title: Method for umbilical cord blood-derived basophils by FCM.
abstract: We have devised a method for the purification of human basophils from umbilical cord blood by FCM. Umbilical cord blood was collected from six healthy, full-term deliveries. After separation of red blood cells, mononuclear cells were isolated by Ficoll-Hypaque. Six samples followed by positive selection using flowcytometry (FCM) for CD203c(+)CD45(int+) cells. Purity and recovery of cells were measured. Purity and recovery of basophils by FCM with CD203c(+)CD45(int+) markers were 95.02 +/- 2.94% and 61.42 +/- 5.95%. Cell sorting for CD203c(+)CD45(int+) cells by FCM is an improved method for obtaining pure umbilical cord blood-derived basophils. We established cord blood-derived basophil purification technique as a source from which active basophils can be isolated for immunochemical characterization.
authors: Respiratory Department, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Ling
authors: L
authors: Yang
authors: Wei-Guo
authors: WG
authors: Xu
authors: Yi-Ping
authors: YP
authors: Xu
authors: Su-Hang
authors: SH
authors: Yao
authors: Yin-Shi
authors: YS
authors: Guo
authors: Ke-Min
authors: KM
authors: Wang
authors: Ying
authors: Y
authors: Xiong
authors: Jie
authors: J
authors: Zhu
mesh_terms: D001491:Basophils / Q000166:cytology* / Q000502:physiology; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology*; D005362:Ficoll / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000378:metabolism; D011755:Pyrophosphatases / Q000276:immunology / Q000378:metabolism
chemical_list: C427944:ENPP3 protein, human; D005362:Ficoll; D017493:Leukocyte Common Antigens; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases