Discrimination between receptor- and store-operated Ca(2+) influx in human neutrophils.
Ca(2+) and Sr(2+) entry pathways activated by pro-inflammatory agonists FMLP, LTB(4) and PAF have been compared to thapsigargin in human neutrophils. 2-APB (10microM) increased Ca(2+) influx and to a greater extent in agonist than in thapsigargin stimulated neutrophils. This action of 2-APB was specific to Ca(2+) because 2-APB did not augment Sr(2+) entry in agonist and thapsigargin stimulated neutrophils. This suggests that Ca(2+) and Sr(2+) entry can be used to discriminate between receptor and non-receptor (store)-operated Ca(2+) influx. Our data show for the first time that Pyr3 whilst partially inhibiting agonist induced Ca(2+) influx almost completely abolished Ca(2+) influx after thapsigargin stimulation.
Salmon
Michael D
MD
Leukocyte and Ion Channel Research Laboratory, School of Health and Biosciences, University of East London, Stratford Campus, Romford Road, London E15 4LZ, UK.
Ahluwalia
Jatinder
J
D001896:Boron Compounds / Q000494:pharmacology; D002118:Calcium / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007975:Leukotriene B4 / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000276:immunology; D044169:Receptors, Calcium-Sensing / Q000276:immunology* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013324:Strontium / Q000276:immunology / Q000378:metabolism
D001896:Boron Compounds; D007155:Immunologic Factors; D010972:Platelet Activating Factor; D044169:Receptors, Calcium-Sensing; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; C042932:formylmethionyl-leucyl-phenylalanine methyl ester; C109986:2-aminoethoxydiphenyl borate; D002118:Calcium; D013324:Strontium