Effects of fasting on hematologic and clinical chemical values in cynomolgus monkeys (Macaca fascicularis).
BACKGROUND
Fasting is an important pre-analytical factor that may affect clinical pathology parameters in toxicological and pharmacological studies. Little information is available on how fasting affects clinical pathology parameters in cynomolgus monkeys (Macaca fascicularis).The aim of this study was to evaluate the influence of fasting on clinical pathology parameters in healthy adult cynomolgus monkeys.


METHODS
Five female and six male cynomolgus monkeys were fasted for 0, 8, 16, and 24 hours. Changes in body weight (BW), core hematologic, and serum clinical chemical parameters were evaluated.


RESULTS
The BW significantly decreased after 24 hours of fasting. Significant decreases in red blood cell count, hemoglobin, hematocrit, and mean corpuscular volume and increases in mean cell hemoglobin and mean cell hemoglobin concentration were observed at 16 hours in males. In females, increasing the duration of fasting caused a significant time-dependent increase in platelets. Blood urea nitrogen showed significant decreases in female and male monkeys after fasting. Alkaline phosphatase increased in females after fasting. Aspartate transaminase significantly increased both in females and males at 8 hours. In females, alanine transaminase and lactate dehydrogenase significantly increased at 8 hours. Albumin significantly decreased in males 24 hours, but increased in females 16 hours after fasting. Serum glucose and triglyceride were not affected by fasting. Serum calcium decreased and inorganic phosphorus increased in males after fasting.


CONCLUSIONS
These results suggested that clinical pathology data would vary after fasting. The decision to feed or fast before blood collection for clinical pathology tests should be made based on careful consideration.
Zeng
X-C
XC
National Shanghai Center for Drug Safety Evaluation and Research, Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
Yang
C-M
CM
Pan
X-Y
XY
Yao
Y-S
YS
Pan
W
W
Zhou
C
C
Jiang
Z-R
ZR
Chang
Y
Y
Ma
J
J
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D001835:Body Weight; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D005215:Fasting / Q000502:physiology*; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008252:Macaca fascicularis / Q000097:blood*; D008297:Male; D010976:Platelet Count; D012737:Sex Factors; D013997:Time Factors
D006454:Hemoglobins; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase