title: The moss Physcomitrella patens contains cyclopentenones but no jasmonates: mutations in allene oxide cyclase lead to reduced fertility and altered sporophyte morphology.
abstract: • Two cDNAs encoding allene oxide cyclases (PpAOC1, PpAOC2), key enzymes in the formation of jasmonic acid (JA) and its precursor (9S,13S)-12-oxo-phytodienoic acid (cis-(+)-OPDA), were isolated from the moss Physcomitrella patens. • Recombinant PpAOC1 and PpAOC2 show substrate specificity against the allene oxide derived from 13-hydroperoxy linolenic acid (13-HPOTE); PpAOC2 also shows substrate specificity against the allene oxide derived from 12-hydroperoxy arachidonic acid (12-HPETE). • In protonema and gametophores the occurrence of cis-(+)-OPDA, but neither JA nor the isoleucine conjugate of JA nor that of cis-(+)-OPDA was detected. • Targeted knockout mutants for PpAOC1 and for PpAOC2 were generated, while double mutants could not be obtained. The ΔPpAOC1 and ΔPpAOC2 mutants showed reduced fertility, aberrant sporophyte morphology and interrupted sporogenesis.
authors: Georg-August-University, Albrecht-von-Haller-Institute, Plant Biochemistry, Göttingen, Germany.
authors: Michael
authors: M
authors: Stumpe
authors: Cornelia
authors: C
authors: Göbel
authors: Bernd
authors: B
authors: Faltin
authors: Anna K
authors: AK
authors: Beike
authors: Bettina
authors: B
authors: Hause
authors: Kiyoshi
authors: K
authors: Himmelsbach
authors: Julia
authors: J
authors: Bode
authors: Robert
authors: R
authors: Kramell
authors: Claus
authors: C
authors: Wasternack
authors: Wolfgang
authors: W
authors: Frank
authors: Ralf
authors: R
authors: Reski
authors: Ivo
authors: I
authors: Feussner
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D019068:Bryopsida / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000302:isolation & purification; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism*; D051479:Plant Infertility / Q000235:genetics*; D011994:Recombinant Proteins; D013379:Substrate Specificity; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D011994:Recombinant Proteins; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; C011006:jasmonic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase; C013905:cyclopentenone