Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice.
Interleukin (IL)-10 exerts potent anti-inflammatory effects by suppression of both T-help (Th) 1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study was performed to examine whether IL-10 plasmid DNA could suppress development of atopic dermatitis (AD)-like skin lesions in NC/Nga mice, as an initial step towards the development of an appliance for use in dogs with AD. Intradermal injection of IL-10 plasmid DNA markedly inhibited the development of AD-like skin lesions, as evidenced by a marked decrease in skin symptoms and reduced inflammation within the skin lesions. Efficacy was confirmed by significant decreases in eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Moreover, relative mRNA expression levels of IL-4 and interferon-gamma in the skin lesions of mice injected with IL-10 plasmid DNA were also decreased compared with those of control mice. Of note, higher serum IL-10 levels in mice injected with IL-10 plasmid DNA were maintained compared with those in control mice. Taken together, the results indicate that IL-10 plasmid DNA can suppress the development of AD-like skin lesions by suppressing both Th1 and Th2 cell responses. Beneficial effects of IL-10 plasmid DNA may be expected in dogs with AD.
Jung
Bock Gie
BG
College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
Cho
Sun Ju
SJ
Ko
Jae Hyung
JH
Lee
Bong Joo
BJ
D000818:Animals; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D003876:Dermatitis, Atopic / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use*; D051379:Mice; D008817:Mice, Mutant Strains; D010957:Plasmids / Q000235:genetics / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000302:isolation & purification; D018709:Statistics, Nonparametric; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D017931:DNA Primers; D016753:Interleukin-10