Relationship between differential retention of Escherichia coli and Enterococcus faecalis and variations in enzyme activity in the scallop Patinopecten yessoensis.
Uptake of Escherichia coli and Enterococcus faecalis and variations of trypsin amylase activity acid phosphatase and superoxide dismutase in tissue of the scallop Patinopecten yessoensis were detected. The results showed that P. yessoensis accumulated E. faecalis in larger numbers and more rapidly than E. coli, both with the highest concentration in the digestive tract and lowest in hemolymph. Compared to E. coli, all scallops exposed to E. faecalis showed significantly higher trypsin and AMS activity. SOD activity in hemocytes and ACP activity in hemolymph was significantly higher in the treatments with 5 log(10)CFU/ml E. coli than with E. faecalis. But no significant differences in ACP activity of P. yessoensis exposed to a 3 log(10)CFU/ml inoculum of both bacteria were recorded. In conclusion, the mass retention of gut microflora in P. yessoensis is positively correlated with digestive enzymes activity and negatively correlated with ACP activity in the hemocyte.
Li
Bin
B
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Chen
Bijuan
B
Qi
Zhanhui
Z
Jiang
Zengjie
Z
Zhang
Jihong
J
Fang
Jianguang
J
D000818:Animals; D013293:Enterococcus faecalis / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000201:enzymology / Q000382:microbiology; D006434:Hemocytes / Q000201:enzymology / Q000382:microbiology; D049897:Pectinidae / Q000201:enzymology* / Q000382:microbiology*