title: Identification and validation of specific methylation profile in bile for differential diagnosis of malignant biliary stricture.
abstract: OBJECTIVE
  This study was aimed to identify the specific methylation profile in bile specimens of pancreaticobillary diseases for differential diagnosis of malignant biliary stricture.
  
  
  METHODS
  In a total of 80 bile specimens from pancreaticobillary diseases, the methylation status of 19 tumor suppressor genes were analyzed by methylation-specific PCR and the methylation index (MI) were compared between the malignant and benign groups.
  
  
  RESULTS
  Methylation of DKK3, p16, SFRP2, DKK2, NPTX2 and ppENK were more frequently detected in the bile of malignant biliary strictures than benign patients. When setting MI 0.5 as the threshold, this 6-gene panel could distinguish the malignant biliary stricture with a high sensitivity, specificity and accuracy (77.27%, 77.78% and 77.50%, respectively).
  
  
  CONCLUSIONS
  The methylation profile including 6 specific genes in bile may be a promising biomarker for differential diagnosis between malignant and benign biliary strictures.
authors: Third Central Clinical College of Tianjin Medical University, Tianjin 300170, China.
authors: Ye
authors: Y
authors: Zhang
authors: Bin
authors: B
authors: Yang
authors: Zhi
authors: Z
authors: Du
authors: Ying-Tang
authors: YT
authors: Gao
authors: Yi-Jun
authors: YJ
authors: Wang
authors: Xiang
authors: X
authors: Jing
authors: Tong
authors: T
authors: Bai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001646:Bile / Q000378:metabolism*; D001649:Bile Duct Diseases / Q000175:diagnosis* / Q000235:genetics*; D003251:Constriction, Pathologic; D019175:DNA Methylation / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results