Water-extracted polysaccharides from Azadirachta indica leaves: Structural features, chemical modification and anti-bovine herpesvirus type 1 (BoHV-1) activity.
In this study, we have analyzed the pectic arabinogalactan isolated from Azadirachta indica and its chemically sulfated derivative. The native polysaccharide had an apparent molecular mass of 80 kDa and is made up of (1→5)-/(1→3,5)-linked α-L-arabinosyl, (1→3)-/(1→6)-/(1→3,6)-linked β-D-galactosyl, and terminal-rhamnosyl and α-l-arabinosyl residues. These macromolecules showed activity against bovine herpesvirus type-1. Their inhibitory concentration 50% values ranging from 31.12 to 105.25 μg/ml were lower than the cytotoxicity values (>1600-1440 μg/ml). The anti-viral effect was exerted during virus adsorption to the cell. Anionic groups in particular the sulfate groups appeared to be very important for the anti-herpetic activity of these polymers.
Saha
Sudipta
S
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapag, Burdwan, WB 713 104, India.
Galhardi
Ligia C F
LC
Yamamoto
Kristie A
KA
Linhares
Rosa Elisa C
RE
Bandyopadhyay
Shruti S
SS
Sinha
Sharmistha
S
Nozawa
Carlos
C
Ray
Bimalendu
B
D000818:Animals; D000838:Anions; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D001681:Biological Assay; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006027:Glycosides / Q000737:chemistry; D007242:Herpesvirus 1, Bovine / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects; D014867:Water / Q000737:chemistry*
D000838:Anions; D000998:Antiviral Agents; D006027:Glycosides; D011134:Polysaccharides; D014867:Water