title: A pseudo-plaque method for infectious particle assay and clonal isolation of adeno-associated virus.
abstract: A colorimetric method has been developed for the detection of adeno-associated virus (AAV) infectious centers in cell culture monolayers. Due to its non-cytopathic nature, AAV has not been amenable to the traditional plaque assay, involving an agar overlay and cellular stains. As a result, an alternate method was required. The pseudo-plaque assay is based on enzyme-catalyzed color development after a fixed cell monolayer is probed with anti-AAV monoclonal antibodies. In spite of chemical fixation, expected to damage the viral genomes and particles, infectious particles can be recovered and amplified for the propagation of viral clones.
authors: Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306-4380, USA.
authors: Daniel A J
authors: DA
authors: Mitchell
authors: Thomas F
authors: TF
authors: Lerch
authors: Joan T
authors: JT
authors: Hare
authors: Michael S
authors: MS
authors: Chapman
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003124:Colorimetry; D000229:Dependovirus / Q000302:isolation & purification* / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D019562:Viral Load; D010948:Viral Plaque Assay / Q000379:methods*; D014771:Virion / Q000254:growth & development / Q000302:isolation & purification; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal