Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains.
Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.
Pupek
Małgorzata
M
Department of Chemistry and Immunochemistry, Medical University of Wrocław, Poland.
Lemańska-Perek
Anna
A
Jasonek
Jolanta
J
Kątnik-Prastowska
Iwona
I
D000293:Adolescent; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D005353:Fibronectins / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D017510:Protein Folding; D017434:Protein Structure, Tertiary
D000939:Epitopes; D005353:Fibronectins