Production and characterization of a biodegradable poly (hydroxybutyrate-co-hydroxyvalerate) (PHB-co-PHV) copolymer by moderately haloalkalitolerant Halomonas campisalis MCM B-1027 isolated from Lonar Lake, India.
Several microorganisms produce polyhydroxyalkanoates (PHA). They are accumulated intracellularly as energy storage compounds. The PHAs are of interest because of their potential in biomedical applications. Halophilic bacteria and archaea are known to produce polyhydroxybutyrate (PHB). This paper describes production of a biodegradable copolymer, PHB-co-PHV by a moderately haloalkalitolerant Halomonas campisalis, isolated from Lonar Lake, India. The production of PHA was in the range of 45-81% on dry cell weight basis when the organism was grown in a production medium containing 1% (w/v) maltose and 0.1% (w/v) yeast extract, at pH ranging from 6 to 9 with an inoculum density of 10(5)-10(7) cells/ml of medium, for incubation period of 15-30 h and at 37 degrees C. The polymer produced by the organism is a hydroxyester with molecular weight of 1.3014 x 10(6). Its melting temperature was 171 degrees C. The (1)H NMR analysis revealed that the polymer was a copolymer of PHB-co-PHV. This could be achieved by providing simple carbon source viz. maltose.
Kulkarni
S O
SO
Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune 411 004, India.
Kanekar
P P
PP
Nilegaonkar
S S
SS
Sarnaik
S S
SS
Jog
J P
JP
D000222:Adaptation, Physiological / Q000187:drug effects*; D000468:Alkalies / Q000494:pharmacology*; D000704:Analysis of Variance; D001673:Biodegradation, Environmental / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D003470:Culture Media / Q000494:pharmacology; D005618:Fresh Water / Q000382:microbiology*; D020632:Halomonas / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007194:India; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011091:Polyesters / Q000378:metabolism*; D012015:Reference Standards; D012492:Salts / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D000468:Alkalies; D003470:Culture Media; D011091:Polyesters; D012492:Salts; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates