title: The noncanonical Gag domains p8 and n are critical for assembly and release of mouse mammary tumor virus.
abstract: The mouse mammary tumor virus (MMTV) Gag contains the unique domains pp21, p3, p8, and n. We investigated the contribution of these domains to particle assembly and found that the region spanning the p8 and n domains is critical for shape determination and assembly. Deletion of pp21 and p3 reduced the number of released particles, but deletion of the n domain resulted in frequent formation of aberrant particles, while deletion of p8 severely impaired assembly. Further investigation of p8 revealed that both the basic and the proline-rich motifs within p8 contribute to MMTV assembly.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n.2, 166 10 Prague 6, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Zábranský
authors.forename: Peter
authors.initials: P
authors.lastname: Hoboth
authors.forename: Romana
authors.initials: R
authors.lastname: Hadravová
authors.forename: Jitka
authors.initials: J
authors.lastname: Stokrová
authors.forename: Michael
authors.initials: M
authors.lastname: Sakalian
authors.forename: Iva
authors.initials: I
authors.lastname: Pichová
mesh_terms: D000818:Animals; D036022:Capsid Proteins; D015683:Gene Products, gag / Q000502:physiology*; D008324:Mammary Tumor Virus, Mouse / Q000502:physiology*; D051379:Mice; D017434:Protein Structure, Tertiary; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; D015683:Gene Products, gag