Plasmodium falciparum Tudor Staphylococcal Nuclease interacting proteins suggest its role in nuclear as well as splicing processes.
Tudor Staphylococcal Nuclease (p100 or SND1), a member of the micronuclease family is a multifunctional protein that plays a key role(s) in transcription and splicing processes in many eukaryotic cells. PfTudor-SN, a Plasmodium homolog of the human p100 protein is a structurally conserved protein; however molecular details of its function are not yet understood. Our previous studies have shown that PfTudor-SN binds RNA and it is possible to selectively inhibit parasite growth by PfTudor-SN specific drugs. In the present study, we identified the molecular interactions between Plasmodium falciparum Tudor-SN and twelve Plasmodium proteins such as Histone h2A, SPT2 (a transcriptional regulator), a Cold-shock DNA binding protein in a bacterial two-hybrid screen. To get further insight into some of these interactions, we mapped the interaction domain in PfTudor-SN protein using the yeast two-hybrid system. Of these proteins, Plasmodium N-methyl-d-aspartate receptor associated protein, PfUbiquitin conjugating enzyme and Cold-shock DNA binding protein showed interaction with the SN domains of PfTudor-SN. Immuno-localization studies of the interacting proteins showed their presence predominantly in the nucleus, which inevitably suggests the molecular interactions between these proteins and PfTudor-SN. Furthermore, we also identified a molecular interaction between the Tudor domain of PfTudor-SN protein and Plasmodium spliceosomal Sm protein, PfSmD1 advocating the role of PfTudor-SN in the spliceosome assembly. Together, these results suggest multiple role(s) for PfTudor-SN protein mainly in nuclear and splicing processes at asexual blood stages of the malaria parasite.
Hossain
Manzar J
MJ
Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi-110067, India.
Korde
Reshma
R
Singh
Prashant K
PK
Kanodia
Shivani
S
Ranjan
Ravi
R
Ram
Geeta
G
Kalsey
Gaganjot Singh
GS
Singh
Rita
R
Malhotra
Pawan
P
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics*; D015723:Gene Library; D047468:Immunoprecipitation; D008018:Life Cycle Stages; D008836:Micrococcal Nuclease / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D012326:RNA Splicing / Q000502:physiology*; D017381:Spliceosomes / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D010455:Peptides; D015800:Protozoan Proteins; D008836:Micrococcal Nuclease