Fluorescent magnetic nanoprobes: design and application for cell imaging.
Multifunctional nanoprobes combining magnetic nanoparticles with organic dyes have attracted tremendous interest due to their promising applications in biomedical field. Here we demonstrate a facile and general strategy for the fabrication of robust fluorescent magnetic nanoprobes with high payloads of dye molecules and their use as multimodal nanoprobes for cell imaging. These nanoprobes not only effectively keep photochemical stability of dyes, but also provide a platform for grafting other functional or targeted moieties into silica surface via primary amines. Moreover, the nanoprobes are uniformly spherical morphology and can be dispersed well in aqueous solution, which are very desirable for biomedical applications. Importantly, this method can be extended to synthesize other bifunctional nanoprobes by using the dyes with isothiocyanate group.
Zhang
Guo
G
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Feng
Jianghua
J
Lu
Lehui
L
Zhang
Baohua
B
Cao
Linyuan
L
D000588:Amines / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014018:Tissue Distribution
D000588:Amines; D005456:Fluorescent Dyes; D015335:Molecular Probes; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide