title: [Resistance/sensitivity in vitro in ovarian cancer patients].
abstract: OBJECTIVE
  To assay resistance/sensitivity by MTT test in solid tumor or ascitic fluid in ovarian cancer patients.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Department of Gynecology and Obstetrics, Medical Faculty Charles University, Prague and University Hospital, Hradec Králové.
  
  
  METHODS
  MTT - (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) chemosensitivity assay was performed in 32 samples of ovarian cancer tissue and 26 samples of ascitic fluid in ovarian cancer patients. We studied the in vitro drug resistance profiles of ovarian cancer specimens exposed to cisplatin, carboplatin, paclitaxel, topotecan, gemcitabin, etoposid.
  
  
  RESULTS
  The highest frequency of resistance in vitro occured for etoposid, gemcitabin and paclitaxel and the most effective chemotherapeutical agents in vitro were cisplatinum and topotecan. Cisplatin had the lowest incidence of drug resistance in vitro than carboplatin.
  
  
  CONCLUSIONS
  Resistance/sensitivity assay would improve the treatment and prognosis of ovarian cancer patients.
authors: Porodnickiá a gynekologická klinika LF UK a FN, Hradec Králové. sedlakiva@seznam.cz
authors: I
authors: I
authors: Sedláková
authors: J
authors: J
authors: Tosner
authors: A
authors: A
authors: Rezác
authors: M
authors: M
authors: Cervinka
authors: K
authors: K
authors: Brigulová
authors: J
authors: J
authors: Spacek
authors: M
authors: M
authors: Tomsová
authors: P
authors: P
authors: Skapinec
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010051:Ovarian Neoplasms / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents