WT1 immunoprofiling and comparison of malignant Mullerian mixed tumors of the female genital tract.
A malignant Mullerian mixed tumor (MMMT) is a biphasic homologous or heterologous malignancy of the female genital tract. WT1 (Wilms tumor 1) is both a tumor suppressor gene and oncogene overexpressed in the nuclei of some gynecologic carcinomas. Expression in MMMT is incompletely described. Whole sections from 16 MMMTs were stained with WT1 (N terminus) using a standard immunoperoxidase technique. There were 7 heterologous and 9 homologous tumors and 10 were endometrial, 5 were ovarian, and 1 was of peritoneal origin. The tissue and cell staining pattern and score (intensity by amount) were evaluated and correlated with the tumor subtype and anatomic location. Among the 16 tumors, 81.3% showed mostly stromal and cytoplasmic staining and a score of 3 or 6. Staining was positive in 80% of the endometrial and ovarian tumors and the 1 peritoneal tumor and in all heterologous and 66.7% of the homologous tumors. The immunoprofile correlated with tumor subtype but not with anatomic location. Stromal and epithelial staining was more frequent (83.3%) in homologous tumors and differed significantly (P=0.009) from the heterologous types where stromal staining prevailed (85.7%). MMMT is another genital tract malignancy which can over express WT1 and the immunoprofile may assist in tumor subtyping.
Franko
Angela
A
Department of Pathology and Laboratory Medicine Department of Oncology, University of Calgary, Alberta, Canada.
Magliocco
Anthony M
AM
Duan
Quili
Q
Duggan
Máire A
MA
D005260:Female; D005833:Genital Neoplasms, Female / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D018200:Mixed Tumor, Mullerian / Q000378:metabolism* / Q000473:pathology*; D025721:WT1 Proteins / Q000096:biosynthesis*
D025721:WT1 Proteins