In vitro development of primordial follicles after long-term culture of goat ovarian tissue.
This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of the medium every 2 days). Non-cultured (control) and cultured ovarian fragments were processed for histological and ultrastructural analysis. After 28 days of culture, the media supplemented with FSH-2d was the most effective in maintaining the percentage of normal follicles and in promoting follicular growth. Furthermore, both treatments with FSH increased the percentage of the primary follicles. However, ultrastructural studies did not confirm follicular integrity from 14 days of culture onward. In conclusion, culturing tissue for up to 7 days in medium containing FSH alone or combined with FGF-2 maintains caprine preantral follicle integrity and promotes their growth in vitro.
Matos
M H T
MH
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil. htmatos@yahoo.com
Bruno
J B
JB
Rocha
R M P
RM
Lima-Verde
I B
IB
Santos
K D B
KD
Saraiva
M V A
MV
Silva
J R V
JR
Martins
F S
FS
Chaves
R N
RN
Báo
S N
SN
Figueiredo
J R
JR
D000818:Animals; D003470:Culture Media; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006041:Goats / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000648:ultrastructure; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000662:veterinary
D003470:Culture Media; D016222:Fibroblast Growth Factor 2; D005640:Follicle Stimulating Hormone