Caspase-3 and Bcl-2 expression in glioblastoma: an immunohistochemical study.
The unfavorable prognosis of malignant gliomas can also be explained by the incomplete knowledge of their molecular pathways. Studies regarding the regulatory process of apoptosis in glioblastoma (GBM), the most common malignant glioma, are few, and better knowledge of the expression of pro and anti-apoptotic proteins could collaborate with the development of new treatments founded on molecular basis. The objective of this study was to evaluate by immunohistochemistry the expression of caspase-3 and Bcl-2 in 30 samples of GBMs. The expression of caspase-3 (mean 17.67%) was lower than Bcl-2 (mean 30.92%), a statistically significant result (p<0.0001), suggesting low apoptotic activity in these tumors. Other studies of proteins related to the intrinsic and extrinsic pathway of apoptosis are required to provide additional information of this mechanism in GBMs.
Tirapelli
Luís Fernando
LF
Department of Surgery and Anatomy, Medicine School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. tirapeli@fmrp.usp.br
Bolini
Paulo Henrique Nasser Andre
PH
Tirapelli
Daniela Pretti da Cunha
DP
Peria
Fernanda Maris
FM
Becker
Aline Nazareth Paixão
AN
Saggioro
Fabiano Pinto
FP
Carlotti
Carlos Gilberto
CG
D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D005260:Female; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3