Microbial adhesion to surface-grafted polyacrylamide brushes after long-term exposure to PBS and reconstituted freeze-dried saliva.
Polyacrylamide (PAAm) brushes, covalently grafted from silicon wafer surfaces were examined for their ability to inhibit microbial adhesion after long-term exposure to PBS or reconstituted freeze-dried saliva for time intervals from 48 h up to 1 month at 37 degrees C. Microbial adhesion after exposure was studied in a parallel plate flow chamber. Infrared spectra showed that PAAm brushes exhibit good chemical stability upon incubation in both PBS and reconstituted freeze-dried saliva up to 1 month. Reductions in microbial adhesion on PAAm brushes after exposure to PBS or reconstituted freeze-dried saliva varied from 63 to 93% depending on the microbial strain considered, even after 1 month of exposure of the brushes to reconstituted freeze-dried saliva.
Fundeanu
Irina
I
Department of Polymer Chemistry, Zernike Institute of Advanced Materials, University of Groningen, Groningen, The Netherlands.
van der Mei
Henny C
HC
Schouten
Arend J
AJ
Busscher
Henk J
HJ
D000180:Acrylic Resins / Q000737:chemistry*; D001422:Bacterial Adhesion / Q000502:physiology*; D002021:Buffers; D005612:Freeze Drying; D010710:Phosphates / Q000494:pharmacology; D012463:Saliva / Q000737:chemistry*; D013499:Surface Properties; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002021:Buffers; D010710:Phosphates; D000099166:Polyacrylamides