title: Genotypic variation of Pneumocystis jirovecii isolates in India based on sequence diversity at mitochondrial large subunit rRNA.
abstract: Pneumocystis pneumonia (PCP), a common and serious opportunistic infection in immunocompromised patients, is caused by Pneumocystis jirovecii (formerly known as Pneumocystis carinii f. sp. hominis). The aim of the present study was to describe the prevalence and distribution of genotypes of P. jirovecii based on sequence polymorphisms at mitochondrial large subunit ribosomal RNA (mt LSU rRNA) region in both HIV and non-HIV immunocompromised individuals with a positive PCR result for PCP in a tertiary health care centre in northern India. From January 2005 to October 2008, 50 patients [22 HIV-seropositive individuals, 10 post-renal transplant (PRT) recipients, 3 cancer patients, and 15 patients with various other kinds of immunosuppression] were found to be positive for P. jirovecii using PCR at the mt LSU rRNA gene. Genotyping of the positive samples was performed at the mt LSU rRNA locus. Genotype 2 was the most common accounting for 42% of total types. This was followed by the genotypes 3 (24%), 1 (20%), and 4 (8%). Mixed infection was observed in 3 cases (6%). The rates of genotype distribution were similar in HIV-seropositive individuals, cancer patients, and in patients with other kinds of immunosuppression. In the PRT recipients, genotype 1 was the most prevalent type (80%). This is the first study describing the prevalence of genotypes in HIV-infected and HIV-uninfected, immunocompromised patients based on the mt LSU rRNA gene from the Indian subcontinent. The most prevalent genotype observed was type 2 in contrast to many studies from other parts of the world where genotype 1 was the most prevalent type, suggesting geographical variation.
authors: Dept. of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
authors: Rashmi
authors: R
authors: Gupta
authors: Bijay Ranjan
authors: BR
authors: Mirdha
authors: Randeep
authors: R
authors: Guleria
authors: Sanjay Kumar
authors: SK
authors: Agarwal
authors: Jyotish Chandra
authors: JC
authors: Samantaray
authors: Lalit
authors: L
authors: Kumar
authors: Sushil Kumar
authors: SK
authors: Kabra
authors: Kalpana
authors: K
authors: Luthra
authors: Vishnubhatla
authors: V
authors: Sreenivas
authors: Venkateswaran K
authors: VK
authors: Iyer
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016867:Immunocompromised Host; D007194:India; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D045363:Pneumocystis carinii / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S