title: Organization of Pseudomonas fluorescens on chemically different nano/microstructured surfaces.
abstract: This paper describes bacterial organization on nano/micropatterned surfaces with different chemical properties, which show different interactions with the biological systems (inert, biocompatible, and bactericide). These surfaces were prepared by molding techniques and exposed to Pseudomonas fluorescens (P. fluorescens) cultures. Results from atomic force microscopy and optical imaging demonstrate that the structure of P. fluorescens aggregates is strongly dependent on the surface topography while there is no clear linking with the physical-chemical surface properties (charge and contact angle) of the substrate immersed in abiotic culture media. We observe that regardless of the material when the surface pattern matches the bacterial size, bacterial assemblages involved in surface colonization are disorganized. The fact there is not a relationship between surface chemistry and bacterial organization can be explained by the coverage of the surfaces by adsorbed organic species coming from the culture medium. Viability assays indicate that copper behaves as a toxic substrate despite the presence of adsorbed molecules. The combination of surface traps and biocidal activity could act synergistically as a suitable strategy to limit bacterial spreading on implant materials.
authors: Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Casilla de Correo 16, Sucursal 4, (1900) La Plata, Argentina.
authors: Carolina
authors: C
authors: Díaz
authors: Roberto C
authors: RC
authors: Salvarezza
authors: Mónica A
authors: MA
authors: Fernández Lorenzo de Mele
authors: Patricia L
authors: PL
authors: Schilardi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002449:Cell Aggregation / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D016764:Cell Polarity; D008904:Miniaturization; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011551:Pseudomonas fluorescens / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials