Molecular characterization, immunohistochemical localization and expression of a ribosomal protein L17 gene from Apis cerana cerana.
Ribosomal protein L17 (RPL17) is a core protein of the large (60S) ribosomal subunit and plays an important role in protein synthesis. In this report, a RPL17 gene was isolated from Apis cerana cerana, designated as AccRPL17. Alignment analysis showed that AccRPL17 exhibits high homology to other known RPL17s. Moreover, genomic sequence analysis revealed that five exons are splitted by four introns, and the position of the first intron is comparatively conservative, being localized in the 5' untranslated region. Partial putative cis-acting elements related to development were also examined. Quantitative real-time PCR showed that the highest mRNA level was detected in larvae on the fifth day. Simultaneously, immunohistochemical localization showed that AccRPL17 is primarily concentrated in muscular tissues, stigma, body wall, and the surrounding of the eye in the fifth-instar larvae. Further studies suggested that AccRPL17 might be involved in responses to abiotic stresses. This is a report attempting to analyze the expression and distribution of RPL17 in A. cerana cerana. These results indicated that AccRPL17 might play an important role in insect development, and the importance of AccRPL17 in participating in abiotic stresses is discussed.
Meng
Fei
F
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, People's Republic of China.
Zhang
Liang
L
Kang
Mingjiang
M
Guo
Xingqi
X
Xu
Baohua
B
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D040461:Gene Components; D007150:Immunohistochemistry; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D012269:Ribosomal Proteins; C032493:ribosomal protein L17