title: Differential epithelial cell response upon stimulation with the Aggregatibacter actinomycetemcomitans strains VT 1169, VT 1560 DAM⁻ and ATCC 4318.
abstract: Aggregatibacter actinomycetemcomitans is known to play a role in human inflammatory oral diseases. The present study aimed to test whether GECs from different individuals show an altered immune response when exposed to wild-type A. actinomycetemcomitans biofilms (VT 1169 and ATCC43718) versus VT 1560 lacking a DNA-adenine-methyltransferase (DAM⁻). GECs were cultured from biopsies derived from three healthy subjects (A, B, and C). To obtain A. actinomycetemcomitans biofilms, each strain (VT 1169, ATCC 43718, and VT 1560 DAM⁻) was separately cultured on polymer disks. The mRNA expression of hBD-2, Rnase7, IL-8, and glycerylaldehyd-3-phosphodehydrogenase was analyzed using semi-quantitative RT-PCR. In GECs, the DAM⁻ strain VT 1560 led to a reduced gene expression of hBD-2 and IL-8 compared to VT 1169, but not ATCC 43718. In GECs from subject C, the mRNA expression of hBD-2 and IL-8 was significantly up regulated in response to A. actinomycetemcomitans VT 1169 compared to VT 1560 DAM⁻ and ATCC 43718 (p≤0,05), whereas GECs from subject A and B did not show significant changes for hBD-2 or IL-8 gene expression upon stimulation with either of the strains tested. The present study indicates strain-dependent and subject-specific immune responses in GECs after exposure to DAM(+) or DAM⁻ A. actinomycetemcomitans.
authors: Department of Prostethic Dentistry, Hannover Medical School, Germany. eberhard.joerg@mh-hannover.de
authors: Jörg
authors: J
authors: Eberhard
authors: Tobias
authors: T
authors: Banasch
authors: Søren
authors: S
authors: Jepsen
authors: Henrik
authors: H
authors: Dommisch
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D009059:Mouth Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D015661:Pasteurellaceae / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000276:immunology; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
chemical_list: D001426:Bacterial Proteins; D012333:RNA, Messenger; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)