title: Synthesis of linear polyamines with different amine spacings and their ability to form dsDNA/siRNA complexes suitable for transfection.
abstract: In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to phosphorus (N/P) ratios using small-angle neutron scattering (SANS) and atomic force microscopy (AFM) techniques. AFM images showed that while linear poly(ethylene imine) (PEI)/DNA complex results in bigger spherical aggregates, poly(propylene imine)s forms torroid and cigar shaped structures upon complexation with DNA. The poly(butylene imine)s (LPBI)s form compact and soluble DNA complexes with a radii range of R(g) = 15-30 nm. Among the studied linear polyamines, the LPBIs did show the best transfection efficiency.
authors: Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors: Wiebke
authors: W
authors: Fischer
authors: Blandine
authors: B
authors: Brissault
authors: Sylvain
authors: S
authors: Prévost
authors: Marta
authors: M
authors: Kopaczynska
authors: Ioanna
authors: I
authors: Andreou
authors: Andrea
authors: A
authors: Janosch
authors: Michael
authors: M
authors: Gradzielski
authors: Rainer
authors: R
authors: Haag
mesh_terms: D004247:DNA / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D033363:Neutron Diffraction; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D034741:RNA, Small Interfering / Q000737:chemistry*; D053838:Scattering, Small Angle; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods*
chemical_list: D046911:Macromolecular Substances; D011073:Polyamines; D034741:RNA, Small Interfering; D004247:DNA