[Analyze the bioactivity of PD-1 and PD-L1 recombination protein which expressed by prokaryotic system].
OBJECTIVE
To analyze the interaction of PD-1 and PD-L1 recombination protein and to know their bioactivity and affinity.


METHODS
Stick the PD-1 protein on the surface of CM5 sensor chip by the method of Ammine coupling after being preconsentrated. Dilute the PD-L1 protein step by step and reject it to the passage on CM5 sensor chip which had been stick by PD-1. The time of combination is 3 minutes and of separation is 15 minutes, respectively. Observe the procession and analyze data by BIA Evaluation software 4.


RESULTS
On the consistency of 40 microg/ml, pH 4.5, the PD-1 protein could couple steady on the surface of CM5sensor chip, RU is 3300. On the density of 200 mmol/ml PD-L1 could combine with PD-1 specifically, RU = 150, K(D) = 3.5 x 10(-6).


CONCLUSIONS
The PD-1 and PD-L1 recombination protein which we expressed by prokaryotic system have good affinity and bioactivity. The results could provide basic condition for later study.
Shen
Li-ping
LP
National Institute for Disease Control and Prevention, China CDC, Beijing 100052, China. slpss128@126.com
Xu
Long
L
Li
Jian-dong
JD
Chen
Si-yong
SY
Guo
Yu
Y
Qiu
Feng
F
Bi
Sheng-li
SL
D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D060890:B7-H1 Antigen; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D061026:Programmed Cell Death 1 Receptor; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D015703:Antigens, CD; D051017:Apoptosis Regulatory Proteins; D060890:B7-H1 Antigen; C423236:CD274 protein, human; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D011994:Recombinant Proteins