title: A new amido phosphonate derivative of carboxymethylcellulose with an osteogenic activity and which is capable of interacting with any Ti surface.
abstract: A new phosphonate derivative of carboxymethylcellulose (CMC) was recently synthesized (CMCAPh). The phosphonate polysaccharide was obtained by using a carbodiimide-like activating agent for carboxylic groups and 2-aminoethyl-phosphonic acid to create an amide bond between the amine of the phosphonate agent and the carboxylic acids of CMC. The polymer was characterized by (31)P NMR, FT-IR, and potentiometric titration. CMCAPh showed different properties from CMC and its amidated derivative polymer CMCA. The behavior in solution of CMCAPh polymer towards normal human osteoblasts (NHOst) was studied in vitro, monitoring the cell proliferation, cell differentiation, and osteogenic activity and was then compared with the amidic derivative of carboxymethylcellulose (CMCA). Furthermore, CMCAPh was used to coat titania disks with the aim of increasing the osteogenic activity of implant surfaces. The polymer film on the titania surface was characterized by AFM and TOF-SIMS analysis. An ATR FT-IR study was carried out to evaluate the polymer bonding mode onto the titanium surface. Osteoblast morphology was evaluated by SEM. Adhesion analysis of NHOst demonstrated a better adhesion on the titanium surface coated with CMCAPh than on the bare titanium surface.
authors.affiliation: Department of Pure and Applied Medicinal Chemistry and C.R.I.S.M.A., University of Siena, Via A. Moro 2, Siena 53100, Italy. barbucci@unisi.it
authors.forename: Rolando
authors.initials: R
authors.lastname: Barbucci
authors.forename: Enrico
authors.initials: E
authors.lastname: Arturoni
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Panariello
authors.forename: Clara
authors.initials: C
authors.lastname: Di Canio
authors.forename: Stefania
authors.initials: S
authors.lastname: Lamponi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D063065:Organophosphonates / Q000737:chemistry / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects; D013696:Temperature; D014025:Titanium / Q000494:pharmacology*; D014783:Viscosity / Q000187:drug effects
chemical_list: D063065:Organophosphonates; D012996:Solutions; D014025:Titanium; D000469:Alkaline Phosphatase; D002266:Carboxymethylcellulose Sodium