Acute toxicity of toluene determined using glioma cells contained in sealed rolling bottles, with controlled vapour concentration.
A rolling bottle system containing glioma (C6) cells was used to test the toxicity of toluene, using mitochondrial activity (MTT assay), neutral red uptake and cell growth as indicators of toxicity. Toluene was shown to have toxic effects only at gas concentrations above 12,000 ppm. Because tests showed that as much as 30% of toluene leaked out of sealed tubes filled with toluene gas, the concentrations of toluene gas in the tubes were monitored by gas chromatography.
Ryghseter
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University of Trondheim, Faculty of Medicine, Department of Pharmacology and Toxicology, N-7000 Trondheim, Norway.
Jenssen
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Syversen
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