title: Caspase-3 and Bcl-2 expression in glioblastoma: an immunohistochemical study.
abstract: The unfavorable prognosis of malignant gliomas can also be explained by the incomplete knowledge of their molecular pathways. Studies regarding the regulatory process of apoptosis in glioblastoma (GBM), the most common malignant glioma, are few, and better knowledge of the expression of pro and anti-apoptotic proteins could collaborate with the development of new treatments founded on molecular basis. The objective of this study was to evaluate by immunohistochemistry the expression of caspase-3 and Bcl-2 in 30 samples of GBMs. The expression of caspase-3 (mean 17.67%) was lower than Bcl-2 (mean 30.92%), a statistically significant result (p<0.0001), suggesting low apoptotic activity in these tumors. Other studies of proteins related to the intrinsic and extrinsic pathway of apoptosis are required to provide additional information of this mechanism in GBMs.
authors: Department of Surgery and Anatomy, Medicine School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. tirapeli@fmrp.usp.br
authors: Luís Fernando
authors: LF
authors: Tirapelli
authors: Paulo Henrique Nasser Andre
authors: PH
authors: Bolini
authors: Daniela Pretti da Cunha
authors: DP
authors: Tirapelli
authors: Fernanda Maris
authors: FM
authors: Peria
authors: Aline Nazareth Paixão
authors: AN
authors: Becker
authors: Fabiano Pinto
authors: FP
authors: Saggioro
authors: Carlos Gilberto
authors: CG
authors: Carlotti
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D005260:Female; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3