Immunohistochemical localization of an N-acetyl amino-carbohydrate specific lectin (ACL-I) of the marine sponge Axinella corrugata.
The N-acetyl amino-carbohydrate specific lectin (ACL-I) was previously identified and purified by us from the marine sponge Axinella corrugata (phylum Porifera, class Demospongiae). The distribution of the specific lectin within the tissue of the sponge was studied by bright-field optical microscopy immunohistochemistry in order to better understand its physiological role in the sponge. Polyclonal antibodies were raised against purified ACL-I in mice and tested by Western blot technique. The immunohistochemical analysis of ACL-I in cross sections of A. corrugata showed that this lectin is found inside the denominated spherulous cells, which contain vesicles that store the lectin. Some evidence is shown that ACL-I might also be present in the extracellular matrix. It was not possible to demonstrate by the immunohistochemical technique if ACL-I is colocalized in both the plasma membrane and in the cytoplasm of the spherulous cells.
Dresch
Roger Remy
RR
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. rrdresch@gmail.com
Zanetti
Gilberto Dolejal
GD
Kanan
João Henrique Corrêa
JH
Mothes
Beatriz
B
Lerner
Cléa Beatriz
CB
Trindade
Vera Maria Treis
VM
Henriques
Amélia Teresinha
AT
Vozári-Hampe
Magdolna Maria
MM
D000818:Animals; D047089:Axinella / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology
D037102:Lectins