High-throughput analysis of hexosamine using a colorimetric method.
A 96-well plate method was developed for analysis of total hexosamine content in biological samples. Four hexosamine monomer derivatives-glucosamine hydrochloride, glucosamine sulfate, galactosamine hydrochloride, and mannosamine hydrochloride-were examined for the linearity of their spectra in the concentration range specified in the assay. The hexosamine concentration analysis range was linear from 0.1 to 1 mM. The quantification of hexosamines from chitin and chitosan upon acid hydrolysis was also tested. Accurate quantification of glucosamine content in chitin and chitosan with different molecular sizes and degrees of acetylation was demonstrated using the new method.
Cheng
Yu-Shen
YS
Department of Biological and Agricultural Engineering, University of California, Davis, 95616, USA.
Labavitch
John
J
VanderGheynst
Jean S
JS
D002686:Chitin / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006595:Hexosamines / Q000032:analysis*; D006868:Hydrolysis; D013464:Sulfuric Acids / Q000737:chemistry
D006595:Hexosamines; D013464:Sulfuric Acids; D002686:Chitin; C008322:mannosamine; D005688:Galactosamine; D048271:Chitosan; D005944:Glucosamine; C033158:sulfuric acid