Retroviral expression of arginine decarboxylase attenuates oxidative burden in mouse cortical neural stem cells.
Neural stem cells (NSCs) have the potential to integrate seamlessly into the host tissues, and the development of potential stem cells resistant to stress injury is an elusive goal for efficient therapeutic application. Oxidative injury induces cellular and nuclear damages and the balanced regulation of reactive oxygen species is of critical significance for stem cell development, function, and survival. Agmatine, an endogenous primary amine and a novel neuromodulator synthesized from the decarboxylation of l-arginine catalyzed by arginine decarboxylase (ADC), has been reported to possess neuroprotective properties. In the present study, we determined whether the expression of ADC in NSCs can prevent the cells from oxidative injury. Retrovirus expressing human (ADC), (vhADC) was generated using a pLXSN vector. Cortical NSCs were infected with vhADC and subjected to H₂O₂ injury (200 μM for 15 h). Reverse transcriptase-polymerase chain reaction and immunocytochemical staining revealed that hADC mRNA and protein were highly expressed in the vhADC-infected NSCs (ADC-NSCs). High performance liquid chromatography (HPLC) analysis confirmed high concentration of agmatine in the ADC-NSCs, when exposed to H₂O₂ injury. Lactate dehydrogenase leakage and intracellular reactive oxygen species formation were about 2-fold reduced in ADC-NSCs when compared with control NSCs and NSCs infected with mock vector (P < 0.05). DNA fragmentation, chromatin condensation, and expression of apoptotic proteins such as p53, bax, and caspase-3 cleavage were significantly decreased in ADC-NSCs (P < 0.05), suggesting the prevention of apoptotic cell death following H₂O₂ injury. Our study demonstrates that overexpression of ADC is an effective novel approach to protect stem cells from oxidative damage.
Bokara
Kiran Kumar
KK
Department of Anatomy, Yonsei University College of Medicine, Seoul, South Korea.
Kwon
Ki Hyo
KH
Nho
Yoonmi
Y
Lee
Won Taek
WT
Park
Kyung Ah
KA
Lee
Jong Eun
JE
D000376:Agmatine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005822:Genetic Vectors; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D058953:Neural Stem Cells / Q000378:metabolism*; D018384:Oxidative Stress; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D012190:Retroviridae / Q000235:genetics
D051017:Apoptosis Regulatory Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D000376:Agmatine; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002262:Carboxy-Lyases; C019722:arginine decarboxylase