Comet assay in neonatal sepsis.
OBJECTIVE
To determine whether the DNA damage detected using the Comet assay helps in the diagnosis of neonatal sepsis


METHODS
Sepsis was diagnosed, based on clinical findings, laboratory findings and positive culture results, in 24 of the 48 newborns who participated in the study. Hematological parameters, baseline demographic data and genotoxic evaluation were investigated.


RESULTS
There were no significant differences in hematological, demographic and genotoxic data between cases and controls.


CONCLUSIONS
The Comet assay proved to be ineffective in the diagnosis of neonatal sepsis.
Carvalho
Natália C
NC
Laboratório de Imunologia e Mutagênese, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
de Souza
Rozilda L
RL
Dal-Pizzol
Felipe
F
de Andrade
Vanessa Moraes
VM
D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D012372:ROC Curve; D018805:Sepsis / Q000175:diagnosis* / Q000235:genetics