title: Interaction of PqqE and PqqD in the pyrroloquinoline quinone (PQQ) biosynthetic pathway links PqqD to the radical SAM superfamily.
abstract: pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD in PQQ biosynthesis.
authors.affiliation: Department of Chemistry, Department of Molecular and Cell Biology, and the California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Wecksler
authors.forename: Stefan
authors.initials: S
authors.lastname: Stoll
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Iavarone
authors.forename: Erin M
authors.initials: EM
authors.lastname: Imsand
authors.forename: Ha
authors.initials: H
authors.lastname: Tran
authors.forename: R David
authors.initials: RD
authors.lastname: Britt
authors.forename: Judith P
authors.initials: JP
authors.lastname: Klinman
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology; D045542:PQQ Cofactor / Q000096:biosynthesis* / Q000737:chemistry; D012436:S-Adenosylmethionine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005609:Free Radicals; D045542:PQQ Cofactor; D012436:S-Adenosylmethionine