Diurnal variation in LH and temporal relationships between oscillations in LH and progesterone during the luteal phase in heifers.
Diurnal variation in progesterone and LH during the luteal phase and the temporal relationships between oscillations of the two hormones were studied in 10 heifers by collection of blood samples at 0100, 0700, 1300, and 1900 h each day, beginning on Day 1 (Day 0 = ovulation). Concentration of LH on Days 5-9, but not on Days 10-14, was lower (P < 0.05) at 0700 h (0.25 ± 0.02 ng/mL) than at each of the other three hours (combined, 0.32 ± 0.02 ng/mL). An oscillation was defined as an uninterrupted increase and decrease in concentrations. The number of LH oscillations/heifer with the peak at 1900 h (6.1 ± 0.7) throughout the luteal phase was greater (P < 0.01) than for each of the other hours (combined, 4.0 ± 0.2). Diurnal variation in progesterone was not detected. Only statistically defined LH oscillations were used to determine the temporal association between the peak of an LH oscillation and various components of a progesterone oscillation. On Days 5-14, the frequency of the peak of an LH oscillation occurring at the same hour as the peak of a progesterone oscillation (26/48, 54%) was greater (P < 0.0001) than at the progesterone nadir (3/48, 6%). The frequency of the LH peak occurring during increasing (11/34, 32%) and decreasing (8/25, 32%) progesterone concentrations was intermediate (P < 0.05). Results indicated the following: 1) diurnal variation occurred in LH as determined by concentration and by the hour of the peak of an oscillation; and 2) LH oscillations were temporally and positively related to progesterone oscillations.
Hannan
M A
MA
Eutheria Foundation, Cross Plains, Wisconsin 53528, USA.
Fuenzalida
M J
MJ
Siddiqui
M A R
MA
Shamsuddin
M
M
Beg
M A
MA
Ginther
O J
OJ
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002940:Circadian Rhythm; D005260:Female; D008183:Luteal Phase / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood*; D011374:Progesterone / Q000097:blood*
D011374:Progesterone; D007986:Luteinizing Hormone