γ-H2AX and phosphorylated ATM focus formation in cancer cells after laser plasma X irradiation.
The usefulness of laser plasma X-ray pulses for medical and radiation biological studies was investigated, and the effects of laser plasma X rays were compared with those of conventional sources such as a linear accelerator. A cell irradiation system was developed that used copper-Kα (8 keV) lines from an ultrashort high-intensity laser to produce plasma. The absorbed dose of the 8 keV laser plasma X-ray pulse was estimated accurately with Gafchromic® EBT film. When the cells were irradiated with approximately 2 Gy of laser plasma X rays, the circular regions on γ-H2AX-positive cells could be clearly identified. Moreover, the numbers of γ-H2AX and phosphorylated ataxia telangiectasia mutated (ATM) foci induced by 8 keV laser plasma X rays were comparable to those induced by 4 MV X rays. These results indicate that the laser plasma X ray source may be useful for radiation biology studies.
Sato
Katsutoshi
K
Department of Medical Physics and Engineering, Graduated School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Nishikino
Masaharu
M
Okano
Yasuaki
Y
Ohshima
Shinsuke
S
Hasegawa
Noboru
N
Ishino
Masahiko
M
Kawachi
Tetsuya
T
Numasaki
Hodaka
H
Teshima
Teruki
T
Nishimura
Hiroaki
H
D064007:Ataxia Telangiectasia Mutated Proteins; D001940:Breast / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D008853:Microscopy; D009154:Mutation / Q000528:radiation effects*; D010766:Phosphorylation / Q000528:radiation effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011829:Radiation Dosage; D025521:Tumor Suppressor Proteins / Q000378:metabolism*; D014965:X-Rays
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C468783:H2AX protein, human; D006657:Histones; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases