Targeting P2X₇ receptor inhibits the metastasis of murine P388D1 lymphoid neoplasm cells to lymph nodes.
The P2X₇R (P2X₇ receptor) is an ATP-gated cation channel expressed in normal cells that participates in both cell proliferation and apoptosis. Here, we have confirmed P2X₇R expression on murine P388D1 lymphoid neoplasm cells. In addition, ATP-stimulated P2X₇R expression was found to trigger increased intracellular calcium flux. Furthermore, silencing with short hairpin RNA and blocking with P2X₇R antibody significantly reduced the metastasis of P388D1 cells to lymph nodes. These results indicate that inhibition of the expression and function of P2X₇R attenuates the metastatic ability of murine lymphoid neoplasm cell line P388D1, which represents a new potential target for anti-metastatic therapy.
Ren
Shuangyi
S
Department of Surgery, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Peoples Republic of China.
Zhang
Yi
Y
Wang
Yujing
Y
Lui
Yuejian
Y
Wei
Wei
W
Huang
Xiaohua
X
Mao
Wenjuan
W
Zuo
Yunfei
Y
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D045744:Cell Line, Tumor; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D008207:Lymphatic Metastasis; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008223:Lymphoma / Q000235:genetics / Q000473:pathology* / Q000517:prevention & control*; D051379:Mice; D008811:Mice, Inbred DBA; D058990:Molecular Targeted Therapy; D009368:Neoplasm Transplantation; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology / Q000627:therapeutic use*; D034741:RNA, Small Interfering / Q000494:pharmacology / Q000627:therapeutic use; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism / Q000502:physiology
D058920:Purinergic P2X Receptor Antagonists; D034741:RNA, Small Interfering; D058486:Receptors, Purinergic P2X7; C063302:KN 62; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine