Identification of transcripts up-regulated in asexual and sexual fruiting bodies of the Dutch elm disease pathogen Ophiostoma novo-ulmi.
Suppression subtractive hybridization cDNA libraries were prepared from asexual synnemata (S-lib) and sexual perithecia (P-lib) fruiting bodies of the Dutch elm disease pathogen Ophiostoma novo-ulmi subsp. novo-ulmi isolate H327 (mating-type MAT1-1) consisting of 630 and 401 cDNA clones, respectively. Both libraries were differentially screened in duplicate with forward and reverse subtracted probes. Up-regulated S-lib transcripts included those with homologies to phosphoenolpyruvate carboxykinase and aquaporin. Up-regulated P-lib transcripts included those with homologies to aspartyl proteinase, DNA lyase 2, and part of a mating-type (MAT) protein containing a DNA-binding domain of the high-mobility group (HMG) type. Phylogenetic analyses of HMG domains present within the putative O. novo-ulmi MAT protein and within MAT1-1-3 and MAT1-2-1 proteins of other ascomycete fungi identified the O. novo-ulmi protein as a homologue of the MAT1-1-3 protein, which represents part of the so far uncharacterized O. novo-ulmi MAT1-1 idiomorph. Reverse transcription - quantitative real-time PCR indicated up-regulation of the MAT1-1-3 homologue in O. novo-ulmi perithecia and synnemata. The present work identifies, for the first time, proteins involved in the formation of asexual and sexual fruiting bodies in O. novo-ulmi and should be of interest to researchers concerned with reproduction, mating type, and sexuality of filamentous ascomycete fungi.
Jacobi
Volker
V
Centre d'étude de la forêt (CEF) and Institut de biologie intégrative et des systèmes (IBIS), Université Laval, Pavillon Charles-Eugène Marchand, 1030, avenue de la Médecine, Québec, QC G1V 0A6, Canada. Volker.Jacobi@sbf.ulaval.ca
Dufour
Josée
J
Bouvet
Guillaume F
GF
Aoun
Mirella
M
Bernier
Louis
L
D003001:Cloning, Molecular; D048690:Fruiting Bodies, Fungal / Q000254:growth & development* / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism*; D015723:Gene Library; D049770:Genes, Mating Type, Fungal; D024742:HMG-Box Domains; D055156:Ophiostoma / Q000472:pathogenicity* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D005656:Fungal Proteins