Miniaturized video-rate epi-third-harmonic-generation fiber-microscope.
With a micro-electro-mechanical system (MEMS) mirror, we successfully developed a miniaturized epi-third-harmonic-generation (epi-THG) fiber-microscope with a video frame rate (31 Hz), which was designed for in vivo optical biopsy of human skin. With a large-mode-area (LMA) photonic crystal fiber (PCF) and a regular microscopic objective, the nonlinear distortion of the ultrafast pulses delivery could be much reduced while still achieving a 0.4 microm lateral resolution for epi-THG signals. In vivo real time virtual biopsy of the Asian skin with a video rate (31 Hz) and a sub-micron resolution was obtained. The result indicates that this miniaturized system was compact enough for the least invasive hand-held clinical use.
Chia
Shih-Hsuan
SH
Department of Electrical Engineering, Graduate Inst of Photonics and Optoelectronics, Natl Taiwan Univ, Taipei 10617, Taiwan.
Yu
Che-Hang
CH
Lin
Chih-Han
CH
Cheng
Nai-Chia
NC
Liu
Tzu-Ming
TM
Chan
Ming-Che
MC
Chen
I-Hsiu
IH
Sun
Chi-Kuang
CK
D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems; D008853:Microscopy / Q000295:instrumentation*; D008904:Miniaturization; D055100:Optical Fibers; D017785:Photons; D014743:Videotape Recording / Q000295:instrumentation*