Unconventional layer-by-layer assembly of graphene multilayer films for enzyme-based glucose and maltose biosensing.
We have developed an unconventional method for the layer-by-layer (LbL) assembly of graphene multilayer films. Unconventional LbL assembly was achieved by the following two-step process. Graphene sheets were modified by pyrene-grafted poly(acrylic acid) (PAA) in aqueous solution, and then the modified graphene sheets were used for layer-by-layer alternating deposition with poly(ethyleneimine) (PEI). The graphene-multilayer-film-modified electrode shows enhanced electron transfer for the redox reactions of Fe(CN)(6)(3-) and excellent electrocatalytic activity of H(2)O(2). On the basis of this property, a bienzyme biosensing system for the detection of maltose was fabricated by successive LbL assembly of graphene, glucose oxidase (GOx), and glucoamylase (GA). LbL assembly of graphene combines the excellent electrochemical properties of graphene and the versatility of LbL assembly, showing great promise in highly efficient sensors and advanced biosensing systems.
Zeng
Guanghong
G
Key Lab of Organic Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing 100084, People's Republic of China.
Xing
Yibo
Y
Gao
Jian
J
Wang
Zhiqiang
Z
Zhang
Xi
X
D000180:Acrylic Resins / Q000737:chemistry; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004579:Electron Transport; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D008320:Maltose / Q000032:analysis*; D010087:Oxides / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D010087:Oxides; D014867:Water; C006903:carbopol 940; D008320:Maltose; D006108:Graphite; D011094:Polyethyleneimine; D005949:Glucose Oxidase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose