Partial sequence analysis of the L1 gene of bovine papillomavirus type 1 detected by PCR with MY09/MY11 primers in equine sarcoids in Poland.
BPV-1 is now recognized as a main etiological agent of equine sarcoids. The etiopathogenesis of the equine sarcoids is equivocal and is not yet fully understood. The aim of the present study was to analyse a partial sequence of the L1 gene of BPV associated with equine sarcoids in Polish horses. After clinical diagnosis, 40 skin lesions obtained from 29 horses were collected. The amplicons of a fragment of BPV L1 DNA were detected using PCR with MY09/MY11 primers in 31 specimens. All of them were recognized as BPV-1. Phylogenetic analysis has allowed the amplicons of partial L1 gene to be divided into 3 phylogenetic groups (A, B, C) and one separate isolate (20c). Sequence variants from phylogenetic groups B, C and isolate 20c represented new genetic variants of BPV-1 L1. Sequence variants from groups B and C were submitted to GenBank NCBI.
Szczerba-Turek
A
A
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland. a.szczerba@uwm.edu.pl
Siemionek
J
J
Wasowicz
K
K
Szweda
W
W
Raś
A
A
Platt-Samoraj
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000302:isolation & purification*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012507:Sarcoidosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
D036022:Capsid Proteins; C085217:L1 protein, Bovine papillomavirus