title: Glycan characterization of PSA 2-DE subforms from serum and seminal plasma.
abstract: Prostate-specific antigen (PSA) two-dimensional electrophoresis (2-DE) subforms (F1-F5) have been described to be altered in prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH). To understand their molecular differences, characterization of these subforms from PCa serum and seminal plasma, namely, at the glycan level, was performed. PSA 2-DE subforms from two serum PCa samples and seminal plasma were analyzed by N-glycan sequencing using high-performance liquid chromatography (HPLC) combined with exoglycosidase array digestions and by mass spectrometry. F1, F2, and F3 subforms showed the same N-glycan pattern, which contained higher levels of sialic acid than the F4 subform, whereas the F5 subform was unglycosylated. When comparing PSA subforms from PCa with seminal plasma, a decrease in sialylation was observed. Furthermore, the analysis of F3, the more abundant PSA subform, showed a higher proportion of alpha 2-3 sialic acid and a decrease in core fucosylated glycans in the PCa sample. These N-glycan changes in PCa PSA subforms highlight the importance of glycosylation as an indicator of PCa disease.
authors: Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Girona, Spain.
authors: Ariadna
authors: A
authors: Sarrats
authors: Radka
authors: R
authors: Saldova
authors: Josep
authors: J
authors: Comet
authors: Niaobh
authors: N
authors: O'Donoghue
authors: Rafael
authors: R
authors: de Llorens
authors: Pauline M
authors: PM
authors: Rudd
authors: Rosa
authors: R
authors: Peracaula
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D006031:Glycosylation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis*; D017430:Prostate-Specific Antigen / Q000097:blood / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000097:blood / Q000737:chemistry; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry; D012661:Semen / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides; D020033:Protein Isoforms; D017430:Prostate-Specific Antigen