Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers.
A molecular approach, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.
Vergara-Chen
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C
Departamento de Ecología e Hidrología, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain. carlos.vergara@alu.um.es
González-Wangüemert
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M
Bermingham
E
E
D'Croz
L
L
D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D005398:Fisheries / Q000379:methods*; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010473:Perciformes / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D045303:Cytochromes b