title: Site-specific investigation of the steady-state kinetics and dynamics of the multistep binding of bile acid molecules to a lipid carrier protein.
abstract: The investigation of multi-site ligand-protein binding and multi-step mechanisms is highly demanding. In this work, advanced NMR methodologies such as 2D (1)H-(15)N line-shape analysis, which allows a reliable investigation of ligand binding occurring on micro- to millisecond timescales, have been extended to model a two-step binding mechanism. The molecular recognition and complex uptake mechanism of two bile salt molecules by lipid carriers is an interesting example that shows that protein dynamics has the potential to modulate the macromolecule-ligand encounter. Kinetic analysis supports a conformational selection model as the initial recognition process in which the dynamics observed in the apo form is essential for ligand uptake, leading to conformations with improved access to the binding cavity. Subsequent multi-step events could be modelled, for several residues, with a two-step binding mechanism. The protein in the ligand-bound state still exhibits a conformational rearrangement that occurs on a very slow timescale, as observed for other proteins of the family. A global mechanism suggesting how bile acids access the macromolecular cavity is thus proposed.
authors: Laboratorio NMR, ISMAC-CNR, Via Bassini 15, 20133 Milano, Italy.
authors: Clelia
authors: C
authors: Cogliati
authors: Laura
authors: L
authors: Ragona
authors: Mariapina
authors: M
authors: D'Onofrio
authors: Ulrich
authors: U
authors: Günther
authors: Sara
authors: S
authors: Whittaker
authors: Christian
authors: C
authors: Ludwig
authors: Simona
authors: S
authors: Tomaselli
authors: Michael
authors: M
authors: Assfalg
authors: Henriette
authors: H
authors: Molinari
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular; D012984:Software
chemical_list: D001647:Bile Acids and Salts; D002352:Carrier Proteins