title: Seizures during pregnancy modify the development of hippocampal interneurons of the offspring.
abstract: We investigated the effect of epileptic seizures during pregnancy on hippocampal expression of calcium-binding proteins in the offspring. Female Wistar rats were submitted to the pilocarpine model and mated during the chronic period. Seizure frequency was monitored over the entire pregnancy. Pups were perfused at postnatal days 6 and 13, and the brains processed for Nissl staining and immunohistochemistry for NeuN, calbindin, calretinin, and parvalbumin. Number of stained cells in the hippocampus was estimated through stereological methods. Our results showed a decrease in epileptic seizure frequency during pregnancy. No differences were observed in NeuN-positive, CR-positive cells, and Nissl-stained hippocampal neurons between the groups. However, there was a significant decrease in calbindin-positive cells (P=0.005) and a significant increase in parvalbumin-positive cells (P=0.02) in the experimental group when compared with the control group. These results suggest that seizures during pregnancy affect the development of specific hippocampal interneurons of the offspring.
authors: Disciplina de Neurologia Experimental, Universidade Federal de São Paulo, São Paulo, Brasil.
authors: Tiago Gurgel
authors: TG
authors: do Vale
authors: Alexandre Valotta
authors: AV
authors: da Silva
authors: Daiana Correia
authors: DC
authors: Lima
authors: Eliângela
authors: E
authors: de Lima
authors: Laila Brito
authors: LB
authors: Torres
authors: Ana Carolina
authors: AC
authors: Cossa
authors: Elaine Menezes
authors: EM
authors: de Oliveira
authors: Francisco Romero
authors: FR
authors: Cabral
authors: Esper Abrão
authors: EA
authors: Cavalheiro
authors: Maria da Graça
authors: Mda G
authors: Naffah-Mazzacoratti
authors: Débora
authors: D
authors: Amado
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D006624:Hippocampus / Q000254:growth & development* / Q000473:pathology*; D007395:Interneurons / Q000473:pathology*; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010862:Pilocarpine; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000473:pathology*
chemical_list: D002135:Calcium-Binding Proteins; D010862:Pilocarpine; D010751:Phosphopyruvate Hydratase