[A study for testing the antifungal susceptibility of yeast by the Japanese Society for Medical Mycology (JSMM) method. The proposal of the modified JSMM method 2009].
The Japanese Society for Medical Mycology (JSMM) method used for testing the antifungal susceptibility of yeast, the MIC end point for azole antifungal agents, is currently set at IC(80). It was recently shown, however that there is an inconsistency in the MIC value between the JSMM method and the CLSI M27-A2 (CLSI) method, in which the end- point was to read as IC(50). To resolve this discrepancy and reassess the JSMM method, the MIC for three azoles, fluconazole, itraconazole and voriconazole were compared to 5 strains of each of the following Candida species: C. albicans, C. glabrata, C. tropicalis, C. parapsilosis and C. krusei, for a total of 25 comparisons, using the JSMM method, a modified JSMM method, and the CLSI method. The results showed that when the MIC end- point criterion of the JSMM method was changed from IC(80) to IC(50) (the modified JSMM method) , the MIC value was consistent and compatible with the CLSI method. Finally, it should be emphasized that the JSMM method, using a spectrophotometer for MIC measurement, was superior in both stability and reproducibility, as compared to the CLSI method in which growth was assessed by visual observation.
Nishiyama
Yayoi
Y
Teikyo University Institute of Medical Mycology.
Abe
Michiko
M
Ikeda
Reiko
R
Uno
Jun
J
Oguri
Toyoko
T
Shibuya
Kazutoshi
K
Maesaki
Shigefumi
S
Mohri
Shinobu
S
Yamada
Tsuyoshi
T
Ishibashi
Hiroko
H
Hasumi
Yayoi
Y
Abe
Shigeru
S
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D007564:Japan; D008826:Microbial Sensitivity Tests / Q000592:standards*; D009172:Mycology; D012955:Societies, Medical
D000935:Antifungal Agents