title: Pre-ischemic treadmill training induces tolerance to brain ischemia: involvement of glutamate and ERK1/2.
abstract: Physical exercise has been shown to be beneficial in stroke patients and animal stroke models. However, the exact mechanisms underlying this effect are not yet very clear. The present study investigated whether pre-ischemic treadmill training could induce brain ischemic tolerance (BIT) by inhibiting the excessive glutamate release and event-related kinase 1/2 (ERK1/2) activation observed in rats exposed to middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were divided into three groups (n = 12/group): sham surgery without prior exercise, MCAO without prior exercise and MCAO following three weeks of exercise. Pre-MCAO exercise significantly reduced brain infarct size (103.1 +/- 6.7 mm3) relative to MCAO without prior exercise (175.9 +/- 13.5 mm3). Similarly, pre-MCAO exercise significantly reduced neurological defects (1.83 +/- 0.75) relative to MCAO without exercise (3.00 +/- 0.63). As expected, MCAO increased levels of phospho-ERK1/2 (69 +/- 5%) relative to sham surgery (40 +/- 5%), and phospho-ERK1/2 levels were normalized in rats exposed to pre-ischemic treadmill training (52 +/- 6%) relative to MCAO without exercise (69% +/- 5%). Parallel effects were observed on striatal glutamate overflow. This study suggests that pre-ischemic treadmill training might induce neuroprotection by inhibiting the phospho-ERK1/2 over-activation and reducing excessive glutamate release.
authors: Department of Rehabilitation Medicine, Hua Shan Hospital, Fudan University, Shanghai, China. zjk20019@126.com
authors: Feng
authors: F
authors: Zhang
authors: Yi
authors: Y
authors: Wu
authors: Jie
authors: J
authors: Jia
authors: Yong-Shan
authors: YS
authors: Hu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D020520:Brain Infarction / Q000150:complications / Q000201:enzymology / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000201:enzymology* / Q000517:prevention & control*; D005080:Exercise Test; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D017072:Neostriatum / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018698:Glutamic Acid; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3