Acetate accumulation through alternative metabolic pathways in ackA (-) pta (-) poxB (-) triple mutant in E. coli B (BL21).
Individual deletions of acs and aceA genes in E. coli B (BL21) showed little difference in the metabolite accumulation patterns but deletion of the ackA gene alone or together with pta showed acetic acid gradually accumulated to 3.1 and 1.7 g/l, respectively, with a minimal extended lag in bacterial growth and a higher pyruvate formation. Single poxB deletion in E. coli B (BL21) or additional poxB deletion in the ackA-pta mutants did not change the acetate accumulation pattern. When the acetate production genes (ackA-pta-poxB) were deleted in E. coli B (BL21) acetate still accumulated. This may be an indication that perhaps acetate is not only a by-product of carbon metabolism; it is possible that acetate plays also a role in other cellular metabolite pathways. It is likely that there are alternative acetate production pathways.
Phue
Je-Nie
JN
Biotechnology Core Laboratory, NIDDK NIH Bethesda, Bldg 14A Room 173, Bethesda, MD 20892, USA.
Lee
Sang Jun
SJ
Kaufman
Jeanne B
JB
Negrete
Alejandro
A
Shiloach
Joseph
J
D000084:Acetate Kinase / Q000235:genetics / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010709:Phosphate Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D011771:Pyruvate Oxidase / Q000235:genetics / Q000378:metabolism*
D000085:Acetates; D011771:Pyruvate Oxidase; D010709:Phosphate Acetyltransferase; D000084:Acetate Kinase