title: A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast.
abstract: To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.
authors: Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.
authors: Anna
authors: A
authors: Bink
authors: Gilmer
authors: G
authors: Govaert
authors: Isabelle E J A
authors: IE
authors: François
authors: Klaartje
authors: K
authors: Pellens
authors: Lieven
authors: L
authors: Meerpoel
authors: Marcel
authors: M
authors: Borgers
authors: Geert
authors: G
authors: Van Minnebruggen
authors: Valérie
authors: V
authors: Vroome
authors: Bruno P A
authors: BP
authors: Cammue
authors: Karin
authors: K
authors: Thevissen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000935:Antifungal Agents / Q000633:toxicity*; D017209:Apoptosis; D020558:GTP Phosphohydrolases / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects*; D024101:Mitochondrial Proteins / Q000378:metabolism; D010879:Piperazines / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000935:Antifungal Agents; C464859:FIS1 protein, S cerevisiae; C429283:MDV1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D010879:Piperazines; D029701:Saccharomyces cerevisiae Proteins; D020558:GTP Phosphohydrolases; C094774:DNM1 protein, S cerevisiae