Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: implication for ecological risk assessment of soybean crops.
Soybean fields provide habitats for many species of amphibians. However, the persistence and health of amphibian populations may be at risk from the increasing use of pesticides and other agricultural chemicals. We examined the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterases (CbEs) in 11 syntopic species of larval anurans. In vitro effects of malaoxon causing 50% BChE inhibition (IC(50)) were also studied. In addition, we calculated a relative risk index (RI) based on the geographic distributions of the anurans, the phenology of soybean cultivation, and basal enzymatic values related to potential pesticide detoxification. Among the 11 species, AChE activity varied from 17.5 ± 1.6 to 68.2 ± 4.7 nmol min(-1) mg(-1) protein (PT). BChE activity also varied significantly, ranging from 3.3 ± 0.4 to 7.5 ± 0.4 nmol min(-1) mg(-1)PT. Both measures of CbE activities varied widely (CbE α-NA: 2.1 ± 0.5-12.4 ± 1.1 nmol min(-1) mg(-1) PT; CbE-4NPV: 21.8 ± 1.8-102.6 ± 7.9 nmol min(-1) mg(-1) PT). We also corroborate that lower BChE activity levels for the tadpoles were associated at minor IC(50) values. The results of this study demonstrate significant variation in enzymatic levels among several tadpole species and intermediate to high RI values for 7 species. Based on these results, it appears that a conversion of native ecosystems to soybean crops may lead to increased ecological risk for anuran amphibians.
Lajmanovich
Rafael C
RC
National Council for Scientific and Technical Research (CONICET), Buenos Aires, Argentina. lajmanovich@hotmail.com
Peltzer
Paola M
PM
Junges
Celina M
CM
Attademo
Andrés M
AM
Sanchez
Laura C
LC
Bassó
Agustín
A
D000110:Acetylcholinesterase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001001:Anura; D001118:Argentina; D002091:Butyrylcholinesterase / Q000378:metabolism; D043182:Carboxylesterase / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D018556:Crops, Agricultural; D017753:Ecosystem; D005843:Geography; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000201:enzymology; D008294:Malathion / Q000031:analogs & derivatives* / Q000633:toxicity; D018570:Risk Assessment; D013025:Glycine max; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
D007306:Insecticides; C100082:malaoxon; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D008294:Malathion