title: Antigenic activity of bacterial endodontic contents from primary root canal infection with periapical lesions against macrophage in the release of interleukin-1beta and tumor necrosis factor alpha.
abstract: BACKGROUND
  Periradicular tissue chronic stimulation by endotoxin may cause apical periodontitis. This study investigated the microbial profile and the levels of endotoxin found in primary root canal infection with apical periodontitis, determined their antigenicity against macrophages through the levels of interleukin (IL)-1beta and tumor necrosis factor alpha (TNF-alpha), and evaluated their relationship with clinical and radiographic findings.
  
  
  METHODS
  Samples were taken from 21 root canals with primary endodontic infection and apical periodontitis with paper points. PCR technique (16S rDNA) was used for the detection of the target bacteria. Limulus Amebocyte Lysate (LAL) was used to measure endotoxin. The amounts of IL-1ss/ TNF-alpha in macrophages supernatants were measured by enzyme-linked immunosorbent assay--Duoset-kit (ELISA).
  
  
  RESULTS
  Prevotella nigrescens (13/21), Porphyromonas endodontalis (6/21), and Treponema socranskii (6/21) were the most frequently detected gram-negative bacterial species. The presence of the sinus tract (2/21) was related to the detection of Filifactor alocis (p < 0.05), whereas a tooth with a radiolucent area > or = 2 mm was related to the detection of Treponema denticola. A correlation was found between the number of gram-negative bacteria and the levels of IL-1beta/TNF-alpha (p < 0.05). Increased levels of endotoxin were followed by TNF-alpha release (p < 0.05). Higher levels of IL-1beta (p < 0.05) and endotoxin contents were related to the larger size of the radiolucent area.
  
  
  CONCLUSIONS
  The antigenicity of the endodontic contents is not only related to the amount of endotoxin found in the root canal but also to the number of different species of gram-negative bacteria involved in the infection. Moreover, a larger size (> or = 2 mm) of the radiolucent area was related to IL-1beta and endotoxin.
authors: Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Campinas, Brazil.
authors: Frederico C
authors: FC
authors: Martinho
authors: Wanderson Miguel Maia
authors: WM
authors: Chiesa
authors: Fabio R M
authors: FR
authors: Leite
authors: Joni A
authors: JA
authors: Cirelli
authors: Brenda P F A
authors: BP
authors: Gomes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000276:immunology* / Q000382:microbiology; D004731:Endotoxins / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000276:immunology* / Q000382:microbiology; D012333:RNA, Messenger / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004731:Endotoxins; D053583:Interleukin-1beta; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; D014409:Tumor Necrosis Factor-alpha