Chemical composition and antioxidant and antimycobacterial activities of Bromelia balansae (Bromeliaceae).
Bromelia balansae (Family Bromeliaceae) is a medicinal plant commonly used in the central region of Brazil as a cough syrup and also eaten roasted. The methanolic extract of ripe fruits was analyzed by chromatographic methods and spectrometrically. Four glycoside flavonols were isolated: kaempferol-3-O-α-L-rhamnopyranoside (1), kaempferol-3-O-α-L-rhamnopyranosyl-(1-->6)-ß-D-glucopyranoside (2), quercetin-3-O-α-L-rhamnopyranosyl-(1-->6)-ß-d-glucopyranoside (3), and kaempferol 3,7-di-O-α-L-rhamnopyranoside (4). The resazurin microtiter assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis. The results showed a moderate activity of the methanolic extract with a minimal inhibitory concentration of 128 μg/mL. Antioxidant activity was evaluated as free radical scavenging capacity and inhibition of peroxidation. Free radical scavenging capacity was assessed by measuring the scavenging activity of methanolic extract and methanolic fraction on 2,2-diphenyl-1-picrylhydrazyl radical. The methanolic extract showed low values of antioxidant activities, whereas the methanolic fraction exhibited free radical scavenging activity ranging from 20.2% to 91.1%, and the inhibition of peroxidation values ranging from 5.6% to 27.5%. This is the first chemical study reported in the literature about this species.
Coelho
Roberta Gomes
RG
Department of Chemistry, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil. robertacoelho@yahoo.com.br
Honda
Neli Kika
NK
Vieira
Maria do Carmo
Mdo C
Brum
Rosenei Louzada
RL
Pavan
Fernando Rogério
FR
Leite
Clarice Queico Fujimura
CQ
Cardoso
Claudia Andréa Lima
CA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D031231:Bromelia / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D000432:Methanol