title: Nanofibers in a hyaluronan-based pericellular matrix.
abstract: The extracellular matrix of the brain is a highly organized hyaluronan-based supramolecular assembly that is involved in neuronal pathfinding, cell migration, synaptogenesis and neuronal plasticity. Here, we analyze the structure of the hyaluronan-rich pericellular matrix of an oligodendroglial precursor cell line using helium ion beam scanning microscopy at a subnanometer resolution. We find that thin nanofibers are the ultimate building elements of this oligodendroglial pericellular matrix. These structures may participate in the regulation of oligodendroglial maturation and motility.
authors: Institut für Biochemie und Molekularbiologie, Universität Bonn, Nussallee 11, 53115 Bonn, Germany.
authors: Debora
authors: D
authors: Gerlach
authors: Tim
authors: T
authors: Kaminski
authors: Fabián
authors: F
authors: Pérez-Willard
authors: Gregor
authors: G
authors: Kirfel
authors: Volkmar
authors: V
authors: Gieselmann
authors: Joachim
authors: J
authors: Kappler
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006820:Hyaluronic Acid / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics; D058580:Neurocan; D009836:Oligodendroglia / Q000648:ultrastructure*; D011509:Proteoglycans / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000648:ultrastructure
chemical_list: C507576:Ncan protein, mouse; D009419:Nerve Tissue Proteins; D058580:Neurocan; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006820:Hyaluronic Acid