title: Characterization of a late expression gene of Bombyx mori nucleopolyhedrovirus.
abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) ORF5 (Bm5) is a gene present in many lepidopteran nucleopolyhedroviruses (NPVs), but its function is unknown. In this study, Bm5 was characterized. The transcript of Bm5 was detected 12-72 h post infection (p.i.). Polyclonal antiserum raised to a His-BM5 fusion protein recognized BM5 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm5 is a late gene. Immunofluorescence analysis by confocal microscopy showed that the BM5 protein is localized primarily in the cytoplasm. Localization of BM5 in budded virion (BV) and occlusion-derived virion (ODV) by Western analyses demonstrated that BM5 is not a structural protein associated with BV or ODV.
authors.affiliation: Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Hongxing
authors.initials: H
authors.lastname: Shen
authors.forename: Guiting
authors.initials: G
authors.lastname: Liang
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Yijia
authors.initials: Y
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology*; D016384:Consensus Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins