title: Undifferentiated embryonic cell transcription factor 1 regulates ESC chromatin organization and gene expression.
abstract: Previous reports showed that embryonic stem (ES) cells contain hyperdynamic and globally transcribed chromatin-properties that are important for ES cell pluripotency and differentiation. Here, we demonstrate a role for undifferentiated embryonic cell transcription factor 1 (UTF1) in regulating ES cell chromatin structure. Using chromatin immunoprecipitation-on-chip analysis, we identified >1,700 UTF1 target genes that significantly overlap with previously identified Nanog, Oct4, Klf-4, c-Myc, and Rex1 targets. Gene expression profiling showed that UTF1 knock down results in increased expression of a large set of genes, including a significant number of UTF1 targets. UTF1 knock down (KD) ES cells are, irrespective of the increased expression of several self-renewal genes, Leukemia inhibitory factor (LIF) dependent. However, UTF1 KD ES cells are perturbed in their differentiation in response to dimethyl sulfoxide (DMSO) or after LIF withdrawal and display increased colony formation. UTF1 KD ES cells display extensive chromatin decondensation, reflected by a dramatic increase in nucleosome release on micrococcal nuclease (MNase) treatment and enhanced MNase sensitivity of UTF1 target genes in UTF1 KD ES cells. Summarizing, our data show that UTF1 is a key chromatin component in ES cells, preventing ES cell chromatin decondensation, and aberrant gene expression; both essential for proper initiation of lineage-specific differentiation of ES cells.
authors: Department of Developmental Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
authors: Susanne M
authors: SM
authors: Kooistra
authors: Vincent
authors: V
authors: van den Boom
authors: Rajkumar P
authors: RP
authors: Thummer
authors: Frank
authors: F
authors: Johannes
authors: René
authors: R
authors: Wardenaar
authors: Bruno M
authors: BM
authors: Tesson
authors: Liesbeth M
authors: LM
authors: Veenhoff
authors: Fabrizia
authors: F
authors: Fusetti
authors: Laura P
authors: LP
authors: O'Neill
authors: Bryan M
authors: BM
authors: Turner
authors: Gerald
authors: G
authors: de Haan
authors: Bart J L
authors: BJ
authors: Eggen
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics* / Q000502:physiology; D055785:Gene Knockdown Techniques; D051379:Mice; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D015534:Trans-Activators; C112198:Utf1 protein, mouse