title: [Promotion effect of stromal cell-derived factor 1 on the migration of epidermal stem cells in the healing process of frostbite-wound model ex vivo].
abstract: OBJECTIVE
  To study the promotion effect of stromal cell-derived factor 1 (SDF-1) on the migration of epidermal stem cells (ESC) in the healing process of frostbite-wound model ex vivo.
  
  
  METHODS
  A three-dimensional model of full-thickness frostbite of skin was constructed (with slot-like wound) out of skin equivalent. The expression of SDF-1 in wound stroma was observed with immunohistochemistry staining on post injury days (PID) 3 and 7. The model frostbite wounds were divided into control group (treated with PBS 50 microL per wound), SDF-1 group (treated with 100 ng/mL SDF-1, 50 microL per wound), and AMD3100 group [treated with 100 ng/mL AMD3100 (50 microL per wound) for 30 minutes, and then SDF-1 50 microL was added per wound]. The redistribution of ESC around wound was observed.
  
  
  RESULTS
  The expression of SDF-1 in wound stroma increased gradually on PID 3 and 7. Compared with those in control and AMD3100 groups, there were more ESC and epithelial cell layers, and more integrin beta(1)-positive cells appeared at the basal layer of wound in SDF-1 group, and some of the positive cells migrated upward to epidermis.
  
  
  CONCLUSIONS
  SDF-1 contributes to wound repair through promoting ESC to migrate toward and gather around wound edge. This may be one of the mechanisms of ESC participating in wound repair.
authors: Department of Plastic Surgery, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors: Lu
authors: L
authors: Gan
authors: Chuan
authors: C
authors: Cao
authors: Shi-rong
authors: SR
authors: Li
authors: Lin-lin
authors: LL
authors: Chai
authors: Rui
authors: R
authors: Guo
authors: Guang-jin
authors: GJ
authors: Xiang
authors: Shu-wen
authors: SW
authors: Zhao
mesh_terms: D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism*; D000078404:Epidermal Cells; D005627:Frostbite / Q000378:metabolism / Q000628:therapy*; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D014945:Wound Healing
chemical_list: D054377:Chemokine CXCL12