RNAi-mediated silencing of a novel Ascaris suum gene expression in infective larvae.
In the present study, the potential of RNA interference (RNAi) as a gene silencing tool and the resultant effects on Ascaris suum larval development was examined by targeting a gene (represented by the EST 06G09) specifically expressed in the infective larvae of A. suum. BALB/c mice were infected with RNAi-treated larvae. The results showed that the target gene was silenced after soaking for 72 h, and the survival rate of the RNAi-treated larvae was reduced by 17.25% (P<0.01). A significant difference (P<0.05) was detected in the numbers of larvae collected from the livers and lungs of infected mice 4 days after infection with untreated larvae (164.29 ± 21.51) and RNAi-treated larvae (71.43 ± 14.35). Significant differences (P<0.01) were also found in the body length and width between untreated larvae (480 ± 105.77 μm for length and 23.93 ± 3.72 μm for width) and RNAi-treated larvae (400.57 ± 71.31 μm for length and 20.20 ± 2.43 μm for width). These results show that the gene represented by EST 06G09 may play a role in the development of A. suum larvae.
Xu
M J
MJ
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, 510642, People's Republic of China.
Chen
N
N
Song
H Q
HQ
Lin
R Q
RQ
Huang
C Q
CQ
Yuan
Z G
ZG
Zhu
X Q
XQ
D000818:Animals; D000871:Anthelmintics / Q000378:metabolism*; D001196:Ascariasis / Q000469:parasitology; D017165:Ascaris suum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D001688:Biological Products / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D020868:Gene Silencing; D007814:Larva / Q000235:genetics; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D051379:Mice; D034741:RNA, Small Interfering / Q000378:metabolism*
D000871:Anthelmintics; D001688:Biological Products; D034741:RNA, Small Interfering