Expression of micro-RNA-145 is regulated by a highly conserved genomic sequence 3' to the pre-miR.
Micro-RNA-145 (miR145), a tumor suppressor miR, dramatically inhibits growth of cancer cells in culture and plays a significant role in human stem cells differentiation. We have isolated a human genomic sequence of 864 bp comprising the pre-miR and its flanking sequences. The cloned miR145 genomic sequence expresses a mature miR145 in transfected cells. We show here that flanking sequences on either side of the pre-miR sequence can modulate its expression levels. Surprisingly, a highly conserved sequence 3' to the pre-miR plays a crucial role in miR145 expression.
La Rocca
Gaspare
G
Department of Cancer Biology, Thomas Jefferson University and Kimmel Cancer Center, Philadelphia, Pennsylvania 19107, USA.
Shi
Bin
B
Sepp-Lorenzino
Laura
L
Baserga
Renato
R
D020413:3' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D045744:Cell Line, Tumor; D002999:Clone Cells; D017124:Conserved Sequence / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D020413:3' Untranslated Regions; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D012322:RNA Precursors