title: Molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from South India.
abstract: Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala-->Gly) of pncA.
authors: State TB Training and Demonstration Centre, Govt. Hospital for Chest Diseases, Puducherry, India.
authors: Muthuraj
authors: M
authors: Muthaiah
authors: Sridharan
authors: S
authors: Jagadeesan
authors: Nisha
authors: N
authors: Ayalusamy
authors: Manupriya
authors: M
authors: Sreenivasan
authors: Sambamurthy Sangamesvara
authors: SS
authors: Prabhu
authors: Usharani
authors: U
authors: Muthuraj
authors: Kamatchiyammal
authors: K
authors: Senthilkumar
authors: Saroja
authors: S
authors: Veerappan
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism; D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D011718:Pyrazinamide / Q000494:pharmacology* / Q000627:therapeutic use; D014376:Tuberculosis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide; D000581:Amidohydrolases; C022490:pyrazinamide deamidase