solubilizing environments on membrane protein structures.
Membrane protein structures are stabilized by weak interactions and are influenced by additional interactions with the solubilizing environment. Structures of influenza virus A M2 protein, a proven drug target, have been determined in three different environments, thus providing a unique opportunity to assess environmental influences. Structures determined in detergents and detergent micelles can have notable differences from those determined in lipid bilayers. These differences make it imperative to validate membrane protein structures.
Cross
Timothy A
TA
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA. cross@magnet.fsu.edu
Sharma
Mukesh
M
Yi
Myunggi
M
Zhou
Huan-Xiang
HX
D000818:Animals; D003902:Detergents / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry; D007473:Ion Channels / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D000086082:Viroporin Proteins
D003902:Detergents; D007473:Ion Channels; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008823:Micelles; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins