title: Mechanisms for acute oxygen sensing in the carotid body.
abstract: Hypoxic chemotransduction in the carotid body requires release of excitatory transmitters from type I cells that activate afferent sensory neurones. Transmitter release is dependent on voltage-gated Ca2+ entry which is evoked by membrane depolarization. This excitatory response to hypoxia is initiated by inhibition of specific O2 sensitive K+ channels, of which several types have been reported. Here, we discuss mechanisms which have been put forward to account for hypoxic inhibition of type I cell K+ channels. Whilst evidence indicates that one O2 sensitive K+ channel, BKCa, may be regulated by gasotransmitters (CO and H2S) in an O2-dependent manner, other studies now indicate that activation of AMP-activated protein kinase (AMPK) accounts for inhibition of both BKCa and 'leak' O2 sensitive K+ channels, and perhaps also other O2 sensitive K+ channels reported in different species. We propose that type I cell AMPK activation occurs as a result of inhibition of mitochondrial oxidative phosphorylation, and does not require increased production of reactive oxygen species. Thus, AMPK activation provides the basis for unifying the 'membrane' and 'mitochondrial' hypotheses, previously regarded as disparate, to account for hypoxic chemotransduction.
authors.affiliation: Division of Cardiovascular and Neuronal Remodelling, Leeds Institute for Genetics, Health and Therapeutics (LIGHT), Faculty of Medicine and Health, Worsley Building (Level 10), University of Leeds, Clarendon Way, Leeds LS2 9JT, UK. c.s.peers@leeds.ac.uk
authors.forename: Chris
authors.initials: C
authors.lastname: Peers
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Wyatt
authors.forename: A Mark
authors.initials: AM
authors.lastname: Evans
mesh_terms: D000818:Animals; D002344:Carotid Body / Q000166:cytology / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D015221:Potassium Channels / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000145:classification / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D015221:Potassium Channels; D017382:Reactive Oxygen Species; D000242:Cyclic AMP; D006419:Heme Oxygenase (Decyclizing); C105245:heme oxygenase-2; D010100:Oxygen