title: Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms.
abstract: A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5 mM [Fe(CN)(6)](3-/4-), obtaining a detection limit of 105 pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications.
authors: Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, CIN2 (CSIC), Campus UAB, Barcelona, Spain.
authors: Pinar
authors: P
authors: Kara
authors: Alfredo
authors: A
authors: de la Escosura-Muñiz
authors: Marisa
authors: M
authors: Maltez-da Costa
authors: Maria
authors: M
authors: Guix
authors: Mehmet
authors: M
authors: Ozsoz
authors: Arben
authors: A
authors: Merkoçi
mesh_terms: D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000648:ultrastructure; D011506:Proteins / Q000032:analysis*; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D037742:Nanotubes, Carbon; D011506:Proteins; C081676:thrombin aptamer; D013917:Thrombin