title: Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
abstract: A large and diverse array of chemoattractants control leukocyte trafficking, but how these apparently redundant signals collaborate in vivo is still largely unknown. We previously demonstrated an absolute requirement for the lipid chemoattractant leukotriene B(4) (LTB(4)) and its receptor BLT1 for neutrophil recruitment into the joint in autoantibody-induced arthritis. We now demonstrate that BLT1 is required for neutrophils to deliver IL-1 into the joint to initiate arthritis. IL-1-expressing neutrophils amplify arthritis through the production of neutrophil-active chemokines from synovial tissue cells. CCR1 and CXCR2, two neutrophil chemokine receptors, operate nonredundantly to sequentially control the later phase of neutrophil recruitment into the joint and mediate all neutrophil chemokine activity in the model. Thus, we have uncovered a complex sequential relationship involving unique contributions from the lipid mediator LTB(4), the cytokine IL-1, and CCR1 and CXCR2 chemokine ligands that are all absolutely required for effective neutrophil recruitment into the joint.
authors: Division of Rheumatology, Allergy, and Immunology, Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
authors: Richard C
authors: RC
authors: Chou
authors: Nancy D
authors: ND
authors: Kim
authors: Christian D
authors: CD
authors: Sadik
authors: Edward
authors: E
authors: Seung
authors: Yinan
authors: Y
authors: Lan
authors: Michael H
authors: MH
authors: Byrne
authors: Bodduluri
authors: B
authors: Haribabu
authors: Yoichiro
authors: Y
authors: Iwakura
authors: Andrew D
authors: AD
authors: Luster
mesh_terms: D000818:Animals; D001168:Arthritis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis / Q000276:immunology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D053582:Interleukin-1alpha / Q000172:deficiency / Q000276:immunology*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000172:deficiency / Q000276:immunology*; D007975:Leukotriene B4 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology*; D054389:Receptors, CCR1 / Q000276:immunology; D023063:Receptors, Interleukin-8B / Q000276:immunology; D018077:Receptors, Leukotriene / Q000172:deficiency / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology
chemical_list: C515301:Ccr1 protein, mouse; D018925:Chemokines; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D054389:Receptors, CCR1; D023063:Receptors, Interleukin-8B; D018077:Receptors, Leukotriene; C083173:leukotriene C4 receptor; D007975:Leukotriene B4