Combined technologies for microfabricating elastomeric cardiac tissue engineering scaffolds.
Polymer scaffolds that direct elongation and orientation of cultured cells can enable tissue engineered muscle to act as a mechanically functional unit. We combined micromolding and microablation technologies to create muscle tissue engineering scaffolds from the biodegradable elastomer poly(glycerol sebacate). These scaffolds exhibited well defined surface patterns and pores and robust elastomeric tensile mechanical properties. Cultured C2C12 muscle cells penetrated the pores to form spatially controlled engineered tissues. Scanning electron and confocal microscopy revealed muscle cell orientation in a preferential direction, parallel to micromolded gratings and long axes of microablated anisotropic pores, with significant individual and interactive effects of gratings and pore design.
Guillemette
Maxime D
MD
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Park
Hyoungshin
H
Hsiao
James C
JC
Jain
Saloni R
SR
Larson
Benjamin L
BL
Langer
Robert
R
Freed
Lisa E
LE
D000818:Animals; D002460:Cell Line; D048430:Cell Shape; D003651:Decanoates / Q000138:chemical synthesis / Q000737:chemistry; D055119:Elastic Modulus; D020724:Elastomers / Q000138:chemical synthesis* / Q000737:chemistry; D005990:Glycerol / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D008567:Membranes, Artificial; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D055616:Microtechnology / Q000379:methods*; D032446:Myoblasts / Q000166:cytology; D009206:Myocardium / Q000166:cytology*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D003651:Decanoates; D020724:Elastomers; D008567:Membranes, Artificial; D011108:Polymers; C469892:poly(glycerol-sebacate); D005990:Glycerol