Structural evidence of glycoprotein assembly in cellular membrane compartments prior to Alphavirus budding.
Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production.
Soonsawad
Pan
P
Department of Molecular and Cellular Biology, University of California, Davis, CA 95616-8536, USA.
Xing
Li
L
Milla
Emerson
E
Espinoza
Juan M
JM
Kawano
Masaaki
M
Marko
Michael
M
Hsieh
Chyongere
C
Furukawa
Hiromitsu
H
Kawasaki
Masahiro
M
Weerachatyanukul
Wattana
W
Srivastava
Ranjana
R
Barnett
Susan W
SW
Srivastava
Indresh K
IK
Cheng
R Holland
RH
D000524:Alphavirus / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology*; D006224:Cricetinae; D055032:Electron Microscope Tomography; D006023:Glycoproteins / Q000378:metabolism*; D014617:Vacuoles; D014764:Viral Proteins / Q000378:metabolism; D019065:Virus Assembly; D057074:Virus Release
D006023:Glycoproteins; D014764:Viral Proteins