Multi-parameter detection of diabetes mellitus on multichannel poly(dimethylsiloxane) analytical chips coupled with nanoband microelectrode arrays.
This article demonstrates a novel method for multi-parameter detection of diabetes mellitus. We propose an approach for fabrication of a 3-D metal films array with gold and copper using electroless deposition technique on PDMS substrate. The obtained PDMS slices containing metal films are superimposed layer by layer as a sandwich structure to form 3-D metal films array. The cross-sections of the array could be used as nanoband array electrochemical detectors, which are further integrated with a multichannel microchip for simultaneously detecting multi-parameter of diabetes mellitus, including glucose and metabonomics of diabetes containing aldehyde compounds (glyoxal and methylglyoxal) and short organic acids (lactate, urate and 2-hydroxybutyrate). Under optimized separation and detection conditions, glucose, aldehyde compounds and short organic acids respond linearly in the concentration range of 10-2000, 1-500 and 5-600 μM, with the LODs of 4, 0.5 and 3 μM for glucose, aldehyde compounds and short organic acids, respectively. This system is successfully employed to detect these compounds in serums. This study reveals that the electrochemical array detectors with different materials integrated with multichannel microchip provide a flexible and inexpensive approach for routine, simultaneous and direct detection of some metabolites in metabonomics.
Chen
Shao-Peng
SP
Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P R China.
Wu
Jian
J
Yu
Xiao-Dong
XD
Xu
Jing-Juan
JJ
Chen
Hong-Yuan
HY
D000447:Aldehydes / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose; D002264:Carboxylic Acids / Q000097:blood; D003300:Copper; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D006046:Gold; D006801:Humans; D055432:Metabolomics / Q000295:instrumentation*; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D049329:Nanostructures
D000447:Aldehydes; D015415:Biomarkers; D001786:Blood Glucose; D002264:Carboxylic Acids; D004129:Dimethylpolysiloxanes; C013830:baysilon; D006046:Gold; D003300:Copper