title: Quantification of chemically reducing species in the phosphate ion catalyzed degradation of reducing sugars.
abstract: Chemically reducing species formed during phosphate ion catalyzed degradation of reducing sugars were directly quantified by titration with 2,6-dichloroindophenol (Tillman's reagent) and by measurement of open circuit electrical redox potentials. Both techniques demonstrated a time-dependent increased production of chemically reducing species in 0.1 M phosphate buffer at 100 degrees C and the increasingly negative redox potentials observed were consistent with the formation of reductones. Cyclic voltammetry (CV) was investigated in an attempt to generate and observe the sugar-derived highly reactive reducing species in situ. CV analysis of a model Amadori compound, N-(1-deoxyfructos-1-yl)piperidine, indicated oxidative waves consistent with reductone formation, but chemical instability of the oxidation products formed precluded the electrochemical detection of highly electrophilic reducing species such as reductones.
authors: Department of Chemistry, Miami University Middletown, Middletown, Ohio 45042, USA. georgerizzi@yahoo.com
authors: George P
authors: GP
authors: Rizzi
authors: Eme E
authors: EE
authors: Amba
authors: William R
authors: WR
authors: Heineman
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002384:Catalysis; D010084:Oxidation-Reduction; D010710:Phosphates / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D010710:Phosphates