title: Immunocytological analysis of chromatin in isolated nuclei.
abstract: All cells in a multicellular organism have the same genetic constitution, yet their appearance and function may differ enormously, due to differences in the nuclear program. Central in the establishment of this cell diversity are epigenetic marks, which are largely based on covalent modifications of histones and methylated cytosine residues in the DNA sequence. The study of these epigenetic factors in individual cells requires the microscopic visualization of chromatin components. Here we describe a number of protocols to study chromatin in isolated nuclei.
authors: Laboratory of Genetics, Wageningen University, Wageningen, Netherlands.
authors: Penka
authors: P
authors: Pavlova
authors: Federico
authors: F
authors: Tessadori
authors: Hans J
authors: HJ
authors: de Jong
authors: Paul
authors: P
authors: Fransz
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000032:analysis; D002467:Cell Nucleus / Q000235:genetics / Q000648:ultrastructure*; D002843:Chromatin; D019175:DNA Methylation; D018744:DNA, Plant / Q000032:analysis*; D005557:Formaldehyde; D006570:Heterochromatin; D007150:Immunohistochemistry / Q000379:methods*
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D018744:DNA, Plant; D006570:Heterochromatin; D005557:Formaldehyde