Surface-enhanced Raman spectroscopy as a tool for detecting Ca2+ mobilizing second messengers in cell extracts.
Understanding of calcium signaling pathways in cells is essential for elucidating the mechanisms of both normal cell function and cancer development. Calcium messengers play the crucial role for intracellular Ca(2+) release. We propose a new approach to detecting the calcium second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) in cell extracts using surface-enhanced Raman spectroscopy (SERS). Currently available radioreceptor binding and enzymatic assays require extensive sample preparation and take more than 12 h. With a SERS sensor, NAADP can be detected in less than 1 min without any special sample preparation. To the best of our knowledge, this is the first demonstration of using SERS for calcium signaling applications.
Vitol
Elina A
EA
Department of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA.
Brailoiu
Eugen
E
Orynbayeva
Zulfiya
Z
Dun
Nae J
NJ
Friedman
Gary
G
Gogotsi
Yury
Y
D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D045744:Cell Line, Tumor; D036563:Cyclic ADP-Ribose / Q000737:chemistry / Q000502:physiology; D006046:Gold / Q000737:chemistry; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000737:chemistry / Q000502:physiology; D053768:Metal Nanoparticles / Q000737:chemistry; D009249:NADP / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000502:physiology; D015290:Second Messenger Systems; D013059:Spectrum Analysis, Raman / Q000379:methods*
D036563:Cyclic ADP-Ribose; D009249:NADP; C024376:NAADP; D006046:Gold; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium