The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence.
cis-jasmone (CJ) is a plant-derived chemical that enhances direct and indirect plant defence against herbivorous insects. To study the signalling pathway behind this defence response, we performed microarray-based transcriptome analysis of CJ-treated Arabidopsis plants. CJ influenced a different set of genes from the structurally related oxylipin methyl jasmonate (MeJA), suggesting that CJ triggers a distinct signalling pathway. CJ is postulated to be biosynthetically derived from jasmonic acid, which can boost its own production through transcriptional up-regulation of the octadecanoid biosynthesis genes LOX2, AOS and OPR3. However, no effect on these genes was detected by treatment with CJ. Furthermore, CJ-responsive genes were not affected by mutations in COI1 or JAR1, which are critical signalling components in MeJA response pathway. Conversely, a significant proportion of CJ-inducible genes required the three transcription factors TGA2, TGA5 and TGA6, as well as the GRAS regulatory protein SCARECROW-like 14 (SCL14), indicating regulation by a different pathway from the classical MeJA response. Moreover, the biological importance was demonstrated in that mutations in TGA2, 5, 6, SCL14 and the CJ-inducible gene CYP81D11 blocked CJ-induced attraction of the aphid parasitoid Aphidius ervi, demonstrating that these components play a key role in CJ-induced indirect defence. Collectively, our results identify CJ as a member of the jasmonates that controls indirect plant defence through a distinct signalling pathway.
Matthes
Michaela C
MC
Biological Chemistry Department, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Bruce
Toby J A
TJ
Ton
Jurriaan
J
Verrier
Paul J
PJ
Pickett
John A
JA
Napier
Johnathan A
JA
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D015152:Blotting, Northern; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D009713:Nucleotidyltransferases / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D010453:Peptide Synthases
D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D003517:Cyclopentanes; D009687:Nuclear Proteins; D009713:Nucleotidyltransferases; D054883:Oxylipins; C489413:AHBP-1b protein, Arabidopsis; C112279:COI1 protein, Arabidopsis; C084751:OBF5 protein, Arabidopsis; C107090:TGA6 protein, Arabidopsis; C086299:jasmone; C412831:AT2G46370 protein, Arabidopsis; D010453:Peptide Synthases