Serological evidence of human klassevirus infection.
Klassevirus is a proposed new genus of picornavirus that has been associated with pediatric diarrhea. In this study, we used recombinant klassevirus 3C protease as the capture antigen for an indirect serological enzyme-linked immunosorbent assay (ELISA). Four of six klassevirus reverse transcription (RT)-PCR-positive individuals demonstrated seroconversion against the 3C protease, suggesting that klassevirus infection and replication occur in humans. Additional screening of 353 samples from an age-banded serological cohort from two St. Louis hospitals indicated a seroprevalence of 6.8%.
Greninger
Alexander L
AL
Departments of Molecular Microbiology and Pathology and Immunology, Howard Hughes Medical Institute,Washington University, St. Louis, MO 63110, USA.
Holtz
Lori
L
Kang
Gagandeep
G
Ganem
Donald
D
Wang
David
D
DeRisi
Joseph L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008969:Molecular Sequence Data; D010849:Picornaviridae / Q000276:immunology*; D010850:Picornaviridae Infections / Q000175:diagnosis*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012698:Serologic Tests / Q000379:methods; D017361:Viral Nonstructural Proteins; D014773:Virology / Q000379:methods*; D055815:Young Adult
D000956:Antigens, Viral; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins