A targeted multilocus genotyping assay for lineage, serogroup, and epidemic clone typing of Listeria monocytogenes.
A 30-probe assay was developed for simultaneous classification of Listeria monocytogenes isolates by lineage (I to IV), major serogroup (4b, 1/2b, 1/2a, and 1/2c), and epidemic clone (EC) type (ECI, ECIa, ECII, and ECIII). The assay was designed to facilitate rapid strain characterization and the integration of subtype data into risk-based inspection programs.
Ward
Todd J
TJ
Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA. todd.ward@ars.usda.gov
Usgaard
Thomas
T
Evans
Peter
P
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D017720:Molecular Epidemiology / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial