Detection of enzyme activities based on the synthesis of gold nanoparticles in HEPES buffer.
The reduction of tetrachloroaurate ions (AuCl(4)(-)) with 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was used to detect alkaline phosphatase (ALP) activity.
Serizawa
Takeshi
T
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. t-serizawa@bionano.rcast.u-tokyo.ac.jp
Hirai
Yu
Y
Aizawa
Mamoru
M
D000469:Alkaline Phosphatase / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D006046:Gold; D006531:HEPES / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008962:Models, Theoretical
D006046:Gold; D000469:Alkaline Phosphatase; D006531:HEPES