Suppression of anoikis by collagen coating of interconnected macroporous nanometric carbonated hydroxyapatite/agarose scaffolds.
Three dimensional interconnected macroporous (pore diameter: 600-800 μm) hydroxyapatite/agarose disks have been evaluated in this study as potential bone regeneration scaffolds. With this purpose, the adhesion and proliferation of human Saos-2 osteoblasts on this biomaterial were analyzed. As an index of cell function, the following parameters were measured: cell morphology, viability, cell size/complexity, cell cycle, reactive oxygen species (ROS) content, and lactate dehydrogenase (LDH) release. The existence of anoikis induced by inappropriate contacts between the cell and the scaffold has been detected by scanning electron microscopy, confocal microscopy, and flow cytometry. The intracellular nitric oxide content has been also measured as potential inducer of anoikis. The positive effects of previous scaffold coating with type I collagen on osteoblast adhesion as well as the collagen protection against anoikis have been demonstrated in this study.
Alcaide
María
M
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040-Madrid, Spain.
Serrano
María-Concepción
MC
Roman
Jesús
J
Cabañas
María-Victoria
MV
Peña
Juan
J
Sánchez-Zapardiel
Elena
E
Vallet-Regí
María
M
Portolés
María-Teresa
MT
D023102:Anoikis / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002254:Carbonates / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D012685:Sepharose / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D002254:Carbonates; D003094:Collagen; D012685:Sepharose; D017886:Durapatite