Immunopathology and cytokine responses in commercial broiler chickens with gangrenous dermatitis.
Gangrenous dermatitis (GD) is an emerging disease of increasing economic importance in poultry resulting from infection by Clostridium septicum and Clostridium perfringens type A. Lack of a reproducible disease model has been a major obstacle in understanding the immunopathology of GD. To gain better understanding of host-pathogen interactions in GD infection, we evaluated various immune parameters in two groups of birds from a recent commercial outbreak of GD, the first showing typical disease signs and pathological lesions (GD-like birds) and the second lacking clinical signs (GD-free birds). Our results revealed that GD-like birds showed: reduced T-cell and B-cell mitogen-stimulated lymphoproliferation; higher levels of serum nitric oxide and alpha-1-acid glycoprotein; greater numbers of K55(+), K1(+), CD8(+), and MHC class II(+) intradermal lymphocytes, and increased K55(+), K1(+), CD8(+), TCR1(+), TCR2(+), Bu1(+), and MHC class II(+) intestinal intraepithelial lymphocytes; and increased levels of mRNAs encoding proinflammatory cytokines and chemokines in skin compared with GD-free chickens. These results provide the first evidence of altered systemic and local (skin and intestine) immune responses in GD pathogenesis in chickens.
Li
Guangxing
G
Animal and Natural Resources Institute, USDA-ARS, Beltsville, MD, USA.
Lillehoj
Hyun S
HS
Lee
Kyung Woo
KW
Lee
Sung Hyen
SH
Park
Myeong Seon
MS
Jang
Seung I
SI
Bauchan
Gary R
GR
Gay
Cyril G
CG
Ritter
G Donald
GD
Bautista
Daniel A
DA
Siragusa
Gregory R
GR
D000818:Animals; D000911:Antibodies, Monoclonal; D049109:Cell Proliferation; D018925:Chemokines / Q000276:immunology; D002645:Chickens; D003013:Clostridium / Q000235:genetics / Q000472:pathogenicity*; D016207:Cytokines / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D003691:Delaware; D003872:Dermatitis / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005734:Gangrene / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000276:immunology*; D009569:Nitric Oxide / Q000097:blood; D009961:Orosomucoid / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D018925:Chemokines; D016207:Cytokines; D017931:DNA Primers; D009961:Orosomucoid; D009569:Nitric Oxide