Method for umbilical cord blood-derived basophils by FCM.
We have devised a method for the purification of human basophils from umbilical cord blood by FCM. Umbilical cord blood was collected from six healthy, full-term deliveries. After separation of red blood cells, mononuclear cells were isolated by Ficoll-Hypaque. Six samples followed by positive selection using flowcytometry (FCM) for CD203c(+)CD45(int+) cells. Purity and recovery of cells were measured. Purity and recovery of basophils by FCM with CD203c(+)CD45(int+) markers were 95.02 +/- 2.94% and 61.42 +/- 5.95%. Cell sorting for CD203c(+)CD45(int+) cells by FCM is an improved method for obtaining pure umbilical cord blood-derived basophils. We established cord blood-derived basophil purification technique as a source from which active basophils can be isolated for immunochemical characterization.
Yang
Ling
L
Respiratory Department, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xu
Wei-Guo
WG
Xu
Yi-Ping
YP
Yao
Su-Hang
SH
Guo
Yin-Shi
YS
Wang
Ke-Min
KM
Xiong
Ying
Y
Zhu
Jie
J
D001491:Basophils / Q000166:cytology* / Q000502:physiology; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology*; D005362:Ficoll / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000378:metabolism; D011755:Pyrophosphatases / Q000276:immunology / Q000378:metabolism
C427944:ENPP3 protein, human; D005362:Ficoll; D017493:Leukocyte Common Antigens; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases