title: Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal reconstruction and regeneration.
abstract: We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma + epithelium), using primary human keratocytes and limbal stem cell derived human keratinocytes. On the surface of these hemi-corneas, a well-differentiated epithelium was formed, as determined both histologically and ultrastructurally and by the expression of characteristic markers. Within the stroma, the keratocytes aligned with the directions of the fibrils in the scaffold and synthesized a new extracellular matrix with typical collagen markers and small, uniform diameter fibrils. Finally, in vivo experiments using a rabbit model showed that these orthogonally oriented multi-layer scaffolds could be used to repair the anterior region of the stroma, leading to re-epithelialization and recovery of both transparency and ultrastructural organization.
authors: Banque de Cornées des Hospices Civils de Lyon, 69437 Lyon cedex 3, France.
authors: Nicolas
authors: N
authors: Builles
authors: Hélène
authors: H
authors: Janin-Manificat
authors: Marilyne
authors: M
authors: Malbouyres
authors: Virginie
authors: V
authors: Justin
authors: Marie-Rose
authors: MR
authors: Rovère
authors: Graziella
authors: G
authors: Pellegrini
authors: Jim
authors: J
authors: Torbet
authors: David J S
authors: DJ
authors: Hulmes
authors: Carole
authors: C
authors: Burillon
authors: Odile
authors: O
authors: Damour
authors: Florence
authors: F
authors: Ruggiero
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D003315:Cornea / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D020007:Implants, Experimental; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D008280:Magnetics; D008297:Male; D011817:Rabbits; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003094:Collagen