title: Production of monoclonal antibodies to P-glycoprotein: its application in detection of soluble and surface P-glycoprotein of leukemia patients.
abstract: Multidrug resistance (MDR) in leukemia is commonly associated with the expression of a transmembrane protein, P-glycoprotein (P-gp). In this study, two monoclonal antibodies (mAbs) specific for the extracellular domain of P-gp were generated. By employing the generated mAbs, a two-color lysed whole blood flow cytometric method for surface P-gp and an efficient sandwich ELISA for soluble P-gp determinations were established. By using the established methods, surface and soluble P-gp were detected in several leukemia patients. The presence of soluble P-gp could be used to identify the P-gp surface expression patients. Detection of soluble P-gp reported provides a new basis that may lead to a better understanding of the MDR mechanism in leukemia.
authors.affiliation: Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Sawitree
authors.initials: S
authors.lastname: Chiampanichayakul
authors.forename: Songyot
authors.initials: S
authors.lastname: Anuchapreeda
authors.forename: Nuttapol
authors.initials: N
authors.lastname: Chruewkamlow
authors.forename: Kodchakorn
authors.initials: K
authors.lastname: Mahasongkram
authors.forename: Pattra
authors.initials: P
authors.lastname: Thanaratanakorn
authors.forename: Watchara
authors.initials: W
authors.lastname: Kasinrerk
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001798:Blood Proteins / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis; D008722:Methods
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D008562:Membrane Glycoproteins