[Human papillomavirus type 16 E5 sequence evolution analysis].
OBJECTIVE
To investigate the distribution of HPV 16 variants in Han women patients without Cervical intraepithelial neoplasia in the diagnosis and treatment center for cervical disease, department of Obstetrics and Gynecology in China-Japan friendship hospital with HPV 16 E5 sequence phylogenetic analysis.


METHODS
PCR amplification of HPV 16 E5 sequences and sequenced. The association between variations types and different cervical lesions was analyzed.


RESULTS
In this research, We first found that variant classification based on HPV 16 E5 DNA sequence (236 bp) alone had high rate of accuracy. In addition, for the first time, our research revealed that single-4075T can distinguished the As variant from all other variants.


CONCLUSIONS
If E5 sequence was used for phylogenetic analysis, it will greatly reduce the experimental costs and improve efficiency and cost-effectiveness. For the first time, our research revealed that single 4075T can distinguished the As variant from all other variants.
Hao
Min
M
Graduate School of Peking Union Medical College, Beijing 100730, China.
Wu
Xiao-zhou
XZ
Wang
Xiao-li
XL
Bian
Mei-lu
ML
Liu
Hong-tu
HT
D000328:Adult; D002584:Cervix Uteri / Q000821:virology; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D052162:Human papillomavirus 16 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D010802:Phylogeny; D055815:Young Adult; D000086082:Viroporin Proteins
D009856:Oncogene Proteins, Viral; C054859:oncogene protein E5, Human papillomavirus type 16; D000086082:Viroporin Proteins