title: Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.
abstract: The purpose of the present study was to investigate the effect of acute exercise on lipolysis via coordination of hormone-sensitive lipase (HSL) and scaffold proteins, i.e., perilipin A and comparative gene identification-58 (CGI-58), in rat primary adipocytes. Glycerol release was significantly elevated immediately (0h) and three hours (3h) after exercise. Both activity and localization to the pellet of HSL were significantly greater in the pellet fraction, which is included in lipid droplet associated-proteins, than in the supernatant fraction. In the pellet fraction, although neither perilipin A nor CGI-58 protein level changed, level of perilipin A/CGI-58 complex was significantly reduced, accompanied by up-regulated association of perilipin A/HSL at 0h and 3h after exercise. On the other hand, there were no changes in these molecules at 24h after exercise, despite a significant decrease in lipolysis that was observed in response to isoproterenol. These findings suggest that acute exercise enhances lipolysis up to at least 3h after exercise in a manner dependent on modification of HSL and its association with and alteration in scaffold protein.
authors: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan. junetsu@ks.kyorin-u.ac.jp
authors: Junetsu
authors: J
authors: Ogasawara
authors: Sachiko
authors: S
authors: Nomura
authors: Nazibur
authors: N
authors: Rahman
authors: Takuya
authors: T
authors: Sakurai
authors: Takako
authors: T
authors: Kizaki
authors: Tetsuya
authors: T
authors: Izawa
authors: Hitoshi
authors: H
authors: Ishida
authors: Shukoh
authors: S
authors: Haga
authors: Hideki
authors: H
authors: Ohno
mesh_terms: D000217:Acyltransferases; D017667:Adipocytes / Q000201:enzymology*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D008066:Lipolysis; D008297:Male; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000096:biosynthesis; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: C520594:Abhd5 protein, rat; D002352:Carrier Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins; D000217:Acyltransferases; D002787:Sterol Esterase