title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2.
abstract: Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of 1,011 bp, 1,008 bp and 1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A: 3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (> 90%) of homology. Furthermore, the plpE gene of Haemorhhagic septicaemia causing serotype (B: 2) was expressed in E. coli and recombinant PlpE was strongly immunostained by antiserum against whole cell antigen, indicating that the protein is expressed in vivo.
authors: Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly- 243122, UP, India.
authors: Ajay Pratap
authors: AP
authors: Singh
authors: Satparkash
authors: S
authors: Singh
authors: Rajeev
authors: R
authors: Ranjan
authors: Santosh Kumar
authors: SK
authors: Gupta
authors: Vijendra Pal
authors: VP
authors: Singh
authors: Bhaskar
authors: B
authors: Sharma
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D014644:Genetic Variation; D006483:Hemorrhagic Septicemia / Q000382:microbiology / Q000662:veterinary*; D007194:India; D008074:Lipoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016979:Pasteurella multocida / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012703:Serotyping; D013045:Species Specificity
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins