Esterification activity and conformation studies of Burkholderia cepacia lipase in conventional organic solvents, ionic liquids and their co-solvent mixture media.
In this work, experiments were carried out to evaluate the esterification activity and conformation of lipase from Burkholderia cepacia in the selected conventional organic solvents, ionic liquids and their co-solvent mixture media. The results revealed that the activity of esterification of B. cepacia lipase was mostly highest in co-solvent mixture of ionic liquids-organic solvents, followed by conventional organic solvents and ionic liquids. Hence, co-solvent mixture was a high-effective strategy to enhance the activity of B. cepacia lipase for non-aqueous enzymology reaction. Conformational studies via circular dichroism spectroscopy indicated that the secondary structure of B. cepacia lipase was variant in the above-mentioned media, especially the content of alpha-helix, which was probably responsible for lipase activity difference.
Pan
Shaotao
S
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Liu
Xue
X
Xie
Yadong
Y
Yi
Yuyin
Y
Li
Chong
C
Yan
Yunjun
Y
Liu
Yun
Y
D016956:Burkholderia cepacia / Q000187:drug effects / Q000201:enzymology*; D004951:Esterification / Q000187:drug effects; D052578:Ionic Liquids / Q000494:pharmacology*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D009930:Organic Chemicals / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D012997:Solvents / Q000494:pharmacology*
D052578:Ionic Liquids; D009930:Organic Chemicals; D012997:Solvents; D008049:Lipase