title: Synthesis, characterization and antibacterial activity of FeIII, CoII, CuII and ZnII complexes probed by transmission electron microscopy.
abstract: We synthesized iron(III), cobalt(II), copper(II) and zinc(II) complexes [Fe(III)(HBPClNOL)Cl(2)]·H(2)O (1), [Co(II)(H(2)BPClNOL)Cl(2)] (2), [Cu(II)(H(2)BPClNOL)Cl]Cl·H(2)O (3), and [Zn(II)(HBPClNOL)Cl] (4), where H(2)BPClNOL is the ligand (N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]propylamine). The complexes obtained were characterized by elemental analysis, IR and UV-visible spectroscopies, electrospray ionization mass spectrometry (ESI-MS), tandem mass spectrometry (MS/MS), and cyclic voltammetry. X-ray diffraction studies were performed for complexes (3) and (4) revealing the presence of mononuclear and dinuclear structures in solid state for (3). However, the zinc complex is mononuclear in solid state. Biological studies of complexes (1)-(4) were carried out in vitro for antimicrobial activity against nine Gram-positive bacteria (Staphylococcus aureus strains RN 6390B, COL, ATCC 25923, Smith Diffuse, Wood 46, enterotoxigenic S. aureus FRI-100 (SEA+), FRI S-6 (SEB+) and SEC FRI-361) and animal strain S. aureus LSA 88 (SEC/SED/TSST-1+). The following sequence of inhibition promoted by the complexes was observed: (4)>(2)>(3)>(1), showing the effect of the metal on the biological activity. To directly observe the morphological changes of the internal structure of bacterial cells after the treatment, transmission electron microscopy (TEM) was employed. For the most active complex [Zn(II)(HBPClNOL)Cl] (4), granulation deposits around the genetic material and internal material leaking were clearly detected.
authors: Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense, 28013-602 Campos dos Goytacazes, RJ, Brazil. chris@pq.cnpq.br
authors: Christiane
authors: C
authors: Fernandes
authors: Adolfo
authors: A
authors: Horn
authors: Olney
authors: O
authors: Vieira-da-Motta
authors: Vagner M
authors: VM
authors: de Assis
authors: Michelle R
authors: MR
authors: Rocha
authors: Luciana da S
authors: Lda S
authors: Mathias
authors: Erika S
authors: ES
authors: Bull
authors: Adailton J
authors: AJ
authors: Bortoluzzi
authors: Erick Vaz
authors: EV
authors: Guimarães
authors: João Carlos Aquino
authors: JC
authors: Almeida
authors: David H
authors: DH
authors: Russell
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D005290:Ferric Compounds; D008024:Ligands; D003035:Cobalt; D003300:Copper; D015032:Zinc