A novel, thermostable manganese-containing superoxide dismutase from Bacillus licheniformis.
A new, thermostable superoxide dismutase (SOD) from Bacillus licheniformis M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE, the enzyme was composed of two subunits of 21.5 kDa each. The SOD was inhibited only by NaN(3), which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at pH 8 and 55°C. The half-life of the SOD was 10 min at 95°C.
Boyadzhieva
Ivanka Petrova
IP
Department of Extremophilic Bacteria, Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev Str., 1113, Sofia, Bulgaria. petrovaim@abv.bg
Atanasova
Miroslava
M
Emanuilova
Elka
E
D001407:Bacillus / Q000201:enzymology* / Q000302:isolation & purification; D002031:Bulgaria; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000378:metabolism; D004795:Enzyme Stability; D006207:Half-Life; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification; D019810:Sodium Azide / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
D004791:Enzyme Inhibitors; D021122:Protein Subunits; D019810:Sodium Azide; D013482:Superoxide Dismutase