title: Functional integration of new neurons into hippocampal networks and poststroke comorbidities following neonatal stroke in mice.
abstract: Stroke in the developing brain is an important cause of chronic neurological morbidities including neurobehavioral dysfunction and epilepsy. Here, we describe a mouse model of neonatal stroke resulting from unilateral carotid ligation that results in acute seizures, long-term hyperactivity, spontaneous lateralized circling behavior, impaired cognitive function, and epilepsy. Exploration-dependent induction of the immediate early gene Arc (activity-regulated cytoskeleton associated protein) in hippocampal neurons was examined in the general population of neurons versus neurons that were generated approximately 1 week after the ischemic insult and labeled with bromodeoxyuridine. Although Arc was inducible in a network-specific manner after severe neonatal stroke, it was impaired, not only in the ipsilateral injured but also in the contralateral uninjured hippocampi when examined 6 months after the neonatal stroke. Severity of both the stroke injury and the acquired poststroke epilepsy negatively correlated with Arc induction and new neuron integration into functional circuits in the injured hippocampi.
authors: Department of Neurology and Developmental Medicine, Kennedy Krieger Research Institute, Baltimore, MD, USA. Kadam@kennedykrieger.org
authors: S D
authors: SD
authors: Kadam
authors: C L
authors: CL
authors: Smith-Hicks
authors: D R
authors: DR
authors: Smith
authors: P F
authors: PF
authors: Worley
authors: A M
authors: AM
authors: Comi
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D004827:Epilepsy / Q000209:etiology*; D005106:Exploratory Behavior / Q000502:physiology; D007839:Functional Laterality; D006624:Hippocampus / Q000473:pathology* / Q000503:physiopathology; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D020521:Stroke / Q000150:complications / Q000473:pathology*; D013997:Time Factors; D014741:Video Recording / Q000379:methods
chemical_list: D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; C092069:activity regulated cytoskeletal-associated protein; D001973:Bromodeoxyuridine