Genotyping of Aeromonas hydrophila by box elements.
PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500-3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers.
Singh
Vijai
V
National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226002, India. vijaisingh15@gmail.com
Chaudhary
Dharmendra Kumar
DK
Mani
Indra
I
Somvanshi
Pallavi
P
Rathore
Gaurav
G
Sood
Neeraj
N
D016980:Aeromonas hydrophila / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial