The matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)-based protein peaks of 4448 and 5302 Da are not associated with the presence of Panton-Valentine leukocidin.
The Panton-Valentine leukocidin (PVL) of Staphylococcus aureus plays an important role in the pathogenesis of necrotizing pneumonia and recurrent skin and soft tissue infections. The gene encoding for PVL, lukS/F-PV, is distributed by prophages and can thus spread between isolates. Molecular methods have normally been used to identify lukS/F-PV-positive strains. Recently, however, a matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS)-based method has been described to identify PVL-positive S. aureus strains. The aim of this study was thus to investigate the association of distinct MALDI-TOF MS peaks to the toxin profile in molecularly characterized methicillin-susceptible (MSSA) and methicillin-resistant S. aureus (MRSA) strains harbouring lukS/F-PV. In contrast to the previous results, the MALDI-TOF MS peaks were detected in all 104 recently described molecularly divergent MRSA irrespective of the presence of PVL. Our result indicates that these described peaks seem to be independent of the presence of PVL.
Szabados
Florian
F
Institute for Hygiene and Microbiology, Department of Medical Microbiology, Ruhr-University, Bochum, Germany. Florian.Szabados@rub.de
Becker
Karsten
K
von Eiff
Christof
C
Kaase
Martin
M
Gatermann
Sören
S
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D001427:Bacterial Toxins / Q000032:analysis* / Q000737:chemistry; D001431:Bacteriological Techniques / Q000379:methods*; D005098:Exotoxins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007956:Leukocidins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000737:chemistry*; D037521:Virulence Factors / Q000032:analysis / Q000737:chemistry
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D037521:Virulence Factors