miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 during post-natal epididymal development in juvenile rats.
The unique temporal expression pattern of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper. It was found that miR-200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease. The pattern was exactly inverse to that of mRNA expression of transcription factor 8 (TCF8) revealed by quantitative real-time polymerase chain reaction (qRT-PCR), providing an extra evidence that TCF8 is one degradation target of miR-200c even in epididymis. Moreover, the qRT-PCR study on expression of E-cadherin and interleukin-2 indicated that miR-200c does exert an obvious effect on the mRNA expression of E-cadherin by directly regulating the mRNA level of TCF8, although the effect on interleukin-2 is not obvious as on E-cadherin, which implicates that interleukin-2 may be also regulated by other factors besides TCF8 in rat epididymis.
Wang
Junfeng
J
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
Ruan
Kangcheng
K
D000818:Animals; D015820:Cadherins / Q000235:genetics*; D016000:Cluster Analysis; D004822:Epididymis / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D000071799:Zinc Finger E-box-Binding Homeobox 1
D015820:Cadherins; D018398:Homeodomain Proteins; D007376:Interleukin-2; C555600:MIRN200 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger; D014157:Transcription Factors; C000630720:ZEB1 protein, rat; D000071799:Zinc Finger E-box-Binding Homeobox 1