title: Huperzine alkaloids from Australasian and southeast Asian Huperzia.
abstract: BACKGROUND
  The pharmaceutical alkaloid huperzine A (HupA), currently used in herbal supplements and medicines worldwide, is predominantly sourced from the Chinese lycopod Huperzia serrata (Thunb. ex Murray) Trev. (Lycopodiaceae), which on average contains only 0.08 mg HupA g(-1) dry weight, and is experiencing a rapid decline in China due to over-harvesting.
  
  
  OBJECTIVE
  To find a high-yielding, natural source of HupA and/or the related huperzine B (HupB) that could potentially be used as the starting material in a commercial propagation program.
  
  
  METHODS
  We surveyed 17 Huperzia species (15 indigenous to Australia and southeast Asia) for their foliar HupA and HupB concentrations. We also studied intra-specific variation for the huperzines in four species that were available in sufficient numbers, and determined tissue-specific accumulation in larger specimens.
  
  
  RESULTS
  HupA was detected in 11 Australasian and southeast Asian species, with eight also containing HupB, albeit at much lower concentrations. A H. elmeri (Herter) Holub plant from the Philippines had one of the highest HupA concentrations recorded (1.01 mg g(-1) dry wt) and it also had the highest HupB content of all plants surveyed (0.34 mg g(-1) dry wt). Intra-specific HupA and HupB concentrations were extremely variable, and at the intra-plant level, reproductive strobili were found to accumulate the highest HupA concentrations.
  
  
  CONCLUSIONS
  Select Huperzia species from Australia and southeast Asia have potential as the starting material for establishing commercial HupA plantations, but the high intra-specific variability observed suggests that detailed screening is needed to isolate high huperzine-yielding individuals.
authors: School of Botany, University of Melbourne, Australia.
authors: Wei-Han
authors: WH
authors: Lim
authors: Jason Q D
authors: JQ
authors: Goodger
authors: Ashley R
authors: AR
authors: Field
authors: Joseph A M
authors: JA
authors: Holtum
authors: Ian E
authors: IE
authors: Woodrow
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000600:supply & distribution; D001210:Asia, Southeastern; D044362:Australasia; D002800:Cholinesterase Inhibitors / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000600:supply & distribution; D002851:Chromatography, High Pressure Liquid; D032501:Huperzia / Q000737:chemistry*; D056626:Inflorescence / Q000737:chemistry; D007536:Isomerism; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000600:supply & distribution; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D012717:Sesquiterpenes; C050426:huperzine A; C053016:huperzine B