Oxidative stress, systemic inflammation, and severe periodontitis.
Periodontal infections have been associated with a state of chronic inflammation. To ascertain whether severe periodontitis and its treatment are associated with oxidative stress, we recruited 145 cases (periodontitis) and 56 controls in a case-control study. A further pilot intervention study of 14 cases (periodontal therapy) was performed. Blood samples were taken at baseline (case-control) and 1, 3, 5, 7, and 30 days after treatment (intervention). Diacron-reactive oxygen metabolites (D-ROM), anti-oxidant potential, C-reactive protein (CRP), interleukin-6, and lipid profiles were determined with high-sensitivity assays in serum. Patients with severe periodontitis exhibited higher D-ROM levels (P < 0.001) and lower total anti-oxidant capacity (P < 0.001) compared with healthy control individuals. These findings were independent of age, gender, smoking habits, ethnicity, and standard lipids differences. D-ROM levels were positively correlated with CRP (R = 0.4, P < 0.001) and clinical periodontal parameters (R = 0.20, P < 0.05). Acute increases of D-ROM (P < 0.01) were observed following periodontal therapy. Analysis of these data suggests a positive association between severe periodontitis and oxidative stress.
D'Aiuto
F
F
Periodontology Unit, Division of Clinical Research, UCL Eastman Dental Institute and Hospital, 256 Gray's Inn Road, London WC1X 8LD, UK. f.daiuto@eastman.ucl.ac.uk
Nibali
L
L
Parkar
M
M
Patel
K
K
Suvan
J
J
Donos
N
N
D000367:Age Factors; D010520:Aggressive Periodontitis / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D016301:Alveolar Bone Loss / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D000975:Antioxidants / Q000032:analysis; D002097:C-Reactive Protein / Q000032:analysis; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D005006:Ethnicity; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000503:physiopathology*; D015850:Interleukin-6 / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010514:Periodontal Pocket / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D010518:Periodontitis / Q000097:blood / Q000503:physiopathology* / Q000628:therapy; D010865:Pilot Projects; D017382:Reactive Oxygen Species / Q000097:blood; D012737:Sex Factors; D012907:Smoking
D000975:Antioxidants; D015850:Interleukin-6; D008055:Lipids; D017382:Reactive Oxygen Species; D002097:C-Reactive Protein