Effects of lindane on the accumulation of cyclic AMP, induced by vasoactive intestinal peptide, in isolated rat prostatic epithelial cells.
Lindane pretreatment of isolated rat prostatic epithelial cells resulted in a time- and dose-dependent impairment of the stimulation of cyclic AMP levels by vasoactive intestinal peptide (VIP); the optimal conditions for producing this impairment were found to be 5 min of cell exposure to 0.2 mm-lindane at 25 degrees C. The inhibitory effect of the insecticide was related to a decrease in VIP efficiency since the maximal cyclic AMP response (at 100 nm VIP) was about 50% of that in control cells. VIP potency was unaffected since the half-maximal cyclic AMP response was elicited at about 3 nm VIP in both the control and lindane-pretreated cells. The results indicate that lindane interferes with the cyclic AMP system in prostatic epithelium, and this could be a consequence of some interaction in the prostate gland between the two main systems for signal transduction (i.e. adenylate cyclase and phosphatidylinositol/Ca(2+)/protein kinase C).
Carrero
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Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
Pérez-Albarsanz
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Recio
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Prieto
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