title: Interactions of the apolipoprotein C-III 3238C>G polymorphism and alcohol consumption on serum triglyceride levels.
abstract: BACKGROUND
  Both apolipoprotein (Apo) C-III gene polymorphism and alcohol consumption have been associated with increased serum triglyceride (TG) levels, but their interactions on serum TG levels are not well known. The present study was undertaken to detect the interactions of the ApoC-III 3238C>G (rs5128) polymorphism and alcohol consumption on serum TG levels.
  
  
  METHODS
  A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the ApoC-III 3238C>G was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Interactions of the ApoC-III 3238C>G genotype and alcohol consumption was assessed by using a cross-product term between genotypes and the aforementioned factor.
  
  
  RESULTS
  Serum total cholesterol (TC), TG, high-density lipoprotein cholesterol (HDL-C), ApoA-I and ApoB levels were higher in drinkers than in nondrinkers (P < 0.05-0.001). There was no significant difference in the genotypic and allelic frequencies between the two groups. Serum TG levels in nondrinkers were higher in CG genotype than in CC genotype (P < 0.01). Serum TC, TG, low-density lipoprotein cholesterol (LDL-C) and ApoB levels in drinkers were higher in GG genotype than in CC or CG genotype (P < 0.01 for all). Serum HDL-C levels in drinkers were higher in CG genotype than in CC genotype (P < 0.01). Serum TC, TG, HDL-C and ApoA-I levels in CC genotype, TC, HDL-C, ApoA-I levels and the ratio of ApoA-I to ApoB in CG genotype, and TC, TG, LDL-C, ApoA-I and ApoB levels in GG genotype were higher in drinkers than in nondrinkers (P < 0.05-0.01). But the ratio of ApoA-I to ApoB in GG genotype was lower in drinkers than in nondrinkers (P < 0.01). Multivariate logistic regression analysis showed that the levels of TC, TG and ApoB were correlated with genotype in nondrinkers (P < 0.05 for all). The levels of TC, LDL-C and ApoB were associated with genotype in drinkers (P < 0.01 for all). Serum lipid parameters were also correlated with age, sex, alcohol consumption, cigarette smoking, blood pressure, body weight, and body mass index in both groups.
  
  
  CONCLUSIONS
  This study suggests that the ApoC-III 3238CG heterozygotes benefited more from alcohol consumption than CC and GG homozygotes in increasing serum levels of HDL-C, ApoA-I, and the ratio of ApoA-I to ApoB, and lowering serum levels of TC and TG.
authors: Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China. yinruixing@yahoo.com.cn
authors: Yin
authors: Y
authors: Ruixing
authors: Li
authors: L
authors: Yiyang
authors: Li
authors: L
authors: Meng
authors: Li
authors: L
authors: Kela
authors: Long
authors: L
authors: Xingjiang
authors: Zhang
authors: Z
authors: Lin
authors: Liu
authors: L
authors: Wanying
authors: Wu
authors: W
authors: Jinzhen
authors: Yang
authors: Y
authors: Dezhai
authors: Lin
authors: L
authors: Weixiong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking / Q000097:blood*; D016632:Apolipoprotein A-I / Q000097:blood; D053305:Apolipoprotein C-III / Q000737:chemistry / Q000235:genetics*; D001055:Apolipoproteins B / Q000097:blood; D002681:China / Q000453:epidemiology; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000453:epidemiology / Q000235:genetics / Q000517:prevention & control; D015228:Hypertriglyceridemia / Q000097:blood / Q000453:epidemiology* / Q000235:genetics* / Q000517:prevention & control; D008055:Lipids / Q000097:blood; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013223:Statistics as Topic; D014280:Triglycerides / Q000097:blood*; D055815:Young Adult
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D053305:Apolipoprotein C-III; D001055:Apolipoproteins B; D008055:Lipids; D014280:Triglycerides