Modification of polymer networks with bone sialoprotein promotes cell attachment and spreading.
Biomaterials used for tissue engineering scaffolds act as temporary substrates, on which cells deposit newly synthesized extracellular matrix. In cartilage tissue engineering, polycaprolactone/poly(2-hydroxyethyl methacrylate) (PCL/pHEMA) polymer blends have been used as scaffold materials, but their use in osseous tissue engineering has been more limited. The objective of this study was to evaluate modification of PCL/pHEMA surfaces with bone sialoprotein (BSP), an extracellular matrix protein important in regulating osseous tissue formation. Modification of surfaces with BSP significantly enhanced osteoblastic cell attachment and spreading, without compromising proliferation. Thus, BSP-immobilization may be a useful strategy for optimizing scaffolds for skeletal tissue engineering.
Chan
Wailen D
WD
Biomedical Engineering, The University of Western Ontario, London, Ontario, Canada.
Goldberg
Harvey A
HA
Hunter
Graeme K
GK
Dixon
S J
SJ
Rizkalla
Amin S
AS
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D021982:Cell-Matrix Junctions; D058574:Integrin-Binding Sialoprotein / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D058574:Integrin-Binding Sialoprotein; D008689:Methacrylates; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; C005044:hydroxyethyl methacrylate