title: Esterification activity and conformation studies of Burkholderia cepacia lipase in conventional organic solvents, ionic liquids and their co-solvent mixture media.
abstract: In this work, experiments were carried out to evaluate the esterification activity and conformation of lipase from Burkholderia cepacia in the selected conventional organic solvents, ionic liquids and their co-solvent mixture media. The results revealed that the activity of esterification of B. cepacia lipase was mostly highest in co-solvent mixture of ionic liquids-organic solvents, followed by conventional organic solvents and ionic liquids. Hence, co-solvent mixture was a high-effective strategy to enhance the activity of B. cepacia lipase for non-aqueous enzymology reaction. Conformational studies via circular dichroism spectroscopy indicated that the secondary structure of B. cepacia lipase was variant in the above-mentioned media, especially the content of alpha-helix, which was probably responsible for lipase activity difference.
authors: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors: Shaotao
authors: S
authors: Pan
authors: Xue
authors: X
authors: Liu
authors: Yadong
authors: Y
authors: Xie
authors: Yuyin
authors: Y
authors: Yi
authors: Chong
authors: C
authors: Li
authors: Yunjun
authors: Y
authors: Yan
authors: Yun
authors: Y
authors: Liu
mesh_terms: D016956:Burkholderia cepacia / Q000187:drug effects / Q000201:enzymology*; D004951:Esterification / Q000187:drug effects; D052578:Ionic Liquids / Q000494:pharmacology*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D009930:Organic Chemicals / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D012997:Solvents / Q000494:pharmacology*
chemical_list: D052578:Ionic Liquids; D009930:Organic Chemicals; D012997:Solvents; D008049:Lipase