title: In situ muGISAXS: II. Thaumatin crystal growth kinetic.
abstract: The formation of thaumatin crystals by Langmuir-Blodgett (LB) film nanotemplates was studied by the hanging-drop technique in a flow-through cell by synchrotron radiation micrograzing-incidence small-angle x-ray scattering. The kinetics of crystallization was measured directly on the interface of the LB film crystallization nanotemplate. The evolution of the micrograzing-incidence small-angle x-ray scattering patterns suggests that the increase in intensity in the Yoneda region is due to protein incorporation into the LB film. The intensity variation suggests several steps, which were modeled by system dynamics based on first-order differential equations. The kinetic data can be described by two processes that take place on the LB film, a first, fast, process, attributed to the crystal growth and its detachment from the LB film, and a second, slower process, attributed to an unordered association and conversion of protein on the LB film.
authors: European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble Cedex, France.
authors: Ronald
authors: R
authors: Gebhardt
authors: Eugenia
authors: E
authors: Pechkova
authors: Christian
authors: C
authors: Riekel
authors: Claudio
authors: C
authors: Nicolini
mesh_terms: D003460:Crystallization; D007700:Kinetics; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D013997:Time Factors; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D010940:Plant Proteins; C003427:thaumatin protein, plant