title: SAXS and X-ray crystallography suggest an unfolding model for the GDP/GTP conformational switch of the small GTPase Arf6.
abstract: The small GTPases Arf1 and Arf6 have nonoverlapping functions in cellular traffic despite their very high sequence and structural resemblance. Notably, the exquisite isoform specificity of their guanine nucleotide exchange factors and their distinctive sensitivity to the drug brefeldin A cannot be explained by any straightforward structural model. Here we integrated structural and spectroscopic methods to address this issue using Δ13Arf6-GDP, a truncated mutant that mimics membrane-bound Arf6-GDP. The crystal structure of Δ13Arf6-GDP reveals an unprecedented unfolding of the GTPase core β-strands, which is fully accounted for by small-angle X-ray scattering data in solution and by ab initio three-dimensional envelope calculation. NMR chemical shifts identify this structural disorder in Δ13Arf6-GDP, but not in the closely related Δ17Arf1-GDP, which is consistent with their comparative thermodynamic and hydrodynamic analyses. Taken together, these experiments suggest an unfolding model for the nucleotide switch of Arf6 and shed new light on its biochemical differences with Arf1.
authors: Laboratoire d'Enzymologie et Biochimie Structurales, Centre de Recherche CNRS de Gif-Sur-Yvette, Gif-sur-Yvette, France. biou@lebs.cnrs-gif.fr
authors: Valérie
authors: V
authors: Biou
authors: Kaheina
authors: K
authors: Aizel
authors: Pierre
authors: P
authors: Roblin
authors: Aurélien
authors: A
authors: Thureau
authors: Eric
authors: E
authors: Jacquet
authors: Sebastian
authors: S
authors: Hansson
authors: Bernard
authors: B
authors: Guibert
authors: Eric
authors: E
authors: Guittet
authors: Carine
authors: C
authors: van Heijenoort
authors: Mahel
authors: M
authors: Zeghouf
authors: Javier
authors: J
authors: Perez
authors: Jacqueline
authors: J
authors: Cherfils
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006153:Guanosine Diphosphate / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry*; D006801:Humans; D011487:Protein Conformation; D017510:Protein Folding; D053838:Scattering, Small Angle; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D000089982:ADP-Ribosylation Factor 6; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human