title: Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.
abstract: Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.
authors: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA.
authors: Seok-Hee
authors: SH
authors: Hyun
authors: Hee-kwon
authors: HK
authors: Kim
authors: Jong-Mok
authors: JM
authors: Kim
authors: David H
authors: DH
authors: Thompson
mesh_terms: D017103:Bacillus Phages; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry*; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D010742:Phospholipid Ethers / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: C480343:2,2'-di-O-decyl-3,3'-O-1,20-eicosanyl-bis-rac-glycero-1,1'-diphosphocholine; D036022:Capsid Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D010742:Phospholipid Ethers; C097975:portal protein, bacteriophage phi29; D006639:Histidine