title: Induction of endothelial nitric oxide synthase, SIRT1, and catalase by statins inhibits endothelial senescence through the Akt pathway.
abstract: OBJECTIVE
  Statins (3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors) have pleiotropic vascular protective effects besides cholesterol lowering. Recently, experimental and clinical studies have indicated that senescence of endothelial cells is involved in endothelial dysfunction and atherogenesis. Therefore, the present study was performed to determine whether statins would reduce endothelial senescence and to clarify the molecular mechanisms underlying the antisenescent property of statins.
  
  
  RESULTS
  Senescent human umbilical vein endothelial cells were induced by hydrogen peroxide (H(2)O(2)), as judged by senescence-associated β-galactosidase assay and cell morphological appearance. Atorvastatin, pravastatin, and pitavastatin inhibited the oxidative stress induced-endothelial senescence. These statins phosphorylated Akt at Ser473 and subsequently led to increased expression of endothelial nitric oxide synthase (eNOS), SIRT1, and catalase. Treatment with LY294002 or Akt short interfering RNA decreased the eNOS activation, SIRT1 expression, and antisenescent property of atorvastatin. Moreover, in streptozotocin-diabetic mice, administration of pitavastatin increased eNOS, SIRT1, and catalase expression and decreased endothelial senescence, but levels remained unaltered in Sirt1 knockout mice.
  
  
  CONCLUSIONS
  Our results indicate that treatment with statins inhibits endothelial senescence and that enhancement of SIRT1 plays a critical role in prevention of endothelial senescence through the Akt pathway, a direct target of statins.
authors: Department of Geriatric Medicine, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Hidetaka
authors: H
authors: Ota
authors: Masato
authors: M
authors: Eto
authors: Mitsunobu R
authors: MR
authors: Kano
authors: Tomoaki
authors: T
authors: Kahyo
authors: Mitsutoshi
authors: M
authors: Setou
authors: Sumito
authors: S
authors: Ogawa
authors: Katsuya
authors: K
authors: Iijima
authors: Masahiro
authors: M
authors: Akishita
authors: Yasuyoshi
authors: Y
authors: Ouchi
mesh_terms: D000818:Animals; D000069059:Atorvastatin; D002374:Catalase / Q000096:biosynthesis*; D016922:Cellular Senescence / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects; D006538:Heptanoic Acids / Q000494:pharmacology; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects; D017035:Pravastatin / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D011758:Pyrroles / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D056564:Sirtuin 1 / Q000096:biosynthesis / Q000502:physiology*; D014471:Umbilical Veins
chemical_list: D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D011804:Quinolines; D000069059:Atorvastatin; D002374:Catalase; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D051057:Proto-Oncogene Proteins c-akt; D056564:Sirtuin 1; D017035:Pravastatin; C108475:pitavastatin