[Inhibition of Zoledronic Acid on Cell Proliferation and Invasion of Lung Cancer Cell Line 95D.].
BACKGROUND
Abnormal proliferation and metastasis is the basic characteristic of malignant tumors. The aim of this work is to explore the effects of zoledronic acid on cell proliferation and invasion in lung cancer cell line 95D.


METHODS
The effect of zoledrnic acid (ZOL) on proliferation of lung cancer cell line 95D was detected by MTT. The expression of proliferation and invasion-relation genes and proteins were detected by Western blot, RT-PCR and immunofluorescence. Changes of invasion of lung cancer cell numbers were measured by polycarbonates coated with Matrigel.


RESULTS
ZOL could inhibit the proliferation of lung cancer cell line 95D in vitro in a time-dependant and a dose-dependant manner. With time extending after ZOL treated, the mRNA expresion of VEGF, MMP9, MMP2 and protein expression of VEGF, MMP9, ERK1/ERK2 were decreased. The results of Tanswell invasion showed the numbers of invasive cells were significantly reduced in 95D cells treated with ZOL 4 d and 6 d later.


CONCLUSIONS
ZOL could inhibit cell proliferation and invasion of lung cancer cell line 95D.
Li
Mingming
M
Department of Thoracic surgery, Tianjin First Central Hospital, Tianjin 300192, China.
Zhang
Weidong
W
Zong
Zhihong
Z
Hu
Zhenhua
Z
Wang
Xuhui
X