Mapping of sequence-specific markers and loci controlling preharvest sprouting and alpha-amylase activity in rye (Secale cereale L.) on the genetic map of an F2 (S120&times;S76) population.
Location of the loci that control preharvest sprouting and alpha-amylase activity in rye was studied based on intercross S120&times;S76, consisting of 110 genotypes of F2 and F3 progenies. The genetic map currently consists of 141 loci distributed in 11 linkage groups, covering a distance of 506.4 cM, and was enriched during this study with 24 sequence-specific markers (7 SCARs, 7 SSRs, and 10 STSs). The extended map was applied for composite interval mapping of the loci controlling preharvest sprouting and &alpha;-amylase activity, revealing 3 significant QTLs for preharvest sprouting, located on chromosomes 3R, 5R and 6R (in 1999), and one QTL for &alpha;-amylase activity found on chromosome 2R (in 2000).
Myskow
B
B
Department of Plant Genetics, Breeding and Biotechnology, West-Pomeranian University of Technology, Szczecin, Poland. bmyskow@zut.edu.pl
Stojalowski
S
S
Milczarski
P
P
Masojc
P
P
D000383:Agriculture; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers; D005828:Genetics, Population; D018525:Germination / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012434:Secale / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D000516:alpha-Amylases / Q000235:genetics*
D005819:Genetic Markers; D000516:alpha-Amylases