Multiplex amplified nominal tandem-repeat analysis (MANTRA), a rapid method for genotyping Mycobacterium tuberculosis by use of multiplex PCR and a microfluidic laboratory chip.
A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.
Merritt
Adam J
AJ
Division of Microbiology & Infectious Diseases, PathWest Laboratory Medicine WA, Nedlands, WA, Australia.
Keehner
Terillee
T
O'Reilly
Lyn C
LC
McInnes
Russell L
RL
Inglis
Timothy J J
TJ
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology*