Alternative splicing of genes during neuronal differentiation of NT2 pluripotential human embryonal carcinoma cells.
We analyzed the mRNA diversity of genes after inducing neuronal differentiation in human NT2 teratocarcinoma cells using all-trans retinoic acid (RA). DNA microarray analyses of cells treated with RA identified 358 RA-responsive genes. mRNA diversity analysis revealed that 274 genes produced multiple protein-coding transcripts by alternative splicing. Among these 274 genes, we chose 26 genes that showed AS in their C-terminus and 12 transcription factor genes for further analysis. By using transcript-specific primers, we performed quantitative real-time PCR analysis to examine the expression profiles of all the protein-coding transcripts. Consequently, we identified genes which showed different RA-induced changes in the expression of their protein-coding transcripts.
Wakamatsu
Ai
A
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Imai
Jun-ichi
J
Watanabe
Shinya
S
Isogai
Takao
T
D017398:Alternative Splicing; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D055495:Neurogenesis / Q000187:drug effects / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014212:Tretinoin / Q000378:metabolism* / Q000494:pharmacology
D014157:Transcription Factors; D014212:Tretinoin