title: Use of bovine hydroxyapatite with or without biomembrane in sinus lift in rabbits: histopathologic analysis and immune expression of core binding factor 1 and vascular endothelium growth factor.
abstract: OBJECTIVE
  Considering the clinical discussion on the necessity of using a barrier membrane in the osteotomy area of sinus lift procedures to prevent fibrous tissue formation in this area and as a physical limit, the aim of this study was to analyze and compare the use of bovine hydroxyapatite (HA) with and without a biologic membrane by histopathologic analysis and immune expression of core binding factor 1 and vascular endothelium growth factor in the sinus lift in rabbits.
  
  
  METHODS
  Sixteen male rabbits underwent bilateral sinus lift procedures and were divided into 2 groups according to the sinus filling material: group 1 received bovine HA (Bio-Oss; Geistlich Pharma AG, Wohlhusen, Switzerland) and group 2 received bovine HA and a nonporous polytetrafluorethylene membrane. All groups were sacrificed after 7, 14, 30, and 60 days for microscopic, histomorphometric, and immunohistochemical analyses.
  
  
  RESULTS
  Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft tissue, and the remaining material were analyzed by histomorphometric analysis. No statistically significant differences (P > .05) were detected between groups for all periods analyzed. In addition, no remarkable differences were noticed in core binding factor 1 or vascular endothelium growth factor immune expression.
  
  
  CONCLUSIONS
  Taken together, these results show that using a biologic membrane does not improve bone repair induced by bovine HA, as shown by histopathologic and immunohistochemical analyses.
authors: Department of Health Sciences, Discipline of Oral and Maxillofacial Surgery, School of Dentistry, Sagrado Coração University, Bauru, SP, Brazil.
authors: Leandro Soeiro
authors: LS
authors: De Souza Nunes
authors: Renato Victor
authors: RV
authors: De Oliveira
authors: Leandro Andrade
authors: LA
authors: Holgado
authors: Hugo
authors: H
authors: Nary Filho
authors: Daniel Araki
authors: DA
authors: Ribeiro
authors: Mariza Akemi
authors: MA
authors: Matsumoto
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001857:Bone Matrix / Q000637:transplantation; D018786:Bone Substitutes / Q000627:therapeutic use*; D002417:Cattle; D003238:Connective Tissue / Q000473:pathology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis*; D017886:Durapatite / Q000627:therapeutic use*; D006097:Granulation Tissue / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008437:Maxilla / Q000473:pathology / Q000601:surgery*; D008443:Maxillary Sinus / Q000473:pathology / Q000601:surgery*; D008567:Membranes, Artificial; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis / Q000502:physiology; D010027:Osteotomy / Q000379:methods; D011138:Polytetrafluoroethylene; D011817:Rabbits; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D008567:Membranes, Artificial; D008903:Minerals; D042461:Vascular Endothelial Growth Factor A; D011138:Polytetrafluoroethylene; D017886:Durapatite