Adipocyte fatty acid-binding protein: an important gene related to lipid metabolism in chicken adipocytes.
Fatty acid-binding proteins (FABPs) may facilitate the diffusion of fatty acids within the cytoplasm. Adipocyte FABP (A-FABP) null mice exhibit altered rates of lipolysis, and reprogrammed expression of genes related to lipid metabolism. In the current study, chicken adipocytes with short hairpin RNA-mediated knockdown and cDNA overexpression of A-FABP were used to investigate the role of chicken A-FABP in lipid metabolism of adipocytes. The results showed that there was no significant change of lipid metabolism in the A-FABP interference adipocytes. However, in the A-FABP overexpression adipocytes, the lipolysis was increased significantly at 36 h (p<0.05), and lipid accumulation was increased significantly at 48, 60 and 72 h (p<0.05). The expression of PPAR γ and perilipin genes were upregulated, while the expression of E-FABP gene was downregulated after transfection with pcDNA3.1/A-FABP (p<0.05). The present study suggested that A-FABP gene might affect the lipid metabolism through the PPAR γ pathway, and the function between A-FABP and E-FABP gene was compensatory in the chickens.
Shi
Hui
H
College of Animal Science and Technology, Northeast Agricultural University, Harbin, PR China.
Wang
Qigui
Q
Wang
Yuxiang
Y
Leng
Li
L
Zhang
Qingqiu
Q
Shang
Zhouchun
Z
Li
Hui
H
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics; D034622:RNA Interference
D030161:Avian Proteins; D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified