Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine.
The dual native chemical ligation at lysine strategy was revised by replacing the acid-labile Cbz protecting group with photolabile NVOC at the 4-mercaptolysine side chain. The optimized strategy was subsequently applied to the synthesis of K48-linked diubiquitin.
Yang
Renliang
R
Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Pasunooti
Kalyan Kumar
KK
Li
Fupeng
F
Liu
Xue-Wei
XW
Liu
Chuan-Fa
CF
D001547:Benzaldehydes / Q000138:chemical synthesis / Q000737:chemistry; D008239:Lysine / Q000138:chemical synthesis / Q000737:chemistry*; D014452:Ubiquitins / Q000138:chemical synthesis* / Q000737:chemistry
D001547:Benzaldehydes; C402308:NVOC-chloride; D014452:Ubiquitins; D008239:Lysine