title: Analysis and identification of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous by high-performance liquid chromatography.
abstract: This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and UV-vis absorption spectra. Peaks showed in the HPLC chromatogram are identified as carotenoids in the monocyclic biosynthetic pathway or their derivatives. In the monocyclic carotenoid pathway, 3,3'-dihydroxy-beta,psi-carotene-4,4'-dione (DCD) is produced through gamma-carotene and torulene.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Lu
authors.forename: Yang'e
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhao
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhou
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D002338:Carotenoids / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D013053:Spectrophotometry / Q000379:methods; D024341:Xanthophylls / Q000096:biosynthesis / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; C005948:astaxanthine