title: Improvement of total lipid and glycerophospholipid recoveries from various food matrices using pressurized liquid extraction.
abstract: The extraction of three major phospholipid (PL) classes contained in soybean, egg yolk, calf brain, and ox liver was investigated by means of two methods. The PL amounts were evaluated. A new method, based on pressurized liquid extraction (PLE), was applied for total lipids (TL), including PL, extraction and compared with a standard liquid extraction method, a modified Folch method. The three PL classes (phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylcholine (PC)) that were recovered in the obtained TL extracts were quantified using HPLC with an evaporative light-scattering detector (ELSD). Using the PLE method, a single extraction allowed a recovery of more than 94% of TL and 96% of each PL class. Two successive extractions could achieve a total recovery of the three studied PL classes. With the modified Folch method, 77-83% of TL, 80-91% of PE, 82-94% of PC, and no more than 78% of PI could be achieved from various food matrices after one extraction. Four successive extractions were necessary to recover the whole TL content and each PL class. Results indicate that PLE is a rapid and efficient lipid extraction system for the broad range of plant and animal tissues.
authors.affiliation: Equipe de Chimie Analytique des Molécules Bioactives, Université de Strasbourg, IPHC-LC4, UMR 7178, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Julie
authors.initials: J
authors.lastname: Le Grandois
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
authors.forename: Minjie
authors.initials: M
authors.lastname: Zhao
authors.forename: Saïd
authors.initials: S
authors.lastname: Ennahar
authors.forename: Françoise
authors.initials: F
authors.lastname: Bindler
mesh_terms: D000818:Animals; D005504:Food Analysis / Q000379:methods*; D020404:Glycerophospholipids / Q000032:analysis / Q000302:isolation & purification*; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D014020:Tissue Extracts / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D020404:Glycerophospholipids; D008055:Lipids; D010936:Plant Extracts; D014020:Tissue Extracts