Altered CD19/CD22 balance in Egyptian children and adolescents with systemic lupus erythematosus.
B cells from systemic lupus erythematosus (SLE) patients display signalling defects that may underlie disease pathogenesis activity.CD19 and CD22 play a major role as regulators of B-cell response. The aim of this study was to clarify the relationship between B cell surface markers namely CD19, CD20 and CD22 expression and clinical and laboratory indices of SLE activity. The study included 33 SLE patients and 20 healthy children and adolescents as controls. Flowcytometric assay of dual markers, CD19/CD20, and CD20/CD22 was done. SLE disease activity was assessed by SLEDAI score. CD22% was significantly higher while CD20% was significantly lower in the study compared to the control group. No significant difference was observed in both groups with respect to CD19% or CD19/CD22% ratio. The level of CD22 expression was significantly lower in high and very high active cases than in mild and moderate cases and negatively correlated with SLDEAI score and ESR. Results obtained showed that, B cell surface receptors CD20 and CD22 are significantly affected in patients with SLE, pointing to their possible involvement in the aetiopathogenesis of the disease and in the regulatory mechanisms in response to the immune disturbance.
El-Sayed
Zeinab A
ZA
Department of Pediatrics, Faculty of Medicine, Ain Shams University, Egypt.
Ragab
Seham M
SM
Khalifa
Khaled A
KA
El Ashmawy
Ramy A
RA
D000293:Adolescent; D018941:Antigens, CD19 / Q000097:blood / Q000276:immunology*; D018951:Antigens, CD20 / Q000097:blood / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004534:Egypt; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007223:Infant; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008297:Male; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000097:blood / Q000276:immunology*
D018941:Antigens, CD19; D018951:Antigens, CD20; C497867:CD22 protein, human; D051918:Sialic Acid Binding Ig-like Lectin 2