Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice.
This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.
Ahn
Eun Hee
EH
Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
Kim
Dae Won
DW
Kang
Hye Won
HW
Shin
Min Jae
MJ
Won
Moo Ho
MH
Kim
Joon
J
Kim
Dong Joon
DJ
Kwon
Oh-Shin
OS
Kang
Tae-Cheon
TC
Han
Kyu Hyung
KH
Park
Jinseu
J
Eum
Won Sik
WS
Choi
Soo Young
SY
D000818:Animals; D003872:Dermatitis / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D004487:Edema / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D005822:Genetic Vectors / Q000627:therapeutic use; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010447:Peptide Hydrolases / Q000235:genetics* / Q000627:therapeutic use; D010940:Plant Proteins / Q000235:genetics* / Q000627:therapeutic use; D012269:Ribosomal Proteins / Q000235:genetics* / Q000627:therapeutic use; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014161:Transduction, Genetic / Q000379:methods
D018836:Inflammation Mediators; D010940:Plant Proteins; D012269:Ribosomal Proteins; C082041:YRPS3 protein, Petunia hybrida; C044152:ribosomal protein S3; D010447:Peptide Hydrolases; C058973:peptidase-1 (Pleurodeles); D013755:Tetradecanoylphorbol Acetate