title: Mutations in the stalk region of the measles virus hemagglutinin inhibit syncytium formation but not virus entry.
abstract: Measles virus (MV) entry requires at least 2 viral proteins, the hemagglutinin (H) and fusion (F) proteins. We describe the rescue and characterization of a measles virus with a specific mutation in the stalk region of H (I98A) that is able to bind normally to cells but infects at a lower rate than the wild type due to a reduction in fusion triggering. The mutant H protein binds to F more avidly than the parent H protein does, and the corresponding virus is more sensitive to inhibition by fusion-inhibitory peptide. We show that after binding of MV to its receptor, H-F dissociation is required for productive infection.
authors: Department of Molecular Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
authors: Matthew K
authors: MK
authors: Ennis
authors: Chunling
authors: C
authors: Hu
authors: Shruthi K
authors: SK
authors: Naik
authors: Louay K
authors: LK
authors: Hallak
authors: Kah Whye
authors: KW
authors: Peng
authors: Stephen J
authors: SJ
authors: Russell
authors: David
authors: D
authors: Dingli
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015726:Giant Cells / Q000821:virology; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008459:Measles virus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020125:Mutation, Missense; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D053586:Virus Internalization
chemical_list: D006389:Hemagglutinins, Viral; D050505:Mutant Proteins; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus