Dietary free oleic and linoleic acid enhances neutrophil function and modulates the inflammatory response in rats.
The high ingestion of oleic (OLA) and linoleic (LNA) acids by Western populations, the presence of inflammatory diseases in these populations, and the importance of neutrophils in the inflammatory process led us to investigate the effects of oral ingestion of unesterified OLA and LNA on rat neutrophil function. Pure OLA and LNA were administered by gavage over 10 days. The doses used (0.11, 0.22 and 0.44 g/kg of body weight) were based on the Western consumption of OLA and LNA. Neither fatty acid affected food, calorie or water intake. The fatty acids were not toxic to neutrophils as evaluated by cytometry using propidium iodide (membrane integrity and DNA fragmentation). Neutrophil migration in response to intraperitoneal injection of glycogen and in the air pouch assay, was elevated after administration of either OLA or LNA. This effect was associated with enhancement of rolling and increased release of the chemokine CINC-2alphabeta. Both fatty acids elevated L-selectin expression, whereas no effect on beta(2)-integrin expression was observed, as evaluated by flow cytometry. LNA increased the production of proinflammatory cytokines (IL-1beta and CINC-2alphabeta) by neutrophils after 4 h in culture and both fatty acids decreased the release of the same cytokines after 18 h. In conclusion, OLA and LNA modulate several functions of neutrophils and can influence the inflammatory process.
Rodrigues
Hosana Gomes
HG
Department of Physiology and Biophysics, Institute of Biomedical Sciences, São Paulo University, Avenida Prof. Lineu Prestes, 1524, Butantã, São Paulo, SP, 05508-900, Brazil. hosanagr@icb.usp.br
Vinolo
Marco Aurélio Ramirez
MA
Magdalon
Juliana
J
Fujiwara
Haroldo
H
Cavalcanti
Danielle M H
DM
Farsky
Sandra H P
SH
Calder
Philip C
PC
Hatanaka
Elaine
E
Curi
Rui
R
D000818:Animals; D002448:Cell Adhesion; D016207:Cytokines / Q000096:biosynthesis*; D004032:Diet; D005434:Flow Cytometry; D018836:Inflammation Mediators / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D019787:Linoleic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D019301:Oleic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D016207:Cytokines; D018836:Inflammation Mediators; D019041:L-Selectin; D019301:Oleic Acid; D019787:Linoleic Acid