Pentacyclic triterpenoids from olive fruit and leaf.
This work establishes a new procedure for the extraction and analysis of pentacyclic triterpenes, with which fruits and leaves from three Spanish olive cultivars ("Picual", "Hojiblanca", and "Arbequina") has been studied. The leaf contains important amounts of oleanolic acid (3.0-3.5% DW), followed by significant concentrations of maslinic acid and minor levels of ursolic acid, erythrodiol, and uvaol. The abundance and profile of triterpenoids change during the leaf ontogeny. In the fruit, triterpenes are exclusively located in the epicarp at concentrations 30-fold lower than that in the leaf. Maslinic acid is the main triterpenoid, only accompanied of oleanolic acid. Along the ripening the levels of these triterpenes decreased. All the analyzed leaves and fruits come from the same agricultural estate, with identical climate and culturing conditions. For this reason, the found differences could majorly be attributable to the genetic factors of the olive cultivars.
Guinda
Angeles
A
Instituto de la Grasa-CSIC, Av. Padre Garcia Tejero, 4, 41012 Seville, Spain.
Rada
Mirela
M
Delgado
Teresa
T
Gutiérrez-Adánez
Pilar
P
Castellano
José María
JM
D002849:Chromatography, Gas; D031658:Olea / Q000737:chemistry*; D012015:Reference Standards; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
D014315:Triterpenes