(15)N-Labeled ionic probes for bioanalytical mass spectrometry.
An effective La-complex-based probe ionization method is reported. Novel stable isotopically labeled probes containing the (15)N-labeled 2,6-bis(oxazolin-2-yl)pyridine (pybox) ligand, succinimide-tetramethylpybox (NHS-TMpybox), maleimide-tetramethylpybox (Mal-TMpybox), and 4-(tetramethylpybox)-butyl bromoacetate (BrAc-TMpybox), have been synthesized and their value in analyzing large complex molecules has been studied. The value of the (15)N-labeled pybox-La complex in ionizing various compounds, including bioactive peptides by cold-spray ionization mass spectrometry is emphasized.
Ito
Fumihiro
F
Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. h-itoh@kph.bunri-u.ac.jp
Ando
Shin
S
Iuchi
Masato
M
Nakamura
Tomoko
T
Satoko Yorita
Yamaguchi
Kentaro
K
D000085:Acetates / Q000138:chemical synthesis / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D007477:Ions / Q000138:chemical synthesis / Q000737:chemistry; D007811:Lanthanum / Q000737:chemistry; D008024:Ligands; D008301:Maleimides / Q000138:chemical synthesis / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D009587:Nitrogen Isotopes / Q000138:chemical synthesis / Q000737:chemistry; D010080:Oxazoles / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry*; D013388:Succinimides / Q000138:chemical synthesis / Q000737:chemistry
D000085:Acetates; D002264:Carboxylic Acids; D007477:Ions; D008024:Ligands; D008301:Maleimides; D009587:Nitrogen Isotopes; D010080:Oxazoles; D010455:Peptides; D011725:Pyridines; D013388:Succinimides; C043592:maleimide; C016274:bromoacetate; D007811:Lanthanum