title: Bending the rules of transcriptional repression: tightly looped DNA directly represses T7 RNA polymerase.
abstract: From supercoiled DNA to the tight loops of DNA formed by some gene repressors, DNA in cells is often highly bent. Despite evidence that transcription by RNA polymerase (RNAP) is affected in systems where DNA is deformed significantly, the mechanistic details underlying the relationship between polymerase function and mechanically stressed DNA remain unclear. Seeking to gain additional insight into the regulatory consequences of highly bent DNA, we hypothesize that tightly looping DNA is alone sufficient to repress transcription. To test this hypothesis, we have developed an assay to quantify transcription elongation by bacteriophage T7 RNAP on small, circular DNA templates approximately 100 bp in size. From these highly bent transcription templates, we observe that the elongation velocity and processivity can be repressed by at least two orders of magnitude. Further, we show that minicircle templates sustaining variable levels of twist yield only moderate differences in repression efficiency. We therefore conclude that the bending mechanics within the minicircle templates dominate the observed repression. Our results support a model in which RNAP function is highly dependent on the bending mechanics of DNA and are suggestive of a direct, regulatory role played by the template itself in regulatory systems where DNA is known to be highly bent.
authors: Cellular and Molecular Biology Program, Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
authors: Troy A
authors: TA
authors: Lionberger
authors: Edgar
authors: E
authors: Meyhöfer
mesh_terms: D001681:Biological Assay; D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry / Q000648:ultrastructure; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D009690:Nucleic Acid Conformation; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004270:DNA, Circular; D014764:Viral Proteins; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases