Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation.
The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs production of flavonol glycoside1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs. A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling. Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds. The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.
Stracke
Ralf
R
Department of Biology, Bielefeld University, Genome Research, D-33594 Bielefeld, Germany. ralf.stracke@uni-bielefeld.de
Jahns
Oliver
O
Keck
Matthias
M
Tohge
Takayuki
T
Niehaus
Karsten
K
Fernie
Alisdair R
AR
Weisshaar
Bernd
B
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D044948:Flavonols / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics; D014453:Glucuronosyltransferase / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D011058:Pollen / Q000166:cytology / Q000378:metabolism; D013194:Staining and Labeling; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D029681:Arabidopsis Proteins; D044948:Flavonols; D006027:Glycosides; C529650:MYB11 protein, Arabidopsis; C504539:MYB12 protein, Arabidopsis; D014157:Transcription Factors; D014453:Glucuronosyltransferase