title: Resistance to tobramycin and colistin in isolates of Pseudomonas aeruginosa from chronically colonized patients with cystic fibrosis under antimicrobial treatment.
abstract: Tobramycin and colistin represent 2 standard antimicrobial agents in the treatment of cystic fibrosis (CF) patients who are chronically colonized with Pseudomonas aeruginosa. In this study, we determined the rate of resistance to tobramycin and colistin in 1844 isolates of P. aeruginosa obtained from 22 CF patients under alternate therapy with inhaled tobramycin and colistin. Resistance to tobramycin was observed in 27.5% of isolates. In contrast, all isolates were susceptible to colistin. Molecular typing of selected isolates suggested that only 1 clone occurred over time in each patient. To conclude, resistance to tobramycin in P. aeruginosa isolates from CF patients under antimicrobial therapy may occur while colistin resistance remains uncommon.
authors.affiliation: Institute of Hygiene and Microbiology, University of Würzburg, Würzburg, Germany. gvalenza@hygiene.uni-wuerzburg.de
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valenza
authors.forename: Kerstin
authors.initials: K
authors.lastname: Radike
authors.forename: Christoph
authors.initials: C
authors.lastname: Schoen
authors.forename: Stephan
authors.initials: S
authors.lastname: Horn
authors.forename: Anett
authors.initials: A
authors.lastname: Oesterlein
authors.forename: Matthias
authors.initials: M
authors.lastname: Frosch
authors.forename: Marianne
authors.initials: M
authors.lastname: Abele-Horn
authors.forename: Helge
authors.initials: H
authors.lastname: Hebestreit
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D002908:Chronic Disease; D016000:Cluster Analysis; D003091:Colistin / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000150:complications* / Q000188:drug therapy; D016172:DNA Fingerprinting; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D014031:Tobramycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D014031:Tobramycin; D003091:Colistin