title: A novel electrochemiluminescence sensor based on bis(2,2'-bipyridine)-5-amino-1,10-phenanthroline ruthenium(II) covalently combined with graphite oxide.
abstract: This communication reports a novel electrochemiluminescence (ECL) sensor based on covalently linking bis(2,2'-bipyridine)-5-amino-1,10-phenanthroline ruthenium(II) (Ru(II)-NH2) with graphite oxide (GO) on a glassy carbon electrode. 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride and N-hydroxy-succinimide were applied to activate the carboxyl groups on the GO surface and catalyze the formation of amido link between Ru(II)-NH2 and carboxyl groups on GO. The composite film was characterized using atomic force microscopy, transmission electron microscopy and Fourier transform infrared absorption spectroscopy. Based on ECL experimental results, the composite film modified electrode displayed high electrochemical activity towards the oxidation of 2-(dibutylamino) ethanol (DBAE). Under optimized conditions, the linear response of ECL intensity to DBAE concentration was valid in the range 6.0×10(-7)-2.0×10(-4) mol L(-1) (r2=0.9948) with a detection limit (S/N=3) of 5.0×10(-8) mol L(-1). Furthermore, the ECL sensor presented good characteristics in terms of stability and reproducibility, promising the development of ECL sensors for biologically important compounds.
authors: Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors: Xiao-Mei
authors: XM
authors: Chen
authors: Geng-Huang
authors: GH
authors: Wu
authors: Jin-Mei
authors: JM
authors: Chen
authors: Ya-Qi
authors: YQ
authors: Jiang
authors: Guo-Nan
authors: GN
authors: Chen
authors: Munetaka
authors: M
authors: Oyama
authors: Xi
authors: X
authors: Chen
authors: Xiao-Ru
authors: XR
authors: Wang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D009942:Organometallic Compounds / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; D010087:Oxides; C415768:tris(2,2'-bipyridyl)ruthenium(II); D006108:Graphite