Neurogenic characteristics of placental stem cells in preeclampsia.
OBJECTIVE
Preeclampsia is associated with perinatal brain injury. Autologous placenta stem cell transplantation represents a promising future treatment option for neuroregeneration. The aim of this study was to compare the neuroregenerative capacity of preeclampsia-placenta stem cells to previously characterized placentas from uncomplicated pregnancies.


METHODS
Placenta stem cells from amnion (epithelium, mesenchyme) and chorion were assessed for cell surface markers and the formation of neuronal-like cells, oligodendrocytes and their progenitors in culture.


RESULTS
Markers of preeclampsia-placenta stem cells were different from uncomplicated pregnancies-placenta stem cells in amnion epithelium and chorion, but not in amnion mesenchyme. Similarly to uncomplicated pregnancies-placenta stem cells, preeclampsia-placenta stem cells derived from amnion and chorion differentiated preferably into nestin-positive stem/progenitor cells and Tuj-1-positive neurons. However, other important markers were varying after neurogenic differentiation of uncomplicated pregnancies- and preeclampsia-placenta stem cells.


CONCLUSIONS
Surface marker expression patterns of preeclampsia-placenta stem cell's and uncomplicated pregnancies-placenta stem cell's differ. In vitro differentiation assays, however, provide evidence that both preeclampsia-placenta stem cells and uncomplicated pregnancies-placenta stem cells are comparably suitable for neuroregeneration purposes.
Portmann-Lanz
C Bettina
CB
Department of Obstetrics and Gynecology, University Hospital, Berne, Switzerland.
Baumann
Marc U
MU
Mueller
Martin
M
Wagner
Anna-Margaretha
AM
Weiss
Salome
S
Haller
Odett
O
Sager
Ruth
R
Reinhart
Ursula
U
Surbek
Daniel V
DV
D000650:Amnion / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002823:Chorion / Q000166:cytology; D005260:Female; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010920:Placenta / Q000166:cytology*; D011225:Pre-Eclampsia / Q000473:pathology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*; D014404:Tubulin / Q000378:metabolism
D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; C498585:TUBB3 protein, human; D014404:Tubulin