Lipin proteins form homo- and hetero-oligomers.
Lipin family members (lipin 1, 2 and 3) are bi-functional proteins that dephosphorylate PA (phosphatidic acid) to produce DAG (diacylglycerol) and act in the nucleus to regulate gene expression. Although other components of the triacylglycerol synthesis pathway can form oligomeric complexes, it is unknown whether lipin proteins also exist as oligomers. In the present study, using various approaches, we revealed that lipin 1 formed stable homo-oligomers with itself and hetero-oligomers with lipin 2/3. Both the N- and C-terminal regions of lipin 1 mediate its oligomerization in a head-to-head/tail-to-tail manner. We also show that lipin 1 subcellular localization can be influenced through oligomerization, and the individual lipin 1 monomers in the oligomer function independently in catalysing dephosphorylation of PA. The present study provides evidence that lipin proteins function as oligomeric complexes and that the three mammalian lipin isoforms can form combinatorial units.
Liu
Guang-Hui
GH
Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. gliu@salk.edu
Qu
Jing
J
Carmack
Anne E
AE
Kim
Hyun Bae
HB
Chen
Chang
C
Ren
Hongmei
H
Morris
Andrew J
AJ
Finck
Brian N
BN
Harris
Thurl E
TE
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008856:Microscopy, Fluorescence; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010711:Phosphatidate Phosphatase; D010712:Phosphatidic Acids / Q000378:metabolism; D010766:Phosphorylation; D055503:Protein Multimerization; D021381:Protein Transport; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D018411:Spodoptera; D014162:Transfection
C502575:LPIN2 protein, human; D009687:Nuclear Proteins; D010712:Phosphatidic Acids; D011994:Recombinant Proteins; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase