Prekallikrein deficiency in a dog.
Prekallikrein (PK) deficiency is an uncommon disorder in dogs. In this report, we describe a case of a dog that was referred for neurological defects and had a prolonged activated partial thromboplastin time (aPTT) and normal prothrombin time (PT) with no hemostatic defects. By using human PK-deficient plasma, the dog was diagnosed to have PK deficiency. The nucleotide sequence of normal canine PK cDNA was determined and compared with the genomic sequences of PK in the affected dog. The comparison revealed that the dog had a point mutation in exon 8 that leads to an amino acid substitution in the fourth apple domain of PK. This is the first report showing a point mutation of PK in a dog with PK deficiency.
Okawa
Takumi
T
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Yanase
Takuma
T
Shimokawa Miyama
Takako
T
Hiraoka
Hiroko
H
Baba
Kenji
K
Tani
Kenji
K
Okuda
Masaru
M
Mizuno
Takuya
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D020141:Hemostatic Disorders / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D010314:Partial Thromboplastin Time / Q000662:veterinary; D017354:Point Mutation; D011288:Prekallikrein / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D011288:Prekallikrein