[Norovrius genotype I.8 being detected from sporadic cases of acute gastroenteritis in Nanning municipal].
OBJECTIVE
To study the genic characteristic of Norovrius genotype I.8 from sporadic gastroenteritis of a hospital in Nanning Municipal.


METHODS
Specimens of diarrhea patients from a hospital in Nanning Municipal between January 2007 and December 2008 were collected and detected for Noroviral genogroup Iribonucleic acid (RNA) by real-time reverse transcription-polymerase chain reaction(real-time RT-PCR). All positive specimens were subjected for sequencing analysis and constructed phylogenetic tree.


RESULTS
Norovirus genogroup I RNA were detected in 3 of 696 stool specimens. The homology between one of the four strains (NN07230) and reference strain of G I.8 (WUG I/00/JP) was 94.5%; and the homology between the NN07230 strain and strains circulating during 2006-2007 in Japan and Korea ranged from 96.7% to 98.5%. And they formed one cluster in the phylogenetic tree.


CONCLUSIONS
The research showed that genotype I.8 infection exists in sporadic gastroenteritis in Nanning City.
Deng
Li-li
LL
Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, Guangxi Zhuang Autonomous Region, China.
Liu
Wei
W
Tan
Dong-mei
DM
D000208:Acute Disease / Q000453:epidemiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D055815:Young Adult