title: Drosophila Sld5 is essential for normal cell cycle progression and maintenance of genomic integrity.
abstract: Essential for the normal functioning of a cell is the maintenance of genomic integrity. Failure in this process is often catastrophic for the organism, leading to cell death or mis-proliferation. Central to genomic integrity is the faithful replication of DNA during S phase. The GINS complex has recently come to light as a critical player in DNA replication through stabilization of MCM2-7 and Cdc45 as a member of the CMG complex which is likely responsible for the processivity of helicase activity during S phase. The GINS complex is made up of 4 members in a 1:1:1:1 ratio: Psf1, Psf2, Psf3, And Sld5. Here we present the first analysis of the function of the Sld5 subunit in a multicellular organism. We show that Drosophila Sld5 interacts with Psf1, Psf2, and Mcm10 and that mutations in Sld5 lead to M and S phase delays with chromosomes exhibiting hallmarks of genomic instability.
authors: Department of Biology, East Carolina University East Carolina University, Greenville, NC 27858, USA.
authors: Catherine A
authors: CA
authors: Gouge
authors: Tim W
authors: TW
authors: Christensen
mesh_terms: D000483:Alleles; D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D042822:Genomic Instability / Q000235:genetics*; D064110:Minichromosome Maintenance Proteins; D008938:Mitosis; D009154:Mutation; D020798:Two-Hybrid System Techniques
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C489612:MCM10 protein, Drosophila; C552695:Sld5 protein, Drosophila; D064110:Minichromosome Maintenance Proteins