title: Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation.
abstract: OBJECTIVE
  Cardiac surgical operation is inseparably linked to the induction of an inflammatory response. Both humoral and cellular regulatory mechanisms are operating to maintain body homeostasis. We followed the changes in the expression of CD200/CD200R regulatory molecules on monocytes and granulocyte of cardiac surgical patients operated on using either standard (OP) or modified "mini-invasive" cardiopulmonary bypass (MOP).
  
  
  METHODS
  Expression of CD200/CD200R regulatory molecules was determined by flow cytometry.
  
  
  RESULTS
  The expression of CD200R on granulocytes was increased after surgery in both groups of patients, but the increase was statistically significant only in OP patients (p<0.01). At this time point, there was a significant difference in CD200R expression on granulocytes when comparing OP to MOP patients, being higher in the former group (p<0.01). The expression of CD200R on monocytes was diminished after surgery and during an early postoperative period in both groups of patients. The expression of CD200 on monocytes was significantly diminished after surgery in both groups (p<0.01). Nonetheless, we observed an increase in CD200 expression in OP patients at the 3rd postoperative day. There was a statistically significantly increased CD200 expression on monocytes of OP patients (p<0.001) at the 3rd postoperative day when we compared OP and MOP groups. The expression of CD200 on granulocytes was significantly higher after surgery and at the 3rd postoperative day in OP when compared to MOP patients.
  
  
  CONCLUSIONS
  CD200R expression on granulocytes was significantly increased, while CD200 and CD200R expression on monocytes was decreased after cardiac surgery.
authors: Department of Clinical Immunology and Allergy, Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Kralove, Czech Republic. krejsek@fnhk.cz
authors: Jan
authors: J
authors: Krejsek
authors: Martina
authors: M
authors: Kolackova
authors: Jiri
authors: J
authors: Mandak
authors: Pavel
authors: P
authors: Kunes
authors: Karolina
authors: K
authors: Jankovicova
authors: Dana
authors: D
authors: Vlaskova
authors: Vladimir
authors: V
authors: Svitek
authors: Ctirad
authors: C
authors: Andrys
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D006348:Cardiac Surgical Procedures / Q000009:adverse effects*; D002315:Cardiopulmonary Bypass; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D064446:Orexin Receptors; D011956:Receptors, Cell Surface / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C477337:CD200R1 protein, human; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; C042739:antigens, CD200