Development of a microwave-accelerated metal-enhanced fluorescence 40 second, <100 cfu/ml point of care assay for the detection of Chlamydia trachomatis.
An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with "bow-tie" structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new "release and detect" platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.
Zhang
Yongxia
Y
Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland Baltimore County, MD 21202, USA. yongxia@umbc.edu
Agreda
Patricia
P
Kelley
Shannon
S
Gaydos
Charlotte
C
Geddes
Chris D
CD
D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008872:Microwaves; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D019095:Point-of-Care Systems; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004269:DNA, Bacterial