title: In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio.
abstract: The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg-Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Babaryka
authors.forename: E
authors.initials: E
authors.lastname: Kühn
authors.forename: R W
authors.initials: RW
authors.lastname: Köster
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000235:genetics; D018014:Gene Transfer Techniques; D012333:RNA, Messenger / Q000235:genetics; D019076:Transgenes; D015027:Zebrafish / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D015252:Deoxyribonucleases, Type II Site-Specific