blaROB-1 presence on pB1000 in Haemophilus influenzae is widespread, and variable cefaclor resistance is associated with altered penicillin-binding proteins.
Plasmid pB1000 is a small replicon recently identified as bearing bla(ROB-1) in animal and human Pasteurellaceae in Spain. We identified pB1000 in 11 bla(ROB-1)-positive Australian and North American Haemophilus influenzae isolates, suggesting a wider role for pB1000 in disseminating bla(ROB-1). Native H. influenzae conjugative elements can mobilize plasmids similar to pB1000 at a low frequency of 10(-8), and this might account for the infrequency of bla(ROB-1) compared to the rate of occurrence of bla(TEM-1). Altered penicillin-binding protein 3 was associated with an increased cefaclor MIC in 3 isolates.
Tristram
Stephen G
SG
School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia. Stephen.Tristram@utas.edu.au
Littlejohn
Rachael
R
Bradbury
Richard S
RS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002433:Cefaclor / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics; D006193:Haemophilus influenzae / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D012093:Replicon / Q000235:genetics*
D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D002433:Cefaclor