Analysis and identification of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous by high-performance liquid chromatography.
This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and UV-vis absorption spectra. Peaks showed in the HPLC chromatogram are identified as carotenoids in the monocyclic biosynthetic pathway or their derivatives. In the monocyclic carotenoid pathway, 3,3'-dihydroxy-beta,psi-carotene-4,4'-dione (DCD) is produced through gamma-carotene and torulene.
Lu
Mingbo
M
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Zhang
Yang'e
Y
Zhao
Chunfang
C
Zhou
Pengpeng
P
Yu
Longjiang
L
D002338:Carotenoids / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D013053:Spectrophotometry / Q000379:methods; D024341:Xanthophylls / Q000096:biosynthesis / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism
D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; C005948:astaxanthine