Activity-based protein profiling for biochemical pathway discovery in cancer.
Large-scale profiling methods have uncovered numerous gene and protein expression changes that correlate with tumorigenesis. However, determining the relevance of these expression changes and which biochemical pathways they affect has been hindered by our incomplete understanding of the proteome and its myriad functions and modes of regulation. Activity-based profiling platforms enable both the discovery of cancer-relevant enzymes and selective pharmacological probes to perturb and characterize these proteins in tumour cells. When integrated with other large-scale profiling methods, activity-based proteomics can provide insight into the metabolic and signalling pathways that support cancer pathogenesis and illuminate new strategies for disease diagnosis and treatment.
Nomura
Daniel K
DK
The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037, USA.
Dix
Melissa M
MM
Cravatt
Benjamin F
BF
D000818:Animals; D006801:Humans; D055432:Metabolomics; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000201:enzymology* / Q000628:therapy; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D013379:Substrate Specificity