title: Guanosine quadruplexes in solution: a small-angle x-ray scattering analysis of temperature effects on self-assembling of deoxyguanosine monophosphate.
abstract: We investigated quadruplex formation in aqueous solutions of 2'-deoxyriboguanosine 5'-monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics.
authors: Physical Science Section, Research Unit of Ancona, SAIFET Department, CNISM, Marche Polytechnic University, Via Brecce Bianche, 60131 Ancona, Italy.
authors: P
authors: P
authors: Mariani
authors: F
authors: F
authors: Spinozzi
authors: F
authors: F
authors: Federiconi
authors: M G
authors: MG
authors: Ortore
authors: H
authors: H
authors: Amenitsch
authors: L
authors: L
authors: Spindler
authors: I
authors: I
authors: Drevensek-Olenik