title: The 126- and/or 183-kDa replicases or their coding regions are responsible both for inefficient local and for systemic movements of Paprika mild mottle virus Japanese strain in tomato plants.
abstract: Paprika mild mottle virus Japanese strain (PaMMV-J), a member of the genus Tobamovirus, was originally isolated from sweet pepper plants (Capsicum annuum L.). In experimental conditions, PaMMV-J spread more slowly in inoculated leaves of tomato plants and moved to uninoculated upper leaves at a lower frequency than Tomato mosaic virus (ToMV). In this study, we aimed to identify the viral factors responsible for the low efficiency of local and systemic movement of PaMMV-J in tomato plants. Using several viruses formed as chimeras between PaMMV-J and ToMV, we observed that a chimeric virus (Pa-RepL) having the 126- and 183-kDa replicase genes of ToMV could move systemically in tomato plants, similar to ToMV. Furthermore, analysis of a PaMMV-J mutant (PaMMV-1483C) showed that a single nucleotide substitution in 126- and 183-kDa replicase genes of PaMMV-J enhanced the efficiency of local movement of the virus in inoculated leaves to an extent similar to that of ToMV. However, PaMMV-1483C did not spread over the uninoculated upper leaves. In addition, viral RNA accumulation levels in tomato protoplasts inoculated with Pa-RepL and PaMMV-1483C were lower and similar to those of parental PaMMV-J. These results suggest that the 126- and/or 183-kDa replicases or their coding regions are responsible both for inefficient local and for systemic movements of PaMMV-J in tomato plants.
authors: Laboratory of Plant Pathology and Biotechnology, Kochi University, 200, Monobe, Nankoku, Kochi 783-8502, Japan.
authors: Hiroyuki
authors: H
authors: Mizumoto
authors: Kentaro
authors: K
authors: Kimura
authors: Akinori
authors: A
authors: Kiba
authors: Yasufumi
authors: Y
authors: Hikichi
mesh_terms: D019943:Amino Acid Substitution; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D017898:Tobamovirus / Q000201:enzymology* / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence
chemical_list: D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase