Synergistic and antagonistic interactions of phenolic compounds found in navel oranges.
Phenolic compounds are known to have antioxidant and antimicrobial properties. These properties may be useful in the preservation of foods or beverages. The interactive antioxidant capacity of phenolic compounds within foods has not been well explored. Interactions of individual phenolic compounds (chlorogenic acid, hesperidin, luteolin, myricetin, naringenin, p-coumaric acid, and quercetin) at the concentrations found in navel oranges (Citrus sinensis) were analyzed for their antioxidant capacity to observe potential antagonistic, additive, or synergistic interactions. Mixtures of 2, 3, and 4 phenolic compounds were prepared. The Oxygen Radical Absorbance Capacity (ORAC) assay was used to quantify the antioxidant capacities of these combinations. Three different combinations of 2 compounds and 5 combinations of 3 compounds were found to be synergistic. One antagonistic combination of 2 was also found. No additional synergism occurred with the addition of a 4th compound. A model was developed to explain our results. Reduction potentials, relative concentration, and the presence or absence of catechol (o-dihydroxy benzene) groups were factors in the model. Practical Application: Understanding how combinations of fruit antioxidants work together will support their future use in preservation of foods and/or beverages.
Freeman
Brenner L
BL
Dept. of Nutrition, Dietetics and Food Science, Brigham Young Univ., Provo, UT, USA.
Eggett
Dennis L
DL
Parker
Tory L
TL
D000975:Antioxidants / Q000737:chemistry*; D002934:Cinnamates / Q000737:chemistry*; D032084:Citrus sinensis / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006569:Hesperidin / Q000737:chemistry; D008956:Models, Chemical; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols
D000975:Antioxidants; D002934:Cinnamates; D044950:Flavanones; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D006569:Hesperidin; C005273:naringenin