title: Interactions between spider silk and cells--NIH/3T3 fibroblasts seeded on miniature weaving frames.
abstract: BACKGROUND
  Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature.
  
  
  RESULTS
  Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen- and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p<0.01).
  
  
  CONCLUSIONS
  Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties. We could show adhesion mechanics on intracellular level. Additionally, proliferation kinetics were higher than in enzymatically digested controls, indicating that spider silk does not require modification. Recent findings concerning reduction of cell proliferation after exposure could not be met. As biotechnological production of the hierarchical composition of native spider silk fibres is still a challenge, our study has a pioneer role in researching cellular mechanics on native spider silk fibres.
authors: Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany. joernkuhbier@gmx.de
authors: Joern W
authors: JW
authors: Kuhbier
authors: Christina
authors: C
authors: Allmeling
authors: Kerstin
authors: K
authors: Reimers
authors: Anja
authors: A
authors: Hillmer
authors: Cornelia
authors: C
authors: Kasper
authors: Bjoern
authors: B
authors: Menger
authors: Gudrun
authors: G
authors: Brandes
authors: Merlin
authors: M
authors: Guggenheim
authors: Peter M
authors: PM
authors: Vogt
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D047011:Silk / Q000737:chemistry* / Q000494:pharmacology*; D013112:Spiders; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047011:Silk