title: Construction of an infectious cDNA clone of avian hepatitis E virus (avian HEV) recovered from a clinically healthy chicken in the United States and characterization of its pathogenicity in specific-pathogen-free chickens.
abstract: A genetically distinct strain of avian hepatitis E virus (avian HEV-VA strain) was isolated from a healthy chicken in Virginia, and thus it is important to characterize and compare its pathogenicity with the prototype strain (avian HEV-prototype) isolated from a diseased chicken. Here we first constructed an infectious clone of the avian HEV-VA strain. Capped RNA transcripts from the avian HEV-VA clone were replication-competent after transfection of LMH chicken liver cells. Chickens inoculated intrahepatically with RNA transcripts of avian HEV-VA clone developed active infection as evidenced by fecal virus shedding, viremia, and seroconversion. To characterize the pathogenicity, RNA transcripts of both avian HEV-VA and avian HEV-prototype clones were intrahepatically inoculated into the livers of chickens. Avian HEV RNA was detected in feces, serum and bile samples from 10/10 avian HEV-VA-inoculated and 9/9 avian HEV-prototype-inoculated chickens although seroconversion occurred only in some chickens during the experimental period. The histopathological lesion scores were lower for avian HEV-VA group than avian HEV-prototype group in the liver at 3 and 5 weeks post-inoculation (wpi) and in the spleen at 3 wpi, although the differences were not statistically significant. The liver/body weight ratio, indicative of liver enlargement, of both avian HEV-VA and avian HEV-prototype groups were significantly higher than that of the control group at 5 wpi. Overall, the avian HEV-VA strain still induces histological liver lesions even though it was isolated from a healthy chicken. The results also showed that intrahepatic inoculation of chickens with RNA transcripts of avian HEV infectious clone may serve as an alternative for live virus in animal pathogenicity studies.
authors: Center for Molecular Medicine and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0913, USA.
authors: Hyuk Moo
authors: HM
authors: Kwon
authors: Tanya
authors: T
authors: LeRoith
authors: R S
authors: RS
authors: Pudupakam
authors: F William
authors: FW
authors: Pierson
authors: Yao-Wei
authors: YW
authors: Huang
authors: Barbara A
authors: BA
authors: Dryman
authors: Xiang-Jin
authors: XJ
authors: Meng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D016751:Hepatitis E / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D006524:Hepatitis, Viral, Animal / Q000503:physiopathology / Q000821:virology*; D052678:Hepevirus / Q000235:genetics / Q000472:pathogenicity*; D008099:Liver / Q000473:pathology / Q000821:virology; D011201:Poultry Diseases / Q000473:pathology / Q000821:virology*; D012315:RNA Caps / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D014768:Virginia; D017201:Virus Shedding
chemical_list: D018076:DNA, Complementary; D012315:RNA Caps