title: [EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing.].
abstract: BACKGROUND
  Small molecule tyrosine kinase inhibitors (TKIs), such as gefitinib and erlotinib that target the kinase domain of epidermal growth factor receptor (EGFR) are making successful progression for advanced non-small cell lung cancer patients treatment. The growing evidences revealed that EGFR exon 19 and 21 mutation status in NSCLC patients was correlated with the outcome for EGFR-TKI treatment. In this study, two methods of Real-time PCR and DNA sequencing were compared to detected EGFR exon 19 and 21 mutations.
  
  
  METHODS
  EGFR exon19 mutation del-E746-A750 and exon 21mutation L858R were detected by Real-time PCR and DNA sequencing in 103 NSCLC patients. Chi-square test was used to analyze the consistance.
  
  
  RESULTS
  There was no significant difference between the two methods. However, Real-time PCR was more convenient and sensitive compared to DNA sequencing.
  
  
  CONCLUSIONS
  Real-time PCR was more suitable for clinical testing than DNA sequencing.
authors: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors: Yongwen
authors: Y
authors: Li
authors: Hongyu
authors: H
authors: Liu
authors: Ying
authors: Y
authors: Li
authors: Yuli
authors: Y
authors: Wang
authors: Jing
authors: J
authors: Wang
authors: Jun
authors: J
authors: Chen
authors: Qinghua
authors: Q
authors: Zhou