title: Chloroform distribution and accumulation by combined inhalation plus oral exposure routes in rats.
abstract: The present investigation was undertaken to determine the distribution and accumulation of chloroform in the blood, liver, kidney and abdominal fat of rats after simultaneous exposure by two routes, inhalation and oral. To distinguish the contribution of each route, unmodified chloroform (CHCl3) was administered by inhalation and deuterated chloroform (CDCl3) was administered orally. Exposure by inhalation and oral administration resulted in CHCl3 and CDCl3 concentrations in the tissues which were significantly higher than when exposure was by either inhalation or oral administration alone. This is the first study to follow the contribution of each of two routes of chloroform exposure on chloroform distribution and accumulation in target tissues. Our results indicate that when assessing the toxicity and carcinogenicity of chloroform, exposure routes, especially the effects of exposure by multiple routes, must be taken into consideration.
authors: Japan Bioassay Research Center, Japan Industrial Safety and Health Association, Hadano, Kanagawa, Japan. m-take@jisha.or.jp
authors: Makoto
authors: M
authors: Take
authors: Seigo
authors: S
authors: Yamamoto
authors: Makoto
authors: M
authors: Ohnishi
authors: Michiharu
authors: M
authors: Matsumoto
authors: Kasuke
authors: K
authors: Nagano
authors: Takashi
authors: T
authors: Hirota
authors: Shoji
authors: S
authors: Fukushima
mesh_terms: D000280:Administration, Inhalation; D000284:Administration, Oral; D000818:Animals; D002725:Chloroform / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats
chemical_list: D002725:Chloroform