Identification and characterization of phage-displayed peptide mimetics of Neisseria meningitidis serogroup B capsular polysaccharide.
Neisseria meningitidis causes meningitis and septicemia. There is no single vaccine against all serogroup B meningococcal (MenB) strains up to now. Their capsular polysaccharide (MenB CPS) bears epitopes both cross-reacting and non-cross-reactive with human polysialic acid. A bactericidal and protective antibody mAb (13D9) recognizing a unique epitope in MenB CPS was used to screen a phage-displayed peptide library. Four peptides, able to bind mAb 13D9 in competition with MenB CPS, were identified. Immunization of mice with the phage-displayed peptides elicited anti-peptide IgG antibodies, mainly IgG(2a) for 3 of the peptides and bactericidal and protective antibody levels for one of them. Peptides specifically targeting the immune response toward epitopes found only in MenB CPS could be considered for a universal vaccine against serogroup B meningococcal strains.
Menéndez
Tamara
T
Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba. tamara.menendez@cigb.edu.cu
Santiago-Vispo
Nelson F
NF
Cruz-Leal
Yoelys
Y
Coizeau
Edelgis
E
Garay
Hilda
H
Reyes
Osvaldo
O
Batista
Yaima
Y
Cobas
Karem
K
Carmenate
Tania
T
Chinea
Glay
G
Guillén
Gerardo
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D022401:Meningococcal Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050296:Microbial Viability; D009345:Neisseria meningitidis / Q000276:immunology*; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D051381:Rats; D058248:Serum Bactericidal Antibody Assay
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines; D019151:Peptide Library; D010455:Peptides; D011135:Polysaccharides, Bacterial