title: Chronic resveratrol intake reverses pro-inflammatory cytokine profile and oxidative DNA damage in ageing hybrid mice.
abstract: Thymic involution and shrinkage of secondary lymphoid organs are leading causes of the deterioration of the T-cell compartment with age. Inflamm-aging, a sustained inflammatory status, has been associated with chronic diseases and shortened longevity. This is the first study to investigate the effect of treating aging hybrid mice with long-term, low-dose resveratrol (RSV) in drinking water by assessing multiple immunological markers and profiles in the immune system. We found that hybrid mice exhibited marked age-related changes in the CD3+CD4+, C3+CD8+, CD4+CD25+, CD4M and CD8M surface markers. RSV reversed surface phenotypes of old mice to that of young mice by maintaining the CD4+ and CD8+ population in splenocytes as well as reducing CD8+CD44+ (CD8M) cells in the aged. RSV also enhanced the CD4+CD25+ population in old mice. Interestingly, pro-inflammatory status in young mice was transiently elevated by RSV but it consequently mitigated the age-dependent increased pro-inflammatory cytokine profile while preserving the anti-inflammatory cytokine condition in the old mice. Age-dependent increase in 8OHdG, an oxidative DNA damage marker was ameliorated by RSV. Immunological-focused microarray gene expression analysis showed that only the CD72 gene was significantly downregulated in the 12-month RSV-treated mice compared to age-matched controls. Our study indicates that RSV even at low physiological relevant levels is able to affect the immune system without causing marked gene expression changes.
authors: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore. ytwong@alumni.nus.edu.sg
authors: Yee Ting
authors: YT
authors: Wong
authors: Jan
authors: J
authors: Gruber
authors: Andrew M
authors: AM
authors: Jenner
authors: Francis Eng Hock
authors: FE
authors: Tay
authors: Runsheng
authors: R
authors: Ruan
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D000975:Antioxidants / Q000494:pharmacology*; D002452:Cell Count; D016207:Cytokines / Q000276:immunology*; D004249:DNA Damage / Q000187:drug effects*; D007249:Inflammation / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D000077185:Resveratrol; D013154:Spleen / Q000276:immunology; D013267:Stilbenes / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D000975:Antioxidants; D016207:Cytokines; D013267:Stilbenes; D000077185:Resveratrol