Comparison of midturbinate flocked-swab specimens with nasopharyngeal aspirates for detection of respiratory viruses in children by the direct fluorescent antibody technique.
Paired nasopharyngeal aspirate (NPA) and midturbinate flocked-swab specimens from 153 children with respiratory symptoms were examined by the direct fluorescent antibody (DFA) technique. Seventy-four infants (49%) had a viral infection documented by DFA. The flocked-swab specimens had 93% sensitivity and 96.7% agreement with the NPA specimens, with a kappa coefficient of 93.4% (95% confidence interval, 0.877, 0.991).
Faden
Howard
H
Department of Pediatrics, Division of Infectious Diseases, State University of New York at Buffalo School of Medicine and Biomedical Sciences, Women and Children's Hospital, Buffalo, NY 14222, USA. hfaden@upa.chob.edu
D002675:Child, Preschool; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D007223:Infant; D009296:Nasal Cavity / Q000821:virology*; D009305:Nasopharynx / Q000821:virology*; D012140:Respiratory Tract Diseases / Q000821:virology*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis*; D014780:Viruses / Q000302:isolation & purification*