title: Expanding role of the jumonji C domain as an RNA hydroxylase.
abstract: JmjC (Jumonji C) domain-containing proteins are known to be an extensive family of Fe(II)/2-oxoglutarate-dependent oxygenases involved in epigenetic regulation of gene expression by catalyzing oxidative demethylation of methylated histones. We report here that a human JmjC protein named Tyw5p (TYW5) unexpectedly acts in the biosynthesis of a hypermodified nucleoside, hydroxywybutosine, in tRNA(Phe) by catalyzing hydroxylation. The finding provides an insight into the expanding role of JmjC protein as an RNA hydroxylase.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Noma
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Ishitani
authors.forename: Megumi
authors.initials: M
authors.lastname: Kato
authors.forename: Asuteka
authors.initials: A
authors.lastname: Nagao
authors.forename: Osamu
authors.initials: O
authors.lastname: Nureki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012360:RNA, Transfer, Phe / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D012360:RNA, Transfer, Phe; C114501:hydroxywybutine; D006147:Guanine; D006899:Mixed Function Oxygenases