title: Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval.
abstract: We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.
authors.affiliation: Department of Biomedical Engineering, Korea University, Seoul, Korea.
authors.forename: Edward
authors.initials: E
authors.lastname: Kang
authors.forename: Yoon Young
authors.initials: YY
authors.lastname: Choi
authors.forename: Yesl
authors.initials: Y
authors.lastname: Jun
authors.forename: Bong Geun
authors.initials: BG
authors.lastname: Chung
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D048429:Cell Size; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology* / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009924:Organ Culture Techniques / Q000295:instrumentation*; D009929:Organ Size / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation*