title: Immunopathology and cytokine responses in commercial broiler chickens with gangrenous dermatitis.
abstract: Gangrenous dermatitis (GD) is an emerging disease of increasing economic importance in poultry resulting from infection by Clostridium septicum and Clostridium perfringens type A. Lack of a reproducible disease model has been a major obstacle in understanding the immunopathology of GD. To gain better understanding of host-pathogen interactions in GD infection, we evaluated various immune parameters in two groups of birds from a recent commercial outbreak of GD, the first showing typical disease signs and pathological lesions (GD-like birds) and the second lacking clinical signs (GD-free birds). Our results revealed that GD-like birds showed: reduced T-cell and B-cell mitogen-stimulated lymphoproliferation; higher levels of serum nitric oxide and alpha-1-acid glycoprotein; greater numbers of K55(+), K1(+), CD8(+), and MHC class II(+) intradermal lymphocytes, and increased K55(+), K1(+), CD8(+), TCR1(+), TCR2(+), Bu1(+), and MHC class II(+) intestinal intraepithelial lymphocytes; and increased levels of mRNAs encoding proinflammatory cytokines and chemokines in skin compared with GD-free chickens. These results provide the first evidence of altered systemic and local (skin and intestine) immune responses in GD pathogenesis in chickens.
authors: Animal and Natural Resources Institute, USDA-ARS, Beltsville, MD, USA.
authors: Guangxing
authors: G
authors: Li
authors: Hyun S
authors: HS
authors: Lillehoj
authors: Kyung Woo
authors: KW
authors: Lee
authors: Sung Hyen
authors: SH
authors: Lee
authors: Myeong Seon
authors: MS
authors: Park
authors: Seung I
authors: SI
authors: Jang
authors: Gary R
authors: GR
authors: Bauchan
authors: Cyril G
authors: CG
authors: Gay
authors: G Donald
authors: GD
authors: Ritter
authors: Daniel A
authors: DA
authors: Bautista
authors: Gregory R
authors: GR
authors: Siragusa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D049109:Cell Proliferation; D018925:Chemokines / Q000276:immunology; D002645:Chickens; D003013:Clostridium / Q000235:genetics / Q000472:pathogenicity*; D016207:Cytokines / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D003691:Delaware; D003872:Dermatitis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005734:Gangrene / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000276:immunology*; D009569:Nitric Oxide / Q000097:blood; D009961:Orosomucoid / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D018925:Chemokines; D016207:Cytokines; D017931:DNA Primers; D009961:Orosomucoid; D009569:Nitric Oxide