title: Keto-carotenoids are the major metabolites of dietary lutein and fucoxanthin in mouse tissues.
abstract: Fucoxanthin, a xanthophyll present in brown algae consumed in Eastern Asia, can suppress carcinogenesis and obesity in rodents. We investigated the metabolism, tissue distribution, and depletion of fucoxanthin in ICR mice by comparison with those of lutein. The experiments comprised 14-d dietary supplementation with lutein esters or fucoxanthin, followed by 41- or 28-d, respectively, depletion periods with carotenoid-free diets. After lutein ester supplementation, 3'-hydroxy-ε,ε-caroten-3-one and lutein were the predominant carotenoids in plasma and tissues, accompanied by ε,ε-carotene-3,3'-dione. The presence of these keto-carotenoids in mouse tissues is reported here for the first time, to our knowledge. Lutein and its metabolites accumulated most in the liver (7.51 μmol/kg), followed by plasma (2.11 μmol/L), adipose tissues (1.01-1.44 μmol/kg), and kidney (0.87 μmol/kg). The half-life of the depletion (t(1/2)) of lutein metabolites varied as follows: plasma (1.16 d) < liver (2.63 d) < kidney (4.44 d) < < < adipose tissues (>41 d). Fucoxanthinol and amarouciaxanthin A were the main metabolites in mice fed fucoxanthin and partitioned more into adipose tissues (3.13-3.64 μmol/kg) than into plasma, liver, and kidney (1.29-1.80 μmol/kg). Fucoxanthin metabolites had shorter t(1/2) in plasma, liver, and kidneys (0.92-1.23 d) compared with those of adipose tissues (2.76-4.81 d). The tissue distribution of lutein and fucoxanthin metabolites was not associated with their lipophilicity, but depletion seemed to be slower for more lipophilic compounds. We concluded that mice actively convert lutein and fucoxanthin to keto-carotenoids by oxidizing the secondary hydroxyl groups and accumulate them in tissues.
authors.affiliation: National Food Research Institute, NARO, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Lina
authors.initials: L
authors.lastname: Yonekura
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Terasaki
authors.forename: Akihiko
authors.initials: A
authors.lastname: Nagao
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002338:Carotenoids / Q000032:analysis* / Q000097:blood; D019587:Dietary Supplements; D004952:Esters / Q000008:administration & dosage; D006207:Half-Life; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D014975:Lutein / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D024341:Xanthophylls / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: C552883:3'-hydroxy-epsilon,epsilon-caroten-3-one; D004952:Esters; D024341:Xanthophylls; C025164:fucoxanthin; D002338:Carotenoids; D014975:Lutein