title: Accuracy of transrectal palpation for early pregnancy diagnosis in Egyptian buffaloes.
abstract: The present study was undertaken to evaluate the accuracy of transrectal palpation (TRP) for diagnosing early pregnancy in buffaloes and the false diagnoses of the TRP test by using the pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) test. Pregnancy was diagnosed in 168 buffalo-cows once by TRP and PAG-RIA test between days 31 and 55 after breeding. The sensitivity of TRP for detecting pregnant buffalo-cows was 37.5% at days 31-35, increased to 93.8% at days 46-50 and reached 100% at days 51-55 (P < 0.01). All cases of false negative diagnoses (n = 10) had PAG concentration higher than the threshold (≥1.8 ng/mL) for diagnosing pregnancy. The specificity of TRP for detecting non-pregnant buffalo cows ranged between 90.9%, and 100% between days 31 and 55. All cases of false positive diagnoses (n = 5) made by TRP had PAG concentrations lower than the threshold for diagnosing pregnancy. It could be concluded that TRP is an accurate method for diagnosing pregnant and non-pregnant buffalo cows from day 46 after breeding.
authors: Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt. alykaren@hotmail.com
authors: Aly Mohamed
authors: AM
authors: Karen
authors: Samy
authors: S
authors: Darwish
authors: Adel
authors: A
authors: Ramoun
authors: Khalid
authors: K
authors: Tawfeek
authors: Van Hanh
authors: VH
authors: Nguyen
authors: Noelita Melo
authors: NM
authors: de Sousa
authors: Jose
authors: J
authors: Sulon
authors: Otto
authors: O
authors: Szenci
authors: Jean-Francios
authors: JF
authors: Beckers
mesh_terms: D000367:Age Factors; D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D051517:Digital Rectal Examination / Q000379:methods / Q000662:veterinary*; D005260:Female; D011237:Predictive Value of Tests; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011258:Pregnancy Tests / Q000662:veterinary*; D011863:Radioimmunoassay / Q000662:veterinary; D012680:Sensitivity and Specificity
chemical_list: D011257:Pregnancy Proteins