title: CD62L on neutrophil granulocytes, a useful, complementary marker for the prediction of ventriculitis in blood-containing CSF.
abstract: OBJECTIVE
  Presence of residual blood is a common problem in cerebrospinal fluid (CSF) diagnostics of ventriculitis. We hypothesised that neutrophil granulocytes in infected, blood-containing CSF lose CD62L expression. Therefore CD62L expression on neutrophils may present a complementary marker to distinguish between patients with residual blood and infection.
  
  
  METHODS
  Evaluation was performed in 64 ventricular CSF samples sent to the laboratory for diagnostic investigation. Cell count, microbiological culture, total protein and flow cytometric analysis of CSF were performed.
  
  
  RESULTS
  Cell counts and CD62L expression were significantly different between the culture positive and negative group. ROC-analysis revealed a significant predictive value for cell count and CD62L expression. Optimal cut-offs were calculated and a decision tree was established to predict a positive culture.
  
  
  CONCLUSIONS
  Cell count and CD62L expression were predictive for a positive culture and the combination helped to increase specificity and sensitivity for the detection of ventriculitis in blood-containing CSF.
authors: Institut für Klinische Chemie und Labordiagnostik, Friedrich-Schiller University Jena, Jena, Germany. kboeer@med.uni-jena.de
authors: Klas
authors: K
authors: Boer
authors: Heinz
authors: H
authors: Vogelsang
authors: Thomas
authors: T
authors: Deufel
authors: Wolfgang
authors: W
authors: Pfister
authors: Michael
authors: M
authors: Kiehntopf
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D002478:Cells, Cultured; D058565:Cerebral Ventriculitis / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D006801:Humans; D019041:L-Selectin / Q000378:metabolism*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D019041:L-Selectin