Colour-barcoded magnetic microparticles for multiplexed bioassays.
Encoded particles have a demonstrated value for multiplexed high-throughput bioassays such as drug discovery and clinical diagnostics. In diverse samples, the ability to use a large number of distinct identification codes on assay particles is important to increase throughput. Proper handling schemes are also needed to readout these codes on free-floating probe microparticles. Here we create vivid, free-floating structural coloured particles with multi-axis rotational control using a colour-tunable magnetic material and a new printing method. Our colour-barcoded magnetic microparticles offer a coding capacity easily into the billions with distinct magnetic handling capabilities including active positioning for code readouts and active stirring for improved reaction kinetics in microscale environments. A DNA hybridization assay is done using the colour-barcoded magnetic microparticles to demonstrate multiplexing capabilities.
Lee
Howon
H
School of Electrical Engineering and Computer Science, Seoul National University, San 56-1, Shillim 9-dong, Gwanak-ku, Seoul 151-744, South Korea.
Kim
Junhoi
J
Kim
Hyoki
H
Kim
Jiyun
J
Kwon
Sunghoon
S
D001681:Biological Assay / Q000379:methods*; D003116:Color; D007700:Kinetics; D008280:Magnetics; D008863:Microspheres