Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection.
Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pK(a) determination of small molecule analytes is demonstrated with the multilane microchip.
Nikcevic
Irena
I
Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA.
Piruska
Aigars
A
Wehmeyer
Kenneth R
KR
Seliskar
Carl J
CJ
Limbach
Patrick A
PA
Heineman
William R
WR
D001578:Benzopyrans; D002264:Carboxylic Acids; D019075:Electrophoresis, Capillary / Q000379:methods*; D019793:Fluorescein; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D046208:Microchip Analytical Procedures; D044085:Microfluidics; D009284:Naphthols; D012235:Rhodamines; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001578:Benzopyrans; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D009284:Naphthols; C436864:Oregon Green 488 carboxylic acid; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D019793:Fluorescein