Effects of reproductive stage and 11-ketotestosterone on LPL mRNA levels in the ovary of the shortfinned eel.
To understand the dynamics of lipid uptake into the ovary and the potential role that lipoprotein lipase plays in this event, changes in LPL transcript abundance during oogenesis were measured in both wild-caught and pituitary homogenate-induced artificially maturing eels. Also, the effects of 11-ketotestosterone (11-KT) on LPL mRNA levels were investigated in vivo and in vitro. Normalized ovarian LPL transcript abundance increased as oogenesis advanced, and it rose particularly rapidly during midvitellogenesis, corresponding to pronounced increases in ovarian lipid deposits and LPL activity. Furthermore, LPL mRNA levels were dramatically increased following 11-KT treatment in vivo, findings that were reinforced as trends in ovarian tissue incubated in vitro. Ovarian LPL appears to be directly involved in the uptake of lipids into the eel ovary, an involvement that appears to be controlled, at least in part, by the androgen 11-KT.
Divers
Sean L
SL
Department of Zoology, University of Otago, Dunedin, New Zealand.
McQuillan
H James
HJ
Matsubara
Hajime
H
Todo
Takashi
T
Lokman
P Mark
PM
D000811:Anguilla / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D066298:In Vitro Techniques; D050356:Lipid Metabolism / Q000187:drug effects; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000502:physiology*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology
D018076:DNA, Complementary; D012333:RNA, Messenger; D013739:Testosterone; D008071:Lipoprotein Lipase; C003600:11-ketotestosterone