Optimization and purification of L-asparaginase produced by Streptomyces tendae TK-VL_333.
Cultural factors affecting the production of L-asparaginase by Streptomyces tendae isolated from laterite soil samples of Guntur region were investigated on glycerol-asparagine-salts (modified ISP-5) broth. Optimal yields of L-asparaginase were recorded in the culture medium with the initial pH 7.0 incubated at 30 degrees C for 72 h. The strain utilized sucrose (2%) and yeast (2%) extract as carbon and nitrogen sources for L-asparaginase production. The productivity of L-asparaginase was slightly enhanced when the strain was treated with cell-disrupting agents like EDTA. The crude enzyme was purified to homogeneity by ammonium sulfate precipitation, Sephadex G-100 and CM-Sephadex G-50 gel filtration. By employing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight of the enzyme was recorded as 97.4 kDa. This is the first report on production and purification of L-asparaginase from S. tendae.
Kavitha
Alapati
A
Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur - 522 510, Andhra Pradesh, India.
Vijayalakshmi
Muvva
M
D001215:Asparaginase / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003911:Dextrans / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007194:India; D008970:Molecular Weight; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification
D003911:Dextrans; D001215:Asparaginase