Direct electron transfer of glucose oxidase and biosensing of glucose on hollow sphere-nanostructured conducting polymer/metal oxide composite.
A hollow sphere-nanostructured conductive polymer/metal oxide composite was synthesized and used to investigate the electrochemical behavior of glucose oxidase, demonstrating a significantly enhanced direct electron transfer ability of glucose oxidase. In particular, the long-standing puzzle of whether enzymatic glucose sensing involves an enzyme direct electron transfer process was studied. The results indicate the mechanism is indeed a glucose oxidase direct electron transfer process with competitive glucose oxidation and oxygen reduction to detect glucose. A glucose biosensor with the glucose oxidase-immobilized nanomaterial was further constructed, demonstrating superior sensitivity and reliability, and providing great potential in clinical applications.
Guo
Chun Xian
CX
Center for Advanced Bionanosystems and School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.
Li
Chang Ming
CM
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D008670:Metals / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D008670:Metals; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose