title: A multi-target screening analysis in human plasma using fast liquid chromatography-hybrid tandem mass spectrometry (Part II).
abstract: OBJECTIVE
  Perform a comparison of results obtained with a LC-MS/MS method and a Remedi® instrument on clinical serum samples.
  
  
  METHODS
  Results obtained on 146 selected plasma samples were compared between the two methods.
  
  
  RESULTS
  On the 336 positive identifications, 89% were obtained using the LC-MS/MS technique and 57% by the LC-DAD. Benzodiazepines were well recognized by LC-MS/MS. For some compounds such as antidepressant agents, sensitivity was improved using LC-MS/MS. Moreover, this method extended the panel of drugs detected in clinical toxicology.
  
  
  CONCLUSIONS
  The new software platform developed for screening and identification of small molecules (SmileMS) allows an easy and reproducible detection of drugs and toxic compounds in blood for general unknown screening. It offers automated generation of reports, which makes the LC-MS/MS easier to use without having specialised skills in mass spectrometry. This LC-MS/MS screening method will be a reliable alternative to the Remedi® instrument in the global process of screening in emergency clinical toxicology laboratories.
authors: Department of Genetics and Laboratory Medicine, Geneva University Hospital, Switzerland.
authors: V
authors: V
authors: Viette
authors: D
authors: D
authors: Guillarme
authors: R
authors: R
authors: Mylonas
authors: Y
authors: Y
authors: Mauron
authors: M
authors: M
authors: Fathi
authors: S
authors: S
authors: Rudaz
authors: D
authors: D
authors: Hochstrasser
authors: J-L
authors: JL
authors: Veuthey
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000097:blood; D053719:Tandem Mass Spectrometry
chemical_list: D004364:Pharmaceutical Preparations