Antileishmanial activity of a guaianolide from Tanacetum parthenium (L.) Schultz Bip.
Leishmaniasis is one of the major infectious diseases affecting the poorest regions of the world. The present study evaluated the antileishmanial activity of a guaianolide purified from the hydroalcoholic extract of aerial parts of Tanacetum parthenium (L.) Schultz Bip. The isolated compound showed activity against the promastigote form of Leishmania amazonensis, with 50% inhibition (IC(50)) of cell growth at a concentration of 2.6 μg/ml. For the intracellular amastigote form, this guaianolide reduced by 10% the survival index of parasites in macrophages when it was used at 20.0 μg/ml. The selective index (SI) ratio (CC(50) for J774G8 cells/IC(50) for protozoans) was 385, showing that it is more selective against the parasite than mammalian cells. Morphological alterations of protozoans treated with IC(50) included changes in size, shape, and structure (more than one nucleus and flagellum) under both light and scanning electron microscopies.
da Silva
Bruna Polacchine
BP
Programa de Pós-graduação em Ciências Farmacêuticas da Universidade Estadual de Maringá, PR, Brazil.
Cortez
Diogenes Aparício
DA
Violin
Thaisa Yume
TY
Dias Filho
Benedito Prado
BP
Nakamura
Celso Vataru
CV
Ueda-Nakamura
Tânia
T
Ferreira
Izabel Cristina Piloto
IC
D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D017737:Macrophages, Peritoneal / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D045789:Sesquiterpenes, Guaiane / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D020903:Tanacetum parthenium / Q000737:chemistry*
D000981:Antiprotozoal Agents; D010936:Plant Extracts; D045789:Sesquiterpenes, Guaiane