Pectinase production by a Brazilian thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state and submerged fermentation.
Thermophilic organisms produce thermostable enzymes, which have a number of applications, justifying the interest in the isolation of new thermophilic strains and study of their enzymes. Thirty-four thermophilic and thermotolerant fungal strains were isolated from soil, organic compost, and an industrial waste pile based on their ability to grow at 45 degrees C and in a liquid medium containing pectin as the only carbon source. Among these fungi, 50% were identified at the genus level as Thermomyces, Aspergillus, Monascus, Chaetomium, Neosartoria, Scopulariopsis, and Thermomucor. All isolated strains produced pectinase during solid-state fermentation (SSF). The highest polygalacturonase (PG) activity was obtained in the culture medium of thermophilic strain N31 identified as Thermomucor indicae-seudaticae. Under SSF conditions on media containing a mixture of wheat bran and orange bagasse (1:1) at 70% of initial moisture, this fungus produced the maximum of 120 U/ml of exo-PG, while in submerged fermentation (SmF) it produced 13.6 U/ml. The crude PG from SmF was more thermostable than that from SSF and exhibited higher stability in acidic pH.
Martin
N
N
UNESP - Universidade Estadual Paulista, Laboratório de Bioquímica e Microbiologia Aplicada-IBILCE, São José do Rio Preto/SP, Brazil.
Guez
M A U
MA
Sette
L D
LD
Da Silva
R
R
Gomes
E
E
D001938:Brazil; D003470:Culture Media; D005285:Fermentation; D006358:Hot Temperature; D007218:Industrial Microbiology; D007220:Industrial Waste; D009090:Mucorales / Q000145:classification / Q000201:enzymology* / Q000254:growth & development* / Q000302:isolation & purification; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000096:biosynthesis*; D012988:Soil Microbiology; D013379:Substrate Specificity
D003470:Culture Media; D007220:Industrial Waste; D010368:Pectins; D011096:Polygalacturonase