Relation of MT1-MMP gene expression to outcomes in colorectal cancer.
BACKGROUND
Matrix metalloproteinases are members of a large family of endopeptidases that participate in the extracellular-matrix degradation that accompanies cancer cell invasion, metastasis and angiogenesis. The membrane-type 1 matrix metalloproteinase (MT1-MMP) gene has been reported in various cancers and is associated with tumor invasion and metastasis. This study examined the relation of the relative expression of MT1-MMP gene to clinicopathological factors and outcomes in patients with colorectal cancer (CRC).


METHODS
We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated CRC. The relative expression levels of MT1-MMP mRNA in cancer and in normal adjacent mucosa were measured by quantitative real-time reverse-transcriptase polymerase chain reaction.


RESULTS
MT1-MMP gene expression was higher in cancer tissue than in adjacent normal mucosa. The level of MT1-MMP gene expression was not related to any clinicopathological factor. Overall survival at 5 years differed significantly between patients with high MT1-MMP gene expression and those with low expression.


CONCLUSIONS
Overexpression of the MT1-MMP gene is considered a useful independent predictor of outcomes in patients with CRC.
Kanazawa
Amane
A
Department of Gastrointestinal Surgery, Kanagawa Cancer Center, Kanagawa-ken, Japan. amanex2009@gmail.com
Oshima
Takashi
T
Yoshihara
Kazue
K
Tamura
Shuzo
S
Yamada
Takanobu
T
Inagaki
Daisuke
D
Sato
Tsutomu
T
Yamamoto
Naoto
N
Shiozawa
Manabu
M
Morinaga
Soichiro
S
Akaike
Makoto
M
Kunisaki
Chikara
C
Tanaka
Katsuaki
K
Masuda
Munetaka
M
Imada
Toshio
T
D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D016896:Treatment Outcome
D012333:RNA, Messenger; D053511:Matrix Metalloproteinase 14