title: Control of highly migratory cells by microstructured surface based on transient change in cell behavior.
abstract: Cell migration control techniques have been proposed for cells with relatively low migratory activity, based on static analyses performed with cells that attain a temporally homogenous state after being exposed to a cell guiding stimulus. To elucidate new functions of substrate topography, we investigated the transient change in the behavior of highly migratory cells coming from a flat surface to a grooved surface on a silicon substrate covered with SiO(2). A single line groove (1.5 μm in width, 20 μm in depth) and intersecting grooves (1.5 μm in width, 5 μm in spacing, 20 μm in depth) functioned as an effective cell repellent. In the case of wider grooves, a single line groove (4 μm in width; 20 μm in width) had no specified function. In contrast, intersecting grooves (4 μm in width, 5 μm in spacing) functioned as a trap for the cells. Our findings yield a new design concept of cell repelling and trapping surfaces which are applicable to cell guiding methods and single or multiple cell confinement on cell culture substrates, and thus may contribute to development of more advanced biomaterials.
authors: VCAD System Research Program, RIKEN, The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. hiromi-miyoshi@riken.jp
authors: Hiromi
authors: H
authors: Miyoshi
authors: Jungmyoung
authors: J
authors: Ju
authors: Sang Min
authors: SM
authors: Lee
authors: Dong Jin
authors: DJ
authors: Cho
authors: Jong Soo
authors: JS
authors: Ko
authors: Yutaka
authors: Y
authors: Yamagata
authors: Taiji
authors: T
authors: Adachi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D000078404:Epidermal Cells; D005399:Fishes; D013499:Surface Properties