Mutation of charged residues to neutral ones accelerates urea denaturation of HP-35.
Following the studies of urea denaturation of β-hairpins using molecular dynamics, in this paper, molecular dynamics simulations of two peptides, a 35 residue three helix bundle villin headpiece protein HP-35 and its doubly norleucine-substituent mutant (Lys24Nle/Lys29Nle) HP-35 NleNle, were undertaken in urea solutions to understand the molecular mechanism of urea denaturation of α-helices. The mutant HP-35 NleNle was found to denature more easily than the wild type. During the expansion of the small hydrophobic core, water penetration occurs first, followed by that of urea molecules. It was also found that the initial hydration of the peptide backbone is achieved through water hydrogen bonding with the backbone CO groups during the denaturation of both polypeptides. The mutation of the two charged lysine residues to apolar norleucine enhances the accumulation of urea near the hydrophobic core and facilitates the denaturation process. Urea also interacts directly with the peptide backbone as well as side chains, thereby stabilizing nonnative conformations. The mechanism revealed here is consistent with the previous study on secondary structure of β-hairpin polypeptide, GB1, PEPTIDE 1, and TRPZIP4, suggesting that there is a general mechanism in the denaturation of protein backbone hydrogen bonds by urea.
Wei
Haiyan
H
Department of Chemistry, Texas A&M University, P.O. Box 3012, College Station, Texas 77842, USA.
Yang
Lijiang
L
Gao
Yi Qin
YQ
D000818:Animals; D002645:Chickens; D006860:Hydrogen Bonding; D008239:Lysine / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D016900:Neurofilament Proteins / Q000737:chemistry / Q000235:genetics; D009646:Norleucine / Q000737:chemistry / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D010649:Phenylalanine / Q000737:chemistry; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D014508:Urea / Q000737:chemistry
D016900:Neurofilament Proteins; D010446:Peptide Fragments; C115223:villin headpiece subdomain peptide; D010649:Phenylalanine; D009646:Norleucine; D014508:Urea; D008239:Lysine