title: [Effects of retinoic acid receptor agonist and retinoid X receptor agonist on the expression of IL-4 and IFN-gamma in cord blood mononuclear cells].
abstract: OBJECTIVE
  To observe the effects of retinoic acid receptors agonist TTNPB and retinoid X receptors agonist MA on both protein and gene expression of IFN-gamma and IL-4 in cultured cord blood mononuclear cells.
  
  
  METHODS
  Observing both the protein content and gene expression of IFN-gamma and IL-4 with ELISA and fluorescent real-time quantitative PCR in vitro.
  
  
  RESULTS
  It was indicated by ELISA that 10(-8), 10(-6), 10(-5) and 10(-4) mol/L TTNPB and 10(-8), 10(-5) and 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4 significantly in comparison with the control (P < 0.05), and indicated by RT-PCR that 10(-4) mol/L MA decreased the ratio of IFN-gamma/IL-4mRNA significantly and 10(-5) mol/L TTNPB and 10(-7), 10(-6) mol/L MA increased the ratio of IFN-gamma/IL-4mRNA significantly in comparison with the control (P < 0.05).
  
  
  CONCLUSIONS
  The ratio of IFN-gamma/IL-4 could be down-regulated by proper concentration of TTNPB and MA at the protein level, while the ratio of IFN-gamma/IL-4mRNA was differently influenced by various concentration of TTNPB and MA at the gene level.
authors: Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China. zdy213@126.com
authors: Dongyang
authors: D
authors: Zhu
authors: Lili
authors: L
authors: Wang
authors: Yun
authors: Y
authors: Li
authors: Yanfeng
authors: Y
authors: Zhu
authors: Pinnan
authors: P
authors: Zhao
mesh_terms: D001565:Benzoates / Q000494:pharmacology; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000819:agonists* / Q000502:physiology; D047488:Retinoid X Receptors / Q000819:agonists* / Q000502:physiology; D012176:Retinoids / Q000494:pharmacology
chemical_list: D001565:Benzoates; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D012176:Retinoids; D015847:Interleukin-4; C038864:4-(2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic acid; D007371:Interferon-gamma