[Expression of partial S gene of Hantavirus Z10 in Pichia pastoris].
OBJECTIVE
The purpose of this study is to express partial S gene of Hantavirus Z10.


METHODS
The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.


RESULTS
The recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.


CONCLUSIONS
The nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.
Zhu
Han-ping
HP
The Key Lab of HFRS of Zhejiang Province, The Center for Disease Prevention and Control of Zhejiang Province, Hangzhou, China. hanpingzhu@yahoo.com.cn
Yao
Ping-ping
PP
Deng
Xiao-zhao
XZ
Xu
Fang
F
Xie
Rong-hui
RH
Zhu
Zhi-yong
ZY
Zhang
Yun
Y
D015870:Gene Expression; D006476:Orthohantavirus / Q000235:genetics* / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism
D019590:Nucleocapsid Proteins