title: Purification and characterization of HIV-human protein complexes.
abstract: To fully understand how pathogens infect their host and hijack key biological processes, systematic mapping of intra-pathogenic and pathogen-host protein-protein interactions (PPIs) is crucial. Due to the relatively small size of viral genomes (usually around 10-100 proteins), generation of comprehensive host-virus PPI maps using different experimental platforms, including affinity tag purification-mass spectrometry (AP-MS) and yeast two-hybrid (Y2H) approaches, can be achieved. Global maps such as these provide unbiased insight into the molecular mechanisms of viral entry, replication and assembly. However, to date, only two-hybrid methodology has been used in a systematic fashion to characterize viral-host protein-protein interactions, although a deluge of data exists in databases that manually curate from the literature individual host-pathogen PPIs. We will summarize this work and also describe an AP-MS platform that can be used to characterize viral-human protein complexes and discuss its application for the HIV genome.
authors: Department of Cellular and Molecular Pharmacology, University of California-San Francisco, 1700 4th Street, San Francisco, CA 94158, USA.
authors: Stefanie
authors: S
authors: Jäger
authors: Natali
authors: N
authors: Gulbahce
authors: Peter
authors: P
authors: Cimermancic
authors: Joshua
authors: J
authors: Kane
authors: Nanhai
authors: N
authors: He
authors: Seemay
authors: S
authors: Chou
authors: Iván
authors: I
authors: D'Orso
authors: Jason
authors: J
authors: Fernandes
authors: Gwendolyn
authors: G
authors: Jang
authors: Alan D
authors: AD
authors: Frankel
authors: Tom
authors: T
authors: Alber
authors: Qiang
authors: Q
authors: Zhou
authors: Nevan J
authors: NJ
authors: Krogan
mesh_terms: D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D016679:Genome, Viral; D015658:HIV Infections / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000378:metabolism*; D058605:Host-Derived Cellular Factors / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014162:Transfection
chemical_list: D058605:Host-Derived Cellular Factors; D054298:Human Immunodeficiency Virus Proteins; D011993:Recombinant Fusion Proteins