title: Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells.
abstract: VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task. In the present paper two fragments of VP2 have been expressed successfully into the medium of transiently transfected mammalian cells through a fusion peptides strategy. The crude conditioned medium containing the secreted peptide could be employed for immunodiagnostic assay development or vaccine purposes.
authors: Dipartimento di Salute Animale, Facoltà di Medicina Veterinaria, Università di Parma, via del Taglio 10, 43100 Parma, Italy.
authors: A
authors: A
authors: Capocefalo
authors: V
authors: V
authors: Franceschi
authors: P P
authors: PP
authors: Mertens
authors: J
authors: J
authors: Castillo-Olivares
authors: S
authors: S
authors: Cavirani
authors: E
authors: E
authors: Di Lonardo
authors: Z
authors: Z
authors: Leni
authors: G
authors: G
authors: Donofrio
mesh_terms: D001820:Bluetongue virus / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D003470:Culture Media; D011993:Recombinant Fusion Proteins; C442520:VP2 protein, Bluetongue virus