PCR-RFLP analysis of mitochondrial DNA in tench Tinca tinca.
Polymorphism was detected at ND1, ND6, D-loop and cyt b segments of mtDNA in 105 tench (Tinca tinca L.), using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and five composite haplotypes were identified. The diversity indices and the results of the population comparisons revealed that the identified markers provide a powerful tool for further studies on this species.
Lo Presti
R
R
Dipartimento di Scienze Zootecniche, Università di Torino, Via Leonardo da Vinci 44, 10095 Grugliasco, Italy. rossella.lopresti@unito.it
Gasco
L
L
Lisa
C
C
Zoccarato
I
I
Di Stasio
L
L
D000818:Animals; D003530:Cyprinidae / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D007558:Italy; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D045483:Rivers
D004272:DNA, Mitochondrial; D029941:Fish Proteins; D005819:Genetic Markers