In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves.
OBJECTIVE
To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models.


METHODS
Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 microg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p).


RESULTS
The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 microg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight.


CONCLUSIONS
The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.
Raj
Vasanth P
VP
Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India.
Chandrasekhar
Raghu H
RH
P
Vijayan
V
S A
Dhanaraj
D
Rao
Mallikarjuna C
MC
Rao
Venkata J
VJ
Nitesh
K
K