title: Oligopeptide delivery carrier for osteoclast precursors.
abstract: Dendritic amine and guanidinium group-modified nanoparticles were investigated for the delivery of model peptide drug into primary osteoclast precursor cells (bone marrow macrophages; BMMs). The model peptide drug was encapsulated into the nanoparticle by dropping the drug/carrier dissolved in dimethylsulfoxide/methylene chloride cosolvent into water containing poly(vinyl alcohol) as a stabilizer. Flow cytometry and spectrofluorimetry analysis indicated that the model drug itself was not taken up by the BMMs; however, nanoparticle systems underwent significant cellular uptake. In particular, guanidinium group-modified nanoparticles were taken up more efficiently than amine group-modified ones. Cell viability studies showed that both amine and guanidinium group-modified nanoparticles exhibited no significant cytotoxicity up to 100 microg/mL against the cells.
authors: Department of Chemistry and Nano Science and Division of Life and Pharmaceutical Sciences, Department of Bioinspired Science, Ewha Womans University, Seoul, Korea.
authors: Bo
authors: B
authors: Chi
authors: So Jeong
authors: SJ
authors: Park
authors: Min Hee
authors: MH
authors: Park
authors: Soo Young
authors: SY
authors: Lee
authors: Byeongmoon
authors: B
authors: Jeong
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005260:Female; D019791:Guanidine / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D010010:Osteoclasts / Q000166:cytology*; D010316:Particle Size
chemical_list: D000588:Amines; D004337:Drug Carriers; D009842:Oligopeptides; D019791:Guanidine