title: In situ muGISAXS: I. Experimental setup for submicron study of protein nucleation and growth.
abstract: In this study, we used microbeam grazing-incidence small-angle x-ray scattering (muGISAXS) to investigate in situ protein nucleation and crystal growth assisted by a protein nanotemplate, and introduced certain innovations to improve the method. Our aim was to understand the protein nanotemplate method in detail, as this method has been shown to be capable of accelerating and increasing crystal size and quality, as well as inducing crystallization of proteins that are not crystallizable by classical methods. The nanotemplate experimental setup was used for drops containing growing protein crystals at different stages of nucleation and growth. Two model proteins, lysozyme and thaumatin, were used under unique flow conditions to differentially probe protein crystal nucleation and growth.
authors: Nanoworld Institute, CIRSDNNOB-University of Genoa, Genoa, Italy.
authors: Ronald
authors: R
authors: Gebhardt
authors: Eugenia
authors: E
authors: Pechkova
authors: Christian
authors: C
authors: Riekel
authors: Claudio
authors: C
authors: Nicolini
mesh_terms: D000818:Animals; D002645:Chickens; D003460:Crystallization; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010940:Plant Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D010940:Plant Proteins; C003427:thaumatin protein, plant; D009113:Muramidase