Polycationic protamine for water-insoluble complex formation with DNA.
The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.
Fukushima
Tadao
T
Department of Dental Engineering, Bioengineering section, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan. tadaof@college.fdcnet.ac.jp
Ohno
Jun
J
Hayakawa
Tohru
T
Imayoshi
Rieko
R
Kawaguchi
Minoru
M
Doi
Yutaka
Y
Kanaya
Keiichi
K
Mitarai
Makoto
M
D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018736:DNA Adducts / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D020007:Implants, Experimental; D008297:Male; D008422:Materials Testing; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects; D016062:Porosity; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D011479:Protamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012474:Salmon; D012995:Solubility; D013211:Staphylococcus aureus / Q000187:drug effects; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology; D013499:Surface Properties; D014867:Water
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D018736:DNA Adducts; D011479:Protamines; D014867:Water; D004247:DNA