title: The Plackett-Burman design in optimization of media components for biomass production of Lactobacillus rhamnosus OXY.
abstract: The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production could be achieved by the combination of glucose (12.38 g/l), sodium pyruvate (3.15 g/l), meat extract (4.08 g/l), potassium phosphate (1.46 g/l), sodium acetate (3.65 g/l) and ammonium citrate (1.46 g/l). The validation of the predicted model carried out in bioreactor conditions confirmed the usefulness of the new medium for the culture of L. rhamnosus OXY in large scale. The optimal medium makes the culture of the probiotic bacterium L. rhamnosus OXY more cost effective.
authors: Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences, Lublin, Poland.
authors: A
authors: A
authors: Waśko
authors: Monika
authors: M
authors: Kordowska-Wiater
authors: M
authors: M
authors: Podleśny
authors: Magdalena
authors: M
authors: Polak-Berecka
authors: Z
authors: Z
authors: Targoński
authors: Agnieszka
authors: A
authors: Kubik-Komar
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology* / Q000706:statistics & numerical data; D003470:Culture Media / Q000737:chemistry*; D052201:Lacticaseibacillus rhamnosus / Q000254:growth & development* / Q000378:metabolism*; D008954:Models, Biological; D015233:Models, Statistical; D019936:Probiotics
chemical_list: D003470:Culture Media