Cell free DNA detected by a novel method in acute ST-elevation myocardial infarction patients.
BACKGROUND
High levels of circulating cell free DNA (CFD) have been associated with poor prognosis in various diseases. Data pertaining to CFD in acute myocardial infarction (MI) are scarce. The available data have been obtained by either electrophoresis or polymerase chain reaction. We evaluated a novel method for the detection of CFD in patients with ST elevation myocardial infarction (STEMI) and examined its correlation with established markers of necrosis and ventricular function.


METHODS
Serum concentrations of CFD, troponin-T and creatine kinase (CK) were measured simultaneously in 16 randomly selected acute STEMI patients upon admission and at three more time points. 47 healthy subjects served as a control group. CFD was quantified by a novel rapid fluorometric assay. Ejection fraction (EF) was assessed by echocardiography.


RESULTS
Peak CFD levels were significantly higher in patients compared with controls (P = 0.001) and correlated with peak levels of CK and troponin-T (R = 0.79, P <0.001); R = 0.65, P = 0.006, respectively). Peak CFD levels tended to be associated with lower EF (P = 0.075).


CONCLUSIONS
With this method, CFD levels correlated with the levels of established markers of myocardial necrosis but not with EF. The kinetic pattern of CFD release after STEMI and its prognostic value require further investigation.
Shimony
Avi
A
Department of Cardiology, Soroka University Medical Center, Ben Gurion University of the Negev, Beer Sheva, Israel. ashimony@bgu.ac.il;
Zahger
Doron
D
Gilutz
Harel
H
Goldstein
Hagit
H
Orlov
Gennady
G
Merkin
Miri
M
Shalev
Aryeh
A
Ilia
Reuben
R
Douvdevani
Amos
A
D003402:Creatine Kinase / Q000097:blood; D004247:DNA / Q000097:blood; D004452:Echocardiography; D004562:Electrocardiography; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology / Q000628:therapy; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009336:Necrosis / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D013318:Stroke Volume; D020107:Troponin T / Q000097:blood
D020107:Troponin T; D004247:DNA; D003402:Creatine Kinase