title: Effects of lindane on the accumulation of cyclic AMP, induced by vasoactive intestinal peptide, in isolated rat prostatic epithelial cells.
abstract: Lindane pretreatment of isolated rat prostatic epithelial cells resulted in a time- and dose-dependent impairment of the stimulation of cyclic AMP levels by vasoactive intestinal peptide (VIP); the optimal conditions for producing this impairment were found to be 5 min of cell exposure to 0.2 mm-lindane at 25 degrees C. The inhibitory effect of the insecticide was related to a decrease in VIP efficiency since the maximal cyclic AMP response (at 100 nm VIP) was about 50% of that in control cells. VIP potency was unaffected since the half-maximal cyclic AMP response was elicited at about 3 nm VIP in both the control and lindane-pretreated cells. The results indicate that lindane interferes with the cyclic AMP system in prostatic epithelium, and this could be a consequence of some interaction in the prostate gland between the two main systems for signal transduction (i.e. adenylate cyclase and phosphatidylinositol/Ca(2+)/protein kinase C).
authors: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
authors: I
authors: I
authors: Carrero
authors: M A
authors: MA
authors: Pérez-Albarsanz
authors: M N
authors: MN
authors: Recio
authors: J C
authors: JC
authors: Prieto