Structure and RNA interactions of the plant MicroRNA processing-associated protein HYL1.
HYL1 is a double-stranded RNA binding protein involved in microRNA processing in plants. HYL1 enhances the efficiency and precision of the RNase III protein DCL1 and participates in microRNA strand selection. In this work, we dissect the contributions of the domains of HYL1 to the binding of RNA targets. We found that the first domain is the main contributor to RNA binding. Mapping of the interaction regions by nuclear magnetic resonance on the structure of HYL1 RNA-binding domains showed that the difference in binding capabilities can be traced to sequence divergence in β2-β3 loop. The possible role of each domain is discussed in light of previous experimental data.
Rasia
Rodolfo M
RM
Instituto de Biología Molecular y Celular de Rosario, División Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina. rasia@ibr.gov.ar
Mateos
Julieta
J
Bologna
Nicolás G
NG
Burdisso
Paula
P
Imbert
Lionel
L
Palatnik
Javier F
JF
Boisbouvier
Jerome
J
D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D018797:Cell Cycle Proteins; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012313:RNA / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D043244:Ribonuclease III
D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; C420966:HYL1 protein, Arabidopsis; D035683:MicroRNAs; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D012313:RNA; C402383:DCL1 protein, Arabidopsis; D043244:Ribonuclease III