title: A ferricyanide-mediated activated sludge bioassay for fast determination of the biochemical oxygen demand of wastewaters.
abstract: Activated sludge was successfully incorporated as the biocatalyst in the fast, ferricyanide-mediated biochemical oxygen demand (FM-BOD) bioassay. Sludge preparation procedures were optimized for three potential biocatalysts; aeration basin mixed liquor, aerobic digester sludge and return activated sludge. Following a 24h starving period, the return activated sludge and mixed liquor sludges reported the highest oxidative degradation of a standard glucose/glutamic acid (GGA) mixture and the return activated sludge also recorded the lowest endogenous FM-respiration rate. Dynamic working ranges up to 170 mg BOD(5)L(-1) for OECD standard solutions and 300mg BOD(5)L(-1) for GGA were obtained. This is a considerable improvement upon the BOD(5) standard assay and most other rapid BOD techniques. Time-series ferricyanide-mediated oxidation of the OECD(170) standard approached that of the GGA(198) standard after 3-6h. This is noteworthy given the OECD standard is formulated as a synthetic sewage analogue. A highly significant correlation with the BOD(5) standard method (n=35, p<0.001, R=0.952) was observed for a wide diversity of real wastewater samples. The mean degradation efficiency was indistinguishable from that observed for the BOD(5) assay. These results demonstrate that the activated sludge FM-BOD assay may be used for simple, same-day BOD analysis of wastewaters.
authors: Environmental Futures Centre, Griffith University, Gold Coast campus, Qld 4222, Australia.
authors: Mark A
authors: MA
authors: Jordan
authors: David T
authors: DT
authors: Welsh
authors: Peter R
authors: PR
authors: Teasdale
authors: Kylie
authors: K
authors: Catterall
authors: Richard
authors: R
authors: John
mesh_terms: D001681:Biological Assay / Q000379:methods*; D005292:Ferricyanides / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry*; D014865:Waste Disposal, Fluid
chemical_list: D005292:Ferricyanides; D012722:Sewage; D010100:Oxygen