title: In vitro bioconversion of polyphenols from black tea and red wine/grape juice by human intestinal microbiota displays strong interindividual variability.
abstract: Dietary polyphenols in tea and wine have been associated with beneficial health effects. After ingestion, most polyphenols are metabolized by the colonic microbiota. The current study aimed at exploring the interindividual variation of gut microbial polyphenol bioconversion from 10 healthy human subjects. In vitro fecal batch fermentations simulating conditions in the distal colon were performed using polyphenols from black tea and a mixture of red wine and grape juice. Microbial bioconversion was monitored by NMR- and GC-MS-based profiling of diverse metabolites and phenolics. The complex polyphenol mixtures were degraded to a limited number of key metabolites. Each subject displayed a specific metabolite profile differing in composition and time courses as well as levels of these metabolites. Moreover, clear differences depending on the polyphenol sources were observed. In conclusion, varying metabolite pathways among individuals result in different metabolome profiles and therefore related health effects are hypothesized to differ between subjects.
authors: Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors: Gabriele
authors: G
authors: Gross
authors: Doris M
authors: DM
authors: Jacobs
authors: Sonja
authors: S
authors: Peters
authors: Sam
authors: S
authors: Possemiers
authors: John
authors: J
authors: van Duynhoven
authors: Elaine E
authors: EE
authors: Vaughan
authors: Tom
authors: T
authors: van de Wiele
mesh_terms: D000328:Adult; D001628:Beverages / Q000032:analysis*; D005243:Feces / Q000382:microbiology; D005260:Female; D005285:Fermentation; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008297:Male; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D059808:Polyphenols; D015203:Reproducibility of Results; D013662:Tea / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea