title: Platelets prevent acute liver damage after extended hepatectomy in pigs.
abstract: OBJECTIVE
  Platelets develop tissue repair and promote liver regeneration. We investigated whether platelets prevented acute liver damage after extended hepatectomy in pigs.
  
  
  METHODS
  Thrombocytosis was induced by the following two methods; afterwards 80% hepatectomy was performed in pigs. In the first method, the pigs received administration of thrombopoietin [TPO (+) group], and they were compared with a control group [TPO (-) group]. In the second method, the pigs received a splenectomy [Sp (+) group], and theywere compared with another control group [Sp (-) group]. Platelet counts, biochemical examination of blood, and histopathological findings of the residual liver were examined.
  
  
  RESULTS
  Serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin (T-Bil) levels were significantly decreased in the thrombocytotic groups compared with the control groups in the early period after hepatectomy. In the histopathological findings, hemorrhagic necrosis with a bile plug was observed in the control groups, but this phenomenon was not observed in the thrombocytotic groups. On transmission electron microscopy, the sinusoidal endothelial lining was destroyed and detached into the sinusoidal space with enlargement of Disse's spaces in the thrombocytotic groups, but these findings were not observed in the control groups.
  
  
  CONCLUSIONS
  An increased number of platelets prevents acute liver damage after extended hepatectomy.
authors: Department of Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8575, Japan.
authors: Katsuji
authors: K
authors: Hisakura
authors: Soichiro
authors: S
authors: Murata
authors: Kiyoshi
authors: K
authors: Fukunaga
authors: Andriy
authors: A
authors: Myronovych
authors: Sosuke
authors: S
authors: Tadano
authors: Takuya
authors: T
authors: Kawasaki
authors: Keisuke
authors: K
authors: Kohno
authors: Osamu
authors: O
authors: Ikeda
authors: Sugiru
authors: S
authors: Pak
authors: Naoya
authors: N
authors: Ikeda
authors: Yoritaka
authors: Y
authors: Nakano
authors: Ryota
authors: R
authors: Matsuo
authors: Kenjiro
authors: K
authors: Konno
authors: Eiji
authors: E
authors: Kobayashi
authors: Toshiyuki
authors: T
authors: Saito
authors: Hiroshi
authors: H
authors: Yasue
authors: Nobuhiro
authors: N
authors: Ohkohchi
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001792:Blood Platelets / Q000502:physiology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006498:Hepatectomy / Q000009:adverse effects*; D010976:Platelet Count; D013156:Splenectomy / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D013922:Thrombocytosis / Q000097:blood*; D013926:Thrombopoietin / Q000008:administration & dosage / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D013926:Thrombopoietin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin