Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs.
Distribution and characterization of interlukin-10 (IL-10)-secreting cells in lymphoid tissues of pigs naturally infected with porcine circovirus type 2 (PCV2) were evaluated in accordance with PCV2 antigen detection. After screening a total of 56 pigs showing the symptoms of postweaning multisystemic wasting syndrome (PMWS), 15 pigs were PCV2 positive and 5 pigs, which showed stronger positive signals over multiples tissues were further investigated. This study showed that in PCV2-infected lymphoid tissues, particularly mandibular lymph node, spleen and tonsil, IL-10 expression was mainly localized in T-cell rich areas but rarely in B cell rich areas. IL-10 was highly expressed in bystander cells but rarely in PCV2-infected cells. Elevated IL-10 expression was predominantly associated with T cells, but rarely with B cells or with macrophages. The results of this study provide evidence for the role of IL-10 in chronic PCV2 infection and its relation to PCV2 antigen in affected tissues. Constantly elevated levels of IL-10 lead to immunosuppression in persistent and chronic viral infections. The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection.
Doster
Alan R
AR
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA.
Subramaniam
Sakthivel
S
Yhee
Ji Young
JY
Kwon
Byung Joon
BJ
Yu
Chi Ho
CH
Kwon
So Young
SY
Osorio
Fernando A
FA
Sur
Jung Hyang
JH
D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D007150:Immunohistochemistry / Q000662:veterinary; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology* / Q000473:pathology; D056910:Republic of Korea; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology
D016753:Interleukin-10