title: Tissue-response to calcium-bonded titanium surface.
abstract: Our previous study demonstrated that calcium-bonded titanium surface (Ca-Ti) can be obtained by hydrothermal reaction between titanium (Ti) and CaCl(2) and that bone-apatite like formation was observed after immersion in simulated body fluid. The purpose of the study was to determine the in vivo response to Ca-Ti surface using a rodent tibia model. Cylinders of commercially pure Ti were divided into three groups: (1) untreated group; (2) NaOH+hTi group: soaked in 5 mol/L NaOH solution at 60 degrees C then heated at 400 degrees C for 1 h; and (3) Ca-Ti group: hydrothermally treated in the presence of 10 mmol/L CaCl(2) at 200 degrees C for 24 h. The cylinders implanted in surgically created defects in tibias of 8-week old male Wistar rats were retrieved after 1, 2, and 4 weeks. Histomorphometric evaluations were made on stained decalcified thin sections. Results showed that at 1, 2, and 4 week after implantation, respectively, bone contact was 55.2 +/- 16.4%, 88.1 +/- 9.9%, and 96.1 +/- 4.8% for Ca-Ti implants, 5.7 +/- 5.3%, 19.9 +/- 1.2%, 57.4 +/- 4.8% for untreated; and 27.2 +/- 0.7%, 70.9 +/- 7.7%, and 96.0 +/- 5.1% for NaOH+hTi implants. These results suggest that hydrothermal treatment with CaCl(2) provides a bioactive Ca-Ti bonded surface that allows bone formation greater than that obtained with NaOH+heat treated Ti surfaces.
authors.affiliation: Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. zhanglei@dent.kyushu-u.ac.jp
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ayukawa
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: Legeros
authors.forename: Shigeki
authors.initials: S
authors.lastname: Matsuya
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koyano
authors.forename: Kunio
authors.initials: K
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002118:Calcium / Q000494:pharmacology*; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
chemical_list: D014025:Titanium; D002118:Calcium