Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens.
A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.
White
P Lewis
PL
Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom. lewis.white@wales.nhs.uk
Perry
Michael D
MD
Loeffler
Juergen
J
Melchers
Willem
W
Klingspor
Lena
L
Bretagne
Stephane
S
McCulloch
Elaine
E
Cuenca-Estrella
Manuel
M
Finnstrom
Niklas
N
Donnelly
J Peter
JP
Barnes
Rosemary A
RA
D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics*; D001769:Blood / Q000382:microbiology*; D004271:DNA, Fungal / Q000302:isolation & purification*; D006801:Humans; D009172:Mycology / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity
D004271:DNA, Fungal