title: Localization of nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis.
abstract: The nephritis-associated plasmin receptor is a recently identified nephritogenic antigen associated with acute poststreptococcal glomerulonephritis and proposed to play a pathogenic role, but its precise glomerular localization in acute poststreptococcal glomerulonephritis has not been elucidated. We therefore analyzed renal biopsy sections from 10 acute poststreptococcal glomerulonephritis patients by using immunofluorescence staining with anti-nephritis-associated plasmin receptor antibody and various markers of glomerular components. Nephritis-associated plasmin receptor was detected in the glomeruli of all patients, and double staining for nephritis-associated plasmin receptor and collagen IV showed nephritis-associated plasmin receptor to be predominantly on the inner side of the glomerular tufts. Nephritis-associated plasmin receptor-positive areas within glomerular tufts were further characterized with markers for neutrophils, mesangial cells, endothelial cells, and macrophages. In 6 of the patients, nephritis-associated plasmin receptor staining was seen mainly in neutrophils and to a lesser degree in mesangial and endothelial cells. In the other 4 patients, nephritis-associated plasmin receptor staining was seen mainly in mesangial cells and to a lesser degree in neutrophils and endothelial cells. In all patients, macrophages showed little staining. Elevated plasmin activity in glomerular neutrophils was identified by combining in situ zymography staining for plasmin activity and immunofluorescence staining for neutrophils. The glomerular localizations of nephritis-associated plasmin receptor and another nephritogenic antigen, streptococcal pyrogenic exotoxin B, were compared by double immunofluorescence staining and found to be similar. These findings indicate the nephritogenic potential of nephritis-associated plasmin receptor and offer valuable information with respect to the pathogenic mechanism of acute poststreptococcal glomerulonephritis.
authors: Department of Internal Medicine, National Defense Medical College, Saitama 359-8513, Japan. takashio@ndmc.ac.jp
authors: Takashi
authors: T
authors: Oda
authors: Nobuyuki
authors: N
authors: Yoshizawa
authors: Kazuo
authors: K
authors: Yamakami
authors: Kikuko
authors: K
authors: Tamura
authors: Aki
authors: A
authors: Kuroki
authors: Tetsuzo
authors: T
authors: Sugisaki
authors: Emi
authors: E
authors: Sawanobori
authors: Kohsuke
authors: K
authors: Higashida
authors: Yoshiyuki
authors: Y
authors: Ohtomo
authors: Osamu
authors: O
authors: Hotta
authors: Hiroo
authors: H
authors: Kumagai
authors: Soichiro
authors: S
authors: Miura
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D005098:Exotoxins / Q000378:metabolism; D005260:Female; D005920:Glomerular Mesangium / Q000378:metabolism / Q000473:pathology; D005921:Glomerulonephritis / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013290:Streptococcal Infections / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D005098:Exotoxins; D011956:Receptors, Cell Surface; C027951:erythrogenic toxin; C517776:nephritis-associated plasminogen receptor, Streptococcus