[MAST2-like protein kinase from grape vine Vitis vinifera: cloning of catalytic domain cDNA].
The aim of our work is the identification of protein kinases phosphorylating microtubule proteins in plant cells. Using bioinformatic approach, we found genes of putative homologues of microtubule-associated mammalian protein kinase MAST2 in higher plant genomes. The gene of closest MAST2 homologue, putative protein, named GMLK (Grape MAST2-Like Kinase, A7NTE9_VITVI), was found in grape Vitis vinifera. We report here the cloning of cDNA of GMLK (A7NTE9) from Pinot Noir grape vine leaves.
Briantseva
S A
SA
Gavriushina
E S
ES
Emets
A I
AI
Karpov
P A
PA
Blium
Ia B
IaB
Drygin
Iu F
IuF
Nadezhdina
E S
ES
D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008870:Microtubules / Q000201:enzymology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000201:enzymology / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D016415:Sequence Alignment; D027843:Vitis / Q000201:enzymology* / Q000648:ultrastructure
D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; C503880:MAST2 protein, human; D017346:Protein Serine-Threonine Kinases