Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond.
An alkalitolerant and moderately halophilic strain, designated KB23(T), characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7 % (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23(T) were Gram-stain-positive, motile straight rods. Strain KB23(T) was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C(15 : 0), C(16 : 0) and anteiso-C(15 : 1) were the major cellular fatty acids. The DNA G+C content of strain KB23(T) was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109(T) (97.35 % similarity), Cellulomonas terrae DB5(T) (96.81 %), Cellulomonas iranensis O(T) (96.75), Cellulomonas chitinilytica X.bu-b(T) (96.60 %), Cellulomonas persica I(T) (96.53 %), Cellulomonas composti TR7-06(T) (96.45 %), Cellulomonas biazotea DSM 20112(T) (96.34 %) and Cellulomonas fimi DSM 20113(T) (96.20 %). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23(T) is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23(T) ( = DSM 22512(T)  = NCAIM B002303(T)).
Rusznyák
Anna
A
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Tóth
Erika M
EM
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Schumann
Peter
P
DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Spröer
Cathrin
C
DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Makk
Judit
J
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Szabó
Gitta
G
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Vladár
Péter
P
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Márialigeti
Károly
K
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
Borsodi
Andrea K
AK
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D040143:Cellulomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006814:Hungary; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D006109:Poaceae / Q000382:microbiology*; D061005:Ponds; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9