Characterization of a late expression gene of Bombyx mori nucleopolyhedrovirus.
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF5 (Bm5) is a gene present in many lepidopteran nucleopolyhedroviruses (NPVs), but its function is unknown. In this study, Bm5 was characterized. The transcript of Bm5 was detected 12-72 h post infection (p.i.). Polyclonal antiserum raised to a His-BM5 fusion protein recognized BM5 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm5 is a late gene. Immunofluorescence analysis by confocal microscopy showed that the BM5 protein is localized primarily in the cytoplasm. Localization of BM5 in budded virion (BV) and occlusion-derived virion (ODV) by Western analyses demonstrated that BM5 is not a structural protein associated with BV or ODV.
Zhou
Yang
Y
Institute of Life Sciences, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, PR China.
Chen
Keping
K
Yao
Qin
Q
Shen
Hongxing
H
Liang
Guiting
G
Li
Xiaogang
X
Wang
Nan
N
Li
Yijia
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology*; D016384:Consensus Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins