Enzymatic glycosylations on arrays.
The enzymatic glycosylation of microarrays is a relatively young field in glycoscience. Platforms developed from other array technologies (e.g., proteins and nucleic acids) were successfully adopted in several proof-of-principle studies as a high-throughput tool for the generation of more complex carbohydrate structures using carbohydrate-processing enzymes. These arrays and the developed on-chip enzymatic glycosylation methodologies are reviewed in this article.
Voglmeir
Josef
J
Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7ND, UK.
Sardzík
Robert
R
Weissenborn
Martin J
MJ
Flitsch
Sabine L
SL
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D004798:Enzymes / Q000378:metabolism*; D054794:Glycomics / Q000295:instrumentation / Q000379:methods*; D006031:Glycosylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D000906:Antibodies; D002241:Carbohydrates; D004798:Enzymes