Fluorouracil selectively enriches stem-like leukemic cells in a leukemic cell line.
Recent studies have reported that cancer stem cells (CSCs) could be isolated from solid cancer cell lines, in which the purity of CSCs was higher than that from tumor tissues. Separation of CSCs from leukemic cell lines was rarely reported. In this study, CD34(+)CD38(-)stem-like cell subsets in human KG-1a leukemic cell line were enriched by cytotoxic agent 5-fluorouracil (5-FU). After 4 days incubation of KG-1a cell line with 5-FU (50 microg/ml), the CD34(+)CD38(-) subpopulation of cell lines was enriched more than 10 times. The enriched cells had proliferate potential in vitro, low level of RNA transcription and Hoechst 33342 dye efflux ability, accompanied by high expression of ATP-binding cassette transporter protein ABCG2. Our findings suggest that treatment with 5-FU offers an easy method to isolate leukemic stem-like subpopulation. It can facilitate studies of leukemic stem cell biology and the development of new therapeutic strategies.
Zhang
Ling
L
1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Key Laboratory, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. cqumszhl@sina.com
Yang
Song
S
He
Yu-Juan
YJ
Shao
Hui-Yuan
HY
Wang
Li
L
Chen
Hui
H
Gao
Yu-Jie
YJ
Qing
Feng-Xian
FX
Chen
Xian-Chun
XC
Zhao
Liu-Yang
LY
Tan
Shi
S
D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001562:Benzimidazoles / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D012313:RNA / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; D018952:Antigens, CD34; D001562:Benzimidazoles; D009363:Neoplasm Proteins; D012313:RNA; D004247:DNA; D051997:ADP-ribosyl Cyclase 1; C017807:bisbenzimide ethoxide trihydrochloride; D005472:Fluorouracil