title: Biomolecular-motor-based autonomous delivery of lipid vesicles as nano- or microscale reactors on a chip.
abstract: We aimed to create an autonomous on-chip system that performs targeted delivery of lipid vesicles (liposomes) as nano- or microscale reactors using machinery from biological systems. Reactor-liposomes would be ideal model cargoes to realize biomolecular-motor-based biochemical analysis chips; however, there are no existing systems that enable targeted delivery of cargo-liposomes in an autonomous manner. By exploiting biomolecular-motor-based motility and DNA hybridization, we demonstrate that single-stranded DNA (ssDNA)-labeled microtubules (MTs), gliding on kinesin-coated surfaces, acted as cargo transporters and that ssDNA-labeled cargo-liposomes were loaded/unloaded onto/from gliding MTs without bursting at loading reservoirs/micropatterned unloading sites specified by DNA base sequences. Our results contribute to the development of an alternative strategy to pressure-driven or electrokinetic flow-based microfluidic devices.
authors: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. hiyama@nttdocomo.co.jp
authors: Satoshi
authors: S
authors: Hiyama
authors: Yuki
authors: Y
authors: Moritani
authors: Riho
authors: R
authors: Gojo
authors: Shoji
authors: S
authors: Takeuchi
authors: Kazuo
authors: K
authors: Sutoh
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D008081:Liposomes / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D020409:Molecular Motor Proteins / Q000737:chemistry*; D009038:Motion; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size
chemical_list: D008081:Liposomes; D020409:Molecular Motor Proteins