[Effects of retinoic acid receptor agonist and retinoid X receptor agonist on the expression of IL-4 and IFN-gamma in cord blood mononuclear cells].
OBJECTIVE
To observe the effects of retinoic acid receptors agonist TTNPB and retinoid X receptors agonist MA on both protein and gene expression of IFN-gamma and IL-4 in cultured cord blood mononuclear cells.


METHODS
Observing both the protein content and gene expression of IFN-gamma and IL-4 with ELISA and fluorescent real-time quantitative PCR in vitro.


RESULTS
It was indicated by ELISA that 10(-8), 10(-6), 10(-5) and 10(-4) mol/L TTNPB and 10(-8), 10(-5) and 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4 significantly in comparison with the control (P < 0.05), and indicated by RT-PCR that 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4mRNA significantly and 10(-5) mol/L TTNPB and 10(-7), 10(-6) mol/L MA increased the ratio of IFN-gamma/IL-4mRNA significantly in comparison with the control (P < 0.05).


CONCLUSIONS
The ratio of IFN-gamma/IL-4 could be down-regulated by proper concentration of TTNPB and MA at the protein level, while the ratio of IFN-gamma/IL-4mRNA was differently influenced by various concentration of TTNPB and MA at the gene level.
Zhu
Dongyang
D
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China. zdy213@126.com
Wang
Lili
L
Li
Yun
Y
Zhu
Yanfeng
Y
Zhao
Pinnan
P
D001565:Benzoates / Q000494:pharmacology; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000819:agonists* / Q000502:physiology; D047488:Retinoid X Receptors / Q000819:agonists* / Q000502:physiology; D012176:Retinoids / Q000494:pharmacology
D001565:Benzoates; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D012176:Retinoids; D015847:Interleukin-4; C038864:4-(2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic acid; D007371:Interferon-gamma