title: [Establishment nephrolithiasis rat model induced by nanobacteria and analysis of stone formation].
abstract: OBJECTIVE
  To establish nephrolithiasis rat model induced by nanobacteria and to determine the relationship of nanobacteria with nephrolithiasis.
  
  
  METHODS
  Thirty adult male SD rats were randomized into 3 groups (n=10 each): group A, given an intravenous injection of nanobacteria; group B, given an intravenous injection of nanobacteria and taken tetracycline; group C, as normal control. Eight weeks later, all rats were sacrificed and the kidneys were examined for pathology and the 24-h urine N-acetyl-beta-D-aminoglycoside enzyme (NAG) and lactate dehydrogenase (LDH) were detected.
  
  
  RESULTS
  Histopathological studies revealed nanobacteria induced renal tubular crystallization, which was significantly different among the 3 groups (P=0.033). The crystallization was higher in group A than that in group B. The urinary NAG and LDH excretion were significantly different among the 3 groups (P<0.001).
  
  
  CONCLUSIONS
  Nanobacteria may be involved in the pathogenesis of nephrolithiasis. Tetracycline may inhibit stone formation.
authors: Department of Urology, Peking University People's Hospital, Beijing 100034, China.
authors: Wei-guo
authors: WG
authors: Hu
authors: Xiao-feng
authors: XF
authors: Wang
authors: Tao
authors: T
authors: Xu
authors: Jian-xing
authors: JX
authors: Li
authors: Liang
authors: L
authors: Chen
authors: Cheng-fan
authors: CF
authors: Yu
authors: Xiao-bo
authors: XB
authors: Huang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004195:Disease Models, Animal; D007669:Kidney Calculi / Q000382:microbiology*; D008297:Male; D053758:Nanoparticles; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley