title: Chiral separation of econazole using micellar electrokinetic chromatography with hydroxypropyl-gamma-cyclodextrin.
abstract: A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method with hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as chiral selector for the enantiomeric separation of econazole is reported. Enantioseparation of econazole was successfully achieved by the optimized CD-MEKC system containing 40mM HP-gamma-CD, 50mM SDS and 20mM phosphate buffer (pH 8) solution with an analysis time of less than 9min. Calibration curves were linear for the two stereoisomers of econazole (r(2)>0.998). Good repeatabilities in the migration time, peak area and peak height were obtained in terms of RSD% ranging from 0.30 to 7.67%. Combination of solid-phase extraction (SPE) procedure using diol column and the CD-MEKC method was successfully applied to the determination of econazole in a formulated cream sample.
authors: Separation Science and Technology Group (SepSTec), Ibnu Sina Institute for Fundamental Science Studies, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Johor, Malaysia.
authors: Dadan
authors: D
authors: Hermawan
authors: Wan Aini
authors: WA
authors: Wan Ibrahim
authors: M Marsin
authors: MM
authors: Sanagi
authors: Hassan Y
authors: HY
authors: Aboul-Enein
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004464:Econazole / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013237:Stereoisomerism; D047408:gamma-Cyclodextrins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D047408:gamma-Cyclodextrins; C479659:hydroxypropyl-gamma-cyclodextrin; D004464:Econazole