title: Ultrasound ionization of biomolecules.
abstract: To date, mass spectrometric analysis of biomolecules has been primarily performed with either matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization (ESI). In this work, ultrasound produced by a simple piezoelectric device is shown as an alternative method for soft ionization of biomolecules. Precursor ions of proteins, saccharides and fatty acids showed little fragmentation. Cavitation is considered as a primary mechanism for the ionization of biomolecules.
authors.affiliation: The Genomics Research Center, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: Chen-I
authors.initials: CI
authors.lastname: Wu
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Nelson G
authors.initials: NG
authors.lastname: Chen
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Chen
mesh_terms: D000809:Angiotensins / Q000737:chemistry; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D019826:Cholic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry; D007477:Ions / Q000737:chemistry*; D016014:Linear Models; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000809:Angiotensins; D002241:Carbohydrates; D005227:Fatty Acids; D007328:Insulin; D007477:Ions; D011506:Proteins; D019826:Cholic Acid