title: Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation.
abstract: Che-1 is a RNA polymerase II binding protein involved in the regulation of gene transcription and, in response to DNA damage, promotes p53 transcription. In this study, we investigated whether Che-1 regulates mutant p53 expression. We found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo. Notably, loss of Che-1 activates DNA damage checkpoint response and induces transactivation of p73. Therefore, these findings underline the important role that Che-1 has in survival of cells expressing mutant p53.
authors: Department of Therapeutic Programs Development, Regina Elena Cancer Institute, Rome, Italy.
authors: Tiziana
authors: T
authors: Bruno
authors: Agata
authors: A
authors: Desantis
authors: Gianluca
authors: G
authors: Bossi
authors: Silvia
authors: S
authors: Di Agostino
authors: Cristina
authors: C
authors: Sorino
authors: Francesca
authors: F
authors: De Nicola
authors: Simona
authors: S
authors: Iezzi
authors: Annapaola
authors: A
authors: Franchitto
authors: Barbara
authors: B
authors: Benassi
authors: Sergio
authors: S
authors: Galanti
authors: Francesca
authors: F
authors: La Rosa
authors: Aristide
authors: A
authors: Floridi
authors: Alfonso
authors: A
authors: Bellacosa
authors: Claudio
authors: C
authors: Passananti
authors: Giovanni
authors: G
authors: Blandino
authors: Maurizio
authors: M
authors: Fanciulli
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology*; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics; D034741:RNA, Small Interfering; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*; D014183:Transplantation, Heterologous; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: C402637:AATF protein, human; D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C000606460:TP73 protein, human; C000606368:Trp73 protein, mouse; D000072160:Tumor Protein p73; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins