title: Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase.
abstract: Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How these events are coordinated with the cell cycle is poorly understood, particularly in mammalian cells. We report here, based on newly developed techniques for visualizing NPC formation, that cyclin-dependent kinases (Cdks), especially Cdk1 and Cdk2, promote interphase NPC formation in human dividing cells. Cdks seem to drive an early step of NPC formation because Cdk inhibition suppressed generation of 'nascent pores', which we argue are immature NPCs under the formation process. Consistent with this, Cdk inhibition disturbed proper expression and localization of some nucleoporins, including Elys/Mel-28, which triggers postmitotic NPC assembly. Strikingly, Cdk suppression did not notably affect nuclear growth, suggesting that interphase NPC formation and nuclear growth have distinct regulation mechanisms.
authors: Cellular Dynamics Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan. kmaeshim@lab.nig.ac.jp
authors: Kazuhiro
authors: K
authors: Maeshima
authors: Haruki
authors: H
authors: Iino
authors: Saera
authors: S
authors: Hihara
authors: Tomoko
authors: T
authors: Funakoshi
authors: Ai
authors: A
authors: Watanabe
authors: Masaomi
authors: M
authors: Nishimura
authors: Reiko
authors: R
authors: Nakatomi
authors: Kazuhide
authors: K
authors: Yahata
authors: Fumio
authors: F
authors: Imamoto
authors: Tsutomu
authors: T
authors: Hashikawa
authors: Hideo
authors: H
authors: Yokota
authors: Naoko
authors: N
authors: Imamoto
mesh_terms: D016203:CDC2 Protein Kinase / Q000378:metabolism*; D053145:Cell Nucleus Size; D020285:Cryoelectron Microscopy; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D020935:MAP Kinase Signaling System; D008855:Microscopy, Electron, Scanning; D022022:Nuclear Pore / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D016203:CDC2 Protein Kinase; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2