title: Protein phosphorylation in mitochondria --a study on fermentative and respiratory growth of Saccharomyces cerevisiae.
abstract: Phosphorylation as a posttranslational protein modification is a common subject of proteomic studies, but phosphorylation in mitochondria is still poorly investigated. The study presented here applied 2-DE to characterize phosphorylation in the yeast mitochondrial proteome and identified 59 spots corresponding to 34 phosphorylated mitochondrial or mitochondria-associated proteins. Most of these proteins presented putative substrates of mitogen-activated protein and target of rapamycin kinases, cAMP-dependent protein kinase, cyclin-dependent kinases and Snf1p suggesting them as key players in the phosphorylation of mitochondrial or mitochondria-associated proteins. The dynamic behaviour of the phosphoproteome under a major metabolic change, the shift from fermentation to respiration (diauxic shift), was further studied. Eight proteins (Ald4p, Eft1p/2p, Eno1p, Eno2p, Om14p, Pda1p, Qcr2p, Sdh1p) had growth dependent changes in their phosphorylation, indicating a role of phosphorylation-dependent regulation of translation, metabolic pathways (e.g. glucose fermentation, tricarboxylic acid cycle, pyruvate dehydrogenase and its bypass) and respiratory chain.
authors: Proteomics Core Facility, Biocenter Oulu, and Department of Biochemistry, University of Oulu, Oulu, Finland. Steffen.Ohlmeier@oulu.fi
authors: Steffen
authors: S
authors: Ohlmeier
authors: J Kalervo
authors: JK
authors: Hiltunen
authors: Ulrich
authors: U
authors: Bergmann
mesh_terms: D019069:Cell Respiration / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005285:Fermentation / Q000502:physiology*; D053858:Metabolic Networks and Pathways / Q000502:physiology; D008928:Mitochondria; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D024101:Mitochondrial Proteins; D010750:Phosphoproteins; D020543:Proteome