title: [Bioinformatics analysis of mosquito densovirus nostructure protein NS1].
abstract: OBJECTIVE
  To analyze and predict the structure and function of mosquito densovirus (MDV) nostructual protein1 (NS1).
  
  
  METHODS
  Using different bioinformatics software, the EXPASY pmtparam tool, ClustalX1.83, Bioedit, MEGA3.1, ScanProsite, and Motifscan, respectively to comparatively analyze and predict the physic-chemical parameters, homology, evolutionary relation, secondary structure and main functional motifs of NS1.
  
  
  RESULTS
  MDV NS1 protein was a unstable hydrophilic protein and the amino acid sequence was highly conserved which had a relatively closer evolutionary distance with infectious hypodermal and hematopoietic necrosis virus (IHHNV). MDV NS1 has a specific domain of superfamily 3 helicase of small DNA viruses. This domain contains the NTP-binding region with a metal ion-dependent ATPase activity. A virus replication roller rolling-circle replication(RCR) initiation domain was found near the N terminal of this protein. This protien has the biological function of single stranded incision enzyme.
  
  
  CONCLUSIONS
  The bioinformatics prediction results suggest that MDV NS1 protein plays a key role in viral replication, packaging, and the other stages of viral life.
authors: Institute of Genetic Engineering, Guangzhou 510515, China.
authors: Yun-qiao
authors: YQ
authors: Dong
authors: Wen-li
authors: WL
authors: Ma
authors: Jin-bao
authors: JB
authors: Gu
authors: Wen-ling
authors: WL
authors: Zheng
mesh_terms: D000818:Animals; D019295:Computational Biology; D009033:Culicidae / Q000821:virology*; D017990:Densovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins