[The expression of ABCG4, V-ATPase and clinic significance of their correlation with NSCLC.].
BACKGROUND
It has been proven that the multiple drug resistance is main reason for failure of chemotherapy in lung cancers and ABC transporter play a main role for chemoresistance in mediating drug efflux. So searching for new drug resistant protein of the ABC family and elucidating its resistant mechanism is very important. ABCG4 is one of ABC family and is expected to be candidate drug resistant protein; and the drug resistance probably correlated with pH value around cancer cell, while, V-ATPase play key role in modulating the pH. So our aim is to investigate the expressions of ABCG4, V-ATPase proteins in non small cell lung cancer (NSCLC), and analyze relationship of ABCG4, V-ATPase protein expressional rate in these cancers with the cancers' pathological grade and TNM stages.


METHODS
To detect the expression rates of ABCG4, V-ATPase protein in NSCLC with immunohistochemical method and immuno- fluorescent method, and to observe the location, the collocation of the proteins under light microscope and confocal laser scanning microscope; the differences of the protein expression and their correlations were analyzed by statistics.


RESULTS
ABCG4 protein was high expressed in squamous cell lung cancer, lung adenocarcinoma respectively, and between the two kinds of the cancers there was a significant difference (P =0.001) for their comparison; there were significant differences between pathological grade II and II-III of squamous cell lung cancer, between different differentiated lung adenocarcinoma. V-ATPase protein were also high expressed in these two kinds of cancers, and there was significant difference for their comparison; there were significant differences between pathological grade II and II-III of squamous cell lung cancer, between different differentiated lung adenocarcinoma; there were no significant differences among the squamous cell lung cancer and lung adenocarcinoma for TNM stages respectively. The P values of correlationship test of positive intensity between ABCG4 and V-ATPase expression in the total squamous cell lung cancer and lung adenocarcinoma, in the pathological grade II and II-III of the squamous cell lung cancer, in the moderately differentiated lung adenocarcinoma were all 0.000, while the correlation coefficients were 0.771, 0.765, 0.714, 0.777, 0.865 respectively; however, the P values of correlationship test of between ABCG4 and V-ATPase expression in the low differentiated lung adenocarcinoma was 0.048, and the correlation coefficient was 0.35. The ABCG4 and V-ATPase positive expression was mainly located in the plasma membrane and cytoplasm, and there was collocation among the ABCG4 and V-ATPase proteins.


CONCLUSIONS
The ABCG4 and V-ATPase proteins are highly expressed in the NSCLC, and the expression rates of ABCG4, V-ATPase proteins are correlated with the cancers' pathological grades and TNM stage; and there are all having correlationship between ABCG4 and V-ATPase proteins in the squamous cell lung cancer, in the lung adenocarcinoma, in all their pathological grades.
Zhang
Zhipei
Z
Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Li
Xiaofei
X
Wang
Xiaoping
X
Zhou
Yong'an
Y
Xu
Hong
H
Wang
Jian
J
Huang
Lijun
L
Tian
Yutu
Y
Cheng
Qingshu
Q