title: Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extracts.
abstract: Understanding of calcium signaling pathways in cells is essential for elucidating the mechanisms of both normal cell function and cancer development. Calcium messengers play the crucial role for intracellular Ca(2+) release. We propose a new approach to detecting the calcium second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) in cell extracts using surface-enhanced Raman spectroscopy (SERS). Currently available radioreceptor binding and enzymatic assays require extensive sample preparation and take more than 12 h. With a SERS sensor, NAADP can be detected in less than 1 min without any special sample preparation. To the best of our knowledge, this is the first demonstration of using SERS for calcium signaling applications.
authors: Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA.
authors: Elina A
authors: EA
authors: Vitol
authors: Eugen
authors: E
authors: Brailoiu
authors: Zulfiya
authors: Z
authors: Orynbayeva
authors: Nae J
authors: NJ
authors: Dun
authors: Gary
authors: G
authors: Friedman
authors: Yury
authors: Y
authors: Gogotsi
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D045744:Cell Line, Tumor; D036563:Cyclic ADP-Ribose / Q000737:chemistry / Q000502:physiology; D006046:Gold / Q000737:chemistry; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000737:chemistry / Q000502:physiology; D053768:Metal Nanoparticles / Q000737:chemistry; D009249:NADP / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000502:physiology; D015290:Second Messenger Systems; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D036563:Cyclic ADP-Ribose; D009249:NADP; C024376:NAADP; D006046:Gold; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium