Stereocomplexed poly(lactic acid)-poly(ethylene glycol) nanoparticles with dual-emissive boron dyes for tumor accumulation.
Responsive biomaterials play important roles in imaging, diagnostics, and therapeutics. Polymeric nanoparticles (NPs) containing hydrophobic and hydrophilic segments are one class of biomaterial utilized for these purposes. The incorporation of luminescent molecules into NPs adds optical imaging and sensing capability to these vectors. Here we report on the synthesis of dual-emissive, pegylated NPs with "stealth"-like properties, delivered intravenously (IV), for the study of tumor accumulation. The NPs were created by means of stereocomplexation using a methoxy-terminated polyethylene glycol and poly(D-lactide) (mPEG-PDLA) block copolymer combined with iodide-substituted difluoroboron dibenzoylmethane-poly(L-lactide) (BF2dbm(I)PLLA). Boron nanoparticles (BNPs) were fabricated in two different solvent compositions to study the effects on BNP size distribution. The physical and photoluminescent properties of the BNPs were studied in vitro over time to determine stability. Finally, preliminary in vivo results show that stereocomplexed BNPs injected IV are taken up by tumors, an important prerequisite to their use as hypoxia imaging agents in preclinical studies.
Kersey
Farrell R
FR
Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, Virginia 22904, USA.
Zhang
Guoqing
G
Palmer
Gregory M
GM
Dewhirst
Mark W
MW
Fraser
Cassandra L
CL
D000042:Absorption; D000818:Animals; D001692:Biological Transport; D001895:Boron / Q000032:analysis* / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D057054:Molecular Imaging; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry*; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics*; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis; D013237:Stereoisomerism
D004396:Coloring Agents; D011091:Polyesters; D011108:Polymers; D012997:Solvents; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D001895:Boron