Characterization of enterohemorrhagic Escherichia coli O157:H7 00B015: a Shiga toxin producing but virulence-attenuated isolate.
Enterohemorrhagic Escherichia coli (EHEC) causes a wide range of systematic diseases in human and animals in 2 main ways: (1) production of Shiga toxin (Stx) and (2) induction of actin polymerization characterized by attaching and effacing (A/E) lesions. Stx is commonly targeted in the development of drugs and vaccines to control EHEC infection for its indispensible contribution to EHEC pathogenesis. In this study, we isolated a Stx-producing EHEC O157:H7 isolate 00B015 and found that its ability to induce actin polymerization was impaired. In addition, it reduces pathogenicity and decreases mortality in mice. Our results report a Stx-producing but virulence-attenuated EHEC isolate 00B015 and suggest that the formation of actin polymerization may help Stx-induced pathogenesis and have a more important contribution in EHEC infections.
Wang
Haiguang
H
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China.
Gu
Jiang
J
Yu
Shu
S
Zhang
Weijun
W
Zhu
Yefei
Y
Zou
Quanming
Q
Zhu
Fengcai
F
Mao
Xuhu
X
D000199:Actins / Q000378:metabolism; D018830:Adhesins, Escherichia coli / Q000276:immunology; D000818:Animals; D004927:Escherichia coli Infections / Q000276:immunology / Q000473:pathology*; D019453:Escherichia coli O157 / Q000302:isolation & purification / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D055503:Protein Multimerization; D022621:Shiga Toxin / Q000235:genetics* / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology*
D000199:Actins; D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; D037521:Virulence Factors; D022621:Shiga Toxin