title: A mouse model for sleeve gastrectomy: applications for diabetes research.
abstract: BACKGROUND
  Discovery of enhanced glucose tolerance following bariatric surgery has sparked renewed interest in the investigation of unchartered underlying pathways of glucose homeostasis. Delineation of this pathway may ultimately be the first step in the creation of a novel therapy for type II diabetes. Nevertheless, the technical complexity and formidable nature of these surgeries coupled with the fragile nature of small rodents has made the creation of a mouse model to study these effects incredibly challenging. We have created a simplified sleeve gastrectomy mouse model to study the effects of bariatric surgery on glucose tolerance and beta cell proliferation.
  
  
  METHODS
  Nineteen mice were randomized to undergo either sleeve gastrectomy (SG) (9) or sham operation (SH) (10). Weight and serum glucose were measured three times weekly and serum insulin measurements and pancreatic harvest were performed at the time of sacrifice. Five mice from each group were sacrificed after one week and the remainder sacrificed after one month.
  
  
  RESULTS
  Survival of mice was 100% for both groups. The SG group demonstrated an initial drop in weight and serum glucose as compared to SH, which normalized by one month following surgery. Serum insulin levels and rate of beta cell proliferation were similar in both groups after one week and one month.
  
  
  CONCLUSIONS
  The simplified sleeve gastrectomy is a technically straightforward, low-mortality technique for creating a bariatric mouse model which most faithfully replicates bariatric surgery performed in humans. This model can be a valuable tool to investigate the glucose tolerance and beta cell effects of bariatric surgery.
authors: Department of Surgery, New York University Medical Center, NY, USA. avi.schlager@gmail.com
authors: Avraham
authors: A
authors: Schlager
authors: Abed
authors: A
authors: Khalaileh
authors: Yoav
authors: Y
authors: Mintz
authors: Mahmoud
authors: M
authors: Abu Gazala
authors: Anat
authors: A
authors: Globerman
authors: Nadav
authors: N
authors: Ilani
authors: Avraham I
authors: AI
authors: Rivkind
authors: Seth
authors: S
authors: Salpeter
authors: Yuval
authors: Y
authors: Dor
authors: Gideon
authors: G
authors: Zamir
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D049109:Cell Proliferation; D003920:Diabetes Mellitus / Q000378:metabolism; D005743:Gastrectomy / Q000379:methods*; D006706:Homeostasis / Q000502:physiology; D007150:Immunohistochemistry; D050417:Insulin-Secreting Cells / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism / Q000473:pathology; D051379:Mice; D023421:Models, Animal; D015431:Weight Loss / Q000502:physiology
chemical_list: D001786:Blood Glucose