title: Preparative separation of four major bufadienolides from the Chinese traditional medicine, Chansu, using high-speed counter-current chromatography.
abstract: A preparative, high-speed, counter-current chromatographic (HSCCC) method for the isolation and purification of bufadienolides from Chansu was successfully developed by using stepwise elution with a two-phase solvent system composed of n-hexane: chloroform: methanol: water (4:1:2.5:5 and 4:1:4:5, v/v). A total of 7.5 mg of cinobufotalin (1), 8.0 mg of bufalin (2), 14.0 mg of cinobufagin (3) and 9.5 mg of resibufogenin (4) were obtained in a one-step separation from 80 mg of the crude extract with purities of 93.2%, 98.7%, 99.2%, and 99.4%, respectively. The chemical structures were determined from 1H NMR and 13C NMR spectroscopic data.
authors.affiliation: Biotechnology Application Center, Beijing Vocational College of Electronic Science and Technology, Beijing, PR China.
authors.forename: Xiu Lan
authors.initials: XL
authors.lastname: Xin
authors.forename: Junying
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao Chi
authors.initials: XC
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Lv
authors.forename: Chang Yuan
authors.initials: CY
authors.lastname: Wang
authors.forename: Ji Hong
authors.initials: JH
authors.lastname: Yao
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
mesh_terms: D002018:Bufanolides / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure
chemical_list: D002018:Bufanolides; D004365:Drugs, Chinese Herbal; C095591:chan su; C005734:bufogenin; C063451:cinobufotalin; C002471:cinobufagin; C022777:bufalin