Human plasma copper proteins speciation by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Solutions for columns calibration by sulfur detection.
Among the hyphenated techniques used to probe and identify metalloproteins, size exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) has shown to have a central place. However, the calibration of SEC columns reveals to be tedious and always involves UV detection prior to ICP-MS. The presence of sulfur in 98% of proteins allows their detection by quadrupole ICP-MS, despite the isobaric interference ((16)O(16)O) on S, by monitoring (32)S(16)O at mass to charge ratio (m/z) 48. The formation of SO occurs spontaneously in the argon plasma but can be optimized by the introduction of oxygen gas into a reaction cell (RC) to achieve nM levels. In this article, sulfur detection was discussed upon instrumental conditions and S detection was then optimized by applying O(2) as a reaction gas. SO formation was used to calibrate SEC columns without UV detection. This simple SEC-ICP-MS method was used for plasma copper proteins in plasma healthy subjects (HS) and an untreated Wilson disease (WD) patient. Copper proteins identified in healthy subjects were transcuprein, ceruloplasmin (Cp) and albumin. The method led to results in good agreement with other methods of determination. Copper bound to Cp in the WD patient was lowered with regard to the HS, and the exchangeable Cu was highly increased.
El Balkhi
Souleiman
S
Laboratoire de toxicologie biologique, AP-HP, Hôpital Lariboisière, 2, rue Ambroise Paré, 75475 Paris cedex 10, France.
Poupon
Joël
J
Trocello
Jean-Marc
JM
Massicot
France
F
Woimant
France
F
Laprévote
Olivier
O
D002138:Calibration; D002352:Carrier Proteins / Q000097:blood; D002570:Ceruloplasmin / Q000032:analysis; D002850:Chromatography, Gel / Q000379:methods* / Q000592:standards; D003300:Copper / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008667:Metalloproteins / Q000097:blood*; D010100:Oxygen / Q000737:chemistry; D012709:Serum Albumin / Q000032:analysis; D013455:Sulfur / Q000032:analysis*
D002352:Carrier Proteins; D008667:Metalloproteins; D012709:Serum Albumin; C023548:copper-binding protein; D013455:Sulfur; D003300:Copper; D002570:Ceruloplasmin; D010100:Oxygen