Effect of NEFA and glucose levels on CPT-I mRNA expression and translation in cultured bovine hepatocytes.
The objective of this study was to determine the effects of NEFA and glucose on carnitine palmitoyltransferase-I (CPT-I) mRNA expression in cultured bovine hepatocytes using real-time reverse transcription polymerase chain reaction and ELISA methods. The results indicated that CPT-I transcription increased gradually, but that CPT-I translation was not significantly changed, with glucose concentrations ranging from 0 to 3.0 mmol/L (P<0.01). Furthermore CPT-I transcription and translation were enhanced significantly when the NEFA concentrations increased from 0 to 1.2 mmol/L and decreased significantly when the NEFA concentrations increased from 1.2 to 4.8 mmol/L (P<0.01). A high concentration NEFA was found to reduce fatty acid oxidation, potentially explaining the development from NEB to ketosis in dairy cows.
Xu
Chuang
C
Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University, PR China.
Wang
Zhe
Z
Zhang
Ri-He
RH
Zhang
Hong-You
HY
Fu
Shi-Xin
SX
Xia
Cheng
C
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase; D005947:Glucose