Sodium iodate selectively injuries the posterior pole of the retina in a dose-dependent manner: morphological and electrophysiological study.
Sequential morphological and functional features of retinal damage in mice exposed to different doses (40 vs. 20 mg/kg) of sodium iodate (NaIO(3)) were analyzed. Retinal morphology, apoptosis (TUNEL assay), and function (electroretinography; ERG) were examined at several time points after NaIO(3) administration. The higher dose of NaIO(3) caused progressive degeneration of the whole retinal area and total suppression of scotopic and photopic ERG. In contrast, the lower dose induced much less severe degeneration in peripheral part of retina along with a moderate decline of b- and a-wave amplitudes in ERG, corroborating the presence of regions within retina that retain their function. The peak of photoreceptor apoptosis was found on the 3rd day, but the lower dose induced more intense reaction within the central retina than in its peripheral region. In conclusion, these results indicate that peripheral area of the retina reveals better resistance to NaIO(3) injury than its central part.
Machalińska
Anna
A
Department of Histology and Embryology, Pomeranian Medical University, Szczecin, Poland. annam@sci.pam.szczecin.pl
Lubiński
Wojciech
W
Kłos
Patrycja
P
Kawa
Miłosz
M
Baumert
Bartłomiej
B
Penkala
Krzysztof
K
Grzegrzółka
Ryszard
R
Karczewicz
Danuta
D
Wiszniewska
Barbara
B
Machaliński
Bogusław
B
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004596:Electroretinography; D007452:Iodates / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010786:Photoreceptor Cells / Q000187:drug effects* / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000187:drug effects*; D012160:Retina / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D012162:Retinal Degeneration / Q000139:chemically induced*; D013047:Specific Pathogen-Free Organisms
D007452:Iodates; C032285:sodium iodate