title: Glucocorticoid receptor gene haplotype structure and steroid therapy outcome in IBD patients.
abstract: OBJECTIVE
  To study whether the glucocorticoid receptor (GR/NR3C1) gene haplotypes influence the steroid therapy outcome in inflammatory bowel disease (IBD).
  
  
  METHODS
  We sequenced all coding exons and flanking intronic sequences of the NR3C1 gene in 181 IBD patients, determined the single nucleotide polymorphisms, and predicted the NR3C1 haplotypes. Furthermore, we investigated whether certain NR3C1 haplotypes are significantly associated with steroid therapy outcomes.
  
  
  RESULTS
  We detected 13 NR3C1 variants, which led to the formation of 17 different haplotypes with a certainty of > 95% in 173 individuals. The three most commonly occurring haplotypes were included in the association analysis of the influence of haplotype on steroid therapy outcome or IBD activity. None of the NR3C1 haplotypes showed statistically significant association with glucocorticoid therapy success.
  
  
  CONCLUSIONS
  NR3C1 haplotypes are not related to steroid therapy outcome.
authors: Division of Clinical Pharmacology and Toxicology, University Hospital, Zurich, Switzerland.
authors: Jessica
authors: J
authors: Mwinyi
authors: Christa
authors: C
authors: Wenger
authors: Jyrki J
authors: JJ
authors: Eloranta
authors: Gerd A
authors: GA
authors: Kullak-Ublick
mesh_terms: D000328:Adult; D016009:Chi-Square Distribution; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000235:genetics*; D003424:Crohn Disease / Q000188:drug therapy* / Q000235:genetics*; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D007438:Introns; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D013256:Steroids / Q000627:therapeutic use*; D013557:Switzerland; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: C497713:NR3C1 protein, human; D011965:Receptors, Glucocorticoid; D013256:Steroids