Further evaluation of the quantitative chorioallantoic membrane test using trypan blue stain to predict the eye irritancy of chemicals.
The feasibility of using the chorioallantoic membrane (CAM) test using fertile hens' eggs as an alternative method to predict eye irritancy was examined for 12 test chemicals, comprising eight liquids, three powders and one emulsion. The judgement of the injurious effects of chemicals was carried out by a trypan blue staining method that was developed to overcome disadvantages arising from the lack of objectivity and quantitativeness in the original CAM test (Luepke's method). The amounts of pigment adsorbed by the CAM showed a good correlation with the scores obtained in the Draize eye irritation test. The results indicate that the trypan blue staining assay using CAM-TBS assay may be useful as an alternative to the in vivo eye irritation test. The next step will be to validate this method on an interlaboratory basis.
Hagino
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Shiseido Safety and Analytical Research Center, 1050 Nippa-cho, Kohoku-ku, Yokohama 223, Japan.
Itagaki
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H
Kato
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Kobayashi
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