Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model.
We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5 × 10(7) CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0 × 10(7) CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity.
Yamamoto
Sakura
S
Division of Infectious Disease Control, Kobe University Graduate School of Medicine, Kobe, Japan.
Wada
Jun
J
Katayama
Takane
T
Jikimoto
Takumi
T
Nakamura
Masakuni
M
Kinoshita
Shohiro
S
Lee
Kyung-Mi
KM
Kawabata
Masato
M
Shirakawa
Toshiro
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001644:Bifidobacterium / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005408:Flagellin / Q000276:immunology; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology; D014435:Typhoid Fever / Q000276:immunology / Q000517:prevention & control*; D014436:Typhoid-Paratyphoid Vaccines / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011993:Recombinant Fusion Proteins; D014436:Typhoid-Paratyphoid Vaccines; D005408:Flagellin