In vitro effect of sodium fluoride on antioxidative enzymes and apoptosis during murine odontogenesis.
Excessive fluoride ingestion has been identified as a risk factor for fluorosis and oxidative stress. The oxidative stress results from the loss of equilibrium between oxidative and antioxidative mechanisms that can produce kinase activation, mitochondrial disturbance and DNA fragmentation, resulting in apoptosis. Actually many people are exposed to no-adverted fluoride consumption in acute or chronic way. The aim of this study was to determine the effect of sodium fluoride on first molar germ in relation to its effect on antioxidative enzymes immunoexpression and apoptosis. Thirty first molar germs from 1-day-old Balb/c mice were cultured for 24 h with sodium fluoride (0 mM, 1 mM and 5 mM). Immunoexpression determination of CuZnSod, MnSod, catalase, Bax, Bid, caspase 8, caspase 9, caspase 3 and TUNEL assay were performed. Cellular disorganization in ameloblast and odontoblast-papilla zones was observed. CuZnSod and MnSod immunoexpression decrease in experimental groups. Caspase 8, caspase 3, Bax, Bid increase expression and more TUNEL positive cells in both experimental groups than control, suggest that apoptosis induced by fluoride is related to oxidative stress due to reduction of the enzymatic antioxidant.
Jacinto-Alemán
L F
LF
Laboratorio de Inmunología, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico. inmuno1@live.com.mx
Hernández-Guerrero
J C
JC
Trejo-Solís
C
C
Jiménez-Farfán
M D
MD
Fernández-Presas
A M
AM
D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051023:BH3 Interacting Domain Death Agonist Protein / Q000096:biosynthesis; D002327:Cariostatic Agents / Q000633:toxicity*; D020169:Caspases / Q000096:biosynthesis; D002374:Catalase / Q000096:biosynthesis; D020287:In Situ Nick-End Labeling; D051379:Mice; D008807:Mice, Inbred BALB C; D009805:Odontogenesis / Q000187:drug effects*; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014083:Tooth Germ / Q000187:drug effects* / Q000201:enzymology; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
D051023:BH3 Interacting Domain Death Agonist Protein; C494815:Bid protein, mouse; D002327:Cariostatic Agents; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D012969:Sodium Fluoride; D002374:Catalase; D013482:Superoxide Dismutase; D020169:Caspases