title: Glycoalkaloid profile in potato haploids derived from solanum tuberosum-S. bulbocastanum somatic hybrids.
abstract: Cultivated and wild potato species synthesize a wide variety of steroidal glycoalkaloids (GA) that may affect either human health or biotic stress resistance. Therefore, GA composition must be a major criterion in the evaluation of breeding products when species genomes are merged and/or manipulated. This work reports the results of GA analysis performed on unique haploid (2n=2x=24) plants obtained from tetraploid (2n=4x=48) Solanum bulbocastanum-S. tuberosum hybrids through in vitro anther culture. Glycoalkaloids were extracted from tubers and analyzed by HPLC. Haploids generally showed the occurrence of parental GA. However, in several cases loss of parental GA and gain of new GA lacking in the parents was observed. It may be hypothesized that new GA profiles of our haploids is the result of either genetic recombination or combinatorial biochemistry events. To highlight differences between haploids and parents, soluble proteins and antioxidant activities were also determined. Both were always higher in haploids compared to their parents. The nature of the newly formed GAs will be further investigated, because they may represent new metabolites that can be used against pest and diseases, or are useful for human health.
authors: Department of Soil, Plant, Environmental and Animal Production Sciences, Via Università 100, I-80055 Portici. carputo@unina.it
authors: Domenico
authors: D
authors: Carputo
authors: Salvatore
authors: S
authors: Savarese
authors: Anna
authors: A
authors: Andolfi
authors: Riccardo
authors: R
authors: Aversano
authors: Alessio
authors: A
authors: Cimmino
authors: Luigi
authors: L
authors: Frusciante
authors: Antonio
authors: A
authors: Evidente
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006238:Haploidy; D006824:Hybridization, Genetic; D012991:Solanaceous Alkaloids / Q000737:chemistry*; D032322:Solanum / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D011198:Solanum tuberosum / Q000737:chemistry* / Q000145:classification / Q000235:genetics
chemical_list: D000975:Antioxidants; D012991:Solanaceous Alkaloids