[Determination of five hormone drug residues in fish tissue by high performance liquid chromatography with gel permeation chromatographic clean-up].
A method was developed for the determination of five hormone residues in fish tissue by high performance liquid chromatography (HPLC) with gel permeation chromatographic (GPC) clean-up. The sample was extracted with ethyl acetate-methanol (8:2, v/v). The extract was cleaned-up on a Pharmadex LH-20 gel permeation column (450 mm x 15 mm) and eluted with methanol-ethyl acetate-acetic acid (800:200:2, v/v/v). The analysis was performed on an Agilent TC-C18 column (250 mm x 4. 6 mm, 5 microm) using acetonitrile-water (45:55, v/v) as the mobile phase at a flow rate of 1.2 mL/min and the detection wavelengths were set at 222 nm and 245 nm. All of the 5 hormone residues had good linear relationships (r > 0.999) in the range of 0.05-2.5 mg/L. The limits of detection (LOD) were from 10 to 24 microg/kg. The average recoveries for all the five hormone residues were from 60.1% to 89.0%, with relative standard deviations (RSDs) from 2.0% to 7.4%. The method is simple, rapid, and can be applied for the analysis of the five hormone residues in fish tissue.
Xie
Weiping
W
Department of Physical and Chemical Analysis, Quanzhou Center for Disease Control and Prevention, Quanzhou 362000, China. xweiping2000@163.com
Ouyang
Yanling
Y
Huang
Yingyu
Y
Chen
Chunzhu
C
D000818:Animals; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D004958:Estradiol / Q000032:analysis; D005396:Fish Products / Q000032:analysis*; D012739:Gonadal Steroid Hormones / Q000032:analysis*
D012739:Gonadal Steroid Hormones; D004958:Estradiol