Photo-tunable protein release from biodegradable nanoparticles composed of cinnamic acid derivatives.
A novel functional biodegradable copolymer was prepared by grafting dithiothreitiol (DTT) into poly(3,4-dihydroxycinnamic acid)-co-poly(4-hydroxycinnamic acid) (PCA) by the Michael addition. The PCA-graft-DTT (PCA-DTT) nanoparticles were self-assembled by mixing a DMSO solution of PCA-DTT copolymer and distilled water. The diameter of the PCA-DTT nanoparticles was below 100nm, and increased to about 300nm upon increasing the composition ratio of DTT. The PCA-DTT nanoparticles were crosslinked via [2+2] cyclobutane formation of the cinnamate groups by UV irradiation at λ>280nm. Moreover, a variable size decrement of the nanoparticles after UV crosslinking was observed, depending on the grafting degree of DTT. A model protein, bovine serum albumin (BSA), was successfully encapsulated into the PCA-DTT nanoparticles during the self-assembling process. The protein release behavior was influenced by the grafting degree of DTT and the pH of the buffer. Moreover, the photo-crosslinking of the nanoparticles induced a significant acceleration in the release rate due to shrinkage of the nanoparticles. These biodegradable and photo-responsive nanoparticles possessing photo-tunable release properties would be useful as novel and functional carriers for drug delivery systems.
Shi
Dongjian
D
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan.
Matsusaki
Michiya
M
Akashi
Mitsuru
M
D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D002934:Cinnamates / Q000737:chemistry* / Q000528:radiation effects; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D004229:Dithiothreitol / Q000737:chemistry / Q000528:radiation effects; D004337:Drug Carriers / Q000737:chemistry* / Q000528:radiation effects; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000528:radiation effects; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000528:radiation effects; D013499:Surface Properties; D014466:Ultraviolet Rays
D001672:Biocompatible Materials; D002934:Cinnamates; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D011091:Polyesters; D013438:Sulfhydryl Compounds; C558118:poly(3,4-dihydroxycinnamic acid)-co-poly(4-hydroxycinnamic acid)-graft-dithiothreitiol copolymer; D012710:Serum Albumin, Bovine; D004229:Dithiothreitol