Codon-improved fluorescent proteins in investigation of Staphylococcus aureus host pathogen interactions.
Here we present the use of three fluorescent proteins in Staphylococcus aureus, Cerulean, PA-GFP, and mRFPmars. All molecules have an improved codon adaptation for expression in the A + T rich organisms and extend the fluorescent protein portfolio in staphylococcal research.
Paprotka
Kerstin
K
FunGene, Competence Center for Functional Genomics, Ernst-Moritz-Arndt University, F.-L.-Jahnstrasse 15, D-17487 Greifswald, Germany.
Giese
Bernd
B
Fraunholz
Martin J
MJ
D002460:Cell Line; D003062:Codon; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000502:physiology
D003062:Codon; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins