Evaluation of glycosyl hydrolases in the secretome of Aspergillus fumigatus and saccharification of alkali-treated rice straw.
A thermotolerant Aspergillus fumigatus strain isolated from composting pile of mixed industrial waste was found to produce a spectrum of cellulase and hemicellulases when cultured on rice straw solidified substrate. The two-dimensional electrophoresis (2DE) resolved the secretome into 57 distinct protein spots. The zymograms developed against 2DE gels identified the presence of three β-glucosidases and five CBHI/EGI isoforms in the secretome. The peptide mass fingerprinting of 17 protein spots by liquid chromatography mass spectrometry characterized the secretome into different glycosyl hydrolase families. The enzyme cocktail produced by A. fumigatus was capable of efficient hydrolysis of alkali pretreated rice straw (at 7% and 10% w/v) resulting in 95% and 91% saccharification, respectively.
Sharma
Manju
M
Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India. manju_scorpian05@yahoo.co.in
Soni
Rohit
R
Nazir
Asiya
A
Oberoi
Harinder Singh
HS
Chadha
Bhupinder Singh
BS
D000468:Alkalies / Q000737:chemistry*; D001232:Aspergillus fumigatus / Q000201:enzymology*; D044602:Cellulases / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D012275:Oryza / Q000737:chemistry*; D020033:Protein Isoforms / Q000378:metabolism
D000468:Alkalies; D020033:Protein Isoforms; D044602:Cellulases; D006026:Glycoside Hydrolases