title: Assessment of the expression of IRβ, IRS-1, IRS-2 and IGF-IRβ in a rat model of intrauterine growth restriction.
abstract: OBJECTIVE
  To investigate glomerular development and expression of insulin and insulin-like growth factor receptors in an experimental model of intrauterine growth restriction (IUGR).
  
  
  METHODS
  We studied three groups of Sprague-Dawley fetuses: IUGR - restricted by ligation of the right uterine artery; C-IUGR - left horn controls, and EC - external controls (non-manipulated). Body and organs were weighed, and glomerular number and volume were analyzed. Expression of IRβ, IRS-1, IRS-2 and IGF-IRβ was analyzed in liver, intestine and kidneys by immunoblotting.
  
  
  RESULTS
  Organ/body weight ratios were similar. In IUGR, glomerular number and volume were increased compared to C-IUGR and EC (p<0.001). In the IUGR liver, increases were found in IGF-IRβ compared to C-IUGR and EC; IRβ compared to EC, and IRS-2 compared to C-IUGR. However, decreases in IRβ were noted in IUGR compared to C-IUGR; IRS-1 compared to C-IUGR and EC, and IRS-2 compared to EC. In IUGR intestine, increases were detected in IRβ, IRS-1 and IGF-IRβ compared to C-IUGR and EC. In IUGR kidneys, increases were observed in IRβ and IGF-IRβ compared to C-IUGR and EC, and IRS-1 compared to EC. Decreased IRS-2 in the intestine and kidney were noticed in IUGR compared to C-IUGR and EC.
  
  
  CONCLUSIONS
  IUGR fetuses had less glomeruli and alterations in insulin receptors, which may be associated with an increased risk of disease occurrence in adulthood.
authors: Department of Obstetrics and Gynecology, University of Campinas, Brazil.
authors: Marcia Pereira
authors: MP
authors: Bueno
authors: Dioze
authors: D
authors: Guadagnini
authors: Frances Lilian Lanhellas
authors: FL
authors: Gonçalves
authors: Ricardo
authors: R
authors: Barini
authors: Mario José Abdalla
authors: MJ
authors: Saad
authors: Augusto Frederico
authors: AF
authors: Schmidt
authors: Lourenço
authors: L
authors: Sbragia
mesh_terms: D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D047109:Fetal Development; D005317:Fetal Growth Retardation / Q000378:metabolism* / Q000503:physiopathology; D005333:Fetus / Q000737:chemistry / Q000378:metabolism; D055504:Insulin Receptor Substrate Proteins / Q000032:analysis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry / Q000254:growth & development; D007668:Kidney / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D009929:Organ Size; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin / Q000032:analysis / Q000378:metabolism*; D017451:Receptors, Somatomedin / Q000032:analysis / Q000378:metabolism*
chemical_list: D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; C526299:Irs2 protein, rat; D017451:Receptors, Somatomedin; D011972:Receptor, Insulin