Spatio-temporal progression of grey and white matter damage following contusion injury in rat spinal cord.
Cellular mechanisms of secondary damage progression following spinal cord injury remain unclear. We have studied the extent of tissue damage from 15 min to 10 weeks after injury using morphological and biochemical estimates of lesion volume and surviving grey and white matter. This has been achieved by semi-quantitative immunocytochemical methods for a range of cellular markers, quantitative counts of white matter axonal profiles in semi-thin sections and semi-quantitative Western blot analysis, together with behavioural tests (BBB scores, ledged beam, random rung horizontal ladder and DigiGait analysis). We have developed a new computer-controlled electronic impactor based on a linear motor that allows specification of the precise nature, extent and timing of the impact. Initial (15 min) lesion volumes showed very low variance (1.92+/-0.23 mm3, mean+/-SD, n=5). Although substantial tissue clearance continued for weeks after injury, loss of grey matter was rapid and complete by 24 hours, whereas loss of white matter extended up to one week. No change was found between one and 10 weeks after injury for almost all morphological and biochemical estimates of lesion size or behavioural methods. These results suggest that previously reported apparent ongoing injury progression is likely to be due, to a large extent, to clearance of tissue damaged by the primary impact rather than continuing cell death. The low variance of the impactor and the comprehensive assessment methods described in this paper provide an improved basis on which the effects of potential treatment regimes for spinal cord injury can be assessed.
Ek
C Joakim
CJ
Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
Habgood
Mark D
MD
Callaway
Jennifer K
JK
Dennis
Ross
R
Dziegielewska
Katarzyna M
KM
Johansson
Pia A
PA
Potter
Ann
A
Wheaton
Benjamin
B
Saunders
Norman R
NR
D000818:Animals; D001369:Axons / Q000473:pathology; D001522:Behavior, Animal; D015153:Blotting, Western; D002452:Cell Count; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology*; D003288:Contusions / Q000378:metabolism / Q000473:pathology*; D018450:Disease Progression; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D009474:Neurons / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors