title: Microbiological findings of culture-positive preservation fluid in liver transplantation.
abstract: Bacterial and fungal infections are the leading cause of mortality in liver transplant (LT) recipients. Few studies have examined the incidence of culture-positive preservation fluid (PF) and the outcome of related recipients. The aim of this study was to determine the incidence and the microbiologic findings of PF positive cultures, and to evaluate the impact on morbidity and mortality of LT recipients. A retrospective analysis of PF cultures performed after 477 LTs from cadaveric grafts between January 2001 and February 2008 was conducted. Forty-five (9.5%) PFs were found to be positive with 1 or 2 pathogens. The demographic profiles of recipients of PF with positive or negative cultures were similar. Enterobacteriaceae species were the most frequent organisms (n = 30), followed by Staphylococcus aureus (n = 5), coagulase-negative staphylococci (n = 5), enterococci (n = 4), and yeasts (n = 3). Mortality rate at 1 month was not significantly different in recipients with positive or sterile PF cultures (88.1% vs. 87.7%, respectively). The rate of bacteremia among LT recipients with positive or negative PF cultures was not statistically different. Systemic infections caused by the pathogen cultured from the PF occurred in 8 (18%) of the 45 recipients, including bacteremia (4/8) or intra-abdominal sepsis (5/8). Causative organisms were Enterobacteriaceae species (n = 5), Candida species (n = 2), and Enterococcus faecium (n = 1). Among the 8 patients who developed infection with the PF organism, 4 (50%) died in the intensive care unit (ICU) vs. an ICU mortality rate of 8% (3/37) in those who did not develop infection with the PF organism (P < 0.05). Infection occurred less frequently in recipients who received antimicrobial therapy with activity against the PF isolate than in those without appropriate treatment (41% vs. 3.8%, P < 0.005). Those who develop infection with organisms recovered from PF cultures appear to have high early mortality rates; therefore, appropriate antimicrobial therapy against organisms cultured from PF should be given.
authors.affiliation: Department of Anesthesiology, Beaujon University Hospital, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris, Clichy, France. sylvie.janny@bjn.aphp.fr
authors.forename: S
authors.initials: S
authors.lastname: Janny
authors.forename: F
authors.initials: F
authors.lastname: Bert
authors.forename: F
authors.initials: F
authors.lastname: Dondero
authors.forename: F
authors.initials: F
authors.lastname: Durand
authors.forename: P
authors.initials: P
authors.lastname: Guerrini
authors.forename: P
authors.initials: P
authors.lastname: Merckx
authors.forename: M H
authors.initials: MH
authors.lastname: Nicolas-Chanoine
authors.forename: J
authors.initials: J
authors.lastname: Belghiti
authors.forename: J
authors.initials: J
authors.lastname: Mantz
authors.forename: C
authors.initials: C
authors.lastname: Paugam-Burtz
mesh_terms: D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000401:mortality; D003470:Culture Media; D004340:Drug Contamination; D005260:Female; D005658:Fungi / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006095:Gram-Positive Cocci / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D008099:Liver / Q000382:microbiology; D008107:Liver Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000401:mortality; D016031:Liver Transplantation / Q000009:adverse effects* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology / Q000401:mortality; D019188:Organ Preservation Solutions / Q000032:analysis*
chemical_list: D003470:Culture Media; D019188:Organ Preservation Solutions