title: False negative results in high viremia parvovirus B19-samples tested with real-time PCR.
abstract: Extremely high viremia is observed during some viruses infection, especialy in immunocompromised patients. False negative results of Parvovirus B 19 DNA tests performed with real-time PCR in high viremic samples are reported. The way of fluorescence diagrams analysis and algorithm of positive result confirmation to exclude such phenomenon are proposed.
authors: Department of Immunohaematology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland. pgrabarczyk@ihit.waw.pl
authors: Piotr
authors: P
authors: Grabarczyk
authors: Aleksandra
authors: A
authors: Kalińska
authors: Ewa
authors: E
authors: Sulkowska
authors: Ewa
authors: E
authors: Brojer
mesh_terms: D005188:False Negative Reactions; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D014766:Viremia / Q000175:diagnosis* / Q000821:virology