title: Kinetics of angiogenic changes in a new mouse model for hepatocellular carcinoma.
abstract: BACKGROUND
  The increasing incidence of hepatocellular carcinoma in Western countries has led to an expanding interest of scientific research in this field. Therefore, a vast need of experimental models that mimic the natural pathogenesis of hepatocellular carcinoma (HCC) in a short time period is present. The goal of our study was (1) to develop an efficient mouse model for HCC research, in which tumours develop in a natural background of fibrosis and (2) to assess the time-dependent angiogenic changes in the pathogenesis of HCC.
  
  
  METHODS
  Weekly intraperitoneal injections with the hepatocarcinogenic compound N-nitrosodiethylamine was applied as induction method and samples were taken at several time points to assess the angiogenic changes during the progression of HCC.
  
  
  RESULTS
  The N-nitrosodiethylamine-induced mouse model provides well vascularised orthotopic tumours after 25 weeks. It is a representative model for human HCC and can serve as an excellent platform for the development of new therapeutic targets.
authors: Department of Gastroenterology and Hepatology, Ghent University Hospital, Ghent, Belgium.
authors: Femke
authors: F
authors: Heindryckx
authors: Koen
authors: K
authors: Mertens
authors: Nicolas
authors: N
authors: Charette
authors: Bert
authors: B
authors: Vandeghinste
authors: Christophe
authors: C
authors: Casteleyn
authors: Christophe
authors: C
authors: Van Steenkiste
authors: Dominique
authors: D
authors: Slaets
authors: Louis
authors: L
authors: Libbrecht
authors: Steven
authors: S
authors: Staelens
authors: Peter
authors: P
authors: Starkel
authors: Anja
authors: A
authors: Geerts
authors: Isabelle
authors: I
authors: Colle
authors: Hans
authors: H
authors: Van Vlierberghe
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D004052:Diethylnitrosamine / Q000633:toxicity; D004195:Disease Models, Animal; D008114:Liver Neoplasms, Experimental / Q000098:blood supply* / Q000139:chemically induced; D051379:Mice; D009389:Neovascularization, Pathologic
chemical_list: D002273:Carcinogens; D004052:Diethylnitrosamine