title: Biodegradation of 2,4-dichlorophenol in a fluidized bed reactor with immobilized Phanerochaete chrysosporium.
abstract: The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)⁻¹ could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)⁻¹, and the saturation constant was 26.045 mg L⁻¹.
authors: Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, P.R. China.
authors: Xiao-ming
authors: XM
authors: Li
authors: Qi
authors: Q
authors: Yang
authors: Ying
authors: Y
authors: Zhang
authors: Wei
authors: W
authors: Zheng
authors: Xiu
authors: X
authors: Yue
authors: Dong-bo
authors: DB
authors: Wang
authors: Guang-ming
authors: GM
authors: Zeng
mesh_terms: D019149:Bioreactors; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism*; D002733:Chlorophenols / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D005656:Fungal Proteins / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D002733:Chlorophenols; D003470:Culture Media; D005656:Fungal Proteins; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C004762:2,4-dichlorophenol