Transmission of Cyprinid herpesvirus-3 (CyHV-3) from goldfish to naïve common carp by cohabitation.
Cyprinid herpesvirus-3 (CyHV-3) has spread worldwide and has had a major impact on koi and common carp production. Previous studies on the host range of the CyHV-3 found that fish species other than koi and common carp are fully resistant to natural virus exposure. Recently, CyHV-3 was detected in goldfish (Carassius auratus auratus) that were in contact with CyHV-3 infected koi. In the present study, a specific RT-PCR product was amplified from the viral thymidine kinase gene in gills, intestine and brain tissues of CyHV-3 infected goldfish. This implied that CyHV-3 replicated in these goldfish. Also, in the presence of a stress factor such as temperature fluctuation, the CyHV-3 infected goldfish transmitted the virus to cohabitated naïve SPF common carp. CyHV-3 DNA was detected in the cohabitated naïve carp tissues by PCR. The results of this study demonstrate that goldfish is a carrier for CyHV-3, permit virus propagation, and disseminate the virus to susceptible carp causing the disease.
El-Matbouli
Mansour
M
Fish Medicine and Livestock Management, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. Mansour.El-Matbouli@vetmeduni.ac.at
Soliman
Hatem
H
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005393:Fish Diseases / Q000635:transmission* / Q000821:virology; D006054:Goldfish / Q000821:virology*; D006564:Herpesviridae / Q000235:genetics / Q000472:pathogenicity*; D006566:Herpesviridae Infections / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D004279:DNA, Viral; D012367:RNA, Viral