Multiphoton microscopic imaging of normal human rectum tissue.
In this paper, multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation signals, was used to image microstructures of human rectal mucosa and submucosa. The morphology and distribution of the main components in mucosa layer, goblet cells, intestinal glands, and a little collagen fibers have been clearly monitored, and the content and distribution of collagen, elastic fibers, and blood vessels in submucosa layer have also been distinctly obtained. The variation of these components is very relevant to the pathology in gastrointestinal system, especially early rectal cancer. Our results indicate that the MPM technique has the potential application in vivo in the clinical diagnosis and monitoring of early rectal cancer.
Liu
Nenrong
N
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China.
Chen
Gang
G
Chen
Jianxin
J
Yan
Jun
J
Zhuo
Shuangmu
S
Zheng
Liqin
L
Jiang
Xingshan
X
D000328:Adult; D006801:Humans; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D008875:Middle Aged; D012007:Rectum / Q000648:ultrastructure*