Fluid-percussion-induced traumatic brain injury model in rats.
Traumatic brain injury (TBI) is a major cause of mortality and morbidity. Various attempts have been made to replicate clinical TBI using animal models. The fluid-percussion model (FP) is one of the oldest and most commonly used models of experimentally induced TBI. Both central (CFP) and lateral (LFP) variations of the model have been used. Developed initially for use in larger species, the standard FP device was adapted more than 20 years ago to induce consistent degrees of brain injury in rodents. Recently, we developed a microprocessor-controlled, pneumatically driven instrument, micro-FP (MFP), to address operational concerns associated with the use of the standard FP device in rodents. We have characterized the MFP model with regard to injury severity according to behavioral and histological outcomes. In this protocol, we review the FP models and detail surgical procedures for LFP. The surgery involves tracheal intubation, craniotomy and fixation of Luer fittings, and induction of injury. The surgical procedure can be performed within 45-50 min.
Kabadi
Shruti V
SV
Department of Anesthesiology and the Center for Shock, Trauma and Anesthesiology Research (STAR), School of Medicine, Baltimore, Maryland, USA.
Hilton
Genell D
GD
Stoica
Bogdan A
BA
Zapple
David N
DN
Faden
Alan I
AI
D000818:Animals; D001930:Brain Injuries; D004195:Disease Models, Animal; D004867:Equipment Design; D004864:Equipment and Supplies; D008838:Microcomputers; D051381:Rats; D017207:Rats, Sprague-Dawley