title: Biomaterials modulate interleukin-8 and other inflammatory proteins during reepithelialization in cutaneous partial-thickness wounds in pigs.
abstract: Acute and chronic cutaneous wounds remain a clinical challenge that require a mechanistic understanding to advance treatment options. For example, the role of inflammatory mediators during wound healing is not completely understood. Biomimetic materials, such as an in situ photopolymerizable semi-interpenetrating network (sIPN) derived from extracellular matrix components, show great potential in improving healing through the delivery of therapeutic agents and the function as a temporary tissue scaffold. In this study, we characterized the temporal profile of porcine cutaneous partial-thickness wound healing in response to Xeroform and sIPN treatment via histological and inflammatory protein analyses in epidermal, remodeling dermal, and dermal regions. Generally, interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, interferon-γ, and tumor necrosis factor-α, but not IL-8, were expressed in the epidermis and remodeling dermis in a time course that followed the progression of epidermal maturation in response to both treatments. Differences in cellularity and protein expression were observed between treatments in a time- and region-dependent manner. In particular, the healing response to sIPN exemplified a potentially key relationship between IL-8 expression and reepithelialization. These results provide insights into the expression of inflammatory mediators and the time course of cutaneous healing and the capacity for biomaterials to further modulate this relationship.
authors: Pharmaceutical Sciences Division, School of Pharmacy, College of Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors: Kyle R
authors: KR
authors: Kleinbeck
authors: Lee D
authors: LD
authors: Faucher
authors: Weiyuan J
authors: WJ
authors: Kao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis / Q000378:metabolism / Q000473:pathology; D005500:Follow-Up Studies; D016209:Interleukin-8 / Q000378:metabolism*; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013552:Swine; D014945:Wound Healing; D014947:Wounds and Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D016209:Interleukin-8