title: Reversible circularization of an anthracene-modified DNA conjugate through bimolecular triplex formation and its analytical application.
abstract: We prepared an oligodeoxyribonucleotide conjugate (5-3ant(2)18) carrying two anthracenes, each of which was tethered to both ends of the conjugate through hexamethylene linker chains. The conjugate has a mirror repeat of two heptamer sequences, such that it forms a bimolecular triplex with the single stranded target, forming a two-fold U-shaped conformation. The conformation of the conjugate in its triplex structure could be frozen instantaneously by circularization through photodimerization of the anthracenes. Compared with the duplex formation of linear probes with relevant sequences, bimolecular triplex formation of 5-3ant(2)18 shows a unique feature in its target recognition; it binds the target tightly, yet still retains high sequence selectivity. Circularization of 5-3ant(2)18 by UV photoirradiation was verified as the probe reaction for a DNA assay. The probe reaction could be performed in a few seconds over a wide range of temperatures, at least between 0 and 25 °C. In addition, the reaction could be regarded as a reversible method for the preparation of circular DNA that shows higher affinity for the target.
authors: Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
authors: Pelin
authors: P
authors: Arslan
authors: Akinori
authors: A
authors: Jyo
authors: Toshihiro
authors: T
authors: Ihara
mesh_terms: D000873:Anthracenes / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry*; D019281:Dimerization; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D055668:Photochemical Processes; D014466:Ultraviolet Rays
chemical_list: D000873:Anthracenes; D004270:DNA, Circular; D009838:Oligodeoxyribonucleotides; D004247:DNA; C034020:anthracene