Sequence analysis of meq oncogene among Polish strains of Marek's disease.
The main goal of the present study was to analyse the nucleotide and amino acid sequence of Meq--the main oncoprotein of Marek's disease virus. The conducted analysis of the nucleotide sequences of MDV has shown a significant similarity between Polish and reference strains of MDV assigned to the vvMDV and vv+MDV pathotypes. Some of the detected nucleotide point mutations were specific only for Polish strains. The sequence analysis has shown a number of single substitutions and two poly-amino acid insertions among strains defined as mild-pathogenic (mMDV) and one attenuated vaccine strain CVI988--Rispens. These results indicate that there is a close relation between changes in nucleotide or amino acid sequence of meq and the virulence of Polish MDV strains.
Woźniakowski
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G
National Veterinary Research Institute, Department of Poultry Viral Diseases, Partyzantów 57 Avenue, 24-100 Puławy, Poland. grzegorz.wozniakowski@piwet.pulawy.pl
Samorek-Salamonowicz
E
E
Kozdruń
W
W
D000595:Amino Acid Sequence; D000818:Animals; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000235:genetics*; D008380:Marek Disease / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D011200:Poultry
C100369:Eco-Q protein, Gallid herpesvirus 2; D009856:Oncogene Proteins, Viral