title: Molecular and biochemical mining of heat-shock and 14-3-3 proteins in drug-induced protoscolices of Echinococcus granulosus and the detection of a candidate gene for anthelmintic resistance.
abstract: Cystic echinococcosis (CE) caused by the larval stage of Echinococcus granulosus is a disease that affects both humans and animals. In humans the disease is treated by surgery with a supplementary option of chemotherapy with a benzimidazole compound. During the present study heat-shock protein 60 (HSP 60) was identified as one of the most frequently expressed biomolecules by E. granulosus after albendazole treatment. Data were correlated with 14-3-3 protein signature, and overexpression of this molecule after albendazole induction was an indicator of cell survival and signal transduction during in vitro maintenance of E. granulosus for up to 72 h. This observation was further correlated with a uniform expression pattern of a housekeeping gene (actin II). Out of three β-tubulin gene isoforms of E. granulosus, β-tubulin gene isoform 2 showed a conserved point mutation indicative of benzimidazole resistance.
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: D
authors.initials: D
authors.lastname: Pan
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Bera
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: S
authors.initials: S
authors.lastname: Bandyopadhyay
authors.affiliation: NRC on Yak, Arunachal Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Bandyopadhyay
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: S
authors.initials: S
authors.lastname: De
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: T
authors.initials: T
authors.lastname: Rana
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Das
authors.affiliation: Indian Veterinary Research Institute, FMD Research Laboratory, Bangalore Campus, Hebbal, Bangalore 560 024, India.
authors.forename: V V
authors.initials: VV
authors.lastname: Suryanaryana
authors.affiliation: Department of Chest Medicine, RG Kar Medical College and Hospital, Kolkata, West Bengal, India.
authors.forename: J
authors.initials: J
authors.lastname: Deb
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata 700 037, India.
authors.forename: D
authors.initials: D
authors.lastname: Bhattacharya
mesh_terms: D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics*; D004351:Drug Resistance; D048209:Echinococcus granulosus / Q000187:drug effects* / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics; D017354:Point Mutation; D014404:Tubulin / Q000235:genetics
chemical_list: D048948:14-3-3 Proteins; D000871:Anthelmintics; D018834:Chaperonin 60; D050505:Mutant Proteins; D014404:Tubulin