Platelets prevent acute liver damage after extended hepatectomy in pigs.
OBJECTIVE
Platelets develop tissue repair and promote liver regeneration. We investigated whether platelets prevented acute liver damage after extended hepatectomy in pigs.


METHODS
Thrombocytosis was induced by the following two methods; afterwards 80% hepatectomy was performed in pigs. In the first method, the pigs received administration of thrombopoietin [TPO (+) group], and they were compared with a control group [TPO (-) group]. In the second method, the pigs received a splenectomy [Sp (+) group], and theywere compared with another control group [Sp (-) group]. Platelet counts, biochemical examination of blood, and histopathological findings of the residual liver were examined.


RESULTS
Serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin (T-Bil) levels were significantly decreased in the thrombocytotic groups compared with the control groups in the early period after hepatectomy. In the histopathological findings, hemorrhagic necrosis with a bile plug was observed in the control groups, but this phenomenon was not observed in the thrombocytotic groups. On transmission electron microscopy, the sinusoidal endothelial lining was destroyed and detached into the sinusoidal space with enlargement of Disse's spaces in the thrombocytotic groups, but these findings were not observed in the control groups.


CONCLUSIONS
An increased number of platelets prevents acute liver damage after extended hepatectomy.
Hisakura
Katsuji
K
Department of Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8575, Japan.
Murata
Soichiro
S
Fukunaga
Kiyoshi
K
Myronovych
Andriy
A
Tadano
Sosuke
S
Kawasaki
Takuya
T
Kohno
Keisuke
K
Ikeda
Osamu
O
Pak
Sugiru
S
Ikeda
Naoya
N
Nakano
Yoritaka
Y
Matsuo
Ryota
R
Konno
Kenjiro
K
Kobayashi
Eiji
E
Saito
Toshiyuki
T
Yasue
Hiroshi
H
Ohkohchi
Nobuhiro
N
D055371:Acute Lung Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001792:Blood Platelets / Q000502:physiology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006498:Hepatectomy / Q000009:adverse effects*; D010976:Platelet Count; D013156:Splenectomy / Q000379:methods; D013552:Swine; D013556:Swine, Miniature; D013922:Thrombocytosis / Q000097:blood*; D013926:Thrombopoietin / Q000008:administration & dosage / Q000627:therapeutic use*; D016896:Treatment Outcome
D013926:Thrombopoietin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin