title: A mouse model of autoimmune pancreatitis with salivary gland involvement triggered by innate immunity via persistent exposure to avirulent bacteria.
abstract: The pathogenesis of autoimmune pancreatitis (AIP) remains unknown. Here, we investigated the possible involvement of chronic, persistent exposure to avirulent bacteria in the pathogenesis of AIP. C57BL/6 mice were inoculated with heat-killed Escherichia coli weekly for 8 weeks. At 1 week and up to 12 months after the final inoculation, the mice were killed to obtain samples. At 1 week after the final E. coli inoculation, marked cellular infiltration with fibrosis was observed in the exocrine pancreas. Cellular infiltration in the exocrine pancreas was still observed up to 12 months after the completion of E. coli inoculation. At 10 months after the final inoculation, duct-centric fibrosis became obvious. Inflammation around the ducts in the salivary glands was also observed. Furthermore, sera from heat-killed E. coli-inoculated mice possessed anti-carbonic anhydrase, anti-lactoferrin, and antinuclear antibodies. Exposure to E. coli-triggered AIP-like pancreatitis in C57BL/6 mice. We propose a hypothetical mechanism for AIP pathogenesis. During the initiation phase, silently infiltrating pathogen-associated molecular patterns (PAMP) and/or antigen(s) such as avirulent bacteria might trigger and upregulate the innate immune system. Subsequently, the persistence of such PAMP attacks or stimulation by molecular mimicry upregulates the host immune response to the target antigen. These slowly progressive steps may lead to the establishment of AIP and associated extrapancreatic lesions. Our model might be useful for clarifying the pathogenesis of AIP.
authors: Department of Microbiology and Immunology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan. haruta@research.twmu.ac.jp
authors: Ikuko
authors: I
authors: Haruta
authors: Naoko
authors: N
authors: Yanagisawa
authors: Shunji
authors: S
authors: Kawamura
authors: Toru
authors: T
authors: Furukawa
authors: Kyoko
authors: K
authors: Shimizu
authors: Hidehito
authors: H
authors: Kato
authors: Makio
authors: M
authors: Kobayashi
authors: Keiko
authors: K
authors: Shiratori
authors: Junji
authors: J
authors: Yagi
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D046790:Pancreas, Exocrine / Q000276:immunology / Q000473:pathology; D010195:Pancreatitis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D012466:Salivary Gland Diseases / Q000276:immunology* / Q000382:microbiology; D012469:Salivary Glands / Q000276:immunology / Q000473:pathology*
chemical_list: D000974:Antibodies, Antinuclear