Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy.
OBJECTIVE
To investigate whether irradiation (IR) and partial hepatectomy (PH) may prepare the host liver for non-parenchymal cell (NPC) transplantation.


METHODS
Livers of dipeptidyl peptidase IV (DPPIV)-deficient rats were pre-conditioned with external beam IR (25 Gy) delivered to two-thirds of the right liver lobules followed by a one-third PH of the untreated lobule. DPPIV-positive liver cells (NPC preparations enriched for liver sinusoidal endothelial cells (LSECs) and hepatocytes) were transplanted via the spleen into the recipient livers. The extent and quality of donor cell engraftment and growth was studied over a long-term interval of 16 wk after transplantation.


RESULTS
Host liver staining demonstrated 3 different repopulation types. Well defined clusters of donor-derived hepatocytes with canalicular expression of DPPIV were detectable either adjacent to or in between large areas of donor cells (covering up to 90% of the section plane) co-expressing the endothelial marker platelet endothelial cell adhesion molecule. The third type consisted of formations of DPPIV-positive duct-like structures which co-localized with biliary epithelial CD49f.


CONCLUSIONS
Liver IR and PH as a preconditioning stimulus enables multiple cell liver repopulation by donor hepatocytes, LSECs, and bile duct cells.
Krause
Petra
P
Department of General Surgery, University Medical Centre Goettingen, Robert-Koch-Strasse 40, Goettingen, Germany.
Rave-Fränk
Margret
M
Wolff
Hendrik Andreas
HA
Becker
Heinz
H
Christiansen
Hans
H
Koenig
Sarah
S
D000818:Animals; D001652:Bile Ducts / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery*; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D018819:Dipeptidyl Peptidase 4 / Q000172:deficiency / Q000235:genetics; D042783:Endothelial Cells / Q000201:enzymology / Q000637:transplantation*; D006498:Hepatectomy; D022781:Hepatocytes / Q000201:enzymology / Q000637:transplantation*; D008099:Liver / Q000098:blood supply / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery*; D008115:Liver Regeneration / Q000528:radiation effects*; D051381:Rats; D011916:Rats, Inbred F344; D055647:Rats, Transgenic; D013997:Time Factors; D019172:Transplantation Conditioning
D018819:Dipeptidyl Peptidase 4