Neuroprotective effects of stanniocalcin 2 following kainic acid-induced hippocampal degeneration in ICR mice.
Stanniocalcin 2 (STC2), the paralog of STC1, has been shown to act as a novel target of the mammalian unfolded protein response. We investigated the potential neuroprotective actions of STC2 against kainic acid toxicity in the hippocampus of ICR mice. STC2-treated mice experienced less neuronal cell loss in the CA3 area of the hippocampus. Also, microglial activation and heme oxygenase 1 expression were attenuated in the hippocampus of STC2-treated mice. To confirm whether STC2 regulates microglial activation directly, nitric oxide levels were measured in BV2 cells cultured with and without 10nM STC2. STC2 decreased the level of nitric oxide induced by lipopolysaccharide (LPS) treatment significantly. Also, STC2 pretreatment significantly decreased TNF-α and IL-1β expression induced by LPS treatment. These observations demonstrated that STC2 exerts neuroprotective actions against excitotoxic insults through the inhibition of microglial activation.
Byun
Jong-Seon
JS
Department of Pharmacology, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea.
Lee
Jae-Won
JW
Kim
Su Young
SY
Kwon
Kwang Jun
KJ
Sohn
Jong-Hee
JH
Lee
Kyunyoung
K
Oh
Dahlkyun
D
Kim
Sung-Soo
SS
Chun
Wanjoo
W
Lee
Hee Jae
HJ
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006624:Hippocampus / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D053583:Interleukin-1beta / Q000096:biosynthesis; D047908:Intracellular Signaling Peptides and Proteins; D007608:Kainic Acid / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D053583:Interleukin-1beta; D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; C485438:Stc2 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051547:Heme Oxygenase-1; D007608:Kainic Acid