Gene therapy of salivary diseases.
For many years, our laboratory has been developing gene transfer approaches for salivary gland disorders that currently lack effective therapy. The purpose of this chapter is to describe key methods used in this developmental process. Specifically, we focus on one clinical condition, irradiation-induced salivary hypofunction, and address the choice of transgene and vector to be used, the construction of recombinant viral vectors, how vector delivery is accomplished, and methods for assessing vector function in vitro and in an appropriate animal model.
Baum
Bruce J
BJ
Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
Adriaansen
Janik
J
Cotrim
Ana P
AP
Goldsmith
Corinne M
CM
Perez
Paola
P
Qi
Senrong
S
Rowzee
Anne M
AM
Zheng
Changyu
C
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D012466:Salivary Gland Diseases / Q000235:genetics / Q000628:therapy*