title: Stability and robustness of blood variables in an antidoping context.
abstract: BACKGROUND
  With the setting up of the newly Athlete's Biological Passport antidoping programme, novel guidelines have been introduced to guarantee results beyond reproach. We investigated in this context, the effect of storage time on the variables commonly measured for the haematological passport. We also wanted to assess for these variables, the within and between analyzer variations.
  
  
  METHODS
  Blood samples were obtained from top level male professional cyclists (27 samples for the first part of the study and 102 for the second part) taking part to major stage races. After collection, they were transported under refrigerated conditions (2 °C < T < 12 °C), delivered to the antidoping laboratory, analysed and then stored at approximately 4 °C to conduct analysis at different time points up to 72 h after delivery. A mixed-model procedure was used to determine the stability of the different variables.
  
  
  RESULTS
  As expected haemoglobin concentration was not affected by storage and showed stability for at least 72 h. Under the conditions of our investigation, the reticulocytes percentage showed a much better stability than previous published data (> 48 h) and the technical comparison of the haematology analyzer demonstrated excellent results.
  
  
  CONCLUSIONS
  In conclusion, our data clearly demonstrate that as long as the World Anti-Doping Agency's guidelines are followed rigorously, all blood results reach the quality level required in the antidoping context.
authors: Laboratoire Suisse d'Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Epalinges, Switzerland. neil.robinson@chuv.ch
authors: N
authors: N
authors: Robinson
authors: P E
authors: PE
authors: Sottas
authors: T
authors: T
authors: Pottgiesser
authors: Y O
authors: YO
authors: Schumacher
authors: M
authors: M
authors: Saugy
mesh_terms: D056352:Athletes; D004300:Doping in Sports; D006403:Hematologic Tests / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results