Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells.
OBJECTIVE
the purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates.


METHODS
we used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism.


RESULTS
CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos.


CONCLUSIONS
these potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
Lee
Maw-Sheng
MS
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Liu
Chung-Hsien
CH
Lee
Tsung-Hsien
TH
Wu
Hui-Mei
HM
Huang
Chun-Chia
CC
Huang
Lii-Shung
LS
Chen
Chuan-Mu
CM
Cheng
En-Hui
EH
D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000378:metabolism; D052276:Creatine Kinase, BB Form / Q000235:genetics / Q000378:metabolism*; D054885:Cumulus Cells / Q000166:cytology / Q000201:enzymology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D015415:Biomarkers; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; D052276:Creatine Kinase, BB Form