Use of an antifungal drug, amphotericin B for isolation of thraustochytrids.
The inhibitory effect of amphotericin B (AMPH) on the growth of fungi during the isolation of thraustochytrids was examined. The growth of fungi was significantly inhibited by addition of AMPH, and therefore colonies of thraustochytrids were not overlaid with fungal mycelia, which resulted in increased efficiency of thraustochytrids isolation.
Taoka
Yousuke
Y
Laboratory of Marine Bioscience, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan. yousuketaoka@nissui.co.jp
Nagano
Naoki
N
Okita
Yuji
Y
Izumida
Hitoshi
H
Sugimoto
Shinichi
S
Hayashi
Masahiro
M
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D058009:Stramenopiles / Q000302:isolation & purification* / Q000382:microbiology
D000935:Antifungal Agents; D000666:Amphotericin B