title: [The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer.].
abstract: BACKGROUND
  It has been confirmed that nm23-H1 gene is one of the tumor metastasis suppressor genes. Up to now, the exact mechanism of nm23-H1 gebe is uncertain. The aim of this study the mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer.
  
  
  METHODS
  The wild and mutant type of pEGFP-nm23-H1 plasmids [WT (wild type), H118F, S120G, P96S, S44A] were transfected into the L9981 lung cancer cell lines through liposome method, and the complex of KSR and nm23-H1 was detected through co-immunoprecipitation and Western blot assay.
  
  
  RESULTS
  The human KSR could be detected in the nm23-H1 immunoprecipitations in all the trasfected L9981 lung cancer cell lines. But no significant difference of KSR expression was found in the wild and mutant nm23-H1 trasfected cell lines (F =0.190, P =0.938).
  
  
  CONCLUSIONS
  There was a close interaction between nm23-H1 and KSR, which was independent of the nm23-H1 mutation. Nm23-H1 involving in the Ras signaling of lung cancer may be through the KSR gene.
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Yang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Daxing
authors.initials: D
authors.lastname: Zhu
authors.forename: Wen
authors.initials: W
authors.lastname: Xiao
authors.forename: Changlin
authors.initials: C
authors.lastname: Li
authors.forename: Jiabao
authors.initials: J
authors.lastname: Ma
authors.forename: Zhixuan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Liwei
authors.initials: L
authors.lastname: Gao
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou