Effect of 8-O-acetyl shanzhiside methylester increases angiogenesis and improves functional recovery after stroke.
We investigated whether 8-O-acetyl shanzhiside methylester (ND01) regulates angiogenesis and thereby improves functional outcome after stroke. Adult male rats were subjected to 1 hr of middle cerebral artery occlusion (MCAO) and reperfusion, and treated with or without different doses (5 and 10 mg/kg) of ND01, starting 24 hr after ischaemia and reperfusion (I/R) and by intravenous injection daily for 14 days. Neurological functional tests were performed and cerebral Evans blue extravasation was measured. Angiogenesis and angiogenic factor expression were measured by immunohistochemistry and Western blot, respectively. The results indicated that ND01 significantly promoted angiogenesis in the ischaemic brain and improved functional outcome after stroke. ND01 also significantly increased vascularization compared with vehicle treatment. ND01 increased the expression of VEGF, Ang1, phosphorylation of Tie2 and Akt VEGF. The Ang1/Tie2 axis and Akt pathways appear to mediate ND01-induced angiogenesis.
Jiang
Wang-Lin
WL
College of Pharmacy and Institute of Material Medica, Binzhou Medical University, Yantai, China.
Zhang
Shu-Ping
SP
Zhu
Hai-Bo
HB
Hou
Jian
J
D043925:Angiogenesis Inducing Agents / Q000627:therapeutic use*; D042501:Angiogenic Proteins / Q000378:metabolism; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism; D005960:Glucosides / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D011714:Pyrans / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000188:drug therapy* / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D042501:Angiogenic Proteins; D005960:Glucosides; D011714:Pyrans; C099473:8-O-acetyl shanzhiside methyl ester