title: Chronic alcohol intake upregulates hepatic expression of carotenoid cleavage enzymes and PPAR in rats.
abstract: Excessive and chronic alcohol intake leads to a lower hepatic vitamin A status by interfering with vitamin A metabolism. Dietary provitamin A carotenoids can be converted into vitamin A mainly by carotenoid 15,15'-monooxygenase 1 (CMO1) and, to a lesser degree, carotenoid 9'10'-monooxygenase 2 (CMO2). CMO1 has been shown to be regulated by several transcription factors, such as the PPAR, retinoid X receptor, and thyroid receptor (TR). The regulation of CMO2 has yet to be identified. The impact of chronic alcohol intake on hepatic expressions of CMO1 and CMO2 and their related transcription factors are unknown. In this study, Fischer 344 rats were pair-fed either a liquid ethanol Lieber-DeCarli diet (n = 10) or a control diet (n = 10) for 11 wk. Hepatic retinoid concentration and expressions of CMO1, CMO2, PPARγ, PPARα, and TRβ as well as plasma thyroid hormones levels were analyzed. We observed that administering alcohol decreased hepatic retinoid levels but increased mRNA concentrations of CMO1, CMO2, PPARγ, PPARα, and TRβ and upregulated protein levels of CMO2, PPARγ, and PPARα. There was a positive correlation of PPARγ with CMO1 (r = 0.89; P < 0.0001) and both PPARγ and PPARα with CMO2 (r = 0.72, P < 0.001 and r = 0.62, P < 0.01, respectively). Plasma thyroid hormone concentrations did not differ between the control rats and alcohol-fed rats. This study suggests that chronic alcohol intake significantly upregulates hepatic expression of CMO1 and, to a much lesser extent, CMO2. This process may be due to alcohol-induced PPARγ expression and lower vitamin A status in the liver.
authors: Nutrition and Cancer Biology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
authors: Renata A M
authors: RA
authors: Luvizotto
authors: André F
authors: AF
authors: Nascimento
authors: Sudipta
authors: S
authors: Veeramachaneni
authors: Chun
authors: C
authors: Liu
authors: Xiang-Dong
authors: XD
authors: Wang
mesh_terms: D000818:Animals; D000431:Ethanol / Q000008:administration & dosage*; D044943:Fatty Acid Desaturases / Q000235:genetics; D008099:Liver / Q000737:chemistry / Q000201:enzymology*; D008297:Male; D047493:PPAR alpha / Q000032:analysis / Q000235:genetics; D047495:PPAR gamma / Q000032:analysis / Q000235:genetics; D047492:Peroxisome Proliferator-Activated Receptors / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D012176:Retinoids / Q000032:analysis; D037042:Thyroid Hormone Receptors beta / Q000032:analysis / Q000235:genetics; D013963:Thyroid Hormones / Q000097:blood; D015854:Up-Regulation / Q000187:drug effects*; D050559:beta-Carotene 15,15'-Monooxygenase / Q000235:genetics*
chemical_list: D047493:PPAR alpha; D047495:PPAR gamma; D047492:Peroxisome Proliferator-Activated Receptors; D012333:RNA, Messenger; D012176:Retinoids; D037042:Thyroid Hormone Receptors beta; D013963:Thyroid Hormones; D000431:Ethanol; D044943:Fatty Acid Desaturases; C552882:carotenoid 9',10'-monooxygenase 2, rat; D050559:beta-Carotene 15,15'-Monooxygenase