Genome editing with engineered zinc finger nucleases.
Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells - this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair. Such 'genome editing' is now established in human cells and a number of model organisms, thus opening the door to a range of new experimental and therapeutic possibilities.
Urnov
Fyodor D
FD
Sangamo BioSciences Inc., Richmond, California 94804, USA.
Rebar
Edward J
EJ
Holmes
Michael C
MC
Zhang
H Steve
HS
Gregory
Philip D
PD
D000818:Animals; D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D016335:Zinc Fingers
D004720:Endonucleases