Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin.
Differential scanning calorimetry was used to investigate the thermal unfolding of actin specifically cleaved within the DNaseI-binding loop between residues Met47-Gly48 or Gly42-Val43 by two bacterial proteases, subtilisin or ECP32/grimelysin (ECP), respectively. The results obtained show that both cleavages strongly decreased the thermal stability of monomeric actin with either ATP or ADP as a bound nucleotide. An even more pronounced difference in the thermal stability between the cleaved and intact actin was observed when both actins were polymerized into filaments. Similar to intact F-actin, both cleaved F-actins were significantly stabilized by phalloidin and aluminum fluoride; however, in all cases, the thermal stability of the cleaved F-actins was much lower than that of intact F-actin, and the stability of ECP-cleaved F-actin was lower than that of subtilisin-cleaved F-actin. These results confirm that the DNaseI-binding loop is involved in the stabilization of the actin structure, both in monomers and in the filament subunits, and suggest that the thermal stability of actin depends, at least partially, on the conformation of the nucleotide-binding cleft. Moreover, an additional destabilization of the unstable cleaved actin upon ATP/ADP replacement provides experimental evidence for the highly dynamic actin structure that cannot be simply open or closed, but rather should be considered as being able to adopt multiple conformations.
Pivovarova
Anastasia V
AV
A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Khaitlina
Sofia Yu
SY
Levitsky
Dmitrii I
DI
D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000378:metabolism; D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D017607:Aluminum Compounds / Q000378:metabolism; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002413:Cations, Divalent / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism*; D005459:Fluorides / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects; D008024:Ligands; D010590:Phalloidine / Q000378:metabolism; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability; D058767:Protein Unfolding; D011817:Rabbits; D013379:Substrate Specificity; D020860:Subtilisin / Q000378:metabolism*; D044366:Transition Temperature
D000199:Actins; D017607:Aluminum Compounds; D002413:Cations, Divalent; D008024:Ligands; D010590:Phalloidine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010450:Endopeptidases; C097881:proteinase ECP 32; D020860:Subtilisin; D005459:Fluorides; C032311:aluminum fluoride