Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca(2+)-ATPase and ryanodine receptor-2 mRNA expression in heart transplant recipients.
The aim of the present study was to investigate the relationship between rejections and gene expression of Ca(2+)-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR(+) (n = 9) with rejection grade of 2 or higher versus group AR(-) (n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR(+) (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR(-) (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR(+) and group AR(-); however, they were reduced in group Rec-AR(+) more than in group Rec-AR(-) (0.83 +/- 0.07 versus 0.90 +/- 0.07, P = 0.034, 0.74 +/- 0.06 versus 0.84 +/- 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca(2+)-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca(2+)-handling proteins in heart transplant recipients.
Kato
Tomoko S
TS
Department of Cardiovascular Medicine, National Cardiovascular Center, Suita, Osaka, Japan.
Komamura
Kazuo
K
Sata
Yusuke
Y
Ishibashi-Ueda
Hatsue
H
Ikeda
Yoshihiko
Y
Matsuyama
Taka-aki
TA
Izawa
Hideo
H
Yokota
Mitsuhiro
M
Shishido
Toshiaki
T
Nakatani
Takeshi
T
Kitamura
Soichiro
S
Asakura
Masaki
M
Hashimura
Kazuhiko
K
Kitakaze
Masafumi
M
D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D016027:Heart Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases