title: Decrease of peritoneal inflammatory CD4(+), CD8(+), CD19(+) lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection.
abstract: After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.
authors: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF 04510, Ap. Postal 70-242, Mexico.
authors: Nadia
authors: N
authors: Zepeda
authors: Sandra
authors: S
authors: Solano
authors: Natalia
authors: N
authors: Copitin
authors: Ana María
authors: AM
authors: Fernández
authors: Lilián
authors: L
authors: Hernández
authors: Patricia
authors: P
authors: Tato
authors: José L
authors: JL
authors: Molinari
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010538:Peritonitis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013621:Taenia / Q000302:isolation & purification; D013622:Taeniasis / Q000276:immunology* / Q000469:parasitology / Q000473:pathology*; D013997:Time Factors
chemical_list: D018941:Antigens, CD19