Cell adhesive peptide screening of the mouse laminin α1 chain G domain.
Cell adhesive peptides have been widely applied for therapeutic drugs, drug delivery systems, and biomaterials. Previously, we identified various cell adhesive sequences in the G domains of four laminin α chains (α2-α5) by the systematic soluble peptide screening. We also identified five cell-binding sequences in the laminin α1 chain G domain using synthetic peptide-polystyrene beads. Here, we re-screened cell adhesive peptides in the laminin α1 chain G domain by the systematic soluble peptides screening. The 110 soluble peptides were evaluated for their cell adhesive activities using human fibrosarcoma HT1080 cells and human dermal fibroblasts. Fourteen peptides were newly identified as a cell adhesive. Additionally, four peptides (AG22: SSFHFDGSGYAM, AG42: TFDLLRNSYGVRK, AG76: HQNQMDYATLQLQ, AG86: LGGLPSHYRARNI) promoted integrin-mediated cell adhesion. Further, neurite outgrowth activity with rat pheochromocytoma PC12 cells was evaluated and two peptides (AG20: SIGLWNYIEREGK, AG26: SPNGLLFYLASNG) were newly identified for neurite outgrowth activity. These results suggested that the systematic soluble peptides screening approach is an accurate and powerful strategy for finding biologically active sequences. The active sequences newly identified here could be involved in the biological functions of this domain. The active peptides are useful for evaluating molecular mechanisms of laminin-receptor interactions and for developing cell adhesive biomaterials.
Hozumi
Kentaro
K
Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
Akizuki
Taneyasu
T
Yamada
Yuji
Y
Hara
Toshihiro
T
Urushibata
Shunsuke
S
Katagiri
Fumihiko
F
Kikkawa
Yamato
Y
Nomizu
Motoyoshi
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002448:Cell Adhesion; D004492:Edetic Acid / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism; D007797:Laminin / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D016501:Neurites / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary / Q000235:genetics; D051381:Rats
D016023:Integrins; D007797:Laminin; D010455:Peptides; C084098:laminin A; D006493:Heparin; D004492:Edetic Acid