title: [A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi].
abstract: OBJECTIVE
  To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE, CRF07_BC, CRF08_BC, B, and C strains prevailing in Guangxi.
  
  
  METHODS
  Subtype-specific primers were designed for these subtypes based on their gag sequences. The subtypes of HIV-1 samples from Guangxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis, respectively, and then the sensitivity and the specificity of nested multiplex PCR were calculated.
  
  
  RESULTS
  Nested multiplex PCR could correctly classify the 5 known-subtype samples, and were not reactive to all HIV-negative samples. Of the 72 HIV-positive samples, 66 were correctly identified as CRF01_AE, CRF07_BC, CRF08_BC, and B by this assay, giving a sensitivity of 91.7% (66/72), and a specificity of 100%.
  
  
  CONCLUSIONS
  This assay is a simple, fast, and cost-effective subtyping method for HIV-1 CRF01-AE, CRF07_BC, CRF08_BC, and B strains prevailing in Guangxi.
authors: Center for AIDS Research & School of Public Health, Guangxi Medical University, Nanning, China.
authors: Qi-jian
authors: QJ
authors: Su
authors: Ping
authors: P
authors: Zhou
authors: Zhi-you
authors: ZY
authors: Bi
authors: Xin
authors: X
authors: Xiao
authors: Shu-zhi
authors: SZ
authors: Wu
authors: Ping
authors: P
authors: Cen
authors: Wei
authors: W
authors: Deng
authors: Yi-ming
authors: YM
authors: Shao
authors: Hao
authors: H
authors: Liang
mesh_terms: D002681:China; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*
chemical_list: D017931:DNA Primers