Physicochemical characteristics associated with transfection of cationic cholesterol-based gene delivery vectors in the presence of DOPE.
The physicochemical properties of a novel series of cholesterol-based cationic lipids in the presence of DOPE were studied by various techniques in an effort to correlate cationic lipid structure with transfection efficacy. It was found that while DOPE improves the β-gal activity of the active AC and MC derivatives, the overall zeta potential of the particles, pDNA complexation and condensation is not improved. This is in stark contrast with the tertiary amine derivative DC whose dispersion properties were improved and its monolayer surface potential is restored at high molecular surface density in the presence of DOPE. Overall the transfection activity mediated by DC and the quaternary ammonium TC derivative was greatly improved in the presence of DOPE and is attributed to decreased cytotoxicity, improved fusogenicity and cellular association.
Kearns
Molinda D
MD
Long Island University, Brooklyn, NY 11201, USA.
Patel
Yesha N
YN
Savva
Michalakis
M
D000818:Animals; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D002784:Cholesterol / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002412:Cations; D010714:Phosphatidylethanolamines; D002784:Cholesterol