A proteomic approach towards the identification of the matrix protein content of the two types of microbodies in Neurospora crassa.
Microbodies (peroxisomes) comprise a class of organelles with a similar biogenesis but remarkable biochemical heterogeneity. Here, we purified the two distinct microbody family members of filamentous fungi, glyoxysomes and Woronin bodies, from Neurospora crassa and analyzed their protein content by HPLC/ESI-MS/MS. In the purified Woronin bodies, we unambiguously identified only hexagonal 1 (HEX1), suggesting that the matrix is probably exclusively filled with the HEX1 hexagonal crystal. The proteomic analysis of highly purified glyoxysomes allowed the identification of 191 proteins. Among them were 16 proteins with a peroxisomal targeting signal type 1 (PTS1) and three with a PTS2. The collection also contained the previously described N. crassa glyoxysomal matrix proteins FOX2 and ICL1 that lack a typical PTS. Three PTS1 proteins were identified that likely represent the long sought glyoxysomal acyl-CoA dehydrogenases of filamentous fungi. Two of them were demonstrated by subcellular localization studies to be indeed glyoxysomal. Furthermore, two PTS proteins were identified that are suggested to be involved in the detoxification of nitroalkanes. Since the glyoxysomal localization was experimentally demonstrated for one of these enzymes, a new biochemical reaction is expected to be associated with microbody function.
Managadze
David
D
Department of Systems Biochemistry, Institute of Physiological Chemistry, Ruhr-University of Bochum, Bochum, Germany.
Würtz
Christian
C
Wiese
Sebastian
S
Meyer
Helmut E
HE
Niehaus
Gerd
G
Erdmann
Ralf
R
Warscheid
Bettina
B
Rottensteiner
Hanspeter
H
D005656:Fungal Proteins / Q000032:analysis* / Q000302:isolation & purification; D008830:Microbodies / Q000737:chemistry*; D009492:Neurospora crassa / Q000737:chemistry*; D040901:Proteomics
D005656:Fungal Proteins