title: Modelling acute renal failure using blood and breath biomarkers in rats.
abstract: This paper compares three methods for estimating renal function, as tested in rats. Acute renal failure (ARF) was induced via a 60-min bilateral renal artery clamp in 8 Sprague-Dawley rats and renal function was monitored for 1 week post-surgery. A two-compartment model was developed for estimating glomerular filtration via a bolus injection of a radio-labelled inulin tracer, and was compared with an estimated creatinine clearance method, modified using the Cockcroft-Gault equation for rats. These two methods were compared with selected ion flow tube-mass spectrometry (SIFT-MS) monitoring of breath analytes. Determination of renal function via SIFT-MS is desirable since results are available non-invasively and in real time. Relative decreases in renal function show very good correlation between all 3 methods (R²=0.84, 0.91 and 0.72 for breath-inulin, inulin-creatinine, and breath-creatinine correlations, respectively), and indicate good promise for fast, non-invasive determination of renal function via breath testing.
authors: Department of Mechanical Engineering, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand. ktm19@student.canterbury.ac.nz
authors: Katherine T
authors: KT
authors: Moorhead
authors: Jonathan V
authors: JV
authors: Hill
authors: J Geoffrey
authors: JG
authors: Chase
authors: Christopher E
authors: CE
authors: Hann
authors: Jennifer M
authors: JM
authors: Scotter
authors: Malina K
authors: MK
authors: Storer
authors: Zoltan H
authors: ZH
authors: Endre
mesh_terms: D058186:Acute Kidney Injury / Q000503:physiopathology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D005919:Glomerular Filtration Rate; D013058:Mass Spectrometry; D008962:Models, Theoretical; D051381:Rats
chemical_list: D015415:Biomarkers