Enzymatic activity, sensitivity to antifungal drugs and Baccharis dracunculifolia essential oil by Candida strains isolated from the oral cavities of breastfeeding infants and in their mothers' mouths and nipples.
Candida strains can cause oral candidosis, as well as nipples candidosis and lead to premature weaning or yeast transmission. The aim of this study was to evaluate 51 Candida isolates obtained from the oral cavities of infants during breastfeeding and mothers' oral cavities and nipples, their enzymatic activity and their sensitivity to amphotericin B, fluconazole and Baccharis dracunculifolia essential oil. Among the studied strains, 96.1% produced phospholipase and 78.4% produced proteinase. The antifungal resistance was only observed among isolates of C. albicans, for which three strains showed a resistant activity to fluconazole and one showed a resistant activity to amphotericin B. All strains were sensitive to B. dracunculifolia essential oil with MIC between 0.2 and 6.25 mg/ml. It was concluded that most of the strains showed significant enzymatic activity and were sensitive to amphotericin B and fluconazole. B. dracunculifolia essential oil inhibited the growth of all strains, including the ones resistant to commercial antifungal agents.
Pereira
Cristiane Aparecida
CA
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, São José dos Campos Dental School, São Paulo State University (UNESP), Avenida Engenheiro Francisco José Longo, 777, São Dimas, São José dos Campos, CEP 12245-000, São Paulo, Brazil.
da Costa
Anna Carolina Borges Pereira
AC
Machado
Ana Karina Silva
AK
Beltrame Júnior
Miltom
M
Zöllner
Maria Stella Amorin Costa
MS
Junqueira
Juliana Campos
JC
Jorge
Antonio Olavo Cardoso
AO
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D031175:Baccharis / Q000737:chemistry; D001942:Breast Feeding; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D025141:Drug Resistance, Fungal; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D009558:Nipples / Q000382:microbiology*; D009822:Oils, Volatile / Q000494:pharmacology*; D010740:Phospholipases / Q000378:metabolism*
D000935:Antifungal Agents; D005656:Fungal Proteins; D009822:Oils, Volatile; D000666:Amphotericin B; D015725:Fluconazole; D010740:Phospholipases; D010450:Endopeptidases