[The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer.].
BACKGROUND
It has been confirmed that nm23-H1 gene is one of the tumor metastasis suppressor genes. Up to now, the exact mechanism of nm23-H1 gebe is uncertain. The aim of this study the mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer.


METHODS
The wild and mutant type of pEGFP-nm23-H1 plasmids [WT (wild type), H118F, S120G, P96S, S44A] were transfected into the L9981 lung cancer cell lines through liposome method, and the complex of KSR and nm23-H1 was detected through co-immunoprecipitation and Western blot assay.


RESULTS
The human KSR could be detected in the nm23-H1 immunoprecipitations in all the trasfected L9981 lung cancer cell lines. But no significant difference of KSR expression was found in the wild and mutant nm23-H1 trasfected cell lines (F =0.190, P =0.938).


CONCLUSIONS
There was a close interaction between nm23-H1 and KSR, which was independent of the nm23-H1 mutation. Nm23-H1 involving in the Ras signaling of lung cancer may be through the KSR gene.
Yang
Xueqin
X
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Yao
Yibing
Y
Zhu
Wen
W
Ma
Li
L
Zhu
Daxing
D
Xiao
Wen
W
Li
Changlin
C
Ma
Jiabao
J
Zhang
Zhixuan
Z
Gao
Liwei
L
Wang
Yanping
Y
Zhou
Qinghua
Q