Targeted identification of genomic regions using TAGdb.
BACKGROUND
The introduction of second generation sequencing technology has enabled the cost effective sequencing of genomes and the identification of large numbers of genes and gene promoters. However, the assembly of DNA sequences to create a representation of the complete genome sequence remains costly, especially for the larger and more complex plant genomes.


RESULTS
We have developed an online database, TAGdb, that enables researchers to identify paired read sequences that share identity with a submitted query sequence. These tags can be used to design oligonucleotide primers for the PCR amplification of the region in the target genome.


CONCLUSIONS
The ability to produce large numbers of paired read genome tags using second generation sequencing provides a cost effective method for the identification of genes and promoters in large, complex or orphan species without the need for whole genome assembly.
Marshall
Daniel J
DJ
University of Queensland, Australian Centre for Plant Functional Genomics, School of Land, Crop and Food Sciences, Brisbane, QLD 4067, Australia. dave.edwards@uq.edu.au.
Hayward
Alice
A
Eales
Dominic
D
Imelfort
Michael
M
Stiller
Jiri
J
Berkman
Paul J
PJ
Clark
Terry
T
McKenzie
Megan
M
Lai
Kaitao
K
Duran
Chris
C
Batley
Jacqueline
J
Edwards
David
D