Silencing of SlFTR-c, the catalytic subunit of ferredoxin:thioredoxin reductase, induces pathogenesis-related genes and pathogen resistance in tomato plants.
As a heterodimeric protein, ferredoxin:thioredoxin reductase (FTR) catalyses the light-dependant activation of several photosynthetic enzymes. The active site of the catalytic subunit of FTR contains a redox-active disulfide and a [4Fe-4S] center. We isolated the catalytic subunit gene of FTR, designated SlFTR-c, from tomato (Solanum lycopersicum L.). SlFTR-c transcripts were detected in all tissues examined, including roots, leaves, flowers, fruits, and seeds. Interestingly, virus-induced gene silencing (VIGS) of SlFTR-c resulted in necrotic lesions with typical cell death symptoms and reactive oxygen species (ROS) production in tomato leaves. Moreover, these SlFTR-c-silenced plants displayed enhanced disease resistance against bacterial pathogens, specifically Pseudomonas syringae pv. tomato DC3000, by the induction of defense-related genes (SlPR-1, SlPR-2, SlPR-5, SlGlucA, SlChi3, and SlChi9). Taken together, it seems that SlFTR-c works as a regulator of programmed cell death (PCD) and pathogen resistance in tomato plants.
Lim
Chan Ju
CJ
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
Kim
Woong Bom
WB
Lee
Bok-Sim
BS
Lee
Ha Youn
HY
Kwon
Tae-Ho
TH
Park
Jeong Mee
JM
Kwon
Suk-Yoon
SY
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D018551:Solanum lycopersicum / Q000166:cytology / Q000235:genetics* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D044224:Pseudomonas syringae; D014158:Transcription, Genetic
D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C021588:ferredoxin-thioredoxin reductase