[Regulatory effect of complex of natural cytokines and antimicrobial peptides on murine macrophages infected with multidrug-resistant Mycobacterium tuberculosis].
OBJECTIVE
To study the effect of complex of natural cytokines and antimicrobial peptides (CNCAP) included in preparation Superlymph on growth of multidrug-resistant Mycobacterium tuberculosis CN-37 on the modem of murine peritoneal macrophages (MPh) cultivated in vitro.


METHODS
Effect of CNCAP on peritoneal MPh of tuberculosis-susceptible mice C57BL/6 infected by M. tuberculosis CN-37 was studied using ex vivo model. Macrophages were preliminary incubated with CNCAP during one day. M. tuberculosis growth was assessed on 7th day by PCR.


RESULTS
Preliminary incubation of infected MPh with CNCAP resulted in inhibition of M. tuberculosis CN-37 growth.


CONCLUSIONS
Superlymph activates macrophages which lead to enhanced bactericidal action of MPh on M. tuberculosis CN-37.
Koval'chuk
L V
LV
Gvozdeva
Iu V
IuV
Chernousova
L N
LN
Gankovskaia
L V
LV
Smirnova
T G
TG
Larionova
E E
EE
Sokolova
E V
EV
Levchenko
V A
VA
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D000995:Antitubercular Agents; D016207:Cytokines