Separation and determination of four ganoderic acids from dried fermentation mycelia powder of Ganoderma lucidum by capillary zone electrophoresis.
Ganoderic acids (GAs) were bioactive secondary metabolites produced by a traditional mushroom Ganoderma lucidum. We describe a simple and efficient method for the separation and quantitative determination of four GAs, namely Ganoderic acid T (GA-T), Ganoderic acid Mk (GA-Mk), Ganoderic acid Me (GA-Me) and Ganoderic acid S (GA-S) from dried triterpene-enriched extracts of G. lucidum mycelia powder by capillary zone electrophoresis (CZE). Under the optimum conditions, the four GAs reached the baseline separation in 9 min with Glycyrrhetinic acid (GTA) as internal standard. The four GAs and internal standard (GTA) were detected at a wavelength 245 nm. All calibration curves showed good linearity (r(2)>0.9958) within test ranges. Limit of detection (LOD) and limit of quantification (LOQ) were less than 0.6 and 1.8 microg/mL, respectively. The relative standard deviation (R.S.D.) values of precision and recoveries were less than 5% and recoveries ranged from 91.4% to 103.6%. This was the first report on simultaneous determination of the four GAs and the results provided a firm basis for the trace analysis of GAs in dried fermentation mycelia powder of G. lucidum with high accuracy.
Ding
Na
N
Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Yang
Qing
Q
Huang
Sha-Sheng
SS
Fan
Liu-Yin
LY
Zhang
Wei
W
Zhong
Jian-Jiang
JJ
Cao
Cheng-Xi
CX
D000970:Antineoplastic Agents / Q000032:analysis / Q000592:standards; D002138:Calibration / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000592:standards; D019075:Electrophoresis, Capillary / Q000379:methods; D005285:Fermentation; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000032:analysis / Q000592:standards; D025282:Mycelium; D011208:Powders; D020940:Reishi / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis* / Q000592:standards
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D011208:Powders; D014315:Triterpenes; C534033:ganoderic acid Me; C550530:ganoderic acid T; D007810:Lanosterol