Uptake of macromolecules by cercariae during skin penetration and transformation to schistosomula (Schistosoma mansoni).
Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and then diffuse throughout the body of the parasite. We present a hypothesis that the internal cells of the body of the schistosomulum represent a new host-parasite interface, at which skin-derived growth factors may stimulate receptors on internal membranes during transformation of the cercariae into the schistosomulum.
Thornhill
Joyce
J
University of Glasgow, Scotland, UK.
Kusel
John
J
Oliviera
Fabricia Alvisi de
FA
Ribeiro
Fabio
F
Lima
Symone Fulgêncio
SF
Coelho
Paulo Marcos Zech
PM
McVeigh
Paul
P
Mattos
Ana Carolina Alves
AC
D000818:Animals; D005456:Fluorescent Dyes / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000502:physiology*; D007546:Isoquinolines / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D011419:Propidium / Q000378:metabolism; D012550:Schistosoma mansoni / Q000254:growth & development*; D012867:Skin / Q000469:parasitology*
D005456:Fluorescent Dyes; D007546:Isoquinolines; D011419:Propidium; C017475:lucifer yellow