title: Gene transcription profiles, global DNA methylation and potential transgenerational epigenetic effects related to Zn exposure history in Daphnia magna.
abstract: A reduced level of DNA methylation has recently been described in both Zn-exposed and non-exposed offspring of Daphnia magna exposed to Zn. The hypothesis examined in this study is that DNA hypomethylation has an effect on gene transcription. A second hypothesis is that accumulative epigenetic effects can affect gene transcription in non-exposed offspring from parents with an exposure history of more than one generation. Transcriptional gene regulation was studied with a cDNA microarray. In the exposed and non-exposed hypomethylated daphnids, a large proportion of common genes were similarly up- or down-regulated, indicating a possible effect of the DNA hypomethylation. Two of these genes can be mechanistically involved in DNA methylation reduction. The similar transcriptional regulation of two and three genes in the F0 and F1 exposed daphnids on one hand and their non-exposed offspring on the other hand, could be the result of a one-generation temporary transgenerational epigenetic effect, which was not accumulative.
authors: Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J. Plateaustraat 22, B-9000 Ghent, Belgium. michiel.vandegehuchte@ugent.be
authors: Michiel B
authors: MB
authors: Vandegehuchte
authors: Dieter
authors: D
authors: De Coninck
authors: Tine
authors: T
authors: Vandenbrouck
authors: Wim M
authors: WM
authors: De Coen
authors: Colin R
authors: CR
authors: Janssen
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000187:drug effects; D003621:Daphnia / Q000187:drug effects* / Q000235:genetics; D044127:Epigenesis, Genetic / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D015032:Zinc