Induction of heme oxygenase-1 by whisky congeners in human endothelial cells.
It is expected that the production of the cytoprotective heme oxygenase-1 (HO-1) protein in endothelial cells would reduce severity of vascular injuries, while phenolic compounds are known to induce HO-1 mRNA and protein in various cells. We investigated the activation of HO-1 by whisky, which contains various phenolic substances. The congeners of whisky stored from 4 to 18 y in oak barrels were shown to induce an increase of HO-1 protein in human umbilical vein endothelial cells, while those of freshly distilled whisky spirit exhibited no activity. To determine the compounds with potent HO-1-inducing activity among the whisky congeners, several chemicals that had been reported to exist in whisky or oak barrels were screened, and coniferyl aldehyde and sinapyl aldehyde showed the activity. Thus, compounds that emerged in whisky during barrel storage induced cytoprotective protein, HO-1, in human endothelial cells.
Suzuki
Keiko
K
Dept. of Radiation Emergency Medicine, Natl. Inst. of Radiological Sciences, Inage-ku, Chiba, Japan. rsc33591@nifty.com
Nemoto
Asuka
A
Tanaka
Izumi
I
Koshimizu
Seiichi
S
Suwa
Yoshihide
Y
Ishihara
Hiroshi
H
D000171:Acrolein / Q000031:analogs & derivatives; D000434:Alcoholic Beverages / Q000032:analysis*; D000447:Aldehydes / Q000494:pharmacology*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D005511:Food Handling; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D006801:Humans; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D013997:Time Factors; D014934:Wood
D000447:Aldehydes; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; C501624:sinapyl aldehyde; C075384:coniferaldehyde; D000171:Acrolein; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1