Simultaneous determination of albendazole and praziquantel by second derivative spectrophotometry and multivariated calibration methods in veterinary pharmaceutical formulation.
The simultaneous determination of albendazole (ABZ) and praziquantel (PZQ) was performed by different mathematical approaches: second derivative spectrophotometry (SDS), classical least squares, regression of partial least squares and principal components regression based on spectral data of drugs dissolved in methanol-hydrochloric acid solution. The detection limits for multivariate calibrations were determined by creating a surrogate variable signal. SDS presented the best analytical features. The recoveries of ABZ and PZQ from the synthetic samples were near to 100 +/- 5%. The methods were applied in veterinary pharmaceutical formulation whose mass ratio ABZ:PZQ is 10:1; the results obtained were according to nominal content.
Soto
César
C
Department of Analytical and Inorganic Chemistry, Faculty of Chemical Sciences, University of Concepción, Chile. cesarsoto@udec.cl
Contreras
David
D
Orellana
Sandra
S
Yañez
Jorge
J
Toral
M Inés
MI
D015766:Albendazole / Q000032:analysis*; D000818:Animals; D006851:Hydrochloric Acid; D016018:Least-Squares Analysis; D057230:Limit of Detection; D000432:Methanol; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011223:Praziquantel / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
D004364:Pharmaceutical Preparations; D019155:Veterinary Drugs; D011223:Praziquantel; D015766:Albendazole; D006851:Hydrochloric Acid; D000432:Methanol