title: Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine.
abstract: The dual native chemical ligation at lysine strategy was revised by replacing the acid-labile Cbz protecting group with photolabile NVOC at the 4-mercaptolysine side chain. The optimized strategy was subsequently applied to the synthesis of K48-linked diubiquitin.
authors.affiliation: Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
authors.forename: Renliang
authors.initials: R
authors.lastname: Yang
authors.forename: Kalyan Kumar
authors.initials: KK
authors.lastname: Pasunooti
authors.forename: Fupeng
authors.initials: F
authors.lastname: Li
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Liu
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Liu
mesh_terms: D001547:Benzaldehydes / Q000138:chemical synthesis / Q000737:chemistry; D008239:Lysine / Q000138:chemical synthesis / Q000737:chemistry*; D014452:Ubiquitins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; C402308:NVOC-chloride; D014452:Ubiquitins; D008239:Lysine