title: [Correlation between ethambutol-resistance and embB gene mutation in Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To analyze the characteristics of embB gene mutation in ethambutol-resistant Mycobacterium tuberculosis (M. TB) strains isolated from patients in Xi'an for establishing a rapid method to detect the mutation of embB gene in M. TB.
  
  
  METHODS
  The embB gene of 104 Mycobacterium tuberculosis isolates was detected by PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP).
  
  
  RESULTS
  Taking strain H37Rv as a control, the SSCP and RFLP profiles of embB of 35 drug-sensitive strains in 104 Mycobacterium tuberculosis isolates were normal. The display of SSCP profiles in 39 of 69 (56.52%) ethambutol-resistant isolates and the display of RFLP profiles in 19 of 69 (27.54%) ethambutol-resistant isolates were abnormal.
  
  
  CONCLUSIONS
  The EMB-resistance of M. TB strains isolated from patients in Xi'an is related to the mutation of embB gene, PCR-SSCP and PCR-RFLP are sensitive methods for detecting ethambutol-resistance of M. tuberculosis.
authors: Department of Immunology and Pathogen Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. liviy@163.com
authors: Wei
authors: W
authors: Li
authors: Xin
authors: X
authors: Xue
authors: Yonglie
authors: Y
authors: Chu
authors: Yi
authors: Y
authors: Qiu
authors: Juan
authors: J
authors: Song
authors: Jianwu
authors: J
authors: Zheng
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004977:Ethambutol / Q000494:pharmacology*; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics
chemical_list: D004977:Ethambutol; C540269:EmbB protein, Mycobacterium tuberculosis; D010430:Pentosyltransferases