Antioxidant, larvicidal and antiacetylcholinesterase activities of cashew nut shell liquid constituents.
Anacardic acid, cardanol and cardol, the main constituents of natural cashew nut shell liquid (CNSL), were obtained by solvent extraction and assayed for antioxidant, larvicidal and antiacetylcholinesterase activity. Their relative percent composition was obtained by HPLC analysis. Antioxidant activity was assessed using the DPPH and ABTS(+) tests, which showed cardanol as the most active, followed by cardol and anacardic acid. The three CNSL components demonstrated good larvicidal activity against Aedes aegypti (LC(50)=12.40 for anacardic acid, 10.22 for cardol and 14.45 for cardanol) and exhibited inhibition zones for acetylcholinesterase enzymes in the TLC test similar to carbachol, which was used as standard. Based on the results, these multipotent compounds represent promising agents in the control of Ae. aegypti, the main dengue vector in Brazil.
Oliveira
Micheline Soares Costa
MS
Doutorado de Biotecnologia da Rede Nordeste de Biotecnologia, Av. Paranjana 1700, Campus do Itaperi, Serrinha, Fortaleza, Ceará, Brazil.
Morais
Selene Maia de
SM
Magalhães
Davi Varela
DV
Batista
Williams Pereira
WP
Vieira
Icaro Gusmão Pinto
IG
Craveiro
Afrânio Aragão
AA
de Manezes
Jane Eire Silva Alencar
JE
Carvalho
Ana Fontenelle Urano
AF
de Lima
Glauber Pacelli Gomes
GP
D000110:Acetylcholinesterase / Q000032:analysis / Q000378:metabolism; D000330:Aedes / Q000187:drug effects / Q000254:growth & development; D048729:Anacardic Acids / Q000494:pharmacology; D031021:Anacardium / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D003715:Dengue / Q000517:prevention & control; D003716:Dengue Virus; D004199:Disease Vectors; D020128:Inhibitory Concentration 50; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D009032:Mosquito Control / Q000379:methods; D009754:Nuts / Q000737:chemistry* / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology; D012118:Resorcinols / Q000494:pharmacology
D048729:Anacardic Acids; D000975:Antioxidants; D010636:Phenols; D012118:Resorcinols; C088115:anacardic acid; C038590:cardanol; D000110:Acetylcholinesterase; C041328:adipostatin A