title: A genetically encoded photocaged Nepsilon-methyl-L-lysine.
abstract: A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
authors: Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
authors: Yane-Shih
authors: YS
authors: Wang
authors: Bo
authors: B
authors: Wu
authors: Zhiyong
authors: Z
authors: Wang
authors: Ying
authors: Y
authors: Huang
authors: Wei
authors: W
authors: Wan
authors: William K
authors: WK
authors: Russell
authors: Pei-Jing
authors: PJ
authors: Pai
authors: Yin N
authors: YN
authors: Moe
authors: David H
authors: DH
authors: Russell
authors: Wenshe R
authors: WR
authors: Liu
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine