[Synthesis and identification of artificial antigens for sulfaguanidine and sulfathiazole].
OBJECTIVE
To prepare artificial antigens of sulfaguanidine and sulfathiazole for detecting sulfaguanidine and sulfathiazole in foods of animal origin.


METHODS
The artificial antigens were synthesized by the diazotization methods of coupling sulfaguanidine and sulfathiazole with bovine serum albumin (BSA), and the coupling ratio was 10 : 1 and 9 : 1 respectively. The anti-serum of Balb/c mice was obtained after 5 injections of artificial antigens and the antibody titles were detected by iELISA method.


RESULTS
The artificial antigens were identified by infrared spectra, ultraviolet spectra and immunized experimental animals.


CONCLUSIONS
The antigens for sulfaguanidine and sulfathiazole were acquired successfully. The results have laid basis for the specific immunoassay of sulfaguanidine and sulfathiazole.
Cui
Yanhua
Y
School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. yhcui@hit.edu.cn
Zhang
Hailing
H
ma
Ying
Y
Dai
Cuihong
C
Cui
Jie
J
D000818:Animals; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D004350:Drug Residues / Q000032:analysis; D005260:Female; D005504:Food Analysis / Q000379:methods; D006241:Haptens / Q000737:chemistry; D007114:Immunization; D007118:Immunoassay / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013414:Sulfaguanidine / Q000032:analysis / Q000276:immunology*; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000032:analysis / Q000276:immunology*
D000941:Antigens; D006241:Haptens; D013432:Sulfathiazoles; D013414:Sulfaguanidine; D012710:Serum Albumin, Bovine; D000077589:Sulfathiazole