A continuous-flow, microfluidic fraction collection device.
A microfluidic device is presented that performs electrophoretic separation coupled with fraction collection. Effluent from the 3.5 cm separation channel was focused via two sheath flow channels into one of seven collection channels. By holding the collection channels at ground potential and varying the voltage ratio at the two sheath flow channels, the separation effluent was directed to either specific collection channels, or could be swept past all channels in a defined time period. As the sum of the voltages applied to the two sheath flow channels was constant, the electric field remained at 275 V/cm during the separation regardless of the collection channel used. The constant potential in the separation channel allowed uninterrupted separation for late-migrating peaks while early-migrating peaks were being collected. To minimize the potential for carryover between fractions, the device geometry was optimized using a three-level factorial model. The optimum conditions were a 22.5° angle between the sheath flow channels and the separation channel, and a 350 μm length of channel between the separation outlet and the fraction channels. Using these optimized dimensions, the device performance was evaluated by separation and fraction collection of a fluorescently-labeled amino acid mixture. The ability to fraction collect on a microfluidic platform will be especially useful during automated or continuous operation of these devices or to collect precious samples.
Baker
Christopher A
CA
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, 32306, USA.
Roper
Michael G
MG
D000596:Amino Acids / Q000302:isolation & purification; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D016650:Fluorescein-5-isothiocyanate