title: Covalent attaching protein to graphene oxide via diimide-activated amidation.
abstract: In this paper, graphene oxide nanosheets (GOS) are functionalized by bovine serum albumin (BSA) via diimide-activated amidation under ambient conditions. The obtained GOS-BSA conjugate is highly water-soluble. Results of atomic force microscopy (AFM), Raman spectra and Fourier transform infrared spectroscopy analysis confirm that GOS-BSA conjugate contains both GOS and BSA protein. AFM image shows that GOS are fully exfoliated. Results of cyclic volatammograms show that the protein in the GOS-BSA conjugate retains its bioactivity. The present method may also provide a way to synthesize graphene-based composites with other biomolecules.
authors: Center of Special Materials and Technology, Fudan University, 220 Handan Road, Shanghai 200433, China.
authors: Jianfeng
authors: J
authors: Shen
authors: Min
authors: M
authors: Shi
authors: Bo
authors: B
authors: Yan
authors: Hongwei
authors: H
authors: Ma
authors: Na
authors: N
authors: Li
authors: Yizhe
authors: Y
authors: Hu
authors: Mingxin
authors: M
authors: Ye
mesh_terms: D000577:Amides / Q000737:chemistry*; D000818:Animals; D002234:Carbodiimides / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D006108:Graphite / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000577:Amides; D002234:Carbodiimides; D010087:Oxides; D014867:Water; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D012710:Serum Albumin, Bovine; D006108:Graphite