[Study on sensitization and mechanism of CGA-BSA].
OBJECTIVE
To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).


METHODS
Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.


RESULTS
There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.


CONCLUSIONS
Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.
Huang
Xiaowu
X
Clinical Pharmacology Laboratory, PLA General Hospital, Beijing 100853, China.
Liao
Hongbo
H
Liu
Ping
P
Lin
Hui
H
Yuan
Liyan
L
Hu
Yuan
Y
D000818:Animals; D002726:Chlorogenic Acid / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects*; D006168:Guinea Pigs; D006636:Histamine Release; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D008407:Mast Cells / Q000276:immunology; D011897:Random Allocation; D012710:Serum Albumin, Bovine / Q000276:immunology*
D004365:Drugs, Chinese Herbal; D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D007073:Immunoglobulin E