Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity.
Interleukin-10 is a pivotal determinant of virus clearance or persistence. Two human herpesviruses, Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) are unique among persistent viruses because they not only trigger production of host IL-10, but both viruses also encode homologs of IL-10 that are expressed during infection. Because anti-human IL-10 antibodies have diagnostic value and therapeutic potential for many chronic infections, cross-reactivity with ebvIL-10 and cmvIL-10 was evaluated in this study. Six of seven anti-hIL-10 antibodies tested recognized ebvIL-10 and neutralized its immunosuppressive activity. In contrast, cmvIL-10 was neither recognized nor neutralized by any anti-human IL-10 antibody. These findings demonstrate that IL-10-neutralizing treatments in HCMV- or EBV-infected patients may require consideration of the contribution of viral IL-10 to disease pathology.
Brodeur
Noelle D
ND
Department of Biology, University of San Francisco, San Francisco, CA 94117, United States.
Spencer
Juliet V
JV
D000906:Antibodies / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology* / Q000472:pathogenicity; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D009500:Neutralization Tests; D014764:Viral Proteins / Q000276:immunology*
D000906:Antibodies; D057134:Antibodies, Neutralizing; C508609:IL10 protein, human; D014764:Viral Proteins; D016753:Interleukin-10