title: Outbreak of CTX-M-15-producing Klebsiella pneumoniae of sequence type 199 in a Latvian teaching hospital.
abstract: Previous studies on the epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in Latvia are lacking. ESBL-producing Klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (PFGE) and selected isolates to multi-locus sequence typing (MLST). Species identification and susceptibility testing were performed using VITEK2, and sequencing of bla(CTX-M) was performed in selected isolates. PFGE revealed one major clone (n = 23), with most of the isolates derived from the ICU. The clone harboured bla(CTX-M-15), was sequence type 199 and comprised two ertapenem non-susceptible isolates. This is the first report of an ESBL outbreak in Latvia, and calls for increased epidemiological typing of ESBL-producing Enterobacteriaceae, as well as improved infection control routines.
authors: Stradins Clinical University Hospital, Riga, Latvia.
authors: Uga
authors: U
authors: Dumpis
authors: Aina
authors: A
authors: Iversen
authors: Arta
authors: A
authors: Balode
authors: Māra
authors: M
authors: Saule
authors: Edvīns
authors: E
authors: Miklasevics
authors: Christian G
authors: CG
authors: Giske
mesh_terms: D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005060:Europe / Q000453:epidemiology; D020022:Genetic Predisposition to Disease; D006801:Humans; D007362:Intensive Care Units; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D007844:Latvia / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases