Hydrogen sulfide stimulates β-amylase activity during early stages of wheat grain germination.
We recently reported that H 2S could significantly promote the germination of wheat grains subjected to aluminum (Al(3+)) stress.1 In these experiments seeds were pretreated with the H 2S donor NaHS for 12 h prior to Al(3+) stress. During this pre-incubation period we observed that H2S increased the activity of grain amylase in the absence of Al(3+). Using embryoless half grains of wheat we now show that H2S preferentially affects the activity of endosperm β-amylase and that α-amylase synthesis and activity is unaffected by this treatment.
Zhang
Hua
H
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui, China. zhandzhy@yahoo.com.cn
Dou
Wei
W
Jiang
Cheng-Xi
CX
Wei
Zhao-Jun
ZJ
Liu
Jian
J
Jones
Russell L
RL
D056625:Endosperm / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D018525:Germination; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D001614:beta-Amylase / Q000378:metabolism*
D010940:Plant Proteins; D001614:beta-Amylase; D006862:Hydrogen Sulfide