Stabilization of a nutraceutical omega-3 fatty acid by encapsulation in ultrathin electrosprayed zein prolamine.
Docosahexaenoic acid (DHA) is a long chain polyunsaturated fatty acid of the omega-3 series (omega-3), which exerts strong positive influences on human health. The target of this study was the stabilization by encapsulation of this bioactive ingredient in zein ultrathin capsules produced by electrospraying. The zein ultrathin DHA encapsulation was observed by ATR-FTIR spectroscopy to be more efficient against degradation under both ambient conditions and in a confined space (so-called headspace experiment). In the latter case, that more closely simulates a sealed food packaging situation, the bioactive DHA was considerably more stable. By fitting the degradation data to a specific auto-decomposition food lipids kinetic model, it was seen that the encapsulated omega-3 fatty acid showed a 2.5-fold reduction in the degradation rate constant and also had much higher degradation induction time. Moreover, the ultrathin zein-DHA capsules resulted to be more stable across relative humidity and temperature. Finally, headspace analysis by gas chromatography coupled with mass spectrometry showed that the presence of 3 main flavor-influencing aldehydes in the headspace was much lower in the zein encapsulated DHA, suggesting that the encapsulated bioactive also releases much less off-flavors. Electrosprayed ultrathin capsules of zein are shown to exhibit potential in the design of novel functional foods or bioactive packaging strategies to enhance the stability of functional ingredients. Practical Application: This article presents a novel methodology for the stabilization by encapsulation of omega 3 nutraceuticals via electrospraying and has potential application in the development of functional foods.
Torres-Giner
Sergio
S
Novel Materials and Nanotechnology Group, IATA, CSIC, Burjassot, Spain.
Martinez-Abad
Antonio
A
Ocio
Maria J
MJ
Lagaron
Jose M
JM
D000447:Aldehydes / Q000032:analysis / Q000737:chemistry; D019587:Dietary Supplements / Q000032:analysis*; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000737:chemistry; D055664:Electrochemical Techniques; D015525:Fatty Acids, Omega-3 / Q000032:analysis / Q000737:chemistry*; D005524:Food Technology / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006358:Hot Temperature / Q000009:adverse effects; D006813:Humidity / Q000009:adverse effects; D007700:Kinetics; D008956:Models, Chemical; D053769:Nanocapsules / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010665:Phenylpropanolamine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013649:Taste; D055549:Volatile Organic Compounds / Q000032:analysis / Q000737:chemistry; D015028:Zein / Q000737:chemistry*
D000447:Aldehydes; D015525:Fatty Acids, Omega-3; D053769:Nanocapsules; D055549:Volatile Organic Compounds; D004281:Docosahexaenoic Acids; D010665:Phenylpropanolamine; D015028:Zein