Kinetic investigation of bioresponsive nanoparticle assembly as a function of ligand design.
Homogeneous and heterogeneous nanoparticle (NP) assembly induced by ligand-specific immunorecognition is commonly used for biosensing applications. We investigated how the structural design of the peptide ligands used to functionalise gold NPs affected the kinetics of NP assembly and hence biodetection. We observed that aggregation rates varied up to 20-fold for the surface binding and 120-fold for the solution-phase assembly of NPs as a function of peptide design. Our results show how the fundamental difference in NP assembly on surfaces and in solution requires different optimised ligand designs. This increased understanding of the specifics of ligand-triggered NP aggregation should help in the design of faster and more efficient bioassays in the future.
Andresen
Heiko
H
Imperial College London, Department of Materials and Institute for Biomedical Engineering, Prince Consort Road, London, SW7 2AZ, UK.
Gupta
Shalini
S
Stevens
Molly M
MM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D008024:Ligands; D010455:Peptides; D006046:Gold