title: The glycosylated Gag protein of a murine leukemia virus inhibits the antiretroviral function of APOBEC3.
abstract: APOBEC proteins have evolved as innate defenses against retroviral infections. Human immunodeficiency virus (HIV) encodes the Vif protein to evade human APOBEC3G; however, mouse retroviruses do not encode a Vif homologue, and it has not been understood how they evade mouse APOBEC3. We report here a murine leukemia virus (MuLV) that utilizes its glycosylated Gag protein (gGag) to evade APOBEC3. gGag is critical for infection of in vitro cell lines in the presence of APOBEC3. Furthermore, a gGag-deficient virus restricted for replication in wild-type mice replicates efficiently in APOBEC3 knockout mice, implying a novel role of gGag in circumventing the action of APOBEC3 in vivo.
authors: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA.
authors: Angelo
authors: A
authors: Kolokithas
authors: Kyle
authors: K
authors: Rosenke
authors: Frank
authors: F
authors: Malik
authors: Duncan
authors: D
authors: Hendrick
authors: Lukas
authors: L
authors: Swanson
authors: Mario L
authors: ML
authors: Santiago
authors: John L
authors: JL
authors: Portis
authors: Kim J
authors: KJ
authors: Hasenkrug
authors: Leonard H
authors: LH
authors: Evans
mesh_terms: D000818:Animals; D003564:Cytidine Deaminase / Q000037:antagonists & inhibitors* / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D015683:Gene Products, gag / Q000737:chemistry / Q000502:physiology*; D006031:Glycosylation; D006801:Humans; D007113:Immunity, Innate; D009052:Leukemia Virus, Murine / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D007942:Leukemia, Experimental / Q000276:immunology / Q000821:virology; D051379:Mice; D018345:Mice, Knockout; D012192:Retroviridae Infections / Q000276:immunology / Q000821:virology; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology; D014779:Virus Replication / Q000276:immunology
chemical_list: D015683:Gene Products, gag; C503504:Apobec3 protein, mouse; D003564:Cytidine Deaminase