Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.
There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl(2) administration. Decreasing the concentration of Ca(++) in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca(++) concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.
Ishikawa
Mayumi
M
Division of Diabetes, Metabolism and Endocrinology, Toho University School of Medicine, 6-11-1, Omori-Nishi, Ota-Ku, Tokyo 143-8541, Japan. m-ishikawa@med.toho-u.ac.jp
Kimura
Kazuhiro
K
Tachibana
Toshiaki
T
Hashimoto
Hisashi
H
Shimojo
Masako
M
Ueshiba
Hajime
H
Tsuboi
Kumiko
K
Shibuya
Kazutoshi
K
Yoshino
Gen
G
D000328:Adult; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology; D007621:Karyotyping; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008858:Microscopy, Phase-Contrast; D010281:Parathyroid Hormone / Q000378:metabolism*; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000378:metabolism*; D044169:Receptors, Calcium-Sensing / Q000032:analysis; D055752:Small Cell Lung Carcinoma / Q000378:metabolism* / Q000473:pathology
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D044169:Receptors, Calcium-Sensing; D011333:Pro-Opiomelanocortin