Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV- CNE2 cells.
OBJECTIVE
The aim of this study was to compare the sensitivity of hematoporphyrin monomethyl ether (HMME)-mediated PDT in the Epstein-Barr virus (EBV) positive and negative human nasopharyngeal carcinoma (NPC) cells.


METHODS
The intracellular uptake of HMME in C666-1 (EBV+) and CNE2 (EBV-) cells was quantitatively determined after incubating with different concentration of HMME. The subcellular localization of HMME was examined under a laser scanning fluorescence microscopy. The survival rate of C666-1 and CNE2 cells after HMME PDT was evaluated with clonogenic assay.


RESULTS
The EBV- CNE2 cells showed higher intracellular uptake of HMME and lower survival rate than EBV+ C666-1 cells. There was no significant difference in terms of subcellular localization of HMME between C666-1 and CNE2 cells.


CONCLUSIONS
The sensitivity of EBV- CNE2 cells to HMME-mediated PDT was significantly higher than that of EBV+ C666-1 cells. The intracellular uptake of HMME plays a key role in determining the PDT sensitivity.
Li
Buhong
B
Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China. bhli@fjnu.edu.cn
Chen
Zheng
Z
Liu
Lina
L
Huang
Zufang
Z
Huang
Zheng
Z
Xie
Shusen
S
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006415:Hematoporphyrins / Q000493:pharmacokinetics / Q000494:pharmacology*; D004854:Herpesvirus 4, Human; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology*
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C494379:hematoporphyrin monomethyl ether