title: Synthesis and g-quadruplex-binding properties of defined acridine oligomers.
abstract: The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4-6.
authors: Institute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 15, 08028 Barcelona, Spain.
authors: Rubén
authors: R
authors: Ferreira
authors: Anna
authors: A
authors: Aviñó
authors: Ricardo
authors: R
authors: Pérez-Tomás
authors: Raimundo
authors: R
authors: Gargallo
authors: Ramon
authors: R
authors: Eritja