[Role of nuclear factor kappa B in cyclosporine A-induced nephrotoxicity in human tubular epithelial cell].
OBJECTIVE
To ascertain the role of FK-kappaB in cyclosporine A (CsA)-induced nephrotoxicity in human kidney tubular epithelial cell.


METHODS
Human kidney tubular epithelial cells were treated with CsA at concentrations of 0, 0.1, 1, 10 mmol/L for 24 hours. As inhibitors of NF-kappaB, 25 micromol/L pyrrolidine dithiocarbamate (PDTC) were added respectively. Mitochondria membrane potential were detected by flow cytometry. The activation of NF-kappaB was studied by the assessment of NF-kappaB P65 measured by laser scanning confocal microscope.


RESULTS
Mitochondrial membrane potential showed a decrease in the cells with CsA, but the cells with CsA + PDTC did not. Cells treated with CsA activated NF-kappaB but cells in the CsA-induced damage of human kidney tubular epithelial cell. This damage is ameliorated when the activation of NF-kappaB is blockaded.
Shu
Jun
J
Guangdong General Hospital, Guangzhou 510080, China. junshu020@hotmail.com
Zhou
Jian-ping
JP
D002460:Cell Line; D016572:Cyclosporine / Q000009:adverse effects*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism*
D016328:NF-kappa B; D016572:Cyclosporine