Regression of superficial glomerular podocyte injury in type 2 diabetic rats with overt albuminuria: effect of angiotensin II blockade.
OBJECTIVE
Clinical studies indicate that the remission, regression or both of nephrotic-range albuminuria are exerted by angiotensin II receptor blockers (ARBs) in diabetes. The current study was performed to test the hypothesis that these effects of ARBs are associated with regression of glomerular podocyte injury.


METHODS
We examined the effects of an ARB, olmesartan, on glomerular podocyte injury in type 2 diabetic Otsuka-Long-Evans-Tokushima-Fatty rats with overt albuminuria.


RESULTS
At baseline (55-week-old), diabetic Otsuka-Long-Evans-Tokushima-Fatty rats showed severe albuminuria with desmin-positive areas (an index of podocyte injury) in both superficial and juxtamedullary glomeruli, and podocyte injury was much greater in juxtamedullary than in superficial glomeruli. At 75-week-old, Otsuka-Long-Evans-Tokushima-Fatty rats had developed more severe albuminuria and superficial glomerular podocyte injury, whereas juxtamedullary glomerular podocyte injury did not advance further. Olmesartan (10 mg/kg per day) decreased albuminuria and superficial glomerular desmin staining to levels that were lower than those at baseline, whereas advanced juxtamedullary glomerular podocyte injury was not changed.


CONCLUSIONS
The current study demonstrates for the first time that juxtamedullary glomerular podocyte injury reaches a severe condition at an earlier time than superficial glomerular podocyte injury during the progression of overt albuminuria in type 2 diabetic rats. Our data also support the hypothesis that the antialbuminuric effects of ARBs are associated with regression of superficial glomerular podocyte injury in type 2 diabetes with nephrotic-range albuminuria.
Ihara
Genei
G
Cardiorenal and Cerebrovascular Internal Medicine, Kagawa University Medical School, Kagawa, Japan.
Kiyomoto
Hideyasu
H
Kobori
Hiroyuki
H
Nagai
Yukiko
Y
Ohashi
Naro
N
Hitomi
Hirofumi
H
Nakano
Daisuke
D
Pelisch
Nicolas
N
Hara
Taiga
T
Mori
Takefumi
T
Ito
Sadayoshi
S
Kohno
Masakazu
M
Nishiyama
Akira
A
D000419:Albuminuria / Q000473:pathology*; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000628:therapy*; D004195:Disease Models, Animal; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D050199:Podocytes / Q000473:pathology; D051381:Rats; D020318:Rats, Long-Evans; D012074:Remission Induction; D012598:Sclerosis / Q000473:pathology; D016896:Treatment Outcome
D000804:Angiotensin II