[Identification and characterization of SP cells in human lung adenocarcinoma SPC-A1 cells.].
BACKGROUND
Recently, eloquent studies from some solid tumors have provided proofs that cancers originate from cancer stem cells (CSC). The discovery of CSC has changed our view of carcinogenesis and chemotherapy. The aim of this study is to identify and characterize the CSC population that drives and maintains lung adenocarcinoma growth and metastasis.


METHODS
Side population (SP) cell analysis combined with serum-free media (SFM) were applied to established human lung adenocarcinoma cell lines. Properties of SP cells were evaluated by their proliferative index, colony-forming efficiency and tumorigenic potential.


RESULTS
Characteristic SP cells could be detected by FACS in lung adenocarcinoma cell lines. And the proportion of SP cells is greatly increased after serum-free culture. SP cells have a greater proliferative index, a higher colony-forming efficiency and a greater ability to form tumor in vivo .


CONCLUSIONS
SP cells exist in human lung adenocarcinoma cell lines and they could be further enriched by preliminary serum-free culture before FACS sorting.
Zhu
Yanliang
Y
Department of Oncology, Nanjing General Hospital of Nanjing Military Command, Nanjing, Jiangsu 210002, China.
Chen
Longbang
L
Wang
Jinghua
J
Chu
Xiaoyuan
X
Chen
Yitian
Y
Zhang
Qun
Q
Xia
Xinyi
X