title: The impact of silica gel pore and particle sizes on HPLC column efficiency and resolution for an immobilized, cyclodextrin-based, chiral stationary phase.
abstract: In this study, the CD derivative, mono (6(A)-azido-6(A)-deoxy)-per(p-chlorophenylcarbamonylated) β-CD was chemically immobilized onto the surface of an amino-functionalized silica gel with different pore (100, 300, and 500Å) and particle (3, 5, and 10 μm) sizes to obtain novel chiral stationary phases. The impact of pore and particle size on the amount of immobilized Ph-β-CD, column performance, and enantioselectivity was investigated by evaluating the separation of a variety of racemates in both the normal- and the reversed-phase modes. Experimental results revealed that the retention factor and resolution of racemates generally decreased with increasing pore size; the column prepared with the smallest (3 μm) silica gel particle size gave the best column performance and enantioselectivity in both the normal- and the reversed-phase modes.
authors: School of Chemistry and Environment, South China Normal University, Guangzhou, P. R. China.
authors: Qing
authors: Q
authors: Qin
authors: Sheng
authors: S
authors: Zhang
authors: Wei-Guang
authors: WG
authors: Zhang
authors: Zhen-Bin
authors: ZB
authors: Zhang
authors: Ya-Jin
authors: YJ
authors: Xiong
authors: Zi-Yuan
authors: ZY
authors: Guo
authors: Jun
authors: J
authors: Fan
authors: Sheng
authors: S
authors: Run-Zheng
authors: David
authors: D
authors: Finlow
authors: Yong
authors: Y
authors: Yin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D008422:Materials Testing; D015394:Molecular Structure; D010316:Particle Size; D016062:Porosity; D058428:Silica Gel / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D058428:Silica Gel