title: Acceleration of diabetic wound healing by low-dose radiation is associated with peripheral mobilization of bone marrow stem cells.
abstract: In this study we investigated the effect of repeated low-dose radiation exposure (75 mGy X ray) on skin wound healing in a rat model of diabetes. A skin wound was made on the backs of diabetic and age-matched control rats 60 days after diabetes was induced by a single injection of streptozotocin. Rats with skin wounds were immediately treated with whole-body radiation daily for 5, 10 or 15 days with a 2-day break every 5 days. Wound size was estimated 5, 10 and 15 days after wound formation. Repeated exposure of diabetic rats to low-dose radiation significantly accelerated skin wound healing compared to the nonirradiated diabetic group. Furthermore, low-dose radiation-induced improvement in healing was associated with increases in bone marrow and circulating CD31(+)/CD34(+) stem cells, vessel regeneration and cell proliferation in the wound tissue, and matrix metalloproteinase 2 and 9 expression. Therefore, we conclude that the acceleration of wound healing in diabetic rats by repeated exposure to low-dose radiation is associated with stimulation of bone marrow stem cell proliferation and peripheral mobilization.
authors: Department of Endocrinology, Jilin University, Changchun, 130021, China.
authors: Wei-Ying
authors: WY
authors: Guo
authors: Guan-Jun
authors: GJ
authors: Wang
authors: Ping
authors: P
authors: Wang
authors: Qiang
authors: Q
authors: Chen
authors: Yi
authors: Y
authors: Tan
authors: Lu
authors: L
authors: Cai
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000503:physiopathology / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000528:radiation effects; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000378:metabolism / Q000528:radiation effects; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9