The effect of a phytosphingosine-like substance isolated from Asterina pectinifera on involucrin expression in mite antigen-stimulated HaCaT cells.
The aim of the present study was to investigate the effect of phytosphingosine (PS) on mite antigen-induced terminal differentiation abnormalities in HaCaT cells. For this purpose, a PS-like substance was isolated from Asterina pectinifera (starfish PS) using high-performance liquid chromatography and was partially characterized through 1H NMR analysis. The level of involucrin expression in HaCaT cell was measured by immunoblotting assay. Our results showed that PS treatments remarkably up-regulated the involucrin expression, which is known as a terminal differentiation marker in the epidermal mite antigen-treated HaCaT cells. This indicates that starfish PS could regulate mite antigen-induced terminal differentiation fluctuation in the epidermis. Taken together, the results suggest that starfish PS might be a useful therapeutic agent for atopic dermatitis.
Choi
Gui Hyang
GH
School of Life Science and Biotechnology, College of Natural Science, Kyungpook National University, Daegu 702-701, Korea.
Wahid
Fazli
F
Kim
You Young
YY
D000818:Animals; D000941:Antigens / Q000276:immunology / Q000494:pharmacology*; D047288:Asterina / Q000737:chemistry*; D045744:Cell Line, Tumor; D008925:Mites / Q000276:immunology*; D011498:Protein Precursors / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D000941:Antigens; D011498:Protein Precursors; C032253:involucrin; C012491:phytosphingosine; D013110:Sphingosine