[Detection of molecular epidemiology of Mycobacterium gordonae isolates].
OBJECTIVE
To analyze the molecular epidemiology of Mycobacterium gordonae strains from patients and environments in the hospital.


METHODS
A total of 46 clinical strains were obtained from patients registered at the NHO Kinki-chuo Chest Medical Center and 3 strains from hospital environments.


METHODS
By using genetic data from the 16S rRNA gene and hsp65PRA, pulsed-field gel electrophoresis (PFGE) assessment of their intraspecies variability and epidemiology was carried out.


RESULTS
Strains from six patients and environmental cultures exhibited the different genotypes of 16S rRNA gene sequencing and the hsp65PRA type. The PFGE analysis suggested no pseudo-outbreak and showed a polyclonal infection in one patient.


CONCLUSIONS
These findings suggest that we should maintain effective surveillance of environments in the hospital and continuously perform molecular epidemiological investigations for infection control of M. gordonae.
Yoshida
Shiomi
S
Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan. dustin@kch.hosp.go.jp
Suzuki
Katsuhiro
K
Iwamoto
Tomotada
T
Tsuyuguchi
Kazunari
K
Tomita
Motohisa
M
Okada
Masaji
M
Sakatani
Mitsunori
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000032:analysis; D018834:Chaperonin 60 / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology*; D009170:Nontuberculous Mycobacteria / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000032:analysis
D001426:Bacterial Proteins; D018834:Chaperonin 60; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium