Synthesis of linear polyamines with different amine spacings and their ability to form dsDNA/siRNA complexes suitable for transfection.
In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to phosphorus (N/P) ratios using small-angle neutron scattering (SANS) and atomic force microscopy (AFM) techniques. AFM images showed that while linear poly(ethylene imine) (PEI)/DNA complex results in bigger spherical aggregates, poly(propylene imine)s forms torroid and cigar shaped structures upon complexation with DNA. The poly(butylene imine)s (LPBI)s form compact and soluble DNA complexes with a radii range of R(g) = 15-30 nm. Among the studied linear polyamines, the LPBIs did show the best transfection efficiency.
Fischer
Wiebke
W
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
Brissault
Blandine
B
Prévost
Sylvain
S
Kopaczynska
Marta
M
Andreou
Ioanna
I
Janosch
Andrea
A
Gradzielski
Michael
M
Haag
Rainer
R
D004247:DNA / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D033363:Neutron Diffraction; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000627:therapeutic use; D034741:RNA, Small Interfering / Q000737:chemistry*; D053838:Scattering, Small Angle; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods*
D046911:Macromolecular Substances; D011073:Polyamines; D034741:RNA, Small Interfering; D004247:DNA