LRRK2 is expressed in B-2 but not in B-1 B cells, and downregulated by cellular activation.
LRRK2, the causal molecule of familial Parkinson's disease, is expressed strongly by one of the B cell subsets, B-2 cells, but not by the other subset, B-1 cells, in the mouse peritoneal cavity, spleen, and peripheral blood. Bone marrow pre-B cells or T cells exhibited little LRRK2 expression. LRRK2 expression was dramatically downregulated upon activation of B-2 cells with various types of stimulation. These results suggest that LRRK2, whose true function has not yet been clarified, may play some important role(s) in the development and function of B cells, particularly the maintenance of B-2 cells in a resting status.
Kubo
Makoto
M
Division of Clinical Immunology, Graduate School of Medical Sciences, Kitasato University, Minami-ku, Sagamihara, Kanagawa, Japan.
Kamiya
Yoshiko
Y
Nagashima
Ryuichi
R
Maekawa
Tatsunori
T
Eshima
Koji
K
Azuma
Sadahiro
S
Ohta
Etsuro
E
Obata
Fumiya
F
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D000097782:NF-kappaB-Inducing Kinase
D015759:Ionomycin; D007476:Ionophores; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D008070:Lipopolysaccharides; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate; D000097782:NF-kappaB-Inducing Kinase; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; C495279:Lrrk2 protein, mouse