Determination of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry.
A sensitive and rapid method was developed for quantification of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS). An aliquot of 1 mL plasma sample was extracted with ethyl acetate-dichloromethane. Separation of olprinone and the milrinone (internal standard, IS) from the interferences was achieved on a C(18) column followed by MS/MS detection. The analytes were monitored in the positive ionization mode. Multiple reaction monitoring using the transition of m/z 251 → m/z 155 and m/z 212 → m/z 140 was performed to quantify olprinone and IS, respectively. The method had a total chromatographic run time of 3 min and linear calibration curves over the concentration range of 0.5-60 ng/mL. The lower limit of quantification (LLOQ) was 0.5 ng/mL. The intra- and inter-day precisions were less than 16.3% for low QC level, and 7.1% for other QC levels, respectively. The intra- and inter-day relative errors were ranged between -12.2% and 3.7% for three QC concentration levels. The validated method was successfully applied to the quantification of olprinone concentration in human plasma after intravenous (i.v.) administration of olprinone at a constant rate of infusion of 2 μg/(kg min) for 5 min in order to evaluate the pharmacokinetics.
Zhu
Ping
P
Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 Dongchuan Road, Guangzhou 510080, People's Republic of China.
Wen
Yu-Guan
YG
Chen
Ji-Mei
JM
Zhuang
Jian
J
Zhou
Zhi-Ling
ZL
Zheng
Shao-Yi
SY
Wu
Ruo-Bin
RB
Xiao
Xue-Jun
XJ
Lu
Cong
C
Fan
Rui-Xin
RX
Guo
Hui-Ming
HM
Fan
Xiao-Ping
XP
D000085:Acetates / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics*; D007477:Ions; D008752:Methylene Chloride / Q000737:chemistry; D058987:Phosphodiesterase 3 Inhibitors / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics*; D011728:Pyridones / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics*; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000085:Acetates; D007093:Imidazoles; D007477:Ions; D058987:Phosphodiesterase 3 Inhibitors; D011728:Pyridones; C059498:olprinone; D008752:Methylene Chloride; C007650:ethyl acetate