The recovery and persistence of salivary DNA on human skin.
Salivary DNA is encountered in many crimes, such as sexual assaults and murders. In this study, saliva from three male donors was deposited on the skin of three female recipients. The amount of male salivary DNA remaining on the female skin was measured over a 96-h period using the Quantifiler™ Y Human Male DNA Quantification Kit. In eight of the nine experiments, a full male DNA profile matching the donor was obtained even after 96 h. In addition, the study showed that the concentration of salivary DNA varied from donor to donor and from day to day. The efficiency of two recovery methods, wet and dry swabbing and minitaping, was compared. The results indicate the tapelift method gave higher DNA recovery. This study also examined the secondary transfer of salivary DNA from skin to fabrics. Cotton and polyester give higher DNA transfer than leather.
Kenna
June
J
Forensic Science Laboratory, Garda HQ, Phoenix Park, Dublin 8, Dublin, Ireland.
Smyth
Maureen
M
McKenna
Louise
L
Dockery
Clare
C
McDermott
Seán D
SD
D000681:Amylases / Q000032:analysis; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*; D012867:Skin / Q000737:chemistry*; D013784:Textiles; D013997:Time Factors
D004247:DNA; D000681:Amylases