Induction of growth arrest by miR-542-3p that targets survivin.
Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3'-untranslated region (3'-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy.
Yoon
Sena
S
Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea.
Choi
Young-Chul
YC
Lee
Suman
S
Jeong
Yongsu
Y
Yoon
Jaeseung
J
Baek
Kwanghee
K
D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D000077022:Survivin