title: Expression of NF-E2-related factor 2 (Nrf2) in the basilar artery after experimental subarachnoid hemorrhage in rabbits: a preliminary study.
abstract: It has been suggested that the pathogenesis of vasospasm is complex including endothelial damage, oxidative stress, inflammatory damage, and the accumulation of toxic metabolites. Recently, a growing body of evidence indicates that nuclear factor erythroid 2-related factor 2 (Nrf2) plays a unique role in many physiological stress processes. In this study, a total of 48 rabbits were assigned randomly to four groups: control group, SAH day 3, day 5, and day 7 groups. The animals in SAH day 3, day 5, and day 7 groups were subjected to injection of autologous blood into cisterna magna twice on day 0 and day 2 and were killed on days 3, 5, and 7, respectively. Cross-sectional area of basilar artery was measured and the Nrf2 expression was assessed by immunohistochemistry and Western blot analysis. The mRNA levels of Nrf2 were also determined by RT-PCR. The basilar arteries exhibited vasospasm after SAH and became more severe on days 3 and 5. The elevated expression of Nrf2 was detected after SAH and peaked on days 3 and 5. Nrf2 is increasingly expressed in a parallel time course to the development of cerebral vasospasm in a rabbit experimental model of SAH.
authors: Department of Neurosurgery, Wuxi Second Hospital Affiliated Nanjing Medical University, 68 Zhong Shan Road, Wuxi 214002, Jiangsu Province, China.
authors: Xu-Dong
authors: XD
authors: Zhao
authors: Yi-Ting
authors: YT
authors: Zhou
authors: Xing
authors: X
authors: Zhang
authors: Xiao-Liang
authors: XL
authors: Wang
authors: Wu
authors: W
authors: Qi
authors: Zong
authors: Z
authors: Zhuang
authors: Xing-Fen
authors: XF
authors: Su
authors: Ji-Xin
authors: JX
authors: Shi
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D009130:Muscle, Smooth / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D013345:Subarachnoid Hemorrhage / Q000473:pathology*; D013997:Time Factors
chemical_list: D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger