Conditional deletion of histone deacetylase 1 in T cells leads to enhanced airway inflammation and increased Th2 cytokine production.
Chromatin modifications, such as reversible histone acetylation, play a key role in the regulation of T cell development and function. However, the role of individual histone deacetylases (HDACs) in T cells is less well understood. In this article, we show by conditional gene targeting that T cell-specific loss of HDAC1 led to an increased inflammatory response in an in vivo allergic airway inflammation model. Mice with HDAC1-deficient T cells displayed an increase in all critical parameters in this Th2-type asthma model, such as eosinophil recruitment into the lung, mucus hypersecretion, parenchymal lung inflammation, and enhanced airway resistance. This correlated with enhanced Th2 cytokine production in HDAC1-deficient T cells isolated from diseased mice. In vitro-polarized HDAC1-deficient Th2 cells showed a similar enhancement of IL-4 expression, which was evident already at day 3 of Th2 differentiation cultures and restricted to T cell subsets that underwent several rounds of cell divisions. HDAC1 was recruited to the Il4 gene locus in ex vivo isolated nonstimulated CD4(+) T cells, indicating a direct control of the Il4 gene locus. Our data provide genetic evidence that HDAC1 is an essential HDAC that controls the magnitude of an inflammatory response by modulating cytokine expression in effector T cells.
Grausenburger
Reinhard
R
Department of Medical Biochecmistry, Max F. Perutz Laboratories, Vienna Biocenter, Vienna, Austria.
Bilic
Ivan
I
Boucheron
Nicole
N
Zupkovitz
Gordin
G
El-Housseiny
Lamia
L
Tschismarov
Roland
R
Zhang
Yu
Y
Rembold
Martina
M
Gaisberger
Martin
M
Hartl
Arnulf
A
Epstein
Michelle M
MM
Matthias
Patrick
P
Seiser
Christian
C
Ellmeier
Wilfried
W
D000818:Animals; D016764:Cell Polarity / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004195:Disease Models, Animal; D056284:Histone Deacetylase 1 / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D007249:Inflammation / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000201:enzymology / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012130:Respiratory Hypersensitivity / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D018418:Th2 Cells / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*
D016207:Cytokines; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1