title: Gene expression enabling synthetic diversification of natural products: chemogenetic generation of pacidamycin analogs.
abstract: Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.
authors: School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK.
authors: Abhijeet
authors: A
authors: Deb Roy
authors: Sabine
authors: S
authors: Grüschow
authors: Nickiwe
authors: N
authors: Cairns
authors: Rebecca J M
authors: RJ
authors: Goss
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D001685:Biological Factors / Q000096:biosynthesis* / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015394:Molecular Structure; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001685:Biological Factors; D011741:Pyrimidine Nucleosides; D010088:Oxidoreductases; C407363:tryptophan halogenase