Heligmosomoides polygyrus infection can inhibit colitis through direct interaction with innate immunity.
Less developed countries have a low incidence of immunological diseases like inflammatory bowel disease (IBD), perhaps prevented by the high prevalence of helminth infections in their populations. In the Rag IL-10(-/-) T cell transfer model of colitis, Heligmosomoides polygyrus, an intestinal helminth, prevents and reverses intestinal inflammation. This model of colitis was used to explore the importance of innate immunity in H. polygyrus protection from IBD. Rag mice briefly exposed to H. polygyrus before reconstitution with IL-10(-/-) colitogenic T cells are protected from colitis. Exposure to H. polygyrus before introduction of IL-10(-/-) and OT2 T cells reduced the capacity of the intestinal mucosa to make IFN-gamma and IL-17 after either anti-CD3 mAb or OVA stimulation. This depressed cytokine response was evident even in the absence of colitis, suggesting that the downmodulation in proinflammatory cytokine secretion was not just secondary to improvement in intestinal inflammation. Following H. polygyrus infection, dendritic cells (DCs) from the lamina propria of Rag mice displayed decreased expression of CD80 and CD86, and heightened expression of plasmacytoid dendritic cell Ag-1 and CD40. They were also less responsive to lamina proprias, producing less IL-12p40 and IL-10. Also diminished was their capacity to present OVA to OT2 T cells. These experiments infer that H. polygyrus does not require direct interactions with T or B cells to render animals resistant to colitis. DCs have an important role in driving both murine and human IBD. Data suggest that phenotypic alternations in mucosal DC function are part of the regulatory process.
Hang
Long
L
Division of Gastroenterology-Hepatology, Department of Internal Medicine, Tufts Medical Center, Boston, MA 02111, USA.
Setiawan
Tommy
T
Blum
Arthur M
AM
Urban
Joseph
J
Stoyanoff
Korynn
K
Arihiro
Seiji
S
Reinecker
Hans-Christian
HC
Weinstock
Joel V
JV
D000818:Animals; D002478:Cells, Cultured; D003092:Colitis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control*; D003713:Dendritic Cells / Q000276:immunology / Q000469:parasitology; D004195:Disease Models, Animal; D007113:Immunity, Innate / Q000235:genetics; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009092:Mucous Membrane / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D009351:Nematospiroides dubius / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D017206:Strongylida Infections / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000469:parasitology / Q000637:transplantation
D016753:Interleukin-10; D010047:Ovalbumin