Correlation of oxidative stress and inflammatory markers with the severity of sickle cell nephropathy.
BACKGROUND
Reactive oxygen species have been shown to mediate inflammatory process and may be involved in lipid peroxidation.


METHODS
This study evaluates superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde, C- reactive protein and fibrinogen in the serum of patients with sickle cell disease and their correlation with renal insufficiency. Superoxide dismutase, glutathione peroxides and C - reactive protein were assayed using sandwich ELISA technique while malondialdehyde and fibrinogen were determined using thiobarbituric reactive substance and turbidometric technique, respectively.


RESULTS
The study group consisted of 40 patients with sickle cell disease along with macroalbuminuria, 16 with chronic kidney disease and 144 sickle cell disease controls. Superoxide dismutase, glutathione peroxidase and catalase were decreased while malondialdehyde, C-reactive protein and fibrinogen were increased in patients with sickle cell disease along with renal insufficiency. These parameters correlated with the severity of renal disease.


CONCLUSIONS
Oxidative stress and inflammatory parameters correlate with sickle cell disease nephropathy.
Emokpae
M A
MA
Department of Chemical Pathology, Aminu Kano Teaching Hospital, Kano, Nigeria. biodunemokpae@yahoo.com
Uadia
P O
PO
Gadzama
A A
AA
D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000652:urine; D000755:Anemia, Sickle Cell / Q000097:blood / Q000150:complications*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000209:etiology*; D012720:Severity of Illness Index; D055815:Young Adult
D015415:Biomarkers; D017382:Reactive Oxygen Species