Effects of water cadmium concentrations on bioaccumulation and various oxidative stress parameters in Rhamdia quelen.
The effects of sublethal cadmium concentrations on oxidative stress parameters were evaluated in Rhamdia quelen. The fish were exposed to 0.44, 236, and 414 μg l⁻¹ cadmium for 7 and 14 days, followed by the same time periods for recovery. Enzymes, such as catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST), and indicators of oxidative stress, such as thiobarbituric acid-reactive species (TBARS) and protein carbonyl, were verified in fish tissues. In addition, the accumulation of cadmium was evaluated in these tissues. Our results indicate that CAT and GST levels decreased in gills after exposure periods associated with increased TBARS levels. In hepatic tissue, CAT, GST, TBARS, and protein carbonyl levels increased after 7 days of exposure, whereas SOD activity decreased after exposure for 14 days. In the kidney, TBARS levels decreased after exposure for 7 days and increased after exposure for 14 days. During the recovery periods, some variations persisted in gills, liver, and kidney. Cadmium accumulation was most significant in liver, followed by kidney and gills. These results indicate that cadmium concentrations studied invoke a stress response in silver catfish.
Pretto
Alexandra
A
Department of Chemistry, University of Santa Maria, Rio Grande do Sul, Brazil.
Loro
Vania Lucia
VL
Baldisserotto
Bernardo
B
Pavanato
Maria Amália
MA
Moraes
Bibiana Silveira
BS
Menezes
Charlene
C
Cattaneo
Roberta
R
Clasen
Bárbara
B
Finamor
Isabela Andres
IA
Dressler
Valderi
V
D000818:Animals; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity*; D002374:Catalase / Q000187:drug effects; D002397:Catfishes / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity*
D029941:Fish Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D002104:Cadmium; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase