The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput.
Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.
Kellie
John F
JF
Technology Development Team, Center for Top Down Proteomics, University of Illinois at Urbana-Champaign, USA.
Tran
John C
JC
Lee
Ji Eun
JE
Ahlf
Dorothy R
DR
Thomas
Haylee M
HM
Ntai
Ioanna
I
Catherman
Adam D
AD
Durbin
Kenneth R
KR
Zamdborg
Leonid
L
Vellaichamy
Adaikkalam
A
Thomas
Paul M
PM
Kelleher
Neil L
NL
D015415:Biomarkers / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D015415:Biomarkers; D011506:Proteins