title: Methylation patterns of Brahma during spermatogenesis and oogenesis: potential implications.
abstract: To compare methylation profiles and expression levels of Brahma at different stages of spermatogenesis, and to identify the methylation pattern during oogenesis, we analyzed gene expression and methylation patterns in murine germ cells at various developmental stages. The methylation levels of CpG islands within Brahma increased during spermatogenesis and decreased during oogenesis. This change in methylation pattern correlates with the change in expression of Brahma during spermatogenesis. As the degree of methylation increases, the expression decreases. The change in methylation is opposite during oogenesis, which suggests opposite expression levels.
authors: Laboratory of Clinical and Developmental Genomics, Program in Reproductive and Adult Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20814, USA. nagranisr@mail.nih.gov
authors: Sohan R
authors: SR
authors: Nagrani
authors: Eric D
authors: ED
authors: Levens
authors: Vanessa
authors: V
authors: Baxendale
authors: Catherine
authors: C
authors: Boucheron
authors: Wai Yee
authors: WY
authors: Chan
authors: Owen M
authors: OM
authors: Rennert
mesh_terms: D002843:Chromatin / Q000502:physiology; D018899:CpG Islands / Q000502:physiology; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D006801:Humans; D007985:Leydig Cells / Q000502:physiology; D008297:Male; D009865:Oocytes / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D012708:Sertoli Cells / Q000502:physiology; D013090:Spermatocytes / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D002843:Chromatin; C084011:SMARCA2 protein, human; D014157:Transcription Factors