Calcium ion cyclotron resonance (ICR), 7.0 Hz, 9.2 microT magnetic field exposure initiates differentiation of pituitary corticotrope-derived AtT20 D16V cells.
The aim of this work is the study of the effect of electromagnetic radiations (ELF-EMF) tuned to the calcium cyclotron resonance condition of 7.0 Hz, 9.2 microT on the differentiation process of pituitary corticotrope-derived AtT20 D16V cells. These cells respond to nerve growth factor by extending neurite-like processes. To establish whether exposure to the field could influence the molecular biology of the pituitary gland, a corticotrope-derived cells line (AtT20 D16V) was exposed to ELF-EMF at a frequency of 7.0 Hz, 9.2 microT electromagnetic field by a Vega Select 719 power supply. Significant evidence was obtained to conclude that as little as 36 h exposure to the Ca(2+) ICR condition results in enhanced neurite outgrowth, with early expression and aggregation of the neuronal differentiation protein NF-200 into neurite structures.
Foletti
Alberto
A
BITitalia, Milan, Italy. contact@albertofoletti.ch
Ledda
Mario
M
De Carlo
Flavia
F
Grimaldi
Settimio
S
Lisi
Antonella
A
D002118:Calcium; D002454:Cell Differentiation / Q000528:radiation effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D052680:Corticotrophs / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D017357:Cyclotrons; D004574:Electromagnetic Fields; D016501:Neurites / Q000378:metabolism / Q000528:radiation effects; D016900:Neurofilament Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D013997:Time Factors
D016900:Neurofilament Proteins; C051774:neurofilament protein H; D002118:Calcium