An updated and simplified method for bile duct cannulation of rats.
The single bile duct cannulated rat model was used for short-term intestinal absorption studies. Rats were randomly assigned to three groups: (A) bile duct cannulated, (B) control laparotomy and (C) no treatment. The body weight and health of the rats were monitored before and until day 5 after surgery, while bile flow was measured in group A on day 2. On the fifth day, tail vein blood was harvested, and alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin were quantified. Analysis of variance showed no significant difference between the groups for these parameters. This study demonstrated the suitability of a surgical rat model feasible for evaluation of the impact of bile in pharmacokinetic studies.
Tønsberg
H
H
Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
Holm
R
R
Bjerregaard
T G
TG
Boll
J B
JB
Jacobsen
J
J
Müllertz
A
A
D000818:Animals; D001652:Bile Ducts / Q000601:surgery*; D002404:Catheterization / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D007408:Intestinal Absorption / Q000502:physiology; D007756:Laboratory Animal Science / Q000379:methods*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley