title: Preconditioning with Maillard reaction products improves antioxidant defence leading to increased stress tolerance in cardiac cells.
abstract: Advanced glycation end products (AGEs) are considered as biomarkers of ageing and are associated with several degenerative diseases. Besides endogenous formation, significant amounts of AGEs are taken up with food. Although nutritional AGEs are considered as undesirable, proinflammatory agents, they may also enclose potentially beneficial antioxidants. We used rodent cardiac cells to evaluate if food AGEs, present in bread crust, can modify the cellular antioxidant defence. Mice were fed with bread crust containing diet to prove the in-vivo relevance for the heart. In mouse cardiac fibroblasts, bread crust extract induced a moderate elevation of ROS production causing an activation of p42/p44(MAPK), p38(MAPK) and NF-κB, followed by increased expression of antioxidative enzymes. Preconditioning studies demonstrated that this was sufficient to protect cardiac fibroblasts and rat adult cardiac myocytes against severe oxidative stress. Furthermore, mice, fed a bread crust containing diet, exhibited a similarly improved cardiac expression of antioxidative defence genes. The consumption of AGEs can therefore contribute to an improved antioxidant status of the heart, thus exhibiting cardioprotective effects in case of severe oxidative stress as in ischemia reperfusion injury. Also, these data show that the exclusive interpretation of circulating AGEs as pathophysiological biomarkers of ageing might be misleading.
authors: Department of Cardiothoracic Surgery, Martin-Luther University Halle-Wittenberg, Germany.
authors: Stefanie
authors: S
authors: Ruhs
authors: Norbert
authors: N
authors: Nass
authors: Babett
authors: B
authors: Bartling
authors: Hans-Jürgen
authors: HJ
authors: Brömme
authors: Beatrice
authors: B
authors: Leuner
authors: Veronika
authors: V
authors: Somoza
authors: Ulrich
authors: U
authors: Friess
authors: Rolf-Edgar
authors: RE
authors: Silber
authors: Andreas
authors: A
authors: Simm
mesh_terms: D000375:Aging / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001939:Bread; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000097:blood / Q000494:pharmacology; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology; D015416:Maillard Reaction; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced