title: Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.
abstract: Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)-cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK-cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
authors: Department of Plant Systems Biology, VIB, Ghent, Belgium.
authors: Jelle
authors: J
authors: Van Leene
authors: Jens
authors: J
authors: Hollunder
authors: Dominique
authors: D
authors: Eeckhout
authors: Geert
authors: G
authors: Persiau
authors: Eveline
authors: E
authors: Van De Slijke
authors: Hilde
authors: H
authors: Stals
authors: Gert
authors: G
authors: Van Isterdael
authors: Aurine
authors: A
authors: Verkest
authors: Sandy
authors: S
authors: Neirynck
authors: Yelle
authors: Y
authors: Buffel
authors: Stefanie
authors: S
authors: De Bodt
authors: Steven
authors: S
authors: Maere
authors: Kris
authors: K
authors: Laukens
authors: Anne
authors: A
authors: Pharazyn
authors: Paulo C G
authors: PC
authors: Ferreira
authors: Nubia
authors: N
authors: Eloy
authors: Charlotte
authors: C
authors: Renne
authors: Christian
authors: C
authors: Meyer
authors: Jean-Denis
authors: JD
authors: Faure
authors: Jens
authors: J
authors: Steinbrenner
authors: Jim
authors: J
authors: Beynon
authors: John C
authors: JC
authors: Larkin
authors: Yves
authors: Y
authors: Van de Peer
authors: Pierre
authors: P
authors: Hilson
authors: Martin
authors: M
authors: Kuiper
authors: Lieven
authors: L
authors: De Veylder
authors: Harry
authors: H
authors: Van Onckelen
authors: Dirk
authors: D
authors: Inzé
authors: Erwin
authors: E
authors: Witters
authors: Geert
authors: G
authors: De Jaeger
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019295:Computational Biology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004261:DNA Replication; D008156:Luciferases / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015203:Reproducibility of Results
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D046912:Multiprotein Complexes; D008156:Luciferases; D018844:Cyclin-Dependent Kinases