title: Identification and characterization of phage-displayed peptide mimetics of Neisseria meningitidis serogroup B capsular polysaccharide.
abstract: Neisseria meningitidis causes meningitis and septicemia. There is no single vaccine against all serogroup B meningococcal (MenB) strains up to now. Their capsular polysaccharide (MenB CPS) bears epitopes both cross-reacting and non-cross-reactive with human polysialic acid. A bactericidal and protective antibody mAb (13D9) recognizing a unique epitope in MenB CPS was used to screen a phage-displayed peptide library. Four peptides, able to bind mAb 13D9 in competition with MenB CPS, were identified. Immunization of mice with the phage-displayed peptides elicited anti-peptide IgG antibodies, mainly IgG(2a) for 3 of the peptides and bactericidal and protective antibody levels for one of them. Peptides specifically targeting the immune response toward epitopes found only in MenB CPS could be considered for a universal vaccine against serogroup B meningococcal strains.
authors: Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba. tamara.menendez@cigb.edu.cu
authors: Tamara
authors: T
authors: Menéndez
authors: Nelson F
authors: NF
authors: Santiago-Vispo
authors: Yoelys
authors: Y
authors: Cruz-Leal
authors: Edelgis
authors: E
authors: Coizeau
authors: Hilda
authors: H
authors: Garay
authors: Osvaldo
authors: O
authors: Reyes
authors: Yaima
authors: Y
authors: Batista
authors: Karem
authors: K
authors: Cobas
authors: Tania
authors: T
authors: Carmenate
authors: Glay
authors: G
authors: Chinea
authors: Gerardo
authors: G
authors: Guillén
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D022401:Meningococcal Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D009345:Neisseria meningitidis / Q000276:immunology*; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D051381:Rats; D058248:Serum Bactericidal Antibody Assay
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines; D019151:Peptide Library; D010455:Peptides; D011135:Polysaccharides, Bacterial