[Effects of total alkaloid of Sophora alopecuroides on serum IL-1beta and IL-4 expression in mice with acute ulcerative colitis].
OBJECTIVE
To investigate the effects of total alkaloid of Sophora alopecuroides (TASA) on expression of serum IL-1beta and IL-4 in mice with DSS-induced acute ulcerative colitis.


METHODS
C57BL/C mice were randomly divided into six groups: normal group, model group, SASP group, low, middle and high dosage TASA group. Acute ulcerative colitis was induced by administrating 5% dextran sulfate sodium (DSS) solution as drinking water freely for 8 days. Mice were given drug by stomach at the same time and sacrificed after 8 days. The general morphological and histopathological changes were observed during the experiments. Daily disease activity index (DAI) was assessed. The serum levels of IL-1beta and IL-4 were detected by ELISA.


RESULTS
In UC mice, the expression of serum IL-1beta was significantly increased whereas IL-4 was decreased when compared with the normal group (P < 0.01). In all treatment groups, TASA could reduce the expression of IL-1beta (P < 0. 01) , increase the level of IL-4 and alleviate the symptom of UC and decrease the DAI.


CONCLUSIONS
TASA can effectively attenuate DSS-induced acute ulcerative colitis through the decreasing the over-expression of pro-inflammatory cytokine levels.
Wang
Xiaojuan
X
College of Traditional Chinese Medicine and Pharmacy, Southern Medical University, Guangzhou 510515, China.
Jiang
Bin
B
Li
Lijun
L
Yao
Hui
H
Deng
Hongzhu
H
D000470:Alkaloids / Q000008:administration & dosage*; D000818:Animals; D003093:Colitis, Ulcerative / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics* / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000008:administration & dosage*; D011897:Random Allocation; D029909:Sophora / Q000737:chemistry*
D000470:Alkaloids; D053583:Interleukin-1beta; D010936:Plant Extracts; D015847:Interleukin-4