Effects of retinoic acid, auranofin and mercuric chloride on plasminogen activator activity in post-implantation cultured mouse embryos.
Plasminogen activator (PA) activity has been suggested to be an important determinant of cell migration and tissue modelling during organogenesis. We have postulated that in the developing embryo, any abnormal modulation of this enzymatic activity may lead to the production of teratogenic effects. In the present study, we investigated the effect of teratogenic doses (inducing about 50% of malformed embryos) of retinoic acid, auranofin and mercuric chloride on PA activity in post-implantation cultured mouse embryos. At the end of the culture period, PA activities of malformed and normal embryos in the same treatment group were compared. PA activities in compound-exposed embryos were also compared with those in untreated controls. The design of the present experiment allowed the identification of the effect of drugs on PA activity and its possible relation with induced malformations. An increased PA activity was observed in malformed embryos treated with mercuric chloride. PA activity was slightly increased in both groups (normal and malformed) exposed to retinoic acid. No effect of auranofin was observed on embryo PA activity. In conclusion, the data do not confirm but they also do not contradict the hypothesis that abnormal levels of PA activity lead to dysmorphogenesis.
Lison
D
D
Industrial Toxicology and Occupational Medicine Unit, UCL, Clos Chapelle-aux-champs, 3054, B-1200 Brussels, Belgium.
Van Maele-Fabry
G
G
Gofflot
F
F
Picard
J J
JJ
Lauwerys
R
R