Modelling acute renal failure using blood and breath biomarkers in rats.
This paper compares three methods for estimating renal function, as tested in rats. Acute renal failure (ARF) was induced via a 60-min bilateral renal artery clamp in 8 Sprague-Dawley rats and renal function was monitored for 1 week post-surgery. A two-compartment model was developed for estimating glomerular filtration via a bolus injection of a radio-labelled inulin tracer, and was compared with an estimated creatinine clearance method, modified using the Cockcroft-Gault equation for rats. These two methods were compared with selected ion flow tube-mass spectrometry (SIFT-MS) monitoring of breath analytes. Determination of renal function via SIFT-MS is desirable since results are available non-invasively and in real time. Relative decreases in renal function show very good correlation between all 3 methods (R²=0.84, 0.91 and 0.72 for breath-inulin, inulin-creatinine, and breath-creatinine correlations, respectively), and indicate good promise for fast, non-invasive determination of renal function via breath testing.
Moorhead
Katherine T
KT
Department of Mechanical Engineering, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand. ktm19@student.canterbury.ac.nz
Hill
Jonathan V
JV
Chase
J Geoffrey
JG
Hann
Christopher E
CE
Scotter
Jennifer M
JM
Storer
Malina K
MK
Endre
Zoltan H
ZH
D058186:Acute Kidney Injury / Q000503:physiopathology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D005919:Glomerular Filtration Rate; D013058:Mass Spectrometry; D008962:Models, Theoretical; D051381:Rats
D015415:Biomarkers