title: Zhihengliuella aestuarii sp. nov., isolated from tidal flat sediment.
abstract: A Gram-stain-positive, ovoid to short rod-shaped, yellow-pigmented bacterium, designated strain DY66(T), was isolated from tidal-flat sediment collected from Deukryang Bay (Republic of Korea), and its taxonomic position was investigated by using a polyphasic approach. Strain DY66(T) grew optimally at 30 °C and pH 8-9 and in 2 % (w/v) NaCl. The peptidoglycan type was A4α, L-Lys-L-Ala-D-Glu, and tyvelose and glucose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10 % of total) were anteiso-C(15 : 0) and iso-C(15 : 0). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown aminophospholipid and three unknown aminolipids. The DNA G+C content was 59.1 mol%. This chemotaxonomic profile supported the assignment of strain DY66(T) to the genus Zhihengliuella. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain DY66(T) belonged to the family Micrococcaceae and was related to the genus Zhihengliuella. On the basis of the evidence presented in this study, strain DY66(T) represents a novel species of the genus Zhihengliuella, for which the name Zhihengliuella aestuarii sp. nov. is proposed. The type strain is strain DY66(T) ( = KCTC 19557(T)  = JCM 16364(T)).
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Keun Sik
authors: KS
authors: Baik
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Chae Hong
authors: CH
authors: Lim
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Seong Chan
authors: SC
authors: Park
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Han Na
authors: HN
authors: Choe
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Ho Jun
authors: HJ
authors: Kim
authors: Department of Food Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: DuWoon
authors: D
authors: Kim
authors: Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong, Daejeon 305-333, Republic of Korea.
authors: Kang Hyun
authors: KH
authors: Lee
authors: Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea.
authors: Chi Nam
authors: CN
authors: Seong
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2