Enzyme-linked immunosorbent serum assays (ELISAs) for rat and human N-terminal pro-peptide of collagen type I (PINP)--assessment of corresponding epitopes.
OBJECTIVE
The present study describes two newly developed N-terminal pro-peptides of collagen type I (PINP) competitive enzyme-linked immunosorbent assays (ELISAs) for the assessment of corresponding PINP epitopes in the rat- and human species.


METHODS
Monoclonal antibodies were raised against corresponding rat and human PINP sequences and competitive assays were developed for each species. They were evaluated in relevant pre-clinical or clinical studies.


RESULTS
The antibody characterizations indicated that PINP indeed was recognized. Technical robust assays were obtained. Rat PINP and tALP showed similar patterns in the gold standard osteoporosis rat ovariectomized (OVX) model. No liver contribution was observed in the liver fibrosis rat bile duct ligation model (BDL). In an osteoporosis study, the human serum PINP levels were significantly decreased after ibandronate treatment compared to placebo.


CONCLUSIONS
The two corresponding PINP assays were specific and these bone turnover markers may improve translational science for the evaluation for bone-related diseases.
Leeming
D J
DJ
Nordic Bioscience A/S, Herlev, Denmark; Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark. djl@nordicbioscience.com
Larsen
D V
DV
Zhang
C
C
Hi
Y
Y
Veidal
S S
SS
Nielsen
R H
RH
Henriksen
K
K
Zheng
Q
Q
Barkholt
V
V
Riis
B J
BJ
Byrjalsen
I
I
Qvist
P
P
Karsdal
M A
MA
D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002138:Calibration; D002999:Clone Cells; D003710:Demography; D004164:Diphosphonates / Q000008:administration & dosage / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D000077557:Ibandronic Acid; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D010919:Placebos; D017698:Postmenopause / Q000097:blood / Q000187:drug effects; D011347:Procollagen / Q000097:blood* / Q000737:chemistry / Q000276:immunology*; D051381:Rats
D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D000939:Epitopes; D010446:Peptide Fragments; D010919:Placebos; D011347:Procollagen; C047202:procollagen Type I N-terminal peptide; D015675:Osteocalcin; D000077557:Ibandronic Acid