A weighted osteon morphotype score outperforms regional osteon percent prevalence calculations for interpreting cortical bone adaptation.
Using circularly polarized light microscopy,we described a weighted-scoring method for quantifying regional distributions of six secondary osteon morphotypes(Skedros et al.: Bone 44 (2009) 392-403). This osteon morphotype score (MTS) strongly correlated with "tension" and "compression" cortices produced by habitual bending. In the present study, we hypothesized that the osteon MTS is superior to a relatively simpler method based on the percent prevalence (PP) of these osteon morphotypes. This was tested in proximal femoral diaphyses of adult chimpanzees and habitually bent bones: calcanei from sheep, deer, and horses, radii from sheep and horses, and third metacarpals (MC3s) from horses. Sheep tibiae were examined because their comparatively greater torsion/shear would not require regional variations in osteon morphotypes. Predominant collagen fiber orientation (CFO), a predictor of regionally prevalent/predominant strain mode, was quantified as image gray levels (birefringence). Ten PP calculations were conducted. Although PP calculations were similar to the osteon MTS in corroborating CFO differences between "tension" and "compression" cortices of the chimpanzee femora and most of the habitually bent bones, PP calculations failed to show a compression/tension difference in equine MC3s and sheep radii. With the exception of the prevalence of the "distributed" osteon morphotype, correlations of PP calculations with CFO were weak and/or negative. By contrast, the osteon MTS consistently showed positive correlations with predominant CFO. Compared with the osteon MTS and predominant CFO, regional variations in PP of osteon morpho types are not stronger predictors of nonuniform strain distributions produced by bending.
Skedros
John G
JG
Bone and Joint Research Laboratory, Department of Veterans Affairs Medical Center, Salt Lake City, UT 84107, USA. jskedros@utahboneandjoint.com
Kiser
Casey J
CJ
Mendenhall
Shaun D
SD
D000222:Adaptation, Physiological; D000818:Animals; D001696:Biomechanical Phenomena; D002111:Calcaneus / Q000166:cytology; D003094:Collagen / Q000737:chemistry; D003670:Deer / Q000033:anatomy & histology; D018483:Diaphyses / Q000166:cytology; D006253:Haversian System / Q000166:cytology*; D006736:Horses / Q000033:anatomy & histology; D050279:Metacarpal Bones / Q000166:cytology; D008859:Microscopy, Polarization / Q000379:methods*; D002679:Pan troglodytes / Q000033:anatomy & histology; D011884:Radius / Q000166:cytology; D034561:Sheep, Domestic / Q000033:anatomy & histology; D013977:Tibia / Q000166:cytology; D016474:Weight-Bearing
D003094:Collagen