[Effect of von Willebrand factor on the biological characteristics of colorectal cancer cells].
OBJECTIVE
To study effect of von Willebrand factor (vWF) on the proliferation, adhesion and migration of human colorectal cancer cells.


METHODS
Human colorectal cancer cell line SW480 was cultured in vitro, and the expression of vWF in SW480 cells was detected by immunocytochemistry. SW480 cells were treated with vWF antibody (vWFAb), and the morphological change was examined by inverted microscope. Cell proliferation and ability to adhere extracellular matrix IIII( collagen were detected with MTT. Migration ability of SW480 cells was assayed by Transwell.


RESULTS
The human colorectal cancer cell line SW480 expressed vWF which was mainly in nucleus and slightly in cytoplasm. vWFAb significantly inhibited the proliferation ability of SW480 cells in dose- and time-dependent manner (P<0.05). After vWFAb treatment, SW480 cells adhesion decreased significantly (P<0.05), and transmembrane migration of cells significantly decreased (54.60+/-11.01 vs 97.27+/-10.01, P<0.01).


CONCLUSIONS
Human colorectal cancer cells can express vWF. vWF in human colorectal cancer cells plays an important role in promoting proliferation, adhesion, and migration.
Liu
Gang
G
Department of General Surgery, The Second Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China. liugang20081112@163.com
Ren
Yuan-man
YM
D017209:Apoptosis; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009362:Neoplasm Metastasis; D014841:von Willebrand Factor / Q000378:metabolism*
D014841:von Willebrand Factor