[Mechanism study of beta-elemene derivative (ET) in NB4 cell on apoptosis induction].
OBJECTIVE
beta-Elemene is an active component of herb medicine Curcuma wenyujin, N-(beta-elemene-13-yl)tryptophan (ET) was synthesized to increase the activity. The antiproliferative and apoptotic effects of ET were investigated with NB4 cell.


METHODS
The mechanism of ET were studied with the technology of cell culture in vitro using MTT assay, flow cytometry and Western blot analysis.


RESULTS
ET inhibited cell growth and induced apoptosis in NB4 cell at concentrations less than 40 micromol x L(-1). The apoptosis induction of ET in NB4 cell was associated with the generation of hydrogen peroxide (H2O2) and activation of caspase-3. Catalase pretreatment blocked ET-induced apoptosis in NB4 cells.


CONCLUSIONS
ET induces apoptosis through generation of H2O2 in NB4 cell.
Yu
Zhiying
Z
Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry* / Q000494:pharmacology*
D012717:Sesquiterpenes; C445979:beta-elemene; D006861:Hydrogen Peroxide; D053148:Caspase 3