Bioconversion of L-arabinose and other carbohydrates from plant cell walls to alpha-glucan by a soil bacterium, Sporosarcina sp. N52.
A Gram-positive bacterium, N52, that produces intracellular glucan from l-arabinose, was isolated from soil and identified as Sporosarcina sp. according to rRNA gene sequence analysis and physiological/biochemical characterizations. Glucan production by N52 increased significantly in the exponential phase of aerobic liquid culture and was maintained at the highest level during the stationary phase, reaching 37.0% of the cell dry weight. The glucan was also produced from other tested sugars originating from plant cell walls and was composed exclusively of alpha-1,4- and alpha-1,6-glucosidic linkages. When distillery waste was treated with N52 for 72 h, the total organic carbon (TOC), chemical oxygen demand and biochemical oxygen demand were reduced by 42.6%, 45.9% and 82.5%, respectively. Bacterial cells accumulated 31.9% of glucan per cell dry weight, fixing 16.0% of the TOC in the soluble fraction. Thus, this strain could provide us with a new process for waste management, including the bioconversion of organic materials to the valuable byproduct, alpha-glucan.
Zhang
Zilian
Z
Food Resource Division, National Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Srichuwong
Sathaporn
S
Kobayashi
Tooru
T
Arakane
Mitsuhiro
M
Park
Jeung-Yil
JY
Tokuyasu
Ken
K
D001089:Arabinose / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D057168:Distillation; D005936:Glucans / Q000096:biosynthesis*; D006027:Glycosides / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D058644:Sporosarcina / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis
D005936:Glucans; D006027:Glycosides; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D002244:Carbon; D001089:Arabinose