Methylation patterns of Brahma during spermatogenesis and oogenesis: potential implications.
To compare methylation profiles and expression levels of Brahma at different stages of spermatogenesis, and to identify the methylation pattern during oogenesis, we analyzed gene expression and methylation patterns in murine germ cells at various developmental stages. The methylation levels of CpG islands within Brahma increased during spermatogenesis and decreased during oogenesis. This change in methylation pattern correlates with the change in expression of Brahma during spermatogenesis. As the degree of methylation increases, the expression decreases. The change in methylation is opposite during oogenesis, which suggests opposite expression levels.
Nagrani
Sohan R
SR
Laboratory of Clinical and Developmental Genomics, Program in Reproductive and Adult Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20814, USA. nagranisr@mail.nih.gov
Levens
Eric D
ED
Baxendale
Vanessa
V
Boucheron
Catherine
C
Chan
Wai Yee
WY
Rennert
Owen M
OM
D002843:Chromatin / Q000502:physiology; D018899:CpG Islands / Q000502:physiology; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D006801:Humans; D007985:Leydig Cells / Q000502:physiology; D008297:Male; D009865:Oocytes / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D012708:Sertoli Cells / Q000502:physiology; D013090:Spermatocytes / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*
D002843:Chromatin; C084011:SMARCA2 protein, human; D014157:Transcription Factors