Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica.
The presence of sulphur in fossil fuels and the natural environment justifies the study of sulphur-utilising bacterial species and genes involved in the biodesulphurisation process. Technology has been developed based on the natural ability of microorganisms to remove sulphur from polycyclic aromatic hydrocarbon chains. This biotechnology aims to minimise the emission of sulphur oxides into the atmosphere during combustion and prevent the formation of acid rain. In this study, the isolation and characterization of desulphurising microorganisms in rhizosphere and bulk soil samples from Antarctica that were either contaminated with oil or uncontaminated was described. The growth of selected isolates and their capacity to utilise sulphur based on the formation of the terminal product of desulphurisation via the 4S pathway, 2-hydroxybiphenyl, was analysed. DNA was extracted from the isolates and BOX-PCR and DNA sequencing were performed to obtain a genomic diversity profile of cultivable desulphurising bacterial species. Fifty isolates were obtained showing the ability of utilising dibenzothiophene as a substrate and sulphur source for maintenance and growth when plated on selective media. However, only seven genetically diverse isolates tested positive for sulphur removal using the Gibbs assay. DNA sequencing revealed that these isolates were related to the genera Acinetobacter and Pseudomonas.
Boniek
Douglas
D
Graduate Program in Environmental Engineering, Institute of Biological and Exact Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil.
Figueiredo
Débora
D
Pylro
Victor Satler
VS
Duarte
Gabriela Frois
GF
D000222:Adaptation, Physiological; D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000378:metabolism; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000302:isolation & purification; D005579:Fossil Fuels; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D007700:Kinetics; D010641:Phenotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D005579:Fossil Fuels; D012989:Soil Pollutants; D013876:Thiophenes; D014874:Water Pollutants, Chemical; D013455:Sulfur; C004369:2-phenylphenol; C016366:dibenzothiophene