Stability comparison between sample preparation procedures for mass spectrometry-based targeted or shotgun peptidomic analysis.
The quantification of neuropeptides may play a significant role in future drug development targeting central nervous system functions. Adequate method precision and accuracy is essential, and sample stability is an important factor. This study compares three sample preparation protocols and assesses the stability of targeted neuropeptides under standard laboratory conditions. The results show that the concentrations of substance P, dynorphin A, and calcitonin gene-related peptide (CGRP) change significantly in time when spinal cord tissues are homogenized in phosphate-buffered saline (PBS) buffer or PBS buffer containing a mammalian protease inhibitor cocktail but is stabilized when tissues are homogenized in a 0.25% trifluoroacetic acid solution.
Beaudry
Francis
F
Groupe de recherche en pharmacologie animal du Québec (GREPAQ), Département de biomédecine vétérinaire, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 2M2. francis.beaudry@umontreal.ca
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000737:chemistry*; D004399:Dynorphins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D055550:Protein Stability; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism; D013373:Substance P / Q000737:chemistry*; D013696:Temperature
D013373:Substance P; D004399:Dynorphins; D015740:Calcitonin Gene-Related Peptide