[Molecular genetics typing of Brucella circulating in several provinces of Mongolia].
OBJECTIVE
Comparative molecular-genetic typing of Brucella strains isolated in Mongolia from different animal species as well as from humans.


METHODS
Twenty-one strains of Brucella isolated from different hosts in 7 provinces of Mongolia were typed. Conventional phenotypic methods, genotyping by PCR with primers for genus- and species-specific differentiating targets of Brucella genes as well as multiple locus variable number tandem repeats analysis (MLVA) with 12 pairs of primers bounding locus variable tandem repeats of different length (from 134 bp to 8 bp).


RESULTS
Phenotypic identification and genotyping by PCR using primers for differentiating DNA markers allowed to attribute 14 isolates to B. melitensis biovar 2, and 7 - to B. abortus biovar 3. By using the MLVA method, connection of MLVA genotypes of 9 Brucella isolates with their reservoir hosts (sheep, cows) was shown providing their circulation in Khentii, Bulqan, and Khubsgul provinces bordering with Russia. Nine isolates from different hosts (camel, yaks, goats, sheep) isolated in Ovorkhangai, Dundgovi, and Dornogovi provinces, which have not border with Russia, had closely related MLVA genotypes indicating an opportunity of migration of pathogenic Brucella species to not-typical hosts.


CONCLUSIONS
Molecular-genetic typing of Brucella isolated in Mongolia was done for the first time; levels of their genetic relation and diversity were demonstrated. Circulation of Brucella isolated with specific MLVA genotypes was connected to territories of specific Mongolian provinces. The study proved migration of Brucella to not-typical hosts. Comparative study of isolates circulating in frontier with Mongolia areas of Russia (Irkutsk region, Tyva and Buryat Republics) are necessary to perform.
Kulakov
Iu K
IuK
Erdenebaator
J
J
Tsirelson
L E
LE
Tolmacheva
T A
TA
Zheludkov
M M
MM
Korenberg
E I
EI
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D002002:Brucella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004197:Disease Reservoirs / Q000382:microbiology* / Q000662:veterinary; D014644:Genetic Variation; D006801:Humans; D017720:Molecular Epidemiology; D008986:Mongolia / Q000453:epidemiology; D012418:Ruminants / Q000382:microbiology*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial