Deletion of sua gene reduces the ability of Streptococcus uberis to adhere to and internalize into bovine mammary epithelial cells.
To elucidate the role of Streptococcus uberis adhesion molecule (SUAM) in the pathogenesis of S. uberis mastitis, sua deletion in S. uberis UT888 was achieved by homologous recombination using a thermosensitive plasmid. The deletion mutant was analyzed for sua deletion by PCR, southern blot and DNA sequencing, and was designated Δsua S. uberis UT888. As compared to the isogenic parent strain, Δsua S. uberis UT888 did not produce SUAM based on SDS-PAGE gel and western blot. Deletion of sua and lack of expression of SUAM by Δsua S. uberis UT888 markedly reduced the ability of the sua gene deletion mutant of S. uberis to adhere to and internalize into mammary epithelial cells. These results confirm the central role of SUAM in adherence to and internalization of S. uberis into host cells.
Chen
Xueyan
X
Department of Animal Science, The University of Tennessee, Knoxville, TN 37996, USA.
Dego
Oudessa Kerro
OK
Almeida
Raul A
RA
Fuller
Troy E
TE
Luther
Douglas A
DA
Oliver
Stephen P
SP
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D004847:Epithelial Cells / Q000382:microbiology*; D005260:Female; D017353:Gene Deletion; D008321:Mammary Glands, Animal / Q000166:cytology; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D037521:Virulence Factors