[Molecular basis for real RhD negative and RhDel phenotypes in Yiwu population of Zhejiang Province in China].
This study was purposed to investigate the molecular basis for RhD negative phenotype in Yiwu population in Zhejiang Province of China. The RhD negative samples were screened by saline agglutination test in blood donors. Some real RhD negative and RhDel phenotypes were identified using anti-human globulin test and absorbtion elution test. Ten exons of RHD gene in these samples were amplified by PCR-SSP, and positive exons were DNA sequenced. The results indicated that 30 real RhD negative and 8 RhDel phenotypes were identified in 38 initial RhD negative samples. Ten exons were complete negative in 28 real RhD negative samples and only exon 1, 2 and 10 were positive in 2 real RhD negative samples amplified by PCR. All 10 exons in 8 RbDel samples were positive and a DNA variant (1227G > A) was found in 8 RhDel samples. It is concluded that all exons are absence in most real RhD negative phenotypes, and the partial exons absence is also found in some real negative phenotypes among Yiwu population in Zhejiang province of China. The G to A mutation at position 1227 is found in all RhDel phenotypes.
Jin
Xiao-Dong
XD
Central Blood Station of Yiwu, Yiwu 322000, Zhejiang Province, China.
Fu
Guang-Cheng
GC
Xu
Xian-Guo
XG
Zhu
Fa-Ming
FM
Yan
Li-Xing
LX
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002681:China; D005091:Exons; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011110:Polymorphism, Genetic; D012204:Rh-Hr Blood-Group System / Q000235:genetics* / Q000276:immunology
D012204:Rh-Hr Blood-Group System