Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with hydrosheets composed of chitin/chitosan, fucoidan, and alginate as wound dressings.
To create a moist environment for rapid wound healing, a hydrosheet composed of alginate, chitin/chitosan, and fucoidan (ACF-HS) has been developed as a functional wound dressing. The aim of this study was to evaluate the accelerating effect of ACF-HS on wound healing for rat mitomycin C-treated healing-impaired wounds. Full-thickness skin defects were made on the back of rats and mitomycin C was applied onto the wound for 10 minutes to prepare a healing-impaired wound. After thoroughly washing out the mitomycin C, ACF-HS was applied to the healing-impaired wounds. The rats were later euthanized and histological sections of the wounds were prepared. The histological examinations showed significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ACF-HS on days 7 and 14, in comparison with that in alginate fiber (Kaltostat), hydrogel wound dressing (DuoACTIVE), and nontreatment (negative control). Furthermore, in cell culture studies, ACF-HS-absorbed serum and fibroblast growth factor-2 was found to be proliferative for fibroblasts and endothelial cells, respectively, and ACF-HS-absorbed serum was found to be chemoattractive for fibroblasts. However, our results may not be strictly comparable with general healing-impaired wound models in humans because of the cell damage by mitomycin C. In addition, more biocompatibility studies of fucoidan are essential due to the possibility of renal toxicity.
Murakami
Kaoru
K
Department of Oral and Maxillofacial Surgery Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan. dr22056@ndmc.ac.jp
Ishihara
Masayuki
M
Aoki
Hiroshi
H
Nakamura
Shingo
S
Nakamura
Shin-Ichiro
S
Yanagibayashi
Satoshi
S
Takikawa
Megumi
M
Kishimoto
Satoko
S
Yokoe
Hidetaka
H
Kiyosawa
Tomoharu
T
Sato
Yasunori
Y
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology; D002478:Cells, Cultured; D002686:Chitin / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D020405:Dermis / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology; D013463:Sulfuric Acid Esters; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000473:pathology / Q000517:prevention & control; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
D000464:Alginates; D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D019384:Nucleic Acid Synthesis Inhibitors; D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D002686:Chitin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016685:Mitomycin; D020723:Glucuronic Acid; D048271:Chitosan; C007789:fucoidan