Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-gamma-cyclodextrin.
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method with hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as chiral selector for the enantiomeric separation of econazole is reported. Enantioseparation of econazole was successfully achieved by the optimized CD-MEKC system containing 40mM HP-gamma-CD, 50mM SDS and 20mM phosphate buffer (pH 8) solution with an analysis time of less than 9min. Calibration curves were linear for the two stereoisomers of econazole (r(2)>0.998). Good repeatabilities in the migration time, peak area and peak height were obtained in terms of RSD% ranging from 0.30 to 7.67%. Combination of solid-phase extraction (SPE) procedure using diol column and the CD-MEKC method was successfully applied to the determination of econazole in a formulated cream sample.
Hermawan
Dadan
D
Separation Science and Technology Group (SepSTec), Ibnu Sina Institute for Fundamental Science Studies, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.
Wan Ibrahim
Wan Aini
WA
Sanagi
M Marsin
MM
Aboul-Enein
Hassan Y
HY
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004464:Econazole / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047408:gamma-Cyclodextrins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D047408:gamma-Cyclodextrins; C479659:hydroxypropyl-gamma-cyclodextrin; D004464:Econazole