[Effects of anthocyanins derived from Xinjiang black mulberry fruit on delaying aging].
OBJECTIVE
To study the effects of anthocyanins from Xinjiang black mulberry fruit on the lifespan and antioxidant system of Drosophila melanogaster.


METHODS
Drosophila were divided into five groups based on the content of anthocyanins in culture medium. The lifespan of drosophila was tested by survival experiments, including median lethal time, average life-span and average maximum life-span. The activity of antioxidant enzymes of drosophila, including glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and the content of maleic diadehyde (MDA), were tested after being fed for 30 days.


RESULTS
The lifespan of drosophila were prolonged with the increase of anthocyanins. Median lethal time, average lifespan and average maximum lifespan of drosophila in the groups fed with anthocyanins at the content of 27.27 mg/kg and 112.5 mg/kg were longer than those in controls, and in a dose-response relationship. The activities of GSH-Px, SOD and CAT increased and MDA content decreased significantly with the increase of anthocyanins in a dose-response relationship. The activity of three antioxidant enzymes was the highest and MDA concentration was the lowest in the group fed with anthocyanins at the content of 11.25 mg/100g when compared with the controls.


CONCLUSIONS
The activities of three antioxidant enzymes could be enhanced and the process of lipid peroxidation could be inhibited by anthocyanins, which might be one of the reasons for prolonging the lifetime of Drosophila melanogaster.
Jiang
Yan
Y
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. jiangyan228@sohu.com
D000375:Aging / Q000187:drug effects*; D000818:Animals; D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism / Q000502:physiology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D031625:Morus / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism
D000872:Anthocyanins; D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase