title: Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens.
abstract: A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.
authors: Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom. lewis.white@wales.nhs.uk
authors: P Lewis
authors: PL
authors: White
authors: Michael D
authors: MD
authors: Perry
authors: Juergen
authors: J
authors: Loeffler
authors: Willem
authors: W
authors: Melchers
authors: Lena
authors: L
authors: Klingspor
authors: Stephane
authors: S
authors: Bretagne
authors: Elaine
authors: E
authors: McCulloch
authors: Manuel
authors: M
authors: Cuenca-Estrella
authors: Niklas
authors: N
authors: Finnstrom
authors: J Peter
authors: JP
authors: Donnelly
authors: Rosemary A
authors: RA
authors: Barnes
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics*; D001769:Blood / Q000382:microbiology*; D004271:DNA, Fungal / Q000302:isolation & purification*; D006801:Humans; D009172:Mycology / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal