Fluorescent receptor-immobilized silica-coated magnetic nanoparticles as a general binding agent for histidine-tagged proteins.
The histidine-tagged protein binding capacity and purification efficiencies of TSMNPs (terpyridine receptor-immobilized silica-coated magnetic nanoparticles) is described.
Cho
Eun Jin
EJ
Department of Wood Science and Landscape Architecture, BK21 Program, Bio-energy Research Institute, Chonnam National University, Gwangju, 500-757, Korea.
Jung
Sera
S
Lee
Kwangho
K
Lee
Hong Joo
HJ
Nam
Kye Chun
KC
Bae
Hyeun-Jong
HJ
D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011725:Pyridines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification
D011506:Proteins; D011725:Pyridines; D006639:Histidine; D012822:Silicon Dioxide; D001617:beta-Glucosidase; C023666:pyridine