title: Electrochromatographic characterization of methacrylate ester-based monolith and capillary electrochromatography separation of flavonoids.
abstract: A porous polymethacrylate ester-based monolithic column for capillary electrochromatography (CEC) was designed by mean of in situ co-polymerizing lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in a ternary porogenic solvent including cyclohexanol, 1,4-butanediol and water. After investigating the influence factors of the CEC monolithic columns, four flavonoids (i.e., Rutin, Quercetin, Kaempferol, and Quercitrin) were separated and assayed to evaluate this monolithic column with CEC method. Under optimum conditions, the CEC method exhibited high separation efficiency, with rapid separation time of 3-4 min, for the four flavonoid samples using 10mM phosphate buffer containing 70% acetonitrile (pH 9.0). Importantly, the proposed method could provide a promising approach for rapid separation and detection in biomedicine.
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, China.
authors: Zongbao
authors: Z
authors: Chen
authors: Yan
authors: Y
authors: Cai
authors: Jintian
authors: J
authors: Cheng
authors: Lan
authors: L
authors: Zhang
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D004355:Drug Stability; D044948:Flavonols / Q000032:analysis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010710:Phosphates; D011109:Polymethacrylic Acids / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000097:Acetonitriles; D044948:Flavonols; D010710:Phosphates; D011109:Polymethacrylic Acids; C018279:sodium phosphate; C032159:acetonitrile