title: ON-OFF switching of transcriptional activity of large DNA through a conformational transition in cooperation with phospholipid membrane.
abstract: We report that structural transitions of DNA cause the ON-OFF switching of transcriptional activity in cooperation with phospholipid membrane in a reconstituted artificial cell. It has been shown that long DNA of more than 20-30 kilo base-pairs exhibits a discrete conformational transition between a coiled state and highly folded states in aqueous solution, depending on the presence of various condensing agents such as polyamine. Recently, we reported a conformational transition of long DNA through interplay with phospholipid membrane, from a folded state in aqueous phase to an extended coil state on a membrane surface, in a cell-sized water-in-oil microdroplet covered by phosphatidylethanolamine monolayer (Kato, A.; Shindo, E.; Sakaue, T.; Tsuji, A.; Yoshikawa, K. Biophys. J. 2009, 97, 1678-1686). In this study, to elucidate the effects of these conformational changes on the biologically important function of DNA, transcription, we investigated the transcriptional activity of DNA in a microdroplet. Transcriptional activity was evaluated at individual DNA molecule level by a method we developed, in which mRNA molecules are labeled with fluorescent oligonucleotide probes. Transcription proceeded on almost all of the DNA molecules with a coiled conformation in the aqueous phase. In the presence of a tetravalent amine, spermine, the DNA had a folded conformation, and transcription was completely inhibited. When the Mg(2+) concentration was increased, DNA was adsorbed onto the inner surface of the membrane and exhibited an extended conformation. The transcription experiments showed that this conformational transition recovered transcriptional activity; transcription occurred on DNA molecules that were on the membrane.
authors: Spatio-Temporal Order, ICORP, Japan Science and Technology Agency, Tokyo 102-0075, Japan. tsuji@chem.scphys.kyoto-u.ac.jp
authors: Akihiko
authors: A
authors: Tsuji
authors: Kenichi
authors: K
authors: Yoshikawa
mesh_terms: D004247:DNA / Q000737:chemistry*; D006801:Humans; D009690:Nucleic Acid Conformation; D010743:Phospholipids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D010743:Phospholipids; D004247:DNA