title: NMR resonance assignments of NarE, a putative ADP-ribosylating toxin from Neisseria meningitidis.
abstract: NarE is a 16 kDa protein identified from Neisseria meningitidis, one of the bacterial pathogens responsible for meningitis. NarE belongs to the ADP-ribosyltransferase family and catalyses the transfer of ADP-ribose moieties to arginine residues in target protein acceptors. Many pathogenic bacteria utilize ADP-ribosylating toxins to modify and alter essential functions of eukaryotic cells. NarE was proposed to bind iron through a Fe-S center which is supposed to be implied in catalysis. We have produced and purified uniformly labeled (15)N- and (15)N/(13)C-NarE and assigned backbone and side-chain resonances using multidimensional heteronuclear NMR spectroscopy. These assignments provide the starting point for the three-dimensional structure determination of NarE and the characterization of the role of the Fe-S center in the catalytic mechanism.
authors.affiliation: Bijvoet Center for Biomolecular Research, Science Faculty, Utrecht University, The Netherlands.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Carlier
authors.forename: Christian
authors.initials: C
authors.lastname: Koehler
authors.forename: Daniele
authors.initials: D
authors.lastname: Veggi
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Pizza
authors.forename: Marco
authors.initials: M
authors.lastname: Soriani
authors.forename: Rolf
authors.initials: R
authors.lastname: Boelens
authors.forename: Alexandre M J J
authors.initials: AM
authors.lastname: Bonvin
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary
chemical_list: D001427:Bacterial Toxins; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C480854:NarE protein, Neisseria meningitidis