title: IL-17 contributes to autoimmune hepatitis.
abstract: The role of interleukin-17 (IL-17) in autoimmune hepatitis (AIH) was investigated. A mouse model of experimental autoimmune hepatitis was established, and the syngeneic S-100 antigen emulsified in complete Freud's adjuvant was injected intraperitoneally into adult male C57BL/6 mice. The IL-17 expression in serum and the livers of the mice models was detected by using ELISA and immunohistochemistry, respectively. IL-17 neutralizing antibody was used to study the biological effect of IL-17 in the experimental AIH. IL-17 neutralizing antibody in vivo administration alleviated the hepatic inflammation and ALT level in the AIH model. IL-17 in the peripheral blood mononuclear cells (PBMC) of AIH patients was measured by using real-time PCR method. The results showed that IL-17 level was significantly up-regulated in AIH patients and mice models. It was concluded that IL-17 contributed to the development of AIH and might be a potential therapeutic target of AIH.
authors: Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. yhjmarry@hotmail.com
authors: Haijing
authors: H
authors: Yu
authors: Jiaquan
authors: J
authors: Huang
authors: Yang
authors: Y
authors: Liu
authors: Guo
authors: G
authors: Ai
authors: Weiming
authors: W
authors: Yan
authors: Xiaojing
authors: X
authors: Wang
authors: Qin
authors: Q
authors: Ning
mesh_terms: D000818:Animals; D005260:Female; D005620:Freund's Adjuvant; D019693:Hepatitis, Autoimmune / Q000209:etiology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D020381:Interleukin-17 / Q000097:blood* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009418:S100 Proteins; D015854:Up-Regulation
chemical_list: D020381:Interleukin-17; D009418:S100 Proteins; D005620:Freund's Adjuvant