Increases in airway eosinophilia and a th1 cytokine during the chronic asymptomatic phase of asthma.
BACKGROUND
Studies using allergen challenge models have suggested Th2 cytokines promote airway inflammation in asthma. We assessed mediators of airway inflammation during the chronic asymptomatic phase of asthma.


METHODS
Nine non-atopic asthma (NAA) patients, 19 atopic asthma (AA) patients, 20 atopic controls (AC), and 38 normal controls (NC) underwent sputum induction while asymptomatic. Sputum total cell counts and differentials were determined; levels of cytokines IL-4, IL-5, IL-13, GM-CSF, and IFN-gamma, and chemokines eotaxin (CCL11) and RANTES (CCL5) were measured by ELISA; and levels of eosinophil-derived neurotoxin (EDN) were measured by radioimmunoassay.


RESULTS
NAA patients showed higher % eosinophils and total eosinophils compared to AA. NAA and AA patients showed higher IFN-gamma and EDN levels compared to AC and NC, with no differences in IL-4, IL-5, or IL-13 levels among the four groups. GM-CSF levels were higher in AA patients compared to AC or NC. In NAA, AA, and AC patients, % eosinophils and EDN levels correlated positively with IFN-gamma, GM-CSF, eotaxin, and RANTES, but not with IL-5 levels.


CONCLUSIONS
Baseline airway inflammation of intrinsic and extrinsic asthma is characterized by eosinophilic inflammation and the Th1 cytokine, IFN-gamma. GM-CSF, instead of IL-5, and chemokines may coordinate airway eosinophilia during the chronic asymptomatic phase of asthma.
Kim
Chang Keun
CK
Asthma and Allergy Center, Inje University Sanggye Paik Hospital, Seoul, Korea.
Choi
Jungi
J
Callaway
Zak
Z
Iijima
Koji
K
Volcheck
Gerald
G
Kita
Hirohito
H
D000328:Adult; D000368:Aged; D001249:Asthma / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D016022:Case-Control Studies; D054413:Chemokine CCL11 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004802:Eosinophilia / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D004804:Eosinophils / Q000378:metabolism*; D005260:Female; D005541:Forced Expiratory Volume / Q000502:physiology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013183:Sputum / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
D054413:Chemokine CCL11; D018946:Chemokine CCL5; D016207:Cytokines; D007378:Interleukins; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor