title: Rat adipose tissue-derived stem cells transplantation attenuates cardiac dysfunction post infarction and biopolymers enhance cell retention.
abstract: BACKGROUND
  Cardiac cell transplantation is compromised by low cell retention and poor graft viability. Here, the effects of co-injecting adipose tissue-derived stem cells (ASCs) with biopolymers on cell cardiac retention, ventricular morphometry and performance were evaluated in a rat model of myocardial infarction (MI).
  
  
  RESULTS
  99mTc-labeled ASCs (1x10(6) cells) isolated from isogenic Lewis rats were injected 24 hours post-MI using fibrin a, collagen (ASC/C), or culture medium (ASC/M) as vehicle, and cell body distribution was assessed 24 hours later by gamma-emission counting of harvested organs. ASC/F and ASC/C groups retained significantly more cells in the myocardium than ASC/M (13.8+/-2.0 and 26.8+/-2.4% vs. 4.8+/-0.7%, respectively). Then, morphometric and direct cardiac functional parameters were evaluated 4 weeks post-MI cell injection. Left ventricle (LV) perimeter and percentage of interstitial collagen in the spare myocardium were significantly attenuated in all ASC-treated groups compared to the non-treated (NT) and control groups (culture medium, fibrin, or collagen alone). Direct hemodynamic assessment under pharmacological stress showed that stroke volume (SV) and left ventricle end-diastolic pressure were preserved in ASC-treated groups regardless of the vehicle used to deliver ASCs. Stroke work (SW), a global index of cardiac function, improved in ASC/M while it normalized when biopolymers were co-injected with ASCs. A positive correlation was observed between cardiac ASCs retention and preservation of SV and improvement in SW post-MI under hemodynamic stress.
  
  
  CONCLUSIONS
  We provided direct evidence that intramyocardial injection of ASCs mitigates the negative cardiac remodeling and preserves ventricular function post-MI in rats and these beneficial effects can be further enhanced by administering co-injection of ASCs with biopolymers.
authors: Heart Institute, University of São Paulo Medical School, São Paulo, Brazil.
authors: Maria E
authors: ME
authors: Danoviz
authors: Juliana S
authors: JS
authors: Nakamuta
authors: Fabio L N
authors: FL
authors: Marques
authors: Leonardo
authors: L
authors: dos Santos
authors: Erica C
authors: EC
authors: Alvarenga
authors: Alexandra A
authors: AA
authors: dos Santos
authors: Ednei L
authors: EL
authors: Antonio
authors: Isolmar T
authors: IT
authors: Schettert
authors: Paulo J
authors: PJ
authors: Tucci
authors: Jose E
authors: JE
authors: Krieger
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001704:Biopolymers / Q000008:administration & dosage / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005337:Fibrin / Q000008:administration & dosage / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D007267:Injections; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001704:Biopolymers; D005337:Fibrin; D003094:Collagen