Immunohistochemical expression of procaspase-3 and its clinical significance in childhood non-Hodgkin lymphomas.
Previous studies have shown differences in expression levels of apoptosis regulatory proteins in non-Hodgkin lymphomas (NHLs) and indicated the correlation of procaspase-3 (proC-3) and caspase-3 activation to the response of chemotherapy. We investigated whether proC-3 expression in tumor biopsies of childhood NHLs is related to clinical outcome. Formalin-fixed paraffin-embedded tissues from 58 children with NHL were evaluated for proC-3 expression by immunochemistry analysis. The study included 20 cases of Burkitt lymphoma, 7 cases of diffuse large B-cell lymphoma, 18 cases of anaplastic large cell lymphoma (ALCL), and 13 cases of precursor lymphoblastic lymphoma. The highest expression level of proC-3 was observed in ALCL. In the multivariate analysis the higher clinical stage of disease and higher expression level of proC-3 were independent and appear to be significant prognostic factors of treatment failure. Our results suggest that the high expression level of proC-3 may be a powerful independent predictor of response to chemotherapy and progression-free survival in childhood NHLs.
Wrobel
Grazyna
G
Department of Paediatric Bone Marrow Transplantation, Medical University, Wroclaw, Poland. wrobel.wroc@wp.pl
Maldyk
Jadwiga
J
Kazanowska
Bernarda
B
Rapala
Malgorzata
M
Maciejka-Kapuscinska
Lucyna
L
Chaber
Radosław
R
D000293:Adolescent; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis*; D053148:Caspase 3 / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D019008:Drug Resistance, Neoplasm / Q000502:physiology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis
D014408:Biomarkers, Tumor; C505407:CASP3 protein, human; D053148:Caspase 3