Enhanced gene delivery and siRNA silencing by gold nanoparticles coated with charge-reversal polyelectrolyte.
Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence of the charge-reversal property of functional gold nanoparticles. The expression efficiency of enhanced green fluorescent protein (EGFP) was improved by adjuvant transfection with charge-reversal functional gold nanoparticles, which also had much lower toxicity to cell proliferation. Lamin A/C, an important nuclear envelope protein, was effectively silenced by lamin A/C-siRNA delivered by charge-reversal functional gold nanoparticles, whose knockdown efficiency was better than that of commercial Lipofectamine 2000. Confocal laser scanning microscopic images indicated that there was more cy5-siRNA distributed throughout the cytoplasm for cyanine 5-siRNA/polyethyleneimine/cis-aconitic anhydride-functionalized poly(allylamine)/ polyethyleneimine/11-mercaptoundecanoic acid-gold nanoparticle (cy5-siRNA/PEI/PAH-Cit/PEI/MUA-AuNP) complexes. These results demonstrate the feasibility of using charge-reversal functional gold nanoparticles as a means of improving the nucleic acid delivery efficiency.
Guo
Shutao
S
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
Huang
Yuanyu
Y
Jiang
Qiao
Q
Sun
Yun
Y
Deng
Liandong
L
Liang
Zicai
Z
Du
Quan
Q
Xing
Jinfeng
J
Zhao
Yuliang
Y
Wang
Paul C
PC
Dong
Anjie
A
Liang
Xing-Jie
XJ
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014162:Transfection / Q000379:methods*
C505222:11-mercaptoundecanoic acid; D004337:Drug Carriers; D005227:Fatty Acids; D011073:Polyamines; D011108:Polymers; D034741:RNA, Small Interfering; D013438:Sulfhydryl Compounds; C063994:polyallylamine; D006046:Gold; D011094:Polyethyleneimine