Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy.
Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.
Kudlinski
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Laboratoire PhLAM, Université Lille 1, IRCICA, 59655 Villeneuve d'Ascq, France. alexandre.kudlinski@univ-lille1.fr
Lelek
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M
Barviau
B
B
Audry
L
L
Mussot
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D003116:Color; D003952:Diagnostic Imaging / Q000295:instrumentation*; D004867:Equipment Design; D005434:Flow Cytometry / Q000379:methods*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D017785:Photons