Quantitative determination of sparfloxacin in rat plasma by liquid chromatography/tandem mass spectrometry.
Sparfloxacin, a fluoroquinolone antibiotic, is used for the treatment of bacterial infection. A quantification method using mass spectrometry was developed for the determination of sparfloxacin in rat plasma. After simple protein precipitation with acetonitrile, the analytes were chromatographed on a reversed-phase C₁₈ column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma sparfloxacin concentrations after a single oral administration of sparfloxacin in rats.
Noh
Keumhan
K
College of Pharmacy, Catholic University of Daegu, Kyoungbuk 712-702, South Korea.
Kwon
Kwang-Il
KI
Jeong
Tae Cheon
TC
Kang
Wonku
W
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; C061363:sparfloxacin