Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties.
The present study was designed to identify phenotypically and genotypically two Arcanobacterium (A.) pyogenes strains isolated by post mortem examinations of a bearded dragon and a gecko. The A. pyogenes strains showed the typical biochemical properties and displayed CAMP-like synergistic hemolytic activities with various indicator strains. The species identity could be confirmed genotypically by amplification and sequencing of the 16S rDNA gene and, as novel target gene, by sequencing of the beta subunit of RNA polymerase encoding gene rpoB, of both strains and of reference strains representing nine species of the genus Arcanobacterium. The species identity of the two A. pyogenes strains could additionally be confirmed by PCR mediated amplification of species specific parts of the 16S-23S rDNA intergenic spacer region, the pyolysin encoding gene plo and by amplification of the collagen-binding protein encoding gene cbpA. All these molecular targets might help to improve the future identification and further characterization of A. pyogenes which, as demonstrated in the present study, could also be isolated from reptile specimens.
Ulbegi-Mohyla
H
H
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giebetaen, Frankfurter Str. 107, 35392 Giebetaen, Germany.
Hijazin
M
M
Alber
J
J
Lämmler
C
C
Hassan
A A
AA
Abdulmawjood
A
A
Prenger-Berninghoff
E
E
Weiss
R
R
Zschöck
M
M
D000818:Animals; D055667:Arcanobacterium / Q000166:cytology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003933:Diagnosis; D005838:Genotype; D006460:Hemolysin Proteins / Q000235:genetics; D008116:Lizards / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C490083:CbpA protein, bacteria; D017931:DNA Primers; D004275:DNA, Ribosomal; D006460:Hemolysin Proteins; C108448:Pyolysin, Arcanobacterium pyogenes