The 1H, 13C and 15N backbone and side-chain assignment of the RRM domain of SC35, a regulator of pre-mRNA splicing.
The serine-arginine rich family of proteins play important roles in the regulation of both constitutive and alternative splicing. SC35 (also known as SFRS2 and PR264) is a member of this family and contains one RNA recognition motif (RRM domain) and a RS domain at the C-terminus which is enriched with arginine and serine residues. SC35 is specifically involved in major regulatory pathways for cell proliferation and cell cycle progression. Determining the structure of SC35 would enable greater understanding of how its structure relates to its many functions. Complete (1)H, (13)C and (15)N assignments of the RRM domain of SC35 are presented. The assignments were obtained using 2D heteronuclear and 3D triple-resonance experiments with the uniformly [(15)N,(13)C]-labelled protein. The chemical shifts are used to predict the 3-dimensional structure of this RRM domain in the absence of RNA.
Clayton
Jonathan C
JC
NMR Centre for Structural Biology, School of Biological Sciences, University of Liverpool, UK.
Phelan
Marie
M
Goult
Benjamin T
BT
Hautbergue
Guillaume M
GM
Wilson
Stuart A
SA
Lian
Lu-Yun
LY
D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D006801:Humans; D006859:Hydrogen; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism*; D000068103:Serine-Arginine Splicing Factors
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009687:Nuclear Proteins; D012322:RNA Precursors; D012261:Ribonucleoproteins; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D006859:Hydrogen