Improvement of hydrophobic integral membrane protein identification by mild performic acid oxidation-assisted digestion.
Integral membrane proteins (IMPs) are critical for the maintenance of biological systems and represent important targets for the treatment of disease. The hydrophobicity and low abundance of IMPs make them difficult to analyze. In proteomic analyses, hydrophobic peptides including transmembrane domains are often underrepresented, and this reduces the sequence coverage and reliability of the identified IMPs. Here we report a new strategy, mild performic acid oxidation treatment (mPAOT), for improvement of IMP identification. In the mPAOT strategy, the hydrophobicity of IMPs is significantly decreased by oxidizing their methionine and cysteine residues with performic acid, thereby improving the solubility and enzymolysis of these proteins. The application of the mPAOT strategy to the analysis of IMPs from human nasopharyngeal carcinoma CNE1 cell line demonstrated that many IMPs, including those with high hydrophobicity, could be reliably identified.
Cao
Rui
R
Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha 410081, People's Republic of China.
Liu
Yisong
Y
Chen
Ping
P
Lv
Rong
R
Song
Qin
Q
Sheng
Tingting
T
He
Quanyuan
Q
Wang
Yin
Y
Wang
Xianchun
X
Liang
Songping
S
D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry; D005561:Formates / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry
D005561:Formates; D008565:Membrane Proteins; D010455:Peptides; C008817:peroxyformic acid; D008715:Methionine; D003545:Cysteine