title: High throughput cell nanomechanics with mechanical imaging interferometry.
abstract: The dynamic nanomechanical properties of a large number of cells (up to hundreds), measured in parallel with high throughput, are reported. Using NIH 3T3 and HEK 293T fibroblasts and actin depolymerizing drugs, we use a novel nanotechnology to quantify the local viscoelastic properties with applied forces of 20 pN-20 nN, a spatial resolution of <20 nm, and a mechanical dynamic range of several Pa up to ~200 kPa. Our approach utilizes imaging interferometry in combination with reflective, magnetic probes attached to cells. These results indicate that mechanical imaging interferometry is a sensitive and scalable technology for measuring the nanomechanical properties of large arrays of live cells in fluid.
authors.affiliation: Department of Chemistry and Biochemistry, UCLA, 607 Charles Young Drive East, Los Angeles, CA 90095, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Reed
authors.forename: Matthew
authors.initials: M
authors.lastname: Frank
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Troke
authors.forename: Joanna
authors.initials: J
authors.lastname: Schmit
authors.forename: Sen
authors.initials: S
authors.lastname: Han
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: James K
authors.initials: JK
authors.lastname: Gimzewski