Transformation-related protein 53 expression in the early mouse embryo compromises preimplantation embryonic development by preventing the formation of a proliferating inner cell mass.
The developmental viability of the preimplantation embryo requires the successful formation of a cluster of pluripotent stem cells called the inner cell mass. Development is variably compromised by a range of exogenous stressors (including their production by assisted reproductive technologies). Inbred C57BL/6 strain embryos are particularly susceptible to the stresses associated with embryo culture, whereas hybrid embryos are more resistant, and this is accounted for in part by the overexpression of transformation-related protein 53 in cultured inbred embryos compared with similarly treated hybrid embryos or embryos not subjected to culture. We show here that this loss of viability is a consequence of the Trp53-dependent reduction in the capacity of blastocysts to form a proliferating inner cell mass. Formation of the trophectodermal line was not adversely affected by these stresses.
Ganeshan
Lakshi
L
Sydney Centre for Developmental and Regenerative Medicine, University of Sydney, St. Leonards, New South Wales, Australia.
Li
Aiqing
A
O'Neill
Chris
C
D000818:Animals; D053624:Blastocyst Inner Cell Mass / Q000166:cytology* / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation; D003433:Crosses, Genetic; D004477:Ectogenesis; D046149:Embryo Culture Techniques; D005260:Female; D005838:Genotype; D007178:Inbreeding; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D015053:Zygote / Q000166:cytology* / Q000378:metabolism
D016159:Tumor Suppressor Protein p53