title: Analysis of horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types.
abstract: Myostatin (MSTN) is a negative modulator of muscle mass. We characterized the horse (Equus caballus) MSTN gene and identified and analysed single nucleotide polymorphisms (SNPs) in breeds of different morphological types. Sequencing of coding, untranslated, intronic, and regulatory regions of MSTN gene in 12 horses from 10 breeds revealed seven SNPs: two in the promoter, four in intron 1, and one in intron 2. The SNPs of the promoter (GQ183900:g.26T>C and GQ183900:g.156T>C, the latter located within a conserved TATA-box like motif) were screened in 396 horses from 16 breeds. The g.26C and the g.156C alleles presented higher frequency in heavy (brachymorphic type) than in light breeds (dolichomorphic type such as Italian Trotter breed). The significant difference of allele frequencies for the SNPs at the promoter and analysis of molecular variance (AMOVA) on haplotypes indicates that these polymorphisms could be associated with variability of morphology traits in horse breeds.
authors: DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, 42123 Reggio Emilia, Italy.
authors: Stefania
authors: S
authors: Dall'Olio
authors: Luca
authors: L
authors: Fontanesi
authors: Leonardo
authors: L
authors: Nanni Costa
authors: Marco
authors: M
authors: Tassinari
authors: Laura
authors: L
authors: Minieri
authors: Adalberto
authors: A
authors: Falaschini
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D006239:Haplotypes; D006736:Horses / Q000033:anatomy & histology / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D055435:Myostatin / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D013008:Somatotypes; D013045:Species Specificity
chemical_list: D055435:Myostatin