title: Genotyping of Aeromonas hydrophila by box elements.
abstract: PCR-based DNA fingerprinting techniques were evaluated to genotype eight diseased, particularly normal and environmental isolates of Aeromonas hydrophila. PCR-based fingerprinting method has an advantage of having repetitive sequence also called Box elements that are interspersed throughout the genome in diverse bacterial species. The BOX-PCR fingerprinting technique was evaluated for the discrimination of different isolates of A. hydrophila. All the studied isolates have shown major banding patterns ranged from 500-3000 bp. These finding could be advantageous to investigate the strain level specific fingerprints of A. hydrophila as potential genotypic markers.
authors: National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow-226002, India. vijaisingh15@gmail.com
authors: Vijai
authors: V
authors: Singh
authors: Dharmendra Kumar
authors: DK
authors: Chaudhary
authors: Indra
authors: I
authors: Mani
authors: Pallavi
authors: P
authors: Somvanshi
authors: Gaurav
authors: G
authors: Rathore
authors: Neeraj
authors: N
authors: Sood
mesh_terms: D016980:Aeromonas hydrophila / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial