A genetically encoded photocaged Nepsilon-methyl-L-lysine.
A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
Wang
Yane-Shih
YS
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Wu
Bo
B
Wang
Zhiyong
Z
Huang
Ying
Y
Wan
Wei
W
Russell
William K
WK
Pai
Pei-Jing
PJ
Moe
Yin N
YN
Russell
David H
DH
Liu
Wenshe R
WR
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering; D021241:Spectrometry, Mass, Electrospray Ionization
D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine