title: HIV type 1 pol gene diversity and genotypic antiretroviral drug resistance mutations in Malabo, Equatorial Guinea.
abstract: In this study, HIV strains circulating among military personnel were characterized, in Malabo, the capital city of Equatorial Guinea. One sample was found to be HIV-2 group A while a high degree of genetic diversity was recorded in the pol region of 41 HIV-1-positive samples. CRF02_AG accounted for 53.7% of the strains, and 11 different variants were obtained in the remaining 19 samples: subtype G (n = 3), A3 (n = 2), C (n = 2), CRF26_A5U (n = 2), F2 (n = 1), CRF06 (n = 1), CRF09 (n = 1), CRF11 (n = 1), CRF22 (n = 1), and divergent subtype A (n = 1) and F (n = 1). One strain could not be classified and three were unique recombinants. Analysis of antiretroviral drug resistance mutations revealed two patients each harboring one major mutation, M46I in protease and D67N in reverse transcriptase sequences, respectively. The high genetic diversity and emerging ARV resistance mutations call for frequent surveys and appropriate monitoring of ARV considering the increasing access to ARV in the country.
authors: Biotechnology Center & Department of Biochemistry, University of Yaounde I, Yaounde, Cameroon.
authors: Cyrille F
authors: CF
authors: Djoko
authors: Nathan D
authors: ND
authors: Wolfe
authors: Nicole
authors: N
authors: Vidal
authors: Ubald
authors: U
authors: Tamoufe
authors: Celine
authors: C
authors: Montavon
authors: Matthew
authors: M
authors: LeBreton
authors: Brian L
authors: BL
authors: Pike
authors: Joseph
authors: J
authors: Fair
authors: Wilfred F
authors: WF
authors: Mbacham
authors: Alogos
authors: A
authors: Benito
authors: Anne W
authors: AW
authors: Rimoin
authors: Karen
authors: K
authors: Saylors
authors: Eitel
authors: E
authors: Mpoudi-Ngole
authors: Michael P
authors: MP
authors: Grillo
authors: Martine
authors: M
authors: Peeters
mesh_terms: D000293:Adolescent; D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use; D024882:Drug Resistance, Viral / Q000235:genetics*; D016703:Equatorial Guinea; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008889:Military Personnel; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D020539:Sequence Analysis, Protein; D055815:Young Adult; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D019380:Anti-HIV Agents; D054302:pol Gene Products, Human Immunodeficiency Virus