Evaluation of the combined toxic effects of cocaine and ethanol on primary myocardial cell cultures.
Since 1978, the most prevalent drug used in conjunction with cocaine in cocaine-associated myocardial deaths is ethanol. Primary myocardial cell cultures were used to evaluate the acute additive cardiotoxic effects of cocaine and ethanol. The cultures were exposed to 1 x 10(-3)m or 1 x 10(-5)m-cocaine or combinations of both concentrations of cocaine with 600 mg ethanol/100 ml. Alterations in beating activity, morphology, and lactate dehydrogenase (LDH) release were evaluated after 1, 4 and 24 hr of treatment. Although cells exposed to 1 x 10(-5)m-cocaine or to 1 x 10(-5)m-cocaine and ethanol were able to retain some beating activity, no beating activity occurred in cells exposed to 1 x 10(-3)m-cocaine with or without ethanol. Morphologically, pseudopodia and disruption of the monolayer were more extensive in cells treated for 4 or 24 hr with the combinations of cocaine and ethanol in comparison with those treated with cocaine alone. After 24 hr, LDH release in cells exposed to 1 x 10(-3)m-cocaine or 1 x 10(-3)m-cocaine with ethanol was elevated over the level in untreated controls. These data suggest that ethanol enhances cocaine-induced beating abnormalities and morphological alterations in primary myocardial cell cultures.
Welder
A A
AA
College of Pharmacy, The University of Oklahoma Health Sciences Center, Division of Medicinal Chemistry and Pharmacodynamics, 1110 N. Stonewall, Oklahoma City, OK 73190, USA.
O'Dell
J F
JF
Melchert
R B
RB
Eselin
J A
JA