[Immunogenic and protective characteristics of surface antigens 6 and d of Burkholderia pseudomallei].
OBJECTIVE
Extraction of complex of Burkholderia pseudomallei antigens 6+d (Ag6+d) and study of its immunogenic and protective characteristics.


METHODS
Studied antigens were obtained from acrtone-dried cells of B. pseudomallei 57576. Experiments were performed on white mouse model. Microbiological, immunochemical as well as immunological methods were used in the study.


RESULTS
It was shown that antigenic complex 6+d has a glycoprotein nature and corresponds to high-polymeric catode-moving antigen 6 with molecular mass 500 kDa and anode-moving antigen d with molecular mass 40 - 55 kDa. Immunogenic and protective characteristics of surface antigenic complex 6+d was studied. It was noted that Ag6+d caused reliable stimulating effect on cellular arm of immune system of white mice appeared in activation of delayed-type hypersensitivity reactions, enhanced phagocytic activity of polymorphonuclear macrophages as well as increased expression of Fc-receptors on macrophages and neutrophils.


CONCLUSIONS
Observed stimulation of cellular immunity by surface antigens was confirmed during study of their protective characteristics on the model of experimental meloidosis in white mice.
Avrorova
I V
IV
Korsakova
I I
II
Zhukova
S I
SI
Khrapova
N P
NP
Dem'ianova
O B
OB
Lomova
L V
LV
Napalkova
G M
GM
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology; D006968:Hypersensitivity, Delayed; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D008554:Melioidosis / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism; D010587:Phagocytosis; D011961:Receptors, Fc / Q000096:biosynthesis
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D006023:Glycoproteins; D011961:Receptors, Fc