[Erythropoietin gene-modified conditioned medium of human mesenchymal cells promotes hematopoietic development from human embryonic stem cells].
The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells.
Yang
Chao
C
Laboratory of Stem Cell and Regenerative Medicine, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
Ji
Lei
L
Yue
Wen
W
Wang
Ruo-Yong
RY
Li
Yan-Hua
YH
Xi
Jia-Fei
JF
Xie
Xiao-Yan
XY
He
Li-Juan
LJ
Nan
Xue
X
Pei
Xue-Tao
XT
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D004921:Erythropoietin / Q000235:genetics / Q000494:pharmacology*; D006413:Hematopoietic System; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D030781:Organisms, Genetically Modified
D017077:Culture Media, Conditioned; D004921:Erythropoietin