Proteomic analysis of dental tissue microsamples.
Improved understanding of dental enamel development will benefit not only dentistry but also biomedicine more generally. Rat and mouse models of enamel development are relatively well characterized and experimentally powerful. However, the diminutive size of murine teeth makes them difficult to study using standard proteomic approaches. Here we describe gel-based proteomic methods that enable parallel quantification, identification, and functional characterization of proteins from developing rat and mouse teeth. These refined methods are also likely to be applicable to other scarce samples.
Mangum
Jonathan E
JE
Department of Pharmacology, University of Melbourne, Melbourne, Australia.
Kon
Jew C
JC
Hubbard
Michael J
MJ
D000818:Animals; D003743:Dental Enamel / Q000166:cytology / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000378:metabolism; D004586:Electrophoresis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D051379:Mice; D040901:Proteomics / Q000379:methods*; D051381:Rats
D003746:Dental Enamel Proteins; C109069:enamel matrix proteins