Rapid selection of transgenic C. elegans using antibiotic resistance.
Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
Semple
Jennifer I
JI
European Molecular Biology-Centre for Genomic Regulation Systems Biology Unit, UPF, Barcelona, Spain.
Garcia-Verdugo
Rosa
R
Lehner
Ben
B
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics*; D004351:Drug Resistance / Q000187:drug effects* / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D011691:Puromycin / Q000494:pharmacology; D012641:Selection, Genetic / Q000187:drug effects*; D013997:Time Factors; D019076:Transgenes / Q000235:genetics*
D005819:Genetic Markers; D011691:Puromycin