title: Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain.
abstract: Morphogenesis of the mammalian forebrain is influenced by the patterning activity of signals emanating from the anterior mesendoderm. In this study, we show that procollagen IIA (IIA), an isoform of the cartilage extracellular matrix protein encoded by an alternatively spliced transcript of Col2a1, is expressed in the prechordal plate and the anterior definitive endoderm. In the absence of IIA activity, the null mutants displayed a partially penetrant phenotype of loss of head tissues, holoprosencephaly, and loss of mid-facial structures, which is associated with reduced sonic hedgehog (Shh) expression in the prechordal mesoderm. Genetic interaction studies reveal that IIA function in forebrain and face development does not involve bone morphogenetic protein receptor 1A (BMPR1A)- or NODAL-mediated signaling activity.
authors: Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors: Alan W L
authors: AW
authors: Leung
authors: Sandra Y Y
authors: SY
authors: Wong
authors: Danny
authors: D
authors: Chan
authors: Patrick P L
authors: PP
authors: Tam
authors: Kathryn S E
authors: KS
authors: Cheah
mesh_terms: D000818:Animals; D019521:Body Patterning; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics / Q000378:metabolism; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism*; D004707:Endoderm / Q000378:metabolism*; D006257:Head / Q000002:abnormalities; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D055457:Nodal Protein / Q000378:metabolism; D011347:Procollagen / Q000235:genetics / Q000378:metabolism*; D016548:Prosencephalon / Q000033:anatomy & histology / Q000196:embryology*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: C447413:Col2a1 protein, mouse; D024043:Collagen Type II; D053823:Hedgehog Proteins; D055457:Nodal Protein; D011347:Procollagen; D020033:Protein Isoforms; D012333:RNA, Messenger; C465570:Shh protein, mouse; C498212:Bmpr1a protein, mouse; D052005:Bone Morphogenetic Protein Receptors, Type I