Enhanced uptake of porous silica microparticles by bifunctional surface modification with a targeting antibody and a biocompatible polymer.
Strategies were developed by which mesoporous microparticles were modified on their external surfaces with tetraethylene glycol (TEG), a protein, or both, leaving the pore surfaces available for modification with a separate moiety, such as a dye. Only particles bifunctionally modified with both TEG and a cell-specific antibody were taken up specifically by a targeted cancer cell line. In contrast to similarly functionalized nanoparticles, endocytosed microparticles were not contained within a lysosome.
Cheng
Kai
K
Department of Chemistry, University of Vermont, Burlington, Vermont 05405, USA.
Blumen
Steven R
SR
MacPherson
Maximilian B
MB
Steinbacher
Jeremy L
JL
Mossman
Brooke T
BT
Landry
Christopher C
CC
D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D001672:Biocompatible Materials / Q000737:chemistry*; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000138:chemical synthesis*; D006801:Humans; D008863:Microspheres; D009369:Neoplasms / Q000737:chemistry* / Q000276:immunology*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D004337:Drug Carriers; D011108:Polymers; D012822:Silicon Dioxide