Comparative transcriptional profiling and preliminary study on heterosis mechanism of super-hybrid rice.
Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F(1) super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F(1) hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F(1) hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.
Song
Gui-Sheng
GS
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Zhai
Hong-Li
HL
Peng
Yong-Gang
YG
Zhang
Lei
L
Wei
Gang
G
Chen
Xiao-Ying
XY
Xiao
Yu-Guo
YG
Wang
Lili
L
Chen
Yue-Jun
YJ
Wu
Bin
B
Chen
Bin
B
Zhang
Yu
Y
Chen
Hua
H
Feng
Xiu-Jing
XJ
Gong
Wan-Kui
WK
Liu
Yao
Y
Yin
Zhi-Jie
ZJ
Wang
Feng
F
Liu
Guo-Zhen
GZ
Xu
Hong-Lin
HL
Wei
Xiao-Li
XL
Zhao
Xiao-Ling
XL
Ouwerkerk
Pieter B F
PB
Hankemeier
Thomas
T
Reijmers
Theo
T
van der Heijden
Rob
R
Lu
Cong-Ming
CM
Wang
Mei
M
van der Greef
Jan
J
Zhu
Zhen
Z
D057486:Carbon Cycle / Q000235:genetics; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks / Q000235:genetics; D006823:Hybrid Vigor / Q000235:genetics*; D006824:Hybridization, Genetic / Q000235:genetics*; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010788:Photosynthesis / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*