Mugwort-sensitized individuals from North Europe, South Europe and North America show different IgE reactivity patterns.
BACKGROUND
Sensitization to weed pollen allergens at the molecular level is not fully understood. We studied IgE reactivity to the major mugwort allergen Art v 1 in relation to allergens from other weed pollen and cross-reactive components in mugwort-sensitized subjects.


METHODS
Art v 1 and Amb a 1 were affinity purified and coupled to experimental ImmunoCAP® tests. Samples from North Europe (n = 50), South Europe (n = 19) and North America (n = 41) were analyzed for IgE against mugwort pollen, weed allergen components, pan-allergens and cross-reactive carbohydrate determinants (CCDs).


RESULTS
The prevalence of IgE reactivity (>0.35 kU(A)/l) to Art v 1 was significantly higher in samples from North Europe than in those from North America. IgE to Amb a 1 was more common in North America than in North and South Europe, while IgE to Par j 2 was common in South Europe, less common in North America, and absent in North Europe. IgE to Art v 3 in mugwort-allergic patients was more common in North Europe than in South Europe and North America, while IgE to Sal k 1 was similar between the areas. Subjects with an Art v 1/mugwort-specific IgE ratio <0.5 had more often IgE to Amb a 1, profilin, polcalcin and CCDs than subjects with a ratio >0.5.


CONCLUSIONS
Mugwort-sensitized subjects have different IgE reactivity profiles to weed allergens, reflecting their exposure to various pollens. Subjects with a low ratio between the IgE levels to Art v 1 and mugwort have a diverse IgE reactivity profile, indicating a role for cross-reactive allergens in their mugwort sensitization.
Movérare
Robert
R
Phadia AB, R&D, Uppsala University, Uppsala, Sweden. robert.moverare@phadia.com
Larsson
Håkan
H
Carlsson
Raimo
R
Holmquist
Ingrid
I
D000818:Animals; D000911:Antibodies, Monoclonal; D019306:Artemisia / Q000276:immunology*; D005060:Europe / Q000453:epidemiology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009656:North America / Q000453:epidemiology; D015995:Prevalence; D006255:Rhinitis, Allergic, Seasonal / Q000453:epidemiology / Q000276:immunology*; D020539:Sequence Analysis, Protein; D012882:Skin Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric
D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E