Multivalent ions control the transport through lysenin channels.
We report the effect of different ions on the conducting properties of lysenin channels inserted into planar lipid bilayer membranes. Our observations indicated that multivalent ions inhibited the lysenin channels conductance in a concentration dependent manner. The analysis performed on single channels revealed that multivalent ions induced reversible sub-conducting or closed states depending on the ionic charge and size. Good agreement is reported between experimental results and a theoretical model that is proposed to describe the interaction between divalent ions and lysenin channels as a simple isothermal absorption process.
Fologea
Daniel
D
Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA. dfologea@uark.edu
Krueger
Eric
E
Al Faori
Redwan
R
Lee
Rachel
R
Mazur
Yuriy I
YI
Henry
Ralph
R
Arnold
Mark
M
Salamo
Greg J
GJ
D000042:Absorption; D017136:Ion Transport; D007477:Ions / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D014118:Toxins, Biological / Q000737:chemistry* / Q000502:physiology
D007477:Ions; D008051:Lipid Bilayers; D008670:Metals; D014118:Toxins, Biological; C107040:lysenin