title: Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production.
abstract: The objective was to evaluate the structural and functional quality of bull sperm after sexing by flow cytometry. Frozen non-sexed (NS), sexed for X (SX) and sexed for Y (SY) sperm from four bulls was used. Frozen-thawed sperm was analyzed for motility, sperm head agglutination, morphology, capacitation, and integrity of the plasma membrane, acrosome, and chromatin. After Percoll centrifugation (45:60% gradients), the pellet was used for sperm analysis or IVF. Data were analyzed using generalized linear models (P < 0.05) and were reported as least squares means ± standard error (SEM). Based on sperm evaluations, NS sperm had better (P < 0.05) quality than sexed sperm, including higher motility and greater percentages of cells with an intact membrane and acrosome (58.0 ± 3.0, 58.2 ± 3.0, and 60.9 ± 3.3) than SX (29.6 ± 1.3, 36.0 ± 2.9, and 37.1 ± 3.3), and SY (26.2 ± 2.1, 36.4 ± 2.9, and 37.5 ± 3.3). There were no differences (P > 0.05) among groups for fertilization and cleavage rates. Similarly, blastocyst rate on Day 8 (Day 0 = day of insemination) did not differ among groups (22.2 ± 3.2, 18.1 ± 3.3, and 14.8 ± 2.9 for NS, SX, and SY, respectively). Regarding embryo development kinetics, all groups had similar developmental stages from Days 6 to 9. Although the sex-sorting procedure affected sperm characteristics, it did not significantly affect fertilization or embryo development.
authors: Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasilia, DF 70770-900, Brazil.
authors: J O
authors: JO
authors: Carvalho
authors: R
authors: R
authors: Sartori
authors: G M
authors: GM
authors: Machado
authors: G B
authors: GB
authors: Mourão
authors: M A N
authors: MA
authors: Dode
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D015925:Cryopreservation / Q000662:veterinary; D047108:Embryonic Development; D005307:Fertilization in Vitro / Q000662:veterinary*; D005434:Flow Cytometry / Q000662:veterinary; D008297:Male; D055101:Semen Analysis / Q000662:veterinary; D012732:Sex Determination Analysis / Q000662:veterinary; D013094:Spermatozoa / Q000166:cytology*