title: Establishment and phenotypic characterization of the first human pulmonary blastoma cell line.
abstract: BACKGROUND
  Sarcomatoid carcinomas are a group of poorly differentiated carcinomas containing a sarcomatoid component with the presence of epithelial-mesenchymal transition.
  
  
  METHODS
  To obtain a stabilized cell line, three mediums were used: IMDM, BEBM and IMDM/BEBM. CD44, CD29, CD90, CD133, CD326 antigens, cytokeratins and vimentin were tested by flow cytometry and immunofluorescence assay. Side population, spheres formation, tumorigenicity in vitro and in vivo and cell cycle analysis were analyzed.
  
  
  RESULTS
  At day of surgery, cytometric analysis revealed that CD133, CD90 and CD326 levels were very low, CD29 and CD44 were about 80%. After 30 days of culture, CD133 levels increased up to 30%, all cells expressed CD90 and vimentin markers and CD326 was lost. The cells grew only in IMDM/BEBM medium. The cell population was heterogeneous with epithelial and mesenchymal cells. After about 15-30 days, only fibroblast-like cells were observed. LC114 cells were not able to grow as spheres and they did not show a side-population phenotype. The cell cycle analysis confirmed an aneuploid population in the tissue and normal diploid population in cell line.
  
  
  CONCLUSIONS
  We selected, characterized and stabilized a primary cell line of human pulmonary blastoma by the expression both of stemness and differentiation markers and confirmed the presence of the marker CD133.
authors: Department of Experimental Oncology, National Cancer Institute, Via Mariano Semmola, 80131 Naples, Italy.
authors: Rosa
authors: R
authors: Camerlingo
authors: Renato
authors: R
authors: Franco
authors: Virginia
authors: V
authors: Tirino
authors: Monica
authors: M
authors: Cantile
authors: Mariano
authors: M
authors: Rocchi
authors: Antonello
authors: A
authors: La Rocca
authors: Nicola
authors: N
authors: Martucci
authors: Gerardo
authors: G
authors: Botti
authors: Gaetano
authors: G
authors: Rocco
authors: Giuseppe
authors: G
authors: Pirozzi
mesh_terms: D000369:Aged, 80 and over; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D018202:Pulmonary Blastoma / Q000378:metabolism / Q000473:pathology*; D018874:Spheroids, Cellular; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD