title: Bridging the gap: bone marrow aspiration concentrate reduces autologous bone grafting in osseous defects.
abstract: Although autologous bone grafting represents an effective tool to induce osteogenic regeneration in local bone defects or pseudarthroses, it is associated with significant donor site morbidity and limited by the amount available for grafting. We investigate the potency of bone marrow aspiration concentrate (BMAC) to augment bone grafting and support bone healing. The functional and radiographic outcome of 39 patients with volumetric bone deficiencies treated with BMAC are presented and evaluated in a prospective clinical trial. A collagen sponge (Col) served as scaffold in 12 patients and a bovine hydroxyapatite (HA) was applied in the other 27 individuals. The minimal follow-up was 6 months. Clinical and radiographic findings were completed by in vitro data. All patients showed new bone formation in radiographs during follow-up. However, two patients underwent revision surgery due to a lack in bone healing. In contrast to the Col group, the postoperative bone formation appeared earlier in the HA group (HA group: 6.8 weeks vs. Col group 13.6 weeks). Complete bone healing was achieved in the HA group after 17.3 weeks compared to 22.4 weeks in the Col group. The average concentration factor of BMAC was 5.2 (SD 1.3). Flow cytometry confirmed the mesenchymal nature of the cells. Cells from BMAC created earlier and larger colonies of forming units fibroblasts (CFU-F) compared to cells from bone marrow aspirate. BMAC combined with HA can reduce the time needed for healing of bone defects when compared to BMAC in combination with collagen sponge.
authors.affiliation: Research Laboratory for Regenerative Medicine and Biomaterials, Department of Orthopaedics, Heinrich-Heine University Medical School, Moorenstr. 5, D-40225 Duesseldorf, Germany. jaeger@med.uni-duesseldorf.de
authors.forename: Marcus
authors.initials: M
authors.lastname: Jäger
authors.forename: Monika
authors.initials: M
authors.lastname: Herten
authors.forename: Ulrike
authors.initials: U
authors.lastname: Fochtmann
authors.forename: Johannes
authors.initials: J
authors.lastname: Fischer
authors.forename: Philippe
authors.initials: P
authors.lastname: Hernigou
authors.forename: Christoph
authors.initials: C
authors.lastname: Zilkens
authors.forename: Christian
authors.initials: C
authors.lastname: Hendrich
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Krauspe
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001847:Bone Diseases / Q000188:drug therapy*; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003094:Collagen; D017886:Durapatite; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D014020:Tissue Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous; D055815:Young Adult
chemical_list: D014020:Tissue Extracts; D003094:Collagen; D017886:Durapatite