[Effect of polypeptide from Chlamys farreri on ultraviolet A-induced Fas and c-fos expression in HaCaT cells].
OBJECTIVE
To observe the effect of Polypeptide from Chlamys farreri (PCF) on expression levels of Fas and c-fos in HaCaT cells after ultraviolet A (UVA) radiation, and investigate the molecular mechanism of PCF protecting against UVA-induced damage in HaCaT cells.


METHODS
HaCaT cells were divided into five groups: control group, UVA model group, 5.69 mmol/L PCF group, 2.84 mmol/L PCF group, and 1.42 mmol/L PCF group. Expression levels of Fas and c-fos mRNA was assayed by PCR. Western blot analysis was used to determine the protein levels of Fas and c-fos.


RESULTS
After UVA exposure, Fas and c-fos expression increased significantly (P < 0.01). 1.42 - 5.69 mmol/L PCF inhibited UVA-induced mRNA and protein expressions of Fas and c-fos in HaCaT cells in dose-dependent manner (P < 0.05).


CONCLUSIONS
PCF could attenuate UVA-induced Fas and c-fos expression, which may attribute to its protective effect against UVA-induced damage in HaCaT cells.
Li
Jinlian
J
Binzhou Medical College, Binzhou 256603, China. ljl813@sina.com
Li
Na
N
Dong
Xiaoqing
X
Wang
Chunbo
C
D000818:Animals; D002460:Cell Line; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D049897:Pectinidae / Q000737:chemistry; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
C491901:FAS protein, human; D010455:Peptides; D016760:Proto-Oncogene Proteins c-fos; D019014:fas Receptor