The expression patterns of a eukaryotic initiation factor 3 subunit H in the silk glands in Bombyx mori.
Eukaryotic initiation factor 3 subunit H has been characterized in many organisms, and it has been found to play many roles including help regulate translation initiation. In this work, we studied the tissue distribution and expression profiles of Bombyx mori (B. mori) eIF3 subunit H (BmeIF3h). BmeIF3h was prominently expressed in silk glands, with anterior silk glands (ASGs), middle silk glands (MSGs), and posterior silk glands (PSGs) all expressing BmeIF3h. The expression levels of BmeIF3h in MSGs and PSGs were higher than that in ASGs during 0 d and 2 d of the 5th instar larvae. The expression levels of BmeIF3h in MSGs and PSGs were up-regulated once the silk glands began to synthesize silk protein during the feeding stage of the 4th instar larvae. Immunohistochemistry showed that BmeIF3h was distributed in the cytoplasm of MSGs cells and in both the nucleus and the cytoplasm of PSGs cells. These data suggest that BmeIF3h had different action behaviors in the MSGs and PSGs related to the production of the silk glue proteins and silk fibre proteins, respectively.
Wang
Jia-Lin
JL
School of Life Sciences, Shandong University, Jinan 250100, Shandong, China.
Wang
Li-Fu
LF
Wang
Jin-Xing
JX
Zhao
Xiao-Fan
XF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D012831:Bombyx / Q000254:growth & development / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D039621:Eukaryotic Initiation Factor-3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000502:physiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D039621:Eukaryotic Initiation Factor-3; D007301:Insect Hormones