Comparative study of tumor hypoxia by diffuse optical spectroscopy and immunohistochemistry in two tumor models.
The capabilities of diffuse optical spectroscopy for noninvasive assessing of oxygen status in experimental tumors have been demonstrated. Specific features of the distribution of total hemoglobin, oxygenated hemoglobin, deoxygenated hemoglobin, and blood-oxygen saturation were shown on two tumor models having different histological structure and functional characteristics. The results obtained by the optical technique were verified by immunohistochemical study of tissue samples marked with exogenous marker of hypoxia--pimonidazole.
Maslennikova
Anna V
AV
Institute of Applied Physics RAS, 46 Ulyanov Str., Nizhny Novgorod, Russia; Nizhny Novgorod State Medical Academy, 10/1 Minin Sqr., Nizhny Novgorod, Russia. maslennikova.anna@gmail.com
Orlova
Anna G
AG
Golubiatnikov
German Yu
GY
Kamensky
Vladislav A
VA
Shakhova
Natalia M
NM
Babaev
Aleksey A
AA
Snopova
Ludmila B
LB
Ivanova
Irina P
IP
Plekhanov
Vladimir I
VI
Prianikova
Tatyana I
TI
Turchin
Ilya V
IV
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D004058:Diffusion; D004195:Disease Models, Animal; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods*; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D008495:Medical Oncology / Q000379:methods*; D009593:Nitroimidazoles; D055096:Optical Devices; D010100:Oxygen / Q000378:metabolism; D011838:Radiation-Sensitizing Agents; D051381:Rats; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
D014408:Biomarkers, Tumor; D006454:Hemoglobins; D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; C033815:pimonidazole; D010100:Oxygen