Use of bovine hydroxyapatite with or without biomembrane in sinus lift in rabbits: histopathologic analysis and immune expression of core binding factor 1 and vascular endothelium growth factor.
OBJECTIVE
Considering the clinical discussion on the necessity of using a barrier membrane in the osteotomy area of sinus lift procedures to prevent fibrous tissue formation in this area and as a physical limit, the aim of this study was to analyze and compare the use of bovine hydroxyapatite (HA) with and without a biologic membrane by histopathologic analysis and immune expression of core binding factor 1 and vascular endothelium growth factor in the sinus lift in rabbits.


METHODS
Sixteen male rabbits underwent bilateral sinus lift procedures and were divided into 2 groups according to the sinus filling material: group 1 received bovine HA (Bio-Oss; Geistlich Pharma AG, Wohlhusen, Switzerland) and group 2 received bovine HA and a nonporous polytetrafluorethylene membrane. All groups were sacrificed after 7, 14, 30, and 60 days for microscopic, histomorphometric, and immunohistochemical analyses.


RESULTS
Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft tissue, and the remaining material were analyzed by histomorphometric analysis. No statistically significant differences (P > .05) were detected between groups for all periods analyzed. In addition, no remarkable differences were noticed in core binding factor 1 or vascular endothelium growth factor immune expression.


CONCLUSIONS
Taken together, these results show that using a biologic membrane does not improve bone repair induced by bovine HA, as shown by histopathologic and immunohistochemical analyses.
De Souza Nunes
Leandro Soeiro
LS
Department of Health Sciences, Discipline of Oral and Maxillofacial Surgery, School of Dentistry, Sagrado Coração University, Bauru, SP, Brazil.
De Oliveira
Renato Victor
RV
Holgado
Leandro Andrade
LA
Nary Filho
Hugo
H
Ribeiro
Daniel Araki
DA
Matsumoto
Mariza Akemi
MA
D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001857:Bone Matrix / Q000637:transplantation; D018786:Bone Substitutes / Q000627:therapeutic use*; D002417:Cattle; D003238:Connective Tissue / Q000473:pathology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis*; D017886:Durapatite / Q000627:therapeutic use*; D006097:Granulation Tissue / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008437:Maxilla / Q000473:pathology / Q000601:surgery*; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery*; D008567:Membranes, Artificial; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis / Q000502:physiology; D010027:Osteotomy / Q000379:methods; D011138:Polytetrafluoroethylene; D011817:Rabbits; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D008567:Membranes, Artificial; D008903:Minerals; D042461:Vascular Endothelial Growth Factor A; D011138:Polytetrafluoroethylene; D017886:Durapatite