Factors affecting mark quality of alizarin complexone-stained otoliths in juvenile black bream Acanthopagrus butcheri and a prescription for dosage.
The relationships between otolith score, a function of increasing stain visibility, and the ratio of alizarin complexone (mg) to fish biomass (g) for 0.1, 0.6 and 7 g black bream Acanthopagrus butcheri show that acceptable marks are produced in all size classes using a stain-to-biomass ratio of 3.3 mg g(-1) following an immersion period of 24 h.
Partridge
G J
GJ
Aquaculture Development Unit, WA Maritime Training Centre, 1 Fleet Street, Fremantle, Western Australia 6160, Australia. gavin.partridge@challengertafe.wa.edu.au
Jenkins
G I
GI
Doupé
R G
RG
De Lestang
S
S
Ginbey
B M
BM
French
D
D
D000704:Analysis of Variance; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D005398:Fisheries / Q000379:methods*; D010037:Otolithic Membrane / Q000737:chemistry*; D010473:Perciformes / Q000502:physiology*; D011156:Population Density; D013194:Staining and Labeling / Q000379:methods*
D000880:Anthraquinones; C070804:alizarin complexone