Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Flap endonuclease 1 (FEN1), a structure-specific endo- and exonuclease, has multiple functions that determine essential biological processes, such as cell proliferation and cell death. As such, the enzyme must be precisely regulated to execute each of its functions with the right timing and in a specific subcellular location. Here we report that FEN1 is methylated at arginine residues, primarily at Arg192. The methylation suppresses FEN1 phosphorylation at Ser187. The methylated form, but not the phosphorylated form, of FEN1 strongly interacts with proliferating cell nuclear antigen (PCNA), ensuring the 'on' and 'off' timing of its reaction. Mutations of FEN1 disrupting arginine methylation and PCNA interaction result in unscheduled phosphorylation and a failure to localize to DNA replication or repair foci. This consequently leads to a defect in Okazaki fragment maturation, a delay in cell cycle progression, impairment of DNA repair and a high frequency of genome-wide mutations.
Guo
Zhigang
Z
Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA.
Zheng
Li
L
Xu
Hong
H
Dai
Huifang
H
Zhou
Mian
M
Pascua
Mary Rose
MR
Chen
Qin M
QM
Shen
Binghui
B
D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D045585:Flap Endonucleases / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008745:Methylation; D010766:Phosphorylation; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011496:Protein Methyltransferases / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases; D015203:Reproducibility of Results
D018809:Proliferating Cell Nuclear Antigen; D001120:Arginine; D006861:Hydrogen Peroxide; D011496:Protein Methyltransferases; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases; D045585:Flap Endonucleases; C468317:FEN1 protein, human