The moss Physcomitrella patens contains cyclopentenones but no jasmonates: mutations in allene oxide cyclase lead to reduced fertility and altered sporophyte morphology.
• Two cDNAs encoding allene oxide cyclases (PpAOC1, PpAOC2), key enzymes in the formation of jasmonic acid (JA) and its precursor (9S,13S)-12-oxo-phytodienoic acid (cis-(+)-OPDA), were isolated from the moss Physcomitrella patens. • Recombinant PpAOC1 and PpAOC2 show substrate specificity against the allene oxide derived from 13-hydroperoxy linolenic acid (13-HPOTE); PpAOC2 also shows substrate specificity against the allene oxide derived from 12-hydroperoxy arachidonic acid (12-HPETE). • In protonema and gametophores the occurrence of cis-(+)-OPDA, but neither JA nor the isoleucine conjugate of JA nor that of cis-(+)-OPDA was detected. • Targeted knockout mutants for PpAOC1 and for PpAOC2 were generated, while double mutants could not be obtained. The ΔPpAOC1 and ΔPpAOC2 mutants showed reduced fertility, aberrant sporophyte morphology and interrupted sporogenesis.
Stumpe
Michael
M
Georg-August-University, Albrecht-von-Haller-Institute, Plant Biochemistry, Göttingen, Germany.
Göbel
Cornelia
C
Faltin
Bernd
B
Beike
Anna K
AK
Hause
Bettina
B
Himmelsbach
Kiyoshi
K
Bode
Julia
J
Kramell
Robert
R
Wasternack
Claus
C
Frank
Wolfgang
W
Reski
Ralf
R
Feussner
Ivo
I
D016718:Arachidonic Acid / Q000378:metabolism; D019068:Bryopsida / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000302:isolation & purification; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism*; D051479:Plant Infertility / Q000235:genetics*; D011994:Recombinant Proteins; D013379:Substrate Specificity; D017962:alpha-Linolenic Acid / Q000378:metabolism
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D011994:Recombinant Proteins; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; C011006:jasmonic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase; C013905:cyclopentenone