Influence of oxygen supply on liver condition and elimination of dimethylacetamide in the isolated perfused rat liver.
The effects were studied of improved oxygen supply on the integrity and metabolic activity towards dimethylacetamide of the isolated perfused rat liver. Improvement of oxygen supply by increased medium oxygenation or addition of chemical oxygen carriers (perfluortributylamine) or erythrocytes led to increased bile secretion. Leakage of lactate dehydrogenase and aspartate aminotransferase could be prevented during a 1-hr perfusion when either chemical oxygen carriers or erythrocytes were added. Improved medium oxygenation alone was not sufficient to prevent high enzyme leakage during the second half of the perfusion period. Histological evaluation confirmed the conclusion that less damage occurred when erythrocytes or perfluortributylamine were added to the perfusion medium. The metabolic clearance of dimethylacetamide by the perfused rat liver was not significantly improved when erythrocytes were added to the medium. The results show that addition of perfluortributylamine, or erythrocytes at a level of 4 g haemoglobin/litre, is necessary to maintain liver integrity for at least 1 hr in the liver perfusion system used in this study.
Palmen
N G
NG
University of Limburg, Department of Pharmacology, Section Toxicology, PO Box 616, 6200 MD Maastricht, The Netherlands.
Evelo
C T
CT
Borm
P J
PJ
Henderson
P T
PT