[Identification of sibling sisters using STR and SNP].
OBJECTIVE
The methods for identification of sibling sisters were explored with detection of genetic markers on autochromosome and X-chromosome.


METHODS
Genomic DNA of the sibling sisters were extracted, and 15 STRs on autochromosome and 17 STRs on X-chromosome were genotyped by Sinofiler kit, Mentype Argus X-8 kit and in-house kit of X-STRs, respectively. 11 X-SNPs were genotyped with TaqMan technology.


RESULTS
Full sibling relationship of the test samples were confirmed by calculating full sibling index of STRs in autochromosome, which were also supported by the detection of 1-2 same alleles at each locus on X-chromosome.


CONCLUSIONS
In identification of full sibling sister, not only STRs on autochromosome but also polymorphism genetic markers in X-chromosome can be utilized.
Zhang
Su-Hua
SH
Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zsh-daisy@163.com
Zhao
Shu-Min
SM
Li
Li
L
D000465:Algorithms; D000483:Alleles; D041321:Chromosomes, Human, X / Q000235:genetics*; D004247:DNA / Q000097:blood; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D035781:Siblings; D020080:Tandem Repeat Sequences
D005819:Genetic Markers; D004247:DNA