Increasing hybridoma viability and antibody repertoire after the cell fusion by the use of human plasma as an alternative supplement.
Prion diseases such as Bovine Spongiform Encephalopathy (BSE) and new variant Creutzfeldt-Jakob disease (nvCJD) have caused a major safety concern in cell cultures using fetal calf serum (FCS). In this study, we found that screened and tested human plasma (HP) obtained from blood centers may be an ideal alternate nutrient substitute to FCS for culturing hybridoma. In addition to the inherent safety, a ten-fold increase in the fusion efficiency has been observed if the HP was used as the nutrient supplement instead of FCS. Subsequently, a broader antibody repertoire may be recovered. The HP supplement was found to promote the growth of hybridoma cells but no impact on antibody secretion. Interestingly, this effect of enrichment was only observed for HP, but not plasma from other animals. Unidentified murine hybridoma cloning factors other than IL-6 may specifically reside in human blood.
Lee
Ren-Jeh
RJ
Department of Life Science, Catholic Fu-Jen University, Taipei, Taiwan.
Wu
Jiann-Shing
JS
Juang
Rong-Huay
RH
Wu
Yu-Jen
YJ
Lin
Ching-Yu
CY
Wu
Szu-Yu
SY
Huang
Pei-Ru
PR
Chu
Chi-Hong
CH
Chin
Li-Te
LT
Chen
Han-Min
HM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods*; D002459:Cell Fusion / Q000379:methods*; D049109:Cell Proliferation; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010949:Plasma / Q000378:metabolism*
D000911:Antibodies, Monoclonal