Reactions of medicinally relevant gold compounds with the C-terminal motif of thioredoxin reductase elucidated by MS analysis.
The tetrapeptide Ac-Gly-[Cys-Sec]-Gly-NH(2), reproducing the C-terminal motif of the selenoenzyme thioredoxin reductase, was designed and synthesized, and its reactions with a few medicinally relevant gold(i,iii) compounds investigated by ESI-MS. Remarkably, the main reaction products could be unambiguously identified providing valuable insight into the likely mechanisms of enzyme inhibition by gold compounds.
Pratesi
Alessandro
A
Laboratory of Peptide & Protein Chemistry & Biology, Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 13, 50019 Sesto Fiorentino, Firenze, Italy.
Gabbiani
Chiara
C
Ginanneschi
Mauro
M
Messori
Luigi
L
D017612:Gold Compounds / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry / Q000378:metabolism*
D017612:Gold Compounds; D009842:Oligopeptides; D013880:Thioredoxin-Disulfide Reductase