Excitatory glycine responses of CNS myelin mediated by NR1/NR3 "NMDA" receptor subunits.
NMDA receptors are typically excited by a combination of glutamate and glycine. Here we describe excitatory responses in CNS myelin that are gated by a glycine agonist alone and mediated by NR1/NR3 "NMDA" receptor subunits. Response properties include activation by d-serine, inhibition by the glycine-site antagonist CNQX, and insensitivity to the glutamate-site antagonist d-APV. d-Serine responses were abrogated in NR3A-deficient mice. Our results suggest the presence of functional NR1/NR3 receptors in CNS myelin.
Piña-Crespo
Juan C
JC
Molecular Neurobiology Laboratory, Salk Institute, and Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, La Jolla, California 92037, USA.
Talantova
Maria
M
Micu
Ileana
I
States
Bradley
B
Chen
H-S Vincent
HS
Tu
Shichun
S
Nakanishi
Nobuki
N
Tong
Gary
G
Zhang
Dongxian
D
Heinemann
Stephen F
SF
Zamponi
Gerald W
GW
Stys
Peter K
PK
Lipton
Stuart A
SA
D000818:Animals; D002460:Cell Line; D002490:Central Nervous System / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D005998:Glycine / Q000502:physiology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000502:physiology*; D021122:Protein Subunits / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000819:agonists / Q000494:pharmacology
C409466:NR1 NMDA receptor; C464437:NR3A NMDA receptor; D021122:Protein Subunits; D016194:Receptors, N-Methyl-D-Aspartate; D011994:Recombinant Proteins; D005998:Glycine