Dynamic axial stabilization of counter-propagating beam-traps with feedback control.
Optical trapping in a counter-propagating (CP) beam-geometry provides unique advantages in terms of working distance, aberration requirements and intensity hotspots. However, its axial performance is governed by the wave propagation of the opposing beams, which can limit the practical geometries. Here we propose a dynamic method for controlling axial forces to overcome this constraint. The technique uses computer-vision object tracking of the axial position, in conjunction with software-based feedback, for dynamically stabilizing the axial forces. We present proof-of-concept experiments showing real-time rapid repositioning coupled with a strongly enhanced axial trapping for a plurality of particles of varying sizes. We also demonstrate the technique's adaptability for real-time reconfigurable feedback-trapping of a dynamically growing structure that mimics a continuously dividing cell colony. Advanced implementation of this feedback-driven approach can help make CP-trapping resistant to a host of perturbations such as laser fluctuations, mechanical vibrations and other distortions emphasizing its experimental versatility.
Tauro
Sandeep
S
DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
Bañas
Andrew
A
Palima
Darwin
D
Glückstad
Jesper
J
D004867:Equipment Design; D005246:Feedback; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D007909:Lenses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D052898:Optical Tweezers; D012984:Software; D014732:Vibration