[Changes of caspase-9 in rat retina caused by Nd:YAG laser injury].
OBJECTIVE
To investigate the changes of caspase-9 in the retina of Long Evans rats caused by Nd: YAG laser injury.


METHODS
A model of retina injury in rats caused by Nd:YAG laser was established. The activity and the expression of mRNA and protein of caspase-9 were detected at Oh, 3h, 6h, 24h, 3d and 7d after laser irradiation. RESULTS; No conspicuous change of caspase-9 mRNA in retina was observed just after laser irradiation, but the activity of caspase-9 and the expression of caspase-9 mRNA and protein increased significantly at 6h and 12h after irradiation (P < 0.05).


CONCLUSIONS
Caspase-9 in the retina of LE rat was activated by Nd:YAG laser irradiation.
Xu
Qing
Q
Department of nutrition, General Hospital of Chinese PLA, Beijing 100853, China. aqing_930@163.com
Wei
Aimin
A
Zhang
Yanwen
Y
Yu
Zhengping
Z
Xue
Changyong
C
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D053844:Lasers, Solid-State / Q000009:adverse effects*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000201:enzymology*; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects*
D012333:RNA, Messenger; C506435:Casp9 protein, rat; D053453:Caspase 9