title: Cellular localization of genes and proteins for tumor necrosis factor-α (TNF), TNF receptor types I and II in bovine endometrium.
abstract: To determine which cell types produce tumor necrosis factor-α (TNF) and its receptors (TNFRI and TNFRII) in bovine endometrium, we investigated the expression and cellular localization of their mRNAs and proteins. TNF transcripts and proteins were co-localized in endometrial epithelial cells, glandular epithelial cells and endothelial cells of microvessels but not in the stromal cells. TNF protein was detected in the lysate and the cultured media of epithelial cells, but was only weakly detected in the stromal cells. Both TNFRI (TNFRSF1A) and TNFRII (TNFRSF1B) transcripts were expressed in the epithelial cells, glandular epithelial cells and the stromal cells, whereas their proteins were weakly expressed in the stroma. TNF mRNA and protein expressions in the cultured epithelial cells were increased by TNF and interleukin-1α, and the TNFRII mRNA expressions were stimulated by oxytocin. Together, TNF secreted by the endometrial cells may locally play a role in regulating uterine function throughout the estrous cycle.
authors: Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. kokuda@cc.okayama-u.ac.jp
authors: K
authors: K
authors: Okuda
authors: R
authors: R
authors: Sakumoto
authors: N
authors: N
authors: Okamoto
authors: T J
authors: TJ
authors: Acosta
authors: H
authors: H
authors: Abe
authors: H
authors: H
authors: Okada
authors: F
authors: F
authors: Sinowatz
authors: D J
authors: DJ
authors: Skarzynski
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology; D053582:Interleukin-1alpha / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics* / Q000378:metabolism; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D053582:Interleukin-1alpha; D012333:RNA, Messenger; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; D010121:Oxytocin; D006854:Hydrocortisone