Immunohistochemical analysis of developmental neural antigen expression in the balloon cells of focal cortical dysplasia.
Balloon cells (BC) are the histological hallmarks of focal cortical dysplasia (FCD). Expression of the neural stem cell surface marker CD133 and other developmental markers was studied in the BC of FCD using formalin-fixed paraffin-embedded tissue from nine patients with FCD. Labeling indexes were calculated for all antibodies. BC were easily identified at the gray-white matter junction and they extended into the white matter. Immunoreactivity in BC was found for the following antigens in nine patients: CD133 (six patients; 22.2 ± 7.7%), CD34 (two patients; 0.4 ± 0.3%), nestin (nine patients; 37.6 ± 8.5%), vimentin (eight patients; 59.2 ± 8.7%), glial fibrillary acid protein (six patients; 34.3 ± 10.4%), microtubule-associated protein 2 (four patients; 8.3 ± 5.0%), neurofilament-middle/high (five patients; 10.2 ± 4.6%) and synaptophysin (three patients; 4.2 ± 3.3%). Neuronal nuclei (NeuN, neuron specific nuclear protein) was not expressed in BC of any patient. The results of this study suggest that BC in patients with FCD originate from glioneuronal precursor cells and that developmental defects of neuronal and glial specifications are important in the histogenesis of FCD.
Han
Chang-Woo
CW
Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea.
Min
Byung-Woo
BW
Kim
Young
Y
Jeong
Eun-Hui
EH
Park
Chang-Soo
CS
Woo
Young-Jong
YJ
Kim
Hyung-Seok
HS
Lee
Min-Cheol
MC
D000071916:AC133 Antigen; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D002648:Child; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D054220:Malformations of Cortical Development / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000378:metabolism; D013702:Temporal Lobe / Q000378:metabolism* / Q000473:pathology
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C000606023:PROM1 protein, human; D010455:Peptides