Development of the fingerprint for the quality of Radix Linderae through ultra-pressure liquid chromatography-photodiode array detection/electrospray ionization mass spectrometry.
An ultra-pressure LC (UPLC) method has been developed and validated for the quality evaluation of a traditional Chinese medicine (Radix Linderae) by chemical fingerprint analysis with chromatograms collected at two wavelengths (260 and 320 nm). Eleven characteristic peaks in the fingerprints were identified by comparing their retention times, UV spectra and ESI-MS/MS data with those of the reference substances or the data in the literatures. Both correlation coefficient of similarities in chromatograms and relative peak areas of common peaks were calculated for quality expression of the Radix Linderae samples collected from different areas in China. The results showed high variation of relative peak area and correlation coefficients among the samples collected from various habitats, which indicated that the quality consistency of Radix Linderae is still a problem worthy of serious concern.
Wu
Yong-jiang
YJ
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, P. R. China.
Zheng
Yun-liang
YL
Luan
Lian-jun
LJ
Liu
Xue-song
XS
Han
Zheng
Z
Ren
Yi-ping
YP
Gan
Li-she
LS
Zhou
Chang-xin
CX
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006801:Humans; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012997:Solvents