[Apoptosis effects on human esophageal cancer cells by soyasaponin Bb and its machanism].
OBJECTIVE
To explore the effect and potential mechanism of apoptosis in the Eca-9706 cells induced by soyasaponin Bb.


METHODS
Different concentrations of soyasaponin Bb were on the Eca-9706 cell. Cell growth suppression rate was detected by MTT. Apoptotic rate was detected by TUNEL. The expression changes of HDAC1, NF-kappaB, PTEN, cyclin D1, c-met, VEGF or caspase-3 in the SSBb were detected by immunohistochemistry and immunoblotting.


RESULTS
Compared with the control group, the growth-inhibition rate and apoptotic rate were increased, the expressions of PTEN, caspase 3 were increased, the expressions of c-met, VEGF, HDAC1, NF-kappaB and cyclin D1 were decreased.


CONCLUSIONS
SSBb can suppress Eca-9706 cell growth. SSBb can exhibit reverse effects on over expression of c-met, VEGF in Eca-9706 cells. Eca-9706 cell apoptosis can be induced by SSBb through inhibiting HDAC1-NF-kappaB and activating PETEN and caspase-3 signaling pathways.
Pei
Yingxin
Y
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Zhao
Huanhuan
H
Du
Xiaoyan
X
Li
Jinping
J
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*
D012503:Saponins; C089346:soyasaponin beta g; C407096:soyasapogenol B; D009828:Oleanolic Acid