[Effects of 9,10-dihydroxysteatic acid on glucose metabolism in KKAy mice].
OBJECTIVE
To study the effect of 9,10-dihydroxystearic acid (DHSA) on glucose tolerance and insulin sensitivity in KKAy diabetic mice.


METHODS
48 male KKAy mice were randomly divided into four groups and were fed with high fat diet containing 4% DHSA, 2% DHSA, 4% Olive oil and 4% Corn oil, respectively. Glucose tolerance and insulin sensitivity were measured after 5 and 6 weeks respectively. Effects of DHSA on activating PPAR-alpha and PPAR-gamma in CV-1 cells were also carried out.


RESULTS
In glucose tolerance test, the blood glucose at 0.5 h and 1 h and the area under glucose curve in the group fed with 4% DHSA diet were significantly lower than those of other groups. In insulin sensitivity test, the blood glucose level at 0.5 h and 1 h after injection of insulin was lower in the 4% DHSA diet group, indicating the insulin sensitivity of this group was obviously higher than other groups (P < 0.05). The body weight of the mice fed with 4% DHSA diet was much lower than those mice fed with 4% Corn diet (P < 0.05). 5 - 20 micromol/L DHSA did not activate PPAR-gamma in CV-1 cells but 50 - 100 micromol/L DHSA activated PPARgamma in a dose-dependent way although the activating effect of DHSA on PPARgamma was significantly lower than that of rosiglitazone. DHSA did not activate PPAR-alpha in CV-1 cells.


CONCLUSIONS
DHSA may improve glucose tolerance and insulin sensitivity in KKAy mice and the effect might be related to the activation of PPAR-gamma by DHSA.
Yu
Xiaoming
X
Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
Zhang
Yuehong
Y
Liu
Yinghua
Y
Wang
Jin
J
Xu
Qing
Q
Zhang
Xinsheng
X
Jing
Hongjiang
H
Zhang
Yong
Y
Zhang
Rongxin
R
Xue
Changyong
C
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D059305:Diet, High-Fat; D005951:Glucose Tolerance Test; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D047495:PPAR gamma / Q000378:metabolism; D011897:Random Allocation; D013229:Stearic Acids / Q000494:pharmacology*
D001786:Blood Glucose; D047495:PPAR gamma; D013229:Stearic Acids; C074110:9,10-dihydroxystearic acid