NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
NfrA1 nitroreductase from the Gram-positive bacterium Bacillus subtilis is a member of the NAD(P)H/FMN oxidoreductase family. Here, we investigated the reactivity, the structure and kinetics of NfrA1, which could provide insight into the unclear biological role of this enzyme. We could show that NfrA1 possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide. Finally, we showed that NfrA1 is able to rapidly scavenge H(2)O(2) produced during the oxidative process or added exogenously.
Cortial
Sylvie
S
Institut de Chimie des Substances Naturelles (ICSN), CNRS UPR 2301, Gif-sur-Yvette, France.
Chaignon
Philippe
P
Iorga
Bogdan I
BI
Aymerich
Stéphane
S
Truan
Gilles
G
Gueguen-Chaignon
Virginie
V
Meyer
Philippe
P
Moréra
Solange
S
Ouazzani
Jamal
J
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009536:Niacinamide / Q000096:biosynthesis; D009601:Nitroreductases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D011487:Protein Conformation; D013481:Superoxides / Q000378:metabolism
D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D009243:NAD; D013481:Superoxides; D009536:Niacinamide; D006861:Hydrogen Peroxide; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C116226:NfrA1 protein, Bacillus subtilis