Development of an animal model for wound healing in chronic rhinosinusitis.
OBJECTIVE
To develop a model for sinonasal wounding and evaluation during healing in mice with chronic eosinophilic inflammation.


METHODS
Exploratory controlled study in which chronic eosinophilic nasal and sinus inflammation was established in mice followed by wounding of the sinonasal cavity. Histologic features and gene expression were then studied.


METHODS
University of Utah Center for Comparative Medicine.


METHODS
Chronic eosinophilic inflammation was established in mice. They were then wounded and humanely killed at days 3, 7, and 14 after wounding.


METHODS
Inflammation was assayed by light microscopic examination. Polymerase chain reaction analysis of transforming growth factor-beta1b, insulinlike growth factor (IGF)-1, matrix metalloproteinase (MMP)-7, MMP-9, tissue inhibitor of metalloproteinase 1 (TIMP-1), and prostaglandin E receptor EP4 expression was performed as well. Uninflamed mice were wounded and examined using the same protocol.


RESULTS
Chronically inflamed mice showed higher histologic inflammatory scores before and after wounding. Expression of IGF-1, TIMP-1, and MMP-9 was also higher prior to wounding and during healing. Continued stimulation appears necessary for the chronic eosinophilic inflammation to persist.


CONCLUSIONS
We successfully constructed a model in which wound healing in a setting of chronic eosinophilic inflammation can be studied. In this exploratory pilot, we demonstrated the feasibility of reproducibly wounding the sinonasal cavity of chronically inflamed mice and examining histologic and gene expression effects of the inflammatory response after wounding.
Tansavatdi
Kristina P
KP
Division of Otolaryngology-Head and Neck Surgery, University of Utah School of Medicine, 50 N Medical Dr, Room 3C120, Salt Lake City, UT 84132, USA. Kristina.tansavatdi@hsc.utah.edu
McGill
Lawrence
L
Riggs
Sterling
S
Orlandi
Richard R
RR
D000818:Animals; D001232:Aspergillus fumigatus; D002908:Chronic Disease; D004195:Disease Models, Animal; D004802:Eosinophilia / Q000235:genetics / Q000473:pathology*; D005240:Feasibility Studies; D005260:Female; D015870:Gene Expression / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D020783:Matrix Metalloproteinase 7 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D015523:Maxillary Sinusitis / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D012220:Rhinitis / Q000235:genetics / Q000473:pathology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
C508961:Tgfb1 protein, mouse; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; D007334:Insulin-Like Growth Factor I; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse