title: [Expression of partial S gene of Hantavirus Z10 in Pichia pastoris].
abstract: OBJECTIVE
  The purpose of this study is to express partial S gene of Hantavirus Z10.
  
  
  METHODS
  The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.
  
  
  RESULTS
  The recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.
  
  
  CONCLUSIONS
  The nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.
authors: The Key Lab of HFRS of Zhejiang Province, The Center for Disease Prevention and Control of Zhejiang Province, Hangzhou, China. hanpingzhu@yahoo.com.cn
authors: Han-ping
authors: HP
authors: Zhu
authors: Ping-ping
authors: PP
authors: Yao
authors: Xiao-zhao
authors: XZ
authors: Deng
authors: Fang
authors: F
authors: Xu
authors: Rong-hui
authors: RH
authors: Xie
authors: Zhi-yong
authors: ZY
authors: Zhu
authors: Yun
authors: Y
authors: Zhang
mesh_terms: D015870:Gene Expression; D006476:Orthohantavirus / Q000235:genetics* / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism
chemical_list: D019590:Nucleocapsid Proteins