title: Transmitted HIV drug resistance among HIV-infected voluntary counseling and testing centers (VCTC) clients in Mumbai, India.
abstract: A survey for transmitted HIV drug resistance (HIVDR) was conducted according to WHO guidelines among clients newly diagnosed with HIV-1 infection at two voluntary counseling and testing centers (VCTC) in Mumbai. HIVDR testing was performed using the ViroSeq RT-PCR method (Abbott). Out of 50 successfully amplified and sequenced specimens, analysis of the first 34 consecutively collected specimens revealed no nucleoside reverse transcriptase inhibitor, nonnucleoside reverse transcriptase inhibitor, or protease inhibitor mutations from the 2007 WHO list of mutations for surveillance of transmitted HIVDR, indicating that the prevalence of transmitted HIVDR to all three drug classes was <5% among recently infected VCTC clients in Mumbai. The phylogenetic analysis revealed that all samples belonged to HIV-1 subtype C. Continued ART program monitoring and further evaluation of transmitted HIV drug resistance in coming years are essential in Mumbai as well as in other regions of the country in which ART is being scaled up rapidly.
authors: National AIDS Research Institute, Bhosari, Pune, India.
authors: Devidas N
authors: DN
authors: Chaturbhuj
authors: Nitin K
authors: NK
authors: Hingankar
authors: Padmini
authors: P
authors: Srikantiah
authors: Renu
authors: R
authors: Garg
authors: Sandhya
authors: S
authors: Kabra
authors: Pravin S
authors: PS
authors: Deshmukh
authors: Sushma D
authors: SD
authors: Jadhav
authors: Smita R
authors: SR
authors: Thorat
authors: Sharda R
authors: SR
authors: Datkar
authors: Preeti
authors: P
authors: Mehta
authors: Nayana
authors: N
authors: Ingole
authors: Meenakshi
authors: M
authors: Mathur
authors: Anita
authors: A
authors: Ramachandran
authors: Partha
authors: P
authors: Haldar
authors: D C S
authors: DC
authors: Reddy
authors: Damodar
authors: D
authors: Bachani
authors: Sujatha
authors: S
authors: Rao
authors: Srikanth P
authors: SP
authors: Tripathy
authors: Ramesh S
authors: RS
authors: Paranjape
mesh_terms: D019943:Amino Acid Substitution; D024921:Drug Resistance, Multiple, Viral / Q000187:drug effects / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D017320:HIV Protease Inhibitors / Q000627:therapeutic use; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D017423:Sequence Analysis, RNA; D055815:Young Adult
chemical_list: D017320:HIV Protease Inhibitors; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors