title: Application of a sensitive, specific and controlled real-time PCR assay to surveillance indicates a low prevalence of viral haemorrhagic septicaemia virus (VHSV) in wild herring, Clupea harengus L., in Scottish waters.
abstract: Surveillance data on the distribution of viral haemorrhagic septicaemia virus (VHSV) in the North Sea (UK), targeting Atlantic herring in areas with previous virus detection, were obtained from research cruises conducted during 2005. The sensitive molecular approach of real-time RT-PCR (qRT-PCR) was applied alongside a newly developed endogenous positive control assay specific for herring (elongation factor 1α) to ensure integrity of template. Three hundred and five pools from 1937 individual herring were tested, and no evidence of VHSV in association with wild Atlantic herring was detected. Samples were obtained from Scottish waters where marine aquaculture is conducted. The results confirm that previous tissue culture studies have most likely not significantly underestimated the prevalence of carrier herring in this area. The significance of migratory species such as herring as a reservoir species for VHSV, with the potential to translocate virus genotypes between geographical areas, is discussed.
authors.affiliation: Marine Scotland, Marine Laboratory, Aberdeen, UK. I.Matejusova@marlab.ac.uk
authors.forename: I
authors.initials: I
authors.lastname: Matejusova
authors.forename: P
authors.initials: P
authors.lastname: McKay
authors.forename: F
authors.initials: F
authors.lastname: Bland
authors.forename: M
authors.initials: M
authors.lastname: Snow
mesh_terms: D025041:Animal Migration / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005399:Fishes; D005843:Geography; D031941:Hemorrhagic Septicemia, Viral / Q000453:epidemiology* / Q000821:virology*; D008969:Molecular Sequence Data; D009659:North Sea; D029141:Novirhabdovirus / Q000235:genetics*; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012606:Scotland / Q000453:epidemiology; D012680:Sensitivity and Specificity; D018571:Sentinel Surveillance / Q000662:veterinary*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D020648:Peptide Elongation Factor 1