High-content analysis of single cells directly assembled on CMOS sensor based on color imaging.
A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66 mm×5.32 mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
Tanaka
Tsuyoshi
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, 184-8588 Tokyo, Japan.
Saeki
Tatsuya
T
Sunaga
Yoshihiko
Y
Matsunaga
Tadashi
T
D000906:Antibodies; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003116:Color; D004396:Coloring Agents; D006367:HeLa Cells; D006735:Horseradish Peroxidase; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D019044:Image Cytometry / Q000295:instrumentation* / Q000379:methods; D049449:Luminescence; D012666:Semiconductors; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods; D014343:Trypan Blue
D000906:Antibodies; C497483:CD44 protein, human; D004396:Coloring Agents; D018960:Hyaluronan Receptors; D006735:Horseradish Peroxidase; D014343:Trypan Blue