Characterization and stability analysis of zinc oxide nanoencapsulated conjugated linoleic acid.
Nanoencapsulation technology has a diverse range of applications, including drug-delivery systems (DDS) and cosmetic and chemical carriers, because it can deliver various bio- and organic-molecules and improve their stabilities. Conjugated linoleic acid (CLA) has health benefits, including being an anticancer agent, but it decreases flavor due to volatiles from oxidation. To improve the stability of CLA for food applications, nanoencapsulated CLA was synthesized for use in zinc basic salt (ZBS) and characterized by powder X-ray diffractometry, thermogravimetric analysis (TGA), elemental CHN analysis, inductively coupled plasma (ICP) analysis, UV/VIS spectroscopy, and FTIR spectroscopy. The thermal stability of nanoencapsulated CLA at 180 degrees C, a temperature similar to that used in cooking, was analyzed by gas chromatography. The gallery height of nanoencapsulated CLA was determined to be approximately 26 A through powder X-ray diffractometry; therefore, the CLA molecules were closely packed with zig-zag form between the intracrystalline spaces of nano particles. Elemental CHN analysis and ICP data determined the chemical composition of nanoencapsulated CLA to be Zn(4.86)(OH)(8.78)(CLA)(0.94). By TGA, it was determined about 45% (wt/wt) of weight loss corresponded to CLA, which is good agreement with the 42.13% (wt/wt) determined from high-performance liquid chromatography (HPLC) and elemental CHN analysis. UV/VIS spectroscopy and Fourier-transformed infrared (FTIR) spectroscopy showed encapsulated CLA maintained a conjugated diene structure, supporting the presence of CLA. Nanoencapsulation improved the thermal stability of CLA by about 25%, compared to pristine CLA. Practical Application: This system can be used for protection of encapsulated negatively-charged food ingredients from thermal processing.
Choy
Jin-Ho
JH
Dept. of Chemistry, Ewha Womans Univ., Seoul, Korea.
Shin
Jiwon
J
Lim
Seung-Yong
SY
Oh
Jae-Min
JM
Oh
Mi-Hwa
MH
Oh
Sangsuk
S
D005524:Food Technology / Q000379:methods*; D005527:Food, Fortified; D006358:Hot Temperature / Q000009:adverse effects; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods; D009566:Nitrates / Q000737:chemistry; D010084:Oxidation-Reduction; D033362:Powder Diffraction; D013054:Spectrophotometry, Atomic; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D017967:Zinc Compounds / Q000737:chemistry; D015034:Zinc Oxide / Q000032:analysis / Q000737:chemistry*
D044243:Linoleic Acids, Conjugated; D009566:Nitrates; D017967:Zinc Compounds; C042103:zinc nitrate; D015034:Zinc Oxide