Influence of chirality using Mn(III) salen complexes on DNA binding and antioxidant activity.
Chiral Mn(iii) salen complexes S-1, R-1, S-2, R-2, S-3 and R-3 derived from the respective chiral salen ligands, viz., (1S,2S)-N,N'-bis-[3-tert-butyl-5-chloromethyl-salicylidine]-1,2-cyclohexanediamine S-1'/(1R,2R)-N,N'-bis-[3-tert-butyl-5-chloromethyl-salicylidine]-1,2-cyclohexanediamine R-1'/(1S,2S)-N,N'-bis-[3-tert-butyl-5-N,N'N'triethylaminomethyl-salicylidine]-1,2-cyclohexanediamine dichloride S-2'/(1R,2R)-N,N'-bis-[3-tert-butyl-5-N,N'N'triethylaminomethyl-salicylidine]-1,2-cyclohexanediamine dichloride R-2'/(1S,2S)-N,N'-bis-[3,5-di-tert-butylsalicylidene]-1,2-cyclohexanediamine S-3' and (1R,2R)-N,N'-bis-[3,5-di-tert-butyl-salicylidene]-1,2-cyclohexanediamine R-3', were synthesized. Characterization of the complexes was done by microanalysis, IR, LC-MS, UV-vis. and circular dichroism (CD) spectroscopy. Binding of these complexes with calf thymus DNA (CT-DNA) was studied by absorption spectroscopy, competitive binding study, viscosity measurements, circular dichroism measurements, thermal denaturation study and observation of their different antioxidant activities. Among all the complexes used, the best result in terms of binding constant (intercalative) (130.4 x 10(4)) was achieved with the complex S-1 by spectroscopic titration. The complex S-1 showed strong antioxidant activity as well.
Khan
Noor-Ul H
NU
Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute (CSMCRI), Council of Scientific & Industrial Research (CSIR), G. B. Marg, Bhavnagar-364 021, Gujarat, India. khan251293@yahoo.in
Pandya
Nirali
N
Kumar
Manoj
M
Bera
Prasanta Kumar
PK
Kureshy
Rukhsana I
RI
Abdi
Sayed H R
SH
Bajaj
Hari C
HC
D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008024:Ligands; D017895:Manganese Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D013237:Stereoisomerism; D013481:Superoxides / Q000378:metabolism; D014783:Viscosity / Q000187:drug effects
D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D008024:Ligands; D017895:Manganese Compounds; D010851:Picrates; D013481:Superoxides; D004247:DNA; C427203:calf thymus DNA; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl