Transcriptional regulation of a brown adipocyte-specific gene, UCP1, by KLF11 and KLF15.
Several growth factors and transcription factors have been reported to play important roles in brown adipocyte differentiation and modulation of thermogenic gene expression, especially the expression of UCP1. In this study, we focused on KLF11 and KLF15, which were expressed highly in brown adipose tissue. Our data demonstrated that KLF11 and KLF15 interacted directly with the UCP1 promoter using GC-box and GT-boxes, respectively. Co-transfection of KLF11 and KLF15 in the mesenchymal stem cell line muBM3.1 during brown adipocyte differentiation enhanced the expression level of UCP1. KLF11, but not KLF15, was essential for UCP1 expression during brown adipocyte differentiation of muBM3.1.
Yamamoto
Ken-ichi
K
Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Sakaguchi
Masakiyo
M
Medina
Reinhold J
RJ
Niida
Aya
A
Sakaguchi
Yoshihiko
Y
Miyazaki
Masahiro
M
Kataoka
Ken
K
Huh
Nam-ho
NH
D052437:Adipocytes, Brown / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D059630:Mesenchymal Stem Cells; D024101:Mitochondrial Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic; D000071256:Uncoupling Protein 1
D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; D007473:Ion Channels; C115117:KLF11 protein, human; C414172:KLF15 protein, human; D051741:Kruppel-Like Transcription Factors; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1