[Expression of Copper-Transporting P-Type Adenosine Triphosphatase (ATP7B) Correlates with Cisplatin-Resistance in Human Lung Adenocarcinoma Cell Line A549.].
BACKGROUND
Cisplatin is the basic chemotherapy agent of lung carcinoma, and cisplatin-resistance mostly leads to the failing of chemotherapy in the patients. The aim of this study is to investigate the relationship between the expression of copper-transporting P-type adenosine triphosphatase (ATP7B) and cisplatinresistance in different cisplatin-resistant A549 cells.


METHODS
Three differently cisplatin-resistant A549 sublines (A549/DDP0.5, A549/DDP1.0, A549/DDP2.0) were established from their parental human lung adenocarcinoma cell line A549 which was sensitive to cisplatin, by gradually increasing concentration of cisplatin. The resistance indexes of all the sublines were checked by MTT method. The levels of ATP7B mRNA and protein were measured by RT-PCR and Western Blot respectively in all the cell lines.


RESULTS
The resistance indexes of the three cisplatin-resistant sublines were 1.7, 3.2 and 5.2 respectively (P <0.001), and the levels of ATP7B mRNA were 1.6, 4.9 and 10.1 folds compared to the parental A549 cells (P <0.001). Meanwhile, expression of ATP7B protein was enhanced in the three cisplatin-resistant sulines corresponding to their cisplatin-resistance.


CONCLUSIONS
Expression of ATP7B correlates with cisplatin-resistance in A549 cells, and maybe ATP7B contributes to acquisition of cisplatin-resistance.
Gao
Guizhou
G
Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Wang
Jianjun
J
Shi
Si'en
S