Effect of antioxidant vitamins A, C, E and their analogues on azo-dye binding protein in liver of rats treated with p-dimethylaminoazobenzene.
p-Dimethylaminoazobenzene (DAB) is an azo-dye and known to cause liver tumour in rats. Azo-dye binding protein is a specific cytosolic protein involved in the translocation of azo-dye carcinogen metabolites from liver cytoplasm into the nucleus. Administration of vitamin A (40,000 and 50,000 IU), L-ascorbic acid (500 and 1000 mg) and vitamin E succinate (200-500 mg) reduced the amount of azo-dye binding protein in liver of rats treated with DAB. Supplementation of high doses of vitamin A acetate, vitamin A palmitate, sodium ascorbate, ascorbyl palmitate and vitamin E acetate had no effect on the quantity of azo-dye binding protein in liver. When the vitamin mixture was given, the level of azo-dye binding protein decreased in the liver at all the studied doses, which may be due to their synergistic effect.
Velanganni
A Antony Joseph
AA
Department of Biochemistry, J. J. College of Arts and Science, Pudukkottai 622 422, India. ajvelanganni@gmail.com
Balasundaram
C
C
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004396:Coloring Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D003600:Cytosol / Q000378:metabolism; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*; D004124:p-Dimethylaminoazobenzene / Q000493:pharmacokinetics / Q000633:toxicity*
D000975:Antioxidants; D002352:Carrier Proteins; D004396:Coloring Agents; D014815:Vitamins; D014801:Vitamin A; D014810:Vitamin E; D004124:p-Dimethylaminoazobenzene; D001205:Ascorbic Acid