title: Worldwide diversity of Klebsiella pneumoniae that produce beta-lactamase blaKPC-2 gene.
abstract: Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-AVB [18.8%]) and several acquired and plasmid-encoded genes (blaTEM-1 [81.3%], blaCTX-M-2 [31.3%], blaCTX-M-12 [12.5%], blaCTX-M-15 [18.7%], and blaOXA-9 [37.5%]). The blaKPC-2 gene was always associated with 1 of the Tn4401 isoforms (a, b, or c). Tn4401 was inserted on different-sized plasmids that belonged to different incompatibility groups. Several blaKPC-containing K. pneumoniae clones were found: 9 different pulsotypes with 1 major (sequence type 258) and 7 minor distinct allelic profiles. Different clones harboring different plasmids but having identical genetic structure, Tn4401, could be at the origin of the worldwide spread of this emerging resistance gene.
authors.affiliation: Institut National de la Santwe et de la Recherche Medicale, Paris, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Cuzon
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.forename: HaVy
authors.initials: H
authors.lastname: Truong
authors.forename: Maria Virginia
authors.initials: MV
authors.lastname: Villegas
authors.forename: Karin T
authors.initials: KT
authors.lastname: Wisell
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Carmeli
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gales
authors.forename: Shiri Navon
authors.initials: SN
authors.lastname: Venezia
authors.forename: John P
authors.initials: JP
authors.lastname: Quinn
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D014943:Global Health; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae