False negative results in high viremia parvovirus B19-samples tested with real-time PCR.
Extremely high viremia is observed during some viruses infection, especialy in immunocompromised patients. False negative results of Parvovirus B 19 DNA tests performed with real-time PCR in high viremic samples are reported. The way of fluorescence diagrams analysis and algorithm of positive result confirmation to exclude such phenomenon are proposed.
Grabarczyk
Piotr
P
Department of Immunohaematology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland. pgrabarczyk@ihit.waw.pl
Kalińska
Aleksandra
A
Sulkowska
Ewa
E
Brojer
Ewa
E
D005188:False Negative Reactions; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014766:Viremia / Q000175:diagnosis* / Q000821:virology