[Effects of Rap2b gene on foci formation and wound-healing of NIH3T3 cells].
OBJECTIVE
To explore the effects on foci formation and wound-healing of NIH3T3 cells, and to provid the experimental evidence of its function.


METHODS
DNA from human lung cells was extracted and amplification of Rap2b gene was done by PCR. Eukaryotic expression vector pcDNA3. 1-Rap2b was constructed and was stable transfected into NIH3T3 cell followed with foci formation assay and wound-healing assay.


RESULTS
The number of the foci formation of NIH3T3 cell transfected by eukaryotic expression vector pcDNA3. 1-Rap2b was increased remarkably in foci formation assay (P < 0.01) and NIH3T3 cells transfected by eukaryotic expression vector pcDNA3. 1-Rap2b were quickly heal up in wound-healing assay.


CONCLUSIONS
The extrinsic expression of Rap2b could transform NIH3T3 cell using foci formation assay and wound-healing assay. Rap2b gene might play an oncogenic role in tumorigenesis.
Zhang
Qiao
Q
Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. zhangqiao@zzu.edu.cn
Duan
Liju
L
Xu
Honghui
H
Yuan
Jinsong
J
Wu
Weidong
W
Wu
Yiming
Y
Gao
Yanning
Y
Cheng
Shujun
S
D000818:Animals; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D020668:rap GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D004247:DNA; C065562:RAP2B protein, human; D020668:rap GTP-Binding Proteins