Chemical and thermal cross-linking of collagen and elastin hydrolysates.
Chemical and thermal cross-linking of collagen soluble in acetic acid and elastin hydrolysates soluble in water have been studied. Solutions of collagen and elastin hydrolysates were treated using variable concentrations of 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Moreover, diepoxypropylether (DEPE) has been used as cross-linking agent. Films made of collagen and elastin hydrolysates were also treated with temperature at 60°C and 100°C to get additional cross-links. The effect of cross-linking has been studied using FTIR spectroscopy, thermal analysis, AFM and SEM microscopy. Mechanical and surface properties of materials have been studied after cross-linking. It was found that thermal and mechanical properties of collagen and elastin materials have been altered after thermal treatment and after the reactions with EDC/NHS and/or DEPE. Surface properties of collagen materials after chemical cross-linking have been modified. Thermal and chemical cross-linking of collagen films lead to alteration of polarity of the surface.
Sionkowska
A
A
Nicolaus Copernicus University, Faculty of Chemistry, Gagarin 7, 87-100 Torun, Poland. as@chem.uni.torun.pl
Skopinska-Wisniewska
J
J
Gawron
M
M
Kozlowska
J
J
Planecka
A
A
D000577:Amides / Q000737:chemistry; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D004549:Elastin / Q000378:metabolism*; D004986:Ether / Q000737:chemistry / Q000494:pharmacology; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry / Q000494:pharmacology; D005782:Gels; D018625:Microscopy, Atomic Force; D011492:Protein Hydrolysates / Q000378:metabolism*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013388:Succinimides / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D034421:Sus scrofa; D013696:Temperature; D013718:Tensile Strength / Q000187:drug effects; D013818:Thermogravimetry
D000577:Amides; D003432:Cross-Linking Reagents; D005782:Gels; D011492:Protein Hydrolysates; D013388:Succinimides; D004986:Ether; D003094:Collagen; D004549:Elastin; C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide