Intracerebroventricular injection of orexin-A stimulates monoamine metabolism but not HPA axis in neonatal chicks.
We investigated the effects of intracerebroventricular (ICV) injection of orexin-A on plasma corticosterone (CORT) concentration and brain monoamine metabolism to clarify the mechanism by which ICV orexin-A induced arousal in chicks. In Experiment 1, plasma CORT concentrations were measured as an indicator of hypothalamic-pituitary-adrenal (HPA) axis activity. There was no significant difference in CORT concentration between the control and orexin-A administered groups. In Experiment 2, the concentrations of monoamines (norepinephrine, dopamine and serotonin), their metabolites, and their metabolic turnover rates in the telencephalon, mesencephalon, and diencephalon were investigated. All metabolic turnover rates studied were increased at all brain sites after ICV orexin-A injection. In conclusion, the HPA axis does not appear to be involved in arousal-inducing mechanisms of orexin-A in neonatal chicks; however, several monoaminergic systems do.
Katayama
Sachiko
S
Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Shigemi
Kazutaka
K
Cline
Mark A
MA
Furuse
Mitsuhiro
M
D000818:Animals; D000831:Animals, Newborn; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D003345:Corticosterone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007276:Injections, Intraventricular / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D009479:Neuropeptides / Q000008:administration & dosage*; D018377:Neurotransmitter Agents / Q000008:administration & dosage*; D000068797:Orexins; D010913:Pituitary-Adrenal System / Q000187:drug effects*; D013997:Time Factors
D015306:Biogenic Monoamines; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D000068797:Orexins; D003345:Corticosterone