title: Genome architecture marked by retrotransposons modulates predisposition to DNA methylation in cancer.
abstract: Epigenetic silencing plays an important role in cancer development. An attractive hypothesis is that local DNA features may participate in differential predisposition to gene hypermethylation. We found that, compared with methylation-resistant genes, methylation-prone genes have a lower frequency of SINE and LINE retrotransposons near their transcription start site. In several large testing sets, this distribution was highly predictive of promoter methylation. Genome-wide analysis showed that 22% of human genes were predicted to be methylation-prone in cancer; these tended to be genes that are down-regulated in cancer and that function in developmental processes. Moreover, retrotransposon distribution marks a larger fraction of methylation-prone genes compared to Polycomb group protein (PcG) marking in embryonic stem cells; indeed, PcG marking and our predictive model based on retrotransposon frequency appear to be correlated but also complementary. In summary, our data indicate that retrotransposon elements, which are widespread in our genome, are strongly associated with gene promoter DNA methylation in cancer and may in fact play a role in influencing epigenetic regulation in normal and abnormal physiological states.
authors: Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA. mestecio@mdanderson.org
authors: Marcos R H
authors: MR
authors: Estécio
authors: Juan
authors: J
authors: Gallegos
authors: Céline
authors: C
authors: Vallot
authors: Ryan J
authors: RJ
authors: Castoro
authors: Woonbok
authors: W
authors: Chung
authors: Shinji
authors: S
authors: Maegawa
authors: Yasuhiro
authors: Y
authors: Oki
authors: Yutaka
authors: Y
authors: Kondo
authors: Jaroslav
authors: J
authors: Jelinek
authors: Lanlan
authors: L
authors: Shen
authors: Helge
authors: H
authors: Hartung
authors: Peter D
authors: PD
authors: Aplan
authors: Bogdan A
authors: BA
authors: Czerniak
authors: Shoudan
authors: S
authors: Liang
authors: Jean-Pierre J
authors: JP
authors: Issa
mesh_terms: D045744:Cell Line, Tumor; D019175:DNA Methylation; D057890:Epigenomics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D015894:Genome, Human; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D009369:Neoplasms / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms
chemical_list: D018626:Retroelements