title: [Preoperative conjunctival bacterial microbiota of antiangiogenic intravitreous injection for age-related macular degeneration compared to cataract surgery preoperative microbiota].
abstract: OBJECTIVE
  To evaluate the conjunctival bacterial microbiota and antibiogram profile in the preoperative of antiangiogenic intravitreous injection for age-related macular degeneration, and compare to the preoperative microbiota of patients submitted to cataract surgery.
  
  
  METHODS
  Cross-sectional, observational, case series study. Two groups were organized: group I (macular degeneration) with 26 eyes from 26 patients (12 men/14 women) with mean age of 69.2 +/- 11.5 years; group II (cataract) with 27 eyes from 27 patients (9 men/18 women) with mean age of 67.6 +/- 7.9 years. The groups were similar regarding age (p=0.538) and gender (p=0.787). The lower conjunctival sac was swabbed and the obtained material was immediately put in a tube filled with liquid culture media BHI ("brain heart infusion"). Samples were processed according to standard laboratory techniques and antibiogram was determined for each bacterial colony.
  
  
  RESULTS
  Twenty-six bacterial colonies growth in group I, with 2 eyes showing no growth and 30 colonies growth in group II. Gram positive bacteria were more prevalent in both groups: 23/26 colonies (88.4%) in group I and 29/30 colonies (96.7%) in group II, with a Staphylococcus aureus predominance in both groups, with 16 samples (61.5%) and 17 (56.7%), respectively. Coagulase negative Staphylococcus was the second most common identified bacteria, with 19.2% in group I and 20.0% in group II. No differences between the groups reached statistical significance. No statistically significant difference was noted on the antibiotic sensibility between both groups.
  
  
  CONCLUSIONS
  There was no difference in the distribution of bacteria and antibiogram profile of the conjunctival microbiota in the preoperative of intravitreous injection of antiangiogenic for macular degeneration compared to the preoperative of cataract surgery.
authors: Centro de Ciências da Saúde, Universidade Federal de Pernambuco, Recife, PE, Brasil. jrpdiniz@hotmail.com
authors: José Ricardo
authors: JR
authors: Diniz
authors: Kléper Carvalho Figueiredo
authors: KC
authors: Leitão
authors: Marcos Eugênio Moraes Nunes de
authors: ME
authors: Souza
authors: Maria de Fátima Alves
authors: Mde F
authors: Diniz
authors: Judith Advincula
authors: JA
authors: Rocha
authors: Marcus Augusto Gomes de
authors: MA
authors: Matos
authors: Célia Maria Machado Barbosa de
authors: CM
authors: Castro
authors: Carlos Teixeira
authors: CT
authors: Brandt
mesh_terms: D000368:Aged; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002387:Cataract Extraction; D015169:Colony Count, Microbial; D003228:Conjunctiva / Q000382:microbiology*; D003430:Cross-Sectional Studies; D005260:Female; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D006801:Humans; D008268:Macular Degeneration / Q000188:drug therapy* / Q000382:microbiology*; D008297:Male; D008826:Microbial Sensitivity Tests; D011300:Preoperative Care
chemical_list: D020533:Angiogenesis Inhibitors; D000900:Anti-Bacterial Agents