The Dad1 subunit of the yeast kinetochore Dam1 complex is an intrinsically disordered protein.
The Dam1 complex is an important part of the yeast kinetochore. It mediates attachment of the chromosome to the mitotic spindle and is involved in chromatid separation initiated at anaphase. It is comprised of 10 individual subunits and has been observed to oligomerize in various ways as it interacts with microtubules, including forming a ring. This work explores the biochemical and biophysical properties of Dad1, one of the Dam1 complex subunits from the human fungal pathogen Candida albicans. Unlike its Saccharomyces cerevisiae counterpart, C. albicans Dad1 can be expressed as a soluble protein in Escherichia coli. Analysis of this protein's hydrodynamic properties, thermostability and primary sequence have been conducted. As a result, we conclude that isolated Dad1 is an intrinsically disordered protein.
Waldo
Jennifer Turner
JT
State University of New York at New Paltz, Biology Department, 1 Hawk Drive, New Paltz, NY 12561, USA. waldoj@newpaltz.edu
Greagor
Stacy A
SA
Iqbal
Aruba J
AJ
Gittens
Abiola S A
AS
Grant
Kemone K
KK
D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D055550:Protein Stability; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C439311:Dad1 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins