title: Luciferase and green fluorescent protein reporter genes as tools to determine protein abundance and intracellular dynamics.
abstract: To get insight into molecular mechanisms governing plant development, the dynamics of abundance and cellular localisation of signalling components need to be understood. Luciferase and green fluorescent protein (GFP)-derived reporters are suitable markers to determine dynamic signalling processes in vivo. Here, analysis of phytochrome A (phyA) photoreceptor dynamics during early seedling development is used as an example of how in vitro and in vivo luciferase assays as well as GFP-imaging can be used to probe signalling dynamics.
authors: Institute of Plant Biology, Hungarian Academy of Science, Biological Research Center, Szeged, Hungary.
authors: András
authors: A
authors: Viczián
authors: Stefan
authors: S
authors: Kircher
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D008156:Luciferases / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods; D055519:Photoreceptors, Plant / Q000235:genetics* / Q000378:metabolism; D051942:Phytochrome A / Q000235:genetics* / Q000378:metabolism; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D055519:Photoreceptors, Plant; D051942:Phytochrome A; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases