[Correlation between ethambutol-resistance and embB gene mutation in Mycobacterium tuberculosis].
OBJECTIVE
To analyze the characteristics of embB gene mutation in ethambutol-resistant Mycobacterium tuberculosis (M. TB) strains isolated from patients in Xi'an for establishing a rapid method to detect the mutation of embB gene in M. TB.


METHODS
The embB gene of 104 Mycobacterium tuberculosis isolates was detected by PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP).


RESULTS
Taking strain H37Rv as a control, the SSCP and RFLP profiles of embB of 35 drug-sensitive strains in 104 Mycobacterium tuberculosis isolates were normal. The display of SSCP profiles in 39 of 69 (56.52%) ethambutol-resistant isolates and the display of RFLP profiles in 19 of 69 (27.54%) ethambutol-resistant isolates were abnormal.


CONCLUSIONS
The EMB-resistance of M. TB strains isolated from patients in Xi'an is related to the mutation of embB gene, PCR-SSCP and PCR-RFLP are sensitive methods for detecting ethambutol-resistance of M. tuberculosis.
Li
Wei
W
Department of Immunology and Pathogen Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. liviy@163.com
Xue
Xin
X
Chu
Yonglie
Y
Qiu
Yi
Y
Song
Juan
J
Zheng
Jianwu
J
D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004977:Ethambutol / Q000494:pharmacology*; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics
D004977:Ethambutol; C540269:EmbB protein, Mycobacterium tuberculosis; D010430:Pentosyltransferases