Folic acid-targeted mesoporous silica nanoparticles for two-photon fluorescence.
New nanotools for the imaging of cancer cells have been synthesized. Two-photon dye-doped 3-aminopropyltriethoxysilane-grafted mesoporous silica nanoparticles (MSN) have been grafted with folic acid (FA) functionalized PEG groups. Amine-PEG groups were first reacted with an activated ester derivative of FA. A mixture of FA- and hydroxyl-PEG has then been reacted with the amino groups at the surface of the particles. Cell culture experiments performed with MCF7 and HeLa cancer cells demonstrated that these functionalized MSN showed a low cytotoxicity even after a 24 hours incubation time at high concentrations. These modified MSN are promising for applications in the field of two-photon imaging and their potentiality for photodynamic therapy is currently being investigated.
Lebret
Valérie
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Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, Equipe Chimie Moléculaire et Organisation du Solide, CC1701 Place Eugène Bataillon, 34095 Montpellier Cedex 05, France.
Raehm
Laurence
L
Durand
Jean-Olivier
JO
Smaïhi
Monique
M
Werts
Martinus H V
MH
Blanchard-Desce
Mireille
M
Méthy-Gonnod
Delphine
D
Dubernet
Catherine
C
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods; D005492:Folic Acid / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*
D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D005492:Folic Acid