title: Involvement of prostaglandin F2α in preeclamptic human umbilical vein vasospasm: a role of prostaglandin F and thromboxane A2 receptors.
abstract: OBJECTIVE
  Preeclampsia is characterized by hypertension and proteinuria developing after 20 weeks of gestation. Increased vasoconstriction can be one of the major underlying pathophysiological event in this syndrome. We examined the role of vasoconstrictor prostanoid, prostaglandin F2α (PGF2α) in preeclamptic and normotensive human umbilical veins.
  
  
  METHODS
  Umbilical veins were set up in organ bath. The concentration-response curves of PGF2α (endogenous agonist of prostaglandin F receptor) and fluprostenol (prostaglandin F receptor selective agonist) were determined in normal and preeclamptic veins either in the absence or presence of BAY u3405 (thromboxane A2 receptor selective antagonist). PGF2α and its major metabolite concentrations were measured by enzyme immunoassay kit. The expression of vasoconstrictor prostanoid receptors was determined by western blot.
  
  
  RESULTS
  The concentration-dependent contractions to PGF2α and fluprostenol were significantly increased in umbilical vein preparations derived from preeclamptic women compared with those of normotensives. Increased reactivity was related with enhanced sensitivity to these spasmogens in preeclamptic veins. BAY u3405 (10 μmol/l) did not modify the responsiveness to PGF2α in normal umbilical veins whereas moderately reduced the contractions in preeclamptic preparations. Serum concentrations of PGF2α and its major metabolite, 13,14-dihydro-15-keto-PGF2α, were comparable between preeclamptics and normotensives whereas the metabolite concentration was elevated in umbilical cord serum of preeclamptics. 13,14-dihydro-15-keto-PGF2α, release was also increased in umbilical vein preparations of preeclamptic women. An increased prostaglandin F receptor protein expression was determined whereas EP3 and thromboxane A2 protein expressions were unchanged in preeclamptic umbilical veins.
  
  
  CONCLUSIONS
  Prostaglandin F and thromboxane A2 receptors activation by PGF2α could be involved in umbilical vasospasm observed in preeclampsia.
authors: Istanbul University, Faculty of Pharmacy, Department of Pharmacology, Istanbul, Turkey. gokce_topal@hotmail.com
authors: Gokce
authors: G
authors: Topal
authors: Nabil
authors: N
authors: Foudi
authors: B Sonmez
authors: BS
authors: Uydes-Dogan
authors: Thierry
authors: T
authors: Cachina
authors: Mine
authors: M
authors: Kucur
authors: Altay
authors: A
authors: Gezer
authors: Riza
authors: R
authors: Madazli
authors: Osman
authors: O
authors: Ozdemir
authors: Xavier
authors: X
authors: Norel
mesh_terms: D000328:Adult; D015153:Blotting, Western; D015237:Dinoprost / Q000819:agonists / Q000502:physiology*; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D044244:Receptors, Thromboxane A2, Prostaglandin H2 / Q000502:physiology*; D014471:Umbilical Veins / Q000503:physiopathology*
chemical_list: D044244:Receptors, Thromboxane A2, Prostaglandin H2; D015237:Dinoprost