title: Oral bacterial genome sequencing using the high-throughput Roche Genome Sequencer FLX System.
abstract: For over 30 years, the chain termination method of DNA sequencing (commonly known as Sanger sequencing) has been the mainstay of any DNA sequencing project. In the past, whole-genome sequencing employing exclusively Sanger chemistry has been a labor-intensive and costly exercise and an option unfeasible for the average research group. However, within the last 4 years, the introduction of three high-throughput sequencing technologies (454, SOLiD, and Illumina) has revolutionized genomics by facilitating unprecedented levels (up to gigabasepairs) of reliable DNA sequence output in a relatively short time frame and at a much lower cost per sequenced basepair. Here, we provide laboratory and bioinformatic protocols that will allow the average research group to undertake high-throughput sequencing of oral bacterial genomes using the Roche Genome Sequencer FLX System which employs 454 pyrosequencing technology.
authors: Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, Dunedin, New Zealand.
authors: Nicholas C K
authors: NC
authors: Heng
authors: Jo-Ann L
authors: JA
authors: Stanton
mesh_terms: D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics; D017422:Sequence Analysis, DNA; D012984:Software; D013291:Streptococcus / Q000235:genetics