The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney.
A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.
Dankers
Patricia Y W
PY
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, NL-9713 GZ Groningen, The Netherlands. p.y.w.dankers@tue.nl
Boomker
Jasper M
JM
Huizinga-van der Vlag
Ali
A
Smedts
Frank M M
FM
Harmsen
Martin C
MC
van Luyn
Marja J A
MJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000378:metabolism; D051398:Aquaporin 1 / Q000378:metabolism; D021522:Bioartificial Organs; D018826:CD13 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D000071858:Epithelial Cell Adhesion Molecule; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D051272:Glucose Transporter Type 1 / Q000378:metabolism; D006801:Humans; D007668:Kidney; D007684:Kidney Tubules / Q000166:cytology*; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics; D008567:Membranes, Artificial; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry; D010477:Perfusion; D010750:Phosphoproteins / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011744:Pyrimidinones / Q000737:chemistry; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D062826:Zonula Occludens-1 Protein; D005723:gamma-Glutamyltransferase / Q000378:metabolism
C109276:2-ureido-4-pyrimidone; C496020:AQP1 protein, human; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D016326:Extracellular Matrix Proteins; D051272:Glucose Transporter Type 1; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008567:Membranes, Artificial; D010750:Phosphoproteins; D011091:Polyesters; D011108:Polymers; D011744:Pyrimidinones; C495677:SLC2A1 protein, human; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D051398:Aquaporin 1; C016240:polycaprolactone; D005723:gamma-Glutamyltransferase; C554782:gamma-glutamyltransferase, human; D000469:Alkaline Phosphatase; D018826:CD13 Antigens; D000254:Sodium-Potassium-Exchanging ATPase