title: Culture and differentiation of osteoblasts on coral scaffold from human bone marrow mesenchymal stem cells.
abstract: In this paper we describe an approach that aims to provide fundamental information towards a scientific, biomechanical basis for the use of natural coral scaffolds to initiate mesenchymal stem cells into osteogenic differentiation for transplant purposes. Biomaterial, such as corals, is an osteoconductive material that can be used to home human derived stem cells for clinical regenerative purposes. In bone transplantation, the use of biomaterials may be a solution to bypass two main critical obstacles, the shortage of donor sites for autografts and the risk of rejection with allograft procedures. Bone regeneration is often needed for multiple clinical purposes for instance, in aesthetic reconstruction and regenerative procedures. Coral graft Porites lutea has been used by our team for a decade in clinical applications on over a thousand patients with different bone pathologies including spinal stenosis and mandibular reconstruction. It is well accepted that human bone marrow (hBM) is an exceptional source of mesenchymal stem cells (MSCs), which may differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. Isolated MSCs from human bone marrow were induced into osteoblasts using an osteogenic medium enriched with two specific growth factors, FGF9 and vitamin D2. Part of the cultured MSCs were directly transferred and seeded onto coral scaffolds (Porites Lutea) and induced to differentiate into osteoblasts and part were cultured in flasks for osteocell culture. The data support the concept that hBM is a reliable source of MSCs which may be easily differentiated into osteoblasts and seeded into coral as an optimal device for clinical application. Within this project we have also discussed the biological nature of MSCs, their potential application for clinical transplantation and the prospect of their use in gene therapy.
authors: Department of Histology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach University of Medicine, Ho Chi Minh city, Vietnam. toaiphd@yahoo.com
authors: Cong Toai
authors: CT
authors: Tran
authors: Ciro
authors: C
authors: Gargiulo
authors: Huynh Duy
authors: HD
authors: Thao
authors: Huynh Minh
authors: HM
authors: Tuan
authors: Luis
authors: L
authors: Filgueira
authors: D
authors: D
authors: Michael Strong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology*; D001861:Bone Regeneration / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult
chemical_list: D003470:Culture Media