The noncanonical Gag domains p8 and n are critical for assembly and release of mouse mammary tumor virus.
The mouse mammary tumor virus (MMTV) Gag contains the unique domains pp21, p3, p8, and n. We investigated the contribution of these domains to particle assembly and found that the region spanning the p8 and n domains is critical for shape determination and assembly. Deletion of pp21 and p3 reduced the number of released particles, but deletion of the n domain resulted in frequent formation of aberrant particles, while deletion of p8 severely impaired assembly. Further investigation of p8 revealed that both the basic and the proline-rich motifs within p8 contribute to MMTV assembly.
Zábranský
Ales
A
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n.2, 166 10 Prague 6, Czech Republic.
Hoboth
Peter
P
Hadravová
Romana
R
Stokrová
Jitka
J
Sakalian
Michael
M
Pichová
Iva
I
D000818:Animals; D036022:Capsid Proteins; D015683:Gene Products, gag / Q000502:physiology*; D008324:Mammary Tumor Virus, Mouse / Q000502:physiology*; D051379:Mice; D017434:Protein Structure, Tertiary; D019065:Virus Assembly
D036022:Capsid Proteins; D015683:Gene Products, gag