title: Possible role of vitamin E, coenzyme Q10 and rutin in protection against cerebral ischemia/reperfusion injury in irradiated rats.
abstract: OBJECTIVE
  To investigate the possible role of vitamin E, coenzyme Q10 and rutin in ameliorating the biochemical changes in brain and serum induced by cerebral ischemia/reperfusion (I/R) in whole body γ-irradiated rats.
  
  
  METHODS
  Cerebral ischemia was induced in male Wistar rats (either irradiated or non-irradiated) followed by reperfusion.
  
  
  RESULTS
  I/R increased brain content of malondialdehyde (MDA) and depleted its glutathione (GSH) content with a compensatory elevation in cytosolic activities of glutathione peroxidase (GPx) and glutathione reductase (GR) enzymes. It also raised brain cytosolic lactate dehydrogenase (LDH) activity and calcium (Ca(2+)) level. Furthermore, I/R provoked an inflammatory response reflected by an increment in serum levels of the proinflammatory cytokines tumour necrosis factor-α (TNF-α) and interlukin-1β (IL-1β). Moreover, induction of I/R in irradiated rats resulted in a further increase in brain oxidative stress and cytosolic LDH activity, disturbed brain Ca(2+) homeostasis and exaggerated the inflammatory reaction. During irradiation, administration of each of vitamin E, coenzyme Q10 (CoQ10) and rutin to irradiated rats before induction of I/R, alleviated the brain oxidative stress. Moreover, these antioxidants caused attenuation of the rise of the cytosolic activities of GPx and GR. A lowering effect of the cytosolic LDH activity and Ca(2+) level were caused by treatment with antioxidants. Each of vitamin E and rutin revealed an anti-inflammatory action of these antioxidants, while CoQ10 had no effect on serum levels of TNF-α and IL-1β.
  
  
  CONCLUSIONS
  These findings indicate that supplementation with either vitamin E, CoQ10 or rutin ameliorated most of the biochemical changes induced by I/R in irradiated rat brain and serum.
authors: Drug Radiation Research Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt.
authors: Amal A
authors: AA
authors: Abd-El-Fattah
authors: Maha M
authors: MM
authors: El-Sawalhi
authors: Engy R
authors: ER
authors: Rashed
authors: Mona A
authors: MA
authors: El-Ghazaly
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002545:Brain Ischemia / Q000188:drug therapy* / Q000209:etiology* / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D012431:Rutin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D053583:Interleukin-1beta; D011837:Radiation-Protective Agents; D014409:Tumor Necrosis Factor-alpha; D014451:Ubiquinone; D014810:Vitamin E; D008315:Malondialdehyde; D012431:Rutin; C024989:coenzyme Q10; D005978:Glutathione