Comparison of in vitro acute cytotoxicity data obtained on human lymphocytes with known calculated human LD values of 20 selected drugs.
The acute in vitro cytotoxicity of 20 selected drugs at different concentrations was assayed biochemically by the leakage of DNA and lactate dehydrogenase from cells to medium. These two methods were supplemented with the MTT assay. The selected drugs had known estimated human LD values and had previously been tested in HeLa cells using morphological changes as an endpoint. The mitochondrial assay system usually gave smaller values than the other two methods. Spearman rank correlation analysis revealed correlation coefficients between the human estimated LD values and the LC(50) values found in the MTT, LDH and DNA assays of 0.68, 0.67 and 0.68, respectively.
Skaanild
M T
MT
Institute for Life Sciences and Chemistry, Roskilde University Centre, PO Box 260, DK-4000 Roskilde, Denmark.
Clausen
J
J