The influence of surfactants on cell surface properties of Aeromonas hydrophila during diesel oil biodegradation.
In this study the capacity of the newly isolated environmental strain Aeromonas hydrophila was evaluated. The influence of three surfactants: rhamnolipides, saponins and Triton X-100 on cell surface properties of the A. hydrophila environmental strain and the biodegradation process of diesel oil was studied. The surface activities in water, a mineral salts medium and in the biological system of all considered surfactants were estimated by means of equilibrium surface tension experiments. The obtained results indicated that critical micellar concentration in the biological system is twice higher for saponins and Triton X-100, and three times higher for rhamnolipides. Our results indicated also, that cell surface hydrophobicity (CSH) of bacteria is correlated with carbon sources in broth medium. The mechanism of surfactant action seems to be dependent on the type and concentration of surfactant used in the studies. The best effect of saponins on diesel oil biodegradation was observed using the A. hydrophila strain, diesel oil biodegradation after 21 days was 78%.
Kaczorek
E
E
Institute of Chemical Technology and Engineering, Poznan University of Technology, Pl M Skłodowskiej-Curie 2, 60-965 Poznań, Poland.
Urbanowicz
M
M
Olszanowski
A
A
D016980:Aeromonas hydrophila / Q000737:chemistry / Q000378:metabolism*; D000465:Algorithms; D001422:Bacterial Adhesion / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005742:Gasoline / Q000032:analysis*; D006017:Glycolipids / Q000737:chemistry / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D008956:Models, Chemical; D017830:Octoxynol / Q000737:chemistry / Q000494:pharmacology; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology; D012988:Soil Microbiology; D013499:Surface Properties / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors
D005742:Gasoline; D006017:Glycolipids; D012503:Saponins; D013501:Surface-Active Agents; C418382:rhamnolipid; D017830:Octoxynol