title: Permissive environment for B-cell maturation in myositis muscle in the absence of B-cell follicles.
abstract: Myositis muscle contains antigen-matured B-cells and plasma cells. Myositis muscle biopsy specimens were examined for nodular collections of T-cells, B-cells, myeloid dendritic cells, plasma cells, and follicular dendritic cells. Immunoglobulin and B-cell-activating factor (BAFF) transcripts were quantitated. Laser-capture microdissection was used to isolate single plasma cells, and their immunoglobulin transcripts were sequenced. Dense inflammatory infiltrates contained histological elements of ectopic lymphoid tissue but not B-cell follicles. Immunoglobulin transcript sequence analysis demonstrated spatially distributed, clonally related B-cells and plasma cells, suggesting local maturation of B-cells into plasma cells in myositis muscle. Regions of dense cellular infiltrates in myositis muscle are sometimes areas of B-cell maturation into antibody-producing plasma cells. An atypical lymphoid histology, lacking concentrated collections of germinal-center-like B-cell follicles, is capable of antigen-stimulated clonal maturation of antibody-producing plasma cells.
authors.affiliation: Department of Neurology, Division of Neuromuscular Disease, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, Massachusetts 02115, USA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salajegheh
authors.forename: Jack L
authors.initials: JL
authors.lastname: Pinkus
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Amato
authors.forename: Chris
authors.initials: C
authors.lastname: Morehouse
authors.forename: Bahija
authors.initials: B
authors.lastname: Jallal
authors.forename: Yihong
authors.initials: Y
authors.lastname: Yao
authors.forename: Steven A
authors.initials: SA
authors.lastname: Greenberg
mesh_terms: D017951:Antigen Presentation; D053264:B-Cell Activating Factor / Q000235:genetics; D053301:B-Cell Maturation Antigen / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016922:Cellular Senescence / Q000187:drug effects; D002828:Choristoma / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D018858:Germinal Center; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D066298:In Vitro Techniques; D008221:Lymphoid Tissue; D042282:Microdissection / Q000379:methods; D018482:Muscle, Skeletal / Q000473:pathology* / Q000503:physiopathology*; D009135:Muscular Diseases / Q000473:pathology; D009220:Myositis / Q000503:physiopathology*; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000473:pathology
chemical_list: D053264:B-Cell Activating Factor; D053301:B-Cell Maturation Antigen; D007136:Immunoglobulins; D012333:RNA, Messenger