[Expression of RhoC and its regulators RhoGDIbeta RhoGDIgamma and their clinical importance in lung squamous cell cancer and adenocarcinoma.].
BACKGROUND
It has been proven that RhoC is known to be overexpressed in tumor. The activities of RhoC are negatively regulated by guanine nucleotide dissociation inhibitor beta,gamma(GDIbeta,gamma), but its role in cancer is quite limited. The purpose of the study is to investigate the expression of RhoC, RhoGDIbeta, RhoGDIgamma and analyze the correlation between RhoC and RhoGDIbeta RhoGDIgamma expression and clinic implication.


METHODS
Western blot and RT-PCR were used to detect RhoC, RhoGDIbeta and RhoGDIgamma protein and mRNA, respectively in 40 lung cancer tissues.


RESULTS
The expression levels of RhoC, RhoGDIbeta and RhoGDIgamma protein and mRNA were more remarkably higher in all tumor samples than those in the normal tissues. The expression levels of RhoC and RhoGDIgamma positively correlated with clinical TNM staging, lymph node metastasis (r =0.659, P <0.05)(r =0.597, P <0.05), and the expression levels of RhoGDIbeta negatively correlate with clinical TNM staging, lymph node metastasis (r =-0.621, P <0.05).


CONCLUSIONS
RhoC, RhoGDIbeta and RhoGDIgamma are correlated with clinical TNM staging and may be correlated with lymph node metastasis of lung cancer.
Liu
Dongming
D
Department of Pathology, College of Basic Medical Sciences, China Medical Uninersity, Shenyang, Liaoning, 110001, China; Affiliated Hospital of Inner Mongolia University For The Nationalities, Tongliao 028000, China.
Xu
Yuexin
Y
Guan
Zhongyan
Z
Duan
Hongmei
H
Wang
Enhua
E
Han
Yuchen
Y