title: Differential susceptibility of human trophoblastic (BeWo) and uterine cervical (HeLa) cells to Neospora caninum infection.
abstract: Neospora caninum is an apicomplexan parasite, closely related to Toxoplasma gondii, and causes abortion and congenital neosporosis in cattle worldwide. Trophoblast cells act in mechanisms of innate immune defense at the fetal-maternal interface and no data are available about the interaction of Neospora with human trophoblasts. Thus, this study aimed to verify the susceptibility of human trophoblastic (BeWo) compared with uterine cervical (HeLa) cell lines to N. caninum. BeWo and HeLa cells were infected with different parasite:cell ratios of N. caninum tachyzoites and analyzed at different times after infection for cell viability using thiazolyl blue tetrazole and lactate dehydrogenase assays. Both cell lines were also evaluated for cytokine production and parasite infection/replication assays when pre-treated or not with Neospora lysate antigen (NLA) or human recombinant IFN-γ. Cell viability was increased up to 48 h of infection in both types of cells, suggesting that infection could inhibit early cell death and/or induce cell proliferation. Neospora infection induced up-regulation of the macrophage migration inhibitory factor (MIF), mainly in HeLa cells, which was enhanced by cell pre-treatment by NLA or IFN-γ. Conversely, parasite infection induced down-regulation of the transforming growth factor (TGF-β), mostly in BeWo cells, which was decreased with NLA or IFN-γ pre-treatment. HeLa cells were more susceptible to Neospora infection than BeWo cells and IFN-γ pre-treatment resulted in reduced infection indices in both cell lines. Control of parasite growth was mediated by IFN-γ through an indoleamine-2,3-dioxygenase-dependent mechanism in HeLa cells alone. Based on these results, we concluded that BeWo and HeLa cells are readily infected by N. caninum, although presenting differences in susceptibility to infection, cytokine production and cell viability. Thus, these host cells can be considered in comparative approaches to understand strategies used by N. caninum to survive at the maternal-fetal interface.
authors: Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlândia, Av. Pará 1720, 38400-902 Uberlândia, MG, Brazil.
authors: Julianne V
authors: JV
authors: Carvalho
authors: Celene M O S
authors: CM
authors: Alves
authors: Mariana R D
authors: MR
authors: Cardoso
authors: Caroline M
authors: CM
authors: Mota
authors: Bellisa F
authors: BF
authors: Barbosa
authors: Eloísa A V
authors: EA
authors: Ferro
authors: Neide M
authors: NM
authors: Silva
authors: Tiago W P
authors: TW
authors: Mineo
authors: José R
authors: JR
authors: Mineo
authors: Deise A O
authors: DA
authors: Silva
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D002584:Cervix Uteri / Q000276:immunology / Q000469:parasitology*; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D016207:Cytokines / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D006367:HeLa Cells; D006801:Humans; D018688:Neospora / Q000276:immunology / Q000502:physiology*; D014327:Trophoblasts / Q000276:immunology / Q000469:parasitology*
chemical_list: D016207:Cytokines