Worldwide diversity of Klebsiella pneumoniae that produce beta-lactamase blaKPC-2 gene.
Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-AVB [18.8%]) and several acquired and plasmid-encoded genes (blaTEM-1 [81.3%], blaCTX-M-2 [31.3%], blaCTX-M-12 [12.5%], blaCTX-M-15 [18.7%], and blaOXA-9 [37.5%]). The blaKPC-2 gene was always associated with 1 of the Tn4401 isoforms (a, b, or c). Tn4401 was inserted on different-sized plasmids that belonged to different incompatibility groups. Several blaKPC-containing K. pneumoniae clones were found: 9 different pulsotypes with 1 major (sequence type 258) and 7 minor distinct allelic profiles. Different clones harboring different plasmids but having identical genetic structure, Tn4401, could be at the origin of the worldwide spread of this emerging resistance gene.
Cuzon
Gaelle
G
Institut National de la Santwe et de la Recherche Medicale, Paris, France.
Naas
Thierry
T
Truong
HaVy
H
Villegas
Maria Virginia
MV
Wisell
Karin T
KT
Carmeli
Yehuda
Y
Gales
Ana C
AC
Venezia
Shiri Navon
SN
Quinn
John P
JP
Nordmann
Patrice
P
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D014943:Global Health; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae