Montanide™ ISA 71 VG adjuvant enhances antibody and cell-mediated immune responses to profilin subunit antigen vaccination and promotes protection against Eimeria acervulina and Eimeria tenella.
The present study was conducted to investigate the immunoenhancing effects of Montanide™ ISA 71 VG adjuvant on profilin subunit antigen vaccination. Broiler chickens were immunized subcutaneously with a purified Eimeria acervulina recombinant profilin protein, either alone or mixed with ISA 71 VG, and host immune responses were evaluated. After secondary immunization, antigen-specific antibody and T-cell responses were higher in the group which received profilin plus ISA 71 VG compared with the other groups. Furthermore, body weight gains and fecal oocyst shedding were evaluated following oral challenge infection with live E. acervulina or Eimeria tenella oocysts. Vaccination with profilin plus ISA 71 VG reduced oocyst shedding compared with animals immunized with profilin alone. These results demonstrate that the recombinant profilin subunit vaccine, when given in combination with Montanide™ ISA 71 VG, augments protective immunity against E. acervulina and E. tenella.
Jang
Seung I
SI
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Lillehoj
Hyun S
HS
Lee
Sung Hyen
SH
Lee
Kyung Woo
KW
Lillehoj
Erik P
EP
Bertrand
François
F
Dupuis
Laurent
L
Deville
Sébastien
S
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D002645:Chickens / Q000254:growth & development / Q000469:parasitology*; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D016786:Eimeria tenella / Q000276:immunology; D004655:Emulsions; D005243:Feces / Q000469:parasitology; D007111:Immunity, Cellular; D008297:Male; D008353:Mannitol / Q000031:analogs & derivatives* / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology*; D010270:Parasite Egg Count / Q000662:veterinary; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D051304:Profilins / Q000276:immunology; D016052:Protozoan Vaccines / Q000276:immunology; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D022223:Vaccines, Subunit / Q000276:immunology; D015430:Weight Gain
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D004655:Emulsions; D009829:Oleic Acids; D051304:Profilins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; C477385:montanide ISA 51; D008353:Mannitol