DNAzyme as an efficient tool to modulate invasiveness of human carcinoma cells.
In this study we evaluated efficiency of DNAzymes to modulate motility of cancer cells, an important factor in the progression and metastasis of cancers. For this purpose we targeted β1 integrins that are predominant adhesive receptors in various carcinoma cell lines (CX1.1, HT29, LOVO, LS180, PC-3). To evaluate invasiveness of cancer cells, we used a transwell migration assay that allowed analyzing chemotactic migration of colon carcinoma cell lines across an ECM-coated membrane. Their adhesive properties were also characterized by the analysis of adhesion to fibronectin, laminin and collagen. In addition, the expression of major integrin subunits, selected intact β1 integrins, and other adhesive receptors (ICAM, E-selectin, uPAR) was analyzed by flow cytometry. Inhibition of β1 integrin expression by DNAzyme to β1 mRNA almost abolished the invasiveness of the CX1.1, HT29, LS180, LOVO and PC-3 cells in vitro. These data show that DNAzymes to β1 integrin subunit can be used to inhibit invasiveness of carcinoma cells.
Wiktorska
Magdalena
M
Department of Molecular and Medical Biophysics, Medical University of Łódź, Poland.
Papiewska-Pająk
Izabela
I
Okruszek
Andrzej
A
Sacewicz-Hofman
Izabela
I
Niewiarowska
Jolanta
J
D015153:Blotting, Western; D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D019040:E-Selectin / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism
D021881:DNA, Catalytic; D019040:E-Selectin; D005353:Fibronectins; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D003094:Collagen