Transmitted HIV drug resistance among HIV-infected voluntary counseling and testing centers (VCTC) clients in Mumbai, India.
A survey for transmitted HIV drug resistance (HIVDR) was conducted according to WHO guidelines among clients newly diagnosed with HIV-1 infection at two voluntary counseling and testing centers (VCTC) in Mumbai. HIVDR testing was performed using the ViroSeq RT-PCR method (Abbott). Out of 50 successfully amplified and sequenced specimens, analysis of the first 34 consecutively collected specimens revealed no nucleoside reverse transcriptase inhibitor, nonnucleoside reverse transcriptase inhibitor, or protease inhibitor mutations from the 2007 WHO list of mutations for surveillance of transmitted HIVDR, indicating that the prevalence of transmitted HIVDR to all three drug classes was <5% among recently infected VCTC clients in Mumbai. The phylogenetic analysis revealed that all samples belonged to HIV-1 subtype C. Continued ART program monitoring and further evaluation of transmitted HIV drug resistance in coming years are essential in Mumbai as well as in other regions of the country in which ART is being scaled up rapidly.
Chaturbhuj
Devidas N
DN
National AIDS Research Institute, Bhosari, Pune, India.
Hingankar
Nitin K
NK
Srikantiah
Padmini
P
Garg
Renu
R
Kabra
Sandhya
S
Deshmukh
Pravin S
PS
Jadhav
Sushma D
SD
Thorat
Smita R
SR
Datkar
Sharda R
SR
Mehta
Preeti
P
Ingole
Nayana
N
Mathur
Meenakshi
M
Ramachandran
Anita
A
Haldar
Partha
P
Reddy
D C S
DC
Bachani
Damodar
D
Rao
Sujatha
S
Tripathy
Srikanth P
SP
Paranjape
Ramesh S
RS
D019943:Amino Acid Substitution; D024921:Drug Resistance, Multiple, Viral / Q000187:drug effects / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D017320:HIV Protease Inhibitors / Q000627:therapeutic use; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D017423:Sequence Analysis, RNA; D055815:Young Adult
D017320:HIV Protease Inhibitors; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors