Electrochemical and Laser Deposition of Silver for Use in Metal-Enhanced Fluorescence.
We describe two reagentless methods of silver deposition for metal-enhanced fluorescence. Silver was deposited on glass positioned between two silver electrodes with a constant current in pure water. Illumination of the glass between the electrodes resulted in localized silver deposition. Alternatively, silver was deposited on an Indium Tin Oxide cathode, with a silver electrode as the anode. Both types of deposited silver produced a 5-18-fold increase in the fluorescence intensity of a nearby fluorophore, indocyanine green (ICG). Additionally, the photostability of ICG was dramatically increased by proximity to the deposited silver. These results suggest the use of silver deposited from pure water for surface-enhanced fluorescence, with potential applications in surface assays and lab-on-a-chip-based technologies, which ideally require highly fluorescent photostable systems.
Geddes
Chris D
CD
Institute of Fluorescence and Center for Fluorescence Spectroscopy, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201, Department of Chemistry, Roberts Wesleyan College, 2301 Westside Drive, Rochester, New York 14624, and Center for Biomolecular Science and Engineering (Code 6900), Naval Research Laboratory, Washington, D.C. 20375.
Parfenov
Alexandr
A
Roll
David
D
Fang
Jiyu
J
Lakowicz
Joseph R
JR