title: Factors affecting mark quality of alizarin complexone-stained otoliths in juvenile black bream Acanthopagrus butcheri and a prescription for dosage.
abstract: The relationships between otolith score, a function of increasing stain visibility, and the ratio of alizarin complexone (mg) to fish biomass (g) for 0.1, 0.6 and 7 g black bream Acanthopagrus butcheri show that acceptable marks are produced in all size classes using a stain-to-biomass ratio of 3.3 mg g(-1) following an immersion period of 24 h.
authors.affiliation: Aquaculture Development Unit, WA Maritime Training Centre, 1 Fleet Street, Fremantle, Western Australia 6160, Australia. gavin.partridge@challengertafe.wa.edu.au
authors.forename: G J
authors.initials: GJ
authors.lastname: Partridge
authors.forename: G I
authors.initials: GI
authors.lastname: Jenkins
authors.forename: R G
authors.initials: RG
authors.lastname: Doupé
authors.forename: S
authors.initials: S
authors.lastname: De Lestang
authors.forename: B M
authors.initials: BM
authors.lastname: Ginbey
authors.forename: D
authors.initials: D
authors.lastname: French
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D005398:Fisheries / Q000379:methods*; D010037:Otolithic Membrane / Q000737:chemistry*; D010473:Perciformes / Q000502:physiology*; D011156:Population Density; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000880:Anthraquinones; C070804:alizarin complexone