title: [Detection of molecular epidemiology of Mycobacterium gordonae isolates].
abstract: OBJECTIVE
  To analyze the molecular epidemiology of Mycobacterium gordonae strains from patients and environments in the hospital.
  
  
  METHODS
  A total of 46 clinical strains were obtained from patients registered at the NHO Kinki-chuo Chest Medical Center and 3 strains from hospital environments.
  
  
  METHODS
  By using genetic data from the 16S rRNA gene and hsp65PRA, pulsed-field gel electrophoresis (PFGE) assessment of their intraspecies variability and epidemiology was carried out.
  
  
  RESULTS
  Strains from six patients and environmental cultures exhibited the different genotypes of 16S rRNA gene sequencing and the hsp65PRA type. The PFGE analysis suggested no pseudo-outbreak and showed a polyclonal infection in one patient.
  
  
  CONCLUSIONS
  These findings suggest that we should maintain effective surveillance of environments in the hospital and continuously perform molecular epidemiological investigations for infection control of M. gordonae.
authors: Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan. dustin@kch.hosp.go.jp
authors: Shiomi
authors: S
authors: Yoshida
authors: Katsuhiro
authors: K
authors: Suzuki
authors: Tomotada
authors: T
authors: Iwamoto
authors: Kazunari
authors: K
authors: Tsuyuguchi
authors: Motohisa
authors: M
authors: Tomita
authors: Masaji
authors: M
authors: Okada
authors: Mitsunori
authors: M
authors: Sakatani
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000032:analysis; D018834:Chaperonin 60 / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology*; D009170:Nontuberculous Mycobacteria / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium