Selective extraction of low molecular weight proteins by mesoporous silica particles with modified internal and external surfaces.
A new mesoporous silica material with modified external and internal surfaces (alkyl diol-vinyl-SiO(2)) was prepared and applied to selectively extract low molecular weight (LMW) proteins and peptides (less than 11 kDa) from biological samples. X-ray diffraction, N(2) adsorption, transmission electron microscopy, and Fourier transform infrared spectroscopy were used to characterize the alkyl diol-vinyl-SiO(2). This material was used to extract LMW proteins (peptides) from standard proteins, a mixture of peptides, and crude human plasma. We compared the amounts of different proteins adsorbed by alkyl diol-vinyl-SiO(2) and by vinyl-SiO(2) (vinyl group on the internal surface of the mesoporous silica material). The desorption recovery of alkyl diol-vinyl-SiO(2) by different eluents was also investigated by using a standard protein (insulin) as a model LMW protein. The material could efficiently extract LMW proteins and peptides from a mixture of standard proteins and crude human plasma with good extraction efficiency and desorption recovery. Furthermore, the alkyl diol-vinyl-SiO(2) had much better extraction selectivity for LMW proteins and peptides than a commercial C(18) solid phase extraction material.
Qi
Yanxia
Y
Department of Instrumentation and Analytical Chemistry, Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Wu
Dapeng
D
Wei
Junying
J
Ding
Kun
K
Wang
Hua
H
Zhang
Yangjun
Y
Qian
Xiaohong
X
Guan
Yafeng
Y
D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000097:blood / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*
D001798:Blood Proteins; D010455:Peptides; D011506:Proteins; D012822:Silicon Dioxide