Cells with hematopoietic activity in the mouse placenta reside in side population.
The discovery of a major hematopoietic stem cell pool in midgestation mouse embryo has defined the placenta as an important hematopoietic anatomical site. In this study, we examined the flow cytometric pattern of mouse placenta cells on embryonic days (E) 10.5 to E15.5, in view of CD45 and c-Kit expression. We also determined which population of these cells shows differentiation potential toward multiple hematopoietic lineages by performing coculture with OP9 stromal cells and colony-forming assay in methylcellulose. Only CD45(+)c-Kit(+) population showed the ability to form hematopoietic colonies including multiple lineages. To distinguish which fraction of placenta cells have the hematopoietic activity, we used GFP transgenic mice in which the fetal part of the placenta is GFP positive and the maternal part is GFP negative. E11.5 and E13.5 CD45(+)c-Kit(+) placental cells that have ability to form hematopoietic colonies are the fetal GFP positive placental cells. E11.5 and E13.5 CD45(+)c-Kit(+) placental cells that have an ability to form hematopoietic colonies mainly reside in Hoechst dye-effluxing side population area (SP). Taken together, in the placenta of mouse embryo, we conclude that SP cells in the CD45(+)c-Kit(+) fetal placental cells have the ability to form hematopoietic colonies.
Ramadan
Ahmed
A
Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, Japan.
Nobuhisa
Ikuo
I
Yamasaki
Shoutarou
S
Nakagata
Naomi
N
Taga
Tetsuya
T
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D011247:Pregnancy; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
D018952:Antigens, CD34; D040201:Platelet Membrane Glycoprotein IIb; D049452:Green Fluorescent Proteins; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse