title: ICAM-1 mediates surface contact between neutrophils and keratocytes following corneal epithelial abrasion in the mouse.
abstract: Corneal epithelial abrasion elicits an inflammatory response involving neutrophil (PMN) recruitment from the limbal vessels into the corneal stroma. These migrating PMNs make surface contact with collagen and stromal keratocytes. Using mice deficient in PMN integrin CD18, we previously showed that PMN contact with stromal keratocytes is CD18-dependent, while contact with collagen is CD18-independent. In the present study, we wished to extend these observations and determine if ICAM-1, a known ligand for CD18, mediates PMN contact with keratocytes during corneal wound healing. Uninjured and injured right corneas from C57Bl/6 wild type (WT) mice and ICAM-1(-/-) mice were processed for transmission electron microscopy and imaged for morphometric analysis. PMN migration, stromal thickness, and ICAM-1 staining were evaluated using light microscopy. Twelve hours after epithelial abrasion, PMN surface contact with paralimbal keratocytes in ICAM-1(-/-) corneas was reduced to  ˜ 50% of that observed in WT corneas; PMN surface contact with collagen was not affected. Stromal thickness (edema), keratocyte network surface area and keratocyte shape were similar in ICAM-1(-/-) and WT corneas. WT keratocyte ICAM-1 expression was detected at baseline and ICAM-1 staining intensity increased following injury. Since ICAM-1 is readily detected on mouse keratocytes and PMN-keratocyte surface contact in ICAM-1(-/-) mice is markedly reduced, the data suggest PMN adhesive interactions with keratocyte-stromal networks is in part regulated by keratocyte ICAM-1 expression.
authors: College of Optometry, University of Houston, Houston, TX, USA. dgagen.2010@alumni.opt.uh.edu
authors: Debjani
authors: D
authors: Gagen
authors: Sara
authors: S
authors: Laubinger
authors: Zhijie
authors: Z
authors: Li
authors: Matei S
authors: MS
authors: Petrescu
authors: Evelyn S
authors: ES
authors: Brown
authors: C Wayne
authors: CW
authors: Smith
authors: Alan R
authors: AR
authors: Burns
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D003319:Corneal Stroma / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D019573:Epithelium, Corneal / Q000293:injuries*; D005131:Eye Injuries / Q000378:metabolism* / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D010641:Phenotype; D014945:Wound Healing / Q000502:physiology*; D014949:Wounds, Nonpenetrating / Q000378:metabolism* / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1