Nanofibers in a hyaluronan-based pericellular matrix.
The extracellular matrix of the brain is a highly organized hyaluronan-based supramolecular assembly that is involved in neuronal pathfinding, cell migration, synaptogenesis and neuronal plasticity. Here, we analyze the structure of the hyaluronan-rich pericellular matrix of an oligodendroglial precursor cell line using helium ion beam scanning microscopy at a subnanometer resolution. We find that thin nanofibers are the ultimate building elements of this oligodendroglial pericellular matrix. These structures may participate in the regulation of oligodendroglial maturation and motility.
Gerlach
Debora
D
Institut für Biochemie und Molekularbiologie, Universität Bonn, Nussallee 11, 53115 Bonn, Germany.
Kaminski
Tim
T
Pérez-Willard
Fabián
F
Kirfel
Gregor
G
Gieselmann
Volkmar
V
Kappler
Joachim
J
D000818:Animals; D002461:Cell Line, Transformed; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006820:Hyaluronic Acid / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics; D058580:Neurocan; D009836:Oligodendroglia / Q000648:ultrastructure*; D011509:Proteoglycans / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000648:ultrastructure
C507576:Ncan protein, mouse; D009419:Nerve Tissue Proteins; D058580:Neurocan; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006820:Hyaluronic Acid