Activity-directed identification of maize kernel peroxidases associated with postharvest insect resistance.
Maize grain peroxidases are associated with insect resistance. In this study we present an activity directed approach for the identification of peroxidases localized in different grain tissues. With drastically reduced proteomic effort we could identify different peroxidases for maize weevil susceptible and resistant genotypes of maize. Our strategy also allows for the identification of previously unknown plant peroxidases.
Winkler
Robert
R
Department of Biotechnology and Food Engineering, Tecnológico de Monterrey, ITESM-Campus Monterrey, Eugenio Garza Sada 2501, CP 64849, Monterrey, NL, Mexico.
García-Lara
Silverio
S
D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D017386:Sequence Homology, Amino Acid; D048889:Weevils / Q000502:physiology*; D003313:Zea mays / Q000201:enzymology*
D010544:Peroxidases