Blood group genotyping by high-throughput DNA analysis applied to 356 reagent red blood cell samples.
BACKGROUND
DNA analysis for the prediction of blood group antigen expression has broad implications in transfusion medicine. It may be of particular interest especially to detect variants, when antigen expression is weak or altered. The use of high-throughput DNA analysis has never been applied to donors whose red blood cells (RBCs) are selected for reagent RBCs. The aim of this study was to analyze the concordance between the serologic phenotype and that predicted from DNA analysis in panel donors, to determine the benefit of the use of DNA analysis in reagent RBC selection strategy.


METHODS
The "Panel National de Référence du Centre National de Référence sur les Groupes Sanguins" is a reference reagent mainly used for antibody identification. DNA genotyping of 356 panel donors was performed with BeadChips (human erythrocyte antigen v1.2 BeadChips, BioArray Solutions). The comparison between serologic phenotype and that predicted from DNA analysis held on 8876 paired results obtained from 10 blood group systems and 25 antigens.


RESULTS
A 99.95% concordance was observed. Discrepancies in four cases (RH, KEL, LU, and DO systems) were analyzed. Genotyping precisions on the Duffy system were of particular interest. No new rare blood group was observed.


CONCLUSIONS
Systematic DNA analysis of panel donors should unquestionably change the management of reagent RBC selection. The notion of "antigens in double dose" should evolve regarding data obtained from DNA analysis, allowing an improved quality of reagent RBCs for antibody screening and identification.
Kappler-Gratias
Sandrine
S
Institut National de la Transfusion Sanguine, Centre National de Référence sur les Groupes Sanguins-INSERM U665, Paris, France. skappler@ints.fr
Peyrard
Thierry
T
Beolet
Marylise
M
Amiranoff
Denise
D
Menanteau
Cécile
C
Dubeaux
Isabelle
I
Rouger
Philippe
P
Cartron
Jean-Pierre
JP
Le Pennec
Pierre-Yves
PY
Pham
Bach-Nga
BN
D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods*
D001789:Blood Group Antigens