title: [Screening and Identification of Non-Small-Cell Lung Cancer Associated Antigen with SEREX.].
abstract: BACKGROUND
  Currently, there are only limited number of diagnostic tumor markers for non-small-cell lung cancer (NSCLC). The aim of this study is to screen and identify non-small-cell lung cancer associated antigens, by using of cancer sera containing antibodies which react with autologous cellular antigens (tumor-associated antigens TAAs), which can be used in NSCLC early screening or target treatment.
  
  
  METHODS
  Two T7 phage display cDNA libraries were panning against the sera from 24 patients with NSCLC. Then the enriched libraries were screened with the pool of sera from NSCLC patients, the positive clones encoding antigenic genes were obtained after screening, and the nucleotide sequences of positive were identified and analyzed with BLAST software in GenBank.
  
  
  RESULTS
  After immunoscreen two T7 phage display cDNA libraries with serum from patients with NSCLC, thirty-one positive clones were obtained and sequence results showed they were derive from 15 genes, 12 of 15 genes were homologous to the genes known in GenBank, such as RHOA, ITGB4, and HNRNPA2/B1 et al . The other three genes expressed sequence tags (ESTs) were not found in GenBank.
  
  
  CONCLUSIONS
  Fifteen NSCLC associated antigen genes and three candiated gene were obtained by SEREX from T7 phage display cDNA libraries.
authors: Department of Cellular and Molecular Biology, Beijing TB and Thoracic Tumor Research Institute, Beijing 101149, China. yuewt2000@yahoo.com.
authors: Wentao
authors: W
authors: Yue
authors: Lina
authors: L
authors: Zhang
authors: Hua
authors: H
authors: Zheng
authors: Yue
authors: Y
authors: Wang
authors: Ying
authors: Y
authors: Sun
authors: Zhenfeng
authors: Z
authors: Jiao