Antiangiogenetic effects of 4 varieties of grapes in vitro.
The purpose of this study was to investigate the inhibitory effects of grapes on the human umbilical vein endothelial (HUVE) cells' capillary tube formation and matrix metalloproteinase-2 (MMP-2) expression secreted into the medium. Four different grape varieties (Concord, Niagara, Chardonnay, and Pinot Noir) were extracted using 80% acetone and the extracts were stored at -80 degrees C. The total amount of phenolics and flavonoids for each of the 4 grape varieties were determined by spectrophotometry. Grape extracts were co-cultured with HUVE cells on Matrigel and inhibitory effects on tube formation were observed under a microscope. The inhibitory effects of grape extracts on MMP-2 expression were examined by zymogram. All 4 grape varieties inhibited the tube formation of HUVE cells in a dose-dependent manner on Matrigel. Except for Chardonnay, the other 3 grape varieties completely inhibited secretion of MMP-2 at 20 mg/mL. There was a significant positive relationship between the total phenolics and flavonoids and antiangiogenetic activities. The grapes tested have the potential to inhibit angiogenesis mainly by their phenolics and flavonoids contents, which partly contribute to their cancer chemopreventive efficacy.
Liu
Ming
M
Treatment Center of Oncology, Fourth Hospital of Harbin Medical Univ., Harbin, P.R. China. mliu35@yahoo.com.cn
Liu
Rui-Hai
RH
Song
Bing-Bing
BB
Li
Chun-Feng
CF
Lin
Luo-Qiang
LQ
Zhang
Chun-Peng
CP
Zhao
Jin-Lu
JL
Liu
Jia-Ren
JR
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology; D054443:Cell Migration Assays; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D009994:Osmolar Concentration; D010636:Phenols / Q000032:analysis; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D055571:Secretory Pathway / Q000187:drug effects; D027843:Vitis / Q000737:chemistry*
D020533:Angiogenesis Inhibitors; D016588:Anticarcinogenic Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2