Discordant expression of miR-103/7 and pantothenate kinase host genes in mouse.
miR-103 and miR-107, microRNAs hosted by pantothenate kinase genes, are proposed to regulate cellular lipid metabolism. microRNA-mediated regulation is complex, potentially affecting expression of the host gene, related enzymes within the same pathway, or apparently distinct targets. Using qRT-PCR, we demonstrate that miR-103 and miR-107 expression does not correlate with expression of host pantothenate kinase genes in mouse tissues. The miR-103/7 family thus provides an intriguing model for dissecting microRNA transcription, processing and coordinated function within host genes.
Polster
Brenda J
BJ
Molecular and Medical Genetics, Oregon Health & Science University, Portland OR, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA. polsterb@ohsu.edu
Westaway
Shawn K
SK
Nguyen
Thuy M
TM
Yoon
Moon Y
MY
Hayflick
Susan J
SJ
D000818:Animals; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C555364:MIRN103 microRNA, mouse; C555365:MIRN107 microRNA, mouse; D035683:MicroRNAs; D017853:Phosphotransferases (Alcohol Group Acceptor); C023088:pantothenate kinase