title: Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.
abstract: A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.
authors: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Naowarat
authors: N
authors: Tanomsing
authors: Mallika
authors: M
authors: Imwong
authors: Sasikrit
authors: S
authors: Theppabutr
authors: Sasithon
authors: S
authors: Pukrittayakamee
authors: Nicholas P J
authors: NP
authors: Day
authors: Nicholas J
authors: NJ
authors: White
authors: Georges
authors: G
authors: Snounou
mesh_terms: D021901:DNA, Intergenic; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods*; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase