title: Development of a class-specific immunochromatographic strip test for the rapid detection of organophosphorus pesticides with a thiophosphate group.
abstract: In recent decades, organophosphorus (OP) pesticides are widely used in agricultural and domestic fields. There is increasing demand for more rapid and economical methods of detecting pesticide residues. We have developed a gold immunochromatography assay (GICA) strip test for detecting a group of OP pesticides based on monoclonal antibodies (MAb) against a generic hapten, O,O-diethyl O-(4-carboxy-3-methylphenyl) phosphorothioate. The MAb showed desirable properties for use in indirect competitive ELISA, and the minimum IC50 value (93 ng/mL) was observed in chlorpyrifos-ethyl curve. The partially purified monoclonal antibodies were conjugated with colloidal gold particles. The conjugation of the colloidal gold and antibodies was monitored by ultraviolet-visible (UV-vis) spectroscopy and fluorescence spectroscopy, while the transmission electron microscopy (TEM) images were used to characterize the particle size and shape of the conjugates. Dynamic light scattering (DLC) was applied as a less expensive and faster method to substitute TEM. The colloidal gold and antibody conjugates were used for the production of a GICA strip test to detect OP pesticides. Preliminary assessment suggests that the OP pesticide, chlorpyrifos-ethyl (CE), in environmental samples could be detected by the GICA strip. This assay could potentially be used for screening large volume of samples and on-spot monitoring before the precise analysis. But the homogeneity of the detection needs to be improved further.
authors: Faculty of Life Science, Northwestern Polytechnical University, Xian, Shanxi, P.R. China.
authors: Jing
authors: J
authors: Su
authors: Hui
authors: H
authors: Yang
authors: Jialei
authors: J
authors: Chen
authors: Huancai
authors: H
authors: Yin
authors: Ruihua
authors: R
authors: Tang
authors: Yuwei
authors: Y
authors: Xie
authors: Kai
authors: K
authors: Song
authors: Ting
authors: T
authors: Huyan
authors: Hua
authors: H
authors: Wang
authors: Wei
authors: W
authors: Wang
authors: Xiaoping
authors: X
authors: Xue
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004390:Chlorpyrifos; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017979:Gold Colloid / Q000737:chemistry*; D006241:Haptens / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009946:Organothiophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D010710:Phosphates / Q000737:chemistry*; D011934:Reagent Strips; D012015:Reference Standards
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D006241:Haptens; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; D010710:Phosphates; D011934:Reagent Strips; D004390:Chlorpyrifos; C035638:thiophosphoric acid