title: Rapid selection of transgenic C. elegans using antibiotic resistance.
abstract: Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
authors: European Molecular Biology-Centre for Genomic Regulation Systems Biology Unit, UPF, Barcelona, Spain.
authors: Jennifer I
authors: JI
authors: Semple
authors: Rosa
authors: R
authors: Garcia-Verdugo
authors: Ben
authors: B
authors: Lehner
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics*; D004351:Drug Resistance / Q000187:drug effects* / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D011691:Puromycin / Q000494:pharmacology; D012641:Selection, Genetic / Q000187:drug effects*; D013997:Time Factors; D019076:Transgenes / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D011691:Puromycin