title: Effects of time point measurement on the reconstruction of gene regulatory networks.
abstract: With the availability of high-throughput gene expression data in the post-genomic era, reconstruction of gene regulatory networks has become a hot topic. Regulatory networks have been intensively studied over the last decade and many software tools are currently available. However, the impact of time point selection on network reconstruction is often underestimated. In this paper we apply the Dynamic Bayesian network (DBN) to construct the Arabidopsis gene regulatory networks by analyzing the time-series gene microarray data. In order to evaluate the impact of time point measurement on network reconstruction, we deleted time points one by one to yield 11 distinct groups of incomplete time series. Then the gene regulatory networks constructed based on complete and incomplete data series are compared in terms of statistics at different levels. Two time points are found to play a significant role in the Arabidopsis gene regulatory networks. Pathway analysis of significant nodes revealed three key regulatory genes. In addition, important regulations between genes, which were insensitive to the time point measurement, were also identified.
authors: Center for Systems Biology, Soochow University, Suzhou, Jiangsu, China.
authors: Wenying
authors: W
authors: Yan
authors: Huangqiong
authors: H
authors: Zhu
authors: Yang
authors: Y
authors: Yang
authors: Jiajia
authors: J
authors: Chen
authors: Yuanyuan
authors: Y
authors: Zhang
authors: Bairong
authors: B
authors: Shen
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D013997:Time Factors