Ion exchange chromatographic conditions for obtaining individual subunits of soybean beta-conglycinin.
Soybean beta-conglycinin is a complex protein possessing health-promoting properties. beta-Conglycinin is a trimeric glycoprotein. Little information related to methods for separation of the individual chains forming beta-conglycinin has been so far published and it is of great interest. As a consequence, less data on the bioactivities of alpha, alpha' and beta subunits of this glycoprotein have been published. The present research aimed to find out new alternative chromatographic conditions to obtain beta-conglycinin subunits that are free of contaminating proteins. In the present short communication, we propose the use of a two-step ion exchange chromatographic protocol to achieve this goal. Firstly, beta subunit was separated by means of anionic exchange fast protein liquid chromatography. Secondly, alpha and alpha' chains were separated from each other by cationic exchange. Our data indicated the feasibility of proposed fractionation protocol to separate soybean beta-conglycinin alpha and alpha' subunits from other contaminating proteins and to obtain enough amounts of the three individual chains forming this glycoprotein for further characterization and application. The procedure may be easily up-scaled.
Amigo-Benavent
Miryam
M
Food Analysis and Bioactivity Department, Instituto de Investigación en Ciencias de Alimentación, CSIC-UAM, Nicolas Cabrera 9, Campus de la Universidad Autonoma de Madrid, 28049 Madrid, Spain. mamigo@ifi.csic.es
Athanasopoulos
Vasileios I
VI
del Castillo
M Dolores
MD
D052179:Antigens, Plant / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000737:chemistry / Q000302:isolation & purification*; D021122:Protein Subunits / Q000302:isolation & purification; D055314:Seed Storage Proteins / Q000737:chemistry / Q000302:isolation & purification*; D030262:Soybean Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013025:Glycine max / Q000737:chemistry*
D052179:Antigens, Plant; D005916:Globulins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max