Genetic reassortment between high-virulent and low-virulent Dobrava-Belgrade virus strains.
The tri-segmented RNA genome of hantaviruses facilitates genetic reassortment by segment swapping when cells are co-infected with different virus strains. We found efficient in vitro reassortment between members of two different genetic lineages of the Dobrava-Belgrade virus species, the weakly virulent DOBV-Aa and highly virulent DOBV-Af. In all reassortants, S and L segments originated from the same parental strain, and only the M segment was exchanged. To identify functional differences between the parental strains DOBV-Aa and DOBV-Af in cell culture and to compare them with the reassortants, we studied elements of the innate immunity in virus-infected cells. The contrasting phenotypes of the parental viruses were maintained by the reassortants carrying the respective S and L segments of the parental virus and were not influenced by the origin of the M segment.
Kirsanovs
Sina
S
Institute of Medical Virology (Helmut Ruska Haus), Charité Medical School, Campus Charité Mitte, Charitéplatz 1, Berlin, Germany.
Klempa
Boris
B
Franke
Renate
R
Lee
Min-Hi
MH
Schönrich
Günther
G
Rang
Andreas
A
Kruger
Detlev H
DH
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D018778:Hantavirus Infections / Q000276:immunology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D011995:Recombination, Genetic; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
D014764:Viral Proteins