Label-free detection of DNA hybridization using surface enhanced Raman spectroscopy.
The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm(-1); the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.
Barhoumi
Aoune
A
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
Halas
Naomi J
NJ
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D009838:Oligodeoxyribonucleotides; D004247:DNA