title: [Erythropoietin gene-modified conditioned medium of human mesenchymal cells promotes hematopoietic development from human embryonic stem cells].
abstract: The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells.
authors: Laboratory of Stem Cell and Regenerative Medicine, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
authors: Chao
authors: C
authors: Yang
authors: Lei
authors: L
authors: Ji
authors: Wen
authors: W
authors: Yue
authors: Ruo-Yong
authors: RY
authors: Wang
authors: Yan-Hua
authors: YH
authors: Li
authors: Jia-Fei
authors: JF
authors: Xi
authors: Xiao-Yan
authors: XY
authors: Xie
authors: Li-Juan
authors: LJ
authors: He
authors: Xue
authors: X
authors: Nan
authors: Xue-Tao
authors: XT
authors: Pei
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D004921:Erythropoietin / Q000235:genetics / Q000494:pharmacology*; D006413:Hematopoietic System; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D030781:Organisms, Genetically Modified
chemical_list: D017077:Culture Media, Conditioned; D004921:Erythropoietin