title: [Killing effect of dendritic cell vaccine transfected by recombinant adeno-associated virus with hAFP gene fragment on hepatocellular carcinoma cells in vitro].
abstract: OBJECTIVE
  Dendritic cell vaccines are one of the important active immunotherapies for neoplasms. The aim of this study was to observe the killing effect of specific cytotoxic T lymphocytes (CTL) on liver carcinoma HepG2 and SMMC-7721 cells in vitro. The CTL was induced by human peripheral blood mononuclear cells-originated dendritic cells (DC) transfected by recombinant adeno-associated virus (rAAV) with hAFP gene fragment (137-145).
  
  
  METHODS
  Immature DCs were generated from peripheral blood mononuclear cells of healthy volunteers and then transfected by rAAV with AFP gene fragment. The CTL was thereafter induced. The activities of DC and CTL were measured and the killing effect of the CTL on HepG2 cells was detected using M1Tr assay.
  
  
  RESULTS
  The mature DC, transfected or not, highly expressed CD40, CD86 and IL-12. IFN-gamma was highly expressed in the CTL. The DC-induced CTL could effectively recognize and destroy the HepG2 and SMMC-7721 cells.
  
  
  CONCLUSIONS
  DC transfected by rAAV can stimulate the proliferation and differentiation of lymphocytes and also induce the proliferation of CTL, and their own phenotypes are not significantly altered. The DC vaccine can be effectively used as an adjuvant immunotherapy for patients with hepatocellular carcinoma.
authors: Department of Oncology, Zhongshan Hospital in Hubei Province, Wuhan 430030, China. sunjian.hai@163.com
authors: Jian-hai
authors: JH
authors: Sun
authors: Yan-ling
authors: YL
authors: Ma
authors: Ming-e
authors: ME
authors: Peng
authors: Ai-gui
authors: AG
authors: Bie
authors: Li
authors: L
authors: Li
authors: Li
authors: L
authors: Liu
mesh_terms: D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D019496:Cancer Vaccines / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology*; D010446:Peptide Fragments / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D051940:B7-2 Antigen; D019013:CD40 Antigens; D019496:Cancer Vaccines; D010446:Peptide Fragments; D000509:alpha-Fetoproteins; D018664:Interleukin-12; D007371:Interferon-gamma