In vitro expansion of autonomously contracting, cardiomyogenic structures from rainbow trout Oncorhynchus mykiss.
In primary cell preparations from larvae of rainbow trout Oncorhynchus mykiss, the formation of autonomously contracting cell aggregates was observed after 7 days. These contracting elements could be propagated and some aggregates were maintained over a period of 35 days. Electron microscopical and immunocytochemical examination revealed the presence of cardiomyocytes.
Grunow
B
B
Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Paul Ehrlich Str. 1-3, 23562, Lübeck, Germany. bianka.grunow@emb.fraunhofer.de
Ciba
P
P
Rakers
S
S
Klinger
M
M
Anders
E
E
Kruse
C
C
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D046529:Microscopy, Electron, Transmission; D009119:Muscle Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology