Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: the effect of aerobic training and energy restriction.
The present study examined the effects of aerobic training and energy restriction on adipokines levels in mesenteric (MEAT) and retroperitoneal (RPAT) white adipose tissue from obese rats. Male Wistar rats were fed with standard laboratory diet (Control group) or high fat diet (HFD). After 15 weeks, HFD rats were randomly assigned to the following groups: rats submitted to HFD, which were sedentary (sedentary HFD, n=8) or trained (trained HFD, n=8); or submitted to energy-restriction (ER), which were sedentary (sedentary ER, n=8) or trained (trained ER, n=8). Trained rats ran on a treadmill at 55% VO(2max) for 60 min/day, 5 days/week, for 10 weeks. ER rats were submitted to a reduction of 20% daily caloric ingestion compared to the Control group. ER and aerobic training decreased body weight, MEAT and RPAT absolute weight, and fat mass. IL-6, IL-10 and TNF-α levels were decreased and adiponectin did not change in RPAT in response to ER protocol. On the other hand, ER and the aerobic training protocol decreased IL-6, TNF-α and adiponectin levels in MEAT. Absolute MEAT weight showed a positive correlation with IL-6 (r=0.464), TNF-α (r=0.508); and adiponectin (r=0.342). These results suggest a tissue-specific heterogeneous response in adipokines level. The combination of the protocols (aerobic training and energy restriction) did not induce an enhanced effect.
Yamashita
Alex Shimura
AS
Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Lira
Fábio Santos
FS
Rosa
José Cesar
JC
Paulino
Ellena Christina
EC
Brum
Patrícia Chakur
PC
Negrão
Carlos Eduardo
CE
dos Santos
Ronaldo Vagner
RV
Batista
Miguel Luiz
ML
do Nascimento
Claudia Oller
CO
Oyama
Lila Missae
LM
Seelaender
Marília
M
D054392:Adipokines / Q000378:metabolism*; D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002149:Energy Intake; D016014:Linear Models; D008297:Male; D009765:Obesity / Q000378:metabolism*; D010101:Oxygen Consumption; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
D054392:Adipokines; D052242:Adiponectin