Interaction of PqqE and PqqD in the pyrroloquinoline quinone (PQQ) biosynthetic pathway links PqqD to the radical SAM superfamily.
pqqD is one of six genes required for PQQ production in Klebsiella pneumoniae. Herein, we demonstrate that PqqD interacts specifically with the radical SAM enzyme PqqE, causing a perturbation in the electronic environment around the [4Fe-4S](+) clusters. This interaction redirects the role for PqqD in PQQ biosynthesis.
Wecksler
Stephen R
SR
Department of Chemistry, Department of Molecular and Cell Biology, and the California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.
Stoll
Stefan
S
Iavarone
Anthony T
AT
Imsand
Erin M
EM
Tran
Ha
H
Britt
R David
RD
Klinman
Judith P
JP
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology; D045542:PQQ Cofactor / Q000096:biosynthesis* / Q000737:chemistry; D012436:S-Adenosylmethionine / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D005609:Free Radicals; D045542:PQQ Cofactor; D012436:S-Adenosylmethionine