Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice.
The Mouse Metabolic Phenotyping Center (MMPC) Consortium was established to address the need to characterize the growing number of mouse models of metabolic diseases, particularly diabetes and obesity. A goal of the MMPC Consortium is to propose standard methods for assessing metabolic phenotypes in mice. In this article, we discuss issues pertaining to the design and performance of various tests of glucose metabolism. We also propose guidelines for the description of methods, presentation of data and interpretation of results. The recommendations presented in this article are based on the experience of the MMPC Consortium and other investigators.
Ayala
Julio E
JE
Vanderbilt-NIH Mouse Metabolic Phenotyping Center, Nashville, TN 37232, USA. jayala@sanfordburnham.org
Samuel
Varman T
VT
Morton
Gregory J
GJ
Obici
Silvana
S
Croniger
Colleen M
CM
Shulman
Gerald I
GI
Wasserman
David H
DH
McGuinness
Owen P
OP
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002404:Catheterization; D003940:Diagnostic Techniques, Endocrine / Q000592:standards*; D005215:Fasting / Q000097:blood; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006706:Homeostasis; D006943:Hyperglycemia / Q000097:blood / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D010641:Phenotype
D001786:Blood Glucose; D007328:Insulin; D005947:Glucose