title: miR-204 is required for lens and retinal development via Meis2 targeting.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that have important roles in the regulation of gene expression. The roles of individual miRNAs in controlling vertebrate eye development remain, however, largely unexplored. Here, we show that a single miRNA, miR-204, regulates multiple aspects of eye development in the medaka fish (Oryzias latipes). Morpholino-mediated ablation of miR-204 expression resulted in an eye phenotype characterized by microphthalmia, abnormal lens formation, and altered dorsoventral (D-V) patterning of the retina, which is associated with optic fissure coloboma. Using a variety of in vivo and in vitro approaches, we identified the transcription factor Meis2 as one of the main targets of miR-204 function. We show that, together with altered regulation of the Pax6 pathway, the abnormally elevated levels of Meis2 resulting from miR-204 inactivation are largely responsible for the observed phenotype. These data provide an example of how a specific miRNA can regulate multiple events in eye formation; at the same time, they uncover an as yet unreported function of Meis2 in the specification of D-V patterning of the retina.
authors: Telethon Institute of Genetics and Medicine, 80131 Naples, Italy.
authors: Ivan
authors: I
authors: Conte
authors: Sabrina
authors: S
authors: Carrella
authors: Raffaella
authors: R
authors: Avellino
authors: Marianthi
authors: M
authors: Karali
authors: Raquel
authors: R
authors: Marco-Ferreres
authors: Paola
authors: P
authors: Bovolenta
authors: Sandro
authors: S
authors: Banfi
mesh_terms: D000818:Animals; D002460:Cell Line; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D038081:Organogenesis / Q000235:genetics; D009990:Oryzias / Q000196:embryology / Q000235:genetics; D012160:Retina / Q000196:embryology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029941:Fish Proteins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D014157:Transcription Factors