[Inhibition of genistein on the proliferation of RWPE-1 cells induced by testosterone under the control of insulin-like growth factor-1].
OBJECTIVE
To observe the inhibition of genistein (Gen) on the proliferation of RWPE-1 cells under insulin-like growth factor-1(IGF-1) modulation and the molecular mechanism of influences on cell cycles.


METHODS
The proliferation of RWPE-1 cells was promoted by testosterone and assessed by morphologic observation. RWPE-1 cells were treated with Gen of 0.63 - 80 micromol/L and IGF-1 of 0.1 - 12.8 nmol/L for 24 h, 48 h and 72 h, and the viability of the cell was examined. The cell cycle of RWPE-1 in the control group, 40 micromol/L Gen group, 6.4 nmol/L IGF-1 group and 40 micromol/L Gen + 6.4 nmol/L IGF-1 group was observed by flow cytometry analysis. The expression of cyclin B1, cyclinD1 and cyclin-dependent kinase 4 was examined by Western blot.


RESULTS
The proliferation of RWPE-1 cells could be induced by 2 micromol/L of testosterone, and the proliferation could be inhibited by 2.5 - 80 micromol/L of Gen. The inhibitive effect could be enhanced with increased concentration and prolonged action time. The percentage of cells at G0/G1 phase could be decreased and the percentage of cells both at S phase and G2/M phase could be increased, the expression of cyclinD1 and CDK4 was down-regulated and the expression of cyclinB1 was up-regulated by the treatment of 40 micromol/L Gen under the modulation of 6.4 nmol/L IGF-1.


CONCLUSIONS
Under the modulation of IGF-1, Gen could affect the expression of cyclins and CDKs, induce G2/M cell phase arrested and inhibit the proliferation of RWPE-1 cells induced by testosterone.
Ren
Guofeng
G
Department of Nutrition and Food Hygiene, School of Public Health, Central South University, Changsha 410078, China. rgf3824@163.com
Tang
Ling
L
Zhu
Mingyuan
M
Yang
Li'na
L
Huang
Yiming
Y
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D011467:Prostate / Q000166:cytology; D013739:Testosterone / Q000037:antagonists & inhibitors*
D016213:Cyclins; D013739:Testosterone; D007334:Insulin-Like Growth Factor I; D019833:Genistein; D051358:Cyclin-Dependent Kinase 4