Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa.
BACKGROUND
To find out an alternative strategy to antibiotic usage against bacterial infection.


METHODS
The purpose of this study is to describe the quorum-sensing (QS) inhibitory activity of edible plants and fruits against N-acyl-homoserine lactone (AHL)-mediated violacein production in Chromobacterium violaceum and virulence factor expression in Pseudomonas aeruginosa PAO1.


RESULTS
Aqueous extracts of Ananas comosus (Bromeliaceae), Musa paradiciaca (Musaceae), Manilkara zapota (Sapotaceae) and Ocimum sanctum (Lamiaceae) were prepared and anti-QS activity of each extract was tested against AHL-mediated phenotypic expressions of C. violaceum and PAO1. Most of these extracts showed significant reduction in AHL-mediated violacein production in C. violaceum as well as pyocyanin pigment, staphylolytic protease, elastase production and biofilm formation in PAO1. However, these extracts were not inhibitory to bacterial growth, revealing that the QS inhibition by the extracts is not related to static or killing effects on the bacteria.


CONCLUSIONS
The present study identified the anti-QS activity of A. comosus, M. paradiciaca, M. zapota and O. sanctum. An AHL-inactivating compound from these plant sources can be used as an alternative to antibiotic compounds to prevent AHL-mediated bacterial infection in higher organisms.
Musthafa
K Syed
KS
Department of Biotechnology, Alagappa University, Karaikudi, India.
Ravi
A Veera
AV
Annapoorani
A
A
Packiavathy
I Sybiya Vasantha
IS
Pandian
S Karutha
SK
D054742:Acyl-Butyrolactones / Q000037:antagonists & inhibitors*; D031230:Ananas; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002364:Caseins / Q000378:metabolism; D002861:Chromobacterium / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D005638:Fruit; D007211:Indoles / Q000378:metabolism; D032129:Manilkara; D008666:Metalloendopeptidases / Q000378:metabolism; D045726:Metalloproteases / Q000378:metabolism; D028521:Musa; D031339:Ocimum; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010945:Plants, Edible; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D011710:Pyocyanine / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects*; D037521:Virulence Factors / Q000378:metabolism
D054742:Acyl-Butyrolactones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002364:Caseins; D007211:Indoles; D010936:Plant Extracts; D037521:Virulence Factors; C026018:azocasein; D011710:Pyocyanine; C548166:LasA protein, Pseudomonas aeruginosa; D045726:Metalloproteases; D010196:Pancreatic Elastase; D008666:Metalloendopeptidases; C066371:staphylolytic protease; C063155:violacein