Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord.
Differences in the regulation of surface molecule expression and functional activity of microglia, the resident immune effector elements of the central nervous system (CNS), might give important insights into understanding the predilection sites of some diseases within the CNS. Therefore, canine microglial cells in relation to different topographical regions within the healthy CNS were evaluated ex vivo from the brain, cervical, and thoracolumbar spinal cord using density gradient centrifugation and flow cytometry in a homogenous dog population. Immunophenotypical characterization showed physiological regional differences for B7-1, CD14, CD44, CD1c, CD18, CD11b, and CD11c. Both, phagocytosis and ROS generation revealed differences between the brain, cervical, and thoracolumbar spinal cord. Our results emphasize that microglia displays physiological topographical regional differences within the CNS. The dog seems to be an ideal model to further investigate the role of microglia in focal pathological conditions of the spinal cord.
Ensinger
Eva-Maria
EM
Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Bünteweg 9, D-30559 Hannover, Germany.
Boekhoff
Theda M A
TM
Carlson
Regina
R
Beineke
Andreas
A
Rohn
Karl
K
Tipold
Andrea
A
Stein
Veronika M
VM
D000818:Animals; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D016130:Immunophenotyping / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism
D017382:Reactive Oxygen Species