[Establishment nephrolithiasis rat model induced by nanobacteria and analysis of stone formation].
OBJECTIVE
To establish nephrolithiasis rat model induced by nanobacteria and to determine the relationship of nanobacteria with nephrolithiasis.


METHODS
Thirty adult male SD rats were randomized into 3 groups (n=10 each): group A, given an intravenous injection of nanobacteria; group B, given an intravenous injection of nanobacteria and taken tetracycline; group C, as normal control. Eight weeks later, all rats were sacrificed and the kidneys were examined for pathology and the 24-h urine N-acetyl-beta-D-aminoglycoside enzyme (NAG) and lactate dehydrogenase (LDH) were detected.


RESULTS
Histopathological studies revealed nanobacteria induced renal tubular crystallization, which was significantly different among the 3 groups (P=0.033). The crystallization was higher in group A than that in group B. The urinary NAG and LDH excretion were significantly different among the 3 groups (P<0.001).


CONCLUSIONS
Nanobacteria may be involved in the pathogenesis of nephrolithiasis. Tetracycline may inhibit stone formation.
Hu
Wei-guo
WG
Department of Urology, Peking University People's Hospital, Beijing 100034, China.
Wang
Xiao-feng
XF
Xu
Tao
T
Li
Jian-xing
JX
Chen
Liang
L
Yu
Cheng-fan
CF
Huang
Xiao-bo
XB
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004195:Disease Models, Animal; D007669:Kidney Calculi / Q000382:microbiology*; D008297:Male; D053758:Nanoparticles; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley