[Multilayer analysis of signal transduction and cell cycle control in GIST. Identifying new interaction partners with differential regulation].
To identify new interactions as well as diagnostically, prognostically and therapeutically relevant differences in the regulation of gene expression in gastrointestinal stromal tumors (GISTs), we analyzed the methylation status, mRNA expression, microRNA expression, protein expression and protein phosphorylation in parallel in identical tumor tissue samples. The data were analyzed in a multilayer approach and were correlated to each other and to clinico-pathological parameters. Differentially regulated genes were mapped to signal transduction pathways which are already known to play a major role in GISTs. A functionally orientated overview of the different data layers was constructed, which enabled new insights into gene regulation in GISTs.
Haller
F
F
Institut für allgemeine Pathologie und pathologische Anatomie, Universitätsklinikum Freiburg, Breisacher Str. 115a, 79106 Freiburg. florian.haller@uniklinik-freiburg.de
Zhang
D J
DJ
Löbke
C
C
von Heydebreck
A
A
Korf
U
U
Füzesi
L
L
Sahin
O
O
D002453:Cell Cycle / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D055361:Multilevel Analysis; D009363:Neoplasm Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D013223:Statistics as Topic
D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger