title: Silencing of SlFTR-c, the catalytic subunit of ferredoxin:thioredoxin reductase, induces pathogenesis-related genes and pathogen resistance in tomato plants.
abstract: As a heterodimeric protein, ferredoxin:thioredoxin reductase (FTR) catalyses the light-dependant activation of several photosynthetic enzymes. The active site of the catalytic subunit of FTR contains a redox-active disulfide and a [4Fe-4S] center. We isolated the catalytic subunit gene of FTR, designated SlFTR-c, from tomato (Solanum lycopersicum L.). SlFTR-c transcripts were detected in all tissues examined, including roots, leaves, flowers, fruits, and seeds. Interestingly, virus-induced gene silencing (VIGS) of SlFTR-c resulted in necrotic lesions with typical cell death symptoms and reactive oxygen species (ROS) production in tomato leaves. Moreover, these SlFTR-c-silenced plants displayed enhanced disease resistance against bacterial pathogens, specifically Pseudomonas syringae pv. tomato DC3000, by the induction of defense-related genes (SlPR-1, SlPR-2, SlPR-5, SlGlucA, SlChi3, and SlChi9). Taken together, it seems that SlFTR-c works as a regulator of programmed cell death (PCD) and pathogen resistance in tomato plants.
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors: Chan Ju
authors: CJ
authors: Lim
authors: Woong Bom
authors: WB
authors: Kim
authors: Bok-Sim
authors: BS
authors: Lee
authors: Ha Youn
authors: HY
authors: Lee
authors: Tae-Ho
authors: TH
authors: Kwon
authors: Jeong Mee
authors: JM
authors: Park
authors: Suk-Yoon
authors: SY
authors: Kwon
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D044224:Pseudomonas syringae; D014158:Transcription, Genetic
chemical_list: D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C021588:ferredoxin-thioredoxin reductase