title: Increased subventricular zone-derived cortical neurogenesis after ischemic lesion.
abstract: In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in peri-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone.
authors: Department of Clinical Neurobiology, Interdisciplinary Center for Neurosciences, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
authors: Maria
authors: M
authors: Kreuzberg
authors: Evgeny
authors: E
authors: Kanov
authors: Oleg
authors: O
authors: Timofeev
authors: Markus
authors: M
authors: Schwaninger
authors: Hannah
authors: H
authors: Monyer
authors: Konstantin
authors: K
authors: Khodosevich
mesh_terms: D000818:Animals; D000963:Antimetabolites; D017209:Apoptosis / Q000502:physiology; D002545:Brain Ischemia / Q000473:pathology*; D001973:Bromodeoxyuridine; D064032:Calbindin 2; D053148:Caspase 3 / Q000096:biosynthesis; D002540:Cerebral Cortex / Q000166:cytology / Q000473:pathology*; D002552:Cerebral Ventricles / Q000166:cytology / Q000473:pathology*; D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D007395:Interneurons / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D017397:Prefrontal Cortex / Q000473:pathology*; D044406:Receptors, Serotonin, 5-HT3 / Q000096:biosynthesis / Q000235:genetics; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D000963:Antimetabolites; D064032:Calbindin 2; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D044406:Receptors, Serotonin, 5-HT3; D064030:S100 Calcium Binding Protein G; D049452:Green Fluorescent Proteins; D005680:gamma-Aminobutyric Acid; D053148:Caspase 3; D001973:Bromodeoxyuridine