title: A complementary role for the tetraspanins CD37 and Tssc6 in cellular immunity.
abstract: The cooperative nature of tetraspanin-tetraspanin interactions in membrane organization suggests functional overlap is likely to be important in tetraspanin biology. Previous functional studies of the tetraspanins CD37 and Tssc6 in the immune system found that both CD37 and Tssc6 regulate T cell proliferative responses in vitro. CD37(-/-) mice also displayed a hyper-stimulatory dendritic cell phenotype and dysregulated humoral responses. In this study, we characterize "double knockout" mice (CD37(-/-)Tssc6(-/-)) generated to investigate functional overlap between these tetraspanins. Strong evidence for a cooperative role for these two proteins was identified in cellular immunity, where both in vitro T cell proliferative responses and dendritic cell stimulation capacity are significantly exaggerated in CD37(-/-)Tssc6(-/-) mice when compared with single knockout counterparts. Despite these exaggerated cellular responses in vitro, CD37(-/-)Tssc6(-/-) mice are not more susceptible to autoimmune induction. However, in vivo responses to pathogens appear poor in CD37(-/-)Tssc6(-/-) mice, which showed a reduced ability to produce influenza-specific T cells and displayed a rapid onset hyper-parasitemia when infected with Plasmodium yoelii. Therefore, in the absence of both CD37 and Tssc6, immune function is further altered when compared with CD37(-/-) or Tssc6(-/-) mice, demonstrating a complementary role for these two molecules in cellular immunity.
authors: Department of Immunology, Monash University, Melbourne, Victoria, Australia.
authors: Kate H
authors: KH
authors: Gartlan
authors: Gabrielle T
authors: GT
authors: Belz
authors: Jacqueline M
authors: JM
authors: Tarrant
authors: Gabriela
authors: G
authors: Minigo
authors: Maria
authors: M
authors: Katsara
authors: Kuo-Ching
authors: KC
authors: Sheng
authors: Mariam
authors: M
authors: Sofi
authors: Annemiek B
authors: AB
authors: van Spriel
authors: Vasso
authors: V
authors: Apostolopoulos
authors: Magdalena
authors: M
authors: Plebanski
authors: Lorraine
authors: L
authors: Robb
authors: Mark D
authors: MD
authors: Wright
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000502:physiology*; D001169:Arthritis, Experimental / Q000235:genetics / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D016130:Immunophenotyping; D007251:Influenza, Human / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008288:Malaria / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D059470:Tetraspanins
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C059071:CD37 protein, human; D008565:Membrane Proteins; C485950:TSPAN32 protein, human; D059470:Tetraspanins