title: Demonstration of conjugative transposon (Tn5397)-mediated horizontal gene transfer between Clostridium difficile and Enterococcus faecalis.
abstract: Antibiotic-resistant Enterococcus faecalis and Clostridium difficile are responsible for nosocomial infections in humans, in which they inhabit the same niche. Here, we demonstrate transfer of the conjugative transposon Tn5397 from C. difficile 630 to E. faecalis JH2-2, the first reported gene transfer between these two bacteria. Furthermore, transfer from the E. faecalis EF20A transconjugant to the epidemic ribotype 027 C. difficile strain R20291 was also demonstrated. Tn5397 was shown to use a single specific target site in E. faecalis; it also has specific target sites in C. difficile. These experiments highlight the importance of continual monitoring for emerging resistances in these bacteria.
authors: Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom.
authors: Azmiza S
authors: AS
authors: Jasni
authors: Peter
authors: P
authors: Mullany
authors: Haitham
authors: H
authors: Hussain
authors: Adam P
authors: AP
authors: Roberts
mesh_terms: D016360:Clostridioides difficile / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics*; D022761:Gene Transfer, Horizontal / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements