Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment.
A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.
Kim
Byung-Hyuk
BH
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-333, Republic of Korea.
Baek
Kyung-Hwa
KH
Cho
Dae-Hyun
DH
Sung
Youlboong
Y
Koh
Sung-Cheol
SC
Ahn
Chi-Yong
CY
Oh
Hee-Mock
HM
Kim
Hee-Sik
HS
D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002713:Chlorine / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005030:Ethylenes / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D019344:Lactic Acid / Q000378:metabolism; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013750:Tetrachloroethylene / Q000378:metabolism*; D013997:Time Factors
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005030:Ethylenes; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D002713:Chlorine; C036216:ethylene; D013750:Tetrachloroethylene