Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)-cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK-cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
Van Leene
Jelle
J
Department of Plant Systems Biology, VIB, Ghent, Belgium.
Hollunder
Jens
J
Eeckhout
Dominique
D
Persiau
Geert
G
Van De Slijke
Eveline
E
Stals
Hilde
H
Van Isterdael
Gert
G
Verkest
Aurine
A
Neirynck
Sandy
S
Buffel
Yelle
Y
De Bodt
Stefanie
S
Maere
Steven
S
Laukens
Kris
K
Pharazyn
Anne
A
Ferreira
Paulo C G
PC
Eloy
Nubia
N
Renne
Charlotte
C
Meyer
Christian
C
Faure
Jean-Denis
JD
Steinbrenner
Jens
J
Beynon
Jim
J
Larkin
John C
JC
Van de Peer
Yves
Y
Hilson
Pierre
P
Kuiper
Martin
M
De Veylder
Lieven
L
Van Onckelen
Harry
H
Inzé
Dirk
D
Witters
Erwin
E
De Jaeger
Geert
G
D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019295:Computational Biology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004261:DNA Replication; D008156:Luciferases / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015203:Reproducibility of Results
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D046912:Multiprotein Complexes; D008156:Luciferases; D018844:Cyclin-Dependent Kinases