Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties.
Reactive oxygen species (ROS) function in a range of physiological processes such as growth, metabolism and signaling, and also have a pathological role. Recent research highlighted the requirement for ROS generated by dual oxidase (DUOX) in host-defence responses in innate immunity and inflammatory disorders such as inflammatory bowel disease (IBD), but in vivo evidence to support this has, to date, been lacking. In order to investigate the involvement of Duox in gut immunity, we characterized the zebrafish ortholog of the human DUOX genes. Zebrafish duox is highly expressed in intestinal epithelial cells. Knockdown of Duox impaired larval capacity to control enteric Salmonella infection.
Flores
Maria Vega
MV
Department of Molecular Medicine & Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand. m.flores@auckland.ac.nz
Crawford
Katie C
KC
Pullin
Lisa M
LM
Hall
Christopher J
CJ
Crosier
Kathryn E
KE
Crosier
Philip S
PS
D000818:Animals; D055785:Gene Knockdown Techniques; D007413:Intestinal Mucosa / Q000201:enzymology*; D007814:Larva / Q000201:enzymology; D019255:NADPH Oxidases / Q000145:classification / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism; D012486:Salmonella typhimurium; D015027:Zebrafish / Q000378:metabolism* / Q000382:microbiology
D017382:Reactive Oxygen Species; D019255:NADPH Oxidases