[Methylation status of zo-1 gene promoter in acute leukemia].
This study was purposed to investigate the difference of zo-1 gene promoter methylation between healthy individuals and acute leukemia patients. BS-PCR method was used to detect the status of zo-1 gene methylation in healthy individuals, acute leukemia patients and leukemic cell line NB4 cells. The results showed that zo-1 gene was hypomethylated in bone marrow samples from healthy individuals (1.9%). In newly diagnosed AL and relapsed patients, the rate of zo-1 gene methylation was 93.2% and 66.9% respectively, while it was 16.4% in AL patients in complete remission, which was much higher than that in healthy individuals. There was significant difference between them. It is concluded that as compared with healthy individuals, zo-1 gene in acute leukemia patients is hypermethylated and with different degrees in various phases of leukemia. Analysis of zo-1 gene methylation status may be useful to monitor the development of acute leukemia.
Wang
Xin-Rong
XR
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Gao
Xiao-Ning
XN
Kang
Hui-Yuan
HY
Wang
Li-Li
LL
Li
Yong-Hui
YH
Yu
Li
L
D000208:Acute Disease; D045744:Cell Line, Tumor; D019175:DNA Methylation; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D062826:Zonula Occludens-1 Protein
D008565:Membrane Proteins; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein