Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients.
In this work, the presence of NS1 protein as a possible early marker of dengue infection was studied in serum samples from confirmed adult patients with a primary and secondary dengue 4 infection. A total of 209 serum samples collected from day 2 up to day 7 of fever onset from 71 patients were tested by Platelia NS1 antigen capture ELISA kit (BioRad, Marnes-la-Coquette, France), and the results were compared with those obtained by capture antidengue virus IgM (MAC)-ELISA and ELISA inhibition method tests. The 83.3% of primary cases and 96.4% of secondary cases were NS1 positive. The kinetics of NS1 protein showed the highest values in optical density mean ratio or in percentage of positives between days 2 and 4. The results obtained in this study show the utility of the NS1 protein as a virologic early marker of dengue infection. Prospective studies should be carried out to confirm its utility as a prognostic marker of severe illness.
Vazquez
Susana
S
Pedro Kourí Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba. svazquez@ipk.sld.cu
Ruiz
Didye
D
Barrero
Rafael
R
Ramirez
Rosa
R
Calzada
Naifi
N
del Rosario Peña
Blanca
B
Reyes
Silvia
S
Guzman
María Guadalupe
MG
D000208:Acute Disease; D000328:Adult; D003715:Dengue / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007700:Kinetics; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017361:Viral Nonstructural Proteins