title: MicroRNA-target pairs in human renal epithelial cells treated with transforming growth factor beta 1: a novel role of miR-382.
abstract: We reported previously an approach for identifying microRNA (miRNA)-target pairs by combining miRNA and proteomic analyses. The approach was applied in the present study to examine human renal epithelial cells treated with transforming growth factor β1 (TGFβ1), a model of epithelial-mesenchymal transition important for the development of renal interstitial fibrosis. Treatment of human renal epithelial cells with TGFβ1 resulted in upregulation of 16 miRNAs and 18 proteins and downregulation of 17 miRNAs and 16 proteins. Of the miRNAs and proteins that exhibited reciprocal changes in expression, 77 pairs met the sequence criteria for miRNA-target interactions. Knockdown of miR-382, which was up-regulated by TGFβ1, attenuated TGFβ1-induced loss of the epithelial marker E-cadherin. miR-382 was confirmed by 3'-untranslated region reporter assay to target five genes that were downregulated at the protein level by TGFβ1, including superoxide dismutase 2 (SOD2). Knockdown of miR-382 attenuated TGFβ1-induced downregulation of SOD2. Overexpression of SOD2 ameliorated TGFβ1-induced loss of the epithelial marker. The study provided experimental evidence in the form of reciprocal expression at the protein level for a large number of predicted miRNA-target pairs and discovered a novel role of miR-382 and SOD2 in the loss of epithelial characteristics induced by TGFβ1.
authors: Department of Physiology, Biotechnology and Biomedical Engineering Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors: Alison J
authors: AJ
authors: Kriegel
authors: Yi
authors: Y
authors: Fang
authors: Yong
authors: Y
authors: Liu
authors: Zhongmin
authors: Z
authors: Tian
authors: Domagoj
authors: D
authors: Mladinov
authors: Isaac R
authors: IR
authors: Matus
authors: Xiaoqiang
authors: X
authors: Ding
authors: Andrew S
authors: AS
authors: Greene
authors: Mingyu
authors: M
authors: Liang
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney / Q000166:cytology*; D035683:MicroRNAs / Q000378:metabolism* / Q000502:physiology; D020543:Proteome / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D000099156:Superoxide Dismutase 2
chemical_list: D035683:MicroRNAs; D020543:Proteome; D013482:Superoxide Dismutase; D053773:Transforming Growth Factor beta1; D000099156:Superoxide Dismutase 2; C555413:MIRN382 microRNA, human