A new method for the detection of the H5 influenza virus by magnetic beads capturing quantum dot fluorescent signals.
A new method to detect H5 influenza virus using quantum dots (QDs) and magnetic beads (MBs) is described. QDs conjugated with oligonucleotide probes were used to produce fluorescent signals and MBs, that were also conjugated with probes, were used to isolate and concentrate the signals. Target viral RNAs led to a sandwich hybridization between the functionalized QDs and MBs. One-step hybridization facilitated the subtype determination. As little as 0.1 ng viral RNA could be detected.
Zhang
Wentao
W
State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, 430072, Wuhan, People's Republic of China.
Wu
Di
D
Wei
Jianghua
J
Xiao
Gengfu
G
D005453:Fluorescence; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis* / Q000737:chemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics; D045663:Quantum Dots; D012367:RNA, Viral / Q000235:genetics; D014773:Virology / Q000379:methods*
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D015345:Oligonucleotide Probes; D012367:RNA, Viral; C109963:hemagglutinin, avian influenza A virus