In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio.
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
Babaryka
A
A
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Ingolstädter Landstrasse 1, 85764 Neuherberg-Munich, Germany.
Kühn
E
E
Köster
R W
RW
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000235:genetics; D018014:Gene Transfer Techniques; D012333:RNA, Messenger / Q000235:genetics; D019076:Transgenes; D015027:Zebrafish / Q000235:genetics*
D012333:RNA, Messenger; D015252:Deoxyribonucleases, Type II Site-Specific