title: Pancreatic function in carboxyl-ester lipase knockout mice.
abstract: OBJECTIVE
  CEL-MODY is a monogenic form of diabetes and exocrine pancreatic insufficiency due to mutations in the carboxyl-ester lipase (CEL) gene. We aimed to investigate endocrine and exocrine pancreatic function in CEL knockout mice (CELKO).
  
  
  METHODS
  A knockout mouse model with global targeted deletion of CEL was investigated physiologically and histopathologically, and compared to littermate control CEL+/+ mice at 7 and 12 months on normal chow and high-fat diets (HFD), i.e. 42 and 60% fat by calories.
  
  
  RESULTS
  CELKO+/+ and -/- mice showed normal growth and development and normal glucose metabolism on a chow diet. Female CEL-/- mice on 60% HFD, on the other hand, had increased random blood glucose compared to littermate controls (p = 0.02), and this was accompanied by a reduction in glucose tolerance that did not reach statistical significance. In these mice there was also islet hyperplasia, however, α- and β-islet cells appeared morphologically normal and pancreatic exocrine function was also normal.
  
  
  CONCLUSIONS
  Although we observed mild glucose intolerance in female mice with whole-body knockout of CEL, the full phenotype of human CEL-MODY was not reproduced, suggesting that the pathogenic mechanisms involved are more complex than a simple loss of CEL function. and IAP.
authors: Department of Pediatrics, Haukeland University Hospital, Bergen, Norway.
authors: Mette
authors: M
authors: Vesterhus
authors: Helge
authors: H
authors: Raeder
authors: Amarnath J
authors: AJ
authors: Kurpad
authors: Dan
authors: D
authors: Kawamori
authors: Anders
authors: A
authors: Molven
authors: Rohit N
authors: RN
authors: Kulkarni
authors: C Ronald
authors: CR
authors: Kahn
authors: Pål Rasmus
authors: PR
authors: Njølstad
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D050416:Glucagon-Secreting Cells / Q000166:cytology / Q000378:metabolism; D005951:Glucose Tolerance Test; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D007408:Intestinal Absorption / Q000235:genetics; D007515:Islets of Langerhans / Q000166:cytology / Q000201:enzymology*; D008049:Lipase / Q000502:physiology*; D008136:Longevity / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046790:Pancreas, Exocrine / Q000166:cytology / Q000201:enzymology*; D015430:Weight Gain / Q000235:genetics
chemical_list: D001786:Blood Glucose; D008049:Lipase