title: Heterogeneous thermal-lens immunoassay for small organic compounds: determination of 4-aminophenol.
abstract: 4-Aminophenol was selected as a model hapten for thermal-lens detection in a heterogeneous indirect immunoassay for small organic compounds. The assay is based on the competition of the free hapten and 4-aminophenol labeled with tetramethyl rhodamine isothiocyanate (TRITC) for rabbit anti-4-aminophenol antibodies (4-AP-GA-BSA) immobilized on a transparent poly(ethylene terephthalate) plate. The amount of the colored analyte was directly measured by thermal lensing at the plate surface. The developed method is specific for 4-aminophenol. The limit of detection is 2 x 10(-7) M, which corresponds to the absolute amount of 3 x 10(-11) mol of 4-aminophenol. The sensitivities of the developed thermal-lens and fluorescence-based measurements of the immunoassay under the same conditions are in good agreement with the expected and discussed theoretical sensitivities of photothermal and fluorescence measurements.
authors: Chemistry Department, M.V. Lomonosov Moscow State University, Leninskie Gory, 119991, GSP-1 Moscow, Russia.
authors: Dmitry A
authors: DA
authors: Nedosekin
authors: Anton V
authors: AV
authors: Brusnichkin
authors: Andrei Y
authors: AY
authors: Luk'yanov
authors: Sergei A
authors: SA
authors: Eremin
authors: Mikhail A
authors: MA
authors: Proskurnin