title: Fluorouracil selectively enriches stem-like leukemic cells in a leukemic cell line.
abstract: Recent studies have reported that cancer stem cells (CSCs) could be isolated from solid cancer cell lines, in which the purity of CSCs was higher than that from tumor tissues. Separation of CSCs from leukemic cell lines was rarely reported. In this study, CD34(+)CD38(-)stem-like cell subsets in human KG-1a leukemic cell line were enriched by cytotoxic agent 5-fluorouracil (5-FU). After 4 days incubation of KG-1a cell line with 5-FU (50 microg/ml), the CD34(+)CD38(-) subpopulation of cell lines was enriched more than 10 times. The enriched cells had proliferate potential in vitro, low level of RNA transcription and Hoechst 33342 dye efflux ability, accompanied by high expression of ATP-binding cassette transporter protein ABCG2. Our findings suggest that treatment with 5-FU offers an easy method to isolate leukemic stem-like subpopulation. It can facilitate studies of leukemic stem cell biology and the development of new therapeutic strategies.
authors: 1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Key Laboratory, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. cqumszhl@sina.com
authors: Ling
authors: L
authors: Zhang
authors: Song
authors: S
authors: Yang
authors: Yu-Juan
authors: YJ
authors: He
authors: Hui-Yuan
authors: HY
authors: Shao
authors: Li
authors: L
authors: Wang
authors: Hui
authors: H
authors: Chen
authors: Yu-Jie
authors: YJ
authors: Gao
authors: Feng-Xian
authors: FX
authors: Qing
authors: Xian-Chun
authors: XC
authors: Chen
authors: Liu-Yang
authors: LY
authors: Zhao
authors: Shi
authors: S
authors: Tan
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001562:Benzimidazoles / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D012313:RNA / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D018952:Antigens, CD34; D001562:Benzimidazoles; D009363:Neoplasm Proteins; D012313:RNA; D004247:DNA; D051997:ADP-ribosyl Cyclase 1; C017807:bisbenzimide ethoxide trihydrochloride; D005472:Fluorouracil