Properties of HTLV-I transformed CD8+ T-cells in response to HIV-1 infection.
HIV-1 infection studies of primary CD8(+) T-cells are hampered by difficulty in obtaining a significant number of targets for infection and low levels of productive infection. Further, there exists a paucity of CD8-expressing T-cell lines to address questions pertaining to the study of CD8(+) T-cells in the context of HIV-1 infection. In this study, a set of CD8(+) T-cell clones were originated through HTLV-I transformation in vitro, and the properties of these cells were examined. The clones were susceptible to T-cell tropic strains of the virus and exhibited HIV-1 production 20-fold greater than primary CD4(+) T-cells. Productive infection resulted in a decrease in expression of CD8 and CXCR4 molecules on the surface of the CD8(+) T-cell clones and antibodies to these molecules abrogated viral binding and replication. These transformed cells provide an important tool in the study of CD8(+) T-cells and may provide important insights into the mechanism(s) behind HIV-1 induced CD8(+) T-cell dysfunction.
Gulzar
N
N
National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Ottawa, Canada.
Shroff
A
A
Buberoglu
B
B
Klonowska
D
D
Kim
J E
JE
Copeland
K F T
KF
D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015658:HIV Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D019718:Receptors, CXCR4 / Q000235:genetics / Q000276:immunology
D016827:CD8 Antigens; C527276:CXCR4 protein, human; D019718:Receptors, CXCR4