Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide.
OBJECTIVE
To evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo.


METHODS
Aliquots of 1 × 10(4) murine fibroblasts were incubated at 37 °C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 μg mL(-1) individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test.


RESULTS
Mineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement.


CONCLUSIONS
White MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.
Zeferino
E G
EG
Endodontic Area, Center for Dental Research, São Leopoldo Mandic University, Campinas, Brazil.
Bueno
C E S
CE
Oyama
L M
LM
Ribeiro
D A
DA
D041721:3T3-L1 Cells / Q000187:drug effects*; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001729:Bismuth / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity*; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D020552:Comet Assay; D004249:DNA Damage; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D008422:Materials Testing; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D010087:Oxides / Q000633:toxicity*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013696:Temperature; D013997:Time Factors; D014343:Trypan Blue
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D009153:Mutagens; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C033301:bismuth oxide; D014343:Trypan Blue; D001729:Bismuth