title: Enhancement of keratinocyte differentiation by rose absolute oil.
abstract: BACKGROUND
  Through differentiation processes, keratinocytes provide a physical barrier to our bodies and control skin features such as moisturization, wrinkles and pigmentation. Keratinocyte differentiation is disturbed in several skin diseases such as psoriasis and atopic dermatitis.
  
  
  OBJECTIVE
  The aim of this study is to evaluate the keratinocyte differentiation-enhancing effect of rose absolute oil (RAO).
  
  
  METHODS
  Primary cultured human normal keratinocytes were treated with RAO, and differentiation then checked by the expression of marker genes.
  
  
  RESULTS
  RAO did not induce cytotoxicity on cultured keratinocytes at a dose of 10microM. The level of involucrin, an early marker for keratinocyte differentiation, was significantly increased by RAO. Concomitantly, RAO increased involucrin promoter activity, indicating that RAO increased involucrin gene expression at the mRNA level. Furthermore, RAO increased the level of filaggrin in cultured keratinocytes, and in the granular layer of mouse skin. In line with these results, RAO decreased the proliferation of keratinocytes cultured in vitro. When RAO was applied topically on the tape-stripped mouse skins, it accelerated the recovery of disturbed barrier function.
  
  
  CONCLUSIONS
  These results suggest that RAO may be applicable for the control of skin texture and keratinocyte differentiation-related skin diseases.
authors: Department of Dermatology and Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju, Korea.
authors: Jin-Hwa
authors: JH
authors: Kim
authors: Dae-Kyoung
authors: DK
authors: Choi
authors: Sang-Sin
authors: SS
authors: Lee
authors: Sun Ja
authors: SJ
authors: Choi
authors: Chang Deok
authors: CD
authors: Kim
authors: Tae-Jin
authors: TJ
authors: Yoon
authors: Jeung-Hoon
authors: JH
authors: Lee