title: Background bisphenol A in experimental materials and its implication to low-dose in vitro study.
abstract: In vitro low-dose studies are important to understand the mechanisms of bisphenol A (BPA) action. BPA doses used in current in vitro studies varied considerably, and doses as low as 10(-15)M have been reported. The actual doses of BPA used in the in vitro low-dose studies were rarely checked analytically, and the background BPA levels in experimental materials, which will determine the lowest BPA dose to be used, should be investigated or considered. In this study, the background BPA levels in various materials typically used in in vitro low-dose studies for BPA were investigated. Background BPA levels from the use of disposable pipettes and pipette tips were low (<0.20 ng mL(-1) or 0.88 nM). BPA was also detected in several commercial buffer solutions at levels close to the method limit of quantification (LOQ) (0.02 ng mL(-1); 0.088 nM). However, BPA was detected in all cell culture media obtained from various sources at levels ranging from 0.080 to 4.26 ng mL(-1) (or 0.35 to 19 nM) with an average of 0.83 ng mL(-1) (3.5 nM). We suggest that culture media used for low-dose BPA studies should be analysed for background BPA levels prior to use, and the medium with the lowest BPA levels should be used.
authors: Food Research Division, Bureau of Chemical Safety, Food Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, Ontario, Canada. Xu-Liang.Cao@hc-sc.gc.ca
authors: Xu-Liang
authors: XL
authors: Cao
authors: Jeannette
authors: J
authors: Corriveau
authors: Svetlana
authors: S
authors: Popovic
authors: Melanie C
authors: MC
authors: Coughlan
authors: Nikolai
authors: N
authors: Chepelev
authors: William
authors: W
authors: Willmore
authors: Timothy
authors: T
authors: Schrader
authors: Xiaolei
authors: X
authors: Jin
mesh_terms: D001559:Benzhydryl Compounds; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D011126:Polypropylenes / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D018675:Toxicity Tests; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; D011126:Polypropylenes; D011137:Polystyrenes; C006780:bisphenol A; D000098826:Bisphenol A Compounds