[Separation of gamma linolenic acid from evening primrose oil with urea inclusion--orthogonal experiment of optimizing technological parameters and observation of urea inclusion compound I].
OBJECTIVE
The influence on the urea inclusion compound under different conditions (allocated proportion, time of inclusion, temperature of inclusion) were studied through the orthogonal test, and theoretical reference of urea inclusion process for further optimization wound be offered.


METHODS
The orthogonal experiment was adopted, and microscope was used to observe the shape, aperture size of the urea inclusion compound under different technological parameters, the GC was employed to inspect the purity of GLA.


RESULTS
The results indicated that the ratio of fatty acids and urea, inclusion of temperature, time of inclusion had great effect on urea inclusion compound. The three factors and its interactions significantly affected the purity of GLA. The results also showed that the best process was that the ratio of fatty acids and urea was 1 : 3, temperature of inclusion was--15 degrees C, time of inclusion was 24 h.


CONCLUSIONS
Under the best condition, the purity of GLA reach up to 95.575 9%; and it is feasible to observe the shape and the amount of the urea inclusion compound to reflect and guide the urea inclusion technology.
Wang
Hua
H
College of Food Science and Engineering, College of Enology, Northwest Agriculture and Forestry University, Yangling 712100, China. wanghua@nwsuaf.edu.cn
Ling
Man
M
Xue
Gang
G
Liu
Fengxia
F
Guo
Shuxian
S
D031665:Oenothera biennis / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D014508:Urea / Q000737:chemistry*; D017965:gamma-Linolenic Acid / Q000032:analysis / Q000302:isolation & purification*
D010938:Plant Oils; D017965:gamma-Linolenic Acid; D014508:Urea