Functionalization of cyclo-olefin polymer substrates by plasma oxidation: stable film containing carboxylic acid groups for capturing biorecognition elements.
Many current designs in biomedical diagnostics devices are based on the use of low cost, disposable, easy-to-fabricate chips made of plastic material, typically a cyclo-olefin polymer (COP). Low autofluorescence properties of such material, among others, make it ideal substrate for fluorescence-based applications. Functionalization of this plastic substrate for biomolecule attachment is therefore of great importance and the quality of films produced on such surface have often a significant influence on the performance of the device. In this communication we discuss the surface chemistry and some other characteristics of hydrophilic films, containing carboxylic acid functional groups, formed by plasma oxidation of COP and also films containing cross-linked, polymerized acryclic acid produced by sequential deposition of tetraorthosilicate and acrylic acid by plasma enhanced chemical vapor deposition (PECVD). Immobilization of labeled, single stranded DNA revealed high binding capacity for both coatings. To our best knowledge, this is the first example of direct immobilization of biomolecules on just plasma oxidized COP. Furthermore, more sophisticated treatment of the oxidized plastic substrate by PECVD with other organic precursors increased the binding capacity by some 40% than that of just plasma oxidized COP. The carboxy functionalized surfaces, due to the negative charge of the carboxy groups, showed very positive trends towards increasing the signal to noise ratio when charged biomolecules such as DNA, are used.
Gubala
Vladimir
V
Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland. vladimir.gubala@dcu.ie
Le
Nam Cao Hoai
NC
Gandhiraman
Ram Prasad
RP
Coyle
Conor
C
Daniels
Stephen
S
Williams
David E
DE
D000179:Acrylates / Q000097:blood / Q000737:chemistry; D000447:Aldehydes / Q000097:blood / Q000737:chemistry; D015374:Biosensing Techniques; D002264:Carboxylic Acids / Q000097:blood / Q000737:chemistry*; D003516:Cycloparaffins / Q000097:blood / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007659:Ketones / Q000097:blood / Q000737:chemistry; D008567:Membranes, Artificial; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010316:Particle Size; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D017640:Silicates / Q000097:blood / Q000737:chemistry; D013499:Surface Properties
D000179:Acrylates; D000447:Aldehydes; D002264:Carboxylic Acids; D003516:Cycloparaffins; D007659:Ketones; D008567:Membranes, Artificial; D011108:Polymers; D017640:Silicates; D004247:DNA; C036658:acrylic acid