Investigation of changes in skeletal muscle alpha-actin expression in normal and pathological human and mouse hearts.
We have developed a quantitative antibody-based assay to measure the content of skeletal muscle alpha-actin relative to cardiac alpha-actin. We found 21 +/- 2% skeletal muscle alpha-actin content in normal heart muscle of adult man and mouse. In end stage failing heart 53 +/- 5% of striated actin was skeletal muscle alpha-actin and in samples of inter-ventricular septum from patients with hypertrophic obstructive cardiomyopathy (HOCM) skeletal muscle alpha-actin was 72 +/- 2% of sarcomeric actin. Thin filaments containing actin isolated from normal and HOCM heart muscle were functionally indistinguishable when studied by quantitative in vitro motility assay. We also found elevated skeletal muscle alpha-actin (60 +/- 7%) in a mouse model of dilated cardiomyopathy.
Copeland
O'Neal
O
NHLI, Cardiovascular Science, Imperial College London, London, UK.
Nowak
Kristen J
KJ
Laing
Nigel G
NG
Ravenscroft
Gianina
G
Messer
Andrew E
AE
Bayliss
Christopher R
CR
Marston
Steven B
SB
D000199:Actins / Q000096:biosynthesis*; D000328:Adult; D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012518:Sarcomeres / Q000378:metabolism / Q000473:pathology
D000199:Actins