title: Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (alpha-Al2O3) and titanium (Ti-6Al-4V) alloy substrates.
abstract: Previous reports have shown the use of hydroxyapatite (HAp) and related calcium phosphate coatings on metal and nonmetal substrates for preparing tissue-engineering scaffolds, especially for osteogenic differentiation. These studies have revealed that the structural properties of coated substrates are dependent significantly on the method and conditions used for coating and also whether the substrates had been modified prior to the coating. In this article, we have done a comparative evaluation of the structural features of the HAp coatings, prepared by using simulated body fluid (SBF) at 25 degrees C for various time periods, on a nonporous metal substrate titanium-aluminium-vanadium (Ti-6Al-4V) alloy and a bioinert ceramic substrate alpha-alumina (alpha-Al(2)O(3)), with and without their prior treatment with the globular protein bovine serum albumin (BSA). Our analysis of these substrates by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectrometry showed significant and consistent differences in the quantitative and qualitative properties of the coatings. Interestingly, the bioactivity of these substrates in terms of supporting in vitro cell adhesion and spreading, and in vivo effects of implanted substrates, showed a predictable pattern, thus indicating that some coated substrates prepared under our conditions could be more suitable for biological/biomedical applications.
authors: Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India.
authors: Renu
authors: R
authors: Kapoor
authors: Pavana Goury
authors: PG
authors: Sistla
authors: Jerald Mahesh
authors: JM
authors: Kumar
authors: T Avinash
authors: TA
authors: Raj
authors: G
authors: G
authors: Srinivas
authors: Jui
authors: J
authors: Chakraborty
authors: Mithlesh K
authors: MK
authors: Sinha
authors: Debabrata
authors: D
authors: Basu
authors: Gopal
authors: G
authors: Pande
mesh_terms: D000497:Alloys; D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D008297:Male; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium; D000537:Aluminum Oxide