title: Microwave-assisted extraction of human hair proteins.
abstract: The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25 mM Tris-HCl (pH 8.5), 2.6 M thiourea, 5 M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600 W) for a specified incubation period (5-120 min). The extraction efficiency of samples that had been incubated for 60 min was similar to that of samples that had been heated at 50°C for 24 h using the conventional Shindai method.
authors: Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
authors: Seongjae
authors: S
authors: Shin
authors: Aera
authors: A
authors: Lee
authors: Sunyoung
authors: S
authors: Lee
authors: KyungJun
authors: K
authors: Lee
authors: Jongeun
authors: J
authors: Kwon
authors: Mi Young
authors: MY
authors: Yoon
authors: Jangmi
authors: J
authors: Hong
authors: Dongwook
authors: D
authors: Lee
authors: Gae-Ho
authors: GH
authors: Lee
authors: Jeongkwon
authors: J
authors: Kim
mesh_terms: D006197:Hair / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000737:chemistry / Q000302:isolation & purification; D008872:Microwaves; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011506:Proteins; D007633:Keratins