Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells.
Angiogenesis is critical to a wide range of physiological and pathological processes. Scutellarin, a major flavonoid of a Chinese herbal medicine Erigeron breviscapus (Vant.) Hand. Mazz. has been shown to offer beneficial effects on cardiovascular and cerebrovascular functions. However, scutellarin's effects on angiogenesis and underlying mechanisms are not fully elucidated. Here, we studied angiogenic effects of scutellarin on human umbilical vein endothelial cells (HUVECs) in vitro. Scutellarin was found by MTT assay to induce proliferation of HUVECs. In scutellarin-treated HUVECs, a dramatic increase in migration was measured by wound healing assay; Transwell chamber assay found significantly more invading cells in scutellarin-treated groups. Scutellarin also promoted capillary-like tube formation in HUVECs on Matrigel, and significantly upregulated platelet endothelial cell adhesion molecule-1 at both mRNA and protein levels. Scutellarin's angiogenic mechanism was investigated in vitro by measuring expression of angiogenic factors associated with cell migration and invasion. Scutellarin strongly induced MMP-2 activation and mRNA expression in cultured HUVECs in a concentration-dependent manner. Taken together, these results suggest that scutellarin promotes angiogenesis and may form a basis for angiogenic therapy.
Gao
Zhong-Xiu-Zi
ZX
Department of Anatomy, Basic Medical Science College, Harbin Medical University, Harbin, China.
Huang
Da-Yong
DY
Li
Hai-Xia
HX
Zhang
Li-Na
LN
Lv
Yan-Hong
YH
Cui
Hai-Dong
HD
Zheng
Jin-Hua
JH
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005965:Glucuronates / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects
D043925:Angiogenesis Inducing Agents; D005965:Glucuronates; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019717:Tissue Inhibitor of Metalloproteinase-3; C484876:scutellarin; D047310:Apigenin; D020778:Matrix Metalloproteinase 2