Mutations in the stalk region of the measles virus hemagglutinin inhibit syncytium formation but not virus entry.
Measles virus (MV) entry requires at least 2 viral proteins, the hemagglutinin (H) and fusion (F) proteins. We describe the rescue and characterization of a measles virus with a specific mutation in the stalk region of H (I98A) that is able to bind normally to cells but infects at a lower rate than the wild type due to a reduction in fusion triggering. The mutant H protein binds to F more avidly than the parent H protein does, and the corresponding virus is more sensitive to inhibition by fusion-inhibitory peptide. We show that after binding of MV to its receptor, H-F dissociation is required for productive infection.
Ennis
Matthew K
MK
Department of Molecular Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Hu
Chunling
C
Naik
Shruthi K
SK
Hallak
Louay K
LK
Peng
Kah Whye
KW
Russell
Stephen J
SJ
Dingli
David
D
D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015726:Giant Cells / Q000821:virology; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008459:Measles virus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020125:Mutation, Missense; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D053586:Virus Internalization
D006389:Hemagglutinins, Viral; D050505:Mutant Proteins; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus