title: Association between anal sac gland carcinoma and dog leukocyte antigen-DQB1 in the English Cocker Spaniel.
abstract: Anal sac gland carcinomas occur frequently in English Cocker Spaniels and, to a lesser extent, in other spaniel breeds. The disease typically presents in dogs aged 8 years or older and frequently metastasises to the local lymph nodes. The association between anal sac gland carcinoma in English Cocker Spaniels and the major histocompatibility complex class II loci (the dog leukocyte antigen loci DLA-DRB1, -DQA1, -DQB1) was investigated in 42 cases and 75 controls. Based on a corrected error rate of 0.017 for each test, the allele distribution in DLA-DRB1 showed no significant difference between cases and controls (P value = 0.019), while a significant difference was obtained for DLA-DQA1 and -DQB1 alleles (P values are 0.010 and 3.3 × 10⁻⁵). The DLA-DQB1*00701 allele was the most common in both cases and controls, but it had a higher frequency among the former (0.89) than in the latter (0.61), while the second most common allele had a higher frequency in the controls (0.23) than in the cases (0.07). Haplotype distributions were also significantly different between the two groups (P value = 1.61 × 10⁻⁴). This is the second disease in English Cocker Spaniels for which the most common DLA-DQB1 allele in the breed has been shown to have a higher frequency in cases than controls, while the second most common allele in the breed (*02001) has a significantly higher frequency in the controls, compared with the cases.
authors: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK. ja248@cam.ac.uk
authors: J
authors: J
authors: Aguirre-Hernández
authors: G
authors: G
authors: Polton
authors: L J
authors: LJ
authors: Kennedy
authors: D R
authors: DR
authors: Sargan
mesh_terms: D000483:Alleles; D000818:Animals; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D005260:Female; D005787:Gene Frequency / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008297:Male; D009369:Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000662:veterinary*
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II