title: Isolation and purification of two bacteriocins 3D produced by Enterococcus faecium with inhibitory activity against Listeria monocytogenes.
abstract: Strain 3D, isolated from fermented traditional Moroccan dairy product, and identified as Enterococcus faecium, was studied for its capability to produce two bacteriocins acting against Listeria monocytogenes. Bacteriocins 3 Da and 3Db were heat stable inactivated by proteinase K, pepsin, and trypsin but not when treated with catalase. The evidenced bacteriocins were stable in a wide pH range from 2 to 11 and bactericidal activity was kept during storage at 4°C. However, the combination of temperature and pH exhibited a stability of the bacteriocins. RP-HPLC purification of the anti-microbial compounds shows two active fractions eluted at 16 and 30.5 min, respectively. Mass spectrometry analysis showed that E. faecium 3D produce two bacteriocins Enterocin 3 Da (3893.080 Da) and Enterocin 3Db (4203.350 Da). This strain is food-grade organism and its bacteriocins were heat-stable peptides at basic, neutral, and acid pH: such bacteriocins may be of interest as food preservatives.
authors: Laboratory of Physiology and Molecular Genetics (PGM), Faculty of Sciences, Department of Biology, Aîn chock, University Hassan II, Casablanca, Morocco.
authors: Kaoutar
authors: K
authors: Bayoub
authors: Ilham
authors: I
authors: Mardad
authors: Emna
authors: E
authors: Ammar
authors: Aurelio
authors: A
authors: Serrano
authors: Abdelaziz
authors: A
authors: Soukri
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000378:metabolism*; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects*; D013058:Mass Spectrometry; D050296:Microbial Viability / Q000187:drug effects; D008970:Molecular Weight; D055550:Protein Stability; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins