Heparanase expression in term placentas of diabetic patients and healthy controls.
OBJECTIVE
The prevalence of diabetic disorders in pregnancy is rising, which goes along with increased risks for maternal and foetal complications during pregnancy and delivery. The expression of the endo-β-D: -glucuronidase, heparanase (HPSE), may increase under hyperglycaemic conditions, is believed to play an important role in diabetes associated morbidity outside the female reproductive tract and is expressed in the placenta throughout gestation. However, the placental expression of HPSE has not been investigated in diabetic patients.


METHODS
Placental biopsies of 30 patients with pre-existing or gestational diabetes and 30 healthy controls were analysed by real-time PCR and immunohistochemistry with regard to the presence of HPSE at term.


RESULTS
Patients and controls were comparable with respect to foetal outcome and maternal characteristics except for maternal body mass index. We were unable to show significant differences in placental HPSE expression between diabetic patients and healthy controls.


CONCLUSIONS
This study suggests that HPSE expression in term placentas is not affected by maternal diabetes and thus does not contribute to pathological processes in diabetic pregnancies with deliveries at term.
Luedders
Doerte W
DW
Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, Campus Luebeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Bundschuh
Diana
D
Hornemann
Amadeus
A
Hoerster
Jana-Christin
JC
Boemicke
Philipp
P
Köster
Frank
F
Bohlmann
Michael K
MK
D000328:Adult; D001034:Apgar Score; D001724:Birth Weight; D015992:Body Mass Index; D002585:Cesarean Section; D016640:Diabetes, Gestational / Q000201:enzymology*; D005260:Female; D005865:Gestational Age; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008875:Middle Aged; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D018709:Statistics, Nonparametric; D055815:Young Adult; D000099175:Heparanase
D005966:Glucuronidase; D000099175:Heparanase