CTX-M-type extended-spectrum β-lactamases present in Escherichia coli from the feces of cattle in Ohio, United States.
CTX-M extended-spectrum β-lactamases are enzymes produced by bacteria that are capable of inhibiting the antimicrobial effects of cephalosporin drugs. Recently, the first domestically acquired Salmonella in the United States expressing bla(CTX-M) was reported. This is a concern because expanded-spectrum cephalosporins are the treatment of choice for invasive Gram-negative infections, including salmonellosis in children. Because Salmonella transmission is primarily foodborne, there is also concern that resistant enteric bacteria from livestock can be transferred through the food supply chain to consumers. bla(CTX-M) has not been previously identified in bacterial isolates from food animal populations in the United States. We report the recovery of CTX-M-type extended-spectrum β-lactamases from fecal Escherichia coli of sick and healthy dairy cattle in Ohio. Four individual fecal samples yielded E. coli isolates representing three clonal strains that carried bla(CTX-M) on transferable plasmids. Two distinguishable plasmids were identified, each encoding bla(CTX-M-1) or bla(CTX-M-79). Transferrable bla(CTX-M) genes in bovine E. coli have the potential to serve as a reservoir of resistance for pathogens and may represent a public health concern.
Wittum
Thomas E
TE
Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio 43210, USA. Thomas.Wittum@cvm.osu.edu
Mollenkopf
Dixie F
DF
Daniels
Joshua B
JB
Parkinson
Anne E
AE
Mathews
Jennifer L
JL
Fry
Pamela R
PR
Abley
Melanie J
MJ
Gebreyes
Wondwossen A
WA
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002511:Cephalosporins / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D005517:Foodborne Diseases / Q000382:microbiology; D005798:Genes, Bacterial; D019450:Genes, MDR; D058751:Livestock / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D009820:Ohio; D010957:Plasmids; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
D002511:Cephalosporins; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases