Cloning and expression analysis of 1 L-myo-inositol-1-phosphate synthase gene from Ricinus communis L.
A novel 1 L-myo-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) gene, designate rcMIPS, was cloned from Ricinus communis. It contained an open reading frame (ORF) of 1669 bp coding for a peptide of 510 amino acids with a molecular mass of 56 kDa. Sequence anaylsis showed high homology compared to other plant MIPS genes, because it contained typical domains owned by MIPS enzymes. The transcript levels of the rcMIPS gene in leaves, stems, and roots were examined after drought stress for 24, 48, and 72 h. The transcript levels in the leaves, stems, and roots increased significantly compared to the control. Results of the enzyme assay showed a significant correlation between the changes of enzyme activity and the transcript levels of the rcMIPS gene in different organs. Decreased relative water contents (RWC) and increased malondialdehyde (MDA) contents in the leaves represented a stress response against drought stress. Our findings suggest that MIPS plays an important role in the defensive mechanisms of R. communis against drought stress.
Wei
Wei
W
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, 610064 Chengdu, PR China.
Dai
Xiao
X
Wang
Yong
Y
Chuan
Yu
Y
Gou
Chun-Bao
CB
Chen
Fang
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D055864:Droughts; D008315:Malondialdehyde / Q000378:metabolism; D008969:Molecular Sequence Data; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics*; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D008315:Malondialdehyde; D007296:Myo-Inositol-1-Phosphate Synthase