title: A new approach to determine camptothecin and its analogues affinity to human serum albumin.
abstract: A novel and fast method for the determination of the binding kinetic data of ligand to protein has been developed. A new tool including human serum albumin-coated magnetic beads (HSA-MB) was used to determine the affinity of camptothecin (CPT) and its analogues to HSA. From the biological activity point of view, these compounds have potential anticancer activity. However, the numerous studies indicate that some of these analogues have a strong affinity to plasma proteins stopping their effective therapy. Thus, the problem of plasma protein binding behavior of CPT's analogues was the subject of this study.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, M. Skłodowskiej-Curie 9, 85-094 Bydgoszcz, Poland. mmars@cm.umk.pl
authors.forename: Michał Piotr
authors.initials: MP
authors.lastname: Marszałł
authors.forename: Adam
authors.initials: A
authors.lastname: Buciński
authors.forename: Stefan
authors.initials: S
authors.lastname: Kruszewski
authors.forename: Blanka
authors.initials: B
authors.lastname: Ziomkowska
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism* / Q000493:pharmacokinetics; D001798:Blood Proteins / Q000378:metabolism*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D008024:Ligands; D008280:Magnetics; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001798:Blood Proteins; D008024:Ligands; D012709:Serum Albumin; D002166:Camptothecin