Dynamic ray tracing for modeling optical cell manipulation.
Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real and non-rigid cells can deform, so assumptions inherent in traditional ray optics methods begin to break down. In this work, we implement a dynamic ray tracing technique to calculate the stress distribution on a deformable cell induced by optical trapping. Here, cells are modeled as three-dimensional elastic capsules with a discretized surface with associated hydrodynamic forces calculated using the Immersed Boundary Method. We use this approach to simulate the transient deformation of spherical, ellipsoidal and biconcave capsules due to external optical forces induced by a single diode bar optical trap for a range of optical powers.
Sraj
Ihab
I
Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.
Szatmary
Alex C
AC
Marr
David W M
DW
Eggleton
Charles D
CD
D000818:Animals; D002477:Cells; D003198:Computer Simulation; D057446:Hydrodynamics; D008846:Micromanipulation / Q000379:methods*; D008954:Models, Biological; D052898:Optical Tweezers; D012860:Skates, Fish; D013314:Stress, Mechanical