Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.).
Starch paste viscosity plays an important role in estimating the cooking, eating, and processing quality of rice. The inheritance of starch paste viscosity in glutinous rice remains undefined. In the present study, 118 glutinous rice accessions were collected, and the genotypes of 17 starch synthesis-related genes (SSRG) were analyzed by using 43 gene-specific molecular markers. Association analysis indicated that 10 of 17 SSRGs were involved in controlling the rapid visco analyzer (RVA) profile parameters. Among these, the PUL gene was identified to play an important role in control of peak viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), peak time (PeT), and paste temperature (PaT) in glutinous rice. Other SSRGs involved only a few RVA profile parameters. Furthermore, interactions between SSRGs were found being responsible for PeT, PaT, and BDV. Some of the RVA parameters, including PKV, HPV, CPV, CSV, and PaT, were mainly governed by single SSRG, whereas other parameters, such as BDV, SBV, and PeT, were controlled by a few SSRGs, functioning cooperatively. Further, three near-isogenic lines (NIL) of a japonica glutinous cv. Suyunuo as genetic background, with PUL, SSIII-1, and SSIII-2 alleles replaced with those of indica cv. Guichao 2, were employed to verify the genetic effects of the various genes, and the results were consistent with those obtained from the association analysis. These findings indicated that starch paste viscosity in glutinous rice had a complex genetic system, and the PUL gene played an important role in determining the RVA profile parameters in glutinous rice. These results provide important information for potentially improving the quality of glutinous rice.
Yan
Chang-Jie
CJ
Jiangsu Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics, Ministry of Education of China, Agricultural College of Yangzhou University, 12 East Wenhui Road, Jiangsu, 225009, People's Republic of China.
Tian
Zhi-Xi
ZX
Fang
Yu-Wei
YW
Yang
Ya-Chun
YC
Li
Jian
J
Zeng
Sheng-Yuan
SY
Gu
Shi-Liang
SL
Xu
Chen-Wu
CW
Tang
Shu-Zhu
SZ
Gu
Ming-Hong
MH
D017343:Genes, Plant / Q000235:genetics*; D056726:Genetic Association Studies; D007178:Inbreeding; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D011157:Population Dynamics; D015203:Reproducibility of Results; D013213:Starch / Q000096:biosynthesis / Q000737:chemistry*; D014783:Viscosity
D013213:Starch