Prostate secretions from men with chronic pelvic pain syndrome inhibit proinflammatory mediators.
OBJECTIVE
In the past numerous chemokines have been noted in the expressed prostatic secretions of patients with chronic prostatitis/chronic pelvic pain syndrome. We examined the functional effects of chemokines in expressed prostatic secretions of patients with chronic pelvic pain syndrome.


METHODS
We studied the functional effects of expressed prostatic secretions on human monocytes by examining monocyte chemotaxis in response to monocyte chemoattractant protein-1, a major chemoattractant previously identified in chronic prostatitis/chronic pelvic pain syndrome cases. We determined effects on cellular signaling by quantifying intracellular calcium increase in monocytes and nuclear factor-κB activation in normal prostate epithelial cells.


RESULTS
Results show that the monocyte chemoattractant protein-1 in expressed prostatic secretions is nonfunctional with an inability to mediate human monocyte chemotaxis, or mediate signaling in monocytes or prostate epithelial cells. This lack of functionality could be extended to other proinflammatory cytokines, such as interleukin-1β and tumor necrosis factor-α, when incubated with expressed prostatic secretions from patients with chronic pelvic pain syndrome. The mechanism underlying this apparent ability to modulate proinflammatory cytokines involves heat labile extracellular proteases that mediate the inhibition of immune and prostate epithelial cell function.


CONCLUSIONS
These results may have implications for the design of specific diagnostic and therapeutic methods targeted toward the complete resolution of prostate inflammatory insults.
Thumbikat
Praveen
P
Department of Urology and Division of Rheumatology, Northwestern University, Chicago, Illinois 60611, USA. thumbikat@northwestern.edu
Shahrara
Shiva
S
Sobkoviak
Rudina
R
Done
Joseph
J
Pope
Richard M
RM
Schaeffer
Anthony J
AJ
D018932:Chemokine CCL2 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011472:Prostatitis / Q000276:immunology*
D018932:Chemokine CCL2; D018836:Inflammation Mediators