Inhibition of glucose intestinal absorption by kaempferol 3-O-α-rhamnoside purified from Bauhinia megalandra leaves.
Glucose intestinal absorption (GIA) is one of the factors that increase glycemia. Its reduction could be an important factor in decreasing hyperglycemia in diabetic patients. It has been shown that the aqueous extract of Bauhinia megalandra leaves inhibits GIA. In the present study we identified a compound present in the extract of B. megalandra responsible for the biological effect. The methanol extract of B. megalandra leaves was fractionated using different solvents, and high-speed counter-current chromatography yielding two pure compounds identified by (1)H NMR and (13)C NMR as kaempferol 3-O-α-rhamnoside and quercetin 3-O-α-rhamnoside. The first one increased the K(M) without changes in the V(MAX) of GIA. In addition it exerted an additive inhibitory effect, on GIA, when combined with phlorizin. We suggest that kaempferol 3-O-α-rhamnoside is a competitive inhibitor of intestinal SGLT1 cotransporter.
Rodríguez
Patricia
P
Sección de Bioquímica Médica, Instituto de Medicina Experimental, Facultad de Medicina, Universidad Central de Venezuela Apartado Postal 5058, Caracas, Venezuela.
González-Mujica
Freddy
F
Bermúdez
Jairo
J
Hasegawa
Masahisa
M
D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism* / Q000493:pharmacokinetics; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D044949:Kaempferols / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D006027:Glycosides; D044949:Kaempferols; C470066:kaempferol 3-O-rhamnoside; D005947:Glucose