Selective fluorescence sensing of deoxycytidine 5'-monophosphate (dCMP) employing a bis(diphenylphosphate)diimine ligand.
A new bis(diphenylphosphate)diimine ligand (BP1) was prepared and evaluated for its ability for selective detection of deoxycytidine 5'-monophosphate (dCMP). BP1 exhibited off-type fluorescence in the presence of dCMP. The fluorescence of BP1 was significantly quenched upon the addition of 2.5 × 10(-4) M dCMP and the detection limit was 1.25 × 10(-5) M in MeCN-H(2)O (1:1, v/v). The binding ratio between BP1 and dCMP was determined to be 1:1 with the binding constant of 3.98 ± 0.60 × 10(-3) M(-1).
Nasomphan
Weerachai
W
Bioresources Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC) Thailand Science Park, Klong Luang, Pathumthani, Thailand.
Tangboriboonrat
Pramuan
P
Smanmoo
Srung
S
D001713:Biphenyl Compounds / Q000737:chemistry*; D003843:Deoxycytidine Monophosphate / Q000032:analysis*; D007097:Imines / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
D001713:Biphenyl Compounds; D007097:Imines; D008024:Ligands; C557990:bis(diphenylphosphate)diimine; D003843:Deoxycytidine Monophosphate