title: Inhibition of TNF-α-mediated endothelial cell-monocyte cell adhesion and adhesion molecules expression by the resveratrol derivative, trans-3,5,4'-trimethoxystilbene.
abstract: Resveratrol (RSV) has been shown to have anti-inflammatory activity and to have a protective role against atherosclerosis. Here it is shown, for the first time, that its derivative trans-3,5,4'-trimethoxystilbene (TMS) may be a more potent anti-inflammatory agent than resveratrol. A comparative analysis of the inhibitory activities of related stilbenes, resveratrol, TMS and polydatin (PD), on monocyte adhesion to TNF-α-activated endothelial cells showed TMS to be the most effective, with PD being the least effective. RSV and its analogues inhibited, albeit differentially, the protein and mRNA expression levels of inducible cell adhesion molecules, ICAM-1 and VCAM-1, in cultured endothelial cells. The mechanism of the inhibitory effects of these stilbenes on endothelial cell-monocyte cell adhesion can be attributed mainly to inhibition of NF-κB pathway activation. The results demonstrate that all three investigated stilbene compounds, especially TMS, exhibit a potent inhibitory effect on inflammation-induced cell-cell adhesion, expression of adhesion molecules and activation of the NF-κB pathway.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China.
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Deng
authors.forename: Deepa
authors.initials: D
authors.lastname: Alex
authors.forename: He-Qing
authors.initials: HQ
authors.lastname: Huang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Yu
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
authors.forename: George P H
authors.initials: GP
authors.lastname: Leung
authors.forename: Simon Ming-Yuen
authors.initials: SM
authors.lastname: Lee
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D015394:Molecular Structure; D009000:Monocytes / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: C497286:3,4',5-trimethoxystilbene; D005960:Glucosides; D016328:NF-kappa B; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D000077185:Resveratrol; C058229:polydatin