Cloning and comparative studies of seaweed trehalose-6-phosphate synthase genes.
The full-length cDNA sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of Porphyra yezoensis (PyTPS) was isolated by RACE-PCR and deposited in GenBank (NCBI) with the accession number AY729671. PyTPS encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 Daltons. The PyTPS protein consists of a TPS domain in the N-terminus and a putative TPP domain at the C-terminus. Homology alignment for PyTPS and the TPS proteins from bacteria, yeast and higher plants indicated that the most closely related sequences to PyTPS were those from higher plants (OsTPS and AtTPS5), whereas the most distant sequence to PyTPS was from bacteria (EcOtsAB). Based on the identified sequence of the PyTPS gene, PCR primers were designed and used to amplify the TPS genes from nine other seaweed species. Sequences of the nine obtained TPS genes were deposited in GenBank (NCBI). All 10 TPS genes encoded peptides of 908 amino acids and the sequences were highly conserved both in nucleotide composition (>94%) and in amino acid composition (>96%). Unlike the TPS genes from some other plants, there was no intron in any of the 10 isolated seaweed TPS genes.
Wang
Guoliang
G
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. mgbl_01@ouc.edu.cn
Zhao
Ge
G
Feng
Yanbin
Y
Xuan
Jinsong
J
Sun
Jianwei
J
Guo
Baotai
B
Jiang
Guoyong
G
Weng
Manli
M
Yao
Jianting
J
Wang
Bin
B
Duan
Delin
D
Liu
Tao
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D044664:Porphyra / Q000235:genetics*; D016415:Sequence Alignment
D017931:DNA Primers; D018076:DNA, Complementary; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase