title: Characterization of major histocompatibility complex (MHC) DRB exon 2 and DRA exon 3 fragments in a primary terrestrial rabies vector (Procyon lotor).
abstract: The major histocompatibility complex (MHC) presents a unique system to explore links between genetic diversity and pathogens, as diversity within MHC is maintained in part by pathogen driven selection. While the majority of wildlife MHC studies have investigated species that are of conservation concern, here we characterize MHC variation in a common and broadly distributed species, the North American raccoon (Procyon lotor). Raccoons host an array of broadly distributed wildlife diseases (e.g., canine distemper, parvovirus and raccoon rabies virus) and present important human health risks as they persist in high densities and in close proximity to humans and livestock. To further explore how genetic variation influences the spread and maintenance of disease in raccoons we characterized a fragment of MHC class II DRA exon 3 (250 bp) and DRB exon 2 (228 bp). MHC DRA was found to be functionally monomorphic in the 32 individuals screened; whereas DRB exon 2 revealed 66 unique alleles among the 246 individuals screened. Between two and four alleles were observed in each individual suggesting we were amplifying a duplicated DRB locus. Nucleotide differences between DRB alleles ranged from 1 to 36 bp (0.4-15.8% divergence) and translated into 1 to 21 (1.3-27.6% divergence) amino acid differences. We detected a significant excess of nonsynonymous substitutions at the peptide binding region (P = 0.005), indicating that DRB exon 2 in raccoons has been influenced by positive selection. These data will form the basis of continued analyses into the spatial and temporal relationship of the raccoon rabies virus and the immunogenetic response in its primary host.
authors: Environmental and Life Sciences Gradate Program, Trent University, Peterborough, Ontario, Canada. scastillo@nrdpfc.ca
authors: Sarrah
authors: S
authors: Castillo
authors: Vythegi
authors: V
authors: Srithayakumar
authors: Vanessa
authors: V
authors: Meunier
authors: Christopher J
authors: CJ
authors: Kyle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001499:Bayes Theorem; D004199:Disease Vectors; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D006684:HLA-DR Antigens / Q000737:chemistry / Q000235:genetics*; D059805:HLA-DR alpha-Chains; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000635:transmission*; D011821:Raccoons / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D006684:HLA-DR Antigens; D059805:HLA-DR alpha-Chains