The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson's disease rat model.
This study was designed to assess the potential therapeutic efficacy of gene-modified mesenchymal stem cells (MSCs), MSCs-TH and MSCs-GDNF, in PD rats. Fifty-nine PD rat models were divided into five groups and then the gene-modified MSCs were transplanted into the striatum of rats according to the design. Apomorphine-induced rotational behavior in rats was observed weekly; rats which received both MSCs-TH and MSCs-GDNF showed the most significant improvement compared with those in other groups (P < 0.01). Three weeks later, immunohistochemistry analysis found TH-positive cells and GDNF-positive cells in striatal. Eight weeks later, PD rats were killed. HPLC and ELISA results showed DA and GDNF content in striatum of rats which received both MSCs-TH, and MSCs-GDNF was considerably higher compared with those of other groups (P < 0.01),respectively. In conclusion, our results suggest that combined transplantation of MSCs expressing TH and GDNF can lead to remarkable therapeutic effects in a rat model of PD.
Shi
Dezhi
D
Department of Neurosurgery, Jiangsu Provincial Hospital of Traditional Chinese Medicine, 155 Hanzhong Road, 210029, Nanjing, People's Republic of China.
Chen
Gong
G
Lv
Li
L
Li
Lixin
L
Wei
Dong
D
Gu
Peiyuan
P
Gao
Juemin
J
Miao
Yi
Y
Hu
Weixing
W
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D007839:Functional Laterality; D005818:Genetic Engineering / Q000379:methods*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009043:Motor Activity / Q000502:physiology; D010300:Parkinson Disease / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D051381:Rats; D014161:Transduction, Genetic / Q000379:methods; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D051100:Glial Cell Line-Derived Neurotrophic Factor; D015102:3,4-Dihydroxyphenylacetic Acid; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine