Efficiency of fine-needle aspiration compared with other sampling techniques for laboratory diagnosis of Buruli ulcer disease.
In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcerative lesions in cases where scarred edges prevent the collection of swabs.
Herbinger
Karl-Heinz
KH
Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University of Munich, Munich, Germany. herbinger@lrz.uni-muenchen.de
Beissner
Marcus
M
Huber
Kristina
K
Awua-Boateng
Nana-Yaa
NY
Nitschke
Jörg
J
Thompson
William
W
Klutse
Erasmus
E
Agbenorku
Pius
P
Assiobo
Awovi
A
Piten
Ebekalisai
E
Wiedemann
Franz
F
Fleischmann
Erna
E
Helfrich
Kerstin
K
Adjei
Ohene
O
Löscher
Thomas
T
Bretzel
Gisela
G
D001431:Bacteriological Techniques / Q000379:methods*; D044963:Biopsy, Fine-Needle / Q000379:methods*; D054312:Buruli Ulcer / Q000175:diagnosis; D006801:Humans; D008853:Microscopy / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*