title: Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice.
abstract: This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.
authors: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
authors: Eun Hee
authors: EH
authors: Ahn
authors: Dae Won
authors: DW
authors: Kim
authors: Hye Won
authors: HW
authors: Kang
authors: Min Jae
authors: MJ
authors: Shin
authors: Moo Ho
authors: MH
authors: Won
authors: Joon
authors: J
authors: Kim
authors: Dong Joon
authors: DJ
authors: Kim
authors: Oh-Shin
authors: OS
authors: Kwon
authors: Tae-Cheon
authors: TC
authors: Kang
authors: Kyu Hyung
authors: KH
authors: Han
authors: Jinseu
authors: J
authors: Park
authors: Won Sik
authors: WS
authors: Eum
authors: Soo Young
authors: SY
authors: Choi
mesh_terms: D000818:Animals; D003872:Dermatitis / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D004487:Edema / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D005822:Genetic Vectors / Q000627:therapeutic use; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism / Q000502:physiology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010447:Peptide Hydrolases / Q000235:genetics* / Q000627:therapeutic use; D010940:Plant Proteins / Q000235:genetics* / Q000627:therapeutic use; D012269:Ribosomal Proteins / Q000235:genetics* / Q000627:therapeutic use; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D018836:Inflammation Mediators; D010940:Plant Proteins; D012269:Ribosomal Proteins; C082041:YRPS3 protein, Petunia hybrida; C044152:ribosomal protein S3; D010447:Peptide Hydrolases; C058973:peptidase-1 (Pleurodeles); D013755:Tetradecanoylphorbol Acetate