Two types of Ca2+ channel linked to two endocytic pathways coordinately maintain synaptic transmission at the Drosophila synapse.
Endocytosis at the presynaptic terminal is initiated by Ca(2+) influx through voltage-gated Ca(2+) channels. At the Drosophila neuromuscular junction, we demonstrated two components of endocytosis linked to distinct Ca(2+) channels. A voltage-gated Ca(2+) channel blocker, (R)-(+)-Bay K8644 (R-BayK), selectively blocked one component (R-BayK-sensitive component) without affecting exocytosis, while low concentrations of La(3+) preferentially depressed the other component (La(3+) -sensitive component). In a temperature-sensitive mutant, shibire(ts), at non-permissive temperatures, dynamin clusters were found immunohistochemically at the active zone (AZ) during the R-BayK-sensitive endocytosis, while they were detected at the non-AZ during the La(3+)-sensitive endocytosis. Immunostaining of the Ca(2+) channel alpha(2)delta subunit encoded by straightjacket (stj) was found within the AZ, and a mutation in stj depressed the R-BayK-sensitive component but enhanced the La(3+) -sensitive one, indicating that the alpha(2)delta subunit is associated with the R-BayK-sensitive Ca(2+) channel. Filipin bound to the non-AZ membrane and inhibited the La(3+) -sensitive component, but not the R-BayK-sensitive one. We concluded that the R-BayK-sensitive component of endocytosis occurred at the AZ and termed this AZ endocytosis. We also concluded that the La(3+) -sensitive component occurred at the non-AZ and termed this non-AZ endocytosis. These two types of endocytosis were modulated by various drugs towards opposite directions, indicating that they were differentially regulated. During high-frequency stimulation, AZ endocytosis operated mainly in the early phase, whereas non-AZ endocytosis operated in the late phase. Thus, intense synaptic transmission is coordinately maintained by synaptic vesicle recycling initiated by Ca(2+) influx through the two types of Ca(2+) channel.
Kuromi
H
H
Faculty of Pharmacy, Iwakimeisei University, Chuoudai, Iwaki, Japan. kuromi@iwakimu.ac.jp
Ueno
K
K
Kidokoro
Y
Y
D000704:Analysis of Variance; D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology; D009469:Neuromuscular Junction / Q000502:physiology; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000502:physiology
D015220:Calcium Channels; D029721:Drosophila Proteins