Development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of beta-agonists in animal feed and drinking water.
A reproducible, sensitive and selective multiresidue analytical method for seven beta-agonists: clenbuterol (CBT), clenpenterol (CPT), ractopamine (RTP), brombuterol (BBT), mabuterol (MBT), mapenterol (MPT), and hydroxymethylclenbuterol (HMCBT) was developed and validated by using liquid chromatography tandem mass spectrometry (LC-MS/MS) in feed and drinking water samples. The validation was achieved according to the criteria laid down in the Commission Decision 2002/657/EC, however it was necessary to use minimum required performance limits (MRPLs) proposed by the Community Reference Laboratories (CRLs) due to the lack of maximum residue limits (MRLs) for beta-agonists. By setting up these MRPLs, allows controlling their use in safe mode, since beta-agonists are commonly used in veterinary medicine sometime in a fraudulent manner, for increasing the weigh of animals. Values set for both matrices studied are 50 microg/kg for animal feed, and a range from 0.2 to 10 microg/L for drinking water. CCalpha values calculated were under the MRPLs suggested; for drinking water the lowest value obtained was 0.12 microg/L, and for animal feed 0.87 microg/kg. Values for CCbeta were ranged from 0.08 to 0.13 microg/L in drinking water and from 0.5 to 0.92 microg/kg in animal feed samples. The excellence values obtained, allowed us to conclude that the proposed analytical method is capable to control the beta-agonists studied in both matrices and that it can be successfully applied and used as a routine method in laboratories of residue analysis of veterinary food control.
Juan
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Centro Superior de Investigación en Salud Pública, Unidad de Residuos de Medicamentos Veterinarios, Laboratorio de Salud Pública de Valencia, Generalitat Valenciana, Av. de Cataluña n degrees 21, 46020 València, Spain. juan_cri@gva.es
Igualada
C
C
Moragues
F
F
León
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N
Mañes
J
J
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004983:Ethanolamines / Q000032:analysis; D016014:Linear Models; D010627:Phenethylamines / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000737:chemistry; D014881:Water Supply / Q000032:analysis*
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D010627:Phenethylamines; D014867:Water; C060897:ractopamine