title: A novel disulfide pattern in laminin-type epidermal growth factor-like (LE) modules of laminin β1 and γ1 chains.
abstract: In-depth mass spectrometric analysis of disulfide bond patterns in recombinant mouse laminin β1 and γ1 chain N-terminal fragments comprising the laminin N-terminal (LN) domain and the first four laminin epidermal growth factor-like (LE) domains revealed a novel disulfide pattern for LE domains. This showed a (2-3, 4-5, 6-7, 8-1) connectivity with the last cysteine of one LE domain being connected to the first cysteine of the following LE domain. The same pattern was also found in E4, the N-terminal β1 chain fragment derived by elastase digestion of mouse EHS tumor laminin-111, showing that this pattern occurs in native laminin. The strictly linear pattern with an interdomain disulfide has not been described previously for EGF domains. The N-terminal portions of laminin short arms, consisting of the LN domain and LE domains 1-4, are essential for laminin-laminin self-interactions, whereas the internal LE domains 7-9 in the laminin γ1 chain harbor the nidogen binding site and have a conventional disulfide pattern. This suggests that LE domains differing in function also differ in their disulfide patterns.
authors: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
authors: Stefan
authors: S
authors: Kalkhof
authors: Konstanze
authors: K
authors: Witte
authors: Christian H
authors: CH
authors: Ihling
authors: Mathias Q
authors: MQ
authors: Müller
authors: Manuel V
authors: MV
authors: Keller
authors: Sebastian
authors: S
authors: Haehn
authors: Neil
authors: N
authors: Smyth
authors: Mats
authors: M
authors: Paulsson
authors: Andrea
authors: A
authors: Sinz
mesh_terms: D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D004220:Disulfides; C513277:Lamb1 protein, mouse; D007797:Laminin; C089141:laminin gamma 1; D004815:Epidermal Growth Factor; D003545:Cysteine