Immunological fingerprinting of group B streptococci: from circulating human antibodies to protective antigens.
Group B streptococcus is one of the most important pathogens in neonates, and causes invasive infections in non-pregnant adults with underlying diseases. Applying a genomic approach that relies on human antibodies we identified antigenic GBS proteins, among them most of the previously published protective antigens. In vitro analyses allowed the selection of conserved candidate antigens that were further evaluated in murine lethal sepsis models using several GBS strains. In active and passive immunization models, we identified four protective GBS antigens, FbsA and BibA, as well as two hypothetical proteins, all shown to contribute to virulence based on gene deletion mutants. These protective antigens have the potential to be components of novel vaccines or targets for passive immune prophylaxis against GBS disease.
Meinke
Andreas L
AL
Intercell AG, Campus Vienna Biocenter 3, 1030 Vienna, Austria.
Senn
Beatrice M
BM
Visram
Zehra
Z
Henics
Tamás Z
TZ
Minh
Duc Bui
DB
Schüler
Wolfgang
W
Neubauer
Christina
C
Gelbmann
Dieter
D
Noiges
Birgit
B
Sinzinger
Jan
J
Hanner
Markus
M
Dewasthaly
Shailesh
S
Lundberg
Urban
U
Hordnes
Knut
K
Masoud
Helga
H
Sevelda
Paul
P
von Gabain
Alexander
A
Nagy
Eszter
E
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D005260:Female; D017353:Gene Deletion; D023281:Genomics; D006801:Humans; D007116:Immunization, Passive; D051379:Mice; D009154:Mutation; D011817:Rabbits; D018805:Sepsis / Q000276:immunology / Q000382:microbiology; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; C499709:FbsA protein, Streptococcus agalactiae; C119204:streptococcal protective antigen