title: Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution.
abstract: BACKGROUND
  Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage.
  
  
  METHODS
  Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a 'back table' intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES (n=6 per group): Group 1, 0%; Group 2, 2.5%; Group 3, 5%; Group 4, 10%. Energetics, oxidative stress, and morphology were assessed over a 24h time-course of cold storage.
  
  
  RESULTS
  Overall, the 5% HES solution, Group 3, demonstrated superior energetic status (ATP and total adenylates) compared to all groups, P<0.05. Malondialdehyde levels indicated a reduction in oxidative stress in Groups 3 and 4 (P<0.05). After 12h, median modified Parks' grades for Groups 2 and 3 were significantly lower than Groups 1 and 4, P<0.05.
  
  
  CONCLUSIONS
  Our data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal.
authors: Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
authors: Matthew S
authors: MS
authors: Kokotilo
authors: Jodi
authors: J
authors: Carter
authors: Aducio
authors: A
authors: Thiesen
authors: Ming H
authors: MH
authors: Chen
authors: Angela
authors: A
authors: Ochs
authors: Rachel G
authors: RG
authors: Khadaroo
authors: Thomas A
authors: TA
authors: Churchill
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019188:Organ Preservation Solutions / Q000737:chemistry*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D019188:Organ Preservation Solutions; D008315:Malondialdehyde; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate