title: Bioassay-guided isolation of the antidiabetic principle from Sorbus decora (Rosaceae) used traditionally by the Eeyou Istchee Cree First Nations.
abstract: Bioassay-guided fractionation of a crude extract (80% EtOH in H(2)O) of stem bark of Sorbus decora led the isolation of three new pentacycle triterpenes (compounds 1-3). The structures of 1-3 were established on the basis of spectroscopic methods (IR, HREIMS, 1D and 2D NMR) as 23,28-dihydroxyursan-12-ene-3β-caffeate, 23,28-dihydroxylupan-20(29)-ene-3β-caffeate, and 3β,23,28-trihydroxy-12-ursene, respectively. Compound 2 significantly enhanced glucose uptake in C2C12 cells, but compounds 1 and 3 did not. In addition, triterpenoids 4-8, catechin, and epicatechin were also isolated. This is the first comprehensive report of the phytochemical constituents of S. decora since the initial study by Narashmachari and von Rudloff (1962) and includes evaluation of their antidiabetic activity.
authors.affiliation: Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON, K1N6N5, Canada.
authors.forename: José A
authors.initials: JA
authors.lastname: Guerrero-Analco
authors.forename: Louis
authors.initials: L
authors.lastname: Martineau
authors.forename: Ammar
authors.initials: A
authors.lastname: Saleem
authors.forename: Padma
authors.initials: P
authors.lastname: Madiraju
authors.forename: Asim
authors.initials: A
authors.lastname: Muhammad
authors.forename: Tony
authors.initials: T
authors.lastname: Durst
authors.forename: Pierre
authors.initials: P
authors.lastname: Haddad
authors.forename: John Thor
authors.initials: JT
authors.lastname: Arnason
mesh_terms: D000818:Animals; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011792:Quebec; D031994:Sorbus / Q000737:chemistry*; D013237:Stereoisomerism; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C552979:23,28-dihydroxylupan-20(29)-ene-3beta-caffeate; C552978:23,28-dihydroxyursan-12-ene-3beta-caffeate; C552980:3beta,23,28-trihydroxy-12-ursene; D007004:Hypoglycemic Agents; D014315:Triterpenes