Monoclonal antibodies against the PB1-F2 protein of H1N1 influenza A virus.
In this report we describe the generation of a mouse monoclonal antibody (MAb) against the influenza A virus PB1-F2 protein that is derived from a +1 reading frame of the polymerase basic protein (PB1) gene segment. We further present data that the hybridoma subclone F2-6G10 produces antibodies that specifically recognize the PB1-F2 protein of H1N1 influenza virus types only. The antibody can be used for immunodetection of the PB1-F2 protein in ELISA, Western blot, immunoprecipitation, and immunofluorescence assays.
Nordmann
Alexandra
A
Institute of Molecular Virology, Münster University Hospital Medical School, Münster, Germany.
Wixler
Ludmilla
L
Ludwig
Stephan
S
Wixler
Viktor
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002478:Cells, Cultured; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D047468:Immunoprecipitation; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; C442816:PB1-F2 protein, Influenza A virus; D011994:Recombinant Proteins; D014764:Viral Proteins