title: Simultaneous determination of albendazole and praziquantel by second derivative spectrophotometry and multivariated calibration methods in veterinary pharmaceutical formulation.
abstract: The simultaneous determination of albendazole (ABZ) and praziquantel (PZQ) was performed by different mathematical approaches: second derivative spectrophotometry (SDS), classical least squares, regression of partial least squares and principal components regression based on spectral data of drugs dissolved in methanol-hydrochloric acid solution. The detection limits for multivariate calibrations were determined by creating a surrogate variable signal. SDS presented the best analytical features. The recoveries of ABZ and PZQ from the synthetic samples were near to 100 +/- 5%. The methods were applied in veterinary pharmaceutical formulation whose mass ratio ABZ:PZQ is 10:1; the results obtained were according to nominal content.
authors: Department of Analytical and Inorganic Chemistry, Faculty of Chemical Sciences, University of Concepción, Chile. cesarsoto@udec.cl
authors: César
authors: C
authors: Soto
authors: David
authors: D
authors: Contreras
authors: Sandra
authors: S
authors: Orellana
authors: Jorge
authors: J
authors: Yañez
authors: M Inés
authors: MI
authors: Toral
mesh_terms: D015766:Albendazole / Q000032:analysis*; D000818:Animals; D006851:Hydrochloric Acid; D016018:Least-Squares Analysis; D057230:Limit of Detection; D000432:Methanol; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011223:Praziquantel / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations; D019155:Veterinary Drugs; D011223:Praziquantel; D015766:Albendazole; D006851:Hydrochloric Acid; D000432:Methanol