Central administration of kisspeptin-10 inhibits water and sodium excretion of anesthetized male rats and the involvement of arginine vasopressin.
OBJECTIVE
To investigate the effect of hypothalamus kisspeptin on water and sodium excretion and the possible mechanism.


METHODS
The intracerebroventricular (icv) administration and radioimmunoassay were used to observe the effect of kisspeptin-10 on urine flow, sodium and potassium excretion, plasma arginine vasopressin (AVP), and atrial natriuretic peptide (ANP) concentrations in anesthetized male rats. The mediation of renal sympathetic nerve was also investigated by studies conducted on rats with bilateral renal sympathetic denervation.


RESULTS
The urine flow, sodium excretion, and free water clearance decreased significantly by icv injection of 5 nmol kisspeptin-10 (p < 0.05) from 30 to 60 min post-injection. Meanwhile, plasma AVP concentrations increased significantly 30 min after the icv injection of 5 nmol kisspeptin-10 (p < 0.05), whereas the equal dose of kisspeptin-10 did not significantly change plasma ANP concentrations. The mean arterial blood pressure, heart rate, and potassium excretion did not significantly change during the experiment. Furthermore, pretreatment with 5 nmol kisspeptin-10 could still significantly decrease urine flow and sodium excretion in renal sympathetic denervated rats.


CONCLUSIONS
Central administration of kisspeptin-10 could inhibit sodium excretion and urine flow in anesthetized male rats, which is probably mediated by increasing the plasma AVP concentration and is independent of plasma ANP concentration and renal sympathetic nerve activity.
Ten
Shi-Chao
SC
Department of Endocrinology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, PR China.
Gu
Shou-Yong
SY
Niu
Yun-Fei
YF
An
Xiao-Fei
XF
Yan
Ming
M
He
Ming
M
D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood*; D009320:Atrial Natriuretic Factor / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects; D004231:Diuresis / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D007276:Injections, Intraventricular; D007668:Kidney / Q000294:innervation; D059648:Kisspeptins; D008297:Male; D009318:Natriuresis / Q000187:drug effects*; D009842:Oligopeptides / Q000008:administration & dosage*; D011188:Potassium / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013562:Sympathectomy; D013564:Sympathetic Nervous System / Q000502:physiology
C106628:KISS1 protein, human; D059648:Kisspeptins; D009842:Oligopeptides; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor; D011188:Potassium