title: NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
abstract: NfrA1 nitroreductase from the Gram-positive bacterium Bacillus subtilis is a member of the NAD(P)H/FMN oxidoreductase family. Here, we investigated the reactivity, the structure and kinetics of NfrA1, which could provide insight into the unclear biological role of this enzyme. We could show that NfrA1 possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide. Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously.
authors: Institut de Chimie des Substances Naturelles (ICSN), CNRS UPR 2301, Gif-sur-Yvette, France.
authors: Sylvie
authors: S
authors: Cortial
authors: Philippe
authors: P
authors: Chaignon
authors: Bogdan I
authors: BI
authors: Iorga
authors: Stéphane
authors: S
authors: Aymerich
authors: Gilles
authors: G
authors: Truan
authors: Virginie
authors: V
authors: Gueguen-Chaignon
authors: Philippe
authors: P
authors: Meyer
authors: Solange
authors: S
authors: Moréra
authors: Jamal
authors: J
authors: Ouazzani
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009536:Niacinamide / Q000096:biosynthesis; D009601:Nitroreductases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D011487:Protein Conformation; D013481:Superoxides / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D009243:NAD; D013481:Superoxides; D009536:Niacinamide; D006861:Hydrogen Peroxide; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C116226:NfrA1 protein, Bacillus subtilis