ChIP-seq and Functional Analysis of the SOX2 Gene in Colorectal Cancers.
Abstract   SOX2 is a high mobility group (HMG) box containing transcription factor that has been implicated in various types of cancer, but its role in colorectal cancers (CRC) has not been studied. Here we show that SOX2 is overexpressed in CRC tissues compared with normal adjacent tissues using immunohistochemical staining and RT-PCR. We also observed an increased SOX2 expression in nucleus of colorectal cancer tissues (46%, 14/30 cases vs. 7%, 2/30 adjacent tissues). Furthermore, knockdown of SOX2 in SW620 colorectal cancer cells decreased their growth rates in vitro cell line, and in vivo in xenograft models. ChIP-seq analysis of SOX2 revealed a consensus sequence of wwTGywTT. An integrated expression profiling and ChIP-seq analysis show that SOX2 is involved in the BMP signaling pathway, steroid metabolic process, histone modifications, and many receptor-mediated signaling pathways such as IGF1R and ITPR2 (Inositol 1,4,5-triphosphate receptor, type 2).
Fang
Xuefeng
X
1 Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital,  Zhejiang University School of Medicine , Hangzhou, Zhejiang,  People's Republic of China .
Yu
Wei
W
Li
Lisha
L
Shao
Jiaofang
J
Zhao
Na
N
Chen
Qiyun
Q
Ye
Zhiyun
Z
Lin
Sheng-Cai
SC
Zheng
Shu
S
Lin
Biaoyang
B