MicroRNA-10b induced by Epstein-Barr virus-encoded latent membrane protein-1 promotes the metastasis of human nasopharyngeal carcinoma cells.
MicroRNA-10b (miR-10b) has been reported to facilitate the metastasis of breast cancer. However, little is known about the role of miR-10b in the metastasis of nasopharyngeal carcinoma (NPC). Here, we show that high levels of miR-10b expression in Epstein-Barr virus (EBV)-positive latent membrane protein-1 (LMP1)-expressing NPC cells, and its expression is down-regulated by silencing LMP1 or Twist. Induction of miR-10b over-expression in LMP1-silent C666-1 cells promoted significant wound healing and transmembrane invasiveness in vitro. More importantly, miR-10b over-expression promoted the metastasis of NPC and accelerated the death of tumor-bearing nude mice. These findings strongly suggest that miR-10b positively regulates the metastasis of NPC.
Li
Gang
G
Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Wu
Zhengrong
Z
Peng
Ying
Y
Liu
Xiong
X
Lu
Juan
J
Wang
Lu
L
Pan
Qiuhui
Q
He
Min-Liang
ML
Li
Xiang-Ping
XP
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000473:pathology* / Q000821:virology; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D051879:Twist-Related Protein 1 / Q000502:physiology; D014763:Viral Matrix Proteins / Q000032:analysis / Q000502:physiology*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D051879:Twist-Related Protein 1; D014763:Viral Matrix Proteins