Axon fasciculation in the developing olfactory nerve.
Olfactory sensory neuron (OSN) axons exit the olfactory epithelium (OE) and extend toward the olfactory bulb (OB) where they coalesce into glomeruli. Each OSN expresses only 1 of approximately 1,200 odor receptors (ORs). OSNs expressing the same OR are distributed in restricted zones of the OE. However, within a zone, the OSNs expressing a specific OR are not contiguous - distribution appears stochastic. Upon reaching the OB the OSN axons expressing the same OR reproducibly coalesce into two to three glomeruli. While ORs appear necessary for appropriate convergence of axons, a variety of adhesion associated molecules and activity-dependent mechanisms are also implicated. Recent data suggest pre-target OSN axon sorting may influence glomerular convergence. Here, using regional and OR-specific markers, we addressed the spatio-temporal properties associated with the onset of homotypic fasciculation in embryonic mice and assessed the degree to which subpopulations of axons remain segregated as they extend toward the nascent OB. We show that immediately upon crossing the basal lamina, axons uniformly turn sharply, usually at an approximately 90° angle toward the OB. Molecularly defined subpopulations of axons show evidence of spatial segregation within the nascent nerve by embryonic day 12, within 48 hours of the first OSN axons crossing the basal lamina, but at least 72 hours before synapse formation in the developing OB. Homotypic fasciculation of OSN axons expressing the same OR appears to be a hierarchical process. While regional segregation occurs in the mesenchyme, the final convergence of OR-specific subpopulations does not occur until the axons reach the inner nerve layer of the OB.
Miller
Alexandra M
AM
Department of Neurosurgery, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.
Maurer
Lydia R
LR
Zou
Dong-Jing
DJ
Firestein
Stuart
S
Greer
Charles A
CA
D000367:Age Factors; D000818:Animals; D001369:Axons / Q000502:physiology*; D053148:Caspase 3 / Q000172:deficiency; D004622:Embryo, Mammalian; D005207:Fasciculation / Q000378:metabolism / Q000473:pathology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods; D008648:Mesoderm / Q000166:cytology / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D004676:Myelin Basic Protein / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D039942:Neuropilin-1; D009589:Nitrogen Oxides / Q000378:metabolism; D009832:Olfactory Nerve / Q000166:cytology* / Q000196:embryology*; D018034:Olfactory Receptor Neurons / Q000166:cytology*; D011247:Pregnancy; D018035:Receptors, Odorant / Q000235:genetics
D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D009589:Nitrogen Oxides; D018035:Receptors, Odorant; D039942:Neuropilin-1; D049452:Green Fluorescent Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse; D053148:Caspase 3