Structure of the O-polysaccharide of Cronobacter sakazakii O1 containing 3-(N-acetyl-l-alanyl)amino-3,6-dideoxy-d-glucose.
The O-polysaccharide (O-antigen) was released by mild acid hydrolysis of the lipopolysaccharide of Cronobacter sakazakii ATCC 29544(T) (serotype O1) and studied by composition analysis and Smith degradation, in addition to 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: [Formula: see text] where d-Qui3NAcyl stands for 3-(N-acetyl-l-alanyl)amino-3,6-dideoxy-d-glucose. The same composition but a different structure has been reported earlier for the O-polysaccharide of C. sakazakii 3290 [MacLean, L. L.; Pagotto, F.; Farber, J. M.; Perry, M. B. Biochem. Cell Biol.2009, 87, 459-465].
Arbatsky
Nikolay P
NP
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Wang
Min
M
Shashkov
Alexander S
AS
Feng
Lu
L
Knirel
Yuriy A
YA
Wang
Lei
L
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D002240:Carbohydrate Sequence; D044083:Cronobacter sakazakii / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D000117:Acetylglucosamine