A systems approach to analyze transcription factors in mammalian cells.
Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.
Soler
Eric
E
Department of Cell Biology, Erasmus Medical Center, Dr. Molewaterplein 50, Rotterdam, The Netherlands. e.soler@erasmusmc.nl
Andrieu-Soler
Charlotte
C
Boer
Ernie de
Ed
Bryne
Jan Christian
JC
Thongjuea
Supat
S
Rijkers
Erikjan
E
Demmers
Jeroen
J
van IJcken
Wilfred
W
Grosveld
Frank
F
D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D049490:Systems Biology / Q000379:methods*; D014157:Transcription Factors / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology
D014157:Transcription Factors