[Nucleosome remodeling on the regulatory region of the rat tryptophan dioxygenase (tdo) gene during transcription in vivo].
Two DNase I-hypersensitive regions are identified on the regulatory region of the rat tryptophan dioxygenase (tdo) gene which is expressed tissue-specifically under control ofglucocorticoid hormones. DNase I-hypersensitive regions are identified in position -470 and in the vicinity of the first upstream gene. Micrococcal nuclease digestion pattern of this region shows disturbances in the regular cleavage and appearance of shorter DNA molecules than nucleosomal DNA. However the control experiments demonstrate that the same DNA region could be involved in the regular nucleosome core particles under in vitro reconstitution. Taken together, these data show that the nucleosome array in the regulatory region of the actively transcribed tdo gene in vivo is disturbed.
Chikhirzhina
G I
GI
Nazarova
N Iu
NIu
Chikhirzhina
E V
EV
Romanovskaia
E V
EV
D000818:Animals; D042002:Chromatin Assembly and Disassembly; D003850:Deoxyribonuclease I / Q000737:chemistry; D008099:Liver / Q000201:enzymology; D008297:Male; D009707:Nucleosomes / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014366:Tryptophan Oxygenase / Q000235:genetics*
D009707:Nucleosomes; D014366:Tryptophan Oxygenase; D003850:Deoxyribonuclease I