HIV type 1 pol gene diversity and genotypic antiretroviral drug resistance mutations in Malabo, Equatorial Guinea.
In this study, HIV strains circulating among military personnel were characterized, in Malabo, the capital city of Equatorial Guinea. One sample was found to be HIV-2 group A while a high degree of genetic diversity was recorded in the pol region of 41 HIV-1-positive samples. CRF02_AG accounted for 53.7% of the strains, and 11 different variants were obtained in the remaining 19 samples: subtype G (n = 3), A3 (n = 2), C (n = 2), CRF26_A5U (n = 2), F2 (n = 1), CRF06 (n = 1), CRF09 (n = 1), CRF11 (n = 1), CRF22 (n = 1), and divergent subtype A (n = 1) and F (n = 1). One strain could not be classified and three were unique recombinants. Analysis of antiretroviral drug resistance mutations revealed two patients each harboring one major mutation, M46I in protease and D67N in reverse transcriptase sequences, respectively. The high genetic diversity and emerging ARV resistance mutations call for frequent surveys and appropriate monitoring of ARV considering the increasing access to ARV in the country.
Djoko
Cyrille F
CF
Biotechnology Center & Department of Biochemistry, University of Yaounde I, Yaounde, Cameroon.
Wolfe
Nathan D
ND
Vidal
Nicole
N
Tamoufe
Ubald
U
Montavon
Celine
C
LeBreton
Matthew
M
Pike
Brian L
BL
Fair
Joseph
J
Mbacham
Wilfred F
WF
Benito
Alogos
A
Rimoin
Anne W
AW
Saylors
Karen
K
Mpoudi-Ngole
Eitel
E
Grillo
Michael P
MP
Peeters
Martine
M
D000293:Adolescent; D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use; D024882:Drug Resistance, Viral / Q000235:genetics*; D016703:Equatorial Guinea; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008889:Military Personnel; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D020539:Sequence Analysis, Protein; D055815:Young Adult; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
D019380:Anti-HIV Agents; D054302:pol Gene Products, Human Immunodeficiency Virus