title: Streptococcus salivarius mutants defective in mannose phosphotransferase systems show reduced sensitivity to mutacins I-T9 and R-3B.
abstract: Twenty-four mutacin-producing Streptococcus mutans strains were screened for their propensity to produce class II one-peptide bacteriocin using a deferred antagonism assay. Streptococcus salivarius and 3 mutants defective in their mannose phosphotransferase systems (mannose-PTS) were used as sensitive strains to identify which mannose-PTS could act as the docking site for class II one-peptide bacteriocin activity. We observed that only 2 strains of S. mutans, T9 and 3B, potentially produce class II one-peptide bacteriocin, namely mutacins I-T9 and R-3B, but with no preference for any mannose-PTS complex as a target.
authors: Département de biochimie, microbiologie et bioinformatique, Faculté des sciences et de génie, Université Laval, Québec, QC G1K 7P4, Canada. guillaume.nicolas.1@ulaval.ca
authors: Guillaume G
authors: GG
authors: Nicolas
authors: Michel
authors: M
authors: Frenette
authors: Marc C
authors: MC
authors: Lavoie
mesh_terms: D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000096:biosynthesis*; D008358:Mannose / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D012463:Saliva / Q000382:microbiology; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics
chemical_list: D001430:Bacteriocins; D010455:Peptides; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D008358:Mannose