title: Chemical composition and antioxidant and antimycobacterial activities of Bromelia balansae (Bromeliaceae).
abstract: Bromelia balansae (Family Bromeliaceae) is a medicinal plant commonly used in the central region of Brazil as a cough syrup and also eaten roasted. The methanolic extract of ripe fruits was analyzed by chromatographic methods and spectrometrically. Four glycoside flavonols were isolated: kaempferol-3-O-α-L-rhamnopyranoside (1), kaempferol-3-O-α-L-rhamnopyranosyl-(1-->6)-ß-D-glucopyranoside (2), quercetin-3-O-α-L-rhamnopyranosyl-(1-->6)-ß-d-glucopyranoside (3), and kaempferol 3,7-di-O-α-L-rhamnopyranoside (4). The resazurin microtiter assay was used to measure the biological activity in vitro against Mycobacterium tuberculosis. The results showed a moderate activity of the methanolic extract with a minimal inhibitory concentration of 128 μg/mL. Antioxidant activity was evaluated as free radical scavenging capacity and inhibition of peroxidation. Free radical scavenging capacity was assessed by measuring the scavenging activity of methanolic extract and methanolic fraction on 2,2-diphenyl-1-picrylhydrazyl radical. The methanolic extract showed low values of antioxidant activities, whereas the methanolic fraction exhibited free radical scavenging activity ranging from 20.2% to 91.1%, and the inhibition of peroxidation values ranging from 5.6% to 27.5%. This is the first chemical study reported in the literature about this species.
authors: Department of Chemistry, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil. robertacoelho@yahoo.com.br
authors: Roberta Gomes
authors: RG
authors: Coelho
authors: Neli Kika
authors: NK
authors: Honda
authors: Maria do Carmo
authors: Mdo C
authors: Vieira
authors: Rosenei Louzada
authors: RL
authors: Brum
authors: Fernando Rogério
authors: FR
authors: Pavan
authors: Clarice Queico Fujimura
authors: CQ
authors: Leite
authors: Claudia Andréa Lima
authors: CA
authors: Cardoso
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D031231:Bromelia / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D000432:Methanol