Genotyping of fluconazole-resistant Candida albicans isolated from Uighurian people in Xinjing (China) using ALTS/RFLP and micro-TGGE method.
Antifungal susceptibility tests were performed for 38 Candida albicans strains isolated from oral cavities of 43 Uighurian AIDS patients. Results showed that six isolates were resistant to fluconazole; one showed low susceptibility. We attempted to examine these strains molecular-epidemiologically, but 25S rDNA genotyping was insufficient for their discrimination. To estimate whether the origins of resistant strains were identical, we developed a new combination method of C. albicans tandem repeating units (ALTS)/RFLP and micro-temperature gradient gel electrophoresis (micro-TGGE). This new method was able to distinguish all seven strains. A suspected nosocomial infection was ruled out.
Mijiti
Juhaer
J
Medical Mycology Research Center, Chiba University, Chiba, Japan.
Pu
Xiong Ming
XM
Erfan
Alshat
A
Yaguchi
Takashi
T
Chibana
Hiroji
H
Tanaka
Reiko
R
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D002681:China; D058645:Denaturing Gradient Gel Electrophoresis; D015725:Fluconazole / Q000494:pharmacology*; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences
D000935:Antifungal Agents; D015725:Fluconazole