title: [Utility of microsemi LC-667CRP in point of care testing system for acute inflammatory disease].
abstract: We examined the basic performance of "Microsemi LC-667CRP" (LC-667, HORIBA, Ltd.) which has been newly developed as compact laboratory instrument capable of simultaneous measuring of complete blood count (CBC) including 3-part differentials of white blood cells (WBC) and C-reactive protein (CRP) using whole blood anticoagulated with ethylenediaminetetraacetic acid (EDTA). We found that CBC and CRP were intra-assay-reproducible (n = 10, CVs < 5.0%). They also showed the good linearity and no definite carry-over. Concerning the WBC differentials, percentage of monocytes (MON%) showed less intra-assay reproducibility compared with those of granulocytes (GRA%) and lymphocytes (LYM%). We also evaluated the correlation of values obtained by LC-667 and routinely used instruments in our institute. Concerning the CBC and WBC differentials, excellent correlations were found between LC-667CRP and XE-2100 (SYSMEX CORPORATION) except MON%. In addition, whole blood CRP as well as plasma and serum CRP measured by LC-667 also showed the good correlations with serum CRP measured by 7600 (Hitachi High Technologies Corporation). From these findings, LC-667 was revealed to produce the clinically reliable data using only 18 microL of sample volume in 4 minutes. Point of care testing (POCT) has been developed as the laboratory system performed at or near the site of patient to reduce the turn around time. Therefore, LC-667 seemed useful in POCT for the patients with acute inflammatory disease especially in pediatrics.
authors: Department of Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. inaba178@koto.kpu-m.ac.jp
authors: Tohru
authors: T
authors: Inaba
authors: Sohichi
authors: S
authors: Yuasa
authors: Hiroshi
authors: H
authors: Taniguchi
authors: Koyo
authors: K
authors: Nakashima
authors: Hiroki
authors: H
authors: Nagaoka
authors: Naohisa
authors: N
authors: Fujita
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis; D019411:Clinical Laboratory Techniques / Q000295:instrumentation*; D004492:Edetic Acid; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000175:diagnosis*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D004492:Edetic Acid