Proteomic analysis of Pichindé virus infection identifies differential expression of prothymosin-alpha.
The arenaviruses include a number of important pathogens including Lassa virus and Junin virus. Presently, the only treatment is supportive care and the antiviral Ribavirin. In the event of an epidemic, patient triage may be required to more effectively manage resources; the development of prognostic biomarker signatures, correlating with disease severity, would allow rational triage. Using a pair of arenaviruses, which cause mild or severe disease, we analyzed extracts from infected cells using SELDI mass spectrometry to characterize potential biomarker profiles. EDGE analysis was used to analyze longitudinal expression differences. Extracts from infected guinea pigs revealed protein peaks which could discriminate between mild or severe infection, and between times post-infection. Tandem mass-spectrometry identified several peaks, including the transcriptional regulator prothymosin-alpha. Further investigation revealed differences in secretion of this peptide. These data show proof of concept that proteomic profiling of host markers could be used as prognostic markers of infectious disease.
Bowick
Gavin C
GC
Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
Soman
Kizhake V
KV
Wang
He
H
Aronson
Judith F
JF
Luxon
Bruce A
BA
Lomas
Lee O
LO
Gorenstein
David G
DG
Herzog
Norbert K
NK
D000704:Analysis of Variance; D000818:Animals; D001117:Arenaviridae Infections / Q000378:metabolism* / Q000821:virology; D015415:Biomarkers; D002457:Cell Extracts / Q000737:chemistry; D002460:Cell Line; D004195:Disease Models, Animal; D006168:Guinea Pigs; D051379:Mice; D010537:Peritoneum / Q000166:cytology; D018052:Pichinde virus / Q000502:physiology*; D011498:Protein Precursors / Q000096:biosynthesis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013947:Thymosin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism
D015415:Biomarkers; D002457:Cell Extracts; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin