title: [Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis].
abstract: The nucleotide sequences of the inv, yadA, and ail adhesin-invasin genes were analyzed in 24 strains of the main and nonmain Yersinia pestis subspecies, which were isolated from natural plague foci in Russia and neighbor countries, and ten Y. pseudotuberculosis strains. All of the five plague agent subspecies (main, caucasica, altaica, ulegeica, and hissarica) had the inv and yadA genes altered by insertion of the IS element and a single nucleotide deletion, respectively, as was earlier observed for the Y. pestis strains KIM and CO92. Consequently, the strains lacked functional activity of the Inv and YadA proteins. The ail gene of the main and ulegeica subspecies had a missense mutation, which replaced Val138 with Phe in the Ail protein. The strains of the caucasica subspecies had an AGT insertion in the ail gene, resulting in Ser148 insertion in the polypeptide chain. The changes in the ail sequence probably exerted no effect on ail expression, since the strains of all subspecies were resistant to blood serum complement.
authors: G A
authors: GA
authors: Eroshenko
authors: G N
authors: GN
authors: Odinokov
authors: L M
authors: LM
authors: Kukleva
authors: Ia M
authors: IaM
authors: Krasnov
authors: V V
authors: VV
authors: Kutyrev
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001770:Blood Bactericidal Activity; D004251:DNA Transposable Elements / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D016254:Mutagenesis, Insertional; D011817:Rabbits; D012426:Russia; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; C526543:Ail protein, Yersinia pestis; D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D037521:Virulence Factors; C064832:YadA protein, Yersinia; C053476:invasin, Yersinia