title: Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development.
abstract: LIM mineralization protein 1 (LMP-1) is an essential positive regulator of osteoblast differentiation, maturation and bone formation. Our previous investigations on the distribution of LMP-1 in mature human teeth indicated that LMP-1 might play a role in the odontoblast differentiation and dentin matrix mineralization. The aim of the present study was to use immunohistochemistry to determine the expression of LMP-1 during tooth development in mouse molars. In embryonic and postnatal Kunming mice, LMP-1 protein was expressed during molar development, but the expression levels and patterns differed at various developmental stages. At embryonic day 13.5 (E13.5), LMP-1 was found in the enamel organ. At E14.5, LMP-1 was detected in the entire enamel organ and in the underlying mesenchyme. At E16.5, LMP-1 was observed in the inner and outer enamel epithelium and the stratum intermedium. The expression also converged at the cusps in the dental papilla. At E18.5 and postnatal day 2.5 (P2.5), LMP-1 was restricted to the stratum intermedium, in differentiating dental papilla cells at cusps, while it disappeared in terminal differentiated ameloblasts and odontoblasts. At P13.5, no positive staining was detected in the odontoblasts or in the dental pulp cells. Therefore, LMP-1 showed spatiotemporal expression patterns during molar development and might participate in molar crown morphogenesis and odontoblast differentiation at late molar development.
authors: Hubei-MOST KLOS and KLOBM, School and Hospital of Stomatology, Wuhan University, 430079 Wuhan, Hubei, People's Republic of China.
authors: Pingjuan
authors: P
authors: Fang
authors: Xiaoying
authors: X
authors: Wang
authors: Lu
authors: L
authors: Zhang
authors: Guohua
authors: G
authors: Yuan
authors: Zhi
authors: Z
authors: Chen
authors: Qi
authors: Q
authors: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003598:Cytoskeletal Proteins; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D060588:LIM Domain Proteins; D051379:Mice; D008963:Molar / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D021381:Protein Transport; D014083:Tooth Germ / Q000166:cytology / Q000196:embryology / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C484381:Pdlim7 protein, mouse