title: Selective recognition and elimination of nicotinic acetylcholine receptor-reactive B cells by a recombinant fusion protein AChR-Fc in myasthenia gravis in vitro.
abstract: AChR-reactive B cells play a key role in the pathogenesis of myasthenia gravis (MG) by producing autoantibodies. Selective elimination of AChR-reactive B cells will be a promising way to treat MG. Thus, we generated a fusion protein (referred to as AChR-Fc) composed of the human extracellular domain of AChR α1 subunit and the Fc domain of the human IgG1 heavy chain, which could bind both to AChR-reactive BCR and FcγRIIB on the surface of AChR-reactive B cells. Our results showed that AChR-Fc inhibited the proliferation of AChR-specific hybridoma cells, promoted their apoptosis, and mediated cytotoxicity by cross-linking effector cells and complement. Likewise, AChR-Fc significantly reduced the number of AChR-reactive B cells from spleen of Lewis rats immunized with AChR ex vivo.
authors: Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710038, China.
authors: Ting
authors: T
authors: Chang
authors: Hong
authors: H
authors: Lin
authors: Jie
authors: J
authors: Gao
authors: Wei
authors: W
authors: Li
authors: Jiang
authors: J
authors: Xu
authors: Chen Jing
authors: CJ
authors: Sun
authors: Hang
authors: H
authors: Li
authors: Fan Fan
authors: FF
authors: Li
authors: Yue
authors: Y
authors: Song
authors: Jing
authors: J
authors: Ye
authors: Zhu Yi
authors: ZY
authors: Li
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D006825:Hybridomas; D008212:Lymphocyte Depletion / Q000379:methods; D009157:Myasthenia Gravis / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D051381:Rats; D011917:Rats, Inbred Lew; D017452:Receptors, IgG / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis* / Q000378:metabolism / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
chemical_list: C499173:FCGR2B protein, human; D017452:Receptors, IgG; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins