[Effect of photodynamic therapy on the proliferation and cell distribution of hypertrophic scar fibroblast].
OBJECTIVE
To investigate the effect of HMME-PDT on the proliferation and cell distribution of hypertrophic scar fibroblast (HSF).


METHODS
HSF were cultured and only 4-6th passages were used in this study. Argyrophilic protein in nucleolar organizer regions(AgNORs) were calculated by I. S% after argyrophilic staining. Flow cytometry was applied to analyze the cell cycle and proliferation index (PI).


RESULTS
1) I. S% of HSF after HMME-PDT was reduced markedly. 2) HMME-PDT inhibited HSF entering S stage from G, stage, cell percentage in S stage was decreased to (11.2 +/- 2.3)%. 3) PI in HMME-PDT group was less than that in control group [(35.0 +/- 3.4)% vs (27.2 +/- 3.1)%, P < 0.05].


CONCLUSIONS
HMME-PDT can inhibit proliferation of HSF, and chang cell distribution.
Cai
Hong
H
Department of Physical Therapy, the 316th Chinese Hospital of PLA, Beijing 100093, China.
Gu
Ying
Y
Sun
Qiang
Q
Zeng
Jing
J
Li
Shi-Long
SL
D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000188:drug therapy / Q000473:pathology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D010778:Photochemotherapy