Cancer-preventive activities of secondary metabolites from leaves of the bilberry Vaccinium smallii A. Gray.
Ten secondary metabolites including flavonoids (1-8), caffeic (9) and chlorogenic (10) acids were structurally identified from the extract of Sakhalin bilberry Vaccinium smallii leaves and studied in vitro as potential cancer-preventive agents. The results showed that compounds 1-10 inhibited EGF-induced neoplastic transformation of mouse JB6 Cl 41 P+ cells in soft agar with an inhibition concentration (INCC50) of 20-80 µm. Moreover, all these natural products were non-toxic against JB6 Cl 41 P+ cells up to a concentration of 200 µm.
Mechikova
G Ya
GY
Far-Eastern State Medical University, Murav'eva-Amurskogo st. 35, Khabarovsk, 680000, Russian Federation.
Kuzmich
A S
AS
Ponomarenko
L P
LP
Kalinovsky
A I
AI
Stepanova
T A
TA
Fedorov
S N
SN
Stonik
V A
VA
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002109:Caffeic Acids / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002726:Chlorogenic Acid / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D029800:Vaccinium myrtillus / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D002109:Caffeic Acids; D005419:Flavonoids; D010936:Plant Extracts; D002726:Chlorogenic Acid; C040048:caffeic acid