Protein discrimination by fluorescent sensor array constituted of variously charged dendritic phenylene-ethynylene fluorophores.
A protein fluorescent sensor array based on variously charged dendritic fluorophores is developed. The variation of charge is achieved by different combinations of cationic trimethylammonium, anionic carboxylate and non-ionic methyl ester or N,N-dimethylamino on the peripheries of the fluorophores. Nine phenylene-ethynylene dendritic fluorophores, seven zeroth generation bearing charges varied from -3 to +3 and two first generation bearing -6 and +6 charges, are synthesized from triiodotriphenylamine through series of Sonogashira coupling. In phosphate buffer saline pH 7.4, five out of these nine compounds fluorogenically response to eight protein analytes distinctively. The data set of fluorescent intensities obtained from 5 fluorophores×8 proteins×9 replicated measurements can be statistically sorted into eight clusters corresponding to each protein by principal component analysis (PCA). Factorial discriminant analysis (FDA) cross-validation is applied to locate the optimum detection wavelength and reduce the number of sensing elements from nine to two with 100% discriminating accuracy. The method described should be generally practical for the development of electronic tongue for protein related food analysis and medical diagnosis.
Niamnont
Nakorn
N
Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Mungkarndee
Radeemada
R
Techakriengkrai
Ittipon
I
Rashatasakhon
Paitoon
P
Sukwattanasinitt
Mongkol
M
D015374:Biosensing Techniques / Q000295:instrumentation*; D050091:Dendrimers / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D050091:Dendrimers; D005456:Fluorescent Dyes; D011108:Polymers