[Analysis on pneumococcus isolated from part of china using serotype and pulsed-field gel electrophoresis].
OBJECTIVE
To investigate the serotype distribution and molecular features of Streptococcus pneumonia isolates in China.


METHODS
144 strains isolated from 6 provines were selected as the research object. The serotypes were determined with the method of capsular swelling. The molecular characteristics were conducted by optimized pulsed-field gel electrophoresis.


RESULTS
A total of 135 Streptococcus pneumoniae isolates were divided into 14 serogroups. 19, 6, 15, 23 serogroups are prevalent types in China. 9 isolates were failed to identify specific serogroups. All isolates could be divided into 92 pulsotypes. Pulsotypes were scattered. Absolutely dominant pulsotype was not found. Pulsotypes were similar among same serogroup isolates.


CONCLUSIONS
The pneumococcus of serogroup 19, 6, 15, 23 were common in China. PFGE had strong discriminatory ability of subtyping of Streptococcus pneumoniae. PFGE showed better epidemiological survey capacity.
Li
Ma-chao
MC
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhang
Qi
Q
Ren
Hong-yu
HY
D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D010802:Phylogeny; D011008:Pneumococcal Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral