Bacteriophage lysin mediates the binding of streptococcus mitis to human platelets through interaction with fibrinogen.
The binding of bacteria to human platelets is a likely central mechanism in the pathogenesis of infective endocarditis. We have previously found that platelet binding by Streptococcus mitis SF100 is mediated by surface components encoded by a lysogenic bacteriophage, SM1. We now demonstrate that SM1-encoded lysin contributes to platelet binding via its direct interaction with fibrinogen. Far Western blotting of platelets revealed that fibrinogen was the major membrane-associated protein bound by lysin. Analysis of lysin binding with purified fibrinogen in vitro confirmed that these proteins could bind directly, and that this interaction was both saturable and inhibitable. Lysin bound both the Aalpha and Bbeta chains of fibrinogen, but not the gamma subunit. Binding of lysin to the Bbeta chain was further localized to a region within the fibrinogen D fragment. Disruption of the SF100 lysin gene resulted in an 83+/-3.1% reduction (mean +/- SD) in binding to immobilized fibrinogen by this mutant strain (PS1006). Preincubation of this isogenic mutant with purified lysin restored fibrinogen binding to wild type levels. When tested in a co-infection model of endocarditis, loss of lysin expression resulted in a significant reduction in virulence, as measured by achievable bacterial densities (CFU/g) within vegetations, kidneys, and spleens. These results indicate that bacteriophage-encoded lysin is a multifunctional protein, representing a new class of fibrinogen-binding proteins. Lysin appears to be cell wall-associated through its interaction with choline. Once on the bacterial surface, lysin can bind fibrinogen directly, which appears to be an important interaction for the pathogenesis of endocarditis.
Seo
Ho Seong
HS
Division of Infectious Diseases, Veterans Affairs Medical Center and the University of California, San Francisco, California, United States of America.
Xiong
Yan Q
YQ
Mitchell
Jennifer
J
Seepersaud
Ravin
R
Bayer
Arnold S
AS
Sullam
Paul M
PM
D000595:Amino Acid Sequence; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000382:microbiology*; D015153:Blotting, Western; D004697:Endocarditis, Bacterial / Q000378:metabolism / Q000821:virology; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013290:Streptococcal Infections / Q000378:metabolism* / Q000821:virology; D017108:Streptococcus Phages / Q000235:genetics / Q000378:metabolism; D034361:Streptococcus mitis / Q000472:pathogenicity* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
D009088:Mucoproteins; D014764:Viral Proteins; C010393:lysin, gastropoda; D005340:Fibrinogen