Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation.
Adult stem cells are nowadays used for treating several pathologies. A putative stem cell population was found in the adipose tissue of mammals and canine adipose tissue-derived-mesenchymal stem cells (cA-MSC) have been shown to possess the capacity to differentiate into several lineages. The main goal of our research was to fully characterize cA-MSC and examine the effects of cryopreservation on their stemness features. Each sample of cA-MSC was analyzed immediately and then again after being frozen in liquid nitrogen for one year. After the cryopreservation period cells conserved their fibroblast-like morphology, alkaline phosphatase positivity and CD expression but showed a lower proliferation ratio and a lower telomerase activity in comparison with fresh cells. Finally, the cryopreservation protocol did not change the cA-MSC adipogenic, osteogenic and myogenic differentiative potential. Our data demonstrate that stored cA-MSC might represent a promising type of progenitor cell for autologous cellular-based therapies in veterinary medicine.
Martinello
T
T
Department of Experimental Veterinary Sciences, University of Padova, Italy.
Bronzini
I
I
Department of Experimental Veterinary Sciences, University of Padova, Italy.
Maccatrozzo
L
L
Department of Experimental Veterinary Sciences, University of Padova, Italy.
Mollo
A
A
Department of Clinical Veterinary Sciences, University of Padova, Italy.
Sampaolesi
M
M
Stem Cell Research Institute, University Hospital Gasthuisberg, Leuven, Belgium.
Mascarello
F
F
Department of Experimental Veterinary Sciences, University of Padova, Italy.
Decaminada
M
M
Department of Experimental Veterinary Sciences, University of Padova, Italy.
Patruno
M
M
Department of Experimental Veterinary Sciences, University of Padova, Italy. Electronic address: marco.pat@unipd.it.
D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D018929:Cell Culture Techniques / Q000662:veterinary; D002454:Cell Differentiation; D015925:Cryopreservation / Q000662:veterinary*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D019098:Telomerase / Q000378:metabolism
D000954:Antigens, Surface; D019098:Telomerase; D000469:Alkaline Phosphatase