Proliferative and protective effects of SurR9-C84A on differentiated neural cells.
Targeting survivin has the ability to inhibit apoptosis and regulate mitosis for the protection of neuronal cells, and it offers several advantages for neuronal repair and protection. We found that the BIR motif mutant of survivin (SurR9-C84A) can bind to microtubules and regulate their stability, induce cell division, increase proliferation and activate the expression of cell cycle and neuronal markers in differentiated SK-N-SH and HCN-2 neurons. We further showed the protective effects of SurR9-C84A against post differentiation retinoic acid induced neurotoxicity. These abilities of SurR9-C84A offer a great potential for future neuronal repair therapy.
Baratchi
Sara
S
Institute of Biotechnology (BioDeakin), Institute for Technology Research and Innovation (ITRI), Deakin University, Australia.
Kanwar
Rupinder K
RK
Cheung
Chun Hei Antonio
CH
Kanwar
Jagat R
JR
D000409:Alanine / Q000235:genetics; D020816:Amino Acid Motifs / Q000235:genetics / Q000276:immunology; D019943:Amino Acid Substitution / Q000235:genetics / Q000276:immunology; D000818:Animals; D001120:Arginine / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003545:Cysteine / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008870:Microtubules / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D012097:Repressor Proteins / Q000235:genetics* / Q000502:physiology; D000077022:Survivin; D014212:Tretinoin / Q000037:antagonists & inhibitors / Q000633:toxicity
C117749:Birc5 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D012097:Repressor Proteins; D000077022:Survivin; D014212:Tretinoin; D001120:Arginine; D003545:Cysteine; D000409:Alanine