Cathepsin D messenger RNA is downregulated in human lung cancer.
OBJECTIVE
Lysosomal proteases cathepsins B and D (CB and CD) play a significant part in cancer progression. For many oncological diseases protein expression levels of CB and CD have been investigated and correlations with tumour characteristics revealed. Meanwhile, there is very little information concerning mRNA expression level.


METHODS
In the present work, data about mRNA levels of CB and CD in human lung cancer was obtained using reverse transcription followed by real-time polymerase chain reaction.


RESULTS
For the first time CD and CB mRNA in human lung cancer tumours was quantified. It was shown that CB and CD mRNA levels do not correlate with any tumour characteristics. However, in most analysed tumours, expression of CD mRNA was downregulated compared with adjacent normal tissue (p <0.0003).


CONCLUSIONS
The data obtained indicate CD mRNA as a potential lung cancer marker.
Shubin
Andrey V
AV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Demidyuk
Ilya V
IV
Kurinov
Alexander M
AM
Demkin
Vladimir V
VV
Vinogradova
Tatyana V
TV
Zinovyeva
Marina V
MV
Sass
Alexander V
AV
Zborovskaya
Irina B
IB
Kostrov
Sergey V
SV
D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002402:Cathepsin D / Q000235:genetics*; D017931:DNA Primers; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D017931:DNA Primers; D012333:RNA, Messenger; D002402:Cathepsin D