title: [Comparison of the immnunogenicity of rAAV2/1 and rAd5 rad5 expressing HIV-1 gag].
abstract: OBJECTIVE
  To compare the immunogenicity of rAAV2/1 and rAd5 expressing HIV-1 gag in BALB/c mice.
  
  
  METHODS
  BALB/c mice were immunized with rAAV2/1-gag or rAd5-gag once or twice. HIV-1 specific cellular immune responses were analyzed by in vivo CTL and intracellular cytokine staining assays. HIV-1 Gag specific antibodies were tested by ELISA.
  
  
  RESULTS
  Mice immunized with rAd5-gag once induced stronger Gag specific cellular immune responses and similar level of Gag specific antibody compared with rAAV2/1-gag. Mice immunized with rAd5-gag reached the peak immune responses more rapidly than rAAV2/1-gag. However, mice immunized with rAAV2/1-gag twice elicited better Gag specific IgG.
  
  
  CONCLUSIONS
  rAd5-gag induced strong HIV-1 specific cellular and antibody responses, and rAAV2/1-gag induced high level of HIV-1 specific IgG and moderate cellular immune responses.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors: Shuang-qing
authors: SQ
authors: Yu
authors: Xia
authors: X
authors: Feng
authors: Hong-mei
authors: HM
authors: Liu
authors: Hai-ru
authors: HR
authors: Yang
authors: Hong-xia
authors: HX
authors: Li
authors: Yi
authors: Y
authors: Zeng
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D054301:gag Gene Products, Human Immunodeficiency Virus