title: Evaluation of oxidative DNA lesions in plasma and nuclear abnormalities in erythrocytes of wild fish (Liza aurata) as an integrated approach to genotoxicity assessment.
abstract: Genetic lesions (8-hydroxy-2'-deoxyguanosine (8-OHdG) and erythrocytic nuclear abnormalities (ENA) were seasonally quantified in the blood of Liza aurata caught at Ria de Aveiro (Portugal), a multi-contaminated aquatic system. Thus, five critical sites were assessed and compared with a reference site (Torreira). Oxidative DNA damage was found in Gafanha (harbour-water area), Laranjo (metal-contaminated) and Vagos (contaminated with PAHs) in the spring; Rio Novo do Principe (near a former paper-mill effluent) in the autumn; Rio Novo do Principe and Vagos in the winter. ENA were higher than Torreira at VAG (spring and winter). Torreira did not display seasonal variation neither in terms of 8-OHdG or total ENA. A positive correlation between 8-OHdG and ENA was found, suggesting oxidative stress as a mechanism involved in the formation of ENA. This study clearly demonstrates the presence of DNA-damaging substances in Ria de Aveiro and recommends the use of 8-OHdG and ENA as biomarkers of environmental contamination.
authors: CESAM & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal. migueloliveira@ua.pt
authors: M
authors: M
authors: Oliveira
authors: I
authors: I
authors: Ahmad
authors: V L
authors: VL
authors: Maria
authors: C S S
authors: CS
authors: Ferreira
authors: A
authors: A
authors: Serafim
authors: M J
authors: MJ
authors: Bebianno
authors: M
authors: M
authors: Pacheco
authors: M A
authors: MA
authors: Santos
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D053144:Cell Nucleus Shape; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004784:Environmental Monitoring; D004912:Erythrocytes / Q000648:ultrastructure*; D048629:Micronuclei, Chromosome-Defective; D009152:Mutagenicity Tests / Q000379:methods*; D018384:Oxidative Stress; D011174:Portugal; D023701:Smegmamorpha / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine