title: A subtype of a Pseudomonas aeruginosa cystic fibrosis epidemic strain exhibits enhanced virulence in a murine model of acute respiratory infection.
abstract: BACKGROUND
  The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is a particularly successful cystic fibrosis (CF) pathogen associated with transmissibility, increased patient morbidity, and, unusually, infection of the non-CF parents of a patient with CF.
  
  
  METHODS
  Using assays for virulence-associated exoproducts, biofilm formation, Caenorhabditis elegans killing, and a murine model of acute respiratory infection, we compared the pathogenic behavior of representatives of 4 subtypes of the LES, including LES431, an isolate associated with the infection of a parent without CF.
  
  
  RESULTS
  The quorum-sensing-defective lasR mutant LES400 produced less exoproduct and had less C. elegans killing activity than the other LES subtypes, which were represented by LES431, LESB58, and LESB65. LES431 was deficient in biofilm formation, compared with the other LES sub-types. The LES subtypes displayed a range of virulence in the mouse model, with LES431 being by far the most virulent. The genome-sequenced isolate LESB58, effective at establishing infections in a rat model of chronic infection, was the least virulent subtype in the murine acute infection model.
  
  
  CONCLUSIONS
  LES isolates display widely variable pathogenic characteristics. LES431, associated with transmission to the non-CF parent of a CF patient, represents a "hypervirulent" subtype more adapted to acute infections than chronic infections.
authors: Department of Infection, Immunity, and Infection, University of Leicester, Leicester, United Kingdom.
authors: Melissa E K
authors: ME
authors: Carter
authors: Joanne L
authors: JL
authors: Fothergill
authors: Martin J
authors: MJ
authors: Walshaw
authors: Kumar
authors: K
authors: Rajakumar
authors: Aras
authors: A
authors: Kadioglu
authors: Craig
authors: C
authors: Winstanley
mesh_terms: D000818:Animals; D018441:Biofilms / Q000254:growth & development; D017173:Caenorhabditis elegans / Q000382:microbiology; D003550:Cystic Fibrosis / Q000150:complications*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D012141:Respiratory Tract Infections / Q000382:microbiology*; D016019:Survival Analysis; D014774:Virulence