title: Dietary broccoli sprouts protect against myocardial oxidative damage and cell death during ischemia-reperfusion.
abstract: Cruciferous vegetables are known for antioxidant and anti-carcinogenic effects. In the current study we asked whether dietary broccoli sprouts can protect the heart from ischemia-reperfusion. Rats were fed either control diet (sham and control groups) or a diet mixed with 2% dried broccoli sprouts for 10 days. After 10 days the isolated hearts were subjected to ischemia for 20 min and reperfusion for 2 h, and evaluated for cell death, oxidative damage, and Nrf2-regulated phase 2 enzyme activities. Broccoli sprouts feeding inhibited markers of necrosis (lactate dehydrogenase release) and apoptosis (caspase-3 activity) by 78-86%, and decreased indices of oxidative stress (thiobarbituric acid reactive substances and aconitase inactivation) by 82-116%. While broccoli sprouts increased total glutathione and activities of the phase 2 enzymes glutamate cysteine ligase and quinone reductase in liver, they did not affect these in ischemic-reperfused heart. While the mechanism is not clear, the results show that a relatively short dietary treatment with broccoli sprouts can strongly protect the heart against oxidative stress and cell death caused by ischemia-reperfusion.
authors: College of Health and Nutrition, Shiraz University of Medical Sciences, PO Box 71645-111, Shiraz, Iran. msm.akhlaghi@gmail.com
authors: Masoumeh
authors: M
authors: Akhlaghi
authors: Brian
authors: B
authors: Bandy
mesh_terms: D000154:Aconitate Hydratase / Q000037:antagonists & inhibitors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001937:Brassica; D053148:Caspase 3 / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D050217:Metabolic Detoxication, Phase II; D017202:Myocardial Ischemia / Q000178:diet therapy* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D009336:Necrosis / Q000517:prevention & control; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use*; D018520:Plant Shoots; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D000975:Antioxidants; D028321:Plant Preparations; D017392:Thiobarbituric Acid Reactive Substances; D016660:NAD(P)H Dehydrogenase (Quinone); D053148:Caspase 3; D000154:Aconitate Hydratase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione