Development of genome-specific 5S rDNA markers in Brassica and related species for hybrid testing.
The Brassicaceae are targets for DNA manipulation to modify oil content and composition. However, any strategy for creating novel products using genetic modification or traditional breeding must take into account the potential for hybridization with other Brassica species, many of which are important sources of edible oils. In this study we have tested Brassica carinata, a possible target for oil modification, to establish whether it can cross with other Brassica species and related genera, and we have developed molecular DNA assays to confirm hybridization.
La Mura
Maurizio
M
John Bingham Laboratory, National Institute of Agricultural Botany, Cambridge, UK.
Norris
Carol
C
Sporle
Sue
S
Jayaweera
Dasuni
D
Greenland
Andy
A
Lee
David
D
D001937:Brassica / Q000235:genetics*; D019607:Brassicaceae / Q000235:genetics*; D002678:Chimera / Q000235:genetics*; D003433:Crosses, Genetic; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005819:Genetic Markers / Q000502:physiology; D005820:Genetic Testing / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D031224:Raphanus / Q000235:genetics; D031226:Sinapis / Q000235:genetics; D013045:Species Specificity
D004275:DNA, Ribosomal; D005819:Genetic Markers; D012341:RNA, Ribosomal, 5S