title: Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells.
abstract: We analyzed the mRNA diversity of genes after inducing neuronal differentiation in human NT2 teratocarcinoma cells using all-trans retinoic acid (RA). DNA microarray analyses of cells treated with RA identified 358 RA-responsive genes. mRNA diversity analysis revealed that 274 genes produced multiple protein-coding transcripts by alternative splicing. Among these 274 genes, we chose 26 genes that showed AS in their C-terminus and 12 transcription factor genes for further analysis. By using transcript-specific primers, we performed quantitative real-time PCR analysis to examine the expression profiles of all the protein-coding transcripts. Consequently, we identified genes which showed different RA-induced changes in the expression of their protein-coding transcripts.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors: Ai
authors: A
authors: Wakamatsu
authors: Jun-ichi
authors: J
authors: Imai
authors: Shinya
authors: S
authors: Watanabe
authors: Takao
authors: T
authors: Isogai
mesh_terms: D017398:Alternative Splicing; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D055495:Neurogenesis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014212:Tretinoin / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D014157:Transcription Factors; D014212:Tretinoin