Serological diagnosis of brucellosis.
OBJECTIVE
To present a review and to describe the most widely used laboratory tests for serology diagnosis of brucellosis along with their pros and cons.


METHODS
Review the recent literature on brucellosis serology diagnostic tests. The choice of the testing strategy depends on the prevailing brucellosis epidemiological situation and the goal of testing.


RESULTS
The 'gold standard' for the diagnosis of brucellosis is isolation and identification of the causative bacterium, a member of Brucella sp. Isolation of Brucella sp. requires high security laboratory facilities (biological containment level 3), highly skilled personnel, an extended turnaround time for results and it is considered a hazardous procedure. Hence brucellosis is generally diagnosed by detection of an elevated level of antibody in serum or other body fluid. This is a presumptive diagnosis as other microorganisms and perhaps environmental factors can also cause increased antibody levels.


CONCLUSIONS
A large number of serological tests for brucellosis have been devised over the 100+ years since its initial isolation, starting with a simple agglutination test and progressing to sophisticated primary binding assays available today. However, no test devised to date is 100% accurate so generally serological diagnosis consists of testing sera by several tests, usually a screening test of high sensitivity, followed by a confirmatory test of high specificity.
Nielsen
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Ottawa Laboratories (Fallowfield), Canadian Food Inspection Agency, Nepean, Ontario, Canada.
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D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis; D002002:Brucella / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis*; D006801:Humans; D012698:Serologic Tests / Q000379:methods*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial