title: [Determination of chlorogenic acid, caffeic acid and linarin in Flos Chrysanthemi Indici from different places by RP-hPLC].
abstract: OBJECTIVE
  To evaluate the quality of Flos Chrysanthemi Indici which produced in twenty-two different producing places.
  
  
  METHODS
  Chlorogenic acid and caffeic acid were analyzed on a Shim-pack C8 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-0.5% phosphoric acid( 19:81). The detection wavelength was set at 326 nm. Linarin were eluted with the mobile phase consisted of methanol-water-acetic acid(26: 23: 1). The detection wavelength was set at 334 nm. The column temperature was 25 degrees C. The flow rate was 1.0 mL x min .
  
  
  RESULTS
  The linear response ranged within 2.5-50 microg for chlorogenic acid (r = 0.998), 2.5-25 microg for caffeic acid (r = 0.998) and 4.97-41.47 microg for linarin (r = 0.999), respectively. Recoveries were 100.8% with RSD 2.1% for chlorogenic acid, 96.2% with RSD 2.3% for caffeic acid and 103.7% with RSD 1.8% for linarin.
  
  
  CONCLUSIONS
  There was a significant difference in the content of chlorogenic acid, caffeic acid, linarin among the samples. The content of chlorogenic in the sample from Fengdou Chongqing city was the highest in those from other places. The content of caffeic acid in the all samples is very low. The content of linarin in the samples from Jiangsu province and Anhui province almost reached the national standard in pharmacopoeia.
authors: Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China. gqs@njau.edu.cn
authors: Qiaosheng
authors: Q
authors: Guo
authors: Hailing
authors: H
authors: Fang
authors: Haijin
authors: H
authors: Shen
mesh_terms: D002109:Caffeic Acids / Q000032:analysis*; D002681:China; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D006027:Glycosides / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D011786:Quality Control
chemical_list: D002109:Caffeic Acids; D006027:Glycosides; D010936:Plant Extracts; D002726:Chlorogenic Acid; C008282:linarin; C040048:caffeic acid