title: Immunological fingerprinting of group B streptococci: from circulating human antibodies to protective antigens.
abstract: Group B streptococcus is one of the most important pathogens in neonates, and causes invasive infections in non-pregnant adults with underlying diseases. Applying a genomic approach that relies on human antibodies we identified antigenic GBS proteins, among them most of the previously published protective antigens. In vitro analyses allowed the selection of conserved candidate antigens that were further evaluated in murine lethal sepsis models using several GBS strains. In active and passive immunization models, we identified four protective GBS antigens, FbsA and BibA, as well as two hypothetical proteins, all shown to contribute to virulence based on gene deletion mutants. These protective antigens have the potential to be components of novel vaccines or targets for passive immune prophylaxis against GBS disease.
authors: Intercell AG, Campus Vienna Biocenter 3, 1030 Vienna, Austria.
authors: Andreas L
authors: AL
authors: Meinke
authors: Beatrice M
authors: BM
authors: Senn
authors: Zehra
authors: Z
authors: Visram
authors: Tamás Z
authors: TZ
authors: Henics
authors: Duc Bui
authors: DB
authors: Minh
authors: Wolfgang
authors: W
authors: Schüler
authors: Christina
authors: C
authors: Neubauer
authors: Dieter
authors: D
authors: Gelbmann
authors: Birgit
authors: B
authors: Noiges
authors: Jan
authors: J
authors: Sinzinger
authors: Markus
authors: M
authors: Hanner
authors: Shailesh
authors: S
authors: Dewasthaly
authors: Urban
authors: U
authors: Lundberg
authors: Knut
authors: K
authors: Hordnes
authors: Helga
authors: H
authors: Masoud
authors: Paul
authors: P
authors: Sevelda
authors: Alexander
authors: A
authors: von Gabain
authors: Eszter
authors: E
authors: Nagy
mesh_terms: D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D017353:Gene Deletion; D023281:Genomics; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D009154:Mutation; D011817:Rabbits; D018805:Sepsis / Q000276:immunology / Q000382:microbiology; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; C499709:FbsA protein, Streptococcus agalactiae; C119204:streptococcal protective antigen