DJ-1, an oncogene and causative gene for familial Parkinson's disease, is essential for SV40 transformation in mouse fibroblasts through up-regulation of c-Myc.
Simian virus 40 (SV40) is a tumor virus and its early gene product large T-antigen (LT) is responsible for the transforming activity of SV40. Parkinson's disease causative gene DJ-1 is also a ras-dependent oncogene, but the mechanism of its oncogene function is still not known. In this study, we found that there were no transformed foci when fibroblasts from DJ-1-knockout mice were transfected with LT. We also found that DJ-1 directly bound to LT and that the expression level of c-Myc in transformed cells was parallel to that of DJ-1. These findings indicate that DJ-1 is essential for SV40 transformation.
Kim
Yun Chul
YC
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
Kitaura
Hirotake
H
Iguchi-Ariga
Sanae M M
SM
Ariga
Hiroyoshi
H
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D005347:Fibroblasts; D051379:Mice; D018345:Mice, Knockout; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D010300:Parkinson Disease / Q000235:genetics; D054464:Peroxiredoxins; D027601:Polyomavirus Infections / Q000235:genetics / Q000378:metabolism*; D000071617:Protein Deglycase DJ-1; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D013539:Simian virus 40; D014412:Tumor Virus Infections / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D000952:Antigens, Polyomavirus Transforming; C489428:Myc protein, mouse; D015513:Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D054464:Peroxiredoxins; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1