Formation of N-nitrosothiazolidine metabolites in isolated rat liver hepatocytes.
2[ (14)C]N- Nitrosothiazolidine was metabolized in hepatocytes prepared from the liver of a male Sprague-Dawley rat. The metabolites were separated into non-acidic, acidic and aqueous fractions. Radioactive metabolites were found predominantly in the non-acidic fraction, which possessed 98.9% of the total radioactivity. Analysis of the non-acidic fraction with HPLC and gas chromatography-mass spectrometry allowed the identification of two major components in this fraction; They were 2-hydroxythiazolidine and unmetabolized original N-nitrosothiazolidine, which possessed 34.3% and 61.2% of total radioactivity, respectively.
Cragin
D W
DW
Department of Environmental Toxicology, University of California, Davis, CA 95616, USA.
Shibamoto
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