A fungicidal piperazine-1-carboxamidine induces mitochondrial fission-dependent apoptosis in yeast.
To unravel the working mechanism of the fungicidal piperazine-1-carboxamidine derivative BAR0329, we found that its intracellular accumulation in Saccharomyces cerevisiae is dependent on functional lipid rafts. Moreover, BAR0329 induced caspase-dependent apoptosis in yeast, in which the mitochondrial fission machinery consisting of Fis1 (Whi2), Dnm1 and Mdv1 is involved. Our data are consistent with a prosurvival function of Fis1 (Whi2) and a proapoptotic function of Dnm1 and Mdv1 during BAR0329-induced yeast cell death.
Bink
Anna
A
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.
Govaert
Gilmer
G
François
Isabelle E J A
IE
Pellens
Klaartje
K
Meerpoel
Lieven
L
Borgers
Marcel
M
Van Minnebruggen
Geert
G
Vroome
Valérie
V
Cammue
Bruno P A
BP
Thevissen
Karin
K
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000935:Antifungal Agents / Q000633:toxicity*; D017209:Apoptosis; D020558:GTP Phosphohydrolases / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects*; D024101:Mitochondrial Proteins / Q000378:metabolism; D010879:Piperazines / Q000633:toxicity*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000935:Antifungal Agents; C464859:FIS1 protein, S cerevisiae; C429283:MDV1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D010879:Piperazines; D029701:Saccharomyces cerevisiae Proteins; D020558:GTP Phosphohydrolases; C094774:DNM1 protein, S cerevisiae