title: The AP-3 β adaptin mediates the biogenesis and function of lytic vacuoles in Arabidopsis.
abstract: Plant vacuoles are essential multifunctional organelles largely distinct from similar organelles in other eukaryotes. Embryo protein storage vacuoles and the lytic vacuoles that perform a general degradation function are the best characterized, but little is known about the biogenesis and transition between these vacuolar types. Here, we designed a fluorescent marker-based forward genetic screen in Arabidopsis thaliana and identified a protein affected trafficking2 (pat2) mutant, whose lytic vacuoles display altered morphology and accumulation of proteins. Unlike other mutants affecting the vacuole, pat2 is specifically defective in the biogenesis, identity, and function of lytic vacuoles but shows normal sorting of proteins to storage vacuoles. PAT2 encodes a putative β-subunit of adaptor protein complex 3 (AP-3) that can partially complement the corresponding yeast mutant. Manipulations of the putative AP-3 β adaptin functions suggest a plant-specific role for the evolutionarily conserved AP-3 β in mediating lytic vacuole performance and transition of storage into the lytic vacuoles independently of the main prevacuolar compartment-based trafficking route.
authors: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
authors: Elena
authors: E
authors: Feraru
authors: Tomasz
authors: T
authors: Paciorek
authors: Mugurel I
authors: MI
authors: Feraru
authors: Marta
authors: M
authors: Zwiewka
authors: Ruth
authors: R
authors: De Groodt
authors: Riet
authors: R
authors: De Rycke
authors: Jürgen
authors: J
authors: Kleine-Vehn
authors: Jirí
authors: J
authors: Friml
mesh_terms: D033963:Adaptor Protein Complex 3 / Q000235:genetics / Q000378:metabolism*; D033966:Adaptor Protein Complex beta Subunits / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D009154:Mutation; D021381:Protein Transport; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D033963:Adaptor Protein Complex 3; D033966:Adaptor Protein Complex beta Subunits; D029681:Arabidopsis Proteins