title: Human progenitor cells derived from cardiac adipose tissue ameliorate myocardial infarction in rodents.
abstract: Myocardial infarction caused by vascular occlusion results in the formation of nonfunctional fibrous tissue. Cumulative evidence indicates that cell therapy modestly improves cardiac function; thus, novel cell sources with the potential to repair injured tissue are actively sought. Here, we identify and characterize a cell population of cardiac adipose tissue-derived progenitor cells (ATDPCs) from biopsies of human adult cardiac adipose tissue. Cardiac ATDPCs express a mesenchymal stem cell-like marker profile (strongly positive for CD105, CD44, CD166, CD29 and CD90) and have immunosuppressive capacity. Moreover, cardiac ATDPCs have an inherent cardiac-like phenotype and were able to express de novo myocardial and endothelial markers in vitro but not to differentiate into adipocytes. In addition, when cardiac ATDPCs were transplanted into injured myocardium in mouse and rat models of myocardial infarction, the engrafted cells expressed cardiac (troponin I, sarcomeric α-actinin) and endothelial (CD31) markers, vascularization increased, and infarct size was reduced in mice and rats. Moreover, significant differences between control and cell-treated groups were found in fractional shortening and ejection fraction, and the anterior wall remained significantly thicker 30days after cardiac delivery of ATDPCs. Finally, cardiac ATDPCs secreted proangiogenic factors under in vitro hypoxic conditions, suggesting a paracrine effect to promote local vascularization. Our results indicate that the population of progenitor cells isolated from human cardiac adipose tissue (cardiac ATDPCs) may be valid candidates for future use in cell therapy to regenerate injured myocardium.
authors: Laboratory of Cell Physiology, Department of Cardiology, Hospital de la Santa Creu i Sant Pau, ICCC, Barcelona, Spain. abayes.germanstrias@gencat.cat
authors: Antoni
authors: A
authors: Bayes-Genis
authors: Carolina
authors: C
authors: Soler-Botija
authors: Jordi
authors: J
authors: Farré
authors: Pilar
authors: P
authors: Sepúlveda
authors: Angel
authors: A
authors: Raya
authors: Santiago
authors: S
authors: Roura
authors: Cristina
authors: C
authors: Prat-Vidal
authors: Carolina
authors: C
authors: Gálvez-Montón
authors: José Anastasio
authors: JA
authors: Montero
authors: Dirk
authors: D
authors: Büscher
authors: Juan Carlos
authors: JC
authors: Izpisúa Belmonte
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000368:Aged; D043925:Angiogenesis Inducing Agents / Q000378:metabolism; D000818:Animals; D002196:Capillaries / Q000473:pathology; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006334:Heart Function Tests; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000166:cytology*; D018919:Neovascularization, Physiologic; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014463:Ultrasonography
chemical_list: D043925:Angiogenesis Inducing Agents