[A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi].
OBJECTIVE
To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE, CRF07_BC, CRF08_BC, B, and C strains prevailing in Guangxi.


METHODS
Subtype-specific primers were designed for these subtypes based on their gag sequences. The subtypes of HIV-1 samples from Guangxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis, respectively, and then the sensitivity and the specificity of nested multiplex PCR were calculated.


RESULTS
Nested multiplex PCR could correctly classify the 5 known-subtype samples, and were not reactive to all HIV-negative samples. Of the 72 HIV-positive samples, 66 were correctly identified as CRF01_AE, CRF07_BC, CRF08_BC, and B by this assay, giving a sensitivity of 91.7% (66/72), and a specificity of 100%.


CONCLUSIONS
This assay is a simple, fast, and cost-effective subtyping method for HIV-1 CRF01-AE, CRF07_BC, CRF08_BC, and B strains prevailing in Guangxi.
Su
Qi-jian
QJ
Center for AIDS Research & School of Public Health, Guangxi Medical University, Nanning, China.
Zhou
Ping
P
Bi
Zhi-you
ZY
Xiao
Xin
X
Wu
Shu-zhi
SZ
Cen
Ping
P
Deng
Wei
W
Shao
Yi-ming
YM
Liang
Hao
H
D002681:China; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*
D017931:DNA Primers