Developmental regulation of glycolysis by 20-hydroxyecdysone and juvenile hormone in fat body tissues of the silkworm, Bombyx mori.
20-Hydroxyecdysone (20E) and juvenile hormone (JH) control a variety of physiological events during insect development and metamorphosis. To understand how 20E and JH developmentally regulate energy metabolism in insects, we performed a genome-wide microarray analysis of fat body tissues isolated from the silkworm, Bombyx mori. Many genes involved in energy metabolism, including genes in the glycolytic pathway, were down-regulated during molting and pupation, when 20E levels are high. Notably, 20E treatment exhibited inhibitory effects on key glycolytic enzyme mRNA levels and activities, and RNA interference of the 20E receptor EcR-USP had the opposite effects to 20E treatment. Meanwhile, JH treatment stimulated both mRNA levels and activities of the key glycolytic enzymes, presumably via antagonizing the 20E action. Taken together, we conclude that 20E acts as a general blocker for glycolysis in the Bombyx fat body during molting and pupation, whereas the physiological role of JH is contrast with 20E during molting.
Tian
Ling
L
Key Laboratory of Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Guo
Enen
E
Wang
Sheng
S
Liu
Shumin
S
Jiang
Rong-Jing
RJ
Cao
Yang
Y
Ling
Erjun
E
Li
Sheng
S
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D004441:Ecdysterone / Q000378:metabolism*; D005216:Fat Body / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006019:Glycolysis; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007605:Juvenile Hormones / Q000378:metabolism*; D018978:Molting
D019476:Insect Proteins; D007605:Juvenile Hormones; D004441:Ecdysterone