title: In vitro expansion of autonomously contracting, cardiomyogenic structures from rainbow trout Oncorhynchus mykiss.
abstract: In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss, the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presence of cardiomyocytes.
authors.affiliation: Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Paul Ehrlich Str. 1-3, 23562, Lübeck, Germany. bianka.grunow@emb.fraunhofer.de
authors.forename: B
authors.initials: B
authors.lastname: Grunow
authors.forename: P
authors.initials: P
authors.lastname: Ciba
authors.forename: S
authors.initials: S
authors.lastname: Rakers
authors.forename: M
authors.initials: M
authors.lastname: Klinger
authors.forename: E
authors.initials: E
authors.lastname: Anders
authors.forename: C
authors.initials: C
authors.lastname: Kruse
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D046529:Microscopy, Electron, Transmission; D009119:Muscle Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology