title: Structural evidence of glycoprotein assembly in cellular membrane compartments prior to Alphavirus budding.
abstract: Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, CA 95616-8536, USA.
authors.forename: Pan
authors.initials: P
authors.lastname: Soonsawad
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.forename: Emerson
authors.initials: E
authors.lastname: Milla
authors.forename: Juan M
authors.initials: JM
authors.lastname: Espinoza
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kawano
authors.forename: Michael
authors.initials: M
authors.lastname: Marko
authors.forename: Chyongere
authors.initials: C
authors.lastname: Hsieh
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Furukawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Wattana
authors.initials: W
authors.lastname: Weerachatyanukul
authors.forename: Ranjana
authors.initials: R
authors.lastname: Srivastava
authors.forename: Susan W
authors.initials: SW
authors.lastname: Barnett
authors.forename: Indresh K
authors.initials: IK
authors.lastname: Srivastava
authors.forename: R Holland
authors.initials: RH
authors.lastname: Cheng
mesh_terms: D000524:Alphavirus / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology*; D006224:Cricetinae; D055032:Electron Microscope Tomography; D006023:Glycoproteins / Q000378:metabolism*; D014617:Vacuoles; D014764:Viral Proteins / Q000378:metabolism; D019065:Virus Assembly; D057074:Virus Release
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins