[Effects of 2,2',4,4'-tetrabrominated diphenyl ethers on the TRalpha1 and TRbeta1 expression in liver in C57BL/6 mice].
OBJECTIVE
To study the effect of 2,2',4,4'-tetrabrominated diphenyl ethers (BDE-47) on the expression change of TRalpha1, TRbeta1 at both mRNA and protein levels.


METHODS
C57BL/6 mice were divided randomly into 4 groups and administrated with corn oil, 0.5, 5 and 50 mg/kg/BW BDE-47 for 4 days through intraperitoneal injection respectively. GAPDH selected as internal standard, real time quantitative RT-PCR and Western blot were employed to detect the mRNA levels and protein of TRalpha1 and TRbeta1 in livers.


RESULTS
Compared with the control group, the mRNA levels of TRalpha1 and TRbeta1 were up-regulated in the medium and high BDE-47 dose groups (P < 0.05). The protein level of TRalpha1 significantly up-regulated while TRbeta1 was significantly down-regulated.


CONCLUSIONS
BDE-47 could change the expression of TRalpha1 and TRbeta1 in the mRNA and protein levels in liver.
Liu
Zaoling
Z
School of Public Health of Xinjiang Medicial University, Urumchi 830054, China. 80858710173@sina.com
Liu
Jiwen
J
Zhang
Jianqing
J
Tian
Yanan
Y
Fang
Daokui
D
Huang
Haiyan
H
Jiang
Yousheng
Y
Zhou
Jian
J
Zhang
Hongyu
H
Mei
Shujiang
S
D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011075:Polybrominated Biphenyls; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics / Q000378:metabolism*; D037042:Thyroid Hormone Receptors beta / Q000235:genetics / Q000378:metabolism*
D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; D012333:RNA, Messenger; D037021:Thyroid Hormone Receptors alpha; D037042:Thyroid Hormone Receptors beta; C511295:2,2',4,4'-tetrabromodiphenyl ether