Molecular identification of Na(+)-H(+) exchanger isoforms (NHE2) in the gills of the euryhaline teleost Fundulus heteroclitus.
In the current study, reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR were used to clone full-length putative Na(+)-H(+) exchanger isoforms (NHE2a) cDNA from the gills of Fundulus heteroclitus. The 2480 bp cDNA includes a coding region for a protein that shows a 57% amino acid homology to rabbit NHE2. These sequences allowed data mining of available fish genome data, which revealed at least three NHE2 subtypes in some teleost species.
Edwards
S L
SL
Department of Biology, Appalachian State University, Boone, NC 28608, USA. edwardssl@appstate.edu
Weakley
J C
JC
Diamanduros
A W
AW
Claiborne
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D023781:Fundulidae / Q000145:classification / Q000378:metabolism*; D005880:Gills / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D017923:Sodium-Hydrogen Exchangers