title: Ethanol-mediated carcinogenesis in the human esophagus implicates CYP2E1 induction and the generation of carcinogenic DNA-lesions.
abstract: Chronic alcohol consumption is a major risk factor for esophageal cancer. Various mechanisms may mediate carcinogenesis including the genotoxic effect of acetaldehyde and oxidative stress. Ethanol exerts its carcinogenic effect in the liver among others via the induction of cytochrome P450 2E1 (CYP2E1) and the generation of carcinogenic etheno-DNA adducts. Here we investigated if such effects can also be observed in the human esophagus. We studied nontumorous esophageal biopsies of 37 patients with upper aerodigestive tract cancer and alcohol consumption of 102.3 ± 131.4 g/day (range: 15-600 g) as well as 16 controls without tumors (12 teetotalers and 4 subjects with a maximum of 25 g ethanol/day). CYP2E1, etheno-DNA adducts and Ki67 as a marker for cell proliferation were determined immunohistologically. Chronic alcohol ingestion resulted in a significant induction of CYP2E1 (p = 0.015) which correlated with the amount of alcohol consumed (r = 0.6, p < 0.001). Furthermore, a significant correlation between CYP2E1 and the generation of the carcinogenic exocyclic etheno-DNA adducts 1,N(6)-ethenodeoxyadenosine (r = 0.93, p < 0.001) and 3,N(4)-ethenodeoxycytidine (r = 0.92, p < 0.001) was observed. Etheno-DNA adducts also correlated significantly with cell proliferation (p < 0.01), which was especially enhanced in patients who both drank and smoked (p < 0.001). Nonsmokers and nondrinkers had the lowest rate of cell proliferation, CYP2E1 expression and DNA lesions. Our data demonstrate for the first time an induction of CYP2E1 in the esophageal mucosa by ethanol in a dose dependent manner in man and may explain, at least in part, the generation of carcinogenic DNA lesions in this target organ.
authors: Department of Medicine, Salem Medical Centre and Centre of Alcohol Research, Liver Disease and Nutrition, University of Heidelberg, Heidelberg, Germany.
authors: Gunda
authors: G
authors: Millonig
authors: Ying
authors: Y
authors: Wang
authors: Nils
authors: N
authors: Homann
authors: Friederike
authors: F
authors: Bernhardt
authors: Hua
authors: H
authors: Qin
authors: Sebastian
authors: S
authors: Mueller
authors: Helmut
authors: H
authors: Bartsch
authors: Helmut K
authors: HK
authors: Seitz
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000235:genetics; D015197:Carcinogenicity Tests; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics* / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism; D004938:Esophageal Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000235:genetics*; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012907:Smoking / Q000453:epidemiology / Q000235:genetics
chemical_list: D018736:DNA Adducts; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1