[EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing.].
BACKGROUND
Small molecule tyrosine kinase inhibitors (TKIs), such as gefitinib and erlotinib that target the kinase domain of epidermal growth factor receptor (EGFR) are making successful progression for advanced non-small cell lung cancer patients treatment. The growing evidences revealed that EGFR exon 19 and 21 mutation status in NSCLC patients was correlated with the outcome for EGFR-TKI treatment. In this study, two methods of Real-time PCR and DNA sequencing were compared to detected EGFR exon 19 and 21 mutations.


METHODS
EGFR exon19 mutation del-E746-A750 and exon 21mutation L858R were detected by Real-time PCR and DNA sequencing in 103 NSCLC patients. Chi-square test was used to analyze the consistance.


RESULTS
There was no significant difference between the two methods. However, Real-time PCR was more convenient and sensitive compared to DNA sequencing.


CONCLUSIONS
Real-time PCR was more suitable for clinical testing than DNA sequencing.
Li
Yongwen
Y
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Liu
Hongyu
H
Li
Ying
Y
Wang
Yuli
Y
Wang
Jing
J
Chen
Jun
J
Zhou
Qinghua
Q