Pentane formation and changes in fatty acid composition accompanying lipid peroxidation induced by carbon tetrachloride in guinea pig liver microsomes.
A microsomal suspension of guinea pig liver was incubated under anaerobic conditions with carbon tetrachloride (CCl(4); final concentration, 2.1 mmol/litre) and NADPH (final concentration, 13.4 mmol/litre). Following incubation, 0.5 ml of the gas phase was analysed by gas chromatography to measure pentane. The amount of thiobarbituric acid (TBA)-reactive substances in the microsomal suspension was also measured and the composition of the fatty acids in the microsomal fraction was determined. Pentane formation increased significantly with incubation time and the dose of CCl(4). TBA-reactive substances showed no significant increase. Linoleic acid and arachidonic acid, which are precursors of pentane, decreased with incubation time and the dose of CCl(4) administered. However, there were no changes in the amounts of palmitic acid, stearic acid or oleic acid. We therefore conclude that pentane measurement is a useful indicator of in vitro lipid peroxidation.
Sato
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N
Department of Anesthesiology and Critical Care Medicine, Hiroshima University, School of Medicine, Kasumi 1-2-3, Minami-Ku, Hiroshima 7324, Japan.
Fujii
K
K
Yuge
O
O