title: A proteomics approach to study synergistic and antagonistic interactions of the fungal-bacterial consortium Fusarium oxysporum wild-type MSA 35.
abstract: Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multispecies consortium that consists of a fungus and numerous rhizobacteria mainly belonging to gamma-proteobacteria, was analyzed by two complementary metaproteomic approaches (2-DE combined with MALDI-Tof/Tof MS and 1-D PAGE combined with LC-ESI-MS/MS) to identify fungal or bacterial factors potentially involved in antagonistic or synergistic interactions between the consortium members. Moreover, the proteome profiles of F. oxysporum WT MSA 35 and its cured counter-part CU MSA 35 (WT treated with antibiotics) were compared with unravel the bacterial impact on consortium functioning. Our study presents the first proteome mapping of an antagonistic F. oxysporum strain and proposes candidate proteins that might play an important role for the biocontrol activity and the close interrelationship between the fungus and its bacterial partners.
authors: Agroinnova-Centre of Competence for the Innovation in the Agro-Environmental Field, University of Torino, Torino, Italy. m.moretti@ivv.cnr.it
authors: Marino
authors: M
authors: Moretti
authors: Alexander
authors: A
authors: Grunau
authors: Daniela
authors: D
authors: Minerdi
authors: Peter
authors: P
authors: Gehrig
authors: Bernd
authors: B
authors: Roschitzki
authors: Leo
authors: L
authors: Eberl
authors: Angelo
authors: A
authors: Garibaldi
authors: Maria Lodovica
authors: ML
authors: Gullino
authors: Kathrin
authors: K
authors: Riedel
mesh_terms: D005656:Fungal Proteins / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry*; D059013:Microbial Consortia; D040901:Proteomics
chemical_list: D005656:Fungal Proteins