Characterization of protein in old myocardial infarction by FTIR micro-spectroscopy.
The aim of the present study was to assess whether Fourier transform infrared spectrometry (FTIR) micro-spectroscopy could produce distinct spectral information on protein of old myocardial infarction (OMI) and to set them as molecular markers to diagnose atypical OMI. Paraffin-embedded heart samples were derived from victims dying of OMI. In combination with histological stain, FTIR and infrared micro-spectroscopy, the characteristics of OMI were analyzed morphologically and molecularly. The most relevant bands identified were the amide A, B, I and, II showing crucial spectral differences between apparent normal region and OMI region, including the peak position blue shift and the increased intensity of OMI, moreover relative increase in alpha-helix and decrease in beta-sheet of protein secondary structures in OMI. Comparing to single spectral band, the I1650/I1550 ratio was increased and rationally used as a molecular marker for diagnosing OMI. These novel preliminary findings supported further exploration of FTIR molecular profiling in clinical or forensic study, and were in accordance with histopathology.
Zheng
Na
N
Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. nana96069@163.com
Yang
Tiantong
T
Liang
Man
M
Zhang
Haidong
H
Li
Liping
L
Sunnassee
Ananda
A
Liu
Liang
L
D049429:Forensic Pathology / Q000379:methods; D006801:Humans; D008968:Molecular Conformation; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D011506:Proteins