title: Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism.
abstract: Recent studies showed that germ-free (GF) mice are resistant to obesity when consuming a high-fat, high-carbohydrate Western diet. However, it remains unclear what mechanisms are involved in the antiobesity phenotype and whether GF mice develop insulin resistance and dyslipidemia with high-fat (HF) feeding. In the present study, we compared the metabolic consequences of HF feeding on GF and conventional (conv) C57BL/6J mice. GF mice consumed fewer calories, excreted more fecal lipids, and weighed significantly less than conv mice. GF/HF animals also showed enhanced insulin sensitivity with improved glucose tolerance, reduced fasting and nonfasting insulinemia, and increased phospho-Akt((Ser-473)) in adipose tissue. In association with enhanced insulin sensitivity, GF/HF mice had reduced plasma TNF-α and total serum amyloid A concentrations. Reduced hypercholesterolemia, a moderate accretion of hepatic cholesterol, and an increase in fecal cholesterol excretion suggest an altered cholesterol metabolism in GF/HF mice. Pronounced nucleus SREBP2 proteins and up-regulation of cholesterol biosynthesis genes indicate that enhanced cholesterol biosynthesis contributed to the cholesterol homeostasis in GF/HF mice. Our results demonstrate that fewer calorie consumption and increased lipid excretion contributed to the obesity-resistant phenotype of GF/HF mice and reveal that insulin sensitivity and cholesterol metabolism are metabolic targets influenced by the gut microbiota.
authors: Institut National de Recherche Agronomique, UMR1319 Micalis, Jouy-en-Josas, France.
authors: Sylvie
authors: S
authors: Rabot
authors: Mathieu
authors: M
authors: Membrez
authors: Aurélia
authors: A
authors: Bruneau
authors: Philippe
authors: P
authors: Gérard
authors: Taoufiq
authors: T
authors: Harach
authors: Mireille
authors: M
authors: Moser
authors: Frederic
authors: F
authors: Raymond
authors: Robert
authors: R
authors: Mansourian
authors: Chieh J
authors: CJ
authors: Chou
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism*; D004041:Dietary Fats / Q000009:adverse effects*; D005856:Germ-Free Life; D005951:Glucose Tolerance Test; D006003:Glycogen / Q000378:metabolism; D007333:Insulin Resistance / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000097:blood / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
chemical_list: D004041:Dietary Fats; D008055:Lipids; D006003:Glycogen; D002784:Cholesterol