CTLA4 CT60 A/G gene polymorphism in liver transplant recipients.
OBJECTIVE
Cytotoxic T-lymphocyte antigen 4 (CTLA4) has a critical role in the down-regulation of the immune response. We retrospectively examined the association between acute rejection and the single nucleotide polymorphism A/G in the CTLA-4 CT60 gene in liver transplant recipients.


METHODS
Fifty-one liver transplant recipients with at least 3 months' follow-up were selected and genotyped for CTLA-4 CT60 polymorphism (HpyCH4 IV). The association of each genotype with allograft acute rejection was evaluated.


RESULTS
The mean age of patients was 27.9 +/- 15.17 years (minimum, 1 year, maximum, 55 years), with 39% male and 61% female. Overall, 17 recipients (33.3%) experienced acute rejection within the first 3 months after a liver transplant. In our study, 50% of the patients (n=26) have G/A , 31% (n=16) have A/A, and 17% have G/G genotypes (n=9). Distribution of alleles was not different according to underlying liver disease. There also was no difference in sex, age, and distributions of CTLA-4 CT60 alleles with acute rejection episodes.


CONCLUSIONS
CT60 A/G dimorphism within the 3'-UTR of CTLA4 gene does not influence acute rejection development in liver transplant. However, organ rejection is determined by a combination of several genetic traits rather than a single gene. Therefore, more studies with larger patient numbers are necessary to investigate the effect of combinations of genetic phenotypes involved in this process.
Azarpira
Negar
N
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. negarazarpira@yahoo.com
Malekhosseini
Seyed Ali
SA
Aghdaie
Mahdokht Hosein
MH
Daraie
Masumeh
M
D020413:3' Untranslated Regions; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D060908:CTLA-4 Antigen; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D006085:Graft Survival / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007492:Iran; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D016017:Odds Ratio; D010641:Phenotype; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D055815:Young Adult
D020413:3' Untranslated Regions; D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human