title: Effect of molecular mass of methoxypoly(ethylene glycol) activated with succinimidyl carbonate on camouflaging pancreatic islets.
abstract: The surface modification of Langerhans islets by grafting activated poly(ethylene glycol) on to their capsules in order to prevent immune-system stimulation is a novel method in diabetes cell therapy. In the present study, mPEG [methoxypoly(ethylene glycol)] with two molecular masses of 5 and 10 kDa, activated with SC (succinimidyl carbonate), was grafted on to the surface of pancreatic islets at a polymer concentration of 22 mg/ml. It was found that PEGylated islets were viable and active, and no morphological changes on the collagen capsule of islets were observed. The amount of interleukin-2 secretion from lymphocytes co-cultured with islets PEGylated with mPEG-SC of 5 and 10 kDa was 112.12 ± 23.09 pg/ml and 172.75 ± 27.94 pg/ml respectively. Thus mPEG-SC (SC-activated mPEG) with higher molecular mass was more suitable for camouflaging islets from the immune system.
authors: Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, 1411713116, Iran.
authors: Leila
authors: L
authors: Barani
authors: Ebrahim
authors: E
authors: Vasheghani-Farahani
authors: Hamideh Aghajani
authors: HA
authors: Lazarjani
authors: Sameereh
authors: S
authors: Hashemi-Najafabadi
authors: Fatemeh
authors: F
authors: Atyabi
mesh_terms: D000818:Animals; D002254:Carbonates / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D003920:Diabetes Mellitus / Q000601:surgery; D007376:Interleukin-2 / Q000378:metabolism; D007515:Islets of Langerhans / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013388:Succinimides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002254:Carbonates; D007376:Interleukin-2; D013388:Succinimides; C085786:succinimidyl carbonate; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol