title: Detection of circulating tumour cells in peripheral blood of patients with advanced non-metastatic bladder cancer.
abstract: OBJECTIVE
  • To prospectively detect and evaluate the biological significance of circulating tumour cells (CTC) in patients with bladder cancer, especially in those patients with non-metastatic, advanced bladder cancer (NMABC).
  
  
  METHODS
  • Between July 2007 and January 2009, blood samples of 50 consecutive patients with localized bladder cancer and five patients with metastatic disease scheduled for cystectomy were prospectively investigated for CTC. Peripheral blood (7.5 ml) was drawn before cystectomy. • Detection of CTC was performed using the USA Food and Drug Administration-approved CellSearch(TM) system. Data were compared with the clinical and histopathological findings.
  
  
  RESULTS
  • CTC were detected in 15 of 50 patients (30%) with non-metastatic disease and five of five patients with metastatic disease. The overall mean number of CTC was 33.7 (range: 1-372; median: 2). In non-metastatic patients, the mean number of CTC was 3.1 (range: 1-11; median: 1). Except for a univariate association between CTC with vessel infiltration (P= 0.047), all other common clinical and histopathological parameters did not reveal a significant correlation with CTC detection. • A median 1-year follow up was available for 53 patients (96.4%). Ten out of 19 preoperatively CTC-positive patients died as a result of cancer progression. • CTC-positive patients showed significantly worse overall (P = 0.001), progression-free (P < 0.001) and cancer specific survival (P < 0.001) compared to preoperatively CTC-negative patients.
  
  
  CONCLUSIONS
  • This is the largest study demonstrating that detection of CTC in NMABC patients is feasible using the CellSearch(TM) system. Our findings suggest that the presence of CTC may be predictive for early systemic disease.
authors.affiliation: Department of Urology, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany. mrink@uke.uni-hamburg.de
authors.forename: Michael
authors.initials: M
authors.lastname: Rink
authors.forename: Felix K H
authors.initials: FK
authors.lastname: Chun
authors.forename: Sarah
authors.initials: S
authors.lastname: Minner
authors.forename: Martin
authors.initials: M
authors.lastname: Friedrich
authors.forename: Oliver
authors.initials: O
authors.lastname: Mauermann
authors.forename: Hans
authors.initials: H
authors.lastname: Heinzer
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huland
authors.forename: Margit
authors.initials: M
authors.lastname: Fisch
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
authors.forename: Sabine
authors.initials: S
authors.lastname: Riethdorf
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000097:blood / Q000473:pathology*; D055088:Early Detection of Cancer; D004812:Epidemiologic Methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000097:blood / Q000473:pathology*