L-cysteine-capped CdTe quantum dots as a fluorescence probe for determination of cardiolipin.
This paper described the investigation of surface-modified quantum dots (QDs) as a fluorescence probe for the detection of cardiolipin. A single-step method for preparation of non-toxic and photo-stable cadmium telluride (CdTe) QDs capped by L-cysteine in aqueous solution was developed. The prepared QDs were characterized by high-resolution transmission electron microscopy, X-ray diffraction spectrometry, Fourier transform infrared spectrometry and spectrofluorometry. These functional QDs were used as a fluorescence probe for cardiolipin determination based on the fluorescence quenching. The optimum fluorescence intensity was found to be at pH 7.4 with QDs concentration of 4 x 10(-5) mol L(-1). The effect of other phospholipids on the intensity of CdTe QDs showed a low interference response. Under optimized conditions, the quenched fluorescence intensity was linear with the concentration of cardiolipin in the range of 1.33 x 10(-7) - 10.4 x 10(-7) mol L(-1) (r = 0.9976) and a detection limit (S/N = 3) of 18.5 nmol L(-1). The proposed method was applied to the determination of cardiolipin content of HepG2 cell samples before and after oxidative stress with satisfactory results.
Zhao
Wenfeng
W
Department of Chemistry, The University of Hong Kong, Hong Kong SAR, PR China. zhaowf@hkusua.hku.hk
Fung
Yingsing
Y
O
Waisum
W
Cheung
M P L
MP
D019187:Cadmium Compounds / Q000737:chemistry*; D002308:Cardiolipins / Q000032:analysis*; D003545:Cysteine / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D057230:Limit of Detection; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013691:Tellurium / Q000737:chemistry*; D014961:X-Ray Diffraction
D019187:Cadmium Compounds; D002308:Cardiolipins; D003545:Cysteine; D013691:Tellurium; C028337:cadmium telluride