title: The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput.
abstract: Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.
authors: Technology Development Team, Center for Top Down Proteomics, University of Illinois at Urbana-Champaign, USA.
authors: John F
authors: JF
authors: Kellie
authors: John C
authors: JC
authors: Tran
authors: Ji Eun
authors: JE
authors: Lee
authors: Dorothy R
authors: DR
authors: Ahlf
authors: Haylee M
authors: HM
authors: Thomas
authors: Ioanna
authors: I
authors: Ntai
authors: Adam D
authors: AD
authors: Catherman
authors: Kenneth R
authors: KR
authors: Durbin
authors: Leonid
authors: L
authors: Zamdborg
authors: Adaikkalam
authors: A
authors: Vellaichamy
authors: Paul M
authors: PM
authors: Thomas
authors: Neil L
authors: NL
authors: Kelleher
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D011506:Proteins