A reverse genetic approach to test functional redundancy during embryogenesis.
Gene function during embryogenesis is typically defined by loss-of-function experiments, for example by targeted mutagenesis (knockout) in the mouse. In the zebrafish model, effective reverse genetic techniques have been developed using microinjection of gene-specific antisense morpholinos. Morpholinos target an mRNA through specific base-pairing and block gene function transiently by inhibiting translation or splicing for several days during embryogenesis (knockdown). However, in vertebrates such as mouse or zebrafish, some gene functions can be obscured by these approaches due to the presence of another gene that compensates for the loss. This is especially true for gene families containing sister genes that are co-expressed in the same developing tissues. In zebrafish, functional compensation can be tested in a relatively high-throughput manner, by co-injection of morpholinos that target knockdown of both genes simultaneously. Likewise, using morpholinos, a genetic interaction between any two genes can be demonstrated by knockdown of both genes together at sub-threshold levels. For example, morpholinos can be titrated such that neither individual knockdown generates a phenotype. If, under these conditions, co-injection of both morpholinos causes a phenotype, a genetic interaction is shown. Here we demonstrate how to show functional redundancy in the context of two related GATA transcription factors. GATA factors are essential for specification of cardiac progenitors, but this is revealed only by the loss of both Gata5 and Gata6. We show how to carry out microinjection experiments, validate the morpholinos, and evaluate the compensated phenotype for cardiogenesis.
Rikin
Amir
A
Department of Surgery, Weill Cornell Medical College of Cornell University, USA.
Rosenfeld
Gabriel E
GE
McCartin
Kellie
K
Evans
Todd
T
D000818:Animals; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D050980:GATA Transcription Factors / Q000235:genetics; D050992:GATA5 Transcription Factor / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods*; D008297:Male; D016376:Oligonucleotides, Antisense / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
D050980:GATA Transcription Factors; D050992:GATA5 Transcription Factor; C525957:Gata4 protein, zebrafish; D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins; C494754:gata5 protein, zebrafish