Strategies of poly(3-hydroxybutyrate) synthesis by Haloarcula sp. IRU1 utilizing glucose as carbon source: Optimization of culture conditions by Taguchi methodology.
We investigated optimization of poly(3-hydroxybutyrate) (PHB) production by Haloarcula sp. IRU1 in a batch culture system. Various batch experiments were carried out at different carbon source concentrations [glucose, at 1-8% (w/v)], phosphorus source concentrations [K(2)HPO(4), KH(2)PO(4), Na(3)PO(4) and Na(2)HPO(4) at 0.001-0.016% (w/v)], nitrogen source concentrations [NH(4)Cl, yeast extract, peptone and tryptone at 0.1-0.8% (w/v)] and temperatures [37-55°C]. By these experiments, optimum production conditions were determined using the Taguchi method. The results showed the highest PHB production by Haloarcula sp. IRU1 (63.0% of cell dry weight) in the presence of 2g/l glucose, 0.2g/l NH(4)Cl, 0.004 g/l KH(2)PO(4) and temperature (42°C). In overall, the results of this study showed that Haloarcula sp. IRU1 has a high potential for synthesis of PHB from glucose.
Taran
Mojtaba
M
Laboratory of Microbiology, Department of Biology, Faculty of Science, Razi University, P.O. Box 6714967346, Kermanshah, Iran. mtaran@razi.ac.ir
Amirkhani
Homeira
H
D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism*; D019613:Haloarcula / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000138:chemical synthesis*; D011091:Polyesters / Q000138:chemical synthesis*; D000094122:Polyhydroxybutyrates
D002244:Carbon; D003470:Culture Media; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates