title: A systems approach to analyze transcription factors in mammalian cells.
abstract: Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.
authors: Department of Cell Biology, Erasmus Medical Center, Dr. Molewaterplein 50, Rotterdam, The Netherlands. e.soler@erasmusmc.nl
authors: Eric
authors: E
authors: Soler
authors: Charlotte
authors: C
authors: Andrieu-Soler
authors: Ernie de
authors: Ed
authors: Boer
authors: Jan Christian
authors: JC
authors: Bryne
authors: Supat
authors: S
authors: Thongjuea
authors: Erikjan
authors: E
authors: Rijkers
authors: Jeroen
authors: J
authors: Demmers
authors: Wilfred
authors: W
authors: van IJcken
authors: Frank
authors: F
authors: Grosveld
mesh_terms: D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D049490:Systems Biology / Q000379:methods*; D014157:Transcription Factors / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology
chemical_list: D014157:Transcription Factors