[Clonal relationship of Hodgkin lymphoma and its recurrence].
In this study, we investigated whether recurrences of classical Hodgkin's lymphoma (HL) are true relapses arising from the primary tumour or clonally unrelated secondary neoplasias. Formalin-fixed, paraffin-embedded tissue specimens of eleven patients with recurrent HL were analyzed. Hodgkin and Reed-Sternberg cells were microdissected after immunohistochemical staining for CD30 using laser-capture technique. Immunoglobulin heavy chain (IgH) gene fragment lengths were analyzed applying consensus FR3 and J primers. Two early relapses after the first HL diagnosis were clonally related to the initial tumour, while three of four early recurrences after a first or second relapse were not. Three patients presenting with late relapses had clonally unrelated neoplasms. Therefore, we conclude that recurrent HL may represent a novel neoplasm, a finding which might play a role in clinical decision-making.
Obermann
E C
EC
Institut für Pathologie, Universitätsspital Basel, Schönbeinstr. 40, 4031 Basel, Schweiz. EObermann@uhbs.ch
Müller
N
N
Rufle
A
A
Menter
T
T
Dirnhofer
S
S
Tzankov
A
A
D002999:Clone Cells / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D006689:Hodgkin Disease / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology*; D016539:Reed-Sternberg Cells / Q000473:pathology
D007143:Immunoglobulin Heavy Chains