Strip-based amperometric detection of myeloperoxidase.
The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18 U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.
Windmiller
Joshua R
JR
Department of NanoEngineering, University of California-San Diego, La Jolla, CA 92093, United States.
Chinnapareddy
Soujanya
S
Santhosh
Padmanabhan
P
Halámek
Jan
J
Chuang
Min-Chieh
MC
Bocharova
Vera
V
Tseng
Ta-Feng
TF
Chou
Tzu-Yang
TY
Katz
Evgeny
E
Wang
Joseph
J
D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D009195:Peroxidase / Q000032:analysis*; D011934:Reagent Strips
D011934:Reagent Strips; D009195:Peroxidase