Rapid and sensitive determination of nicotine in formulations and biological fluid using micellar liquid chromatography with electrochemical detection.
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH(2)PO(4) 0.01 M (pH 6)-KCl 0.001 M, with electrochemical detection at 0.8 V. In the optimization step, the influence of the modifiers propanol, butanol and pentanol, and the voltage has been studied. With the proposed method the analysis time is below than 8 min, linearity better than 0.999, limits of detection and quantification (ng/ml) was 4 and 12 respectively, repeatability and intermediate precision below 1.8%, and all these parameters are adequate for the quantification of nicotine in chewing gum, dermal patches, tobacco and serum samples either by a pharmacologist, pathologist or toxicologist.
Chin-Chen
Mei-Liang
ML
Area de Química Analítica, Universitat Jaume I, Campus Riu Sec, 12071 Castelló, Spain.
Rambla-Alegre
Maria
M
Durgavanshi
Abhilasha
A
Bose
Devasish
D
Esteve-Romero
Josep
J
D002638:Chewing Gum / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016018:Least-Squares Analysis; D009538:Nicotine / Q000032:analysis* / Q000097:blood; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate; D020340:Tobacco Use Cessation; D057968:Transdermal Patch
D002638:Chewing Gum; D012967:Sodium Dodecyl Sulfate; D009538:Nicotine