title: Electrospun poly(ɛ-caprolactone) scaffold for suture-free solder-mediated laser-assisted vessel repair.
abstract: OBJECTIVE
  The addition of poly(lactic-co-glycolic) acid (PLGA) scaffolds to liquid solder-mediated laser-assisted vascular repair (sLAVR) has been shown to increase soldering strength significantly. Unfortunately, the fast degradation of PLGA is associated with adverse effects such as acidity of the degradation products. This study investigated the possibility of using electrospun poly(ɛ-caprolactone) (PCL) as reinforcement material in scaffold and solder-mediated LAVR (ssLAVR).
  
  
  METHODS
  In vitro sLAVR of 10-mm arteriotomies (n = 62) was performed on 0.3- to 0.6-cm diameter porcine carotid arteries with a 670-nm diode laser. The solder contained 50% bovine serum albumin (BSA) and 0.1-0.7% methylene blue (MB) as a chromophore. The soldering strength was studied as a function of PCL-scaffold thickness, scaffold-fiber diameter, MB concentration, number of laser passes, and different sLAVR techniques. Leaking-point pressures (LPPs) were measured with a fluid-infusion technique.
  
  
  RESULTS
  The highest mean ± SD LPP (749 ± 171 mm Hg) was produced by the ssLAVR modality that included the sheathing of the arteriotomy with 30 μL solder containing 50% BSA and 0.5% MB, followed by application of the PCL scaffold (mean ± SD thickness of 187 ± 9 μm and 14-μm fiber diameter) and irradiation with two consecutive continuous-wave laser passes.
  
  
  CONCLUSIONS
  The study demonstrated the potential applicability of an electrospun PCL scaffold as reinforcement material in ssLAVR. Soldering strength was dependent on the scaffold physical properties, chromophore concentration, the number of laser passes, and the ssLAVR technique.
authors.affiliation: Department of Surgery, Academic Medical Center, Amsterdam, the Netherlands.
authors.forename: Dara R
authors.initials: DR
authors.lastname: Pabittei
authors.forename: Michal
authors.initials: M
authors.lastname: Heger
authors.forename: Ron
authors.initials: R
authors.lastname: Balm
authors.forename: Han E H
authors.initials: HE
authors.lastname: Meijer
authors.forename: Bas
authors.initials: B
authors.lastname: de Mol
authors.forename: Johan F
authors.initials: JF
authors.lastname: Beek
mesh_terms: D000818:Animals; D002208:Caproates; D002339:Carotid Arteries / Q000601:surgery; D066298:In Vitro Techniques; D019344:Lactic Acid; D007783:Lactones; D007834:Lasers; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D054457:Tissue Scaffolds; D014656:Vascular Surgical Procedures / Q000379:methods*
chemical_list: D002208:Caproates; D007783:Lactones; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C121056:caprolactone