Photocleavable polyethylene glycol for the light-regulation of protein function.
PEGylation is commonly employed to enhance the pharmacokinetic properties of proteins, but it can interfere with natural protein function. Protein activity can thus be abrogated through PEGylation, and a controllable means to remove the polyethylene glycol (PEG) group from the protein is desirable. As such, light affords a unique control over biomolecules through the application of photosensitive groups. Herein, we report the synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity.
Georgianna
Wesleigh E
WE
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Lusic
Hrvoje
H
McIver
Andrew L
AL
Deiters
Alexander
A
D018360:Crystallography, X-Ray; D008958:Models, Molecular; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D055668:Photochemical Processes; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D011092:Polyethylene Glycols; D009113:Muramidase