Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA.
BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.
Liu
Jie
J
Department of Microbiology, University of California-Davis, Davis, CA, USA.
Doty
Tammy
T
Gibson
Bryan
B
Heyer
Wolf-Dietrich
WD
D051017:Apoptosis Regulatory Proteins; D024682:BRCA2 Protein / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D002846:Chromatography, Affinity; D004260:DNA Repair / Q000502:physiology; D004270:DNA, Circular / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D046988:Proteasome Endopeptidase Complex / Q000502:physiology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051135:Rad51 Recombinase / Q000737:chemistry / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology; D011995:Recombination, Genetic / Q000502:physiology; D051716:Replication Protein A
D051017:Apoptosis Regulatory Proteins; C118024:BLID protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D011993:Recombinant Fusion Proteins; D051716:Replication Protein A; C484606:SEM1 protein, human; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D046988:Proteasome Endopeptidase Complex