title: Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with hydrosheets composed of chitin/chitosan, fucoidan, and alginate as wound dressings.
abstract: To create a moist environment for rapid wound healing, a hydrosheet composed of alginate, chitin/chitosan, and fucoidan (ACF-HS) has been developed as a functional wound dressing. The aim of this study was to evaluate the accelerating effect of ACF-HS on wound healing for rat mitomycin C-treated healing-impaired wounds. Full-thickness skin defects were made on the back of rats and mitomycin C was applied onto the wound for 10 minutes to prepare a healing-impaired wound. After thoroughly washing out the mitomycin C, ACF-HS was applied to the healing-impaired wounds. The rats were later euthanized and histological sections of the wounds were prepared. The histological examinations showed significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ACF-HS on days 7 and 14, in comparison with that in alginate fiber (Kaltostat), hydrogel wound dressing (DuoACTIVE), and nontreatment (negative control). Furthermore, in cell culture studies, ACF-HS-absorbed serum and fibroblast growth factor-2 was found to be proliferative for fibroblasts and endothelial cells, respectively, and ACF-HS-absorbed serum was found to be chemoattractive for fibroblasts. However, our results may not be strictly comparable with general healing-impaired wound models in humans because of the cell damage by mitomycin C. In addition, more biocompatibility studies of fucoidan are essential due to the possibility of renal toxicity.
authors: Department of Oral and Maxillofacial Surgery Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan. dr22056@ndmc.ac.jp
authors: Kaoru
authors: K
authors: Murakami
authors: Masayuki
authors: M
authors: Ishihara
authors: Hiroshi
authors: H
authors: Aoki
authors: Shingo
authors: S
authors: Nakamura
authors: Shin-Ichiro
authors: S
authors: Nakamura
authors: Satoshi
authors: S
authors: Yanagibayashi
authors: Megumi
authors: M
authors: Takikawa
authors: Satoko
authors: S
authors: Kishimoto
authors: Hidetaka
authors: H
authors: Yokoe
authors: Tomoharu
authors: T
authors: Kiyosawa
authors: Yasunori
authors: Y
authors: Sato
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology; D002478:Cells, Cultured; D002686:Chitin / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D020405:Dermis / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology; D013463:Sulfuric Acid Esters; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000473:pathology / Q000517:prevention & control; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D019384:Nucleic Acid Synthesis Inhibitors; D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D002686:Chitin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016685:Mitomycin; D020723:Glucuronic Acid; D048271:Chitosan; C007789:fucoidan