The impact of silica gel pore and particle sizes on HPLC column efficiency and resolution for an immobilized, cyclodextrin-based, chiral stationary phase.
In this study, the CD derivative, mono (6(A)-azido-6(A)-deoxy)-per(p-chlorophenylcarbamonylated) β-CD was chemically immobilized onto the surface of an amino-functionalized silica gel with different pore (100, 300, and 500Å) and particle (3, 5, and 10 μm) sizes to obtain novel chiral stationary phases. The impact of pore and particle size on the amount of immobilized Ph-β-CD, column performance, and enantioselectivity was investigated by evaluating the separation of a variety of racemates in both the normal- and the reversed-phase modes. Experimental results revealed that the retention factor and resolution of racemates generally decreased with increasing pore size; the column prepared with the smallest (3 μm) silica gel particle size gave the best column performance and enantioselectivity in both the normal- and the reversed-phase modes.
Qin
Qing
Q
School of Chemistry and Environment, South China Normal University, Guangzhou, P. R. China.
Zhang
Sheng
S
Zhang
Wei-Guang
WG
Zhang
Zhen-Bin
ZB
Xiong
Ya-Jin
YJ
Guo
Zi-Yuan
ZY
Fan
Jun
J
Run-Zheng
Sheng
S
Finlow
David
D
Yin
Yong
Y
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D008422:Materials Testing; D015394:Molecular Structure; D010316:Particle Size; D016062:Porosity; D058428:Silica Gel / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D047392:beta-Cyclodextrins; D058428:Silica Gel