Scalable synthetic oligodeoxynucleotide purification with use of a catching by polymerization, washing, and releasing approach.
Synthetic oligodeoxynucleotides are purified with use of a catching by polymerization, washing, and releasing approach. The method does not require any chromatography, and purification is achieved by simple operations such as shaking, washing, and extraction. It is therefore useful for large-scale purification of synthetic oligonucleotide drugs. In addition to purification of oligonucleotides, this catching by polymerization concept is expected to be equally useful for purification of other synthetic oligomers such as peptides and oligosaccharides.
Fang
Shiyue
S
Department of Chemistry, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931, USA. shifang@mtu.edu
Fueangfung
Suntara
S
D002845:Chromatography / Q000379:methods; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D011108:Polymers