title: Increased calcium deposits and decreased Ca2+ -ATPase in erythrocytes of ascitic broiler chickens.
abstract: The decrease of erythrocyte deformability may be one of the predisposing factors for pulmonary hypertension and ascites in broiler chickens. In mammals, the cytoplasmic calcium is a major regulator of erythrocyte deformability. In this study, the erythrocyte deformability was measured, and the precise locations of Ca2+ and Ca2+ -ATPase in the erythrocytes were investigated in chickens with ascites syndrome induced by low ambient temperature. The results showed that ascitic broilers had higher filtration index of erythrocyte compared with control groups, indicating a decrease in erythrocyte deformability in ascitic broilers. The more calcium deposits were observed in the erythrocytes of ascitic broilers compared with those of the age-matched control birds. The Ca2+ -ATPase reactive grains were significantly decreased on the erythrocyte membranes of ascitic broilers. Our data suggest that accumulation of intracellular calcium and inhibition of Ca2+ -ATPase might be important factors for the reduced deformability of the erythrocytes of ascitic broilers.
authors: Department of Animal Physiopathology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
authors: Kai
authors: K
authors: Li
authors: Lihong
authors: L
authors: Zhao
authors: Guangrui
authors: G
authors: Geng
authors: Liqin
authors: L
authors: Ma
authors: Shishan
authors: S
authors: Dong
authors: Tong
authors: T
authors: Xu
authors: Jianlin
authors: J
authors: Wang
authors: Huiyu
authors: H
authors: Wang
authors: Yong
authors: Y
authors: Tian
authors: Jian
authors: J
authors: Qiao
mesh_terms: D000818:Animals; D001201:Ascites / Q000097:blood / Q000201:enzymology / Q000662:veterinary*; D002118:Calcium / Q000097:blood*; D000252:Calcium-Transporting ATPases / Q000097:blood*; D002645:Chickens / Q000097:blood*; D004910:Erythrocyte Membrane / Q000000981:diagnostic imaging / Q000201:enzymology; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology* / Q000648:ultrastructure; D006400:Hematocrit / Q000662:veterinary; D011201:Poultry Diseases / Q000097:blood* / Q000201:enzymology; D014463:Ultrasonography
chemical_list: D000252:Calcium-Transporting ATPases; D002118:Calcium