Determination of fatty acids in human sweat during fasting using GC/MS.
Fatty acids (FAs) are biological molecules that are used as major metabolic fuels, and are concerned in important metabolic processes. We have performed a non-invasive and technically rapid and simple method for collecting sweat from humans, followed by GC/MS determination. The sweat was collected from each volunteer (the middle finger) by spraying 70% ethanol aqueous solution (no harmful solvent) into a 1.5-cm(3) plastic vial. Analysis of FAs in sweat showed that the sweat solution contains lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), oleic acid (C18:1), and stearic acid (C18:0). Here, it is demonstrated that FA concentrations for 4 young subjects correlated positively with percent of body fat (r = 0.78) and that the total FA levels for them increased progressively with increasing fasting time when a subject fasted throughout the experiment.
Nunome
Yoko
Y
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan. nunome.yoko@b.mbox.nagoya-u.ac.jp
Tsuda
Takao
T
Kitagawa
Kuniyuki
K
D000273:Adipose Tissue; D000328:Adult; D005215:Fasting; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D013542:Sweat / Q000378:metabolism*; D013997:Time Factors
D005227:Fatty Acids