Selective inhibition of mammalian diamine oxidase by aquatic biotoxins.
The inhibitory activity of saxitoxin and tetrodotoxin on diamine oxidase and S-adenosyl-l-methionine decarboxylase from mammalian sources have been analysed. Unlike tetrodotoxin, saxitoxin was a reversible non-competitive inhibitor of pig kidney diamine oxidase with an estimated K(i) of 140 mum. S-Adenosyl-l-methionine decarboxylase from a highly purified source was not affected by the concentrations tested of either biotoxin. A possible analytical application of this finding is discussed.
Cubria
J C
JC
Departamento de Fisiologia, Farmacologia y Toxicologia, Facultad de Veterinaria, Universidad de León, Campus de Vegazana, 24071 León, Spain.
Fouce
R B
RB
Ortiz
A
A
Alvarez-Bujidos
M L
ML
Ordoñez
D
D