Phylogenetic substitution models for detecting heterotachy during plastid evolution.
There is widespread evidence of lineage-specific rate variation, known as heterotachy, during protein evolution. Changes in the structural and functional constraints acting on a protein can lead to heterotachy, and it is plausible that such changes, known as covarion shifts, may affect many amino acids at once. Several previous attempts to model heterotachy have used covarion models, where the sequence undergoes covarion drift, whereby each site may switch independently among a set of discrete classes having different substitution rates. However, such independent switching may not capture biologically important events where the selective forces acting on a protein affect many sites at once. We describe a new class of models that allow the rates of substitution and switching to vary among branches of a phylogenetic tree. Such models are better able to handle covarion shifts. We apply these models to a set of genes occurring in nonphotosynthetic bacteria, cyanobacteria, and the plastids of green and red algae. We find that 4/5 genes show evidence of some form of rate switching and that 3/5 genes show evidence that the relative switching rate differs among taxonomic groups. We conclude that covarion shifts may be frequent during the deep evolution of plastid genes and that our methodology may provide a powerful new tool for investigating such shifts in other systems.
Whelan
Simon
S
Computational and Evolutionary Biology, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom. simon.whelan@manchester.ac.uk
Blackburne
Benjamin P
BP
Spencer
Matthew
M
D000465:Algorithms; D001483:Base Sequence; D005075:Biological Evolution; D000460:Chlorophyta / Q000166:cytology / Q000235:genetics; D003198:Computer Simulation; D014644:Genetic Variation; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D018087:Plastids / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D000461:Rhodophyta / Q000166:cytology / Q000235:genetics; D016415:Sequence Alignment
D011506:Proteins