Layer-by-layer assembled multilayers using catalase-encapsulated gold nanoparticles.
We introduce a novel and versatile approach for the preparation of multilayers, based on catalase-encapsulated gold nanoparticles (CAT-Au(NP)), allowing electrostatic charge reversal and structural transformation through pH adjustment. CAT-Au(NP), which are synthesized directly from CAT stabilizer, can be electrostatically assembled with anionic and cationic PEs as a result of the charge reversal of the catalase stabilizers through pH control. In particular, at pH 5.2, near the pI of catalase, dispersed CAT-Au(NP) are structurally transformed into colloidal or network CAT-Au(NP) nanocomposites. Furthermore, we demonstrate that the layer-by-layer assembled multilayers composed of PEs and CAT-Au(NP) induce an effective electron transfer between CAT and the electrode as well as a high loading of CAT and Au(NP), and resultantly exhibit a highly catalytic activity toward H(2)O(2).
Kim
Sungwoo
S
School of Advanced Materials Engineering, Kookmin University, Seoul, Korea.
Park
Jeongju
J
Cho
Jinhan
J
D015374:Biosensing Techniques; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*
D004800:Enzymes, Immobilized; D006046:Gold; D006861:Hydrogen Peroxide; D002374:Catalase