Microwave-assisted extraction of human hair proteins.
The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25 mM Tris-HCl (pH 8.5), 2.6 M thiourea, 5 M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600 W) for a specified incubation period (5-120 min). The extraction efficiency of samples that had been incubated for 60 min was similar to that of samples that had been heated at 50°C for 24 h using the conventional Shindai method.
Shin
Seongjae
S
Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
Lee
Aera
A
Lee
Sunyoung
S
Lee
KyungJun
K
Kwon
Jongeun
J
Yoon
Mi Young
MY
Hong
Jangmi
J
Lee
Dongwook
D
Lee
Gae-Ho
GH
Kim
Jeongkwon
J
D006197:Hair / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000737:chemistry / Q000302:isolation & purification; D008872:Microwaves; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
D011506:Proteins; D007633:Keratins