HDL 2 particles are associated with hyperglycaemia, lower PON1 activity and oxidative stress in type 2 diabetes mellitus patients.
OBJECTIVE
In this study we examined the relationship of oxidative stress and hyperglycaemia to antioxidative capacity of high-density cholesterol (HDL-C) particles in type 2 diabetes mellitus (DM).


METHODS
Oxidative stress status parameters (superoxide anion (O2(-)), superoxide dismutase (SOD) activity and paraoxonase (PON1) status were assessed in 114 patients with type 2 DM and 91 healthy subjects. HDL particle diameters were determined by non-denaturing polyacrylamide gradient (3-31%) gel electrophoresis.


RESULTS
Patients had significantly higher concentrations of oxidative stress parameter O2(-)(p<0.001) and antioxidative defence, SOD activity (p<0.001). Paraoxonase activity was significantly lower in diabetics (p<0.001). The PON1(192) phenotype distribution among study groups was not significantly different. HDL 3 phenotype was significantly prevalent among patients (p<0.001). Paraoxonase activity was significantly lower in patients with predominantly HDL 2 particles than in controls.


CONCLUSIONS
The results of our current study indicate that the diabetic HDL 2 phenotype is associated with hyperglycaemia, lower PON1 activity and elevated oxidative stress.
Stefanović
Aleksandra
A
Institute for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. alex@pharmacy.bg.ac.rs
Kotur-Stevuljević
Jelena
J
Spasić
Slavica
S
Vekić
Jelena
J
Zeljković
Aleksandra
A
Spasojević-Kalimanovska
Vesna
V
Jelić-Ivanović
Zorana
Z
D043303:Aryldialkylphosphatase / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000097:blood / Q000150:complications* / Q000201:enzymology* / Q000473:pathology; D053439:Lipoproteins, HDL2 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010316:Particle Size; D010641:Phenotype; D018709:Statistics, Nonparametric
D001786:Blood Glucose; D053439:Lipoproteins, HDL2; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human