Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides.
Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) [P(IPAAm-co-tBAAm)] brushes were prepared on poly(hydroxy methacrylate) (PHMA) [hydrolyzed poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)] beads having large pores by surface-initiated atom transfer radical polymerization (ATRP) and applied to the stationary phases of thermo-responsive chromatography. Optimized amount of copolymer brushes grafted PHMA beads were able to separate peptides and proteins with narrow peaks and a high resolution. The beads were found to have a specific surface area of 43.0 m(2)/g by nitrogen gas adsorption method. Copolymer brush of P(IPAAm-co-tBAAm) grafted PHMA beads improved the stationary phase of thermo-responsive chromatography for the all-aqueous separation of peptides and proteins.
Mizutani
Aya
A
Graduate School of Pharmaceutical Sciences, Keio University, 1-5-14 Shibakoen, Minato, Tokyo 105-0011, Japan.
Nagase
Kenichi
K
Kikuchi
Akihiko
A
Kanazawa
Hideko
H
Akiyama
Yoshikatsu
Y
Kobayashi
Jun
J
Annaka
Masahiko
M
Okano
Teruo
T
D000178:Acrylamides / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007328:Insulin / Q000302:isolation & purification; D008863:Microspheres; D008970:Molecular Weight; D009584:Nitrogen; D010446:Peptide Fragments / Q000302:isolation & purification*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013696:Temperature
D000178:Acrylamides; D007328:Insulin; D010446:Peptide Fragments; D011109:Polymethacrylic Acids; C079805:poly(N-isopropylacrylamide-N-tert-butylacrylamide)copolymer; D009584:Nitrogen