Dumbbell-like Au-Fe3O4 nanoparticles as label for the preparation of electrochemical immunosensors.
In this work, we developed a novel kind of label based on dumbbell-like Au-Fe3O4 nanoparticles (NPs) for the preparation of electrochemical immunosensor for the detection of cancer biomarker prostate specific antigen (PSA). The signal amplification strategy, using the synergetic effect present in Au-Fe3O4 to increase the reduction ability of the NPs toward H2O2, improves the sensitivity and detection limit of the immunosensor. With primary anti-PSA antibody (Ab1) immobilized onto graphene surface and secondary anti-PSA antibody (Ab2) adsorbed onto the Au of the Au-Fe3O4 NPs, the immunosensor prepared through a sandwich structure displays a wide linear range (0.01-10 ng/mL), low detection limit (5 pg/mL), good reproducibility and stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules.
Wei
Qin
Q
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Xiang
Zhi
Z
He
Jing
J
Wang
Gaolei
G
Li
He
H
Qian
Zhiyong
Z
Yang
Minghui
M
D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D010316:Particle Size; D017430:Prostate-Specific Antigen / Q000097:blood*; D013194:Staining and Labeling / Q000379:methods
D058185:Magnetite Nanoparticles; D006046:Gold; D006861:Hydrogen Peroxide; D017430:Prostate-Specific Antigen