Static magnetic fields enhanced the potency of cisplatin on k562 cells.
OBJECTIVE
This study investigates whether 8.8 mT static magnetic fields (SMFs) can enhance the killing potency of cisplatin (DDP) on human leukemic cells (K562).


METHODS
The cell proliferation, cell cycle distribution, DNA damage, and the change in cell surface ultrastructure after K562 cells were exposed to 8.8 mT SMFs with or without DDP were analyzed.


RESULTS
The results show that SMFs enhanced the killing effect of DDP on K562 cells, reducing the efficient killing concentration of DDP on K562 cells from 20 to 10 microg/mL. Atomic force microscope observation showed that the cell surface ultrastructure was altered. The results of fluorescence-activated cell sorting analysis indicated that K562 cells treated with SMF plus DDP were arrested at the S phase. The SMF exposure induced DNA to become thicker than controls, and breakage of DNA occurred in the DDP group; however, DNA breakage was increased in the SMF + DDP group.


CONCLUSIONS
The results show that SMFs enhanced the anticancer effect of DDP on K562 cells. The mechanism correlated with the DNA damage model. This study also shows the potentiality of SMFs as an adjunctive treatment method for chemotherapy.
Chen
Wen-Fang
WF
Shaanxi Normal University, Xi'an, China.
Qi
Hao
H
Sun
Run-Guang
RG
Liu
Ying
Y
Zhang
Kun
K
Liu
Jian-Qiang
JQ
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004357:Drug Synergism; D004361:Drug Tolerance / Q000528:radiation effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D055909:Magnetic Field Therapy; D018625:Microscopy, Atomic Force
D000970:Antineoplastic Agents; D002945:Cisplatin