Elevated plasma osteopontin level is associated with pelvic inflammatory disease.
Our purpose here was to detect the association among plasma osteopontin concentration, single nucleotide polymorphisms (SNPs) of osteopontin gene, and pelvic inflammatory disease (PID). The enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were respectively used to measure the plasma osteopontin level and its gene polymorphisms. The level of plasma osteopontin was elevated in patients with PID as compared to that of healthy women and decreased significantly after treatment. Plasma osteopontin concentration was significantly correlated with white blood cell (WBC) and neutrophil counts and plasma C-reactive protein (CRP) level in patients with PID. No significant difference was found in the genotypes or alleles distribution of osteopontin SNPs, rs1126616 or rs9138, between patients with PID and normal controls. Plasma osteopontin concentration was not associated with osteopontin polymorphism. When the cutoff level of the plasma osteopontin concentration was set to be 58.53 ng/mL, the adjusted odds ratio of plasma osteopontin for PID risk was 3.87 (95% confident interval: 1.30-11.51). Plasma osteopontin level may act as a prediction marker for PID.
Wang
Po-Hui
PH
Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Liu
Yu-Fan
YF
Tsai
Hsiu-Ting
HT
Tee
Yi-Torng
YT
Lin
Long-Yau
LY
Hsieh
Yi-Hsien
YH
Yang
Shun-Fa
SF
D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053495:Osteopontin / Q000097:blood*; D000292:Pelvic Inflammatory Disease / Q000097:blood* / Q000175:diagnosis* / Q000188:drug therapy; D012680:Sensitivity and Specificity
D015415:Biomarkers; C506895:SPP1 protein, human; D053495:Osteopontin