title: Technical and diagnostic performance of five commercial anti-diphtheria toxoid IgG enzyme-linked immunosorbent assay kits.
abstract: The technical and diagnostic performances of five commercially available enzyme-linked immunosorbent assays for the measurement of anti-diphtheria toxoid IgG antibodies were evaluated. There was good agreement between the relative sensitivities of the five assays, but the relative specificity of one of the assays differed from that of the other four assays. Three of the five assays possessed recoveries of the international reference material NIBSC 00/496 within the range of 90% to 110% at antibody levels >0.1 IU/ml. The data suggest that there are manufacture-dependent differences in relative sensitivity, specificity, and accuracy for the determination of anti-diphtheria toxoid IgG antibodies that could result in different diagnostic interpretations.
authors.affiliation: Binding Site Group Limited, Birmingham, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Faruq
authors.forename: L
authors.initials: L
authors.lastname: Dadson
authors.forename: H
authors.initials: H
authors.lastname: Cox
authors.forename: F
authors.initials: F
authors.lastname: Alcock
authors.forename: A R
authors.initials: AR
authors.lastname: Parker
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000992:Antitoxins / Q000097:blood*; D001431:Bacteriological Techniques / Q000379:methods*; D004165:Diphtheria / Q000175:diagnosis*; D004168:Diphtheria Toxoid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000992:Antitoxins; D004168:Diphtheria Toxoid; D007074:Immunoglobulin G