title: Paget's disease of bone is not associated with common polymorphisms in interleukin-6, interleukin-8 and tumor necrosis factor alpha genes.
abstract: BACKGROUND
  Cytokines, specially interleukin (IL)-6, play an important role in the differentiation and activation of osteoclasts and might be involved in osteoblast stimulation in Paget's disease of bone (PDB).
  
  
  OBJECTIVE
  The aim of this study was to investigate the association of polymorphisms in IL-6, IL-8 and tumor necrosis factors-alpha (TNFA) genes among Spanish patients with PDB.
  
  
  METHODS
  We studied four single nucleotide polymorphisms (-174 G>C IL-6, -251 T>A IL-8, -238 G>A TNFA and -308 G>A TNFA) in 172 PDB patients and 150 healthy controls. Distribution of alleles and pro-inflammatory genotypes were studied for association with the presence of the disease and with clinical and laboratory data, as well as the response to bisphosphonate treatment in PDB patients.
  
  
  RESULTS
  We found no statistically significant association between genotype and allele distribution of any of the cytokines polymorphism studied and PDB. No association between the clinical and therapeutic characteristics of PDB and the investigated polymorphism were found.
  
  
  CONCLUSIONS
  This study does not support the hypothesis that the analyzed IL6, IL8 and TNFA polymorphism are associated with PDB.
authors: Servicio de Medicina Interna, RETICEF, Hospital Universitario de Salamanca, Los Montalvos s/n, Salamanca, Spain. lcorral@saludcastillayleon.es
authors: Luis
authors: L
authors: Corral-Gudino
authors: Javier
authors: J
authors: del Pino-Montes
authors: Judit
authors: J
authors: García-Aparicio
authors: Manuel
authors: M
authors: Alonso-Garrido
authors: Rogelio
authors: R
authors: González-Sarmiento
mesh_terms: D000483:Alleles; D005260:Female; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D010001:Osteitis Deformans / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha