Methanol extract of Ocimum gratissimum protects murine peritoneal macrophages from nicotine toxicity by decreasing free radical generation, lipid and protein damage and enhances antioxidant protection.
In the present study, methanol extract of Ocimum gratissimum Linn (ME-Og) was tested against nicotine-induced murine peritoneal macrophage in vitro. Phytochemical analysis of ME-Og shown high amount of flavonoid and phenolic compound present in it. The cytotoxic effect of ME-Og was studied in murine peritoneal macrophages at different concentrations (0.1 to 100 microg/ml) using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide (MTT) method. To establish the protective role of ME-Og against nicotine toxicity, peritoneal macrophages from mice were treated with nicotine (10 mM), nicotine+ME-Og (1 to 25 microg/ml) for 12h in culture media. The significantly (P< 0.05) increased super oxide anion generation, reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, myeloperoxidase (MPO) activity, lipid peroxidation, protein carbonyls, oxidized glutathione levels were observed in nicotine-treated group as compared to control group; those were significantly (p < 0.05) reduced in ME-Og supplemented groups in concentration dependent manner. More over, significantly (p < 0.05) reduced antioxidant status due to nicotine exposure was effectively ameliorated by ME-Og supplementation in murine peritoneal macrophages. Among the different concentration of ME-Og, maximum protective effect was observed by 25 microg/ml, which does not produce significant cell cytotoxicity in murine peritoneal macrophages. These findings suggest the potential use and beneficial role of O. gratissimum as a modulator of nicotine-induced free radical generation, lipid-protein damage and antioxidant status in important immune cell, peritoneal macrophages.
Mahapatra
Santanu Kar
SK
Immunology and Microbiology Laboratory, Department of Human Physiology, Community Health, Vidyasagar University, Midnapore, West Bengal, India.
Chakraborty
Subhankari Prasad
SP
Das
Subhasis
S
Roy
Somenath
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002470:Cell Survival; D005609:Free Radicals / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D051379:Mice; D009249:NADP / Q000378:metabolism; D009538:Nicotine / Q000633:toxicity*; D031339:Ocimum / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects*
D000975:Antioxidants; D005609:Free Radicals; D010936:Plant Extracts; D009249:NADP; D009538:Nicotine; D009195:Peroxidase; D005978:Glutathione; D000432:Methanol