Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti.
Controlled sex-, stage- and tissue-specific expression of antipathogen effector molecules is important for genetic engineering strategies to control mosquito-borne diseases. Adult female salivary glands are involved in pathogen transmission to human hosts and are target sites for expression of antipathogen effector molecules. The Aedes aegypti 30K a and 30K b genes are expressed exclusively in adult female salivary glands and are transcribed divergently from start sites separated by 263 nucleotides. The intergenic, 5'- and 3'-end untranslated regions of both genes are sufficient to express simultaneously two different transgene products in the distal-lateral lobes of the female salivary glands. An antidengue effector gene, membranes no protein (Mnp), driven by the 30K b promoter, expresses an inverted-repeat RNA with sequences derived from the premembrane protein-encoding region of the dengue virus serotype 2 genome and reduces significantly the prevalence and mean intensities of viral infection in mosquito salivary glands and saliva.
Mathur
G
G
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA.
Sanchez-Vargas
I
I
Alvarez
D
D
Olson
K E
KE
Marinotti
O
O
James
A A
AA
D000330:Aedes / Q000235:genetics / Q000821:virology*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000821:virology*; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D023061:Gene Order / Q000235:genetics; D000882:Haplorhini; D007303:Insect Vectors / Q000235:genetics / Q000821:virology*; D008297:Male; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012469:Salivary Glands / Q000821:virology; D012737:Sex Factors; D019076:Transgenes / Q000235:genetics*
D012313:RNA